LOCUS       DAOQMC010000001        39718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_286_length_39718_cov_5.424501, whole genome shotgun sequence.
ACCESSION   DAOQMC010000001 DAOQMC010000000
VERSION     DAOQMC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 39718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39718
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_286_length_39718_cov_5.424501"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..1868)
                     /locus_tag="PKV31_00005"
     CDS             complement(396..1868)
                     /locus_tag="PKV31_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB91256.1"
                     /translation="MKKIVLFLLVTTLLGLNASGQKIRASYSDAVFNGPFTGKVIAYF
                     SKENKSPKDGAVGLESFPCFSVDVKNIKPNEEITFDDNAISYPCPLSDIERGDYNVQI
                     VWDRNLGGRSIANSPGNLFNTTQSVHISKNKKTVFKIVADRVVPNLSFRETEFVKEMK
                     APSRLLSTFHGKPMTVDAAIILPKEYYEKPEEKFPVLYMVSGYGGDYHYFSGNSTPAG
                     PLDSIPFIIVFLDGNCSLGHCVYADSDNNGPWGEALTTEFIPLLEKEYRCNGARLLTG
                     HSSGGWTVLWLQTQYPKIFVGCWSSAPDPVDFRNFQKINLYEDKNMYYDRDSSLRMVA
                     TVAGVIPWASMKNVYRMEHVVYRGEQMHSFDAVFSQKGSDGMPRSICNSITGEIDTVV
                     VSHWKKYDISLYLRHNWDRIKGDLYGKVRVSIGTGDNFLLNHAVVLLESEMKKLNSKF
                     EFVYYPGDHFTVGTPEYRKEGNQFLKQKYQEWLAKEALKN"
     gene            complement(2381..2875)
                     /locus_tag="PKV31_00010"
     CDS             complement(2381..2875)
                     /locus_tag="PKV31_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91257.1"
                     /translation="MKELRKNDSYQTFVYDFIKSDILVVCPNCSKKAIVKPGEFPAVT
                     IEQSDIKVICLSCGYNKKRIEKSDSNISSSNTGRQYVIGDGIDPFFCFPLWLRTDFEG
                     HVLWAYNIKHLDFLRDHIEAKLRERNGQELFNKSLGSRLPKWMTAKKNREILLKKIKE
                     LQNK"
     gene            complement(2934..3632)
                     /locus_tag="PKV31_00015"
     CDS             complement(2934..3632)
                     /locus_tag="PKV31_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNB91258.1"
                     /translation="MIGIITYDTPHRKTQDLVTSLIINGYSDLHLIVIPWIARENFIP
                     LFRHRPSKCANVSIEDLSDRLKISFSKHEIHNLNNFCTEKKFQHLLIGGAGLLPEELV
                     ANHKIINSHPGYLPNVKGLDAFKWAIYHEQPIGVTTHYISEKADEGRLIERRIIPIYY
                     EDSFHNVAYRIYETEIEMLVNSIKLIDNNQAKYESLADDRYVANKRMSHKNEIIMMNK
                     FEQLRQQSNSHRNQ"
     gene            complement(3871..6039)
                     /locus_tag="PKV31_00020"
     CDS             complement(3871..6039)
                     /locus_tag="PKV31_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB91259.1"
                     /translation="MLETNIKIDLSALNDQQKHAVISEDKRLLVLAGAGSGKTKTLLQ
                     KLIYLIEEKGASPSSILAITFTKNATNEMLDRLIMAADESGAYEKQLFDKRKSIAEKD
                     RERYYQQKKYKWIDGLTVKTFHGFCYNILRNYGVNEFDNKFKIIGDAKRDDEDELAKH
                     VAPETVFEVFHKLLIEACEDTEYLLNLKRYILDYIIDKIHLKQIDPNYLPKDGKYYTT
                     LDNTKVRSKSEQFIADWLYRHSIKYEYEPLLNVKDFSFTPDFYIPEANLYIEHISDKS
                     FSMKNKEEQIQKGNLLLVKTFEAMTKDSALFNHTLDKIVKNRLPSDYHKTVSINFKEE
                     FNGYHEDVKDFVRQIMRITDMIKVENIDMDSVLDNARNDQHERVRNFYELAIPVVRKY
                     LHYCTDKSYLDFNDLISRSTSLFQNHSDIANKYKSKYQYILVDEFQDVNNLQVELIKL
                     LLTDDTQLFCVGDDWQSIYGFRGSNVSYIVEFENHFTDAKTIKLNLNYRSTQNIVGAS
                     NEVIRHNKFKVEKDVQASKMSEHKIVVFAGNTEEENIQFCVDKVREILNDGISNDEIL
                     FLYRRSKMFTPYFYRFKNENIRVQGKTIHASKGLEAKVVFIIGLTEGNGGFPDIWLED
                     RIFQVIKKANHDLLMEEERRLFYVAITRAKDKLFLITQKGNESSFLKEIPATFTVRTT
                     NAIKQVVDKVILCDNCYSQLEILHKYCPYCGQPVDEQPTE"
     gene            complement(6112..6660)
                     /locus_tag="PKV31_00025"
     CDS             complement(6112..6660)
                     /locus_tag="PKV31_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNB91260.1"
                     /translation="MKRKVILYIAMSLDGYIAKQDDNIDFLSIVESPGEDYGYAEFLN
                     NIDTIIWGRRTFDKVLSFGNGVPHKDKKVYVISKTKTGKEEHAEYSNNVVDLINRLKT
                     IDGKDIYCDGGAEIVFLLLKNKLIDRIIVSVIPHLLGDGIRLFKEGNMEQALKFKRSI
                     SYPSGLVQLWYDVNLKSDEHGA"
     gene            complement(6853..7149)
                     /locus_tag="PKV31_00030"
     CDS             complement(6853..7149)
                     /locus_tag="PKV31_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91261.1"
                     /translation="MEQRIQLKHPQGKKAVSISKDKYELLKTETVKYLSKNPDGTFSD
                     ISNTIAQNFKEKKIKFNGSLNWYLEWVKLDLEAKQIIARVPKTSPQKYILTKNG"
     gene            complement(7622..7801)
                     /locus_tag="PKV31_00035"
     CDS             complement(7622..7801)
                     /locus_tag="PKV31_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91262.1"
                     /translation="MGLIKEPLDVDFVVDSRPLTKSEEKAISDFIKTDKAKRLSKKRT
                     SVLKKGRRKTKEKTT"
     gene            complement(7806..8333)
                     /locus_tag="PKV31_00040"
     CDS             complement(7806..8333)
                     /locus_tag="PKV31_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91263.1"
                     /translation="MKKQTKAGLEFVVDKLTNSIENVQSGDSFQTEVSLLTSKELKNI
                     TKKNGWNFDWKAEFKLPQREVYKLTIVNNPNIIQGLVSLEVKSDHVFMHLIESAPFNI
                     GTNKVYLGVPGNLVAFSCKLSFQRGGDGYVAFIAKTKLIDHYIKSLGAVHFGGHLMVV
                     TSETALKLIGKYFKS"
     gene            complement(8641..10425)
                     /locus_tag="PKV31_00045"
     CDS             complement(8641..10425)
                     /locus_tag="PKV31_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNB91264.1"
                     /translation="MQKYAVNQQLIETLLAWVNSGEIAIPEIQRPFVWDSSKVRDLMD
                     SLYQGYPIGYVIAWRNPNVRLKDGSLSEGKKILIDGQQRVTALTAAILGQYVINKTYE
                     RVKIKIAFHPIDERFEVQNPAILKDRTWLPDISQAINGDLFEIADQYFELNPDVDKKQ
                     VRNAFSNLMNIPKKQIGIIELAADLDIETVTEIFIRINSKGVVLSQADFAMSKIASNT
                     EYNGNELRKAIDYFCHLCIAPDFFKHIVDNDREFAKSDYFQKLQWLKTENEDLYDPDY
                     NDLIRVAFTSQFNRGRLSDLVSLLSGRNFETRTYEEDIAEQSFATLKTGVNSFINETN
                     FKRFLMIIKSAGFISPKLIRSQNAINFAYIVYLKLKELGVNSVAIERFVRRWFVYSIL
                     TGRYSGSPESAFDFDIKQISHKPIDEILGEREEAELSDAFWSASLPQSLDTSVASSPY
                     FHVFLASQVKANDRGFLSKDILVSDLISLRGDIHHLFPKDYLKKNGLDRSKYNQIANY
                     VYMQSEINIKVGNKPPKDYFEVVTTQMLNGNQLVSGLSNEQQLLDNLKMNCVPTEIQH
                     MSIDDYNDFLTLRRKLMATKIKEYYHAL"
     gene            complement(10449..11039)
                     /locus_tag="PKV31_00050"
     CDS             complement(10449..11039)
                     /locus_tag="PKV31_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91265.1"
                     /translation="MKEILLKHDKSIALVFFVLTLVFIVTAMTNEPFLNWVFERHQNQ
                     MSWFIRPVFLIPFCFFAYKRSWTGVSATIFCLFTSMFWFSKPETVSEQVSMFLQFEKS
                     WLSAQWDFKKILLALTVPVSFFALGLAFWKRSLLMGLGVVVLMATGKIVWSIYNAGQA
                     GKSILIPAITGLVLCIILIYYGFRRLEKNNNCTTNK"
     gene            complement(11249..12595)
                     /locus_tag="PKV31_00055"
     CDS             complement(11249..12595)
                     /locus_tag="PKV31_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91266.1"
                     /translation="MKIFIFYIPVLLFSFSASAQLGSPTVIDSHFSAQIRNIIAVDID
                     HDDLKDIVVSYHADSIKWYRNLGENTFSVPLFLTSTIAKPFYMDAGDVDGDSTMDLVV
                     ANNNKNHSQVVLINNNGLTPTVIDTELEVATIRACFADFDNDGDLDIVANSDLMITLY
                     LNNGSGVFGNRIVLLDGAEFYNMTVADFNGDNFPDIGLCSSDGSQLFLNNTMGGLSSP
                     NTLDINLCLFVTHTDIDNDGQMDIIFPGSSSNQYNVYRNTGGGTFALNQSVTFNVANI
                     QNPPFAIANMDDDAFPDAVYSSINDKRALFWQANDGTGTFQNQQLINNDEVFDAIYLA
                     DMDNDGDNDILWYGFNFDLSLKKLGIIENTTNTMDINDNLNTGSIKIYPNPGSTELNI
                     EFQDEISTVELYSMNGVKIATYNSAQFDISNLANGIYILKPTTIKGTTFSAKFIKK"
     gene            12909..13556
                     /locus_tag="PKV31_00060"
     CDS             12909..13556
                     /locus_tag="PKV31_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNB91267.1"
                     /translation="MKAIVIGGTGATGAYLLQELLASDRITSVTALTRRAISVDHPRL
                     NQQIVDFEQLEDVSISGDLAFSCLGTTRKAAGSKKAQWRVDHDYQLAFARMARKANVP
                     VFTLMSSMGSDKNSALFYSRMKGTLEEAIIHLDFDHTYIFRPGMLERPGSDRLMERWV
                     VSLMKGMRRLGMPQKYGPSHISIVAKAMVAASLNHDDTLKMFDVADINTLAQEKY"
     gene            13556..14479
                     /locus_tag="PKV31_00065"
     CDS             13556..14479
                     /locus_tag="PKV31_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNB91268.1"
                     /translation="MMTMETARKNYYISGYAALSVFFLVLYYVTNLPIFKQWEHYLAL
                     IQKFTLSLSLISAIFLTGRIIERLIDSRTETEGARHNLFRITRLLTIVFVLIVVVSFL
                     FQNLYAVAISFGLISLVLGFALQAPTTSFIAWLFIIFRKPYQVGDRVQIGTLRGDVIE
                     ISYLDTILEEVSGTYLGNDRKSGRLIHFPNSLILTSQVVNYSGPFDDFIWNETAVQIS
                     FTSDLTFVEYCLKEAADKDFMEQYPGKPMDDLHAAVYFRVNTFAWLEAVVSYPVEPED
                     TTGRRNRILKYGLASLNAHPDKVGFPEGTKR"
     gene            14988..15332
                     /locus_tag="PKV31_00070"
     CDS             14988..15332
                     /locus_tag="PKV31_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB91269.1"
                     /translation="MSSIQEFNDYRQRMNDRIMAQDNKVIKRFWSLDNQTYQAEALDV
                     RTKEMLGLVASMVLRCDDCIKYHLQRCHEEGLTTEEVFEVLSVAIIVGGSICIPHTRR
                     AVEFWDELNNQK"
     gene            15336..17612
                     /locus_tag="PKV31_00075"
     CDS             15336..17612
                     /locus_tag="PKV31_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB91270.1"
                     /translation="MASISFLDELNEVQYKAVTTIDGPVMVIAGPGSGKTRVLTYRIA
                     FMIEQGIKPQNILTLTFTNKAAREMKERLEKTVGPEAKKVWAGTFHSIFAKILRFEAE
                     KIGFPASFTIYDTDDTKSMISHIVTEMNLDSKLYSANMIRNRISSAKSALLSPTAYLQ
                     NEELMNQDKLNKVPLIGKIYERYARRCKEAGSMDFDDLLYNTFVLFHRNPENVLEKYR
                     ERFQYLLVDEFQDTNFLQYAILKKLVKYPGSKENLCIVGDDAQSIYAFRGATIDNILD
                     FNNDFKGLRTFKLEQNYRSGKFIVNAANNVITHNRRQIKKEIWTSQSDSRRIGLIRAL
                     SDVEESRKVVDAIFEHKNRNHITNSEIAVLYRTNAQSRIVEEQLRRANIPYRVYGGLS
                     FYQRKEIKDVLAYFRLVVNHRDEEAFRRIINYPKRGLGDTSIEKLFAYAAEKGLSFWE
                     AIDDQPLPAKAQAVLNEFRKMITGLHQFAMNNDADKAANHIYQKSGLSVVLKADTTPE
                     GKGRLENITSLLDGIKEYVENDEEVLDENGDLVIEKGRGLVDYLQNISLITDFDQESD
                     NGDHVNLMSVHSAKGLEFDSVFVVGLEENLFPSFLSLKSESEIDEERRLFYVAITRAR
                     KLLTLSYATSRYQYGKLAYNEPSRFLDEVGEDNLVTPVHQARNLVEDRAPRASVSGIF
                     TKKQPVKANVAIHDIVPSPVSAFQVGVTVVHPRFGQGTIKAIDGTSDNKVAIIQFDTG
                     DDSDKRIMLKFAKMQVVG"
     gene            complement(17869..19071)
                     /locus_tag="PKV31_00080"
     CDS             complement(17869..19071)
                     /locus_tag="PKV31_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNB91271.1"
                     /translation="MSNPVYFEEHLPKTEMLEVKKKHANLSIGIIREQMEMENRVALV
                     PASVAYLVGNNHKIVVETGAGMRSNFSDHEYSEAGAEIVQSTKEAFKCDVILKVAPPT
                     HEELDNFRPGQILISPLQIPLINSDYINKLRQKRVIALAMEYMMDDDGSFPLVRIMSE
                     LAGISAILTAANLLTTTSGGTGVLLGGISGVPPAKVVILGGGVVAEYAVRTALGLGAS
                     VCVFDNNIYKLMRIQARVGQRLFTSSINPTYLGQALSDADVAIGAVHSKAGRTQLIVS
                     EEMVARMKPGSVIIDVSIDQGGCFETSEVTSLDRPTFEKHGVIHYCVPNIASNVPRTA
                     SVAISNMITPILAKAGSAGSIENVLFSHINLRNGVYTYKGCLTNKYLSDRFAIKYTDL
                     DLLMTSTF"
     gene            19825..20412
                     /locus_tag="PKV31_00085"
     CDS             19825..20412
                     /locus_tag="PKV31_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB91272.1"
                     /translation="MHLKNITVFCGANSGANPEFTLIARKVGSLLAGRGIGVVYGGAK
                     IGIMGAVADGALARGGRVTGILPHFLSAREIAHDGLTELVVVDSMHERKLKMYAMSDG
                     FLVLPGGFGTMDELFETLTWAQLGLHQKPIGILNTGHYYDELIALVERMAAEMILKDI
                     YRSMMIVGDEIEELLSKMENYRAPQVEKWITPDKT"
     gene            20420..20962
                     /locus_tag="PKV31_00090"
     CDS             20420..20962
                     /locus_tag="PKV31_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB91273.1"
                     /translation="MSIDLQQPLENDFVGLVPLQVGDFELLYLVASNPKVWEQHPNKD
                     RWKRDVFLTFFQGAMESGGAYRIVNKSDGKTIGSTRFYGHDPDDESIMIGYTFYDPEY
                     WGKGYNPAVKILMMDYIFRFVSKVFFHVGAVNKRSRIAIERLGAVKVGEKEIEYFGEK
                     AAVNIIYCIEKENFLSRYHH"
     gene            complement(20968..21990)
                     /locus_tag="PKV31_00095"
     CDS             complement(20968..21990)
                     /locus_tag="PKV31_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNB91274.1"
                     /translation="MGQRIFPAEWARQSGVAFTFPHANSDWGPYLEIVIPTFVEIITL
                     VSRHEKVLVVCQDAELVSTLLKDAVQENLILVELPSNDTWARDHSAITILEEGHPSLL
                     DFIFNGWGLKFPANHDNLITKRLQAKGIFGDLAVHTYGLAIEGGALESDGTGTLLTTS
                     ECLLSPNRNPHLNREMTENTLREILGVNRFLWLDHGYLAGDDTDSHIDTLARFCSEDT
                     IVYVSCDDKEDEHYESLKAMECQLKTFARSDGSPYNLVPLPMAPARFHPEGYRLPATY
                     ANFLIINGAVLLPVYGDPKQDDMAVRVIQKCFPNRKIHPVDCNSLILQHGSLHCITMQ
                     FPEGVL"
     gene            complement(22164..25649)
                     /locus_tag="PKV31_00100"
     CDS             complement(22164..25649)
                     /locus_tag="PKV31_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB91275.1"
                     /translation="MKNILLSVLLIWSATEVYSQCSVDAVNPDLLNQTVCEGESIIPI
                     IINITGSFNVDGLSGTGLQTSFSQNNSQLTIFGTPTVDLINYTVSCSWIGFTGPSGTS
                     VSGTITRVPLPTVDQPSNIFECGGTQVTVIFSGSNATSYSWTNSNTSIGLGSSGSGNI
                     NFVASNVNGTQTATITVTPSNTSCSGSPKTFTITIYPSPDISGNNWVCEGNQEQLTGS
                     GMPAQSNPWTSSNPDVATVDNNGLVTGNVPGTVIITYTALNGCFDQIGFTVYPKAYAG
                     IDQNILCWHLEYAYMAASGTGQWELLTTDPPGLNLNIIDPNDPNTGVNNFSGPGTYIL
                     RWTNEFGCFDEAIITAQDICPCENPPEIILSIDEKSVCGAQAITVDSNIFLNASTLSF
                     FHNGSGTLEHLVFPGNSFMFTYTPTPSDFGKTIQITIVTDDNDGSGPCEPATAIFFLH
                     IDGNPEVGPDIQVDCASRDSAIISATSGEGLWILIDTDPPGMTLDIVDPSNVKTTIKN
                     FSGTGYYTLIRQLTSGCSDTLTVEAKNDCDCTAQPDLNLSATSFTTCDLKTISVPNNT
                     YFNATTVLVSHNGSGNASVTHTSSNTFTFSYTPTQADTGKTITLTLYTDDPDMNGPCY
                     ADTMKFVLQYHLIPHVSSDVAEVCTKSGGVILSASGSGNWNLVSTTPAGLTVSISEPT
                     SANTLVTSFSGPGSYILRWTTSEGCANDLAITAVDCPCAPDIVLSTEGAYCGQNNGLI
                     HVKVSNATYPYSCSLSGAKNKTIDHVSTDTVIFDHLGPGTYKVSVIDAAGCQSASVTQ
                     VHDAGNTLSLILGSVKHSFCGDHNGSFSAYVTGGRPPYTFTVNGSMVSYPVSMDNLSA
                     GSYMLSVVDNAGCTKDTTITLLESNPFTANLTDSGFDCNSATGIISVTAEGGRPPYAY
                     ALNHGVYGENPVFTGLTPDVYTISVVDVDLCYTELTITPFIKADFSAVDDYLQWHFQY
                     NPLIIDVISNDSITNPADYSVRPLSEIITDDDGNEIGKLFNREVGDQRMEFYFDGARQ
                     NLYPELIKMPYLLCETDCPVKCDTGIIYLLQKVPCTDDNGGFVNTITPNGDGRNDVLI
                     IPNFPKCNVTRMAIRIFNRWGNAVYENSEYGNEWDGKNNDGSPLPEGTYYYVLSLKTN
                     DGKDYVIKNFVEIIR"
     gene            complement(26666..26821)
                     /locus_tag="PKV31_00105"
     CDS             complement(26666..26821)
                     /locus_tag="PKV31_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91276.1"
                     /translation="MRRTPVIPQIKGGDPHPVTQPPGNGLPIIKSAEKTVQDDNIRAT
                     VAVLLEI"
     gene            26762..27430
                     /locus_tag="PKV31_00110"
     CDS             26762..27430
                     /locus_tag="PKV31_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB91277.1"
                     /translation="MSDRMRVTTFDLRNHGRSAHVPDFSIELMASDVYEMLQALHIGS
                     AVIIGHSMGARVAMCFSLAYPAVTDGLIAVDMATKKYERGHDDIFAALEAVDLNAGSR
                     KEVENILAKRIPDEGTLQFLMKNLSRREGGIGFEWKMNLPVIKANYETILQPIASEIV
                     YQGPVLFIRGGNSNYVADEDLADIKKFFPYAELATVAGAGHWVHAERPDAILTLIEQF
                     VAEL"
     gene            complement(27448..30552)
                     /locus_tag="PKV31_00115"
     CDS             complement(27448..30552)
                     /locus_tag="PKV31_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB91278.1"
                     /translation="MKKHQSALLYLLVIALIFNSCASPHKKKEDVGLLGRAYQNMTAY
                     YNGYFNANELLDAAMLEIESKHPDNFQEQLIIYPYLLDQDSTTASGTLNAAIEKLKKD
                     IYLHPASHWVDDSYFQMGKALFLKKDYERAENAFKYVVQNYSEAQLARDKANRMSPGK
                     KKKAIEKERQEVKKEKEAEKEKVREEKADAKKAKEKEAKQKAADRKKAAKERKKAAQK
                     KAKERKKAAAAKKKGKSTPKTAPKTTKAPESKPAGTTTPDSKPTDNKTEVKTEKADTQ
                     PVKSVKEDEDNVVATKKDKPSIRPKNYLFKHRPIFQQAQLWLAKTYIQRSNYEDGERI
                     LRSLLNEGGTYDLVRREALLVSAQVDMKKESYTQAIENLNKAIPMVKEKKRRARLTYI
                     LGQLYIKAGNYDMATQAFERVERLKPSYEMAFNASLSKFGCSVATGKISDEELNSRLN
                     KMLKDLKNDEYQDQIYFMLADINLRNNDIAAGIKNLQKGIQVSKKGPEAKAESYLKLA
                     DIFYKRDQFDLAYHYYDSTATAYPKTKERFKEIVDRRDNLKDIAYNIETIQYQDSLLM
                     VGRMTPEEQEALAKKLIKEAREKEKLATASNTGSTFNPKVGRGGSQPAAIGGGPAFGN
                     PGQIGGAKPTAFFAYNDKTLKDGKRQFDKDWNNRPLADNWRYSALLQSFDNNTSTDKE
                     EGLSALFISKSEIKKVLADVPTDAATIARSEAAIMDAMNALGVLYPDKIERYDKAIEV
                     LEDLLQRFPDNPHEPQAFYQLYFAYMMKGNSQKANYYADLLKTKYNGNQYSLYFSDPQ
                     YIKNKADKEAELNDYYLSTYTSMEKGDYATAFNMAQKSDSIFGIKNILRPKFAMVTAM
                     SLGYLKGKETYISALKDLVQAYPNTMEEKRAKEMLRLLGTGSNANEQQLAEAEKIYKA
                     TDADQHFILIIFKPEITGTEEVRAQVTDYNTRYFSSANLKIATITLKDGNQDRQVLLV
                     RTLDNKIKAMEYYNQALNKKGDFISARFPYDMYAISNANYRELLRLRSAELYDIWFEK
                     YYRN"
     gene            complement(30766..30993)
                     /locus_tag="PKV31_00120"
     CDS             complement(30766..30993)
                     /locus_tag="PKV31_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91279.1"
                     /translation="MHNNWNFLRILRLVLGAIILVEGIRSGEVFFLIFGAIFTLMPLL
                     NVGCCSAAYCNPRQQRTSAYKDEEIHFEEVK"
     gene            complement(31091..31303)
                     /locus_tag="PKV31_00125"
     CDS             complement(31091..31303)
                     /locus_tag="PKV31_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNB91280.1"
                     /translation="MKKNLGTGDKFIRLLLAVLILALYLTNAISGNMALLLLFFAIVF
                     ALTSYISFCPIYQIFGINTCKAKEQK"
     gene            31482..36587
                     /locus_tag="PKV31_00130"
     CDS             31482..36587
                     /locus_tag="PKV31_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB91281.1"
                     /translation="MTWSKVYFISYFYTKFTEKYFLFHVIKNTPNEPHHQIFFGIAMI
                     ALAHMVNGQSVKPIHTVHGSFTPPPKEVVLQNHESRNPSREKIVWIQLADDTINGFRD
                     SIVNRLSPVDFIDDRTFAVRVDAVGLKDLAANEAVVSIFEMPAALKLDRKVVDADDSE
                     KLEVRISLYGAMSDSEIENLIGPVKIISHHVSGDKTYLVIACDRPSALEIAAGAQVRA
                     ISENISAFTPLNFYKRSLEAVQGANASLQNGGLGLNGAGITIGHGDTGGFFHEDLNDR
                     VVHFALDSVTAHAPHTAGSMIGAANLKDKHRGIAQEATLVNATYFDIISYDNQFYNDF
                     NVRVTNNSYAVLDKNIDCNSMGEYNIFSEEADQSTYEYQDLLHVWASGNSGTLACGDH
                     IAGYGTVLSGPQCNKNGITVGAIWRLEGTPLADFSSKGPTRDGRIKPEIVAQGTNVTS
                     CNGKNDYYTTAGTSMAAPGVTGAIALMQEAYKKANNGKYPSAALLKAILINTCDELLG
                     EGPEFSVGYGKVNTMKAAREAGKDHHDIGSAFSGSEDIYHFDIPEGQAKAVFSLAWTD
                     PPANPLSTRQLINDLDLIVVGPTGQYLPWVLNPNPDYVFNAAVRGVDSLNNLEQVSIL
                     NPVAGKYEIIIRGKRVAGSQEYALCYRYEAKKMELYGPFGGERYIPGEFQYFQWGSPY
                     NTGDSTVISLSLDDGQTWTELGRRAENIRTFNYIMPNVTSDKARLMIHSDKMTGDTSG
                     RFTICPSPKFNYISTCRGEADLSWPTVDGASAYNIYRKTGAYMEIVATTTDTTYRLAG
                     LNPDIDEMISVAAVLPDGGVSRRDHTQFVKTLGVQCDHKFDLEILEITSPERFGRELT
                     STALTNEEKVSIRIVNKGLEAAQGFSIHYVIDNIETVEVFNELILPGETAEYTFQQPA
                     RLSEAGNHQLRVWIDYNFDSPYHSDNAKEILIRQIPNKEIVLSRELSSTFHLGFDDQH
                     TGVARNGDKGILGVDEIDFHSNNPRGRARMVVPSGFNKSGKGALTMDVDYQGTSVQNE
                     AIFTFNLSKYTLDDDINLELSYLNQNSPLKVDSLTAKLWIRGSDSDIWVEAYNLKENE
                     ARAGTYTSIRSLRLDKLLRDANQQFSTSTQLKISQLVDGPAVARDGFSGYTFDDLYLY
                     QSQMDMQVTRIISPAPNSCGLTADEEITLEIANTSNEPVDSLSLWYSVDGVVTGPIDI
                     PPMLGTEVRTISGLPADLSTIGAHTIKVWVAAPGDTYRANDTLSGYTVYNNGFVGQFP
                     YLATFEAGNETWISSGFQSSWIRATPDKDNLQGAANGDFAMVTSSDGRYNGYERSTLT
                     SPCFDLSGLQEPYLSFSFWHDTEVEYDSCWVEYSVDGSAWKILENTHRSINWYNRPFA
                     WDGQDDHWHVAGLPLPVQLFTDRSGIQFRFVLYADENVQKGGMAIDDIHIYEGPDIYV
                     GGDNKVSFLANQHWQDIVVDGRTIAAIKSEDPQPEPFTVGVHFNTGTEQRHDQSQYYL
                     DRSLSVNTASGTAKPYNIRFYFTDEEAERIIHAGPGYHAPNSAYQLGLTSISSEDNDG
                     VFGNEVNPEYDFFSFKRTQLVPYKDGYYLEFRAKGPGEFFLNSGGKEFNLPLGNTTSN
                     VFGVGLAGDNLTARPNPFSDEFVIYLETAQKSKIYRVELVDVSSRIVNTYMVPGESLS
                     EGFPVRTRLPAGVYLIRIHGSDLHAVSRLVRVD"
     gene            36772..37947
                     /gene="mnmA"
                     /locus_tag="PKV31_00135"
     CDS             36772..37947
                     /gene="mnmA"
                     /locus_tag="PKV31_00135"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNB91282.1"
                     /translation="MKRVVVGLSGGVDSSVAAYLLKEQGYEVIGMFMRNWHNDEVILS
                     NECCWVEDSNDALLVADKLGIPYYVIDLSAEYKTRIVDYMFAEYEKGRTPNPDVLCNR
                     EIKFDVFLEAAMKLDADFVATGHYCRKTTDANGRHHLLAGKDGNKDQSYFLCQLNQDQ
                     LAKALFPIGDLLKSEVREIARREGLVTAEKKDSQGLCFIGKVDLPTFLQQQLQPRKGD
                     IIEIPQELERLVQYHEIPVSPENIEALSSPFTFEATEGIKVGEHRGAHYYTIGQRKGL
                     HIGGRPYPSFVLQTDTRHNIVFSGQQSDHPGLNRYALKIEADEIHWVNPDYELSPDSS
                     IEMEVRIRYRQPLQKATLIRKPDALYILFEEMQKGIAPGQFAAWYLGEELIGSGVIA"
     gene            38599..39249
                     /locus_tag="PKV31_00140"
     CDS             38599..39249
                     /locus_tag="PKV31_00140"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNB91283.1"
                     /translation="MNTKTKLLAIIFTVLIIDQALKIYIKTHFQYGEDMPLFGSTWAY
                     FNFVENKGMAFGLSLGGDYGKLALSLFRIVAVIFLWIYISRLIKAKAHMGLLVSFALI
                     FAGAVGNIIDSAFYGLIFSESSYHGGVATMFPEGGGYAGFLHGKVVDMFYFPIIDTVL
                     PDWIPFWGGQRFEFFKPVFNVADTSISVGVGILLVFYKRFFGEHEETATTDVSNKE"
BASE COUNT        10674 a         8629 c         8563 g        11852 t
ORIGIN      
        1 ccggaaactt ccaatctgcg cagaatttca tccgcataag cgcaacttgg gttaacgcta
       61 acatctgtat atacaccata tggcgtatat ctaacaaata aagttacatc tttttctcat
      121 acagccaaac caccctcgtg ctctctaaat ctcttcctct acctacgcaa caatatggat
      181 tttccgtcgt cttcagtgag ttatagccca aaagctcttg tacgatgcgg atatccttcc
      241 cgccccggat cagatgcaca gtatatccat gccgccgggt ttgcgccgtg gcctatagtg
      301 caatagcgac ctttccggaa tagctgcaag ggtgttcagg atattaatga gagcatgtgg
      361 aacttgtcgg tacatccatt ctcccggtac ttctcttaat ttttaagtgc ctcctttgca
      421 agccactcct gatacttttg ctttaggaac tgattgccct ctttcctgta ttccggcgtg
      481 ccaaccgtga agtggtcacc cgggtaataa acaaattcga atttcgaatt taattttttc
      541 atctcggatt ccagcagtac gacggcatgg ttgagcagga aattatcgcc tgtgcctatt
      601 gatacacgga cctttccgta cagatcccct tttatcctgt cccaattatg tctgagataa
      661 agggagatgt catacttctt ccaatgtgat accacgacag tatcgatttc tcccgtaata
      721 ctattgcata tacttcgtgg cattccgtca cttccttttt gactgaatac agcatcaaag
      781 gaatgcattt gttcaccccg atataccaca tgctccatcc ggtacacatt cttcatggac
      841 gcccagggaa tgacacctgc caccgtggcc accatcctta acgaactgtc tctgtcataa
      901 tacatatttt tgtcttcata taggttgatt ttctgaaaat tccggaagtc aaccggatca
      961 ggggcgctcg accaacagcc gacaaatatt ttcggatatt gtgtctgaag ccacaagacg
     1021 gtccaccctc cggagctgtg accggttaat aatctagccc cgttgcacct gtattccttt
     1081 tccagcaatg ggataaattc agtagtcagt gcttctcccc agggaccatt gttgtcactg
     1141 tcggcatata cacaatgccc caatgaacaa ttgccatcca gaaaaacgat aataaaggga
     1201 atgctatcca ggggtccggc aggggtcgaa ttccctgaaa aataatgata atcacctcca
     1261 tacccggaaa ccatatatag caccggaaat ttttcctccg gtttttcgta atattctttt
     1321 ggaaggatga tcgcagcatc caccgtcatt ggctttccat gaaatgtact gagaagcctg
     1381 gatggcgctt tcatttcttt cacaaattca gtttcccgga aggatagatt cggaacgacc
     1441 cggtctgcca ctattttaaa aacagtcttt ttgtttttgg aaatatgtac gctttgtgtg
     1501 gtgttgaata agttgccagg actattggca atagatctgc ctccgaggtt tctgtcccaa
     1561 actatctgaa cattgtaatc accccgctct atgtcggaca gaggacatgg gtaagaaatg
     1621 gcattgtcgt caaaagtaat ttcttcgttt ggttttatat ttttcacatc aacggagaag
     1681 cagggaaagg actcaagccc aacagctcca tctttggggg atttattttc ctttgaaaaa
     1741 tatgcgatga cttttccggt aaatggtccg ttgaaaacag catcagaata acttgccctg
     1801 attttttgac cggatgcatt aagccccaaa agggttgtaa ctaaaaggaa gagaacaatt
     1861 tttttcatga taattttttg tagtcgctaa gcagactgca caatttccgc tacatatttg
     1921 gttttatagc tgaatggagt catatgaaca aataaaatta ctccttttcc tttaatcatc
     1981 atccattcct gagataaaat tgcttcttcc atttcttaga ccaattatac accaactatt
     2041 ctgaggtgta gtatctgtac aggatgaaca aatcaaagga caggctctcg ttgttgtata
     2101 aattctttgg ccaacccgcc aatttaggcc gacttcctca tgacaaaata ttacttttcc
     2161 tttcttattc ttccggtcgg tgtactttgt ggggatgggt tttgggtgtc ccgttgatga
     2221 agtcgggaaa aatcatttgt cgctccatcc gggctaattg aagattcatg gttgaaggaa
     2281 tatcccttac ttcctatgcc caaccgtctt gtccgtaaaa tgtcttcacc ctgtgcctca
     2341 cgctcttgtc ccctcgcttt gcggtagcgt ttctgtccgg ctatttgttt tgcagttcct
     2401 ttattttctt cagaaggatt tccctgtttt ttttagccgt catccatttc ggaagtctgc
     2461 tgcctaagct tttattaaat agttcctgtc cattacgttc tcttagtttt gcttcaatat
     2521 gatccctcag aaagtcaagg tgctttatgt tatatgccca taaaacgtgt ccttcaaagt
     2581 ctgtccttaa ccataatggg aagcaaaaaa acgggtcaat gccatcgcca atcacatatt
     2641 gccggccggt gttcgatgag gagatatttg aatctgattt ttctatacgt tttttattgt
     2701 atccacaact caggcatatg actttaatgt cactttgctc aatggttacg gccggaaatt
     2761 ccccgggttt tacgatggct ttttttgaac aattcggaca aacaactaaa atgtcagact
     2821 ttataaagtc gtacacaaat gtttgatagc tgtcgttttt tctcaattct ttcatttgaa
     2881 tttcaatcta aacaggcgtt gtctccttac ggccaatgta tggtgtactg tttttattga
     2941 ttccgatgtg agtttgattg ttgcctcaat tgttcaaact tgttcatcat tattatttca
     3001 ttcttatgtg acattctttt attcgccaca tatctatcgt cagccaatga ttcgtactta
     3061 gcctgattat tatctatcag cttaatacta tttaccaaca tttctatttc ggtttcataa
     3121 attctataag ccacattatg aaaagagtct tcatagtata ttgggataat tcttctttct
     3181 attagccgac cttcatctgc cttttcagaa atataatgcg tagttacccc tattggttgc
     3241 tcatgataaa ttgcccactt aaacgcatct aatcctttaa cattaggcaa atatccggga
     3301 tgagaattaa ttatcttatg atttgccacc aattcttccg gtagcaatcc tgctccgcca
     3361 atcaacaggt gttgaaattt tttttccgtg caaaagttgt tcagattgtg tatttcatgt
     3421 tttgaaaagg atatttttaa tctatccgaa aggtcttcaa tactaacatt tgcacatttt
     3481 gaaggcctat gcctgaatag gggaataaag ttttcccttg caatccaagg aataactatt
     3541 aaatgaaggt ccgaatatcc attaatgatc aggcttgtca ctaaatcctg tgttttacga
     3601 tgtggtgtgt cgtatgttat tatacctatc attgtatctc ctctcttatt taatttattt
     3661 tacgtccaac agctcccggc ccggggattt gatcacagcc ctcagctcca gccgaagagc
     3721 taatgctcaa atgtagtaaa aagtttctaa cgaagcattg tgcacgagat tttgcccacg
     3781 ctagggtgaa ttaattcttt ggcattctcc ttttgtcggt gcgttgccta aaatttggtt
     3841 cttttgctcg aggcgagggg tcgggtttcc ctattcggtt ggctgctcat caaccggttg
     3901 tccgcagtag gggcaatact tatggagtat ttccaactga ctataacagt tgtcgcaaag
     3961 tattaccttg tcaactactt gcttaatagc gttggttgtt cttacggtaa atgtcgccgg
     4021 aatttctttc agaaaacttg attcgttgcc tttttgtgta atgagaaaaa gcttgtcttt
     4081 ggctctggta atagctacat aaaacaaacg tctttcttct tccatcagca ggtcatgatt
     4141 ggctttttta atcacctgaa aaattctgtc ttcaagccaa atgtccggga agccgccatt
     4201 tccttctgtc aggccgatga taaaaaccac ttttgcttca agtcctttag aagcgtggat
     4261 tgtcttgccc tgaacgcgaa tattttcgtt tttgaatcta taaaagtagg gggtaaacat
     4321 tttgcttctt ctgtacaaaa acaaaatttc gtcattactt ataccatcat tcagaatctc
     4381 ccggacctta tcaacacaga attgaatgtt ttcttcttct gtattacccg caaaaacgac
     4441 gattttatgt tctgacattt tagaggcctg cacatctttt tcaaccttaa atttattgtg
     4501 cctgatcact tcgttgcttg cgccaacaat attttgtgtg cttcgataat tcaaattgag
     4561 tttgattgtc tttgcatccg taaaatgatt ttcaaactct acaatataac tcacattaga
     4621 gcctctgaat ccgtaaatac tttgccaatc gtccccaaca caaaacagtt gcgtgtcgtc
     4681 agtaagcaat agttttataa gttcaacctg tagattattt acgtcttgaa actcgtcaac
     4741 caaaatgtat tgatacttgc tcttgtattt gttggcaatg tcgctgtgat tttgaaacag
     4801 ggaagtactt cttgaaatca aatcattgaa gtcgagatag gacttgtcgg tacaataatg
     4861 caggtatttt cttacgacag ggatggctaa ttcgtaaaaa ttgcgaacac gttcgtgctg
     4921 gtcatttcgt gcattgtcca aaaccgaatc catatcgata ttttcgacct ttatcatatc
     4981 cgtgatacgc ataatttgcc ggacaaaatc ctttacatct tcatggtacc cgttaaattc
     5041 ttctttgaaa ttaatagaaa ctgttttgtg atagtcggaa ggcagtcgat tttttacaat
     5101 cttatcaagc gtatggttga acaatgcaga gtctttggtc atcgcctcaa aggttttgac
     5161 aagaagcaaa ttccctttct ggatttgttc ttctttgttt ttcatagaaa aacttttatc
     5221 gctgatatgt tcaatgtaca gattggcttc ggggatgtaa aagtccggtg taaatgaaaa
     5281 gtcttttacg tttaatagtg gttcatattc gtatttaatg ctgtgacgat acaaccaatc
     5341 ggcaataaat tgttcggatt ttgaacgaac ttttgtgttg tcaagggtag tataatattt
     5401 accgtctttc ggtaagtaat tggggtcaat ttgctttaaa tgaattttat caatgatgta
     5461 atcgagaata tatcgtttta gattgagcaa atactccgtg tcttcacatg cttcaatcag
     5521 tagtttgtga aaaacttcaa atactgtttc cggtgccacg tgttttgcaa gttcgtcttc
     5581 atcgtcccgc tttgcatcgc caatgatttt gaacttgttg tcaaattcat ttaccccata
     5641 attccgtaag atattgtagc aaaagccatg aaaggttttg actgtcaacc cgtcaatcca
     5701 tttatatttt ttctgttgat aatatctttc tctgtctttt tctgcaatac tttttcgttt
     5761 gtcaaacagt tgtttttcgt aagccccgct ttcatctgct gccatgataa ggcggtcgag
     5821 catttcgttt gttgcgtttt tggtaaatgt aatagcgaga atacttgatg ggcttgcacc
     5881 tttttcttca atcagataaa taagtttttg caaaagtgtt tttgtttttc ccgagcctgc
     5941 cccggcaagg accaacagcc ttttatcttc gctaataaca gcatgctttt gttggtcgtt
     6001 cagcgcactt aaatctatct ttatgttagt ttccaacatg caattaatat tttacaggac
     6061 aaaagtccga aatatttttt ttaaaaattg gcgagagttt tcccgggtat tttatgctcc
     6121 atgctcgtca cttttaagat taacatcata ccaaagctga acaagcccgg aaggatagct
     6181 aatacttctt ttgaatttta atgcctgttc catgttgcct tctttaaaca gtcgaatgcc
     6241 atcgcccagc aggtgaggaa ttacgctcac aatgattctg tcgattagtt tattttttaa
     6301 tagcaggaaa acgatttccg ccccgccatc gcaatagatg tctttgccat caattgtttt
     6361 cagtcggttt attaaatcaa ccacattgtt tgaatactca gcgtgttctt ctttaccggt
     6421 ctttgtttta gaaataacat atactttttt gtctttatga ggaacaccgt tgccgaagga
     6481 taaaaccttg tcaaaggttc ttcttcccca aatgattgtg tctatattat tcagaaattc
     6541 ggcatagcca tagtcctcac ccggtgactc aacgatggat aaaaaatcaa tattgtcgtc
     6601 ttgtttggcg atatatccat caaggctcat tgcaatataa agtatcactt ttcttttcat
     6661 ttgcggttgt ctatatgctt gcttctaacg gtttgggtat tgccgaaggc ggcacatatt
     6721 accactaaac ttcagccgaa gacgagacca taaatttagc aaaaaatgtt caaaagaaga
     6781 cgttttctgc cgcttttgcc aaatgagctg ttagtggttc gttgttcttt cttgtccatt
     6841 gtccacattg tttcatccat tttttgtcag tatgtatttt tgaggtgatg tctttggaac
     6901 tcttgcaatg atttgttttg cttcaaggtc aagttttacc cattcaagat accaatttaa
     6961 ggagccgtta aactttattt tcttttcctt gaaattttgg gcaatagtat ttgatatgtc
     7021 tgaaaatgtt ccgtcagggt ttttcgaaag gtactttacg gtctctgttt tcaacagttc
     7081 atacttgtcc ttgctgatgc taaccgcttt ttttccctgc gggtgcttga gttgaattct
     7141 ttgttccatt atgtctgttt ttttattgtc gtctgtttga tgtttgcgcg gtgtctgttt
     7201 agaatgacca ctaacatctg tatatacgcc atatggtgta aattttccgt ataaatataa
     7261 tcacttccct caaattttcg taatcgacct tccaagcaca tcttttttaa gccttcaacg
     7321 atcatcccta atcatcaaaa tatgcaccat cacgaagtcc gaaaagttgg cggaccgcta
     7381 aaggatataa gaatcaaact ccaataatat tgggtgaaaa tgttccataa aattccatct
     7441 cactatatca tccggaaaca tgactaatct cctgatatat ccgaaaaaat gaggtgaaga
     7501 taccagatcg gttatgctga gcttaaatcg ctatttactt cactttgttc agtgagatcg
     7561 ctttgctaag ttcgcttcga tcaaggagat taagccttat ttgttttagt ggaagtgaag
     7621 cttaggttgt tttttctttt gtttttcttc tacctttttt caaaacagat gttctttttt
     7681 tagacaagcg ttttgcttta tcggttttga taaagtcgct tatcgctttt tcttctgatt
     7741 ttgtcaatgg tcgtgagtcc actacaaaat caacatccaa aggttctttt attaagccca
     7801 taattttaag atttaaaata ttttccaatt aatttcaatg cagtttcgga tgttacgacc
     7861 attaaatgtc ctccaaaatg tacagctcct aaagatttta tataatgatc gatgagtttt
     7921 gttttagcaa tgaaggcaac atacccatct ccacctcttt gaaacgaaag tttacacgaa
     7981 aaagcaacca aattccccgg aactccgaga taaactttgt ttgtcccgat attgaaaggc
     8041 gcactttcaa tcaggtgcat aaatacgtga tcagacttta cttccaggct gacaagacct
     8101 tgaataatat tgggattatt gacgatggtc agtttgtaaa cctctctttg aggtagttta
     8161 aactcagcct tccagtcgaa attccagccg ttctttttag taatattttt cagttctttc
     8221 gatgttaaaa gcgaaacttc agtctgaaag ctatctcccg actgaacatt ttcaatcgag
     8281 tttgttagtt tatctacaac aaattcaagc cctgccttcg tctgcttttt cataccataa
     8341 agatacaaaa aatgtgttta acaaacagcc attttctctc tctttaacct gccttccaag
     8401 cacatctttt ttaagccttc aacgatcatc cctaatcatt aaaatatgca ccatcatgaa
     8461 gtccgaaaag ctggtggatc gctaaaaaat atagggatca acctccataa atatcggtgg
     8521 aaaggcctca ccttgtcaac agaaacacga actgctcacc ggatatatcc gggaaaatga
     8581 ggtgtagttg cacggtcggt tccttcggct taatgttagc ggtcagttct ttttatcagg
     8641 tcataatgcg tggtaatatt ctttgatttt ggtcgccatt agttttcttc ttaatgtcaa
     8701 aaagtcgttg taatcgtcaa tgctcatatg ttgaatttct gtcggcacac aattcatttt
     8761 caggttatcc aaaagttgtt gttcgtttga aagtccactt actagctggt tgccgtttag
     8821 catttgagtt gtaacgactt caaagtaatc ctttggaggc ttgttgccaa ccttaatatt
     8881 tatctcagac tgcatataaa catagttggc gatttgattg tatttgctcc tgtccaaacc
     8941 gttctttttc aaatagtctt ttggaaacaa gtggtgaatg tcgccacgaa gagaaatcaa
     9001 gtcactaact aaaatatctt ttgataaaaa tcctctgtca ttggctttaa cttgtgaggc
     9061 caggaaaacg tgaaagtatg gactacttgc aaccgaagtg tctaaactct gcggtaatga
     9121 agcgctccaa aaggcgtccg aaagttctgc ttcttctctt tctcccaaaa tttcgtctat
     9181 tggtttgtga gaaatttgtt ttatgtcaaa gtcaaatgca ctttccggtg aacccgaata
     9241 ccggcctgtc aagatagaat aaacgaacca tcgcctaaca aaacgctcta tggcaaccga
     9301 gttaaccccc aactccttta atttgagata aacgatgtag gcaaaattaa ttgcgttctg
     9361 tgaacggata agttttggtg aaataaatcc tgcggattta atgatcatca agaaccgttt
     9421 aaagttagtc tcgttgatga aactatttac accggttttt aaagttgcaa aagattgttc
     9481 tgcaatatcc tcctcatacg tccgtgtttc aaagtttcta cctgaaagca aacttaccag
     9541 gtcagaaagc cgtcctctgt taaactgtga agtaaatgca acgcgaatta aatcgttgta
     9601 gtcggggtcg tataaatcct cgttctccgt tttgagccat tgcagtttct ggaagtaatc
     9661 tgacttggca aattccctgt cgttgtcaac gatgtgtttg aaaaaatcgg gtgcaatgca
     9721 caagtggcag aagtaatcaa tggctttgcg taattcgttt ccgttgtatt ctgtattgga
     9781 agcgattttg ctcattgcaa agtcagcttg actaagcaca acaccttttg aattgatgcg
     9841 tataaatatt tcagtaactg tttctatgtc caggtcagcg gctaactcaa taattccaat
     9901 ttgctttttc ggaatgttca tcaagtttga aaaagcgttg cgaacttgct ttttatcaac
     9961 gtcaggatta agttcaaaat attggtcggc aatttcaaaa aggtcgccat tgatggcttg
    10021 cgaaatatcg ggaagccagg ttctatcttt taagattgcc gggttttgaa cttcaaatct
    10081 ttcatcaatt gggtggaaag cgattttgat ttttaccctc tcgtaggttt tgttgattac
    10141 gtactgtcca agaatggcag ctgtcaaagc tgtcacacgc tgttgtccgt caattaatat
    10201 ttttttgcct tcacttaagc taccatcttt tagccgcacg tttggatttc tccaggcgat
    10261 aacataaccg attggataac cttgatataa actgtccatt aagtcacgaa cttttgaact
    10321 gtcccaaacg aaaggtcttt gaatttccgg aattgctatt tcccctgaat ttacccaagc
    10381 taaaagcgtc tcaatcaatt gttggtttac tgcgtatttc tgcatctgtc gtttttgtat
    10441 ttacttgtct atttgttcgt ggtacagtta ttattctttt caagtcgtct aaatccatag
    10501 taaatcagta ttatgcaaag aacaagtcct gttattgcgg ggataagaat tgattttccg
    10561 gcttgtccgg cattgtaaat gctccataca atttttcctg ttgccataag taccactacg
    10621 ccaagtccca ttaaaaggct acgcttccaa aaggcaagcc caagagcgaa aaacgaaaca
    10681 ggaacggtca atgccaacag tattttctta aaatcccatt gagcactgag ccaacttttt
    10741 tcaaattgca aaaacatact tacctgctcg cttaccgttt cgggtttgct gaaccaaaac
    10801 atgcttgtaa aaagacaaaa aatagttgct gaaactcctg tccaacttcg tttgtaagca
    10861 aaaaaacaaa atggtatcaa aaagacagga cgaataaacc agctcatttg attttggtgt
    10921 cgctcgaaaa cccagtttag aaagggttcg tttgtcattg ctgttacgat aaataccaaa
    10981 gtcaatacaa aaaaaacgag tgcaatggat ttgtcgtgtt ttaataatat ttctttcatt
    11041 gttcgtccat ttagtctatt gatcaaccac aagcttccgg gttgagtttt cgtccgctga
    11101 gatgcaacga acggttctct gcttagcgat gaggctgatt ttaacacaaa cctccacccg
    11161 aagaacggat actcaaaaat agtaaaaact tctaaccgat gcacttcagc cccgattatg
    11221 gcaatagctt gttcctggtt cgttttatct atttttttat aaattttgct gagaatgttg
    11281 tgcccttaat ggttgttggc ttcaatatgt agataccatt tgccaaattg gaaatatcaa
    11341 attgagcgga gttatatgtt gcgattttaa ctccattcat tgaatatagt tctaccgtgg
    11401 aaatttcatc ctgaaactca atatttaatt ctgttgaccc cgggttgggg taaattttta
    11461 ttgagcccgt atttaagtta tcattaatat ccatcgtgtt ggtggtgttc tcgataatgc
    11521 ctaatttttt cagactaaga tcaaaattaa acccatacca aaggatgtca ttatctccgt
    11581 cattgtccat atctgccagg taaatagcat caaacacctc gtcgttgtta atcagttgtt
    11641 ggttttggaa agtgccggtt ccgtcgtttg cctgccaaaa caaagccctt ttatcattaa
    11701 tactcgaata tacagcatcc ggaaaagcat catcatccat gtttgctatg gcaaatggag
    11761 gattctggat gtttgccaca ttgaaggtta cagattgatt taaagcaaat gttcctcctc
    11821 ctgtatttct ataaacatta tattggttag aggaactacc cggaaaaata atatccattt
    11881 gcccatcatt gtcaatgtcg gtatgggtaa caaataaaca aagattaata tccaatgtgt
    11941 taggacttga taaaccaccc atagtgttgt ttagaaacaa ttgtgaacca tccgatgaac
    12001 acaaaccaat gtcaggaaag ttgtctccgt taaaatcggc aacggtcata ttataaaatt
    12061 cagccccatc gagcaatacg attctattgc caaaaacacc tgaaccgttg ttgagataaa
    12121 gtgtgatcat taagtcgctg ttggctacaa tatccaggtc tccatcatta tcaaaatccg
    12181 caaaacaggc tctgattgtt gcaacctcta attctgtatc tatgacggtt ggggttaaac
    12241 cattgttgtt aatcaatact acctgtgaat gatttttgtt gttattggca actaccaggt
    12301 ccattgttga atcgccatcc acatctcccg catccatata aaatggtttt gctatcgttg
    12361 atgtcaggaa taaaggaaca gaaaaggtgt tctctcccag attcctatac catttaattg
    12421 aatcggcatg gtatgaaacc acaatatcct tcaggtcgtc atgatcaata tcaacggcaa
    12481 tgatgtttct gatctgggca gagaagtggc tatcaataac ggttgggctg cccaattgag
    12541 cacttgcact aaaagaaaac aacaagaccg ggatataaaa aataaatatt ttcatactga
    12601 cgaggtttta aatgttgaat attttattct gaggaacaag aggccggggg ttagggtatt
    12661 ggtgatggag gaacaaaaag cacaaagttt caatttatta caaatgtaca taaattgctc
    12721 ttgcatattt acataaagta ttttcctgcc atttaatcag attatagctt tatgaaccca
    12781 tttgaatcag accgaagcct tgggcggttt gctttctaat tcttgttttt cctgttgtga
    12841 tatacccgga ctccatggta aagaatcggt ttgctaattt tatcctattt ttacagtcaa
    12901 agacagagat gaaggcaatc gttattggtg gtacaggagc aacaggggca tatctgttgc
    12961 aggagttgct ggcttccgac aggataactt ccgtgacggc attgaccaga agagcgatat
    13021 cggtcgacca tcccaggctc aatcaacaga ttgtagattt cgaacaattg gaagatgtaa
    13081 gtatttcggg cgatctggca ttctcatgcc ttggcacaac ccgaaaagcc gcaggaagca
    13141 agaaggcgca atggcgggtt gatcacgatt atcagctggc cttcgctcga atggcaagaa
    13201 aggccaatgt acccgttttt acgctgatgt cctccatggg atccgataaa aattcagcgt
    13261 tgttctattc ccggatgaag ggcacactgg aagaagccat catacacttg gactttgacc
    13321 acacttatat attccgaccg gggatgcttg agcgcccggg ttctgacagg ttgatggagc
    13381 ggtgggtggt aagcctcatg aagggcatgc gtcgactggg tatgccccaa aaatacggac
    13441 cctcccatat cagcatcgta gctaaggcaa tggtagcggc aagcctcaac catgatgata
    13501 ccttgaagat gttcgatgtg gcagacataa acacactggc acaagaaaag tattgatgat
    13561 gacaatggaa acagcaagaa agaattatta tatttccggg tatgcggcgc tctctgtctt
    13621 ctttctcgtg ctttattatg taactaattt gccgatattc aaacaatggg aacattatct
    13681 ggcactgatc cagaaattca cccttagtct ttcgcttatt tcggctatct tcctgaccgg
    13741 aaggattatt gagcggctga tcgactcgag gaccgagact gaaggagcgc ggcacaatct
    13801 cttcaggatc acccggctgc taaccattgt cttcgtcctg attgtcgtgg tatcttttct
    13861 ttttcaaaat ctgtatgcgg ttgctatcag tttcggattg atttcgttgg tgctgggttt
    13921 tgcattacag gcaccgacca cctcattcat agcatggctg tttatcattt tccgcaaacc
    13981 atatcaggta ggcgacaggg ttcagatcgg caccctaaga ggtgatgtca tagaaatcag
    14041 ctacctggat acgattttag aagaagtcag cggcacctat ctgggcaatg accggaaaag
    14101 cggtcggctg attcactttc ccaacagcct gattctaacc tcacaggtgg tcaactattc
    14161 cggccctttt gatgatttta tctggaatga aaccgctgtg cagatatcct ttaccagtga
    14221 cctgacattc gtggaatatt gtctgaaaga ggcggctgac aaggatttta tggagcaata
    14281 tcccggcaag cctatggatg acctgcatgc agccgtctat tttcgggtca atacctttgc
    14341 ctggctggag gctgttgttt cttatcccgt agaaccggaa gatacgaccg gcagacgtaa
    14401 caggattctt aaatatggat tggcatcact caatgcacat cccgacaagg taggcttccc
    14461 tgaagggact aagagatagg gtgagtcggg aaagtgaact gggtagataa gtaataactc
    14521 aactgttagc cgggaagggc atttctgaca ttgtcctatt aaaaattggt ttcaattcaa
    14581 ctttttttga tttgctatcc acagcattac agcaataagg caatttgcgg ttattatttt
    14641 cctacctact tcgaaatccc ccctcgccac acattgcccg gaatcagttc gagggcaaat
    14701 catctttcct tattgtagta aataaatgtt gttatattgt ccgggaacaa gagatatcct
    14761 tttcatgtct tttaaaaatt ttatagataa ctgcgataac cggccattta tatcagacat
    14821 atagttcccc ggtatttatt gtttccttcc ggagaggatg tcttgataat tcattcaaat
    14881 tgactaactt tgcatattga tatttaatat atgaaaaata tttacatcat ccggggatta
    14941 tctgtctgat tccctatatt ttatacaaac atttatccaa aaagcatatg tcttccatac
    15001 aagagtttaa cgactatcgg caacgcatga atgaccgaat catggcccag gacaataagg
    15061 ttatcaagcg attctggagc cttgacaatc agacatacca ggcagaagcc ctagatgtca
    15121 ggaccaagga aatgctgggc ctggtggcat ccatggtatt gcggtgcgat gattgcatca
    15181 aataccatct ccagcgctgt catgaggaag gcctgacgac agaagaggtg tttgaagtgc
    15241 tctcagtcgc catcatcgtg ggcggctcca tctgcatacc acacacccga agagcggtgg
    15301 agttctggga tgagttaaac aatcagaaat aaattatggc ttccatttca tttctagatg
    15361 aactcaatga agttcaatac aaagctgtca cgaccatcga tggcccggtc atggtgattg
    15421 caggacccgg ttccggcaag acccgtgtcc ttacctatcg catcgctttt atgatagagc
    15481 aagggatcaa accacaaaac atccttacgc tgacctttac caacaaggca gcacgagaga
    15541 tgaaggaaag gcttgaaaag accgttggcc ccgaagccaa aaaggtttgg gccggcacct
    15601 ttcactctat ctttgccaag atcctgcgct tcgaggctga aaagattggc tttcctgctt
    15661 cttttaccat ttatgataca gatgatacca agtcgatgat cagccatatc gtgaccgaaa
    15721 tgaacctgga tagcaagctt tattcggcca acatgatccg caatagaatc tcttcggcca
    15781 aaagcgctct cctcagcccg actgcctatc ttcaaaacga agagttgatg aatcaggaca
    15841 agctcaataa agtgcctttg atcggaaaaa tctacgagcg ttacgcaaga aggtgcaaag
    15901 aggcgggttc gatggatttc gacgacctgt tgtacaacac ctttgttctt tttcatcgca
    15961 atccggagaa tgtcctggag aaataccgag aaagatttca atacttgctt gtcgacgagt
    16021 ttcaggacac caacttccta cagtatgcca tacttaaaaa gctcgtaaaa tatcccggga
    16081 gcaaagaaaa cctgtgtatt gtcggtgacg atgctcagag tatctatgcc tttcggggtg
    16141 caaccataga caacattctg gatttcaaca atgattttaa ggggctgagg acctttaaac
    16201 tagagcagaa ctatcgctcc ggaaaattca tcgtcaacgc ggccaataat gtcatcaccc
    16261 acaacagaag acagatcaag aaggaaatct ggacttcaca atcggattcc aggagaatcg
    16321 gtctgatccg tgccctgtcg gatgtagagg aaagcagaaa ggtggttgac gccatcttcg
    16381 aacataaaaa tcgcaatcac atcaccaaca gtgaaatcgc tgtcctatac cgtaccaacg
    16441 cacagagcag aatcgtcgaa gaacagttgc gacgagccaa tattccttac cgtgtctatg
    16501 ggggcctttc attctaccaa cgcaaggaaa tcaaagacgt tctggcttat ttcaggttgg
    16561 tggttaatca tcgggatgaa gaagcattcc gtcgaatcat caactatccg aaacgaggat
    16621 tgggtgatac ttcgatcgaa aagttgtttg cctatgccgc agaaaaaggc ttgtcattct
    16681 gggaagccat cgacgatcag cccttaccgg ccaaagccca agctgtgctc aatgaattca
    16741 ggaaaatgat tacaggcctg catcaatttg ctatgaacaa tgatgcggat aaggcggcca
    16801 accacatata tcagaaatcc ggactttccg tggtcctcaa ggcagatact actccggaag
    16861 gaaaggggcg cctcgaaaac atcacctcac tcctcgatgg tatcaaggaa tatgtggaaa
    16921 atgatgagga agtcctcgat gaaaatggtg atttggtgat tgaaaaaggc cgcggtctcg
    16981 tggactatct acaaaacata tccctgatta ctgatttcga tcaggaatcg gacaatggcg
    17041 accatgtcaa tctcatgtcg gtacactccg ccaaaggcct ggaattcgac agtgtgtttg
    17101 ttgtcgggct ggaggaaaat ctcttcccct ccttcctgtc tctgaaatcc gaatctgaaa
    17161 tcgacgaaga acgacgtctg ttctatgtcg ccatcacccg ggcaaggaaa ttgctgacat
    17221 tgtcctatgc caccagccgc taccaatacg gaaaacttgc atacaatgag ccgagtcgct
    17281 ttctggatga agtgggtgaa gacaatctgg tcacaccggt tcaccaggcg agaaatctcg
    17341 tagaggatcg cgcgccacgc gcatctgtat ccggcatttt tactaaaaaa caacccgtaa
    17401 aggccaatgt ggctattcat gacattgtgc ccagccctgt cagcgccttt caggttggcg
    17461 ttaccgtggt acatcctcgt ttcggacaag gcaccatcaa agccatcgat ggcacgtcag
    17521 acaacaaggt ggccatcatc cagtttgata cgggtgatga ttcagataag cgtatcatgc
    17581 tcaaatttgc caagatgcag gtagttggat gatgatccgg cgaatgatac agagcaatgt
    17641 ggaacaggaa atgttcgggt gtgaagtagg tagaagatta atagcgctgt acacgtgatt
    17701 ttagagcctt ttcctcaaag cgggacatca tttcaggcgt atttgtgcag ttgatttaat
    17761 tcggtttccc tcaagtcttg ttgtggcggg actgccgtct attattttcc tacctacttc
    17821 tgtttttcca aaaaactcct tttcgtcccc ctcaagattc gttggtcatc agaaggtcga
    17881 ggtcatcaac aggtccaggt ccgtgtactt gatggcaaat cggtcggaaa gatatttgtt
    17941 ggtgagacag cccttatagg tatagacgcc attgcgcaaa ttgatatggc taaacagtac
    18001 attctcgatg cttcccgcac ttccggcctt ggcaaggatc ggcgtgatca tattgctgat
    18061 ggctacggat gcagtgcggg gtacgttgga ggcgatgttg ggtacacaat agtgtataac
    18121 gccatgtttt tcaaaagtcg gacgatcgag tgacgtcact tcggatgttt caaagcagcc
    18181 gccctgatca atactaacat caatgatcac actgcccggt ttcattcggg cgaccatctc
    18241 ttctgacacg atcaattgtg tccgacctgc cttgctgtgt actgccccaa tagccacatc
    18301 ggcatcggaa agggcctggc caagataggt ggggttgatg gaagaggtga aaagacgctg
    18361 ccctacccgg gcttgtatgc gcatcaattt gtagatattg ttgtcaaata cacagaccga
    18421 agcgcctagt cccaatgccg tgcgcaccgc atactccgct acgacaccgc caccaaggat
    18481 aactaccttg gcaggaggca caccggaaat gccccccaaa aggacgcctg tccctccgga
    18541 tgtcgtggtg aggagattgg cagcggtcag gatggcgctg atgccggcta gctcactcat
    18601 gatccgtaca agtgggaacg aaccgtcatc atccatcata tattccatgg ccagggcaat
    18661 gacccgcttt tgacgcagtt tgttgatgta atcactgttg ataagcggta tttgcaatgg
    18721 tgatatcaga atctgacccg gacgaaagtt gtccaactct tcatgggttg gcggtgcgac
    18781 ttttaggatg acatcgcatt tgaatgcctc ttttgtactt tgtacaatct ctgcacctgc
    18841 ctccgaatat tcatgatcgg agaaattgga acgcatgccc gcacctgttt caacgacgat
    18901 cttatggtta ttaccaacca aataagccac cgatgccgga accagagcga cacgattttc
    18961 catttccatc tgttcccgga tgattcctat cgacagattg gcatgctttt tctttacttc
    19021 cagcatttct gtcttaggaa gatgttcttc aaaataaaca ggatttgaca ctgttgctca
    19081 ttaattgttg tttccaatat tagcctttgg ctggttaaag atacaaattt atgtcaatta
    19141 catattgcga atgaatgaaa tgtaaacaca aaagcattcc tgaaaagctt tcttaaaaca
    19201 accttgtggt agaaataggt taaagtgtca aatgcacgaa ctttcgcagg ccgcatcctt
    19261 caatcggcac agcagaacag atgagaggat gtaaacttcc cttttctatg attccttgaa
    19321 tttacggata gttcaatggt gacaggatga tttatatttg attaaaaata aaattgaaat
    19381 cgtactttta gtatgatact gattgggccg ctttcggatg aaaaccaaat agaacagtgt
    19441 tttcagaaaa ctccctgttg atattcaatt tgccggaaat acttcttttt acccggggtt
    19501 gtttcggcct ccgatatgta ataaaagtgg gcttagtcag accatcgtca gatcggcgcg
    19561 gctgcacact cttttggctt tgttatcctt ctattctgaa tgcttgaaga tggtattacc
    19621 ttccttttag gacattcagt taaatctgaa aattaattta tctctgttta ccgatgattg
    19681 ttatttttga atcgggtttt attcattgcc ttctgaatat tcatttacac aaaagatata
    19741 acacacaagg ccttgtcgga tgttgaggac tgccaatgac atagatagac cgaaggattc
    19801 aataccctta ataactttca aaatatgcat ttgaaaaaca taaccgtttt ctgtggtgcc
    19861 aacagcggtg ccaaccctga attcaccctg atagcccgaa aggtcggctc cctgcttgcc
    19921 gggaggggca taggcgtagt gtatgggggc gccaaaatag gtatcatggg tgctgtagcc
    19981 gatggtgccc ttgcccgggg aggcagagtc accggcatcc ttcctcattt tctcagtgct
    20041 cgtgagattg cacatgatgg tcttaccgaa ttggtcgtcg ttgactcgat gcacgaaagg
    20101 aagttgaaga tgtacgccat gtctgacggc ttcctggtcc tgccgggcgg ctttggcacc
    20161 atggacgaat tgttcgagac gcttacctgg gcacagttgg gtctgcatca aaaaccaatc
    20221 ggcatcctga ataccggcca ctactatgat gagctcatcg cgctggtcga acgcatggca
    20281 gctgaaatga ttctcaagga tatctatcgc tcgatgatga ttgtcggtga tgaaatcgag
    20341 gaacttttaa gcaaaatgga aaattacagg gcaccacaag tagaaaaatg gataacaccc
    20401 gacaaaacct gaaccggtca tgagcataga tttgcaacaa cctctggaaa atgactttgt
    20461 cggccttgta cccctacagg taggcgattt cgaattgctc tatctggtag cctccaatcc
    20521 gaaggtgtgg gagcaacatc ccaacaaaga tcgatggaaa cgagatgtct ttctgacttt
    20581 cttccaggga gctatggaaa gcggtggagc atacagaatt gtcaataaat ccgatggcaa
    20641 aaccataggt agcacacgat tttatggtca tgatccggat gatgagtcca tcatgattgg
    20701 ttataccttc tatgaccccg aatattgggg gaagggttat aatcctgccg ttaagatcct
    20761 gatgatggac tatatcttca gattcgtatc aaaagtgttt ttccatgtcg gtgcggtcaa
    20821 taagcgatcc cggattgcca tcgaaagact tggggccgtc aaggtcggcg aaaaggaaat
    20881 tgaatatttc ggcgaaaaag ctgccgtcaa cattatctat tgcattgaaa aggaaaattt
    20941 cctgtcaagg tatcaccatt aaggtagtta aaggacgcct tccggaaatt gcatggtaat
    21001 gcaatgcagt gaaccatgtt gcaggatcag tgaattacaa tccaccggat gaattttcct
    21061 gttcgggaaa catttttgta ttactcttac cgccatgtcg tcctgtttcg gatcgccata
    21121 tactggtaaa agtacggcac cattgatgat caggaagttg gcataggtcg caggaagcct
    21181 gtacccttcc ggatgaaaac gggcaggggc cataggcaat ggaacgaggt tataagggct
    21241 gccatcggaa cgggcaaatg tcttcagctg gcactccata gccttcaatg actcatagtg
    21301 ttcatcttcc ttatcatcac acgacacata gacaatggta tcttctgaac agaatctggc
    21361 gagcgtgtcg atatgtgagt cggtatcatc accggccaga taaccatggt cgagccataa
    21421 gaatctattg acaccaagaa tttccctcag ggtattttct gtcatctcac ggttcagatg
    21481 aggattccgg ttagggctta ggagacattc tgaggtggtc aggagagtgc ctgtcccatc
    21541 gctctccaaa gctccgcctt cgatggccag tccataggta tgcactgcca ggtcgccaaa
    21601 tattcctttg gcctgcaatc ttttggtaat gaggttgtcg tgattggccg gaaacttcag
    21661 accccagcca ttaaagatga agtcaagcaa ggaaggatgc ccctcctcca ggatggtaat
    21721 cgcactgtga tccctggccc aggtatcatt ggagggaagt tcgacaagta taagattctc
    21781 ttgaaccgca tctttcaaca gggtgctgac caactcggca tcctggcata caacaaggac
    21841 tttttcatgc cgggagacca gcgtgatgat ctcaacaaat gtcgggatta ctatttccag
    21901 atatggcccc caatcactgt tggcatgagg gaaggtaaat gccacgccac tttgacgtgc
    21961 ccattcagca ggaaatattc tttgtcccat ttattacgat tattgccaca aaaataggaa
    22021 tatgcagcga agtgcaatga atttaaagga tttctaagca cacaataaca atgaggaatt
    22081 atatctacca catccacccc atgcccacca caatacgggt taagtttaac acaccgcttg
    22141 acaatactag cttttccttc cctttatctt attatttcaa cgaaattctt gatcacataa
    22201 tccttgccat cgttggtttt caaagacaat acataataat aggtcccttc cggaagtggc
    22261 gaaccgtcat tattttttcc atcccattca ttgccgtatt ctgagttttc atatacagca
    22321 ttaccccagc gattgaatat tctgattgcc attcgtgtaa cattgcactt gggaaaattc
    22381 ggtattatca acacatcatt tctgccatcg ccattcggag ttatggtatt gacaaaacca
    22441 ccgttgtcgt ccgtacaagg aaccttctgc aatagatata taattcctgt atcgcatttg
    22501 accgggcaat ccgtctcaca caacaggtat ggcatcttta tcaattccgg gtaaagattt
    22561 tgtcgggctc catcaaaata aaattccatt cgctggtccc ccacctcacg gttaaaaagc
    22621 ttgcctatct cattcccatc atcatctgta atgatttctg ataatggtct gactgaataa
    22681 tctgccggat tggttatact atcgttagat atgacatcaa taatcagcgg attgtactgg
    22741 aaatgccatt gcaggtaatc atcgactgct gaaaagtccg ccttgataaa aggcgtaatt
    22801 gtcaactcag tataacagag atccacatct accactgaga tggtgtatac atccggtgtt
    22861 aaacctgtaa agactggatt ttctccatat actccatggt tcagggcata ggcatatgga
    22921 ggccttcctc cttcggctgt aactgaaatg atgcccgtgg cactgttgca atcaaagccc
    22981 gaatctgtca gattagccgt aaacggatta ctctccagaa gggttatcgt agtatctttg
    23041 gtacaaccgg cattatcaac gacactcaac atataactgc cagcactcaa attgtccatt
    23101 gaaaccggat aagagaccat gctgccattg actgtgaagg tatacggcgg tcgccctcct
    23161 gtaacataag cgctgaaact accgttgtga tctccacaaa atgaatgctt cactgaccca
    23221 aggataagac ttaaggtatt accggcatcg tggacctgtg tgacagaggc tgattgacat
    23281 ccggcagcgt cgatgactga aaccttatat gttccagggc ctaaatgatc gaaaattact
    23341 gtatcggtac tgacatggtc gatggtcttg ttctttgccc ctgacaatga acagctatat
    23401 ggataggtag cattactcac cttgacatgg atcaggccat tgttctgtcc gcaatatgcc
    23461 ccttccgttg acagaacaat gtctggtgcg catgggcaat cgactgctgt gattgccagg
    23521 tcattggcac aaccctccga tgttgtccaa cgaaggatat aacttcccgg tccggaaaag
    23581 gatgtcacca gcgtatttgc tgatgtaggc tcagatatac tgacagttag acctgctggc
    23641 gttgttgaaa ccagattcca gttgcccgaa ccggaagcag ataggataac tcctccggat
    23701 tttgtacaga cctccgccac atcggaagaa acatgaggaa tgagatgata ttgtagcaca
    23761 aatttcatgg tatccgcata gcatgggcca ttcatgtccg ggtcatcagt atatagggtc
    23821 agggtgatgg ttttgccggt atcagcttgt gtcggagtat aactgaaggt aaatgtattt
    23881 gaagaagtgt gagtaaccga ggcattacca cttccattgt ggctgacaag taccgtagtc
    23941 gcattgaaat atgtgttgtt ggggacggag atggttttca ggtcacaagt ggtgaatgat
    24001 gttgcactga gattcagatc gggctgagcc gtacagtcac aatcattctt tgcctcgact
    24061 gtgagggtat cactacaccc tgaggttaac tgtctgataa gagtataata tccggtgccg
    24121 gagaagttct ttatggtagt cttgacattt gaggggtcga ctatgtcaag tgtcatgcct
    24181 ggtggatcgg tatcgataag aatccaaaga ccttctccac tcgtggcact gatgatggca
    24241 ctgtcccggg aagcgcagtc aacctgtata tcaggtccga cttcgggatt tccgtcaatg
    24301 tgcagaaaaa aaatggcagt cgcaggctca cagggaccgc ttccatcatt gtcgtctgtc
    24361 acgatggtga tttgaatggt tttaccaaaa tctgaagggg tgggtgtgta agtaaacatg
    24421 aatgaattgc cgggaaagac aagatgttcc agcgttccgc ttccgttgtg aaagaaagaa
    24481 agggtactgg catttaaaaa gatattgctg tctaccgtta tggcttgagc cccgcaaaca
    24541 ctcttttcat caatgcttaa tattatttcc ggcgggtttt cacaagggca gatatcctgt
    24601 gccgtgatga tagcctcgtc aaaacacccg aattcattgg tccatcttaa tatataggtc
    24661 ccggggccgg aaaagttgtt aacccctgta ttgggatcat tcggatcaat aatatttaag
    24721 ttcagacccg gcggatcagt agttagaagt tcccattgtc ctgtgcctga tgctgccata
    24781 taggcgtatt ctaggtgcca gcaaagtata ttctgatcga taccggcata ggcctttggg
    24841 taaacagtaa agccgatctg gtcaaaacag ccgttgagcg cagtataggt aatgataacc
    24901 gtgcccggaa catttcccgt gacgagtcca ttattgtcca ccgttgcgac atcaggattt
    24961 gatgaggtcc agggattact ttgggctggc ataccactac ctgtaagttg ttcttgattc
    25021 ccttcacaca cccaattatt accactgatg tctggacttg gatagatggt gatggtaaat
    25081 gtttttggac tgccggagca gctggtattg gatggggtga cggtgatggt ggctgtctgt
    25141 gtaccgttta catttgacgc cacgaagttg atattgccac tgccggagga gccaagccct
    25201 atactggtat tggaattggt ccaggaatac gaagtggcat tacttccgct gaaaataacc
    25261 gttacttgtg tgcccccaca ctcaaagatg ttggagggtt ggtcgaccgt gggtagaggt
    25321 acccgggtga tagtcccgct taccgatgtg cctgaaggcc cggtaaagcc aatccaggaa
    25381 caagaaaccg tataattaat caagtcaacg gttggtgttc caaaaatagt caactggctg
    25441 ttattttgag aaaatgacgt ttgcaaccct gttcctgata agccatccac attgaaggag
    25501 ccggtgatat taattatgat aggaattata gactcccctt cacaaacagt ctggtttaaa
    25561 agatccgggt tgaccgcatc aacagagcac tgggaataga cttctgttgc cgaccatatt
    25621 agaagtacac ttaaaagtat gtttttcata gtggtgaatt ttgaatgatt tatgccaaat
    25681 caatgacctc aaaagacaac ttgttactaa tcattagttt tccggtctta caattcgttc
    25741 gttatccaat ccttgcaggt tacatacttt cttgtcttcc tacgagccgg agccgctctt
    25801 ccttttatga agcctgccaa actagtgatg cctatgggtg ataaaacccc tatccacatg
    25861 ctacgatttg tgtttacaaa tttagtgaaa tctcgataaa aaagcagatt aattatctga
    25921 aatgtttcag tcttaggatg ttaataccaa aaatttatgg actattatat aaatcatctt
    25981 ggtataattt cttcaactcg gttccagatc ctgcaaatag accgtatttt actgttatga
    26041 cactgagtcg tcgacttttg aacaggctac tctgatccta tctttttgtt ggtcctgtgt
    26101 ttcctaggat ggttttaaac atttacctta atcaggatta gtaatcaggg cttcataggt
    26161 ttaacggcca aaggaggtaa tgaaggattc ttgtgtgact tgattggagg agagagtggc
    26221 cgggatgggc tgagctggaa atccctggct tttacaccat acaacaaata aatattttta
    26281 tccaattacc ttagggtaat cataaaatat gggagtgtgt gcttcccgga cttttgttac
    26341 tgaatcctct ttggtctatc cctaaggacc aaagaaattt gacttgcaga tagcattgat
    26401 ttattggcaa cattcaaaaa caataagcgc aaaatcgccc tcctgtctta tctgtccctg
    26461 tataaattaa aaaaccaaac atcagcgcct ctgagaaagc catgggttta ttttaatgat
    26521 caaaacgcgc tggtcttcaa agaagcctcg cctgctctca acaccttaat gcctctttct
    26581 cccctctatc agtttccttg tttactcgga aattaatgtt ttagtatctt tgtcgggcaa
    26641 ccaaatcaat atttgaccta tcgaactaaa tttcaagcaa tacggcgaca gtggcccgga
    26701 tgttatcatc ctgcacggtc ttttcggcac tcttgataat tggcagacca ttgcccggag
    26761 gttgagtgac cggatgcggg tcaccacctt tgatttgagg aatcacgggc gttcggcgca
    26821 tgttcctgat ttttcgattg agctgatggc ttcggatgtg tatgagatgc tgcaagccct
    26881 gcatattggt tctgccgtca tcatagggca ttccatgggc gcgagggtag ccatgtgttt
    26941 cagccttgcg tatccggcag tgaccgatgg cttgattgct gtggatatgg caaccaaaaa
    27001 atacgaaagg ggacatgacg acatctttgc cgcattagag gctgttgatt tgaacgccgg
    27061 gtccaggaaa gaggtagaaa acattttagc aaaacgtatt cctgacgagg gcactcttca
    27121 attcctgatg aaaaatttga gccgaagaga aggaggtatc ggctttgaat ggaaaatgaa
    27181 cctgccggta attaaagcta attacgaaac catcctccag ccaatagcat cagaaatagt
    27241 ctatcaggga ccggtattgt tcattcgcgg tggcaacagc aattacgtcg ctgatgaaga
    27301 tttggcagat atcaaaaagt tttttcccta tgccgaactg gcaacagtcg ccggtgccgg
    27361 acattgggta catgctgaaa ggccggatgc catccttaca ttgatagaac aattcgttgc
    27421 agaattataa tcctatttca aagatcctta attcctgtaa tacttttcga accagatatc
    27481 gtaaagttca gcggacctta accgcagcaa ttccctgtaa ttggcattgc tgatggcata
    27541 catatcgtat gggaagcgtg ccgaaatgaa gtcccccttt ttattgagtg cctgattgta
    27601 atattccatc gctttgatct tgttgtcaag tgtcctcact aacaagacct gcctgtcctg
    27661 attgccatcc ttaagcgtga tcgtggctat cttgaggttg gcgctgctga aatatcgcgt
    27721 attataatcg gttacctggg ctctaacctc ttctgttccg gtaatttccg gcttgaaaat
    27781 gatcaggatg aagtgttgat ctgcatcggt agccttgtat atcttttcgg cttcggccag
    27841 ttgttgctcg ttggcatttg agccggtacc caggagtctg agcatttcct tggctctttt
    27901 ctcttccatg gtattcggat aggcttgcac caaatccttc aatgcggaga tataggtttc
    27961 tttccctttc aggtatccca gagacatggc cgtgaccatt gcaaacttag gtcgaaggat
    28021 gtttttgatg ccgaagatgc tgtcgctttt ttgtgccata ttgaaagctg tggcataatc
    28081 tcctttttcc atggaggtgt acgtggaaag gtagtagtca ttgagctctg cctccttatc
    28141 ggctttattt ttaatatact gcgggtcgga gaaataaagc gaatattgat tcccgttgta
    28201 tttggtcttc agcaggtcgg catagtaatt tgctttttga gagttgccct tcatcatata
    28261 agcaaagtac aactgataga aagcctgtgg ttcatgtggg ttgtccggaa accgctgtag
    28321 caggtcttcc agcacctcta ttgctttatc atagcgctcg attttatccg gataaagcac
    28381 gcccagggca ttcatggcat ccataattgc tgcttcggaa cgagcaatcg ttgcagcatc
    28441 ggtcggcacg tcagcaagta ccttctttat ctctgacttg gaaatgaata aggcagagag
    28501 accttcttcc ttatcggtgc ttgtgttgtt gtcaaaactc tgaagtaatg ccgaatacct
    28561 ccagttgtcg gccagaggtc tgttgttcca gtctttgtca aactgtcttt tgccgtcctt
    28621 tagcgtctta tcattatagg caaagaaggc tgtcggcttg gcgccaccga tttggcccgg
    28681 attgccgaat gcagggccgc ctccaatagc agccggctga gagccgcctc tgcctacttt
    28741 agggttgaat gtcgagcctg tattggatgc ggtggcaagc ttttcctttt cacgtgcttc
    28801 cttgatgagt ttcttggcaa gagcctcctg ttcttccggt gtcatcctgc ccaccatcaa
    28861 caggctgtcc tgatactgga ttgtttcaat attataggct atatccttca ggttgtccct
    28921 cctgtcaacg atctccttga atcgttcctt tgttttcgga taggcagttg ctgtggagtc
    28981 ataataatga tatgctaaat caaattgatc ccgtttatag aaaatatcgg ccagcttgag
    29041 gtagctctcg gccttggcct ccggaccttt cttgctgacc tggatacctt tctgcaggtt
    29101 ttttattccg gctgcgatat cgttattgcg tagattgatg tctgccagca tgaaataaat
    29161 ctgatcttgg tattcgtcgt ttttaaggtc tttcaacatc ttattcagcc tggaattgag
    29221 ttcttcatca gatatctttc ccgtagcgac cgaacaccca aatttgctca aagaagcatt
    29281 gaatgccatt tcgtaggagg gcttaagccg ctcgacccgc tcaaaagcct gagtagccat
    29341 atcataattg cctgccttta tatacaactg tcccaggata tacgtcagtc gtgcccggcg
    29401 ttttttctct ttgaccatcg ggatggcctt gttcagattt tctatagcct gcgtataact
    29461 ttcctttttc atatccacct gggcagaaac caacaatgct tcccgccgta ctaagtcata
    29521 cgtaccgcct tcattgagca gcgaccggag gattctctct ccatcctcat aattacttcg
    29581 ttgaatgtag gtcttcgcca accaaagctg tgcctgttgg aatatcgggc ggtgtttgaa
    29641 aaggtagttt ttaggtctga tggaaggctt gtccttttta gttgctacca cattgtcttc
    29701 atcttcctta acggacttta ccggttgtgt atctgctttt tccgtcttca cttccgtctt
    29761 gttgtcagtc ggcttgctat ccggagttgt tgtgcctgcg ggctttgatt ccggtgcttt
    29821 ggtggttttc ggggcggttt ttggggtgga tttgcccttc ttcttagctg ctgctgcttt
    29881 tttcctttcc ttggctttct tttgggccgc tttttttctt tctttggctg ccttcttacg
    29941 gtccgccgcc ttttgcttgg cttccttctc ctttgctttt ttagcatcgg ccttttcttc
    30001 ccgaactttc tccttttcgg cttccttctc tttttttact tcctgcctct ccttttcaat
    30061 ggcttttttc ttcttgcccg gcgacattct gttggctttg tcacgagcaa gctgtgcttc
    30121 actataattc tgaacaacat atttaaaggc gttttctgct ctttcataat cctttttcag
    30181 gaacagggcc tttcccatct gaaaataact gtcgtccacc caatgactgg caggatgaag
    30241 gtaaatatct ttcttgagtt tctcaatggc agcattcagg gttcctgaag cggttgtgga
    30301 gtcctgatcc agcaaatacg ggtagataat caattgttcc tgaaaattgt ccggatgctt
    30361 gctttcgatt tccagcatgg ccgcatccaa caattcattg gcattgaaat acccgttgta
    30421 gtatgcagtc atgttctgat aggcacgacc caacaagccg acatcttcct ttttcttatg
    30481 tggagaggca caactgttga atatcagcgc tatgacgagt aaatacagga gtgccgattg
    30541 atgtttcttc aatgtatatg ttccttttgt tataatgttt tataagtcag tagacttaat
    30601 aacgttattt gtctgaaaat tcgcacaaag ttacatcaaa atttttacaa attggacaga
    30661 ttggcggata tgagttgtcg ccaaacaggt aaaaaagaca cctgcattgt tatccatagg
    30721 atgatgtcta tcctgtaata tccttcaaaa cccgacggag gtttcttact taacctcctc
    30781 aaagtgaatt tcttcgtcct tatatgccga agtcctctgc tgacggggat tacaatatgc
    30841 cgcggagcaa catcccacat tcaacaatgg catcaaggta aatatagctc caaaaatcag
    30901 gaaaaacacc tctccacttc gaataccttc gaccaggata atggctccga gaacaagtcg
    30961 caatatgcgt aaaaaattcc agttgttatg catggcataa tgttatatgg ataccgggga
    31021 tattattatc aatcggtaat tatccatata taacggatat tatgccgatt ggtttaatgg
    31081 ctgatcctgc ctatttctgc tcctttgcct tacacgtgtt gattccgaaa atctggtaaa
    31141 tgggacaaaa acttatatag gatgtcaatg cgaatacaat ggcaaagaaa agtagcagca
    31201 aggccatatt gcctgaaata gcatttgtta gatataaagc aaggatcaaa actgccaata
    31261 gcaagcgaat aaatttatcg ccggttccta aatttttttt catgttttct atttcattaa
    31321 atgattgata aaattaaatc attttttcaa atgacaagtc atattgtggc aacaaatgga
    31381 aaatattgtc ttgcagcacg aatcattcat gccgccaaat gagtatttga tgggattggg
    31441 tccccgatac ccgccctttc ttaatccttg aatattgcta aatgacatgg tcaaaggtgt
    31501 actttatttc atatttttat actaagttta ctgaaaaata cttcttattt catgttatta
    31561 aaaatactcc aaatgaacct catcatcaga tttttttcgg tatagccatg attgctcttg
    31621 ctcacatggt caatggccaa tctgtcaaac cgatacatac cgtccatggc agttttaccc
    31681 cacctcctaa agaggttgtc cttcaaaatc atgaaagccg caacccttcg cgtgaaaaaa
    31741 ttgtatggat acaacttgcg gatgatacca taaatggttt tcgggattcg attgtcaatc
    31801 ggttgagtcc tgtggatttt atcgatgaca ggacgtttgc tgtgagggtc gatgcagtcg
    31861 gattgaaaga tcttgctgca aatgaggctg tagtgtcgat ctttgaaatg ccggcagcat
    31921 taaaactgga ccgtaaggtg gtggatgcgg atgactccga aaaactggag gttaggatca
    31981 gcttatatgg agctatgtct gattcagaga ttgaaaatct gatcggaccg gtaaaaataa
    32041 tctctcatca tgtttccggt gacaaaactt acttggtgat tgcctgtgac cggccatctg
    32101 ccttggagat agctgccggt gctcaagtca gagccatctc cgaaaatata tctgcattta
    32161 cacccctcaa cttctacaag cgctcactgg aagcagtaca aggagcaaat gcatccttgc
    32221 aaaatggtgg attgggacta aatggcgccg gtatcacaat cggtcatggt gacacagggg
    32281 ggtttttcca tgaagacctg aatgacaggg tagtccattt cgctctggat tcagtgaccg
    32341 cccatgctcc tcatactgca ggaagcatga tcggggcggc taacctcaag gataaacacc
    32401 gtggcatagc acaggaagca acactggtca atgccactta ttttgatatc atctcgtacg
    32461 acaatcaatt ttacaatgac ttcaatgtcc gcgtaaccaa taattcatat gcggtgctgg
    32521 ataagaatat cgattgcaat tcaatgggcg aatacaatat attcagtgaa gaggcggatc
    32581 aaagtaccta tgagtatcag gaccttttgc acgtgtgggc gtcgggcaac agtggcacgc
    32641 ttgcctgtgg tgaccatatc gccggttatg gaactgtcct ttcagggccg caatgcaaca
    32701 agaacggtat caccgtaggc gccatctgga ggctggaggg tacacctctt gccgattttt
    32761 caagcaaggg tccgacaaga gacggcagaa tcaaaccgga aattgtggcc caggggacta
    32821 atgttaccag ctgcaatggc aaaaatgact attacaccac agccggtacc agtatggctg
    32881 ccccgggggt cacaggagcc attgctttga tgcaggaagc atacaaaaag gccaacaacg
    32941 gaaaatatcc atcagcggca ctgttgaagg ctattctaat caatacgtgt gatgaactgc
    33001 tgggagaagg tcctgaattt tctgtcggat atggcaaggt gaataccatg aaggctgcaa
    33061 gagaggccgg taaagatcat catgatatcg gaagtgcctt ttcagggagt gaagatatat
    33121 accactttga tattcccgag ggtcaggcta aggctgtctt ttcactggcc tggactgatc
    33181 cgcctgccaa cccgctttca acccgccaac tgatcaacga cctcgatcta atcgttgtcg
    33241 gccctaccgg ccaatatctc ccatgggtac tcaaccccaa tccggactat gtattcaacg
    33301 cggctgtgcg gggagtggat agcctcaaca atctcgaaca ggtcagtatc ctgaatccgg
    33361 tcgccggaaa gtatgaaatc atcattcgcg gcaaaagggt cgcaggcagc caggaatatg
    33421 cgttatgtta tcgctatgaa gctaaaaaaa tggaactgta cggaccattt gggggtgaaa
    33481 gatacatccc gggcgagttt caatactttc aatggggcag cccatacaat accggagact
    33541 ccacagtgat ttcgctctcc ctggatgatg gacagacctg gacagaactc ggccgccgtg
    33601 cagagaatat ccggacattt aattacatca tgcctaatgt gacttccgac aaagcccggt
    33661 tgatgatcca ttccgacaaa atgaccggcg acacttcggg aagattcacc atttgtcctt
    33721 caccaaaatt taattatatc tctacctgtc ggggtgaagc tgatttgagc tggccaacgg
    33781 tagatggagc aagcgcctat aacatatatc ggaaaacggg cgcgtacatg gagattgtcg
    33841 ccactactac cgacacaaca tacaggcttg ccggtcttaa tccggacatc gatgagatga
    33901 tcagtgtcgc agctgtgttg ccggatggcg gcgttagtcg acgtgatcat acccagtttg
    33961 tcaaaactct tggtgttcaa tgtgatcaca aatttgatct tgaaattctt gaaattacat
    34021 caccggagag attcggcagg gagttgacat ccacagcatt gaccaatgag gagaaggtgt
    34081 ccataaggat agttaacaaa ggcctggaag cggctcaggg attcagtata cactatgtca
    34141 tcgacaacat cgaaacggtg gaagtattca atgaattgat tctccccgga gaaacagccg
    34201 aatatacctt tcaacaacct gcgagattat ctgaagccgg aaatcatcaa ctccgggtgt
    34261 ggatagacta caactttgat tcaccctatc attcggacaa tgcaaaagag atactcatca
    34321 ggcagattcc gaacaaggag attgttttaa gccgggagct aagttcaact tttcaccttg
    34381 gattcgatga ccagcatacc ggtgtggctc gcaatgggga caaaggcatc ctcggcgtgg
    34441 atgaaattga ttttcattcc aacaatccac gcggaagggc ccgaatggta gtgccgtcag
    34501 ggttcaacaa gtcgggaaaa ggtgccctga ctatggatgt cgattatcag ggaacatccg
    34561 tgcagaatga agcaatattt accttcaacc taagcaaata taccttggac gatgatatca
    34621 atcttgaatt atcctatctg aatcaaaatt caccgctgaa agtagatagt ttgacggcca
    34681 agttgtggat tcggggaagt gacagtgata tctgggttga agcatataac ctaaaggaaa
    34741 atgaggcccg ggccggaacc tatacttcaa tccgaagttt gagattggat aaattgctgc
    34801 gggatgccaa ccagcagttt tccacaagta cacagttgaa aataagccag cttgtggatg
    34861 gacctgctgt ggcaagagat ggtttttcgg ggtatacttt cgatgatctg tatctctatc
    34921 agtctcaaat ggatatgcag gtgaccagaa tcatcagtcc cgcacccaat tcctgtggcc
    34981 tcactgccga tgaagaaata accctggaaa tagcaaacac ttcgaatgag ccggtggatt
    35041 ccttatcgct atggtacagt gttgacggtg tggttaccgg accgatcgac attccaccga
    35101 tgcttggcac cgaggtgagg acaataagcg gcttgccggc agatctgtca acaataggcg
    35161 ctcacaccat taaggtttgg gttgccgcac cgggagatac ttaccgggct aatgatacgc
    35221 tttcgggata taccgtttat aacaatggct tcgtcgggca attcccctat ctggctacct
    35281 tcgaggcagg caatgaaacc tggatttctt ccggatttca atcatcctgg atacgtgcaa
    35341 caccggacaa agacaatctg caaggcgcag ccaatggtga ttttgccatg gttacctcgt
    35401 ccgatggcag gtacaatggc tatgagcgtt ctacgcttac atcaccgtgt tttgacctgt
    35461 ccggcctgca ggaaccctat ctttcttttt ctttctggca tgatacagag gtggaatatg
    35521 atagttgctg ggtggaatat agtgtggatg gaagtgcctg gaaaatactg gaaaatacac
    35581 atagaagtat taattggtac aacagaccat tcgcctggga tggtcaggat gatcactggc
    35641 atgtggcggg cttgcctctt ccggtacagc tctttaccga tcggtccggg attcaattca
    35701 ggtttgtcct ctatgccgat gaaaatgtac agaaaggagg aatggccatc gatgacattc
    35761 atatttatga gggacctgat atctacgtcg gcggtgataa caaggtcagt tttctggcaa
    35821 atcagcactg gcaagacatt gtcgtcgacg gcagaaccat cgccgccatc aagtcagaag
    35881 acccgcagcc tgaacctttt acagtgggtg tacatttcaa taccggaacc gaacaaaggc
    35941 atgatcagtc gcaatattat cttgacagga gcctttccgt caataccgcc tccggcactg
    36001 caaagcctta caacatccga ttttatttta ctgatgaaga agctgagcgc atcattcatg
    36061 ccggtccggg ctatcatgcg cctaactccg cataccaact gggcctcact tcaatatcat
    36121 cagaggataa tgatggggtt tttggcaatg aagtgaatcc ggaatatgat ttctttagtt
    36181 ttaaaagaac tcaactggtg ccttataagg acggttatta ccttgaattc agggcaaagg
    36241 ggcccggcga atttttcctc aattccgggg gcaaggaatt caatcttccg ctgggcaaca
    36301 caacaagcaa cgtctttggt gttggtttgg ccggggacaa tttgactgct cgcccgaatc
    36361 cattcagtga tgaatttgtc atttatctgg agacagcgca aaagagtaaa atatataggg
    36421 tagaattggt ggatgtatcg tccagaatag tcaataccta tatggtgccc ggagagtcct
    36481 tgtcggaagg gtttcctgtc aggactcgtc tcccggcagg cgtgtacctg atacggatac
    36541 acggaagtga tctccatgca gtgagtcgcc tggtcagggt ggactgaagg caggcaattg
    36601 tatgaccggc ccccaatcca caactaatag cctgcaggtt tgttatatcg gacaatcaac
    36661 tgtggcttgc cccgggcgag gggagataca gtctgtttat aacggtctgt catcctgttg
    36721 tttgtaatta ttctgatttc ttttcatgtt gaattgccat atctttgcat catgaagcgt
    36781 gttgtcgtag gattatcggg aggtgttgac tccagtgtcg ctgcctacct gctcaaggag
    36841 cagggatatg aggtaatagg catgtttatg cgcaactggc acaatgatga ggtcatcctt
    36901 tccaatgaat gttgttgggt agaggattca aacgacgcac tgctggttgc ggataagctc
    36961 ggaattcctt attatgtgat cgatctgagt gctgaatata agacgcgcat cgtggactac
    37021 atgtttgcgg agtatgaaaa aggacgaacg cccaatccgg atgtgctgtg caatcgggag
    37081 atcaagtttg atgtatttct ggaagcagct atgaagctcg acgccgactt tgtggcgacc
    37141 ggtcattatt gccgaaagac aaccgacgct aacggccgtc atcatttgct ggcaggaaaa
    37201 gatggtaata aagaccaatc ctattttctg tgtcaactca atcaggacca gctcgccaag
    37261 gcattattcc ctatcggcga cttgctgaag tcagaagtgc gtgaaattgc acgacgtgaa
    37321 ggtcttgtaa ctgccgagaa aaaggattca caaggccttt gtttcatcgg caaggtggat
    37381 ctgcctactt tcctccagca acaactacaa cccaggaagg gcgatatcat cgagattcct
    37441 caggagttgg aacgcctggt gcagtaccat gagattccgg tatctccgga aaacatagag
    37501 gccctttctt ctccatttac ctttgaagca acggagggta tcaaagtggg cgagcaccgc
    37561 ggcgcccatt attacaccat cggacagcgt aagggtctgc acatcggtgg ccggccgtat
    37621 ccatccttcg ttttacagac tgacaccagg cataatatcg tcttttccgg ccaacaaagt
    37681 gaccatcccg gactcaatag gtatgcattg aagattgagg cagatgaaat tcattgggtc
    37741 aacccggact atgagttatc cccggactca tccatagaaa tggaagtacg catccgctat
    37801 cgacaacctc ttcagaaagc taccctcatc aggaaacccg atgcattata tattttgttt
    37861 gaggagatgc aaaaagggat tgcgccgggt caatttgccg cgtggtatct aggagaagaa
    37921 ctcatcgggt ccggagtcat tgcctgagtt ttcggcagga aaaaatatta gtcacccgaa
    37981 attctccaag atgagacact ggtgtagggt gttaagcgtg gtctttttgt ctcattttcc
    38041 tgacgttggt attacccggg atatagcttt ttcttttccc tccgtatatt tcgaggtaag
    38101 gaaaaaaccg gctctctaca atcggcctta tctgtcaaaa taaaacattg cataagttgc
    38161 ttcgagcgac atcagacccc taaaagggac ggccgcccat caatgaagct accttcatct
    38221 tactaaagat ctccgttatg ctccggcagt gtaaccatca aacgatcaac gcctaatacc
    38281 aaaaatttat ggacaattat ataaatcatc ttggtaaaac attcactaat cacccgttaa
    38341 taatgtcaat gccgcattta attcatctta gcatttgaat ttggatgtcc tcgctaagca
    38401 actcatttgt agctcaggat ttccaatcaa ttcatgcact tttcactttt tgctttattt
    38461 ctttggtaca gcggcctgat gcctcctgat gtacttgcca gcatttattc ctgatgcgcc
    38521 aaaatgttat aaatacaaat gatttactgt aatgtcggtt gaatttctat ttttgcacat
    38581 taatccaaaa agctgcaagt gaacacgaag accaagcttt tagccatcat attcacggta
    38641 ctgatcatcg atcaggcact gaagatatat attaagaccc attttcagta tggtgaggat
    38701 atgcccttat ttgggagtac ctgggcatat ttcaatttcg tagaaaataa gggaatggcg
    38761 ttcggactga gcctgggggg agattatggc aaactggcct tgagtttgtt ccgtattgtt
    38821 gccgtcattt ttctatggat atacataagt cgcctgataa aagcaaaggc ccacatgggt
    38881 ctgctggtat cctttgccct catctttgca ggtgctgtgg gtaatattat cgatagtgcc
    38941 ttttacggtc ttattttttc tgaatcatca taccacggtg gggttgctac catgtttccc
    39001 gagggaggtg gttatgccgg gtttttgcat ggcaaagtcg tagatatgtt ttatttcccg
    39061 atcatagata cggtgttgcc tgattggatc cccttctggg gtggacagag atttgagttt
    39121 ttcaaaccgg tattcaatgt tgcggataca tccatcagtg tcggtgtcgg tattcttctc
    39181 gtcttctaca aaagattctt tggcgagcat gaagagactg ctacaaccga cgtttcaaac
    39241 aaagaataaa gctggtggag aagccctgtt ttccgggtat tttgctctta ccccttacat
    39301 tggatgtata caaccgtcta agtacatctt ttttcatgtt atcggtggtc ataccttagt
    39361 gggaaaataa gaaccatgac aaagtatgag aaaatggcag atcgatttgc gaagtccggt
    39421 cttgataagg atgtttttgc aatagaagca gggagaaaag gatcaacaat gatggagccg
    39481 tcagagccgt tatgttgtat tcttttttta ttatacatgc aaagctaaag gaattaacac
    39541 ctatgaatgg ctcgtagata ccctcaataa aactctatcc tatcctatca accgaataca
    39601 tgaactgctc cggggatata aagaaatgat gggtagttgc tcggtcgatt acgtttgtct
    39661 gaatgtaagc aaccgtctaa gtacatcttt tttcaagtca tctgtggtca taccttag
//
LOCUS       DAOQMC010000002        28478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_530_length_28478_cov_5.406713, whole genome shotgun sequence.
ACCESSION   DAOQMC010000002 DAOQMC010000000
VERSION     DAOQMC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 28478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28478
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_530_length_28478_cov_5.406713"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKV31_00145"
     CDS             <1..312
                     /locus_tag="PKV31_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91284.1"
                     /translation="GYEEALFNYLTPEHLDQFSSSELNSYAWYVYESTEGADKAKAAI
                     PWAEASVAKKSEYANNDTLAWLYFKAGDVERAKEIARKAIELGKAAGEDTSSTEELLQ
                     K"
     gene            761..1810
                     /locus_tag="PKV31_00150"
     CDS             761..1810
                     /locus_tag="PKV31_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNB91285.1"
                     /translation="MHLHNRVNGRELKARMRQSDEKRITISFYKYHKIENPQFLRDHL
                     YLEWAKLGVLGRIYLANEGINGQISVPEESHEAFKAAFEQLDFMRGIRLNYAVDDDGK
                     SFFKLKILVRKKIVADGLNDSTFDVTNSGIHLSAKEMNELLEQSNTVVVDMRNHYESE
                     VGHFKNAILPDVTTFRESLPIISEMLAEHKDKNLVMYCTGGIRCEKASAYMKHKGYKN
                     VFQLDGGIIEYTRQVKDQGLENKFIGTNFVFDERLGERISDHIIAHCHQCGAPCDTHT
                     NCANDFCHLLFIQCEACRQKYEGCCSPLCKDYLHKPQEERLAAGKAGIFNGSKFGKGR
                     YNPKQIGKNELLKEK"
     gene            1902..2354
                     /locus_tag="PKV31_00155"
     CDS             1902..2354
                     /locus_tag="PKV31_00155"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNB91286.1"
                     /translation="MEYKLASFTNRFAALIIDGILIHVAAKFLTFPFGFGFNPWNVFN
                     GNFLDFAEYSGYVGVIALVYYVFMESSSRSATIGKQVMQIKVLDENGDNLTLSKSIIR
                     NFVKVLCGNIFMLGFFFALFTKNKQALHDILGTTLVVEDRPVAADSPS"
     gene            2819..4825
                     /locus_tag="PKV31_00160"
     CDS             2819..4825
                     /locus_tag="PKV31_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91287.1"
                     /translation="MQLTIRNFYLSSIPAGILIRLLGPVLCLFFSVFISIGQPVSTDR
                     AREVSEYTDTLGRTDTTHNDLLADLFDNDTVIVTYMRYGDLFTLLPFEDSLLSLSLRQ
                     MDVARSQNVEYFTLGNIGSPAYSPLVRPVTRGAFDMGNHGYDLYRITFDNFRFYRPAT
                     PYSHVRYGTTTGIRDDNIFTGKLSRNFAKKLTVNVDYSRFNQVGEFLRDRMRNTTFGV
                     GFEQSHWNGRYKTHLIYIYNQFLRDENGGVAEYLTDITGISGKRLSMPVNISNGGSRY
                     REWGVLLNNDLTLLRSRDSLSASSGKGLHIGYEVSYHRTAHTFYDTDVRTKEDSTYYR
                     LFNTDTRGIRNSYIHQVFLNRAYISAEGKTPTTTGVTSDYLRGGIALERNFLTYLPTD
                     SAFTLLKLEGAGHWNLAKVAGVSAKGYYLLSSLTPVFDVEGTLSGKLIKWLHARAWIR
                     LSNLPPSYIFNHLNLNSSRIFHAEAKNIFRTYFGGAVNLPGIGLELSLHQNIVTNHTF
                     FDNDWRMQQTAKAISVTGIQAQYNLKAGPFHLDNSVLWQASTDKDIRVPGLAGSHAAY
                     WEASLLKRRLLINIGAEVRYLTAFDRIGYSPLLFSFYNTDAGFQSSMLTYDAFISYKI
                     RLLRGFIRADNINSLYDNSAQYQIAPYPIDAFAIRIGFDWIFNN"
     gene            5227..6984
                     /locus_tag="PKV31_00165"
     CDS             5227..6984
                     /locus_tag="PKV31_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB91288.1"
                     /translation="MSKNIDFNLLLRVVRFSFDYKRMFYAALIMALMLAPLGALRPYL
                     INLGVDKYILNSDMSALLTLCWVIVSLVIVEAGLRFFFNYLTESLGIHVTRDLRIKIF
                     NKIVGQRVRQFDQTPTGMYITRIINDIESIKTIFSEGLLTIIADIITIIAILIMMFVT
                     SWQLTLACLVVMPLMVIATVIFNKKVKGSFQRVRAQLANMNAFLQEHLSGMTIIQAFN
                     AQKREAARFREINRNYTRANLDSVLYYAVFFPVVDIISAAVLGLMVWWGARGIMDGVV
                     TLGAMVAFPIYINTLFRPIRMLADKFNSMQQGLVAAERIFNLMDDTSVNEDSGHLQLK
                     NVEGHIQFDHVSFEYVEGNPVLHDVSFEVPRGTTLAIVGSTGSGKSTIINLLMRFYDY
                     NQGFIRLDGIELRDISKTSLRSHISLVLQDVFLFSGNVRDNITLKNPDITDEAIFKAA
                     KMLDAHRFIMALPGGYDFLVSERGYNLSSGQRQLISLVRALVYNPSILILDEATASID
                     TETEAIVQFAIDKLLRGRTAIIIAHRLSTIRNADNIMVLEHGRILEQGSHDMLLQNSG
                     SRYSELYNIQFEHKLSAGL"
     gene            6981..8075
                     /locus_tag="PKV31_00170"
     CDS             6981..8075
                     /locus_tag="PKV31_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91289.1"
                     /translation="MKNLFTLNYIGQALLAMLLIQQVDAQVTQFYKRPVSDSTLSEGF
                     SLHGSSLLYFRNNEYFSGAYPGFTALGANNELSGNYRHGRHFISTGMMYSLAFGQPMR
                     HRVLPVITYQYSLARHLNIVMGTLSGPAEHKLPQELLGLDRYLYHPLEYGLQFTYQPG
                     WMDADIWIDWRNNIMHGDSAQEEFTQGSRFIFHLLNKGGWNIDANAILLFYHNGGQID
                     RSDAPTMTRINYSVGMNLSRQISRWKYSISGQYFGFYNDEDFTAFPWKEGNAYSISLQ
                     AENATLCLGTAFKNFHHFFTLNGDQVFSIFNQYESRIYGESKKLVTGFARIQLMNIKN
                     FKIGFGAELYHDLNSRHTDYNYNIIGRFLF"
     gene            complement(8094..8543)
                     /locus_tag="PKV31_00175"
     CDS             complement(8094..8543)
                     /locus_tag="PKV31_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91290.1"
                     /translation="MKRFLRKLLLYVIGFGIGLLILANVTSYSKGERTGHIVKLEKKG
                     LLFKTWEGSLDMSIFQGARPNKGNVENTLWTFTVSDDKIAQQIQDANMRGNRVILHYK
                     EKYMKLFWIGDTKYIVIAVDEVKENPPQSPSDNPPIMGTQPGTGEKL"
     gene            complement(8619..9587)
                     /locus_tag="PKV31_00180"
     CDS             complement(8619..9587)
                     /locus_tag="PKV31_00180"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNB91291.1"
                     /translation="MKSIIGCIVFALCLASHVAKGQLFVPADTFRQGRAIAITGTGLA
                     VYSGGMYLLGKAWYKDNLTNSFKFFNDNGEWLQMDKVGHVFSAYNEAGFVHDLYRSAG
                     VRERKALWLGIGAGLLAQTSIEIFDGFSDRWGFSWGDVAGNFAGLALFGFQQAAWHEQ
                     KLKLKVSSDFRSYSTSDILPLSGSESDNLHRRAGELFSNNLAGRLLKDYNAQTYWLSF
                     SPADFSSQRFWPAWLDISIGYSGENLFGGYGNTWDYQGNTYQLSGRDFPRYRQFLLSL
                     DLDLTRIPCRSAFLRDVFRVLNIIKIPAPTLELNSLGKLKGHWIYF"
     gene            complement(9605..10126)
                     /locus_tag="PKV31_00185"
     CDS             complement(9605..10126)
                     /locus_tag="PKV31_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91292.1"
                     /translation="MRRMYYLSSIHAGIGMVDQPVNFNLEYNGTFSSNIAKKLLRTGG
                     AEYNYILNYSVNAFGPSFNFALGDKLCLGAAGLYNIISIKDYYTDTKDRTSLASSNII
                     SVKPFLGINLGSGEARVTTSLRFFANLPFKAVDITNVYSKLDPEARAGLKDDDLNPKF
                     TSVGVSLIIHNRM"
     gene            10383..11510
                     /gene="dprA"
                     /locus_tag="PKV31_00190"
     CDS             10383..11510
                     /gene="dprA"
                     /locus_tag="PKV31_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNB91293.1"
                     /translation="MSLNSTSYPEEEIYYGLALALIPGIGHIYAKTLLSYNGSFRQIF
                     HSKGSQLLKTPGMGKELIRRILNFTDFDKVEEELKYIQDHQISPLFYNHPDFPQRLLY
                     FNDSPFLIFRKGNAGLNSTRTLGIVGTRSPSQAGIRWVNDLISDLKDSGIAIISGFAY
                     GIDITAHRASIREGLETVGVLAGGLENIYPALHRKYIDDICRHGSIITESFSHVLPDK
                     QRFPMRNRIIAALSDGLIVVESGAKGGSIITAEFAFSYNKNLMAVPGKPCDLMSKGCN
                     ILIKTQKASMVEDSSDVFREMDWHLGNQSDKYAIQTEMFNSLSDKEQSLITFLIEKGE
                     VSVDEIIKNLDIRNSELASMILSLTLDGLIVQLPGSRLAIG"
     gene            complement(11511..11957)
                     /locus_tag="PKV31_00195"
     CDS             complement(11511..11957)
                     /locus_tag="PKV31_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB91294.1"
                     /translation="MNSGNMLEMMPEVQASDKVLAALHDSLEIELDTMMTRFRNNYNN
                     ALKAVNEGTMTKIKQAEVEKELQDEQARIAEYQKQAQLVVGQRRQKLLAPVIDKLNAA
                     IIEVGKEKGYSFIFDVSSGGMLYVNKSEDVSSLVASKLGLKLDDKN"
     gene            complement(12106..12315)
                     /locus_tag="PKV31_00200"
     CDS             complement(12106..12315)
                     /locus_tag="PKV31_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNB91295.1"
                     /translation="MFSSLLLLGLGVPEGILIFFALLLLFGGKKIPELMRGLGKGIRE
                     FNSARANIEEEIKTGMREEDKGKKE"
     gene            complement(12400..13353)
                     /locus_tag="PKV31_00205"
     CDS             complement(12400..13353)
                     /locus_tag="PKV31_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HNB91296.1"
                     /translation="MSSRAIPLVDLSKYTQGTEEEKEQFVEALGNAFHNVGFVGVINH
                     GIPKQLVDEFYSYSKAFFALPLESKEGYEISGLAGQRGYTAFGKEHAKQSKVGDLKEF
                     FQIGQTVTDDDPIKSQYPANVRVAEMPEFTETGDSLYKAFEKAGGHLLAAIGEYLGLG
                     ADYFKAKVHNGNSILRAIHYPPITKEPESAIRSEQHEDINLITLLVGASAGGLQVLNL
                     QGEWLDVVPEADEIVVNVGDMLQRLTNKYLISTTHRVVNPPREEWHRPRLSIPFFLHP
                     RSEMDLTCLESCVTPDNPLHFEPITAGEYLNERLREIGLKK"
     gene            complement(13480..14913)
                     /locus_tag="PKV31_00210"
     CDS             complement(13480..14913)
                     /locus_tag="PKV31_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNB91297.1"
                     /translation="MKKLLIIALIPFLLLTSCVNSLEDYNINSKSPSKVAPDPMFAYS
                     TKVLADALNSVNVNTNVFRFYAQYLTATTYLDEPRYDMTTRTIPQALWNTLYSSTLTD
                     LNEAKKLINADNAIVPEVKANKLAAIQIVEVYTWSVLVNTFGNIPYTQALDVNNSLPK
                     YDDAKTVTADILSKLDAALASIKVDAGGFGSSDLLYGGDMSKWVKFGNSLKLKLAMVI
                     ADADAATAKTKASEAASNLSNLITSNGDNALFPYLSGAPNNNPFSNDVPPLSARQDFV
                     AANTIVDLMNNLNDPRRASYFKALADGTYKGGQYGFTSQYSAHSTISTKVSATTLPGD
                     FLDAAEVNFLLAEAVERGFISGNAEEFYNNAIRASFEYWGADGADDYLAQNGVSYATA
                     GSTWQEKIGIQKWVALYNRGYDAWTDWRRLDFPELKKPDAPNVPEIPKRLIYPIIENT
                     LNATNTKDAGAAIGGDDSATRLWWDVK"
     gene            complement(14933..18148)
                     /locus_tag="PKV31_00215"
     CDS             complement(14933..18148)
                     /locus_tag="PKV31_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNB91298.1"
                     /translation="MKKTMFTLFLLVLGFVVSYGQRTISGMVTDDKSEPLVGASVLIK
                     GTQDGTVTDVDGKFSLRVPSGQATLVVSYTGYVTKEVVLDGVSNTVSVSLETDKRILD
                     EVVVTAYGLERKRNELSVSAQKVTGDEVNVVRSNDFVSALNSKVAGLDIRTGNTMGGS
                     TNVVLRGYKSITGNNQALFVIDGVPAANQTLTSSSTAQGREGYDYGSSAADINPSDIE
                     SITVLKGAAAALYGSRGANGVIIITTKKGKKDRLDVTLNSGLSWGKIDRSTFVTHQDQ
                     YGAGYGPDFYSYNGGSGFLGSDIFSPGVEEPFVPFTEDASYGAKFDPNFLVYDWRSVD
                     PLNPNYKKKSPWVAAPTDVAEFYETAFSSNQSISIEGGGSTSTFKVGYTRNDEKGVMP
                     NSSLYKNMFSIGGSLEASSKFKISSNFNFSNEAATGRQGIGYGNRNPNTMFRQWYQTN
                     VDVSDLKAAYELHEKNATWNWADITAENPIYWDNPYFARYKNYTTDSRNRYFGNIMAT
                     YTIMPGLSLVGRAGGDISFDQIEERNNKSSVDLGSYSVTNRSYKEYNYDVFANYNKEL
                     SEAISLDITAGTNIRRSYLYQISASTNTDLVVDGLYSIANSLQTPTPPSEGYVPVGVD
                     GLFATATIGFNKFLFLDGTFRRDRSTTLPKANNTYNYPSVSLGFIFSEILKQDWLNYG
                     KLRASYTQVGNDAPALSVFDVYDKPTAFGSVPFFSLPARKNNPDLKPERTKSLEFGID
                     LNMFDNRIGLEYTYYDATTFDQIIPVQITAATGFTSRFVNAGSVNNKGHEVILNLTPV
                     RTKDLSWTLTFNFAKNKNEVVELYDENTKQIVLGTFQSGISLVAIPGQPFGVIKGRGF
                     SYHENGEKIVGANGYYTRKTDQILGNINPDWFGGVGSTLRWKNISLNFLIAAKIGGDL
                     YSLDQAYGQYSGLYPETAGLNDLGNPVRNTLEEGGGIILPGVKADGTPNDVRVDASNS
                     DITPFGIANNPNEAFIYDASFVKLRELNLTYSFDKSLFTNSKFVKGADISLTGRNLWI
                     LYKNLPYADPEDVYSAGNLSGHQGGAYPTVRTFGFNVKLKF"
     gene            complement(18489..19226)
                     /locus_tag="PKV31_00220"
     CDS             complement(18489..19226)
                     /locus_tag="PKV31_00220"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNB91299.1"
                     /translation="MHTKLSVNVNKIALVRNSRGADYPNLLEMSLDLEDYGAEGITVH
                     PRPDQRHIRYDDIPILRAHLRTELNIEGFPSEEFIRLVIDNKADQVTLVPDSPEALTS
                     DHGWDFEKHEDLLRAIVPVFKDAGIRVSLFVDPQPDNADKAQAVGADRIELYTGPYAH
                     RFERDGNGGVQAHTDTSRRALNIGLGVNAGHDLNLKNLAFYKRHMPHLQEVSIGHALI
                     VDAMYFGYSNAIQMYKDRLIHPEHYLG"
     gene            complement(19327..20178)
                     /locus_tag="PKV31_00225"
     CDS             complement(19327..20178)
                     /locus_tag="PKV31_00225"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HNB91300.1"
                     /translation="MQISKLDKVAGLDRASFIEHFLKPMKPVVFTDLTKDWSALQNWT
                     VENFKSKYGNLKVSVYTNKFEGTDKGFREPDKHMLFGDFLDLLVSSEPCDLRLFLFNI
                     FKYAPELRGDFDLPDITDGFYNDLPSMFFGGKGSKVAMHYGLDLSHVFLNQIYGKERV
                     VLFSPDQSRLIYQLPYSVAGNIDINNPDYDRYPALRNAKGEEVVLNPGESLFIPSGYW
                     HYIEYVEGGYSVSLRANESIRRRMKGAANIATHFIVDKGMNRLLGSKWREIKDRMVIW
                     RAQAMID"
     gene            complement(20242..20547)
                     /locus_tag="PKV31_00230"
     CDS             complement(20242..20547)
                     /locus_tag="PKV31_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB91301.1"
                     /translation="MKEITVEELKAKLDAKEDFVFIDVREPHEYDVFNLGARLIPLGE
                     FLKDVHILDDNKGDEIVIHCRSGMRSSMAVEALVNSGYTNVRNLVGGVLRWQEVFPG"
     gene            20869..21783
                     /locus_tag="PKV31_00235"
     CDS             20869..21783
                     /locus_tag="PKV31_00235"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNB91302.1"
                     /translation="MDYRTVLNEIYSVGKVMEGLGNVATYIPELSNVDPDKFGIYLLT
                     LDRQGYGIGDDKERFSIQSISKIFTVSMAIRILGESVWKRVGVEPSGSSFNSMVQLEY
                     EKGYPRNPLINPGALVIADMLISNLANPKEDLLDFVRMVSGYESITFDEKVAESEKRT
                     GFRNAAMAYMLKAFNNLDNDIDTVLDFYFHQCSITMSCKELASAFFYFANKGYTYDNS
                     IQILTESKVKRLNALMQTCGFYDESGEFAYKVGLPGKSGVGGGIIAIYPGRYTVATWS
                     PRLNSKGNSVRGMKALEMLTTFTTESIF"
     gene            21920..22732
                     /locus_tag="PKV31_00240"
     CDS             21920..22732
                     /locus_tag="PKV31_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB91303.1"
                     /translation="MKTILVPTDFSETAEKALNAAVHIGGFQQTDLVLMHTSLPTEGV
                     NSNVYAAIYIHEYLETKRKNLNLLADKVRETGYKGKVKTDNKVDFTVPSIVNAAEEFD
                     ADLVVMGSTGSSGLAGVFLGSIAGGVLSKIERPLFLITKDFTLPEGGDLLIGTDYKTR
                     PDKNSLEILRFFKEQLNFSLNLVHISHGEEVSINKEAFVNNHFDGMIDSHVDVPGAKV
                     EGALMKIKEKYQNAVLCTITRKRSWFESLFSHSVSRSLTHYSDIPLLCLQSE"
     gene            23078..23584
                     /locus_tag="PKV31_00245"
     CDS             23078..23584
                     /locus_tag="PKV31_00245"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HNB91304.1"
                     /translation="MKKLILFFLLASTTLYGQDASGEYRHEIKLNGLNLVLGNPELDY
                     EYLINSNSGAGLYANANIDRNHIWPFVYMAGAYYRLYLGGKHANGFFFEGGFNAIGFD
                     DTEFGKNKVSNGWGPSFGIGGKFLIKKTIVMEFFGGLGRNFKATDEYNNYDIPVFPRW
                     GISIGKRF"
     gene            23603..24319
                     /locus_tag="PKV31_00250"
     CDS             23603..24319
                     /locus_tag="PKV31_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNB91305.1"
                     /translation="MNYNIEISGVRVRVEVRYGPTRNIRLFYRNNTARLTVPKGLREK
                     DLIRAKGKLHEWLINLATAKPHLFVSDEYRALTKEKIILLGQCYHLKFKATQSNRITI
                     HRKEDTLAMEGTDEFFRDPEQMSDFIPRLFNKVFARDIEQRVRAINDQTLRANIGRIS
                     LKSVVSRWGSCSPDNNIMISNRLLLAPTEILDHVIIHELAHTVHRDHSRQFWRVVAQY
                     DPAMKEKNDWLKQNGHTLNY"
     gene            24664..24825
                     /locus_tag="PKV31_00255"
     CDS             24664..24825
                     /locus_tag="PKV31_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91306.1"
                     /translation="MKQIFIGLSLTILSFIRCSREEDSIKNPDALYFDSLDIPALMFI
                     AKEYDPGNN"
     gene            24898..25896
                     /locus_tag="PKV31_00260"
     CDS             24898..25896
                     /locus_tag="PKV31_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNB91307.1"
                     /translation="MKKYIFLIPMIAFFLTSCSKEVDKISSTPKLTLTLSPPPFSVPA
                     GTKFSADVSFNQYPNSTFDIFMPSSGKPTALAIFIHGGGFVGGDKSSIYSQGQDDIKS
                     YLDHSIAFATINYRFKNENPDSNILISLADIKRCIQFIRYHASSFNIDKEKIACYGGS
                     AGGGASIYLAFHPDMADPGNIDPIMKESTRIIAAGHLTSQCTYDPIRMEAMYAESGID
                     LFAIPGIEQSVLADFRADSISQFYTDPRLVALRHELEMLGWMTPDDPEFFVSNENPAA
                     PPSDRRTALHHPLQGKALDNKATEIGLAHVTHIPSLNLLAPNHETISQFMIRKLSE"
     gene            25972..27231
                     /locus_tag="PKV31_00265"
     CDS             25972..27231
                     /locus_tag="PKV31_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91308.1"
                     /translation="MKTYVLLLLMFPIFNLSAQSYCTSGQYSEVGYFNPDEITIRTNV
                     EYGRAETFQGITIPLRMDIYMASTDVDTTIHRPLIVLIHGGGFLNGKKNNYATTASLL
                     AQAGYVVSSIDYRLGWDNGTSPCTGSLPSLLAAEYRAMQDAHAAVRYLIENQHLYHID
                     TGAIFLGGSSAGSTTALQLAYYSPAEASTLSSTLGGLNESGNAFRHTFRVRGVLNECG
                     GIGDTSFIKSDTPIPMIAFHGSADRIVPIDTGRILDCYTPSRYHFIAGSRAIYHTLRN
                     LGVCTELNVYPGGPHCAYSNDTLIAKRTACFFKGILCHDCASLDQEGEVPASCAMVAK
                     DYLIQQDRGVKLYPNPASERLTILLIGETETVAKYSILSMSGRNQMQGILPDKQSTID
                     VSGLAQGMYCLTIDMPGKTITTKLIKL"
     gene            27956..>28478
                     /locus_tag="PKV31_00270"
     CDS             27956..>28478
                     /locus_tag="PKV31_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB91309.1"
                     /translation="MANAPKQNEKKTNSGSILPYIAIPLAFVIGIILYKFVFGAASHF
                     KNGDPNNDPLPGDYMGVIYKGGFIVPILLGLLFITIIFSIERFITIMRAAGTGPVDKF
                     VRNVQYSLSKGDINGAIAACDKQKGSVGNVVRSGLTTYNKMTSESGMTKEQRIAAIQK
                     EVEEATSLELPSLE"
BASE COUNT         8362 a         6341 c         5682 g         8093 t
ORIGIN      
        1 ggttatgaag aagctctgtt taactatctc accccggaac atctcgatca gttttcatcc
       61 tccgagctca acagctatgc ctggtatgta tatgaaagta ccgaaggagc tgacaaggcc
      121 aaggcagcta ttccatgggc ggaagcctcc gtcgccaaaa aaagcgaata tgccaataac
      181 gacacccttg cctggttata tttcaaggcc ggtgatgtag aaagggcaaa ggaaatagcc
      241 aggaaggcaa tcgaactggg caaggctgcc ggtgaggata cctcttctac cgaggaattg
      301 ttgcagaaat aaaagtaatg atgaatgctg ccgggatgtt ttaggaagtt cctgaaattg
      361 aaaggcgact acaacccttc aacatgtgcc tcattgagtc atttgtcttt agaaggattg
      421 tgcatggatg atactgtcac ccggattgtt ccggccagca ttatatctcc ggtgtaaata
      481 ttaaattcat ggggattcat gagttaggtg aattcaattc ccgaaaaagt aattcgcccg
      541 gtattgcaat tagacattgg gaaattcagt actacatttg agcctgagag atacaaatcc
      601 ataagtatgt aatgacgcat aggccagatc agagatatag gccaaaatct caggaataac
      661 catccggtag aatcactatt tccacacatt ttgtattttt gccctgaaaa tcaacccaat
      721 cgctgcttaa gcgttaatat ttaatcaaac gaacagagaa atgcatttac ataacagggt
      781 taatggccgt gaactaaagg ccaggatgag acaatccgat gaaaaaagga ttactatatc
      841 attttataaa taccacaaaa ttgaaaaccc acaatttttg cgggaccatc tgtatctgga
      901 atgggccaaa ctgggcgtct tagggagaat ctatttggct aatgaaggca tcaatggaca
      961 gatatctgtt ccggaagaaa gtcatgaggc attcaaagcg gctttcgaac aacttgactt
     1021 tatgcgtggt atcagattga attatgccgt agatgatgac ggcaaatcct ttttcaagtt
     1081 gaagatactt gtaagaaaga aaattgttgc agatggactt aatgattcaa ccttcgatgt
     1141 aacaaactcg ggcatccacc tcagcgcaaa agaaatgaac gaactcctcg agcaatcaaa
     1201 tacagtggtg gtcgatatgc gcaatcatta tgaaagcgaa gtcggacact tcaaaaatgc
     1261 catcctgccc gatgtgacca ccttccggga atcactgcct atcataagtg aaatgctggc
     1321 tgagcacaaa gacaaaaacc ttgtcatgta ttgtaccggt ggaattcgct gtgaaaaagc
     1381 aagcgcttat atgaaacaca agggatataa aaatgtattc cagcttgatg gcggtatcat
     1441 tgaatataca cgacaagtca aagaccaggg actcgaaaat aaattcatcg gaaccaattt
     1501 tgtttttgac gaacggcttg gagaaagaat cagcgaccac atcatcgccc actgccatca
     1561 atgtggcgca ccatgtgata cccataccaa ctgtgccaat gacttctgcc acctgctttt
     1621 tatccaatgt gaagcatgcc ggcaaaaata tgaaggctgt tgctcccctc tctgcaaaga
     1681 ttatctgcat aaaccacagg aagaaaggct ggctgccgga aaagcaggaa tcttcaatgg
     1741 gtcaaaattc ggaaaaggcc gttacaaccc caaacaaatc ggaaaaaatg aactgttaaa
     1801 agaaaaataa acgtttttgg tcgatacttc tgtttattgg aaactattat gtattgcaaa
     1861 gtagttaaaa gagtatattt ttaccttaaa aaaacgaaaa aatggaatac aaattggcat
     1921 cttttacaaa tcgatttgct gctttgatta ttgacggaat actgatccac gtagccgcaa
     1981 aatttctgac attcccattt gggtttggat tcaatccctg gaatgtattc aatggcaact
     2041 tcctggattt tgcagaatat tcaggctatg tgggagttat tgccttagta tattatgtgt
     2101 ttatggaatc ttcctctcgc agtgccacca ttggcaaaca ggtgatgcaa atcaaagtcc
     2161 ttgatgagaa cggagacaac ctgaccttgt caaaatcaat tatcagaaac ttcgtcaagg
     2221 tattatgtgg caatattttt atgctgggat ttttctttgc gttattcacc aaaaacaaac
     2281 aggcgctgca tgatattctt ggcacaacat tggtggtgga agacagacca gttgcggctg
     2341 attctccatc ctaaggcgac acgtatctaa gaagaacaga gatgggctca ttggcacttc
     2401 accaccaatg ggctcattcc tgtaacccat ctctattgcg ctccgatgat ttcaaacaaa
     2461 gggtcaaggc tattctgacc tcattctgga gactgaaatt atctataata tgggtgtgtt
     2521 gtattgaaat aataaccgga attataaaac atcttgagtg aagggaatgg ttacaaaatc
     2581 atgtaactgt agttcctacc aatccttccg ctaactaatt tttgtgtttg atccggtttg
     2641 ttatcctgtc cacaatcttt ttatcatccg ggtaagcgac atctaataca ggcgaagagg
     2701 acatgattcc aacagaaact atatccgtta aaaccaaatg atcactaatc ttggtttatt
     2761 ttttataaat tataattgca aagccataag tttgcacctt gtaatgatgc cggaccctat
     2821 gcagcttacc attcgcaatt tttatttaag ctcgatcccg gccgggatct tgatccggtt
     2881 gttgggcccg gtgctctgtc tctttttcag tgttttcata tcaatcggcc aacctgtctc
     2941 caccgacaga gcaagagagg tttctgaata tacagatact ctcgggagaa cggataccac
     3001 acacaatgac ttattggccg acctcttcga caacgacact gtcatcgtta cttatatgag
     3061 atatggcgac ttattcactc tgctaccctt cgaagacagt ttgctctcac tatctttacg
     3121 acagatggat gtggcaagaa gccagaatgt ggaatatttt accctgggca atatcggctc
     3181 gccggcatac agccccttag tccgaccggt gacaagggga gccttcgata tggggaatca
     3241 cggctatgat ctctacagaa tcactttcga caacttccgt ttctacaggc ctgcgacgcc
     3301 gtactcccat gtgcgatatg ggacaacaac cggaatccgt gacgataata tctttaccgg
     3361 caaactgagc cgcaattttg ccaagaaact taccgtcaat gtagactatt cccggttcaa
     3421 ccaggtagga gaatttcttc gtgaccgcat gcgaaacact accttcggag taggatttga
     3481 gcaaagccat tggaacggcc ggtacaagac acatctgata tacatttaca accaatttct
     3541 aagagatgaa aacggtggtg tggcggagta tctcaccgat atcacaggca tatccggcaa
     3601 gagactcagc atgcctgtca atatcagcaa tggcggttca cgataccggg aatggggcgt
     3661 cctgctcaac aatgacctta ccttgcttag aagcagggat agcctgtctg catcttccgg
     3721 caaagggctg cacatcgggt acgaagtgag ttaccatcgc accgcccata ctttctacga
     3781 tacagacgtc cggacaaaag aggattcaac ctactaccgg ctattcaata ccgacacacg
     3841 aggcatcaga aacagttata tccatcaggt cttcctcaac cgagcctata tttctgcgga
     3901 aggaaagaca ccgactacca caggcgtaac atcggattat ctgcgcggcg gaatagccct
     3961 tgagcgtaat tttctaacct atcttcctac agactcggct ttcacgttac tcaagctgga
     4021 aggagccgga cactggaacc tggccaaggt agccggagtc agcgccaaag gttactactt
     4081 gcttagttcg ctgactcctg ttttcgatgt cgagggtacg ctttcaggca agttgatcaa
     4141 atggttgcat gcaagggcat ggatcagact cagcaatctg ccgccatctt atattttcaa
     4201 tcatctgaat ctcaattcct caagaatatt ccatgccgag gcaaaaaaca ttttccgaac
     4261 ctatttcggt ggagctgtta acctgccggg cataggcctg gaattatcct tgcatcagaa
     4321 catcgttacc aaccatacct tttttgacaa tgattggaga atgcagcaaa cagcgaaggc
     4381 tatctccgta accggaatac aggctcaata taatctgaag gcaggcccat tccatctgga
     4441 caacagtgtc ttgtggcagg caagtaccga caaggacatc cgagttccgg gcctggcagg
     4501 aagtcatgcc gcctattggg aggcatcact attgaaaaga aggctattaa ttaatattgg
     4561 tgctgaagtc agatatctca cagcttttga tcgaataggc tacagccctt tgcttttcag
     4621 cttctacaat actgatgccg gattccaatc ctccatgctt acctacgacg cctttatatc
     4681 ttacaaaata aggttgttgc gtggttttat cagagctgac aacatcaaca gcctgtatga
     4741 caacagcgcc caataccaga ttgcccccta tccaatcgat gcatttgcta tccgaatcgg
     4801 atttgactgg atatttaaca attaataccg acttgcttct ataacagtat taaaccgaat
     4861 gccggaaaaa ggatgcggct gctattcctt ctaaacaaac tttgataaaa gttctttgta
     4921 cgaataacaa attgcactca ttaaatttcc ggatttagct ataaataaaa tctccgcagg
     4981 ttcagcctta tgaaatgtgt catttgaagg gttgaacaga agctactaca aataaatggt
     5041 atctgcttgt tgtcactttt tgcaatgatc ttactattaa atagcagcat aacttttctt
     5101 cataatgaaa ttagtatgaa agatgctaaa cacaccataa ttcgttctgc agtgtccaat
     5161 tattaattta ttcctaattt tacaatgaaa tcatattgtc agtcgatatg attcccgttg
     5221 tgctatatgt ccaaaaacat tgactttaat ttacttttaa gggtagtaag attttccttc
     5281 gactacaaaa ggatgttcta tgctgccttg ataatggctc tcatgctggc tccattaggg
     5341 gcattgcgac cctatttgat caatctcggg gtggacaaat acatactaaa tagcgatatg
     5401 tccgcattat tgacactctg ttgggtcatt gtaagcctgg tcattgtgga agcaggcctt
     5461 cgttttttct ttaattatct cactgaatct ctcggaattc atgttacccg agacctgcgt
     5521 atcaaaatat tcaataaaat tgtaggtcag cgagtcagac agtttgacca gactccaacc
     5581 ggtatgtaca tcacccgcat catcaatgat attgaaagta tcaagacgat tttttctgaa
     5641 ggtttgctca ccatcattgc tgatatcatt accatcattg ctatcctgat tatgatgttt
     5701 gtgaccagct ggcaactgac tcttgcctgt ctggtagtga tgccgctgat ggtcattgcc
     5761 acggttatct tcaacaaaaa ggtcaaaggt tcgtttcagc gtgtccgtgc ccaattggcc
     5821 aatatgaacg cctttcttca ggaacatctg tccggaatga ccatcattca ggctttcaac
     5881 gcacagaaaa gagaagctgc caggttcagg gaaatcaacc gaaattatac ccgtgccaac
     5941 cttgattctg tactctacta tgcagtattt tttcctgttg tagatatcat ttctgctgcc
     6001 gtgctgggcc tcatggtctg gtggggagca cggggaatca tggatggagt ggtgaccctg
     6061 ggagccatgg tggccttccc catttatatc aatacattgt tcaggccgat caggatgctg
     6121 gccgacaagt tcaacagcat gcagcaaggc ttggtggctg cagaacgcat ctttaacctg
     6181 atggatgata catctgtcaa tgaagactcc ggtcacttgc aactgaaaaa tgtcgaaggg
     6241 cacattcaat tcgaccatgt aagcttcgaa tatgtcgaag gcaatcctgt cctgcacgat
     6301 gtttcctttg aggttccaag aggtactacc ttagccatcg ttggaagtac cggaagcggc
     6361 aaatcaacca ttatcaacct gctgatgcgt ttttacgatt acaatcaggg attcatccgt
     6421 ctggacggta tcgaactgcg cgatataagt aagaccagcc tccgttcgca catcagcctt
     6481 gtattgcagg atgtctttct tttctcaggc aatgtccggg acaatataac cctgaaaaat
     6541 ccggatatca cagatgaagc aatcttcaag gctgctaaaa tgctcgatgc ccacagattc
     6601 ataatggcct tgcccggtgg ttatgatttc ctggtttccg aacgcggata taatctcagc
     6661 tccggccaaa gacaacttat atctctggtt cgcgccctgg tgtacaaccc ttccatccta
     6721 atcctggatg aggccacagc cagcatcgat accgaaacgg aagccatcgt ccaatttgcc
     6781 attgataagt tgctgcgggg taggacagcc attatcatcg cccatcgctt gtccaccata
     6841 cgtaacgctg ataacatcat ggtactggaa cacggtcgta tactggaaca gggaagccat
     6901 gacatgttgc tgcaaaactc cggcagcagg tacagtgagt tatacaatat tcaatttgag
     6961 cataaattaa gtgccgggct atgaaaaatc tttttacttt gaattatata ggccaggctc
     7021 ttttggcgat gcttctgatt caacaagttg atgcgcaggt gacccaattc tacaagcgtc
     7081 cggtctctga ttctactctt tccgaaggct tttctttgca tggatcttct ttgttgtact
     7141 tcaggaataa cgaatatttc tccggtgcct atccagggtt tacagcattg ggagctaaca
     7201 atgagctgtc aggcaattat cgacacggca gacacttcat cagtacaggc atgatgtaca
     7261 gtctggcttt cggtcagccc atgcgccacc gggtacttcc tgtcattacc taccaatata
     7321 gtctggccag gcatctgaat atcgtcatgg gcaccttatc cggccctgca gagcataaat
     7381 taccacaaga actccttggt ttggaccgat atctttatca tcccctcgaa tacggactac
     7441 agtttaccta ccaaccgggg tggatggatg ccgatatatg gatcgattgg cgaaataaca
     7501 tcatgcatgg agattctgct caggaagaat tcacacaggg aagccgtttc atctttcatc
     7561 tgctgaacaa agggggatgg aacatcgatg ccaacgccat tcttcttttt tatcacaatg
     7621 ggggtcagat cgatcgtagc gatgcgccga ctatgacccg aatcaactat agtgttggga
     7681 tgaacctttc caggcagatc tcccggtgga aatactcaat atctggtcag tatttcggat
     7741 tctacaatga tgaagacttt accgcctttc cctggaaaga aggcaatgcg tacagtattt
     7801 cactgcaggc agaaaatgct acattatgcc ttggtactgc ctttaaaaat ttccaccatt
     7861 tcttcaccct caatggcgat caggtattca gcatcttcaa tcagtacgaa tcccggattt
     7921 atggagaatc caaaaagttg gtcaccggct ttgcccgtat tcagttgatg aatattaaga
     7981 atttcaagat tggttttggc gctgaattat accacgacct taattccagg cacacggatt
     8041 ataattacaa tattatagga cgattcctat tctgagaatg gatatccatt agactacaac
     8101 ttctctccgg ttccgggttg cgtccccata ataggaggat tgtcagaagg cgattgagga
     8161 gggttctcct taacctcatc caccgcaata acaatatatt tggtatcacc tatccagaag
     8221 agcttcatgt acttttcctt ataatgcagg atgaccctgt tacctctcat gttggcatcc
     8281 tggatttgtt gtgcaatctt gtcatccgat acggtaaaag tccagagtgt attctctaca
     8341 ttgcccttat tgggtctggc tccctgaaaa atgctcatat cgaggcttcc ctcccaggtc
     8401 ttaaacaaca agcccttctt ctcaagtttg acgatatgtc ctgtacgctc acctttacta
     8461 tagcttgtca cattagccaa aatcaataat cctattccaa aaccgataac atataacagg
     8521 agtttcctaa ggaatctttt cattcgtttt gatatttaat ccaattaaca aagatagcaa
     8581 tgaaatgatt cattcagatt gatatattca tttctatttc aaaaatatat ccagtgaccc
     8641 tttaattttc ccaatgaatt taattccagt gtaggtgcag gtattttgat aatattgagc
     8701 acacgaaaga catccctgag aaatgctgaa cgacaaggga tgcgcgtcag gtccagatcc
     8761 aatgaaagaa gaaattgacg ataccgagga aaatcccggc cacttagctg gtaagtattg
     8821 ccctgataat cccaggtatt gccatagccc ccaaataaat tttctccact ataacctatg
     8881 gatatatcta accaggccgg ccaaaatctt tgtgatgaaa aatctgccgg gctgaagctg
     8941 agccaatagg tctgagcatt ataatccttt aacaaccgac ccgccaggtt gttgctaaac
     9001 agttctcctg cccttctgtg tagattgtca ctctcagaac cggacaatgg caatatgtcg
     9061 gatgtagaat aactcctgaa atcagaagac actttaagct tcaatttctg ctcgtgccag
     9121 gctgcctgtt gaaaaccaaa caatgccaaa ccggcaaaat tcccggctac atcaccccat
     9181 gaaaaccccc atcgatcgga aaaaccgtca aaaatttcaa tgcttgtttg tgcgagtaag
     9241 cctgctccta tgcctaacca caaagccttt ctttctctta cgccagccga tcggtacaga
     9301 tcgtgcacaa acccggcctc attgtatgca ctgaaaacat gccccacctt atccatctga
     9361 agccattcgc cattatcgtt gaagaatttg aaggaattag tcaggttatc cttgtaccat
     9421 gccttaccca acaagtacat tcctccacta tagacagcca atcctgttcc tgtgatggca
     9481 attgccctgc cttgcctgaa ggtatccgcc ggaacgaaca attgaccctt ggcgacatgg
     9541 gaggccaggc acaaggcaaa tacaatacat ccgattattg atttcatttg ccggcagcca
     9601 ttcatcacat tctgttgtgg ataatcagac ttactcctac gctggtaaac ttcggattaa
     9661 ggtcatcatc cttcaacccg gcccttgctt ccggatccag ttttgaatat acgtttgtaa
     9721 tatcaacggc cttaaagggc agattggcaa agaagcgcaa ggatgttgtt acccttgctt
     9781 ctccggagcc aagattgatt cctaaaaatg gctttacgga tataatattg gaactcgcca
     9841 gagaggttct atccttggta tccgtataat aatccttaat ggaaataatg ttgtacaatc
     9901 cggctgcgcc aaggcagagt ttatctccga gcgcaaaatt aaagcttggg ccaaaagcat
     9961 tgaccgagta attcaggata taattatatt cggcaccacc tgtacgcaac agttttttag
    10021 caatatttga agaaaaggtg ccgttgtatt caagattaaa atttaccggt tgatcaacca
    10081 tcccaattcc ggcatgaatg ctgctcaggt aatacatccg cctcatggtc ctcgccttct
    10141 caggaaggta ggaattgaat gaatgaatga tagggcgtaa cggattgttg ataatggtcg
    10201 atccggaata tccgacatca atggcaaact gaccgaatca ggccgttgtg atggccataa
    10261 taaagacaaa cggcagtaaa gcctttccaa tcatatctat cttttttaaa aaaatgccgg
    10321 ttaaagacta taatcagcaa aaataagatt aaatttgggt tttaatcacc aaatgatatt
    10381 tcatgtcact taattcaaca tcctatccgg aagaagaaat atattatgga cttgccttgg
    10441 cattgatacc cggcatcgga catatatacg ccaaaacact attgagttat aatggtagct
    10501 ttaggcagat ctttcattcc aaaggttccc aattgctaaa gactcccggc atgggcaagg
    10561 agctgatccg ccgaatcctg aacttcacag attttgacaa agtggaggag gagttgaaat
    10621 atatccaaga ccatcagatt tcgcccttgt tttataacca tccggatttt ccacaaaggc
    10681 tattgtactt caatgacagc cctttcttaa ttttcagaaa agggaatgct gggctcaact
    10741 ccaccagaac tctaggtatc gtgggcacca ggagtccaag tcaagcgggt atccgatggg
    10801 tcaatgacct gataagcgat ctgaaggatt ctgggattgc aattatcagc gggtttgctt
    10861 atggtatcga tattacggcc catcgggcaa gtataaggga aggcctcgag accgtaggcg
    10921 tgcttgccgg tggacttgag aatatttacc cggcccttca ccgaaaatat atcgatgata
    10981 tttgccgaca tggatccatc attaccgagt ctttttctca tgttctaccg gacaaacaac
    11041 ggtttccaat gcgcaacaga atcatagctg cattatcaga cggattgatt gtggttgaat
    11101 ccggtgcaaa gggaggcagt atcattactg cagaatttgc cttcagttac aataaaaatc
    11161 taatggctgt ccccggcaaa ccctgcgacc tgatgtcaaa aggatgcaac atcctgatta
    11221 aaacccagaa agcatcaatg gttgaagaca gttcagatgt ctttcgggaa atggattggc
    11281 atttgggtaa tcaaagcgac aagtacgcaa tacagacaga aatgttcaat agcctttctg
    11341 acaaggaaca atccttaatc acttttctca ttgagaaagg cgaagtgtcc gtggacgaaa
    11401 taatcaaaaa ccttgatatt agaaacagtg aacttgcttc tatgatctta tcgcttaccc
    11461 ttgatggcct gattgttcag ttgccgggaa gcagattagc tataggctga ttaattttta
    11521 tcgtccagtt taaggccaag tttggaagca accagggagc tgacatcttc cgatttattg
    11581 acataaagca tacctcccga gctaacatcg aagataaagg aataaccttt ctccttaccc
    11641 acttcaataa tcgctgcatt gagcttatca atcactggag ctaaaagctt ttgtcttcgt
    11701 tggccgacta ctaattgtgc ttgtttttga tactcagcaa ttcgggcctg ctcatcttgc
    11761 aattccttct ccacttcggc ttgcttgatc ttggtcatgg tgccttcatt gactgctttc
    11821 aatgcattgt tataattatt cctaaatctg gtcatcatcg tatccaactc tatttcaagc
    11881 gagtcatgca atgcagccaa aaccttatcg gaagcctgaa cctccggcat catttccaac
    11941 atattgccgg aattcatatg tccgaacttc tgctgactaa tagatacaaa tggaatacac
    12001 aaaatcagtg taaatagtaa aatcgaattt ttaaacataa tatagcttat caatattgcc
    12061 gggaaacggc aaagtcaaaa attaaagaaa aagcaataac ttcaattatt ccttttttcc
    12121 cttgtcttcc tcacgcattc cggttttgat ttcctcttca atatttgctc gggctgaatt
    12181 aaactcacga atgccttttc ccaacccccg catcagctca ggaattttct tcccaccaaa
    12241 caataaaaga agtgcaaaga aaatcaaaat accttccggc actcccaatc ccaacaacaa
    12301 tagcgatgaa aacatgatca agatttatac tttatggtta taatttaccc tgtaaaatga
    12361 taaacgttta ttggggtaaa aatggttaat tacttttctt tattttttca aaccaatctc
    12421 ccttaacctt tcgttaaggt attcaccggc ggtaataggc tcaaaatgca atggattgtc
    12481 cggtgtaaca caactttcca aacaagtaag atccatttcg ctcctgggat gaaggaaaaa
    12541 cggaatcgag agtctcggcc ggtgccattc ttcacgtgga ggattaacca cccgatgggt
    12601 tgtagatata agatatttat tcgtcaatcg ctgaagcata tcgcccacgt tgacaacaat
    12661 ctcatccgct tcaggtacaa catcaagcca ctcaccttgc agattcaaca cctgaagtcc
    12721 tccggccgat gctcctacca acaaggtaat caaattaata tcttcatgtt gttcggaacg
    12781 gatagcactt tcgggctctt tagtaattgg tggatagtga atagcacgga ggatactatt
    12841 accgttgtgc acctttgctt tgaaataatc agcgccaagt cctaaatatt caccaatagc
    12901 agccagcagg tgtcctcctg ccttttcaaa tgccttatac aagctatcgc ctgtttctgt
    12961 aaactcaggc atctcagcta cacgcacatt ggcaggatat tgagatttta ttggatcatc
    13021 atctgtaact gtctggccta tctggaaaaa ctctttaagg tcacctacct tggactgttt
    13081 tgcatgttct ttcccaaaag ctgtataacc tctttggcct gcaagtcctg atatttcata
    13141 accttccttt gattccagcg gcaaggcaaa aaatgctttg gagtaagaat aaaattcatc
    13201 taccaattgt ttggggatgc catggttgat taccccaaca aagccaacat tgtgaaaagc
    13261 atttcccaat gcttcgacaa attgttcttt ttcctcttca gtcccttgtg tgtatttact
    13321 taaatcaacc aaaggaatag cacgtgaact catttttttc cgatttgagg gacaaagata
    13381 ataaaaatca agttcttaga gtctattctt aactccaatt ggaccaaaaa aaaggtggtc
    13441 gaaattcgac cacctcatta ttatccgtta atacttaact tatttaacgt cccaccacag
    13501 tcttgtagca gagtcatctc cacctattgc tgcaccagca tccttagtgt tggttgcatt
    13561 caatgtattt tcaataattg gatagatcaa acgcttaggt atttcaggaa cgttcggagc
    13621 atccggcttc ttgagttcag ggaagtccag tcttctccag tcagtccagg catcatagcc
    13681 cctattgtat agagctaccc acttttggat tccaattttc tcctgccagg tgcttcctgc
    13741 tgtagcataa cttacaccat tctgagcaag ataatcatca gccccatcag ctccccaata
    13801 ttcgaaagat gctctaatcg cgttattata gaactcttct gcattaccgg aaataaatcc
    13861 tctttcaaca gcttctgcaa gcaagaaatt tacttcggct gcatccagaa aatcacccgg
    13921 caaagttgtt gcagacacct tcgtactaat ggttgaatgc gccgaatact ggctggtaaa
    13981 gccgtactga cctcctttgt aagttccatc agcaagtgcc ttaaagtatg aagcacgtcg
    14041 agggtcgttc aagttattca tcaaatccac aatagtattc gctgcaacaa agtcctgacg
    14101 tgcactcaaa ggtggcacat cgttagaaaa tggattgttg ttaggtgcac cactaagata
    14161 agggaacaat gcattgtctc cgttggatgt aatcagatta ctcagattgg acgcagcttc
    14221 gcttgccttg gtcttggcag ttgcggcatc agcatcagcg attaccatag ccaatttcaa
    14281 tttcaatgaa ttgccaaatt taacccactt ggacatatcc ccaccgtata acaagtctga
    14341 acttccaaat cctccggcat caaccttgat tgaagccaaa gcagcatcca gcttactcag
    14401 gatatcagct gtaaccgtct tcgcatcatc gtatttaggc aaagaattgt tcacgtctaa
    14461 agcctgagtg tacgggatat taccaaaagt gttgaccaaa actgaccatg tataaacttc
    14521 cacgatttga attgcagcca acttattggc cttaacttcc ggaacgatgg cgttatccgc
    14581 attgatcagt ttcttcgctt cattcaagtc agtcaaagta gaactataca gtgtattcca
    14641 aagagcctga ggaatagtcc ttgtagtcat gtcatatctt ggttcatcca gataagtagt
    14701 agctgtaaga tactgtgcat agaatctgaa tacgttggta ttcacattca cactgttcaa
    14761 agcatctgca agaacttttg tagaatatgc aaacatcgga tccggtgcga ccttagaagg
    14821 cgacttcgaa ttgatgttat aatcttccag actgttgaca cagctggtca acaaaaggaa
    14881 aggaattaaa gctattatta ataatttttt catcattttt taatttaatg gattagaact
    14941 tcaatttaac attaaatcca aaggttcgaa cagtaggata agcacctcct tggtgtccgg
    15001 aaaggttacc cgcgctatat acatcttcag gatcggcata tggtaggttc ttataaagaa
    15061 tccaaaggtt acgtcctgtc aatgaaatat cggcaccttt tacaaacttg gagttggtaa
    15121 acaagctctt gtcaaaagaa taagttagat tcaattctct caatttaaca aatgatgcat
    15181 catagataaa tgcttcgttc gggttgttag caataccgaa tggagtaata tcagagttgc
    15241 tggcatccac tctcacgtca tttggtgttc catcagcctt aacacctgga aggataattc
    15301 caccaccttc ttcaagtgtg tttctaactg ggttacccaa atcgtttaag cctgctgtct
    15361 ctggatacaa tcctgagtac tggccataag cctggtcaag agagtacagg tctccaccaa
    15421 tcttagctgc aatcaggaaa ttcagactga tattcttcca tctcagtgtg gaacctacgc
    15481 cgccaaacca gtcagggttg atgttaccaa gaatctggtc agtttttcta gtataatatc
    15541 catttgcacc gacgatcttc tcaccatttt catggtatga gaatcctcta cccttgatta
    15601 caccaaatgg ctgtcccggg atagcaacta agctgatacc gctctggaat gtaccgagta
    15661 caatctgctt ggtattttca tcatatagtt caacaacttc gttcttattc tttgcaaagt
    15721 tgaaggtcaa tgtccaactt aaatccttgg ttctcaccgg tgtcaggttt agaatcactt
    15781 catgtccctt gttgtttact gatcctgcat ttacaaacct tgaagtaaat cctgtggcag
    15841 cagtaatctg aacagggata atctggtcaa atgttgttgc atcatagtat gtatattcca
    15901 atccgattct gttgtcaaac atgttcaggt ctataccgaa ttccaatgat tttgttctct
    15961 caggcttcag atcaggattg ttcttacgag ccggcaagga gaagaatggt acagatccga
    16021 atgctgtagg cttgtcatac acatcgaata cactcaatgc cggagcatca ttaccgacct
    16081 gagtatagga agctcttaac ttaccatagt tgagccaatc ctgttttaat atttcagaga
    16141 atataaatcc taaggataca gaaggatagt tgtatgtatt gttagctttt ggcaatgtag
    16201 ttgaacggtc ccttctaaat gttccatcca agaacaaaaa cttattgaac ccaatagtcg
    16261 cagtagcaaa caaaccatca acaccaacag gaacatatcc ttctgatggt ggagtcggtg
    16321 tctgaagtga gttggcaata gaatacaaac catcaacgac caggtctgta ttggtgcttg
    16381 ctgagatctg atacagataa gatcttctta tgttggtacc tgctgtgatg tccagagaga
    16441 tggcctctga aagttccttg ttgtaattgg caaatacatc atagttgtat tccttatagc
    16501 ttctgttggt tacactgtaa gatccaagat ccacggaaga tttattgttt ctctcttcga
    16561 tctggtcaaa tgaaatatca ccacctgccc taccaaccaa tgaaaggccc ggcataatgg
    16621 tgtatgtcgc catgatgttt ccaaaatatc tgtttcttga atccgtagta taatttttat
    16681 accttgcaaa gtatgggtta tcccagtaaa tggggttctc ggctgtaata tctgcccagt
    16741 tccaggtagc attcttttca tgcaattcat atgctgcttt caagtcagat acatctacgt
    16801 tggtttggta ccactgacgg aacatggtgt ttgggtttct gttaccatat cctataccct
    16861 gacgaccggt tgcagcttca tttgaaaagt tgaaattgga actgatctta aacttggaac
    16921 ttgcctccaa tgatcctcca atactaaaca tgtttttata aaggctgctg ttaggcatga
    16981 cacctttttc atcattgcgt gtatagccaa ctttgaaagt tgatgtacta ccaccaccct
    17041 caatacttat actctgattg ctcgaaaatg cagtctcata aaactcagct acatccgttg
    17101 gtgcggctac ccatggagac tttttcttat aattcgggtt caaaggatcg actgacctcc
    17161 agtcataaac caagaagtta ggatcgaact tggcaccgta tgatgcatct tccgtaaatg
    17221 gaacaaacgg ttcttccacg ccgggagaga aaatatctga tccgaggaat cctgatccgc
    17281 cattataaga atagaaatcc ggaccataac ctgcaccata ctgatcctga tgtgtcacaa
    17341 aggtagaacg gtcgatttta ccccaagaca aaccggaatt caaagtaaca tcaagcctgt
    17401 ctttctttcc tttcttggtt gtgatgataa ttacaccatt cgctccacgg ctaccgtaaa
    17461 gcgcagcagc agcacctttc aaaactgtta tactttcgat atcacttgga ttgatgtcag
    17521 cagcactgga accatagtca tacccttctc ttccctgagc tgtggaagaa ctggtcaaag
    17581 tctggttggc agcaggaaca ccgtctataa caaacagcgc ctggttatta cctgtaatgg
    17641 acttataacc cctgagtacc acgttggttg aacctcccat ggtgttaccg gttctaatgt
    17701 caagaccggc tactttactg ttaagtgcac ttacaaagtc attacttctg acgacattaa
    17761 cttcgtcacc ggttactttt tgagctgaaa cagaaagttc atttctcttt ctttctaaac
    17821 cgtatgcggt cacaaccact tcatccaata ttcttttatc ggtttccaaa gatacactaa
    17881 cagtattgga tacgccatcc aacaccactt ccttagttac atatcccgtg taggaaacta
    17941 ccagggttgc ttgaccggaa ggtacccgaa gactaaactt accatcgaca tcggtaactg
    18001 tcccatcctg agttccttta atcaaaacac ttgcccctac aagcggctcg gatttatcat
    18061 ccgtaaccat acctgaaatg gttcgctgtc catatgaaac aacaaaacca aggacaagca
    18121 aaaatagcgt aaacattgtt tttttcatgc ctgaactttt tgattattta attaatgaat
    18181 tacaatatga ataccaaatg taataaactt aatacatagc gtgcatactt tttttgggat
    18241 ttttttaaca aatttattac aataaatcta aaaacaattg aaaaacaatt gaaaatactg
    18301 tttttctttc atatgacaaa acggatttgg cctactttgt gttaaaaata caatatcaag
    18361 tcgtgatttt aaggaatgtt tatttaacaa gctgatatat ggattcaatg aaatgaatag
    18421 ctaccgtgca attgccgacc ggcattagct cggcagcagt ggtccgagac ctattatgtg
    18481 tggcgtcctt atcccaaata atgttcggga tgaatgagcc gatccttata catctgtata
    18541 gcatttgaat agccaaaata catagcatcg acaatcaggg catggccgat agatacctct
    18601 tgaagatgag gcatatgtcg cttgtagaaa gcaagattct tcaggttgag gtcatgacct
    18661 gcattgaccc ctaaaccgat attcaatgct cttcgggaag tgtctgtatg ggcttgcaca
    18721 ccaccattgc cgtccctttc aaaccgatgc gcgtaaggcc cggtatataa ttcaatccta
    18781 tccgcgccaa ccgcctgtgc cttgtcagca ttgtccggtt gcggatcaac gaataaggag
    18841 actctgatgc cggcatcctt gaataccgga actatggctc taagcaaatc ttcgtgcttt
    18901 tcaaaatccc acccatgatc tgaggtcaat gcttccggcg aatctggcac caatgtcacc
    18961 tgatcggctt tattatcaat gacaagtcgg ataaactcct ctgaagggaa gccttcaata
    19021 ttcaattccg tccgaagatg ggccctcaga ataggaatgt catcatatct aatatggcgt
    19081 tgatccggcc tcgggtgcac cgtgattcct tcagccccat aatcctcaag atccaaactc
    19141 atttcgagca gattgggata atctgcacct cttgaattcc ttacaagagc tattttattg
    19201 acatttacac ttaatttggt gtgcatcact taattattca gttattaaaa aatgaaaatg
    19261 gtcaatttcc acctatcttg taattgagtc actaccaaag ctaactctga cagtgtatgc
    19321 tccatatcaa tctatcatgg cctgagccct ccaaatgacc atccgatcct ttatctcccg
    19381 ccatttagat ccaagaagcc tgttcatccc cttatccacg ataaagtgag tggcaatatt
    19441 agccgctccc ttcatccgtc ttctgatgct ttcattggct cgtaaggaaa cactatatcc
    19501 tccttccaca tattcaatat aatgccaata tccagatggg atgaaaaggg attcgcccgg
    19561 atttaaaacc acctcttctc ccttagcatt tcttaaagca ggatacctat catagtccgg
    19621 attgttgata tctatattgc cggctactga atatggcaat tggtagatca accgactttg
    19681 gtccggtgaa aatagtacta ctctttcctt cccgtaaatt tgattgagaa atacatgact
    19741 caggtcaaga ccataatgca tggcgacttt gctacccttc cctccgaaaa acatcgatgg
    19801 taagtcattg taaaacccat ccgtaatatc cggcaggtcg aaatctcctc taagctccgg
    19861 cgcgtattta aagatgttga ataaaaatag tcgcaggtcg cagggttcag agctcaccag
    19921 caaatcgaga aagtccccaa aaagcatgtg cttgtcaggc tccctaaaac ccttgtcagt
    19981 accttcaaat ttgttggtat aaacagaaac tttgagattc ccatattttg atttgaaatt
    20041 ctcaactgtc caattttgca atgctgacca atctttggtt agatccgtaa ataccacagg
    20101 cttcattggc tttagaaaat gttcaatgaa cgaggcacgg tccagtccgg ccactttatc
    20161 caattttgaa atctgcatta tccgattctc atcttttttc aaaatagtga aaaccttagc
    20221 ttaaattatc cgccataaag tttaccccgg aaacacttcc tgccatctta acacaccacc
    20281 aaccaggttt cggacatttg tgtatccgga gttgaccaga gcttctacgg ccatactgct
    20341 ccgcatcccg ctcctacaat gaatgacaat ctcatcgccc ttgttatcat ccagtatatg
    20401 gacatccttg agaaattctc ccaagggaat caatctggct cccaggttaa acacatcata
    20461 ttcatgtggc tcccgaacat caataaagac aaaatcctct ttggcatcca acttcgcctt
    20521 gagttcttct actgttatct ccttcattat ctaataattg atcatgttgc ttattctatg
    20581 gtgtggtatt gcaatggaag catccggcac catctttttg tcaaacaaat ttacatttag
    20641 tgcaataatt ggactaataa aatattgata atcatcagtc ccgacctttg gccggcttaa
    20701 tccataccgt ccttttccct gtcaaatacc tgatccgaac ggcttccgat caatccttca
    20761 atacgctata tcagaaagtg aattgtctaa caaggtcaaa gtcagatccg gcattttaga
    20821 atcagcattc ttcacttttg ggttatattt gataaatttt tataaaaaat ggactaccgg
    20881 actgtattaa acgaaattta ctccgtgggc aaggtaatgg aaggcctcgg caatgtggca
    20941 acctacatcc cggagcttag caatgtcgat ccggataagt ttggtattta tcttttgaca
    21001 ttggaccgcc aaggttatgg tatcggggat gataaggaga gattttccat tcagagtatt
    21061 tccaaaatat ttacagtgtc gatggcaatc agaattctgg gggaatctgt ttggaaacga
    21121 gtcggggtag agccttcggg aagttcattc aattcgatgg ttcagctcga atacgaaaaa
    21181 gggtacccgc gcaatccgct gatcaatccc ggtgcacttg tgattgcaga tatgttgata
    21241 agcaacctgg ctaacccaaa ggaagattta cttgactttg tgcgaatggt ctccggctat
    21301 gaatccataa catttgacga gaaagtggca gagtccgaaa aaaggacagg attccggaat
    21361 gcagcaatgg cgtacatgtt gaaagccttc aacaatctgg ataacgatat agatacagtg
    21421 ctggatttct attttcatca gtgcagtatc accatgtctt gtaaagaact ggccagcgcc
    21481 ttcttttact ttgccaataa aggatatacc tatgacaatt ctattcagat cctgacggaa
    21541 agcaaggtca aaagactcaa tgccctgatg cagacgtgtg gtttctatga cgaatcaggt
    21601 gaatttgcct acaaggtggg gttgccggga aagagcggcg tcggaggcgg aatcatcgcc
    21661 atataccccg gcaggtatac agttgcaacc tggagtccgc gcctcaacag caaaggcaat
    21721 tccgttcgag gaatgaaggc actggaaatg ctgacaacct tcacgaccga atctattttt
    21781 taaatccgga ggtcttctgc agtaaagcct atggaaattc aatgatttca gtcaaaaaag
    21841 acaagatttg ataactattt taaagtgcat taggttatat ttgtagaaca acaaacaatt
    21901 atttattaat ctcaatctca tgaagacaat acttgtaccg acagatttca gtgaaacggc
    21961 cgaaaaggcc ttaaatgcag cagtccatat tggtggtttt cagcaaacag atttggtctt
    22021 gatgcacacc tctcttccta cagaaggggt caatagtaat gtatatgcag ccatttatat
    22081 ccatgaatat cttgaaacca agcgaaagaa tttgaattta ttggcggata aggttcgcga
    22141 aacaggatat aaagggaagg tcaagacaga taataaggtt gattttacgg ttccctccat
    22201 agtcaatgcc gcagaagagt ttgacgccga tcttgtggtg atgggatcaa caggctcaag
    22261 tggtttggca ggtgtttttc ttggaagtat agccggtggt gtgctgtcaa aaatagaaag
    22321 gccgctcttc ctcattacaa aagactttac ccttccggaa gggggagact tgttgatcgg
    22381 tacagattat aagacacgac ctgacaagaa ttcgctggaa atcctccgtt tcttcaaaga
    22441 acaactcaat ttttcattga atcttgtcca tatttcccat ggggaagagg tttcaatcaa
    22501 caaggaggca tttgtcaaca atcattttga tggaatgatc gattcccatg ttgatgttcc
    22561 gggggcaaag gtggaaggag cattgatgaa aatcaaggaa aaataccaaa atgctgtatt
    22621 gtgcaccatt acaagaaaaa gaagctggtt tgagagcttg ttttcacata gtgtatcccg
    22681 ctcgcttacg cactattccg atattccatt gctttgcttg caatcagaat aggcaggtcc
    22741 ggaatacaat gttcacgatc aaggtaagcc atagcatttt ttatccatct ttggttttcg
    22801 acttacacca aagtacgaat tgtataatgg ttgctcaact gaagcaactg agatataaat
    22861 tgaagcattt ggcctccggt gattggattg tggcgccgga aataccacta aaggttctga
    22921 aatacttaat aaatatcagt gtgccaatat cccatgaact tcctttcaat aaagatatgg
    22981 ggacgtcaga aactttagtt aaataaaaca taataaaata gtttaattaa gcacgataca
    23041 tatcttcgtc tcgtgtaatc aaattgagaa atccggtatg aaaaaactga ttttattttt
    23101 tttattagca agtactactt tatatggtca ggatgcgtcc ggggaatata ggcatgaaat
    23161 caagctaaat gggcttaacc ttgttcttgg caacccggaa ttggattatg aatatctgat
    23221 aaacagcaac tccggcgcag gtctgtatgc aaatgccaat atagacagga atcatatctg
    23281 gccatttgtt tatatggccg gtgcctatta ccggttatat ttaggaggta agcatgccaa
    23341 cgggtttttc tttgaaggag gattcaacgc aatcggtttt gatgatacag aatttggtaa
    23401 aaataaagtg tcaaacggat gggggccatc ctttggaatt ggaggaaagt tcctgatcaa
    23461 aaaaacgatt gtcatggaat tttttggagg cctgggtcgt aatttcaaag ctacagatga
    23521 gtacaacaat tatgatattc ctgtattccc gagatggggc atctcaatag gtaaaaggtt
    23581 ctgacccaaa accccggatg ttttgaatta taatattgaa ataagtggag tcagagtacg
    23641 ggtagaagtg cgatatgggc ctacacgcaa cattcgttta ttctatagaa acaataccgc
    23701 aaggctaact gttcccaagg gtttacgaga aaaagacctt ataagagcta aaggcaagct
    23761 gcatgaatgg ttgatcaacc tggcaacggc caagccgcat ctctttgtat ccgatgaata
    23821 cagagctttg acgaaagaaa aaatcattct gctcggacaa tgttaccatt tgaaattcaa
    23881 ggcaactcaa tcaaaccgga tcactattca ccggaaagag gatacccttg cgatggaagg
    23941 aacggacgaa tttttcagag accctgagca aatgtcagat ttcatacctc ggctgttcaa
    24001 caaggtgttt gcccgtgaca tcgaacaacg ggtcagagca atcaatgatc aaaccctcag
    24061 agccaacatt ggacgaatca gtcttaaaag cgttgtgtcg cgctgggggt cttgctcacc
    24121 cgacaacaac atcatgattt ccaaccgatt actattagca ccaacagaaa tcctggatca
    24181 tgtcatcatt cacgaattgg cccacacggt acatagagat cactcccgac agttttggcg
    24241 agtagtcgct caatatgatc ctgccatgaa agagaaaaat gactggctca aacaaaacgg
    24301 acatactttg aactattgat gtcaaattgc ttcccaactt ttcggtggtt gtggcaatga
    24361 taagcatgac gtcccttact tgtgggcatt cggcaatgta ttccatcaac aggaacaaag
    24421 cttaattgtg accggcttta gataagagcc aaaggtcagg ctatctctat tgacttcaag
    24481 attgtcataa tgttatatcc gctaaaatga actccaactg cttcaaggct tttcatcatt
    24541 agtctagttg gtgtgtctaa atattacacc ggtggataca cgctattgga ctttttttca
    24601 aataactttt tcccttcaat aaactatttc ggtaaaaatt atctaaccag taaaatagat
    24661 tctatgaaac aaatatttat tggtttgagt ttgacgatac tttcattcat cagatgttcc
    24721 agggaggagg attcaatcaa aaatcccgat gctctttatt ttgattcatt agacatcccg
    24781 gctcttatgt ttattgcaaa agaatatgac ccgggcaaca attgaaaccc gctcagttga
    24841 tcccttaaga atcaggtggc acaaacactt tttgaccaca aaataaaaat tcataccatg
    24901 aaaaagtaca tatttttaat tccaatgatt gccttcttcc tcacttcctg cagcaaggag
    24961 gtggacaaaa tatcctcaac acccaaattg acgttgacgc ttagcccacc tccctttagt
    25021 gtgccggcag gtacaaagtt ttcggccgat gtctccttca atcaatatcc caattcgaca
    25081 tttgacatat ttatgccttc atcaggcaag cccactgctc ttgctatctt catccatggt
    25141 ggaggatttg ttggagggga caagtcttca atatattccc aaggtcaaga tgatattaag
    25201 tcgtaccttg accattccat tgccttcgct acgattaact atcgcttcaa aaacgaaaat
    25261 ccggatagca acatattgat cagcctggca gatatcaagc gctgtatcca gtttatacga
    25321 tatcacgctt cctccttcaa cattgacaaa gaaaagatag cctgttacgg tggttctgca
    25381 ggtggtggtg cttccattta ccttgctttc catccggata tggccgatcc cggcaatatc
    25441 gatcctatca tgaaggaatc tacacggata atcgctgccg ggcacctgac cagccagtgc
    25501 acctatgacc ccatccgcat ggaagccatg tacgcagaat caggaattga cctcttcgcc
    25561 attccaggca tagaacaatc ggtacttgct gactttagag cggattccat cagtcaattc
    25621 tatacagatc cccgacttgt tgcattgaga cacgaacttg aaatgttggg atggatgact
    25681 ccggatgatc cagagttctt tgtcagcaat gaaaatcccg ctgcaccgcc ttccgaccgt
    25741 agaacggcac tacatcatcc cctccagggc aaagcgctcg ataacaaggc aacagaaata
    25801 ggcctagcac atgtcaccca catcccctca ctcaacctgc ttgcacccaa ccatgagaca
    25861 atcagtcaat tcatgatcag aaaattgagt gaatgatgta cctgctcttt gattgcctat
    25921 aatagatatt aaacttttat aaaaaatgga agtcaaaaag aaaaaataca catgaaaaca
    25981 tatgtacttc tcctgttgat gtttccaata ttcaacctaa gtgctcaaag ttattgtaca
    26041 tccggtcaat acagcgaggt cggctatttt aatccggatg aaatcaccat ccgcaccaat
    26101 gtagaatatg gtcgtgccga gacatttcaa ggtatcacca ttcccttacg aatggatatt
    26161 tatatggcct caactgatgt agacaccacg attcaccgtc cgctgattgt cttaattcat
    26221 ggaggcggat tccttaatgg caagaaaaat aactatgcca ccacagcctc cctattggca
    26281 caagctggtt atgttgtcag tagcatcgac tataggttgg gctgggataa tggcacctct
    26341 ccgtgtaccg gctccctgcc ttctttgctc gctgccgaat accgcgctat gcaggatgct
    26401 catgctgcag tcagatacct tatcgaaaac cagcatttat accacataga taccggtgct
    26461 atatttctgg gcggatcgag tgcgggatcc actacagccc ttcagctcgc ctattacagc
    26521 cctgctgaag cctctaccct gagctctacc cttggcggct tgaatgagtc cggcaatgca
    26581 ttcaggcata cattcagagt caggggtgtc ctcaacgaat gtggaggaat tggcgacacc
    26641 tccttcatta agtcagatac gcctattccc atgattgcat tccatggttc agctgaccgg
    26701 atcgtaccta ttgatacggg tcggatactg gattgttata cgccttcccg gtatcatttt
    26761 atagccggct cacgtgcaat ctaccatacc ctgagaaatc ttggagtctg tacagagctg
    26821 aatgtatatc ccggaggccc ccattgtgct tattccaatg acacacttat agcaaagcgt
    26881 acagcgtgtt tcttcaaagg aatattgtgt catgactgtg cctcactcga tcaggaagga
    26941 gaggtgcctg cttcatgtgc aatggtagcc aaagattacc tgatacaaca agaccgtggg
    27001 gtcaagcttt accccaatcc tgcatccgaa aggttgacta tcctgttgat cggtgaaaca
    27061 gaaaccgtag caaaatattc catcctatcc atgtccggaa gaaaccagat gcaaggcatc
    27121 ctgccggaca aacagtcaac aatcgatgtc tccgggcttg cacaaggaat gtattgtctt
    27181 actattgata tgccgggcaa aacaatcact accaaactga tcaaactata gtgaatagaa
    27241 tgtcacttgc ccaaaatact ttgctcatcg aggcatcatt tagcttccgg agcattcttg
    27301 ctaaatggag tgaagtataa gccgatggtc cttgttgttt aacctgacct gaattttgaa
    27361 atgtccgact caatcacaga taatataaga atgtaaaatt ttgagtttga ccacatagga
    27421 caacaacaga taataatctt ataaattgtc agcgagatca attatcaaac attgatttta
    27481 aataaggaaa tcttgtacag caggtgagac aataaacata tggtattgat tatctgcagt
    27541 ttaaaaatcc aatccttgta aaacgaaatg tacaggatgg tcattgagct ttacaatcaa
    27601 cataagatcc tgtatgattc taaaaaaacc ggaaaaatgg ctccggttgc aaaaaggagc
    27661 taacggtttt tcaaaatatt ctaaatgaag atgtaaaaga tgaatgaaaa cgcaataaag
    27721 gaccaagaaa tattattatt atatcgggca aataaaaatt tataaacaaa caataagttt
    27781 ttaaataact tataatttat tgatagttat atgttttggg gcgttttatc aaaatattcc
    27841 atttcaatga tttttatttt acaaaaaata ttttgtttag ttgtaatcaa aaaaatatat
    27901 ttgcttctca ttctaagttt taaaatatca ttgtaaaaat taaaaaagat ttaacatggc
    27961 aaacgcacct aagcagaacg aaaagaaaac caattccggt tctatactgc cctacattgc
    28021 tattccgttg gcttttgtga ttggaattat tttgtacaaa ttcgtatttg gcgcagccag
    28081 ccattttaag aatggcgacc ccaacaacga tccactaccc ggtgattaca tgggtgttat
    28141 atataaaggt ggattcatcg tgcctattct gctcggatta ttatttatta ctattatttt
    28201 ctctattgag cggtttatta cgattatgcg ggcagccggt acgggccctg tagacaagtt
    28261 tgttcgaaat gtacaatatt ctctttcaaa aggggatatc aatggagcca tagctgcttg
    28321 tgataagcaa aagggttctg taggaaatgt ggtgcgctcc gggcttacca cgtacaacaa
    28381 aatgacaagt gagtcgggta tgactaagga acaacgaatt gccgctatcc agaaagaggt
    28441 cgaagaggcc acttctcttg agctccccag ccttgagc
//
LOCUS       DAOQMC010000003        27229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_579_length_27229_cov_4.642968, whole genome shotgun sequence.
ACCESSION   DAOQMC010000003 DAOQMC010000000
VERSION     DAOQMC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 27229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27229
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_579_length_27229_cov_4.642968"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKV31_00275"
     CDS             <1..313
                     /locus_tag="PKV31_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNB91310.1"
                     /translation="KFIFSGDGESPYQEREQWTDGCNLVAIKPGVAITYDRNPITEEA
                     FRKAGFNILHANDFLVQYKSGKLDPWQLENTIITLPSNELSRGRGGSHCLTCPIEREI
                     M"
     gene            329..739
                     /locus_tag="PKV31_00280"
     CDS             329..739
                     /locus_tag="PKV31_00280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB91311.1"
                     /translation="MYEYSHSLRVRYGETDQMSYVYYGNYALYYEVGRVETMRSLGLS
                     YAVMENEVGVMMPVMRMDIEFLRPAFYDELLTVKTSIPELPDRDIIFQSEIFNGKGKI
                     VNRGHVTLCFVSKETKKRISAPELLLDKLRQFYV"
     gene            732..1682
                     /locus_tag="PKV31_00285"
     CDS             732..1682
                     /locus_tag="PKV31_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNB91312.1"
                     /translation="MFNRKSDSRLAAFMREQTVYFELIDWSRIHTLPGLRGQSIYRIF
                     SELGVHLKRSNIALKASAISFSFMVASFPFMIFLLTLLPYFHIQNAVDVLKSNIGNLL
                     PTNVENTLFGLIEDTIKPRGSLLSIGFISALFFASNGVLNLMSAFDQSERAKTLEINW
                     VKKRMKAILLTILLFLLLVVTIIAFVLANQILQIIGLYINIDYITRGLILAARWVPVF
                     FLMYGLLTLIYRLGPSIKRKIPFFTIGTYMTTFLCILVSLLISYFVNNFSNYNKIYGS
                     LGTIAALMLWLQTNMILIFVGYELNLIINEFPRRKRSKAS"
     gene            complement(1677..3458)
                     /locus_tag="PKV31_00290"
     CDS             complement(1677..3458)
                     /locus_tag="PKV31_00290"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNB91313.1"
                     /translation="MHTNDKLKALREEMLVHGVDAMIIPTGDPHQNEYLPEFYKSRAW
                     VSGFTGSAGTAVVTKDHAGVWTDSRYFLQAEIQFADSEFELHKLVIQGAPEYADWLAD
                     TLGAKSVVGFDANVMPLGLVDTLIEKFEAKDIQLKTDFEPFGKIWKDRPGLSREPIFE
                     HDVKYAGRSRQEKIASIREEMKKHGADYYLVTALDDIAWLLNLRGRDIECNPVFISYV
                     LFSPEKVYLFIDGNKVNAELQQSLEDDNIAIKSYDAIRHYVAEIDKQSSILISPASLS
                     YGLYKKIGTDTIIRKDSPIMHAKSLKNEVEQRHVRHAMIKDGRALAHFFIWLEEALGQ
                     GETPTEYDLTRKLIEFRSQQEGYYGESFDAIIGYKGNGAIIHYKPEEETAAAVRPNGV
                     LLVDSGGQYNDGTTDITRTIALGPVSDEIKDHYTRVLKGHIAVADVIFPKGTRGIQLD
                     TLARQFLWQTGITYNHGTGHGVGFFLNVHEPPQGIANGLGERGVTVILPGTLTSNEPG
                     YYKKDSHGIRTENLVICHEVEGNPDFLYFETVTLFPIDKTMIDISIMSPSETKWLNDY
                     HQKVYRELAPGLDDKARKWLEAKCTAL"
     gene            4101..5588
                     /gene="rpoN"
                     /locus_tag="PKV31_00295"
     CDS             4101..5588
                     /gene="rpoN"
                     /locus_tag="PKV31_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNB91314.1"
                     /translation="MIRQSLSQKLTQKMSPRQIQLMKLLQIPTFELEQRIKEEIESNP
                     ALEEGNNDTYEESGYSDDDMHPGDDIRMDMDDYFPDYSEDGSDYTSPADGYDPDNKQE
                     RQIASHESNLQDYLLGQLELVDFEDETDKIIGQQIIGSIDEDGYLRREPIAITDDLLF
                     SSNMSISEEQVVKVLTVIQGFDPKGVGARTLQEALLIQIRSKLNNTSELDEYKLKDLQ
                     AAEIILRDYFNEFSKKHFSKLVQNLEIDEDDLKAAYEEILKLNPKPGSGFSMGGTTSI
                     QYIEPDFIIVNRDGTLELSLNSRNSPDLKVSNGYKSMIRELQRKKKMGKLSKEDRNTA
                     LFVKQKIEAAQTFIESIQRRQSTLYNTMHAIMMYQYDYFLTGDTQYLKPLILKDIAEE
                     IGMDISTVSRVANSKYVQTEFGTKLLKEFFSEKVQNNDGEDVTTREVKQILSSIIEKE
                     DKSNPLSDEALMELLDKTGISLSRRTISKYREKMNIPVARLRKEL"
     gene            5599..6531
                     /locus_tag="PKV31_00300"
     CDS             5599..6531
                     /locus_tag="PKV31_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNB91315.1"
                     /translation="MNIKIILVVSVITVLTVFASYKSPDKDLPANEAELGEMLFFDPI
                     LSSDKTVSCGSCHKPEHGFADNIPFSFGVDSTLTTRNTPTVKNVLARTKYFWDGRAVT
                     LEEQALKPIENPDEMNLPIAEAIRRLNSDPFYKKAFKKIFKKKANPLNLSHAIAAYEK
                     TLESNNTAFDRYSKGDDSAMSEAALRGREIFVEKANCFECHFGPDFTQDEMINIGIYD
                     GKRYTDKGLGGITGKPKDNGRFKVPGLRNVANTAPYMHNGMFATLREVIDFYNDPDRI
                     IPEALNVDVRLIKPLNLTEQEKQDLEAFLMALSD"
     gene            complement(6556..7197)
                     /locus_tag="PKV31_00305"
     CDS             complement(6556..7197)
                     /locus_tag="PKV31_00305"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNB91316.1"
                     /translation="MLDDFDVFSLKKYTSLRPGMEAWLPLAPPGRFPLPKTGINYRQS
                     AVAVPLFHYEGNLCTLVLLRSKQENDHHSGQLSLPGGKVEPDDADTISTALREMEEET
                     GIRVDKNRYVAHLTKLAIPVSRFEVDPIIFYLNELPPITLSMEEAVSTHIVSLNFLPI
                     DEIPTTDIFSSGVMLKNIPFLKEINGVPLWGATAMILSELIHWIRVVPQYNKL"
     gene            complement(7207..7995)
                     /locus_tag="PKV31_00310"
     CDS             complement(7207..7995)
                     /locus_tag="PKV31_00310"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNB91317.1"
                     /translation="MTTKISTSLKTAKLKRVNSILVSQPPPPPNTKNAFNDLETKYNL
                     KVDYRAFNHVEGVSEKEFRKLRIRPDEYNCVIFNSKHGIENFFRLAEEMRMKISPDTK
                     YFCLTESVANYLQKFIHYRKRKVFVGNKTMQDISAALMKHKNETFLVPISNLGNKPVT
                     EWLTTTKLKFQEAEMYRTVSSDLSDLSDIKYDMLAFFSPQSLSSLFENFPDFVQEETR
                     IAAFGKSTCEAVEYYKLRLDVAAPQPDAPSMPGAIEKYLLNSNK"
     gene            complement(8017..8919)
                     /locus_tag="PKV31_00315"
     CDS             complement(8017..8919)
                     /locus_tag="PKV31_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNB91318.1"
                     /translation="MKIVTTVIVLISFLFQGEIIAQNPFELRQVQTGATAPNDSVRSP
                     EKEQLDPGNPFEIKHVPANYIIRDTLMVKKPVPITSKVVASQNFLFVTISILLLFLTI
                     LVVLNRGLLANLYRAIFSDSYLKMVYRFQNNSQTLSYGLFYLLFFLNAGLFLLLLLKH
                     FKLSFFNSDILLFFACVGIVVIIYLVRHTTLKFIGLVFPVEKEVNYFNFTILAFNIFL
                     GITLLPINGLISFSNEKISDFSLYAGVILIGLMYCMRQLRGMFLAANYLTYQKFHFFI
                     YLCTVEIAPVLILARILYTTGVTL"
     gene            complement(9270..11396)
                     /locus_tag="PKV31_00320"
     CDS             complement(9270..11396)
                     /locus_tag="PKV31_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB91319.1"
                     /translation="MSNNPKNIRNIVLLGHSGSGKTTLVENMLFEAKKINRIGSVDDN
                     STTSDYIEIEKERHSSVYSSLMHVNWKDSKINIIDTPGSDDLVGESISSMKVADTAVM
                     LINAKYGVEVGTELIWEYIEKFHLPSVFAVNHLDNSQADFERALAQARERFGSKVVEV
                     QYPLDAGEGFSSIVDALRMVMYKFPSNGGKPSKEKIPDAEMERAKSMHNTLVELAAEN
                     EEGLMERYFEEGTLSEEDLAKGLTIALANQQFFPVFCICSAKNMGSGRLMGFLNDIGP
                     SPADRPAARLESGETLACDPSKETTLFIYKTISEANVGNVSYFKVYSGTLHTSDELIN
                     ADNDTAERFNHIFVTNGKTRTEVEQLTAGDLGVTVKLKNSHTNNTLNTKGINRKIEPI
                     HFPEPRHRTAIVPPDKNSLEKVARALSIVHEEDPTLVVEQSVELKQTILHGQGQLHLD
                     IVKSRIEKTFGVKIEFDTPRIPYRETIRSGVDEVYRHKKQSGGAGQFAEVHMRIEPYY
                     DGMPAPAGLTVRSEDHEKLPWGGTLSYLWCIVGGTIDARFSNAIKKGVLMKMEEGPLT
                     GSRCRDIRVAVFDGKMHPVDSNDMAFQIASTMAFKNAFKRANPKVLEPIYNLEVLVPE
                     EMVGEVMGDLQTRRAIITGMDSENHYQKISARVPLAELHGYSSTLRSLTQGRAKFKNS
                     FADYSETPPDVQKSLIDAHDDKDEGD"
     gene            complement(11965..14700)
                     /locus_tag="PKV31_00325"
     CDS             complement(11965..14700)
                     /locus_tag="PKV31_00325"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNB91320.1"
                     /translation="MADFSFITNAHPNVIEQLYNDYLKDPESIDKEWKAFFAGFDYSL
                     KYDGLSRPATTVPEDLSKEIGVSNLINAFRLRGHLLSSTNPIRRRKNRAPHLDLNDYG
                     LSEADMNRHFRAGDILGLNNATLKEIIQHLKNIYCSNIGFEFAHIENWERRNWLQQKI
                     ENRTPLQDYGLSNDRKHRILEKLNGAVMFEEFLHTKFVGQKRFSLEGGETTIPALDAM
                     VDFGTDRGVEEVIIGMAHRGRLNVLANIMGKTYEQIFTEFEGTVLPDQSFGDGDVKYH
                     LGYSSQVKNSSGREVYLKLVPNPSHLEAVNPVVEGFARAKADIMYKSDYDKILPILIH
                     GDAAAAGQGIVYEVVQMSQLEGYYTGGTLHFVINNQIGFTTDFEDGRSSTYCTASANL
                     VHAPVFHVNGDDPEAVVFAVELAVEYRQLFNSDVYIDMVCYRKHGHNEGDDPKFTQPQ
                     MYDIIAKHPNPREIYLKQLLSRGDINKEEAQRLENVYWSNLQARLDDVKQKPLPYTYQ
                     EPELAWKALRKTFDPKDFLVSPKTGIKKATLEKILKHLQSLPEGFTPLSKVNRLLKSK
                     DELLSSGSMDWALAELSAYGSILLEGKDVRMSGQDVRRGTFSHRHATFRDAETFDTYN
                     RLTGLSEDQGRFMIYNSLLSEFAVLGFEYGYSLANPNSLVLWEAQFGDFANGAQTIMD
                     QFITSGETKWQRMSGLVLLLPHGYEGQGPEHSSARLERFLSMCADYNVTVANLTTPAN
                     FFHVLRRQLARPFRKPLIVMSPKSLLRHPECVSSINDFVTGNGFREVYDESELTDIKS
                     DKFTKIICCTGKIYYDLLAYRRSNKIKDAAIVRVEQLYPYPKNQIDEILGKYKKARIR
                     WVQEEPANMGALTYIRTLMPEGTEFIARKASASTATGFKKTHDKQQAELMVKAFE"
     gene            complement(15470..16582)
                     /locus_tag="PKV31_00330"
     CDS             complement(15470..16582)
                     /locus_tag="PKV31_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNB91321.1"
                     /translation="MKILDILKQEFMENTIGGYLWFVICILAALALKRVIANILINLS
                     IKLLSRHLSSINPEQFKKKLVKPLSFLILVSSIYLAVQYIDFPASWELADSKHFGIRK
                     VIVVLYGLMFGISLTWILIRLADCIGIILEKNYRPQDEKSTALQVIPFATDFIKIVIG
                     VVAGLIILSTLFGINVGTLVAGLGIGGLAVALAAKDTFENLLGSFVIYLDKPFEIGDY
                     IRINEIYCTVEKIGLRSTRLRTLEKSLLTMPNKMLVESMIDNYTLRPFRRVDRIIALS
                     RDTMHEQMITIIAGLKKAIGEHPLTTDEIYINFHEITSFSLEIRIQYYVMTAEWEIYL
                     KAVGEINLIILKIIEDNGSSLANVQGFLAFRNKATD"
     gene            16799..17404
                     /locus_tag="PKV31_00335"
     CDS             16799..17404
                     /locus_tag="PKV31_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNB91322.1"
                     /translation="MKLLKFLLSICFLFFFLSSYAQTTINPRIGLNISHLSGKPSIGE
                     NESRTGFNIGFNLRFGDRFQFETGAHYATHGVGFNDARGKDTLDRAPMHFVKLPLLAN
                     INIVNSEVFRLRVYGGWMNNFLLKVDNNSLLSKEDVNVYTGGIHFGTGLDLTGLTIDL
                     SYEVGMMDFFKGNGLPLVLYPKNTQNNVLTLSVGVQVGGKR"
     gene            complement(17550..18164)
                     /locus_tag="PKV31_00340"
     CDS             complement(17550..18164)
                     /locus_tag="PKV31_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HNB91323.1"
                     /translation="MIRPMPIYSFKGFIPVVDPSAFVHPMATVTGNVIIGKDVYVGPG
                     AALRGDWGEVVIEDGCNVQENCTLHMFPGKTIRLRKNAHIGHGAVIHGADIGENCLVG
                     MNSVVMDQVIVGAGSIIGALTFVHEGMEIPERSLVVGNPARIVKQVSDTMLDWKKKGT
                     RLYQSLPNDLRQYWAEVKPLTKIPDDRPSQEILFENWENIKLDK"
     gene            complement(18287..19072)
                     /locus_tag="PKV31_00345"
     CDS             complement(18287..19072)
                     /locus_tag="PKV31_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB91324.1"
                     /translation="MRINKGSLAIGLIILLFTLFKYYSSTQVNEVTGEKQHIALSVQD
                     EIAMGLQSAPQMAAEFGGLSSNLNNQAIVKKVGQRIVAYSSAANTPYKFDFHLLADPN
                     TVNAFALPGGQIFITDGLLRRLKTEDQLAGVLGHEAGHVVARHSAEKMAEDQLYQGIL
                     NSATIGSGGDMSVAQIGQLVAGSKSMKFGRNQELQSDDLGVRFMIEAGYDPTAMIDVM
                     EILKEASGGRDVPEFQSTHPSPENRVQHIQEAIQKYNRIYQKK"
     gene            19473..20069
                     /locus_tag="PKV31_00350"
     CDS             19473..20069
                     /locus_tag="PKV31_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91325.1"
                     /translation="MTAFRYTFLLYCIILFTFSSQAQSKDGMVLKAGVSSTVFYKDRN
                     AKLIHHASAGNTVGIDGLVYNGKLLIQPGIYFMSFPSSQGPLKGAFRDVLSREAGSYD
                     YGILMPVRLGVDLIDLGAFRLKSSVGMFGIYSPEGIEINTEAFSKTCYVAGGWTLHAG
                     MILKFLTLDVDYYGSLCKPSNGGPLYMQSIAFTAGFYF"
     gene            20199..21785
                     /gene="bshC"
                     /locus_tag="PKV31_00355"
     CDS             20199..21785
                     /gene="bshC"
                     /locus_tag="PKV31_00355"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNB91326.1"
                     /translation="MVCRKIPFDQVGQFSAFDHLYAREHDSFKDLLAYMPDMDSIGKI
                     VEARKKMAFDRKTIHSILIDRYRENGISGREELIDSLLDENTFTVVTAHQPYLFGGPL
                     YFILKILSTVKLAAQAKDAYPQLNFVPVFYIGGEDHDLDECNHVTIFGKRVSWTTSQI
                     GPVGRMTTDDLDTALNEVQEILGNNENAQYLTGLLKQSFAPGTNINRAISRFIDALLK
                     DFPLLILDADSKIGKEAFKGIIREEVMSGVAESLIHPQQELMKNIGLKPQAFVRPINF
                     FYFSPEGRNRIERAGEGFEIVNTSLHFTVEEMECEIDLYPERFSPNVVMRPLYEEFLL
                     PNVAYVGGGGELAYWADRKLLFEHFGVPMPLLVRRDSMMVLDGGSAKRMYKLGVDVAD
                     LFKEPNTLRAEFLARHAEGGISLSRESAQLSELIREIQAKVAGVDPSLKGFVGAETSV
                     FFKTMAHIEARLRKSLSAKHDVELQQLASLQQKFFPDNGLQERVESFLSFFVREGNDF
                     FRKIYECADPLDIQFKILEL"
     gene            complement(21809..22657)
                     /gene="accD"
                     /locus_tag="PKV31_00360"
     CDS             complement(21809..22657)
                     /gene="accD"
                     /locus_tag="PKV31_00360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNB91327.1"
                     /translation="MSWFKRLKEGITTTTKNKKEAPDGLWYKCPICKEASTMKEFREN
                     FYKCPKCDYHSRIGSREYFEIILNPGYTVLFNNIESEDFLHFVDLKPYKDRLVEAKKK
                     TKLDDALSVATGHIGEFNIPLTIACMDFAFIGGSMGSVVGEKIALAIEHSIKHKTALL
                     IISKSGGARMMESAFSLMQLAKTSAMLTLLSDEGLPYISLMTDPTTGGVTASFAMLGD
                     LNIAEPKALIGFAGPRVIRETIKRDLPEGFQTAEATLENGFLDKIVNRKDLRSTLETM
                     LSMLMK"
     gene            22854..23579
                     /gene="dapB"
                     /locus_tag="PKV31_00365"
     CDS             22854..23579
                     /gene="dapB"
                     /locus_tag="PKV31_00365"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNB91328.1"
                     /translation="MKAALIGYGKMGHVIESVLLEKGHSVSFRISEENKGEIHDIRPD
                     NTDVCFEFTGPNSAFDNLTILIQQGLPVVCGTTGWYARMPEIYDLVKKQGTSFVAASN
                     FSIGVNLFFEAARQLSALMKHRGYSVEINETHHIHKKDAPSGTAITLSEQVRAVLEEF
                     EGYQLNSENEDNLIPIFSHRLEDVPGTHELRFGNEIDAISLKHVAFNRNGFALGAVMT
                     GEYIIGRKGIFTIGEILKHTMYS"
     gene            24178..24554
                     /gene="rnpB"
                     /locus_tag="PKV31_00370"
     ncRNA           24178..24554
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKV31_00370"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(24640..25071)
                     /locus_tag="PKV31_00375"
     CDS             complement(24640..25071)
                     /locus_tag="PKV31_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HNB91329.1"
                     /translation="MLTGFKHLHSYFAYLLLAVLIIGILFNFLAFMQGKSYSNTNRKF
                     ALYGLMATHIQVLFGLILYFVSPLGISALSGEAMKDSTSRLYALEHPLMMILAVVLVT
                     IGYSKSKKATADARKFRIQLAFYAVALVFILSRIPWAAWPH"
     gene            complement(25176..25700)
                     /locus_tag="PKV31_00380"
     CDS             complement(25176..25700)
                     /locus_tag="PKV31_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91330.1"
                     /translation="MVKAKLISDTASYKKTKRLLIWTYTGLLTLYLLYNQVLIGYLGF
                     SPLPLLQYLNLAIVTTIIAAILMLLLGKKLASLIGQGKIEMDEKHLSILKAGTIVQTI
                     DLRPEDKVVLNIVNPFDSLATCKEYKGDSPVSFIEVYKNGGSVANRFDFVIESHYMMT
                     RLEKLKANWASMQK"
     gene            complement(25876..26778)
                     /locus_tag="PKV31_00385"
     CDS             complement(25876..26778)
                     /locus_tag="PKV31_00385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNB91331.1"
                     /translation="MSSESVLNSLLILTAILVAIEAIVSSIQGRQFYTVKDTLYNFYL
                     MALNTGLAFLMLGVSIKVLDFFYQYSLPIHLNPFLYWLALLIVLDFAYYVLHVVDHQS
                     RFFWAIHVTHHSSELFNLTTGFRSSVLQPLYRFIYFIPLSLLGFKTIDILFMHSICQT
                     WGVLVHTKYIKKLPAWIEYIFVTPSHHRVHHASNVLYLDRNMGMFLIIWDRIFGTFQA
                     EEEWEKIRYGLTHEQEKVNPANIITHEWSAIWKDMKKSSNWLDKIKYIFYPPGWSHDG
                     SMKTSNQLRAEMYPQNVRDTDPKG"
     gene            complement(26909..>27229)
                     /locus_tag="PKV31_00390"
     CDS             complement(26909..>27229)
                     /locus_tag="PKV31_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNB91332.1"
                     /translation="ASYVEHEDFFRHKWYLACDSQASINDIKKVLDSKLCEINDDYDT
                     ERRAGVLKEPAVEIIPTQWFYDYMERQGKLTGQTKFPRVIKHQAWEQWERYVLEKQGI
                     LSPR"
BASE COUNT         7466 a         5427 c         6016 g         8320 t
ORIGIN      
        1 gaaattcatt ttctcgggcg atggagaatc tccctatcag gaaagggaac agtggacgga
       61 cggctgtaac ttagtagcta tcaagccggg tgtagcgatt acatacgaca ggaaccctat
      121 tactgaagaa gctttcagaa aagccggttt caatattctg catgccaatg actttctggt
      181 tcaatataaa tccggaaagc tggatccatg gcaacttgaa aacacaatca taaccttgcc
      241 ttccaatgaa ttatcaaggg ggaggggagg ctcacattgt ttgacatgcc cgattgaaag
      301 ggagattatg taatgtaaat attctgtctt gtacgagtac agtcattctc tccgggtcag
      361 atatggcgaa accgatcaga tgtcatatgt atattatggc aattacgcct tatattacga
      421 ggtaggacgt gtggagacca tgcgctcact gggtcttagt tatgctgtca tggaaaatga
      481 agtaggggtg atgatgccgg tgatgaggat ggatatcgaa tttcttcgac ccgcctttta
      541 tgatgagctg cttactgtta agacttcaat tcctgaattg cctgatcgag atataatttt
      601 ccaatcggaa atcttcaacg ggaaaggcaa gattgtcaac cgggggcatg tcacgctctg
      661 ctttgtaagt aaggagacca agaaaagaat ctccgctcct gaattacttt tagacaaact
      721 gagacagttc tatgtttaat cggaaaagtg actctcgatt ggctgcattc atgagggaac
      781 aaacggtgta ttttgagttg attgactggt cacggatcca caccttaccc ggattgagag
      841 ggcagagcat ttacaggata ttcagtgagc tgggggtgca tcttaagaga tccaatatcg
      901 cattaaaagc aagtgccata tcattcagct ttatggtagc atcctttccc ttcatgatct
      961 ttctactgac cttgttgcct tattttcata tccagaatgc agtagatgta ttgaagtcca
     1021 atattgggaa tcttttgcct accaatgttg agaatacgct attcgggctt attgaagata
     1081 caatcaaacc tcggggcagt ctgttgtcca tcggtttcat cagtgccctc ttctttgcat
     1141 ccaatggtgt tctgaacctg atgagtgctt ttgatcagtc ggagcgtgca aaaactttgg
     1201 aaatcaattg ggtaaaaaaa cgaatgaagg cgattctact tactattctg ttgtttttat
     1261 tattggtggt taccatcatc gccttcgtcc ttgccaatca aatccttcaa atcatcggtc
     1321 tgtatatcaa cattgactat atcacacgcg ggctcatcct tgcggcccga tgggtgccgg
     1381 tctttttcct gatgtatgga cttttaaccc ttatctatcg cctgggtcca tccattaaga
     1441 gaaagatccc tttctttacc attggtacat atatgacgac ctttctgtgt attctggtat
     1501 ccttgctgat tagttacttt gtcaacaatt tttccaatta caataagata tacggatccc
     1561 tgggtacgat agcagcattg atgctctggc tacaaaccaa tatgatcctg atctttgttg
     1621 gctacgaatt gaaccttatc attaacgaat ttccgagaag aaaacgttct aaagcgtcat
     1681 agtgcggtac acttggcttc cagccatttt cttgccttat catctagtcc cggtgccagt
     1741 tcccgatata ctttctgatg gtaatcattg agccacttgg tttctgacgg actcatgata
     1801 ctgatgtcaa tcatcgtctt gtctattggg aacaaggtaa cagtctcgaa atacagaaaa
     1861 tcagggtttc cttctacttc atggcatatc acaagatttt cggtacgtat gccgtggctg
     1921 tcctttttgt aatacccggg ttcgttggat gtcaaagtgc ccgggagaat gacggtcact
     1981 ccacgttctc ccaatccatt ggcgatacct tggggcggct catgtacatt gagaaagaaa
     2041 cctacaccat gacctgtacc atgattatat gtaattcctg tttgccagag gaattgtctg
     2101 gccaacgtat ccagttggat gccccgggtg cctttgggga aaatgacatc agccacagcg
     2161 atatgtcctt tcaataccct tgtataatga tcctttattt cgtcactaac cggaccgagc
     2221 gcgatagtcc tggtaatatc ggtggtgcca tcattgtatt ggccacctga gtctaccaat
     2281 aaaacaccat tcggccggac agccgctgct gtttcctcct ccggcttata gtggatgatt
     2341 gcgccattac ccttgtagcc gataattgcg tcaaaactct caccatagta accttcctgc
     2401 tgactacgga attcgatcag cttgcgggtc agatcatact cagttggagt ttcaccctgc
     2461 cccaatgctt cttcaagcca aataaaaaaa tgtgccaatg cacgtccatc cttgatcatg
     2521 gcatgcctga catgtcgctg ttccacttca ttcttgagag acttggcgtg catgatgggg
     2581 ctatccttcc ggatgatagt atcggtgccg atttttttat agagaccgta tgaaagtgag
     2641 gccggactaa tcaatatgga gctttgctta tctatttcag ctacataatg tctgatggca
     2701 tcataggatt taatggcgat attgtcatct tcaaggcttt gttgtaattc agcattgacc
     2761 ttgttgccat caatgaagag atagactttt tcaggagaga atagaacata ggaaatgaat
     2821 accggattgc actcgatgtc acgaccccga aggttcaaca gccaggcaat atcgtccaaa
     2881 gcagtgacca gatagtaatc tgctccgtgt tttttcattt cttcgcggat agaagcgatt
     2941 ttttcctgcc ttgaccggcc tgcatatttc acgtcatgct cgaagatagg ctctctggac
     3001 aaaccgggcc ggtctttcca tattttgcca aatggttcaa agtcggtttt tagttgaata
     3061 tccttagcct cgaatttctc gatcaacgta tccactaatc ccagcggcat tacattggcg
     3121 tcaaagccta caacactttt ggcgcctagt gtgtcggcaa gccagtctgc atattccggg
     3181 gcaccttgta tcaccagttt gtgtaattca aattcgctgt cggcaaattg tatttcagct
     3241 tgcagaaaat accttgagtc agtccagacc ccggcatggt ccttggttac gacagcagtc
     3301 cccgcagatc cggtaaatcc cgatacccag gcccttgact tgtagaattc aggtaaatat
     3361 tcattctgat gagggtctcc ggtagggatg atcatggcat ccacaccgtg taccagcatt
     3421 tcttctctga gagctttcag cttatcgttg gtatgcatta tgtaaaaaat atttttgatg
     3481 taaattgatt caatgagttg cccaaatata aagaaaatag ggaatacaat tgtttaaatg
     3541 tgcctttagg ttgtttatcc atattccact attcttcttt taataaaagg aaattcgaag
     3601 gtaattgatc acggaatatg caacagtata aaggtattta catggtattc aatattatat
     3661 atattattta ttatttcaac ataatgtaag taccaataaa gtaaggctgt gcaaaattct
     3721 tgccaatgct tttaattaag aatatcctta aaattggaac tcctgatatt ttaactccaa
     3781 ggttattgaa atcgctgctt gtcagtttca cggttgatct tccatcaatc tgatggacat
     3841 aattgcttta aacgtgtgga gggtttcaaa cataaagtag gttttttttg ttctgaagat
     3901 caggaagcag tattttggga tgccttttat gtgaagtcag aattcatttg aattgatagg
     3961 agggtgggta gaaaaggagc agatcagcag gggtaagaaa ggtgaaattg atattaattt
     4021 ttgaacgatt atgtattatt aaaatttata atatctttgt gctggcataa ctttcaaaat
     4081 atagagaaca aacatagaca atgattcgac aatcgttaag tcagaagctc actcaaaaaa
     4141 tgtcgccgag gcagattcaa ttaatgaagt tgcttcagat acctactttt gagcttgagc
     4201 aaaggatcaa ggaggaaata gaatcgaatc ctgcattgga agaagggaac aacgacacgt
     4261 acgaagaatc cggatacagt gatgatgata tgcatccggg ggatgatatc cgcatggata
     4321 tggacgacta ttttccggat tatagtgaag atggatcaga ctatacctct cctgccgatg
     4381 gttatgaccc tgataataag caggagcgac agatcgcttc gcacgaaagc aaccttcagg
     4441 attatttgct gggtcaactg gaactagtgg attttgagga cgaaacagac aagattattg
     4501 gccagcaaat cattggaagt atcgatgagg atggatattt gagacgcgag ccaatcgcca
     4561 ttaccgatga tttgttgttt agttccaata tgtccatttc ggaagagcaa gtggtcaagg
     4621 tccttacagt gattcaaggt tttgatccaa aaggagtggg ggcaaggaca ttgcaggagg
     4681 cattgttgat ccagattagg tcaaagttga ataatacttc tgaattggat gaatacaagt
     4741 taaaggatct tcaggctgcc gaaattatac tccgggacta tttcaatgag ttttccaaaa
     4801 agcacttttc caaactagtg caaaatctgg aaatagatga agatgacctg aaggctgcct
     4861 atgaggaaat tttaaagctc aaccccaaac cgggaagtgg attctctatg ggaggtacta
     4921 ccagcatcca atatattgaa ccggatttta tcattgtcaa tcgggatggt acattggaat
     4981 tgtccctgaa tagccgtaat tctccggatt tgaaggtgag taatgggtat aagtcaatga
     5041 ttcgggagtt gcaacgaaag aaaaagatgg gcaaactgtc caaagaagac aggaatacgg
     5101 ctttgtttgt caagcaaaag attgaggcgg ctcagacatt catcgagtcg attcaaaggc
     5161 ggcaaagcac attatataac accatgcatg ccatcatgat gtaccagtat gattacttcc
     5221 tgaccggaga tacgcaatat ttaaagccct tgatattaaa ggatattgct gaagagatcg
     5281 gtatggatat ctccaccgta tcgcgagtcg ccaatagtaa gtatgtccag acggaattcg
     5341 gaacaaagtt gctgaaggaa ttttttagcg agaaggtgca gaataatgat ggtgaggatg
     5401 taacaaccag agaagtcaag caaatccttt ccagcatcat tgaaaaggag gacaagtcca
     5461 accctttgag tgatgaagcc ttgatggagt tgctggacaa aaccggcatt tccctgtctc
     5521 gccgcaccat atccaagtat cgtgagaaga tgaatattcc ggtagccagg ttgcgtaaag
     5581 aattataaag attttcttat gaatattaaa attatccttg tagtatctgt cataactgta
     5641 cttactgtct ttgcttccta taagtcaccc gataaagatt tacccgccaa tgaagctgag
     5701 ctaggtgaga tgttattttt tgacccgatc ctatcatcgg acaaaactgt aagttgcggc
     5761 tcctgtcata agcctgagca tggttttgcg gataatattc ccttcagttt tggggtagat
     5821 agtactttga cgacacgcaa tacgccgacg gtgaaaaatg ttttggcaag aacaaaatat
     5881 ttctgggatg gccgcgctgt aacactggaa gagcaggctc tcaaaccgat agagaatccc
     5941 gatgaaatga atctgccaat tgcagaagct atcaggcgat tgaattcaga tccgttttat
     6001 aagaaagctt tcaagaagat tttcaaaaag aaagcaaatc ctctgaatct gtctcatgcc
     6061 atcgccgctt atgaaaagac gcttgaaagt aacaatacag cttttgatcg ttattctaaa
     6121 ggtgatgatt cagcgatgtc ggaagcggca ctccggggaa gggaaatttt tgtggagaag
     6181 gccaattgtt ttgagtgtca ttttggtccg gactttaccc aggatgagat gattaatatc
     6241 ggtatctatg atggcaaaag gtatactgat aaaggtctgg gtggcattac cggaaaaccg
     6301 aaagacaatg gcagatttaa agtgccgggg ttgcgtaatg tggcgaatac tgcaccctat
     6361 atgcacaatg ggatgtttgc aaccttgcgt gaggtaatcg atttttataa tgatccggat
     6421 cgtataattc ccgaagctct taatgtagat gttcgattaa ttaagcctct caacctcacc
     6481 gaacaggaaa agcaggatct ggaagctttt ctaatggcac tgagtgattg agtgttgtac
     6541 agcccggatg tacatctaaa gtttgttgta ttggggtaca acccgtatcc agtggatcag
     6601 ttctgataaa atcattgctg tagctcccca aagtggaact ccgttaattt ctttcaggaa
     6661 gggaatattc ttgagcatta caccgctgct gaatatatcc gttgtgggaa tttcatcgat
     6721 aggaaggaaa ttgagtgaga ctatatgcgt agagacagcc tcttccatgg ataatgtgat
     6781 gggaggtaat tcattgagat aaaatatgat cggatcaact tcaaatctgc tgaccggaat
     6841 ggccagtttg gtaagatgtg cgacatatct gtttttgtca acccgtattc ccgtttcttc
     6901 ctccatttct ctcaatgcag tagatatggt gtctgcatcg tccggttcga ccttcccgcc
     6961 cggaagactc agctgtccgc tgtgatgatc attttcctgc ttgctcctca ataagaccag
     7021 agtgcagaga ttgccttcat agtgaaataa cggcactgcc accgctgact gtctgtagtt
     7081 gatcccggtc ttgggcaagg ggaatcttcc tggtggtgca agaggaagcc atgcctccat
     7141 tcccggtctt aggctggtgt attttttcag ggaaaaaaca tcaaaatcgt ccagcaatgg
     7201 cgcaaactat ttgttgctat tcaaaaggta cttttcaatg gcgccgggca tagagggtgc
     7261 atcaggttgt ggtgctgcca catccagtct caacttataa tattcaaccg cctcacaggt
     7321 acttttgccg aaagccgcaa tccgagtttc ttcctgtaca aagtctggaa agttttcgaa
     7381 aagactgctt aggctctgag ggctgaaaaa tgccagcatg tcgtacttga tgtcactaag
     7441 atccgaaagg tcgctgctta cagtgcgata catttccgct tcctggaatt tcagtttagt
     7501 agtagtcagc cattctgtga ccggcttatt ccccagattg gatatcggta ccagaaaagt
     7561 ctcattctta tgtttcataa gtgcagctga aatatcctgc atagtcttgt taccgacaaa
     7621 aaccttcctt ttgcgatagt ggataaattt ctgtaggtag ttggctactg attcagtaag
     7681 gcaaaaatat ttggtatcag gagagatctt catcctcatt tcttcggcaa ggcggaaaaa
     7741 attttcgatt ccatgcttgc tgttgaagat gacacaatta tactcgtccg gtcgtatcct
     7801 cagttttctg aattcctttt cagatacacc ttctacatga ttgaaagctc tgtaatccac
     7861 cttcaaattg tattttgtct ccaggtcgtt gaatgcgttt ttggtattgg gtggtggtgg
     7921 gggttgagat accaatatgc tgttaaccct tttcaatttg gctgtcttta atgatgtaga
     7981 aatctttgta gtcatatagt tatactttcc attcgtttat aaagtcactc cagttgtata
     8041 gagtattctg gcgagtatta gtacaggcgc tatttcgacg gtgcaaaggt aaataaaaaa
     8101 atggaatttt tgataagtca aataattcgc agctaaaaac atgccccgca attgacgcat
     8161 acagtacatt aacccaatca gaatcactcc tgcatacaga ctgaaatctg atattttttc
     8221 atttgaaaat gagatcaaac cattgatagg caatagggtt atccctagaa atatattgaa
     8281 tgccagaatc gtgaagttga aataattgac ttccttctca accggaaaca ccaggcctat
     8341 aaattttagt gtcgtgtgtc ggacaagata tattataacc actattccca cacaggcaaa
     8401 gaataggaga atatctgaat tgaaaaaaga tagtttgaag tgttttaaca gtaaaagaag
     8461 gaacaatcct gcatttagga aaaacaacag atagaatagg ccatacgaaa gggtctggct
     8521 gttgttttgg aatcgataca ccatcttgag ataggaatca ctgaagatgg cccgatacag
     8581 attggccaaa agtcctctgt tgagcacaac cagtatcgta agaaagagca gcaggataga
     8641 gatggtaaca aacaggaagt tctggctggc aactaccttg gatgtaatcg gaaccggctt
     8701 tttgaccatt agcgtgtccc ttataatata attggcaggg acatgcttga tttcaaaggg
     8761 attaccggga tctaactgct ctttttccgg ggaacgtaca ctgtcattgg gtgcagttgc
     8821 tcctgtctga acctgtctta gttcaaacgg attctgtgcg ataatttctc cttggaaaag
     8881 gaaggaaatc aggacaatga ccgtggtaac tattttcact cgtttgaaat tctgggcaaa
     8941 ggtacatttt ttttcttttc aaaaaaaaat aaccacagaa ctttggtttc atattgcttt
     9001 aatcatattt caaaatccgg ttgttttgtg taattatata tggggtgatt tatatgtaaa
     9061 aaattatatt tggtattgtt gaatagccgg attgagatca ataagtacaa gcactcaaga
     9121 tggatgaggt gttttgcctc cgatatttca atattctttc cctggccaat agaaatagta
     9181 atcactttca aataaaaaac gcttccgatt atatggaagc gttttgatta gtaaaggaag
     9241 attgtgggca tttacccgga caattatttt caatccccct cgtctttatc atcatgagca
     9301 tcgattagtg acttctgtac atccggtggt gtctcggaat aatctgcaaa cgaattcttg
     9361 aactttgcac gtccttgagt cagagaccgt agggtagaac tgtatccatg cagttcggcc
     9421 aaaggcaccc tggcgctgat cttctggtag tgattttcgc tgtccatacc cgtgatgatg
     9481 gctcgtctgg tctgcaaatc acccataact tctccaacca tttcctccgg aacaagaact
     9541 tctagattat atattggttc gagtactttt ggatttgccc gtttaaaggc atttttgaaa
     9601 gccatggtcg aagcaatctg aaaggccata tcattactgt cgaccgggtg catctttcca
     9661 tcaaatacag ctaccctgat gtcccggcaa cgtgaaccgg taagtggccc ctcttccatc
     9721 ttcatcagta cgcctttttt gattgcattg gagaatcggg catcgatggt gcctccgacg
     9781 atacaccaca ggtaactcaa tgttccgccc catggtaatt tttcatgatc ttcacttctg
     9841 acagtgaggc cggcgggagc aggcatgcca tcgtaataag gctcgatgcg catatgtact
     9901 tctgcaaatt ggcctgcgcc accactttgt tttttatggc gatatacttc atccacaccg
     9961 gagcggatag tttcccggta gggtatccgt ggtgtatcaa attctatttt tactccaaat
    10021 gtcttctcta tcctggattt tacaatatcg agatggagtt gtccctgacc gtgaaggata
    10081 gtttgtttta actcaacgga ttgttcgact accagggtag gatcttcctc atggacaatg
    10141 gacagtgcac gggcaacctt ctccaggcta tttttatccg gtgggacaat agctgtccta
    10201 tggcgtggct cagggaaatg tatcggttct atcttgcgat tgataccttt cgtgttcaac
    10261 gtattattgg tatgtgagtt tttgagcttg acagtgactc ccagatctcc ggctgtcaat
    10321 tgttctactt cagtcctggt tttgccgttg gttacaaata tatggttgaa tctttctgca
    10381 gtgtcattat ccgcattgat caattcgtct gaggtatgca atgtcccact atatactttg
    10441 aagtagctga cattgcccac attggcttcg gagattgttt tataaataaa cagggttgtt
    10501 tccttggaag gatcgcaagc aagggtttct ccactttcca atcttgctgc cgggcggtca
    10561 gccggtgaag gccctatatc attgaggaag cccatcagtc gtccgctacc catatttttg
    10621 gcgctgcata tgcagaatac cgggaagaac tgctgattgg cgagtgcgat agtcaatcct
    10681 ttcgccagat cctcttcact cagggtacct tcctcaaaat atcgctccat taagccttcc
    10741 tcattttctg ctgccagttc caccaaggta ttatgcatcg atttggcacg ttccatttcc
    10801 gcatccggga ttttttcctt ggagggtttg cctccattgg acggaaactt atacatcacc
    10861 atacgcaatg cgtctacaat actggaaaat ccctcacctg catccagtgg gtattgtact
    10921 tcaacaacct tgcttccaaa cctctccctg gcctgagcca gagctctctc aaagtcagcc
    10981 tgagaattgt ccagatggtt gactgcgaaa actgaaggca ggtggaattt ttcaatatac
    11041 tcccagatca gctcggttcc cacttcgacc ccatatttgg cattaatcaa catgacagca
    11101 gtatcagcta ccttcattga ggaaatactc tcgccgacca aatcatccga tccgggtgtg
    11161 tcgatgatgt tgattttact gtctttccag tttacatgca tcagggaaga atataccgaa
    11221 ctatgccttt ccttttctat ttcgatataa tcggaagtcg tcgagttgtc atcgactgaa
    11281 cctatcctgt tgattttctt cgcttcgaaa agcatattct caaccaaggt agtcttcccg
    11341 ctacccgagt gtccaagcag gacaatgttt ctaatgtttt taggattgtt tgacatagac
    11401 agatattata tttaagtaaa acaagtaaaa gccggtgcgc atattctttt tgtagtaatg
    11461 caaaattgca cctggccaat tttagctaaa agtaatcatt tgaattttaa attggtgaaa
    11521 attttgagaa aaaataattt taaagttaat tgtttggaat aaaagccaat tatagaggca
    11581 ttgtatggaa tgaaatttgg agaggcttat attttacttc gtattgaaaa attttattgt
    11641 aaaatattca attgcgaagg caaattaatt gccggctgat atttctcgca gggtgtgttt
    11701 gggcattgaa atattcggtt ggtttttgaa ggttcgccac ttataatagc aaaggatagg
    11761 aaccactgac aagcagccta agtggaagga ctttttattg tgccaaggaa tagaagaagt
    11821 gatacgaata ggtcttttgt aatgtcagga atcatatccc ggcctgataa caaaatccgt
    11881 tgggtctctt tgggataata aaaaagcgga gcaggcagga attaatctct gcactgctcc
    11941 gctctgaatt ttattatcat ctttttattc aaaagccttg accatcaact ccgcttgttg
    12001 cttgtcatgg gtcttcttaa atcctgttgc tgtagatgcg ctcgcttttc tggcaataaa
    12061 ttccgttcct tccggcatca aggtcctgat ataggtgagg gcacccatat tggcaggttc
    12121 ttcctgaacc catcttatcc gggctttttt atatttgccc aatatctcat cgatctggtt
    12181 tttcggatat ggatagagct gttctacacg cacgatggca gcatccttga tcttattgct
    12241 tcttctgtag gcaagcaaat cataatagat ttttcctgta cagcagatga ttttggtaaa
    12301 cttatcggat ttgatatcag tcagttcgct ttcatcatat acttccctga agccgtttcc
    12361 ggtaacaaaa tcattgatgg atgatacgca ctcgggatgt ctgagcaatg attttggaga
    12421 catcacaatc aatggtttgc ggaatggtct ggccaactga cgtcttaaaa cgtggaagaa
    12481 gttagcaggg gtggtcagat tggctacggt aacattgtaa tccgcacaca tactgagaaa
    12541 tctttcgagt cttgcgctac tatgctccgg tccctggcct tcatatccat gtggcagaag
    12601 gagtacgagg ccgctcatcc tttgccattt tgtttcacct gaagtgatga attggtccat
    12661 gattgtctga gcgccattgg caaagtcacc aaattgtgct tcccacagga ccaggctgtt
    12721 cggattggcc agtgaatatc cgtattcaaa gccaagtaca gcaaattctg atagtaggga
    12781 gttgtatatc atgaatctac cctgatcttc agacaggcct gtcaacctgt tgtaggtatc
    12841 aaaagtctca gcatcacgga aggttgcgtg acgatgagag aaggtccccc tccgtacatc
    12901 ctggccactc atcctgacat ccttcccttc aagcaagatc gaaccatagg ccgataactc
    12961 agccagcgcc cagtccattg agccggaaga gagcagttcg tctttgcttt ttaagagtct
    13021 gttgacctta ctcagagggg taaagccctc gggaagactc tgcagatgct tcaatatctt
    13081 ttctaaggtt gcttttttta ttcctgtctt tggagataca aggaaatcct ttggatcaaa
    13141 ggtctttctc agtgctttcc aggccagttc aggctcctga tatgtatagg gaagcggttt
    13201 ttgctttaca tcgtctagtc tggcctgcag gttggaccaa tatacattct caagcctctg
    13261 agcttcttcc ttattgatat caccacgact caacaactgc ttcagataga tttctcttgg
    13321 attaggatgc tttgcgatga tgtcgtacat ctgaggctgg gtaaatttag gatcgtcgcc
    13381 ctcattatga ccgtgcttcc tgtaacacac catatcaata tatacatcgc tgttgaacaa
    13441 ttgtcggtat tcgacggcga gttctactgc aaacacgact gcttccggat cgtcaccgtt
    13501 gacatggaag acaggagcgt ggaccagatt ggcggatgct gtgcaataag tcgatgaacg
    13561 accatcttcg aaatccgtag taaatccaat ctggttgttg atcacgaaat gaagggttcc
    13621 tccggtgtaa taaccttcga gctgactcat ctggaccact tcatatacaa tgccctggcc
    13681 tgccgctgcg gcatcgccgt ggatcaggat cggcaggatt ttgtcgtagt cgctcttgta
    13741 cataatgtct gccttagctc ttgcaaatcc ctcaacgaca ggattgaccg cttctaagtg
    13801 agacggattt ggcacgagct tgagatagac ttctctcccg gatgaatttt taacttgact
    13861 ggaatagccg agatggtatt tcacatcacc atcgccgaaa ctctgatcag gtaatacggt
    13921 accttcaaat tcggtaaata tctgctcata ggtcttaccc atgatatttg ccaggacatt
    13981 gagtctgccc cgatgtgcca tgccgatgat gacttcttct acaccacggt cggttccgaa
    14041 atctaccatt gcatctaatg ccggtatggt tgtttcacct ccttccaggg agaatctttt
    14101 ctgaccaacg aattttgtat gtagaaattc ttcaaacata acggccccat tgagcttttc
    14161 aaggatgcga tgcttcctgt cgttgctcaa tccgtaatcc tgaaggggtg tgcggttttc
    14221 gattttttgt tgcagccagt ttcttctttc ccaattctca atatgggcaa attcgaagcc
    14281 gatattgcta cagtatatat tcttaagatg ctggataatc tccttcaatg tggcattatt
    14341 caaacctaat atgtcaccgg cgcggaagtg tctgttcata tccgcctcac tgagtccata
    14401 gtcgttcagg tcgaggtggg gtgctctgtt cttcctgcga cgaattggat tggtagacga
    14461 aagaaggtgg cctctcaacc tgaaggcatt gatgagattg ctgacaccaa tttccttgga
    14521 aagatcttcc gggacagttg tggcaggcct ggacagccca tcatatttga gggaatagtc
    14581 aaatcctgcg aaaaatgctt tccactcctt gtcgattgat tccgggtcct taagatagtc
    14641 attgtatagc tgttcaatga cattggggtg agcattggta atgaatgaaa aatctgccat
    14701 gacgatagct aaataattat aattttttaa ttaacctgtt acatgatgag cgttgcaaat
    14761 atggcaaata ttattggcat ttttcttgtt tgaacggcta taacatgtat gcccaatgtt
    14821 aaatagggct ttttatctgt ttctcttgat aaatgttatc tgaaatttga caaaaaccaa
    14881 gtgacaatta ttggaatttt aaccttttct ataacttatt ttacaattga ttattgtgtt
    14941 aatagcaaga tatatagtta tatatgaata taataattag gtatttgtca aaatgcttca
    15001 cgcatattcg cggctttatt cgtggctgtt ctatatttta gatttgtcta aaaattaaag
    15061 attcattaat attaattttg gctcaattgt ctggtctcag aaacattggg gaaagagttt
    15121 gtattgaatt tcgggggtct gtttttcatt tttttcactt tgtaaggtta accttcaatg
    15181 ggaggcttgg tattatgaat tttaatgccc tgacagaaac cgttgtcctc ttgtaataat
    15241 ttctgttcgt ataatgttgc ttgttcatta agtctgctaa ccttctgttg gtaggtgaat
    15301 caccaatggt tggtagtata aattgcttag tttttttgtt attgaatttg ttgtgatttt
    15361 tgaatattcc gaacatcttc aacttttctc aacatcaatt gatcaatact tctgaaatgc
    15421 aattcaaagt atggatttgg aaagggttgt tactaccgtg gaatgaggtt taatctgtcg
    15481 ccttgttgcg gaaagctaag aagccttgta cattggccag agaagatcca ttgtcttcaa
    15541 tgatcttcaa aatgattaag ttaatttctc ctacggcttt cagatatatt tcccactcag
    15601 cggtcatcac ataatattga atgcggattt caagggagaa ggaagtaatc tcatgaaaat
    15661 tgatatagat ttcgtcagtt gtaagcgggt gctcgccaat cgcttttttt aggccggcga
    15721 tgatggtaat catctgctca tgcatagtgt ctctggatag agctatgatg cggtcaaccc
    15781 tcctgaaagg tctgagcgtg taattatcaa tcatgctttc aacgagcatt ttattcggca
    15841 ttgtgagcag gcttttttcc agtgtcctaa gccttgtgct ccgtagtccg attttttcca
    15901 ccgtacagta aatttcatta atcctgatgt aatctcctat ttcgaatggc ttgtccagat
    15961 agatcacaaa tgaaccaagg aggttttcaa aagtatcctt tgcagccaag gctacggcga
    16021 ggcctccaat gcccagaccg gcaaccagtg taccaacatt gattccaaat aaagtactga
    16081 gaatgatcag accggcaaca actccgatga cgattttgat aaaatcagta gcaaatggta
    16141 tcacctgtaa ggctgtggac ttttcgtcct gcgggcggta atttttttca aggatgattc
    16201 ctatacaatc tgccagccgg atgagaatcc atgtaagtga tatcccaaac ataagaccat
    16261 atagaacgac aataacctta cggataccaa aatgtttgct atccgccaat tcccaggaag
    16321 caggaaaatc aatatattgt acagctaggt aaatactgct taccaagata agaaagctga
    16381 gaggcttgac cagttttttt ttgaattgtt caggattaat ggaagagaga tgcctgctca
    16441 gtaattttat ggagagattg atcagaatat tggcgatcac cctcttcagg gctaaggcag
    16501 caagaataca tattacaaac cataggtagc caccaatggt gttttccatg aattcctgtt
    16561 tcaaaatgtc caatattttc atacctatac cgaaattaac ttcccttgat atttatggtc
    16621 aaaaatatgt attattgttg tgaatgatat actcggagaa ctttatttat tggccaaagg
    16681 ctgtcgaatc aatggacctg gtatattctg taatgtgatt atttcgatac tgtctctcag
    16741 ccttttttca gactcttgaa aatagttgat atatttgcag tatagtaaat tgaaagaaat
    16801 gaagttattg aaattcttgt tgagtatttg tttcctgttt tttttcctga gttcttatgc
    16861 tcagacaacc atcaatcccc gcattgggct taatataagt cacttgtccg gaaagccttc
    16921 cattggagaa aatgagtcaa ggaccggatt caatatcggc tttaatctgc gatttggtga
    16981 tcgttttcag ttcgaaacag gggctcatta tgcaacacat ggtgttggat tcaatgacgc
    17041 cagaggtaag gatacacttg acagggcacc catgcatttt gttaaattac cacttttggc
    17101 caatattaat atcgtaaata gtgaagtttt caggctgcgg gtttatggtg gatggatgaa
    17161 caattttctg ttgaaggtgg ataataactc tcttctttca aaggaagatg tcaatgtgta
    17221 tacgggagga attcacttcg ggaccggtct tgatctgaca ggattgacaa ttgatctttc
    17281 ctatgaagtg ggaatgatgg actttttcaa gggaaatgga ttgccgcttg tactatatcc
    17341 caagaatacc cagaataatg tgttgacact cagtgtcggg gtacaggttg gaggaaaaag
    17401 ataaaatacg ccggagtttt cgcttacgtg tatattgttg tgattatgag ttgaggggaa
    17461 aaaccggctt tggatattcc caaaaaacat ttgcctagat ggaaacttat tagagtttgt
    17521 tccggtaaag ggctgtttct tgttgacctc tacttatcca acttgatatt ttcccaattt
    17581 tcgaataata tctcctgcga aggtctgtcg tcaggtattt tcgttagggg ttttacttcg
    17641 gcccaatatt gcctgaggtc attgggcagg ctttgataca agcgtgttcc cttttttttc
    17701 cagtcaagca tggtatcgga tacctgttta acgattcgtg caggattgcc taccactaag
    17761 gatctttccg gtatttccat tccttcatgc acgaaagtca atgccccgat gatcgaaccg
    17821 gcccccacta taacctggtc catgacgacg ctgttcatac ccaccaggca attttcaccg
    17881 atatcggctc catggatgac tgctccatgc ccgatgtgtg cattttttct gagtcttatg
    17941 gttttcccgg gaaacatgtg caaagtacag ttttcctgaa cattgcaacc atcttcaatg
    18001 acgacttctc cccaatctcc cctcagggcg gcacccggcc ctacatatac atccttgccg
    18061 atgatgacat tgccggtgac agttgccatc ggatgcacaa aggcgcttgg atcgacaact
    18121 ggaataaagc ctttgaaact atagattggc atagggcgta tcatttaatt ttataggaat
    18181 ttagtttgaa aagcgtaata taaatcccat taaaaaagat taatcaaatt attagatatt
    18241 gctatatgca attatgtgat ttctgaaaaa agaaatttgg tgcctattat tttttctgat
    18301 aaatgcggtt gtatttttgg atcgcttcct gaatatgttg aaccctgttc tccgggctgg
    18361 ggtgggtaga ctggaattct ggaacatccc gacctccact tgcttccttc agtatttcca
    18421 tgacatcgat catggctgtt ggatcatagc cggcttcgat catgaagcga actcctaaat
    18481 catcggattg taattcctga ttgcggccga acttcataga tttacttccg gctaccaact
    18541 gaccgatctg cgcaacactc atatcaccac cggaccctat ggtagctgaa ttcaggatgc
    18601 cttgatacag ctggtcttcg gccatttttt cagcactatg tctggcaacg acatgcccgg
    18661 cttcatgtcc cagtactcct gcgagttgat cctctgtctt cagccttctt agtaagccat
    18721 ctgtaataaa tatctggcct ccgggaagtg caaatgcatt aaccgtattc ggatcagcca
    18781 acagatggaa gtcaaatttg taaggagtat tggctgcaga gctgtatgcc actattcttt
    18841 gacctacttt ttttacaatg gcctggttat tcagattgga agagagtcca ccaaattcag
    18901 ctgccatttg cggggcactt tgaaggccca tggctatttc gtcctgaaca ctcagggcaa
    18961 tatgttgctt ttccccggtc acttcattga cctgggtact ggaatagtat ttaaacaatg
    19021 taaagagcag gataatcaat cctattgcca acgatccttt gttgattctc atgcttcagt
    19081 atttatgacg caaggtagga aaaaagtaac tatttgctca ataattatac cctgaaattg
    19141 tgatcacaca ttgattgggt tgagccgcca gcattgtgcc ggaccttgat ttacagatta
    19201 aagccaggag aaatgcttat tgaatattca aaatccggag tattgcgaca atttattgtc
    19261 gattagagct taaatggttt tcgtatcctt gatttcagat ttgtattaaa ccgtatcagg
    19321 cggatttaca agattagttt caattattat taaaatgatt aaataaatat tacaaaatta
    19381 aataatatat tttatatttg ctgacagatt attatatctc tccctcaagc agctttatta
    19441 cctgatattt atttccaatc aattcaatta ccatgactgc ttttcgttat acctttttgt
    19501 tgtattgtat tattttgttt actttttcat cacaagcaca aagtaaggat ggcatggttt
    19561 tgaaagcggg ggtgtcttcg accgtttttt ataaagatag aaatgcaaag ttgatacatc
    19621 atgcaagtgc cggcaatacg gtgggaatag atggactggt gtacaatggt aagctgctta
    19681 tccagccggg tatttatttt atgagcttcc cttcctctca agggccgctg aaaggcgcct
    19741 tcagggatgt tctctcgagg gaagccggta gctacgatta cggaattttg atgcctgttc
    19801 gattgggtgt tgatttgatc gatctcggag ctttcagatt gaaaagcagt gtagggatgt
    19861 ttgggatcta cagccctgaa ggcatcgaaa ttaatacaga agctttcagt aaaacttgtt
    19921 atgttgcagg aggatggaca ttgcatgcag gaatgattct gaaattcctt accctggatg
    19981 tggattatta tggttcttta tgtaagcctt caaatggagg tccactttat atgcaatcca
    20041 ttgcctttac agcaggtttt tacttctgaa acatttttag tgataatggc catggatttg
    20101 attaacgcgc ttccattgtt gggatgaagt gattttaacg cctaattcat acaactattt
    20161 ggggttagcc ttgttcctgt gggtatcaat attatattat ggtttgccgg aagatcccat
    20221 ttgaccaagt tggccaattc agtgccttcg atcatttata tgccagggag catgattctt
    20281 tcaaagattt gctggcatat atgccggata tggactctat tggtaagatc gtcgaggcca
    20341 ggaagaaaat ggcctttgat cgaaagacaa ttcattctat tttgattgat cgatatcgtg
    20401 aaaatggtat ctccggccgt gaagaactga tcgattcctt gttggatgaa aatacattta
    20461 cggttgtaac tgcgcatcag ccctatctgt ttggcgggcc gttgtacttc atactgaaga
    20521 ttctttctac ggtcaaactg gcagcccagg cgaaggatgc ctaccctcaa ttgaattttg
    20581 ttcctgtttt ctacatcggc ggtgaggatc atgacctgga tgaatgcaac catgtaacta
    20641 tttttggaaa aagggtatcc tggacaacca gtcagatagg gcctgtgggc aggatgacta
    20701 cagatgatct ggatacagca ttaaacgaag tgcaggaaat attggggaac aatgaaaatg
    20761 ctcaatatct gaccggcctt ctcaagcaat cattcgctcc cggtactaat atcaatcgcg
    20821 ccatatcccg gtttatcgat gcattgttga aggattttcc cttattaatc ctggatgcag
    20881 acagcaagat aggcaaggag gcctttaagg gcattatccg tgaagaagtg atgtcgggtg
    20941 ttgctgagtc attgatccat ccccaacagg agttgatgaa gaatattggc ctgaagccac
    21001 aagcttttgt acgaccgatc aatttttttt atttcagtcc ggaggggcgc aatcgcattg
    21061 agcgtgctgg tgaaggattt gaaattgtca atacttcgct tcattttacg gtcgaagaga
    21121 tggaatgcga aattgatctt tatccggaac gtttcagccc gaatgtcgtg atgcgtccgc
    21181 tttatgagga attcctgctg cccaatgtag cttatgtcgg gggaggggga gagctagctt
    21241 attgggcgga tcgtaagctg cttttcgagc attttggcgt gcctatgcct ttgcttgtca
    21301 ggcgcgacag tatgatggtg ctggatggtg gttctgcaaa gaggatgtac aaactgggtg
    21361 ttgatgtggc tgaccttttt aaggagccaa ataccttgag agcggagttt ttggcaagac
    21421 atgcggaagg aggcatttca ctgagcaggg aatcagcaca gttaagcgag ttgatccggg
    21481 aaattcaggc gaaggtggct ggtgtcgatc cttcattgaa gggattcgta ggtgctgaga
    21541 caagtgtttt cttcaagacg atggctcaca ttgaagccag gttgcgaaaa tccttatccg
    21601 ctaagcatga tgtcgagcta cagcaactgg catcactcca gcagaaattt tttccagaca
    21661 atggattgca ggaacgtgta gaaagctttc tgagcttctt tgttcgggaa gggaacgatt
    21721 tttttagaaa aatatacgaa tgcgccgatc cgttggacat ccagtttaag atacttgaat
    21781 tataaggtga aatttaaaat gttgagcctt atttcatcag catactaagc atagtctcca
    21841 aggtggaccg cagatccttg cggttgacga ttttatccag aaatccgttt tccagggtgg
    21901 cttctgcagt ctggaagcct tcgggcagat cgcgtttaat ggtctcgcga atgacgcgtg
    21961 gccctgcaaa accgatcaaa gcctttggtt ctgcaatatt caggtcgcca agcatggcaa
    22021 aggatgcagt tacgcctccg gttgttgggt cagtcatcag ggaaatgtat ggaagacctt
    22081 catcggaaag caaggtgagc atggctgatg tctttgccag ttgcatcagc gaaaaggccg
    22141 attccatcat tctggcacca ccgctctttg agatgattaa tagggcagtc ttgtgtttga
    22201 tactgtgttc gatggcgagc gctatttttt ctcctaccac tgaacccatg gagccgccta
    22261 taaaggcaaa atccatacat gctatcgtca aaggaatatt gaattcccct atgtgaccgg
    22321 ttgccacgga tagagcatcg tctagtttgg tctttttctt agcttcaacc agtctgtcct
    22381 tgtagggctt aaggtccacg aaatgaagaa aatcttctga ttcgatattg ttaaataaaa
    22441 ctgtatagcc gggattgagg atgatttcaa aatattccct ggatcctatt ctggagtggt
    22501 agtcgcattt gggacactta tagaaattct ccctgaattc cttcatggtg ctggcttcct
    22561 tacatatcgg acatttatac caaagtccat ccggtgcttc cttcttgttt ttggttgtgg
    22621 tggtgattcc ttcctttaat cttttaaacc agctcatagt tctaactccc tgatgataaa
    22681 tcaattagtt gttgaatgtt gtgtgcaaat gaatggattg gtaccattaa cctttgctgc
    22741 aatccagcaa aagtacaata attttttcta aatggagagg ttggaatttt gaggtttagt
    22801 gaaaagaaga agttagtgat cgcagacttc aatatttgtt ttacctttat aacatgaaag
    22861 cagcattgat tggttatgga aagatggggc acgtcataga gtctgtcctg ttggagaagg
    22921 ggcattcagt ctctttccgg ataagtgaag agaataaagg cgagatccat gatatccggc
    22981 cagacaatac ggatgtttgt ttcgaattca ccggtcccaa ctctgccttt gataatctga
    23041 ccatcctgat ccaacaaggt ctacctgtgg tttgtgggac gacaggctgg tatgccagga
    23101 tgcctgagat ctacgatttg gttaagaagc agggaacttc ttttgtggcg gcatcgaatt
    23161 tcagcattgg tgtcaacttg ttttttgaag ctgcccgaca attgtctgca ttgatgaagc
    23221 accgcgggta ttcagtcgag atcaacgaaa cccaccacat ccacaaaaag gatgctccga
    23281 gtggtactgc tattacattg agtgaacagg tacgggcggt gttagaggaa tttgaaggtt
    23341 atcaacttaa ctctgagaat gaagacaatc tgataccgat cttttctcat cgacttgagg
    23401 atgttcccgg gacacatgaa ttgaggttcg gcaatgaaat cgatgcaata agcctgaagc
    23461 atgtagcatt caaccgaaat ggatttgccc tgggtgcagt aatgaccggt gagtatatca
    23521 ttggtcggaa aggtatattt accattgggg agatattgaa acataccatg tactcttgac
    23581 aggggatctg attgatgagt tgattattcc tgttgaaact gtttcaatgc acgcgtgaaa
    23641 taaatatctg ttgggggtaa tcatgtactt caaattgact ctcatctttg attgccccta
    23701 cgggctttct gaatgttaaa aagtatgaaa cggcctttta aaaccggatt tttttgcacg
    23761 gtcttggatt tttcagtggt agaaaaagta atgtatattt catgatcaat gattatgttt
    23821 cgggaggatg acattgaaaa aaggtagata ataacgaatg taaactcggc atcccggaac
    23881 cccggattgt ttcactcacg ggacagagca gagctatagt agcatagcta tccggacaag
    23941 tataccaagc atatatggaa tattctataa atcatcttgg tatagcaacc ttaatctgtc
    24001 aacaaatgtg tgatgttctt tttgatgaga tacataggta tctgattttt attattgctc
    24061 aatgcagcgc ttcaataaga gaaggtaggg acaatgtttg ttgttttaca tgctgaaagt
    24121 agatctaccc cggaattttc tacctttgat atgcgttatt aacaaattga agtcaagata
    24181 acattgtact tcgtcgcgct ctgcaatggc agggtgagga aagtccgggc aacatagagc
    24241 agcgtaccac ctaacgggtg ggtcttgcag ccgtgcagga acagagagtg tcgcagaaac
    24301 tataccgcct tgcaaaagca aggtaagggt gaaaatgtgc ggtaagagcg cacacggtgt
    24361 gccggtaacg gtgcgccggg ataaacctta cgcgttgaaa tgccaagtat atccgatgtc
    24421 tacggcatat gccatagagt ggtgtggctc gcaccttgtc ggaggggtgg gcagtaaaga
    24481 gccgtccggt gacggccggc caagataaat gacgaagcta cgtacagaat ccggcttatt
    24541 gcaatgttat cattttgatt ttgaaaaaga aaaagcgctt cttgaagcgc tttttctttt
    24601 atatgtcgga attgaaatta tcctttatta gtgcatttgt caatgtggcc atgctgccca
    24661 agggattctg gaaagtatga atacaagtgc caccgcataa aacgcaagct gaattctgaa
    24721 tttccgtgca tcagctgttg cttttttaga cttgctgtat ccaatagtaa ctaaaacaac
    24781 cgcaaggatc atcatgagtg gatgttccaa tgcatagagt cgggatgtac tgtctttcat
    24841 ggcttcacct gatagcgcag agatgccgag tggcgagaca aaatataata tcaaaccgaa
    24901 tagaacctgt atatgcgtcg ccatcaatcc atataacgca aactttcggt ttgtattgga
    24961 atatgatttt ccttgcataa acgccaggaa attgaaaagg atgcctatga taagcactgc
    25021 aagtagcaga taggcaaaat aggaatgtaa atgtttaaat cctgtcaaca tatttatttt
    25081 tttaaagcat gaagatacag gattatttag acaaattaca ttttgtcttt gataaatgat
    25141 taattaaatt ggaaatgacg aaagatggct gcagactatt tctgcataga tgcccaattt
    25201 gcttttaatt tttccaaccg ggtcatcata taatgagatt ctatgacaaa gtcgaatctg
    25261 ttggctacgc tacctccatt tttataaacc tctataaagg ataccgggct gtccccttta
    25321 tattccttac aagtagctaa tgaatcaaaa gggttgacga tgttcaggac gactttgtcc
    25381 tccggtctca gatcaatggt ttgtacgatt gttccagctt tcagaatgga aagatgtttt
    25441 tcatccattt caattttacc ctgaccgatc agactggcta actttttccc aagcaataac
    25501 atcaggatgg ctgcaatgat agtcgtaact atagcaaggt taagatactg tagtaaaggc
    25561 aacggtgaaa agcccaaata tcctataagt acctgattgt acaacagata caaggtaagc
    25621 agaccggtgt atgtccatat caggagtctt ttggtttttt tataagaggc ggtgtccgaa
    25681 atcagttttg cctttaccat gattacaaaa ttgagaacca aatttacatt gaatcgttta
    25741 aatttacaaa tttttcaaga gataaatgcg tttttctcaa tatcccctgc attgagcatg
    25801 agggacttct atgataaatc caaatcatga agacaatatt tatgttcggg gaagtttaat
    25861 ttaacttcag ggcaatcagc ctttcggatc tgtatctctg acattttgcg gatacatctc
    25921 tgcacgcagt tgattgctgg tcttcatact tccgtcatgg ctccaaccgg gagggtagaa
    25981 gatatatttg attttgtcta accaattact ggactttttc atgtctttcc aaatggcgga
    26041 ccattcgtgg gtgattatgt tagcagggtt gaccttttcc tgctcatgtg tcaggccgta
    26101 ccttattttt tcccattctt cttcagcctg gaaggtgccg aatattctgt cccaaataat
    26161 caggaacatg cccatattac ggtcgaggta caggacattg gaagcgtgat gtactctatg
    26221 gtgagacgga gtaacaaaga tgtattcgat ccatgcagga agtttcttaa tatattttgt
    26281 atgaaccaaa acaccccaag tctggcatat agagtgcata aacaagatgt cgatggtctt
    26341 aaagccgagt aatgacaggg gtatgaagta aatgaagcga tagagtggtt gtaagacgga
    26401 cgatctgaag cctgtcgtga gattaaataa ttcagagctg tgatgagtaa catggattgc
    26461 ccagaaaaat ctgctctgat gatccaccac atgcaggaca taataggcaa agtcgagtac
    26521 aatcagtaga gccagccagt ataaaaaagg attgagatgg attggaaggc tgtattgata
    26581 gaaaaagtca agtactttaa tagatactcc cagcatcaga aaagccagcc ctgtattcag
    26641 tgccatcaag taaaaattat acaaggtatc tttgaccgta tagaattgtc ttccctggat
    26701 tgaagagaca attgcttcta tggctaccaa tatggcagtt agaataagga ggctattcag
    26761 tactgattcg gaagacatat ttttgaaaat tttttaaaag agtccaatta aggttagatt
    26821 gaagtttgcc tataattcag gtgaacttgt gtttgaccat ttttttattg cgcaattctg
    26881 attcttcaca caggtgagtt tctgcagttt accttggcga aagtatgcct tgtttttcaa
    26941 gcacataccg ctcccattgt tcccaagcct gatgtttgat tactcggggg aatttggtct
    27001 gtccggtcag tttgccctga cgttccatat aatcataaaa ccattgtgtt ggaattattt
    27061 ccacagcagg ttctttcagg acgccggccc ttcgttcggt atcatagtca tcgttgatct
    27121 cacacagctt ggagtcgagc acttttttga tgtcattgat tgaagcttgg ctgtcacatg
    27181 ccagatacca tttatggcgg aaaaaatctt cgtgttctac atatgaagc
//
LOCUS       DAOQMC010000004        26377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_609_length_26377_cov_5.342261, whole genome shotgun sequence.
ACCESSION   DAOQMC010000004 DAOQMC010000000
VERSION     DAOQMC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 26377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26377
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_609_length_26377_cov_5.342261"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKV31_00395"
     CDS             <1..426
                     /locus_tag="PKV31_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB91333.1"
                     /translation="GIRHSEYNKNKDAMVKFLQIWVYPNKKNVKPRYDQMTLNRKDRR
                     NKLQQILSPNQDDAGVWIHQDAWFHLGDFDPGQKVTYQIHKNGNGVYLFNLSGTLVVN
                     ETMVNKRDGIGIRDTNNIGIEAQSQAEFLIMEVPMEFSL"
     gene            482..1012
                     /locus_tag="PKV31_00400"
     CDS             482..1012
                     /locus_tag="PKV31_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB91334.1"
                     /translation="MTKTKWVLDPAHSEISFKVRHMMIASVTGNFENFELSALSDGDD
                     FSNASVSLIADVNSVHTKNEQRDIHLKSADFFDSVNYPHIRFEAENVSLEPGENELNG
                     NLTIRNTTLPVTVHIEYTGTGIDPYGNTKAGFSIEAKVKRKDFGLTWNVPLEGGGVLV
                     SEEVKIIGEFQLVKRP"
     gene            complement(1258..7362)
                     /locus_tag="PKV31_00405"
     CDS             complement(1258..7362)
                     /locus_tag="PKV31_00405"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNB91335.1"
                     /translation="MRTISFLFSLIFFVTLTSCFSNLKQKDVGGTGNAISESTFDNEN
                     NENMANLSNPTDPKDFVSVDSLISIRQYKSAEKLLGTLLTKYKSLGQWDGYIRAVMTL
                     ARIKSENENGTKDAIAYLESEVASATFPARQILLNVTAHTYASYLNANRYQLQDVTSG
                     EGLQEDDDFETWSKEKLLEKIDLYYDLSIADEKLRTIPIRDIDLITTQSNADLYRPSL
                     YSFLAHQMLDYLADSRNYIEEPVYKFEIRGSEWFAPYEEFIAMKLEAKDSTSRVFHSL
                     KIYQQLLQHHLSNGNEEALRRIDLERLNFVYEHSVDPLKDRDFKVAIQKQVESGAKQD
                     WKAAFEYRLIELELAIDKYSSDNPDQGDRFLLNKMIERLKKLSSDYPNNKYGIKAQNK
                     IASLQARSVSIQGESVQFPGKPFLLAVQYANLTEAEVNIYKLTREQFFQFQFNRYERN
                     SLQEITGKNLFKTVKIDLPDFADHRSHRVEVKMDGLDQGIYLFVPQKLPDNGEYYSET
                     AVVQFSNLAVMRNNPYGTLDHSTVVIDRMSGMPLEGVKTEIYTYSYNTRNIRLLNESK
                     SDKNGFVTPKVSPDNNYFASFTKGGDQLIDLRQSYPYRFGGRHDRSINYNVFTDRAIY
                     RPGQIIYFKAILYSHDEKSVPHIIPNKKVTVSLRDVNNQEVSKGTFVSNEFGSLHGNF
                     TVPSGVLTGVMSIYIDGQHTKGIQVEEYKRPKFEVKIDTITQAYVLNDKVRISGKALN
                     YAGNAVDGAKGVFTVTRRQIWRYYFWNFDYFRPSFPSNEMIIANGEITTETDGSFTIV
                     FDALADESISKEIKPKFSFDISVDITDINGETQSGSTNVLLGYDPYNTDYNIAGEVEV
                     KDLRNLVVDTKNSAFVSVPTNGRLRIELLTSSNQYLRNKLYAYPERVTISREEYKKLW
                     PYESYLNEADRTTWSVKKTIFDRKIKSGTGPVDALKSVDFEPGDYRIVFDVDGMNPVS
                     NKEALYVSVKADGSKGEFALDRFIFAATDKPSYQVGMVSNQTYKTRNTSGKLLYEFYF
                     QGKLLSREWLNAASALGKTLKINEEYRGGVTVIASAYGFGRAQSVPVQINVPWSNKDL
                     QVKLKTFRDKLEPGAEETWELVVSGPAKEKVLAEVIASMYDASLDVFSRNYFNTIGFP
                     DYHVYYSYSDHHNGLWSLSRHYSMDMQPEIGEQWRNYLDNLLIQWHHTRVYRKMMMSP
                     TMAGGRNFERNAQPEAEMMADAVAGEPTDSNKGAGKEEEPSDQTNHEVVPPSVRSALE
                     ETVFFYPDLHTDKNGDVVLRFRMKEALTRWRLQLFAHTKDLMQATLEEEVTTSKELMV
                     FPNLPRYFRENDKLEVTAKVVNMNGLSGIAHAKLELLDAVTLQPLAGGLIISSAEQTF
                     ALSSGQSSVVNWEVQSPALPVEAVTIRITAWTDSHTDGEENTLPVLSNRMMVTETMPI
                     MVRKGQEKTFTFDAMTKANLSGTVTPYRYTLEYSSNPAWYAVQALPYLMEYPYECTEQ
                     ILSRYYANTLAAYIANSNPKIKAVFERWKNTDALLSNLQKNQELKSALLEETPWVLEA
                     QSEEQQKKNIGLLFDFNRMANEQQGALRKLMERQYQSGGLPWFPGCRENEYVTSYVVE
                     NLGHLLRLGVIKDDDASIYSFLERALNYCDQALVRYYEDIKRNNKNDAKKMDENHLSY
                     WPIQYLYARSMMDLGKNDFQQTEAYRYFFNQSKKYWNKKNQYAQGMIALYLHRAGDKT
                     VPGLIVEGMRQTAFRNPNLGMYWKSDYGYFWYQLPIETQALMIEVFSELTQDEDSVDE
                     MKMWLLLNKQTNNWKTTKATSSAIYALLLQGGAMNLETVYPDITLGGKKLDIPSLKPE
                     PGTGYFKTTYSGKEISKEMQEVKVRNNSRVVNWGGAYYQYFEQLDKIGTFKGTPLTID
                     KSYNIVRKGDRGETLIPLSSGQVVKVGDLIRVRTIIRVDRPMEYVHLKDMRPAGTEPI
                     SNISGYRFKNGLGYYESPKDIANNFFIDYLPKGTYVFEYDIRASLRGNFSTGISTIQC
                     MYAPEFSSHSKGERMEIR"
     gene            8132..8455
                     /locus_tag="PKV31_00410"
     CDS             8132..8455
                     /locus_tag="PKV31_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91336.1"
                     /translation="MMTQRMTDLMKLLENDPNDSFLLFAIAKEYEQMSMPDQAEHYYL
                     TLRKANPSYVGLYYHLARLYEDTGKVEDALNIYDEGIFMANKMRDQHALAELKNAKMN
                     LELMM"
     gene            8726..9802
                     /locus_tag="PKV31_00415"
     CDS             8726..9802
                     /locus_tag="PKV31_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB91337.1"
                     /translation="MVQQFYSLEIDFLEHQTEDSTVIGFNTKEVPEGVFDFKAGQNIT
                     IKYYFDGEEIRRSYSICTCPSESRIHIGVRAIKDGLFSNFALKNLKVGDRLDILPPVG
                     SFTIQGKMATSGRYVFFATGSGITPVISMIKDKLEKDPAATCTLFYSNRSVGSMMFRD
                     ELDGIKNKYSTRFQAVYLFSREQLDSDLLYGRMDGDKVRQLMNIFVADKAGSQYFICG
                     PEEQTIAIRNLLTKELGIPSGQVHFELFHTGDLKPEVKSQEEVTDFKGTAEVTVRIDG
                     KETTFHLAYDKESILDAGIRQGLDLPYSCKGGVCCTCRAQLLEGKVHMDQNYALEDDE
                     VEEGFILTCQSHPRAGKLFVDYDV"
     gene            9809..10399
                     /locus_tag="PKV31_00420"
     CDS             9809..10399
                     /locus_tag="PKV31_00420"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB91338.1"
                     /translation="MKQEGNNPRRDYIILKAAEIMRNQGYAGATMRDIAREVGMEVSS
                     LYNHIRSKHDILATICFSIGDEYITGLDTILDRETAYPEKLSCIIGLHIDLAARHKDL
                     MIVFEEEWKHLQDEDLNKFKSMRAYYEKKLENFFRLGMEQGKFKNMDDRTMLLTFLSS
                     IKWLNYFFKDKNNYDIAVLKREVMALISHGLLADPH"
     gene            10434..11366
                     /gene="paaA"
                     /locus_tag="PKV31_00425"
     CDS             10434..11366
                     /gene="paaA"
                     /locus_tag="PKV31_00425"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HNB91339.1"
                     /translation="MSIAASANHFQDRIDRDEKIEPNDYMPEEYRKTLIRQISQHAHS
                     EIVGMLPEGNWVTRAPTLKRKAILLAKIQDEAGHGLYLYSACETLGVDRDDLIDELHE
                     GKAKYSSIFNYPSLTWADMGAIGWLVDGAAIMNQVPLCRTSFGPYARAMVRICKEESF
                     HQRQGYDILLALCRGSQEQKAMAQDALNRWWWPALMMFGPEDKDSVHTQQSMNWRIKR
                     FTNDELRQRFINMTKPQADFLGLTIPDPDIRWNEEKQGYDFGPINWDEFWQVVKGYGP
                     CNAERIEARKKAWDDGRWVREAAVEYAKKYENQQ"
     gene            11429..11713
                     /gene="paaB"
                     /locus_tag="PKV31_00430"
     CDS             11429..11713
                     /gene="paaB"
                     /locus_tag="PKV31_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNB91340.1"
                     /translation="MSSNWPLWEVFIRSKNGLQHKHVGSLHASDAKTALQNARDVYTR
                     RMEGNSIWVIPSNFITASDPQEDEEFFEPAESKIYRHPTFFELPDEVKHM"
     gene            11725..12486
                     /gene="paaC"
                     /locus_tag="PKV31_00435"
     CDS             11725..12486
                     /gene="paaC"
                     /locus_tag="PKV31_00435"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNB91341.1"
                     /translation="MIENQLPIFRFLVQLGDDALILSQRLSEWCGHGPVLEQDIALTN
                     ISLDILGQARLWYQLACDHTSESMTEDKIAYKRDSAQMTNLTLVEQPNGDWGRTVMRQ
                     YLYDSFKKMYYSRLSETHYASIGEIAHKCKTETNYHYKWSKTWVQRLSMGTEEGHTRM
                     QDALNRLWRFHHELTIPSATDLEISAEGIAPNPADLKDAYLEEISSFLTECTLTIPDD
                     KTVITGGRKGIHTEHLGYILAEMQFLQRAYPDREW"
     gene            12480..12986
                     /gene="paaJ"
                     /locus_tag="PKV31_00440"
     CDS             12480..12986
                     /gene="paaJ"
                     /locus_tag="PKV31_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNB91342.1"
                     /translation="MVMTYTGNDVIALLKEVADPEIPVLSVVDLGIIEKVIVDGNRLY
                     LGILPTYNGCPAIDLIRLMIHEKCYDAGFRDIEIEYLKTPHWTTDRISPEGMNSLREY
                     GIAAPDKSTRIDQLIHGISTIRCPKCDSSHTFLISAFGSTPCKALMQCKDCHEPFEYF
                     KCNGIPND"
     gene            complement(13040..13930)
                     /locus_tag="PKV31_00445"
     CDS             complement(13040..13930)
                     /locus_tag="PKV31_00445"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNB91343.1"
                     /translation="MKHIVVFSKGIEEDQAPIVRELIEKCQSVGIRVSFMKEVYDSVR
                     RWMDLPASMTGIGASDLTGLKDIDFLFSLGGDGTLLEASMLTNDIPIVGINMGRLGFL
                     SSIEKKYVSQTIDRLNSGDYTLEYRSLLHFQSNEGQFESYPYALNDMTIIKRDHSSMI
                     VIHATLNGEFLNSYWADGIIVSTPTGSTGYNLSCGGPILLPTAEDFIITPIAPHNLSV
                     RPIVVPDIAQLEFTIEGRAENFLCTLDSRYEVISPDCKITIRKAPHTIPLVRLPKITF
                     TKTIRDKLQWGRDLRNYKPR"
     gene            complement(13927..15315)
                     /locus_tag="PKV31_00450"
     CDS             complement(13927..15315)
                     /locus_tag="PKV31_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91344.1"
                     /translation="MGRYTFFFFFFVASYCLSGQKVAIDSISISGLEKTREYILIREL
                     NFKKGDSLDLQYLASIFQTNEKRLLNTNLVVEARCNLINLNIEAQSAGVNIHCKEALY
                     IYPAPLLEFIDNDFNKWWRNYNRSLKRLSYGLFLSHINLTGNGDRLTIYGQAGFTKKI
                     SIDYDYPYLNHSNTLGIGFTGFYAANREVAYKTIDNFLVLKRNLDQDLLKRVKLGARM
                     FYRPAIYAKHLVEINYYHFNINDSLTTSYNPDYLNNRSKLSFPEFIYLFRYDTRDVVP
                     YAMKGWSLGLYFSQIGLSRHDIHKSDAGLLLSGFFKITPKLSLEEHTLGRYNLDRGKV
                     PYYFNKAIGYDDIYVRGYDLNVIDGQDYFLLKNIARYKVLGKELDWGKYMVLKAYRKM
                     PLDVYLTGGFDIARVADRFYAYGNPLTNKYVYGYGVGLDVVFYYNKLVRLEVSRNSSR
                     NFGFYLHFDAGL"
     gene            complement(15357..15956)
                     /locus_tag="PKV31_00455"
     CDS             complement(15357..15956)
                     /locus_tag="PKV31_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91345.1"
                     /translation="MFTINIFLRFALIVGSIIAGIVLTTLYGFWYAFPLYLVALILLV
                     GYLMVGTVQSASQLMQAQDMEGAKKRLALTFFPKLLFGPGRSAYYMLKGTFAMQGKDY
                     EGAKGMFKESENSKYTSDNEKAMIYLQQANLAAVKGNYREAATLVKKADELKVTESMI
                     KDQVKQFKQALGKSGQLNMQNRMAMSQRGGSKQRRPKMR"
     gene            complement(16060..17694)
                     /locus_tag="PKV31_00460"
     CDS             complement(16060..17694)
                     /locus_tag="PKV31_00460"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNB91346.1"
                     /translation="MRQKFVIISSALLIAVFLLAWFLGPNWYVVLAIIAALVILGYYD
                     MFQKKHAVMRIFPVLGRLRYLMEELRPKIYQYFIESDIDGRPINRIDRSTIYQRAKKE
                     NDTMPFGTQLDVYTEGYEWLCHSIAPKDFSMLDHDPRVLIGNKDCKQPYSCSIYNISA
                     MSYGSLSANAVEAMNAGAKIGGFAHNTGEGGLTPHHLKHGGDIIWQIGTGYFGCRTAD
                     GNFSPEAFRKKAATPQVKMIEIKISQGAKPGHGGILPASKNTPEIAAIRGIEPHTLVA
                     SPPYHKAFDTPRGLVMFVKQLRELADGKPIGFKLCIGHKSEFIGICKAMMELNIFPDF
                     ITVDGAEGGTGAAPQEFSNYVGAPLLDGLAFVHNMLVGLDIRHHIKILASGKITTGFH
                     IIRAIALGADACYSARAMMMAVGCIQALLCNTNKCPTGVATQDPKLTVGLVVEDKKTR
                     VANFHKGTVESVVELLGASGLDKVSNVTRSHIYRRISFQSMLTYREIFPEYETGSFLK
                     EIPERYKLDFDNASPDHWGIAHIGSWSRQRPLSGAH"
     gene            complement(17775..18146)
                     /locus_tag="PKV31_00465"
     CDS             complement(17775..18146)
                     /locus_tag="PKV31_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNB91347.1"
                     /translation="MIPTINSTDFKTLKTLIANYPVQLKTKEVGNLAIELDRAKIVED
                     EAINPDIIRLGSKFDAQDLDSQKIWTLTLTLPEEADMTKQRISVFSPLGVALIGFSKG
                     DTVEWSLPGGLKKLKILAVKN"
     gene            19105..19788
                     /locus_tag="PKV31_00470"
     CDS             19105..19788
                     /locus_tag="PKV31_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNB91348.1"
                     /translation="MNWFNSFITSRVGQKFVMSLTGLFLISFLLVHLVGNLQLLKDDG
                     GLAFNMYANFMVHNPFIKFISIGLYIGILLHAVQGIALKIKNQKSRGNKGYAVNKFVS
                     KSWYSANMALLGTLILFFILIHMGDFWLKYKFGDVYPMVNIDGQELKNLYWGVNQSFS
                     QLWIVVVYSVAMVVLYFHLLHGFWSAFQTLGIDSHKYTPVIKWVTKVFSFLICLGFLI
                     IPIYCYLNS"
     gene            19839..21815
                     /locus_tag="PKV31_00475"
     CDS             19839..21815
                     /locus_tag="PKV31_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNB91349.1"
                     /translation="MSLDAKIPEGDISQKWSNYKSKVHLVNPANKRNLEVVVVGSGLA
                     GASAAATLAEMGYKVKVFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSDYRLFYDTVK
                     GGDYRSREANVYRLAEVSGNIIDQCVAQGVPFAREYGGMLSNRSFGGVQVQRTFYAAG
                     QTGQQLLLGAYSALERQIGMGKIKMYSRHEMLDVVVIDGKARGIIARDLVTGELERHF
                     GHAVLLCTGGYGNVFFLSTNAMGSNVTAAWKAHKKGAYFANPCYTQIHPTCIPVSGDH
                     QSKLTLMSESLRNDGRIWVPKKKDDARPANQIPEEERDYYLERRYPAFGNLVPRDVAS
                     RAAKERCDEGYGVGTSKMAVYLDFASAIKRYGAIEVTKHGIANPTENDIIHHGKEIIK
                     EKYGNLFDMYKQITGENPYEVPMRIYPAVHYTMGGLWVDYNLMTTVPGMYALGECNFS
                     DHGANRLGASALMQGLADGYFVIPYTIGDYLSSEIRTKAISTDHEAFVAAEADVRARI
                     EKLMNVKGKHSVDHFHKKLGKVMWNKCGMARNAQGLKEAIEEIRQIREEFWSDVRIPG
                     APDEFNPELEKAGRVADFLELSELMCIDALNRNESCGGHFREEFQTEEGEALRDDENF
                     SYVSAWEFKKDGDFSLHKEPLTFEYIKVAQRSYK"
     gene            21867..22613
                     /locus_tag="PKV31_00480"
     CDS             21867..22613
                     /locus_tag="PKV31_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNB91350.1"
                     /translation="MKLKLKVWRQKNGQSEGAFQNYDVDDVSPDMSFLEMFDVLNERL
                     INEGGDPIAFDHDCREGICGMCSMYINGQPHGPNRGVTTCQLHMRSFNDGESITVEPW
                     RAKAFPVIKDLVVDRTAFDRIIAAGGFISSNTGNAQDANSIPIGKEIADKAFESAACI
                     GCGACVAACPNSSASLFTAAKISQLNRLPQGQPERYIRTRDMVHQMDKEGFGACSNTG
                     ACEAECPKEISISHIAKMNRDYIKASLFKD"
     gene            complement(22670..25522)
                     /gene="uvrA"
                     /locus_tag="PKV31_00485"
     CDS             complement(22670..25522)
                     /gene="uvrA"
                     /locus_tag="PKV31_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB91351.1"
                     /translation="MPRTKKQISPEGEPVILYDKLNIVIKGARTNNLKNIDLTIPKHK
                     LIVVTGVSGSGKSSITMDTLYAEGQRRYVESLSSYARQFMTRIKKPEVDYIKGITPAI
                     AIHQKVSSANARSTVGTLTEIFDYLRLLYARIGKTISPITGKVIKKDSVSDIVNFLNN
                     KEEGTRFSILSPFSYNEAEEPLRTVLEKLEQKGFNRVWLDGEVQRIDELVSNLPQGIN
                     KDSENNKIVAYVVVDRIAVRKEDVENFKRIADSIETAIAESNGSCVLLTEEGEMHHFS
                     TRFEENGIGYEEPSVALFNFNSSVGACHVCEGYGMAIGIDESKVIPNKHLSLYDHAIS
                     PWSGDKGKNFYKSMIQNAERLGLSIHTPYHKLSSRELEIIWRGGDGFLGIDDYFKKLQ
                     NTSYKIQDRIALARYRGRTICPECRGKRLRKEALYVKVNGRHIAELAEMDIEQLDEFF
                     REIRLEERDVTIAERILSEINQRLSTMVAIGLGYLTLQRPASTLSGGETQRIHLTRLL
                     GSNLTGSLYLLDEPSVGLHPRDTRNLVTALKKLRDLGNTVIVVEHEEEVIRSADYLVD
                     MGPEAGVLGGEVVFTGNYKDIAQIPVNNSLTIDYLLGRKKIEVPKERRVGRNIIRLDG
                     VYLHNLDGVNVSIPLHSIVCVTGVSGSGKSSLVRGALYPTLVNAIQTRSAHTSNNIVR
                     DLYGDFHTINVVEMVDQQPIGKSSRSNPITYVKAYDYIRDLFTAQPLSKLRGFKPKHF
                     SFNVEGGRCEICKGEGVTIVDMQFLADVSLVCDECKGRRFKNEVLDIKYKDKNIYDVL
                     EMDIREAVDFFADQPEIINRIQPLLDVGLGYVKLGQASSSLSGGEAQRVKLASFLTKD
                     NKVPHTLFIFDEPTTGLHFEDVRKLLIAFNKLIDRGNSIIIVEHNMEVIKSSDYIIDI
                     GPEGGRNGGKILYQGPPEGLKAVKDSHTATFLDLEN"
BASE COUNT         7561 a         5770 c         5585 g         7461 t
ORIGIN      
        1 ggtatccgac acagtgaata caacaaaaac aaggatgcca tggtgaaatt tcttcagatt
       61 tgggtgtacc ctaataagaa gaatgtcaaa ccaagatatg atcaaatgac acttaatagg
      121 aaagatcgcc gcaataagct acaacagata ctttcgccca atcaagacga tgcaggagtt
      181 tggattcatc aggatgcatg gtttcacctc ggcgactttg acccgggcca gaaggttact
      241 tatcagatcc ataaaaatgg taacggtgta tatcttttca atctatcagg caccttggtt
      301 gtcaatgaaa ctatggtcaa caagcgagat ggcataggca tccgggatac caacaacata
      361 ggtatagaag cacaaagtca ggcggaattc ttaataatgg aagttccgat ggagttttcg
      421 ctataagatt aatttccatt gcaatcgata catttttaat aactaaatac atataacaac
      481 tatgacaaaa actaaatggg ttctggaccc ggcgcacagc gagatttcat ttaaggttag
      541 gcacatgatg atagccagtg tgacaggtaa tttcgaaaat tttgaactga gtgccctatc
      601 ggatggggat gatttcagca atgccagcgt ttccctgatt gccgatgtca acagtgtaca
      661 taccaaaaac gaacaaaggg atattcatct caagagtgca gacttctttg actctgtgaa
      721 ttaccctcat attcgatttg aagctgaaaa tgtctcctta gaacccggcg aaaatgaatt
      781 gaatggcaat ctcaccatcc gtaatactac tcttccggta actgtccaca tagaatatac
      841 cggtacggga attgatccgt atggcaatac taaggcgggt tttagtattg aggccaaagt
      901 gaagcggaaa gatttcggtc tcacatggaa cgttccactg gaaggtggag gagtcctggt
      961 aagtgaggag gtaaaaatca taggtgagtt ccaactggtc aaacgaccat gattcactga
     1021 agacgaatga aatgaaagga gcaagaccgc tatgccttgc ccctttcatt tgaccgattt
     1081 ttcataaggc ctgagcacta atcaggtata ttacttggca aatcgattta tatttgctta
     1141 atatcctatc ttttatttta tcgccctaca aacctttcat gtccagttag attttcctgt
     1201 ttacaccaaa gatcaatgtt gaatctcaac ggctaacaca agaccacatc aataccttta
     1261 ccggatctcc attcgttctc ccttgctatg gcttgaaaat tccggagcgt acatgcattg
     1321 tatggtagaa attccggtgg agaaattacc cctaagtgat gcccgaatat catattcaaa
     1381 cacataagtt ccctttggca gataatcgat aaagaaatta tttgcgatat ctttcggcga
     1441 ctcataatat ccaaggccgt tcttgaagcg gtagccgctg atattgctta tcggctctgt
     1501 tcctgcaggg cgcatatcct tcaggtgtac gtattccatg ggcctgtcca ctcgtataat
     1561 ggttcgcaca cggatcaaat cacctacctt tactacctgc ccggaggata aaggtatcaa
     1621 ggtctctccc cggtctcctt tcctgacaat attgtaagac ttatctatag tgaggggagt
     1681 acctttgaaa gtgcctattt tgtccagctg ttcgaagtac tggtagtaag ctcctcccca
     1741 attgaccacc ctggaattgt ttctgacttt gacctcctgc atttccttgc tgatttcttt
     1801 accagaataa gttgtcttga aatagcctgt gcccggctcc ggtttaaggg agggtatatc
     1861 cagtttcttg cctcctaagg taatgtccgg atagactgtt tccagattca tggcacctcc
     1921 ctgaagcaac agggcgtata tggcggaaga tgttgccttg gttgtcttcc agttgttggt
     1981 ctgtttgttg agcaggagcc acatcttcat ctcatcgaca ctgtcctcat cctgagtcaa
     2041 ttctgaaaac acctctatca tcaaagcctg ggtctcaatt ggcaattggt accagaaata
     2101 gccgtagtcg gacttccagt acatacccag gttgggattg cggaaggcag tctgcctcat
     2161 gccttcaacg ataaggcctg gtacggtttt atcgccggcc cgatgcaagt atagtgcaat
     2221 catgccttgt gcatattgat tcttcttgtt ccagtatttt ttagactgat tgaagaaata
     2281 acgataggct tccgtttgct ggaaatcatt tttccccaga tccatcatgg agcgggcata
     2341 taggtattgg attggccaat acgaaagatg attctcatcc atttttttgg catcattctt
     2401 attattccgc ttgatgtctt cataatatcg taccaatgcc tgatcacaat agtttaaagc
     2461 ccgctcaagg aaggaatata tggaagcatc atcgtcctta atgacaccca aacggagtaa
     2521 gtgacccaga ttctcgacta cataggaagt tacatattcg ttttcacggc atccaggaaa
     2581 ccatggcaag ccgccacttt gatattgcct ttccatcaat ttgcgaagcg cgccctgttg
     2641 ttcattagcc attctgttga aatcgaagag taagccgata ttcttctttt gttgttcctc
     2701 gctttgtgcc tccagcaccc atggagtctc ttccagtaag gcagacttca gttcttgatt
     2761 tttctgcagg ttgctcagca gagcgtctgt atttttccat ctttcgaata cagccttgat
     2821 tttaggattg ctattggcta tatatgcggc aagtgtattg gcgtagtaac ggctgagtat
     2881 ttgttccgta cattcgtagg gatactccat gaggtaaggc aacgcctgta cggcatacca
     2941 ggccggattg gacgaatatt ccagtgtata tctatacggt gttactgtac cggatagatt
     3001 agccttagtc atggcatcga aagtaaaagt tttttcctgt cccttgcgta ccatgattgg
     3061 catagtctca gtcaccatca ttctgttgga aagtaccgga agtgtattct cttccccatc
     3121 ggtatgacta tcagtccagg ctgtaatgcg tatggttact gcttcaacag gcaaagcagg
     3181 tgattggact tcccagttga ccacacttga ctggcctgaa cttaaggcaa aagtctgttc
     3241 agcagaacta atgatcaatc ccccggccaa tggctgcaag gtaactgcat ccagaagttc
     3301 gagtttcgcg tgtgcgatac cactaaggcc attcatgttg accaccttgg cggttacctc
     3361 gagcttgtca ttctccctga aatatcgagg taggttggga aataccatta attctttaga
     3421 agtagtgact tcctcctcca aagtggcctg catcaaatct ttggtatgag caaatagctg
     3481 taatctccat cgtgttagcg cttccttcat cctgaatcga aggacaacat ctccgttctt
     3541 atctgtgtgc agatccgggt agaaaaatac ggtttcttcc aaagcagacc gtactgaagg
     3601 cggaacgacc tcgtgatttg tctgatcgga aggctcttcc tctttcccgg cacctttatt
     3661 ggaatctgtc ggctcaccag ccacggcatc ggccatcatt tcagcttccg gttgagcatt
     3721 cctttcaaaa ttccttcccc ctgccattgt cgggctcatc atcatttttc tgtacacacg
     3781 cgtatgatgc cattggatca gcaaattgtc gagatagtta cgccactgct caccgatctc
     3841 aggctgcata tccattgagt agtggcggct taggctccac aatccgttgt gatgatcaga
     3901 ataagaatag tagacatggt aatcaggaaa gccaatcgtg ttaaaataat ttctggaaaa
     3961 cacatcaaga gatgcatcat acatggatgc tatgacttca gccaaaacct tttcctttgc
     4021 cggccccgac acaaccagtt cccaggtttc ttcggccccc ggctctaatt tgtccctaaa
     4081 cgtctttagt ttgacctgca aatccttatt ggaccatggc acattgattt ggaccggtac
     4141 agactgtgcc ctgccaaatc cataagcaga agcaataaca gtgacacctc cccggtactc
     4201 ttcattgatc ttcaatgttt ttcccaaagc tgatgcagca ttcaaccact cgcgagaaag
     4261 aagctttcct tgaaaataga actcataaag caattttccg gaagtattcc tcgtcttgta
     4321 tgtctgatta ctcaccattc ctacctggta gctcggtttg tcagttgcag cgaatataaa
     4381 cctatccagg gcaaattctc cctttgagcc atcggctttc acgcttacat acaacgcctc
     4441 cttgtttgat accggattca taccatcgac atcgaagaca atgcggtaat cacctggttc
     4501 gaaatccact gatttcaatg catcgacagg accggtgccg gatttgattt tccggtcgaa
     4561 aatagttttt ttgacagacc atgttgtgcg atccgcttca ttcagatagc tctcataagg
     4621 ccagagtttt ttgtattctt ccctgcttat tgtcacacgc tccgggtatg cgtatagctt
     4681 attcctcaaa tattgatttg aagaagtgag taattcgata cgcagcctgc cattggtcgg
     4741 tacagagaca aaagcactat tcttcgtgtc caccaccagg tttctgagat cttttacctc
     4801 aacttcaccc gcaatattgt aatctgtatt atatggatca tatcctaaca gaacatttgt
     4861 actgccggat tgggtctctc cattgatatc tgttatatca acggatatgt caaatgaaaa
     4921 cttcggcttg atctctttac taatggattc atccgccagt gcgtcaaata caattgtaaa
     4981 cgaaccgtca gtctctgttg taatctctcc attggcaata atcatttcat tggaggggaa
     5041 agaaggcctg aaataatcaa aattccaaaa gtaataacgc caaatttgtc gtcgtgtaac
     5101 ggtaaatacg ccttttgctc catcaactgc atttcctgcg taattgagtg ccttgccact
     5161 tattcttact ttgtcattga gtacataagc ctgagtgatg gtatctatct ttacttcaaa
     5221 tttaggccgc ttgtattctt caacctgaat tcctttagtg tgttgtccat caatatatat
     5281 gctcatgacg ccggtcaaga caccggaagg tactgtaaaa ttgccatgca aggagccgaa
     5341 ctcattggaa acaaaggttc cttttgatac ctcctgatta ttgacatcac gcagactgac
     5401 agtcaccttt ttatttggaa tgatgtgagg aacagacttc tcatcatggc tgtacaggat
     5461 agctttgaaa tagataatct gtcccggcct gtaaatagcc cggtccgtaa atacattgta
     5521 atttatagac ctgtcgtgcc gaccaccaaa tctataggga taggattgtc ggaggtcaat
     5581 caattgatct ccacctttgg taaatgatgc gaaatagttg ttgtccggtg agactttagg
     5641 tgtgacgaat ccgttcttat cagacttgct ttcattgagt aacctaatgt tacgtgtatt
     5701 gtagctgtat gtatagattt ctgtcttcac accttctaag ggcattccac tcatacggtc
     5761 aatgaccact gtcgaatgat ccaatgtgcc atagggattg tttctcatta ctgccaggtt
     5821 agagaattgc accacagccg tttcactata atattctcca ttgtcgggga gtttctgtgg
     5881 tacaaagagg taaataccct gatcgagccc gtccatcttt acttccacac gatgagaacg
     5941 atggtcagca aagtccggca aatctatctt caccgttttg aagaggttct taccggttat
     6001 ttcctgcaat gaattcctct catatcggtt gaattgaaat tgaaaaaatt gttcacgggt
     6061 caatttgtaa atatttacct cagcctcggt aagattggca tattgcacag ccagcagaaa
     6121 gggtttgccg gggaattgga cactttcccc ttgaatcgat acagagcggg cctgaaggct
     6181 ggcaattttg ttctgcgcct tgatgccgta tttattgttg gggtagtccg aagaaagctt
     6241 tttcaacctt tcaatcattt tgtttagcaa aaaccgatcc ccttgatcag gattatcgga
     6301 agaatattta tcgatggcaa gctccaattc aatcagccta tattcaaagg cagctttcca
     6361 gtcctgttta gctcctgatt caacctgttt ttgtattgcc actttgaagt ccctatcctt
     6421 caggggatcc accgaatgct catatacaaa attcaacctt tccaggtcta ttctccgtaa
     6481 agcctcctca ttgccgttgc tcagatgatg ttgcaacaat tgttggtaga tttttagact
     6541 atgaaatacc cgcgatgtag aatcttttgc ctcgagcttc atggcgataa attcctcata
     6601 tggagcaaac cattcactgc cacggatttc aaatttatat accggttctt caatataatt
     6661 tctgctgtcg gcaagatagt ccagcatctg atgagccaga aaactatata gtgaaggcct
     6721 gtataggtct gcattacttt gggttgtaat caggtcgata tcccggatag gtatggtcct
     6781 gagcttttca tcggctatgg aaaggtcata gtataagtca attttttcca aaagtttttc
     6841 tttagaccag gtctcaaagt catcatcttc ctgcaggccc tcaccggatg ttacatcttg
     6901 aagttggtac cggttggcat tcagatagga agcataggta tgagcggtga cattcagaag
     6961 aatctgacga gccggaaaag tggcggaagc aacctcactt tcaagatatg ctatggcgtc
     7021 cttggtgcca ttttcattct cagactttat cctggccaag gtcatgaccg cgcggatata
     7081 gccatcccac tgtcctaaac tcttgtattt agtcaacaag gtgcctaaca atttttcggc
     7141 actcttatat tgacgtatac tgatgagact gtctactgaa acaaagtctt ttggatcggt
     7201 aggattggat agattggcca tattttcgtt attttcgtta tcaaatgtac tttcagaaat
     7261 agcattaccg gtacctccaa catccttttg cttcagattt gaaaaacaac ttgtcagggt
     7321 gacgaaaaat atcaggctaa ataaaaaact gatagttcgc atggttgtat tattaaggtt
     7381 gaacaatgat tgtccggtta taaatccatt ttcttttcca taacgtaatt ttaagtcata
     7441 aagttattga agaatgaaac tttctcaaaa aacattttaa tttactaaaa aactttttca
     7501 tttaaaaaaa atgaaaggac taccaagctt gatggtgttc actttggcag gccttcctga
     7561 tagattcccg gaaactattc atttgattaa taacaatatc atcgtaggtt aatcagattg
     7621 aaggtacaaa taccaagcgg cattattgca ggaaaatttg ttctgatcaa acttgagcaa
     7681 cactgatttt tatttaaagc gaaaaaagga agacgcagaa ggatttttgc ttcttttatt
     7741 tcattaagtg attcatattc aactcaatac acccatattg agtgtatccg ttcttcaaac
     7801 tggttcataa aactatttgg cgaaattaat caggttgata attactttag tgaaattttc
     7861 atgggtatca agtagtaaac ccctcataat caggtagcaa ttataagaaa tcaggcaagg
     7921 ttaaccagat tcgttacaaa taatatgaga taaaacagaa atgtgattat tatttcttat
     7981 atttacggga acaaggaaaa atagtcattc ggatcaaagg caaagaaaca gattgtgaaa
     8041 caactattca ctaaacgaca aaaaaggtag gtcgctatca tttttgtgtg aatgattaga
     8101 tctgtttatt gttaaagaaa aatgaagaga tatgatgacg caaagaatga ctgatctgat
     8161 gaagcttttg gaaaatgacc ccaatgacag cttccttttg tttgccatag ccaaggaata
     8221 tgaacaaatg agcatgcctg accaggcaga gcattattac ctgacactcc gtaaggccaa
     8281 tccttcctat gtgggacttt attaccacct ggccaggtta tatgaggata ccggaaaggt
     8341 ggaggacgcc ctgaatatat atgacgaagg aatatttatg gcaaataaga tgcgcgacca
     8401 acatgccctt gctgaattaa aaaacgcaaa aatgaacctc gagttgatga tgtagtgatt
     8461 cagctcttct caacttttgc agcattgctg ggttactctt acccggaaat ccaatacatt
     8521 gtggatgttc ctaaacgaca ttttttactg acccggtcat ccaatttctt aatgagtccg
     8581 taaaacgact tttcaattga atccagacaa cctgctttct ctccttaaaa acaatcaaga
     8641 acattttcgc gctaaacttg ttcgacctac gaacaattgt tcgcatatca tcatattttt
     8701 ctatttttgc gcaaaaattc aaccagtggt tcaacagttt tattcactcg aaatagattt
     8761 tctggaacat cagacagaag acagtacagt cataggtttc aataccaagg aagtacctga
     8821 gggcgtattc gattttaaag caggacaaaa catcaccatt aaatattatt tcgatgggga
     8881 ggagatccgc agatcataca gtatctgcac ctgtccttca gagagtcgga tccatatcgg
     8941 agtaagggcc atcaaggacg gtcttttttc caacttcgct cttaaaaatt taaaagtcgg
     9001 tgacagactc gatattcttc ctccggttgg cagtttcacg atacaaggca agatggcaac
     9061 cagcggccgg tatgtatttt ttgccacagg aagcggcatc acaccggtga tttccatgat
     9121 aaaggacaaa ctggaaaaag atccggctgc tacctgtacc ttattttaca gcaaccgttc
     9181 ggtaggttcg atgatgttcc gggatgaact ggacggtatc aagaataaat acagtaccag
     9241 atttcaggct gtatatctgt tcagccgaga acaactggat tcggatctcc tgtacggaag
     9301 gatggatgga gacaaggtga ggcaattgat gaacatcttt gttgccgata aagcaggtag
     9361 ccaatatttt atttgcggac cagaagaaca gaccattgcc atcaggaatc tcctgacaaa
     9421 ggaattgggc ataccttccg gtcaggttca cttcgaactt ttccatacgg gcgacctaaa
     9481 gccggaagta aagtcacagg aggaggttac cgacttcaaa ggtacagccg aggtcacagt
     9541 caggatcgat ggtaaggaaa ccaccttcca tctcgcttat gacaaagaga gcattctgga
     9601 tgccggaata aggcagggcc tggatctccc atattcctgt aagggaggtg tgtgctgtac
     9661 atgcagggca caattgctgg agggcaaggt acatatggat cagaattatg cattggagga
     9721 tgatgaggtt gaagaaggct ttatcctgac ttgccagtca catccgcgtg cagggaaatt
     9781 attcgtcgat tatgatgtat aatggtgcat gaagcaggaa ggtaacaatc cgcgccggga
     9841 ttacatcatc cttaaggcag cagaaatcat gaggaatcag ggttatgccg gtgccaccat
     9901 gcgcgatatt gcccgggaag taggtatgga agtcagcagt ctatacaacc atatccgctc
     9961 taaacatgat atcctcgcta ccatttgctt ttcaatcggg gatgaatata tcacaggtct
    10021 tgacaccatc ctggatcggg aaacagctta tcccgaaaag ctttcctgta taatcgggct
    10081 acatattgat ctggcagcaa ggcacaagga cttgatgatt gtatttgaag aagaatggaa
    10141 acatttgcag gacgaagatt taaataaatt caaaagcatg cgtgcttatt atgaaaaaaa
    10201 gctggaaaac tttttccgat tgggaatgga acagggaaaa tttaaaaaca tggacgaccg
    10261 gaccatgctg ctaaccttcc tttcttccat taaatggctc aattatttct ttaaagacaa
    10321 aaataattac gatattgctg tcctcaaaag agaagtcatg gccttaatca gtcatggatt
    10381 gttggccgac ccgcactaat acaaattaaa attaacaatt caaattaagg aatatgtcaa
    10441 tagctgcatc cgcaaatcat tttcaggaca ggatcgaccg ggatgaaaag atagaaccca
    10501 atgattacat gccggaagaa tacagaaaga cactaatcag acagatttct cagcacgctc
    10561 attcagagat tgtaggtatg ctacctgaag gtaattgggt tacaagggcc cctacattga
    10621 agagaaaggc catcttgctg gccaaaatcc aggatgaagc aggacacgga ctttacctgt
    10681 atagcgcctg cgaaacactg ggtgtggaca gggatgatct aatagatgaa ttgcatgaag
    10741 gaaaggctaa atactccagt atattcaact atccatcgtt aacctgggca gacatgggtg
    10801 ccatcggctg gttggtggat ggtgccgcaa tcatgaacca ggtgcctttg tgtcgtactt
    10861 ccttcggacc ctatgccaga gctatggtta ggatttgtaa ggaggagtcc ttccaccagc
    10921 gacaaggtta tgacatccta cttgctttgt gccgtggatc tcaggagcaa aaagctatgg
    10981 cccaggatgc actcaacaga tggtggtggc cggcactgat gatgtttggc ccggaagata
    11041 aggattctgt gcacacccag caaagcatga attggcggat caagcggttt accaacgatg
    11101 agctgcggca acggtttatc aatatgacaa agccacaggc tgactttctg gggctgacca
    11161 ttcccgatcc agatatcagg tggaatgaag aaaagcaagg ttacgacttt ggcccgatca
    11221 actgggatga attctggcaa gtagtcaaag gttacggtcc gtgtaatgcc gaacgtatcg
    11281 aagcgaggaa aaaagcctgg gatgatggac gttgggtacg tgaagcagcc gtcgaatatg
    11341 ccaaaaaata cgaaaatcaa caataatttt cctgttagaa tatcttcata atcaatcatt
    11401 tgtaaactaa ccttaaaaaa aatatgcgat gagcagcaat tggccacttt gggaagtttt
    11461 tatcagaagc aagaacggac tccaacacaa acatgtcggc agcctgcatg cttcagatgc
    11521 aaaaacggca ttacaaaatg cccgcgatgt atataccaga agaatggaag gcaacagcat
    11581 ctgggttatc ccttccaact ttatcacggc atccgaccct caggaagatg aagaattttt
    11641 cgaaccggcg gaaagtaaga tatacagaca tccgaccttc tttgaactgc cggatgaagt
    11701 caaacacatg taaattcatt agaaatgatc gaaaatcaac ttccaatatt ccgatttctg
    11761 gtacaattgg gtgatgatgc cctcatcctt agccagcgtc tttccgaatg gtgtggccac
    11821 ggacccgtcc tggagcaaga cattgcattg accaatattt cactcgatat actgggacag
    11881 gcacggcttt ggtaccagct ggcctgtgac catacttcag aaagcatgac cgaagataaa
    11941 atcgcctaca aaagagattc agcccagatg accaatctga ctctcgtaga gcaaccaaac
    12001 ggcgactggg gacgaacagt gatgcgacaa tatctttacg atagtttcaa gaaaatgtat
    12061 tattcccggc ttagtgagac tcattatgcg tcgatcggtg aaatagctca caaatgcaag
    12121 acagagacca attatcatta caaatggagc aagacttggg tacagcgact gagcatgggc
    12181 accgaagaag gtcacacgag gatgcaggac gcactcaatc ggctatggcg ctttcatcat
    12241 gagcttacta tcccgagtgc cacagatctg gagatttccg ctgaaggcat agctcccaat
    12301 ccagccgatc tgaaagatgc atatttagaa gaaatatcga gttttctgac tgagtgcacc
    12361 ctgaccatac ctgatgataa aacggttatt acaggaggca ggaagggtat tcataccgaa
    12421 catctgggct acattctggc tgaaatgcaa tttttacaaa gagcttatcc tgaccgggaa
    12481 tggtaatgac atataccggg aacgatgtga tcgctctgct caaagaagta gcagatccgg
    12541 agattcccgt gttgtctgta gtagatcttg gaattataga aaaggtaatt gtagacggta
    12601 atcgtctcta tttgggcatt ctacctacct acaatggttg cccggccatc gatctgataa
    12661 ggttgatgat ccacgaaaaa tgctatgatg cggggtttag agatatcgaa atagaatacc
    12721 tgaagacacc acactggacg acagaccgga taagtccgga aggtatgaat tcgctccggg
    12781 agtacggtat cgcagccccg gacaaatcca ccaggattga ccagctcatc catggaattt
    12841 ctacaataag gtgcccaaaa tgcgattcct cccatacgtt cctgatttcg gcattcggat
    12901 ccacgccatg taaagcactg atgcaatgca aggattgtca tgaaccattt gagtatttca
    12961 aatgcaatgg gataccaaat gattagccgg atttgcaaat gccaatttca atacgaaatt
    13021 caagccttcc gtaaagtcct tatctcggct tataatttcg caaatcacgt ccccactgta
    13081 atttatccct tatcgtcttg gtaaatgtaa tctttggaag cctcacaaga gggatggtat
    13141 ggggtgcctt tcggatcgta atcttacaat cgggagagat gacttcatac ctgctatcca
    13201 gtgtacaaag aaagttctct gctctgcctt cgatggtaaa ttccagctga gcaatatcgg
    13261 gtaccacaat aggccttaca cttaagttgt gcggagcgat aggcgtgatg atgaagtcct
    13321 ccgcagttgg aagcaggatg ggcccgccac aactgagatt atagccggta ctacctgtcg
    13381 gggtggaaac aatgataccg tcggcccaat aagaattgag aaactcccca tttaatgtgg
    13441 cgtgtatcac gatcatcgat gagtgatcac gcttgatgat ggtcatatcg ttgagtgcat
    13501 acgggtagga ttcaaattgg ccttcattgg actgaaagtg cagaagagac ctgtattcaa
    13561 gcgtataatc accggaattg aggcgatcga tagtctggct gacatacttc ttctcaatac
    13621 tggacagaaa acccaatcta cccatattga taccgactat cgggatatca ttggtaagca
    13681 tggacgcttc caacaaagtg ccatccccac ccaaagaaaa caaaaaatcg atgtccttca
    13741 atccggtcaa atccgaggct cctattccgg tcattgaggc aggcaggtcc atccatcgtc
    13801 ggactgagtc ataaacttcc ttcatgaagg aaaccctgat cccgacagat tggcattttt
    13861 caatcaattc cctgacaatc ggagcctgat cttcttcgat tcctttggaa aaaacaacaa
    13921 tatgcttcat aatccagcgt caaaatgcaa gtaaaatccg aaattcctac tgctgttgcg
    13981 tgaaacttcc aatcttacca atttgttata atagaatacc acatcaaggc ctacaccgta
    14041 accataaaca tatttattgg tcagcggatt cccgtaagca taaaatctgt cggcgaccct
    14101 tgcaatatca aatcctccgg tcaaatatac atccaaaggc attttcctgt acgctttgag
    14161 caccatatat tttccccaat ccaactcctt gcccaagacc ttgtatctcg caatgttttt
    14221 caacaggaag taatcctgcc catcgattac attcagatcg taaccacgga cataaatatc
    14281 atcgtaacca attgccttat tgaaataata gggtactttc ccccgatcaa gattgtatcg
    14341 gcccagggtg tgctcctcga gacttagctt tggagtaatt ttaaagaacc ctgataagag
    14401 aagtcctgca tctgacttgt gtatatcatg gcggcttaat ccaatctgac taaaatataa
    14461 gcccagactc catcctttca tggcataagg aactacatcc cgggtatcat agcggaataa
    14521 atagataaat tccggaaagc tcagtttaga tctgttgttg agatagtccg ggttgtaact
    14581 cgtggtcaac gaatcattta tattgaaatg ataatagttt atctccacca aatgttttgc
    14641 atagatagca ggcctataaa acatccgtgc ccctaacttt actcttttga gcaggtcctg
    14701 atccagattg cgctttaata ccagaaaatt gtcaatcgtc ttataggcga cttctctgtt
    14761 ggcggcataa aagcctgtga atcctattcc aagggtattg gaatgattga gataaggata
    14821 atcgtaatca attgaaattt tcttggtaaa tcctgcctga ccatatatcg tgaggcgatc
    14881 accattgccg gtaaggttga tatgacttag gaaaagccca tatgataaac gtttcaaaga
    14941 tcgattgtaa ttgcgccacc atttgttgaa atcattgtcg ataaattcca gcaagggtgc
    15001 aggatagata tataaagcct ctttgcaatg aatgttgaca ccggccgatt gtgcctcgat
    15061 attcaggttg ataagattgc atctcgcctc caccaccaga ttggtattca gaagtcgttt
    15121 ttcattggtt tgaaagatag aagcaaggta ttgcagatcc aatgaatcac ccttcttgaa
    15181 attcaattca cgaataagta tgtattcccg tgttttctcc agtccgctga tcgaaatact
    15241 atctatagca accttttgcc ctgaaaggca ataagaggct acaaagaaaa agaagaagaa
    15301 cgtatatctt cccaaatatt gcttttgatg gagaatcctg aactgcacga gatgaatcaa
    15361 cgcatctttg gcctgcgttg cttgcttccc ccacgctgtg acatggccat acgattctgc
    15421 atatttaatt gtcccgattt gcccagtgct tgcttaaact gcttgacctg gtctttgatc
    15481 attgattcgg tcaccttgag ttcgtctgct ttcttgacca atgtcgctgc ttccctgtag
    15541 ttgcccttga ctgctgcaag attggcctgt tgcaggtaaa tcattgcctt ttcgttgtcg
    15601 gaagtatatt ttgagttttc actctcctta aacattcctt ttgccccctc gtaatccttg
    15661 ccttgcatgg caaaagtacc tttcaacata tagtacgcag agcgaccggg gccgaatagt
    15721 aacttaggaa agaatgtcaa tgcaagcctt ttctttgctc cttccatatc ttgtgcctgc
    15781 atcaactgac tggcagactg gacagttcca accatcaaat aacccaccag aagaataagt
    15841 gcgaccaaat ataaagggaa ggcataccaa aagccataga gcgtggtaag gacaatgccg
    15901 gcaataatag agccgactat caacgcaaat cgtaaaaata tattgatggt aaacatagtc
    15961 agttttttcg caaaagtaaa aaaatccgct tatttcaaaa agacaccggg tatttaagaa
    16021 ataagcggat cgagataaca gaagatcaat attatatctt taatgagctc cgctaagtgg
    16081 cctctgccta ctccagctac ctatatgagc gatgccccaa tggtccgggc tcgcattatc
    16141 aaagtccagt ttataccgct ccggaatctc cttcaggaag gatccggtct catattcagg
    16201 gaaaatctcc cgatacgtca gcatagattg gaatgaaatc cttcggtaaa tgtgcgacct
    16261 ggtgacgttc gacactttat ccagaccgga cgctccaagc aattcgacaa cactttccac
    16321 tgtgcctttg tggaaattag ctacccgggt ctttttatcc tctactacca gtccaaccgt
    16381 caatttggga tcctgagtag ccacacctgt aggacatttg ttggtattgc aaagtaaagc
    16441 ctgaatacat ccaacggcca tcatcatggc acgtgcagaa taacatgcgt ctgcccccaa
    16501 tgctattgcc cgaatgatgt gaaagcctgt tgtaatctta ccggatgcca atatcttgat
    16561 atggtgcctg atatccaggc cgacaagcat attgtgcaca aaagccaaac cgtccagcaa
    16621 tggtgccccg acatagttgg aaaattcttg cggtgcagca cccgtaccgc cctcagcgcc
    16681 atctacagtg atgaaatcag gaaaaatatt aagctccatc attgccttgc aaatgccgat
    16741 aaactcgctc ttatggccaa tacacagctt aaaaccaatg ggtttgccgt ccgccagttc
    16801 ccttagctgc ttgacgaaca taaccaggcc tctcggtgta tcgaatgcct tgtgataagg
    16861 gggagaagca acaagagtat gaggttcgat accacggata gctgcaattt ccggtgtgtt
    16921 ctttgatgcc ggcagtatac ctccgtgacc gggtttggcg ccctgggata tcttgatttc
    16981 aatcatcttg acctggggtg tggcggcttt ctttctgaaa gcttctgggc tgaaattacc
    17041 atcagcagtc cgacagccaa agtaaccggt tccaatctgc cagatgatat ctcctccatg
    17101 tttcaggtgg tgaggggtta gaccaccttc tccggtatta tgcgcaaagc cgccaatttt
    17161 tgcaccggcg ttcatagctt ccacggcatt ggcactcagt gagccataac tcatcgcaga
    17221 aatattatag atgctgcatg aatacggctg cttgcaatcc ttattgccta tgagtaccct
    17281 gggatcatga tcgagcatgg aaaaatcctt gggtgcgatg gaatgacaca accattcata
    17341 accttcagta tatacatcaa gctgagtacc gaaaggcatg gtgtcatttt ctttctttgc
    17401 acgctgatag atggtcgaac ggtcgattct gttgatggga cgtccgtcga tatccgattc
    17461 aataaaatac tggtaaattt ttggtctgag ttcctccatc aggtaacgta accttccaag
    17521 aaccgggaaa atccgcatca ctgcatgttt tttctggaac atatcataat aaccaaggat
    17581 gaccaaagct gcaattattg ccagcaccac ataccagtta ggccccagga accatgccag
    17641 gagaaataca gcaataagca aagcggatga tatgatgaca aatttttgtc tcatagttcg
    17701 tataggattt tatttcaaca taaatgaaat gaatgatcgc agaaagttca ttaattagct
    17761 gcaaattata tcaattaatt tttcacggct aatattttta atttcttcaa tccaccggga
    17821 aggctccatt cgactgtatc tcctttactg aatccaatca atgcaactcc caaaggcgaa
    17881 aatacggata ttctttgttt tgtcatatcc gcttcttcag gaagtgtaag tgtcaaagtc
    17941 caaatttttt gagaatccaa atcctgggca tcaaatttgg agcccaatcg gatgatgtcc
    18001 ggattgatag cctcatcctc tacgatcttg gccctgtcaa gctcaatagc caggttgcca
    18061 acttctttgg tcttgagctg gacaggatag ttagctatta aagtcttaag ggtcttgaag
    18121 tcagttgaat taatggttgg tatcataata tttcaaaatt ttaaaagccc tcctttcccc
    18181 ggctgtttct cacgccgacc aaaaggctga ctaattagtt aaattaaatt taaattttta
    18241 gaaaacgaag cggaacccct ccgttttaca caagaatatt aacggagggc tatagtgata
    18301 aaggccttat tatgggtatt tgctgatacg agtgtatccg tattgcctga agatctgtat
    18361 ttgtgttgag tttttaccat tacggctcaa aattatcaaa taaaacgcaa agatacataa
    18421 taacggttag attgaaaatt agtttcttgt tcaaagttaa ttgtacacga taaatgttta
    18481 atttgttaag gtctaaaaat cactgtatga cataaacttt agattaaatg acctgctaaa
    18541 tccgctaatt ttccctcgga ttggtgaaca atagccctgt atgaaaaata aaaacagaca
    18601 tatccgccac caaaataaaa cccattgtca aatccgaaca tatcaagctg aaaaggcaag
    18661 gttatatcga gtcaaatatt tttaagtaag cacaaatcag gaaagctccc gtccctgaag
    18721 tcacatcgac aaggtgcctg taagtacttg acaaattaga agtatatttc cactattttg
    18781 tctgattggc cttagaaccc agtgcccagg attatgaaac accaacctgt ctctcagggc
    18841 agttgattcc gtgcaatcag gtttcaaaca gctttgtgcg gttcttataa ttaaaatttg
    18901 aacaactctt tatttgatta attgatccta tcccaatgat atcgccgatt ggttgatatt
    18961 tatttataaa atttaaaaaa agaccattat atcccacttg tttagaatat ttctaaattg
    19021 ccaacttcca tagcggttat gccctatctt ttttgaattt attattttgt taatacattt
    19081 gtggcaacaa gtaaagtaca aaacatgaat tggtttaata gcttcattac ttccagggtc
    19141 gggcaaaaat tcgtcatgag tttgacggga ttgttcctga tttcttttct attggtgcat
    19201 ttagtgggca atctacagct attgaaagac gatggtggcc tggcattcaa tatgtatgca
    19261 aattttatgg ttcacaatcc attcattaaa tttatttcaa tcggtctgta catcggaata
    19321 ctgttacatg ctgtccaagg tattgccctg aagattaaga atcaaaaatc acgcggcaat
    19381 aaaggttacg ctgtcaataa atttgtttcc aagtcatggt attcagctaa tatggcttta
    19441 ttgggtaccc tgattctctt ctttatcttg atccacatgg gtgatttctg gttgaagtat
    19501 aagtttggag atgtatatcc tatggttaac atagatgggc aagaattgaa aaatctatat
    19561 tggggtgtaa atcaatcctt tagtcagcta tggattgtcg tagtgtattc agttgctatg
    19621 gtcgtgctgt actttcacct tttacatggt ttctggagtg cttttcagac tttgggcatt
    19681 gacagccaca aatacacgcc cgtcatcaaa tgggtgacca aggttttctc ttttttgatc
    19741 tgtctgggct ttttgatcat accgatatat tgttacctca acagttgata ccgacacggt
    19801 aataaattat taatgaatta gttttaatcc aagatattat gagtttagat gctaagattc
    19861 ctgaaggcga tataagccaa aaatggtcca actataaatc caaggtacat ctggtcaatc
    19921 cggctaataa gcgaaatctc gaagtagtcg ttgtaggatc cggacttgcc ggtgcttccg
    19981 ctgctgccac actggcagag atgggataca aggtcaaggt gttctgcttt caggacagtc
    20041 cacgtcgtgc acactccatc gctgctcaag gaggtatcaa tgccgccaag aattatcaga
    20101 atgacggtga tagcgattac agattatttt atgacactgt caagggaggt gattatcgat
    20161 ccagagaggc caatgtatat cgacttgcag aagtcagcgg taacatcatc gaccagtgtg
    20221 tagctcaggg agtacctttt gctcgtgaat acggaggaat gctgagcaac cgctcttttg
    20281 gaggtgtaca ggtgcaacgt accttctatg ctgccggtca gacaggacag cagttgttgc
    20341 ttggggcata ctcagcgctg gaacgtcaga tcgggatggg taagatcaaa atgtacagcc
    20401 gccacgagat gctcgatgtt gtcgtcatcg acggtaaggc acgagggatc atagcaagag
    20461 acttggtaac aggagaactg gagcgccact tcgggcatgc agtcctactt tgtaccggtg
    20521 gttatggcaa tgtattcttt ctttctacca atgcaatggg atccaacgta accgcggcct
    20581 ggaaagctca taagaaaggt gcctattttg ccaatccttg ttatacccag atccacccaa
    20641 cctgtattcc tgtgagtgga gatcatcagt ccaagctgac attgatgtcc gagtcactgc
    20701 gtaatgatgg acgcatctgg gtaccaaaga agaaagatga tgcacgtccg gccaaccaga
    20761 tacccgagga ggagcgcgac tactatcttg agcgaagata tccggcattc ggcaacctcg
    20821 tgccacggga cgtcgcttca cgtgcagcaa aagaacgttg cgatgaaggc tatggcgttg
    20881 gcacttccaa gatggcagta tatcttgatt tcgcttcagc tatcaagcgc tatggcgcaa
    20941 tcgaagtgac caagcacgga attgccaatc cgactgaaaa cgatattata catcatggta
    21001 aggagattat taaggaaaaa tacggaaatc tgttcgatat gtacaagcag attaccggtg
    21061 aaaaccctta tgaagtaccg atgagaatct atccggcagt acactatacc atgggaggcc
    21121 tttgggtgga ttataacctg atgacaacag tgcccggcat gtacgcttta ggagagtgta
    21181 acttctcaga tcacggtgcc aaccgacttg gagcatctgc attgatgcaa ggccttgcgg
    21241 atggttactt tgtcattcca tataccatag gggactacct ttcaagcgag atccgcacca
    21301 aagcaatctc taccgaccat gaggcatttg ttgctgctga agccgatgtc agggcgcgta
    21361 tcgaaaaatt aatgaatgtt aagggtaaac attccgttga tcatttccac aagaagttgg
    21421 gcaaggtgat gtggaacaaa tgtggtatgg cacgcaatgc acagggactc aaggaggcta
    21481 tagaagaaat acgccaaatc cgggaagagt tctggtcaga tgtaagaatt ccgggagctc
    21541 cggacgaatt caacccggaa ctggaaaagg ctggccgcgt agctgacttc ctcgaattaa
    21601 gtgaactgat gtgtatcgat gcactcaaca ggaacgaatc atgtggtggt catttccgtg
    21661 aagaattcca gacagaggaa ggggaagcat tgagggatga tgagaatttc tcctatgtgt
    21721 cagcctggga attcaagaag gatggtgatt tttcactcca taaggagcca ttgacattcg
    21781 aatatatcaa agtcgctcag agaagttata aatagaattt aatcaaccgg caaatccaat
    21841 ttgctataat ataaatcaag aatgttatga aattgaaact caaagtctgg agacaaaaaa
    21901 acggccaatc tgaaggcgcc ttccagaatt acgatgtgga tgatgtgtct ccggatatgt
    21961 cattcctcga aatgtttgac gttttaaatg aaagactgat aaatgaaggt ggtgatccta
    22021 tcgcttttga ccacgactgc cgtgaaggta tctgcgggat gtgcagcatg tacatcaatg
    22081 gtcagccaca cggacctaac cgaggtgtga ctacctgtca gttgcatatg cgttcgttta
    22141 acgatggcga atccatcacg gtcgagccct ggagggctaa ggcttttccg gtaatcaagg
    22201 acctggttgt ggacagaact gcattcgatc gaattattgc ggccggagga tttatatctt
    22261 ccaatacagg taatgctcaa gatgccaatt ctattccaat cggtaaggaa atcgctgaca
    22321 aggctttcga atctgctgcc tgtataggtt gtggagcttg tgtggcagcc tgccccaata
    22381 gcagtgcctc gttatttacg gctgccaaga ttagccaact taatcgattg ccacagggcc
    22441 agcctgagcg atacatccga actcgcgata tggtacatca gatggataag gaagggtttg
    22501 gagcatgttc caatacaggt gcttgtgagg ccgaatgtcc gaaagagatt tccatttccc
    22561 acatcgcaaa gatgaatcgc gactatatca aggcatcatt gttcaaggat taaacagatc
    22621 tttggtatat tatcaaaata gataacaggg ctgtcttttg gcggccctgt taattttcca
    22681 gatccaggaa agtcgcagta tggctatcct ttaccgcctt caaaccctca ggaggtccct
    22741 ggtacagaat cttcccccca ttccttccgc cttcaggccc gatgtcgatg atgtagtcac
    22801 tacttttgat gacctccata ttatgttcaa ctataatgat agagttaccc cgatcaatca
    22861 gcttattgaa ggctatcagc aattttctca catcttcaaa gtggagtcct gtggtaggct
    22921 cgtcaaatat aaagagggtg tgaggcacct tgttgtcctt ggtcaggaag cttgccagct
    22981 tgacccgctg agcttctcca cccgaaaggc tcgatgacgc ctgtcccagt ttgacatatc
    23041 ctaagcctac atcaagcaat ggttgaatcc gattaataat ttccggttga tcagcaaaaa
    23101 aatcaacggc ctctctgata tccatttcga ggacgtcata gatatttttg tccttatatt
    23161 tgatatccag tacctcattc ttgaatctcc ttcctttaca ttcatcgcag accagactta
    23221 catcggcaag gaattgcata tcgactatgg ttactccctc tcctttacag atttcacatc
    23281 gtccaccttc cacattgaaa gagaagtgct taggcttgaa tcctctcagt tttgataatg
    23341 gctgtgctgt gaacaagtca cggatgtaat cgtaagcttt tacgtaggtg attggattgg
    23401 agcgggacga cttcccgatt ggttgctggt ccaccatttc caccacattg atggtatgaa
    23461 aatctccata aagatcgcgc acaatattgt tggaggtatg tgcactcctg gtttgaatgg
    23521 cgttgacaag tgttggatag agtgcacccc tgacaagcga acttttacca ctaccactta
    23581 caccggtgac gcaaacaatg gaatgcaagg gaatggagac atttactcca tccagattgt
    23641 gaagatagac accatccaac ctgataatat tcctgccgac acgcctttct ttgggaactt
    23701 cgattttctt ccggccaagc aaataatcga tggtcaggct gttgttaacg ggaatttgtg
    23761 caatatcctt ataattaccg gtaaacacga cctctccacc cagaacccct gcttcagggc
    23821 ccatatcgac cagataatcg gcactcctga tgacttcttc ctcatgttcc actacaatga
    23881 ctgtatttcc cagatcacga agcttcttca aggcagttac aagattgcgg gtatctcggg
    23941 gatgaaggcc tacagaaggt tcgtccaata gatacaatga tccggtgaga ttacttccca
    24001 acagcctcgt gaggtggatg cgctgcgtct caccgccact taatgtagat gccggccttt
    24061 gcaatgtgag ataaccaagt ccaatggcaa ccatggtcga cagtcgttga ttgatttctg
    24121 aaagtattcg ttccgcaatg gttacatcgc gttcttctaa tcttatttcc cgaaaaaatt
    24181 catccaactg ttcaatatcc atttctgcaa gctcagcaat atgtcgccca ttgactttaa
    24241 cataaagtgc ctccttacgc aatcttttcc ctctgcactc cggacaaata gttctccccc
    24301 ggtatctggc caaggcgata cgatcctgaa ttttatagct cgtgttttgt aactttttga
    24361 aataatcgtc aatgcccaaa aatccatcac cccctctcca gataatctcc aattctctgg
    24421 aactcaattt gtggtatggt gtatggatgg acaggcccag tcgctccgca ttctggatca
    24481 tcgacttgta aaagttttta cctttatcgc ctgaccatgg agagattgcg tggtcataca
    24541 gactaagatg tttattgggt atgaccttgg attcatcgat acctatggcc attccataac
    24601 cttcacatac atgacaggcg cctactgagc tgttgaaatt gaacaaggcc actgaaggct
    24661 cttcataacc gataccattc tcttcaaatc tcgtactaaa atgatgcatc tcaccttctt
    24721 ctgtgagcag gacacagctg ccattactct ctgcaattgc tgtttctata gaatctgcta
    24781 tacgcttgaa attttccaca tcctcctttc gcactgcgat tctgtccacc acaacataag
    24841 cgacaatttt attattttca ctgtccttat ttattccttg aggcaggttt gacacaagtt
    24901 catctattct ttggacttct ccatcaagcc acaccctgtt gaatcctttt tgctccagct
    24961 tttcgagaac agtcctcagg ggttcttccg cttcgttgta ggaaaatgga ctcaaaatag
    25021 aaaatcgagt gccttcttcc ttgttgttga gaaaattcac gatgtcactg acgctatctt
    25081 ttttaattac cttgccagtg ataggtgaaa ttgttttccc aattcttgcg tacaaaagcc
    25141 tcagatagtc gaagatctcg gtcagggtac ctaccgtact ccttgcattg gcactgctta
    25201 ccttctgatg gatggctatg gcgggagtga tgcctttgat atagtccacc tcaggctttt
    25261 tgattcgtgt cataaattga cgggcatagc tggataggct ctctacataa cgccgctgtc
    25321 cttccgcata gagcgtatcc atagtgatgg atgatttacc gctacctgac acaccggtca
    25381 caacgatgag tttatgcttg ggtatggtca agtctatatt cttcaggttg ttggtccgtg
    25441 cacccttaat tacgatattc aacttatcgt aaagaatcac cggctcgcct tcaggtgaaa
    25501 tttgtttttt tgtccttggc atattttggt tccctatatg gatttgaaag aataaaagtt
    25561 gtaaaagtag tgaattttga catcacgtat caaactcaaa taaagagcat catggttagt
    25621 cataacacaa gacatgtaaa aaaagttaat accagctaca cagattaaaa tttcaaacca
    25681 tgatgacaaa tggtttcagt gtaagagatt aagtgttgtg atgtataaga ttaatgcttt
    25741 gaagtcaggg tcgaaaaaat gaagtaggta gaaaaataat aactgaatat tatgatggca
    25801 ccgatgtttc atcatgctga gaaaattaaa atacgcgatt tggtttcgct tttattcata
    25861 aaattgctgg aaaggccaat ggtaattgaa aaggcgacta ttaatcatct acctacttca
    25921 gaggtgggca tcacccccat cccccaaaat cgaagtaggt aggaaagtaa taattcaaaa
    25981 accgccatac acaaggattt ggaggcaaat gaaaaattta atttccgagt agatcatctg
    26041 aattttggca aagccgcccc gaaaggcagt gcaatcaatc attgcgacta ttaatcatct
    26101 acctacttca gaggtgggca tcacccccat cccccaaaat cgaagtaggt aggaaagtaa
    26161 taattcaaaa accgctatac acaaggattt ggaggcaaat gaaaaattta attcccgagc
    26221 aaatcatctg aattttggca aagccaacct gaaaggcaat accgtcaagc atggtggcta
    26281 ttaatcatct acctacttca gaggtgggca tcaccctcat tcccccaaaa atcgaagtag
    26341 gtaggaaagt aataattcaa aaaccgccat acacaag
//
LOCUS       DAOQMC010000005        22000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_817_length_22000_cov_5.024926, whole genome shotgun sequence.
ACCESSION   DAOQMC010000005 DAOQMC010000000
VERSION     DAOQMC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22000
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_817_length_22000_cov_5.024926"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            745..1143
                     /locus_tag="PKV31_00490"
     CDS             745..1143
                     /locus_tag="PKV31_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNB91352.1"
                     /translation="MNPGQYIILYDGDCGFCNQSVMFIIRNDRQKNFLFASLQSEIAG
                     DLLKPFNIRPSLNSMILLKEGQYYEKSGAALEIVQHLSGVWPLLSLLKIIPRPIRDGV
                     YCWIARHRHRFLSDTACTLPIPDVESRFLK"
     gene            complement(1236..2594)
                     /locus_tag="PKV31_00495"
     CDS             complement(1236..2594)
                     /locus_tag="PKV31_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB91353.1"
                     /translation="MKIRVKLTLQYFVVVATILILSMFFVYRRFQDITYNEYYNNLKT
                     KAHMTAEMVLHKGDLDKLEDITPHKDSENLPMSDNVIIFNNNYEKVFTFNRKSAFVLP
                     FDIRPISKREFSYHTGDMYYFGLPYTSMSGRKYYVVASSVFNSKELMDLKRIISLAII
                     LGLVVVGVSGFFYTRQALNPLFSIVHQMDQLDMSKLDSRLKRSYTKDEIEHLIQSINN
                     LLSRIQSSVTFQKMFISNVSHELKNPISIVLSQVDVLLNQKDRTVDEYRATLTSLRDD
                     VQDIAYITENLLQMAKIKSEGFHLDIQNFQLDEAILECQSKLLRSHPEYSVSFQIEGQ
                     PDSQEKLTVEGNEALLKLAFINIMDNCCKYSDDKHVDIRINSTDRGLELSFRDKGAGI
                     AKEDMEMIFQPFYRNINTKHVKGYGIGLSLIDAVLQVHKLKLKIESTPKVGSLFAIPF
                     TR"
     gene            complement(2591..3283)
                     /locus_tag="PKV31_00500"
     CDS             complement(2591..3283)
                     /locus_tag="PKV31_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB91354.1"
                     /translation="MKILIVEDEKKLNSSLKKGLEEQGFAVDVAYNGIEGKKLAFANQ
                     YKVIVSDIVMPQSTGLQMLKALRQNNIHTPVLMLTALNMVEEKLEVYAAGADDYLTKP
                     FDFRELVARIRALIKRLPKVAAPSQSEITYADITINPATKKVRRQDINIFLTPKEFEL
                     LHYFMTHPNTTITKQELLSNIWDFDFETSTNVLEVYINFLRNKIDKDFSEKLIHTLPR
                     IGYIFKIQHEEQ"
     gene            3674..4663
                     /locus_tag="PKV31_00505"
     CDS             3674..4663
                     /locus_tag="PKV31_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91355.1"
                     /translation="MSVRLSVSKLIALWAFSESALGGLLHATNMPFSGLLLGGIAVTV
                     ICCIGFTAVRPGAAILYGTLVAVSVKFALSPHSPVTSYIAVGFQGIVGALMFSFQRRS
                     LPVYIFYGGIALAESALQKVITLTVIYGNALWDSLNVAAQQIAKSLHMDSSLSYAVIL
                     LCIYSMVYFVWGCLIGYWAHKIPHEIKNIDKSEIISIDTSGKLFNKKKRKIRYLVWMP
                     MVLILSVSVILMRQSGLSVLQLILRPFIVVMVWWFVINPFLKAMLARFLNGRSNNMAR
                     IIDSIRQDIPRLRKLSFQTADYVTRHYRGVSRVRRFITILIILASEEKSNLTG"
     gene            4635..5102
                     /locus_tag="PKV31_00510"
     CDS             4635..5102
                     /locus_tag="PKV31_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNB91356.1"
                     /translation="MKKKVILLDNLWVITGEVNSGKTTSLLQWTRGRKVAGFLTPDID
                     GQRRLMDLSTSEILPFEANDDDEDVQKVGRFSFFQSAFDYGNSLIIHSLQADHPYFII
                     DEFGKLEINGQGFAYGIDTLMGGIRTSQSPTIYIIVIRESLLDGFLERYGLSD"
     gene            complement(5212..5370)
                     /locus_tag="PKV31_00515"
     CDS             complement(5212..5370)
                     /locus_tag="PKV31_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91357.1"
                     /translation="MHQNIPEAIIGDRWQEVNTRHIFLNCRVYNILYSNIIPGGRHGY
                     ILTLWSFG"
     gene            complement(5536..6033)
                     /locus_tag="PKV31_00520"
     CDS             complement(5536..6033)
                     /locus_tag="PKV31_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91358.1"
                     /translation="MKLLIKQGLTFSALLISFLLTLSFIGCNRSQKEGPSDPVSPQDT
                     GQVIQFSEVDRATARTAIHTYRGPTQQNAGFSFVDVTRDEFQALQQMFNDTTSKYDKV
                     RIYFGIDTFRLDASPTKSFFTGFVGKAEKGARGDSTSFIIVQRIIKGSAPCPKTCDFT
                     KSIFR"
     gene            6172..7965
                     /locus_tag="PKV31_00525"
     CDS             6172..7965
                     /locus_tag="PKV31_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91359.1"
                     /translation="MSTDPFLAICRTFLFLTIIMLGMLGCKRGSEERHNDSKIVTNDF
                     GQGIPGILYRAKVQLDSFGFVNKADSLLELAINEAVKAGKNDQIAMAYTFALKYSSSK
                     PVNEAQALEFYNTGKYHAGLSGSISLLNELNFAMAHYYTKRGNTGQSEKYLNQISFFG
                     GSNFELYLKKEMAEAANFGVRDKPFEQLKSLLDAMYKAYELGHDSIYQACLLNISDFY
                     YYEQQYNKALDYIDQLQNTIAAKGIQTDSNRIYFLMANKLPIFNKQFDQNATIRLAES
                     ISHYARKHGYQLLEEIAQGQVRTALISSGDFKRVEDYYRAYPVYLSRLEHEKPSIYYR
                     MLSYIEMAKGKKDSAVAYFDKAGEFLDGENEYYVANYHFRKGELMEKLGQRSESISSY
                     SKALKMLNNSHDLTLQNTLIDKLIPYYKEINDPRSIVGLLEKKDSLNMKLIQINKEQN
                     IRQLELSNILSVYELKHQKAAEELNRKQVIQLQIVALFILLVFMSLLVMANLKLPGWW
                     LRSMGYISIIILFEFIILMVDRRLHDMLHGSVIKLLVIKVILISWILPLHHWAEKKIV
                     AFLLQKDRFRGLKDFLSRLFQSFGKKEVEES"
     gene            complement(8026..8742)
                     /locus_tag="PKV31_00530"
     CDS             complement(8026..8742)
                     /locus_tag="PKV31_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91360.1"
                     /translation="MDFTSIYDPSVFDRFLTIGSGSYREVIHFYEDHRNKIENLDARH
                     RFEIYVCYIEALFEMGKFKEVLFLVDDAIETAIELGFSRLFGVDIYFNLLMKKAVALN
                     EAGRVEDSLSIIRQLLSMNPDEPNVRYFFKEIMMNRSRKLVLPTRVFFIAGVITTTIL
                     MAIEALVIMPFYSDLIDPVSGVRNVLFLFSVLIFISGEVSRFLLIEWHCLLLIRSIKK
                     HRPTNTIKGSNTRRVIRHKK"
     gene            complement(8744..9517)
                     /locus_tag="PKV31_00535"
     CDS             complement(8744..9517)
                     /locus_tag="PKV31_00535"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNB91361.1"
                     /translation="MNVETYNIEVSVTLNGQKYTADLHHPIDISMILHSGYPQVNCFR
                     APYFYASPHREGDLVKSIAQGGHANFYNVTIHPHGNGTHTQCVGHIVRDQYYINEYYK
                     PDFHVAQLVSIFPQLSDNGDKVIHAEQLSQLWEAVGEEALIIRTLPNGPEKRKTEYTD
                     TNPPYFSRDAIDLIVSRGIKHLLTDLPSLDREENQGKLEAYRAFWNYPDIDLRAGATV
                     AELIFAENFVEDGLYLLNLHLLNIQLDVSPSRPFLYRLR"
     gene            9750..10373
                     /gene="tmk"
                     /locus_tag="PKV31_00540"
     CDS             9750..10373
                     /gene="tmk"
                     /locus_tag="PKV31_00540"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNB91362.1"
                     /translation="MAKPVFIVIEGIDGSGKSTQIELLQHRMSEEGKQVAITGEPTDG
                     FIGKIIRDIMRGKEETDPAVVAALYVADRMDHITHPDVGMLALLEKGYNIIASRYYFS
                     SYAFQGEYVSLEWLIHANSICKSRLKADLTVYINIDPAICFERIKSSRPSLDIYENLE
                     KMKRTHREYMKAFEVAGMDENIVMVDGTLPIDLLHEAIWSKVKEVAF"
     gene            complement(10484..11368)
                     /locus_tag="PKV31_00545"
     CDS             complement(10484..11368)
                     /locus_tag="PKV31_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNB91363.1"
                     /translation="MSLFRILPDRIDVVQSQNRPDDKNSWQYKLYVIIFQANTKAGKM
                     FDIILSLMILINIFIILAESIESLSAKYSFYFRQADRFFLFVFTLEYLLRLSCVKVKK
                     RFVKSTFGMLDLIAILPSYFEYFLPQSHMLMIIRSFRLLRVFRIFGMVKFLDESRELV
                     FALIRSFRKIAIFLFFILILAFLLGSLMYLAEYQHNPNFHSIPQSIYWAIVTLTTVGY
                     GDITPITPIGKILASFIMILGYSIIAVPTGIIGGNIIKEMKKTNINLVCENCGTKEHS
                     NDAVYCRQCGERLHQPVI"
     gene            12113..14950
                     /locus_tag="PKV31_00550"
     CDS             12113..14950
                     /locus_tag="PKV31_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91364.1"
                     /translation="MRIFILSIICCISFVFALPAQQGVYNMVDFYRELSVGHPVLPFP
                     ENVKFESGLNKYIGEPMALLGQRAPGSRSGQQNEMSAVTMTEIEHLFSGRIQRGVVHS
                     ANQPPVADAGPDTTIVLPVDSVLLNGSGTDPDGTIVSFEWTPDPNNPSAATFDDASLP
                     NPMVSNLIEGIYIFTLTVTDDQGEIGSDQVTITVQAANQPPVVNAGDDLTIHLPESSV
                     QLTGTAIDPDGSVVSFSWSQDPSNPAPAAFSNFSIPNPVVSGLIEGVYSFTLTAVDNE
                     GATGQDNVLVNVLPPINLPPDAHVGFDLRVILPTDSTILHGSGTDPEGAPITFLWKQI
                     GDLPSIATISDSASSDPVVSDLILGIYTFRLIVTDDKGLSDSVEMNVIVDIVGNIKPI
                     ANAGEDATILVPQTSYQLDGSGSDSDGFIIGYNWEQIGTLPNTAIISNPGVPNPIVSG
                     LIKGAYTFRLTVTDNEGGKGMNDMVLIVFDTFNIAPIANAGPDATIALPQDTYQLSGS
                     GTDADGIIISYHWQQVGNTPSQAVFSNTDIPNPVVSGLIYGHYTFRLTVKDDYGLTAS
                     DDIVLFVRDSFNHIPVANAGENIRLTLPDNSVQVHGTGYDDDGIIVGYEWQQVGNKPS
                     KAVISNPDIADPIIGGFLYGTYTFVFTVRDNEFATGSDTLLIVVDTVPLIAPIADAGA
                     DISVNLPQDSVQLHGSGSDEDGTIVGYTWYQLGTQPSPAAFSDTMIADPIVKNLVEGT
                     YHFVLKVTDNDNLMDTDTVRVVVSKAKNIPPVANAGEDITIKLPIDATTLHGSGSDDD
                     GTVTDFLWEQVGTTPDIATIIGNNSPEPTVSNLFKGTYTFRLIVTDNEGAKDTDEVKV
                     FVLDKLSTHTGDVSSAIYPNPAKGGIFHINPGAKLKPGTSARIFDIDGREMMRFVVGR
                     EDSSFQCNQCVSGVFVIRFDNGMSAALVVMP"
     gene            15544..16677
                     /locus_tag="PKV31_00555"
     CDS             15544..16677
                     /locus_tag="PKV31_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91365.1"
                     /translation="MLTIQDIQGSDNVKKEITTLISSGQFPHTTLFIGPEGSEKLNLA
                     YATARLLMCENNKNGVICGQCNSCRKSANFTHPDIFFSFPFVKEGSGDFQSDDFLQAW
                     KEAFRENPYMNVYEWSLKQDAGNKQPNITAFECIQIQRKLQKKPYESDRKVLLLWMPE
                     YLGKESNRLLKILEEPPGDTFIILVAEDQERLLATILSRCQLFKVEPFDLQGVQRILE
                     NHFGKSKEEATMAAYLSRGSMVEALSLFSEGNLEKNGEFVTWLRLCYSFNHVEIIRWI
                     EHFATYTREVQKYIFKFGIHFVNEMLKIKYNYGVDNISLPKEMADVAARLCPLLSFES
                     IEELVNTLDREIFLTERNCNTKMQLIYTSGYIHRLFNGKRFAA"
     gene            16701..18155
                     /gene="ricT"
                     /locus_tag="PKV31_00560"
     CDS             16701..18155
                     /gene="ricT"
                     /locus_tag="PKV31_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNB91366.1"
                     /translation="MGCNSCSTEDGKPKGCRSNGVCGTSGCNKLNTFDWLTTMELEDP
                     TEVNLVEVSFRKGAHKSFFRLQDAYQAEIGDDVVVDADGGYDIGRISLKGALVPIQMK
                     KKSVDPNSALRFVIRTASQNDMAKLEQAISMNRNTLIRSRAIAATLGLDMKIGDVDYR
                     ADLKKATFYYTADGRVDFRELIRHFAKEFRVKIEMRQIGSRQESSRIGGMGSCGRELC
                     CSTWLTDFKSVTTNAARYQNLAINQAKLSGQCGRLKCCLNYELDVYMEALEDFPTNAD
                     VLRYQGGQARLIKTDIFKKLMFYIKKDEKGREAFITLRVDEVLRIQEDIAHGKLPEDI
                     TSTKVEAFEDDAVIKSEDLTGHIELPEEKRRKKKKKKNPENKPMRHEGQKQEDKKETG
                     NEGKDNNQRRQDNRRNEHRDNRNKAGRPQQGRSDQPDKKPEPRDKGVDRPESIDRNQE
                     KSNNQNKRNKKHRNQQRRSGNRPNNGNAAPPADA"
     gene            18184..19584
                     /gene="lpdA"
                     /locus_tag="PKV31_00565"
     CDS             18184..19584
                     /gene="lpdA"
                     /locus_tag="PKV31_00565"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB91367.1"
                     /translation="MTYDVVVIGSGPGGYICAIRCAQLGLKTAIIEKYKSLGGTCLNV
                     GCIPSKALLDSSEHFFNAAHQMADHGINIGKVEMDAKKMFERKKNVVDTVSKGVSYLM
                     SKNKIDVHYGVGSFIDSSNIKVNGEEGESTLTSKYFVIATGSKPITPAAFNYDKKRII
                     TSTEALVLGEIPKSMVIIGAGVIGLELGSVYARLGAKIDVVEFTDKIIGTMDVDCGKE
                     LQKVLKKEGFTFHLNHAVTTVKSTKTKVTVEFKQNGTDEVKKIEADYCLIAIGRRPYT
                     DGLNAEAAGVIVDAKGRVEVGDHMRTNVPNIFAIGDVVRGAMLAHKAEEEGVFVAETI
                     AGMRPHINYNLIPGVVYTWPEVASVGETEGQLKERGVDYKVGKFPFKALGRAKASGDE
                     AGFVKVLADKNTDEILGVHMIGPRAADMIAEAVVAMEYRASAEDIGIISHAHPTYTEA
                     FKEAALDATGKRPLNI"
     gene            complement(19981..20856)
                     /locus_tag="PKV31_00570"
     CDS             complement(19981..20856)
                     /locus_tag="PKV31_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HNB91368.1"
                     /translation="MASGIFAVLDDIAALMDDVALASKVATQKTAGILGDDLAVNAEK
                     ATGFVSSREIPVLLAITKGSFINKLIIVPIALAFNFFFPVVIKYILIAGGCYLAYEGA
                     EKIIEYFFHRNKGHHIHQETDHNIEEDSEKKKIKAAITTDFILSIEIVIIALGTVLEK
                     DLVIQVLTVSFVAILATIGVYGLVAMIVRMDDAGYFLIRHSKSNSWRSKIGNLLVKAL
                     PYVIKMLSVVGTIALVAVSGGIFVHYIDYFHHLWPKFPILLKELTFGITIGMVLVLIL
                     SSAKWIGKKLKKTPA"
     gene            complement(21020..21493)
                     /locus_tag="PKV31_00575"
     CDS             complement(21020..21493)
                     /locus_tag="PKV31_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91369.1"
                     /translation="MGVKTTRIILLEMLWVAGITILALIVMIPIYAHAREFPFYLDNF
                     IFVFALFTYARFMFFVSTSILTLHVGVKLFFLATAFPFSFLLLDQFNNFRTYIDNHGT
                     QPFFGHLPDKLQIPIDIYLKNEMVLFGVGAIVSSILFPFFLVYSIWVFRNKGRHI"
     gene            complement(21710..>22000)
                     /locus_tag="PKV31_00580"
     CDS             complement(21710..>22000)
                     /locus_tag="PKV31_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91370.1"
                     /translation="NALTTYKILNIVDGKTIIRFHPVTGRTHQLRVHAAHHLGLNAPI
                     EGDDLYGTRKDRLYLHAETITFKHPTTREIMTFQSKAPFCQEHLQAITAQED"
BASE COUNT         6411 a         4448 c         4834 g         6307 t
ORIGIN      
        1 aatcataatt gaccagttct gttggttgtg tatagcctga tgtccgggcc agaatcccca
       61 aaagtaaaag gcaccaaact ctgatctctg tcatactcgt tgtatttgat tattcaaaag
      121 taatacgaat gcgatattta ttatagttaa ttcacatgaa acaactccgg ttgccgataa
      181 ataaacgtaa aattcaggaa aaaccagtct tttgccaatt tattggcgaa ttgataacaa
      241 attatctgcc tgttgagaat ctaatctttt atatttcggt acctgcccac cagttttgtc
      301 atttcccgga attagatctt ttgtcagaat cccgacttag gccgcttgca gatctgtttt
      361 tataatattt tcaaaagact tattgttctt gcacatgggc aaagcaggta ttgattggag
      421 gtcaaggaag gcttgaatgc cggtcgccta tagacaacac ctccaccgga tattttgaaa
      481 gatcctgtaa tcaatcagga atttcagttt ctccaagttc atcctgatct ttctcaagcc
      541 taccatatca aactcgtgcc ggcaacttcc gccaattgcc tgagagcaag caaactcaat
      601 tttgtacgaa aattgttaaa agccttcctc ttgtgcataa attattagaa atcagggacc
      661 cgataggcta tatccaattt tatataaagc gaaccaactc tttttattaa aaaccgttta
      721 tcaaaagtca atgatactaa ggaattgaat cccggacaat atattatcct gtatgatggc
      781 gattgtggct tttgcaatca gtcggtcatg tttatcatcc gaaacgatag gcaaaagaat
      841 tttttgttcg cctccctgca gtctgaaatt gccggtgacc tactcaaacc attcaacatc
      901 agaccatcgc tcaattctat gatattattg aaagagggtc aatattatga aaaaagtggc
      961 gcagcactgg aaatagtaca acatctgtcg ggtgtatggc cgttgctctc attactgaag
     1021 atcattccgc gtccaatcag agatggcgtt tattgctgga ttgcaaggca tagacaccga
     1081 tttttatccg acactgcatg caccctccct atacctgatg tcgagtcgag attcctgaag
     1141 tagcgttaca tttacactag gagggagtca catggaaaca gagtcgccgg tagggatgtt
     1201 gttctccggg actaaatcgg cagactgata gacatttaac gggtaaaagg gatcgcaaaa
     1261 agagatccta ctttcggtgt gctttcaatt tttagtttta acttatgtac ctgtaacact
     1321 gcatcgatca gcgaaagtcc gatgccataa cccttgacat gcttagtatt gatgtttctg
     1381 tagaaaggct ggaagatcat ctccatatcc tctttggcaa tgccggcacc cttatcgcgg
     1441 aaactgagct ccaaaccccg gtccgtagaa tttatcctga tatcgacgtg tttgtcatcg
     1501 ctgtatttac agcaattatc cataatattg atgaaggcca gtttcaacag cgcctcattt
     1561 ccctctacgg taagtttttc ttgagagtcc ggttggccct ctatttggaa gctcactgaa
     1621 tattccggat gagaacgaag taacttggac tggcattcca ggatggcctc atccaactga
     1681 aaattttgaa tatcgagatg aaaaccttct gatttgattt tggccatctg caagagattc
     1741 tccgtaatgt aggcaatatc ctgaacatcg tcacgtaaag atgtcagggt agcacgatac
     1801 tcatccaccg tgcgatcctt ctgattgagc aatacatcga cttgggagag gacgatggaa
     1861 attgggtttt ttaactcatg agaaacatta ctgataaaca ttttttggaa ggtcaccgaa
     1921 ctttgtattc ttgaaagcag gttattgatg gactgtatga gatgttctat ctcgtccttg
     1981 gtgtaagagc gcttcagacg gctgtcgagc ttggacatgt caagttggtc catttggtgg
     2041 acgatggaaa aaagtggatt cagtgcctgg cgtgtgtaga agaatccgga tacaccgaca
     2101 acaaccaaac ctaatataat agccagtgag atgattcgtt tcaggtccat caactcctta
     2161 gagttgaata ctgatgaggc gactacataa tattttctac cgctcataga tgtgtaaggc
     2221 agcccaaaat agtacatgtc accggtatgg taggagaatt cccgcttcga tatcggccgg
     2281 atgtcaaatg gaaggacaaa agcagacttc ctgttgaaag taaatacctt ttcataattg
     2341 ttgttgaaaa tgatgacatt gtcactcatg ggtaggtttt cgctatcctt gtggggagtg
     2401 atatcttcca gcttatcgag gtctcctttg tgcagcacca tctcggccgt catgtgggct
     2461 ttggtcttta ggttgttata gtattcattg taggtgatgt cctgaaatcg tcggtacaca
     2521 aaaaacatgg acaaaatcag gatggttgcc accaccacga agtactgaag cgtgagcttg
     2581 actctgattt tcattgctct tcatgttgga ttttgaaaat ataaccaata cgtggaaggg
     2641 tgtgaatgag tttttcgctg aaatccttat cgattttgtt ccgcaggaaa ttgatatata
     2701 cttccagaac gttggtactg gtctcaaaat caaagtccca gatgttggaa agcaattcct
     2761 gcttggtgat cgtcgtgttc gggtgtgtca tgaagtagtg taacaattca aattcttttg
     2821 gtgtcaggaa tatattgatg tcctggcgac gtactttctt ggtggccgga ttgatggtga
     2881 tatcggcata ggtgatttct gattgcgaag gggcggccac tttaggcaga cgctttatta
     2941 gggcgcgtat ccttgccacc aattccctaa agtcgaaagg ctttgtcaaa taatcatcag
     3001 cacctgccgc ataaacctct aatttttctt cgaccatatt gagagctgtc agcatgagca
     3061 caggcgtatg aatattgttc tgcctcaatg cttttaacat ttgaagaccc gtgctttgag
     3121 gcattacaat gtctgacact atgaccttgt attgattggc aaaggcgagt ttctttcctt
     3181 ctattccatt gtaggccaca tctacggcaa aaccctgctc ttctagtcct tttttaaggg
     3241 aagagttcag ctttttctcg tcctctacga taagaatctt catgcaatgc tttgcccggg
     3301 ctaattgttg aaaatatatc ggcaaagata ataaaaaaat gatttgctac tcattatgtt
     3361 aaaaatattt aatgtgtaaa tgaatatgtt tcagtcaaaa caataattta cccggggtta
     3421 tatatcttca tttattttaa caaagacaaa agcttttcca ttagtattta aggacgagtt
     3481 gttactttca aattatatgg gaattttttg gaatttattc aaaatgattt tcctgccctt
     3541 gtggtgaatt tattttggtt tttttaaaac caagaactcc gggtatcaat aaattttttt
     3601 catctttcga agattagtta ctatcttcat atctctattt ataaatttac taccttaaat
     3661 gatagctgac tctatgtctg tacggcttag tgtttcgaag ttaattgcgt tgtgggcctt
     3721 ttcagaaagc gcgttgggtg gtctcttaca cgccaccaac atgccgtttt ccggtttgct
     3781 gttgggcgga attgcagtca cagtgatatg ctgcatcgga tttaccgcgg tgcgtcccgg
     3841 tgccgccata ctttacggca ccttggttgc agttagcgta aaatttgccc ttagtccaca
     3901 cagtcctgtt acttcctata tcgcagtcgg ttttcaggga attgttgggg cattgatgtt
     3961 ttcattccag cgcagatcac ttccggtata tatattttac ggaggtattg ccttggccga
     4021 atcagccctg caaaaagtca tcacccttac ggttatctat ggtaatgcct tgtgggattc
     4081 tttgaatgta gcggcacaac agattgccaa atctttgcat atggacagta gcctgagcta
     4141 tgccgtcatt ttactttgca tatattcaat ggtttatttt gtttggggtt gcctgattgg
     4201 ttattgggca cataaaattc cgcatgaaat taaaaacatt gacaaatccg agatcattag
     4261 catcgacact tcaggcaagt tgtttaataa gaagaaaagg aagatacgtt acctggtgtg
     4321 gatgcctatg gtattgattt tgtctgtttc ggtaattctt atgcggcaat caggcttaag
     4381 tgttttgcag cttatcttac gtccctttat tgtagtcatg gtatggtggt ttgtcatcaa
     4441 tcccttcctc aaggcgatgt tggcgcgctt tttaaatgga cgcagtaaca atatggcgcg
     4501 catcatcgac agcatcaggc aggatattcc caggttgaga aaactttcgt tccagactgc
     4561 cgattatgtg acccgccatt accgaggggt cagcagagtg cgccgtttca taaccatcct
     4621 aatcatcctg gctagtgaag aaaaaagtaa tcttactgga taatctctgg gtcattaccg
     4681 gtgaggtgaa tagtgggaag acgacttccc tgctccaatg gaccaggggc agaaaagtag
     4741 ccggcttcct gacccctgat atcgatggac aacgtaggct gatggatctt tctacatcag
     4801 agattcttcc atttgaggct aatgatgatg acgaagacgt ccaaaaagtt ggcaggtttt
     4861 cgtttttcca atcggcattt gactatggca atagcctgat catccattca ctacaggcag
     4921 atcatccata tttcatcatc gatgaattcg gcaaacttga aatcaacggc cagggctttg
     4981 catacggcat cgacacgttg atggggggca tccggacttc acaatctcca actatctaca
     5041 tcatcgtcat ccgggagagc ctgttggatg ggtttttgga gcgatatggc ttatcggatt
     5101 gagtgatggg atatgactcc tttggcccaa tgactatatt gggaatatcg gtgtatacaa
     5161 tgttcggttt gtgttatgca cgttgatagt atgaccttct tttatgccat cttatccgaa
     5221 tgaccataaa gtcaggatat aaccgtgtct tccccccgga ataatatttg aataaagaat
     5281 attgtacacc ctacaattca aaaagatatg ccgggtattg acttcctgcc acctgtcgcc
     5341 aataatggct tcgggtatgt tttgatgcat acggaaaggt taatcaacgg atgctgcagt
     5401 ccgttcattt agtaatttcc ggctggtctg atatactatc ttgagattct ggtcggtatc
     5461 cagtttctta ttgagctgaa gaagtcgtaa tcagaccaat cccaaactat cctattcttg
     5521 gaatcaccct tgatcttatc taaaaatgct tttggtaaaa tcacaggtct ttgggcatgg
     5581 tgcactacct tttattatgc gttgtacaat tataaaagaa gtgctgtctc cccttgctcc
     5641 tttttctgcc ttaccgacaa atcctgtaaa gaatgattta gttgggctcg catcaagtct
     5701 aaatgtatca atgccgaaat atattctgac tttatcatat ttgctggttg tatcgttgaa
     5761 catttgttgc aatgcctgaa actcatcccg ggtcacatct acgaaactaa aaccggcatt
     5821 ttgttgcgta ggtcctcgat aagtatgaat agctgttctt gcagttgctc tatccacttc
     5881 cgagaattga attacttgtc ctgtatcctg aggagagacc ggatccgatg gtccttcttt
     5941 ttgagatctg ttacacccaa tgaatgaaag ggttaaaaga aatgaaatta aaagggcaga
     6001 aaaggttaat ccttgtttaa ttaataattt catggcttgc gtatttaatt ttatgtaagt
     6061 ataagcaggc aaatttaaca aatgatttat atttgtttat aaaaatattt atacagtaaa
     6121 gcggctgtgt cacaaaagga aattattttg aagtttaatt gttgttatag aatgtctaca
     6181 gacccgtttc ttgctatttg ccggacattt cttttcctta caatcattat gttgggcatg
     6241 ttggggtgca agcgtggtag cgaagaaagg cataatgaca gtaagatagt gaccaatgac
     6301 ttcggtcaag gcattccggg gattctttac agggcaaagg tacagttgga ttcctttggt
     6361 tttgtcaata aagcggattc tttgctggaa ctcgccatca atgaagcggt caaggcagga
     6421 aaaaatgatc aaatagccat ggcatacact tttgccctga aatattcctc ctccaaaccg
     6481 gtaaatgaag cccaggcgct ggaattctac aacacgggaa agtaccacgc cggcctgtcg
     6541 ggtagcattt ctctcctcaa cgagctgaac tttgccatgg cacattacta caccaagaga
     6601 ggcaatacgg gtcaaagtga aaaatatctc aaccagattt ccttctttgg agggagcaat
     6661 tttgaacttt atttgaaaaa ggaaatggct gaagcggcca attttggagt cagagataag
     6721 cctttcgaac agctgaagtc tttacttgac gctatgtata aggcttacga attgggtcat
     6781 gattccattt atcaggcatg cctgcttaac atttccgact tttattatta tgaacaacaa
     6841 tacaacaaag cactggacta tatcgatcag ttgcagaaca ccatagccgc aaagggaata
     6901 caaactgact ccaaccgaat atacttcctc atggcgaata aattgccgat attcaacaag
     6961 caatttgacc agaatgcaac aatcaggctg gcagaatcca tttcccacta tgccaggaaa
     7021 catggctatc aattgctgga agagatagct cagggccagg tacggactgc cttgattagc
     7081 agtggtgact ttaagcgcgt ggaagactat tacagagctt accctgtcta cttgtccagg
     7141 cttgagcacg aaaagccttc tatctattac aggatgctgt cctatattga gatggcaaag
     7201 ggtaaaaaag acagcgcggt ggcctatttt gacaaggcgg gtgagttttt ggatggggaa
     7261 aatgaatatt atgtagctaa ttaccatttc agaaaagggg agttgatgga aaagctcggt
     7321 cagcgatcag aaagtatttc aagctatagc aaggcactca aaatgctcaa caatagtcac
     7381 gacctgacac tccagaatac tttgatcgac aaattgattc cgtattacaa ggagattaat
     7441 gacccgagat ccattgtcgg tttgttggaa aagaaagatt cgctgaacat gaaactgatt
     7501 caaatcaata aagagcaaaa tatccgccag ctggaattga gtaatatcct ctccgtatac
     7561 gaattgaagc atcaaaaggc ggctgaagag ctcaacagaa agcaggtgat ccagttgcag
     7621 attgtagcct tgttcatttt actggtattc atgagccttt tggtgatggc caatctgaaa
     7681 cttcccggct ggtggcttag atcaatgggc tatatttcca ttattatctt atttgaattt
     7741 attattctga tggtagacag gagactgcac gatatgttgc acggaagtgt aatcaaattg
     7801 ttggtcatta aagtcatact gatatcctgg atcttacctt tgcaccattg ggctgaaaag
     7861 aaaattgtcg cttttctttt gcaaaaggac aggttcagag gtcttaagga tttcctgtcc
     7921 aggttatttc aatcctttgg aaaaaaagaa gttgaagaat cgtgacttcc gattgtcgtt
     7981 gtacatcatg aatggattgc ccctgcaact tgcatgttga caggattact tcttatgcct
     8041 gatgactcgt cttgtgttgg aacctttgat tgtatttgtt ggtctgtgtt tcttgatcga
     8101 tctgattaac aacaagcaat gccactcaat gagcaggaac cgggatacct ctcccgagat
     8161 gaaaatcaac accgaaaaca agaaaagaac attcctgact ccagatacgg gatcgatgag
     8221 gtcactgtaa aaaggcatga taaccaaggc ttcaatggcc atcaggatgg tggtagtaat
     8281 cactccggca atgaaaaata cccgggtggg aagaaccagt ttacgactgc ggttcatcat
     8341 gatttccttg aagaaatacc tgacatttgg ctcatccgga ttcatggaca aaagctgccg
     8401 gatgatgctg agtgaatcct caaccctgcc tgcttcgttc agtgcgacag cttttttcat
     8461 taaaaggttg aaataaatat cgacaccaaa caaccttgaa aagcccaact ctattgcggt
     8521 ttcgatagca tcatcgacca ggaaaagtac ttctttgaat ttacccatct caaacagtgc
     8581 ctcgatgtaa cacacataaa tctcaaaacg gtgtcttgca tccaaatttt cgattttgtt
     8641 gcggtggtct tcatagaaat gaatcacctc tcgatatgat ccggagccaa tcgtcaaaaa
     8701 acggtcaaag accgaagggt catatatcga agtaaagtcc attttacctc aacctgtata
     8761 aaaacggtct gctgggagat acatccaact gaatatttag gagatgtaaa tttaacaaat
     8821 aaagtccatc ttcaacaaaa ttttctgcaa aaatcaattc agcaaccgtt gcgcccgccc
     8881 gtaaatcaat atccggataa ttccaaaatg ccctgtaagc ttctaattta ccctgatttt
     8941 cctcccgatc caacgatggc aggtcggtta agagatgctt tataccacgg ctgacgatca
     9001 ggtcgatagc atcacgactg aaataaggcg gattggtatc tgtgtattca gtctttctct
     9061 tttccggccc attgggcaaa gtccttatga tcaaggcttc ctcaccaaca gcctcccaca
     9121 attgcgataa ttgttctgca tgaatgacct tgtcgccatt atctgataat tgaggaaaga
     9181 tactcaccag ctgtgcaaca tggaagtccg gcttgtagta ttcattgata taatattggt
     9241 ccctgacgat atggccgaca cattgggtgt gcgtgccatt gccgtgagga tggatggtga
     9301 cattataaaa attggcgtgc cctccctgag ctatactttt gaccagatcg ccttctcgat
     9361 gtggggacgc atagaagtat ggagcccgga aacaattgac ctgcggataa ccactatgca
     9421 ggatcattga gatatcaatg gggtggtgga gatcagctgt atatttctga ccatttaagg
     9481 ttacggacac ttcgatatta taagtttcaa cattcacgac attcggattc ttgtattgtc
     9541 ggattacttg atcggtctat catttaggtt acaaaaaggt tatatcccgg atagccgcaa
     9601 tattaactgt acatatagtg atataacaac gcatggaaga ataaatagtt aacgtttatt
     9661 tttaaacata attcacctaa ctttggcgtt tatttcggga tagatagaaa tacggaacag
     9721 tatttttctt tctactttaa aagcaaagaa tggcaaagcc ggtatttatt gtaatcgagg
     9781 gtattgatgg gagcggtaaa tctacccaaa ttgagctgct ccaacataga atgtcagaag
     9841 aagggaagca ggttgcgatc accggtgaac ctacggatgg ctttatcggg aagatcatcc
     9901 gggacatcat gcgcggcaag gaagagaccg acccggcggt cgttgctgca ttgtatgtag
     9961 ccgacaggat ggatcacatc acccatccgg atgtgggcat gcttgcactg ttggagaaag
    10021 gatacaatat tattgccagc cggtattatt tttcaagcta tgcctttcaa ggtgaatatg
    10081 tttccctgga gtggctcatc catgccaaca gtatctgcaa aagccgcctt aaagctgacc
    10141 tgaccgttta tatcaacatc gaccctgcaa tatgttttga acggattaaa agtagtcggc
    10201 caagccttga tatctatgaa aatcttgaaa aaatgaagcg tacccaccgc gaatatatga
    10261 aggcttttga ggtggcagga atggatgaaa atattgtgat ggttgacggc accttgccga
    10321 tcgacttact gcatgaagcc atttggtcaa aggtgaaaga agtggctttc tgatcgaaaa
    10381 cgtagagaaa aaaatgtttg tcctgtaata acagcagttc tcatcaatat tattccagct
    10441 aatttcagct taagaaagta accgaatcca cgaacatccg cgttcaaatg accggctggt
    10501 gcagcctttc cccgcattga cggcagtaca ctgcatcgtt gctatgctcc ttggtgccac
    10561 aattttcaca gaccaggtta atattggtct tcttcatctc cttgatgatg ttaccgccta
    10621 tgattccggt cggtaccgca atgatggaat aaccgaggat catgatgaag gatgcaagta
    10681 tcttgcctat cggagtgatt ggagtgatat caccgtaacc tacagtcgtc agcgtgacga
    10741 tggcccaata aatgctctgt ggtatggaat gaaagttcgg attatgctgg tactctgcga
    10801 ggtacattaa agaaccaagc aggaaagcca gaatcaatat gaagaagaga aaaatggcta
    10861 tcttcctgaa acttctgatt aaagcaaaga ccagttctcg actttcatcc agaaacttaa
    10921 ccatgccaaa tatcctgaac acacgtaata atcggaaaga acggatgatc atcaacatat
    10981 gactttgggg aagaaaatac tcaaagtatg atgggagaat tgctatcagg tcaagcatgc
    11041 caaaagtact cttgacaaat cgctttttga ccttgacgca cgacaatcgc aacaaatatt
    11101 ccagcgtgaa aacaaacagg aaaaagcggt cagcttgtct gaaataaaaa gaatattttg
    11161 cactcaggga ctcaatactt tctgccagaa tgataaagat attaatcagg atcatcaatg
    11221 acagaatgat gtcaaacatt ttcccggcct tagtatttgc ctgaaaaatt ataacgtaaa
    11281 gtttatactg ccaggagttc ttgtcatcag gtctgttttg actctggaca acatcaattc
    11341 tatccggtaa tattctaaat aagctcaaat caaggttgtt tcaaaatcaa agtaaaggaa
    11401 tattacaatg tcttcaaatt tatacaaaga tagaatcttt ataattgcct gatatatgaa
    11461 ttttttgtaa ctattcacct taacataact taatcaggac atcttgatac caacttaaaa
    11521 ggaatcacca tgattaatca agggtcatga tcagaggttt ttttattcat cattagcaaa
    11581 cttgtttgcc tgcaactgct taatgcaaat gatgattgga tagatctttt tgtgtttttt
    11641 atgctctccg agaataaaaa ttgatccgat aaatcttttt tttgatccgg tgatttagag
    11701 aagctattta gcatctttgc atagactttt ccagcctgta aacccttttt cccaaatccc
    11761 attttttgca aaggaccaaa ccttttgggt caagatagtt taataatgga taaatatcta
    11821 tctgatgtga ataattgcga atccgtacta ttaaatatta cttgctgcat tggcatgaaa
    11881 tgaaggcagg aagtagcttt tagggaaata tgatttttta taaaaaatta tatattattt
    11941 taatttatta tgtgtatttt tgtgagatta aataaataag cctgactgtc tataacgttt
    12001 ttaagattgt aatgcaaaga ataaacggat cgattaaagt tagaaacact gatattcagg
    12061 gttatttgcc tattcgatat tctcccctcc ttccatttcc aatattcaat ttatgaggat
    12121 ttttattctt tcaataattt gttgcatatc atttgtcttt gctttaccgg ctcaacaagg
    12181 agtctataat atggttgatt tctataggga gctttcggtt ggacatcctg ttttgccctt
    12241 tccggaaaat gttaagtttg aatcagggct gaataaatac atcggggaac caatggctct
    12301 attgggtcaa agggcccccg gttcaagatc tggacaacaa aatgagatgt ccgccgtgac
    12361 aatgacggag attgagcatt tattcagtgg caggattcaa cgaggagttg ttcattctgc
    12421 caatcagcct ccggtagctg atgccggtcc ggacaccacc attgttctgc cggtcgattc
    12481 cgtccttttg aatggctcag gtaccgatcc ggatggaacc attgtaagtt tcgaatggac
    12541 tccagatcca aacaatccgt cagccgctac tttcgatgat gcttctctgc ccaatccgat
    12601 ggtaagcaat ctcattgaag gaatttatat tttcaccctg acagtaaccg atgatcaggg
    12661 agaaatcggt tcggaccagg tcacaataac cgtacaggct gctaatcaac ctcctgttgt
    12721 caatgcaggg gatgatttaa caatacactt gccggaaagt tccgtgcaat tgactggaac
    12781 agcgattgat cctgatggat ctgtcgttag tttttcctgg agtcaggatc cttcaaaccc
    12841 ggccccggca gccttttcga atttttcgat tcctaaccct gtggtcagcg gtttgatcga
    12901 aggtgtatat agttttacac tcactgctgt cgacaacgaa ggtgcgaccg gccaggacaa
    12961 tgtcctggtg aacgtccttc cgcccatcaa tctgcctcct gatgcccatg tcggttttga
    13021 cctgcgagtc attctcccca ctgattccac tattcttcat ggcagtggca ccgatccgga
    13081 aggtgctccg atcacttttt tgtggaaaca aatcggcgat ttgccttcga ttgcaacgat
    13141 ttccgattcg gcgagctcag atccggtagt gagtgatctc atccttggga tatacacctt
    13201 ccgattgatt gtgacggatg acaaaggact gtccgactcg gtagaaatga atgtcatagt
    13261 ggatatcgtg ggcaatatca aacctatagc taatgctggc gaggacgcta ccatattggt
    13321 accgcagacc agctaccagc tcgatggcag tggctctgat tctgatggat tcatcatcgg
    13381 ttacaactgg gaacagattg gtaccttgcc caacacagca atcatttcca atccgggtgt
    13441 tcccaatccg attgtttcag gacttatcaa gggagcctat actttccgat tgacggttac
    13501 ggacaatgaa gggggaaagg gcatgaatga tatggttctc atcgtgtttg ataccttcaa
    13561 tattgctccc atcgccaatg caggccccga tgccaccatt gcccttcctc aggacactta
    13621 tcaactaagc ggaagcggta ccgacgctga tggcataata atttcatacc attggcaaca
    13681 ggtcggcaac accccttccc aggcggtttt ttccaataca gatattccta atcccgttgt
    13741 gtcaggattg atttacggac actacacctt taggctgact gttaaggacg attatggtct
    13801 tacggcttcg gacgacattg tgctttttgt aagagatagc ttcaaccata ttccggttgc
    13861 caatgcaggt gaaaacattc gactgacctt acccgacaat agtgtccagg tccatggtac
    13921 cgggtatgat gatgatggaa tcatcgttgg ttatgaatgg caacaagtag gtaacaaacc
    13981 aagtaaagcc gtaatttcca atccggatat agccgacccg attatcggag gttttctgta
    14041 cggcacttat acctttgtat ttacagttcg ggacaatgaa ttcgctaccg gatcggacac
    14101 gctgctgatt gtggtggaca cggttccatt aatagcgccc atcgctgatg caggtgctga
    14161 tatttccgtc aatctgcctc aggacagtgt tcagttgcat ggcagcggtt cggatgagga
    14221 cggcaccatt gtaggctata cctggtatca attagggaca caaccctctc cggcggcttt
    14281 ttcagataca atgatcgctg atcctattgt aaaaaatctg gtcgaaggca cctatcattt
    14341 tgtattgaag gttaccgaca atgacaactt aatggatacc gatacggtaa gggttgtcgt
    14401 cagcaaggct aagaatattc ccccggtggc caatgccggc gaggatataa cgatcaagct
    14461 gcccattgat gcaacgaccc tgcatggtag cggaagtgat gacgatggta cagtgacaga
    14521 ctttctttgg gagcaggtag gtactactcc tgatattgca accatcatcg ggaataattc
    14581 tcctgaacca actgtatcca atctcttcaa gggaacgtat acctttagac tgatagttac
    14641 agataacgag ggtgccaagg ataccgatga ggtcaaggtc tttgttttgg acaaactgtc
    14701 tacccatacc ggggatgttt cttcggccat ctatccgaac cctgccaaag gcggcatctt
    14761 ccatattaac ccgggcgcca aattaaagcc tggtacatcg gctcgcatat ttgacataga
    14821 tgggcgggag atgatgcgct ttgtcgtagg ccgggaagat agctcctttc aatgtaatca
    14881 atgcgtatcc ggtgtttttg ttattcggtt tgataacggg atgagtgccg ctttggtcgt
    14941 tatgccttaa gaagtgtatt gtaaagttga cggcgtgtaa cctaaccaca gagcaccaaa
    15001 agaatccggg tatttgttct ttgtgccggc ctgtttcaat gttataccaa gatgatttat
    15061 ataatagtcc ataaattttt ggtattagag gctaaatggc tataatattt tcaaatacct
    15121 gaaaatcaac gtctgtgtaa ttaagtcccg gaatagtttt cagtcaatgt tcatgatgca
    15181 tctctaattg atcaagctcc atataagggg cacatctttc aaagaaacat gatcagagaa
    15241 attattcagg taaatattga tagaaaattg aggtagtaga tgtgactggc agcaatttat
    15301 aggaggctgt gacgatgctt ccatttcttg ttgcattgaa attccggtgc gatggagggt
    15361 tcacagtcta ctttggtttt ttgattttca attcatgata tcaattaatc gaagtaatga
    15421 aatataccgg tagagaatga gcttttcccg gatgtgagaa taatgcagtg gtcggacggg
    15481 tcaataaaat gattgatggc cttccccaat ttttcttact tttgcagcgt tgatttgtaa
    15541 gtgatgttaa ccatacagga tattcagggt tcagacaatg tgaagaaaga gattacaaca
    15601 ctgatttcaa gtggtcagtt tccgcataca accttgttta taggccccga aggcagtgaa
    15661 aaattaaatc tggcatacgc aactgcaaga ctgctgatgt gtgaaaacaa caagaatggc
    15721 gtcatatgtg gacaatgcaa ttcgtgccgt aaatctgcca attttaccca tccggatatt
    15781 ttcttttcat tcccatttgt gaaggaggga agcggggatt tccaatccga tgacttcctg
    15841 caggcatgga aagaggcatt ccgggaaaat ccttatatga atgtatatga atggtcactg
    15901 aaacaggatg ccggcaataa acagcccaat atcaccgcat tcgaatgtat acaaatacag
    15961 cgtaaactac agaagaagcc atatgaatcc gaccggaaag tcttactcct gtggatgccc
    16021 gaatatctgg gtaaggagag caatcgcctg ttgaagatac tcgaagaacc acccggtgat
    16081 acctttatca ttttggtagc tgaagaccag gaacgcttgt tggctacaat actttcgcga
    16141 tgtcaactct tcaaggttga gccattcgac ctgcaaggtg ttcagagaat attggaaaat
    16201 cattttggca aatcgaagga ggaagccact atggcagcat atctgtcccg aggaagcatg
    16261 gtagaagcac tgtcgctctt cagcgaaggt aacctggaga aaaacggtga attcgtgacc
    16321 tggctccgcc tttgctattc cttcaatcat gtggaaatca tacgatggat agaacacttc
    16381 gctacatata caagggaagt tcaaaaatat atttttaaat ttggcattca tttcgtcaat
    16441 gaaatgctga aaatcaaata caattatggg gtggacaaca tcagcctgcc aaaggaaatg
    16501 gccgatgtag cagcccgtct ttgtccactg ttgagttttg aaagcattga agagctggtc
    16561 aacacactgg acagagaaat ttttttaacg gagagaaatt gcaacacgaa gatgcagttg
    16621 atatatacat cgggttatat acaccgattg ttcaacggaa agcggttcgc agcgtgattt
    16681 ctcattaaaa tatattaatc atgggttgca atagttgcag taccgaagat ggcaagccca
    16741 aaggctgtag gtcaaatggg gtttgtggca cttcgggatg taataaacta aatacatttg
    16801 actggcttac tacaatggaa ctggaagacc ccacagaggt caatctggtc gaagtaagtt
    16861 tcagaaaagg tgcgcataag tcatttttca gacttcagga tgcgtatcag gctgagattg
    16921 gcgatgatgt cgtagtagat gcggatggag gttatgatat aggcaggatt tcattgaaag
    16981 gagccctggt tccgattcag atgaagaaga aatcagtaga tcctaattct gcccttagat
    17041 ttgtcatcag aacggccagt cagaacgaca tggcaaaact tgagcaagcg ataagtatga
    17101 atcgcaatac cctcatccga tcccgcgcta tagcagctac gttaggcctg gatatgaaga
    17161 taggagatgt cgattatagg gcggatctca agaaagccac attctattat acagcagacg
    17221 gaagagttga ctttcgggaa ctgatcagac attttgccaa ggaattcaga gtgaaaatag
    17281 agatgcggca gatcggttct cgtcaggaat cgtcaaggat aggaggcatg ggctcctgtg
    17341 gtcgggaatt gtgctgttcg acctggttga cagattttaa gtcggtcaca accaatgcgg
    17401 cacgctatca aaacctggcc atcaatcagg ccaaattgtc cggacaatgc ggacgcctga
    17461 aatgctgtct caattacgag ctcgatgtat atatggaggc cctggaggac ttccctacta
    17521 atgctgacgt gctcaggtat caggggggac aggccaggct catcaaaacg gatatcttca
    17581 aaaaattgat gttctatatc aagaaagatg aaaaaggaag ggaggcgttt atcacactga
    17641 gagtagacga agtacttagg attcaggaag atattgccca tggcaaattg cctgaagaca
    17701 taacatctac caaggtggaa gcctttgagg atgatgctgt tatcaaatct gaagatctga
    17761 caggacatat tgaattgccc gaagagaaga gaaggaagaa aaagaaaaag aagaatcccg
    17821 agaacaagcc tatgaggcac gaaggacaga aacaggagga taaaaaagaa accggcaatg
    17881 aaggcaagga taacaatcaa cggagacagg ataaccgcag gaacgaacat cgggataata
    17941 gaaacaaagc aggaaggcct caacaaggca ggtctgacca accggataaa aaaccggaac
    18001 cacgcgacaa aggtgtagac aggcctgaat ccattgaccg gaatcaagaa aaatccaata
    18061 atcagaataa gagaaataag aaacacagaa atcaacaacg caggtccggt aatcgcccca
    18121 acaacggcaa tgctgcgcct cctgccgatg cttaatttga ataaaataaa ttaaaagata
    18181 aacatgactt acgacgtagt agtaattggt tcaggcccgg gtggttacat ctgcgccatt
    18241 cgttgtgccc agttgggctt gaaaactgcc atcattgaaa aatacaagtc actgggaggc
    18301 acttgcctta atgtcggctg tatcccatct aaagcactgt tggactcatc ggagcatttt
    18361 ttcaatgctg cacatcagat ggcagatcac ggcatcaata tcggaaaagt ggagatggac
    18421 gccaaaaaga tgttcgaacg taagaaaaat gtcgtggata cagtcagcaa aggggtgtcc
    18481 tatctcatgt ccaagaataa aattgatgta cattatggag taggttcctt cattgattcc
    18541 tcaaatatta aagtaaatgg agaggaagga gaatccacac tgacgagtaa gtattttgtc
    18601 atcgcgaccg gctccaaacc tatcacacca gcagcattca attatgataa gaaacgaatc
    18661 attacctcca ctgaagcgct tgttttaggt gaaataccta agagtatggt tatcatcggt
    18721 gcaggcgtga tcggccttga actgggatca gtatatgccc ggctcggagc aaagattgat
    18781 gttgttgaat ttacggataa gatcataggt accatggatg tcgattgtgg aaaagaattg
    18841 cagaaagtcc ttaaaaagga aggatttact ttccacctta atcatgccgt tactactgtc
    18901 aaatccacca aaaccaaggt aaccgtagag tttaagcaga atggaacgga tgaagtcaaa
    18961 aagatagaag ctgattattg cctgattgca attggaagac gtccctatac agatggcctg
    19021 aatgccgaag cagccggtgt tatcgtcgat gctaaaggtc gggtcgaggt gggcgatcac
    19081 atgagaacca atgtgcccaa tatttttgct atcggcgatg tagtaagagg tgctatgctt
    19141 gcccataaag ctgaagaaga aggtgtcttt gtggcagaga ccattgccgg catgcgacca
    19201 catatcaatt ataacctgat tcccggtgtg gtatatacct ggccggaagt agcctccgtg
    19261 ggcgagacag agggacagct gaaagaacgc ggtgtggatt ataaagtcgg taaatttcct
    19321 ttcaaagcat tgggaagggc gaaggcaagc ggtgacgaag ccggctttgt caaggtgctg
    19381 gcagataaaa atacggatga aatcctcggc gtacacatga taggcccaag agctgccgat
    19441 atgattgcag aagccgttgt tgccatggaa tacagagcct cagcagagga tataggcatt
    19501 atttctcacg cacaccctac ctataccgaa gcatttaagg aagctgccct ggacgctaca
    19561 ggaaagcgtc ctttgaatat ataatttttt gaggagggtc aatagttagt gagctaaatg
    19621 gtgtagctgc cccgtttgct ttgcataagc ttacatcgca ttaatcctcg ttatccatga
    19681 accggacttt attccggaag tattgaattt cattgctatt tcaatggata gctaacgata
    19741 tcttatacca aggtgattta taaattgtcc ataagttttt ggtatccttg tccggacagc
    19801 tatactacca gagctctgct ctgtcccggg agtgaaacaa tccggggttc cgggatgccg
    19861 gggttatagt cgtatagatc aatgaaattg gtcaataaca aatgtacctt tggtattata
    19921 tggcagatac catttttgca aaagggatag ggctttgttt cttccccttt tgcaatctca
    19981 tcatgccgga gtttttttca gtttcttacc aatccatttg gcacttgata gaatcaatac
    20041 caacaccatt ccaatcgtga taccgaaagt gagttctttc aaaagaatcg gaaactttgg
    20101 ccacaaatgg tgaaaataat cgatgtaatg tacaaatatc cctcccgata ctgctacaag
    20161 tgcaatggtt cccactacgg aaagcatttt gatgacgtat ggcaatgctt tgacaagcag
    20221 gttaccaatt ttggacctcc aactgtttga tttagaatgt ctgatcagga aatatcctgc
    20281 atcatccatc cgtacaatca tggcgaccag accatacaca ccgatagtag ccaaaatagc
    20341 cacaaagctg actgttagga cctgaatgac aagatctttt tccagaaccg ttcccaaggc
    20401 aataattaca atctctatgg aaaggataaa atcggtggtg atggcggcct taatcttctt
    20461 tttctctgaa tcctcttcga tattatgatc agtttcttga tggatgtgat gacctttatt
    20521 cctgtgaaaa aaatattcta ttattttttc agcaccctca tatgccaggt agcaaccacc
    20581 cgcaatcaga atgtacttga taactaccgg gaaaaaaaag ttgaaagcaa gtgcaatggg
    20641 cacaataatc aacttattga taaatgagcc ttttgtaata gccagaagca ccggaatctc
    20701 tctcgatgaa acaaatcctg ttgccttttc cgcattgact gccagatcat caccaaggat
    20761 accggctgtt ttctgtgttg cgaccttgct tgccaaagcg acatcatcca tcaatgcggc
    20821 gatatcgtct aatactgcaa aaatacctga tgccataatt tagattaaca ctgggcgaag
    20881 ataaatattt gaaaaattgt atttataaaa aaatatacag tcattacttt cactgccttt
    20941 ccacaattca ttcgcagatc gataggttat ttatacaata aaaatgacag gattgtgcaa
    21001 tctgatttta ttggtcggat cagatatgtc ttcctttgtt tctgaatacc caaatactgt
    21061 aaaccagaaa aaatggaaat agtatggaac ttacgatggc cccgactcca aataagacca
    21121 tttcattctt caggtagata tcgataggga tttgtagttt atccggcagg tgcccaaaaa
    21181 atggttgtgt gccgtgattg tcaatatatg ttctgaaatt attgaattga tcaagcagca
    21241 aaaacgaaaa tggaaaggct gtagccaaaa aaaatagctt gacaccaaca tgtaaggtca
    21301 atatggaagt agaaacaaaa aacatgaatc ttgcataggt aaacagggca aaaacaaaga
    21361 tgaaattgtc caggtagaac ggaaactcac gagcatgggc atagattgga atcatgacaa
    21421 tcaaagccaa aatagttatt ccggctaccc aaagcatttc gaggaggatt atccttgttg
    21481 tttttacacc cactgttgtc tgttttccat ctgcaaaagt atctttattt tgtcatttgg
    21541 gcaaaaagtt cgttgatgcg ctatttcctt ctgcctgaag caattaaaaa tgggccctgt
    21601 caggcaactt tcagaagagt tactttattg tatcaaagaa accaatttgt tgttactgtt
    21661 gattcttttg aatgtgatcg cagttcatct atccttccca agcttagtat taatcctctt
    21721 gagcagtgat tgcctgaaga tgctcctgac agaatggtgc tttggattgg aaggtcatta
    21781 tctcccgggt cgtaggatgc ttgaaagtaa tcgtttcggc atgcaagtag agtctgtcct
    21841 ttctggttcc ataaaggtca tcgccttcga taggtgcgtt cagcccaagg tgatgtgccg
    21901 catgcaccct caattgatgc gtccttcctg ttaccggatg gaaacgtatg atagtcttgc
    21961 catccacaat attgagaatc ttgtatgttg taagcgcatt
//
LOCUS       DAOQMC010000006        21333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_846_length_21333_cov_4.373766, whole genome shotgun sequence.
ACCESSION   DAOQMC010000006 DAOQMC010000000
VERSION     DAOQMC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21333
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_846_length_21333_cov_4.373766"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PKV31_00585"
     CDS             complement(<1..771)
                     /locus_tag="PKV31_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB91371.1"
                     /translation="MAYKEYKSLNLTEIDSEILKFWQSNKIFEKSISERDGAKPFVFY
                     EGPPSANGQPGIHHVMARTVKDIFCRFHTMLGEKVERKGGWDTHGLPIELNVEKELGI
                     TKDDIGKSISIEDYNAKCRETVMKFKDRWDDITQRMGYWVDLDNPYITFDNNYIETVW
                     YLLQMLYTKNYLYKGYSIQPYSPAAGTGLSTHELNLPGAYKEVKDTTAVALFKVIHDD
                     NANFLFETPDEDVRFAAWTTTPWTLPSNTALAVGEDIVY"
     gene            complement(970..2283)
                     /locus_tag="PKV31_00590"
     CDS             complement(970..2283)
                     /locus_tag="PKV31_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB91372.1"
                     /translation="MKQLYMVVLLIWFQKSGAQNLYFPPITGNQWATVTPSELGWCDH
                     EIQPMYDYLEATHTKGFMVLKDGKIVLEKYFGSFNQDSVWYWASAGKSLTAFVIGVAQ
                     QKGLLSIEDKVSKYLGEGWTSCTRDQEDKIRIRHLLTMTSGLDDLVPDLFCNAPECLK
                     YKADAGTRWAYHTGVYSLLLDIVEKITGKNINNFLNQNIAWQIGMKGLWTSSSYGELY
                     FTNIRSMARYGLLILNKGVWDGKDLMKDSEYFNAMINTSQQLNPSYGYLWWLNGKSGY
                     LLPSLQITFPGSVIPNAPDDMVCALGKNGQIICVIPSLNIVMLRMGDNPDGADGMITP
                     TYLRNIFDYFNLVACNTSSSRQSLLPSEITIRPNPAHNYLTWSIPDETVYQQVDITDI
                     HGRVHLSRQKTSGTLDISNLSNGVYFLVLKDKNGTILGSRRFLKL"
     gene            complement(2498..3904)
                     /gene="dnaA"
                     /locus_tag="PKV31_00595"
     CDS             complement(2498..3904)
                     /gene="dnaA"
                     /locus_tag="PKV31_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNB91373.1"
                     /translation="MSVNHNTVWKNCLNLIQENISEQGFKTWFKPIVPIRFEDQVLTI
                     QVPNQYIYEMLEDQFIGILKKAIKSTIGPEGKLEYEIPVNKMPEASNTSSAETVGLQD
                     MDSARIKNPFVIPGIKKAAFDSELNPKFIFDNLIEGECNKMARSAGLAIAERPGITSF
                     NPLFLYGGTGLGKTHLAHAIGNKIVEKFPSKRVKYISCEKLTNQVVYAFKTGTISDLS
                     LLYNSFDVLIVDDIQFLSEKPRTQETFFFIFNELHQNQKQIVITSDRPPKDMEKMEKR
                     LISRFKWGLTAEMELPDYETRIAIAESKIEAEGFKVPEDVLEFICYNMQSNVRELEGM
                     LISLAFASSLNNREINIQLAKEIINKFVDQLSKEVTIENIQSLVAEYFKMPIEKLSGA
                     SRKRSIVIARQLSMYLAKNFTNKSLKTIGENFGGKDHTTVIYSCKAVQDMIDTDPEFK
                     GTVEELERKVRTSLAVSH"
     gene            4659..5795
                     /locus_tag="PKV31_00600"
     CDS             4659..5795
                     /locus_tag="PKV31_00600"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNB91374.1"
                     /translation="MTDKLAKYGASIFSTMTAKAIQHGAINLAQGFPDFPVDERLIDA
                     VTEAMKSGYNQYARPSGAPELHREISSIIASKYSRIVDADRELTVYPGASTAIYIAIQ
                     AIINPGDEVVIFEPSYECYVPTIEICSGIIRPVRLVPPEFQPDWEEVERLVSDRTRLV
                     IINNPHNPSASVWRQEDFRKLEKILEGRDCLILSDEVYEFINFTEEGHLSIHSMEGLR
                     NKTLVISSFGKSLHVTGWRTGYCVAPPDVTIAMRKINQFSTFCANHAMQVGIASYLRD
                     YFDASRIRDTYRAKRDMMVNGIIDSRWKLLPCRGTYFMLLDYSSISDLPSMEFSEWLV
                     THKGIAVIPCSPFYSEGYDPRIIRICFAKKEETLIKAIDLLCNI"
     gene            5783..6550
                     /locus_tag="PKV31_00605"
     CDS             5783..6550
                     /locus_tag="PKV31_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNB91375.1"
                     /translation="MQHLNVSLVQYDIIWEDSNANLEYLDTLLETARGSDLIILPEMF
                     NTGFSMDAATVAQKDDGQAVSWLCIRSGELETTIMGSVAIREEGRYYNRCYIASEGEI
                     IAQYDKRNCFTYAGEEKIYTSGNSQVTFEVKGWKVKPLICYDLRFPLWCYNRQEADLM
                     VFVANWPESRIIHWDVLLQARAIENQAFVIGVNRLGSDGTGKAHNGHSAIVGPYGNYV
                     TGPLTKAGVSHARLDPSEISRYRAMFHLLDEERMKSE"
     gene            7119..8567
                     /locus_tag="PKV31_00610"
     CDS             7119..8567
                     /locus_tag="PKV31_00610"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNB91376.1"
                     /translation="MADCNLAPLSPYVPTAEKPWNEQRVKHLYARIGYGITAAEIPDI
                     LSKNPVEYIESLVDEAFNLPVFPKPDWADWKPGQFNEEDGTEHYIIYTQFVTDWINEK
                     LKRPLRDKIALFWSNVLVTKFEDYYYAPFLYQYVTNLQRNALGNYRTFIREMGLEHSM
                     LIFLNGFQNNKWQPNENYARELFELFTLGLDNGYTENDIKETARAFTGYNGADLENYI
                     IYFTPFTHDNGLKTIFGETANFDYNGVIDLLFEKKGDLICRHICGKIYRHFVNAEGND
                     NIIQQLADIMLANDFELLPVFKALFCSEHFFDATEFNTHIKSHIEYFTGIIKQFNYTL
                     NYEEGNAMIWYSSSLGQTYFDPVDVAGWKEDKSWIDSGSLLVRWLYSEWMYGGLIWQN
                     QDFVVSYLKNLTNNSNDVEFVVRTVVDQFFVSGLASQADYDRLLIAFKADLPENYYEQ
                     GLWNLDWEKDFICWQTNLLLNEMARLPDVTLC"
     gene            8599..10221
                     /locus_tag="PKV31_00615"
     CDS             8599..10221
                     /locus_tag="PKV31_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNB91377.1"
                     /translation="MNNKDIEKRGLKLEDGVLHEADHVEYSRRRFLKSFGLAGGLGFM
                     LGGLPVSASTLTRWSMPPILEKSDRILIMIRLKGGNDGINTIIPLDQLSTYHNARPSI
                     GYQDNVMIQLNDQIAMSPHLAGLKRLWDEGMMKVVQGVGYPEQNLSHFRSSDIWSSAS
                     DSNEVVRSGWVGRYVEETFPDYVENPPVYPPAIQIGGASSILFTGDHIGKLGFEAFSG
                     AQLEDFATTGNAHSLQDLPPCSYGDQLRYVRIITNSTYKFVEAIKTAYSLGPETTLTY
                     EDERFPESLKIIARLIKGGLKTKIYVVELDGFDTHSNQIGKHEELLKQLAAAVDTFYK
                     DLEGTEYEENVLSVTFSEFGRRIEQNESPGTDHGAASVLLAFGKCLEGNGTLGKYPSL
                     TETDEDGNLIFNVDFRKVYNTLFTEWLCLEDAVSDQILLKDFGDPLDFGFTCQEVSSV
                     KTPAKPGFVHSASYGLGDVFVNYTIPKDVMVMVELVSMSGQPIVLERPMMKKAGSYSI
                     PLQQARRRLMPGAYVYRIRVTGADYTGKLIIN"
     gene            complement(10588..11079)
                     /locus_tag="PKV31_00620"
     CDS             complement(10588..11079)
                     /locus_tag="PKV31_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNB91378.1"
                     /translation="MIPLLMIVIGLVGIPTPRNYEEFKAAMLAEVNQYRLNGCNCGNV
                     RMKPVSALTWNDALAASAAEHAIDMHRQGYFDHKSRDGRNYPGRIKEKGYDWEIVAEN
                     IAYGQKNIAQVVSDWMASPGHCFNIMNPGIKQMGVAKKGGYWVQDLGSARENSLKARV
                     NLR"
     gene            complement(11480..12211)
                     /gene="truA"
                     /locus_tag="PKV31_00625"
     CDS             complement(11480..12211)
                     /gene="truA"
                     /locus_tag="PKV31_00625"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNB91379.1"
                     /translation="MRLLAFCDFVGTNYVGWQRQPNGISIQEAIEHALSIYFRSPIAI
                     VGCGRTDSGVHGRNYGFHFDLPEIPEDAPFLFKINRILPDDIVIRSIKVMEDNFHARF
                     SAKSRRYIYRIHTSKDPFIQPYSWYLDRLTPESVHILNEAATFIASLKDFSSFAKTGS
                     NNKTTYCDMIQCEWKAHEDRLELHIAANRFLRGMVRLIVGGCANVAVGKLSLERMKEL
                     SLEGVVLPFAFSVPAQGLTFEGVEY"
     gene            complement(12211..13449)
                     /gene="clpX"
                     /locus_tag="PKV31_00630"
     CDS             complement(12211..13449)
                     /gene="clpX"
                     /locus_tag="PKV31_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNB91380.1"
                     /translation="MAKNKDLQRCSFCGKDKNEAIILIEGLDGHICESCVEKAYDIIE
                     QEIFDKQPSDNLQVSDFAKRTPKEIKEYLDNYVIGQEQAKKTLAVAVYNHYKRLYQHD
                     KDVEIEKSNIIFVGPTGTGKTLLAKSIARMLNVPFAIVDATVFTEAGYVGEDVESILT
                     RLLQVCDYDVKMAEKGIVYIDEIDKIARKSDNPSLTRDVSGEGVQQSLLKMLEGTDVN
                     VPPQGGRKHPEQKMIKVNTQNILFICGGSFDGLDKIIARRLNTQIIGYSHEAKEKVDQ
                     SNLYQYAMHQDLKHFGLIPELLGRLPIMTYLDPLNLDMLKSILIKPRNAIIKQFIKLF
                     EYEKVELIVTDDALDYIAQTALNYKLGARGLRSICEAILMDDMYELPSKKNVDRLVVD
                     LDYVKTKLAGSRIEANLKVA"
     gene            complement(13524..14228)
                     /locus_tag="PKV31_00635"
     CDS             complement(13524..14228)
                     /locus_tag="PKV31_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNB91381.1"
                     /translation="MIPKDEFKKYATKHLNMNSMHLDKYMQVSVPNIHGFTPAVIEER
                     QMNVVSMDVFSRLMMDRIIFMGVPVNDYVANVIQAQLLFLESTDPKRDIQMFINSPGG
                     SVINGLGIYDTMQYVSPDVATICTGLAASMGAVLLAAGKKGKRTCLYHSRVMIHQPLG
                     GMEGQVSDMEISYKLIKKMQKELYDILAAHTGKDYDIIEKDCDRDNWMTSAEAKEYGL
                     VDEVLEKTNPSRLTQD"
     gene            complement(14386..15885)
                     /locus_tag="PKV31_00640"
     CDS             complement(14386..15885)
                     /locus_tag="PKV31_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNB91382.1"
                     /translation="MTINPVEITKNQLQLDVTMEAAEIQDLLKKELKKTAQKAKIKGF
                     RPGKTPVNLIKNMYGTQVYVDVINKNLFDKAQEYLKDNDINTFSNFVINDVNEDYKFN
                     IDKVEDFSASLVFVKEASLEDLDFDYSGKLTYYLPEISQERLDGQIKSIRYNIGGMAE
                     SSEPAGEESLISFDAVQLENGEVKEGGHTMTFNYSVSNIQSDDLNKNIIGKKIGETFD
                     INLKDFTDNEATLRNQILKLDSEEQNDTFDYEFRVTISNIQEKVLADMDEEFFTRLDP
                     RGNIKSEEDLIRLLKEDYHAYYRISSNMLFFHEFKEMALTETQLDLTDDFYKKFFTQV
                     SQLDDHALQYHLDEYKEDFKWAWIKDIMDKEYGIQVSRSDVNTALIREINEYFGGANL
                     PENIYQKFLEDAYKDNRHIARKKDEIYMAKLASFLIDEHGIQVVNIPTEEYNEKIEEL
                     NKKYEKQAEHHHHDHNHGHEHNDEHKHDGDFEVEEVEAKDADNTSGEEA"
     gene            16089..17888
                     /gene="lepA"
                     /locus_tag="PKV31_00645"
     CDS             16089..17888
                     /gene="lepA"
                     /locus_tag="PKV31_00645"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNB91383.1"
                     /translation="MKLIRNFCVIAHIDHGKSTLSDRLLEYTQTIAERDMQDQALDDM
                     DLERERGITIKSHAIQMYYHHDDGQVYTYNMIDTPGHVDFSYEVSRSIAACEGALLLV
                     DASQGIQAQTISNLFMAIDHDLEIIPVLNKVDMESAMVEEVSDQIIDLIGCKKEEIIL
                     ASGKTGIGVHDILRAIVDRIPAPKGDQDAPLQALIFDSVFNSFRGVIVYFRIINGIIN
                     KNDKVRFFHTGKEYFADEVGILQLNMVEKPQLTAGNVGYLITGIKVSKEIKVGDTVIL
                     AKYPATEEIKGFEEVKPMVFAGIYPIDNDDFEDLRDSLEKLQLNDASLTFEPESSVAL
                     GFGFRCGFLGMLHLDIIQERLSREFNQEVITTVPNVMYHAFLNKAPNERIIINTPNDL
                     PDPTLLHHVEEPYIHAQVITKPEYIGSIMSLCMDKRGILTKQTYLTTSRVELTFEMPL
                     AEIVFDFYDKLKSISRGYASFDYQPLDYRKSELVRLDIKLNGDNVDALSALVHKTKAE
                     ELGRKICAKLKDLLPRQQFMIAIQAAIGAKIIARETLSAMRKDVTAKCYGGDISRKRK
                     LLEKQKEGKKKMRQIGSVEVPQKAFLEVLKLND"
     gene            17899..18522
                     /locus_tag="PKV31_00650"
     CDS             17899..18522
                     /locus_tag="PKV31_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91384.1"
                     /translation="MKYFGILTCIALMSLAACKSESAGNAAKADDKEAYVAYDGQTRQ
                     IASVDTLVNNKGVQFELTFRGGSTDGAAQIKIRKDGKLIENTDRKADGRVTAAMIEDI
                     NGDGEGDFIFYTNTEDNTRIGNLYGVVSDNGKYRHIFISPFIKEPYVTNYFGRDSFYV
                     ENGIIYKSFPRYELNKLRQKVDSGKRWILQYKYTGKDTIDVSEGIIQ"
     gene            complement(18587..19261)
                     /locus_tag="PKV31_00655"
     CDS             complement(18587..19261)
                     /locus_tag="PKV31_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91385.1"
                     /translation="MQRKIQYFTTTLILLFYFFSLTDVHAQFGISFKYVLDYEDIPSN
                     IPGKANDRLSQQGSQVALDYRIKLVNYGVLFGPEIFAKMVRIKGIDIFDASNLEVGRV
                     KSIRSFGMNIPVTIYPFHFDQCDECPTFSRQHFFKNHFFLQPVVGYELRNWVNEPDNV
                     VPEINEHFAMAGLGLGLDFFAGKYVKISPILQYKHYFPLTDNSLYQSKLKPAAFEAGV
                     RIGWGR"
     gene            19442..20761
                     /gene="rimO"
                     /locus_tag="PKV31_00660"
     CDS             19442..20761
                     /gene="rimO"
                     /locus_tag="PKV31_00660"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNB91386.1"
                     /translation="MKTRTLEQNKVSVITLGCSKNLVDSENLITQLKGNDFDVQHDEV
                     TRDTDIVIINTCGFIDLAKEESINTILKYAKVKSKGGIDKLYVTGCLSQRYKDNLEEE
                     IPEVDAYFGTLELPRLLNTLEADYKHELVGERVLTTPSHYAYMKISEGCNRTCAFCAI
                     PLMRGGHVSKPVEELVKEAKSLARIGVKEIILIAQELTYYGLDIYKKRALPDLLDALA
                     EVEGIEWIRLQYAYPSKFPMGIIDAMKRHDKVCNYLDLPLQHASDKILQSMRRQITRD
                     ETKQLIRDIRQMIPGIAVRTTFIVGFPGETEEDFDQLCDFVREMRFERLGVFQYSHEE
                     DTAAYEMEDDVPESIKAERANALMAIQQEISLELNQQKIGKIFKVLIDKKEGGYFYGR
                     TEFDSVEVDNEVLIDASSNYCSVGGFCDVIIDDATEYDLIGHVIQPG"
     gene            20775..21191
                     /locus_tag="PKV31_00665"
     CDS             20775..21191
                     /locus_tag="PKV31_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91387.1"
                     /translation="MNKLYNYLFVRRHPLVWALAIILIFSILMVVNYYWIFPNNPEWM
                     FVAAFIGVLMYGVYSAFMLFITKKIMLDWNQSFIGFFLVAATLFYEGMLISDKSWDDL
                     SNYTPILKVILGVALIIMSMSIMARKVLEKMKQLDR"
BASE COUNT         5931 a         4330 c         4601 g         6471 t
ORIGIN      
        1 atatacaata tcctcaccta cagccagagc agtattggag ggtagtgtcc atggtgttgt
       61 cgtccatgcc gcaaaacgca catcttcatc cggtgtctca aaaagaaagt tagcattatc
      121 atcatgtatg actttaaaca atgctaccgc ggtcgtatcc ttgacctcct tatacgcgcc
      181 cggaagattg agctcgtgcg tagaaagtcc ggtacctgca gccggagaat atggctgaat
      241 ggaatatcct ttgtacaggt aattcttcgt atagagcatc tggagcaggt accaaacggt
      301 ctcgatataa ttgttgtcaa aagtaatgta gggattgtcc aggtccaccc agtatcccat
      361 tctttgggtg atgtcatccc agcgatcctt aaatttcatg accgtctcgc gacacttggc
      421 gttataatcc tcgatggaaa tggactttcc gatatcgtcc ttggtaattc ctaattcctt
      481 ttctacattg agttcaatgg gaagaccatg ggtgtcccaa ccgcctttcc gctccacttt
      541 ttctcccaac atagtatgga aacggcagaa gatgtctttg acggtccttg ccataacgtg
      601 gtgaatgccc ggttgcccat tggcacttgg cggaccttcg tagaacacaa atggtttagc
      661 gccatcacgc tctgaaatac ttttttcaaa aatcttgttg ctctgccaaa acttcagtat
      721 ctcactatct atttctgtca gattcagtga tttatactct ttgtatgcca tggaatgaat
      781 aatatttgga aaataagccg caaagataaa ataattaagg cttatttagg atatttatgt
      841 aggtatgagt agatagatcc ctgtatgtaa gcggaatcaa tattataaat ccacgcccgg
      901 aataatcgga caataccaaa ccaagaaaga tattttgatc cgtcctgatt ccaatcggtt
      961 cagactgaat tacaacttga gaaatctcct gctacccagt atggtaccat ttttatcctt
     1021 caatacaagg aagtaaacac cgtttgaaag gttggaaata tccagggtgc cggatgtttt
     1081 ctgccttgac aaatgcactc tcccgtggat atccgtgata tcgacctgtt gatagacagt
     1141 ttcatccggg atgctccagg tgagataatt atgagcagga ttgggtcgta tcgtgatttc
     1201 ggaaggaagg agtgattgac gggaagaaga agtattgcaa gctaccagat tgaagtagtc
     1261 gaagatattt cgcagatagg taggcgtgat cattccatcc gcgccatcag ggttatcacc
     1321 catcctgagc atgacgatat tgagtgaggg aatgacacaa attatctgtc catttttgcc
     1381 taatgcgcat accatatcgt ccggagcatt gggtatcaca gaacccggga aagtaatttg
     1441 cagagaaggc aacaaatatc ctgatttgcc attcaaccac catagatatc cataagacgg
     1501 attcagttgc tgggaagtat ttatcatggc attgaaatac tcactgtctt tcatcagatc
     1561 tttaccgtcc cacacacctt tattgaggat caataatccg tatcgagcca tggagcggat
     1621 attggtgaaa tacaattcgc cataggaaga actggtccat agacctttca tgccaatctg
     1681 ccaggctata ttctggttga gaaaattgtt gatgtttttt cctgtaattt tttcgacgat
     1741 atccagcagc aagctatata ctcctgtatg atatgcccac ctggtgcctg cgtctgcctt
     1801 gtatttcaga cattcgggcg cattgcagaa caaatccgga accagatcat ccagtcccga
     1861 tgtcatagtc agtaaatgtc gtatccgtat tttatcctcc tgatcgcgtg tgcatgaggt
     1921 ccatccctca cccaaatact tcgatacctt atcttcaata gatagtagtc ctttttgttg
     1981 tgcaactcct attacaaagg ctgtaagtga tttgccggcc gatgcccaat accagacgga
     2041 atcctgattg aaacttccaa aatacttttc caatacgatc tttccatctt tcaacaccat
     2101 gaagcctttg gtatgcgttg cttccagata atcatacatt ggttgtatct catgatcgca
     2161 ccaaccaagt tcacttgggg tgacagtcgc ccactgattg ccggtgatcg ggggaaaata
     2221 aagattctgg gcacccgatt tctggaacca aatcagcaag acgaccatat aaagttgctt
     2281 catagcttta tctttttact ttgacacatt tatcaataat tggtttatcg tcaggtagat
     2341 tttttttgct atgtgaaatg gttaatcctt tttttacaca tggaattcgg caaatttact
     2401 tttttttcat cgactattaa aaattttttt aattgtatat caataatata catattatac
     2461 atatcttaat aaagagtgat gaaaacggag tggctactca atggcttacc gccaggcttg
     2521 tcctgacctt tctttcgagt tcctcaacgg tacccttgaa ttcaggatcg gtatcaatca
     2581 tatcctgtac tgccttgcag gaatagatta cagtggtgtg gtccttaccg ccaaagtttt
     2641 ctccgatggt tttcaaggat ttgttggtaa agtttttagc gagatacatc gacaattgcc
     2701 tggcgatgac aatggaacgc tttcggctag ccccggagag tttttctata ggcatcttga
     2761 aatattcagc gactaaggac tggatatttt caatggtgac ttccttactc agctgatcga
     2821 caaatttatt gatgatttct ttggccaatt gaatatttat ctccctgtta ttaaggctgg
     2881 aggcgaatgc aagcgaaatg agcatccctt ccagctccct gacattggac tgcatgttgt
     2941 agcagataaa ttccaatacg tcttcaggca ccttaaatcc ttctgcctca atttttgatt
     3001 cagcgattgc tatccgggtt tcataatcag gtagctccat ttcggcagtc aggccccatt
     3061 tgaagcggga aatcaaccgc ttctccattt tttccatatc cttgggtggc ctgtcagaag
     3121 tgattacgat ctgtttctga ttctgatgta attcgttgaa gatgaagaaa aaggtttcct
     3181 gggtccgtgg tttttcgctc aggaactgga tatcatccac gatcagcaca tcaaacgaat
     3241 tatacaaaag cgataggtct gaaattgtcc ctgttttgaa agcgtagacc acttgattgg
     3301 taagcttttc gcaggagata tattttactc gtttggatgg gaatttctct acgattttgt
     3361 tgccgatggc atgagccagg tgtgtttttc ccagaccggt gcccccgtac aaaaagagtg
     3421 gattgaagga tgtgatgccc ggccgttcgg cgatggccag acctgcggag cgcgccatct
     3481 tattacattc tccttctatc agattgtcaa aaataaattt cggattcaat tcactgtcga
     3541 aagctgcctt tttgatgccg ggaatgacaa aggggttttt gatcctggcg gagtccatat
     3601 cctgtaaacc tactgtctca gccgatgaag tatttgaggc ctcaggcatc ttgttgacag
     3661 gaatttcata ttcgagcttt ccttccgggc ctatggttga tttaattgcc tttttgagga
     3721 tgccaatgaa ttgatcctca agcatttcat agatatattg attgggcact tgtatagtca
     3781 acacctgatc ctcaaatcgg attggaacta taggcttaaa ccaggtttta aatccttgtt
     3841 cagaaatgtt ctcctgaata aggttcaagc aatttttcca aaccgtattg tgattcacag
     3901 acattaactc gggcacttac tatataattt gctcattatc aatattcttg cagcaagtaa
     3961 agccctgatg caagtattac agtattaatt taaaggtcgg attttggcgt ggacggagga
     4021 caaatatcaa caaactttga cgtttgacaa acaaaaaaac ggattatttc tccaataaaa
     4081 tttacataca aaaatattaa atatatatta tttttaaatt taaaggttgg tgttgaattt
     4141 ttgttttccg acggtcaaaa ataggaaatg attgatgata ataaatataa tattttaggg
     4201 gaaatttaaa attggtagca ttggtatggg attatttgga ggaagtttaa atattatccg
     4261 gtacgaaacc gattaggttg ccggattggg ccggatgagg tgatggatta acaaatttgt
     4321 tgggccaacg tgaattaggt aggaaaataa tacttacaaa tgcgcttctt tagcggcttt
     4381 tgggatattt ttaagaatat ttttggctaa aagtctgata tgaagttacg gtcatttcca
     4441 gacacttgat cagaacgggt ttaccgggta ttactcatct acctacttcc atttggcccc
     4501 catcctatgc cggttttcag actataacca tagattttcg ccttgaagaa tgccattttc
     4561 tccttctgaa tttcttacaa tcttaccaaa atgcttattt tgcctgttct taatatgcaa
     4621 tcaaaaatcc gccatcgact tacttgcgat ataaatatat gacagataaa ctagcaaaat
     4681 acggtgcatc catatttagt acgatgacag ccaaggccat acaacatggt gccatcaatc
     4741 ttgcacaagg tttccccgat tttccggttg acgagcgtct catagatgct gtcactgagg
     4801 ccatgaagtc aggatacaat caatatgcca gaccttccgg agctccggaa cttcatcgtg
     4861 aaatctcgtc aatcattgcc agtaaataca gtaggattgt agatgcagat agagaactga
     4921 cagtctatcc cggcgcatcc actgcaattt acattgccat ccaggccatc atcaatccgg
     4981 gtgatgaggt ggtaatcttt gaaccatcct atgagtgtta tgtccctact atcgagattt
     5041 gcagtggtat catccggcct gtccggcttg taccgcctga gtttcaaccg gattgggagg
     5101 aagtggagcg tcttgtgtcc gataggacca ggctcgttat catcaacaat cctcataacc
     5161 cttctgcctc agtgtggcga caagaagatt tcaggaagct tgaaaaaatt cttgagggaa
     5221 gggattgtct gatcctgtca gatgaggtct atgagtttat caattttaca gaggagggac
     5281 atctctccat acattccatg gagggtctcc ggaacaagac gcttgtgatc tccagttttg
     5341 gcaagtctct tcatgtgacc ggttggcgta caggctattg tgttgctcct cccgatgtca
     5401 ctattgccat gcgtaaaatc aatcaattct ctaccttttg tgccaaccat gccatgcagg
     5461 taggtatcgc ttcttatctg cgcgattact tcgatgcatc ccggatcagg gatacataca
     5521 gagccaagag ggatatgatg gtgaatggaa taattgacag ccgatggaaa cttctgccat
     5581 gcaggggtac atactttatg ttgctggatt atagttctat ttcagacttg ccttcaatgg
     5641 aattttctga gtggctcgtt acccataagg gcatcgctgt cattccatgt agcccgtttt
     5701 attctgaggg gtatgatccc cgaattatca ggatttgttt tgccaaaaaa gaggaaacat
     5761 taattaaagc aatcgactta ttatgcaaca tctgaatgtc agtttagtgc aatacgacat
     5821 catctgggaa gacagtaatg ccaatctcga atatcttgat acattgctgg agacagcacg
     5881 aggcagtgac ctgatcatac tccctgaaat gttcaacaca ggattcagta tggatgcggc
     5941 aacagtggct caaaaagatg atggacaggc tgtatcatgg ttatgtatcc gatcaggaga
     6001 attagagaca accatcatgg gttcagttgc catccgggag gaaggtcgct attacaaccg
     6061 ttgttacata gcttccgaag gagaaatcat agctcagtat gataaacgga attgcttcac
     6121 atatgcaggt gaggaaaaaa tatacacctc gggtaatagt caggtaacct ttgaggtcaa
     6181 aggctggaag gtaaagccac ttatttgtta tgacctgcgc tttccattat ggtgttacaa
     6241 taggcaggag gctgatttga tggtttttgt cgccaattgg ccggagtcac gcatcatcca
     6301 ttgggatgtt ctgttacaag cccgggcaat agaaaatcag gcatttgtca tcggtgtcaa
     6361 ccgcctcgga agtgatggta caggaaaggc tcacaacggg cattcagcga ttgtcggacc
     6421 ttatggtaat tatgttaccg gaccattgac aaaggcagga gtctcccatg cccgtcttga
     6481 tccgtcggag ataagtcggt acagagctat gtttcatttg ttggatgagg aaaggatgaa
     6541 gtcggagtag ggaagttttt ccttgctatg ctactatgct atccggtcag acatactgga
     6601 cgattaagaa tcaatgttat tattttctct ggcggctcta atcgttccat gacttccctt
     6661 tatcagccgg taggaaaaac tttgcttaca catcgtgaat aaatgtttct ttgcgtgaga
     6721 tgggtgagtc gcggcgttga ttatcttttg aatcacaaaa taggacaaat aatgaccgta
     6781 aggtttgcaa aatagcgctg cacttaaagt gtttgattca gtgaaactct gaaatcatga
     6841 cctgaggtat acttcggaat cagatattac cggatgatta tatcctcttt caatcaaatt
     6901 agaactatga acattgaatt ccatctttgg aataaaattt cggatagtct tttcctttga
     6961 ggcattttgt ttgatattct ttttctctgt catggttttc attaggatgg atgtaagaat
     7021 attttttgaa ggaaatatat tttctttatt atttggacgt ttattatctt agcagcgtat
     7081 taagcatttc catatttttt catccttaat cattcgttat ggcggattgt aacctagcac
     7141 ccctgagtcc ctatgtccct actgcggaaa agccctggaa cgaacagcgt gttaagcatc
     7201 tgtatgccag gataggttac ggtattacgg cagcagagat tcctgacatc ctctctaaga
     7261 atccggtgga atacatcgaa agcttggtgg atgaagcttt caatctgcct gtttttccga
     7321 aacctgattg ggcagattgg aaaccgggtc aattcaatga agaagacggt accgaacatt
     7381 atatcatcta tacccaattt gtcacagact ggatcaatga aaagttgaag agaccattgc
     7441 gtgataagat tgccctgttt tggagcaatg ttctggtaac taagtttgaa gattattatt
     7501 acgcaccatt cctgtatcaa tatgtcacca atctgcaacg caatgcactt ggtaattaca
     7561 ggacctttat ccgggaaatg gggcttgagc acagtatgct tatcttcctg aacggttttc
     7621 agaataacaa atggcaaccc aatgaaaact atgctagaga gctttttgaa ttgtttaccc
     7681 tggggcttga caacggttat acagaaaatg atatcaagga gacggcacgg gcatttaccg
     7741 gttataatgg tgcagatctg gaaaattata ttatttactt tacacccttt acccatgata
     7801 atggacttaa gaccatcttc ggtgagactg ccaattttga ctacaatggc gtcatcgatt
     7861 tgctgtttga gaagaaaggc gacttgatct gcaggcatat ttgtggaaag atttatcgcc
     7921 actttgtcaa tgcagaaggt aatgataaca tcattcagca actagccgat atcatgctgg
     7981 cgaatgattt tgaattgctt ccggtattca aggcactttt ctgctctgag catttctttg
     8041 acgctacaga attcaatacc catatcaaaa gccacattga atattttaca ggcataataa
     8101 agcagtttaa ttacactttg aattatgagg agggcaatgc catgatttgg tatagttcat
     8161 ccttaggcca aacttatttt gacccggtag atgttgccgg ttggaaagag gacaagtcat
     8221 ggattgactc aggtagcctt ctggtacgat ggctttattc agagtggatg tatggggggc
     8281 tgatatggca gaatcaggat tttgtagtca gttatctgaa aaatctaacc aataacagca
     8341 atgatgtgga gtttgtcgta aggacagttg tcgatcagtt ttttgtttca gggttggcgt
     8401 cccaggcaga ttatgatcgt ctgcttattg cattcaaagc cgacctgcct gagaattatt
     8461 acgagcaagg attatggaat cttgactggg aaaaggattt catttgttgg cagaccaatc
     8521 tgcttcttaa tgagatggcc cgtttgccgg acgtcacgct ttgttagatt aacctccata
     8581 tcatcaaaat taaataaaat gaataacaag gatatagaaa aaagaggctt aaagcttgaa
     8641 gatggtgtat tgcatgaggc agatcatgtt gaatattcca gacggagatt tctcaaatca
     8701 ttcggtcttg caggcggatt gggttttatg cttggaggat tgcccgtgag tgcatctact
     8761 ctgacgcgat ggtccatgcc accaattctg gagaagtcag atcgcatcct gattatgatt
     8821 cgtctgaaag gggggaatga cggcatcaat acaatcatac cattggatca gttatcgact
     8881 tatcacaatg caaggccatc aataggctat caggataatg ttatgataca gctcaatgat
     8941 cagatagcta tgagccctca cctggccggg ttgaaacgcc tttgggatga gggtatgatg
     9001 aaagtggtgc agggcgttgg ttatcctgag caaaatcttt cacatttcag gtcttcagat
     9061 atctggtcaa gtgcctcgga tagcaatgaa gttgtacgtt ccggttgggt gggacgatat
     9121 gtggaggaga cctttcctga ttatgtagag aatccgccgg tgtatccacc ggctattcag
     9181 atcggtggcg cgagttctat cctttttaca ggtgatcaca ttggaaagtt gggttttgaa
     9241 gccttttcag gcgcacagct ggaagatttt gccactacgg gcaatgctca ctcccttcag
     9301 gacttgccac cttgttccta tggcgatcag ctcaggtatg tcagaattat taccaacagt
     9361 acatacaagt ttgtggaagc aattaaaacc gcctacagct taggccctga gactactttg
     9421 acttatgagg atgagaggtt ccctgaatca ctcaagatta ttgcgcgctt gatcaaaggt
     9481 ggtttgaaaa cgaagattta tgtcgtcgag ctggatggtt tcgatacgca ttccaatcag
     9541 atcggtaagc atgaggaatt gttaaaacaa ctcgccgccg cagttgatac tttttacaaa
     9601 gacctggaag gtacagaata tgaggagaat gttctttcag taaccttttc tgagtttggc
     9661 cgaaggattg agcagaatga aagtcccggt accgatcacg gtgctgccag tgtattactg
     9721 gcttttggaa aatgtcttga aggtaatgga accctaggca aatatccaag tctgaccgaa
     9781 actgatgaag atggaaacct gatttttaac gtcgatttca ggaaagtcta caacactttg
     9841 tttacggaat ggctttgtct ggaagatgct gtttctgatc agattttact aaaggatttt
     9901 ggagatccgc tggatttcgg ctttacttgt caggaagttt catccgtgaa gacaccggcc
     9961 aaaccgggat ttgtccacag tgcctcctat ggcctgggtg atgtatttgt caattatact
    10021 attccaaaag atgtgatggt gatggtggaa ttggtatcta tgtcaggcca acccatcgtg
    10081 cttgaacgac ccatgatgaa aaaggccgga tcctacagta ttcctttgca acaagccagg
    10141 cgcaggctta tgccgggtgc ttatgtgtac aggatcagag ttacaggagc ggattacacg
    10201 ggtaagttga taatcaactg ataggattct catgaagtag gcgaatgttg taagcggcaa
    10261 gatgtagatt ggaaacattg aaattttgaa aactgtacct tgttgtctgc atctgtggat
    10321 atctcggttt attcgcaggt tctttattta ttgtttcatg atgacctgcc tgacttcagc
    10381 catggatcat tgcagggcat agcagatcgg gaatctaata ccgaaggttc attcgtttta
    10441 gaccaatttc attgatctat aagactgtaa cctcggcatt atactaaaaa tttaaggact
    10501 attatttaaa tcatcttggt ataactcgta caggtcatga tagttattgg cagaatagtc
    10561 gattgtatat tttttgcagt tgccttatta tcttagattg accctggctt tcaaactgtt
    10621 ttctctggct gaacccaggt cctgtaccca atatcctcct ttttttgcta ctcccatttg
    10681 tttgattccc ggattcataa tattgaaaca atgtcccggg cttgccatcc agtctgagac
    10741 cacctgggcg atattcttct gaccgtaggc aatattttcg gcgactatct cccaatcata
    10801 acccttttct ttgattctac caggatagtt gcggccgtcc ctgcttttat gatcaaagta
    10861 tccttgtcga tgcatatcga tcgcatgctc ggctgctgaa gcggccaatg cgtcattcca
    10921 tgtaagtgct gatacaggct tcattctgac attcccacaa ttgcatccat tcaatctata
    10981 ttgattgacc tcggcaagca tcgcagcctt gaattcttca taattcctgg gtgtaggtat
    11041 gcccaccaat ccaattacta tcattaacaa gggaatcact taatacatct attttttatt
    11101 aggactcctg aaaggcttga aatagtatgt gcaatatccc ttttaactaa aaattatgta
    11161 ttcagtaggg gcaatggctt tctggtccgt acagtgccat tttggaaacg gccttttatc
    11221 atgacgtcaa tgtataaacc tgaaaagaga ttgcattgca aagcactgta gtatattgcc
    11281 gataagatca gtgaatgcag aacgatcaaa tcaaccactg ataaatttta gagaaagaat
    11341 agtgccatat aattcaaaat tcatacatta tggactatag gccttgaaga taatatctat
    11401 ctgactgggt ggaaattaca cttcattctc aacacgccta ttcctcctgt aataatccct
    11461 gtatcccgcg agcgtcaaat caatactcaa cgccctcaaa tgtcaatccc tgagcaggca
    11521 ccgaaaaagc aaagggcaga acgacacctt cgagagatag ttccttcata cgctctaaac
    11581 ttagtttgcc aacggcaaca ttggcacaac cgcccacaat caggcggacc atgcctctca
    11641 ggaagcgatt agctgcaatg tgcagttcga gacggtcttc atgtgccttc cattcgcatt
    11701 gaatcatatc acagtaggtc gtcttattgt ttgagccggt tttggcaaag gatgaaaaat
    11761 ccttcagact tgcaataaat gtagccgcct cattcaggat atgcacagat tccggtgtaa
    11821 ggcggtcgag gtaccagctg tatggttgta tgaaagggtc cttggaggta tggattcgat
    11881 agatgtatcg acgggatttg gctgagaatc tcgcatggaa attatcctcc attaccttaa
    11941 tcgatcttat gacgatatcg tcgggcaaga ttctgttgat tttgaataag aatggagcat
    12001 cttccggtat ttcagggaga tcaaaatgaa acccatagtt gcgaccatgg accccggaat
    12061 ctgtcctgcc gcatcccaca atggcaatag gcgaacggaa atagatggac aatgcatgct
    12121 caatagcttc ctgaatgctt atcccatttg gttgcctctg ccatcctaca taatttgtac
    12181 cgacgaaatc acaaaaggcg aggaggcgca tcaggcaact ttaaggttgg cttcgatacg
    12241 ggatccggct aattttgtct ttacatagtc taaatccacc actaatcgat caacattttt
    12301 ctttgaggga agctcgtaca tatcatccat taatattgct tcgcagatgg accgcaatcc
    12361 tcttgctcca agtttataat ttagagccgt ttgcgctata taatcaagag catcatcagt
    12421 tactatcaat tctaccttct cgtattcaaa cagtttgata aattgtttga tgatggcatt
    12481 cctgggttta ataaggattg atttgagcat gtccagattg agtggatcaa ggtaggtcat
    12541 gataggcagg cgtcccaaca gttccggaat caatccaaaa tgcttcaggt cttgatgcat
    12601 tgcatattga tacaagttgg actgatctac cttttctttt gcctcatggc tgtaaccgat
    12661 gatctgagta ttgagtcttc tggcgatgat tttatccagt ccatcgaacg aaccaccaca
    12721 aataaacaat atattttggg tattgacctt gatcattttc tgttcaggat gctttctgcc
    12781 tccctgtggc ggtacgttga catcagtacc ttccagcatc ttcagtagtg attgttgaac
    12841 tccctctccg gaaacatccc tcgtcaggga aggattgtcg ctctttcggg ctatcttgtc
    12901 tatttcgtcg atatagacaa tgcctttttc agccattttt acatcatagt cacagacctg
    12961 gagtagtctg gtcaggatcg attctacgtc ttcaccaacg tatccagcct ctgtgaagac
    13021 agtagcatct acgatagcaa atggaacatt cagcatccgt gcaatgctct tggctagaag
    13081 tgtcttaccg gttccggtcg gacctacgaa gatgatattg gatttttcga tctcgacgtc
    13141 cttatcgtgc tggtataacc ttttgtagtg attatacacc gctacggcca gtgtcttctt
    13201 cgcttgttcc tgacctatca cataattgtc aagatattcc ttgatttcct taggcgtacg
    13261 tttggcgaag tcgcttacct gtagattgtc cgagggttgt ttgtcaaaga tttcctgctc
    13321 aatgatatca tatgcctttt ccacgcagga ttcgcatatg tgtccatcga gtccttcaat
    13381 caggataata gcttcatttt tatcctttcc acagaaagaa catctttgta aatccttgtt
    13441 ttttgccatt gctttacttt taatacaaaa tgaaccaggc gggcatcctg tatgtccggt
    13501 atatggttga gatttattta ttattaatct tgtgttagtc tggaggggtt ggtcttttcc
    13561 aacacttcat ccaccaatcc atattctttt gcttcagcgg atgtcatcca attatcacga
    13621 tcacagtctt tttcgataat gtcatagtcc tttccggtat gggctgcaag aatatcatat
    13681 aattcctttt gcattttctt gatcagctta taggatattt ccatgtcgga gacctgaccc
    13741 tccataccgc caagaggctg atggatcatc acacgactgt gatacaggca tgttcttttg
    13801 ccctttttac ctgcagccag caggacagcg cccatggatg cagctaatcc ggtacatatg
    13861 gttgccacat ccggagagac gtactgcatt gtgtcatata tgcccaggcc gttgatcaca
    13921 gaacctccag gactgttgat aaacatctgg atatcgcgct ttggatctgt gctctccaga
    13981 aatagcaact gagcctgtat gacattggct acatagtcat tgaccggcac gcccatgaat
    14041 atgatcctat ccatcatgag tcgtgaaaac acatccatgc tcacaacatt catttgccgc
    14101 tcttcaatga cggcaggcgt aaaaccatgg atattaggta ctgacacctg catatattta
    14161 tcaaggtgca tggagttcat attcaggtgt ttggtcgcgt attttttgaa ttcgtctttt
    14221 ggaatcattg actgttcttt gatttggtta attgatattg gataaataaa aagattgaaa
    14281 aaaagttcat tatacttatt gaaagcattc aatagcctgc caggagaaca ataggttgct
    14341 ttattattct gactgagtat aagatataag aatgtcctga attacttagg cttcctctcc
    14401 ggatgtatta tctgcatcct ttgcctcaac ctcttctacc tcgaaatcac cgtcatgctt
    14461 gtgctcatcg ttatgctcgt gtccatgatt gtggtcgtga tggtgatgct ccgcctgctt
    14521 ttcatatttt ttgttgagtt cttcgatctt ttcattgtat tcttcagtcg gtatattcac
    14581 cacctggatt ccatgttcat cgatcaggaa actagccagc ttggccatgt atatctcgtc
    14641 cttctttctc gctatgtgac gattatcctt ataggcgtct tcgaggaatt tttgataaat
    14701 attttcagga agatttgctc caccgaagta ttcattgatc tctcggatta gcgctgtatt
    14761 gacatctgat ctggatacct ggattccata ttccttgtcc ataatgtcct tgatccacgc
    14821 ccacttgaag tcctctttat attcatccag atgatactgc agggcatgat cgtcgagttg
    14881 tgacacctgg gtaaagaatt tcttataaaa gtcatcagtc agatcgagtt gagtttccgt
    14941 cagagccatt tctttaaatt catggaagaa gagcatattg cttgatattc tgtaataggc
    15001 atggtaatcc tcctttagaa gcctgatcag gtcttcttct gacttgatat ttccccgtgg
    15061 atccaaccgg gtaaagaact cctcatccat atcagcaaga actttttctt gtatgttgct
    15121 gatcgttacc ctaaattcat aatcaaatgt atcattctgt tcttcagaat ccaatttcaa
    15181 aatctggttt ctgagtgtcg cttcattgtc ggtaaagtct ttcagattaa tgtcgaatgt
    15241 ctcaccaatt tttttaccaa tgatgttctt gtttagatca tcggattgga tgttggaaac
    15301 agaataattg aaagtcattg tatggccacc ttctttgacc tcaccatttt ccagttgcac
    15361 tgcatcgaag ctgataagac tttcttctcc ggcaggttca ctactttccg ccatgccgcc
    15421 gatattgtac cgaatggact tgatctgccc atccagtcgt tcctgcgata tttccggcag
    15481 gtaataagtc aacttgccac tgtaatcgaa atctaaatct tccagactag cctcctttac
    15541 aaacacgagg ctggccgaaa agtcctctac cttgtcaatg ttgaacttat aatcttcatt
    15601 gacatcattg atgacaaagt tggaaaaagt attgatgtca ttgtctttca agtattcctg
    15661 agccttatca aacaagttct tattgatcac atcaacatac acctgtgtac catacatatt
    15721 ttttataaga tttacaggag ttttacccgg tcggaaacct tttatcttgg ctttttgtgc
    15781 agtttttttc aattcctttt ttaaaagatc ctgaatttca gcagcttcca tggtaacatc
    15841 cagctggagt tgattttttg ttatttctac cgggttgatg gtcattttag attaataatt
    15901 tgaatattga atgaatatag aaaatcaagg taaaaacgta ctgtatgagc gaaacaggcg
    15961 gcttttactg cttcttttcc aattttgagg tgcaaaggta cgattattct ataaataaat
    16021 tgattattaa cgcacgctaa attaatttag cagggtatca aatacataac attgattaca
    16081 tttgcgccat gaaactgatc agaaattttt gcgtaattgc acacattgat cacggtaaga
    16141 gcaccttgtc tgaccgtttg ctggaatata cccagaccat tgcggaaaga gacatgcagg
    16201 atcaggctct ggatgatatg gacctggagc gcgaacgtgg catcaccatc aagagtcatg
    16261 ctatccagat gtactatcac catgatgacg gccaggtcta tacttacaat atgatagaca
    16321 cccccggaca tgtggatttc agctatgaag tgtcacgatc catcgccgct tgcgaaggtg
    16381 cgctcctcct ggtagatgca agccagggta ttcaggcaca gactatctcc aatcttttca
    16441 tggcgatcga ccacgatctg gagattattc ctgttctgaa taaggtcgat atggaaagcg
    16501 ctatggtgga agaagtctct gatcaaatta ttgacctgat cggatgtaaa aaagaagaaa
    16561 ttattcttgc cagcggaaag acaggcattg gggtgcatga tattctaaga gctattgtgg
    16621 ataggattcc ggcacctaag ggagatcagg atgcaccgct acaggcgctg attttcgatt
    16681 cggtattcaa ctcattccgg ggtgtgatcg tttatttcag aattatcaac ggcatcatca
    16741 ataaaaatga caaggttcgt ttctttcaca caggtaagga atattttgct gatgaagtgg
    16801 gtatccttca attaaatatg gttgaaaaac cccaactgac tgctggaaat gtgggttacc
    16861 tgatcacagg aatcaaagtt tcaaaggaga ttaaggttgg tgatacggtc attttggcta
    16921 aataccctgc cactgaagaa atcaaaggtt ttgaggaggt aaagccgatg gtctttgccg
    16981 gaatttatcc gatcgataat gatgattttg aggaccttcg cgatagtctt gaaaagctac
    17041 aactcaatga tgcctcattg actttcgaac ccgaaagttc tgttgccctt ggattcggct
    17101 tccgctgcgg cttcctggga atgcttcacc ttgatatcat acaggagcgt ttgtcccgtg
    17161 agttcaacca ggaggtaatt actacggtgc ctaacgtaat gtatcatgca tttctcaata
    17221 aagcgcccaa cgagaggatt attattaaca cacccaatga ccttccggac cctaccttgc
    17281 ttcatcatgt ggaagaacca tatatccatg cccaggtaat caccaagccg gagtatatcg
    17341 gttccatcat gtccttgtgc atggacaagc ggggcatcct gaccaagcag acctatctca
    17401 ccacaagcag agtagagttg acctttgaga tgccgcttgc cgagattgtt tttgactttt
    17461 atgataaatt gaagtccatt tctcgtggct atgcatcttt tgattatcag cctttggatt
    17521 acaggaagtc ggagctcgtt cggctcgata tcaagttgaa tggcgataat gtagatgcct
    17581 tgtctgctct cgtgcataag accaaagcag aggaattggg caggaaaatt tgtgccaaac
    17641 tgaaagatct gctacctcgt cagcaattca tgattgctat tcaggcagca ataggtgcta
    17701 agattattgc ccgggaaacc ttatcagcca tgcgcaagga tgtgacagca aagtgttatg
    17761 gtggagatat ttcccgtaaa aggaaattgt tggaaaagca gaaggaagga aagaaaaaga
    17821 tgcgtcaaat aggtagtgtt gaggtgcctc aaaaagcatt tttggaagta ttgaaattaa
    17881 atgattaaat agtatagaat gaaatatttt ggaattttaa cttgtatcgc cttaatgagt
    17941 ttggcggctt gtaaaagcga atctgcagga aatgcagcaa aagccgatga taaggaagcc
    18001 tatgttgcat atgatggaca gaccaggcag atcgcttcag tggacactct ggtcaacaat
    18061 aagggagttc agtttgaact tacattcagg gggggatcaa ccgatggagc tgcccaaatc
    18121 aaaatcagga aggacggaaa gttaattgaa aatacggacc gtaaagcaga tggccgggtc
    18181 acggcagcca tgatcgaaga tattaatggc gatggtgagg gtgattttat tttctatacc
    18241 aacacagaag acaatacaag aataggtaac ttgtatggtg tggttagcga taatggaaag
    18301 tatcggcaca tctttatctc tccatttatt aaagagcctt atgtaaccaa ttatttcgga
    18361 agggattctt tctatgttga gaatggaata atatataagt cttttcccag gtatgagttg
    18421 aataaattga gacagaaggt ggattccgga aaacgatgga tacttcagta caagtatacc
    18481 ggaaaggata caattgacgt atccgaagga attattcagt aaatattcag taattattat
    18541 ggaaagtgga ttcgtttaaa tgattgcgaa tccactttta ttttttttat ctcccccagc
    18601 caatccttac gcccgcctca aaggcagccg gttttaattt gctttgataa agactgttgt
    18661 ccgtcaatgg gaaataatgc ttatattgta ggataggcga aattttgaca tatttcccgg
    18721 caaaaaagtc cagcccgaga ccaagaccgg ccatggcaaa atgctcattg atttcaggaa
    18781 cgacattatc cggttcgttt acccaattcc ttagttcata accaacaacc ggttgcagga
    18841 agaagtgatt cttgaaaaaa tgttgcctgg agaatgtcgg acattcatca cactgatcaa
    18901 aatggaaggg atagattgtt accgggatat tcatgccaaa gcttctgatg gatttgaccc
    18961 ttcctacctc cagattgctg gcatcaaata tatcaatgcc tttgatccga accatttttg
    19021 caaaaatttc cggcccgaat aaaaccccgt agttgactaa cttgattctg taatccagag
    19081 caacctgact cccttgctgg gacagcctgt cattggcctt gccgggaatg ttactcggga
    19141 tatcttcata atccaacaca tatttaaacg aaatgccaaa ttgagcgtga acatcggtca
    19201 acgagaagaa ataaaataat aaaattagag tggtagtgaa gtattgaatt ttgcgctgca
    19261 taaagggtcg tgaataaaat tttttaataa gcagtttcaa taactaaaag aatcctatgc
    19321 caaagcttac atcccttttt agtcgtatct ttgcattttc aaatgtacag tgattatctg
    19381 aaatgaattg aattttcatt tcaattttta tatagttatt atagttataa tataaattaa
    19441 tttgaaaacc agaacattag aacaaaataa agtaagcgta attacccttg gttgcagtaa
    19501 gaacctcgta gattccgaaa atctgattac tcagctcaag ggtaatgact tcgatgtaca
    19561 gcatgatgag gtgaccaggg atacagatat tgtcattata aatacctgtg gattcataga
    19621 tcttgctaag gaagaatcca tcaataccat cctgaaatac gcgaaggtca aatcaaaagg
    19681 tggcatagac aaattgtatg ttacaggctg cctgtcacaa cgatacaagg ataatctgga
    19741 agaagaaatt cccgaggtgg atgcttattt tgggacattg gaattgccac gtctactcaa
    19801 taccttagag gctgattata agcatgaatt ggtgggtgag cgggtcttga ccactccttc
    19861 tcactatgcc tatatgaaga tttcagaagg gtgtaacagg acttgtgctt tctgcgccat
    19921 cccactcatg cgcggaggtc atgtatccaa gccggttgaa gaacttgtga aagaggctaa
    19981 aagcctggcc cgtatcggtg taaaggaaat tattctgatt gcgcaggaat tgacttatta
    20041 tggtctggat atatacaaaa agagagccct gccggatttg cttgatgccc ttgccgaagt
    20101 agaaggtatc gaatggatca ggctacaata tgcttaccct agtaaattcc ctatgggtat
    20161 aatcgatgcc atgaagcgac acgataaggt atgcaactat cttgatctgc ctttgcagca
    20221 tgcttctgac aagatattgc agtcgatgag acgccagatt acacgggatg agacaaagca
    20281 gttgatcagg gatatccgtc aaatgatacc gggaatagca gtcagaacga ctttcatcgt
    20341 cggcttcccg ggagaaaccg aagaagattt tgatcagttg tgcgattttg tgagagaaat
    20401 gcgttttgaa cgccttggtg ttttccagta ttcccacgaa gaggacacag ctgcatacga
    20461 gatggaggat gatgtcccgg aaagcataaa ggcagaacgt gccaatgcac tgatggctat
    20521 tcaacaggaa atcagcctcg aacttaacca gcaaaaaatt ggtaagatat ttaaagtact
    20581 tatcgataaa aaggaaggtg gatacttcta tggcagaacc gagttcgatt ccgtagaggt
    20641 tgacaatgaa gtcctcatag acgcttcttc caactattgc tctgtaggcg ggttttgtga
    20701 cgtcatcatc gatgatgcca ctgaatacga tctgataggc catgtgattc aacccggtta
    20761 aatttatttt cgatatgaat aagctatata attatttgtt tgtgcgacga caccctcttg
    20821 tatgggcact cgccattatt ttgatatttt cgattctgat ggtcgtcaat tattactgga
    20881 ttttcccaaa taatccggaa tggatgtttg ttgcagcctt cattggcgtt cttatgtatg
    20941 gtgtctatag cgcttttatg ctctttataa ccaagaaaat catgctggac tggaatcagt
    21001 cattcatcgg tttcttcctg gtcgctgcca ctttgtttta tgaaggtatg ctgatcagtg
    21061 ataagagctg ggatgatctt tccaactata cgccaatcct caaggtaatt ttaggggtgg
    21121 ccctcatcat catgagcatg tccatcatgg ccagaaaggt gctggaaaaa atgaaacaac
    21181 tggatcgtta acctgtaacc ttaagaatga atctgagtaa gcggattagc ctcatgacct
    21241 ctttgcggga ggatattgag ctaaataaag attattgggc tgctaaaatc cgggaagcag
    21301 agatgatgaa tccctggttt actccatcca gta
//
LOCUS       DAOQMC010000007        20552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_904_length_20552_cov_5.114163, whole genome shotgun sequence.
ACCESSION   DAOQMC010000007 DAOQMC010000000
VERSION     DAOQMC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20552
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_20552_cov_5.114163"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PKV31_00670"
     CDS             <1..1101
                     /locus_tag="PKV31_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91388.1"
                     /translation="SLITASYGRMFSYDNQANTWTALKVPYEGDQTITLTKLGLPVMD
                     QDTIFAFAELLANNSGPKFSLIYSTDNGNTWQGDNNAQPFGYTIPEILKINNRIFGSS
                     GDVFATTNNGGITWKNITPEDPLSLDAHNWNGYNGYIYGITGANRIIRFDNNYKIPKW
                     KEVDSDIPADKKILAIANIDEALFAMAPGEIYVSTDDAASWSRVDVTLPFERIRNWTY
                     YKNILFVTCDQGIFYTKDRGNTWTSLNDGLESPDLTVVKIAFDHIFVVNNAVTVLKRS
                     ISELLSTSENLAENQMMLYPNPADLYFTIPFIPGESNLQYRISDMKGAVVRSGKTADG
                     GQVNVNNLTAGMYIVTVMSEKGNLTGKLIIQR"
     gene            complement(1477..2949)
                     /locus_tag="PKV31_00675"
     CDS             complement(1477..2949)
                     /locus_tag="PKV31_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91389.1"
                     /translation="MKAINSNRFYTRIFIFIMFAQGVAAQDYSPVRENLPPYIFETSK
                     EYDESASMFMRGTMDSVINNGPVRDFYPYNTLFFGDNGNLSCLYMLHHSWLGARISVD
                     DSEDAATFLYQNVSNNSFPLITYKLRLEYSGNPGSQWMADNDFSILATVVSVEQTSWK
                     GIIDDSIKIIRLQYSGSNAQIKWSSGHIVISKHHGILSFPDLRYFPFEWNTIELAGGP
                     GFPVFDPSARDMYYMKAGQSYIAASKYGFGVKYPKYKEVRCLEILSDVQNTVTRRVQI
                     INLTYSYTDQKWKSDTSVKVEVIDFDSIIINQPVDRFFKLKGVDNIYGHIIMNKHRQK
                     YYYLSAAPGANNCVPVYTDQCAQYGFDGGFDTYWDCPMFVEFDYNQPVFIDYGDHTWG
                     HDINIDSLLTTRKISKLEGVTISPNPASSHFYMTIPPTVNLSKVFMTDILGNRVAIKV
                     EKMTAEKMELNTDVPGGVYLITGVDEAGNTYFLGKVVIYR"
     gene            complement(3160..4014)
                     /locus_tag="PKV31_00680"
     CDS             complement(3160..4014)
                     /locus_tag="PKV31_00680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB91390.1"
                     /translation="MKFTIITPAYNMSSTLGRAIESVLAQTYPDREMIVIENIRPGEK
                     RLENTYEDKPGIRILSGTFGDRSEARNFGIAHSDGDYITFLDADDALDPGYLEHFSEI
                     FRQDPDCIAISDILKIEEGKGRHWQKAGLYQGNPLKYAVNEGNITFAARRGYFLEQPF
                     EYDYWEDKLWLGRMGEKHPFKFTGKPDYQYYITSKIFSESDFHHSMNIETEAINALYA
                     GLSAPYALKYPKTSALNDFYIKKAYVALSLGLKEIANDLITQRIKKGLSIKTFLRWSK
                     LKWEYLTQ"
     gene            complement(4624..5694)
                     /gene="serC"
                     /locus_tag="PKV31_00685"
     CDS             complement(4624..5694)
                     /gene="serC"
                     /locus_tag="PKV31_00685"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNB91391.1"
                     /translation="MKIHNFSAGPSIMPQEVFEQASQAALNFNDNGLSLLEMSHRSKP
                     FQEVMDETHYLVRELFNLPDDYQVTFVTGGASTQFFQVPMNLLNDNEKAVYIDTGVWA
                     NKAAKEAKNFGEIDVAASSKDKNYSYIPKDYTIDPKARYIHLTSNNTIYGTQQHWWPE
                     TDLPIVCDMSSDIFSREVPIERFGLIYAGAQKNMGAAGVTLVIVRKDMLSHVSRVMPT
                     MLDYRTHIDNGSMYNTPPVFPIYISMLVMRWIKKMGGVKGMEVINEAKAKILYDEIDR
                     NPLFKGTTAMEDRSRMNVCFVMEDPTKEEAFMNLTKANGISGIKGHRSVGGFRASIYN
                     AMPQSSIQVLVDLMQEFERTNG"
     gene            complement(6051..7724)
                     /locus_tag="PKV31_00690"
     CDS             complement(6051..7724)
                     /locus_tag="PKV31_00690"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNB91392.1"
                     /translation="MENDLSIAQKAELTHIRNIADQLNIKEDDLIYYGRYKAKIPLNY
                     INEEKVQKSNLILVSAMSPTPAGEGKTTCSIGIAQAMNRIGRKTTVVLREPSLGPVFG
                     IKGGAAGGGYSQVLPMEDINMHFTGDFAAIEKANNLLAAVIDNSIQNKKNTLNIDPRT
                     VAWKRVMDMNDRSLRSIVSGLGGKGNGVPREAGFNITAASEIMAILCLVNNRRELKEK
                     LGNIYVGNTYDGKPVFARDLKAHGAMAALLKDAIMPNLVQTIEQTPAIIHGGPFANIA
                     QGTNTIIATKMGLSLSDNVVTEAGFGFDLGGEKFLDIKCRMGNLVPRCIVIIATIRAL
                     KYHGGVDLADLKAENVDAVRAGFVNLEKHLNNSRRFGIPAIVAINRFVTDTDAEVNAC
                     IEKCKSIGFDAHSIEIWAKGGGGALNIAEAIGKIVDNNQQQQFRPLYSTDTSIEDKIS
                     AIAKNIYGASSVEFGAKAKADLKVIKRLKLTHLCVCIAKTQKSFSDNPDRLGAPEGFV
                     LNVREIEIAAGAGFVVPVTGEMMRMPGLPEVPSAEFIDIDDDGNITGLF"
     gene            complement(7865..11425)
                     /locus_tag="PKV31_00695"
     CDS             complement(7865..11425)
                     /locus_tag="PKV31_00695"
                     /inference="COORDINATES: protein motif:HMM:NF039901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HNB91393.1"
                     /translation="MHRIGQITASVKVNDLALGYELNHLLADLLDSDLDGELSALLDE
                     YSTNSLIKLFEQVSIKIEIDEQENHRLRIKEEILRQLREILMKDVYDFEYHLSSDRKG
                     EFNIANPNISGSGKVISEVSLLKQYLTRFFQSGSSLELARFRSDELRRQIQQLIDTPS
                     FEFIEYINTLVRQDERATLRIANFFTTHEIEAFLVEIAGRYSFSIVTINQLKDMLLNL
                     TGTDDELGKKLILLSIMAYLKETSEKQLDSEIKHVRKRILSFQISSDTKKSDLIGADK
                     QAGFKSTILQELILFEAYDFSAKVLQNTGKQLKILKEHFPQRDYNLKTDDFPVIQGII
                     ESRQTQPTVQRSAWSYDIRHYLFSSFEFIVQHGTFPFWLRDDESQKLFLQLLRNILDS
                     DVSSFKEKVKNLLQGIHDPMKIYSNIVSGFPSEVRTSLVNTLSENLPDTLQLFRHYYL
                     NFKKRIIEERHVFSAAVKHFFEKDFSEFKMPIIDFMLAGFLAKKTFRTIKEIFSVFLI
                     IHTEEPDGLTGPMEDMNYLFGQDFKVPDGEGEIKSIMDQGRVKSKNVPDNDLIDSTPE
                     RFTKGQNLNFELKIFEDFTENIQEQKAETYMEEYIQWMVDVASIDDYKLPDDIRLFVL
                     NSGMQEMEKRKLAMAEFLHLIFTQDSWLISNKEFLNTAETFYREITSILGDIKDDIGY
                     LKTLQAKIDQHPSDFKEFFVRANNYLGTSLSGFALWKDYIKQAKPSLLFSEDSVNDIR
                     SFLALVSHLDQSDLLSFVSILDHEARGMYARIFMPDAHRPLSFPDKNKLPDQIIFDQI
                     VHSSAGLHFSIDDETIQKAKQFFTKIFRLHRDLKYLRELEGITKDTQPRVVILNILEE
                     RQVADKSLKEIAETSGETTHLFDEALFVQLRSKYGELIAKIGPTELVKRLDTHLSTGE
                     LLKLSDTFISVSDIEFNLKRYVIYVGEKYGVPLHYVALALLDKLLNLGSSQFIFPQYK
                     EVLYMMSFHLYDLALKLINAPKFISKDKEEEKVYNMPAWNLESDENMSIRVTNAGLVI
                     CNPFIVLLFQRLGLLTPEKKIKDNESINKAVIVLNYLSYGDIGIKEFNLGLNNVLCNV
                     PRNFYYDFSVELTPEEKELCDSLLENICNQWTALKNTSPDGLRHNFFIREGSLSYDGT
                     SYKLVVAEKAYDILISKVPWTIGTIKFPWMESFLFTDWKY"
     gene            complement(11427..13715)
                     /locus_tag="PKV31_00700"
     CDS             complement(11427..13715)
                     /locus_tag="PKV31_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91394.1"
                     /translation="MPPVLTIKKFPEYSEATDYAGLVRDYLDACQRYSGKVWTDYNVH
                     DPGVTSLELICLAITELGLKSETDIFRIIFNSFKMDFDADDYALYGPLQVFPCTPLTY
                     EDYRKLLVDKFIDVVDNIWIQPNIFTKEGASYKGLLNVLVKPKPGVEKDALSERIYNY
                     FKSIKNIGEDIYKVIILNPVKITINLVASIKGNILPEEVQARLRMLIFNEVERPITFD
                     SYLHALQNGEEINEVLSGPLLENGRIQDASIRNSDLDAQNKIYKSKILHLIAASEFID
                     RVISMDLFVNGVAIDSEGIELTETEFPSFDFYESGFELYKDDNLLYINKETQEYIYKS
                     NYRTKKVFSKKSLVEYLLPNIKSNISLKEITEFYSIQNQFPKIYGISSYGQVQQTEKA
                     RLSRVRFKAYLAMFEQIMQNYLSQLANIDKLFSLKRYGDYTYFYDYPEGIPDFQEVTF
                     DKKEQFIAKLGTIINKYDDRFPRRNMFIDHLCARFGEDLSGNLIAGEYIESTYDLNFK
                     YLKEIKLKSTFLRNYVDLGMYRTKSRMGDSKDNTGLSSLQTRLFILLGIENFQKRIVT
                     KFPDFKEEKTKNKLKVEIKSNEDLKSFFKDAVDLNNYSIEEKKNKYLLKFSMDNAREY
                     KIVESTAYVECENTMEDIRNHSIALNEHCEYFYLVENILLRPEVESLATSDPYSNICS
                     IVVPNWPYRFQSSYFKNNFKNLVLANAPIHIKIDLFFIDYSQMAHFEKLYYQIEEANE
                     KRAFCENEKKALTEFLLSLNAY"
     gene            complement(13737..13904)
                     /locus_tag="PKV31_00705"
     CDS             complement(13737..13904)
                     /locus_tag="PKV31_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91395.1"
                     /translation="MNKYRLILNAEEVKKILDILFERPFKEVYELIGRINEQLVEQEE
                     YNAESDDEEED"
     gene            complement(13919..14821)
                     /locus_tag="PKV31_00710"
     CDS             complement(13919..14821)
                     /locus_tag="PKV31_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91396.1"
                     /translation="MERTAQQVYRENLIKRFTDPDLQDFVQSTINILSDGIDIDDING
                     LKLESKGNARRMMSFYDKIKSKSSLWDINISQTQAKNNALNIAAQGKNILTVVDNGSV
                     GICKDDPAFQLDVDGLVGSTGRVGNFAIGKVPADGEWHILINDMSGTVGFEAFAHIIN
                     DKEDRYGLTYGVLLMSEEKGFKNRIRSVEASSKWLWGKWFNKIVFRWAFDTSNTSVTE
                     RRYRLEMKSRTRQGLPGQETPYIYYRLAKIWDKQYETGIDLNLTNVHSTTYGRAESVP
                     DEPIRNIPQESVSQPKRSISIKTK"
     gene            complement(14824..18735)
                     /locus_tag="PKV31_00715"
     CDS             complement(14824..18735)
                     /locus_tag="PKV31_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91397.1"
                     /translation="MIRDPKYIGTTQSTRLENRTNPLEIAIDDRPIENLLSILYEYAS
                     LLHFKESNSDDSKDWQAFFNYDLTSILALSITKDLAQAQFDFNLKVKEFSRATDGNIQ
                     IEALEAMISVVNSLLSEFNNLRDRLLKIQYPDGEIEKFVYFDIEAYYEQSVLDYIYEL
                     HAIILGAIPHAEKQKSAFNLGYQIIESSPEDVSQEDIPNKLESVFIRLRILFNNLLET
                     SLAIRDLLIPYFVRSLSQKDDHQPHIGLLLGFLEQYKKLQTSINKVPENYLHFYYIDF
                     LKQKKRKHIAPYAYVSFAISPYVENKTIPENTILLASLDKDGLPHRFSTKYPVNVGRY
                     TLSDIKTLYFDRGRRETLTNKYSIITESFMAPYANSQDGKGSPFNSDKLPAWPTFGEG
                     QIEKEPSLRNMIESELGFSVASSVLMLGEGKRSIKIRLNFTEESVTEIVRLFKDIASK
                     EHKKSTMLEVFYKIFSTKSKKRNITILLTSAKGWHKVDPQTILIFQDGEEEWDPRHIC
                     ISFNLSPMAPPIIPFNEHLHSGKYISGSQPILMLLLNNDREPYLYSFLQKLVIETIEI
                     DARVENVRNLSVYNEYGLIDNSQPFYPFGPTPVKNSYFLLGNREIFSKKLDEFELNID
                     WFDLPETAGQFNQKYEVYERRYDEFKVQIGNLNDGQFVMEDNSRPLFMTKEIIGSDKL
                     SDEGLFTKYTLDSGDLKSLGFNSSESMEIADQFSTNANVGFLKFVLDEPEDGFGQQKY
                     QEILAKQMLKNARLINKAFVEDENEDSPELGFPEMPLIPMVSNIHINYKASDVIELTQ
                     DAVNFQEINHIHPFGYEKIYPNIKYRDRLPYLLPQYEEDGYLLLGLKNCKKAEIISLL
                     FQMTPVYYQDRDQYILPIIRWQYLTYNNNWKLLQGENILRDETDSFTKSGVIQIKIPS
                     DISTMSQIMEPNKFWLRVSATGDIQLLNHTLAIYPNATLTRWVDQSDPSGLNTPFPPE
                     SIIALDNKIHGVTSMIQPYESFGGKAEESNIEFYTRISERLRHKNRGWTAWDIERLVL
                     NQFDDIEQVKCISGVTESNAQRYEYLDLHDDGGSFEVSYVNEMEDFISSKVIVVIVPK
                     KLKYLKEHLTIANFRKLEQVKSFLKDKMSPFVTLKVRNPEFEYIRISCDIKFVDTGNE
                     GLYIARLKREITKFICPWYVDESDALEFNKELQVQALKDYIKSLDFVVFITRFSVIQI
                     IENGFQYHIVDSAASQDLTQILKPYRPWSTFIPDQDHLINIVEEEIEVEPTSLQPPVK
                     FKNEYNILSKARILKLKSIGGQKRRTVKENNLPFNPFTLEI"
     gene            complement(18753..19208)
                     /locus_tag="PKV31_00720"
     CDS             complement(18753..19208)
                     /locus_tag="PKV31_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNB91398.1"
                     /translation="MDIDQINIKKIDGVRSFLGTGWAFPPDFNINTGETLVVSDFQDI
                     SESLRILFSVTPGERVTHLKYGCDLYAKIFEPIDSTFEFTVKEMITTAINDYEPRVEV
                     LDILTDIKDYADGLVKIEIIYRVRATNTRHNIVFPFYKNEGTSLPEEIS"
     gene            complement(19220..>20552)
                     /locus_tag="PKV31_00725"
     CDS             complement(19220..>20552)
                     /locus_tag="PKV31_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNB91399.1"
                     /translation="SQIIQYGCCDWDFILSRLEQNGLILLTDDDKVKVKKPDFNSAPV
                     HLLTHGVEIIKSELEIDGSFQLKKVKTISWNPDNQELNESVNRDVSINLQGNSGQIEF
                     SVLADKFNKKDFLLQSTGHLPEKDLEEWATAQFQRAKMSRTLGSITCNGTEKFLPDKI
                     VELKGLGKWFDGKAYIREVNHVLQSGSWITELIIGWQKDWYLESRTDIINPATSGQLP
                     AMEGIFIGKVKQLEGDPEGGYRIKLDIPVITGSSDGVWARLAQLHASDSHGAYYVPEI
                     DDEVVVGFLNADSRYPVILGSLYSSKLKSPYEHEDINKIKGFVSRSELKLTYHEEDKS
                     ITLETPGGRKFTMDDTAQHIKMEDGFNNSITMDASGITIKTTGRVEIDATMGVQMTGS
                     AMVKMDSSGPISVSGTLVNINGNVSAMVSAPTATLSGQMIATVTGAIVIIG"
BASE COUNT         5612 a         4298 c         4141 g         6501 t
ORIGIN      
        1 agcctgatta cggcttccta tggtagaatg ttcagctatg acaatcaggc caatacctgg
       61 acagccctga aagtgccgta tgaaggtgat cagacgatta ctttgactaa actgggcctt
      121 ccggtaatgg atcaggatac catctttgcc tttgctgaat tgctggctaa taatagtgga
      181 ccaaaattct cactgatata ctccaccgac aatggaaata catggcaagg cgacaacaat
      241 gcgcaacctt ttggttatac catacctgaa atcctgaaaa taaataatag aatcttcggt
      301 tcaagtggtg atgtatttgc aaccacaaac aatggcggca taacatggaa aaacattaca
      361 cctgaggatc cgctcagctt ggatgcccat aactggaacg gctacaacgg ctacatttac
      421 ggcatcaccg gagcgaacag aattatccgg tttgacaata attataaaat tcccaaatgg
      481 aaggaggtgg actccgacat cccggctgat aagaaaattc ttgctatagc caacatcgat
      541 gaagctttgt ttgcaatggc tccgggcgaa atctatgtca gcacggatga cgctgccagc
      601 tggtctcgtg tagatgtaac acttccgttt gaaaggatca gaaactggac gtattataaa
      661 aatatactat ttgttacctg cgatcagggc attttctata caaaggaccg tggcaatacc
      721 tggacctctc tcaatgatgg tctggaatca cccgatctga ctgttgtcaa aatagccttt
      781 gatcatattt ttgtcgtcaa caatgcagtt accgtgctga aaagatcgat ttctgagttg
      841 ctttcaacct ccgaaaattt agcagaaaat caaatgatgc tttatcccaa cccggctgat
      901 ctatacttta ctattccatt tattcccgga gaaagcaatt tacaataccg gatcagtgac
      961 atgaagggag ctgtcgtgcg ctccggcaaa accgctgatg gtggtcaggt aaatgtgaat
     1021 aaccttactg caggtatgta tatcgtgacg gttatgtcag aaaagggtaa tttgacagga
     1081 aagttgatta ttcagagata gcttctcatg tagcgtcaag tcgattctcc cggatcattt
     1141 gtcagaatgg tggtcgaata caatattcgg gtattttcag acaaccataa ctatgaagtt
     1201 tctatcgatt aaccggaatt tgtgtacagg atgaaaaaca ttttgatctt tcgatatggg
     1261 agtcgatcat aatacaactt ggtttttagg cgataaaaca tcatccgata acggctaagt
     1321 gctgcctaat cacgataatc caagcaaatc gggcaaaaga gacgaaaaaa aacaagggta
     1381 aattttacct ctattgtcag aacgagtgta aatatttcga atttttaccc tcgtattaga
     1441 gatgaatgta gcatttacat ttgaatgaaa taacctttat ctatatataa ctactttccc
     1501 taagaaatat gtatttccgg cttcatccac gcctgtaatc aggtaaacac cgcctggaac
     1561 atccgtattc agctccattt tctcagcagt catcttttcg acttttatgg caactctgtt
     1621 tccaaggatg tcagtcataa aaactttact caggttgacc gttggaggga tggtcatgta
     1681 aaaatgactg gaggccggat tggggctgat ggtcacacct tccagtttac ttatctttcg
     1741 ggtagtcagc aggctgtcga tgttgatgtc atgaccccag gtatggtcgc cataatcgat
     1801 aaacaccggc tgattataat caaactcgac aaacatcgga caatcccaat aagtgtcaaa
     1861 accgccgtca aatccatatt gagcacattg gtcggtataa acaggcacac aattatttgc
     1921 tccgggtgca gcgctaagat aataatattt ttgtcgatgc ttattcatta taatatgccc
     1981 ataaatatta tcaacgccct ttaatttgaa aaaccggtca accggctgat tgatgatgat
     2041 ggagtcaaag tcaataactt cgaccttaac cgaggtgtcg ctcttccatt tttgatctgt
     2101 atacgaatat gttaaattaa ttatctgaac ccttcgtgta actgtatttt gcacatctga
     2161 gagtatttcc aggcacctga cctccttgta tttaggatac ttaacaccaa agccgtattt
     2221 ggatgcggca atataactct gcccagcctt catatagtac atgtcccgcg cactcgggtc
     2281 aaagacagga aagccgggac cgcccgccag ctcgatggtg ttccactcaa aaggaaaata
     2341 acgtaggtca gggaaggaaa gaatgccgtg atgcttgctt atgacgatat gtcccgatga
     2401 ccatttaatc tgtgcattcg acccggagta ttgcagacga atgatcttga tactgtcgtc
     2461 aatgattccc ttccaggatg tctgttctac tgaaacgacg gtagccagga tgctaaaatc
     2521 attgtctgcc atccactgag agcccggatt gccggaatat tcgaggcgga gtttataggt
     2581 aatcaaggga aagctattat tggaaacatt ctgatacagg aaggtcgctg cgtcctcact
     2641 atcatctacc gatatacggg cccccagcca gctgtgatgc agcatgtata aacaacttaa
     2701 gttgccatta tccccaaaaa acaaggtgtt gtaaggataa aaatcccgga ccggaccatt
     2761 atttatgacg gagtccatgg ttcctctcat aaacatactt gcactttcat cgtattcttt
     2821 tgatgtttcg aagatgtaag gaggtaggtt ttctctcaca ggtgaataat cctgagctgc
     2881 cactccttgt gcaaacatga tgaaaatgaa tatccgggtg taaaatcgat tggaattaat
     2941 tgctttcata agccagtgat ttttacagaa aatgatggac ccgtgtaaat ttaataaaat
     3001 attttaataa tgacctggtt ttatcctatg agatgctgaa ttccagaggt ttcgacaaag
     3061 gaatcgaccc gaattgacaa gattgccacc acaatttttt aaaacaatga tatggaagaa
     3121 gtcagatcga atagggcttt ttggggattt attatttttt tactgtgtca gatattccca
     3181 cttcaacttg ctccacctca aaaaggtctt tattgatagt ccttttttaa tccgttgagt
     3241 aatcagatcg ttggctattt ccttaagtcc cagagacagg gcgacatagg cttttttgat
     3301 ataaaaatca ttcagggctg aagttttcgg atactttaag gcgtatggtg ctgaaaggcc
     3361 ggcatataag gcgttgatag cctctgtttc gatgttcatg gagtgatgga agtctgattc
     3421 cgaaaatatt ttggatgtta tataatattg gtaatccggc tttccggtaa atttaaacgg
     3481 atgcttttcg cccatcctcc cgagccacaa cttatcttcc cagtaatcgt attcaaaggg
     3541 ttgttccaga aaatatcctc ttcgggcggc aaaggtgatg ttgccttcat tgacggcata
     3601 tttcaatgga ttgccctggt ataatccggc tttctgccag tgtcgcccct tcccttcctc
     3661 tattttgaga atgtctgaga tcgcgatgca gtcgggatcc tgacggaata tctctgagaa
     3721 atgctccaga tatcccgggt ccagtgcatc atctgcatcc agaaaggtaa tgtagtcgcc
     3781 gtcactgtgc gctatgccga aattgcgggc ctcactgcgg tcgccaaagg tcccgcttaa
     3841 gattcggatt cccggtttat cttcatacgt attttctaat cttttctctc cggggcggat
     3901 attctctatt acaatcatct ctcggtcagg ataggtctgc gccaatacgg attcaatggc
     3961 acgacccagc gtggacgaca tattgtaagc gggagtgata atggtaaatt tcatacacaa
     4021 gattaaagaa acactatgta catgacgaaa aatattaagt ttaagtgaaa catctttagt
     4081 tttcagaatg aataacaaag attaaaaatt tcattaatag tttagtctta gtatgaaata
     4141 caaattgaat aatttataaa tcaggtatct gccggaagta tgttctgtgg aggatcggcc
     4201 ttgttgaatt tagcggatga gacggtgaat tgccaaaaaa tagcgaaggg atatacagtg
     4261 gagcgacagc ggagccttat ataccgagcg ttaagttttt tgagtgattc attgtcgaag
     4321 aagcgttaag aattcaacag agccttgatt ttttgttact tttttatcaa gaaaaaagta
     4381 aagagtaaaa tctcgttata caattggtga tacccaattc actttgacta tactaaaaaa
     4441 aacttgcccc tacacatcta aatagtagaa atgttcaatt tgatatgtac acctattctt
     4501 attataagat cttaaagtac cattttttca gatacatagc aaagaatatc tatcaacatg
     4561 acacgcatgc gatagcctaa atcttcagga ttatcaaata aaaatttaga aaaatccctt
     4621 taattatccg tttgtccttt caaattcctg catcagatcg accaatacct ggatgctcga
     4681 ttgcggcatg gcattgtaga tagaggctct gaatccgccg acagaacggt gacccttgat
     4741 gcccgagatg ccattggctt tggtgagatt catgaaggct tcttccttgg tcggatcttc
     4801 catgacaaag cacacattca ttcttgatct gtcttccatg gctgtcgttc ccttaaacaa
     4861 tggattgcga tctatttcgt cataaaggat ctttgccttg gcttcattga ttacttccat
     4921 acctttgaca ccgcccattt ttttgatcca cctcattacc aacatcgaaa tataaatggg
     4981 aaaaacggga ggtgtattat acatcgaacc attgtctatg tgggttctgt aatcaagcat
     5041 tgtcggcatc acccggctga catggctcaa catgtcttta cggacaatca ccaaggtgac
     5101 gcctgctgca cccatatttt tttgagcgcc ggcatagatc aacccaaatc tttcgatggg
     5161 cacttcccgg ctaaaaatat ccgaagacat atcacacaca atgggtaagt cggtttccgg
     5221 ccaccaatgt tgctgagttc catagatggt attattgctg gtcaggtgaa tatatcttgc
     5281 tttcggatca atcgtataat ccttgggtat ataggaataa ttcttgtcct tagaagatgc
     5341 tgcgacatca atttctccaa aattttttgc ctcctttgct gctttgtttg cccacacccc
     5401 tgtatcaata tatacggctt tctcattgtc gttgagcaag ttcataggta cttgaaaaaa
     5461 ttgggtactt gctccaccgg ttacaaaagt gacctgatag tcatccggta aattgaaaag
     5521 ttccctaacc aggtaatgag tttcatccat cacttcctga aacggcttgc ttcggtggga
     5581 catctcaaga aggctcagcc cgttgtcatt gaagttgaga gccgcctgtg atgcttgttc
     5641 aaatacctcc tgaggcatga tggacggacc ggcactaaaa ttatgtatct tcatggaaaa
     5701 acctgtttta aattcaaaat aaagtcacat tattatgaag tgacgtaaaa aacaaatata
     5761 aaataattgt aactattcca tagataaaat gctgcgcaat gtatataaat cactcttgcc
     5821 atcctggcga caatgaagtg gattgcaaag cggccctcaa ggaaagccaa tcgtttcttt
     5881 tatctctcta aagtcctttc gtatattctg taagtatttg cggttgaacc tattaattat
     5941 cgcatcgtaa gccgtttacg gtctgagtat gccattttca attgtttttc atatccttat
     6001 tgcgattgtg ctaacccggt tagttggcca caactttcat aaatccttca tcaaaacaat
     6061 ccggtaatat tcccatcatc atcgatgtcg ataaattcgg cgctgggcac ctcaggcaat
     6121 ccgggcatcc gcatcatttc acccgtgacc ggtactacaa agccggcacc tgctgcgatc
     6181 tcaatctccc gcacattgag aacaaatccc tccggggcac ccaatcgatc gggattgtcg
     6241 gaaaatgact tctgtgtctt ggcaatgcat acgcacagat gtgtcaactt aagtctcttg
     6301 attaccttca ggtcggcctt ggccttggcg ccaaattcga cggacgaggc accatatata
     6361 tttttagcga tggcagagat cttgtcctct atgcttgtat cggtggagta tagcggccgg
     6421 aactgttgtt gctgattatt atcaacaatc tttccgatgg cttctgcgat attcagtgcc
     6481 ccacctccac cttttgccca tatctcgatc gagtgtgcat cgaaaccaat ggatttgcat
     6541 ttttcgatgc aggcattgac ttctgcgtcg gtatctgtga caaagcggtt gatagccacg
     6601 atagccggga tgccaaaccg cctggaattg ttgagatgtt tttccagatt gacaaagcct
     6661 gcacgaactg catccacgtt ctcggccttg agatctgcca gatcgacacc tccgtgatac
     6721 ttgagggcgc ggatggtggc gataattact atgcagcgtg gcaccagatt gcccatacgg
     6781 catttgatat cgaggaattt ctctcctcca aggtcgaagc cgaagccggc ctccgtgacg
     6841 acattgtcag acagggatag gcccatcttt gtggctatga tcgtattggt tccctgagcg
     6901 atattggcaa atggtccacc gtggatgatg gcaggtgtct gttcgattgt ctgtacgaga
     6961 ttaggcatga tcgcgtcctt gagtagggcg gccatagcgc catgcgcctt cagatcacgt
     7021 gcaaatacgg gcttcccgtc ataggtgtta cctacgtata tgttgccaag tttttccttc
     7081 agttcccgtc ggttattgac caggcaaagg atagccatga tttccgatgc agcggtgatg
     7141 ttgaatcctg cctctcttgg cacgccgttg cccttgccac caagaccgga gacgatggat
     7201 ctcagcgagc ggtcattcat gtccatcaca cgtttccagg cgacggttcg cggatcgata
     7261 ttgagtgtat tctttttatt ctggatactg ttgtcgatga cggctgccag taagttgttt
     7321 gccttttcaa tggccgcaaa gtcgccggta aagtgcatgt tgatatcttc catcggcagt
     7381 acctgtgaat atccgccgcc tgcagctcct ccctttattc cgaagacagg tcccaatgaa
     7441 ggctctctca ggacgacagt tgttttgcga cctatcctgt tcatagcctg agcgataccg
     7501 atgctgcatg tggttttgcc ttcgcctgca ggtgtcggtg acatggcgga taccaggatc
     7561 agattgctct tctgtacctt ttcctcattg atgtagttaa gggggatctt ggctttatat
     7621 ctgccataat agataaggtc gtcctccttg atattcagtt ggtcggcaat gttacggatg
     7681 tgagtcaatt ccgctttctg tgcaattgaa agatcattct ccatagactt ttattttttt
     7741 aggcctaaat ataaatgaaa tattctcacg atgtaccatt caggctcctt caggcaaaca
     7801 ttaaaataat tctaaattgt gataagccta ctgtaaggca attgtctgga tatgctatgc
     7861 atgatcagta cttccaatct gtaaacagga agctttccat ccatggaaat ttgatcgtgc
     7921 caatggtcca tggaactttg gaaataagaa tatcgtaggc tttttctgcc acaaccagtt
     7981 tataggaagt gccatcatag ctgaggcttc cttctctgat gaagaaattg tgtctcaacc
     8041 catccgggct ggtgttttta agggcagtcc attggttaca gatgttttcc aaaagtgaat
     8101 cacacagttc cttttcctcc ggtgtcaact ctaccgaaaa atcataataa aaattcctgg
     8161 gaacattgca caggacatta ttcagcccga ggttaaattc cttgatgcct atgtcgccat
     8221 agctcaggta attcaatact atgactgcct tgtttatgga ttcgttatcc tttattttct
     8281 tttccggagt gaggagaccc agcctttgga aaagcagaac aatgaaagga ttacagataa
     8341 caagcccggc attggtaacc cggatactca tattttcatc ggattcgagg ttccatgcag
     8401 gcatattgta aactttttct tcttccttgt ctttggatat gaatttgggg gcgttgataa
     8461 gtttaagggc cagatcatac agatggaaac tcatcatgta taaaacttcc ttgtattgcg
     8521 ggaaaataaa ttgagaagac ccaagattga gtagcttatc gagcagcgcc agggctacat
     8581 aatggagtgg gacgccgtac ttctctccaa cgtatattac atacctcttc agattgaact
     8641 cgatgtcaga aaccgagata aaagtatcgg aaagtttcaa taattcgcct gttgacagat
     8701 gggtatccaa tctcttgacg agttctgtcg gcccgatttt ggcaatcaat tcaccgtatt
     8761 tggatcgtag ttggacaaat aatgcctcat caaacaaatg tgttgtttct ccggatgttt
     8821 cggcaatttc cttcaaactc ttatctgcaa cctgcctttc ctctaaaata ttaaggatca
     8881 ctactcgggg ctgagtatcc tttgtaattc cctccaactc cctcagatac tttaaatcgc
     8941 ggtgtagccg gaaaatttta gtaaaaaatt gttttgcctt ctgtattgtc tcatcatcta
     9001 ttgaaaaatg caagccggca cttgaatgga caatctgatc gaaaattatc tgatcaggca
     9061 atttattctt gtcagggaag gagaggggac gatgggcatc cggcatgaaa attcttgcat
     9121 acatgccacg tgcttcatga tctaagattg aaacaaagga gagcaaatca ctctgatcaa
     9181 ggtgcgaaac caaggcaaga aaagacctta tatcgttgac agaatcttca gaaaacaata
     9241 atgaaggttt cgcttgtttg atgtagtctt tccataaagc aaagccactc aggcttgttc
     9301 ctaaataatt attcgcccga acaaaaaact ccttgaaatc agaaggatgt tggtcaatct
     9361 ttgcctgtaa tgtctttaag taaccgatat catccttgat atctcccaat atggaggtga
     9421 tttcccgata gaatgtttct gcagtgttca gaaattcttt attactaata agccagctgt
     9481 cttgagtaaa aatcaaatgt aaaaactcag ccatagccaa cttccgtttt tccatttcct
     9541 gcataccact attcaaaaca aatagtctga tatcatccgg cagcttatag tcatcaatgg
     9601 aagctacatc caccatccat tgtatatatt cctccatgta tgtttcggcc ttctgttcct
     9661 gaatattttc agtaaaatct tcgaagatct tcagctcaaa atttagattc tgacccttgg
     9721 taaagcgttc gggagttgaa tcaattaagt cgttgtccgg tacattttta gacttcaccc
     9781 tgccttgatc cattatactt ttgatttcac cttctccgtc agggaccttg aagtcttgtc
     9841 cgaatagata attcatgtct tccattggtc cggttaatcc atccggctct tcagtatgaa
     9901 ttatcaggaa tacactaaaa atctccttaa tggtcctaaa tgttttcttt gctaaaaaac
     9961 cagccagcat gaagtcaata atcggcatct taaactcact gaaatccttt tcaaaaaagt
    10021 gctttacagc agcggaaaat acatgtcttt cttcaatgat gcgttttttg aaatttaaat
    10081 aataatgccg gaaaagttgc aatgtgtccg gcaggttttc agacaaagtg tttacaaggg
    10141 aagtcctgac ttcagaagga aagccggaaa ctatattgga atatatcttc atcgggtcgt
    10201 gtattccctg tagaagattc tttacctttt ccttgaacga actgacatca ctgtccaata
    10261 tatttcgcaa caattggaga aaaagcttct gactttcgtc atccctgagc caaaagggga
    10321 aggtcccatg ctgcacaatg aattcaaagg aagaaaacaa ataatgcctg atatcataac
    10381 tccatgctga tcgttggacc gttggctgag tttgtcggga ttctattata ccttgaatta
    10441 caggaaaatc atcggttttt agattgtagt ctctttgcgg gaagtgctct ttaagtatct
    10501 ttagttgctt gccggtattc tgtaatactt ttgcggaaaa atcataagct tcaaataata
    10561 tcaattcctg caatattgtc gatttaaatc ctgcttgttt gtcagctcca atcagatctg
    10621 attttttggt atcggaggaa atttggaagg agagtattcg ctttcttaca tgttttattt
    10681 ctgaatccag ttgtttttct gaggtttctt tgaggtatgc catgattgac aataagatca
    10741 acttttttcc cagctcatca tcagtcccgg tcaggttcaa tagcatatct tttagttgat
    10801 tgatcgtcac aatggaaaaa gaatatctgc ctgctatttc tactaaaaat gcttcaattt
    10861 catgtgttgt gaaaaaattg gcaatccgca atgtagctct ttcgtcctgt ctgacaagcg
    10921 tattgatgta ctcgataaat tcaaaggaag gggtgtcgat tagttgttgt atctgccggc
    10981 gcaactcgtc ggatctaaac cttgcaagct caagggaaga acctgattga aaaaaccggg
    11041 ttagatattg tttgaggagg cttacttctg aaatgacctt ccctgagccg gaaatattag
    11101 gatttgcaat attaaattct ccctttctgt cagaactgag gtgatattca aaatcatata
    11161 catccttcat gaggatttct cttaattgac gcaggatttc ctctttaatt cttagccgat
    11221 gattttcctg ttcatctatt tctatcttga tggatacctg ttcaaacaat ttaatcagac
    11281 tattagtgct gtattcatcc aacaatgcac tcagttcacc gtccaaatcg ctatccaaaa
    11341 ggtctgccag caaatgattc aactcataac cgagtgcaag atcattcacc ttgaccgaag
    11401 cggttatttg tcctatcctg tgcatgtcaa taagcgttca gagaaagtaa aaattcggta
    11461 agtgcctttt tttcattttc gcagaaggct cttttttcgt ttgcttcctc aatttgataa
    11521 tatagctttt cgaagtgtgc catctgagaa taatctatga agaatagatc tatttttata
    11581 tgtatcgggg cgtttgcaag gacgaggttt ttgaaattat ttttgaaata tgaactttga
    11641 aacctgtaag gccagttagg cacaacaata ctgcaaatat tggaataggg atcactggtg
    11701 gcaaggctct ctacctcagg tctaagcaat atattctcga ccagatagaa atattcacaa
    11761 tgttcattca gagcaatcga atggtttctg atgtcttcca tggtattttc acattcgaca
    11821 taggcagttg attcgactat tttatattct ctcgcattgt ccattgaaaa ttttagcaaa
    11881 tatttgtttt tcttttcttc aatgctataa ttatttaaat cgactgcatc tttaaagaaa
    11941 gacttcagat cttcattgga cttaatctcg acctttaatt tattttttgt tttttcttct
    12001 ttaaaatccg ggaatttagt tacgatgcgc ttttgaaaat tttcaatgcc cagcagaata
    12061 aacaaccttg tctgcaatga tgataatccg gtattgtctt ttgaatctcc catgcggctt
    12121 ttggttcggt acattcccaa atcgacataa ttacgcaaga aggtggactt taattttatt
    12181 tctttcaggt acttgaaatt caggtcatag gtggattcta tatattcgcc ggctatcaga
    12241 tttcccgaaa gatcctctcc aaatcgtgca cataagtgat cgataaacat attccgtctt
    12301 ggaaaacgat catcatattt gttgattatc gtacccagtt ttgcgatgaa ttgttctttt
    12361 ttatcaaagg ttacttcttg aaaatccgga attccttcag ggtagtcata aaaataagtg
    12421 taatcgccgt atcttttcag ggagaataat ttatctatat tggcaagttg tgaaagatag
    12481 ttctgcatga tctgttcaaa catggcaaga taagccttga atcgtacacg actcagcctg
    12541 gccttttctg tttgttgcac ctgcccataa gaggagatac cgtagatttt cggaaattgg
    12601 ttctgaatac tatagaattc tgtaatttct ttgagtgata tattggattt tatatttgga
    12661 agcaggtatt ccaccaggct tttcttgctg aacacctttt tggttctata attgcttttg
    12721 tatatatact cctgggtttc tttattgatg tatagaaggt tatcgtcttt gtatagttca
    12781 aatccggatt cataaaaatc aaaagaggga aattcggtct cggtgagttc aataccttcg
    12841 ctgtcgatcg caacaccatt gacaaacaaa tccatggaga tgactctgtc aataaattca
    12901 gaagccgcta tgaggtgcag aatctttgac ttgtaaattt tattctgtgc atcaaggtcc
    12961 gagttcctga tggaagcatc ttgtattctt ccgttttcca gcagtgggcc ggacaggact
    13021 tcattgattt cttctccatt ttgcagtgcg tgcaggtagc tgtcaaaggt gattggccgt
    13081 tccacttcat taaagataag cattctcaat ctggcttgaa cctcttccgg gagaatattt
    13141 cctttgatgg atgcgacaag attgatggtt atttttactg gattgaggat tattacttta
    13201 taaatgtctt cacctatgtt tttgatggat ttaaaataat tataaatcct ttctgacaat
    13261 gcgtcctttt caacacccgg cttaggcttg accaatacat tgagaaggcc tttgtatgaa
    13321 gcaccttcct ttgtaaatat gttgggttgt atccaaatat tgtccaccac atcgataaat
    13381 ttatcgacca gcagttttcg ataatcttca taggttaatg gagtgcatgg aaatacctgc
    13441 aatggcccgt acagggcgta gtcatctgca tcgaaatcca ttttgaaaga attgaagatg
    13501 atcctgaaaa tatcagtttc cgacttaagc cccagttccg tgatggcaag gcaaatcaac
    13561 tccaatgatg tcactccggg atcgtggaca ttgtagtccg tccatacctt gccggaatat
    13621 ctttgacatg catcaagata atccctgacc agtcctgcgt aatcagtagc ctcggaatac
    13681 tccggaaatt tctttatggt aagtacgggt ggcaaaatat actcatttag taattatcaa
    13741 tcttcctctt catcatcgct ttcggcattg tactcttctt gttccacgag ctgttcattt
    13801 atcctaccaa tcaattcgta tacctccttg aaaggtctct cgaaaaggat gtcaaggatt
    13861 tttttaacct cttccgcatt caatatcagc ctgtatttgt tcatcagatt ctatccgctt
    13921 attttgtctt gatactaatc gatctcttgg gttgtgatac cgactcctga ggaatatttc
    13981 ttatcggctc atccggcaca ctttctgctc ttccataggt tgtactgtgg acgttcgtca
    14041 ggttgagatc gatgccggtt tcgtattgtt tgtcccagat tttcgccaga cggtaataga
    14101 tatagggagt ttcctggccg ggaagacctt gccttgttct ggatttcatc tccaatctat
    14161 accttctttc agtaaccgaa gtattggagg tgtcaaaagc ccatcgaaag acaatcttgt
    14221 tgaaccattt gccccacagc cacttactgc tcgcttctac tgaacggata cgatttttga
    14281 aacccttttc ctccgacatg agcaagaccc cataagtaag gccgtaccga tcttccttgt
    14341 cattaatgat gtgagcaaag gcctcaaagc ctacagttcc tgacatgtca ttgatcagga
    14401 tatgccactc accatcggca ggcaccttcc ctatcgcaaa gttgccgact cttccggttg
    14461 agccaaccaa cccgtccaca tcgagctgaa atgccggatc atccttgcat atccccacgc
    14521 tgccattatc tacgacagta aggatatttt tgccttgggc agcaatgttc aatgcgttgt
    14581 ttttggcttg agtctgactg atgtttatat cccatagcga agatttactc ttgattttgt
    14641 catagaaact catcatcctt cgcgcgttgc ctttggactc aagtttaagt ccgttgatat
    14701 catcgatatc tataccatcg ctgagaatat tgattgtgga ctgaacaaag tcctgtaagt
    14761 ccggatcggt aaatcttttg atcaaatttt cacgataaac ctgttgagct gttctttcca
    14821 tagttatatt tctaaggtaa atggattaaa tggtagatta ttctctttaa ctgttcttct
    14881 cttctgaccg ccgatactct ttagtttcag gatcctggcc ttactgagaa tattatattc
    14941 atttttaaat tttacggggg gttgaagtga ggtgggttca acttctattt cttcttccac
    15001 gatgttgatc agatggtcct gatcgggaat aaaggtggac catggcctgt atggttttag
    15061 tatctgtgta agatcctgag atgccgcact atctacgata tggtactgaa aaccgttttc
    15121 aatgatttga ataaccgaga atctggtgat aaagacaaca aaatcgaggg atttgatata
    15181 atctttcagc gcctgtacct gcaattcctt attgaattcc aaagcatcgg attcgtcaac
    15241 ataccatggg cagataaatt tagttatttc gcgcttaagt ctggctatat ataaaccttc
    15301 attgccggtg tcgacaaact tgatatcgca tgaaattctt atatactcaa attcaggatt
    15361 gcgcactttc aaagtcacaa agggagacat tttgtcctta aggaaactct tgacctgttc
    15421 cagtttcctg aaattggcta tggtaagatg ttccttaaga tatttcagct ttttaggaac
    15481 aatcacgaca atgaccttgc ttgagataaa atcttccatt tcattgacat aactcacctc
    15541 aaatgaccct ccatcatcat gcaaatccag gtattcataa cgttgtgcat tggattctgt
    15601 tacaccggat atacatttta cctgttcaat gtcatcaaac tggttcaaaa ccagtctttc
    15661 aatatcccag gctgtccagc ctctgttttt atgccttaat ctttcactta ttctggtata
    15721 gaattcgata ttgctctcct ccgcttttcc gccgaagctt tcatacggtt gaatcatact
    15781 ggttacaccg tgaattttat tgtccagagc gatgatggat tccggaggga agggagtgtt
    15841 tagccctgaa ggatcagact ggtccaccca cctggtaaga gttgcgtttg gataaatggc
    15901 cagagtatgg ttaagtaatt ggatgtcgcc cgtagcactg actcttagcc agaatttgtt
    15961 tggctccatt atctgagaca tagtagagat gtctgacgga atcttgattt gaattactcc
    16021 tgatttggta aatgagtccg tttcatccct gaggatattc tccccctgaa gtaatttcca
    16081 gttgttattg tatgtcagat attgccagcg gattataggc aggatatact gatcccgatc
    16141 ctggtaatag acaggcgtca tctgaaacaa caaagatata atctctgcct ttttacaatt
    16201 cttcaacccc agcaacaggt atccgtcttc ctcgtattgc ggaagtagat aaggcaaccg
    16261 atccctgtat ttgatatttg gatagatttt ttcgtagccg aaaggatgga tatggttgat
    16321 ttcctggaag ttgacagcat cctgggttag ttcgattaca tcagaagcct tgtaattgat
    16381 atggatatta ctcaccatcg ggatgagagg catttcgggg aatcccaatt ccggcgaatc
    16441 ttcattttca tcctctacaa atgccttgtt gataagtctg gcatttttaa gcatctgttt
    16501 tgcaagtatc tcctgatatt tttgctgccc gaaaccatct tcaggttcat ctaaaacaaa
    16561 tttgaggaaa cctacattgg cattggtaga gaattgatct gcgatttcca tggattcaga
    16621 cgaattaaag cccaacgatt tcagatctcc gctgtctaat gtatatttgg tgaataaacc
    16681 ttcatcgcta agtttgtcac ttccgatgat ttcttttgtc ataaacaatg gtctggaatt
    16741 atcttccatc acgaattggc catcattcag attgcctatc tggactttaa attcatcata
    16801 ccgcctttcg tatacttcat atttttgatt aaactgtccg gcagtttcag gcaagtcaaa
    16861 ccaatcaata tttaattcaa attcgtccag ttttttactg aaaatttccc tgttgccaag
    16921 aaggaaatag gagtttttga ctggtgtagg tccgaacgga tagaatggct gagaattgtc
    16981 gataaggcca tattcgttat ataccgataa gttgcgtaca ttttccaccc gagcatcaat
    17041 ctctatggtc tcgataacaa gtttttggag gaaggaatat agatagggtt ctctgtcatt
    17101 gttgagcaat agcatcaaaa tcggttgcga gcctgatatg tattttccgc tgtgcaaatg
    17161 ttcattgaat ggaatgatgg gaggggccat gggagacaag ttgaatgaga tacaaatatg
    17221 cctcgggtcc cattcttctt caccgtcctg aaatatgaga attgtctgtg gatccacctt
    17281 atgccatccc tttgcgctcg taagaagaat agtaatattt cttttcttac tctttgtgct
    17341 gaatatttta taaaatactt caagcatcgt acttttctta tgctctttgg aagcaatatc
    17401 tttgaaaagt ctgacaattt cggttactga ttcttccgta aaattgagtc ttattttgat
    17461 gctgcgtttg ccttctccaa gcatcaaaac ggatgatgca acggaaaagc caagctcaga
    17521 ttcaatcata tttctgagac tcggttcctt ttctatttgc ccttcaccaa atgttggcca
    17581 tgccggtagt ttatccgaat tgaacggaga tccctttcca tcctgagaat tggcgtaagg
    17641 tgccataaaa gactctgtaa ttatggaata cttatttgtc agggtttctc ttcttccccg
    17701 atcaaaatat agggtcttta tatcagaaag tgtataccgc ccgacattta caggatattt
    17761 ggtgctaaat ctatgcggga ggccatcctt atccagggat gccagcaaga tggtgttttc
    17821 cgggatggtt ttgttttcaa catagggtga aattgcaaaa cttacatagg cgtaaggggc
    17881 gatatgtttt ctctttttct gcttcagaaa atcgatatag taaaagtgaa ggtaattctc
    17941 aggaacttta ttgattgaag tttgaagctt tttgtattgt tcaaggaagc ccagcagcag
    18001 acctatatgt ggttgatgat catctttttg tgacaatgat ctcacaaaat aaggaatgag
    18061 caaatccctt attgccaagg atgtttcgag gagattattg aagaggatcc tcagtctgat
    18121 aaaaacggat tctagtttgt tcggaatatc ttcctgcgaa acatcttccg ggctactttc
    18181 aattatttgg tagcctagat tgaatgctga tttttgtttt tcagcatggg ggattgcacc
    18241 aagaatgata gcgtgcaatt catagatata atcaagtacg gattgttcgt aatacgcttc
    18301 tatgtcgaaa taaacgaatt tctcaatttc gccatcagga tattgaattt tcaggagtct
    18361 atcccttagg ttgttaaatt cagagagaag actattgacg accgaaatca tagcctctaa
    18421 agcttcgatt tggatgttgc catctgttgc ccgtgaaaat tcctttacct tcaggttgaa
    18481 gtcaaactgt gcctgagcga gatcttttgt tatgctaaga gctaaaatgg atgttagatc
    18541 gtaattaaaa aaagcttgcc aatccttact atcatcgctg tttgattctt tgaaatgcaa
    18601 cagtgatgcg tattcgtaca ggatactgag cagattttca atcggtcggt catcaatagc
    18661 gatttccagc ggattggtcc ggttttcaag cctggtagat tgtgtcgtac ctatatattt
    18721 cggatctcgg atcatgtgga ttgatacatt gactaactga tttcttcggg cagactagta
    18781 ccttcatttt tataaaatgg gaacacaata ttatgtcggg tattggttgc tcttactctg
    18841 taaataattt caatcttaac caagccatca gcgtaatcct tgatgtcggt cagaatgtcc
    18901 aatacttcga cacgtggctc gtagtcgttg atagcagtgg ttatcatttc tttgactgta
    18961 aattcgaatg ttgagtctat gggttcaaaa atcttagcat acaggtcaca tccatacttc
    19021 agatgggtga ctctttcacc gggtgtaacg gaaaacaaaa tgcgcaggct ttcggatata
    19081 tcctggaagt cgcttaccac caatgtttca cctgtattaa tattgaaatc cggcgggaat
    19141 gcccatccgg tccccagaaa cgaccttact ccgtcaattt tttttatatt tatttgatca
    19201 atatccattt aatactattt caaccgatta tcactattgc ccccgttacg gtagctatca
    19261 tttgtccgga tagtgttgca gttggtgctg aaaccatagc tgatacatta ccattaatat
    19321 taactaatgt tccggagacg gagattggtc ctgaggagtc catttttacc atggcactac
    19381 ccgtcatctg tacacccatt gttgcatcga tctccactct tcctgtagtt ttgatggtaa
    19441 ttccggaagc gtccattgtg atggaattgt tgaatccatc ttccatcttg atgtgttgtg
    19501 ctgtgtcatc cattgtaaat ttcctgccac ccggagtttc caaagttatt gatttgtcct
    19561 cttcgtggta ggtaagtttc agctctgatc ttgacacgaa accttttatt ttattgatat
    19621 cttcatgttc atagggcgat ttaagtttgg agctgtacaa ggaaccaaga atcaccggat
    19681 atctggagtc tgcattgagg aagccgacta cgacctcatc atcaatctcc ggtacataat
    19741 aggctccatg gctgtcagaa gcgtgcagtt gtgccaatct ggcccatacg ccgtcagagg
    19801 agcctgtaat cacgggtata tcgagcttaa ttctgtatcc tccttcagga tccccttcca
    19861 attgttttac cttgccaatg aatattcctt ccatagccgg tagttggccg gaggtggcgg
    19921 gattgatgat atcggttctg gactctaggt accaatcttt ttgccaacca ataatcaatt
    19981 ctgtgatcca gctcccgctt tgaagaacat gattcacttc ccggatatat gcctttccat
    20041 caaaccattt gcctaggccc ttcaattcca caattttatc aggcaggaat ttttcagtcc
    20101 cattgcaggt gatggaacct aatgttctgg acatcttcgc cctctgaaac tgggcggttg
    20161 cccattcctc caaatctttt tcaggaagat gacctgtact ttggagtaag aaatcctttt
    20221 tattgaactt gtctgccagg accgaaaatt caatttgccc ggaattgccc tggaggttga
    20281 tactaacatc ccgattaacg gattcattca gctcctgatt atcggggttc caggaaattg
    20341 tttttacttt tttaagctga aatgagccat caatttccaa ttcagattta ataatttcta
    20401 ctccatgagt caataaatgt acgggtgcag agttaaaatc aggttttttt accttaactt
    20461 tgtcgtcatc agtaagaagt atcaatccgt tttgctcaag ccttgataag ataaaatccc
    20521 aatcgcaaca tccatattgt atgatctgac ta
//
LOCUS       DAOQMC010000008        20198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_929_length_20198_cov_5.548677, whole genome shotgun sequence.
ACCESSION   DAOQMC010000008 DAOQMC010000000
VERSION     DAOQMC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20198
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_929_length_20198_cov_5.548677"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="PKV31_00730"
     CDS             complement(<1..1485)
                     /locus_tag="PKV31_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNB91400.1"
                     /translation="MFDLLHYASGIIEEADNPFIQIDLKDLDKKIDYPELLPVLPIKN
                     TVLFPGVVIPITVNRFKSIRAVEDAYTSDKMIMVLTQIKQDVENPGPDDMYTVGVIAE
                     IHKLIRMPDGSITIVIQGRSRFAVDSFEQSEPYLKGRFTILEYIQPSDTREFEAQISS
                     IKDMARNIIEISPNIPNEANNMLRNIESNTFLLNFIASNLSVDTSGKQKLLETAYLPD
                     FANMLLSNMHDEFNLLQLKGQIESKVRTDLEKQQRDYFLSQQLKTIQEELGQNTFEEE
                     HRNIEEKAAKKKWSAEVKAIFDKEFNKLKRMNPQMAEYGVQINYIETLLDLPWGEYSK
                     DNFDLKKVKKVLDDDHYGLEKVKERILEHLSVLKLKGDMKAPILCLVGPPGVGKTSLG
                     KSIAHALKRKFVRMSLGGLHDESEIRGHRKTYIGAMPGRIIQGIRKAGSSNPVFILDE
                     IDKVGKDFRGDPSSALLEVLDPEQNNSFADNFLEVEYDLSKVLFI"
     gene            complement(2171..2890)
                     /locus_tag="PKV31_00735"
     CDS             complement(2171..2890)
                     /locus_tag="PKV31_00735"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB91401.1"
                     /translation="MMKFSGAVIWGLIFAVLVVVGCKKTDNQEVATGLKDTIIVEPTI
                     PPIDSNTDREVDMATVSGNFIIEAPSTINWTASDPVSTHVGTVDVTSGNLEIKDGKIQ
                     SGSFEIDLHTINSTDLKPEDGKEKLETQLKSADFFNVEKFPSAKFVIKKSVVIKGNDK
                     TRHHITGDLTIRDVTKSIGFDAYIIVSNQGGLLTATTPSFEINRVDYGMKYKSGVINT
                     AKDKIISDKIGMLINLRAKKQ"
     gene            complement(2994..3881)
                     /locus_tag="PKV31_00740"
     CDS             complement(2994..3881)
                     /locus_tag="PKV31_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB91402.1"
                     /translation="MGLYTIKDIAQLTSIKPHTIRMWEKRYGILQPKRSENNNRYYCQ
                     SEVSLLSKIAYLTQNGHKIGKLAKLSREELARMAEDTIYNKPDEEKEDLLIKAILRFD
                     SDTIGDTIYRSMEAAGAYETFINMLLPIAGRLNLLMMAGRIRQSHIGHFFNVVKKIFI
                     SEAAKYPPNKSCNTIHLVLYPFRSSTQYISMAAIECGLRQHGIRCTELGQNCPVEDLS
                     EVFISLKPDAFVTVLYEDVATNEFYNIINRMNGFIGNTPLYIVSDEDMKEFPRLPLNV
                     RVFPDVSTLVEYARSLKRD"
     gene            4000..5145
                     /gene="bshA"
                     /locus_tag="PKV31_00745"
     CDS             4000..5145
                     /gene="bshA"
                     /locus_tag="PKV31_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNB91403.1"
                     /translation="MKIGIVCYPTYGGSGVLATELGIGLADHGYEVHFITYQKPVRLS
                     SFHQNVYFHEVEQVDYPLFEYAPYESALASKMVDVVRYEKLDILHVHYAIPHATVAYL
                     SKQILARHGIRIPIITTLHGTDITLVGINKTFTPIIEFSIDASDGVTAVSNDLKQQTI
                     ENFDIKHEIRVIHNFINFNRFRKSNKDHFRKAIAPNGEKIITHTSNFRPVKRVGDVVD
                     IFARITKVLPAKLLLIGDGPMRSELEERTRNCPQYENIRFLGKQDAVEEILAISDLFL
                     MPSEKESFGLAALEAMACEVPVISSNAGGIPEVNIDGVTGFLSDIGDVNAMAHNALKL
                     LQNDELLHQFRQNALFQARQFDIDRILPQYIRYYEEIIEKVNAEQVV"
     gene            5538..6518
                     /locus_tag="PKV31_00750"
     CDS             5538..6518
                     /locus_tag="PKV31_00750"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNB91404.1"
                     /translation="MLCIYHESTDPYFNIATDEYILKYMEEDCFMLWRNDNAIIVGKY
                     QNTLSEINYDYVRENNISVVRRMSGGGAVYHDLGNLNFSFTKSGADSNLTDFEKFTKP
                     IVDVIRSLGVDARFEGKNDLMIDGRKFSGNAAHIYKNKILHHGTILFASEMRNVAEAL
                     RINPVKYQDKAQKSVPKRVTNISEHLREPMTVKEFTEKVMDYVLSIYPDSRLYQFTEE
                     DLAIIRKLRDEKYATHEWNFGKSPDYSFKKAIRTLGGLLEMNIEAKKGVIEDVKIFGD
                     FFSERGIDEIEQALVGVHHEEKAIRGVLSRFDIGLYFSNITVDNLIEAMF"
     gene            complement(6520..6996)
                     /locus_tag="PKV31_00755"
     CDS             complement(6520..6996)
                     /locus_tag="PKV31_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91405.1"
                     /translation="MVFTEKMIERVFDREVDSELEPMVVLNAIAGDQPAIIAYLNQEN
                     LQLLTEEEFDYCTFLTTIIYKVFRENGVEVAEVTPAQIDAAEEANWDILSRATGKKFS
                     QRIDGFFENYVQEDLLAFVEDALEPDELPIVTPEGREPLFITLKTIIDVLNANIKV"
     gene            complement(7082..7891)
                     /locus_tag="PKV31_00760"
     CDS             complement(7082..7891)
                     /locus_tag="PKV31_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91406.1"
                     /translation="MRQYPSKIILFGEHTLLWGSEGLAIPGNQYFGHWAQGNPRHQGT
                     DLYSFADYLSGQKFHNRVDHAGLLEDATNGWFFDSNIPVGYGVGSSGAYSAAIYDRYA
                     CVTDKDASHIRQDLAAIESFFHGSSSGIDPLISYYDRSFHLKDGYPEEVVIADRIRDS
                     FTLTDTGIKRDGSDYIRLFAQKMEDPNFAQKIKELYIPLVTNAIDACLKDDIGRLHEH
                     FFEISYFQLVWMTEFIPFHLIKKWEQQLSDYTGYYKICGAGGGGFLLKIRK"
     gene            8509..9585
                     /locus_tag="PKV31_00765"
     CDS             8509..9585
                     /locus_tag="PKV31_00765"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91407.1"
                     /translation="MKETLERLSEITAPVCVTLILNTHRTTPENQKDIIALKNLISET
                     YERLQREHGTDIAKRYTAKLQGLAEDIDHYHNDHGLMLFVNDEIAEYLRLPIHVTDRV
                     IIDKTFATRSIVRAMKRVSDYYVLVLSKGKARLIEASGDVVVKEVRGEGFPFNDSQFM
                     KLSSAESSNAARASNLAHEFFNRVDKALNKIRNENPLPVVVCAEESNYHLYLAAADHP
                     NIILGHVLLKNIDASPNSIVKDYVAPQVEEMTLLKHRERISELSAAVNSGRALTDLNE
                     IWAAVRVGRGKTIFVEQGYIQAVRKEDGLLKPIALDEVDSKEDINDVVDEMIEHTMKM
                     GGDVVFVRKGDLEQFNKLALATRY"
     gene            complement(9672..10895)
                     /locus_tag="PKV31_00770"
     CDS             complement(9672..10895)
                     /locus_tag="PKV31_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNB91408.1"
                     /translation="MKYLIGSLFFVLSFYSCISTKDDINDNKPSVSINENTTKGLQSG
                     AVDIFRQFVRSSYEDDNVIISPLSIQYAFGMAANGASGSTLDEIMRLMGTPSSGLQEV
                     NTGLKDIMQNLKADDKDFTISIANGVFYDPNMFRFNATYEKSLLNLYNAELLQSNFDD
                     KEATVNAVNKWASDHTRERIQKVLDDVQQGEFMFLLNALYMKASWDKGFPPELTVKQN
                     FTTSKNIQNQVDMMYRHEPAILHRNNDEIAVVVPMGNGRLEALFILPNQDGITTYIDK
                     LNRDKIEYFFGKGQVTDMFLKLPKTELLLHYDLKKILLDMGIVLPFSSTADFTKMGTA
                     SGRINLSRAMHDVFMKMDEKGMEGAAVTTIGVENTSVPIAEEVSFDRPFVMVVRDKNS
                     GNYLFMGKIENPAKK"
     gene            11179..11514
                     /locus_tag="PKV31_00775"
     CDS             11179..11514
                     /locus_tag="PKV31_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91409.1"
                     /translation="MKKLLFLFIISLGMISCHKEEIKGDLFTDSCVQSFISKNNLQIV
                     SVDKADCIFYSIYQYEGRFYYEFNCCVCDLIPQIVNCDGSLYLEYDWDKIQAFKKKCT
                     VLPYILVVK"
     gene            12188..13657
                     /locus_tag="PKV31_00780"
     CDS             12188..13657
                     /locus_tag="PKV31_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNB91410.1"
                     /translation="MKLLFLFLFNVFCLMLQAKVWHVGPNRDYTRPSQVSSLVDHGDT
                     VYIDPGVYDNDACKWNKNKLKLIGIVGDNGRPVLRYEGYVPNDKGIWVFDNPGSSDDI
                     YIANLIFDGARVPDNKGKNGAGIRFQANNLTVENCLFRNCQTGILEGHGAVKTSNVII
                     RGCEFFDNGYSGYEHHVYINASTDTLVIENCYLHRPRGEGNSIKTRAQNAYILFNFID
                     EEDGNGSYEINIAQGGNNIIMGNVIVQGTSGGNHAIVGYDEALNEQEDFYFINNTVIN
                     RFPGHCRYFNIAPATGIATFRVYNNVFASVPTASHTFISGNVPAVLDSAANYFATDYT
                     QVGFVDPDQGDYSIHGAASGLINQGVAAGTDKHGYPLVPDSMYQGIGKQLLPRIIHGQ
                     AIDLGAYEYATTEKAATIHPLVSVFPNPNTGTFEIRVNPVSKSLPKIYNSAGMKVDAG
                     ISASQEGMIRVCLSGAFPGIYYVVFVSENGSTSVPVMVY"
     gene            14288..14893
                     /locus_tag="PKV31_00785"
     CDS             14288..14893
                     /locus_tag="PKV31_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNB91411.1"
                     /translation="MKKCLLTTLFIGAFLYVADAQTQRVDTVFFGGKKMLRVITEKGD
                     TDYLDQLSEVSISSKQQFSSKEEYDRYMRYRRYAAIVYPYALEAMRIYHESREATRSM
                     TKRERKKYMKTLEKELEDQFEEPLKKLTRTQGLILTKMIEKELDEPFYDVIKEIKGGW
                     SAFYYNTTGKFYGYHLRDGYERGKDYILDMVLDDYRLSVKK"
     gene            complement(15302..16033)
                     /locus_tag="PKV31_00790"
     CDS             complement(15302..16033)
                     /locus_tag="PKV31_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNB91412.1"
                     /translation="MENIIENIRAIRTRIAEACTRVGRHPDEVRLLLATKTVIPQNIK
                     LAFAAGETLIGENKVQEIKEKYDDLKSITHEMHFIGHLQTNKIKGIIKYNVDCVQSID
                     RFDLADKLNHRLLQTGRAMDVLIQVNTSSEESKFGVAPEEAVDLVYSVSQLSNLRIKG
                     LMTIGLFTNNPEQARPCFRLLSDLRSELNRLNFPGVEMNELSMGMSGDLETAIEEGAT
                     IVRVGTAIFGLRIYPDSYYWNEGKT"
     gene            16221..17270
                     /locus_tag="PKV31_00795"
     CDS             16221..17270
                     /locus_tag="PKV31_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB91413.1"
                     /translation="MSELVLTIDGVEPLELLGENNSKLQLVKARYPDLDISSRGNNIK
                     ISGDKKLTQELKSKIEILVRYLKENKGLPVTIVDTILSGSNPYNDLAIEANSNGGNVI
                     VHNKDGFPIRARTVNQRKLVDATERADIVFAVGPAGTGKTYTAVALAVKALKNRLVKK
                     IILTRPAVEAGESLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLAKFMSDRIIEVAP
                     LAYMRGRTLDNAYIILDEAQNTTSPQLKMFLTRLGPNAKIIVTGDLTQIDLPYSQQSG
                     LKRALSILSDVEGIAQIYLNEEDVVRHRLVKDIIRAYNVSDAEIRAKKEKERTEREAA
                     KEATHSAIDKINNDL"
     gene            17272..17775
                     /locus_tag="PKV31_00800"
     CDS             17272..17775
                     /locus_tag="PKV31_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB91414.1"
                     /translation="MKGIHSMSLIILMVSTAILSKAQETKLYNERADATQEIAMAVTI
                     AHKNNQHVLIQGGGNWCSWCIKFNKFTREDAQIDSMIRADYQVVHLNYSKENKNEAIF
                     AQYGFPQRFGFPVFIILDGAGNRIHTQNSEYLEDGKSGYDRKRVIEFLKAWSPAAIDP
                     KTYQSKK"
     gene            17782..18183
                     /locus_tag="PKV31_00805"
     CDS             17782..18183
                     /locus_tag="PKV31_00805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB91415.1"
                     /translation="MTSKVIYKGHLETECIHHKSSESIRTDAPTDNNGKGSAFSPTDL
                     CATSLATCMLTIMGIWARDNEVDMDGAYAEVTKHMGTDPRRIVRIDVNLHFPDMNYSS
                     RVQSVFKNIAKTCPVAYSLHPDIEQVVTFNW"
     gene            18199..18867
                     /locus_tag="PKV31_00810"
     CDS             18199..18867
                     /locus_tag="PKV31_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNB91416.1"
                     /translation="MATSIQPSTLHFLIDLSINNYRVWFEDNREAYKLARANFLDFIQ
                     EIIDDLAQDDDSVHGLQAKDCAFRIYRDVRFNKDKSPYKVHFGASIKPGGRKSGKAGY
                     YFHLEPGKSFIGGGIYNPESDQLSHIRKKISGDYSGFTKILAEEKFRSLYTDIDRTDK
                     YLLKRVPRGFKATDPAAGYLLLTSYLSTINLDDDLLVSPTLHKYTVGAFQALHPVIRF
                     LNEV"
     gene            complement(18987..19883)
                     /locus_tag="PKV31_00815"
     CDS             complement(18987..19883)
                     /locus_tag="PKV31_00815"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB91417.1"
                     /translation="MNDFHLNQLYKVLTHIDNNLNSDLSLSTIATVGNYSVFHFHRLF
                     KAYTGETLNEYITRKRLENIAAILIRDKTISITQLAYLNGFSSNASLTKTFKKMYNIN
                     PSQFKKKSSSQYDKIIKSKNGQTFEKFDQYLWRINNLINWLHMNATVNVMDLTPIKLV
                     FVSHIGIDGLDDAFSRIFEWASLKELTNGDNVEFVRIYNDSFKITSPNKVRMEIGIPV
                     HQKVTPDSGIQFKEFEPRRCIVGNFEINLPDFEKAWSSLFIWMNENGYKPGKQRPFEI
                     IKNNFNKHPQKKCIVDLYIPVE"
BASE COUNT         5842 a         4158 c         4488 g         5710 t
ORIGIN      
        1 gatgaataat actttggaca aatcgtattc tacttcgagg aagttatcgg caaaggaatt
       61 gttttgttcc ggatcgagca cttcaagcaa ggcagaagag gggtctcccc tgaaatcttt
      121 tcccaccttg tctatttcgt ccaggataaa taccggattg gacgagcccg ccttgcgtat
      181 tccctgaatg atccttcccg gcatagctcc gatgtaggtc tttctgtgac ctctgatttc
      241 ggattcgtca tgaagaccac caagagacat cctgacaaat ttgcgcttca atgcatgggc
      301 gatggatttt ccgagtgatg tctttccgac tcccggaggg cctaccagac aaagaatggg
      361 tgctttcatg tcgcccttta acttcaggac agaaagatgt tcgaggatac gctccttgac
      421 tttttccaaa ccgtaatgat cgtcatccaa caccttctta acctttttta gatcgaagtt
      481 gtccttagaa tattcacccc aaggcaaatc cagcagggtt tctatatagt tgatctggac
      541 gccgtattcc gccatttgtg gattcatccg cttgagctta ttgaattcct tatcaaagat
      601 ggctttgact tctgcagacc atttcttttt tgccgctttt tcttcgatgt ttctgtgctc
      661 ttcctcaaaa gtattctgcc ccagttcttc ctgaatggtc ttcaactgct gagacaggaa
      721 atagtcacgt tgttgctttt caaggtcggt acgcactttt gattcaatct ggcctttgag
      781 ttgaagtaga ttgaactcgt catgcatgtt agacaatagc atgttggcaa agtccggcaa
      841 ataggcagtt tcaagcaact tttgttttcc ggaagtgtcg actgacagat tagaggcaat
      901 gaaattgagc aggaaggtat tgctctcgat gttgcgaagc atgttattgg cttcattcgg
      961 gatattgggt gagatttcga tgatgttcct cgccatatcc ttgatcgatg atatctgcgc
     1021 ttcaaactca cgcgtatctg acggctggat gtattccaat atcgtaaacc gacctttcag
     1081 gtagggctca gactgctcga aagagtccac cgcaaatcgg gaccttccct gaatgacgat
     1141 tgtgattgaa ccgtcaggca tacggatcaa tttgtgtatt tctgctatca cacccaccgt
     1201 atacatatca tccgggccgg gattttcgac atcctgcttg atctgcgtca ataccataat
     1261 catcttatca gaggtgtaag catcctctac agcgcggata gatttaaacc tgttgaccgt
     1321 gatgggaatt accactcccg gaaacagaac cgtattttta attggcagta caggcagcag
     1381 ttccggatag tcgatctttt tgtccagatc cttcaaatct atttgtatga aaggattgtc
     1441 cgcttcttca ataattccgg aggcataatg caataaatcg aacatgtaat tatttttaag
     1501 gctgaatcag ccgattattc aaaactattt gggcttttta agtattgcaa aagccattcc
     1561 attaaggatt gtgcgacaaa aaggcagccc gccccctcaa ggtattgcct ggtgcctgat
     1621 ttcaaggagc cggatctttt gtaagggccg gatctttcag gaatattgtc ggtcgagcgc
     1681 cgagataaat gtgtgttgac cgaagaaacc agcgaatacg acgcgatttt cttctaaatg
     1741 attgtgctta tcaaccggga gcgtcccaaa aggacaaaat ggcagttgta ttaattgtct
     1801 aagcacaacg agggtcagga gttatgaaga tgtctcatgg acaacttcac atgctttcgc
     1861 cgggtggata acagccgggt gtgtatccat cagataagtc attgtgcaac aaggggatga
     1921 tcgcccgaca gtccaattat tctcgtgttt gccttcttcg cgaatagaaa aaaaataaat
     1981 attgtggaag ggtatttgcc cgttatgtcg agccagattg tagaggaggc atcaatattg
     2041 caaaacagct ttgctcaaaa aatagaatta tccctaatta tcgctgcttt agtcaagttg
     2101 aagttgattc aacaatctca tatatcaaga atttacttcg cctataaggg aaaccagagc
     2161 cggttgctat ttactgtttc tttgcccgga ggttgatcag catgcctatc ttgtctgaaa
     2221 taatcttgtc ttttgcagta ttgatgacac cgcttttgta tttcatgccg taatccaccc
     2281 ggttaatttc gaatgaaggg gtagtggcgg tcaacaaacc accttggttt gacacgatga
     2341 tataggcatc aaaaccaatc gacttggtaa catcacgtat tgtaaggtca ccggtgatat
     2401 gatgtcttgt cttatcatta cccttgatga cgactgattt ttttatcacg aacttagctg
     2461 aagggaattt ttccacattg aaaaaatcgg cggacttcaa ctgtgtttca agtttttcct
     2521 tgccgtcttc cggtttcaaa tcagtagaat taatagtatg aagatctatt tcaaaggagc
     2581 cggattgaat cttgccatct tttatttcca ggtttccgct tgtcacatcg acggtaccta
     2641 catgtgtaga gaccgggtcg ctggcagtcc agttgatggt gctgggagct tcaatgataa
     2701 aatttccgga tacggtcgcc atatccactt ctcggtcggt gttgctatcg atggggggaa
     2761 tggttggctc gactataatt gtatctttta gtccggttgc aacctcctga ttgtctgttt
     2821 ttttgcatcc tactactaca agtacagcga aaatcaatcc ccaaattaca gcgccactaa
     2881 atttcatcat tcattctatt tagaaaattc aataaaaggt cgatttgaca aagtaaagtt
     2941 atattaattt gccaatttgg gagaaaacta aatgtcaaaa tattttaccc cggtcaatcc
     3001 ctctttagtg atctggcgta ttccaccagc gtgcttacat cggggaagac tctcacattt
     3061 agcgggagtc gcgggaattc cttcatatcc tcatcggaaa ctatgtacag gggtgtattg
     3121 ccaatgaatc cattcatcct gttgatgatg ttgtaaaatt cattggttgc gacatcttcg
     3181 tacaacactg taacaaaggc atccggcttg agggaaatga aaacttccga cagatcctct
     3241 accggacaat tctgacccag ctccgtgcac ctgattccat gctggcgcag accacactcg
     3301 atggctgcca tactgatgta ttgtgtactg ctcctgaacg ggtaaagaac caggtgaatt
     3361 gtattacaac tcttgttggg cggatatttt gcggcttcgc tgatgaaaat cttttttacc
     3421 acattgaaga aatggccgat atgtgactgc cttatcctgc ctgccatcat cagaaggttg
     3481 agccttccgg caattggcaa cagcatgttg atgaaggttt cgtaagctcc tgctgcttcc
     3541 atcgaacgat atatcgtgtc gccaatggta tctgaatcaa accggagtat tgccttgatg
     3601 agcaggtctt ccttttcctc atcaggcttg ttgtagattg tgtcctcagc catcctagcc
     3661 aattcttccc gggacagttt tgccaactta cctatcttat gtccgttttg agtcaggtaa
     3721 gcaattttag aaagcaatga tacttcggat tggcaataat atctgttgtt attttccgac
     3781 cttttgggct gcaggatacc atacctcttc tcccacatcc tgatagtgtg tggcttgatg
     3841 gatgtgagtt gtgcaatatc cttgattgta tatagtccca aaatttatct cctctttttg
     3901 cattaaaaca atccattaaa ttaaattgtt tatagccgta aagtaggact catctgtaaa
     3961 aagcggtgtg cttccacttc aacttttcaa atacagaata tgaagatagg tatcgtatgt
     4021 tatcccactt atggcgggag tggcgttttg gcgactgaat tgggcattgg actggccgat
     4081 catggctatg aggttcattt cattacctat caaaaacctg tcaggctcag ttcttttcat
     4141 cagaatgtct attttcatga ggtagaacag gtggattatc cgttgtttga gtatgcaccc
     4201 tatgagtctg cccttgcttc caagatggtc gatgtggtgc gttatgaaaa gctggatatt
     4261 cttcatgttc attatgctat accccatgct acagttgcat atctttcgaa gcagatactg
     4321 gcacgccatg gtattcgcat tcccatcatc accacgctgc atggtactga tatcaccctg
     4381 gtcggtatca ataagacttt tacccctatc atagaattca gcattgatgc aagtgacggc
     4441 gtcaccgcgg tatccaacga ccttaagcag caaaccattg agaacttcga cattaagcat
     4501 gagattcgcg ttatccataa ttttatcaac ttcaatcgct ttcgcaaaag caacaaggat
     4561 catttcagaa aagcaattgc accaaacgga gagaagatta tcacccatac ttccaatttt
     4621 agaccggtaa aacgcgttgg cgatgtagtg gatatttttg cccgcattac gaaggtattg
     4681 cccgctaagc tgttgcttat cggggatggc ccaatgcgta gtgaactgga agaacgtaca
     4741 cgaaattgcc cgcaatatga aaatatccgc tttctgggta agcaggatgc cgtggaggaa
     4801 attttagcaa tcagtgatct tttcctgatg ccatccgaaa aggaaagttt cggcctcgct
     4861 gccctggaag ctatggcctg cgaagtaccg gtcatcagtt caaatgccgg gggtatcccc
     4921 gaagtgaata ttgatggagt gaccggcttt ctcagcgata ttggagatgt gaatgccatg
     4981 gcccataatg cgttgaagtt gcttcaaaat gatgaactgc tccaccaatt caggcagaac
     5041 gccctttttc aagcccgaca atttgatatc gaccgcatat tacctcaata tatccggtat
     5101 tatgaagaaa tcatagaaaa agtcaacgcc gagcaggttg tctgatcaaa tattttgtga
     5161 ggaagctgcc cgttgttcaa atttttttct ttaatgatgt aaagtatagt ccattccagg
     5221 tatcccgctt tctataatat acagttgccg tagtagaacc gatgacatgc agcttttctc
     5281 atcacagaca ctaccctaag gtgttttggc aattcatccg tagtcggtat gctcgttaat
     5341 gccgaatatg ctgtgcctgt cttcaaaagt attgatattg tccccgataa tacgcacagg
     5401 gtacagatca gaagatgtgg tgcctggcct ccggcaggga cacaccgaat caaggcaaat
     5461 atgtgtttca cggaatcaat tagcatattt atcgctcttt ttttacttaa ttttgcgttg
     5521 caaaaaatta attcgacatg ctgtgtatat atcatgaatc aaccgacccg tacttcaaca
     5581 tcgccaccga tgaatacatc ctaaaatata tggaggagga ttgcttcatg ctgtggcgta
     5641 atgacaatgc aatcatagtg ggaaaatacc aaaacacgct gtcggagatc aattatgact
     5701 atgtcaggga aaacaatatc tctgtggtaa gaaggatgtc tggtggcgga gcagtgtatc
     5761 acgacctggg caacctgaat ttttctttta ccaaaagcgg agcggattct aatcttaccg
     5821 attttgagaa attcaccaaa cctatcgtgg atgtcatccg ttcattgggc gtggatgctc
     5881 gttttgaggg aaagaatgac ctgatgatcg atggcaggaa gttttccggt aatgctgcac
     5941 atatatacaa gaacaaaatc cttcaccatg gcaccatcct gttcgcttca gaaatgcgta
     6001 acgttgcaga agccctgaga atcaacccgg tgaaatatca ggacaaggcc cagaaatcag
     6061 tgcccaaacg ggtgaccaat atctctgagc acctcaggga gccgatgacg gtcaaggaat
     6121 ttaccgaaaa agtgatggac tatgtacttt ctatatatcc ggatagccga ttgtatcagt
     6181 ttaccgagga ggacctggcg atcatccgca agctcagaga cgaaaaatat gcgacacatg
     6241 agtggaattt tggcaagtct ccggattaca gttttaaaaa agcgatcaga acattaggtg
     6301 gcttgctgga gatgaatatc gaagcaaaaa aaggtgtcat agaagatgta aagatctttg
     6361 gggacttttt ctctgagcgc ggtatcgatg agatcgagca ggcccttgtc ggagtccatc
     6421 atgaggaaaa ggccattcgc ggggtgctca gccgctttga catcggccta tatttttcca
     6481 acatcacggt tgacaatctg atagaggcca tgttttgacc tatactttta tattggcatt
     6541 gaggacgtcg atgatggttt tgagcgtaat aaataatggt tcacgacctt cgggggtcac
     6601 gatcggcagc tcgtccggct ctaaagcatc ttcgacaaag gcgagcaggt cttcctgcac
     6661 ataattttca aaaaatccgt cgatacgctg gctgaatttc ttgccggtgg cacgggataa
     6721 gatatcccag ttggcctctt cggcggcatc aatttgtgca ggagttactt cggccacctc
     6781 cactccgttc tccctgaaca ccttgtaaat gatggtggtg aggaaggtac agtaatcaaa
     6841 ttcttcttcg gtcagtagct gtagattttc ctgattgaga taggcgatga tggccggctg
     6901 atccccggca atggcgttga ggaccaccat cggttccaat tcagaatcca cttcccggtc
     6961 aaaaaccctt tcaatcattt tttcagtaaa gaccatatcg ttaagtttaa tttattcttg
     7021 ctttgaatga aattgatacc catcttgcaa gcgtccggca ttcacagaat gagttcttgt
     7081 actattttct tattttaagc aggaatccac cacctccggc accgcatatt ttgtaatatc
     7141 cggtatagtc ggacaattgc tgttcccatt ttttaatcaa atggaaggga atgaattctg
     7201 tcatccacac caactgaaaa taacttatct caaagaaatg ttcatgaagt ctccctatat
     7261 cgtccttcag gcaggcatcg atggcattgg tcaccaaggg aatgtacaat tctttgatct
     7321 tttgggcaaa attaggatct tccatttttt gggcaaacaa tctaatatag tcggagccat
     7381 cccttttgat gcctgtatcc gtcagggtga aggagtccct aatccgatcc gcaataacaa
     7441 cctcttccgg gtagccgtct ttcagatgga aagatcggtc ataatatgaa atcagggggt
     7501 caatacccga actggaacca tgaaaaaagg attcgatggc agcaaggtct tgccgaatgt
     7561 gtgaagcgtc cttgtcggtc acacaggcat acctgtcgta aatggcggca ctatatgccc
     7621 cggaagagcc gacaccatag cccaccggaa tattggagtc aaaaaaccac ccattagtgg
     7681 catcttcgag caatccggca tgatcaaccc ggttatgaaa cttttgtccg gataaataat
     7741 ctgcaaagct gtacaagtcg gtaccctgat gtcttggatt gccttgtgcc cagtgcccga
     7801 aatactgatt gcccggaatg gccaagccct cactgcccca taacagggta tgttcaccga
     7861 aaagtatgat cttggaggga tattgccgca tcacgtcctc tgtttaccgg aagctaaggt
     7921 aaatgtagcg caggaaataa tgaaataaaa ttacgtttgg aagaaacacc cttgaaatca
     7981 gggcggaatc catcagacag gatcgtatta atcaagggtc gtcattagct caaaaaatct
     8041 atctgtgcca tttcgggtaa ggaaaatggt aaaaaccaaa taagattaaa gtctgacaaa
     8101 cacgcgtatt tccgaattga aaattgctaa aatttaattg tcataattaa gttttaaaac
     8161 ggtctgtttt tttgcaataa aatatttttc gaaattgcat aaacaactga tatataggat
     8221 gttttgtatt aaatacggaa cttttgacct gttaatttcg ttgaaattat agcgtatatt
     8281 tgatatgaaa ccgaaagcaa atatttcaat catcaggtct gtgaaggaat tgtcctgatg
     8341 agcaaataag ggtagtaata aattccagca aagcataatt acatagggca ataaccagat
     8401 tgggtgagtc cggaaagaga ggtaggtccc ttctacctaa tgatattggt atgtgtaatt
     8461 ggcaaaagaa ggaaaaaatg aattgtaaat agtcaaaaat tgaaaagcat gaaagaaact
     8521 ttggaaagat tgagcgaaat taccgctccg gtatgtgtga ccttgatttt gaatactcat
     8581 cgaaccactc ctgaaaatca aaaagacatc atcgccctca aaaatctcat ctccgagacc
     8641 tatgaacgat tgcagcgtga gcatggaaca gatattgcca agcggtacac ggccaaactg
     8701 cagggactgg ctgaagatat tgaccactat cacaatgatc acgggttgat gctgtttgtc
     8761 aatgatgaga ttgctgaata tctgagattg ccgattcatg tgacagacag ggtgattatc
     8821 gacaaaacat ttgctacaag gtcgattgtg cgggcaatga agcgagtatc cgactactat
     8881 gtactggtac ttagcaaggg taaagccagg ttgattgaag cgagtggcga cgtagtagta
     8941 aaagaggtca gaggagaagg attcccgttc aatgacagcc agttcatgaa attaagcagt
     9001 gcagaaagct ccaatgctgc cagggcttct aacctggctc acgaattctt caatcgtgtg
     9061 gacaaggccc tcaacaagat aagaaacgaa aatccactgc cggtggttgt atgtgctgag
     9121 gaatccaatt atcaccttta tttggcggcg gcggatcatc caaacatcat actgggacat
     9181 gtattgctca agaatattga tgcaagtccg aacagtattg taaaggacta tgtggctccg
     9241 caggtggaag aaatgacact cttaaagcat agagagcgca tcagtgaatt gtcggcagcc
     9301 gtcaacagcg gtcgggcatt gactgatctc aacgaaatct gggcagcggt aagggtcgga
     9361 cgcggaaaaa ccatttttgt ggaacaggga tacattcagg cagtgcgtaa ggaggatggt
     9421 ctgttgaagc caatcgcatt ggatgaggtg gacagtaagg aagacattaa tgatgtcgtg
     9481 gatgaaatga tagaacatac gatgaagatg ggaggcgatg tggttttcgt aagaaagggt
     9541 gacctcgaac aattcaataa actcgctttg gcaaccagat actgatctgc aatttcagag
     9601 gatagtttgg ataaaaaaag agaggcagaa gcctctcttt ttgtttttgt attataaaca
     9661 gattcaacaa atcatttctt ggccggattt tcaatcttgc ccataaacaa ataatttccg
     9721 gaattcttat cccggacaac catcacaaac ggcctgtcaa aactcacctc ctctgcaatg
     9781 ggcacggagg tgttctctac accaatggtg gtgaccgctg ctccttccat tcctttttca
     9841 tccattttca tgaaaacatc gtgcatcgcc ctggaaagat tgatcctgcc ggaggctgtt
     9901 cccatctttg taaaatcggc tgtggaagag aatggaagca cgatgcccat gtctaacaaa
     9961 atcttcttca gatcataatg taacagtaat tctgtcttag ggagcttgag aaacatatcc
    10021 gtcacttggc ctttcccaaa aaagtactca attttatccc ggttcaactt atcgatatag
    10081 gttgttatcc cgtcctgatt ggggaggata aacaaagcct caagccgacc attgcccatg
    10141 ggaaccacca ctgctatctc atcgttgttt cgatgcagga tggcaggctc atggcgatac
    10201 atcatatcta cctggttttg aatatttttt gatgtcgtga aattttgttt taccgttaat
    10261 tcaggcggga atcccttgtc ccagcttgcc ttcatataca gtgcattgag gagaaacatg
    10321 aattcaccct gttggacatc atcaagcact ttctgaatcc tttcccgggt gtggtcggaa
    10381 gcccatttgt tgacagcgtt gaccgtggcc tctttatcat cgaagttgga ctgaagcaat
    10441 tcggcattat acagattgag caaggatttt tcatatgtag cgttaaaccg gaacatattc
    10501 ggatcataaa acacgccatt agcaatcgat atggtaaaat ccttgtcatc agctttcagg
    10561 ttttgcatga tgtctttcaa gccagtattc acttcctgta ggccggagga aggagtgccc
    10621 atcagtctca ttatttcgtc tagcgttgag ccggatgcgc catttgcagc cataccgaaa
    10681 gcatactgga tacttagtgg agaaatgatg acattatcat cttcataaga cgagcggaca
    10741 aattgtctga aaatgtcaac agcgccggat tgaaggccct ttgtcgtatt ttcgttgatg
    10801 gagacagaag gcttgttgtc gttgatatca tccttggtgg atatgcagga atagaaggac
    10861 aacacaaaaa aaagtgaacc gattagatat ttcataacaa attgattttc tattattaac
    10921 gcaaagaaaa gggaagacgt ttgccctagg acgattttca tgaaataaat atgatcattc
    10981 gggaatttta ccgattgatt ccattgaatg aagtttctgt tagaaacatt ttagttaatt
    11041 caagctgatg actcattaca taatacaata agggatgtcg ggcaaacagg cctaaagaaa
    11101 attaaataga aacaaatata ttatcaacca gctaacctaa gagcttttcc catcgttata
    11161 tatccaaatg gacttcatat gaaaaagctg cttttccttt tcattatttc cctggggatg
    11221 atatcctgtc acaaggaaga aattaaaggc gatttattta cggattcctg tgtacagagt
    11281 tttatttcga aaaataatct tcagatagtt tcagtagata aagcagactg tatattttat
    11341 tccatttatc aatatgaggg acgattctat tatgaattca attgttgtgt ttgcgacctg
    11401 ataccacaga ttgtcaattg tgatggaagc ctgtatttgg agtatgattg ggacaagatt
    11461 caagccttca agaaaaaatg tacggtactt ccctatatcc ttgtagtgaa ataaggattt
    11521 gacaaattca cctgcaacag gatgcttttt ttaaatattt aaaaagtcgt ttctcaaagc
    11581 ataaataccc gactcataca agggtcattg gataatccct tattgaggag aattctattc
    11641 gcactgtttt cagccatgta atcaatcaga agcatgggag agaaaaaata ttttcttagg
    11701 aatcacggag atgtgaaaga cgtcctcaga tcggcttatt gttccgatta cacaggattc
    11761 tctaatattt tgtgacacag cattttatca tgcaatttca aaggaagcat atttttgaat
    11821 ctacttatct aagtacgctt ttaaaggaat atttcaatat ggaatgaagt ttcaatagtg
    11881 ataaattccg aaggataata ataaaacagg cttacgatca cgataattct cccggttaat
    11941 agaaatcaag cccctaaata aaaattgttg attgctgcag attggtaaga tagcaggggt
    12001 tttctttatt aaaataccaa aaaggaaaaa gaaagattga gtaaaaagtt gtttcgtaac
    12061 gcagcgattc aacagacaat agtatattta cattttgagt tgacagtttg tcataaattg
    12121 tgtataaata aattttaagt ccaccctaag caattccctg ctttttaata cttaaacctc
    12181 tatcaaaatg aaattattat tcttgttttt gttcaatgtg ttctgtttaa tgcttcaggc
    12241 caaggtgtgg catgtcggtc ctaaccggga ctacacccga ccaagccagg tgtcatcact
    12301 ggtggatcat ggcgatacgg tatatatcga tcccggagta tatgataatg acgcttgcaa
    12361 atggaacaaa aacaagctga aactaatcgg aattgtgggt gataatggca ggcctgtact
    12421 gcggtatgag ggttatgtcc ccaatgacaa gggtatttgg gtgtttgaca atcccggaag
    12481 ttctgatgat atctatatcg ccaatctgat atttgacgga gccagggtgc ctgataacaa
    12541 aggtaaaaac ggtgccggta tccgttttca agcaaataat ctgactgttg aaaattgtct
    12601 gtttcgcaat tgccagacgg gcatcctgga agggcatgga gccgtgaaaa ccagcaacgt
    12661 catcatacga ggatgcgaat tttttgacaa cggctattcc ggatatgaac accatgtata
    12721 tatcaatgca tcgacagaca ccctggtcat cgaaaactgc tatcttcacc gacctcgcgg
    12781 ggaaggtaat tctatcaaga ccagggcaca gaatgcgtat atcctgttta atttcataga
    12841 tgaggaagac ggtaatggca gctatgaaat caatatcgcc cagggcggca acaacatcat
    12901 catgggcaat gtgattgtcc agggaacttc aggtggcaac catgcgatcg ttggatatga
    12961 cgaagcactc aatgagcagg aggactttta tttcatcaac aacacagtca tcaaccgatt
    13021 ccccggtcac tgcaggtatt tcaatattgc accggcaaca ggaatagcga cattcagggt
    13081 gtacaataat gtatttgctt cagtacctac agcgtctcat acgtttatct ccggtaatgt
    13141 gcctgcagtg ctggacagtg ctgccaacta ctttgccacg gattatactc aggtcggctt
    13201 tgtggatccc gatcaggggg attattccat ccacggtgcc gcttccggtc tgatcaatca
    13261 gggtgtagca gcaggtacag ataaacacgg ctatccgctt gtgccggaca gcatgtatca
    13321 gggaatagga aagcagctgt taccgagaat catacatggt caggcgatcg atctgggtgc
    13381 gtatgaatat gcaactacgg aaaaagcagc aacgatccat ccattggttt cagtatttcc
    13441 caatccaaat acaggaacat ttgagataag ggtgaatcct gttagtaaga gcctaccaaa
    13501 gatttacaat tcggcaggga tgaaagtaga tgccggaatt tctgcctctc aggaaggaat
    13561 gatcagggtc tgcctttccg gagcctttcc gggcatttac tatgttgttt tcgtatcgga
    13621 aaatggcagt acgagtgtac cggtcatggt gtattgaaac caagggagat aaagtgtatt
    13681 tcggctaagg catgggtggt ccatatttgt ttgccaagtc cgggccttgc ggtttgacga
    13741 tcctgcataa agtggtaagg actgccagca aaaggagtag tttagaatgg cagcggggtt
    13801 gattagtcct gcccgcgact gaattttcca ttcggagtta aatatttatt tgtcagggac
    13861 atatagaatg tcaggagggg gacaatctaa tcagcatctt cccataggaa aatttcttcc
    13921 agacatccac aaagattatc ccgagtgttt caaagaattc gtaagatcct gatttatctg
    13981 ccattcctta ccgtgaattt caaatggcaa attgaggcac aaccataata atttaaaaaa
    14041 catactaatt acggttgata ttttctaatc atgaaggagg cgttatttgt aataaaataa
    14101 atacatttat tgatgcaatg aattcaccat attaaaatca agccggatga aaattcatta
    14161 aaaatttaca ttttacacag ccaaaataaa aaaaccagtt gcgatattgt tatttttttt
    14221 caataatttg ccataaaatc aaatcaaatc acgttctttt gcgttcttgt gaataatgta
    14281 tcaaaccatg aaaaagtgcc ttttaacgac gctatttatt ggggcattcc tatatgtcgc
    14341 cgatgcacag acccaaagag tggacactgt cttttttgga ggcaagaaaa tgcttcgagt
    14401 catcaccgaa aaaggagata ctgattacct tgaccaattg agcgaagtct ccatttcctc
    14461 taaacaacag ttttccagca aggaagaata cgaccgctat atgcgatatc gtcggtatgc
    14521 tgccattgta tacccctatg ctctggaggc tatgcgtatc taccatgaat cacgagaggc
    14581 cacccgtagt atgacgaagc gcgagcgcaa gaagtatatg aaaacgctgg aaaaggaatt
    14641 ggaagatcag tttgaggagc ccctaaagaa actgacaagg acacaaggct tgatcttgac
    14701 caagatgata gagaaggagc tggatgagcc tttttacgat gttatcaaag aaataaaagg
    14761 tggctggtcg gcattctatt acaacaccac cggaaaattt tacggatatc atctgcggga
    14821 tggatatgag cgcggcaagg attatatctt agatatggtg ctggacgact atcgactaag
    14881 tgtaaaaaaa tagtgttaag tgaaaggtga aaaagcataa gtgctggatg gaagagaatg
    14941 tcttgtgtga taaaggggca cacagtaagg tgcatgaaat tcaacatcaa atgcagattt
    15001 aacctaagac acaacaattt cagatttact tgggataata ggtattcaca acttcaaata
    15061 tactcgtgaa tagaagggtt tcagtaataa tgctcccgaa gcagtaaaac ccagatggac
    15121 ggtgagcact ctggttagcc tttcagattc cggccaggtt gtatctgcaa ggtttcacag
    15181 agagtcacac tgtgtttctc aaagaatttg taataattca gattgaaata tccgcagaga
    15241 ttcaaaatgc tgccaaaacc ctcaacattg gcacatgagt aggctcaggg agaaagcacc
    15301 attaggtttt cccttcattc caataataac tatccggata gattcggagt ccgaagatag
    15361 ccgtgcccac gcgcacgatg gtggcacctt cctcgatggc cgtttccaga tcaccgctca
    15421 ttcccattga caattcattc atttccacgc cgggaaagtt aagcctgttg agttcacttc
    15481 gcagatcgct caaaaggcgg aagcagggtc tggcctgttc tggattgttg gtgaaaagac
    15541 cgatcgtcat cagccccttt attcgaagat tagagagttg agatacgctg tatacaagat
    15601 caacggcctc ttcaggagca acgccgaatt tactttcttc tgatgaagtg ttgacctgaa
    15661 tcaggacatc catagccctc cctgtctgta gcaagcgatg gtttagctta tcggcaaggt
    15721 cgaagcgatc gatggactgc acacaatcca cgttgtattt gatgatgcct ttgattttat
    15781 tggtctgcag gtggccgatg aagtgcattt catgagttat ggactttaag tcgtcatatt
    15841 tttctttgat ttcctgaacc ttattttcgc cgattagtgt ctcgccggca gcaaaagcca
    15901 actttatatt ttgtggaatg acggtctttg tggcaagaag aagccgcacc tcatccgggt
    15961 gcctgcccac tcgagtacac gcttcagcta ttctggtgcg gatggcccgg atattttcta
    16021 tgatattttc catgatatta gcttttttac ttcgtaaggg cttttaatca cctaaaattt
    16081 ttcttgatat caaaacccgg gattttatgc tcaggtttat tgacaatctc acgaaggctg
    16141 tttatttttg ttctaattaa ggaactacaa tcccctttat ttgttctaaa gaaaataaaa
    16201 tcagaaaggt tataaaatca ttgagcgaat tagtactaac cattgatggt gtcgaaccac
    16261 tcgaattact tggagaaaac aactccaaac tacagttggt caaggccaga tacccggatc
    16321 tggacatcag ttcacggggt aacaatatca agatttccgg agacaagaag ttgacacagg
    16381 aacttaagtc aaagattgaa attcttgtcc ggtatcttaa ggaaaacaaa ggattgcccg
    16441 tcacaattgt tgatacgatc ctttcgggtt ccaatccata caatgatctt gccatagagg
    16501 caaacagcaa tggcggcaat gtcatagtcc acaataagga cggatttccg attcgtgcac
    16561 ggacagtcaa ccaaaggaag ctggtagatg ccacagagcg ggcggacatc gtctttgctg
    16621 ttggtcctgc cggtaccgga aaaacctata ctgccgtagc tctggctgta aaggctctta
    16681 agaacagact ggtcaagaag atcattctga caaggcccgc ggtggaagcc ggcgagagtt
    16741 taggttttct acctggagac cttaaggaaa agatagatcc gtatcttcga ccattgtatg
    16801 atgcattaga tgatatgata ccagcggata aattagcgaa atttatgtcg gacaggatca
    16861 tcgaagtggc gccactagcc tacatgcgag gccgaacgct ggataatgcc tacatcattc
    16921 tggatgaggc gcagaacacg acttcgccac aactaaagat gtttctaaca cgactcgggc
    16981 ctaatgccaa gatcatcgtc accggtgacc tgacgcagat cgatctgcca tacagccagc
    17041 aatcagggct taagcgggca ctgtccatac tttcagatgt ggaaggtatc gcccagatct
    17101 atctcaacga agaggatgtg gtgcgtcacc gattggtcaa ggatatcatc cgtgcctata
    17161 acgtcagtga tgcagagatt cgggcaaaaa aggaaaagga gcgcacggaa agggaggccg
    17221 ccaaagaagc tacgcacagc gcaattgata aaatcaacaa cgacctgtaa aatgaaggga
    17281 atacactcaa tgtcactcat tatccttatg gtgtctacag ccatactatc caaggcacag
    17341 gagaccaagc tttataatga aagggcggac gccacccagg aaatcgccat ggctgttaca
    17401 atagcacata agaacaacca acatgtcctt atccagggtg gtggcaactg gtgttcctgg
    17461 tgtatcaagt tcaataaatt taccagggag gatgctcaga tcgattctat gataagggct
    17521 gattaccagg ttgtccatct gaattacagc aaggaaaaca agaatgaggc gatatttgct
    17581 caatacggat ttccgcagcg ctttggtttc ccggtattta tcatcctgga tggtgccggc
    17641 aatcgcatac acactcagaa cagtgaatat cttgaagatg gcaagagcgg ttatgacaga
    17701 aagcgtgtca ttgaatttct caaagcatgg tcgccagctg ctatcgaccc taaaacctat
    17761 caatccaaaa aataacgcga tatgacatca aaagttatct acaagggaca tttggaaacc
    17821 gaatgcatcc accataagtc ctcagagagc atccgcaccg atgcgcccac ggacaacaat
    17881 ggaaaaggtt cggccttcag cccaacggac ctgtgcgcca catctctggc cacgtgcatg
    17941 cttaccatca tgggtatttg ggcacgcgac aatgaggtag atatggacgg cgcctatgcg
    18001 gaagtgacta agcacatggg gacggatcca agaagaatcg tgcgtatcga cgtcaattta
    18061 cacttccctg acatgaatta ttcttccagg gttcaatctg tgttcaaaaa tatagctaag
    18121 acctgtcctg ttgcctatag tctgcatcct gacatcgagc aggtcgttac attcaactgg
    18181 taggaagatc aatctgcgat ggcaacatcc atacagcctt ctacgctcca tttcctgatc
    18241 gacctaagca tcaacaacta ccgtgtatgg tttgaggaca accgtgaggc atataaactg
    18301 gcgcgggcta attttctaga cttcatacag gaaataatag acgatctggc tcaagatgat
    18361 gattcggtcc atggattgca agccaaggat tgcgccttca gaatatatcg tgatgttcgc
    18421 ttcaacaagg acaaaagtcc gtataaagta cattttgggg cgagtatcaa gccgggggga
    18481 aggaaatcgg gcaaagcggg ttattacttt catcttgaac cgggcaagag ttttatcgga
    18541 ggaggtatct ataaccctga aagtgatcag ctttcccaca tccgaaagaa gatcagcggg
    18601 gactattccg ggttcactaa gattctggca gaagaaaaat tccggtcgtt gtacactgat
    18661 attgatcgca ccgataaata ccttctcaag cgtgttccaa gggggtttaa agccactgat
    18721 cctgccgccg gatacctcct gctgacaagt tacttgagta caattaatct ggatgacgat
    18781 ctcctcgtat ctccaacgct acacaaatat acagttgggg cctttcaggc gctacatccg
    18841 gttatccgat ttctgaatga ggtgtaagaa tgaccattag ccacctgtag agaccttcgt
    18901 cttgttgaag tcaaaagggt tgcattcatg gccaccatat acagtatatg ggccgggcac
    18961 aacaggacat cgaaaacgaa ggttgtttat tctaccggaa tatacaggtc cacgatacat
    19021 tttttttgtg gatgtttatt gaaattattt tttataattt cgaaaggtct ttgtttcccg
    19081 ggcttataac cattttcatt catccatata aatagggacg accaagcttt ttcaaaatcc
    19141 ggcagattga tttcaaagtt tcccacgatg catcgtcgcg gttcaaattc tttgaattga
    19201 ataccggaat ccggagttac tttttgatgg acagggatac caatttccat acgtacttta
    19261 ttcggagaag taattttaaa gctatcatta tagatacgaa cgaattcgac attgtcgcca
    19321 ttagtaagtt cttttagcga agcccattca aaaatgcgtg agaaggcatc atccaaaccg
    19381 tctattccga tgtggcttac aaaaaccaat ttgatggggg tgagatccat cacgtttaca
    19441 gttgcattca tatgtaacca atttattaag ttattgatgc gccaaaggta ttgatcgaat
    19501 ttttcgaaag tttgtccatt cttgctttta ataattttgt catattgaga cgagctcttt
    19561 tttttgaatt gggaagggtt gatattgtac attttcttga aggtcttagt caaggacgca
    19621 ttgcttgaaa acccatttaa ataggcgagt tgggtgatgg agatggtttt gtctcggatc
    19681 aggatagctg caatattttc gagtcgtttt ctggtaatat attcgtttag tgtttctccg
    19741 gtataggcct tgaaaagcct atggaagtga aaaacagaat aattgccaac agttgcaatg
    19801 gtagaaaggg acaggtccga gttaaggtta ttgtcgatat gggtcaacac tttatacagt
    19861 tgattcaaat ggaaatcgtt catttagaat agattgcgtc gatagttcaa ggatgctgaa
    19921 aaatgctttc ttagtttgaa acagtatttt aaacaaaaat aaaaactgtc gtgctgatca
    19981 atgggataaa attggatatt gaacagaaga ggagatgaaa gggggctctg aagtaggtag
    20041 atgattaata gccaccatgc ttgacggtat tgcctttcgg ggcggctttg ccaaaattca
    20101 gatgatttac tagggaatta aatttttcat ttgcctccaa atccttgtgt atggcggttt
    20161 ttgaattatt actttcctac ctacttcgat ttttgggg
//
LOCUS       DAOQMC010000009        19924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_945_length_19924_cov_4.707987, whole genome shotgun sequence.
ACCESSION   DAOQMC010000009 DAOQMC010000000
VERSION     DAOQMC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19924
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_19924_cov_4.707987"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PKV31_00820"
     CDS             <1..258
                     /locus_tag="PKV31_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HNB91418.1"
                     /translation="VPVSSTKSMTGHECWMAGASEAIYSTLMLNNNFIAPNINFEHPD
                     EHSAKLDIVTKATEKELNIVLSNSFGFGGTNSTLILKKFIE"
     gene            complement(451..2877)
                     /locus_tag="PKV31_00825"
     CDS             complement(451..2877)
                     /locus_tag="PKV31_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNB91419.1"
                     /translation="MHAQQFMMQGWYWDYPKTAAGKNWADSLNLKVEGLKNAGFTHLW
                     LPPHTASASGQNSNGYDPKDLYIGNTTTGFATRPSLDALLNHMVSIGLNPVADMVMNH
                     RDGGMAEDNPAVKSYISSYDINKANNGDNPFPSDRFRCLLPLGGSSGNGAGDYYFKLS
                     SASGHSRFFNKQYKLYMQTSKVGYQNLPSLNEVEPNGGGDCVEGNNNLPLGVDLFGNI
                     DALGCRTDEFHVHLDAGDFNPAGDYLEIYMSNPNGNYSDHRFYGIWRATPGADVVSQL
                     VYQTYTDFSGMPSAMGEMHWDCFKPNFTNSTGLQGDQDGMFFFYDYDQNTTCARDELF
                     AWAKWNCETLGIKGFRLDAVKHFPASFVGDLLDYLHDEGIDLDLIVGEWYGTNTGELA
                     GWVNDVLSAMDADTKAAMHPKIFDFALRESLRQACDVYGYDARNIFTSSLNAAGLSGY
                     HIVTFVNNHDFRDATGFASLVDNDIMLPYAYILTNNQLGVPCVFYPDYYGYYGNASGR
                     SIPPPPLKPYIDQLVKVHQQYIYGASTAVYLTQIGSSYSTTFNSGGPSTTVIYQLQGG
                     IAGKDVVVAINFAGEDLDVEQVINTTNLTEGTKFTDVTGLSNGPEQITLGSGKLNLKL
                     KARSYAVWVQGENPRIPLPVHVISLDARKNNKVVKINGKLTGLDATEKIILQKAIDPS
                     HFVDFQVLDYSAGDADFAFASEDNTPAFYNYYRLKLMGKDERVNYSEIKSVRFGMVTV
                     KLINTLAEAKLFLSGLDENMHFYEVMNIHGKKIISGRLYDTMIPVDALIPGVYFLRID
                     RNTVYKFVKP"
     gene            complement(3353..7348)
                     /locus_tag="PKV31_00830"
     CDS             complement(3353..7348)
                     /locus_tag="PKV31_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91420.1"
                     /translation="MKKTKFTARFRSERFFNPLMGILLFCSLFFYQERLNAQCSPQCE
                     PSNISLDADCIARVNFAMVVNSLSLAGCGPVFTLNLRATMNGPVLETVNNGTLVVDGM
                     SIGGQPYLYKGKTMVLEYVSVSTGNSCWNWHTFEDKLPPVIDCGATTIIPCYSDVNFA
                     NINVDDCSTPVQSHIISMITQNLNCDGDPLGIIRRVIRTYYATDAAGNVSDTCTDTVL
                     VQRPVLDSVTCPDNDSLRCDANYAKDAFGNPDPSVTGVPTIGLGHHPLFPNTLLGACN
                     LYTSYKDEITDFGCSIRIKRTWTITEGCCNDNHERQCVQIICLYDDVPPVLEVPDNFT
                     VSTGAYACTANVTIPAATATDNCKTQLNWVVTYPGGYKNQNGGFNVTLPIGTHNITYT
                     VNDGCVQNTVGTMQITVVDNVAPNAVCIENTVASIPNGAQYVRVYASSFDNGSWDNCS
                     DITLKVARMNPDCNGDLDPQITERDYIDFYCCDVSPTNSITVRLFVYDAAGNKSECMV
                     QIKIQDKTMPILVIPPDMWVPCEFNYNPDDLGSTFGYVLTDGSPRREDDIRTGLSGPI
                     VHTYLDGYATDNCGVDLDEDVSEDFDDCGAGTITRTFTATDPFGNTVVKKQTIHFYKT
                     PLNLSSSLFIAPRDTMIVNGTCTVDGLQPDDLAAEYSPRFTLSPVTTCFNLAYNYRDE
                     VYYGADDACFKIIRYWTIMDWCYASVYGLDAALATAVHFTQMIKVKNEVAPTFDPLAD
                     ITAISEDSDCFNEFVQVSNIAHDDCTPDNQLTYKYKIDLNYSVSGPATWDYTGSGNAI
                     ARNFPIGVHRICFYATDHCGNVGESCIILTVINRKKPTPVAHMLVTEIMPTAGTITLP
                     AWYFDAGSWANCGGPVWFSFSSNVNDTLHTFTCDDIGNNPPASIQFWVTDQYGNQDYV
                     TVQVIIQDNNGVCDDNLTFVAGNILTENSQGIPKVDVEGPSNTSTSNQGTFRFSSITL
                     GQTYTITPKSERDPLNGVETGDIIKIQNHILNKKALDGPYKLIAADVNQDNKITVNDI
                     VVMRKLILGKIDQFPSGQSWRFIDKKFQFADQTNPFKAAFPEHISVTPNGTVNNVDFY
                     GVKLGDVNNNVTLNFGDQSVETRNNETVTFSAQEQNLTAGVPSEVTFKASDFAKVGGF
                     QFALAAANGVKVINATSNDMTFNEDNFNVMSDQTTRISWNTENASVTEGSVTLTIVTD
                     RDMKLSEAISLSSTDLTAAAYDQTGAEMNVALKFTGTNADAVTVFQNRPNPFSDETRI
                     GITLPENMDVTLKVYDLSGRTIYQTTKEFAKGSNEILINSDMINASGVLFYEVSTKFG
                     TEMKKMIRLKN"
     gene            complement(7894..12486)
                     /locus_tag="PKV31_00835"
     CDS             complement(7894..12486)
                     /locus_tag="PKV31_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNB91421.1"
                     /translation="MKFLLKNEKMREGARMYNIIWFAILALFAIENNHSGLFGLGSDK
                     PAKASAVNKSSLPPESLNCIDSTNISISENGVSTLNPGYFVPPTYPNFAFLQLSIVGR
                     PAPIINCDDVGHYLTAVVHDPIANVTCSSVFKVEDKLPPVFNCVTLELPCGIDVYSTP
                     PPYTPLVSAVDNCTNPPTIEILTHTEKIFNCPSPYTMEIVRTYKATDASGNSSTCQDT
                     VRFLRPILSQIVFPDDTCFNCDAFTGDTAVTGQPTWRGYPLTGVCATWFGYSDQIIDM
                     GCPGRYKIRRTWNVMNECNGTSINHVQNIMSTDTTAPVITCPNDSILQTNFGSCVASY
                     TFPSPQATDNCSTVITFQYKVDGVIVNAPTTTLSLGSHSIEVIANDGCYNTSSCTYNV
                     TVEDKQGPSITCHDIIVSLNESGIGVVHKDSLDFDYFDNCSGPLTVKIKKSTSSTWSD
                     TVNYTCAELGGNNILQFRVCDQYGNCTICEDSVTVQDKMAPMVDCGTTPVELNCTDFA
                     DFDPADYYPVATDNCGLVTFTNTISNGMNCNFVGDYTVTYTAMDASGNQASCSRIFHF
                     NNTNPLDLSDITWPADVNYTGCNPDLVGDSISGSVILNGTFCNPIYIYSSLTDTLVNG
                     SGCAVVSKEYVVIDSCVYNLSGGTQGKFTHTQFITSTGIIPPTISVPADVTVVPDDFI
                     NCTANVSLDPATASGCSTPITITNSFNGGGADASGDYPYGTTTVVYTATDVCGLTAQG
                     STDVTVEFATNDMISCPGDSTITCLNLFDSTYLPSPTIFNTCAGYTLDTLITGTIYEC
                     DNSELTVTYTITDTHGRSDACSFNITIQGPTPLTENDITWPDSSVVIECTQWLLNGYT
                     ITAPFVDTISACFNASISYVDSVIVPSFPGSVLAIKRVWQVKDDCFYDPNTGIGIFYF
                     NQIIDVIDTTGPIVINYVDGDTINAPLYTDDCKILLDLSDLQILDCSPIVVQTHSING
                     GTPANNIDASGYYTLGVSTVNYYFEDIWGNGTNFTLYIQVLDSFPPTWPCPESLDVYI
                     NPNGLDTVYASQFSFQPFQAFDNCNYHDIYMTFDSTNLADSVLYLVCTGNVSDLSIQN
                     LKVWVFDDVGLSTSCVVDLNVLEPPGQTTNCNHLMGVVGQLAADNGHPIPGAKVSAKG
                     TVEVNQMSDAFGRYEIHDIPPGSDLSMSVEKHESAINGVNVSDIIAIRDHILAKKPLN
                     NPYKLLAADVNDDGYVSVRDLLFIRKLILGYIDQYPSGLSWRFADKSYKFPNKKNPFN
                     QPDVWQRQLHDVKGVLPVVDWVGIKLGDVNDSALFGAEVVETREVKPVNVVVTKVDES
                     ETEVTIRLIMDEIEDIQGIQFDMQYDIADLELTSLQLGDLSGITSDHFRVNEYTGSVS
                     FVWDDEAGRNGNVIATLTFRKTGQGPVGNILAINDERIEAMAIDPDGKEHKVNLVSTG
                     LWSDKENKEGRLLQNSPNPFNNSTVISVILAQSVKGKLTVYDATGRKVYTKTGEFSKG
                     RNTIEIKAGNLSGSGIYIYRFESEIFTDTKKMILTL"
     gene            13080..13319
                     /locus_tag="PKV31_00840"
     CDS             13080..13319
                     /locus_tag="PKV31_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91422.1"
                     /translation="MEKKIKLLWDFRGPDAKQIAEHYDHHLQEFIEANKLKNDITGVE
                     NISDMYSIAFMVIDEDEFEMVRNVLKPKRGTVYEG"
     gene            13748..14377
                     /locus_tag="PKV31_00845"
     CDS             13748..14377
                     /locus_tag="PKV31_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB91423.1"
                     /translation="MTIRDRFKNIFEPELLSQIESRSEIKLIPEGGIILDIGQSVRIV
                     PLLLKGVLKISRVDDSGREILLYYVHANESCAMTFTCCMQQHPSEIKATAESEVELLA
                     IPIGVMDEWLSKYPTWKSFVMRTIRNRFNELLGTIDQLAFQKLDERLVTYLKEKSRAT
                     GSTLINLSHEDIAGELASSREVISRLLKKLENDNKVLLFRNQIKILRDL"
     gene            14441..14881
                     /locus_tag="PKV31_00850"
     CDS             14441..14881
                     /locus_tag="PKV31_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB91424.1"
                     /translation="MKHEVEAEWKGKMRFDSRIGDHIVAMDVPVHSGGDNSGPSPKPF
                     ILASLAGCTGLDVVAILTKQGRKPESFRLKVSGELSKKRPVEYVAAHVLYEISGNADD
                     MEAAIDAVTASQEELCGVGHMLRHFMPITWQIIYNGNEIFNNKA"
     gene            14881..15120
                     /locus_tag="PKV31_00855"
     CDS             14881..15120
                     /locus_tag="PKV31_00855"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNB91425.1"
                     /translation="MIPFILKYKMMLIGILIGAVAGYAYYYFVGCAGGTCPISSKPLN
                     STLYGALAGAVFFSDTKSNKSNKLNTKNERQDDNH"
     gene            15095..15361
                     /locus_tag="PKV31_00860"
     CDS             15095..15361
                     /locus_tag="PKV31_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB91426.1"
                     /translation="MKDKMTIIDVRTPMEFTGGNVSGSINIPLDEIETRGEEIKQMQQ
                     PIVLCCASGNRSGYATSYLKSLGIECENGGSWFEVNFLVNNLNN"
     gene            15369..15683
                     /locus_tag="PKV31_00865"
     CDS             15369..15683
                     /locus_tag="PKV31_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB91427.1"
                     /translation="MLALLKNLFGLGPKTDYKELVKNGAQIIDVRTKSEFQSGHIKGS
                     VNIPLQMLSASLAKIKKDKPVITCCASGMRSSSAKSILKSNGYVEVYNGGGWSSLQTK
                     LR"
     gene            15683..16258
                     /locus_tag="PKV31_00870"
     CDS             15683..16258
                     /locus_tag="PKV31_00870"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNB91428.1"
                     /translation="MQDKKLYTALLLALIYLSCVNTPTPVKNTDPKEFVALGDSISTQ
                     VQAVLLKNVSEALGKGGPVYAIDYCNVNAIPLTDSMSKAYHVEIQRLSDKNRNPVNAI
                     ISDSDQKAWSKIQSDKETFAELNETGEVLYYKPIFTGMPACMKCHGSEQDIASDALQL
                     LGEKYPEDKATGYRMGDLRGMWKIKFNKTTN"
     gene            16263..16556
                     /gene="trxA"
                     /locus_tag="PKV31_00875"
     CDS             16263..16556
                     /gene="trxA"
                     /locus_tag="PKV31_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNB91429.1"
                     /translation="MTFNEIIAQDKPVLVDFFAEWCGPCKMMKPILEDLKQKVGDDVT
                     IIKVDVDKNPGAAQQYRIQGVPTLMLFKKKEIKWRQSGVVSAANLEQVIKQYQ"
     gene            16560..17324
                     /locus_tag="PKV31_00880"
     CDS             16560..17324
                     /locus_tag="PKV31_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNB91430.1"
                     /translation="MRTYKILLTLLALVMIIGCKQAPPSNESEAVNYYLTAPEFAEKL
                     KEMPDVQLIDVRTPMEYAGGHIQHAVNINIDGANFQSDISKLDKSKPVMVYCLSGARS
                     AAATRQMRADGFSQVYDMAGGMLKWRAAKLPETTEVPGQVQTPAPTSGTAMASAKNLT
                     KEQFEEMTRSSKTVLVDFYADWCAPCKKMEPYLKEIATDMSDKVHLVRINVDENQQLA
                     MELGIDAIPVLRVYKKNALSWNNMGYVDKEEVLKQL"
     gene            complement(17504..>19924)
                     /gene="metH"
                     /locus_tag="PKV31_00885"
     CDS             complement(17504..>19924)
                     /gene="metH"
                     /locus_tag="PKV31_00885"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNB91431.1"
                     /translation="IEFLNLVMSEPDIARLPIMIDSSKWEVLEAGLKCVQGRCVVNSI
                     SLKEGEAEFVQQAKLVRRYGAAAVVMAFDEVGQADTIERKVSICQRAYKILVDKVGFR
                     PEDIIFDPNIFAIATGIEEHNDYAINYIEACRLIKDTCPGVKISGGVSNISFSFRGND
                     RVREAIHASFLYYAIKAGMDMGIVNAGMMEVYEELPKDLLVLVEDVIFNRNPGATEAL
                     TAYAEQVKGESRSLEKDLEWRKAPVHERLAHSLVKGITEFIIEDTEEARKMASMPLEV
                     IEGPLMDGMNIVGDLFGSGKMFLPQVVKSARVMKQSVNYLTPFIEATKSSAGDLSKGR
                     ILLATVKGDVHDIGKNIVGVVLGCNNYEIIDLGVMVSADKILNEAIAQNVGIIGLSGL
                     ITPSLDEMIHVAREMQRRKFTIPLLIGGATTSRTHTAVKIDGQYDGPVVHVLDASRSV
                     AVASVLMSPDEKVKEEFIASIAAEYEQVRTQRANMKSEKRMLSYSDARRNKLKIDWAN
                     YTPPMPAHPGVTLFSDFPLAEIRNYIDWTPFFQSWQLTGKFPAILDDVVVGESARDLY
                     NDAQRMIDRIIEEKWIKANGIIGFWPANTVEDDLIEVYTDQSRTKILEILVCRRQQQQ
                     KAAGLPNISLADFIAPKESGVIDYIGAFAVTGGMNIEEKLHEFEASQDVYNAILLKAI
                     ADRFAEAFAECMHHLVRTKYWAYAPDENLTNEDYIRERYRGIRPAPGYPACPEHSEKG
                     KLFRMMDVTANTGIELTEFFAMVPTAAVSGWYFSHPDSKYFGVGDIPDDQKAAWKAFE
                     FHRERVGD"
BASE COUNT         5981 a         4404 c         3984 g         5555 t
ORIGIN      
        1 gtacctgtca gctctaccaa gtccatgaca ggacatgaat gctggatggc aggtgccagt
       61 gaggctattt attcaacttt gatgctcaac aataacttta ttgcaccgaa tatcaacttt
      121 gagcatcccg atgaacattc agcaaaattg gacattgtaa ccaaggcaac cgagaaggaa
      181 cttaatatcg tactcagtaa cagtttcggc tttggaggaa ctaattcaac gcttatttta
      241 aaaaagttta ttgagtagat tttctctatt tgagatggat attccgatgt tgatactgct
      301 tcttttagtt gcagtgccaa taccgaaggt acattcgtta tagcccaatt tcatgggtct
      361 atacgactgt aatctcggca ttataccaaa aatttataga ttattataaa atcatcctgg
      421 tataatatct atttttggta taaatgaaaa ttaaggcttg acaaacttgt acaccgtatt
      481 tctgtcaata cgtagaaaat aaacgcccgg tataagagca tcaacaggaa tcattgtatc
      541 gtagagtcgt cctgaaatga ttttctttcc atggatattc atcacttcat aaaaatgcat
      601 attctcatcg aggccactca ggaataattt tgcttccgca agagtattaa tcagcttgac
      661 agtcaccatt ccgaagcgta cacttttaat ctcactgtaa ttaacacgtt catctttacc
      721 catcaattta agtcggtagt aattataaaa agcaggtgta ttatcttctg atgcaaaggc
      781 aaaatctgca tcacctgcgc tataatcgag tacctgaaaa tcaacaaaat gacttggatc
      841 tattgctttc tgaagaatga tcttttctgt tgcgtccaga ccggtcagct taccattgat
      901 tttaactacc ttattattct tccgcgcatc cagtgaaata acatgtacag gtaatggtat
      961 cctgggattc tcaccttgta cccacacggc atatgacctt gcttttaatt taaggttcaa
     1021 cttgcctgag ccgagtgtga tctgctccgg cccattactc aagcctgtca catcagtaaa
     1081 tttggtacct tcggttaagt ttgttgtatt gattacctgt tccacatcca aatcctcacc
     1141 ggcaaaattg attgcaacaa cgacatcctt tcctgctatt cctccttgta actggtagat
     1201 tactgtcgtc gatggtcccc ctgaattaaa tgttgtgcta taagaagatc ctatctgagt
     1261 caaatatact gcggttgatg caccataaat atactgctgg tgtaccttga ccaactgatc
     1321 aatatatggc ttgaggggag gtggcggaat cgatcgccca cttgcatttc cataatatcc
     1381 ataataatct ggatagaaaa cacaaggaac accaagctga ttgttggtga ggatgtaggc
     1441 ataaggcagc attatatcat tgtctaccaa tgacgcaaat ccagtcgcat ctctaaaatc
     1501 atgattgttg acaaaggtca caatatgata gccggataag cctgctgcat tgagcgatga
     1561 cgtaaagata tttcgggcat cgtagccata tacatcacat gcctgccgta gtgattcccg
     1621 caaagcaaag tcgaatattt ttgggtgcat ggcggctttg gtatcggcgt ccatagcact
     1681 aaggacgtca ttgacccatc cggcaagttc acctgtattt gttccatacc attccccgac
     1741 gatcaaatca aggtcgatgc cttcatcgtg cagatagtcc agcaaatcac ccacaaaaga
     1801 agcagggaag tgtttaaccg catccagcct gaatcccttt atacccaagg tctcacaatt
     1861 ccatttagcc caggcaaata actcatcccg ggcacatgtt gtattctgat catagtcata
     1921 aaaaaagaac atgccatcct ggtcaccttg aaggccggta gagttggtaa agttaggttt
     1981 gaaacaatcc cagtgcatct cacccattgc actaggcata cctgagaaat cagtataggt
     2041 ttgatacacc aactgtgaaa ccacatcagc gcccggagtg gctctccata ttccataaaa
     2101 ccgatgatca gaatagtttc cattggggtt agacatatat atttccagat aatctcctgc
     2161 cggattaaaa tctcctgcat ccagatgtac atggaattca tccgttctgc atccaagcgc
     2221 atctatattg ccaaataaat ccactcctaa tggcaggtta ttgttgccct caacgcaatc
     2281 accgccacca ttgggctcca cttcattcaa agaaggaagg ttttgatagc ctacttttga
     2341 ggtctgcata tacaacttgt actgcttatt aaaaaaacga ctgtgccctg aagcggaact
     2401 caatttaaag taataatctc ccgctccatt accacttgat ccacccaaag gaagaaggca
     2461 acgaaatcta tccgatggaa atggattgtc cccattattt gccttattta tgtcataact
     2521 tgagatatat gacttcacag caggattatc ttcagccatt ccaccatcgc ggtgattcat
     2581 caccatatcc gctaccggat ttaagccgat tgagaccata tgatttagaa gggcatccag
     2641 cgatggtcta gtggcaaatc cggtcgtagt attcccgatg tataaatctt tcggatcata
     2701 gccattacta ttttgcccgg aggcagaggc agtatgggga ggtagccaga ggtgtgtaaa
     2761 tccggcattc ttcaacccct caactttcaa attgaggcta tctgcccagt tcttgcctgc
     2821 agctgtctta ggataatccc aataccagcc ttgcatcatg aattgttgtg catgcaaatc
     2881 tgatcctgtt aataccagta accccaacaa gcataaacgg aaaatattca gcctcatctt
     2941 ccaataatca ttgtaatatc agaacaaaac taagattttt accctaatat tttctctcaa
     3001 ataaacttca gtacaataaa atttttcttg gaggatacct tctaaatgat attccggtag
     3061 gaatgtaatt aaaacttaaa gaagtttccc tttcaacaca tttagacggt tattgcaaag
     3121 caggacagtc caggataatg aatatgaatc aattgtatat gatcttggtc tgaaaaaaat
     3181 cccgggaatt tcctaagtgg taagttcaaa gaattggagg gcaaatcttt tctgctatag
     3241 gtggcagtat ggtaacttga cagtagactc aatattatta attatgtaaa ataaaaaagc
     3301 cccgtccggt acttttaccg gaacggagct catcccaaaa acagtatatt gcttaattct
     3361 taagccttat catcttcttc atctcagtgc caaacttggt acttacttcg tagaagagta
     3421 caccacttgc attgatcata tcggagttga tcaggatttc atttgatccc ttggcaaatt
     3481 ccttagttgt ctgatagata gtacgtccac tcaagtcgta aactttcaag gtaacatcca
     3541 tattctcagg taatgtgata cctattcttg tttcatcaga gaatgggttc ggtctattct
     3601 ggaatacagt aaccgcatca gcatttgtgc ctgtaaactt cagagctaca ttcatctcag
     3661 cacctgtctg atcgtaagct gcagcagtca ggtcagtaga gctcaagctg atagcttcac
     3721 tcaacttcat gtctctgtca gtaacgatag tcaatgtaac agaaccttca gttacgcttg
     3781 cgttctcagt gttccaagat attcttgtag tctggtcgct cattacgttg aagttatctt
     3841 cgttgaaagt catatcgttg ctggttgcat tgattacttt cacaccattt gcagcagcaa
     3901 gagcaaactg gaatccgcca accttagcaa aatcgcttgc tttaaacgtt acctcagaag
     3961 gtacacctgc tgtcaaattc tgttcttgtg cagagaaagt cacagtctca ttgtttctgg
     4021 tctcaacgga ctggtcacca aagttcaatg ttacgttgtt attgacatca ccgagcttca
     4081 caccatagaa gtcaacgtta ttgacagtac cgttaggagt tacagagata tgctcaggga
     4141 atgctgcctt gaatggattg gtctggtcag caaactggaa tttcttatca ataaatctcc
     4201 agctttgtcc gcttgggaac tgatcgatct tacccaaaat cagcttacgc attacaacga
     4261 tatcatttac agtaatttta ttatcctggt ttacgtcagc agcaatcaac ttatatggac
     4321 catccagagc tttcttattc agaatatggt tctggatctt gataatatcg cctgtttcaa
     4381 caccattcag tggatctctt tcactcttag gagtgattgt gtatgtctgt cccaaggtga
     4441 tggaactaaa cctgaatgta ccttggttag atgtgcttgt attgcttgga ccctcaacat
     4501 ccaccttagg aataccctga gagttttcag tcaggatatt tccggctacg aatgtcaggt
     4561 tgtcatcaca tacgccattg ttatcctgga taatcacctg aactgttaca taatcctggt
     4621 taccatattg atcagtcacc cagaattgga ttgatgcagg aggattgttt ccaatatcat
     4681 cacaagtgaa tgtgtgcagt gtatcattga catttgagct gaatgagaac cagaccggtc
     4741 caccacagtt agcccaagac cctgcatcga aataccatgc cggcaatgtt attgtacctg
     4801 cagtaggcat tatttcggtt accaacatat gcgctacagg tgtaggcttt ttcctgttga
     4861 ttacagtaag tatgatgcaa gattcgccca catttccaca atggtcagtg gcatagaaac
     4921 atattctgtg aacaccaatt gggaagttac gtgcaatagc atttcctgaa ccggtataat
     4981 cccatgtagc aggtccggag acagagtagt tcaagtcgat tttatactta taagtcaact
     5041 gattgtcagg agtacaatcg tcgtgggcaa tattggagac ctgaacaaac tcattaaagc
     5101 agtcagaatc ttcggagatt gctgtaatat cagccaatgg gtcaaaggta ggagcaactt
     5161 cattcttcac cttaatcatt tgggtaaagt gcacagctgt tgccaatgcg gcatccaagc
     5221 catacactga agcatagcac cagtccatga tagtccagta acgaattatc ttgaagcaag
     5281 catcatcagc accataatat acctcatccc tatagttgta cgccaggtta aaacatgttg
     5341 taaccggact taatgtaaat cttggagaat attctgccgc caaatcatca ggctgaaggc
     5401 catcaacagt acaagttcca tttacaatca tggtatccct aggagctatg aataagctgc
     5461 tggacagatt taatggagtc ttatagaaat gtattgtttg tttcttgact acagtattgc
     5521 caaatggatc agtagccgta aacgtacgag taattgtacc cgctccgcaa tcatcaaaat
     5581 cttcagaaac atcttcatct aaatctacgc cacaattgtc agttgcatat ccatccaaat
     5641 aggtatgtac aatcggtcca gacagtcctg tgcggatatc atcctctctt cttggggaac
     5701 catcagtcaa cacataacca aaagtagatc caagatcatc aggattataa ttgaattcac
     5761 aaggcaccca catatcagga ggtatcacaa ggataggcat ggtcttatcc tgaatcttta
     5821 tctgaaccat acattcactc ttattaccgg cagcatcata aacaaacaat ctcacagtaa
     5881 tactattggt cggactcaca tcacagcaat agaaatcgat gtagtctctt tcagtaatct
     5941 gaggatcaag atcaccgttg cagtcaggat tcatccttgc aactttgaga gtgatatcac
     6001 tacagttgtc ccaagaacca ttgtcaaagg aagatgcata tactctaaca tattgagcac
     6061 cgttaggaat tgaagcaact gtgttctcaa tgcatacagc atttggagcc acattgtcaa
     6121 ctacggttat ctgcattgta ccaacggtat tttggacaca accatcgttg accgtatatg
     6181 tgatattatg tgtaccaatt ggcagggtta cattgaatcc accattctgg ttcttataac
     6241 cgcctggata agttaccacc caattaagct gagtcttgca attatcggta gcggttgcag
     6301 caggaatcgt tacattagct gtacatgcat aagcaccagt acttaccgtg aagttatctg
     6361 gcacttcaag gactggaggc acatcatcat acaaacaaat aatctgaaca cactgccttt
     6421 catggttgtc gttacagcaa ccctctgtaa tagtccatgt tcgcttgatt cttattgagc
     6481 aaccaaagtc agtaatctcg tccttgtaac ttgtgtaaag attacatgca cctaaaagag
     6541 tattggggaa gagaggatga tggcctagtc cgatagttgg aactccggta acagatgggt
     6601 ctggattacc aaaggcatcc ttggcataat tggcatcaca cctcaatgag tcattgtcag
     6661 gacatgttac cgaatccaga acaggccttt gaaccagtac tgtgtccgta caagtatcac
     6721 tgacattacc ggcagcatca gtcgcataat aggttctgat tactctgcgg attataccca
     6781 gtggatcacc gtcacagttc agattctgtg taatcatgga tataatatga gactgaacag
     6841 gagttgagca atcatcgaca ttgatgtttg caaagtttac atcagaatag catggtataa
     6901 tagttgtagc tccacagtca atcactggtg gcaacttgtc ttcgaacgta tgccagttcc
     6961 agcaactatt tccagtggaa acactaacgt attccagaac catagtctta cctttataca
     7021 aataaggttg acctccgatt gacataccat ccacaaccaa agtaccgttg tttactgttt
     7081 caagtacagg gccattcatg gtggctctca ggttcaatgt aaacacagga ccacatccag
     7141 ccagtgataa agaattaaca accattgcaa agttaacacg ggcaatacaa tctgcatcca
     7201 acgagatatt ggatggctca cactgcggag aacactgtgc attcaggcgc tcctggtaaa
     7261 aaaatagtga acagaaaagc aatataccca taagcgggtt aaaaaacctt tccgacctaa
     7321 aacgagctgt aaatttcgtt tttttcatga gacacttagt ttaagttttt agaattaaga
     7381 attaaaatat ggtgcatgcc acaccccagc gttctgggag cgcaatactg tagtttctta
     7441 ttataattca cacccgatgt gtatcgacac aacggatgtc attgtcgttc gatcaatttg
     7501 attaaaccac ttttataaat ggtcgaatca tgttcatcga cataacaaat catctctccg
     7561 gaaatatttc attccgaaaa aagttctaat tctaaattat ctggttggaa aaggttataa
     7621 gctcaaatgt tacatttgac cttttgtaat tatatctgat agttgtaggt tggttcggtt
     7681 cttataaatc agatatagtt acagattttg tttttacgta tttctttcaa tacatggcac
     7741 aaatatataa tatgtacgtt tgtctgaacc ataccaacat agtgctatat tatatattaa
     7801 atattcaatt catttataaa tacctgattt acaaaatata aaaaggggag aaaattctcc
     7861 ccccttttaa aatatggtct taattcaaaa aaactacagt gtaagtatca ttttcttggt
     7921 atcggtaaat atttccgact caaatcgata gatgtatatt cccgaacctg acaggttccc
     7981 ggctttgatc tcaatcgtat ttctaccttt actgaattct ccggtttttg tatatacctt
     8041 cctgccagtc gcatcatata cagtgagctt accttttaca gactgtgcca atatcactga
     8101 aattacagtg gaattattga atggattcgg gctgttttgg agcaaccttc cttctttatt
     8161 ctccttatca ctccaaaggc ctgtggaaac caggttgact ttatgttcct tgccatcagg
     8221 atctattgcc attgcctcaa ttctttcgtc gtttattgcc agaatatttc ctaccggacc
     8281 ctgaccggtt ttcctgaagg tcaaggtagc aatcacattg ccattcctgc ctgcttcatc
     8341 atcccagaca aatgaaacgg agcctgtata ttcattgact ctgaaatgat ccgaagtaat
     8401 ccccgacaag tcccctaatt gaaggctggt caattcaagg tcggcaatat catactgcat
     8461 atcgaattgg ataccctgaa tatcctctat ctcatccatt atcaatctta tggttacctc
     8521 agtttccgat tcatccactt ttgtgacaac cacattaaca ggcttaactt cacgtgtttc
     8581 aactacttcg gcaccaaaga gcgccgagtc attgacatct cccagcttga ttcccaccca
     8641 atcaacaaca ggcaatactc ccttgacatc atgcaattgc ctctgccaga catcaggctg
     8701 gttgaaagga tttttcttat ttgggaattt gtaagattta tcggcaaacc tccaactcaa
     8761 tccggatgga tactgatcga tatatccaag gatcagcttt ctgataaaca agagatccct
     8821 tacacttaca tatccatcat cattgacatc ggccgccaga agcttgtatg gattgttcag
     8881 tggctttttc gccaggatat gatccctgat agcaatgata tcgctgacat tgactccatt
     8941 aatcgcactc tcatgctttt caacactcat acttaaatca gagccgggag gaatatcatg
     9001 tatctcgtat cgtccaaagg catccgacat ctgattgact tcaacggtcc ctttggctga
     9061 tacctttgct ccgggtatag gatggccatt gtctgcagcc aattgtccca ctactcccat
     9121 tagatggtta cagttggtag tctgtccggg tggttcgagc acattcagat caacaacaca
     9181 agaagtactc aatcccacat catcgaagac ccacaccttc agattttgaa tgctgaggtc
     9241 actgacattt cctgtacaga caaggtacaa gacagaatca gccaaattcg tggaatcaaa
     9301 tgtcatatag atatcatgat agttgcaatt gtcaaaagcc tggaagggtt gaaaggaaaa
     9361 ttgactggca taaaccgtat ccaaaccatt aggattgata tacacatcta agctttcggg
     9421 acatggccaa gtaggcggga aggaatccaa tacctgaata tacagtgtaa aattggttcc
     9481 attgccccag atatcttcaa aataataatt cactgttgag acgcccaatg tataataacc
     9541 ggatgcatca atattattgg ctggtgtacc gccattgata gaatgcgtct ggacgacaat
     9601 aggcgaacaa tcaagtattt gaagatcact caagtcaaga agtattttgc aatcatccgt
     9661 atataatggt gcattgattg tatcaccgtc aacataattg attactatag gtccggtagt
     9721 atctattaca tcaatgatct gattgaagta gaatatgccg attccggtgt tgggatcata
     9781 gaaacaatca tccttgacct gccaaaccct tttaatagca aggacactac caggaaaact
     9841 aggtacaatg acggaatcca catatgaaat ggaggcattg aaacaagccg aaatggtatc
     9901 cacaaaaggt gcagtaatag tatagccatt caacaaccat tgagtacact caatgacaac
     9961 gcttgaatcc ggccaggtaa tatcattttc tgtcaacgga gtcggtccct gaatcgttat
    10021 attgaagctg catgcatcag atctcccatg cgtatccgtg atggtgtaag tgaccgtcaa
    10081 ttcagaatta tcgcattcat atatggtacc ggttatcaaa gtatccaatg tatatccggc
    10141 acaagtgttg aaaattgttg gggatggcag ataagtagag tcgaatagat tcaaacatgt
    10201 aatcgttgag tctccgggac agctgatcat atcatttgta gcaaattcaa ccgttacatc
    10261 ggtcgatccc tgagctgtca gaccacatac atcagtagca gtatagacaa cggtcgtcgt
    10321 accataagga taatctccac ttgcatccgc tccgcctcca ttgaagctgt tggtaatcgt
    10381 gatgggcgta ctacatccgg acgctgttgc aggatcaaga ctgacatttg cagtgcaatt
    10441 gataaaatca tcaggcacca cagtcacatc tgccggcaca cttattgttg gaggaataat
    10501 gcctgtcgat gtaataaatt gcgtatgtgt aaacttcccc tgtgtacctc cggaaaggtt
    10561 atatacacat gagtcaataa ccacatattc ctttgacact accgcacaac cagacccatt
    10621 gaccagagta tctgtcaggg aagaatagat ataaatcgga ttacagaaag taccattcaa
    10681 tatgacagaa cccgatatag aatcgccgac cagatccgga ttacatccgg tataattgac
    10741 atctgccggc caggtaatat cagatagatc cagcggattg gtattgttaa agtggaaaat
    10801 cctgctgcat gaagcctgat taccggaagc atccattgcc gtataagtca cggtatagtc
    10861 acccacaaaa ttacaattca taccgttgga aattgtattg gtaaatgtca ccaatccaca
    10921 gttgtctgtt gcgacaggat aatagtcagc cgggtcaaaa tccgcaaagt cggtgcaatt
    10981 caattcaacc ggcgtagttc cacaatcaac catcggcgcc attttatcct gcacagtaac
    11041 cgaatcctca catatggtgc aattgccata ctgatcacac accctgaatt gcagaatatt
    11101 gtttcctcca agttcggcac aagtatagtt gaccgtatcc gaccaggtgg atgaagtgga
    11161 cttctttatc tttacggtca atggacctga gcagttatcg aaatagtcga agtcgagaga
    11221 atccttatga actaccccaa tacccgattc attcagtgaa acaatgatat cgtgacatgt
    11281 aattgaagga ccttgtttgt cttccacggt aacattgtac gtacaggaag aagtattata
    11341 acaaccgtca ttggcaatga cttcaatcga atgactaccc agcgagagtg tggtagtcgg
    11401 tgcgttgacg atcaccccat cgaccttata ttggaatgta atcactgtag agcaattgtc
    11461 cgttgcctga ggacttggga aggtataact tgccacacag ctaccaaaat ttgtctgaag
    11521 gatggagtca ttcgggcagg tgataacagg agccgttgta tcggtggaca tgatattctg
    11581 aacatgattg atggatgtcc cattacattc attcatgaca ttccacgtac gccttatctt
    11641 ataccttccc ggacagccca tgtctatgat ctgatcagaa tagccaaacc aggtcgcaca
    11701 tacccctgta agcggatatc cgcgccatgt aggctggccg gtaacagcag tatctcctgt
    11761 gaatgcatcg cagttgaagc acgtgtcatc cggaaaaact atttgactca aaattggtct
    11821 taaaaaacga acagtatcct gacaggtaga tgaattgccg gatgcatcgg ttgccttata
    11881 agttctgacg atttccatcg tatagggtga tggacagttg aagatctttt ctgtatgagt
    11941 caaaatttcg atggtgggcg gattggtaca attgtccaca gcacttacca gaggcgtata
    12001 tggcggaggt gtcgaataca catcaatacc acatggtaat tccagtgtca cacaattaaa
    12061 gacaggtggc aacttgtctt caaccttaaa cacggaagaa catgttacat tggctattgg
    12121 atcatgaact accgcagtaa gataatgacc gacatcatcg caattgatga ttggagccgg
    12181 acgtcctaca atactcagtt gcaaaaaggc aaaatttgga taggtaggtg gcacaaaata
    12241 tcccggatta agtgtagaca cgccattctc actgatggaa atattcgtgc tgtctatgca
    12301 gttgagggat tcaggtggca agctactctt attcactgca gatgcttttg ccggtttatc
    12361 ggagcctaac ccgaaaagac ctgaatgatt gttttcaatt gcgaacaagg caaggattgc
    12421 aaaccatata atattataca tccttgctcc ctccctcatt ttttcatttt taagtaaaaa
    12481 tttcataacc gatagttgta gtttctattt tgaaaaaagg gatatagctt gtacagatta
    12541 aaaagaccaa aggatgatac ttttaccacc ccttggacaa gaaaccttat tacaaaatat
    12601 atcataccgg attcaccgga tttgaatact gtgtttggga aagagaattt acttaaaagg
    12661 cggccggccc cgcaaaagac cggcctttcg gttctattaa gttatatttt taatgatata
    12721 ttaaatattt taataaatca tattattctt ttttatcatc atttagatat tacaaaatta
    12781 tgtaatatgt tttcaacggc aataccgccg aaggattata tattatattt ttttgtaata
    12841 tattagttta aattcaatat aaaactgaaa aacaccacat ctatcacatt gataatcttt
    12901 tacttatcac aaacaagata aaagccacgt caagaaacct acaatatttt taatgctcct
    12961 ttcctaaagc tgaaagaaaa aaggcacgga taataaccga actaactcaa tagccaatcc
    13021 tattgagtcg tcagtttcga accaaaaact ctatttttga gttttaactt aaaatattca
    13081 tggaaaagaa aataaagctg ctttgggact ttaggggtcc tgatgccaaa caaatagcag
    13141 aacattacga ccatcacctt caggaattta tcgaggccaa caaactgaaa aatgatatta
    13201 ccggggtgga aaacatatcg gatatgtatt cgattgcttt catggtcatc gatgaggatg
    13261 aatttgagat ggtacgcaat gtgttgaagc ccaagcgtgg gacagtgtac gagggataaa
    13321 tatcaattgt cagcggctat tggaatatag aatgattggt ccgaaattga ttgtcagatt
    13381 aaaggcaata aataatcgat tattatccta tgagacgacc gatcaataac attatgcgac
    13441 tattatctct ccgggattag gggctttgaa aaacactttc tgcgcagagc taccgcgcgc
    13501 tacattcgta taataattct atatttaatg caattattat tgacaaatga aaaaaagaat
    13561 caaacctgac aaatgtggtc gtagaaatac ggaaataaac aagggtcatc attgtggcca
    13621 tctttattat atcattgaaa attgaggaga agaagaggca aggttttaac atgataacag
    13681 gagtatgccc tcaaaagaac aaagtacata cattaatcat aaattgacaa aaaattaaat
    13741 atcgcaaatg actatcagag atcgattcaa aaatattttc gaaccggaat tgctgtccca
    13801 aatcgaatcc cgctcagaga tcaagctgat cccggaaggt ggcatcatcc tcgacatcgg
    13861 ccaatcggta cgaatcgtac ctcttttgct caaaggcgtc cttaaaataa gccgggtgga
    13921 tgattccggc cgtgagattc tcctatacta cgtccatgcc aatgaaagtt gtgccatgac
    13981 cttcacctgc tgtatgcaac aacacccgag cgaaatcaag gcaaccgctg agagtgaggt
    14041 ggagctgctc gccatcccca tcggtgttat ggacgaatgg ctctccaaat atcccacctg
    14101 gaaaagtttt gtgatgcgta ccatccgaaa tcggttcaac gaactgctcg gcaccatcga
    14161 ccaattagct tttcagaaac tggacgaacg tctggttacc tatctcaaag aaaaatcaag
    14221 agccaccggt tccacgctga tcaacctttc ccatgaggat attgcaggcg aactcgcttc
    14281 ttcccgggaa gtcatctcac gtttgctgaa aaaactggaa aatgacaaca aagtacttct
    14341 cttccgtaac caaatcaaga tactccgcga tttgtgacat ttgtcacgca aaaggaatgt
    14401 tctacttcag acctttgcat cgcaaaaaag agatatccac atgaaacacg aagtagaagc
    14461 agaatggaag ggcaagatgc gatttgacag ccggattggt gatcatattg tagcaatgga
    14521 cgttcctgtc cattcggggg gtgataatag cggaccatct ccaaaacctt tcatactggc
    14581 atcgcttgcc ggctgcaccg ggcttgatgt tgtggccatc cttacaaagc agggacgtaa
    14641 gccggaatca ttcaggttga aagtctccgg tgaattgagc aaaaaacgtc cggtagaata
    14701 tgttgctgcc catgtattgt acgaaatatc cggcaatgcc gacgatatgg aggcggcgat
    14761 cgatgccgtc acagcctctc aagaagaact gtgtggcgta ggtcacatgc tcagacactt
    14821 tatgccaatt acatggcaga ttatttacaa tgggaacgaa atatttaaca acaaggcctg
    14881 atgataccct ttatacttaa atataagatg atgctgatcg gcatcctgat tggagcggta
    14941 gcaggatacg cctattatta tttcgtaggt tgtgccggag gaacctgtcc aatctcatca
    15001 aagccgctca acagtaccct ttatggagca ttggcaggag cggtattctt ttcagacaca
    15061 aaatcaaaca aatcaaataa actgaataca aaaaatgaaa gacaagatga caatcattga
    15121 tgtccgaaca ccaatggaat tcacaggcgg gaatgtaagc ggaagcatca acatacccct
    15181 ggatgaaatc gaaacaaggg gcgaagaaat caaacaaatg caacaaccca ttgttttatg
    15241 ttgtgccagt ggtaatcgaa gtggatatgc cacatcatac ctcaagtcct tgggtatcga
    15301 atgtgaaaac ggagggagct ggtttgaagt aaatttttta gtaaacaacc ttaataattg
    15361 ataaaaaaat gctggcgttg ttaaagaacc tgttcggctt aggaccgaag acagattaca
    15421 aggaattggt caagaacggt gctcaaatca tcgatgtacg cactaaatcg gaatttcaat
    15481 ccggtcacat caaaggctcc gtcaatattc cattacagat gttgtcagcc agtctggcta
    15541 agataaagaa ggacaaaccg gtaatcacat gctgtgcatc aggtatgaga agctcctccg
    15601 caaagagcat tctcaaatct aacggttatg tcgaagtcta taatggtgga ggttggagtt
    15661 ctctccagac aaaactgaga taatgcagga taagaagtta tatacagcct tattgcttgc
    15721 actgatctat ctcagttgtg tcaacacacc gactcctgta aagaacaccg accctaagga
    15781 attcgtggcc ctgggagaca gtatctctac tcaggttcag gcagtgttgc ttaaaaatgt
    15841 cagtgaagcc ctcggaaaag gggggcctgt gtatgctata gattactgca atgtaaatgc
    15901 gataccactg accgactcca tgtcgaaggc ctatcacgtg gaaatccaaa gattgagtga
    15961 taaaaaccgg aatcctgtca atgccattat ttcggattca gaccaaaagg cttggtcaaa
    16021 gattcaatcc gacaaggaga catttgcgga acttaatgag acaggggaag tgttgtatta
    16081 caagcccata tttaccggaa tgcctgcttg tatgaaatgt catgggagcg agcaggatat
    16141 tgcatctgat gcccttcaat tgctcggaga aaaataccct gaagacaaag ctaccggcta
    16201 ccggatgggc gatctgagag gtatgtggaa aataaaattt aacaaaacaa caaattaaca
    16261 atatgacttt taatgaaatc attgcacagg acaaacccgt cctggttgac ttcttcgcag
    16321 aatggtgcgg cccttgtaaa atgatgaagc ctatcctgga agacttgaag caaaaggtcg
    16381 gtgatgatgt taccatcatc aaggtggacg tggacaagaa tcccggtgct gcacaacaat
    16441 acaggattca gggtgtgcct accctgatgc ttttcaaaaa gaaggaaatt aaatggcgcc
    16501 agtctggagt tgtctccgct gcaaatcttg aacaagtcat caaacaatat caataaacta
    16561 tgaggactta taagatattg ttgaccttgc tggccttagt aatgataatt ggatgtaagc
    16621 aagcacctcc ctctaatgag tcggaagcag taaactatta ccttacagcc cctgaatttg
    16681 cagaaaagct taaggaaatg cccgatgtac aactgattga tgtccgtacg ccgatggaat
    16741 atgccggagg tcacatacag catgcagtga atatcaatat tgatggcgct aatttccaat
    16801 ccgatatatc taaattggac aaatccaagc ctgtgatggt gtactgtctg agtggtgcaa
    16861 gaagtgctgc cgctactcgt caaatgcgtg ccgacggatt ctctcaggta tacgatatgg
    16921 caggcggcat gctcaaatgg agagctgcaa aattgccgga aacaacggaa gtccccggtc
    16981 aggtacagac acctgcccct acttcaggca ctgcgatggc ttctgccaaa aacctgacaa
    17041 aggagcagtt tgaagagatg acacgctcca gcaagacggt actggttgac ttttatgccg
    17101 actggtgtgc tccctgtaaa aagatggaac catatctgaa ggaaattgcc actgatatgt
    17161 ccgacaaagt tcacctggta cgcatcaacg tcgatgaaaa tcagcaactg gcaatggaac
    17221 tgggtatcga tgccatccct gtgctccggg tgtataagaa aaatgcttta tcctggaaca
    17281 acatgggtta tgttgataag gaagaagtac tgaagcagtt ataagtaagt caaaaccata
    17341 gagcagtaat aaaaccgggt cggtcgtcca atgggaatac cggcccaatt ttttgcattg
    17401 aatcgattct cttaggcatg ataatttatg attgaaagta ttgtactaaa agaaaacaaa
    17461 gctcgcttac ccgaatcccc cttatcctga aaattgggcc aaatcaatcc ccgaccctct
    17521 cacgatggaa ttcaaatgcc ttccaggcag ccttctgatc atcgggaata tctcctacgc
    17581 cgaaatattt gctgtccgga tggctgaaat accagcctga cactgcagct gtgggcacca
    17641 tggcaaaaaa ttcggttaac tcaatacctg tatttgcagt gacgtccatc atcctgaaca
    17701 gtttgccttt ctcggagtgt tccgggcatg caggatagcc gggtgcagga cgaataccgc
    17761 gatatctctc gcgaatgtag tcctcattgg tgaggttttc gtccggtgca taagcccagt
    17821 atttggtcct gaccaggtga tgcatacact ctgcgaatgc ctcggcgaat ctgtctgcta
    17881 ttgcctttaa aagaatagca ttatatacgt cctgagaagc ttcgaattca tgtaatttct
    17941 cttcaatatt cattccgccg gtgaccgcaa atgcaccgat gtaatctatc acaccacttt
    18001 cctttggagc gatgaagtcc gccagtgaaa tattaggcag gccggcagcc ttttgttgct
    18061 gttgtctcct gcatacaagg atttctaaga tttttgttct tgattgatct gtatatactt
    18121 cgatcaaatc gtcttcaacg gtattagccg gccaaaagcc gatgatgcca ttggccttga
    18181 tccacttttc ctcgataatc cggtcgatca tacgctgggc atcgttatat aagtcacggg
    18241 ccgattcacc tacgaccaca tcgtccaata tcgccggaaa tttaccggtc aattgccagg
    18301 actggaaaaa gggcgtccag tcaatataat ttctgatttc agccagtgga aaatcactaa
    18361 acaaggttac tcccggatgt gccggcatag gtggtgtata atttgcccag tcaatcttta
    18421 atttattcct gcgggcatca ctataagaaa gcatccgctt ttcactcttc atattcgccc
    18481 gttgtgtgcg aacctgctca tattctgcag caatggatgc aatgaattct tcttttactt
    18541 tttcgtccgg cgacatcagt acagaagcta cagcaacact acggctggca tccagcacgt
    18601 gtaccacagg cccatcatat tgcccgtcaa tctttacagc agtatgagta cgggaagtcg
    18661 tcgcaccacc aatcaacaat ggtatggtaa atttacgacg ttgcatttcc cttgcaacgt
    18721 ggatcatctc atcgagggaa ggggtaatca gaccgctcag cccgattatt ccaacattct
    18781 gcgcaattgc ttcattaagt atcttatcag cagacaccat gacacccaga tcgataattt
    18841 catagttatt acatcccagc acgacaccga cgatattttt tccaatgtca tggacatctc
    18901 ccttgactgt cgccagcaat attcttccct ttgaaagatc gcctgcagag gacttggtag
    18961 cttcaataaa aggtgtcaga taattgacgg attgcttcat gacacgggca ctttttacca
    19021 cttgaggaag gaacattttt ccggaaccaa aaaggtcgcc gacgatattc attccgtcca
    19081 taaggggccc ttcaatgacc tccaaaggca ttgaagccat tttacgcgcc tcctcggtat
    19141 cttcaatgat aaattctgta atccccttga caaggctgtg agcaagtctt tcgtgcacag
    19201 gggccttacg ccattcaagg tctttctcca aagaacgtga ttctcctttg acctgttcgg
    19261 cataagcggt caaagcctcg gtagcacccg gattccgatt gaaaataaca tcctcaacca
    19321 ataccagtaa gtcttttggc aactcttcat atacctccat catacctgca ttgactatac
    19381 ccatatccat cccggccttg atagcataat agaggaagga agcgtgtatc gcttcccgaa
    19441 cccggtcatt accccggaag gaaaatgaaa tattgcttac accaccgctg atctttacac
    19501 ccggacatgt atccttgatc aggcgacacg cctctatgta attaatcgca taatcgttgt
    19561 gttcctctat tccggtggca atggcaaata tattcgggtc gaaaataatg tcttcaggcc
    19621 taaatccgac tttgtctact aaaatcttgt acgcacgctg acatattgaa acctttcttt
    19681 ctatggtatc agcctgacca acttcgtcaa aagccattac aacagctgca gcaccatatc
    19741 ttctaacaag ttttgcctgt tggacaaatt ccgcctctcc ttccttgagg gagatcgaat
    19801 tgaccacaca ccgaccctgg acacacttca agccggcctc caggacttcc catttggagg
    19861 aatcgatcat gatcggcaac ctggcgatat ccggctccga catgacgaga ttgagaaatt
    19921 caat
//
LOCUS       DAOQMC010000010        19520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_973_length_19520_cov_4.797688, whole genome shotgun sequence.
ACCESSION   DAOQMC010000010 DAOQMC010000000
VERSION     DAOQMC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19520
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_19520_cov_4.797688"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKV31_00890"
     CDS             <1..218
                     /locus_tag="PKV31_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91432.1"
                     /translation="TEAEKEAQISTLKNLQALNEDIAGIKLAELQKAAIENRNMFDLL
                     MDVSKYCSLGQVTHALFEVGGQYRRNM"
     gene            complement(242..2464)
                     /locus_tag="PKV31_00895"
     CDS             complement(242..2464)
                     /locus_tag="PKV31_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91433.1"
                     /translation="MTFLNKKNKEAEMLLFKIREAGFSVILTESKDDYTEFVEHFYVK
                     LLQSKALHINDNNKWKVIVIGYENDIFSETVEKMFERNNFTDRKINLAHESSLISELK
                     QDKTTLLKIYQTFHFAYKFNYLFCLDLYSYEPQVMLDQISKINHLFLDLVESKKVPIY
                     FLLGLDQEQMEIMEDHLPVLFKRNDIEFVFKLPKLKPEEIRDILDEYPIYNQSKKEIQ
                     NEIVDNIYQSDNTLLQLKNAISKFKAGIVPKKDLVIGITSSDERQAQPKKVKATRTVE
                     STQLKNIYEAELDSLYNKLGTRFQRETLCKILLKMMVVEDDGKAYANDCLYQSITENL
                     HILEKDISNALRPFVEKKIISFDPDRDDSIRILKPNAFATWPMFRQMMDKSRSYDLIF
                     EIINQMYDPSDIDLINSLLNQEQLMLINSDDTEFFVNLKASQFKELDDKVSIIRSLAI
                     EVPDGKEVASEPPKANTSPRIKINTDRESTPVPNKNLLITIKKSISESEDISANPEEQ
                     PILETEDAAVDQYIEIISDEGFSESGNQDFMDESRDNKLPDEVPESSSILDNAPEPVM
                     NDTENQEPEQVVSYHQASTMDQEIHNVESPEERKSQEVMPDEAGDQLIENIIDEGWSE
                     TEDQDFTDKFEDDNIPDEVHASSSTMDIAPEPVMNDAENKEPEQVASDHPASTVEQDI
                     HAVESQEESRSQEVMPDKPKGISPVSIKLKIGIHRQDEPESSGQKTNIRPTISIKRKE
                     "
     gene            2651..2950
                     /locus_tag="PKV31_00900"
     CDS             2651..2950
                     /locus_tag="PKV31_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HNB91434.1"
                     /translation="MLPVARISDFTICPLFTPVPFVGLVPHIGGPIIGPGMPASLIGG
                     LPAACVGDFCYCNLSVNMIASGSMKVMIKGRQLARIGDMTAHGGFILTGTVKILA"
     gene            2955..4154
                     /locus_tag="PKV31_00905"
     CDS             2955..4154
                     /locus_tag="PKV31_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNB91435.1"
                     /translation="MAKPKTFTIQHEKPWDSTFQLMSWWDSEKVRKACVMVVGAGSLG
                     NEVLKNLALMNVGKIIIIDFDRIESSNLNRSVLYRAEDAEAQSFKSEIAARKIMEINP
                     NVRAIAINADITCQVGLGLFRRCDVIIGCLDNRLARLFINRYASRFNVAWIDGAIENL
                     AGSMAVYRLGKNCYECGLSATEWQNIRYRLGCPDVASRNIKYGKIPTTPISASIIAAM
                     QVQEAIKVIHGNEGFIEYAKKFYYEGMNNIAMWVESNDRYDECESHILWQDVIEDEAL
                     SHEMTIGEFFDYLEGRFENGLKINLDTELVLEITSQESEKSYDVAVLKTELTDDFLAK
                     YRSELVENFFITRSLTCISRESGLNDYTLKSIGIPALAILSIETKDDVIYIELTKDIL
                     KYTILNN"
     gene            4162..4455
                     /locus_tag="PKV31_00910"
     CDS             4162..4455
                     /locus_tag="PKV31_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91436.1"
                     /translation="MASKANEISIMLRILPKGEEVGADLHVTTTGKEIISELISNGII
                     PKNDPEGNPYVYELISKNNNAKISEDKTLLDSGIQDGAIIYVTPKLVAGYLSK"
     gene            4459..5190
                     /locus_tag="PKV31_00915"
     CDS             4459..5190
                     /locus_tag="PKV31_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HNB91437.1"
                     /translation="MEKKEYDFDDPLFQRNFLYRRLAKEHLELQKIQSDKIGIEVENV
                     RGPLDIPDTYTIHYYIRSIVGINEDQSPKYSKHHIAEIYLPVKFPMEPPRIYMKSDLW
                     HPNIKWDGKFKGRICGNTKEYGKGYDLVQLVYRIGEILQYKNYHAENTPPFPEDSMVA
                     KWVKEYAEPNKIVDKWSETYTDYSDLTAQSDEFFEDEYQEENNPTQIFTGEIPEKKVS
                     LKISMNTEPSSDEEDSTAKKISFKK"
     gene            5198..6958
                     /locus_tag="PKV31_00920"
     CDS             5198..6958
                     /locus_tag="PKV31_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91438.1"
                     /translation="MKLWYFILVVSSLMCQKGVAQGLSDEERAWINIETFSGSNKKKI
                     ALLNDYIKKYPTGDNIERAKIWRHELQNEFQQEVIDDAISSREPQKLNYILKNFPNNK
                     RSEEVRKLIQRLETERWKSLNIRDSTAMRQYIADFPNSEHSDEIFTRLLDLEKGSSTE
                     IASVIVTQEELLAMNVRELQSFLRRYPQSPLVPECLRILDKRENHYYHVILAQVDNFP
                     EFSKLYNKFKELFPESKRLSSLTTTFDTRSREYNINKDKPGFAAWNDLDKSNLEEVLN
                     YGLKYPDSPFKKRITELSVQLEDKLYKKAVMRKSMAALLQYKKMLPNGKHIIEINRLI
                     NELKNNPKYEEYSSLASSKNIESLNKFINANKNNPLVDYALGVVNNVSDILYNLTNDE
                     AGILVQLDNFDNPYLKVSDPDNVILIDSSELKSSGYFVVNFDDVVLNSEITIFDTNGR
                     QRKIQFNNQLKVDFTQAGKILRIRIEGGVPPYVFDYKNSETKFIDQTYSNVMPGKDNF
                     FLVDLDTIENLQGGNYDLRVKSNIGGQMVMLAGIVVDVHDFRLLYFILFTLLLIGLIV
                     WVVRLYIQKSRPKTIFDEFE"
     gene            6945..7862
                     /locus_tag="PKV31_00925"
     CDS             6945..7862
                     /locus_tag="PKV31_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91439.1"
                     /translation="MSLNEIIAIASIYQRGFLIIIIGLFLVVLLIFIRIILVRLRTRK
                     KIDASKVKKFQNQDKRLIENAEVKPELNINTEPKDPMTTGIKKISFTRSQRAESFKGV
                     STEEFEQLIGSTGFVMFEMDELWTDYTIKTIYMSLDCCNELNNYLNTENFVQIKNESS
                     VIPEIGGILLGRFNPVDNSLYRISLEEFIPIESVNPDLFSLEFCTESLVKELGDVSEK
                     YPHLTVMGWFHTHPGHGLFLSVPDMTIQMGFFREPYQIAMEIDSLSKNLDTGFFCQKS
                     NGQVNNSSFEKKTWFSWHSIVELLNGQNI"
     gene            7859..9226
                     /locus_tag="PKV31_00930"
     CDS             7859..9226
                     /locus_tag="PKV31_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB91440.1"
                     /translation="MRSFELVFDPNNDNRSPNAVTLSKEFNWLANFITSQFAKYFGQD
                     EGVKDVVACDLQKDNSTYAEFTRYYNLNMEERVVLLMAMAPHVCPQLLDAFFVKNSTY
                     ARPFSEFGGLKGLHHVGFLPTGETAVFLLSLNDIADRFRIMRYFAPEHPFIKHRILNL
                     EKDGNSEPMLSGKLSISKEFLSILTEDKRFDPRFSSDFPANKLETKLAWDDLILEEYT
                     LHQVEEIIKWVRLENLILENTDLNKYINPGYRALFYGPPGTGKTLTASLIGKQVGLTV
                     YRIDLSKVVSKYIGETEKNLAAIFDQGENKNWILFFDEADALFGKRTNTTDAKDRYAN
                     QEIAYLLQRIEVYSGVTILATNLKTNLDEAFSRRFQSMIYFPLPKPDQRLRLWKTTFK
                     GLRFADNVNLRKIATSYELAGGAITNVLRFCALESFSRDEQLVYHDDIISGIKKELYK
                     DGKTM"
     gene            9275..10186
                     /locus_tag="PKV31_00935"
     CDS             9275..10186
                     /locus_tag="PKV31_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91441.1"
                     /translation="MRSMILVITILSFAVAASGQDIMKIGERTNKKIQRKVESGINRG
                     IDRTINDAEKDIRNSMKSKPQKDSGKKTGDSKTKEPGQTGVTEKYDFKPMSKSVLSFN
                     ANNMKDDNQTTESFNQVIELVQLDGFKGKWIKLLPNSAYCPIMSQAINGDWAIEMNLI
                     PQVGESNKSDINFSIALVGENDYNRLFSDSGLDAHEMDSLYNYVMVQINKPASKDELN
                     ILTKTRMSGTKPLVRKKKYIPVNSEVNRNTFAISNTGSITKVYYCNRKITSFQNKSQD
                     AMKIILFVIDDSGESALYLQDINITSK"
     gene            10292..11347
                     /locus_tag="PKV31_00940"
     CDS             10292..11347
                     /locus_tag="PKV31_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNB91442.1"
                     /translation="MIKKKILVCPLNWGLGHATRCVPIIQLLIQRGHEVHLASDGIAL
                     ELLRKEFPGLPAIQLPGYDIHYKGKNLLRTVAAQLPRILKTVILEHRFVQAYVKQNSM
                     DVILSDNRFGCYSGDCVNIFITHQLRILTPLRLMSAVAGMANRFLIRHFDRIWVPDFP
                     PPQNLSGYMSSSGSLHPVYIGPLSRLQKLSKPIIRDFIVILSGPEPARSDFEQIIISQ
                     ASLTPYRYMLVRGVEDQTPPPDYSGSGISWVNNMTTEELSYAIAESRRVISRTGYTTV
                     MDLMALGASGILVPTPGQPEQEYLAQRLKQQDRFILSSQEKFDLRFLTGQSLDDDRTF
                     EPISHNLTEKAVIESGL"
     gene            11483..12067
                     /locus_tag="PKV31_00945"
     CDS             11483..12067
                     /locus_tag="PKV31_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91443.1"
                     /translation="MNNKNSGHRIIPNINVSRASKVNIRLKVLTVFCTLLITVSGIAQ
                     NSDESHYGHQHDHEHQEGHEHHKNEIGVVVAPAYFTHTREFNFGLHLHYLRSLGESKF
                     GVGAGYERIFGDHAHHFLGVVAAYRPTGAFSLILSPGLAYEDAKHAGPQFGLHIEAVY
                     EFDIKGIHIGPVFEFSYDADDIHISPGIHIGIGF"
     gene            12216..12584
                     /locus_tag="PKV31_00950"
     CDS             12216..12584
                     /locus_tag="PKV31_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNB91444.1"
                     /translation="MSDEERMLTKAEEEIMHHFWEKGQSSVSDIIAGMDDPKPPHSSV
                     STIVRILERKGFLGHEAIGRAYEYFPLVEKNEYSSRSLNKFVNDYFDGSFSGLVSFIA
                     KEQDLNMQEFNDLMDILNKK"
     gene            12590..13879
                     /locus_tag="PKV31_00955"
     CDS             12590..13879
                     /locus_tag="PKV31_00955"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNB91445.1"
                     /translation="MVIFTFAIVAIITWSLLLIPYVLWLRQSTFFFLQRGYLLGSLVI
                     GLLNGVINILPQGKLNFGLVEMIKGDNFISDNLNSIISGITIGHGAVPETVWTTILAV
                     YYIVSCILLAILAYKVLTLYMMARGNSSTYLNGMRIVESPQRHTPFSFFHLIFLSKEN
                     RPDAESLVPIVRHESTHIKQWHSMDIILTEILFVFTWFFPVVFLFKKYLTLIHEYLAD
                     AEVLKTMDTRIYGELLLSQQMGASVSVAHHFAKSELKSRFRKMVQTPTSGRHRLIYLS
                     VLPFLLLSFIWAERIDLNAITAKVNNFNQQDDNPAISREIYITPEGQMRVVKKATGPD
                     KYIPQYPGGQEALGKFIASNLKVDGYVKTKTKDVRVRLNFDSNGKIELITYLVPVLPE
                     MELALNKVFNTMPAWIAPEIGGKPVRSEVVLPLQFNW"
     gene            complement(14183..14368)
                     /locus_tag="PKV31_00960"
     CDS             complement(14183..14368)
                     /locus_tag="PKV31_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91446.1"
                     /translation="MSNEVNIYEEIVKLNMRISCLESLLQHIIEINPYLEIPPKEEID
                     RIYSKAIQQVIDEVEAG"
     gene            complement(14461..14892)
                     /locus_tag="PKV31_00965"
     CDS             complement(14461..14892)
                     /locus_tag="PKV31_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91447.1"
                     /translation="MNKNFRLAILFLSIIMMGGYGCRHSAPDWSSPVRIYDTLCSCKN
                     SSGHTVDAGLKKMLDEKNIQAENADQINTWISANATAFVAFVDSDFKNDPVFMSELNA
                     ARDTLEAHGARLETEGAFTELFSIKVKYPGCVLAMPYLITK"
     gene            15028..15273
                     /locus_tag="PKV31_00970"
     CDS             15028..15273
                     /locus_tag="PKV31_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNB91448.1"
                     /translation="MGDNWIWTIIVSGAAGYLGSLLFKGKGSGIIFNVILGVLGGAFG
                     SWLFNKLGITGIGNFLTSVVGAFILIWLFNGMSRNKG"
     gene            15270..16412
                     /locus_tag="PKV31_00975"
     CDS             15270..16412
                     /locus_tag="PKV31_00975"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB91449.1"
                     /translation="MSELIADFSEDRKADLLKKWSLILSANPAKDEDFEGVEWSDEEE
                     TIDKALIWVYGDGGAGSSNRNFSVNDWLREVDFYFPNSVSTVLQKDAIKRYGLELLLK
                     DDAFLEHIVPDIHLAVSILKMKGLLPDVAKHKARLIVKELARKLTGLFAFQTTQAIGK
                     ALRSPIPNRRPKYNHINWVKTIYKNLETYQPAKRTIIPERLIGRQNRQKTIDTLFILV
                     DQSGSMHESLVYAAIYASIFSMIPALKTHLVLFDTEVVDLTGMEDDPVEVLFASQLGG
                     GTNIGKALAYAHKTCSNPDRTLMVLISDLDETSDEVTFMREAARVASTFNRFLTILAL
                     TKEGKGHWNVENANKFTELGIVCAASTPEQFPELCAREIVLSRSGS"
     gene            17259..18668
                     /locus_tag="PKV31_00980"
     CDS             17259..18668
                     /locus_tag="PKV31_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB91450.1"
                     /translation="MDGNLNTNNNSVPNIVSEYEAMSRNGGLSLLHEKDFIELILYFE
                     KELMLDKALEITDLAIERFNFLPDLYLKKAQILLALNNTSDAFEAVDQALLLAPSENE
                     AKLLKANCLIINKEYDQAFNLLNDIMMVGSQKDRINTMLLRARIHEELKDYTQMFNQI
                     RNVLDLEPSNELAMEKLWFYMESQRSYHQGITYLNQLLDNDPYAYMAWYNLGQAYSCI
                     GEYENSLAAYEYSFLIHPSFELGYKDCADLAFQMCRYRFALKVYLEALDNIGPDSELF
                     ACIGECYYKLKEYRLARLNYRKSNKLDPYNDEVHYYLGLCYAAEHKYDIALQHYYKAL
                     KIEDSREEYFAATAEAFYKQGDADKADYYFKKATDLGGDIPEIWLSYVKFLYEIEEFD
                     RAFDVLEEADEYTYSSELLYYKVLVLIAQDDKEEACEVLKEALSEDFDNHVILYRLNP
                     ELKKDQEITSIINYFFSED"
     gene            19276..>19520
                     /locus_tag="PKV31_00985"
     CDS             19276..>19520
                     /locus_tag="PKV31_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91451.1"
                     /translation="MRLFHFLLLASLIITGSSCKTGKKLKAAQEQYKTLSDQYVALER
                     KLIDCETAVNEADKRKRVSDKEIEELNKQIVFLRENNT"
BASE COUNT         5979 a         3577 c         4184 g         5780 t
ORIGIN      
        1 ctacggaagc cgagaaagag gctcagataa gcactctgaa gaacctgcaa gcactcaatg
       61 aagatatagc cgggatcaag cttgccgagc ttcagaaagc agctattgaa aaccggaata
      121 tgtttgacct gttgatggat gtcagcaaat actgttctct gggacaggtc acacatgcat
      181 tgtttgaagt cggagggcag tacagaagga atatgtgatg attattttct gttagtattc
      241 attactcctt tctttttata gaaatcgtcg ggcgtatatt cgtcttttgg ccggaagatt
      301 ccggctcatc ttgcctatga ataccaattt tcaattttat ggatacagga ctaattccct
      361 tgggtttgtc aggcatgact tcttgtgacc gactctcctc ctgagattca acggcatgta
      421 tatcctgctc cactgttgat gccggatgat cagacgcaac ctgctccggt tctttatttt
      481 cggcatcgtt catgaccggt tcaggcgcaa tatccatagt tgaagaagaa gcatgtactt
      541 cgtcgggaat attatcatct tcgaatttat ccgtaaaatc ttgatcctct gtttctgacc
      601 atccttcatc aatgatgttc tcaataagtt gatcaccggc ttcatcaggc atgacttctt
      661 gtgacttacg ctcctcaggg gactcaacgt tatggatttc ctgatccatt gttgatgcct
      721 gatgataaga cacaacctgc tccggttctt gattttcggt atcgttcatg accggttcag
      781 gcgcattatc caggattgaa gaagattcag gtacttcgtc gggcaactta ttatccctgg
      841 attcatccat aaaatcttga tttcccgatt ctgaaaatcc ctcgtcactt atgatctcga
      901 tatattggtc aacggctgca tcttcagttt ccaatatagg ctgttcctcc ggatttgcag
      961 aaatgtcttc cgactcagat attgatttct ttatggtgat taacagattt ttattcggta
     1021 cgggagtgct ttcacggtca gtatttattt taattctggg cgaagtattt gcctttggag
     1081 gttcagaagc aacttccttc ccgtcgggga cttcgatggc aagactccga atgatgctaa
     1141 ccttgtcgtc taattcttta aattgtgaag ccttgagatt tacgaaaaac tcagtatcat
     1201 ccgaattaat cagcatcaat tgttcctgat tgagcaagct attgattaaa tcaatgtcag
     1261 atgggtcata catttgattt attatttcaa aaatcaaatc ataagatctg gatttatcca
     1321 tcatttgtct aaacattggc caggtcgcaa aagcattcgg tttcagtatt cttatactgt
     1381 catccctgtc cggatcgaac gaaatgatct tcttttcaac aaaaggtctt agggcattcg
     1441 agatgtcttt ttcaagaatg tgaaggtttt cagtaattga ctggtacaga cagtcgttgg
     1501 cgtaggcctt tccatcatct tcgacaacca tcattttgag aagaatcttg cagagtgtct
     1561 ctcgctgaaa ccgggtaccc agtttgttat agagggaatc cagttcggct tcatatatat
     1621 tttttagttg tgtcgactca acggttctgg tagcttttac tttttttggt tgcgcttgtc
     1681 gttcatcaga agaagttatt ccgataacaa ggtctttttt gggaacgatt cctgctttga
     1741 atttgcttat tgcattttta agctggagta aagtattgtc ggattgataa atattgtcaa
     1801 cgatttcgtt ttgaatttct tttttactct ggttatagat aggatattca tccagaatgt
     1861 cccggatttc ctcgggtttg agcttaggta gtttaaaaac gaattcgata tcattccttt
     1921 tgaataaaac cggcaaatga tcttccatga tttccatttg ctcctgatcc aggcctaaaa
     1981 ggaaatatat aggcactttc ttggattcaa ccaggtctag gaacaggtga tttattttgc
     2041 tgatttgatc aagcatcacc tggggttcgt atgaatacag atcaagacaa aacagataat
     2101 tgaacttata agcaaaatga aaggtttggt agatcttcag taaggtggtt ttatcttgtt
     2161 ttagctcaga aataagactg ctttcgtggg cgagattaat ttttctgtca gtaaaattgt
     2221 tacgttcgaa cattttttcc acagtttcac tgaagatatc attttcatat cctattacta
     2281 taactttcca cttattgtta tcattgatat gcagagcctt tgattgaagt aatttgacat
     2341 aaaaatgctc gacaaactct gtataatcat cctttgattc ggtaagtata accgagaagc
     2401 ccgcttccct gattttaaag agaagcattt ctgcttcctt atttttctta tttaaaaaag
     2461 tcatcagtat aactttgaat aaataaaata tttggaatta agcatgaagt ttaaaataac
     2521 caatgggatt cttaatttaa aacaaggcaa tatatagctt ttttttaaat aattataata
     2581 aacgattgtg taatttcgat aaataaaatt aattgactat ctttactccg caccaaaatg
     2641 aatctgctct atgttgcctg tagcccgaat tagtgatttt accatttgcc cgttatttac
     2701 ccctgttcca ttcgtaggat tagtacctca tattggcggt ccgattattg gtcccggtat
     2761 gcctgcaagt ttgattggtg gactgcctgc tgcctgtgtg ggtgattttt gttattgcaa
     2821 tctttcagtc aatatgatcg cgagcgggtc catgaaggtg atgataaaag gaaggcaatt
     2881 ggcaagaatt ggagacatga cagcacatgg tgggtttata ttaaccggaa ctgttaagat
     2941 tttagcgtag tgatatggca aaacctaaga catttacaat tcaacatgaa aagccatggg
     3001 attctacttt ccaactcatg tcatggtggg atagtgagaa ggtaaggaag gcctgtgtta
     3061 tggttgtcgg agccgggtct ttgggtaatg aagtattgaa gaaccttgct ttgatgaatg
     3121 tagggaagat aatcatcatt gattttgaca gaattgagtc ttccaatttg aaccgatctg
     3181 tattgtatcg ggctgaggat gcagaggcac aaagtttcaa atccgagatt gctgcacgta
     3241 aaattatgga gattaatccc aatgtgagag ccatagccat caatgccgat atcacttgcc
     3301 aagtcggatt gggcttattc agaaggtgtg atgtaatcat tggatgtctg gacaatcgac
     3361 ttgccagatt atttattaat agatatgctt ccagattcaa tgttgcatgg attgatggag
     3421 ctatagaaaa cctggcgggc tccatggcag tatatcgact ggggaaaaac tgttacgaat
     3481 gtggtctgag tgcgacagaa tggcagaata ttcggtaccg tctcggatgt ccggatgtgg
     3541 ccagccgcaa tattaagtat ggtaaaattc ctactactcc gatttcagcg tccatcattg
     3601 cagcaatgca ggtacaagag gcgattaaag tgatacatgg caatgagggc ttcatcgaat
     3661 atgcaaagaa attttattat gaaggcatga ataacatagc tatgtgggtg gaaagcaatg
     3721 atcgttatga tgagtgtgag agccacatac tatggcaaga tgttatagag gatgaagcct
     3781 tgagccacga aatgactatc ggcgaatttt tcgattatct cgaaggacgg ttcgaaaatg
     3841 gtttgaaaat caatctggat actgaactgg ttttggaaat aacgtcacag gaaagcgaaa
     3901 aaagctacga cgtagctgtt ttgaagacgg aattgacaga tgattttctg gctaaataca
     3961 ggtctgaact cgttgagaat ttttttatta cccggagctt gacatgtata tcaagagaat
     4021 ccggcctcaa tgattacaca ttgaaatcca tcggaatacc ggcactcgct attttgagta
     4081 ttgaaaccaa ggacgatgtg atatacatag aattgacaaa agatatttta aaatatacaa
     4141 tattaaacaa ctaaataaat tatggcaagc aaggctaatg aaataagcat catgttgaga
     4201 attctcccga aaggcgagga agtaggcgct gaccttcatg ttacaaccac aggcaaggaa
     4261 attatcagcg agctaatatc aaatggcatt attcctaaaa atgatccgga aggaaatcct
     4321 tatgtatatg agttgatttc gaagaataac aatgctaaaa taagtgaaga taaaacattg
     4381 ttggattcgg gcatacagga tggggctatc atatatgtca ctcccaaatt agttgcagga
     4441 tatttaagca aataaataat ggaaaaaaaa gaatatgact ttgatgatcc tctttttcag
     4501 aggaactttt tatataggcg cctcgcaaag gaacatcttg aactccagaa aatccagtct
     4561 gacaaaatag ggattgaagt ggaaaatgtg cgtggacctt tggacattcc tgacacatat
     4621 acgatacatt attatatcag aagtattgta ggaatcaatg aagatcagtc gccaaaatat
     4681 agtaaacatc acatagctga gatttatctg ccggtcaagt tccctatgga gcctcccagg
     4741 atctatatga agtctgattt gtggcatccc aatattaaat gggatggtaa gtttaaaggc
     4801 agaatttgtg gtaatacgaa ggaatacgga aaaggttatg atcttgtaca acttgtatat
     4861 cgaatcggcg agattctgca atataaaaac tatcatgcgg aaaacacacc gcccttcccg
     4921 gaagatagca tggtagccaa atgggtgaaa gaatatgctg agcccaataa aattgttgat
     4981 aagtggtcag agacctacac agactattcg gatcttactg ctcaatcgga tgaattcttt
     5041 gaggatgagt atcaggaaga aaataatcct actcaaatct tcaccggaga gataccggaa
     5101 aagaaagttt ctcttaaaat atcaatgaat acagaaccct caagcgatga ggaggacagc
     5161 acagcgaaga agatctcttt taaaaaataa ttaagatgtg aaattatggt atttcatatt
     5221 agtagtgagc tcccttatgt gccaaaaggg agtagcccag ggtctatccg atgaggaaag
     5281 agcatggatt aacattgaga ccttttccgg aagcaataaa aagaaaattg ccctgttgaa
     5341 tgattatatc aagaaatatc caaccggaga taatattgaa agggcaaaaa tatggcgaca
     5401 tgaactccag aatgaattcc aacaggaggt tatcgatgat gcgatcagta gccgggaacc
     5461 tcaaaagttg aattatatac tcaaaaactt tccaaacaat aaaagatctg aagaagtaag
     5521 aaaacttatt caaaggctcg aaacggaacg atggaaatcc ttaaatatta gggatagtac
     5581 agcaatgagg caatacatag cagactttcc caacagtgag cactcagacg aaattttcac
     5641 cagactactt gatttggaga agggttcctc cactgagatc gcttccgtaa tagtaactca
     5701 ggaagagctt ctggcaatga atgtacgaga attgcaaagt ttcctgaggc gatatcctca
     5761 atcacccctt gtccctgaat gccttaggat tttggataaa agggaaaatc attattatca
     5821 tgtgatcctc gcccaggtgg acaattttcc tgagtttagc aagctgtata ataaattcaa
     5881 agaacttttt cctgaaagta agagactaag ctccttgact accacatttg acacccgatc
     5941 ccgggaatac aacatcaata aagacaagcc gggatttgca gcatggaacg atcttgacaa
     6001 aagtaacctg gaagaagtat tgaattatgg cttaaaatat cctgattccc cctttaagaa
     6061 gcgaattact gagttgtctg ttcaattgga ggataaattg tataaaaaag ctgtgatgcg
     6121 aaagtcaatg gcagccttat tgcaatacaa aaaaatgtta cccaatggaa aacatattat
     6181 agaaataaat aggttgataa atgagcttaa aaataaccct aaatatgagg aatattcatc
     6241 actggcttct tccaaaaaca tcgaaagcct gaataaattt atcaatgcca ataaaaacaa
     6301 cccgttggta gactatgcac tcggtgtagt caataatgta tctgatattc tctacaactt
     6361 gaccaatgat gaagcaggca tattggttca actggacaat tttgacaatc cgtatttaaa
     6421 agtatcggat cctgataatg tcatcctgat tgacagtagc gaattaaaga gctcaggata
     6481 ttttgtagtc aattttgacg atgtcgtttt aaactcagaa attactattt tcgataccaa
     6541 cggaagacaa agaaaaatac agttcaataa ccaattaaag gttgatttca ctcaggccgg
     6601 gaaaatcttg agaatcagga ttgaaggagg agttcctcca tacgtttttg attataaaaa
     6661 cagtgaaact aaattcatag accagaccta ttcaaatgtt atgcctggaa aagataactt
     6721 ttttctggtg gatctggata caatagaaaa tctacagggt gggaattacg atttgcgtgt
     6781 gaaaagtaat atcggtggcc agatggtgat gttagccgga atcgttgtgg atgtccatga
     6841 ttttcgcctc ctttatttta tattgtttac cttattgctt atcggcctga ttgtgtgggt
     6901 tgttaggttg tatatacaga aaagcagacc aaaaacaata ttcgatgagt ttgaatgaaa
     6961 ttatagcgat tgcatccata tatcagcgtg gttttttaat cattatcatc ggattgtttt
     7021 tagtcgtgct cctgatattc atcagaatta ttctcgtgag gttacgtacc agaaaaaaaa
     7081 tagatgcctc aaaggtgaaa aaatttcaaa atcaagataa acgtttaatt gaaaatgctg
     7141 aagtcaagcc ggaattgaat attaacacag agcctaagga tccaatgaca accggaatca
     7201 aaaaaataag ttttacccgt agccaaaggg cagagagttt caaaggggtg agcacagaag
     7261 agtttgagca attaattgga tctaccggtt ttgtgatgtt tgaaatggat gagctgtgga
     7321 ccgactacac aattaagaca atttatatga gcctcgattg ttgcaatgag ctcaacaatt
     7381 atttgaatac tgagaatttt gttcaaatca aaaatgaatc atcagtcatc cctgaaatag
     7441 gagggatatt gctggggcgc ttcaacccgg tagataattc attgtataga attagtctgg
     7501 aagaatttat ccccattgaa agtgtcaatc ctgacctttt tagtttggag ttctgcactg
     7561 agagccttgt caaggaattg ggagatgttt cagaaaaata cccacacctg accgtcatgg
     7621 gttggtttca tacacatccc ggtcatggac tttttctttc tgtccccgac atgacaatcc
     7681 agatgggctt tttcagggag ccttatcaaa tagcgatgga aatagacagc ctttcaaaaa
     7741 atctagatac tggatttttc tgccagaaaa gtaacggaca agtcaacaat agttcatttg
     7801 aaaagaaaac atggttctca tggcattcga ttgttgaatt acttaacgga caaaatatat
     7861 gaggagtttt gaactggttt ttgatccgaa caacgataac aggtcgccca atgcagtgac
     7921 tctgagtaag gaattcaatt ggctcgcaaa tttcattaca tcacaatttg ctaaatattt
     7981 tggtcaggat gaaggagtaa aggatgtagt tgcctgtgat ttgcaaaagg ataattccac
     8041 ttatgccgaa tttaccaggt attataacct gaatatggaa gaaagagtcg tcctcctgat
     8101 ggcaatggct ccccacgtgt gcccccagct tttggatgcg ttctttgtaa aaaattcaac
     8161 ctatgcaagg cctttttctg aatttggtgg tttaaaagga ctccatcatg ttggcttttt
     8221 gcctaccgga gaaacggcag ttttcctttt atcgcttaat gatatcgcgg atagattcag
     8281 gatcatgaga tatttcgcgc cggaacatcc atttatcaaa catcggatct tgaatttgga
     8341 aaaagatgga aactccgaac ccatgctgag tggcaaactc tcgataagca aggagtttct
     8401 tagcattcta acagaagaca aacgattcga cccacggttt tccagtgatt ttcctgcaaa
     8461 taagctggaa acaaaacttg cctgggatga tttgattctt gaagaataca cactacatca
     8521 ggttgaagaa attattaaat gggttcgtct tgaaaattta atccttgaga atacggattt
     8581 aaacaaatat atcaatccgg gttacagggc acttttctac ggaccacccg ggaccgggaa
     8641 aactctgaca gcaagcttga taggaaagca ggttggcctg actgtgtatc gaatcgatct
     8701 gtcaaaagta gtatcgaagt atattggtga aacagaaaag aatcttgcag cgatctttga
     8761 tcagggtgag aataaaaatt ggatcctgtt ttttgacgag gcggatgctc tttttggcaa
     8821 aaggacaaat accacggatg caaaggacag gtatgccaat caggagatcg cctacttact
     8881 gcagagaatc gaggtatact ctggtgtgac catcctggca accaatctaa aaaccaatct
     8941 ggacgaagca ttcagccgca ggttccaatc catgatttat tttccactac ccaaacccga
     9001 tcagcgtctg aggctttgga agacaacctt caagggactc agatttgccg acaatgtcaa
     9061 tcttaggaag atcgcgacca gttatgaatt agcaggcggt gcgattacca atgttctgag
     9121 attttgtgcc ctggaaagct tttctagaga tgaacaattg gtttatcacg atgatatcat
     9181 aagtggtatc aagaaagaac tatacaaaga cggaaagaca atgtagatgt tttagtagag
     9241 aaactggatt aataactaat aaaatattcg gattatgaga agcatgatct tggtaataac
     9301 catcttatct tttgcagtgg cggcctccgg tcaggatatc atgaaaatcg gtgagcgcac
     9361 caataaaaaa attcaaagaa aggtggagtc aggcattaat cgtgggatag accgcaccat
     9421 caatgatgcc gaaaaggata ttcgcaatag catgaaaagc aaaccccaga aagattcagg
     9481 taagaaaacc ggagatagca agacgaagga gcctggacaa accggagtaa ccgagaagta
     9541 cgatttcaag ccaatgtcca agtcagtgct gtctttcaat gccaataaca tgaaggatga
     9601 taaccagaca acagaatcct tcaatcaggt aattgaattg gtacagttgg atggattcaa
     9661 aggcaaatgg attaaactgt tacctaattc tgcttattgt ccgatcatgt cccaggcaat
     9721 taatggggat tgggctattg aaatgaacct cattccccag gttggtgaaa gtaataagtc
     9781 cgatatcaat ttcagcattg ctctggtcgg tgaaaatgat tataatcgcc tcttttccga
     9841 ttcgggattg gatgcccatg agatggatag cctttacaat tatgtgatgg ttcagatcaa
     9901 caaacccgcc tcaaaggatg aactgaatat tctgaccaag acaaggatga gtggtactaa
     9961 acctcttgtc aggaaaaaga aatatatacc tgtcaactca gaggtaaaca gaaacacctt
    10021 tgccattagt aatacagggt ccatcacaaa agtgtattat tgcaacagaa agattacatc
    10081 attccagaat aaatcgcagg atgccatgaa aataatcctt tttgtcattg atgacagtgg
    10141 tgaatctgct ttatatctcc aggatatcaa tataacttca aagtaatgaa ggatatcaat
    10201 ggtgcatagt caccgtgtta tacccattca atgcttacct ttgtgctgaa agttattcaa
    10261 taggagtaat ttttatgact cccgtattct aatgataaag aagaagatac ttgtttgtcc
    10321 gcttaattgg ggtttggggc atgcaacccg ttgtgttccc attatccagc tattgattca
    10381 gcgtgggcat gaagttcatt tggcaagtga tggcattgcc ttggaactat taagaaagga
    10441 attccccggc ttgcctgcca tccaattacc tggatacgac atccattata aggggaagaa
    10501 cctgctaaga accgtagccg cgcagttgcc ccgcatattg aagactgtga ttttggaaca
    10561 caggtttgtt caggcttatg taaagcaaaa cagcatggat gtcatcctct ctgataacag
    10621 atttggctgt tattccggtg attgtgtcaa tatcttcata acacatcaac tcaggatact
    10681 gacaccgctt cgactcatga gtgctgttgc cgggatggcc aacaggttcc tgatccgtca
    10741 ttttgatcgg atatgggtac ccgactttcc acctccccaa aacctaagtg gatatatgtc
    10801 atcatctggc agcttacatc ctgtttacat cggtccttta tcccgactgc agaaactttc
    10861 aaaaccaatt atcagggact ttatagtgat cctttccggg cctgaaccgg cgaggagtga
    10921 ttttgagcag attattataa gtcaggcatc tttaacacca taccggtata tgcttgtcag
    10981 gggggtggaa gaccagacgc ctccgccgga ttactcaggt tccggtattt cctgggttaa
    11041 taacatgact accgaagagc tatcctatgc cattgcagaa agcaggaggg tcatttctcg
    11101 gaccggttat actacggtca tggatctgat ggcattgggg gcttcaggaa ttctcgtgcc
    11161 gactcccggt cagcctgaac aggagtatct ggctcaaaga ttaaagcaac aagacagatt
    11221 cattttaagt tctcaagaaa agtttgatct taggtttctc accggacaat ctttggatga
    11281 tgataggacg tttgagccaa tcagtcataa tctaacagag aaggccgtga ttgaatcagg
    11341 attataaagg aaggaagatc attgtgatcc tacattaagg caagtattga tatttaaaca
    11401 cactttaaat ttctattaat tattttataa taaggaacag ctctcttata ttgcagcaat
    11461 atttgaatca gactcgactt aaatgaacaa taaaaattca ggacatcgca ttatcccaaa
    11521 tattaatgtt agcagggcat caaaagtgaa tataagactg aaagtattaa ctgtattttg
    11581 tacgttattg attactgtgt ccggtattgc acaaaattct gatgaatcgc actatggcca
    11641 tcagcatgat catgagcacc aagaaggtca tgaacatcat aagaatgaaa ttggggtagt
    11701 agtggcaccg gcctatttta cacatacacg tgagttcaat ttcggcttac acctccatta
    11761 tctaaggagt ttaggagaat caaaatttgg agttggtgcc ggctatgaga gaattttcgg
    11821 ggatcatgcg caccactttc tgggtgtagt ggctgcttac cggcctaccg gtgcattcag
    11881 ccttatatta tcaccgggac ttgcgtatga agatgccaag catgccgggc cacaattcgg
    11941 actgcacata gaggcagtat atgaattcga catcaaggga atccatatcg ggccggtgtt
    12001 tgaattttcc tatgatgccg atgatatcca tatcagtccg ggaatccata tcgggattgg
    12061 attctaattt tatcctcttt aactaaaaat taagttgtaa gatgaaatta ttgaccgtaa
    12121 atttacgttg tcaagttaag atgattgttt tcatgggttg aaacatgaag atggttgttg
    12181 gatagaatgt cataagttaa aaaaggacta atattatgag cgatgaggaa agaatgctga
    12241 ccaaggctga ggaagagatt atgcatcact tttgggagaa ggggcagagt tctgtatcag
    12301 atatcattgc aggtatggac gatcccaaac cgcctcattc ttcggtgtct accattgtca
    12361 ggattttgga acggaaaggt tttctgggac acgaagctat tgggcgtgcg tatgaatatt
    12421 ttccattggt ggagaagaat gagtactcaa gccgttcact caacaagttt gtcaatgatt
    12481 attttgatgg ctctttttcg ggattggtgt cattcatagc aaaagagcaa gatctgaata
    12541 tgcaggaatt caatgatttg atggatatcc ttaataaaaa gtaacggtca tggtaatctt
    12601 cacattcgcc atcgtagcca tcatcacatg gagtcttttg ttgattccct atgtgctgtg
    12661 gctgagacaa tctacttttt tcttcttgca acgcggatat cttttaggct cgttggtcat
    12721 agggttgttg aatggcgtta ttaatattct tccgcaaggc aaattgaatt ttggattagt
    12781 tgagatgatt aagggtgata acttcatttc tgataacctg aattccatca taagcggaat
    12841 aaccataggt catggcgccg tccctgagac agtatggacg accattttgg ctgtttatta
    12901 cattgtaagt tgcatcctgt tggctattct ggcttataag gtactgacac tttatatgat
    12961 ggctcgagga aattcctcca cctacctcaa tggcatgcga atcgtggagt cacctcagcg
    13021 acataccccg ttttcatttt tccatcttat ttttctcagt aaagagaaca gacctgatgc
    13081 cgaatcactt gtgccgatcg tccggcatga atccacacac attaaacaat ggcactcgat
    13141 ggatataatt ctcacagaaa ttctgtttgt gtttacatgg ttttttccgg tggtgttttt
    13201 gtttaagaaa tatttgacat taatacatga atatctggca gatgcagaag tcctcaagac
    13261 aatggatacc agaatttatg gtgagctgct gctgtctcag caaatgggtg catcggtctc
    13321 ggtagcgcat cactttgcta agtcggagtt gaaaagccgg ttcaggaaga tggttcaaac
    13381 accgacgtcg ggccggcatc ggttgattta cctctccgtc ctcccttttc tgcttttgag
    13441 ttttatatgg gcggaaagga ttgacctgaa tgcaattacg gccaaagtaa ataattttaa
    13501 tcaacaggat gacaatccag cgatatctcg ggaaatatac ataactcctg aagggcagat
    13561 gcgcgtggtg aaaaaagcaa ccgggccaga taaatacata ccccaatatc cgggcggcca
    13621 ggaagccctc ggtaaattca tagcaagcaa tctgaaagta gatggctatg tgaaaactaa
    13681 aaccaaagat gtccgggttc ggttgaattt tgattctaat ggaaagattg aacttattac
    13741 ttatttagtg cctgtcctgc ctgaaatgga attagccctc aacaaagtgt tcaataccat
    13801 gcctgcatgg atagccccgg aaataggtgg aaagccggtt cgttcggagg ttgtgctgcc
    13861 tttgcagttc aattggtaga cgggcaggta ttaaaagcag gcaaaatgag ttaagaggat
    13921 ggtaattgtc cggtagggga agctttgacc caatgtagag cctgttatag gttaagcagt
    13981 ggaaaccgat tgttttatga catattcgaa gttttagtaa acaataggcg tacattgcta
    14041 ttggagaata atcatcattg ttggaatagg ggaaacggaa tcaaaggagt ctaattttcc
    14101 ctttggtcaa tccgggaaag aatagtgcgt ttctgccctt agtttattac caattaccaa
    14161 attccggcat taagagtcga tatcagcccg cttcaacttc atcaatcact tgctggattg
    14221 ctttactgta tatcctgtcg atctcttcct tgggcggaat ttcgagatag gggttaattt
    14281 caatgatgtg ctgcaacaaa gattcgaggc aggatatccg catattgagt ttgacgattt
    14341 cttcgtagat atttacttcg tttgacatgg ctttatcctt gttatgaata tgtagacaat
    14401 ttattttaat tgaacgcaag ggagtttggt atcacctata catttcctga gtacctcttt
    14461 ttattttgta atcaggtagg gcatggccaa aacacatccg gggtatttga ccttgatgga
    14521 gaacaattca gtgaaagcac cttccgtctc aagcctggca ccatgagcct ccagcgtatc
    14581 tctcgctgca ttcaactcgc tcatgaacac agggtcattt ttgaaatccg agtcaacaaa
    14641 tgcaacaaac gctgttgcat tggccgatat ccatgtattg atctgatcgg cattctctgc
    14701 ctgaatattt ttttcgtcca gcattttttt cagtcctgca tcaacagtat gtcctgaact
    14761 attcttgcac gaacacagag tgtcgtaaat cctgacagga ctgctccaat cgggtgctga
    14821 atgtctgcaa ccatacccac ccatcataat aatcgacaaa aataatatgg cgagacgaaa
    14881 gtttttattc atataaatta atcgtatatt tgttgagtag cggcattttc cccggaatat
    14941 ttgttgggga aaagccatca atctcagctt taactcaaaa tataaagcca agatacattt
    15001 ttttgaaaca ttaactcata tattatcatg ggagataatt ggatttggac gatcatcgtc
    15061 agtggagcag caggttatct ggggagctta ttgtttaaag gcaaaggcag tggtataatc
    15121 ttcaatgtaa ttctcggagt acttgggggc gctttcggga gctggctctt caataaactg
    15181 ggaataacag ggatcggtaa ttttctgaca tctgttgtag gagcctttat tttgatctgg
    15241 ttgtttaatg gcatgtcacg gaataagggt tgagtgagtt aattgcagat ttttcggaag
    15301 accgaaaggc ggacctgctg aagaaatggt cactaatcct cagtgccaat cctgccaagg
    15361 acgaagattt tgagggtgtg gaatggtccg atgaggagga aaccatcgac aaggcactga
    15421 tatgggttta tggggatggt ggtgcgggct cgtccaacag gaactttagc gtcaatgatt
    15481 ggttgcggga ggtagacttt tattttccaa acagcgtgag tacagtattg cagaaggatg
    15541 caataaagcg atatggcctt gaattgctgc tcaaggacga cgcctttttg gaacatattg
    15601 tcccggacat tcatctggca gtttccatcc tcaagatgaa aggcctgttg cccgatgtcg
    15661 caaagcacaa agcccgactt attgtaaaag aattggccag gaagctaacc ggccttttcg
    15721 cattccaaac aacacaggcc attgggaagg ctctcaggtc gcccataccc aatcgccggc
    15781 cgaaatacaa tcatatcaac tgggtcaaga ccatatacaa gaatctggaa acttatcaac
    15841 ccgctaagcg aaccattatt cctgaaaggc ttattggtcg tcaaaaccgg cagaaaacca
    15901 tcgacacact attcattctg gtagatcagt cggggtccat gcatgaatcc ctggtctatg
    15961 ctgcaatcta cgcaagcata ttttctatga ttcccgcatt gaagacacat ctggtgctat
    16021 ttgataccga agtagttgac ctcaccggaa tggaggatga tcctgtggaa gtgctttttg
    16081 cttcacaact gggaggggga accaatatag ggaaagcatt ggcctatgcg cacaagacct
    16141 gctctaatcc ggaccgtact ttgatggtcc ttatctccga tctggatgaa acatcagatg
    16201 aagtcacctt tatgcgggaa gctgcgcgtg ttgcaagtac attcaaccga tttttgacca
    16261 tattggcgct taccaaagaa ggaaaaggtc attggaatgt cgaaaatgcg aataaattta
    16321 ctgaattggg gatagtctgt gccgcatcta cccctgagca attccccgaa ctatgcgccc
    16381 gggaaattgt attatccaga tccggatcat agcaacaaaa gcggtttagt atccagaatt
    16441 gcttctcgat tcaaaccttt gttgattact catcacaaaa gatgacattt tcgtcctgtt
    16501 cagacaggca tggcgtggtt atttctataa aaatgataac ccggtgtatt tttataaata
    16561 aagagaaatt tcatgtattt ataagagccg gaacatagga ttcattgtat taacttataa
    16621 gagcaggtat aaaaaacctc gaagtacaat atatgatttg aaagtaaaca agtattggat
    16681 agtggcatct ctttgaataa aatacattca tcatgtctgt aaagtgacta attggatttt
    16741 gcagaccaat gaagagcctt tagatgaggc cttttggcag taaggataat aattgcaaga
    16801 gcccgcattt ttgtaaggta atgcagcccc ttttatttga aatcatagaa gcataattgc
    16861 caatggaaca tgaaaagttt cggataatga gcttaaactg gattgagttt tttcacattc
    16921 ataaaaagtt ttccacaatt tatcccatta acatataaca gctttattgt tagtatctga
    16981 taatcaaata ataataccat tctttaacat tatttttccg tgtaatattt tatcatttgt
    17041 cttttaattg attgaatagc tcgtgaaaag gtatttaaaa aatagtgata cttaaatgac
    17101 tgttttaaag ttgattccat acatgcaaaa tctttgtaat gccttgtaaa atcaaggttt
    17161 acagaaaaaa atatgtgaaa aaaaagtgct cggttcaatg agattaacta tatatttacc
    17221 gccgaattga atggatccca tttaagtgaa cagattatat ggacggaaac cttaatacta
    17281 acaataattc agttcccaat atcgtcagtg agtatgaagc gatgtcaagg aatggcggcc
    17341 tgagcttact acatgaaaag gactttatag aacttatatt atatttcgag aaggagttga
    17401 tgctcgataa agctttggag ataacggatt tggcaataga aagattcaat ttccttcccg
    17461 atctctacct gaagaaggct caaatcttac tagcgctcaa caatacatca gacgcattcg
    17521 aagctgtgga tcaggcattg ttgctggcac cttcagagaa tgaggccaag ttgctcaaag
    17581 ccaactgtct tattattaat aaagaatatg atcaggcatt taatctttta aacgatatca
    17641 tgatggtagg gtctcaaaag gaccggatca ataccatgtt gctcagagct cggatccatg
    17701 aggagttgaa agattatacg cagatgttta atcagattag gaatgtactt gatcttgagc
    17761 cctccaatga attggccatg gaaaaattgt ggttttatat ggagagccag cgcagttacc
    17821 accagggaat aacctatctc aatcagttgc tggacaatga tccgtatgct tatatggctt
    17881 ggtacaatct tggacaggcg tattcctgta tcggcgaata tgaaaattct ctggcagctt
    17941 acgaatacag ctttctgatc cacccttcct ttgagctggg gtataaagat tgtgcagatt
    18001 tagccttcca aatgtgccgc tatcggtttg cgctgaaagt atatcttgag gcattggata
    18061 atattggccc ggatagcgaa cttttcgctt gcattggtga atgttattat aagctgaaag
    18121 aatatcggct tgcccgactg aactaccgca aatccaacaa actggaccca tacaatgacg
    18181 aagtgcatta ctacctgggt ctttgctatg ctgccgaaca taagtatgac atagcgctcc
    18241 agcactatta caaggcattg aagatagaag acagcaggga ggaatatttt gcggcaaccg
    18301 cagaagcctt ctataagcaa ggggacgcag ataaggccga ttattatttc aagaaagcca
    18361 ctgatcttgg cggcgatatt cccgaaatct ggctttccta tgtcaaattt ctctatgaaa
    18421 tagaagaatt tgatcgtgcg ttcgatgttt tggaagaggc tgatgaatat acttacagtt
    18481 ctgagttgct ttattacaag gtcctggtat taattgctca ggatgataaa gaggaagcct
    18541 gtgaagtatt gaaagaagcg ttgagtgagg attttgacaa tcatgtcatt ctttaccgac
    18601 tcaatcctga attgaagaag gatcaggaaa tcacctccat catcaattat tttttctctg
    18661 aagattgatt gtatcctgag ggatagtgtg tgttataact atatggagaa ttgtttgata
    18721 tagccattat atgtaattta cgctttcagg actttctacc tgttggtgat acaggatgtt
    18781 ttcaattagg gtaaaattgt agtttttgat tgagtaataa tttttgatca tcattggaca
    18841 taatttgaaa tgatctttag taaacggaaa gcgagtgccg tgaaatctga tttggccggg
    18901 tcgtcctgaa ttaggtagat gattaataac ccaaaagcgg gagtatgtgc tcaatgtaga
    18961 atagtcaaat tgtaaattga tgtcaaatgt tagtccatat tctgtgaaaa catttaaaag
    19021 gcggataaat acacagcttt agattattat tttcctacct acttcgattt ggtccacaat
    19081 tgtcgttaac aatatttgca tcccattcat ttttttgagc tattcagggt ataaaaactg
    19141 agatatatcc aattttttct gtttcactgc ctatcattca gtcatttatt gttaattttg
    19201 cggtcaaatt gaacaaaaag tagttgattt ccttttactt caattagttt acgtattctt
    19261 aaataaattc cattaatgag actattccat tttttacttt tagcaagtct gatcattacc
    19321 ggatcttctt gtaagacagg taaaaagcta aaagctgcac aagaacaata taagacattg
    19381 agtgatcaat atgttgcact ggagaggaaa ttgattgatt gtgagacagc ggtcaatgaa
    19441 gcagacaaga gaaagcgtgt ttctgacaaa gaaatcgaag aactcaataa acagatcgtc
    19501 ttcctgagag agaacaatac
//
LOCUS       DAOQMC010000011        19431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_983_length_19431_cov_4.745458, whole genome shotgun sequence.
ACCESSION   DAOQMC010000011 DAOQMC010000000
VERSION     DAOQMC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 19431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19431
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_983_length_19431_cov_4.745458"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKV31_00990"
     CDS             complement(<1..593)
                     /locus_tag="PKV31_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91452.1"
                     /translation="MGRLRISKNKMNGLTQEIKTDKQVKSKKRVTDHGEVFTNEREVN
                     AMLDLVKQETERIDSRFLEPACGNGNFLAEVLRRKLNVVDQRYSKSQIEWERYAVIAV
                     SSIYGIDILEDNAQECRERLFKIFDERYTTLFKEKCKDDCRRSIKFLLGRNVLWGDAL
                     DFTNPDTKQPIVFSEWSAVNGSMLKRRDYMFKFLVEKS"
     gene            complement(1053..2696)
                     /locus_tag="PKV31_00995"
     CDS             complement(1053..2696)
                     /locus_tag="PKV31_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB91453.1"
                     /translation="MNTQRNKIPLGIDRLLFIFLFVLSFFALNAQFKIAGNASKVSEQ
                     CYDLTPDAKSQEGYILSRQKINLREDFTISVTMNFGRQNNTTSGADGIAFVMMTDTVI
                     SSSGLGGGIGYLGTSPSLIVEFDTYQNSDYNDPADDHVALIRNGNPDHASNNTISGPF
                     SLGSNIEDLNNHNVVFSWKADIQEFTVYFDCIKVLGYVGPIAGPFLGGETSAYWGFTA
                     STGNAYNRQTVCFNTPTYVDELQDLIYCDPATVQLDGGPNGLSYKWTPESGLSNPNIA
                     RPTVTVNETTTFYLEKISTCGKITLDSMQVEIIPNTIQLDLGADTSICIGNSLQLSVQ
                     EEGANYHWTTGDTTQTITVDQGGIYSVEVDNGICKKQDSMSVSVILLPSITTSPDTII
                     CRNTSAVLSASSNDSILTWSDGSTGTTITVNEEGSYTVSASNLCGQTEKTILVSVKNC
                     EEFFIPNVFSPNHDGINDYFGPTPSEAIQLIERLAIFDRWGALLYEANNITANQETSM
                     WDGTFRGRDMNPGVYAYLIQLKMSDGKVRILKGSVTLVR"
     gene            3332..11062
                     /locus_tag="PKV31_01000"
     CDS             3332..11062
                     /locus_tag="PKV31_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB91454.1"
                     /translation="MSFFKILRFTLYFLVFTTTLNAQPWFNWSYTGPDTIVVGNNCQG
                     TLFWGGDSKIICTPVNPPDQVVISKKLSSISGGYFQGQSVQAGITVTITYEAKDNQDH
                     TEEFSFTIYFADQTPPVFSPGSLPPDITVSCNTTLPPVNLTATDNCTPTFQIQITQEV
                     NIPDNACDGVYQRTFTAKDLAGNTATYVQNITLLPDTELPVIVTEAGDYVFDCSSSDA
                     LTAFQQWIGSHGGATASDNCGISGWTTIPENPVLAAACNVPTEVIFVVSDYCGNKDST
                     AATFLIMDEIAPVLETEASDRIIYCAAGPEAMLTQWLDEYGGASANDNCLGEEFIVKT
                     YLYEGESKTIEEIKDILNSQMDGPSQTVVIGGQTYEGVRAYIPIEFRFADYCEHYVST
                     AAVFAILDTTLPIIWEPGVDTTTASCTLGNIDEDFTQWYLSGAAAGGEDDCDTIFWVG
                     QPGLAAALDSLHLSGNSCNQSVTVYFRLSDGSGNLSADGFEAVYRVKDESGPYFLVHP
                     QSLGLECAGSGSSQDSLNAWIERKGDAVIEDCNNPAWDHFEWRTGAGESGSGSFGSGP
                     YPSIANEDCSQAMVVYFYAKDDCGYISIDSAFVTISDTLAPVADFIPVDTSITCSQAV
                     PQDQPVFTDNCGLFGEVEYEESSTQDPNLLACGHYSYTITRTWTAHDFCGNSTTVSQT
                     ISVVDTLAPQPDAPFVDLALQCTDNIEDYQINFSDQCSFVQVNQSGSSTQSPDHEDCA
                     HYNYVITQSYTVSDVCFNTKTFTRSITFSDVTPPDIDNSEPLVLNCTDSAQIALVLRS
                     LASDICSDALDFEISRIDLGDTSVCSSDTYQKWLLRATDPCGNTGEDTILVNLLDGSA
                     PIITQEAGDLVLYCDDLGNLETDFRDWIDHLAGSAAEDQCGHVNAFVAVEGSYDIDNP
                     ATYPGTAPDPALADKCRDGQVAQLNIDVVFYDQCFNTAVSKARFIISDTIAPKLVSCA
                     PDKEIILAAGACNTIYNLELPAVFDACANKTSDSLMQVEVPVVSSHPGNGLTPVNDVE
                     IVFNGLHSGSSFSSLGDTLLFIDIDNADIEGSEEYFNIYDENNVLLGKTNPSATQCSS
                     SSTQLSFTQVQFEDWIRDGVIRFYARPNDPSPLDGGYAINDICGGSTIKFALNVKWNN
                     NPTLRYSYALDTLAALDVDPDNLPALNLKEGRHTITFTVKDCGDNAVFCMQEVVIRDT
                     IRPVITCGNDISVTLNGVGCDSLLTIPLPLSMTDNCGLANDFNYIVPADTASAWLTFK
                     ANPNLQNYIAQNKTYTFTNINGNVTSPVLLNLYIRGDIDSGGEYFTVYGEDGEALGTT
                     EAGGGNVLTGNCSKVSTAYFNIPAAKFNAWAVDGKITFNAVSNINFDIPPGNVLSGIN
                     PCGSVPINQDGQVDSISFMFMQLKYTTFSPPSYFATGATSIPYSELQPPYDAPKYKFN
                     AGTTYFSYTISDISGNTDTCTIKVEVRDTIAPEAVCKYATLRLHPSGIFPGKLTPEMI
                     DGGSFDNCRIDTMFVTDRLYTCADIGTQPEVTLYVQDPVGLIDSCKTQILIEKALLEP
                     GYSLGLCDKDTLRLFANMPDLNLFDQYIYQWTGPNNFSSNVQNPVIPSANSNHSGSYR
                     LQVSGFNGCGGEGVVQVFINDEINTPIISAKDTVVCDGSNIVLNTQTYSGNIKYRWYE
                     GFAPDGVFIDSTIVPQLTLQRPPGEYFFYVVVSENACVSNPSSSTSVEVVTNPVAIVD
                     DASISICEGGSIRLESPLGTDYNYQWTGPNGFKSNEQYPPVISPATLSNAGLYSLVVS
                     LQNCKSAPAQVSVTVSPRPEKPTPTSNSPVCSDSELVLTSNISLGIDTFVWKRPDGTL
                     ITTTSNPLTIDSVDQGANGLWTLTLKSGGCASLESDAIKIEVTPNTTVEIHYEGPVCE
                     GDSVRLETNDFPGAAYKWTGPNGFTSTLADPTVKAAPGTYAVTIVTAAGCVFKGATTL
                     DLRQKPQIISLTTNAVDCRFPDEIITFDYITNIPEQELSFDWRGPGGFMSTEAHPAVV
                     TAQLVNGVYSVIATSRDGCVSDTAKVTISGSVAPEKPIIQGRDRLCSGDTLTLSASNL
                     PVVGKYYWETPNGTITTTSNQLTIVNIQPSNGGAFSVIYDSGNCRSRPSDVFEVKVVK
                     TPDKPVIVGDPTICLGDSIVLQISNAPDFQYNWAGPGGITSSDKKWVIFPSQQSNAGN
                     YLLTLVNEGCVSKTSDPFALTIILPPPAPKIVDPAQEFCVSGTSQLITLCIDESTATP
                     GASYAFYNTGRPKAIAGPTNALCANITNFSQLNDGVNVLYATATLNTCTSTQGIPVQI
                     TINFPPEEFAVTGKVKVACEGKQTTLSAKRPDFSTGTWKSLTANTVIVDPSDPNTEVS
                     GLSVGKNVFVWSLDYNNCKDFSRDTMIVWVSDELKAVNDVYRTYEATGLQLDVLANDI
                     FNVPVVLEVEDGPLYGTVSIKDGNTLEYAPLPGKDYDVITYRICAAGCPQLCATAKVT
                     IDIDALNDCRVPNIITPNNDGINDFLRIPCLDSLVGNTSSITIFNEWGSQVFEALPYL
                     NNFDGKYKGSDLPPGTYFYIFDKGDRSPVQKGFLIIKR"
     gene            11138..12157
                     /locus_tag="PKV31_01005"
     CDS             11138..12157
                     /locus_tag="PKV31_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNB91455.1"
                     /translation="MKMKIGETGGGIFDLSGIKAYILNQKVLLVMVSVLLLSGTGFSQ
                     QERQYSQFMYNKLAINPAFAGALPSTTFNALYRNQWMGFEGAPTTQALSANFPFLRQR
                     VGLGANLIRSTIGISEMWTLSGMYAYRLPLGNGYISGGMEGSLRYYGVDYNSPSLRAD
                     QNLASDNALIVENTRAIVPNVGLGVYYSDKNFYVGFSVPRILRSRIDFKNDLLISREV
                     THFYLMTGYSLEVAESVSLTPQLNVKYVRNSPLTIDANMMVDFGMKYNAGISYRSGGV
                     YSSSGESIDILFGMKIVEPVFLGLAYDIQLGNLGKYSNGSIECFLRYSLPAKEDMEVQ
                     NPRFF"
     gene            12194..14023
                     /locus_tag="PKV31_01010"
     CDS             12194..14023
                     /locus_tag="PKV31_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB91456.1"
                     /translation="MRHSFMVTVGILMYSCLFAQSTDVLTDIMNNQLENYFVVNQKEI
                     NSGALEFSPAIWKDSLVFVANGRKIRGRSAAEGKKLFRIFKASISEDGTLKDDGQFSD
                     LYSEYNEGPLCFNHSGDKVYFTRNIKVVDEHDKTRLNLNIFYSERIEGQWSEPIALFV
                     PGADVNYCHPAISPDGMHLYFSSNRQGGYGNYDLYRVDNVDGSWSEPVNLGPEINSAS
                     NELFPTLLSRDILSFSSNRPGGTGGFDIYLAKIFRKTSIEVTRLPAPINSESDDMSLI
                     LTGDNSKAYFSSARQGGKGMDDIYLLQITPGTEPVVIQQTLVIVDSVRVQRLQDARCV
                     LKTLAGDVVADGVTDRNGSLQVSVTKGHQYMLEVESRNYLPYQSPLWGGENKTVRLQP
                     LPCLTVNGFALEQETNKLLRDAEIVWVNDCGEPEIKVVSDSIGRFELCVPPNCSGVII
                     GRKEEFNTVRVPVPALNENLTINVNFSMVMLSIVREPIRKGVKLILDNIYYNFNESTI
                     RPSTDRELNELAILMKQYPKMRINLIAHTDSRGDANANLTLSINRAMEAKKYLVEKGI
                     AEDRIETEGRGESQLRNRCKDGVNCTEEEHQYNRRIEVKILEI"
     gene            14073..14768
                     /gene="trmB"
                     /locus_tag="PKV31_01015"
     CDS             14073..14768
                     /gene="trmB"
                     /locus_tag="PKV31_01015"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNB91457.1"
                     /translation="MSNKNKLQRFAEIAVLPNVLENFDFHDPHVADTFGNRVDIKGHW
                     NRLMFERDLPIVLELACGKGEYTTHLAVADPEKNYIGIDIKGARIWKGATMAHDQGLK
                     NVAFLRTRIELIRSFFEKNEVSEIWITFPDPFLRDSKSNRRLTSNAFLDRYTGILVPG
                     AILHLKTDDPTLYKFSLKVLENREDYEIVEHTDDFYKDEMPTPETHLLTFYEQQHLAQ
                     GKKITYIKFRYQG"
     gene            complement(14951..15202)
                     /gene="rpsT"
                     /locus_tag="PKV31_01020"
     CDS             complement(14951..15202)
                     /gene="rpsT"
                     /locus_tag="PKV31_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNB91458.1"
                     /translation="MAHHKSSLKRIRQDAKKRIQNRYYKKSTRTMIKKLRELTDKSEA
                     TTMLPKVISMVDRLAKHNNIHKNKAANLKSSLTRHVASI"
     gene            15527..15808
                     /locus_tag="PKV31_01025"
     CDS             15527..15808
                     /locus_tag="PKV31_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB91459.1"
                     /translation="MNKGDLINKIADSAKITKTQAGNALDATIKAISSSLKKGDKVTL
                     IGFGTFSVSKRSARTGRNPQTGKEIKIAAKKVARFKPGKSLADTVASGK"
     gene            16015..16785
                     /locus_tag="PKV31_01030"
     CDS             16015..16785
                     /locus_tag="PKV31_01030"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNB91460.1"
                     /translation="MSGYIGNIYNRLSEDRAHGQKRFAVLIDPDKLRMSNLDQVLKAS
                     VDAGVDYFFIGGSLIVSDVLDQLLMSIRQATNIPTVLFPGNSYQLSYKADGILFLSLI
                     SGRNPELLIGNHVLAAPYLKFSPLEILPTGYILIDGGVNTTVSYISNTRPIPANKPDI
                     ASCTAMAGEMLGLRLIFMDAGSGALNPVPAEMISAVNNVISSPLIVGGGIRTPEAARE
                     AARAGADLIVVGNAIEKDPGLIEDIASAIHTSRTISSL"
     gene            16782..17729
                     /gene="rsgA"
                     /locus_tag="PKV31_01035"
     CDS             16782..17729
                     /gene="rsgA"
                     /locus_tag="PKV31_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNB91461.1"
                     /translation="MKGIVTKSTGSWYTVRSEQGEHLECRIVGKFRLKDFELTNPVAV
                     GDEVEYKWDEVEGKGVITRIGPRRNYIVRQSPRMKHNLHLIASNIDQAVLIVTIVQPD
                     LKPGFVDRYLLMTEPHDIPAIIVFNKSDLYDEGATALLDYFRNLYENIGYETFAVSAL
                     KHDGLEALKSRLIGRTTLIGGQSGVGKSSLVNAMFSGFDLRTGEISDYSGKGVHTTTF
                     AEMYSLEEGGSIIDTPGIKTLSFNNLTPMDVAHNFRELFKMSEHCRFGGSCTHRNEPG
                     CAVREAVDKGIINEIRYQNYQAIIDELNEQNYWERKKKM"
     gene            17776..18024
                     /locus_tag="PKV31_01040"
     CDS             17776..18024
                     /locus_tag="PKV31_01040"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNB91462.1"
                     /translation="MDWKVEDNQLRKEFRFSDFKAAMAFMQLVAFEAEFLGHHPEWTN
                     VYNTVRIRLYTHDAGNVITDRDHRLADAIDRIYTDYFA"
     gene            18097..18324
                     /locus_tag="PKV31_01045"
     CDS             18097..18324
                     /locus_tag="PKV31_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91463.1"
                     /translation="MDRSIFFSQWGGFDMAEPIMKSSVFRGVIPDVPSVLSRSMKISH
                     FLFLDVNKQRMFSESISMGVIYTFFCFRVRG"
     gene            18453..19037
                     /locus_tag="PKV31_01050"
     CDS             18453..19037
                     /locus_tag="PKV31_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNB91464.1"
                     /translation="MKRFMTRPFFISLILTLSGIIFLFSSTKNDTGVHQSIYDFNATT
                     LDGAPFHFADLKGKTILVVNTASNCGFTPQFESLEALYRKYADRGLVIVGFPCNQFGG
                     QEPGGKEETEKICFAKYDVTFPMMEKTDVNGRNAHPLFVFLKDRLPGFPGKKIKWNFT
                     KFLINAEGKPIKRFAPWDKPEKIDHYLENHIWKK"
     gene            complement(19127..>19431)
                     /locus_tag="PKV31_01055"
     CDS             complement(19127..>19431)
                     /locus_tag="PKV31_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91465.1"
                     /translation="NWRSVASECRKLVQKNASTLTTITGHLPIAPINDDFTAQMYAAQ
                     IDTTDSQALHDRLYRTFNIQIPVMAQGDQFYIRYSINGFNEQADLDKLFDALRILL"
BASE COUNT         5333 a         4056 c         4539 g         5503 t
ORIGIN      
        1 gatttctcta ccaaaaattt aaacatataa tctctgcgtt tcagcataga gccgtttaca
       61 gcactccatt ctgaaaatac gatgggctgt ttggtatcgg gattggtgaa gtccagggca
      121 tcaccccata gtacatttct gcctaaaaga aactttatgc ttcgtctgca atcgtctttg
      181 catttttctt tgaaaagggt agtgtaacgc tcatcaaaga ttttaaacag tcgttctcta
      241 cattcctgtg cattgtcttc caggatatca attccgtaaa tactcgagac agcaatcacg
      301 gcatagcgtt cccattcaat ctggcttttg ctgtatcgtt ggtcaactac attcagtttt
      361 ctgcgaagga cttccgccaa aaagtttccg ttaccacaag cgggctctaa gaaacgggaa
      421 tcaatgcgtt ctgtttcttg ttttactaaa tcgagcatag cgtttacttc acgttcgttg
      481 gtaaatactt caccgtggtc ggtaaccctt tttttggatt ttacttgttt atctgtcttt
      541 atttcttgtg tcaatccgtt cattttattc tttgaaattc tcagtcttcc catttactta
      601 ttaatgatgg cttgcctgtc agtgcattcc cccaaaccgg ctcatttggt tattttggac
      661 gctaaccggc atattgaaca aaaccaaacg tgccatatat gggtcggctt attacaccgg
      721 aacactaagt ttgggcaata ccttgagcct gtagcacaac ccaaatatac tacaaaaaag
      781 caattttaat aatatgagct tttaaccagg ttccatatga tgaaagggga agtgaaagaa
      841 gaaatttgaa cctattttat tttatgacac gactgggtaa gcaaccatct aattacatct
      901 tttttcaagt catctgtggt cataccttag tgggaaaata agtatcatga caaagtacct
      961 tctctagcca accagatgtt ctcaatgatg gattcggttg aagatgacaa tgacagcgta
     1021 tttgagaggt tgagtgatgt tcggcaatgt ctttagcgaa ccagtgtgac gctgcctttc
     1081 aatatcctga ctttaccatc tgacatcttc aattggatga gataggcata cacgccggga
     1141 ttcatgtccc tgccccgaaa agtgccgtcc cacattgatg tttcctggtt ggcagtaata
     1201 ttgtttgctt cgtacaacaa tgctccccag cgatcgaata tagccaaacg ctcaatcaac
     1261 tgtattgcct cacttggtgt aggtccgaaa taatcattga ttccgtcgtg attgggagaa
     1321 aagacatttg gaatgaaaaa ttcttcacaa tttttgacac ttaccaatat tgttttttct
     1381 gtttgcccgc aaaggttaga ggcacttacc gtatagctgc cttcttcatt gaccgtaata
     1441 gtagttcctg ttgagccgtc agaccaggtc aggatgctgt cgttggatga agcgctcaac
     1501 actgcggatg tattcctaca gatgattgtg tccggtgagg tggtgataga tggcaataat
     1561 atgacggaaa ctgacatgga atcctgcttc ttgcatatgc cattgtccac ttctacagaa
     1621 tagatgccgc cttgatctac cgtaattgtt tgagtggtat ctccggtcgt ccaatgataa
     1681 ttggctcctt cctcctgaac tgaaagctgg agggaattgc caatacagat ggaagtatcc
     1741 gcgccgagat ctaattggat ggtattggga ataatctcta cctgcatgga atccagggta
     1801 atttttccac aagtggatat tttttcgaga taaaatgttg tcgtttcatt gactgttacg
     1861 gtcggtcttg cgatgttagg gttgctcaga cctgattcag gcgtccactt gtacgacagg
     1921 ccattggggc ctccgtcgag ttgtactgtt gcaggatcgc aatagataag atcctggagt
     1981 tcatccacat aagtcggggt attgaagcaa acagtctgcc tgttgtatgc atttccggtg
     2041 gatgccgtaa atccccagta agccgatgtc tcgccaccta gaaatggccc tgcaatcggc
     2101 ccgacatatc ccaacacctt gatgcaatcg aaatataccg taaattcctg aatatcagcc
     2161 ttccaggaaa aaacaacatt gtggttatta aggtcttcaa tattgctgcc cagagaaaag
     2221 gggccggaaa tggtattgtt ggatgcgtga tccggattgc catttctgat cagggccaca
     2281 tggtcatcgg ccggatcatt gtagtcggaa ttttggtaag tgtcgaattc gacaatgagc
     2341 gacgggcttg tacccagata gcctatacca ccacccaagc ccgaggagct gatcactgta
     2401 tccgtcatca tgacaaaggc aattccatcc gcacctgagg tagtgttgtt ctgtcggccg
     2461 aaattcatag tgactgaaat ggtaaaatct tcccgcaggt tgattttttg ccgggacaaa
     2521 atatagccct cctgactttt tgcatccggc gtcaaatcgt agcattgttc agaaactttg
     2581 ctggcattgc ccgctatttt gaattgagca ttcaaggcga aaaaggatag gacaaacagg
     2641 aaaataaata gcaacctgtc tatgccaagc ggtatcttat ttctttgtgt gttcaagatg
     2701 gttatgtttt atggaatgtg gatatgcaaa tttaaaaacc ggtggattaa tggaaagttc
     2761 ggtcttccaa tcagtctgcc agtgtaatga atttttaagg caaatttaat attattattt
     2821 tatttaaata ataaatatca gatatagaat taattaagta atataaatgc aagcaataag
     2881 cagttattaa gttaaggatc ggatgttttt gtactttatt ggtgtatcaa tgtgtcaaag
     2941 tgtcccggga aaggtggcag atcagtcggc cggaagtttt ttattaaaat tcaaataaaa
     3001 aaataaaaaa tggcatttcg ttcatttcag gcatcaggtt tttgtgaaag gaagcagatc
     3061 aacgtataaa acctgaatgc ttttcctttt gtccaaatgt aattacatta tttattttga
     3121 tatggttaaa tatcgcataa aatgttgata tattttacta aatgactatt tttgccttgt
     3181 tttcatccct tcatatataa aaaatattaa atgatgttgg attagtaatt tttccggtat
     3241 caaaatttta atggtgtttt tttccggaac gtgtattgat tgatactaat ccgaagagaa
     3301 tgtcaggttt tataataagt aaaattacga tatgtcgttt tttaaaattt taaggtttac
     3361 attatatttt ctggtattta caacgaccct caacgcccag ccttggttta actggagtta
     3421 caccggtccg gatacgattg tggtaggtaa taattgtcag ggtactttat tttggggtgg
     3481 ggacagtaag atcatatgca ctccggttaa tccccctgac caggtggtga tctccaagaa
     3541 gctgagctct atcagtggag gttattttca ggggcaatct gtccaggcgg gaattacagt
     3601 taccattacc tacgaggcaa aagacaatca ggatcataca gaggagttca gttttacgat
     3661 atattttgcg gatcaaactc caccggtatt ttcaccggga tcccttccac ccgacataac
     3721 ggtatcatgc aacactacat tgccacctgt caacctgaca gccaccgata attgcacgcc
     3781 gacattccag atacagatca cgcaggaggt aaacatcccc gataatgcat gtgatggagt
     3841 atatcaacgt acatttactg caaaggattt ggccgggaat acagctacct atgtacagaa
     3901 catcacattg cttcctgata ccgaacttcc tgtcattgtc actgaagccg gggattatgt
     3961 atttgattgt tcatcgtcgg atgcgctgac tgcatttcag cagtggatag gtagtcatgg
     4021 gggtgcgaca gctagtgaca attgtggtat ttccgggtgg acgaccattc ctgaaaatcc
     4081 ggtactggct gcagcttgca atgttcccac tgaagtaatt ttcgttgtaa gcgattactg
     4141 tggaaataag gatagtactg cagctacctt cctcataatg gacgaaattg ctccggtatt
     4201 ggagacggag gcttccgacc ggatcattta ttgtgctgca gggcccgaag cgatgcttac
     4261 tcaatggctg gatgagtatg gtggtgcatc agcaaacgat aactgtcttg gggaggaatt
     4321 tattgtcaaa acatacctct atgaagggga gtcaaagacg attgaagaaa ttaaagatat
     4381 attgaacagc cagatggacg ggccttctca gactgttgtc atcggagggc agacctatga
     4441 gggagtgagg gcttatatac ccatagaatt caggtttgcc gactattgcg agcattatgt
     4501 ctcgacagca gctgtatttg caattcttga tacaacactg ccaatcatct gggagccggg
     4561 agtcgatacg acaacagctt cttgtacgct tggcaacatt gacgaggatt ttacacaatg
     4621 gtatcttagt ggggccgctg ccggggggga agatgactgc gataccatct tttgggttgg
     4681 ccagccggga cttgcggcag cacttgactc tctgcatctt tccggcaatt cctgcaatca
     4741 atcagtgacg gtatatttca ggctgtcgga tggcagtggc aacctgagtg ctgatggatt
     4801 tgaggcggta tatcgtgtta aggatgaatc aggaccttat tttctggtac atccgcagag
     4861 cctgggactg gaatgtgccg gttcgggaag cagtcaggat tcactgaatg cgtggattga
     4921 aagaaaggga gatgcggtta tagaggattg caataaccct gcctgggatc attttgaatg
     4981 gcgtaccggt gcaggagaat ccggtagcgg atcattcggt agtggcccgt acccgtccat
     5041 tgcaaacgag gattgcagtc aggctatggt tgtatatttc tatgcgaagg atgattgtgg
     5101 ttatatatcc attgatagcg cctttgtaac gataagcgat acacttgcgc ccgttgctga
     5161 ttttattcct gttgatacat ctattacttg cagtcaggca gtacctcagg accaacctgt
     5221 cttcacagat aattgcgggc tgtttggcga ggtcgaatac gaggagtcca gcacccagga
     5281 tcccaatttg cttgcttgtg gacactattc ctataccatt acccggacct ggacagcgca
     5341 tgacttttgc ggcaattcaa caaccgtgtc gcaaacaata tcagttgtgg atacccttgc
     5401 cccccaacct gatgcgcctt ttgtggatct cgcactgcag tgtaccgata atatcgaaga
     5461 ctatcagatc aacttttctg atcaatgctc ctttgtacaa gtcaatcaga gtggatcatc
     5521 gacgcaatct ccggatcatg aggattgcgc ccattataat tatgtgatca cccagtccta
     5581 tacggtaagt gatgtgtgtt tcaacactaa gacatttacc cgaagcatta cattttcaga
     5641 tgttacacca ccggatattg acaattccga gccattggtg ctgaattgta ccgacagtgc
     5701 tcaaatagcc ctggtgttaa gatcattggc cagtgatata tgttccgatg cacttgattt
     5761 tgagatttcc cgcattgatt tgggggatac atccgtttgt tcatccgata cataccagaa
     5821 gtggttgcta cgtgctacag atccatgcgg aaatacaggg gaagatacca tactggtcaa
     5881 tctgctggat gggagtgcac cgataatcac gcaggaggct ggtgatctgg tattgtactg
     5941 cgatgatttg ggtaatctcg aaacagattt cagagactgg attgatcatc tcgccggttc
     6001 tgctgcagag gatcagtgtg gacatgtaaa tgcattcgtt gctgtcgaag gaagctatga
     6061 tattgacaat cctgccacct acccgggtac ggcaccggat cctgcacttg ccgataaatg
     6121 ccgggatggt caggtcgcac aacttaatat tgatgttgtt ttctatgatc aatgtttcaa
     6181 cacagctgta tcaaaggcaa gatttatcat cagtgacacc atcgcaccca aactggtttc
     6241 ctgtgctccg gataaagaga ttatcctagc tgccggagcg tgtaatacca tctataatct
     6301 ggaacttccg gccgtttttg atgcctgtgc aaacaaaaca tctgatagtc tgatgcaggt
     6361 tgaagtccct gttgtgtcat cacatcccgg caatggcctg actccggtaa atgatgtgga
     6421 aatcgtcttc aacggactgc attccggttc ttctttctct tccttaggag acactttact
     6481 tttcatagat attgacaatg cagatatcga ggggagtgaa gaatatttca atatttatga
     6541 tgaaaacaat gtgttgttgg gcaagactaa tccatcagcg acacaatgtt cctcctcaag
     6601 tacacagctt tcattcacac aggtacaatt cgaagactgg atccgggatg gtgtaatccg
     6661 tttttatgca agaccgaatg atccgtcacc tctggatggt ggatatgcaa tcaatgacat
     6721 ttgcggggga agcacgatta agtttgccct taacgtcaaa tggaataaca acccgactct
     6781 caggtactct tatgctttgg atacattggc cgcattggac gtagatccgg ataatctccc
     6841 tgccttgaac ctgaaggaag gacgtcatac cataacattt acagtgaagg attgtggaga
     6901 taatgcagtc ttctgtatgc aggaagttgt aatcagagat acgattcgtc cggtgattac
     6961 atgcggcaat gatatttccg tgactttgaa tggggtaggc tgtgattcct tactcaccat
     7021 cccgctaccg ctttccatga ccgataattg cggccttgcc aacgacttca attatatcgt
     7081 tccggcggat acagcatcag catggttgac ctttaaggcc aatccaaatc ttcagaatta
     7141 tattgcacag aacaaaacct atacatttac caatataaat ggaaatgtga caagtcctgt
     7201 tcttctcaat ctgtacattc ggggtgatat tgacagtggt ggtgaatatt ttacggtgta
     7261 tggtgaagat ggtgaagcct tgggaacaac tgaagcggga ggtggtaatg tgcttaccgg
     7321 caactgctcc aaagtatcaa ctgcatattt caatattccg gcggccaagt tcaatgcctg
     7381 ggcggtggat ggaaagatta ccttcaatgc cgtatcaaat atcaatttcg acatacctcc
     7441 gggtaatgtt ttgagtggta tcaacccatg tgggtcggtt cccatcaacc aggatggtca
     7501 ggtggatagc attagtttta tgttcatgca gctgaaatat acgacgtttt cgccgccttc
     7561 ctattttgct acgggcgcaa catcgattcc ttacagtgaa cttcagccgc cctacgatgc
     7621 tcctaaatac aagtttaatg caggaactac gtatttctca tatacgattt cggatatttc
     7681 agggaatacg gatacatgta caatcaaggt cgaggtcagg gacactattg caccggaggc
     7741 tgtgtgtaag tacgccactc tacgtctgca tccgagcggt atcttcccgg gcaagcttac
     7801 tcctgagatg attgatggag gaagctttga caattgcagg atagatacca tgtttgttac
     7861 cgatcgacta tatacctgtg cagatatagg tacacagcct gaagtgaccc tgtatgtcca
     7921 ggatccggta ggattgattg attcgtgcaa gactcagata ctgatcgaaa aggcattgtt
     7981 ggaacccggc tacagtcttg gcctgtgtga caaggatacc ttgcggttgt ttgccaatat
     8041 gcctgatttg aacttatttg atcaatatat ctatcaatgg acaggtccca ataatttttc
     8101 gtccaacgta cagaatcctg tcatcccgag tgccaattcc aatcactccg gatcgtatag
     8161 gctgcaggtg tccggcttca acggctgtgg cggtgagggt gttgtccagg tgtttattaa
     8221 tgatgagatc aacacaccga tcatttctgc taaagataca gttgtctgtg atggtagcaa
     8281 cattgtatta aacacccaga catattcagg caatatcaaa tacagatggt atgaaggctt
     8341 tgcgccggat ggcgtgttta tcgactctac cattgtgccg caattgactc ttcagcgccc
     8401 gccgggggag tatttctttt atgttgttgt atctgaaaat gcctgtgtaa gtaatccttc
     8461 atcatcgact tcagtcgagg tagtgaccaa tccggtagcc atagttgatg acgcaagcat
     8521 cagcatttgc gaaggcggta gcataagact tgagagcccg cttggtaccg attacaatta
     8581 ccaatggaca ggcccgaatg gttttaagtc caatgaacaa tatcctccgg tcatcagtcc
     8641 cgctaccctg agcaatgccg gtttgtattc cctggtggta agtttgcaaa attgtaaatc
     8701 cgctccggca caagtttcgg tgactgtctc tccaagacct gaaaaaccga cacctacctc
     8761 caattctccg gtgtgttccg attcggaatt ggttttgact tccaatattt ccttaggaat
     8821 agacaccttt gtatggaagc ggccggatgg cactttgata acgacgacat ccaacccatt
     8881 gacaatagat tctgtcgacc agggagccaa cggtttgtgg actctgacac taaaatctgg
     8941 cggatgtgct tcgttggaat cagatgccat caaaatcgaa gttacgccaa atacgacggt
     9001 ggagattcat tacgagggtc ctgtatgcga aggggatagt gtacggttgg agacaaatga
     9061 ttttcccgga gcagcctaca aatggaccgg tccgaacgga tttacctcta ccttggcaga
     9121 tccgacggtt aaggccgctc ccggaaccta tgctgtaacc attgtgactg cggcagggtg
     9181 cgttttcaag ggagctacaa cgcttgatct gaggcaaaag ccgcaaataa tatccctgac
     9241 aaccaatgcg gttgattgca ggttcccgga tgagatcatc acattcgatt atattaccaa
     9301 tataccggag caggagttga gcttcgactg gcgcggaccc ggaggattca tgagtacaga
     9361 agctcatccg gccgtagtta ccgctcagct tgtcaacggc gtatactctg tgatagccac
     9421 ttccagggat ggatgtgttt ctgatacagc caaagtcact atctccggtt cagtggcacc
     9481 ggaaaaacca atcattcagg gcagagacag gctttgctcg ggagataccc tgaccttatc
     9541 agcaagcaat cttcctgtgg tgggcaaata ttactgggag acaccaaacg gaacaatcac
     9601 aacgacatcc aatcaactga cgattgtcaa tattcagcct tccaatggcg gtgcattttc
     9661 ggtaatctat gattcaggca actgccgatc gaggccttct gatgtctttg aggtgaaggt
     9721 tgtcaaaact ccggacaaac cggtgattgt gggtgacccg actatttgcc ttggagattc
     9781 catagtattg cagataagca atgcgccgga cttccagtat aattgggcag gccctggcgg
     9841 gataacctca agtgataaaa aatgggtaat atttccttca cagcaaagca atgccggtaa
     9901 ttacctattg acgctggtca atgaaggttg tgtatccaag acatctgatc cctttgcatt
     9961 gacgattatc ttaccaccac ctgcgccaaa gatagtagat cctgcacagg agttttgcgt
    10021 cagcggaact agccaattga ttaccctgtg tatcgatgaa tcaacggcta ctccgggtgc
    10081 gagttatgca ttttacaaca caggccgacc caaggctatt gccggaccga ccaatgcact
    10141 gtgtgccaac atcaccaact ttagtcaact caacgatggc gtcaatgtcc tttatgcaac
    10201 cgctaccctg aatacatgca catcgaccca aggcataccg gttcagatta caatcaactt
    10261 cccgccggaa gaatttgcag taacaggtaa ggtaaaggtt gcctgcgaag gcaagcagac
    10321 aacattatca gcgaagcgac cggatttctc tacgggaacc tggaagtctc tgacggcgaa
    10381 tacggtgatt gttgatcctt cagatccaaa tacagaagtg tccggattat ctgttggaaa
    10441 gaatgtattt gtatggagtc tggattacaa caactgtaag gatttctctc gggacacgat
    10501 gattgtttgg gtttctgatg aattgaaagc ggtcaatgat gtctatcgta cttatgaggc
    10561 aaccgggctt caattggatg tactggccaa tgatattttc aatgtaccgg tagtgctgga
    10621 ggtggaagac gggccacttt acggaacagt tagtatcaag gacggcaata cactggaata
    10681 tgcaccactc cccggcaaag actatgatgt gataacttac aggatctgtg cagcaggatg
    10741 tcctcagtta tgtgcgactg ctaaggtgac tattgatatt gatgctctta atgattgccg
    10801 tgttccgaat attattacac caaataatga cggaatcaat gatttccttc ggataccctg
    10861 tctagatagc ttagtgggca atacttcaag cataactatt ttcaacgagt ggggaagtca
    10921 ggttttcgaa gcgttaccct atctgaacaa tttcgacgga aagtacaaag gaagtgactt
    10981 gcctcccggg acttatttct acatctttga caagggagac aggagccctg tccagaaagg
    11041 attcttaatc attaaaaggt aaagtcacgt gatggaaaca atgaatacag aaaaaacgaa
    11101 cgtatttcca aatgatacaa aaagaaatct caatggtatg aagatgaaaa ttggagagac
    11161 cggcggtggc atattcgatc tatccggaat caaggcgtac attttaaacc aaaaggtact
    11221 gctggtgatg gtgtctgtcc ttctactgtc cggcaccgga ttcagtcagc aggaaaggca
    11281 atatagccag tttatgtaca acaagctggc tatcaatcct gcctttgcag gagccctgcc
    11341 ttccaccaca ttcaatgcct tataccgtaa tcaatggatg ggttttgaag gggcgccgac
    11401 tacacaggca ttgtcggcaa acttcccttt tctgaggcag cgggttggac tgggggccaa
    11461 cctgatcagg agtaccattg gcatctcaga aatgtggaca ttaagtggaa tgtatgcata
    11521 cagattaccc ttgggcaatg gatacatcag tggaggaatg gaaggcagcc tcagatatta
    11581 cggtgtggat tacaacagtc cgtcattacg tgctgatcag aacctggcct cagacaatgc
    11641 cctgatcgtg gaaaatacca gggctatcgt accaaatgtc ggactgggag tatattattc
    11701 cgacaagaat ttttatgtcg gattctccgt gcccagaatt ttaaggtcaa ggattgactt
    11761 taaaaacgat ttgctgatat cgagggaggt gacacatttt tacttgatga caggttattc
    11821 gttggaggtg gctgagagtg tctcgctgac accgcagctt aatgtgaagt atgtcaggaa
    11881 cagtccactg accatagatg ccaatatgat ggtcgatttt ggaatgaagt acaacgccgg
    11941 tatttcctac cgtagcggtg gagtatattc atccagcgga gaatctatcg atatcctgtt
    12001 tgggatgaag attgttgagc ctgtatttct gggtctggca tacgatattc aactgggcaa
    12061 tctgggtaaa tattccaatg gaagtatcga atgttttctg aggtatagct tgccggccaa
    12121 ggaggatatg gaagttcaaa atccaaggtt tttctgataa atgatgattt ttagattgag
    12181 aatgggatct tgcatgagac acagtttcat ggtaacagtc gggatattga tgtattcgtg
    12241 cttgtttgct cagagtacgg atgttttgac tgacattatg aataatcaac tggaaaatta
    12301 ttttgtcgtc aatcaaaagg agattaattc aggtgcactg gaattttcgc ccgccatttg
    12361 gaaggatagt ctggtatttg ttgccaatgg aaggaagatt cggggaagat cagccgctga
    12421 gggaaaaaag ctattcagga tattcaaagc atctataagc gaggatggga cattgaagga
    12481 cgatggccag ttttccgatt tatattccga gtataatgag ggccctctct gctttaatca
    12541 ttcgggagat aaggtctact ttacaagaaa tattaaggtt gttgatgagc atgacaaaac
    12601 ccgattaaat ctcaatattt tttattctga aagaattgaa ggccaatgga gtgagcctat
    12661 agcattattt gtgcctggcg ccgatgtcaa ttattgtcat cctgctatca gtccggatgg
    12721 catgcatctg tatttctctt ccaaccggca aggtggatat ggcaattacg acctttatag
    12781 agtggataat gtcgatggca gttggtcaga acctgtcaat ctcggccctg aaatcaattc
    12841 cgcttccaat gaattatttc caaccttgct ttcccgggac atcctctcgt tcagctccaa
    12901 taggcccggc ggtaccggtg gcttcgacat ttatttggca aaaatattca ggaagacatc
    12961 tattgaagtc acacgcctcc ctgctcccat caatagtgaa agtgatgata tgagtctcat
    13021 attgaccgga gacaacagta aggcatattt ttcttctgca cgtcaggggg gaaagggcat
    13081 ggacgatatt tatcttcttc aaatcactcc cggaacagag cctgtcgtaa tccaacagac
    13141 actggtgata gtggatagtg taagggttca gcgtttgcag gatgcaagat gtgttttgaa
    13201 aacccttgca ggtgatgtcg tagcggatgg tgttacggat cgcaatggta gtctccaggt
    13261 gagtgtgacc aaggggcacc agtatatgct ggaggtagag tcacggaact atctgccata
    13321 tcaatcccct ttgtggggtg gtgagaataa aacagtgagg ctgcaacctt tgccatgcct
    13381 tactgtgaat ggatttgctc tggaacagga aaccaataag ttattaagag atgcggaaat
    13441 tgtatgggtc aatgattgtg gagaacctga gataaaggtt gtttccgaca gcatcggccg
    13501 atttgagctt tgtgttccac ctaattgttc aggagtcatc attggccgaa aggaagaatt
    13561 caacacggta cgggttcctg tcccggcttt aaacgagaat ttgaccatca atgtcaattt
    13621 ctccatggtt atgctttcta ttgtcaggga gccgatccga aagggagtga agctgattct
    13681 tgataatatt tattataact ttaatgaatc gaccatccgg ccttctacgg atcgtgaact
    13741 gaacgagctg gcaatattga tgaagcaata tccgaaaatg aggataaatt taatcgccca
    13801 taccgactct cgcggagatg ccaatgccaa tctgactctt tcaatcaaca gggccatgga
    13861 agccaagaaa tacctggtgg aaaaaggaat agctgaagac aggattgaga cggagggaag
    13921 aggggaaagc cagctgcgca atcggtgtaa ggatggagtc aattgtacag aggaagaaca
    13981 tcagtacaac agaaggatcg aagtcaaaat attggaaata tgagcagcta tccatagcca
    14041 ttgcttacct ttgcaccttc taaatccaaa ccatgtcgaa taaaaacaag cttcaaagat
    14101 ttgctgagat agctgtcctg cccaatgtat tggagaattt tgattttcac gacccgcacg
    14161 tggcggatac cttcggcaat agagttgata tcaaggggca ttggaaccgt ttgatgtttg
    14221 agcgtgacct gcccatagtg cttgaactag catgtgggaa gggtgaatac acgacccatc
    14281 ttgcagtagc agaccctgaa aagaattata ttggaattga tattaaagga gccaggattt
    14341 ggaaaggtgc taccatggca catgaccaag ggttgaagaa cgtggccttc ttgcgaacac
    14401 ggattgaatt gatccggtca ttttttgaaa agaatgaagt ttcagagata tggatcacct
    14461 ttcccgatcc attcctgagg gactcgaaat ccaatcggcg cctgacttct aatgccttcc
    14521 tggacagata tacaggaatt cttgtacccg gtgcaatcct tcatctcaag acagatgatc
    14581 ccaccctgta caaattctca ctcaaagtcc tggaaaaccg ggaagattat gagatcgtcg
    14641 aacatacgga tgacttctac aaggatgaga tgcctacgcc ggaaacgcat ttgctgacct
    14701 tctatgagca gcaacacctg gctcagggta agaaaattac ctatatcaaa tttagatacc
    14761 agggataatc ccggatcaat gtatcatttg caggtcaact tttgccattg ataaaagacc
    14821 tacttcaacc cgggagtaaa agggaaccgg agtggtgtca tgtgaagata aggaagtcag
    14881 gccgtaatgg cggaaaacaa aaaagccgga caactgatag ctgcccggcc atatctttat
    14941 catgtcagaa tcagatggaa gcgacgtgcc tggtaaggct gctctttagg tttgcggcct
    15001 tgttcttgtg gatgttgtta tgcttggcca atctgtcaac catgcttatt actttaggta
    15061 acatggttgt agcttctgac ttgtctgtca gttctctcag cttcttgatc atggtacgtg
    15121 ttgacttttt gtagtacctg ttctggatac gttttttagc atcctgtctg attctcttca
    15181 atgatgactt gtgatgtgcc atacaaaata ttaatttaaa agtatctttt ttaatccaat
    15241 ggagcgcaaa ggtaaaatta attttttgaa ttaggaaata aatttttaac agtatttcat
    15301 atatggaaaa tgttaatttt gcgtttcgct tgctataatt gaatttcaga cctcaaaaac
    15361 tacccctttc tgctttaaat gaggctttca agtgttaatg tttagctaaa tttagtaaat
    15421 atgcaccttg caggcaaaaa taaaagttta aaagtgtgca agaaaccaaa tataggccta
    15481 tctttgcctc attaaaaaat ttaataataa ctaaaacaat tcaatcatga acaaaggaga
    15541 tttgatcaac aagattgcag attctgcaaa gatcaccaaa actcaggctg gaaacgctct
    15601 tgatgctact atcaaagcta tctcttcttc tctgaagaaa ggtgacaaag ttactttgat
    15661 cggtttcggt actttctctg tttctaagag aagcgctaga actggtagaa accctcagac
    15721 tggcaaagaa atcaaaattg ctgctaagaa agttgcaaga ttcaagccag gtaagagcct
    15781 tgctgataca gttgcttccg gtaagtaatc agaagacact gaaagagatt aaagggcgat
    15841 ccgcaagggt tgcccttttt ttatcttcaa tagatcgccc aggtgacaga gttgtttgag
    15901 ggaatgaata agcaggtttc ttatttgatt tcgtagcaga tattcttgtc attgatcttg
    15961 tcattaggca tttaccgtac attgttgtcg caaatttcat acctttgtga tgaaatgtcc
    16021 ggatacatag gaaatatata taatcgtttg tctgaagacc gggcacacgg tcagaaacgg
    16081 ttcgctgtac tcatcgatcc ggataaacta cgcatgtcca acctggacca ggtacttaaa
    16141 gcctcggtgg atgcaggtgt agactatttc tttattgggg gtagcctgat agtcagcgat
    16201 gtcctggatc agttgctcat gtccattcgg caggcgacca atataccaac agttctattt
    16261 ccgggcaatt cctatcaatt gagttacaag gctgacggca tcctattcct gtccttgata
    16321 tcaggtcgca atcctgaatt attgattggc aatcatgtat tggcagcgcc ctaccttaag
    16381 ttcagtccgc ttgaaatcct tccgaccggc tacatcctga tcgatggagg tgttaataca
    16441 accgtaagtt atatctccaa cacccggccg atcccggcaa ataagccgga tatagcgagt
    16501 tgtacggcga tggccggcga gatgttgggc ctacgcctga tctttatgga tgccggaagc
    16561 ggtgcgctaa acccggtgcc tgcggaaatg atatcggctg tcaataatgt aatcagctcc
    16621 cctcttattg tcggaggcgg tatacgcacc ccggaggcgg caagggaggc ggcacgggcc
    16681 ggagcagacc tgatagtcgt gggtaatgcc atagaaaaag atccgggcct gattgaagat
    16741 atcgcttcgg ctattcatac atcaaggaca atttcctccc tgtgaagggc atagtcacca
    16801 aatctaccgg ttcctggtac actgttcggt ctgaacaggg agaacatctt gaatgccgca
    16861 tcgttggcaa gttcagactg aaggattttg aactgaccaa tcccgtggca gtaggtgatg
    16921 aggtggagta taaatgggat gaagtggaag gaaagggtgt cataaccagg attgggccgc
    16981 gaagaaacta catcgtccgc cagtcaccgc gcatgaagca caacctccac ctgattgcat
    17041 ccaatatcga ccaggccgta ttgattgtca ccattgtcca gccggatctc aagcccggct
    17101 ttgtcgacag atatctcctc atgacggagc cacacgatat accggccatc atagtcttta
    17161 acaagtccga cctatacgat gagggggcca cagctttgct cgactatttc cgcaatctgt
    17221 acgagaatat cggttatgaa accttcgccg tttctgcctt gaagcacgat ggcctggaag
    17281 ccctgaaatc gcgcctcatc ggccggacaa ccctcatcgg aggccagtca ggagtcggca
    17341 aaagcagcct ggtcaatgcc atgttctcag gatttgatct caggacaggt gagatatcgg
    17401 attacagtgg caaaggtgta catacaacta ctttcgcaga aatgtactca ctcgaagagg
    17461 gaggctctat catagatacg cccggtatca agaccctgtc tttcaacaat ctcacgccga
    17521 tggatgttgc tcacaacttt agggaattat tcaagatgtc agaacattgc cgttttggcg
    17581 ggtcttgcac gcataggaat gaacccggtt gtgcagtgcg ggaagccgta gacaagggca
    17641 ttatcaatga gatccggtat cagaattatc aggccatcat cgatgagctg aatgagcaaa
    17701 actattggga acgaaagaaa aagatgtaac cttgactgta tgctttgcgt acattttttt
    17761 ataacaaatc atgttatgga ttggaaagtt gaagataatc agttgagaaa agagttccgg
    17821 ttttctgatt tcaaggcagc aatggccttt atgcagttgg tggcatttga agctgaattc
    17881 ctcggacatc atccggagtg gaccaatgtg tataatacag tgcgtatcag actgtacaca
    17941 catgatgccg gaaatgttat cacagaccgt gatcatcgtt tggcagatgc tattgacagg
    18001 atttacaccg attattttgc ttaagattac gagttttttt aaagtcgatg gtcaggggcc
    18061 tgcctttggt ctatttgttc caaatcgtat gtatttatgg accgatcgat tttttttagt
    18121 caatggggtg gatttgatat ggcggagccg ataatgaaaa gctcggtttt ccggggcgta
    18181 ataccggatg ttccttcggt attatcacgg tccatgaaaa taagccactt tctttttctc
    18241 gatgtcaata aacaaaggat gttttcggag agtatttcca tgggcgttat ctataccttt
    18301 ttctgtttca gagtccgggg atgaatcctt tttgtaattg gtgtaatttt gcaactttag
    18361 aaaggcggga ttgttacctt ccatgatgct ttctgtgtga atactgaaag gtttgccggc
    18421 atttcttagc cgcgactaca tttatattga taatgaaaag attcatgacc agaccttttt
    18481 ttatatccct gatccttaca ttatcaggaa ttatttttct tttttcttca accaaaaacg
    18541 ataccggcgt gcaccaaagc atatacgatt ttaatgccac aaccttagat ggcgctcctt
    18601 tccattttgc tgatctgaaa ggcaaaacca tcctggtcgt caacacagca agtaattgtg
    18661 gatttacacc gcaatttgaa agtcttgaag ccttgtaccg gaaatatgcc gaccgcggtt
    18721 tagttattgt cggatttccg tgtaatcagt tcggaggcca ggaacccggc gggaaagaag
    18781 aaacagagaa gatatgcttt gccaaatatg atgtaacttt cccaatgatg gagaagacag
    18841 atgtcaatgg tcgaaatgct catccactat tcgttttcct gaaggaccgg cttcccggct
    18901 ttcccggaaa gaaaatcaag tggaacttta ccaagtttct gatcaacgct gaaggcaagc
    18961 cgattaaaag gtttgctccc tgggataagc ctgagaaaat tgaccattat ttagagaatc
    19021 atatctggaa gaaatagatc aattgtcagg atctttgtca ggtttcaatt tcgctttttg
    19081 caggtcagta tccactatca aaaactgttg gtgacttgaa tcctgctcat aataaaatcc
    19141 tcaatgcatc aaaaagctta tcgagatcag cttgttcgtt gaatccattg attgaatacc
    19201 ggatatagaa ctgatcaccc tgggccatca cgggaatttg gatattaaat gtacgataca
    19261 acctgtcatg tagtgcctgg ctatccgttg tatctatttg cgcggcatac atctgtgctg
    19321 taaaatcatc attgattggt gcgattggca gatggcctgt gatggttgtc agggttgatg
    19381 cattcttttg cactaattta cgacattcgg aagcgactga ccgccaattg t
//
LOCUS       DAOQMC010000012        18822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1023_length_18822_cov_4.454734, whole genome shotgun sequence.
ACCESSION   DAOQMC010000012 DAOQMC010000000
VERSION     DAOQMC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18822
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1023_length_18822_cov_4.454734"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2745)
                     /locus_tag="PKV31_01060"
     CDS             complement(<1..2745)
                     /locus_tag="PKV31_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91466.1"
                     /translation="MKKNVQYNFLEALVFFLLFISFKQGITQTTIFRERFNIAPNTEA
                     NGATSGLSEEGIAWTATCPLCFINNPPSPAGRHFDVQNGEFENKANEGVGQWITTGAI
                     DISTATKLDFTVDYHSNLPWPSGGNMESNNECGGCAGDPDNYPNTGPCGSCWDFINVQ
                     LILDGTTVYNETVGNGNNVQTYQFSFSSACFPPGTYSNAVIRIRTQTWSLGDPEIIWF
                     DDVHLVAYQAPTITLTPAGPYCVDDNNPKALTASPSGGAWSGPGIIGNTFTPSIAGAG
                     THILTYTYQSAATGNCPVTETMQVVVNPAPTATAGVSPTNICEGDDFQLNSSGGTSYQ
                     WSGPNGFTSNQQNPTITNSGSTNAGTYTVTVTDGNNCTATATVDVTINALPTATANAN
                     PNPICEGQTLNLTSSGGTSYSWTGPGGFTSSDQNPVINNISTTQAGTYSVIVTSNAGC
                     NATTSISVTVNTNPTVSINPIPDLCTSDPIYTPAGNPTGGTWTFNGIPISDIDPSAYT
                     PGSYILVYNYTDGNGCSNSASITVNIVDCGCINPPDVDAGADASICSGNTYTLSGTAT
                     NTSVYSWSTGGDGTFNNPNILNPTYTPGPGDIASGIVIITITTPDPDGLGPCIGDTDA
                     MALTINQTPVLNFPGGVSICENTCQTITMSVTGGSGSYTANLVITSPINYSIPAITIN
                     GTANFTICYQGVLPSFNSATNTLQVPPTIVPTGTNVTFALTQLVDNNTSCQGVLSGSS
                     NTSITVFTNPSVTANPIGPLCSDGPPVNLSGNPTGGTWSGTGVINGTFNPTVSGPGFF
                     QLTYTYTDGNGCTGTATTTVQVENCNCNLGIVVNAGPDISSCISDPILLQGNVINSTN
                     YYWTTSGDGVFSNINDLNATYTPGIGDVANGSVTLILIVPDPDDDGPCTDIPDQLTII
                     F"
     gene            3177..3794
                     /locus_tag="PKV31_01065"
     CDS             3177..3794
                     /locus_tag="PKV31_01065"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB91467.1"
                     /translation="MSVTNAFVIVSGLLSVLAFRNPALLYKLIGWPHRSSTEREYYRL
                     FTGGLVHGDYIHLLVNLFVMYQFGAIVEMEYKVLFGENGVYYFLALIVLGVIVPNLFD
                     LMLHKNQPNYRSLGASGSVSAVLFAYVLFNPWSKLYLYGILPLYSIVAAVLYIVYSVY
                     QDKKGKDNINHMAHLIGAVFGFVFTLALKPSLGPFFVQQLLSFSF"
     gene            complement(3784..6036)
                     /locus_tag="PKV31_01070"
     CDS             complement(3784..6036)
                     /locus_tag="PKV31_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNB91468.1"
                     /translation="MTYKLSEYIEQKMGENHPERVNELHLRIFIPIQQEETKTKLFNI
                     LNCDTDRKNVAFNNLSPNEHKVLSDFSFQISEDERIDERTTWTINQRIEPETEIYGMG
                     DKAVVPNLRGRKCIIWGTDTYAFEYGAEPLYKNIPFYIRNDRNGVLGVFINNTHRIEF
                     DFGYSDERIHISGSGGHIDMYLIFGESPLEVVTNYTKLTGTPHMPPLWALGFHQSKWS
                     YVPEQALMDVAAKFRELNIPCDSIYLDIDYMRGYRVFTWDKRRFPDPKAMLKKLRHQG
                     FQIVAILDPGIKIDKRYAIYKEAISEDFFIKNPDGSITSGPVWPGYCHFPDFTSRKVR
                     RWWADHVAELVRVGLAGIWNDMNEPALFNKADMIQTQRTLNEDVRMNFEGESGDFAEG
                     HNLYGMLMSEATMEGFRQAGDRRPFVLTRSTYAGGQKFAAVWTGDNVATWEHLRIANY
                     QCQNLALSGFSFAGSDIGGFVGNPDGELFCRWLQLALFHPFFRNHSSKDFAEQEPWSF
                     GEKWTDCARQIIHWRYRLLGYIYTVFRRYVHEGIPMIKPVNFVHAKEKRPPCFDQFYL
                     GDALLAIPVMEPGQSGVRISLPEGYYYDVNSARQFIGNNDYFIRVKMDAAPALLKGGH
                     ILPIWPAMQYVNEKPLEEMEIWCGFSDAEKVISRMYLDEFDGHSYLEGNYQETVFEYN
                     NCQTYVLININQSGYYQAAIRKYTFKFPGFKNKIKSIESDGNKLEISKDDPIIVLEKM
                     PEKIRIDLKS"
     gene            6348..8003
                     /locus_tag="PKV31_01075"
     CDS             6348..8003
                     /locus_tag="PKV31_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB91469.1"
                     /translation="MHEKIWLKHYPSNIPTEVRYDEYPNLNSFIRATIREYGDQVAFI
                     SMDKKLTYNQLDKYADQFAAYLASLGMKPGDKLALMMPNILQYPIALFAAIRAGYIVV
                     NTNPLYTPREMKLQFQDAGVTAIVIAENFAYNLQQIIGETKIKHVITASIGEMLGGLK
                     GPLVNFVIRKVKKMVPAYNLPGSVSFKKALGEGISKPVPHHDSGSEDTVIFQYTGGTT
                     GIAKGAMLTNENLLANLEQVKAIFLSQLRKNEEVGFCALPLYHIFAFTVNCLVLFSLG
                     CPNVLIVNPKDIPSLIKEWKKNSPSIFPAVNTLFNALANNDQFRQLDFSNLKIPIAGG
                     MALQSTVAENWKKVTGVRIVEGYGLTETSPVASVNPLDGREKIGTIGWPVPSTDMRIV
                     NDEGVEVAQGETGEIQIKGPQVMKGYYNKPEETNKVLKDGWFSTGDVGLMEADGYFRI
                     VDRKKDMILVSGFNVYPNEIEEVAVSFPKVLEAAAVGVPDEKSGEHVKLFVVKKDSSL
                     TAEELLEYMKKNLTQYKHPKEIEFRDDLPKTNVGKILRRELRN"
     gene            8133..11897
                     /locus_tag="PKV31_01080"
     CDS             8133..11897
                     /locus_tag="PKV31_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB91470.1"
                     /translation="MSREIQIRKTIADIHQFYVVKEKIDRKVAKSLGIHVEGDASFFK
                     VNKDAVASLIDEQPELLTLEIPYGQDGRSVQVLLYNKKPYTNSAIVTTNTGEKYPMNK
                     AAFYRGIVAGQENSLAAISFFDGEMSGVISCQEGQFDIGKVDKKDIHVIFQSNTLSYK
                     YNFNCGVIDDDSVDDAEPSLDVRNANDCVNIFFETSYSIYQNKGSVSAVQNYVTGFFN
                     VVTTLYDNESVNVAISEIYVWTSQDPFPTNSSNSALNYYTDYRTDFNGDIAQLLAKTS
                     NGNGGIAWLDVLCNTSTKHSYSDISMNYSGYPTYSWTVMVVTHELGHNIGSRHTHWCG
                     WQGGAIDNCYQVEGNCSPGPPPTNGGTIMSYCHLTNYGINFSNGFGQQPGNKIRQEVQ
                     QASCLSSCSNQCPTFVLNGTVTNVSCNGGGNGSISVVEPTEGTSPYTYTWSNGATTKD
                     ISNLIPGDYTLSITDALGCPGQATFTVTEPTAIEIQGQSYDASCYGSSNGSIIITVNG
                     GTPGYSYNWSNGNTAPSLVNIPAGNYFLTVTDSKFCTKTETYYVGQPSQLLISSTISN
                     ISCFGANDGIISVGAYGGTPLYSYQWNNNSNESQLIQLGPGTYTVTVTDDHSCQVSGS
                     YTITEPSQLTGQVVTTDASSNNAADGTAEANMNGATPPYSYLWSNGQTSKKIVNLPVG
                     NYSVTVSDANGCQLVLYCSIGAPDCTLQLQSDKTDISCFGANDGTALANASNPNGSVN
                     YLWSNGATSQTITNLAPGTYSVTVSDNICQKVSSVEVVSPGPINIIPTLINPACDDNN
                     GSISINVNGGTAGYTYLWSNGATTQNISALNAGNYTVTVLDAHNCSSQQSFTLTAQDN
                     QAPTLLNDKITLYLDTNGNAELTTIPTSYLFEDNCGTVSVVFQNTSFDCDKLGKNNIT
                     ATGTDEAGNSIQVNLEITVIDSIKPFIQCPSDFTIGICQGAPVWDDIIYSDNCQVVTI
                     QQTAGYAPGTIFPIGVTEVAYQVTDLSGNQNSCSFSIHVAEGLALNVSKTDMACNNVA
                     NGSATLDTKGFVEPYTILWSTGANTPSITNLGPGDYSVTVTDGNGCVATQTVTIINPA
                     PLEMEVGSVTQASSETAADGAISISVSGGTPAYSFSWTKNGEFISSDQNPSGLTHGVY
                     KVEIKDKNGCVIIGAQITVGFKLGNSNWDKGTVLVYPNPADQRLVVASASMEDSMLEI
                     YDILGRKIDLDFSKGFEKIFVNTESLSNGSYILRLESGNQWYVTKFVIIH"
     gene            12000..12368
                     /locus_tag="PKV31_01085"
     CDS             12000..12368
                     /locus_tag="PKV31_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNB91471.1"
                     /translation="MNIYIGDSKEKGRGVFTSLPIKAGDVIEICPVVVIPKDEIELID
                     RTVLYDYYFLWEEGCAAMALGYGSLYNHSYSPNVEYIMDFEDSVIIIKSLRDIASNEE
                     LTFNYNGDPFDKERIWFDPM"
     gene            12481..14466
                     /gene="rpsA"
                     /locus_tag="PKV31_01090"
     CDS             12481..14466
                     /gene="rpsA"
                     /locus_tag="PKV31_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNB91472.1"
                     /translation="MSDNENLLQESPESTPAAPKKTAHDAFDWTISKKNHIAYSAEET
                     QKYLEDYEKTLTLIKDNEIVKGLVKSISSGDVVLDINYKSDGIVSLSEFRDTPDLKVG
                     DYVDVYVESQEDAKGQLVLSRKKAKLLIAWDTIVDSFNNGTIIKGTVISKTKGGLIVD
                     AYGLETFLPGSQIDIKPIIDYDAYVGKTMEFKVVKINETIKNAVVSHKALIESDIAEQ
                     REHIIAGLEKGQVLEGVVKNITDFGAFMDLGGVDGLLYITDISWGRINHPEEVLQMND
                     KINVVVLDFDDNKKRISLGLKQLTPHPWDVLAMDISEGSIVKGKIVNVEDYGAFLEIS
                     PGVEGLIHVSEVSWSNQPVVARDFFKLDQEFEAKVVTIDRDERKMSLSLKQLQEDPWT
                     EIENKYPVNSRHTGTVKNLTPYGVFVEIDNGIGGMVHISDLSWTKRFSHPSEFTKVGD
                     QIEVVILEIDKANRKLSLGHKQIEENPWDAFEAVFPVGSYHEATVIRRDDKGATVQLP
                     YGLEAFAPIKHLKKEDGSTAEVEESLTVKVIEFNRDDKRILVSHSRYLEDIKKEADNL
                     IKRDKESADNETKSAIKTQQAKLEKTTLGDIDAFSQLKQQFDEAGEKKAEEAKEELAL
                     KAKKSTKATKAKEVVAEETAATEGGDTPEDSEKGEDA"
     gene            14662..17103
                     /locus_tag="PKV31_01095"
     CDS             14662..17103
                     /locus_tag="PKV31_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNB91473.1"
                     /translation="MADNNFLKKLKSILASIRIRSNKIDIFLEKRPGLNFVLKAGISA
                     LLTGFICLFVLIFSARIGVFGGMPTDDELKEISNPEGTELFADDGVTLLGRYYIENRS
                     NITYDSLPENLIHALIATEDIRYYEHNGIDWKSWARVLFRTILMQESASGGGSTISQQ
                     LAKNLFKRRTYWRGTTLINKMREIIIAQRLESMYTKEQLLTLYFNTVSFGGNVYGIDI
                     ASKQYFKTSPRFLKTEEAATLVGMLKATTKYNPLNNIDLAKKRRNTVLNKMEKYQYIT
                     QKQLDSLSALDIKLNYVKETHTEGIATYFREFARLDLDKQLAHLKKNDGSSYNLYTDG
                     LKVYTSINIPMQNYAESAVADQMKELQELYYQQMRWIKLPWKDTAFLHKAIMNTDRYK
                     SMVTAGLSQHYIDSVFNVPYEMEVYSPQGPKTMKMSPKDSLIHYLQFLTAGFMAADPS
                     TGFIKAWVGGIDYKYFQYDHVKAKRHVGSTMKPIVYAAALEKGLDPCAYYDNRQKTYP
                     QFENWSPKNFEGGYGGEYSMAGALAKSLNCATVDIMLKTGIDTVVDFAKRIGVTSTIP
                     YEPSVSLGAVDISLYDMITAYSTIIDRGQKPALTYILKVVDSEGNVLIDNTTPEAQEK
                     VQVMNPENADIMRSMMQGVINHGTGGSIKWRYSITEDLAGKTGTSQDCSDGWFIASNP
                     KLIAGAWVGGEFPEIHLTGNFTGAFTALPIVGMFLRSVEKDKSLNDYVKEKFPELSQE
                     AKNRLNCPYSHYVADSSAIDSTGLDSLGNPIITDDDFHEQDDESPKVDTTKKKKFFEG
                     LFNRKEKKKVDNENN"
     gene            17132..>18822
                     /locus_tag="PKV31_01100"
     CDS             17132..>18822
                     /locus_tag="PKV31_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB91474.1"
                     /translation="MEIVETSGILIETLPGADIPPVESTLIELYKKLVYPRLIEEKML
                     NLLRQGKISKWFSGIGQEAISVGVTAALDEDEYIFPMHRNLGVFTSRNMPLETLFSQW
                     QGTEGGYSKGRERSFHFGSVPHHIVGMISHLGPQLSYASGVALGHKLAGNRKVTAVFS
                     GEGGTSEGEFHEALNVAAVWSLPVIFVIENNGYGLSTPVNEQYRCERISDKGKGYGMR
                     AITIDGNDIVNVFNTVRILSEEIKADPHPVLLECVTFRMRGHEEASGIKYVPKELLEY
                     WSTQDPILRYEKYLLERNLMNENDFLSIRSKFKEEINEAVNNALSHSPIVPSTQTELD
                     DVFAPFEYNEISGGPVETELRMIDAISDGLNEAMKKHSNLILMGQDIAEYGGVFKITD
                     GFLERYGKDRVRNTPLCESAIIGTALGLSLKGYKAMVEMQFADFVTCGFNQIVNNLAK
                     LHYRWGQHADVVIRMPTGGGVGAGPFHSQSNEAWFTHVPGLKVAYPSNPYDAKGLLLT
                     AFEDTNPVLFFEHKALYRSQIRMVPEGYYNLPFGKAAIARKGEGMTIITYGMGVH"
BASE COUNT         5530 a         3526 c         3998 g         5768 t
ORIGIN      
        1 aaagataatt gtcagctgat caggaatatc cgtacagggg ccatcgtcat ccggatctgg
       61 cacaattagt atcaatgtta cagaaccatt ggctacatcc ccgattccgg gggtataggt
      121 tgcattcaag tcattgatat tggaaaatac accatcaccg gatgttgtcc agtaataatt
      181 tgtactgttt attacatttc cctgcagtag gattggatct gatatgcagg atgatatgtc
      241 cggtcctgca tttactacga tacctaagtt gcaattacag ttttcaacct gtacagttgt
      301 agtggcagtt ccggtacatc cgttgccatc agtatatgta taggttagct gaaaaaatcc
      361 ggggccactc accgttggat taaatgttcc attgattact cctgttcctg accaggtgcc
      421 acctgtaggg ttccctgaca aatttaccgg aggcccatct gaacataaag gtcctattgg
      481 attagctgtg acagaaggat tggtaaatac tgtaatggat gtattactgc ttcctgacaa
      541 aactccctgg caactggtat tgttatccac gagttgagtt agggcaaagg ttacattagt
      601 tccagtcggg actatagtcg gaggtacctg caaggtgttg gtcgcactgt taaatgatgg
      661 caaaactcct tgatagcata tagtgaagtt ggccgttccg tttatggtta ttgccgggat
      721 agaataattg atgggtgatg tgatgaccag atttgccgtg taggaaccac ttcctccggt
      781 tacgctcatt gtaatggttt gacatgtgtt ttcacaaata ctgacacctc ccggaaaatt
      841 gagtaccgga gtttggttaa tggtgagcgc catagcgtcc gtgtcaccta tacacggtcc
      901 taatccatcc ggatcagggg ttgtgatggt aataataaca atgccggaag caatatcacc
      961 cggcccgggt gtataggttg gattaaggat attcggattg ttaaatgtgc cgtctccgcc
     1021 ggtactccag ctatagacgg aagtattggt cgcagttcca cttaaagtat aagtattgcc
     1081 actgcagatg gaagcatctg cgcctgcatc tacatcaggg ggattgatac aaccgcaatc
     1141 aacaatattt accgttatac ttgccgaatt gctgcatcca ttaccatctg tgtaattgta
     1201 gacaagaata tagctacctg gtgtgtatgc tgagggatca atatcactaa tcggaatgcc
     1261 attgaaggtc catgttccac cggttgggtt cccggcaggt gtatagatgg gatcagaagt
     1321 acaaagatca gggattggat taatcgacac tgtcggattg gtattgactg tgactgatat
     1381 ggaagtagta gcattacagc ctgcattgct cgtgacaatt acactgtagg tacccgcctg
     1441 agttgtcgaa atattattga ttaccggatt ttgatcactg gaagtaaatc cacccggtcc
     1501 tgtccaggaa taggatgtgc caccggatga tgtcaaattt aatgtttggc cttcacaaat
     1561 tggattggga ttggcattag cagtggccgt tggaagggca ttgatggtta catcgacagt
     1621 ggcagttgct gtacaattat taccatcggt tactgtaact gtatatgtcc cggcatttgt
     1681 tgaaccggaa ttggttatgg tcggattctg ttgattggaa gtaaatccat ttggaccgct
     1741 ccattgatat gaggttccac cggaagaatt caattgaaaa tcatcaccct cacaaatatt
     1801 ggtaggagat actcctgctg ttgccgtggg agcaggattg accaccacct gcatggtttc
     1861 cgtaacaggg cagttgccgg tagcggcaga ttgataggta taggttaaaa tgtgagttcc
     1921 tgcccctgcg attgatggtg tgaatgtgtt cccgataatt cccggtccac tccatgcacc
     1981 cccggaaggg gatgctgtca gtgcttttgg attattgtca tccacacaat aggggcctgc
     2041 cggcgtcaaa gtaatggtcg gtgcttggta agcgacaaga tgcacatcat cgaaccagat
     2101 gatctcaggg tctcctaatg accaggtttg ggttcttatt cttattactg cattggaata
     2161 tgttcccggt ggaaagcaag ctgaagaaaa actaaattgg taagtctgaa cattattccc
     2221 attgccgacg gtttcattat atacagtagt tccgtccaag atcaactgga cattgataaa
     2281 atcccaacaa ctaccacatg gtcctgtatt gggataatta tccggatctc cggcacaacc
     2341 tccacattca ttattagatt ccatattacc gccggaaggc cagggtagat tactatggta
     2401 atcaacggta aaatcaagct tcgtagctgt cgaaatatca atggcacccg ttgtaatcca
     2461 ttgtcccacc ccttcattgg ccttgttttc aaattcgcca ttttggacat caaaatgcct
     2521 tcctgccgga gatggtgggt tattgatgaa gcaaagtgga caggttgctg tccatgcaat
     2581 tccttcttca ctcaatcctg atgtagctcc atttgcttcc gtgttgggtg caatattgaa
     2641 cctctccctg aatatcgtag tttgagtaat tccttgctta aatgagatga ataataaaaa
     2701 aaagactaat gcctctaaga aattgtattg tacattcttt ttcaaaatat tccgacctta
     2761 aattttccgg ttgattaact atatactttg acaatccttg acaaggaaat tgtgcacaaa
     2821 gttaatcttt tatttatttc cctttaagaa tcatctatat tttttagtac tgttttcatt
     2881 ggctatgggt tcttcatgtt gtacattact ttttgaaatt caagtaattg gacatgcttc
     2941 aaatctatat gaatattaag gttttataga aattatatga atacgttgat ggaataaata
     3001 ttcatttttt atgtaaggaa taaggttcct tgtacttttt atcaaagctt tttatccatg
     3061 cgggaaatgg atataaatta cttttattag ttcatttagt cgatgaaatt ttcccatgtc
     3121 tgaccagatc atagtgttga aggattatct tcgcattcaa aataacttca acttttatgt
     3181 cagttaccaa tgcatttgtg attgtttccg gcctactttc ggttttggca ttcaggaatc
     3241 cggctctttt gtacaaatta attggatggc cacatcgttc ctcaactgaa cgggaatact
     3301 acaggttgtt caccggagga ttggtccacg gagattatat acatcttctg gtcaaccttt
     3361 ttgtgatgta ccaatttggt gcgattgttg aaatggagta taaggtactg ttcggagaaa
     3421 atggtgtgta ttattttctg gctcttattg tactgggtgt cattgttcca aatttattcg
     3481 atctaatgct acacaaaaac caacccaatt acagaagcct tggagcatcg ggttcagtta
     3541 gtgcagttct gtttgcttat gttcttttca atccatggag taagctatac ctttatggca
     3601 tcttgccttt gtactctatt gtggcagctg tcctatacat tgtatatagt gtataccagg
     3661 acaaaaaagg taaggataac atcaaccata tggcccacct aatcggagcc gtttttggtt
     3721 ttgttttcac cttggctttg aaaccttctc tcggtccttt ttttgttcaa cagttgcttt
     3781 catttagctt ttgaggtcga tccttatctt ttcaggcatt ttctccaaaa caattatcgg
     3841 gtcatcttta cttatttcca acttgttgcc atcgctttcg atggatttaa ttttattttt
     3901 gaatcccgga aatttaaatg tatatttcct gatagcggct tggtaataac ctgattgatt
     3961 gatatttatt aagacatatg tttggcaatt attatattca aacacagttt cctgataatt
     4021 gccttccaga taggaatgac cgtcaaactc atcaagatac atacggctaa tgactttttc
     4081 cgcatctgag aatccgcacc atatttccat ttcttccagt ggtttttcgt ttacatactg
     4141 cattgcaggc cagatgggta atatgtgtcc gcctttgagt agggccggtg ccgcatccat
     4201 ttttacccga ataaagtaat cattatttcc tataaattgc ctggccgaat tgacgtcata
     4261 atagtatcct tcaggtaggg atatcctcac tccgctttga ccgggttcca ttaccggtat
     4321 ggccagtaat gcatcgccca gatagaattg atcaaaacag ggaggtcttt tttctttggc
     4381 atgaacaaaa ttcaccggtt tgatcattgg aatgccctca tgcacatatc tccgaaaaac
     4441 cgtatatatg tatcccaata atctataccg ccaatggatg atttgacggg cacagtctgt
     4501 ccatttttct ccgaaagacc aaggctcctg ttccgcaaag tcttttgacg aatggtttct
     4561 gaaaaatgga tggaaaagtg ctagttgtag ccaccgacaa aacaattctc catcaggatt
     4621 gcccacaaag ccaccaatat cgctcccggc gaacgaaaat ccacttaatg caagattctg
     4681 acattgataa tttgcgattc ttagatgctc ccaggtggct acattatcac ccgtccacac
     4741 tgccgcaaat ttttgccctc cggcataagt tgatctggtt aatacgaaag gcctcctatc
     4801 tccggcttgc cgaaaacctt ccattgtagc ttccgacatc aacatgccat atagattatg
     4861 tccctcggca aaatcaccgc tttccccttc gaagttcatc cttacatctt cattcaaagt
     4921 gcgttgtgtt tgaatcatat cagccttgtt gaacaaagca ggttcgttca tgtcattcca
     4981 tatacccgcc agaccaaccc ggaccaattc agctacatga tctgcccacc atcttcttac
     5041 tttgcgggac gtaaaatccg ggaagtgaca atatcccggc caaacaggac cagatgtgat
     5101 cgaaccatcc gggtttttta tgaaaaaatc ctcactgatg gcctccttat agatagcata
     5161 ccgtttatca atttttatac ccggatcaag aatggcaaca atctgaaagc cctggtgtct
     5221 caacttcttc aacattgcct tcggatcagg aaatctcctt ttatcccagg taaatactct
     5281 gtatcctctc atatagtcga tatccagata aatgctgtcg cagggaatat ttagctctct
     5341 gaactttgct gctacgtcca tcaaggcttg ttccggtaca taggaccatt tactttgatg
     5401 aaaacccaaa gcccataatg gcggcatatg tggcgtgcct gtcagctttg tgtaattagt
     5461 cactacttcc aatggcgatt ccccgaaaat caagtacata tcgatatgcc ctccacttcc
     5521 tgaaatatgt atccgctcat ctgaataacc aaaatcaaat tcgattcgat gtgtattatt
     5581 gatgaatact cctaaaactc catttcgatc attcctgata taaaaaggaa tatttttata
     5641 aagtggttct gctccatatt caaatgcata ggtatccgta ccccaaatta tgcattttcg
     5701 gcctcttaga ttggggacaa ctgccttgtc tcccataccg tatatttctg tttcaggctc
     5761 tatccgttga ttgattgtcc atgtagttct ttcatcaata cgttcgtctt ctgaaatttg
     5821 aaaggaaaaa tcagataaga ctttgtgttc attcggagat aaattattga aagcaacatt
     5881 ttttctgtct gtatcgcaat tgagaatatt gaaaagtttt gttttcgttt cttcctgttg
     5941 tattgggata aaaattctca aatgaagttc attaaccctt tccggatgat tttcacccat
     6001 tttttgttcg atgtattctg aaagcttata agtcatatgt ttggtatgta aaagaccaag
     6061 ataaagaatt ttctctacag attgtattat ttgaacttta ccatatacga atggtgaagt
     6121 caaccaatat ttaagcaatc ttaataggta ggatggagat gcaatcaata attcctgcgt
     6181 aatgttcagg aaaattaggt aaaatggcga ctcggctaaa aaatcttttt tattttcgga
     6241 ttatcataaa ataaattttg gttatatata ggtattcagc taattttgcc acttatcata
     6301 ataattgttg cgataatatt tatcatttga aacattaaaa tacagatatg cacgaaaaaa
     6361 tatggctaaa gcattatccg tctaatatac cgacagaggt aagatatgac gagtatccca
     6421 atttaaattc atttattcgc gctacaatta gggaatatgg tgatcaggtg gcgtttatca
     6481 gtatggataa aaaactgact tataatcaat tagataaata tgccgatcag tttgctgcct
     6541 atttggcctc cctgggtatg aagcctggcg ataaattggc cttgatgatg cctaatattt
     6601 tacaatatcc tatagcatta tttgccgcaa ttagggccgg atatatcgta gtgaatacca
     6661 atcctcttta tacgccgaga gagatgaaat tacaatttca agatgccggc gtaactgcaa
     6721 tagtcattgc agaaaatttt gcatacaatc ttcagcaaat tatcggagaa actaaaataa
     6781 aacatgttat tacagcaagt atcggagaaa tgcttggtgg cctgaaaggc ccgttggtta
     6841 actttgtcat acgtaaagtc aagaaaatgg ttccagctta taacttacct ggttctgttt
     6901 ccttcaagaa ggctttaggc gagggtattt caaaacctgt acctcaccac gattcaggat
     6961 ctgaggatac tgtaatcttc caatataccg gtggtactac tgggatagca aagggcgcca
     7021 tgttgaccaa tgaaaatctt ttggccaacc tagaacaagt aaaggccatc tttttgtccc
     7081 agcttaggaa aaatgaagaa gttggattct gtgctttgcc tttgtatcac atttttgcat
     7141 ttactgttaa ttgtttggta ttgttttcat tgggatgccc taatgttttg attgtaaatc
     7201 caaaggatat tccttcattg ataaaggaat ggaaaaagaa cagtccatct atttttcctg
     7261 ctgtcaatac ccttttcaat gccctggcta ataatgatca atttcgacaa cttgatttca
     7321 gcaatctgaa gattccaatc gccggtggta tggccttaca gtcgacagtt gccgaaaatt
     7381 ggaaaaaggt tacaggtgtg cgcattgttg aagggtatgg attgacagaa acttcaccgg
     7441 ttgccagtgt aaatccactg gatggacggg aaaagatcgg gaccattgga tggcctgttc
     7501 cttcaacgga tatgagaatc gtgaatgatg aaggcgttga ggttgcgcag ggagaaaccg
     7561 gtgagatcca aattaaaggt cctcaggtta tgaaaggata ttacaacaaa ccggaagaaa
     7621 caaataaggt tttgaaggat ggttggttta gtaccggaga tgtgggactg atggaagccg
     7681 atggttattt caggatagtg gaccgcaaaa aggatatgat tctcgtttca ggatttaatg
     7741 tatatcccaa tgaaattgag gaggtggctg tctccttccc aaaggtgctg gaagctgctg
     7801 cagtgggagt acctgatgaa aagagtggag aacatgttaa attatttgtc gtaaagaaag
     7861 attcgtcttt gacagccgaa gaattgctgg aatacatgaa aaagaatctt acccaatata
     7921 agcatccaaa ggaaattgaa ttccgcgacg acctgcccaa gacaaatgtt ggaaaaattt
     7981 tgcgtcggga attgagaaat taaataattt tgcatgtcga atcatctgtg tgtattgata
     8041 ttataaatgt ttattttttt taaatgaaac ttagctgttt actacctcta tttgctctat
     8101 ttctagttac gggattatct gctcaaaatc aattgtcacg tgaaatacag attcggaaga
     8161 caattgctga tattcatcag ttttatgttg taaaggaaaa gattgatcgg aaagttgcta
     8221 aatctttggg gattcatgtt gagggcgatg cgtcattctt caaggtaaac aaggatgctg
     8281 tggcctcact tattgatgaa caacctgaat tgcttaccct tgaaattcca tatggacagg
     8341 atggcagatc ggttcaggta ctattgtaca ataagaaacc ttataccaac tctgctattg
     8401 tcacaaccaa tacgggagaa aagtatccga tgaataaagc tgctttttat cggggtattg
     8461 tcgcaggtca ggaaaatagt ctggcagcaa tcagtttttt cgatggagag atgtctggcg
     8521 ttatttcttg ccaggaaggt caattcgata ttggaaaggt tgacaaaaaa gatattcatg
     8581 tgatctttca atcaaatact ttgtcctaca agtacaattt caactgtggt gttatagatg
     8641 atgattcagt agatgatgct gagccttctt tggacgttag aaatgccaat gattgtgtga
     8701 atattttctt cgaaacatct tattctatat accaaaacaa gggaagtgta tctgcagttc
     8761 agaactatgt tacaggcttt tttaatgttg taacaacctt atatgacaat gaaagtgtga
     8821 atgtagccat ttctgaaatt tatgtttgga catctcagga tcccttccca actaacagtt
     8881 cgaatagtgc gctaaattac tataccgatt atcgaactga ttttaatgga gatattgcgc
     8941 aattgcttgc caagacatct aacggcaatg gagggattgc ctggttggat gttttatgca
     9001 atacttcgac taaacacagt tattccgata tcagcatgaa ttacagtggt tatccaactt
     9061 actcatggac agttatggtg gttacccatg agttgggtca caatatcggt tcgcggcata
     9121 cccattggtg tggctggcaa ggcggagcta tagataattg ttatcaggtc gaaggcaatt
     9181 gttcacccgg acctccacca accaatggtg gaactatcat gagctactgc catttgacca
     9241 attatgggat aaatttttca aatggttttg ggcagcaacc cggtaacaaa atacgtcagg
     9301 aggttcagca ggcatcctgt ttgtcaagtt gttccaatca atgcccgact tttgtattga
     9361 acggcactgt tactaacgtt tcttgtaatg gtggaggtaa tggctcaata tccgtagtgg
     9421 aaccaactga gggtacttct ccttatacat atacctggtc aaacggtgct acaaccaaag
     9481 atatttccaa tctgattcca ggggattata cattgtcaat aactgatgca cttgggtgtc
     9541 ccgggcaagc gacttttaca gtaacggagc caactgcaat tgagattcag gggcaatctt
     9601 atgatgcatc ttgctatgga agttccaatg gctcaataat aataactgta aatggtggta
     9661 ctcccggcta ctcttacaat tggagtaatg gaaataccgc tccatcctta gtaaatattc
     9721 ctgcaggtaa ttattttctg actgttacag attctaagtt ttgcaccaag actgaaactt
     9781 attatgtagg tcaaccgtca cagttgctaa taagttccac aatttcaaat atatcctgtt
     9841 tcggagctaa tgacgggatt atttctgtgg gtgcctatgg aggcacgcct ttatattcct
     9901 atcaatggaa taataattcc aatgagtcac aacttattca acttggtcct ggtacatata
     9961 cggtgacggt aacagatgat cattcttgcc aggtttcggg ttcctatacc ataactgaac
    10021 catctcaact aaccggacag gtggttacta ctgatgcatc gtcaaataat gctgctgatg
    10081 gtactgctga ggccaatatg aatggagcaa cgcctcctta ttcctattta tggtccaacg
    10141 gtcaaacctc aaagaaaatc gtgaacttgc cggttggtaa ctattccgtt actgtttcag
    10201 atgctaatgg atgccaattg gtactttatt gcagcattgg tgcacctgat tgtacacttc
    10261 agcttcaatc tgacaaaaca gacatttcct gtttcggagc taatgatggg actgctttag
    10321 cgaatgcatc aaaccctaac ggttctgtta attatctttg gtctaatggt gcgaccagtc
    10381 aaacaatcac taatcttgcg ccgggcacat attcagttac agtttctgat aatatttgcc
    10441 aaaaagtaag ttcagttgaa gttgtatctc caggcccaat aaatatcatt ccaaccctga
    10501 ttaatcctgc ttgtgatgac aacaatggtt caatcagtat taatgtcaat ggaggaacag
    10561 caggctatac ttatttgtgg tctaatggag ccactacaca gaatatttct gccttaaacg
    10621 cgggaaatta taccgttaca gtcctggatg ctcacaattg ttcttcacaa caatctttta
    10681 ccttgaccgc tcaggacaat caggcaccta ccttattaaa tgataagatt accctatacc
    10741 tggatactaa tggcaatgca gaactcacta ccattccgac atcatatctc tttgaagata
    10801 attgtggaac agtatctgtt gtattccaga atacatcatt cgattgtgat aagttgggga
    10861 agaataacat aactgctaca ggcaccgatg aagccggcaa ctcaatacaa gtaaatttag
    10921 agataaccgt cattgattct atcaagccat tcattcaatg tccatcagat tttactatcg
    10981 ggatttgtca gggggctccg gtatgggacg atataatata ttctgataat tgtcaggttg
    11041 tcactattca gcaaactgca ggatatgcac ctggcactat cttcccgata ggcgttactg
    11101 aagtagctta tcaggtaaca gatttgtctg gtaatcaaaa ttcttgtagt ttctcaattc
    11161 atgtagcaga aggattggct ctcaatgtca gtaagacgga tatggcatgc aacaatgttg
    11221 caaatgggag tgctactctg gatactaaag gtttcgtaga accatacact attttatgga
    11281 gtaccggcgc aaatacccca tccataacta atttggggcc cggtgattat tcagttacag
    11341 taaccgacgg gaacggatgc gttgctactc aaactgtcac aataattaat cctgcaccac
    11401 ttgaaatgga agttggttcg gtaacacaag catcctctga aactgctgct gacggagcaa
    11461 taagtatttc tgtctcaggt ggcaccccgg cttatagttt ctcctggact aagaatggag
    11521 aatttatttc ttcagatcaa aatccttccg gattaactca tggggtctat aaggttgaaa
    11581 tcaaagataa gaatggatgt gtcattattg gtgctcagat aactgttgga tttaaacttg
    11641 gtaatagtaa ttgggataaa ggcactgtat tggtatatcc caatcctgct gatcagagat
    11701 tagtagttgc gtctgcttcc atggaagatt ccatgcttga gatatatgat attttggggc
    11761 gtaaaattga ccttgatttt tctaaaggat ttgagaagat atttgttaat actgaaagtt
    11821 tgagcaatgg atcatacata ctgcgcctcg aaagtggtaa tcagtggtat gttaccaagt
    11881 ttgtcatcat acattgatcc aagttcatgg tagatagatt aagcccggca actcaggtta
    11941 tcgggctttt gttattaaat gactagggat gagaacgcgt taatacaatg caacgattgt
    12001 tgaacatata tatcggtgat tctaaagaga aaggtcgcgg agtattcact tcattgccaa
    12061 taaaggcagg agatgtcatt gagatctgcc ctgtagtggt tatcccaaag gatgaaatcg
    12121 aactcataga ccggactgtg ctctatgact attatttcct ttgggaggaa ggctgtgccg
    12181 ccatggcttt gggttatgga tcactttaca accattccta ttctccaaat gttgaatata
    12241 tcatggattt tgaggattct gtgataatta tcaaatcact tcgggatata gcttcgaatg
    12301 aggaattgac cttcaattat aatggcgatc cttttgataa agaaagaata tggtttgatc
    12361 caatgtaaaa ttgggcttta cccaaaattt tatttgcccc acattgccaa ttattcgaaa
    12421 aatagtctta cctttgcgcc gctaaaaata ttgtttaacc attaaaaaca tttagattct
    12481 atgtcagata acgagaatct gcttcaggag tcacccgaat caacacctgc agctcccaaa
    12541 aaaacagcac atgatgcttt cgattggaca ataagcaaaa aaaaccacat tgcttattct
    12601 gcagaagaaa ctcaaaaata tcttgaggat tacgaaaaaa cactgaccct tatcaaagat
    12661 aatgagatag taaaaggtct tgtgaaatct attagcagtg gagatgtcgt attggatatt
    12721 aattacaaat cagatggtat tgtttctctt tcggaattcc gtgacacacc tgaccttaag
    12781 gttggtgatt atgtagatgt ctatgtagaa agtcaggaag atgcaaaagg gcagttggtg
    12841 ctttccagaa agaaagctaa attattgatt gcctgggata ctattgttga ttcctttaac
    12901 aatggtacta tcatcaaagg tactgttatt tccaagacca agggtggact tattgttgat
    12961 gcctatggcc ttgagacctt cctgcccggc tctcagattg acatcaagcc tattattgac
    13021 tatgatgctt atgttggtaa gaccatggaa ttcaaggtag ttaaaattaa cgagactatc
    13081 aagaacgcgg ttgtttcaca taaggcactt attgagagcg atatcgctga acagcgtgaa
    13141 cacattattg ccggtcttga aaaaggacag gttcttgaag gtgtcgttaa gaatatcacc
    13201 gattttggtg cattcatgga tcttggtggt gtagatggtc tcctttatat cactgatatt
    13261 tcatggggta ggatcaacca tcctgaagag gttctccaaa tgaatgataa aattaatgtc
    13321 gttgttttgg actttgatga taataaaaaa cgtatctctc tcggtctgaa gcagttgact
    13381 cctcatcctt gggacgttct ggctatggac atttcagaag gctctattgt taaaggaaag
    13441 atcgtcaatg tcgaagacta tggtgcattc cttgaaataa gcccgggcgt tgaaggattg
    13501 attcacgttt ctgaagtttc ctggagtaac cagccggtag ttgcgcgtga tttcttcaag
    13561 ttggatcagg agtttgaagc aaaggtagtt actatcgaca gagatgaaag gaaaatgtcc
    13621 ctgagcttga aacaacttca ggaagatcct tggacagaaa ttgaaaacaa gtatccagtc
    13681 aatagccgac ataccggtac tgtgaagaat ttaactccat atggcgtttt cgttgagatt
    13741 gacaatggta ttggaggaat ggtccatatt tctgaccttt catggacaaa acgattctct
    13801 catccttcag aatttactaa agttggtgat cagattgagg ttgtcatcct cgaaattgat
    13861 aaggctaata gaaagttgtc tttgggacat aagcagatag aagaaaatcc ttgggatgca
    13921 tttgaagctg tattccctgt agggtcttat catgaagcaa ccgtaatcag aagggacgat
    13981 aaaggtgcaa cagttcagct gccttatgga ttggaagctt ttgctcctat caagcacctg
    14041 aagaaagaag atggatctac tgcagaagtt gaggaatcac ttactgtcaa ggtaattgaa
    14101 ttcaatagag acgataagcg tatccttgtt tctcatagcc gttatctgga ggatatcaaa
    14161 aaggaagctg ataatttgat caagcgtgac aaagaatctg ctgataatga aacaaagtca
    14221 gcaattaaga cgcaacaagc caaattagag aaaacgaccc tgggtgatat tgatgctttt
    14281 tctcagttaa agcaacagtt tgacgaagcc ggtgaaaaga aagcagaaga agctaaggaa
    14341 gagttagctc ttaaggctaa gaaatctact aaggcaacta aagcaaaaga agtcgttgct
    14401 gaagaaactg ctgctacaga gggaggtgat acgcccgaag attctgagaa aggtgaagat
    14461 gcttgatttt ttgtgaaaat catttagatt cagctaaata atcaaaagcc gtgttttgaa
    14521 attcttttca agcacggctt ttcaattatg taattgtttg tcaatgtgag aaagagaagt
    14581 ttatttcaac atccctgata tattttcgtt atctatcttt gaatcgttgt atttttatcg
    14641 cttaatcgct taaggaccta aatggctgac aataatttct tgaaaaagtt gaagagtata
    14701 ctcgcatcaa tacgaattag gagtaataaa attgatattt tccttgaaaa aaggcccggt
    14761 ttaaattttg ttttaaaagc cggtatcagt gcacttctca caggctttat ttgcttgttc
    14821 gttttaatct tctcagcgcg aataggtgtt tttggtggaa tgcctacaga cgacgaactg
    14881 aaagaaatta gtaatcctga aggaactgaa ttatttgcag atgatggtgt tacgctttta
    14941 ggtaggtatt acattgaaaa cagatctaat atcacttacg attctttgcc ggagaatttg
    15001 attcatgcct tgatagctac ggaagatatc agatactatg aacacaatgg tattgattgg
    15061 aaatcctggg cgcgagtgtt gttcagaaca attttaatgc aggaaagtgc atcaggaggg
    15121 ggtagcacaa ttagtcagca attggctaaa aatttattta aaagaaggac ttattggcgt
    15181 ggcactaccc ttatcaataa aatgcgtgag attattatcg ctcaacgatt ggagagtatg
    15241 tacactaaag aacaattgct gacgctttat tttaatactg tttcctttgg tggtaatgtc
    15301 tacggtattg acattgcttc aaagcaatac tttaaaactt ctcccagatt tttaaagacg
    15361 gaggaggcag ctactttggt tggtatgctt aaagccacca ccaagtataa tcccctcaat
    15421 aatattgatc ttgccaagaa aaggcgaaat actgttttaa acaaaatgga aaagtatcaa
    15481 tatatcaccc aaaaacaact ggattcattg tctgcactgg atattaagtt gaattatgtc
    15541 aaggagacac atacggaagg tattgcaact tattttcgcg aatttgcaag attggatctt
    15601 gataaacaat tggctcattt gaaaaagaat gacggaagca gctacaatct atatacggac
    15661 ggcttgaaag tttatacttc aattaatatt ccaatgcaga attatgctga atctgcagtg
    15721 gccgatcaaa tgaaagaact tcaggagcta tattatcaac aaatgcgctg gattaaacta
    15781 ccttggaaag acaccgcttt tctccataaa gcaatcatga ataccgaccg gtataaatca
    15841 atggttacag ccggtttgtc acaacattac attgacagtg tatttaatgt tccatatgaa
    15901 atggaagttt atagtccgca gggtccaaag acaatgaaaa tgagccctaa ggattctctg
    15961 atccattatc ttcagtttct gacggcggga tttatggccg ccgacccttc taccggtttt
    16021 atcaaggctt gggtaggggg cattgattac aagtattttc aatacgatca tgtgaaagct
    16081 aagcgacacg tcggttctac catgaagccg attgtgtatg cagctgcttt ggaaaaagga
    16141 cttgaccctt gcgcgtatta cgataatcga caaaagacgt atcctcagtt tgagaattgg
    16201 tctcctaaga attttgaagg cggatatggt ggcgaatatt ccatggcagg cgctttggcc
    16261 aaatcactca attgtgcaac agtggatatt atgcttaaaa ccggtattga caccgtggtt
    16321 gattttgcaa aaagaattgg cgtaacatct actattcctt atgaaccatc tgttagctta
    16381 ggagctgttg atatttcttt atatgacatg ataactgcct attctactat cattgataga
    16441 gggcaaaagc cagctctgac ttatatcttg aaggttgttg attctgaagg aaatgtattg
    16501 attgacaata caacaccgga agcccaggaa aaagtacagg taatgaatcc tgaaaatgcg
    16561 gatatcatgc gatccatgat gcagggggtt attaatcatg gtactggtgg ttctatcaag
    16621 tggagatatt ccatcacaga agaccttgcc ggaaaaaccg gaacaagtca ggactgtagt
    16681 gatggttggt ttattgcgtc aaatccgaaa ttgattgccg gggcatgggt aggtggtgaa
    16741 tttcctgaaa tacatcttac gggtaatttt accggtgctt ttaccgcctt accgatagtt
    16801 ggaatgtttt tgagaagtgt tgaaaaagat aagtccttga atgactatgt caaggagaaa
    16861 tttcctgaat tatcacagga agcaaaaaat cgtttgaatt gtccctattc tcactatgtc
    16921 gctgactcct cggctatcga ttctaccgga cttgactcgc tcggtaatcc gataataacc
    16981 gatgatgatt tccatgagca ggatgatgag tcgccaaaag tagataccac taagaagaag
    17041 aagtttttcg aagggctttt taatagaaaa gaaaagaaaa aggttgataa cgagaacaac
    17101 tgatgtttgt ttctcaagaa attgaatcga aatggaaatt gtcgaaacgt ctgggatatt
    17161 gattgaaact ttgccgggag ctgatatacc tcctgttgaa tcaactttga ttgaattgta
    17221 taaaaaattg gtttatccca ggttgattga agaaaaaatg cttaaccttc tccggcaggg
    17281 taaaataagc aaatggtttt ctggaattgg ccaggaagct atttctgttg gcgtaaccgc
    17341 tgccttggat gaagatgagt atatttttcc aatgcaccgt aatctcggag tattcacatc
    17401 aagaaatatg cctcttgaaa ccctgttcag tcaatggcaa ggaactgaag gaggatattc
    17461 caaagggcgc gaacgttctt tccattttgg ttccgtccct catcacattg ttggtatgat
    17521 ctctcactta ggcccgcaat tatcttatgc ttccggagtc gctttgggcc ataaacttgc
    17581 tggaaataga aaagttactg ctgtattttc aggtgagggt ggaacaagtg agggagaatt
    17641 tcatgaagca ttgaacgtag cagccgtttg gagtcttccg gtaatttttg ttattgaaaa
    17701 taatgggtat ggactttcca ctccggtcaa tgaacaatat agatgtgaac gcatcagcga
    17761 caaagggaaa ggatatggca tgcgtgcaat tacaattgac ggtaatgata ttgtaaatgt
    17821 atttaatacg gtcagaatac tttcggaaga aatcaaagct gatccgcatc cggttctttt
    17881 ggaatgtgtt actttcagaa tgcggggaca tgaagaagct tccggaatca aatatgttcc
    17941 aaaggaattg ttggaatatt ggtcaacaca ggatcccatc ctgagatatg aaaaatatct
    18001 tttggaaagg aatctgatga atgaaaatga tttcctttca attagatcca aatttaagga
    18061 agaaatcaat gaagctgtaa ataatgcgct cagtcactca cccattgtgc catccactca
    18121 aactgagctg gacgatgttt ttgctccctt tgaatacaat gaaatcagtg gaggtcctgt
    18181 tgagactgaa ttgaggatga ttgatgcgat ctcagatgga ttgaatgagg caatgaaaaa
    18241 acattccaat ctgattttaa tgggccagga tattgctgag tatggtggtg tctttaagat
    18301 tacagatgga tttttagaaa ggtatgggaa ggatagagtc agaaatactc ctctttgtga
    18361 aagcgcaata atcggaacag ctttaggcct tagtcttaag ggttataaag ctatggttga
    18421 gatgcagttt gctgattttg ttacttgcgg gttcaaccag attgttaata atcttgccaa
    18481 actccattat cggtggggtc aacatgccga tgtggttatc agaatgccta caggcggtgg
    18541 agtaggagcc ggaccttttc attcccaaag caatgaagca tggtttaccc atgtaccggg
    18601 ccttaaggtg gcctatccat ccaatcctta tgatgccaag ggtcttttac tgactgcatt
    18661 tgaggataca aaccctgtat tattctttga acataaggca ttataccgat ctcaaatcag
    18721 gatggttccg gaaggttatt acaacttgcc atttggaaaa gctgccattg caagaaaggg
    18781 tgaaggcatg acaatcatta cgtatggtat gggtgttcat tg
//
LOCUS       DAOQMC010000013        17656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1127_length_17656_cov_5.531674, whole genome shotgun sequence.
ACCESSION   DAOQMC010000013 DAOQMC010000000
VERSION     DAOQMC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1127_length_17656_cov_5.531674"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..297
                     /locus_tag="PKV31_01105"
     CDS             100..297
                     /locus_tag="PKV31_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91475.1"
                     /translation="MRYLNNSAESAEVLPDTCPDSYATRALLCPGSETIRGSGMPRLQ
                     SYRPMKLVYNECTFGIRKNTG"
     gene            complement(381..1784)
                     /locus_tag="PKV31_01110"
     CDS             complement(381..1784)
                     /locus_tag="PKV31_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNB91476.1"
                     /translation="MKSKKTSVIVLLAILFLVSILHVVDNFFSANVDKGILSVSHLLL
                     SLSFIYFAIMRRNLTTWILISMVIGALIGHEFPVVGQNMRVLSQVFLKLIKTIIAPIL
                     FSTLVVGIAGHSNLRQVGRMGWKSIVYFEIVTTFALFIGLIAINISKAGVGIQMPPDI
                     HETLPEVQQQTWQDIILHIFPENIARSIYHAEVLPIVVFSVLFGIGLAMVDEKKKRPM
                     LEFCESLAETMFKFTNIIMYFAPVGVGAAIAYTVGHMGLGILVNLAQLLMTLYVALIL
                     FILLVLLPVALIAKVPIVNFLKAVLEPMSIAFATTSSESALPKAMQAMERLGVPRKIV
                     AFVIPTGYSFNLDGTTLYLSLASIFVAQAAGIHMDIPTQLFLVFTLMLTSKGVAGVPR
                     ASLVILLATAAQFNLPTWPILIILGIDELMDMARTATNVLGNCLASCVVARWEGEFEP
                     RDPTIPLESELRQPQSA"
     gene            2035..3633
                     /gene="pckA"
                     /locus_tag="PKV31_01115"
     CDS             2035..3633
                     /gene="pckA"
                     /locus_tag="PKV31_01115"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNB91477.1"
                     /translation="MSTTKNTDPRSICKTTGIENPARVYYNATPEQLIEETILRGQGL
                     LSDKGALVIKTGEFTGRSPKDKFIVTDAKTENEVNWGGFNTRFDADKFEKLEKKMLRY
                     FDGKDVFIRDNYVCADENYRLNVRTITEYPWSNMFANNMFLRPSAADTSEPEWTILCA
                     PEFYADPAEDGTRQHNFSIVDFTRKKVLIGGSGYTGEIKKGIFSVLNFLLPVDKGVFP
                     MHCSANTGRQGDTAIFFGLSGTGKTTLSADPERNLIGDDEHGWTNEGVFNFEGGCYAK
                     TVHLTEENEPEIYHAIKHGAILENMKFVEGSRTPDFEDVSITENTRVSYPMHHIGNAL
                     PVSKGPHPANIFFLTCDAYGVLPPISRLTKGQAMYHFISGYTAKVAGTEAGVKEPQNT
                     FSACFGAPFLPLHPARYAAMLGEKMEKFSTNVWLVNTGWTGGPYGVGSRMKLKYTRAM
                     ISQAIKGKLNNVAYHKHEIFGLNIPVSCPDVPSEILDPRNTWSDKEAYDRTAKKLAKD
                     FNDNFSKFADLASQEIRDAAPKHD"
     gene            3747..4235
                     /locus_tag="PKV31_01120"
     CDS             3747..4235
                     /locus_tag="PKV31_01120"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HNB91478.1"
                     /translation="MELFKIIVDIKKEDIIALFKYHQKQKRVRTNLIYVGLIILFTFF
                     FKSVKVEIGEAVLLAAILVLVFAFLNPRLGRLRYLKQFEANKNLQQPLEYHIFADRYM
                     VKTSIAESEVQWSTRYNISQNDDYIFIFHTEEMTSMLPKRCMTDDQLTFFGELIKRFG
                     KA"
     gene            4649..6487
                     /locus_tag="PKV31_01125"
     CDS             4649..6487
                     /locus_tag="PKV31_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB91479.1"
                     /translation="MNLFLKLLRFSRPYHHYIPEYIIYVFFFTIFGLLNFVMIIPLLD
                     FLFQENPISEPSSFPAFSFSASYLQDLFKYYAWQFSAGENQKFKLLAYITLILLTVTS
                     LKNFFGFMSQKVLARMRVNLVRKIRNQLFEKLTNTSVGFYHNRQKGHLLSTLSNDVGE
                     IENTVVTSIQVLFRDPFAIILTFTALFIISRELTFFTLLFFPLSGIAINKISRGLRKK
                     SVESQSLLGSLMSYTDEMISGNRVIKVFNAEGFVQRQYRMLNDKYTRVAKRIATNKEL
                     ASPLSEVLGVGVIAVIIIYGGHLVLAGQGELTASMFLGYLGLYFSILNPAKNIGQAYA
                     NLQRGMVSGARVFEILEQKDTIQNRPGAVAIDGFKEGIRFEHVGFRYSGETVVLNDFC
                     LNIPKGKTIALVGESGAGKSTVADLIPRFYDVTNGRVLMDGMDIRDITMDSLRKQMSM
                     VTQEAILFNDTVFNNIAFGMDDVSLERVMEAAKVANAHAFIEQLEDGYQTIIGDRGMR
                     LSGGQRQRLTIARAILKDAPIIIMDEATSALDTESERLVQDAINRLTHNRTGIIIAHR
                     LSTISHADEIIVLQRGSIAERGTHQELMQKGGYYRKLVEMQQLEKK"
     gene            6842..8155
                     /locus_tag="PKV31_01130"
     CDS             6842..8155
                     /locus_tag="PKV31_01130"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HNB91480.1"
                     /translation="MDPLKELFKTKAFPVRDELKTVIKEHGEMKIAEVKISQLVGGMR
                     SIKSMLWETSLLDAEEGIRFRGYSIPQLIELLPKIEGDSEPLPEGLFWLMLTGDIPSR
                     DQVEWLSKEWERRSQVPDEVFAILDACGNDVHPMTMFSIGVLAMQPYSIFAKRYDEGF
                     SKADYWDATYEDTMNLIARLPHIAAYIYRKVFRNNEHIPSKKGLDWAANFAHMLGFER
                     DDFKSLMRLYLTIHADHEGGNASAHATHLVGSTLSDAYLSLSGGLNALAGPLHGLANQ
                     EVVNWINEMVEKIGTKKPTKEQIADYVNTTLAAGQVVPGYGHAVLRKPDPRFMEQKRF
                     AERYMPDDDYVNIVWKVFDVVPEILRGLGKVTNPWPNVDAHSGSILIHYGLTESSFYT
                     VLFGVSRAMGVLAQLCWSRALGLPIERPKSITFDNVKEFINNQKS"
     gene            8167..8559
                     /gene="gcvH"
                     /locus_tag="PKV31_01135"
     CDS             8167..8559
                     /gene="gcvH"
                     /locus_tag="PKV31_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNB91481.1"
                     /translation="MSNVPKELKYSKEHEWIRVDGNIGTVGITDFAQGELGDLVYVDI
                     DTVGQTIAKDEVFGAVEAVKTTSDLFLPVTGKILEVNPAITESGDDDPSLINSSPYDK
                     GWIVKIEIANPSELDSLLDSDAYEALIG"
     gene            8559..8966
                     /locus_tag="PKV31_01140"
     CDS             8559..8966
                     /locus_tag="PKV31_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNB91482.1"
                     /translation="MAKLHIDRHRLFRVLARVWIITLTILMLLPGKSIPVVRWALNIP
                     IDKTIHFLSFFFLAAGFLLGYRWEDRSKRRILFVELLVYASIMEVIQHLLQPSRAFEM
                     ADMLANILGIITAFIGYYVLGKFIKKLTLNPPN"
     gene            9126..12752
                     /locus_tag="PKV31_01145"
     CDS             9126..12752
                     /locus_tag="PKV31_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB91483.1"
                     /translation="MKRFFLLFLSLSLFSKISAQQIGWQPVSKEVAMGRSWASDENVP
                     LENLFKVDFNELNQSLLNIPVSAKVGFDMEMPMPDGKTQLYTVVSDPVMEADLQARYP
                     NIRTYKVISKETGGLAGRIGTSPEGFYGVLDINGKEVLINKVNRNDRSLYSVFRLVDG
                     INAVDMFKPMACGADFAEVKKVEASSMEDKIETRAVEVKMQHFRVGIATTSSFAAEIG
                     LTTVEQVLARVTQVINQVNLRYNKDHAMHLDLISNTTNLFNMTPEDDFFKQQNNGGQL
                     LGPSQEFFQMKLANKDYDFGQTWTQYCVDVGGVVSGSACNNQSKARGVSCGPSNVSYF
                     LQTVKHEMGHQFSASHTFNSCNGSTQQASQGAYEPGSGSTIMAYPGACGPDNIAGGAD
                     DYFHVISILHVRGHHQAYPECGTFAEDVNHMPEITLPKYNSDLLTIPKLTPYELIGSA
                     TDHNQDKLYYIWEEFDQGNGEPLGTNFESGPLNRTYPPLTNNSTRLIPRLNNIVNDVF
                     DIADRMPEATREMNWKFVVRDYNPNAGAVAIADFKFRVDSNAGPFKFSFPNKNTDSVL
                     TVGQYFEVKWDVANTDKEPVNTKFVDIFYSTDGGVNYKDTLVIGTPNDGAEYVTIPQK
                     AQNSRFKIKGHGSIFLDMSRKTLRVVEPDSAGFSYDVYPHNALLCPFEPQNFLIKGIS
                     WKGFDKPIKVELVDGWPSNTVIKLDKEIMNPGETLTLSIDTRDVTDAGFYTIRMIATA
                     EGQDTIWRYMEVEILPSKLEFTGAVNPLPGNTNESQVPSFAWNIIPGADRYEFQLASD
                     SKFENILHTYTGTAIAIEPQVVLPPGAIYYWRVRASNRCYDGPWSEYNTFQTILFECN
                     TINALGLPKAISSNVTAPPVDVLFDISNSTGVVADINVKNIDISHSDISKLKISARSP
                     SGKEINLMTGRCAGISGLYTTFDDVSPLEINCGLIKANKTFKPEEELSAFNGEPVQGN
                     WSIRVTTIRGGSSGKINNIDFDLCASVTTPPIIKVNNNLFKVNSDHSQVLENKDLLYT
                     CENTGDGNIWYIMLSEPVHGYFTLYGQEIQKGDRFTQGHLNDWGVQYVPYDSEYEGTD
                     KVDFLVRNDKFAYLAPEQFNIDLSKTHTVSTKPINKDISKLIKFYPNPTNGEVNIDLS
                     DIKILETGKLTVTDIAGKVVMQKPIPANTDRVVLDLGRMSAGVYFAGIYSGNYHAMQK
                     IIKQ"
     gene            12874..16035
                     /locus_tag="PKV31_01150"
     CDS             12874..16035
                     /locus_tag="PKV31_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB91484.1"
                     /translation="MKIIRLLAFNLLFISLYTTSRAQININWQTANPSSEQKAEISSM
                     LLLPQDARFYKASFLHTTLESVSGSEVVQVTLPIGMDGAEVKVHFVYDPVMGARDQQK
                     FKEIRTFKAFSTEGTPISGRAGISPTGFYGVFELNGKQIMIRPGAVEQDMYVVYDLGR
                     KLSMLDFSLLAGCGTETHLNEIKRNETVNLRSDDKRMRNFTIAISCTSGYADQVGRSE
                     GQVMASVVEVLNLLNYRYNVDFGIKLNLLDGTSQLFNLDPHTDYFVNETKGLELLQQN
                     QDFLDSLIDNSSYDLAQVFTRGCSDVGGVVWGRTCVSDTKARGVSCRTNDSDYFFTTF
                     KHEVGHQFSGGHTFNACNGSTQYNPFSSFEPGAGSTILSYGDNCGSDNVGERVDYFHV
                     ANIIEVNRYVKEIENKCGTWGAEINHTPVGYLSMPEDKTIPILTPFELEGGATDDDKN
                     LTFNWEQYDLGEGEPLGTNFETGPLFVSQAPGGVGHRLFPSLSTINSGIISNRERLPE
                     VSREMNFKMTVRDNNPNVGAQAIVSYKFDVTDQAGPFKVTFPDAQTDTSFTLGQYVEI
                     TWDVANTDQFPVSCKNVNILFSTTNGNTWSDTLVANTPNDGRESVMMPRTSGRARFKV
                     KAADNIFLDLSGKPLKIVQPSEAGYSLDILPHDISLCKNSIYTLTVKSVSWKAFNSPV
                     KISVFDSGHPAFKVIPLKSEFLPGETMTLDVDVPDVIDAGTYQIRFMATSDQGDTLFR
                     TVTLTIADNDQNISGTISPVANDQYVTLHPVFAWDPVENTHGYQLQLATDPAFSSLIL
                     NVSIDSEEYLSEIELNKATIYYWRVRSLGECGYGRWTAMSIFRTESELSNSALHQINN
                     KILKVKTNEKKPALHEDLYYVSELNQNESLKYTLLSEPEHGALYLDNARLKKGDTFTQ
                     ENVDAGKFVFSAEELDYQGGDGFAFLVSDISNLFIGPENFIIDIRESYPSGTSHINMD
                     AMVDLVPNPTSGLIDLKCVGMDLKEDVEIQILDIRGVSVMRPFKAQLNLGNRIDLTSL
                     SSGVYLIVVQSGNYIAKKRLVKI"
     gene            16111..16899
                     /locus_tag="PKV31_01155"
     CDS             16111..16899
                     /locus_tag="PKV31_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNB91485.1"
                     /translation="MFRSKYFWTHVGLMILAVGLLTGLIFFFLGLYTHHGEKIPISGL
                     NGLQVELAKRQAEEDGFEVVVTDSVFIVGQKGGIVLAQNPEKGTFAKEGRKIYVTLSK
                     YMPDMVPIGSLPALYGKNFELKRKVLMEGYEIQSAVVGQLYDAGEPGCILRVIYNNDT
                     IIDARGVRDNVNLPKGAKLKFIVSTNQGGQMEVPDLICQEFDEARFSLGNNFELSGRS
                     GTSGKYIVAQEPAYTPGVKIDRGTTIIVDLSDSRPPGCPPDEDE"
     gene            16944..>17656
                     /locus_tag="PKV31_01160"
     CDS             16944..>17656
                     /locus_tag="PKV31_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91486.1"
                     /translation="MNRFSLRIFFFLWILLTGVSSGAQVVYYGLTENPVLFPEKQVSG
                     QRNLISDSRVVVIPSDSQTLCINRPEIGTFDTLILDQASVNPGIDVKITENCIVFIAE
                     QNAELGDYEYTFDYSSFSGNSLKYRVALSVVEPRGLPFFDDFAYNDKYPDQRRWVDKN
                     VFINNTMGDNPPSVGVATFDGLNSNGSPYGGGFGRADYLTSTFFDLSGVQHSEPVFLS
                     YYLQPKGKTYNHQLRDSIEL"
BASE COUNT         5237 a         3538 c         4023 g         4858 t
ORIGIN      
        1 ctgctaccgc tacaacaggc accgcatgaa aggcgaaatc ctggatgaca tcatcgccag
       61 aatggtcagg cataggccga tgtcttgtaa aaaggcagca tgagatattt gaacaattct
      121 gctgaaagtg ccgaagtatt gccggatact tgtccggaca gctatgctac tagagctctg
      181 ctctgtcccg ggagtgaaac aatccggggt tccgggatgc cgaggttaca gtcgtataga
      241 ccaatgaaat tggtctataa cgaatgtacc ttcggtatta gaaaaaatac aggttgacaa
      301 ggctaaaatc tctataaagc ttttgtcact tatttttctt gtgatatact tgggctgtcc
      361 tgcctttcat gggaaataat tcaggcggac tgaggctgcc taagctccga ttccaatgga
      421 attgtcggat cccgtggctc aaattctcct tcccatcgtg ccacgacaca actggcgagg
      481 cagttaccaa gcacattggt ggctgtacgt gccatatcca tcaactcatc aatcccgagg
      541 ataataagta tgggccatgt tggcagatta aattgggcgg cagtcgccag taaaataacc
      601 agtgatgccc gtggcactcc ggctacaccc ttactggtaa gcatcagtgt gaagacaaga
      661 aacagttgtg tcgggatatc catgtgaatc ccggctgcct gcgctacaaa aatggacgca
      721 agggataaat aaagagtcgt cccatcaaga ttaaaactat agccggtggg tatcacaaaa
      781 gctacaatct ttctaggtac acccaaacgc tccatagcct gcatggcctt gggaagtgcc
      841 gattctgaag atgtggtggc aaaggcaatt gacattggtt caaggaccgc cttaagaaaa
      901 ttgactatcg gcaccttggc aatcaatgca acaggaagaa gtacaagcag gatgaacaag
      961 attaatgcta cataaagtgt cattaaaagt tgcgccaaat tgaccaatat ccccaatccc
     1021 atgtgcccga cagtatatgc tattgccgca ccaaccccaa ctggggcgaa atacatgatg
     1081 atattagtaa acttgaacat tgtttcggca aggctctcac aaaattccag cataggcctc
     1141 tttttcttct catccaccat agccagaccg attccaaaaa gcacactaaa gacaacgatt
     1201 ggtaagactt ctgcatgata aatagaacga gctatgttct caggaaaaat atgcagaata
     1261 atatcctgcc aggtttgttg ttgaacttcc ggcaatgtct cgtgaatatc tgggggcatt
     1321 tgaataccaa ctccggcctt tgatatgttg atagcgatta atcctataaa gagggcaaaa
     1381 gtagtaacaa tctcaaagta aacgatagac ttccacccca tccgaccaac ttgacgaagg
     1441 ttggaatgcc cggcaattcc tacaaccaac gtcgagaaca gaattggtgc tatgatagtc
     1501 tttatcaatt tcaagaatac ttggctaagg acacgcatgt tttgacccac aaccggaaat
     1561 tcatgcccaa tcaacgcccc gatgaccatc gatatcagta tccacgtcgt cagattgcga
     1621 cgcataatgg caaaatagat aaaacttaag ctcaataaaa gatgggatac agataagatt
     1681 cccttatcga cattggcact gaagaaatta tctaccacat gcaagatgct gactaaaaat
     1741 agaattgcta acagtactat aacacttgtt ttttttgact tcatccgtat gtttttcaga
     1801 tcttaaaaaa aattgccaac aatttaatta aaacggttaa aattaattat tttgtggttc
     1861 gcgtctcgat tccttttgat ttttttaata aaaggaaaat tcattcaaaa atgatgcaat
     1921 gtaattctat tttttcaaaa caaataatct taattacatc aaaattccaa aatttatttt
     1981 gaaccttcgg ccttttcttc taaatttgtc gtctttaaat ttaaaattct ctctatgagc
     2041 actaccaaaa ataccgatcc cagatccatt tgtaaaacta ccggcattga aaatccggca
     2101 agagtttatt ataatgccac tccggaacaa ttgattgaag aaaccatcct tcgaggtcag
     2161 ggcctgcttt ctgataaagg cgcgttggtc attaaaaccg gcgaattcac cgggcgatcg
     2221 ccaaaagata aatttattgt cacggatgct aaaacggaaa atgaagtaaa ttggggcggc
     2281 tttaacactc gctttgatgc agataaattc gaaaaactgg agaaaaagat gttgcggtat
     2341 ttcgatggta aggacgtatt cattcgagac aattatgttt gtgcggatga gaattacaga
     2401 cttaatgtcc gtacgattac tgagtatcct tggagcaata tgtttgccaa taacatgttt
     2461 ctcagacctt cagcagcaga cacttcagaa ccggaatgga cgattctttg tgcccctgaa
     2521 ttttatgctg atccggcaga agatggtact cgacagcata atttttcaat tgttgatttt
     2581 acacgaaaaa aagttttaat agggggctca ggttataccg gagaaatcaa aaaaggaatt
     2641 ttttctgtct tgaatttcct tctgccggtt gataagggag tattcccgat gcattgctcc
     2701 gccaataccg gtcgccaagg agacacggca atattcttcg gacttagcgg aacaggtaag
     2761 acaaccctct ctgccgaccc tgaacgaaat ctgataggcg acgatgaaca cggatggacc
     2821 aatgaaggag tatttaactt cgagggtgga tgttatgcca agacagtaca cctgactgag
     2881 gagaatgagc ccgaaatata ccatgcaatc aaacatggcg ccattcttga aaacatgaaa
     2941 tttgtagaag gcagccgcac tcctgatttt gaagatgttt cgattactga aaacacacgt
     3001 gtttcctatc ctatgcatca tatcggcaat gcacttccgg tttcaaaagg cccccatcct
     3061 gccaatattt tctttttgac ctgtgatgcg tatggtgtgc ttcctccgat ttcaagattg
     3121 acaaaaggac aggctatgta tcactttatt tcaggatata ccgccaaggt tgcagggaca
     3181 gaagcgggcg tcaaagagcc tcaaaacact ttttctgcct gctttggagc cccgttcctt
     3241 ccgcttcatc cggcaagata tgctgctatg cttggtgaaa agatggaaaa attcagtacc
     3301 aatgtttggc tggtaaatac aggctggaca ggcggaccct atggtgtcgg cagtaggatg
     3361 aaattaaaat atacccgagc gatgatttct caggctatca aaggcaaact aaataatgta
     3421 gcttatcata aacatgaaat atttggcctc aatattccgg tatcttgccc ggatgtgccg
     3481 tctgagatcc tggatccacg aaatacatgg tcagataagg aagcatatga cagaaccgct
     3541 aagaagttgg cgaaagactt caacgacaac ttctccaagt ttgcggatct ggctagccag
     3601 gagattagag atgcagcacc aaagcacgac tgatacttta atgaaatata atcttgaaac
     3661 gccacaactt gtctgtggcg tttttttatt taaatggcca attattctac ggataatcgt
     3721 agacgaatct tttcaaatat aaatggatgg aattatttaa aatcattgtt gatatcaaga
     3781 aagaagacat catcgccctt ttcaaatatc atcagaaaca aaagcgagtc cgcactaacc
     3841 tgatatacgt ggggctgatc attcttttta cctttttctt taaatctgta aaagtggaga
     3901 ttggagaggc cgtgctgctg gctgccattt tggttcttgt attcgcattt cttaaccctc
     3961 gactgggccg attacgttat ttgaaacagt ttgaagcaaa caaaaacctt cagcaacctc
     4021 ttgaatatca tatttttgca gaccgatata tggtaaaaac ttctatcgct gagtctgaag
     4081 tacagtggtc gaccaggtat aatatcagcc agaacgatga ttatatcttt atttttcata
     4141 cagaggaaat gacatccatg ctgccaaaaa ggtgcatgac cgatgaccaa ctcacttttt
     4201 ttggagaatt aatcaaaagg tttggaaaag cttgagcgta tatagatgcc gtattttgta
     4261 aattttgtga agggcttgtt tttgaggatg ttattagaat tgaattttat cacgaagtag
     4321 gtagatgcct aataggtgat aatgtgtaag ccacaacact tcagaatcta tatactctaa
     4381 caaaagattt aaaagtgacg gaatttttta gcagattttt accaattgag ttctggtgta
     4441 tatttccgtc tattaatttc ctacccactt cgtttttcac caccccactt caaatttcgg
     4501 ttgtattttg acctctggaa gcccgaagta cagccttttt ggcaggtctt ttttgccgtt
     4561 ctgtttttga aatgtaacga atgacgacaa attagtattt ttacgcctac tattctgaat
     4621 attactattc tgatggaata aatagcggat gaatttattt ttaaaactat tgagattttc
     4681 ccggccgtat catcattata ttccggagta cattatctat gttttctttt ttacgatatt
     4741 cggcttgttg aattttgtca tgattatccc gctgctggat ttcttgttcc aggaaaatcc
     4801 tatcagtgaa ccatcgtctt ttccggcatt ttcattcagt gcgtcctatc ttcaggattt
     4861 attcaaatat tatgcctggc agttcagtgc tggcgaaaat caaaaattta aactgcttgc
     4921 ctatatcaca ctcatattat tgacggtaac ttccctgaaa aacttttttg ggtttatgag
     4981 ccaaaaggtt ttagccagaa tgagggtcaa tttggtccgg aaaataagga atcaattgtt
     5041 tgaaaagttg accaatacat ccgtgggttt ttatcataat agacaaaagg ggcacttgtt
     5101 atcgaccctt agcaatgatg tgggagaaat agagaacacc gtggtcactt ccattcaggt
     5161 attgttcaga gatccttttg caatcatttt gaccttcacc gcattattta tcatttcccg
     5221 cgaactgacc ttcttcaccc ttttgttttt tccattatcg gggatagcca ttaataaaat
     5281 ttccaggggg ctaagaaaga aatctgttga gagccagagt ttgttaggta gccttatgtc
     5341 ctatactgat gaaatgattt caggtaaccg tgtgattaaa gtcttcaacg ccgaaggatt
     5401 tgtacaaaga caatacagga tgctcaatga caagtataca cgtgttgcaa agaggatcgc
     5461 caccaataag gagctggctt ctcccttgtc tgaagtattg ggggtaggtg tgattgcagt
     5521 aatcatcatt tatggaggac atctggtact tgcaggtcaa ggagaattga cggcatccat
     5581 gtttctgggg tatttggggt tatatttctc cattctgaac cccgctaaga atattggcca
     5641 ggcatatgcc aatcttcaaa gaggaatggt ctccggagcg agggtattcg aaattctgga
     5701 acagaaagat acaatccaaa ataggccggg agccgttgca atagatggat ttaaggaagg
     5761 aatccggttt gaacacgttg gtttcagata ttcgggagag acggtggtat tgaatgactt
     5821 ctgcctcaac attcccaaag ggaagacgat agcgctggtt ggtgagagcg gggccgggaa
     5881 atcaacggtg gctgatctta tcccgagatt ctatgatgtg acaaacggtc gcgtccttat
     5941 ggatggaatg gatattcggg atatcacgat ggattcattg cgcaagcaga tgtcgatggt
     6001 cacacaggaa gcgattctgt tcaacgatac agtattcaat aatatagcat tcggtatgga
     6061 tgatgtttca ttggaacgtg tcatggaagc tgcaaaggtg gccaatgccc atgccttcat
     6121 tgaacaattg gaagatggtt atcagactat tattggtgat cggggcatgc gcctcagtgg
     6181 tggccagcgg caacgcctta ctatcgcccg cgcaattctg aaggacgcac cgatcataat
     6241 catggacgaa gccacctccg ccctggatac ggaaagtgaa cgattggtgc aggatgcaat
     6301 caatcgattg acacataaca ggaccggaat cattatcgcc catcgactta gcaccatcag
     6361 tcatgccgat gagatcattg tcctgcagag aggcagtatt gctgaaaggg gaacccatca
     6421 ggaattgatg caaaagggcg gatattatag gaaactcgtg gaaatgcagc agctggaaaa
     6481 gaaataagaa gaataatcag gcattccagg ttgtcaattc cggaggtaat aaaatgacgg
     6541 cgctatattt aacccgggtt tctggcaaac aataggaagt tcattccgta agccccatta
     6601 tataaaggcc tcgttgtcct caatccgtcg ggatgatttg ggccgctgac ccgatcatcc
     6661 aatgatgcac gacattcatt tcattcttgt ccgaagaact aaaagattac tggattttgg
     6721 gggattagaa acattagtaa ataatgctaa aagttgtatc ttagcgactt caataattaa
     6781 acaataaaga ccgaaatggc gtttaatgac tttgtttcag gtctaaaaga ttatacatta
     6841 tatggatcca ttgaaggagc tttttaagac aaaagcgttt cctgtcagag atgagttgaa
     6901 aaccgtcata aaagagcacg gcgaaatgaa aattgccgaa gtgaagatca gtcagcttgt
     6961 aggagggatg aggagtatca agtcaatgtt gtgggagact tcattacttg atgctgaaga
     7021 gggaattcgt ttcagagggt attcgatacc acagctgata gaattattgc ctaagattga
     7081 aggagattct gagccgcttc cggaaggctt gttttggctt atgctaaccg gagatatacc
     7141 ctcgcgggac caggttgagt ggctaagtaa ggaatgggaa agaagatcac aggtgcctga
     7201 tgaagtgttt gccatacttg atgcctgtgg caatgacgta catccaatga ccatgttcag
     7261 tataggggta ttggcaatgc aaccgtacag catattcgca aagcgatatg atgaaggttt
     7321 tagtaaagcg gactactggg atgcaacgta tgaggacacg atgaatctga tagcccggct
     7381 gccccatatc gcagcatata tttacagaaa ggtattcagg aacaatgaac atataccatc
     7441 gaaaaaggga ctcgactggg ctgctaattt tgcgcatatg cttggatttg aaagggatga
     7501 ctttaagagc ctgatgcgac tttacctgac tattcatgcc gatcacgaag gtggaaatgc
     7561 aagcgcacat gcaacgcacc tggtaggatc aaccttgagc gatgcctatc tttcactgag
     7621 tggggggctt aatgctttgg ctgggccact gcatggtctt gccaatcagg aagtggtcaa
     7681 ctggatcaat gagatggtgg agaaaatagg aaccaagaag cctacaaagg aacagatagc
     7741 cgattatgtc aatacaacgc tagcggcagg acaggtggtg ccgggatatg gacatgccgt
     7801 attgcgcaag ccggatccga gatttatgga gcagaagaga tttgctgaaa gatatatgcc
     7861 ggatgatgat tatgtaaata tcgtatggaa agtcttcgat gttgtcccgg aaattcttcg
     7921 cggactggga aaggtgacca acccttggcc caatgtcgat gcacacagtg gctcgattct
     7981 gattcactat ggtcttacgg aaagcagttt ctataccgtg ctctttggtg tcagccgcgc
     8041 aatgggtgta cttgcacagc tgtgttggtc aagagcctta ggcttgccta tcgaaagacc
     8101 taaatccatc acttttgaca atgtaaaaga atttatcaat aatcaaaaaa gctaaaagat
     8161 cagactatga gcaacgttcc taaggaatta aagtacagta aagagcatga atggatcagg
     8221 gttgacggaa atattggcac cgtgggtatc actgattttg ctcagggaga gttaggcgat
     8281 ttggtgtatg tcgatattga caccgttgga cagaccattg caaaagatga ggtttttggc
     8341 gccgtggaag cggtcaagac cacgtcagat ctctttttgc cggttacagg aaagatcctg
     8401 gaggtcaatc ccgcaataac tgaaagcgga gacgacgatc ccagcctcat taattcctct
     8461 ccttatgaca aaggatggat tgtaaaaatt gaaatagcaa acccttccga gctggactcc
     8521 ctgctagaca gcgatgccta tgaggcattg attggttgat ggcaaaatta catattgata
     8581 gacatcgatt attcagggtg ctggcccgtg tatggatcat caccctgact attttaatgc
     8641 tattgccggg caagagtatt ccggtcgttc ggtgggcact taatattcca attgataaaa
     8701 ccattcattt tctctccttt ttttttctgg cagccggatt tttactgggc tacagatggg
     8761 aagatcgcag caaacgcagg atcttattcg ttgaattatt ggtatatgca tcaataatgg
     8821 aagtaataca acatttgctg cagcccagtc gtgcctttga aatggcagat atgctggcta
     8881 atatacttgg aataataaca gcatttattg gctattatgt tttaggtaaa tttataaaaa
     8941 aattaacact aaacccgcct aattaaaagg gtttaattaa gaaagattcc aaataaaagc
     9001 tatttttgta cctcgtttgt caaaatgaaa taactgataa ctccttttgt aaaaacagtt
     9061 gttttttgcc gccacacaac ttattgaatt gactaaactt ttattcattt taaattacat
     9121 ttcacatgaa aagatttttc ctattatttc tttcacttag ccttttttcc aaaatttcgg
     9181 cacaacagat aggctggcag cctgtttcca aggaagtggc aatgggacgc agttgggcta
     9241 gtgatgagaa tgtccccctg gaaaaccttt ttaaagtaga tttcaatgaa ctcaatcagt
     9301 ctcttttgaa tattcctgta tccgcaaaag ttggttttga tatggaaatg ccaatgccgg
     9361 atgggaaaac gcaactttat accgttgtct cagatcctgt aatggaagct gatttgcagg
     9421 caagatatcc caacataaga acctacaagg tcatctcaaa ggagaccggg ggcctggcag
     9481 gccgtatcgg cacttccccg gaaggttttt atggtgttct ggatatcaac ggtaaggaag
     9541 tactcatcaa taaagtcaac aggaacgatc ggtctttata ctctgtcttt agactggttg
     9601 atggaataaa tgctgttgat atgtttaagc caatggcctg tggtgctgat tttgctgaag
     9661 tgaagaaagt tgaagcttcc tcaatggagg ataaaattga gacaagggca gtggaagtta
     9721 agatgcagca cttcagggta ggaattgcca caacatcaag ctttgctgct gagattggac
     9781 tgaccactgt agagcaagta cttgcgaggg taacccaggt tatcaatcag gtcaatctca
     9841 gatacaacaa ggatcatgca atgcacctcg acctgattag taataccacc aacctgttta
     9901 acatgacacc cgaagacgac ttcttcaaac aacaaaacaa tggtggccag ctactgggcc
     9961 cgagtcagga attttttcag atgaaattgg cgaataagga ctatgatttc ggccagacct
    10021 ggacacagta ttgtgttgat gtgggtggtg ttgtgagcgg ttctgcctgt aataatcagt
    10081 ctaaggcacg gggggtcagt tgtggaccat ccaatgtaag ctactttctt caaacagtga
    10141 aacatgagat gggacaccaa ttcagtgcca gccacacgtt caactcctgt aatggctcca
    10201 cccaacaggc atctcaggga gcatatgagc caggcagtgg aagcactatt atggcatacc
    10261 cgggggcttg tggccctgac aatatagccg gcggagctga tgactacttt catgtgatca
    10321 gtattttgca tgtacgcggc catcatcaag catatcccga atgcggaact tttgccgaag
    10381 atgtcaatca tatgcctgaa atcaccctgc caaaatacaa ttcggatctt ctgactatac
    10441 ccaaacttac accgtacgaa cttattggaa gcgctactga tcacaatcag gataagcttt
    10501 attacatctg ggaggaattt gatcaaggaa atggtgagcc attgggaacg aattttgaat
    10561 caggcccgct caacagaacc tatccgccat taaccaataa tagtaccagg ctgattccgc
    10621 gactgaataa tatcgtaaat gatgtgtttg atatcgctga cagaatgcct gaagccacaa
    10681 gagaaatgaa ctggaaattc gtagtaaggg attataatcc aaatgcgggc gcagtggcta
    10741 ttgcagactt taaattcagg gttgattcaa acgcaggacc cttcaaattc agcttcccca
    10801 acaaaaacac cgattctgtc cttactgtag gtcaatactt cgaagtgaag tgggatgttg
    10861 ccaatacgga taaagaacct gtgaacacca aatttgtgga cattttttat tctactgatg
    10921 ggggtgtgaa ctacaaagat actttagtta taggtacacc caatgatggt gcagaatatg
    10981 taacaattcc ccaaaaagct cagaacagtc gatttaagat taaggggcac ggcagtatct
    11041 tcctcgatat gtccagaaag accttgcgtg tcgtggagcc ggatagcgcg gggttctcct
    11101 atgatgtata tccccataat gcactattat gcccgttcga accgcaaaac ttccttataa
    11161 aaggtatttc ctggaaagga tttgataaac cgataaaagt ggaattggtt gacggatggc
    11221 cttccaacac ggttattaag ctagacaagg agattatgaa tccgggagaa acgctgacgc
    11281 tttcaatcga caccagagat gtgacggatg ccggtttcta taccatcaga atgatagcta
    11341 cagcagaggg tcaggatacg atatggcgat atatggaagt ggaaatttta ccttcaaaac
    11401 tggaatttac cggggctgtc aatcctttgc cgggtaacac gaatgagtca caggttccgt
    11461 ccttcgcctg gaatattata ccgggtgcag atcgatatga gttccagttg gcttccgatt
    11521 ccaaatttga aaacatacta catacctaca ccggcacggc tattgcaata gagcctcagg
    11581 tggtacttcc tcctggcgca atctattact ggagggtaag ggcaagtaac agatgttatg
    11641 atggcccatg gtcggaatac aatacctttc agaccatact gtttgaatgc aatacaatca
    11701 atgctctggg ccttccaaag gcgatatcga gcaatgtcac agctccgccg gtggatgttc
    11761 tctttgacat aagcaacagc acaggtgtgg ttgccgatat caatgtcaaa aatattgata
    11821 tcagccactc cgatatttca aaattgaaga tttccgcaag gtcaccaagt ggcaaggaaa
    11881 ttaacttaat gacaggcaga tgtgcaggta tttcagggct ttacacaact tttgacgatg
    11941 tgtcaccact ggaaatcaat tgcggtttga tcaaagcaaa taagacattc aaacccgaag
    12001 aggaactttc agcgtttaat ggcgagccgg tacaaggcaa ttggtccatt cgagtcacaa
    12061 ctatcagagg gggatcctcc ggaaagatca acaatattga ttttgacctt tgtgcaagtg
    12121 ttaccacacc gcctatcatc aaagtcaaca ataatttgtt caaggttaat tctgaccata
    12181 gtcaggttct ggaaaataaa gatttgctgt acacctgtga aaataccggt gatggaaata
    12241 tctggtacat catgctatca gaaccggtac atggatattt cacactatac ggacaggaaa
    12301 ttcaaaaggg tgatcggttt actcaaggcc acctgaacga ctggggggta caatacgtac
    12361 cctatgattc cgaatatgaa ggaacagaca aggtcgactt cctggtgcgt aatgataagt
    12421 ttgcttattt ggcaccggaa caatttaata tagacctgag taaaacacat actgtatcaa
    12481 caaagccgat caataaggat atttctaaat tgatcaaatt ctatccgaat cctacaaatg
    12541 gtgaggtgaa tattgacctt tcagacatca aaatcctgga aacaggtaag ttgacagtaa
    12601 ctgatattgc aggtaaagtt gtaatgcaga aaccgattcc tgccaatacg gatagggttg
    12661 tacttgatct tggcaggatg tctgcaggag tctattttgc cggcatttat tcaggaaatt
    12721 accatgcaat gcaaaaaata atcaagcagt aaattttatt caaaccagta tgtcatcaga
    12781 ggctttttcg gattccttgg aaggagggcg ttttcgggaa agcctctgtt tttttctgaa
    12841 tcgctccatt tttaattaaa aaccgaacat agtatgaaga tcatcagact tcttgcattt
    12901 aacttactat tcatttcttt atatacgaca agccgtgcac aaatcaatat aaactggcag
    12961 acagcaaacc catcctcaga acaaaaagca gagatttcgt ccatgttgct tttgccacag
    13021 gatgccaggt tttacaaagc ctccttcctt cataccacgc tggagagtgt ttccggatca
    13081 gaagttgtac aagtaacctt accaattggt atggatggtg ctgaagtaaa agttcacttc
    13141 gtttacgacc ctgtcatggg agcaagggat cagcaaaaat tcaaggagat taggacattt
    13201 aaagcatttt cgaccgaggg cacaccaata tcaggaagag ccggtattag tccgacaggg
    13261 ttctatggag tattcgaatt gaatggaaaa cagatcatga tcaggccggg tgctgttgaa
    13321 caagatatgt atgttgtata tgatttgggg cgtaaattgt ccatgctcga ctttagtctt
    13381 cttgccggtt gtggaacaga aacccacctg aatgagatta aaaggaacga gactgtcaat
    13441 ttgcggagtg atgacaaaag aatgcgcaac tttaccattg caatcagctg tacttcggga
    13501 tatgccgatc aggtgggtcg gtcggagggt caggttatgg ccagtgtcgt tgaagtactt
    13561 aatcttctca actatcggta caacgtagat tttggcatta agctcaattt gcttgacgga
    13621 acatcccaat tgtttaatct ggatccccat accgactatt ttgtcaatga gacgaagggg
    13681 ttggaactgt tgcagcagaa tcaggatttc ctggatagcc tgattgataa ttcatcgtat
    13741 gatctggcac aagtgtttac acgagggtgc agcgatgtgg gcggtgtcgt gtgggggcgt
    13801 acctgtgtca gtgataccaa ggcacgtggt gtatcctgta ggaccaatga tagcgactat
    13861 ttctttacga cttttaagca tgaagtggga catcaattca gtggcggtca caccttcaat
    13921 gcttgcaatg gaagcaccca gtataacccg ttttcttctt tcgaaccggg agccgggagc
    13981 acgatactgt catatggaga caattgtgga tcagataatg tcggcgaaag ggtggattat
    14041 tttcatgtag caaatattat tgaagtcaat cgttatgtca aggaaataga gaacaaatgc
    14101 ggaacatggg gagcggagat caatcataca cccgtaggct acctttcaat gcccgaagac
    14161 aaaacaattc caatccttac tccattcgaa ctggagggag gggcgacaga tgatgataag
    14221 aatcttacat tcaactggga gcaatacgat ctgggcgaag gagagcccct gggcaccaat
    14281 tttgaaacag gacccttgtt tgtgtctcag gctcctggcg gtgtagggca cagattattc
    14341 ccttccttgt ccacaattaa ttccggaatt atttccaatc gggagcgctt gccggaggta
    14401 tcaagggaga tgaatttcaa aatgaccgtg cgcgacaata atccaaatgt tggggcccaa
    14461 gctatcgtgt cctataagtt cgatgtaacg gatcaggcag gaccctttaa ggtgacattt
    14521 ccggatgccc agacagatac tagttttact ttgggacagt atgttgagat tacctgggat
    14581 gtcgctaata cagatcaatt tccggtcagt tgcaaaaatg taaatattct ctttagcact
    14641 accaatggca atacatggtc tgatacattg gttgccaata ccccgaatga cggcagagaa
    14701 tcggtaatga tgcccaggac ttcaggacgg gcaagattta aggtaaaggc agctgacaac
    14761 atctttctgg acctttccgg aaaacccttg aaaatagtgc aaccttcaga agcgggttat
    14821 agtctggata tcctgcccca tgatatatcc ttgtgcaaaa acagtattta cacgcttact
    14881 gtaaagtctg tcagttggaa agcttttaat tctcctgtaa agatttcggt gtttgattca
    14941 ggacacccag cttttaaggt cattcctcta aaaagtgaat ttctgccggg agagacaatg
    15001 accctggacg tcgatgtgcc ggatgtcata gatgccggaa cctatcagat ccggtttatg
    15061 gctacttcag atcaaggcga tacacttttc agaacagtta cattgaccat tgcggacaac
    15121 gatcaaaaca tttccggaac catcagtccg gttgccaacg atcagtacgt aacgcttcat
    15181 ccggtttttg cctgggatcc cgtggaaaat acccatggct atcaattaca attggcgacg
    15241 gatccggcct tttcatctct gatcctgaat gtgtcaatag actcagagga gtatttatcc
    15301 gaaatagaac tcaataaagc gaccatctac tattggcggg tccgctcttt gggggaatgc
    15361 ggttatggtc gctggacggc aatgagtatt ttcaggacag agtccgaatt gtcaaacagc
    15421 gcactacatc agatcaataa taagatctta aaagtcaaga ctaatgagaa aaagccggcc
    15481 ttacatgaag atttgtacta cgtatcagaa ttgaatcaga acgaatcact gaaatatacc
    15541 ctgctttcag agccggagca cggcgccttg tatctggata acgcacgctt aaaaaaagga
    15601 gatacattta ctcaggaaaa tgtcgatgcc ggaaaatttg ttttctctgc agaggaattg
    15661 gattatcaag gaggagatgg atttgccttt ctggtgtctg atatttccaa tctcttcata
    15721 ggaccggaga attttataat cgatatccgg gaaagttatc catccggaac atctcatatc
    15781 aatatggatg ccatggttga tctggtgccc aatcctactt ccggactgat agatttgaaa
    15841 tgtgttggaa tggacctgaa ggaggatgtg gagattcaga tcttagacat ccgtggagta
    15901 tcagtgatga gaccgtttaa ggcccaatta aatttgggga accgcataga cctgacttct
    15961 ttgtcttcag gcgtctattt gattgtggta caatcgggaa attatattgc caaaaaaagg
    16021 ctcgtcaaaa tctaatcaac tatatttgcc gggtaaaatc cacattgact gacttacgtt
    16081 agcgttataa tgtggtaaca tcgttgtatt atgttccggt cgaagtattt ctggactcat
    16141 gttggtttga tgattctggc tgttggtctg ctgacagggc tgattttttt ctttttaggt
    16201 ctatataccc accatggaga gaaaatcccg atatccggac taaatgggct tcaggtcgag
    16261 ttggcaaaac ggcaagctga agaagatgga tttgaggtag ttgtcaccga ttcggttttt
    16321 atcgtcggac aaaagggcgg tattgttctt gcacaaaatc cagaaaaggg aacctttgcc
    16381 aaggagggta gaaagattta tgtcacgcta tccaagtata tgccggacat ggtgcccatc
    16441 ggttcactcc ctgcattgta cggaaagaat ttcgaattga aaagaaaggt tttgatggag
    16501 gggtacgaga tacaatcggc agttgtaggc caattgtatg atgccgggga gccgggctgt
    16561 atcctccggg tgatatacaa taatgataca atcattgatg cgaggggagt acgggacaat
    16621 gtcaatcttc ccaaaggcgc caaattgaag tttatcgtat ccaccaacca gggagggcag
    16681 atggaggtgc cggatcttat ctgtcaagag tttgatgagg caaggttttc tttaggaaat
    16741 aattttgaat tgtccggacg ttccggcaca tcaggcaaat atatagtggc acaggaaccg
    16801 gcatatactc caggtgtgaa gatcgacaga gggacaacaa tcatcgtcga tctgagtgat
    16861 tcacgtcctc ccggatgccc gcctgatgaa gatgaatagc aacaaataag acagacattt
    16921 cgtttttaaa taaaaaatat cgaatgaatc gtttttcact gaggattttc tttttcttat
    16981 ggatattgct gaccggtgta agtagcggcg ctcaggtcgt ctattatgga ttgaccgaaa
    17041 accctgttct gttcccagaa aagcaagtat ctggacagag aaatttaatc agtgacagta
    17101 gagtggttgt cataccctcc gatagtcaga cactctgcat caatcgtccg gagataggaa
    17161 cctttgatac actgatcctt gatcaagcct ccgtcaatcc tggtattgat gtgaaaataa
    17221 cggagaattg cattgtattt attgcagaac aaaacgcaga attaggggat tatgaataca
    17281 cctttgacta ttcgtccttt tccggcaact ccctgaaata tagggttgca ttgtcggttg
    17341 tggagccgag aggattgcca ttcttcgatg attttgccta caacgacaag tatcctgatc
    17401 aacgaagatg ggtcgacaaa aatgtattca tcaacaatac aatgggcgac aatcctccaa
    17461 gtgtgggtgt tgcaacattt gacgggttga acagcaatgg aagcccttat ggtggtggat
    17521 tcggtcgggc cgactacctc acttccacat tttttgatct aagtggagta caacattctg
    17581 aaccggtttt tctgagctat tatctgcaac ccaaaggaaa gacgtataat catcagctgc
    17641 gtgatagcat tgagct
//
LOCUS       DAOQMC010000014        17567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1140_length_17567_cov_4.752855, whole genome shotgun sequence.
ACCESSION   DAOQMC010000014 DAOQMC010000000
VERSION     DAOQMC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17567
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_17567_cov_4.752855"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..2716)
                     /locus_tag="PKV31_01165"
     CDS             complement(251..2716)
                     /locus_tag="PKV31_01165"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNB91487.1"
                     /translation="MLDLTARKICEIVEGTLYGLSERQLIRRISFDTRQIFHAHEVMF
                     IALPGEHRDGHNYIKKAWDAGVRCFLVSRKVDFESFGDSVFILTENTLLGLQAIAAYH
                     RSLFKIPVIGITGSNGKTIVKEWLYQMLSGEINIAKSPKSYNSQLGVPMSVITLRPEN
                     ELAIFEAGISQKGEMDRLEAIIQPTIGILTYMGAAHSEGFESKTAKIEEKLKLFKHAD
                     FVIYQKKDNTHEAIQRVLPETRKFSWGVNVDADVIFIIEKVKTRKTIASVKYLGNIYK
                     IKIPFTDDIYLHNAFTCITYLIHAGYSIELIESMIGKLQPLPMRMELKTGIFNSVLIN
                     DTYSSDINSIALAFAAARKEAGKGRMAFIVSDFAYSDNHTHDDYEVLVSLVNEFRPAL
                     FIGVGEKVRNIRIKMDPEIRASFFHNTESLLQGMDITQFRDTTVLLKGARRFGFEQIV
                     DRLEQRAHEAALEINLSALEHNLNRYRAKITYPVKLMVMIKASAYGSGSVEVARVLQQ
                     QGIDYLAVAYIDEGIYLRKAGISLPIMVMNSGMDFVERMVEYDLEPEIYAPAQLDVLI
                     QYLFSADERLKCHIKLDTGMHRLGFTKDEIPFLIEKLKGNSHLEVVSVFSHLAGSGES
                     LFDEYTHFQAAYFEEMVSSISRGLRTHPLRHILNSSGIARFPQYMYEMVRLGIGLYGF
                     DPSGFVSDLHTVMTLKARISRIKTIPTGSTVGYDRRWKAEKDSIIATISIGYADGLRR
                     SAGNGRWKVLIHGQPATIIGGVCMDMAMVDATHIEGVREGDEVVIFGPTNRADELAKV
                     YETIPYEVFTGISSRVKRTYILE"
     gene            3541..3933
                     /locus_tag="PKV31_01170"
     CDS             3541..3933
                     /locus_tag="PKV31_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91488.1"
                     /translation="MARKATVTLNVIDSLAKKIKSLLNNTLQKVSFHNLRKEMYLSSS
                     YLFILFIAFEKSFINYEGKDSGRTKAPSFSSKATQFGAVRLRELENKYNQNNLLLLLQ
                     TTRVEIIADKSGSHHITRIVTRILKKIN"
     gene            4048..4485
                     /locus_tag="PKV31_01175"
     CDS             4048..4485
                     /locus_tag="PKV31_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91489.1"
                     /translation="MLRLRCVEPEDEENTRTELPEKLSTFEELEVNGEAESFQKAFDV
                     ITGKRPDSAFMDIKLIGGSIFILLEKLISHGIPIPNIFIATGFSENLLTTDNAFHNYI
                     VRHLIKPYMDDWEVKLSKAIDALQTTRIRQLITTEGNKLPESV"
     gene            4744..5736
                     /locus_tag="PKV31_01180"
     CDS             4744..5736
                     /locus_tag="PKV31_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HNB91490.1"
                     /translation="MNTGSNSIRKLLRYSCVIVFLLAACHAAFSQTRFIPFAFGDMDK
                     WINRDVKESFVIGGKTQTLYEIADTRDGVSPNHAYDNKFSPWATSSVLATINGITKGS
                     VTVFPEKRGDGFAARLETRVERVKVLGILNINVLASGTIFLGKVAEPITDTDNPFSKL
                     IMGIPFTGKPAWLQFDYKVTAGGSSRKINGLSSNGTATGKADNAIVHVYLQKRWEDKD
                     GNVYALRIGTGGEIFSQTINDWQKSHRVKIHYGNVSNYGNLPAIFTRLKKGNDAFYTR
                     NSKGKMVPIHEEGWGSPNNEVTHIILQFSSSDGGPYVGSPQNKLWVDNVGTVYD"
     gene            6225..7886
                     /locus_tag="PKV31_01185"
     CDS             6225..7886
                     /locus_tag="PKV31_01185"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB91491.1"
                     /translation="MTKIEEMAIRLLTLLTIGGLFTSCGSKKSIDLLVYNATIYTVDS
                     TFSIAEAFAVDKGKIVETGRSEDLKSKYNARESLDAGGKYVYPGLIDAHAHFIGYGQS
                     LNRVSLVGTTSWEDCVNRTREFAKKNDVEWIQGRGWDQNDWAEKEFPDNQALNELFPD
                     IPVFLTRIDGHAAIANDKALQITGIKAGDKIDGGIIEVKDGKLTGVLVDNAVDLVSSK
                     IPPMNVSELTEALLEAQLNCFAVGLTTIDDCGLDHEVIDVIDSLQKSGQLKMRLYVML
                     SDVRKNYKYLIDKGKIKTDKLNVRGFKIYSDGALGSRGACLLHPYSDKPGWTGFLLSS
                     KEHFDSVANVIYTHGFQMCTHAIGDSANRVLLETYAKYLKGPNDLRWRIEHAQVIDAN
                     DFKFFSDYNIVPSVQPTHATSDMYWAGERLGKERLINAYAYHHLMEQNGWIPLGTDFP
                     VEDISPFKTFYAAVVRKDAKGYPEGGFQPENALTRKEALRGMTIWAAKSNFEENEKGS
                     LEIGKWADFIITDRDLMTTEESDILGTKVLKTFIAGEKVFEADKK"
     gene            8026..8484
                     /locus_tag="PKV31_01190"
     CDS             8026..8484
                     /locus_tag="PKV31_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNB91492.1"
                     /translation="MKKLILPSLIIALFLVACNFNNKTPSNDTKSVKLPPDPSSIVYN
                     TDWILKDMNGKEVKISPDAPKKAMIHFDKEDHVTGSGGCNLFSGNCKFIDNEKIAISY
                     GAATQMACPEMDIESEFFATLGKAEKYKLDGNKFYLMDKNGTVLLSFTKD"
     gene            8636..10318
                     /locus_tag="PKV31_01195"
     CDS             8636..10318
                     /locus_tag="PKV31_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB91493.1"
                     /translation="MKGENKKYNTDKILAMKKCFFLFLFIGLGTWSVAQRNIITDQII
                     VKIKDCYLQNSRDKSNLAPSITQSFTLLGERFKALDTKNLYLGKNHSMAYFTILFPPG
                     SDIDEIITEYKKLEEIEYAEPDYIGYGAGGDGFIPDDKFYVKQWGLNNNGTFQHAQGI
                     AGIDVNMEESWSITTGDSNIIVAILDSGGKLDHPEFAGRIWQNHKEISGNNVDDDENG
                     FTDDFQGWDFVNKDNNPSDDHGHGTNVGGIVGSTGNNGRGFAGVDWNCKLMFLKVLNS
                     NNTGYQSSFIAAIYYAVDHGARVINMSIGGGQSDIYRDAVNYALQKNVTIVACMMNHN
                     TEAPYYPAAFPGVIAVGSIDPNGMRTQPFFWNSSSGSNFGSHISVVAPGNYIYGLSHS
                     SNSNYDTYWGGTSQATPHVAGVVSLMLAINPNLTPKEIKNILETTARDGIGRPEEDTP
                     GWDKYHGWGLIDSYKALTYSVSSRKNPVHFPDFSVFPNPARSALQLIFQTPVTEGEVY
                     ILDLLGRQVYSNQKINGDKLEIDCSEIPTGIYTIVLQNKGSTHTGGKLLIIH"
     gene            complement(10527..13298)
                     /locus_tag="PKV31_01200"
     CDS             complement(10527..13298)
                     /locus_tag="PKV31_01200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB91494.1"
                     /translation="MNLLFRINRLKTAFFVGLALMILPSLIQAQVEGFVKGPSVEGIT
                     EYTLTKNGLKVLLFPEPSKPTITVNITYLVGSRHEGYGETGMAHLLEHMVFKGTPNHP
                     DIPSELTKHGARPNGTTWLDRTNYFETFAATDENLEWAIDLEADRMVNSFIAKKDLES
                     EMTVVRNEFESGENNPISILMERVISTAYLWHNYGKSTIGARADIENVPIERLQAFYK
                     KYYQPDNAVLVVAGKIDVNKTLELIKKHFGKISNPDRTLAPLYPTYTLDPVQDGERSV
                     VLRRVGDVQVVMAAYHVSPGAHPDFVALDLLASILTDNPSGILYKELVENNKATSVDG
                     FGFELKEPGIMMFGATVPLDKSLEEAKMILLGRLDKVKGMTFTQEELDRQKQKMLKSW
                     DMTFNNVERIGTGLSEYIAQGDWRLMFINRDNIKKVTLEDLQAVATKYFKNSNRTIGE
                     FIPDKNPDRVEVPGPIDVAAVVKDYKGNAAVASGEAFDPSPNNIDERTTVAKSKSGNF
                     EVAMLPKQTRGNVVIANLTIRFGELNSLKNKSSIGDLTAAMLDKGTKTRTKQQITDEF
                     DKLKSRVSFSGGATSCNVRIETTKENLKPTLAIVADILKNPSFPQNEFDIMVTQEVSG
                     LEEQMSDPQGLAGIEFARAMSSYPKDDPRYTPTLSENIEMLKAVKLGDLKKFHSDFYG
                     ASKATASVVGDFSKAEIEKMLLETFGNWKSKMPYKRIASPYQPTKAGEKTINTPDKSN
                     ALYLAGYSIEISDTDPDYPAMVLANYILGGGFLNSRLATRIRQKEGLSYGVGSGFSAN
                     ALDKTGRFMVYAISAPENSERVNICVKEEITKANKEGFTAEEIEAAKSGLLQSRQVSR
                     AQDASLAGTLNSNLFLGRTMQFDGKIDDAIKALTPDQVNKAFNKFISADKLVVVRAGD
                     FEKDVEKKP"
     gene            14149..15117
                     /locus_tag="PKV31_01205"
     CDS             14149..15117
                     /locus_tag="PKV31_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HNB91495.1"
                     /translation="MLAIVYDNPGPTSVLHPKNRPVPAVGENDVLIRTVAAGVNRPDI
                     IQRLGKYPAPAGAVQDVLGLEVSGFIERIGRNVTRWRRGDEVCALIPGGGYAEYVSAH
                     EGSCLPIPAHISLEDAAALPETLFTVWHNVFQRGGLKKGETALIYGASGGIGSMAVQL
                     ISIYGAHAFALAGSPEKAAFCTSLGAEKVIDYTQNNLLDTLLSESIDLILDSLGGKFL
                     DIGLTLLKPEGRLVYINAMEGNQPGLDIMKVMKKRLFITGSTLRPRGLDFKASLANDI
                     KQYAFPLLEDQRFKSVVNYRFPAKNASDAHELMESRDFTGKIILQF"
     gene            15184..16623
                     /locus_tag="PKV31_01210"
     CDS             15184..16623
                     /locus_tag="PKV31_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNB91496.1"
                     /translation="MSITHLEPSALWRNFSALNAVPRPSKKEGRVIAFIKKFGESLNL
                     ETRVDETGNVIILKPATPGMEGRKKVILQSHLDMVCQKNNDTEFDFDTQGIRMVTDSD
                     WVMAEGTTLGADNGIGVAAIMSILESKEIDHPELEALFTIDEETGMTGAFGLKPGALS
                     GDILLNLDTEEDDEIDIGCAGGMDVTATGQFITESYSGIGIQIEIKGLRGGHSGIDIH
                     EGRGNANVILTRFLFLGLNDDIRLVNINGGGLRNAIPREAKAVVVFTQGSHFLQQAEQ
                     LKVEILHEYLTLEPTLEINISPSEVNAAIMNTADSGSFIRSLKVAHNGVFRMSPTVPG
                     LVEASNNIARVTLDDGKITVMNLTRSSVDSTKQAVAEQLRAAFELSRMEVTYSGSYPG
                     WQPNPHSSIVELMQRIYVNKFGSAPKVVACHAGLECGIIGAHYPKMEMVSFGPTIQGA
                     HSPEERVNIKSVGKFWSYLLDILKEIPEK"
     gene            16794..17219
                     /locus_tag="PKV31_01215"
     CDS             16794..17219
                     /locus_tag="PKV31_01215"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNB91497.1"
                     /translation="MIVAKTFKWEAAHRLPRHEGKCKHIHGHSYKMTVEFEGEPNANG
                     MVIDFNDIKKIIQPHIDNIDHSTMIAEGDDELKEVFDSKSWRYYLLPFDSTAENLCRF
                     FLDKIILENRDLLAQYKITAIGIKVFETGTSFAYARATL"
BASE COUNT         5345 a         3700 c         3450 g         5072 t
ORIGIN      
        1 ctgctaccgc tacaacaggc accgcatgaa aggtgaaatc ctggatgaca tcatctccag
       61 aatggtcagg cataggccga tgaaagtaaa aaaggtaaaa tctatctgaa ttgattaatt
      121 gctgaacctc taccaattcg attctaactt cactgagcaa ttaccttcta attgaaatct
      181 tacaagggaa atgcttcacg atacaagtac gtactgattt tcagcagaat aaaagtaatg
      241 ataattataa tcattccaaa atataagttc tttttactct tgaacttatt cctgtaaata
      301 cctcataagg tattgtttca tacacttttg ccaactcatc agctcggtta gtagggccaa
      361 aaattacgac ttcgtcaccc tctcttaccc cttctatatg agttgcatca accatagcca
      421 tatccataca cacccctcca ataatggtgg cgggttgccc atgaataagc accttccatc
      481 tgccattacc cgctgaacga cgcaaaccat cggcataccc aattgaaata gttgcgatga
      541 ttgaatcctt ctctgccttc caacgtcgat cgtacccaac ggtactgcct gtcggtattg
      601 tctttatacg cgatattctc gccttgaggg tcataacggt atgcaggtct gaaacaaagc
      661 cacttggatc aaatccgtat aacccaatcc ccaggcgaac catttcatac atgtattgtg
      721 gaaaacgagc gattccactg gaatttaaaa tatgtcgcaa aggatgggtt ctcaaacccc
      781 tggaaataga actgaccatt tcttcaaaat atgctgcctg aaagtgcgtg tattcatcaa
      841 aaaggctttc tccactaccg gcaagatgag aaaatacgct aaccacctcc aaatgtgaat
      901 tgcccttcaa cttctctatt aaaaaaggta tttcatcttt tgtaaatcca agccgatgca
      961 taccggtatc cagtttaata tgacacttta atcgttcatc agcactaaaa aggtattgaa
     1021 tcaatacatc caattgtgca ggtgcatata tttcaggctc cagatcatat tcaaccattc
     1081 gctcgacaaa atccatccca ctattcatta ccatgattgg caaggagatt ccggctttcc
     1141 tcaaatatat tccttcgtca atataggcta ccgcaagata atcaatgcct tgttgctgaa
     1201 gaacacgggc cacctccaca gaacccgaac catatgcact tgccttaatc atcaccatga
     1261 gtttgacagg gtaggttatt ttagctcggt agcgatttag attgtgctcc aaagccgaaa
     1321 gattgatttc caaagcagct tcatgagccc tttgttcaag cctgtcaaca atttgttcaa
     1381 aaccgaaacg acgcgcacct ttcagtaaca cggtagtgtc ccgaaactgt gtgatatcca
     1441 taccttgcaa caaactttca gtgttgtgaa aaaatgaagc gcgtatctca ggatccattt
     1501 tgatcctgat attcctaacc ttttctccaa ccccgataaa cagtgccggg cgaaattcat
     1561 tcacaagact caccaacact tcataatcgt catgtgtatg attgtccgaa tatgcaaaat
     1621 cagagacaat aaaagccatc cttcctttgc cggcttcttt tctggctgca gcaaatgcca
     1681 atgctatgga attgatgtct gaactatagg tatcattgat tagtaccgaa ttaaatatac
     1741 ctgtcttaag ctccattctc atcggtagtg gctgcaattt acctatcatg gattcaatca
     1801 actcaatact atatccggca tgaatcagat atgtaataca tgtaaaggca ttatgtaagt
     1861 atatatcatc agtaaatgga atcttaatct tataaatatt gcccaaatat tttacagatg
     1921 caattgtctt gcgtgtctta actttttcta tgatgaaaat aacatccgca tctacattta
     1981 ccccccaact gaatttcctc gtttccggaa gtactctttg aatagcttca tgtgtgttat
     2041 cttttttttg ataaataaca aaatcggcat gtttgaaaag ttttaatttc tcttctatct
     2101 tggccgtctt cgactcaaaa ccctcactgt gagctgctcc catataagtc agaataccaa
     2161 ttgttggttg aatgatggct tccagcctat ccatctctcc tttttgagaa atacctgctt
     2221 caaaaatggc aagttcgttt tccggacgga gcgtgatgac tgacataggc actccgagtt
     2281 ggctattata acttttcggg ctttttgcaa tgttaatctc accggataac atctgataaa
     2341 gccattcctt gacgatggtt tttccattac ttcctgtgat gcctataaca gggattttga
     2401 aaagactcct gtgataagcg gcaattgcct gcaatcccaa caaggtattt tcggtcaaaa
     2461 taaaaaccga gtcaccgaat gattcgaaat caacttttct gctcactaaa aaacatcgaa
     2521 cgccggcgtc ccatgctttt tttatgtaat tatgcccgtc acgatgttcg cctggtaagg
     2581 cgataaacat tacttcatgg gcatgaaaaa tttgccttgt atcaaatgat atccgacgaa
     2641 ttaactgtct ttcagacaaa ccatagagtg tcccctctac aatttcgcaa attttcctgg
     2701 cggttagatc tagcatattt gcaacaatac aaagttatta ctaaactata tatttttgat
     2761 atatttataa gcattgaatc caaatttagt attggtagtt ataattcgtc tactatattc
     2821 ttccaattac tgatctctat gtattatcct tttcaatctt cacatgattc attatctttt
     2881 ctttcatttt ttctgtctga ttaagaaagt ttgctcggat aattagtcac tatttcaagc
     2941 tattaccttt cagagtgaaa ataaaacctt gcatactact gagcgatgaa accatttaga
     3001 acccattctt gagtgaggaa gaaaagactg aataatttca tttgatatag attttataaa
     3061 ttttgaaaga ataattatcc tgtaatgtga accatacatt atgttccatt tcaataccat
     3121 ctcagcgagt ttactctatt acaatctgtc aatataatat cctctgaatt atggacctgg
     3181 atgccttaga tgtatgaaga tcacgtgatg tacctaaact tgaattgcag aagatattat
     3241 gtaagacaat gttcaaagaa gaatgggatt attttgtagg caaaaaggct ttcgttcgtc
     3301 atttcacccc ttttgtgggt caaattacac ggttggcggg tcattctttt tactactgaa
     3361 tatgtaagcc tatttttgtg tgacaagtaa ataaaaattt ataagcataa attcatgata
     3421 tcccatcaaa agacaaattt tccaatgatg tatagggata tggacgtcca tagggtttat
     3481 gcacctttca attggcccaa tccttatttc atccacaata ccttgctaaa gcttccggtc
     3541 atggcaagaa aagccactgt gactttaaat gtgattgaca gccttgctaa aaaaatcaaa
     3601 tccctattga acaataccct tcaaaaagtt agttttcaca acctaaggaa agagatgtat
     3661 ctatcctcca gttatttatt cattctgttt atcgcgtttg agaaaagctt catcaattac
     3721 gaaggcaaag attctggacg cactaaagca ccttctttta gttcaaaagc aactcaattt
     3781 ggagcagtca ggctcagaga acttgaaaat aaatacaatc aaaacaatct acttctttta
     3841 ttgcaaacaa ctcgtgttga aattattgca gataaatcag gaagtcacca tataacgagg
     3901 attgtgacga gaattctaaa aaaaataaat taataattac ctgaaaaaaa ataacgccta
     3961 tggataattt ttaaagaccc ttagtataac cctatagtaa aaaattgtca acatatctat
     4021 aaatccaaaa tttagtttac gactaacttg ttgagattaa gatgtgtaga accggaagat
     4081 gaagaaaata cgcggactga gttgccggag aagttgtcca cgtttgaaga gctggaagta
     4141 aacggcgaag cggaaagttt ccagaaggcc tttgatgtca taacaggaaa aaggcccgat
     4201 tcggcattta tggatatcaa attgatcgga ggtagcattt tcatacttct tgaaaaacta
     4261 atatcccatg gcatcccaat tcccaatatt tttattgcca caggattttc agaaaaccta
     4321 ttgactacgg acaatgcatt tcacaactac attgtccggc acctcatcaa accttacatg
     4381 gatgattggg aggtcaaact tagcaaggcc attgatgccc tgcaaactac ccgaatcaga
     4441 caactcataa cgacagaggg gaacaaactt ccggaatcag tttaatattc atccatacaa
     4501 tccgggaaga acctaatcag aaaacaatat cttgatgtat gatatctaaa aacgatgtaa
     4561 aaggcaagat cacaatcata ttctatcata acatttgcaa taacaattaa tccaggcaat
     4621 aagatttaga atgaggtaaa tttttatctg tcctctttta tcataatctc ggttatttca
     4681 tcgttacatt ttaaaatcat catacaattg ctcaaaaaat tattcaattt caaagataga
     4741 ctcttgaata caggttctaa ttccattcgg aagctgctta gatatagctg tgtcatagta
     4801 tttttattag cagcctgcca cgcagccttt agtcagacca ggttcatccc ctttgcattt
     4861 ggggatatgg acaaatggat taaccgtgat gtaaaggaat cattcgtcat tggtggtaag
     4921 acacagacac tttacgaaat cgctgacact cgagacggtg tctcacctaa tcatgcctac
     4981 gacaacaagt tttctccatg ggcaacctct tctgtacttg ctaccatcaa cggcatcacc
     5041 aagggcagtg tgactgtctt tccggaaaag cggggtgatg gctttgcggc acgtctggag
     5101 acacgcgtag aacgtgttaa ggtgctaggt atactcaaca tcaatgttct tgcctccggc
     5161 accatatttc tgggcaaggt tgcagaaccc ataactgata cagacaatcc attcagtaag
     5221 cttattatgg gtatcccatt tacgggaaag cccgcttggc tacaatttga ctataaagta
     5281 acggccggag gatcatcacg taagatcaat ggcttaagca gcaatggtac tgctaccggc
     5341 aaggccgaca atgcaatagt acatgtctat ctccagaaac gatgggagga taaggatggc
     5401 aatgtatatg ctctccggat aggcactggc ggcgagattt tctctcagac aatcaatgac
     5461 tggcagaaaa gtcaccgtgt caagatacat tacggcaatg tctccaacta tggcaatctg
     5521 cctgccattt ttacccgtct caaaaaggga aatgacgcct tctatacccg aaacagtaaa
     5581 gggaagatgg ttcccatcca tgaagaaggc tggggtagcc ccaacaatga agtcactcac
     5641 atcatcttgc agttttcatc aagtgatggc ggaccctatg tcggtagccc tcaaaacaag
     5701 ctatgggtgg acaatgtcgg aacagtgtat gattgaaatg tatgttggaa atttatttcc
     5761 atattacata ttttgagatg ggagaccgga gatttgattt tgaattacca tgtatcctga
     5821 ccccgtacag caattgaata acctttgggt tccgggtgaa ttttgcccaa atatgtaatc
     5881 aatttttttg caggaactcc aattaatcgt atcagctttc tacccaataa tgatctatat
     5941 gtactcttcc ttcatttgaa atcatcatta ttctatatag atctatccat gatatacaat
     6001 gtttgtcggt cgtcacactt acttttgtat tacaaatagt atcattataa tacacgagta
     6061 ttagccagga acaaactctc tgtgttgctg ccacatcatg aattaaggct agttttgatt
     6121 ttttggcagt ctctttcgat tctttggtta aggctgatta catttgtagc accaacgaaa
     6181 atgaaaaagt caaggtaatg tattctcttc aaaaactaat ttgaatgact aaaattgagg
     6241 aaatggctat tagattgttg actcttttga ccattggcgg tctgtttacg tcatgtggaa
     6301 gtaaaaagtc catcgacctg ctagtctata atgcgacaat atatacagtt gacagcacat
     6361 tcagcattgc agaggcattt gctgtggaca agggcaaaat agtggaaacc ggcaggtcag
     6421 aagatctgaa aagcaaatac aatgcacggg agagtttgga tgcaggcgga aaatatgtct
     6481 atcccggcct gatcgatgca catgcccatt tcataggcta cggacaaagc cttaataggg
     6541 ttagcctggt gggtacaaca agttgggagg actgtgtcaa cagaaccagg gaattcgcca
     6601 aaaaaaatga cgtggaatgg atccaggggc gtggctggga ccagaatgat tgggcggaaa
     6661 aggaatttcc cgataaccag gcactaaacg aactttttcc agacataccg gtttttctga
     6721 ccaggataga cggccatgcg gccatagcca atgataaggc attacaaatc acaggcatta
     6781 aagcaggcga taaaatcgat ggcggcatca tcgaagtaaa ggacggaaaa ttgaccggtg
     6841 tcctggtgga caatgcagtt gatttggtca gctccaaaat tccacctatg aatgtatccg
     6901 aattgactga ggcattactt gaagcacagt taaattgttt tgctgtaggc ttgactacca
     6961 tcgatgactg tggtctggat catgaggtta ttgatgtgat agactcactc caaaagtcag
     7021 gtcaactaaa gatgcggctt tatgtgatgt tgagtgatgt ccgtaaaaac tacaaatacc
     7081 tgatcgacaa aggaaagatc aagacggata agctaaatgt ccgcggcttc aaaatctatt
     7141 ctgacggtgc tttgggttcc cgtggcgcct gtctgctaca cccatattcg gacaagcccg
     7201 gatggaccgg ttttctgttg agttctaaag agcattttga ctcagttgcc aatgtcatat
     7261 atacccatgg cttccagatg tgcactcacg ccatcggaga ctcggccaac agagtattgc
     7321 tggagaccta cgccaaatac cttaaaggcc caaatgacct tcgctggaga atagaacatg
     7381 cacaagtcat agatgcaaat gatttcaaat tttttagtga ttacaacata gtgccatcgg
     7441 tccagcctac ccatgcaact tcagatatgt attgggccgg agaaaggctt ggcaaagaac
     7501 gattgataaa tgcctatgcc tatcatcatc tgatggaaca gaatggctgg attccactag
     7561 ggacggactt tccggtagag gacatttcgc cattcaagac attctacgcc gccgtggtgc
     7621 gcaaagatgc aaaaggctac cctgagggtg gattccagcc cgagaacgcc ctgacgcgaa
     7681 aagaagcgct ccgtggtatg accatctggg ctgctaaaag caactttgaa gaaaatgaga
     7741 agggaagcct tgagataggg aaatgggcag attttatcat taccgaccga gatttgatga
     7801 caacggaaga aagtgatata ttagggacca aggttctgaa aactttcatt gccggagaaa
     7861 aggtctttga ggcagataaa aaataaacag tattcccgta gatgaaaaga tagtcaggtt
     7921 atgaaattct gtcaattaga atgtgacaat tgaatctttc cggcatcgat attgttggaa
     7981 agtaaattcc tgacaaaatg aaataaaaat taaatattga ataaaatgaa aaaactgatt
     8041 cttccatcct tgataatcgc cttattccta gtagcttgta acttcaacaa caaaactcca
     8101 tccaatgata ctaaaagtgt aaaattacca ccggatcctt catccattgt gtataataca
     8161 gactggatac tcaaggacat gaacggcaag gaagttaaaa tcagtcctga tgcgccaaag
     8221 aaggcaatga tacattttga caaggaagat catgttacag gtagtggggg ctgcaactta
     8281 ttttcaggta attgcaaatt tatcgacaat gaaaagattg cgatttctta tggagctgcc
     8341 acacagatgg catgtccgga aatggatata gaaagtgaat tttttgccac acttggcaaa
     8401 gctgaaaaat acaaacttga cggaaataaa ttttacctga tggataaaaa cggtaccgta
     8461 ctgctttcct tcacaaaaga ttgatgaccg gacagtttaa gctttcattt tctaattatc
     8521 aacatttctt tcgaatgaaa gagaatttcg gtttgaccat aatcaacctc tgcttaaagc
     8581 aaaccatagg ccattgatgt tctgaatcaa gtaaattttt aagattacaa agagtttgaa
     8641 gggagagaat aaaaaataca ataccgacaa aattcttgcc atgaaaaaat gcttcttttt
     8701 atttcttttt attggccttg gtacatggtc agtagcgcaa agaaatatta ttacggacca
     8761 gatcattgtc aaaattaagg attgctacct tcaaaattcc cgtgacaaat caaatttagc
     8821 tccatcgata acccaatcct ttactctatt aggagaaaga ttcaaagctc tggacacaaa
     8881 gaatctttat ttgggtaaga accacagcat ggcttacttc accattcttt tccctccagg
     8941 atcagatatc gacgaaatta taactgaata caaaaagctc gaagagattg aatatgccga
     9001 acctgattat ataggctatg gtgccggtgg cgatggattt ataccggatg ataagtttta
     9061 tgtaaaacaa tggggcttga acaacaacgg tactttccaa cacgcacagg gaatagcggg
     9121 aattgatgtc aacatggaag aatcatggtc cataacaaca ggcgacagca acattatcgt
     9181 ggccatattg gatagtggcg ggaagttgga tcatccggag tttgccggcc gcatttggca
     9241 gaatcacaag gaaatctccg gtaataatgt ggatgatgac gagaatggat ttacagatga
     9301 ttttcaggga tgggactttg taaacaaaga taataatcct tccgatgatc atggccatgg
     9361 aaccaatgtc ggaggcatag ttggctctac gggtaataat ggcaggggct ttgccggtgt
     9421 tgactggaac tgcaaactta tgttcctgaa agtattgaat tcaaacaata ccgggtatca
     9481 atcaagcttc atcgctgcca tatattatgc ggtggatcat ggtgccaggg tgattaacat
     9541 gtctataggt gggggccaat ccgatatata ccgcgatgca gtaaattatg ctttgcaaaa
     9601 aaacgtaact attgtggctt gcatgatgaa tcacaataca gaggcccctt attatccggc
     9661 tgcttttccc ggagtaatag ctgtaggctc tatcgatccg aacggtatga ggacgcagcc
     9721 atttttttgg aacagcagca gtggaagcaa ttttggaagt catatttcag ttgtggcacc
     9781 gggtaattac atttatggat tgagccattc ttccaattcg aattatgata cttattgggg
     9841 cggaacatct caagcaactc ctcatgttgc aggtgttgta tcgctgatgt tggcaataaa
     9901 tcccaatctg acgccaaagg aaatcaaaaa tattctggaa acgacggccc gtgatggaat
     9961 tggaaggccc gaagaggaca ctcctggatg ggacaaatat cacggatggg gacttatcga
    10021 ttcatacaag gccttgacat attcagtatc aagccggaaa aatccagtac attttccgga
    10081 tttttctgtt ttccctaatc cggctcgcag tgcattacag cttatctttc aaacacccgt
    10141 aacagaagga gaggtatata ttctggacct tcttggcaga caagtctatt caaatcaaaa
    10201 aataaatgga gataagttag aaattgattg cagtgaaatc ccaaccggta tctacactat
    10261 cgttcttcag aataaaggca gtactcatac cggaggcaag ttattgatca tccactaaat
    10321 tcatttaacc aattttgttc ttccattttc cttcaaaatg tgatcaccgg atgacacaaa
    10381 acactatggg taaaggattg aagatagtat ggaagaataa agcaggccag cagacataga
    10441 tggattaata aataacgtca tcttggaata taaaaaaggc cggagcaatt ccggcctttt
    10501 cacaatgaca aatgaaaata tatctatcaa ggtttctttt caacatcctt ctcaaagtca
    10561 cctgcccgga caactaccaa tttatccgct gaaataaact tattgaacgc tttattgacc
    10621 tgatccggtg tcagtgcctt aatggcatcg tcgatcttac catcaaattg catggttcta
    10681 ccaagaaaaa ggttactatt gagtgttcct gccagggatg cgtcctgtgc ccggcttacc
    10741 tggcgagatt gcaataggcc agatttggca gcttcgattt cttcagcggt aaagccttct
    10801 ttattcgctt tagtaatttc ctccttaaca caaatgttta ctctttctga gttttcagga
    10861 gcagagattg catataccat aaaacgccct gttttatcca atgcgttagc cgagaatcca
    10921 gatccgacac catagctcag cccttctttc tggcggatac gcgtggcaag tcttgagttg
    10981 agaaagcctc ctcccaaaat atagtttgcc aaaaccattg ctggatagtc cggatcagta
    11041 tctgatattt caatagaata tccggctaaa taaagagcat tggatttgtc aggtgtatta
    11101 attgtttttt cacccgcctt agtaggttga taagggcttg ctatgcgctt gtaaggcatc
    11161 tttgatttcc agttgccaaa tgtctccaag agcattttct caatttctgc ctttgaaaaa
    11221 tcacctacta ctgaagcagt agctttggat gctccataga aatcactatg aaacttcttt
    11281 agatcaccca acttgactgc cttaagcatt tcaatatttt cggataatgt tggtgtataa
    11341 cggggatcgt cttttggata gctggacata gctcttgcaa actcaattcc cgctaatccc
    11401 tgaggatcac tcatttgttc ttccaatccg ctaacttctt gcgtaaccat aatatcaaat
    11461 tcgttttgtg ggaatgacgg atttttcaaa atatccgcga caatggcaag tgttggcttt
    11521 aaattttcct tggtcgtctc tatccgaaca ttacatgaag tggcaccgcc actgaaggaa
    11581 actcttgatt taagcttgtc aaattcatcc gtaatctgtt gcttggttcg tgtctttgtc
    11641 cccttgtcaa gcattgctgc ggtaaggtct ccaatagagg atttattttt caaggaattc
    11701 aactccccaa atcttattgt caaattggca ataacgacat tgcctcttgt ctgcttgggc
    11761 aacatagcga cctcaaaatt accactcttg gatttagcaa ctgttgttct ttcatctata
    11821 ttgttcggtg aaggatcgaa agcctcacct gaggcaactg cagcatttcc tttgtaatct
    11881 tttacaactg cggcaacatc aattggcccg ggaacctcca ctcgatccgg attcttatcc
    11941 gggatgaatt ctccaatagt acggttactg tttttaaaat atttcgtagc tacggcctga
    12001 agatcttcca aggtaacttt cttaatatta tcacgattga taaacataag cctccagtct
    12061 ccctgggcga tatattcact caaacctgta cctattcttt ctacattgtt gaaggtcata
    12121 tcccaggatt tcaacatctt ctgcttttgg cggtcgagtt cttcctgtgt aaaggtcata
    12181 cccttaacct tatcaagacg gccaagtaaa atcatcttcg cttcttcaag ggacttgtcc
    12241 aaaggtacgg ttgccccaaa catcataatg ccgggttctt ttaattcaaa tccaaatcca
    12301 tccactgagg tcgctttgtt attctctacc agttccttat aaagaatgcc acttggatta
    12361 tcggtaagga ttgaagcgag gaggtctaat gctacaaagt caggatgagc acccgggctt
    12421 acatgataag ctgccataac tacctgaaca tcaccaacac gtcgtaatac gacactacgt
    12481 tccccatcct gaacaggatc aagtgtatag gttggataaa gaggcgccag ggttctgtct
    12541 ggattactta tctttccgaa atgcttctta atcagttcaa gtgttttatt tacatcaatt
    12601 ttaccggcta caaccaaaac tgcattatca ggttgatagt acttcttgta aaaggcttgt
    12661 agccgctcaa ttggaacatt ttcaatgtcc gctctagcac caatggttga cttcccgtag
    12721 ttatgccata aatatgccgt tgagatgact ctttccatca aaatacttat cggattattt
    12781 tctccgcttt caaattcgtt tctaaccact gtcatctcac tttcgaggtc tttctttgca
    12841 atgaacgaat tcaccatacg gtcagcctcc aaatcaattg cccattccag gttttcatct
    12901 gtagctgcaa aggtttcgaa ataattggtt cgatccaacc aagttgttcc atttggtctt
    12961 gcaccgtgtt ttgtcagttc ggacgggata tcagggtgat ttggtgtccc tttaaacacc
    13021 atatgttcca acaagtgtgc catcccggtc tcaccatatc cttcatggcg gctaccaact
    13081 aagtacgtaa tattaactgt gatggtcggt tttgaaggtt ccggaaaaag caacactttt
    13141 agtccatttt tggtgagtgt gtattcagtg attccttcaa ctgaaggtcc tttaacgaag
    13201 ccctctacct gagcctggat caaagaaggc aaaatcatca aagccaaacc gacgaaaaac
    13261 gccgttttca atcggtttat cctaaaaagt aaattcattt gaattaaggt taagttaggt
    13321 tataaaatga cgctaattta caaaaaagct tactttccta ttttttaatc gtcaaatgaa
    13381 gggaatcgtt cgaccaaatg acactagatt aattttacgt agtaggagaa aaactagtcg
    13441 cacattcagt tcgatggatg agaataaata ttacagggtg ttggggtaaa aatatgaacc
    13501 aggtagatga ttaataaccc aatcgtctgt aaccatgggc aatcctggtg tgcttttcca
    13561 tttatctgct ccataaagaa acgatatttt acccagctct cccgaaagtc ccatagaatc
    13621 aggagggata gttattactt tcctacctac ttcgattttc cccgcatacc aaaaattttt
    13681 cacttccgac catagcccca aataaaactt ttcatcagga actaagtaga tgattaataa
    13741 cccgaatgtc tgtaatcatg ggcaatcctg atgaactttt ccatttatct gctccttaaa
    13801 gaaacgatat tttacccagc tctcccgaaa gtcatataga atcaggtggg ttggttatta
    13861 ctttcctacc tacttcgatt ttcccacata ccaaaaattt ttcacttccg accatagccc
    13921 caactataac gtttcatcag gaactaagta gatgattaat aacccggacg ccagtaatca
    13981 tgggcaatcc tggtgagctt ttccttttaa ctgctcttta atgaaacaat tttttaccca
    14041 gctctcccga aagtcatata gaatcaagtg ggttggttat tactttccta cctacttcga
    14101 ttttcccctt tcctcccttc ttaatatctt tactcaaatt ttgtatctat gcttgccatt
    14161 gtttacgaca atccaggccc cacatctgtc ctgcatccca aaaacaggcc agtaccggca
    14221 gttggtgaaa atgacgtcct gattcgtact gtggctgcag gtgtcaaccg accggacatt
    14281 atccaacgac tcggcaaata ccccgctcct gccggcgccg ttcaggatgt tttgggattg
    14341 gaagtttcag gcttcatcga aaggataggt cggaatgtca ccagatggag acgtggtgat
    14401 gaagtttgtg cactgatccc aggtggcggc tatgccgaat acgtatcagc ccacgaaggt
    14461 agttgtcttc ctattcctgc ccatatttca ctagaagatg ccgcggccct acctgaaaca
    14521 ctttttaccg tatggcacaa tgtatttcaa cgcggtggac ttaagaaggg ggagacagca
    14581 ctaatctatg gtgcttccgg aggaattggc agcatggctg ttcaacttat ttcaatttat
    14641 ggcgctcatg catttgctct tgccggttca cctgaaaagg ccgctttttg cacatcatta
    14701 ggcgctgaaa aagtaatcga ctatacacag aataacctgc tcgatactct tttgtcagaa
    14761 tccatcgacc ttattctgga cagcctcgga ggtaaattcc tagatatcgg ccttaccctg
    14821 ctaaagcccg aaggtcgact tgtatatatc aatgcaatgg aaggtaacca accaggtctt
    14881 gatattatga aggtcatgaa aaaaagactt tttatcactg gcagtaccct caggccaaga
    14941 ggccttgatt ttaaggcttc attggctaat gatataaaac agtatgcatt cccattgctg
    15001 gaagatcaac gcttcaaaag tgttgtcaat taccgttttc cggcaaaaaa tgcctcagat
    15061 gcgcatgaat tgatggagtc aagagatttt accggcaaga tcatacttca attttaaaag
    15121 attatcttcg tacctttcaa cttttgtcaa tcttatctgt acaaactata taactatatc
    15181 agaatgagta taacacatct tgagccctct gccctctggc gtaatttctc tgctcttaat
    15241 gcagtcccgc gtccatccaa gaaagaagga agagtcatcg ctttcatcaa aaaattcggt
    15301 gaatctttaa accttgaaac tcgggtggac gaaacaggca atgttatcat cttgaaaccg
    15361 gctacaccgg gaatggaggg gcgaaaaaaa gtaatactcc aatcccatct tgatatggta
    15421 tgtcaaaaaa acaatgatac tgaatttgat ttcgataccc aaggcatccg aatggttaca
    15481 gatagcgatt gggtaatggc agaaggcacc accctcggtg cagacaacgg catcggtgtt
    15541 gcagcaatca tgtccatcct tgaaagcaag gaaattgacc atcccgaact ggaagcgctg
    15601 tttaccatcg atgaagagac gggtatgacg ggtgctttcg gtctcaagcc cggcgccttg
    15661 tccggtgaca ttcttctcaa tcttgatact gaggaagatg acgagataga cataggatgc
    15721 gcaggcggta tggatgtcac tgcaaccgga caatttatca cagaatcgta ttcaggaata
    15781 gggatccaaa tcgagatcaa gggccttcgg ggtggccact caggcataga tatccacgaa
    15841 ggccgcggca atgcaaatgt aatcttaacc cggtttttat ttcttggatt gaacgatgac
    15901 atccgtctgg taaatattaa tggaggaggc cttcgcaatg ccatccctcg tgaggctaaa
    15961 gcagtggtgg tttttaccca aggtagtcat ttcctccagc aagccgaaca attgaaagtt
    16021 gaaatcctac atgaatattt aaccctggaa cctacccttg aaataaatat ttcaccaagc
    16081 gaagtcaatg cagcgataat gaatacagct gactccggtt ccttcatccg ctccctgaaa
    16141 gttgctcaca acggagtatt taggatgagt cccacagtgc cgggactcgt agaggcttcc
    16201 aacaacatcg ctcgtgtgac tttggacgat ggcaagatca ctgtcatgaa cctcacacgc
    16261 tccagtgtcg attcgactaa acaggccgtc gcggaacaac ttagagcggc ttttgaacta
    16321 tcaagaatgg aggtgacata tagtggatct tatccgggat ggcaacctaa tccgcactct
    16381 tcgatagtag agttgatgca acgaatatat gtgaataagt ttggctcggc acccaaggtc
    16441 gttgcttgtc atgccggcct ggaatgtggt atcatcggcg cacattatcc aaagatggaa
    16501 atggtatctt tcggccctac tattcaggga gcacactctc cggaagaaag ggtcaatata
    16561 aagtccgtcg gaaaattctg gagctatttg ctggatattc tgaaagaaat accggaaaaa
    16621 taaattattc ttcttttaaa aactttgcaa ctccccccgg gattgctggc aagtatggaa
    16681 atgtgcatgg aattcactcc aaattccatt tgaattattt tcacataaaa agatgggttt
    16741 taaatacctt tgtaacggta ttttataata aatacccggc caataatcat aaaatgatcg
    16801 tagctaagac attcaaatgg gaagcagccc accgactacc ccgacacgaa ggaaagtgca
    16861 aacacatcca tggccactcc tataagatga ccgtggaatt tgaaggggaa cccaatgcaa
    16921 acggtatggt aatagatttc aatgatatca agaaaatcat ccaaccacat atcgacaaca
    16981 tcgatcattc gacaatgatt gcagagggtg acgatgaact aaaagaggtc tttgacagca
    17041 aatcatggcg atattacttg ttgccttttg actccacagc ggagaatcta tgccgatttt
    17101 ttctggataa aatcatttta gaaaacagag atttacttgc tcaatacaag ataacagcca
    17161 tcggaataaa ggtgtttgag acaggcactt cttttgccta tgcaagggct accctttgaa
    17221 ttgcggattc aatcggtttg tataaaaaat ggatttttta aaacaataca ggcataagct
    17281 attaacaggc tctggacgca accgacatta tactgattgt caatgcattg aatattcagt
    17341 taaaggtatg ataaaccttt aatctttttc acttttgtaa cggatgaggc tatgaaatgc
    17401 aataaattag taaagcctaa aacataagag agaaatacca agagtgtcgt ccttttgttt
    17461 gcattattaa agctttaaga attgtataag gaataaataa aaatggggaa tatctgttag
    17521 caatcttcag tttatcccag attacgcatt agaaaaaggg aagtcaa
//
LOCUS       DAOQMC010000015        17433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1154_length_17433_cov_4.903499, whole genome shotgun sequence.
ACCESSION   DAOQMC010000015 DAOQMC010000000
VERSION     DAOQMC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17433
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1154_length_17433_cov_4.903499"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKV31_01220"
     CDS             <1..441
                     /locus_tag="PKV31_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNB91498.1"
                     /translation="DDDYLTYRIRSTAYLGEHMRAKGVPLLYPIGGHAVYVDAGLLYP
                     HIPAHQYPGQALVCELYKIGGIRAVEIGSVMFGKYDENGTLIPAPMELVRLAIPRRVY
                     TQSHVDYVIEVFDEIIRNRDKARGLRITKEPKFLRHFTAHFEEL"
     gene            478..951
                     /locus_tag="PKV31_01225"
     CDS             478..951
                     /locus_tag="PKV31_01225"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB91499.1"
                     /translation="MSAHLKTPSYSETIKTEVVCPNDTNPMGILQGGRLVQWMDIAAA
                     VSAQTHADGICVTASIDNVKFYHSAKVGDILTIRARVTRAFNTSMEIMVEAFVRDVYH
                     NNPKPLSLAYFTFVAIDKEAKKRTVEPLVLQSDEDKRNFEAAGQRKEDVLKKKKV"
     gene            948..2189
                     /locus_tag="PKV31_01230"
     CDS             948..2189
                     /locus_tag="PKV31_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNB91500.1"
                     /translation="MSKIELLAPAGSFESLMAAIHAGADAVYFGVAQLNMRAKSINSF
                     RIEDLEEIGAICRAHRVKTCLTLNTVMYDYDMQLIRTILNEVKKHGINAVIASDFAVI
                     EMCRSMNIPLHISTQANVSNVEAARFFAPFSDVIVLARELTLQQVKAITAAIEREDIR
                     GVSGHRLRIEIFAHGALCMAVSGKCYLSLHEQNASANRGACTQNCRRPYKVIDLETGN
                     ELEIDNEYIMSPKDLCTIDILDQVADSGAAIIKIEGRGKSADYVYTVTSCYREALQAL
                     EDGTYDELKVKDWMSRLEKVYNRGYWEGYYLGRKLGEWTKNPGSAATERKVYIGKSTN
                     FFSKINVAQFEIETGKLKAGDKLLVMGRNFGVHELSFDDVRVNGVEAHEAKRGDMLTF
                     QMGYKTTGTDKLYKLVPTQDA"
     gene            2182..2400
                     /locus_tag="PKV31_01235"
     CDS             2182..2400
                     /locus_tag="PKV31_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNB91501.1"
                     /translation="MPKLIHYRDKCIGCGVCQQMQPEYWRMSKKDGKATLVGAVRKKE
                     IYIREISEDDIDLSMKVVEACPVRIIKV"
     gene            complement(2474..3508)
                     /gene="rlmN"
                     /locus_tag="PKV31_01240"
     CDS             complement(2474..3508)
                     /gene="rlmN"
                     /locus_tag="PKV31_01240"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNB91502.1"
                     /translation="MTNDIRLISPSQLEEWLKQQGEPRFRAGQISEWIWQKQALSFDE
                     MTNLPLALREKGKAEFVFRPIEEDFVQTSRDGTIKSRFKLHDGHMIESVLIPVPDEKR
                     FTACVSSQVGCSLACKFCATGMMGRIRNLDFPEIYDQVVRVNRQALEHFGHPLTNIVY
                     MGMGEPLLNYSNTLRSIDIITSPKGLGMSPSRITISTAGIAKMIRRLADDGVKTNLAL
                     SLHAANDGKRNEIMPINDQNGIDILMDALRYFYRKTRNRITYEYLALQNFNDNEEDAV
                     QLAALTRDFPVRVNIIEYNTVEGLAFHKTTEDKLDKFVRTLNQHHVNVTVRRSRGKDI
                     DAACGQLANK"
     gene            complement(3977..4930)
                     /locus_tag="PKV31_01245"
     CDS             complement(3977..4930)
                     /locus_tag="PKV31_01245"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB91503.1"
                     /translation="MVDTQHEFLQRNDHAGNDYALFYTIYQRKDIVPGATLLVLHGMQ
                     EHSGRYDEFARYMAERGFVVLTYDHRGHGKTARNMSEMGFFRKNKPAQLLLEDVRAMK
                     SLLEGQFPGLPHFVLGHSMGSFILRTFLRQEKSDFQGIILVGTGSRTPGAILIKAGLH
                     LFNVLSPKSRPSFLNNIFGTINNARFRHEVNAGATSWLSVSMDNRNAFEQDPQCGVPF
                     TVNGFLGLLTVHVRATSPEWSGNLPKDQPILFVGGSDDPIGDFGKGVLKVARHLEKAG
                     FSDTTIKLYSGMRHEILNEDIRLEVFEDILQWMGNRVRIKI"
     gene            complement(5338..6156)
                     /locus_tag="PKV31_01250"
     CDS             complement(5338..6156)
                     /locus_tag="PKV31_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91504.1"
                     /translation="MIHRKMLILLGLCLVFATSCQKTDKRDYTGTYVGHIRGKSLSMS
                     LSQPDKTTIKGTIFDRRSTYLIHGEIIGDGIKATATDTVAKTEFSIDGSWKGDTLTLM
                     MAMTKPMQTTRSFPVNFLKVVISTNQKGIDELADLGLLQSSGGRDTANGINIEANKSG
                     LNAGKVKSDSEITEHNFPGLWQIVSIKGKASTAFRQDAYYLFNSDKSILKLDRTAVPL
                     DGYYWNIGENKLFLFYKINGRMDEVRPDIIEYSNDLLRVKDGESEIELKKYTKG"
     gene            complement(6173..7729)
                     /locus_tag="PKV31_01255"
     CDS             complement(6173..7729)
                     /locus_tag="PKV31_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNB91505.1"
                     /translation="MIRIIGGLLLAGCSVLYGQNTPPVTVPLHVSHDAANHRITITYD
                     LMDVESDTATVVLQQKTGSGNFLTASGVTGEVGSGILPGEAKSIIYSYPSGTDLRNVS
                     LRLLAYDRHTPSIQEMVDKVDISNLQKMLGDIVGVRHYKSNPNHLIYVGNYINQYMID
                     HGLSIHHQDFTFSGLSFPNLIGRLEGLNNNKLCLINGHFDSAEFAPGADDNGTGVVGV
                     LEAIRVMSDYLFENNVQFTSFNLEELGLIGSINYVSKGIEPDQQIIGLINYEMLGYYS
                     DEPNSQAIPPGFELLFPNQVVEITNDQKRANFIVNTGAETSFGLMAAFTKAARTYVPG
                     LKVIELPLPGNGTIAPILRRSDHAPFWDKGYPALMITDGAETRNPNYHQLSDKIETIN
                     FDFMAKVVKTGIATLATIAKPLNGSTSGIDLSSILSTNTIGALEAITIMPNPVKDVLS
                     FNYISGTNNGKFTAEVISDTGHKILKRVMDKDASGRYSLNIGSRTAGQYHLHIIDGHN
                     RTVARFMIIE"
     gene            complement(7819..8337)
                     /locus_tag="PKV31_01260"
     CDS             complement(7819..8337)
                     /locus_tag="PKV31_01260"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNB91506.1"
                     /translation="MADFIIRPWVLADLTSLVKYANNFNISRNLTNHFPFPYTEDAGK
                     IFIEFAMAGRPNHIFAIEIDGEAAGGIGIHPQSDIFLKNAELGYWLGEPFWGRGIISN
                     AVRQMVDFGFRTFEIDRIFARPFGTNIASQKVLEKNHFKLEGRFDKVIYKNGAYQDEL
                     VYAIRRENWLKI"
     gene            complement(8483..10240)
                     /gene="aspS"
                     /locus_tag="PKV31_01265"
     CDS             complement(8483..10240)
                     /gene="aspS"
                     /locus_tag="PKV31_01265"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNB91507.1"
                     /translation="MYRTHNCGELRLSDAGNNVTLSGWVSVSRDFGGLTFLDLRDRYG
                     ITQLVFDADSNPEIREKAQKLGREYVIRVAGKVRERSNKNPNRATGEIEIDVDTLHIL
                     NASRIPPFTIEENTDGGDDLRMKYRYLDIRRDPVKNNLIFRSNLAIETRKYLADEGFI
                     EVETPFLIKSTPEGARDFVVPSRMHPGSFYALPQSPQTFKQLLMVSGLDKYFQIVKCF
                     RDEDLRADRQPEFTQVDCEMSFVTQEEILNTFEGLTRHLFQSVLQIDLGTFPRMTYDE
                     AMHRFGSDKPDMRFGMEFNYLKEAVSGRDFGVFDSAETVIGICAKGCESAFSNKDLKE
                     LTEWVQRPQIGAKGLVYVKYGADGIKSSVGKFYSEEDLLRIGKECGAETGDLLLILSG
                     ELEKTRKQLNELRLEMGRRLGLMREGDFKPLWVVDFPLLEWDEETQRFYAMHHPFTSP
                     KMADLEKMQSDDKEVLNSLRADAYDLVINGSEIGGGSIRIFDRELQAKNFRLLGFSPE
                     EAERQFGFLMNAFEYGAPPHGGIAFGFDRLCAVMSGSSSIRDFIAFPKNNQGRDMMID
                     APATIDGGQLKELGLKLDS"
     gene            10538..11956
                     /locus_tag="PKV31_01270"
     CDS             10538..11956
                     /locus_tag="PKV31_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91508.1"
                     /translation="MNDFRRSLRISDQKFGWVAVVVMAVLAVYAIVFVLERMLFLDAP
                     FMLSNIINSGSPRIMEHRYGSVITQIWPWMGMKAGLPLIWLMHLYNGAFNVFYLVVVC
                     LLVFRWRAYGAAVLMALYYTAMSDQAFFWTNNEIHQAVAWFFVWYGFYLYQRKRAGLR
                     RKRFIWFGMTATIAAVTHPLMVPVLAFTWIFLLMTEGRQTGDGKVRVGLTMMIVMACC
                     IRIWFSLRAGWYDRDKVETVKTNLLSGITEVFDKQLAAQLLHQYSHGLIVGAVLGLAG
                     LFLLMVRRKYLLSLMYLLVIVSYHLLFLSAFDTWVDFYSQSEMMPLTIIYGLPVVRFL
                     PEYIGSRLLFSITVMVFPIRLIMIPVASVPFTKRVSNTMSLIREMKEKNITKALIPDN
                     KDLESIYIMTWGLPIETVLASAISGDRQRTVKPLPADKLEEAVQTPSDQFLDCFRIIP
                     INTMNPYYFNLDSVGRYEVVRQ"
     gene            complement(12239..12625)
                     /locus_tag="PKV31_01275"
     CDS             complement(12239..12625)
                     /locus_tag="PKV31_01275"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB91509.1"
                     /translation="MEISEYIAQRSSGEKAICELLATTIDKCLRQSQSKIWHGHPVWF
                     LDGNPIVGFSSQKAGIRLMFWSGADFEEDGLNILGKKFKDASIFYNDVADVSIKDLER
                     WLTKAEAIQWDYKNLVKRKGRLEKLR"
     gene            complement(12684..13121)
                     /locus_tag="PKV31_01280"
     CDS             complement(12684..13121)
                     /locus_tag="PKV31_01280"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB91510.1"
                     /translation="MASINPYIHFNGNAEEAFTFYKSVFGGEFATLVRFKDLNFEGSI
                     STEKEAEKIMHIALPIGEHDLLMGSDTPEILGRHNEHENRSKISVSAESKQEAERIFY
                     ALSEGGQVEMPIEDSPWGTYFGMFRDRFGIEWMVEYNPQVFVR"
     gene            complement(13146..13574)
                     /locus_tag="PKV31_01285"
     CDS             complement(13146..13574)
                     /locus_tag="PKV31_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91511.1"
                     /translation="MTKIAMMPVLWFHVSDGETDPVTNYCNCLFWISLNIEAGIPLGG
                     TAEGMNEMWDLSLFEHRILSGTVVRKRHKFKDSFACRIFFRDQQEIDPRRSYHTTAGA
                     ESRCGLFFDKFSLPWKAIPQGMGISQAGSMHPVHCQTQQE"
     gene            complement(13591..14001)
                     /locus_tag="PKV31_01290"
     CDS             complement(13591..14001)
                     /locus_tag="PKV31_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91512.1"
                     /translation="MTKQIFINLAVNDLSQSMDFYTNLGFTNNPLFSDDTAKCMVWSD
                     SIFVMIMTHEKFESFATKPIADTKNTLAGLFSLSVESLEEMDRIIEAGLKAGGVEPNE
                     MRDYGFMQQRTIEDFDGHTWEIFYMDMSKFPQGA"
     gene            complement(14029..14427)
                     /locus_tag="PKV31_01295"
     CDS             complement(14029..14427)
                     /locus_tag="PKV31_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91513.1"
                     /translation="MKHKILNLLLIITSLLGYLEWSGDSRSFLFQAEGEILYKLFTDP
                     ASTIHPFILLPLLGQLLLLVTLFRKRPGKVLTYIGISGLGLLLGFMFVVGIISLNIRI
                     IASTLPFLLVALYTIYCLRGTKSLANSMYL"
     gene            complement(14429..14842)
                     /locus_tag="PKV31_01300"
     CDS             complement(14429..14842)
                     /locus_tag="PKV31_01300"
                     /inference="COORDINATES: protein motif:HMM:NF039673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HNB91514.1"
                     /translation="MKIYTTNYFNTFILVAADCPVLTGEVPPYRGDTKSAANIQYELI
                     NNNPYRYTSDDVLFQVFALKNNIEEKDRESAREQFFAKGQACFRTSPLTKRYGWGVHF
                     DGEGRMAIYSFSSAAYEMFALDTSLKVIRAMKSSR"
     gene            complement(14870..15328)
                     /locus_tag="PKV31_01305"
     CDS             complement(14870..15328)
                     /locus_tag="PKV31_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB91515.1"
                     /translation="MRPLEKTIQEILDNIPTERKEAFHRLHHVIVDNLPEGFEVGISY
                     GMLGYVVPHSIYPAGYHCKKEEPLPFASLASQKNSINFYHMGIYADPGLLEWFVTEYP
                     KYCKSKLDMGKSCIRFKKPDDIPFGLIGALMRKMTVAQWINTYETAFRKK"
     gene            complement(15339..15842)
                     /locus_tag="PKV31_01310"
     CDS             complement(15339..15842)
                     /locus_tag="PKV31_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB91516.1"
                     /translation="MRKGILIIAGIVTSFVLGYFLNFAYALTSGNKEEQSKKVEVTDN
                     PKLGAFSVSLSVRDLKASKEFYEKMGFSILGGSMEKNYLIMKNERALIGLFQGMFQGN
                     ILTFNPGWDENGQNVKKFDDIRVIQRRLKQSGLTLTSEAEEGSTGPASLTLTDPDGNI
                     ILIDQHR"
     gene            16682..>17433
                     /locus_tag="PKV31_01315"
     CDS             16682..>17433
                     /locus_tag="PKV31_01315"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB91517.1"
                     /translation="MRSALFLLIFVLGALTAHSQNYRILGVVNDQIDSSSLIGASVFL
                     KSLPDSNTVAVATTDAGGRFEFKDLKAGPYVIQSTYVGYKTFSKAVELGFSAAFSIEI
                     MMRGDGITLGGMEVIGRTPPVTVKEDTSEIRANQFKVNPDANAEDLVTKMPGIQKVDG
                     KIQAQGEEVKRVLVDGAVFFGSDPSATLKNLPAEMIDKVQVFDKASDQAQFTGISDGN
                     EEKTINIVTKPDYRQGKFGRVFAGYAPDDKYKA"
BASE COUNT         4602 a         4087 c         3765 g         4979 t
ORIGIN      
        1 gatgacgatt atcttaccta caggatcagg agtacggctt atctgggcga acacatgcgt
       61 gccaagggcg tacccctgct ttatcctatt ggtggtcatg cggtgtatgt cgatgccggt
      121 ttgttgtatc ctcatattcc ggcacatcaa tatcccggac aggcgttggt ctgcgaattg
      181 tacaagatcg gtggcatccg tgctgttgaa atcggatcgg tgatgtttgg caaatatgac
      241 gaaaacggga cgctgatacc ggcaccgatg gagttggtcc gcctggcgat tccgcgaagg
      301 gtatatactc agagtcatgt cgattatgtc attgaggtat tcgatgagat catacgcaac
      361 agggataagg ccaggggcct ccgtatcacg aaggaaccta aattcttacg gcattttacc
      421 gcacattttg aagaattgta attgcccgac attgttgtcg aatcaaaaca tcaggatatg
      481 tcagcacatc tgaagactcc gtcttattca gaaacaatca agactgaagt ggtatgcccc
      541 aacgatacca atccaatggg catccttcag ggtggtcgcc tggtccagtg gatggacatt
      601 gccgctgccg taagtgctca gacccatgcc gacggtatct gcgtcactgc ctccatcgac
      661 aatgtcaagt tctaccactc ggctaaggtt ggagacatcc tgaccatccg ggccagagtc
      721 accagagctt tcaacacatc catggagatc atggtggagg catttgtccg ggatgtttat
      781 cacaacaatc ccaaaccttt gagtctggca tattttacat ttgtcgccat cgacaaagaa
      841 gctaaaaaaa ggacggtcga accgcttgtt cttcagagtg atgaggacaa aaggaacttt
      901 gaagcagcgg gtcaaaggaa ggaggacgtc ctgaaaaaga aaaaagtatg agcaaaatag
      961 aattacttgc acccgctggc tccttcgaga gccttatggc agccatacat gctggtgctg
     1021 atgccgtata tttcggtgtt gcccagctga atatgcgcgc caagtccatc aactccttca
     1081 ggattgagga tctggaggaa atcggtgcaa tctgcagagc gcatcgcgtc aagacctgcc
     1141 tcacactcaa cacggtcatg tacgattacg atatgcagct catccgcacc atcctgaatg
     1201 aggtgaagaa acacggtatc aatgcggtga ttgcatccga ctttgccgta atcgaaatgt
     1261 gccgtagcat gaatattcct ctgcatatct ccactcaggc caatgtaagc aatgtggaag
     1321 cagcaaggtt tttcgcacct ttttcagatg tgatcgtact cgcacgcgaa ctcaccttgc
     1381 agcaagtcaa ggccatcacc gctgccatag aacgcgaaga catcagaggt gtatccggcc
     1441 atcgactcag gattgaaatc ttcgctcacg gggccttgtg tatggcggtt tccgggaaat
     1501 gctacctcag cctgcacgag cagaatgcct ctgccaaccg cggtgcctgt acacaaaact
     1561 gccgtcggcc ctataaagtc atcgacctgg agaccggcaa cgaactggaa atagacaacg
     1621 aatacatcat gtctcccaag gatctttgca ccattgacat cctggatcag gtagccgatt
     1681 caggtgctgc aatcatcaag atagaaggtc gtggcaagag cgccgactat gtctatactg
     1741 ttacgtcttg ctacagagaa gcacttcagg cccttgaaga tggtacctat gatgagctta
     1801 aggtcaaaga ctggatgtca agacttgaaa aagtctataa ccgcggatac tgggaaggat
     1861 attatctggg cagaaagctc ggcgaatgga caaaaaatcc gggctccgca gctacagaaa
     1921 gaaaggtcta catcgggaaa agtaccaatt tcttctcaaa gatcaatgtt gcacaatttg
     1981 aaatcgaaac cggcaaattg aaggcaggag ataaattgct ggtgatggga agaaatttcg
     2041 gggtacacga attgagtttc gacgatgtac gcgtcaatgg tgtcgaagct cacgaagcca
     2101 aacgtggcga catgctcacc tttcagatgg ggtataagac caccggtacg gataagttgt
     2161 acaaattggt ccccacacag gatgcctaaa ttgattcatt atcgggataa atgtattggt
     2221 tgtggtgtct gtcagcagat gcaaccggaa tattggcgta tgtcgaagaa ggacggcaag
     2281 gccaccctcg ttggtgctgt ccgcaaaaag gaaatttata tccgggagat tagtgaagat
     2341 gatatcgacc tgtccatgaa ggtcgtcgaa gcatgtccgg tacgcatcat caaagtgtga
     2401 tcattgccca atacttactc tgaattttat tgtcggtatc gcaatattca ttttaccttt
     2461 atctgtcagg ctctcactta ttcgccagct gaccgcatgc agcgtcaatg tccttaccgc
     2521 gactgcggcg tacggtgaca ttgacatgat gctggttaag cgtccggaca aatttgtcca
     2581 atttgtcttc tgtggtttta tggaaggcca gaccctccac cgtgttgtat tcaatgatgt
     2641 tgacacggac cgggaagtcc ctggtcaatg ccgccagctg aacagcatcc tcttcattat
     2701 cgttgaaatt ctgaagggcc agatattcgt aggtgatgcg gttcctggtt tttcgataga
     2761 aatagcgcag tgcatccatc aggatgtcta tgccgttttg gtcattgata ggcatgattt
     2821 cgttcctctt gccatcattg gcggcatgaa gactcaatgc gagattagtc tttacaccat
     2881 catctgcaag cctgcggatc atcttggcaa tacccgccgt actgatggtt atccgggaag
     2941 gcgacatgcc cagccctttt ggagatgtga tgatatcgat gcttctgagc gtgttggagt
     3001 agttgagcaa tggctctccc atccccatat atacgatatt ggtcagcgga tggccaaagt
     3061 gttccaatgc ctgccggttg acccgaacca cctgatcgta aatctcggga aagtccagat
     3121 tccgtatgcg ccccatcatt cccgtagcac agaatttgca tgcaaggcta cagcctacct
     3181 gggaggaaac acaggcagta aaacgttttt cgtccggaac cggaatgagt actgactcaa
     3241 tcatatgacc gtcatgcaac ttgaatcttg acttgatggt accatcccgg cttgtctgca
     3301 caaaatcctc ttcaatcggc ctgaatacga attctgcttt ccccttttcc cgcaaagcaa
     3361 ggggaagatt cgtcatttca tcgaaactca aggcctgttt ctgccatatc cactccgata
     3421 tttggccggc acggaaacgc ggttcacctt gctgtttgag ccattcttcc agttgggaag
     3481 gagagataag cctaatgtcg ttggtcatag attctgtgga ttaaatgcaa agataaaaca
     3541 atgatggtat tgcgcctgca atgtttaaca agctaactat tgcaaccgat atttacttat
     3601 aaaccttaat cccaaaaatg agctcccagg ttcttgtaac cactccctca aatcagttga
     3661 attgtacgtg agattcgaat ttcggatttt ccctgaacat agcctgacaa accctgtcaa
     3721 tgtcttatgc cagatatctt tcacgttatt tattacccgg aaaaaatact tttattcatt
     3781 tactgctatc agaaaattgc atctgagaca gacccttgtt gttaccattg ttgccgaatc
     3841 atcacgctca ggatacatgc ctcaaaacca ttcctgtatt tttctacact taatattcca
     3901 ttccttaata tctacatggt caattaaaga aggttactta ctatttatcc cctttttact
     3961 cccggaaata ttgttatcag atttttatac gaactctgtt tcccatccat tgcaggatgt
     4021 cctcaaaaac ctcgagtcgg atatcctcat tcaatatttc gtgacgcatt ccagaataca
     4081 attttatggt agtgtctgaa aatcctgcct tctccaggtg tcttgccact tttaggacgc
     4141 ctttgccaaa gtctcctatg ggatcatccg acccgcctac aaataaaatc ggctgatctt
     4201 taggcaggtt acctgaccac tcgggactcg tggcgcggac atggacagta agaaggccca
     4261 ggaagccatt gacggtaaat ggaacaccac actgtggatc ctgttcgaaa gcattccggt
     4321 tgtccatact gacactcagc cagcttgtgg caccggcatt gacctcgtgc ctgaaccggg
     4381 cattattgat cgtcccgaag atgttgttga ggaaggaagg gcggcttttc ggtgaaagca
     4441 cattgaacaa gtgtagccct gccttgatga gaatggcgcc gggtgttctg cttccggtac
     4501 caactaagat gatgccttga aaatcggact tttcctgtct gagaaaagtc ctcaggataa
     4561 atgagcccat ggaatgacca agaacaaagt gcggtagtcc cgggaattgg ccttccagca
     4621 aagatttcat ggcacgaaca tcctcgagca aaagctgtgc aggtttgttt tttctgaaaa
     4681 atcccatctc agacatattc cgggcagtct ttccatggcc gcgatggtca taggtcaata
     4741 ccacaaaacc ccgctccgcc atatatcgcg cgaattcgtc ataacgtccg ctgtgctcct
     4801 gcatgccgtg gagtaccagc aaggtggctc ccggcacaat gtccttcctt tgataaatag
     4861 tgtaaaagag ggcataatca ttgcccgcat ggtcattgcg ttgcagaaac tcgtgttggg
     4921 tatcgaccat ctttgctgtt ttatttactg atacaaaata ttttaaaaat tgtttgccgg
     4981 cgacaatatg gtttaactac tccatctttt agttggcttg cagtataaat ctcattctac
     5041 agccatgttt atcacttccg gcagaagggt gtagtttgtg ctattgatac cagggcatta
     5101 aggagcgttg agggcctccg taaccacctt catttctcag gatcaggacc tgtcgacagg
     5161 cacggcggtt aaatggtccc ttctgtcgtt ttgcccattg ggccggttga ataaaaccga
     5221 ttgatactgc caatgatcaa ccgggcatct acggcgatca aagaaaacaa aattcaacta
     5281 ccctactggg ctatatatag ggatgaaggg ggatgaatac ttccccggcg ggccctgtca
     5341 gcctttggtg tattttttca attcaatctc agattcgcca tcctttactc tgagcaggtc
     5401 attgctatat tcgatgatat caggacgaac ttcgtccatt ctgccattga ttttataaaa
     5461 cagaaacaat ttgttttccc cgatattcca ataatatcca tccaaaggaa cagctgttcg
     5521 gtccaatttc aaaatgcttt tatcgctatt gaacaagtaa taggcatcct gtctaaatgc
     5581 cgtggaagcc ttccccttaa tacttacgat ctgccataat ccggggaagt tgtgttctgt
     5641 aatttctgag tcacttttga cttttcctgc attcaatccg gatttgttgg cttcaatatt
     5701 gatgccgtta gctgtatccc ggccgccgga agattgtaaa agtcccaggt cggccaattc
     5761 atctattccc ttttgattgg ttgaaataac cactttcagg aaattgaccg gaaatgacct
     5821 tgtagtctgc atgggctttg tcatagccat catcaatgtc aaagtatctc ctttccagct
     5881 tccatcaata ctgaattcag tttttgccac ggtatccgtt gcggtagcct tgatgccatc
     5941 gccgattatt tcaccatgaa tcagataggt ggatctgcgg tcaaaaatcg taccttttat
     6001 ggtagtttta tccggctgcg aaagtgacat ggacaggctc tttcctctaa tatggccgac
     6061 ataggttccg gtataatctc ttttgtccgt tttttggcac gaggtcgcaa ataccaggca
     6121 taaccccagt aaaatcagca tttttctatg tatcaatatc tatcagtttt tgttattcaa
     6181 tgatcataaa acgtgcgaca gtccggttat gtccatcgat gatatgaaga tggtattgtc
     6241 ctgctgtccg gctccctata ttcaggctat atctacctga ggcgtccttg tccataaccc
     6301 ttttcaatat tttatggccg gtgtctgaga tgacttccgc tgtaaatttt ccgttatttg
     6361 tgccggagat atagttaaaa ctgagcacat ccttcaccgg attgggcata atggtgattg
     6421 cttccagggc tccgatggta tttgttgaaa ggatggaaga aagatcaatc cccgaagtcg
     6481 atccgtttaa tggttttgca atggtggcca gtgttgcaat gcctgtctta acaaccttgg
     6541 ccataaaatc aaagttgatg gtctctattt tgtctgacag ctgatggtag tttggattgc
     6601 gggtttcggc gccatcggta atcatcaggg ccggatagcc tttatcccag aagggtgcat
     6661 ggtcgcttct gcgcaggata ggagctattg taccattgcc cggcaatggc aattcgatca
     6721 ccttaagtcc gggaacatat gtcctggcag ctttggtaaa ggcggccata aggccaaatg
     6781 aagtctccgc tccggtattg acgatgaaat tggcacgttt ctgatcgttt gtgatctcca
     6841 caacctgatt gggaaagagc agttcaaatc ccggcggaat agcctgactg tttggctcat
     6901 cggaataata acccaacatc tcataattga tcagaccgat gatttgctgg tccggctcaa
     6961 tccccttcga tacgtaattg atacttccaa taagtcccaa ttcttccaag ttaaaggagg
     7021 taaactgaac attattttca aacaaataat cactcatgac gcggatggct tcgaggacac
     7081 ccactacgcc ggtaccgtta tcatcagctc ccggggcaaa ctcggcagaa tcaaagtggc
     7141 cattgatcag gcacaattta ttgttgttga gcccttccag tctgcctatc agattgggaa
     7201 aagacaagcc gctaaatgta aaatcctgat gatggatgga cagtccatgg tcgatcatgt
     7261 actgattgat ataattgccg acataaatca ggtgattggg attggactta taatgtctca
     7321 caccgactat atcgccgagc atcttttgca ggttggagat atctacctta tccaccattt
     7381 cctgaataga tggtgtatgc cggtcataag ccagcaaacg aagagaaaca ttcctgaggt
     7441 cagtccctga aggatacgaa tatattattg acttggcttc tccaggaagg atacctgatc
     7501 caacctcgcc ggttactccg gatgccgtga ggaaattacc ggatcctgtt ttttgttgca
     7561 aaactactgt agctgtgtcg gattccacat ccatcaggtc ataggtaatt gttatgcggt
     7621 ggttggccgc atcgtggctc acatgcaatg gtactgttac agggggtgtg ttctgcccat
     7681 ataaaacaga acaaccagct aacaacaatc ctccaatgat tcttatcatg gttttaaatt
     7741 tttataacta aggtaataca ttttcgaaaa atagccggtt tatttacctt tcctttgtac
     7801 cactacgcat tcctgtttct atatctttag ccaattttcc cgccggattg catataccag
     7861 ttcatcctga tacgctccat ttttataaat aaccttatca aaacgacctt ccagcttaaa
     7921 atggttcttt tccaaaacct tctgtgatgc gatatttgta ccaaagggcc tggcaaatat
     7981 tctgtcaatt tcaaaggttc tgaatccaaa atcaaccatt tgcctgacag cattgcttat
     8041 gattcctctg ccccagaaag gctcgccaag ccagtatccg agttcggcat tctttaaaaa
     8101 gatatcgctt tggggatgaa ttccaatgcc tcctgctgcc tcaccatcga tttcaatagc
     8161 aaagatatga tttggcctgc cggccattgc aaattcgatg aatatcttac ctgcatcttc
     8221 cgtatatgga aaggggaaat gattggtcag gttcctggag atgttgaaat tattggcata
     8281 cttcaccaaa ctggtcaaat cagcaaggac ccagggccta atgataaagt cagccataat
     8341 attctatttt tggtatcaga ttagtgcaca ccatccacaa tatctcttca tcgctaccgg
     8401 ccgaaggact aaataccgat ttccaacttc ccaatacggc taatggctgc ctgctgatcc
     8461 caaattttcc taaacccacc tcttaagagt ccagcttgag tcccagttct ttcaactgac
     8521 cgccgtcgat ggtcgcaggc gcatcaatca tcatgtcccg cccctggttg ttcttcggaa
     8581 aggcgataaa gtcacggata ctgctgctac cgctcatgac cgcgcaaagc ctgtcgaaac
     8641 caaatgcgat gccgccatgt gggggtgctc cgtattcaaa ggcattcatc aggaagccaa
     8701 attggcgctc cgcctcttcc ggactaaagc ccagcaaacg gaaattcttc gcctgcaact
     8761 ccctgtcgaa gatccgtata gacccgccac cgatctccga accgttgatg accaggtcgt
     8821 aagcatcagc acgcaggcta ttgagcacct ccttatcatc ggactgcatc ttttcaaggt
     8881 cagccatctt gggggatgtg aatggatggt gcatggcgta gaatcgctgt gtctcttcat
     8941 cccactccaa taacggaaaa tccacgaccc acaacggctt gaagtcacct tcccgcatca
     9001 ggcccaggcg acggcccatc tccaaacgca gttcattgag ttgctttcgg gtcttttcca
     9061 attcgcccga caggatcaac aaaagatcgc ctgtctctgc cccacattcc ttgcctatgc
     9121 gcagcaggtc ttcttcggaa taaaacttgc ccacagaaga cttgatgccg tcggcgccat
     9181 atttgacata taccaggcct ttggcgccaa tctgtggtcg ttgtacccat tctgtcagct
     9241 ccttcaaatc cttgttggag aaggcggact cacagccctt ggcgcagatg cctatcaccg
     9301 tttctgcact gtcaaacaca ccgaagtcac gaccacttac cgcctctttc agatagttaa
     9361 attccatacc gaaacgcata tcgggtttgt cggaaccaaa acggtgcatt gcctcatcat
     9421 aagtcatccg cgggaatgtg cccaggtcta tctggagtac actttggaag aggtgccggg
     9481 taaggccttc aaaagtgttc aggatttcct cctgtgtgac aaaggacatc tcacagtcca
     9541 cctgtgtaaa ttcaggttgg cgatccgccc tcagatcttc atcgcgaaaa cacttgacaa
     9601 tctgaaaata tttatccagt ccgctgacca tcaacaattg cttgaaggtc tgtggcgact
     9661 gtggcagcgc atagaatgag cccgggtgca ttctggacgg aaccacgaaa tcgcgggcac
     9721 cctcaggtgt tgattttatc aggaatggtg tctcgacttc aataaagcct tcatcggcca
     9781 gatattttct ggtttcaata gcaagattcg acctgaatat caagttgttc ttgacagggt
     9841 cgcgtctgat atcgaggtag cggtacttca ttctaaggtc gtctccacca tctgtattct
     9901 cttcgatggt aaatggtgga atcctggatg cattgaggat atgcaatgta tcgacatcta
     9961 tctcgatctc accggttgca cggtttggat tcttgttgga gcgttcacgt accttaccgg
    10021 ccacccggat gacatattcg cggccgagtt tctgggcttt ctccctgatt tccggattcg
    10081 agtcagcgtc aaatacaagc tgggttatgc catacctgtc ccgaaggtcc aaaaaagtca
    10141 gaccgccgaa gtctctgctt acgctgaccc agccacttag agtcacatta tttccggcat
    10201 ccgaaagtct taattcacca caattgtgcg tcctgtacat agtttttctt ttgaaggcgc
    10261 aaaattacgt tttatagtgc tgtaaaaaaa ttataagaaa aggaaatttg agattgattg
    10321 ggtgatttcg ccttttcgat tcttacacgg gaatttattg tgtctttctt ccatgattgt
    10381 cagaatcccg gaggaaaaga tttttgtgcc ttagggactt cggtcaccaa ttacaatcct
    10441 ccttcaccgg cccgcatctc agctatcttc cggcttatgg caaagagaat caacttcaat
    10501 aaaataacta tcttacactt caaatggcat taattgcatg aatgatttcc gaagatcgtt
    10561 gcgcatatct gaccagaaat tcggctgggt ggccgttgtt gtaatggctg tcttggcggt
    10621 atatgccatt gtttttgtgt tggaaaggat gttgtttctc gatgcgccat ttatgctatc
    10681 caacatcatc aacagcgggt ctccccggat tatggagcac cgatacggct cggtcatcac
    10741 acagatatgg ccatggatgg gcatgaaggc cggcctgcct ttgatctggc tcatgcacct
    10801 gtacaatggg gcgttcaatg tgttctacct tgtcgtagta tgccttcttg ttttccgctg
    10861 gcgcgcctat ggcgccgccg tcttgatggc actttactat acagccatgt ccgatcaggc
    10921 ttttttctgg accaacaatg agattcatca ggccgttgcc tggttttttg tatggtatgg
    10981 cttttatttg tatcagcgga agagagcagg acttcgccgg aaaagattca tctggtttgg
    11041 gatgaccgca acaattgcgg ccgttacgca tccactaatg gtgcccgtgc ttgcttttac
    11101 ctggattttc ttgttgatga cggaaggaag gcagacaggc gatggtaagg tccgcgtggg
    11161 gctgacgatg atgatagtca tggcatgttg cattcgtatt tggtttagtc tgcgtgcagg
    11221 atggtatgac cgggacaagg tagagaccgt gaagaccaac ctgctttcgg gcataactga
    11281 agtcttcgac aagcagttgg ctgctcaatt attgcatcaa tatagccatg gcctgattgt
    11341 aggagccgtt ctgggcctcg ccggcctatt cctgttgatg gttcgaagga aatacttact
    11401 ttctttgatg tatttgttgg tcatcgtgtc ttaccacttg ttgtttttgt ctgcctttga
    11461 cacctgggtg gacttctatt cgcagagtga gatgatgcct ctgaccatta tttacggatt
    11521 gccggtggtt cgctttttgc cggaatacat tggatcgagg ctattgttca gtattacggt
    11581 tatggtcttt ccgataagat tgattatgat tcctgttgca tctgttcctt ttacgaagcg
    11641 tgtgtcgaat acgatgtcgc ttattcgcga aatgaaggaa aagaatatca ccaaagcatt
    11701 gatcccggac aataaagatc ttgagtcaat ctatattatg acatggggcc tgccgatcga
    11761 aaccgtcctg gcatccgcta tctccggaga ccgccaaagg accgttaagc ccctgcctgc
    11821 cgataagctg gaggaagctg ttcaaactcc ctcagaccaa tttctggatt gtttcaggat
    11881 tattcccatc aacaccatga atccttacta ctttaatctg gattctgtag ggcgatatga
    11941 agtggtccgg caataaactg cattctgtct gaaaaggaaa tttatcgaat caaagattcc
    12001 gtccacatac gcccggtcag cttgtgccca caatgcatat agtccgataa accataggat
    12061 acctcatcct attaatagtg gtccaagctt gaatacatcg attggaaatt gattaaaaag
    12121 gttggttcgg gtgccgggca ttcaggcccg aaatgcttac cttacaataa tacatagatt
    12181 ccatccgaca gcatataccc acccattaaa aagccccaaa tgtttagtaa attttcactt
    12241 atcttaattt ttctaaccta ccctttctct ttactaaatt tttataatcc cattgaatgg
    12301 cttctgcctt tgtcaaccaa cgttccagat ctttgatgct gacatcggca acatcattgt
    12361 aaaagatgga tgcatccttg aatttcttcc cgaggatatt cagtccatct tcttcaaaat
    12421 ctgcaccact ccaaaacatc agacgtattc cggctttttg agagctgaag ccaacaatcg
    12481 gattaccatc cagaaaccaa acgggatgcc catgccaaat cttactttgg ctttgcctca
    12541 gacatttatc aatcgtcgta gccagcaact cgcatatggc tttttctccg gacgaccttt
    12601 gagctatata ttcagatatt tccatcgata acacacagtt agatatgata tactacctgt
    12661 tctacaattt tgatgattgg ttgttaccgg acaaaaactt gcggattgta ttccaccatc
    12721 cattcaattc caaacctatc gcgaaacatt ccgaagtatg ttccccacgg actgtcttca
    12781 ataggcattt ccacctgccc tccctcagaa agtgcataaa aaattctttc tgcttcctgc
    12841 ttgctttcag cgcttacgga tatcttgctc cgattttcat gctcgttgtg tctgcccaat
    12901 atttcaggag tatcacttcc catcagcaga tcgtgctcgc caatcggcaa agcgatgtgc
    12961 atgatttttt cggcttcttt ttctgtgctt atgctgcctt caaagtttaa atctttaaaa
    13021 cgcaccaaag ttgcaaactc gcccccaaaa acggatttat agaaagtaaa cgcttcttct
    13081 gcatttccat tgaagtgaat gtacggattg atggatgcca tatttttatt atttagtttg
    13141 attgattact cttgttgggt ctgacagtgc acagggtgca ttgagcctgc ttgtgatata
    13201 cccatgcctt ggggtatagc cttccagggc aggctgaatt tatcaaagaa taggccacac
    13261 cttgattcag cacctgctgt tgtatgataa ctccggcgtg gatctatctc ctgctgatcc
    13321 ctgaaaaaga tcctgcaagc aaatgaatcc ttgaatttat gacgtttcct gaccacagtc
    13381 cctgataaaa ttctatgttc aaacaggcta aggtcccaca tttcgttcat cccttccgct
    13441 gttccaccta atggaatacc cgcctctatg ttcaaactaa tccagaaaag gcaattacaa
    13501 tagtttgtca caggatctgt ttctccatca gagacatgaa accataatac aggcatcatt
    13561 gcaatcttcg tcatatatca atgcttttac ttaggctccc tgagggaact tgctcatgtc
    13621 catgtagaat atttcccacg tatgtccgtc aaagtcctcg attgttcgtt gttgcatgaa
    13681 gccatagtcc ctcatctcat taggctccac acctccggct ttcaatccgg cttcaataat
    13741 ccggtccatt tcctccaggc tttcaaccga aagagaaaac aatcctgcta aagtgttctt
    13801 ggtatcggca ataggtttgg tagcaaagga ttcaaatttt tcatgggtca taatcatgac
    13861 gaaaatagaa tcgctccaca ccatgcactt agccgtatcg tccgaaaata gtgggttatt
    13921 ggtaaatccc agattcgtgt aaaagtccat tgattgggac aagtcattaa ctgccagatt
    13981 gatgaagatt tgtttagtca ttatttttaa tttcaaggtt attgattctt ataagtacat
    14041 gctgttcgcc aggctctttg ttcccctgag acaataaata gtatatagcg ctaccaatag
    14101 aaacggtaac gttgaggcta ttatcctgat attcagactt atgattccaa caacaaacat
    14161 aaagccaagc aacagcccca gtcctgatat gccgatataa gtaagcacct tgcccggccg
    14221 cttccggaat aaggtgacga gcaacaaaag ttgacccagc agtggcagta gaatgaatgg
    14281 gtggatggtg gaagcaggat ctgtaaataa tttatagagg atctctcctt ctgcttgaaa
    14341 aaggaaagaa cggctgtctc cactccattc cagatagccc agtaaggaag taataatcag
    14401 tagtaaatta agaatcttat gtttcatatt atctgcttga tttcatggcc ctgatgacct
    14461 ttagagacgt atccagagcg aacatttcat aagctgcact gctgaaacta tatatcgcca
    14521 tcctcccctc accatcaaag tggacacccc aaccatagcg cttggtcagg ggagatgtcc
    14581 ggaaacatgc ctgtcccttt gcaaaaaatt gctccctcgc cgactcacga tctttttctt
    14641 ctatattatt cttcaaagca aatacctgaa acaggacatc atctgatgta tacctgtagg
    14701 ggttgttgtt gattaattca tactggatat tggctgcgct tttagtgtca cccctgtatg
    14761 gtggaacctc gcccgtcagg acaggacaat ccgctgcaac gaggatgaag gtgttgaagt
    14821 aattcgtcgt gtatatcttc ataaaattca agatttcgtc aagagggaat cactttttcc
    14881 tgaatgctgt ttcataagta ttaatccatt gtgctacggt catcttacgc atgagggcac
    14941 cgattaatcc gaaagggata tcgtcaggtt tcttaaagcg aatacagctt ttgcccatat
    15001 ccaatttact cttgcaatat ttcggatatt ctgtcacaaa ccactctagc agtccgggat
    15061 cggcatagat gcccatgtga taaaaattga tcgaattttt ctgtgaggcc aatgaggcaa
    15121 aagggagcgg ttcttctttt ttgcagtgat atccggcagg atatatgctg tggggcacca
    15181 catagccaag cattccgtag cttatcccta cttcaaatcc ttcgggcagg ttatcaacga
    15241 tgacatggtg cagtctatga aatgcctctt tcctttctgt gggaatatta tccagaattt
    15301 cctgtattgt cttttcaagt ggcctcatga gtatattatt atcggtgttg atcgatcaga
    15361 attatatttc catcaggatc ggtcaatgta aggcttgccg gacccgtgga tccctcttcg
    15421 gcttcgcttg tcaatgtcaa accggattgt tttaaccgcc tttgaataac cctgatatca
    15481 tcaaacttct ttacattctg tccattttca tcccacccgg ggttgaatgt aaggatgttg
    15541 ccctggaaca taccctgaaa aaggcctatc aacgcccgtt catttttcat tatcaggtaa
    15601 ttcttctcca tgcttccacc tagaatgctg aaacccattt tttcgtaaaa ctctttggaa
    15661 gctttcaagt ccctgacact aaggctcact gagaaagctc cgagttttgg attgtctgtc
    15721 acttcaacct ttttcgactg ctcctcctta ttacctgaag tcagagcata cgcgaaattc
    15781 agaaaataac ccagaacgaa ggaggtaaca attccggcaa tgattaaaat tcctttccgc
    15841 atgatattta tcaatttttt gaatgaaaaa attaaagaaa aaagtaaaat ctcctggcga
    15901 gactaatcat aaactgctca gggcattgtt ttaaataacc ggcataaatt taccagaata
    15961 gtattaaaaa ctataaataa tttcgacgaa ggaaagctta caaatcataa atttgaatca
    16021 atctgttcca gggaatattt cggagatatc attcaatagc tgtattcccc tgtaaaaaaa
    16081 cagatctatt ataaccatat cgtgaccaat tggctcatat cattccaaat gttataccaa
    16141 gatgatttta tataatagtc cataaattct tggtatacct ttccggacag ctatgttact
    16201 agagctctgc tctgtcccgg gagcaaaaca atccggggtt ccgggatgcc gaggctaccg
    16261 tcgtatcgat caatgaaatt ggtctataac aaatgcacct tcggtattat tcatactatt
    16321 tagcagaaat acaggttcct ttcatgggag actcaggata tccctccatc ttccatttcg
    16381 atatacaatc accatttgtt tcaaaaatcg aaaaaatttt tttattacgt taaaccttaa
    16441 acaaattgct gcatcaaaca tttaatatga cttgttgatt gacaaggccg ttgtttattt
    16501 attgcatgac acaaaacacc gatagaaaat cattgtgcat gtgtcggata aacccggtat
    16561 tcaatccgaa aatttgatac ctctgtctaa atactggttt caagggattt cttaacagtt
    16621 tggaaacaca gaatcgcatt attattcact ttttttgcag caaaataaaa cgtaacaaat
    16681 cttgagatct gcattattcc tacttatttt tgtgcttgga gcactgaccg cccattctca
    16741 aaattatcgc attttaggcg ttgttaatga ccagattgat tcttcctctc ttatcggagc
    16801 aagtgtcttc cttaagtctt tgcctgattc aaatactgta gccgtagcca ccacggatgc
    16861 cggggggcgg tttgagttca aggatttgaa agccggacct tatgtaatac aatcgaccta
    16921 tgtcgggtat aaaacattta gcaaagcggt ggaactcggg ttcagcgctg ctttcagtat
    16981 tgagatcatg atgaggggag acggcattac cctgggtggc atggaagtca taggccgtac
    17041 acctcctgtc actgtcaagg aagatacctc agagattcgt gccaatcaat ttaaggtcaa
    17101 tcccgatgct aatgctgaag acctcgtaac caagatgccg ggtattcaaa aggtggatgg
    17161 aaagattcag gcacaaggcg aagaggtaaa acgtgtcctg gtagacggtg ccgttttttt
    17221 tggatccgac ccatcggcta cgttgaaaaa ccttcccgct gagatgatcg acaaagtgca
    17281 ggtctttgac aaggcaagtg accaagccca atttacgggt atctctgatg gaaatgagga
    17341 aaaaaccatc aatatcgtga ccaagccgga ttatcgtcag ggcaagttcg gcagagtgtt
    17401 tgcaggatat gctcctgatg acaaatacaa ggc
//
LOCUS       DAOQMC010000016        17143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1189_length_17143_cov_5.016678, whole genome shotgun sequence.
ACCESSION   DAOQMC010000016 DAOQMC010000000
VERSION     DAOQMC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17143
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_17143_cov_5.016678"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PKV31_01320"
     CDS             complement(<1..1344)
                     /locus_tag="PKV31_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB91518.1"
                     /translation="MSKALSVLIALALILQGCTRDQSDFIPTTPIPEYTNDILGVVMG
                     DDDAPIVGAQVRFNGKITLTDIYGIYQFKHVEVSSDHNLLTIEQPGYFSTVRVFGSSG
                     NGRIALKNNLLPKKFEYFFDATNGGSVSEANITFQFPANAFVDKISNQPYEGQVKVAI
                     GYIDPLDPKALDQLPGSVGGLTESESEACLSNYGIIVIEAQGIIGVPLSLATGKTIRA
                     SLRIPGEKLGQASNSTKIWGFDNNLGYWRESGQAILNANNQSYEAQLNDLAFYAFAEH
                     RPAKKLTGRVIDEAGRPLAFASVRINDGQKGFENRAFTDDNGRYNVTVPANTPMIINI
                     NKSKGCTVSLTNDSKINAINENTTLPDLRVKLDGGSYTKISGKLSSCNGNPVHTGYAY
                     AVTEKGSWALLPVANGVFEGYLLNCNDNSNVTIIGVNSESNEYSNEYSFSSGAEIN"
     gene            2025..2519
                     /locus_tag="PKV31_01325"
     CDS             2025..2519
                     /locus_tag="PKV31_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91519.1"
                     /translation="MVREKVKTELIEELAAHHMETSNLPPLASKIFAFLLVNYCRDGY
                     NFDELVDVFKVSKSSVSNSLNLLIQYNFIIQTNRIDERKRRYKITPDYLSIRLNKIIK
                     DLIRERQLSVKLLEFHQTEVNPPGPGEISKAEVYLNHLDTAIRNLSCTLERIDNITNA
                     NQLV"
     gene            2516..3709
                     /locus_tag="PKV31_01330"
     CDS             2516..3709
                     /locus_tag="PKV31_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB91520.1"
                     /translation="MNIRIYGVLSVFTLISFFSCKSDQKQMVMPPPTIPTVEVVNKDV
                     VSYRTFPVSIEGKVNNAVRAKISGYIAQVFVDEGQMVTKGQPLFRLETNVQSQDANAA
                     RSGVSAAQANIEAAKASVSAAQVEVDKLTPLVERNIVSNVQLETAKANLMRAQGQLEQ
                     AKAARKQASSSYSAVAANVNYGIVRSPITGVVGAINFREGSLVGPGDQMPITTVSETS
                     EVYAYFSMNESQYFDFLEQTPGKDVSEKLKNMPEVDLFLPNGRQYAAKGKIKTVTGQI
                     NPQTGTIQFRATFPNKEQILSNGNSGKVQIPVMHKGVLVVPESAAFEQQGATYVYRVG
                     KDTVTNIPIKVIARVHNMVLVESGLKTGDIVVAQGTGKLKPGAAIKPVPTSMDSIVNA
                     IKPIF"
     gene            3712..6867
                     /locus_tag="PKV31_01335"
     CDS             3712..6867
                     /locus_tag="PKV31_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB91521.1"
                     /translation="MIKRLIERPVLSAVISILIVILGVLGLSVLPITQYPDIAPPTVT
                     ISTNYPGANAETVMKSVVIPIEEQVNGVENMIYISSSAANDGSASITVFFEQGTNPDI
                     AAVNVQNRVARATPLLPAEVTRTGVVTQKQQSSALMYLSFYSTNPIYDDIFLQNYLNI
                     NIIPEIKRVKGVGDANAFGGKTYAMRIWLLPDRMAAYGLMPSDVTGAINEQSREAAAG
                     QVGSNSGSSYEYIIRYKGKYDDPKEYEDIVIRALGNGQFLRLKDVARVQLDALSYSGV
                     GEFNGYPAMSMGIFQTPGSNAKEINDDIKTYLNKIEKTLPDGIKYSINFDTNEFLDAS
                     IDKVVQTLLEAFALVFLVVFLFLQDFRSTLIPAIAVPVSIIGSFFFLNLFGFSLNLLT
                     LFALVLAIGIVVDDAIVVVEAVHAKLERGAKNAQAATVEAMSEISGAIISITLVMASV
                     FIPVTFLQGPTGVFYQQFGITLIVAIIISAINALTLSPMLCALFLKGNHEDAEYQQKG
                     LLQKFFYKFDAGFKAVTNRYALLASYLIRRKWIIGVGLLAAIGIIWWSNKTMPKGFVP
                     SEDRGIVFANVELPPGASMERTYNMMKAMDAQARTVPGVVGVTFSTGRSFFSGTGSNN
                     GLAFIRLAPFSQRKDSKDLSVESITKNLYRAVSTIADANIIFFTPPSVPGFGANAGFE
                     IVLLDRGGHEVKDVNSVSRQFIGDLMARPEIQFAQTPFNTNYPQYEMVINVERAGETG
                     VSVASILSVMQAYIGGIYTADFSKYGKQYRVMVQALPEARIDANSLNQLYVRTASGQM
                     TPVAQFVTLERVFGPQSISRYNLYTSVKITGANAPGFSTGDAINAINQVAQSTLPAEY
                     SIDYTGLTREEVNSGSQAMLIFILSLIFVYFLLSAQYESFVQPLSILLSLPFGVMGAF
                     LGQKMAGLENNIFFQIALIMLVGLLAKNAILIVEFALQRRRHGESIVMSGVSAAKARL
                     RPILMTSFAFIFGMMPLVFAVGVGATGNRSIGTGAASGLLIGTIFGVLAVPVFYVIFQ
                     YIQEKIKPIRFDHDNPHETTN"
     gene            6894..8294
                     /locus_tag="PKV31_01340"
     CDS             6894..8294
                     /locus_tag="PKV31_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNB91522.1"
                     /translation="MKGIKYIVWIGLVMTAQSCFVAKKYNRPGDVVKEKYFRTDVLQN
                     DSSTIADISWKQLFTDKQLNQYIGKGLDNNLDIRIALRNIDAAQAYVRQGRAAYFPSV
                     NLGPGYTFSSPSLNSINGQILSERSNINQFDLTAGLSWEADIWGKIRSNERAANATYL
                     QSVAAHQAVKSRLVAAIATTYYQLLALDQQKKVTESTIQNREQSVETIHALKSSGRVT
                     EVAVQQTEAQLYNTRSVIVDIDNSIRLLENAFCILLGENPHPVDRSTLEEQHIDTPLN
                     AGVPAELLANRPDVLSAEYGLINAFELTNIARSNFYPSLRITASGGIQSIDIDKLFSA
                     GSLFGAIAGSLTQPIFNGRQIRTQYEVSIARQEEALIKYKMSVLNASREVSDALYTYK
                     AADEKVKIKQKEFQAYVRAISDSEELMNYGMATYLEVLTARENMFNAQLSIINIEFSR
                     LSAMVELYRAVGGGWR"
     gene            8694..9821
                     /locus_tag="PKV31_01345"
     CDS             8694..9821
                     /locus_tag="PKV31_01345"
                     /inference="COORDINATES: protein motif:HMM:NF037744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91523.1"
                     /translation="MVELSELLIDLIFKQKSATVPELGTFEFVANPARIHFGENIIYP
                     PSQQLVFSDGKMDTGEVGFTEFLVQEKGFDEHEALIRVKAYVHKIKGNLDTLGYSYLP
                     GLGTLHRSENSSLEFTPGDKIKAMKPTLGLPEIKVEPIVREYVDPDKVVRETEVAGPV
                     NDLGDDSRSSKSWVLPVVLAFILVGLSVTGYYLYKMQSDNKGPQEANPVEIPADTAGV
                     EDNQASAVPSADDAGLNGMTDDEGSTYQPDDIVTDSNLSETNTQGISNAETKTETSAS
                     SSSGSTKPVKKVEKKAEKPLEDTPYTGKVCAIIVGVMSKPENARRLARTITNAGFQPF
                     AYTSKGLTRVGATCNCDDQSIDSTLRQMKKISADAWLYESE"
     gene            complement(9831..10421)
                     /locus_tag="PKV31_01350"
     CDS             complement(9831..10421)
                     /locus_tag="PKV31_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB91524.1"
                     /translation="MNHELLVNNIRRKIDLSEKEQAYILELFTEKKLKRKQFILHEGQ
                     ANHHLSFVVNGCLRAYSVDKNGFEHILQFAPNDWWITDIHSFVTGMPASLNIDAIEES
                     TILMITREDREKAFSLVPKLERYFRILMEKNLAANRQRIIDRLSLTAKERFVSFCNTY
                     PELINRIPQKQMASYIGVTPEFLSKMRSEYLRSLKH"
     gene            10866..11231
                     /locus_tag="PKV31_01355"
     CDS             10866..11231
                     /locus_tag="PKV31_01355"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNB91525.1"
                     /translation="MATNILGQAFEPATPPTSDEKTMALLSHLLVLVASFVAPLVIYL
                     LKKDESSFVANNAKESLNFQITIAIAIGVSVLLMMVLIGFLLIFAVSLANLVYVIIAT
                     MRASEGKEYQYPFCIRLIK"
     gene            11367..11924
                     /locus_tag="PKV31_01360"
     CDS             11367..11924
                     /locus_tag="PKV31_01360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNB91526.1"
                     /translation="MLNLKIIHASTREGRRGLPVSKWVVDAARKETDFEVEFIDLKEL
                     GLPFIDEPEHPRLKQYVHRHTVEWSNKIDHADAFIFVTCEYNHGMPATLKNALDFLVQ
                     EWAFKPVAFVGYGGISAGTRAIQQLKQVTYSLKMFPFDGVLLPFFNTQISEEGVFLPT
                     SSNSRALDMMFKELKRLGEGMKALR"
     gene            12033..13139
                     /locus_tag="PKV31_01365"
     CDS             12033..13139
                     /locus_tag="PKV31_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB91527.1"
                     /translation="MSKYFSTWMMAIIALFSFFTNELIAQNSKKTENVVLILIDGYRW
                     QELFRGAERGLLADKRFNSEDSIARFKKYWDEDPLVRRKKLMPFTWEYIARHGQIYGN
                     RDYDNLVEVTNPYWISYPGRAELYSGFVDSSIQKNSHPNNSNINVLEFIHRQKGFEGS
                     VAAFVCWRPAGLALNKENAGFLINVPWEDVKGEKLTDAQKLANELQHYAPLVFGYGER
                     LDFAVYALASTYIMANHPRFTYIDLGDTDEYAHAGQYDKYLDDIFNIDRMIGNLWNSM
                     QADAFYKDKTTFIIVTDHGRGEGDQWTDHYHTVPHSGETWAMILGPDSKALGEVKTPG
                     RIYQNQFAATMARLLNLEYTANGRAGMPVESMMR"
     gene            complement(13300..13707)
                     /locus_tag="PKV31_01370"
     CDS             complement(13300..13707)
                     /locus_tag="PKV31_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNB91528.1"
                     /translation="MPGLDKTRVDKWLWAIRFFKSRSIASDACTGGKVKINGISAKPS
                     GLVKAGDIIECKKDHINYRLRVITVIDKRVGAPIAVTCYENITPEEELNKFESWFAGK
                     ARPEQRDKGTGRPTKKERRDIDDYKDSIFFEED"
     gene            complement(13707..14483)
                     /locus_tag="PKV31_01375"
     CDS             complement(13707..14483)
                     /locus_tag="PKV31_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HNB91529.1"
                     /translation="MQKEIKLSKISTKAPKSMKKEDLITPTMELYERIGHLQHILYAE
                     KKHSVLIVFQGPDASGKDGATKNVFSRCSPQGVRVEAFKKPSEEEMNHDFLWRIHANT
                     PEKGFIQVFNRSHYEDILIQRVHKWIDEDRVHKRMAAINAFEELLQFDNNTLVLKFFM
                     HISKDNQLKQLKERLEEPEKNWKHNPNDWEERKLWNEYTKAYEYALNHSSIPWHIVPV
                     DNRWYRNYFIASVIVRELEALNMKLPVLDKNTLLADNSDD"
     gene            14705..16063
                     /locus_tag="PKV31_01380"
     CDS             14705..16063
                     /locus_tag="PKV31_01380"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNB91530.1"
                     /translation="MKFALPCVKNNLHFFLILALWVVSVKVSEAQSDFRIHRCGNTLM
                     DKIMEERHPGFLDNQNKAFSTAQNMPMPRNGDVLRIPVVFHVIYNTDKQNIPDEYIYN
                     QMEVINDAYRNRHGDTANTRPVFKHLAADAEIEFFLADIDPDGNPTNGITRTQTDIET
                     FGDLTLILGGFNLEHFEKMKHSNQGGHDAWPTNRYLNIWVADAGIKFLGLYFPALLGL
                     ATPPRYPTLPDNWPDEDFGDIVDGVFLQYQTIGNNNPYKSDLSDLVSAGRTAVHEIGH
                     YLGLRHINGDEECGSDGIDDTPVMDLSTQMANACPGTDVNTCGAGEPGDLPDMWENYM
                     DYSNDICQTLFTIGQVGHMRKVLTGQRDTLLNWALNTQRTNIEAMNLYPNPANSAIQI
                     GSIPLGGNMIISNSLGKVVMHFERLQEYRIDVSALPPGCYFITYLSEQTKKYNSRFVI
                     VR"
     gene            16304..16837
                     /locus_tag="PKV31_01385"
     CDS             16304..16837
                     /locus_tag="PKV31_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNB91531.1"
                     /translation="MGDFMSQPMVQIVLRCIAVYLFVVLALRIFGKTELAQLSVVDLV
                     FILLISNSVQNAMVGPDTSLGGGLVAAFSLFTLNYGFKRLIAKNKKVSDLIQGKEILL
                     AYNGKVHMANLKKAGLTESELNAAIREHGVQDISGVDLAMLEVDGNISVISDDYKKRA
                     SLPSPHKHKHKWKGGIN"
     gene            complement(16900..>17143)
                     /locus_tag="PKV31_01390"
     CDS             complement(16900..>17143)
                     /locus_tag="PKV31_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNB91532.1"
                     /translation="NDLVINIVKAKQLTNFRASGKDDAARVTPPRKFSLEEAMEYIQA
                     DEYLEVTPKSQRLRKIVLSESERRRLANPALAGMKM"
BASE COUNT         4841 a         3585 c         3832 g         4885 t
ORIGIN      
        1 atttatttcc gcaccggagg aaaaggaata ttcattgcta tattcattac tttcactgtt
       61 tacgccaata atagtgacgt tactgttgtc attacaattg agcaaatatc cttcaaagac
      121 accatttgcc acaggcagta aagcccatga acctttttca gtcactgcat aggcatagcc
      181 tgtatggacc gggttgccgt tgcacgatga taatttaccg cttatcttcg tatagctacc
      241 cccatcaagc ttaaccctta gatcaggtaa ggtggtgttt tcattaatgg cattaatctt
      301 ggaatcattg gtcaggctta ctgtgcagcc tttactttta ttgatgttga taatcatggg
      361 tgtattggcg ggaacggtga cattatatcg cccattatca tcggtaaatg cccggttttc
      421 gaatcctttc tgcccatcat tgatgcgaac tgaagcaaag gcaagaggcc ttccggcttc
      481 gtcgatcacc cttccggtca gttttttggc cggccgatgt tcagcaaatg cataaaaggc
      541 caaatcattc aattgagctt cataactttg gttgttggca ttgagaatag cttgtccact
      601 ttcgcgccaa taaccgagat tgttatcgaa gccccaaatc ttagtggagt tggaagcttg
      661 accaagtttt tctccaggta ttcgaaggct tgcccttata gtttttccgg tagccagact
      721 aagcggaaca ccaatgattc cttgcgcttc aatcactata ataccataat tgctaagaca
      781 agcctccgat tctgattcgg ttaagccacc gacagaaccc ggcaattggt ccaaggcctt
      841 tgggtcaagg ggatcgatgt agcctattgc cactttgacc tggccttcgt aaggttgatt
      901 ggaaattttg tccacgaatg cattcgcagg aaattggaag gtaatgttag cctccgatac
      961 tgagcccccg ttagttgcgt caaagaaata ttcaaatttc ttcggcaaca aattgttttt
     1021 cagcgcaatc ctaccattgc ctgacgaacc gaatacccta acggtactga aatacccggg
     1081 ctgttcgatg gtcagaagat tatggtcaga actcacctca acatgcttaa actgatagat
     1141 cccgtaaata tccgtcaatg taattttacc attgaaccgt acctgagcac ccacgattgg
     1201 ggcgtcgtca tctcccatca caacacccag aatatcattg gtatattccg gaattggtgt
     1261 agtggggata aaatcacttt gatcccgggt gcatccttgc aaaatcaatg caagggcaat
     1321 gagaacagat aatgccttac tcatcatttc agtttaatat cctaattacc tttaatcaac
     1381 ccctttcgtt gtccgaaaat aaatttttgt tagatttttt tataaagata ctataaaagt
     1441 ctgagttgca aggcatttat aagtcctgca aggcaggtgt ggaggatgga tttgttcaat
     1501 gttctatcgg tgtgttcttc caatacctga tgtgttttca gatgttagat gattcgcgtc
     1561 tgtgcttttt tgataaaatt ataaaataac ataaaagtgg ctataaataa cataaaataa
     1621 ataccataga acataaagcg gtgttttgct gctaagtttt ctgttcatac aatccgtgga
     1681 caagggtaga atgatgactt tccccccggt ttcatgtaac aaaatttgat aggaaggcag
     1741 gcacaagaat agcccggtct gtttattgga attgttcgtc caattggtat taaatgttga
     1801 ggtatgtcag gtatgttatc tctaaagcgt ttttgaaatg gacaacacag gatatctatc
     1861 aatcgcaata aacttggctc atcaacagcc cttaagggca gaaggctgaa aataaatgac
     1921 ctgtttttct taaaaacttt gactttttgc aaacatttta tcactttgat tcgtttcgtt
     1981 ccgttgttac cgaacaaaca aaagttaaga aataaatatc atctatggtg agagaaaaag
     2041 tgaaaaccga attgattgaa gaattggctg ctcatcacat ggagacctct aatctgccac
     2101 cgttggcatc gaagatattt gcattcctgt tggttaatta ctgcagggat ggttataatt
     2161 ttgatgagtt ggttgatgtg ttcaaggtaa gcaagagttc tgtttccaat agtctcaatc
     2221 ttctgattca atataacttc atcattcaaa ccaatagaat cgatgagcgt aagcggcggt
     2281 ataaaatcac tccggattat ctttccatcc gattgaataa aatcataaaa gacttgatac
     2341 gcgaaagaca gttgtcggtc aaattgttgg aatttcatca aacagaggtt aatccacccg
     2401 gtcctggcga aatctccaaa gcagaagtct atctcaatca tctcgacaca gctatccgca
     2461 atctgagttg tacccttgaa agaatagata atataacaaa tgcaaatcaa ttagtatgaa
     2521 tataagaata tacggcgttt tgtcggtttt tacccttatc tcctttttct cctgcaaaag
     2581 cgatcaaaaa cagatggtga tgcctcctcc gacgattccc accgtcgagg ttgtcaacaa
     2641 ggatgttgtc tcatacagga catttccggt aagtatagag ggcaaggtca acaatgctgt
     2701 cagagcaaag atctcgggtt atatcgccca ggtctttgtc gatgaagggc agatggtaac
     2761 caaaggtcag ccgttattca ggctggagac aaatgtacaa tcgcaggatg ccaacgctgc
     2821 acgttccggt gtaagtgctg cacaggcaaa tatcgaagct gccaaggcct cagtttcggc
     2881 tgcccaggtg gaagtggaca aactgacacc tcttgtggaa agaaatattg tcagtaatgt
     2941 acagttggaa accgcaaaag caaatcttat gcgtgctcag ggccaactgg aacaggcaaa
     3001 agctgcacgg aagcaagcct cgtcttccta tagcgccgtt gcagccaatg ttaattatgg
     3061 cattgtaagg agcccgatta ccggtgttgt cggcgccatc aattttagag aaggcagtct
     3121 ggttggaccg ggcgatcaga tgccgatcac cacagtgtca gagacgagcg aagtgtatgc
     3181 ctatttttcc atgaatgaaa gccagtattt tgattttctg gagcagacgc cggggaagga
     3241 tgtctcagag aaattgaaaa acatgccaga agtggatctc ttcctcccca atggccggca
     3301 atacgccgca aaagggaaga taaagaccgt caccggccag atcaatccac agacaggtac
     3361 tatccagttc agggcgactt ttcctaataa agaacaaata ttgagtaatg gcaatagcgg
     3421 taaagttcag attccggtga tgcataaagg tgtgttggtc gtgccggaat ccgcagcttt
     3481 cgaacaacag ggcgctacct acgtatatcg ggtaggcaag gatacagtaa caaatatacc
     3541 gatcaaggtc attgccagag ttcataatat ggtgttggtg gaatcaggac tcaagactgg
     3601 agacattgtt gtagctcagg gtacaggaaa actcaagcct ggtgcggcca tcaaaccggt
     3661 accaacatcc atggacagca ttgttaatgc cattaaaccc atattttaat catgataaag
     3721 cggttaattg aaaggccggt cctgtcggcc gtcatctcca ttttgatcgt cattctgggt
     3781 gttcttggcc tcagtgttct ccctataact cagtaccccg atattgcacc tccaacggtc
     3841 actatctcca ccaactaccc gggagccaat gccgagaccg ttatgaagag tgtggttatt
     3901 ccaatagagg agcaggtcaa cggggtggaa aatatgatct acatttcctc atcggcagcc
     3961 aatgatggat cggcgagcat cactgtcttc tttgagcagg gcactaatcc cgacatcgca
     4021 gccgttaacg tccaaaatcg tgtggccagg gcgacacctc ttcttcctgc cgaagtgaca
     4081 agaacagggg ttgtgactca gaagcaacag agttctgcat tgatgtatct gtctttctat
     4141 tctaccaacc cgatctatga tgatattttc cttcaaaact acctgaatat caatattatt
     4201 ccagagataa aaagggtcaa aggtgtcgga gatgccaatg ctttcggtgg aaaaacttac
     4261 gccatgcgaa tctggctttt gcctgatagg atggctgctt acgggttgat gccttccgat
     4321 gtaaccgggg caatcaatga gcaaagccgt gaggccgcag caggacaggt aggaagcaac
     4381 tcgggcagtt cctacgaata tatcattcgt tataagggca agtacgatga tccgaaggaa
     4441 tatgaagata tcgtgatccg tgcattgggt aacggacaat ttcttcgcct gaaggatgtt
     4501 gccagggtac agctggatgc tttaagttat tcaggcgtcg gagaattcaa tggttatccg
     4561 gcgatgagta tgggtatctt ccagacgccg ggctctaatg caaaggagat caacgacgac
     4621 atcaaaacgt acttgaataa aatagaaaaa acgctgcctg acggtattaa atattcgatc
     4681 aactttgaca cgaatgaatt tttagatgcc tccatcgata aggttgtcca aacattgctt
     4741 gaagcatttg cactggtctt tctggtggta ttcctgtttc tccaggactt cagatccacc
     4801 ctgattccgg caattgcagt accggtgtcc atcatcggta gttttttctt tcttaatctg
     4861 tttggttttt ctttaaacct gctgacctta tttgccctgg tgctggccat tggtatcgtg
     4921 gtggatgatg ccattgtggt ggtggaagcc gtacatgcca agttggaaag gggagccaaa
     4981 aacgcgcagg cagctactgt tgaggcgatg agtgaaatct ccggagccat catctctatc
     5041 actcttgtca tggcatccgt ctttattccg gtgactttcc tccagggtcc taccggagtg
     5101 ttctatcaac agtttggcat taccctcatt gttgccatca tcatttcggc tatcaacgcc
     5161 cttacactca gcccgatgct ttgcgctctc ttcttaaaag gaaatcatga agacgcggaa
     5221 tatcagcaaa aaggcttatt gcaaaagttc ttctacaaat ttgacgcagg attcaaggca
     5281 gtcactaaca ggtatgcgct gctcgcttca tatttaataa gaagaaaatg gatcatcggc
     5341 gttggattat tggccgccat aggcattatc tggtggtcaa acaaaaccat gccgaaaggt
     5401 tttgtgccaa gcgaagaccg aggcatcgtg tttgccaatg tggaattacc tcccggagct
     5461 tctatggagc gtacttacaa catgatgaag gcaatggacg ctcaggcaag aacagtgccg
     5521 ggtgttgtgg gggtaacctt ctctacaggg cgcagtttct tttccggcac aggcagcaac
     5581 aacggtctgg cctttattcg acttgccccg ttcagtcaaa gaaaggatag taaggatctt
     5641 tcagtggaga gtattacaaa aaatctttat cgggcggtct cgaccattgc cgatgccaat
     5701 atcatctttt tcacgccacc gagtgtaccg ggcttcggtg ccaatgccgg atttgaaatt
     5761 gtcttgttgg atcgtggagg gcatgaagtc aaggacgtca atagtgtttc acgtcagttt
     5821 attggcgatt taatggcaag acctgaaata cagtttgctc agacaccttt caataccaac
     5881 tacccacagt atgaaatggt cataaatgtg gaaagagctg gtgaaacagg cgtttccgtt
     5941 gccagcattc tttctgtcat gcaggcttat attggcggta tctatacagc cgacttctcc
     6001 aaatacggaa agcaataccg tgttatggtt caggcattgc cggaagcccg gatcgatgct
     6061 aacagcctta atcaattgta tgtacgtact gcatccgggc agatgacacc tgttgctcaa
     6121 ttcgtaacgc tggaaagggt attcggcccc cagtccattt cgcgctacaa cctatacact
     6181 tccgttaaga ttaccggagc caatgctccc ggcttcagta ccggtgatgc catcaatgcc
     6241 atcaatcagg tggctcaatc gacattgccg gctgaataca gcatagatta tacgggtctg
     6301 acccgggaag aagtgaattc cggctcccag gcaatgctga tttttatcct tagtctcatc
     6361 tttgtgtatt tcctgctttc agcgcagtat gaaagcttcg tccagccact ttccatcctg
     6421 ctctccctcc cctttggagt catgggagct tttctcggtc aaaagatggc aggattggaa
     6481 aacaatattt tcttccagat agcattaatc atgctggttg gcctcctcgc caagaatgcc
     6541 atattgatcg tggagtttgc actccaacgt cgcagacatg gagaatctat tgtcatgtcg
     6601 ggtgtaagtg cagctaaggc caggttacgt ccgatcctga tgacttcctt tgcatttatc
     6661 tttggtatga tgcctctggt atttgcagtt ggcgtaggtg cgacaggcaa tcgatccata
     6721 ggtacaggcg ctgcatccgg cttattgata ggtaccatat ttggcgttct tgcagttccg
     6781 gtgttctatg tgattttcca gtatatccag gaaaaaatca agccgattcg gtttgatcat
     6841 gacaatccgc atgaaacaac aaattaaatc caattcaaaa aggaacaaga taaatgaagg
     6901 gaatcaagta tattgtatgg attgggctgg taatgacagc acaatcctgt tttgtggcca
     6961 aaaaatacaa ccggccgggt gatgtagtca aggaaaaata cttccgcacg gatgttctgc
     7021 aaaacgatag ctcgaccata gccgatatct cctggaagca gctttttaca gacaagcagc
     7081 tcaatcaata tattggaaaa ggactggaca acaacctgga tatcaggatt gctctccgaa
     7141 acatcgatgc ggctcaagct tatgtcagac aggggcgcgc tgcatatttt ccaagtgtca
     7201 atctgggacc tggctacaca ttttcatcac cgtccctgaa ttctatcaat ggtcaaatcc
     7261 tttcagaaag atccaatatc aaccagtttg atctgactgc cggattgtct tgggaggcag
     7321 atatctgggg taagatcagg agtaatgagc gagccgccaa tgccacctat cttcaatcgg
     7381 ttgcagcaca tcaggctgtc aaatccaggc ttgtggcggc gattgcaacg acttattatc
     7441 aactgctggc gctcgatcag caaaaaaagg tgacagaatc aacgattcaa aacagagaac
     7501 agagcgtaga gactatccat gccctgaaat cttcaggtag ggttactgaa gtggcggtac
     7561 aacagacaga agcacagtta tacaatacac gttcagtcat tgttgatatt gataacagta
     7621 ttagattgct tgaaaatgct ttttgtatcc ttctgggaga gaatccacat ccggtggatc
     7681 ggtctacgct ggaagaacaa catatagata caccgctcaa tgccggagtg cccgcggaat
     7741 tgcttgccaa tagaccggat gttttgagtg cagaatacgg actcatcaat gcttttgaac
     7801 taaccaatat agccagaagt aatttctatc cgtcactccg catcacagcg agcggaggca
     7861 ttcaaagtat cgatatagac aaattgttta gtgccgggtc cctcttcgga gccatcgcag
     7921 gctctcttac acaaccaata tttaatggga gacaaatacg cacacaatat gaagttagca
     7981 tagcgcgaca ggaagaagcg ttgattaaat acaaaatgtc tgtgttgaat gcaagcaggg
     8041 aagtctccga tgccttgtac acttacaaag ctgctgatga gaaggtaaag atcaaacaaa
     8101 aggaatttca ggcctatgtc cgggcaatta gtgattcaga ggaattgatg aattacggaa
     8161 tggcaaccta tctggaagtg cttaccgccc gtgaaaatat gtttaatgca caattgagta
     8221 tcatcaacat tgaattcagc agattgagcg cgatggttga gttatataga gctgtcggcg
     8281 gaggatggag atgatcggtg actgttaaat gttgttggat tcatgtaatg gggtttgaag
     8341 aggagtgcta accttaaatt ttccaggtag gtagatgatt aataatcgtc tattaccttt
     8401 tatcgcaccc tgacaacatt agatcctgaa attcacaggc tatttgcagt ttgttttgac
     8461 gtgatggccg taaaagccat aaatactata gcgtcaaatt attattttta tggctacttg
     8521 aatttttatt tcaattcaac ttatccaagg gcgttttccc cttacatttt cattatttat
     8581 ccttgaaatc gatcgccgga ccgttagttg tgaaaataat tatatatatt cttttgtatt
     8641 tcatagcaag ttgtgaagaa tgtatttatc tttgtagtta aatatctaca agagtggtcg
     8701 agttaagtga actattgata gatttgattt tcaagcaaaa atcagcaacc gtgcctgaat
     8761 tgggtacgtt tgagtttgtt gcaaacccag ccagaataca ctttggggaa aatattattt
     8821 atccgcccag tcaacaattg gttttttccg atggaaaaat ggataccgga gaagttggat
     8881 ttaccgaatt tctggttcag gagaaaggct ttgatgaaca tgaagctctt atcagggtca
     8941 aggcctatgt tcacaaaatt aaagggaacc ttgacactct gggatattct tatcttcccg
     9001 gcctcggcac gcttcaccga tccgaaaatt cttcacttga atttacaccc ggtgataaga
     9061 ttaaggctat gaagcctacc cttgggcttc ctgaaatcaa ggtggagcca attgttcggg
     9121 aatatgtcga tcctgataag gtggtcaggg aaacagaggt ggctggtccg gtgaatgatt
     9181 taggcgatga cagcagatca tcgaagtctt gggttctccc ggtagtgctt gcctttattc
     9241 tggtagggct tagcgtaacc ggttattacc tgtataaaat gcaatcagac aataaggggc
     9301 ctcaagaggc aaatccggtt gaaataccag cagatacagc aggtgttgaa gataatcagg
     9361 caagtgccgt accgtccgct gatgatgccg gtctgaatgg aatgaccgat gatgagggtt
     9421 caacctatca accggatgat attgttactg acagtaatct ttcggaaacc aatacgcagg
     9481 gaatttcaaa tgcggaaaca aaaactgaaa catctgcttc gtcatcctca ggtagtacaa
     9541 agccggttaa aaaggtggaa aagaaggctg aaaaaccttt ggaagataca ccctatacag
     9601 gaaaagtctg tgccatcata gtaggagtca tgtcaaagcc ggaaaatgcc agaagattgg
     9661 ccaggaccat aacgaacgca ggttttcagc catttgccta tacttcaaag ggtttgacac
     9721 gagtgggggc tacttgcaat tgcgatgatc aaagtattga ttcaacgctc aggcaaatga
     9781 agaaaatcag cgctgatgcc tggctgtatg aatcagaata ggcaaggcct tcaatgcttc
     9841 agtgatcgaa ggtactcact tctcatttta cttaaaaatt ccggtgttac accaatgtag
     9901 gaagccatct gcttttgtgg gatcctgttg atcagttcag gatatgtatt gcagaaactg
     9961 acaaatcttt cctttgctgt caggcttagc cggtcaataa tacgttgcct gttggctgcg
    10021 agattcttct ccataagtat cctgaaataa cgttccaact taggaacaag ggaaaaggcc
    10081 ttttcacggt cttcccgggt tatcatcaga atggtacttt cttcaatagc atcaatgttt
    10141 agagaggccg gcattccggt aacaaaactg tgaatgtcgg taatccacca atcatttgga
    10201 gcaaattgca ggatgtgctc aaatccgttt ttgtcgacag aataggcgcg aaggcaacca
    10261 ttcactacaa atgataaatg gtgattggcc tggccttcat gcaagatgaa ttgcttcctt
    10321 ttaagcttct tttcagtaaa tagttctaaa atataagctt gctccttttc tgaaaggtcg
    10381 atttttcgcc ggatattatt tacaagtaat tcatgattca ttctgtaaaa ttcaataaaa
    10441 attctcaaaa aagcaatatt ggttgcataa atgaccgggg aagcttttaa tttcttttaa
    10501 ttcagccaaa cgcaccttgt aatgtttaat ggctccggaa caatacaaaa ttagtaggag
    10561 gttataagct attcgcccgc ttttccttcc aatcaatccg gctttctaat tggtttgaca
    10621 agtatccata tcaaggatac catgcgcgct atacttatgc gatcgggact atggagaagt
    10681 tggaggacgg acatttgtat ttatattttg gtgccaaacg attttgtcct aatccggacc
    10741 aaacgtgcat tcaccccgca tgaatcagca attttgaaat aaaatgcact gaatatttat
    10801 aaaaacaagc gggtttttca tagaaaatcg tgaagtttag taattttaca gtaaaaccaa
    10861 taattatggc cacaaacatt ttgggtcagg ccttcgaacc ggccactccg cccacctccg
    10921 acgaaaagac aatggcattg ttatctcatt tgctcgtttt agtagcctcc tttgtcgccc
    10981 ctctggtaat ttatctatta aagaaagacg aatcttcctt tgtagccaat aacgccaaag
    11041 aatcattgaa tttccagatt actattgcca ttgctatagg cgtgagtgtc cttttgatga
    11101 tggtcctgat cgggtttctt ttgattttcg cagtttcatt agctaatctc gtatatgtta
    11161 tcatagcaac catgagagct tcggaaggga aagagtatca gtatcctttc tgcattcgac
    11221 ttatcaaata aacattcatt acttgaatct tttttcacaa tggctgactt actccggtca
    11281 acccaatgac tgttttatgt aatggttaga gacatctgta taccggaact gaatattttg
    11341 tacaaaacgg aaaaagcaat aaggatatgt tgaatttgaa gataatccac gccagtacaa
    11401 gagaagggcg ccgcgggttg ccggtctcga aatgggtggt ggatgcggca agaaaggaaa
    11461 cagattttga agttgaattc atcgatctga aggaacttgg acttcccttc attgacgagc
    11521 ctgagcatcc tcgactcaag caatacgtgc atcggcacac agtggaatgg agtaataaaa
    11581 tcgaccatgc cgatgccttc atctttgtga catgtgaata caatcacggt atgccggcca
    11641 cacttaagaa tgcactcgat tttttggttc aggaatgggc ctttaagccg gttgcctttg
    11701 tcggatatgg cggtatatct gcagggacac gtgctatcca gcaattgaag caggtcacat
    11761 attcacttaa gatgtttccc ttcgatgggg tgttgttgcc ttttttcaat acacagatca
    11821 gcgaggaggg tgtttttttg ccgacctctt ctaacagtag agcgcttgat atgatgttca
    11881 aggaactaaa acgtcttgga gaagggatga aggcgttgcg ttagattatc cggagaaaac
    11941 ccttgcgttg tttgaaaatt gaccttatat ttgaatgggt tgtaattagg taacaacgaa
    12001 ttttattgaa tgataaaaat tataatgtca agatgagtaa atatttttct acatggatga
    12061 tggctatcat cgcacttttc tcttttttta caaatgaact catcgcccag aatagcaaaa
    12121 agacagaaaa tgtagtgttg atcctgatcg atggatatcg ttggcaggaa ttgtttagag
    12181 gggcagagag gggtctgttg gcggataaga gatttaacag cgaggattct atcgcccgat
    12241 ttaagaaata ctgggacgag gatccgttgg tgcgcagaaa aaaattgatg ccctttactt
    12301 gggaatacat cgccaggcat ggccagatat atggcaaccg ggactatgac aatttggttg
    12361 aagtgactaa cccatactgg atctcctatc cgggtcgcgc tgaattatac agtggttttg
    12421 tcgattcttc catccagaag aacagccatc ccaataattc aaatatcaat gtactggaat
    12481 tcatccatcg gcaaaaaggc tttgagggaa gtgtagcagc ctttgtatgc tggcgtccgg
    12541 ccggcctggc cctgaataag gagaatgccg gttttctcat caatgtgccg tgggaggatg
    12601 tcaagggcga aaagctgact gatgcccaaa agctggccaa tgaattacag cattatgcac
    12661 cactcgtttt cggctatggc gaacgactgg attttgccgt ttatgcactg gcttccacct
    12721 atatcatggc caaccatcca cgatttacct atatcgatct gggtgataca gacgaatatg
    12781 cccatgcagg acagtatgac aaatatctgg atgacatatt caatatcgac agaatgattg
    12841 gcaatctatg gaattccatg caggctgatg ctttctataa agataaaacc actttcatca
    12901 tcgtcactga ccacggccgc ggtgaaggcg accagtggac cgatcattat catacggttc
    12961 cgcattccgg tgaaacctgg gcaatgatcc tcggacctga cagcaaagca ttaggggagg
    13021 taaagactcc cggcaggata taccagaatc agtttgcggc aactatggca aggcttctca
    13081 atctcgaata tacagcaaat ggcagggccg gaatgccggt ggaaagtatg atgaggtagc
    13141 gtatttacta cttttggact actaaaaatg aaccataaaa ggtaaaagaa atccgaataa
    13201 tctttttgtg ccctacgtgt atttgtatcg gaaggaaatt cccgaattca atatataaaa
    13261 cacacgggtc tattaatcag gtaaataatt aacggtaatt taatcttctt caaagaagat
    13321 tgaatcctta taatcgtcta tatcgcgacg ttctttcttt gtaggacgtc ctgttccttt
    13381 gtccctttgt tccggtctgg ccttaccggc aaaccagctt tcaaacttgt tgagctcttc
    13441 ttcaggtgtt atattctcgt aacaggtaac ggcaattggg gcgccaacac gcttgtcaat
    13501 gacggtaatc acccgaaggc ggtagttgat atggtctttc ttacactcta tgatgtctcc
    13561 tgctttaaca agcccggatg gtttagcact tatgccattg atcttcactt tgccaccggt
    13621 acaagcatct gaggctatgc tgcggctttt aaaaaaccga atcgcccaca gccatttgtc
    13681 tacccgcgtc ttatcaagcc ccggcatcaa tcatcagaat tatctgccaa taaagtgttt
    13741 ttatcaagta caggtaattt catgttgaga gcttcaagtt cacgaactat cacagaggca
    13801 ataaaataat tgcgatacca tctgttatcg acaggcacga tatgccatgg aatggagcta
    13861 tgatttaaag cgtattcata ggctttggtg tattcattcc atagcttccg ctcctcccag
    13921 tcattgggat tgtgtttcca gtttttttcc ggctcctcaa gcctttcctt aagctgtttg
    13981 agttgattgt ctttactgat gtgcatgaag aacttaagga ccagtgtatt gttgtcaaat
    14041 tggagtaatt cttcaaatgc attgatggca gccatgcgct tatgtacccg atcctcatca
    14101 atccatttat gtacccgctg aatcagaata tcttcataat gagagcggtt gaaaacctga
    14161 ataaatccct tttccggtgt attggcatga atcctccaaa gaaaatcatg attcatttcc
    14221 tcttcactcg gtttcttaaa cgcttctact cgcacaccct gaggactgca tcgggaaaac
    14281 acattcttgg tcgcaccatc tttcccgctg gcgtcaggtc cttgaaaaac aatgagaacg
    14341 gagtgtttct tctcggcata taaaatatgt tgcaaatgac caatgcgctc gtatagttcc
    14401 attgtcgggg taatgagatc ttcctttttc atggatttgg gtgcttttgt agatatttta
    14461 gagagtttga tttccttctg catttgaata ctttgattta agtaaaataa aagacaatca
    14521 tcagtcaaat atagctatct gtgaaaatac ttcataattc agcgtcagaa attatccatt
    14581 gcctgttgca gatccgccag ccagatcctt cttctcctca tttttgtctt caaaataaat
    14641 tattgggaat ggtataaatc aactaatttg gagccaatta ttacttttaa aaaatattat
    14701 actcatgaaa tttgccttac cctgtgtgaa aaataatttg cacttttttc tgattcttgc
    14761 cctgtgggtt gtttcggtca aagtgtctga agcccagagt gatttcagga tccaccgatg
    14821 cggaaatacg ctgatggaca aaattatgga agagaggcat cccggtttcc tggataatca
    14881 aaataaagct ttcagcactg ctcaaaatat gccgatgcct agaaatggcg atgttctgcg
    14941 aattccggtt gtgtttcacg tcatctataa tactgataag caaaatatac cggatgaata
    15001 tatttacaat caaatggaag ttatcaatga tgcttatcgc aacagacatg gcgatactgc
    15061 gaatacaaga ccggtattca aacatttggc ggctgatgct gagatagagt tttttctggc
    15121 cgatatcgac ccggatggca atccaaccaa cggcattacc aggacccaaa cagatattga
    15181 gacatttggt gacctcaccc ttatcctggg cggtttcaat ctggaacatt ttgaaaaaat
    15241 gaagcattct aatcagggtg ggcatgacgc ctggcctact aatcgctatc ttaacatatg
    15301 ggtggctgac gcaggtatca agtttttggg tttgtacttt ccggcattgc taggactggc
    15361 aaccccgcca agatatccca cactgccgga caactggccc gatgaagatt tcggtgatat
    15421 cgtcgatgga gtgttcctgc aatatcaaac aatcggcaat aacaatccat acaaatcgga
    15481 cctttccgat ctggtgtctg caggacgtac tgcagtacat gaaataggcc actatcttgg
    15541 tttacgtcat atcaatggcg atgaagaatg cggttcagac ggaattgatg atacgcctgt
    15601 catggacctt tccacacaaa tggctaatgc ttgccccggt actgatgtaa atacctgtgg
    15661 tgcaggagag ccgggggatt taccggatat gtgggaaaat tacatggatt attccaacga
    15721 catttgtcag actctattca caatcggaca ggtcggccat atgagaaaag tgttgaccgg
    15781 gcaacgcgat acactcctca attgggcatt gaatacacaa aggacaaata ttgaagccat
    15841 gaacctgtat cccaatcccg ccaacagtgc gattcaaatt ggcagtattc cacttggtgg
    15901 aaatatgatt atcagcaatt ccttgggaaa agtggtcatg cactttgagc ggttacagga
    15961 atacaggatc gatgtatcag cccttcctcc gggatgttat ttcataacat atttgtctga
    16021 acagactaag aaatacaaca gccgattcgt tatagtgagg taatgatagt acattaacgg
    16081 atatgacagg tgtttggtat tattgagctt taaagctggg accaatctac cttccggcca
    16141 atactaattt tatttttaac cttgtccgcg ggaatgtttt ttcattgatt ggatattaag
    16201 attaattacc tttctggtat gatttccgga ataataatac tggtcatttt atgttaactt
    16261 ttttcggaac aatgattatt ttgaggtaaa aattcaggga attatgggtg attttatgtc
    16321 acaaccgatg gtgcaaattg tattgagatg tatagcggta tatctgtttg ttgtgttggc
    16381 actgcgcata ttcgggaaga cagaattggc ccaactttct gttgttgacc tcgtattcat
    16441 cctgcttatc agtaacagtg tgcagaatgc gatggttgga ccggacacaa gcctgggcgg
    16501 tggtttggtg gcagcctttt ctctcttcac gctcaattat ggattcaaga gactcatcgc
    16561 aaaaaataaa aaggtcagtg atttgattca gggaaaggag atacttctgg cttacaacgg
    16621 caaggtgcat atggcaaacc taaaaaaagc cggtctgact gaatccgagt taaatgcagc
    16681 tatacgggaa catggcgtac aggacatctc cggggtagac cttgcgatgc tggaagtaga
    16741 tggaaatatt agtgttattt cggatgatta taaaaaaagg gcttcccttc cttcacctca
    16801 taagcacaag cataaatgga agggcgggat caattgatta ctgcccggcc ccccaattta
    16861 ttttaatggg tcgaatgtta ttacaaataa ttgtcaggtt tacatcttca tgccggccaa
    16921 tgcgggattg gccaaacggc ggcgttcact ctcagaaaga actatttttc tgagtcgctg
    16981 acttttaggt gttacctcca gatattcatc agcctggata tattccatag cctcttccag
    17041 ggagaattta cggggtgggg tcaccctggc tgcatcgtcc ttaccggaag ccctgaagtt
    17101 ggtcaactgc ttggctttca cgatattgat aacaaggtca ttt
//
LOCUS       DAOQMC010000017        16979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1212_length_16979_cov_4.897719, whole genome shotgun sequence.
ACCESSION   DAOQMC010000017 DAOQMC010000000
VERSION     DAOQMC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16979
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1212_length_16979_cov_4.897719"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2550)
                     /locus_tag="PKV31_01395"
     CDS             complement(<1..2550)
                     /locus_tag="PKV31_01395"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNB91533.1"
                     /translation="MLLFIRRFLLTSLGIFLSFSIFAQFTVNTSVTAMAMAQELVGPG
                     VEVISATYSGDPSSKGIFAATGTSLPMTNGVVLSTGYVTNIAGNASAQASTSTSGGSN
                     DPDLKTLVNNCANCVKDVAYLEFQFKAIGTEINFDFVFGSEEYPEYVCSSYNDVFGFF
                     ISGPGITGKKNLALVPGTTIPVAINTINSGTAGTFGTYSICLAACNGNPPYSQYYIDN
                     SGSTTIKFDGLTTTLTAHQEIQTCEIYTIKLAIADITDRLYDSGVFIKSNSFSSEVPT
                     LSIDATTSFPTCPVDPITFTSTLTGADPPINYFWFVNGIEQNVNSDQISINGLQNGDV
                     VWLVVSADINCSGTKLESNRITINFLPYGQEELNVSICEGDSYTFNGMTYTSTTLGVK
                     DTVPDPPNCDKIITLNLTVNPNVTPLFPNYGPFCQGDTPPGLPNTSTNGITGTWNPSV
                     INTSIPGTTSYTFTPDGTQCSSPVTIDVTVDAPEMPNFNPIGPFCKGDTAPGLPTSST
                     NGITGTWSPSVINTSTSGIFSYTFTPTSGLCADIVTINIEITEPIVPSFDPIGPLCQG
                     DPPPSLPNPSNDNPGISGKWVPGTINTTVPGTFTFNFIPNSGQCALPTSINITIEPKV
                     TPTFNQLGPYCKDETPDPLPISSSNGVSGTWNPSTINTSTPDSYTFTFTPDAGECAEG
                     TTMTIVIETPVIPVFNQLGPFCKDDVPPTFSNTSNNGISGSWNPAIINTSTPGTFSYT
                     FTPDVAYCAEPITMDIVIDPPVLPTFNPVDPLCQNSTPIVLPNTSTNNPAITGAWSPA
                     NVNTSTVGTFSYTFTPTTGQCALPATLDITIKAPVTPTFNAIGPLCQNQTPP"
     gene            2922..4190
                     /locus_tag="PKV31_01400"
     CDS             2922..4190
                     /locus_tag="PKV31_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNB91534.1"
                     /translation="MSQVNYSLIRIEHLSLRLKSSQLSELINNALRMALQKKDSFAVN
                     ILNDQEILITPQPSVTINIEDNKIKGILPLRISTNLKGPIKRVSVKAGILAHFEAEIE
                     RLDENNLGIHSDISAIEWTDEIDIQPPLLDFVLRDSFVKEMVENQLPAINIKINEALR
                     DFLDFGNLLSQAELNRKIRIPLKNKQEMFLFVSPESIEVNKMGLDGDNLQFSCNISGR
                     ILPSENMDDTFPTVTPRVIFLDQPASGNELLIDIRLDLASLGEPVLDVVKSMPNIENA
                     IGCNIEKVVVRPVGTDGVAIGLKLKGALNGGLSIAGRPVVDQNSLMLDIENLTFDFSG
                     KNILSSLKGNIALSVAKSVIRKQFPIGLRPYIDSMIRATNDMISSFKLIDGLILQGNI
                     GEWHLQEMDIVDGRLVIIFRTEVVTFLSPA"
     gene            complement(4192..4893)
                     /locus_tag="PKV31_01405"
     CDS             complement(4192..4893)
                     /locus_tag="PKV31_01405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB91535.1"
                     /translation="MVIVVTGSTRGIGKAITERFAADASIIFITARNEKALTEQESRL
                     KSDYPDLVVRKFAADLSKHDNVVAFARFVLDNCTAVDILVNNAGIFTPGNLLNEDEGN
                     FDLVMETNVRSIYTLCRMLVPKMIEARKGHLFNICSVAGLKAYPNGGSYSVSKFALLG
                     LTKNLRFELAPHGVKATAVIPGATWTDSWSNSGLPRSRFMEADDIAAMIYHISRLSPA
                     CVVEEIVLRPQLGDI"
     gene            complement(4898..6115)
                     /locus_tag="PKV31_01410"
     CDS             complement(4898..6115)
                     /locus_tag="PKV31_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNB91536.1"
                     /translation="MNIRILLTLSLLTLCKFSLSADHFRIIGYLPTYRFATLEKLEVE
                     KLTHLNLAFAQATYDGKLVLGGNFNYIVSRSKERNPDLKVLVSLAGGGIKGATFANWR
                     RNMAPGRKTEFIRQIMDFVRKNNFDGVDVDLEWGNVTPEYPEFVAELSDSLRSNNLEI
                     SAAFPAMKLYSVLNPESLKMFDYINIMAYDNSGPWQPRVVRQHSSYELAQNSIDFWAE
                     VCQLPKEKIVLGIPCYGWEFGKSVKSKTFSSLVSEKKEFAFTDNIRKIFYNSIPTIIE
                     KTKLARERCGGVMLWEIGQDAIGDKGDYSLLHFVHTTALTEGRNFLPKIRKFTIEQSN
                     ESIVSIRLEDQVAPGSYLVLEDIIGTANQKIDLCHGDSEVNVDLTIYPRGVYVISLIE
                     PGALPRSQRITKL"
     gene            complement(6176..6973)
                     /gene="fdhD"
                     /locus_tag="PKV31_01415"
     CDS             complement(6176..6973)
                     /gene="fdhD"
                     /locus_tag="PKV31_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNB91537.1"
                     /translation="MEEKLIIPVNIERLSEDMVFSKQDVVMIEQPLQMDALGPEGNMS
                     RISITMRTPGHDAELALGFLFNEGLLQTGDAASVANHTDNNILIKMNHNIQGQASKRN
                     FLMTSSCGVCGKSELNDLKINAPVRKIVHTLDHRNISSMYEWLKKEQKAFAETGGCHG
                     AGVFDYEGHMITGREDVGRHNAVDKCAGYLWENNLLEKEEYVLCLSGRGCYELIQKAI
                     RMNCTTVICIGAVTSLAIETAYQYGITLIGFFKPYNFNIYTYKERII"
     gene            7089..7991
                     /locus_tag="PKV31_01420"
     CDS             7089..7991
                     /locus_tag="PKV31_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB91538.1"
                     /translation="MGNKKAFLALVLICIIWGTTYLFLKIGVDHMEPFFFSGIRQTCA
                     GVLLLLIMWFLGKFEKITWRQILMQTVAGLLMITLGNGLVAYAEVSIPSSMAAVICAT
                     MPVWVSLINLLSPGAIRLTGWGYLSVMVGVAGIFWLFSDSIGDFGNKAYIYGALLTLL
                     ATLAWIAGSYILRRGGNNNHPFINSGIQMATGGIGLFAISFVLGESHHLSTDSQGWVA
                     LIYLTLIGSLVAMAAYSYALKHLPMPIVSVYAYVNPVVAIILGWMILGESMTLNMLLA
                     CMVIIISIILLNQPFSRKPATAVA"
     gene            complement(8050..10833)
                     /locus_tag="PKV31_01425"
     CDS             complement(8050..10833)
                     /locus_tag="PKV31_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNB91539.1"
                     /translation="MNKIYILFLLLIIPALSYSQIDNKDMKMLMLQNSGKIDKIKADI
                     KAKGITEAELSSKLKQKGIDLQNLQPEQIPTLELMVDEAIREIENEKIKAKSEIETKP
                     STSDKKEKEVDLDNFEFSRNFTKSIEESFDLKGNKQKTARFVSDSLKAADTIPFAIFG
                     QQIFKYGNPKLFTSPDDINPANDYLLGPGDVIRIVIFGRSLYDGNLEVDEQGYIDPKT
                     LPQIFVKGIEWGKVKKLIEARYKQRYSFNTGEIAITLKKTRNITVSVFGEVVSPGSYT
                     YSAANTAFNLIALAKGLTDLGSVRNIQIVTPGGKVRTYDVYRAMMDPLQAANIYLQNN
                     DFIQVPVAQKVVSINGSVNRPHRYELLPKEGLMDLIKYAGGFKADAYLDNIQIIRFEN
                     GARKIYSVKYTESQKFILQNGDEVTVIPSSGEVKNMLTIVGDIERPGEFEWKQGMRVS
                     DVVTLAGIKRTTRMDLAYIKRLNPDNSFNLLAFSPDTVSRNVHNLRNFLLHPNDKLIF
                     LSQADFTQYYKVEVNGAVRMPGEFEYDPARNISVYDAVNLAGGLRPDALPKAYVVRTD
                     TATGARTYALFNLEKAMTDLTSNDNFSLEPLDRIVILSNSIINTESTVSIGGAVKNPG
                     KFKYGNSMTLLDAVTLSGGLKAEAATNRVEIYRVILNNNEPTQVTASTFEIPRDLIMA
                     QEIMSHVLLEPFDMIEVRSVPKFGLQKVVSITGEVRFPGEYALVKDNETIYELIKRAG
                     GLTPEAFTGGATLDRNMDKTGLVIIELDEIMKRPGASYNITLLHGDALNIPKNNDLVS
                     IQGAVNIQDIASQELINENHKINVAFIEGKSARYYIREFAGGFAENADRAKVYVKDAN
                     GKKSKVHTFLFFKKYPKVKKGSSIVVDYKDPKKIRTEPKEKTDWVKVLGESVAQATAI
                     LTLVVLINTINKS"
     gene            complement(10921..11859)
                     /locus_tag="PKV31_01430"
     CDS             complement(10921..11859)
                     /locus_tag="PKV31_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB91540.1"
                     /translation="MKKVLITGAAGFLGSHLCDRFIREGNHVIGMDNFITGNPDNIAH
                     LFPLKHFEFHHHDVSKFVHITGHLDYILHFASPASPIDYLKMPIQTLKVGSLGTHNLL
                     GLAKAKNARMLIASTSEVYGDPLVHPQREDYWGNVNPIGPRGVYDEAKRFQEAMTMAY
                     HNFHGLETRIVRIFNTYGPRMRVNDGRALPAFFSQAIKGQNLTVFGDGSQTRSFCYVD
                     DLVEGIYRLLLSDYHLPVNIGNPEEITLNQCADEVIEIVGNPEVKIEYHDLPKDDPKV
                     RRPDITLARQLLGWEPTVSRNEGLRATFEYFKKALS"
     gene            complement(11856..12965)
                     /locus_tag="PKV31_01435"
     CDS             complement(11856..12965)
                     /locus_tag="PKV31_01435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNB91541.1"
                     /translation="MDQKIPMNDLGAQYLRYKDEIDRAIQNVIDSGQFIKGPIVNQFE
                     ADLSKYLGGVEVITCGNGTDALQIALMALDLKEGDEVITTPFTFAATAEVIALLKLKP
                     VFVDIKPDTYCIDPTKIEEAITPATRCIIPVHLFGTIADMNAIMAIADLYGLYVIEDA
                     AQSLGSLYKMNDGNTVHSGTIGHIGTTSFFPSKNLGCFGDGGALFTKDRVLAEKIRMI
                     ANHGTKVKYNHEIIGVNSRLDSIQAAILNVKLQYLDMDISARIKAAAIYDQLLHGHGE
                     ITSPIATENATHSYHQYTVRVKNRSKVRVRLGEAGISSMIYYPIPLHIQKAFAFAGGV
                     IGRFPVTECACDEVLSLPIYPDITKEKIEKVVAHL"
     gene            13068..13823
                     /locus_tag="PKV31_01440"
     CDS             13068..13823
                     /locus_tag="PKV31_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91542.1"
                     /translation="MVFCKRLIYIIVLCHILVVSAISQEKTSKARSALLIHLDYGFNL
                     PAADLAKRFGSNFQVGGDVEYKFPVDIFIGVNYSYLWGKKVKENIGENLFNAKNEIVG
                     IDNHLANLVIRERGFSSSLNVGKLFRIFPNEAAALKVGIGGGFLQHKVRIVDDYGVVP
                     QITGDYLKGYDRLTNGFMTRQYIGYQYLSTNRKINFSVGFVFAQGFTRSARGFNYDTG
                     KKDTEKRFDLLNGLQVSWILPIYFNDYSEEIIY"
     gene            13832..15076
                     /locus_tag="PKV31_01445"
     CDS             13832..15076
                     /locus_tag="PKV31_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNB91543.1"
                     /translation="MAYILYNFGIRIYHLLFLCYSCINSKAAKIRIGRKQLIRYLGST
                     SSGLPTVWFHCSSLGEWEQTIPVVERLKEKSNVQILVSFYSSSGFEYAKRRDLVDAMI
                     YLPVDFYKAYAPVFNTLNLVGISFTRYDIWPNLVRHAVDRNVPLFLIGAESASLRKYR
                     RSNGFFAKMLRSFSFISCNTVSAATLLNNSGYRHVYTDGSSKLERSAQIAAQEYVNAS
                     LDSWRKDAFVIIGGSIWPPEAIMMERLFIEGAIENMKMILVPHEPSHDVIGALQEKYK
                     DTRVLFSQMDGNSTESKVIIMDKVGFLSKIYRFADVAIVGGGFGKGIHNIAEPAASGL
                     AVISGPNNSGFSEAAMLQDAGVYFPIETYKEFRELVIDFAENELKRKEIRTIANTFFN
                     AQSEAAERISQHIVDYAGITER"
     gene            15054..16037
                     /locus_tag="PKV31_01450"
     CDS             15054..16037
                     /locus_tag="PKV31_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNB91544.1"
                     /translation="MPELLKDKFTSLNICVIGDLILDEYIIGKVTRVSPEAPIPVVDQ
                     QAREYKPGGAANVALNLINLGVNTYLAGVVGDDTHGLILKKLIEEVLPEKGQGIITLK
                     GRITTTKTRVVAHNQHVVRIDHEEHTDIGNDVSAQLMDYIADIHKNNKLDAVILQDYE
                     KGMLHHANIPAFIEFLNECGIRIIVDPKDKNFWMYRHVHLFKPNRKEAEKAIGKKIPM
                     TEDALADAALLLESRLHNEVTVITLSEYGIYIKDAENNVWRHSEVMEVIDVCGAGDAV
                     IGIVAAAYCAGLPMPQIALLGNIAGGIVCGERGVVAVSLDRLQKAYNNMSL"
     gene            16141..>16979
                     /locus_tag="PKV31_01455"
     CDS             16141..>16979
                     /locus_tag="PKV31_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91545.1"
                     /translation="MRKFLTLKVSMSFWMLLGFLSVGNIAMAQSFYIEFLSPAEIRGQ
                     RVNFVAGASTVVCHLPFGDSLNTQLTDPILAGDPNDPNNTQFTFAEALNGNVGVCKRG
                     SFSFNVKASNIEFNGGSGSIIINNDQANKDAIISMSQDTSFSIWSVAVSFNSGENFFL
                     KYPGLSFKFVNDFPAETDNILWSDNGFSGGLDGWTTVGVACGDGKDPVGAEWEWTEFG
                     SSKGAYGSTNILSATACNGAMTFNTDFKDNDGVQGNFGGGICGVNQIGELISPDIDLS
                     ALP"
BASE COUNT         4624 a         3350 c         3738 g         5267 t
ORIGIN      
        1 aggtggagtt tgattctggc aaagtggtcc gattgcgttg aaggtcgggg taactggtgc
       61 cttaatcgtt atatctaaag tggctggtaa ggcacattgc cctgtagtag gtgtgaaggt
      121 ataggagaag gtacctacgg ttgatgtatt gacattggcc ggtgaccagg cacctgttat
      181 tgcgggattg ttagtcgatg tgttgggcaa tacgataggt gttgagttct ggcaaagtgg
      241 atccaccgga ttgaatgtgg gtaagacagg tgggtcgatt acaatatcca tggttattgg
      301 ttcagcacag taagcaacgt ccggagtaaa cgtgtagcta aatgtaccgg gcgtagaggt
      361 gttaattatc gccggattcc agctccctga tataccgtta ttagaggtgt tggaaaaggt
      421 tggaggtaca tcatctttac agaatggccc tagctggttg aacaccggta ttacgggtgt
      481 ctctatgact atggtcatgg tcgtgccttc agcacattct cctgcatccg gcgtgaatgt
      541 gaaggtataa ctatcgggtg tggacgtgtt gatggtcgat gggttccatg ttcctgacac
      601 accattggaa gaggatatcg gaagtgggtc aggagtctca tccttacaat aaggcccaag
      661 ttggttgaat gtcggagtga cttttggctc tatggttatg tttatcgatg tgggtaaggc
      721 acattgtccg gaatttggta taaaattgaa tgtgaatgtg cccggtacgg tggtgtttat
      781 tgttcccggt acccattttc ctgaaattcc cggattgtca ttcgatggat taggtaaact
      841 tggtggcgga tcaccttgac ataagggacc tattgggtcg aatgaaggca ctatgggctc
      901 agttatttcg atgttgatgg tgacgatatc agcacatagc cctgaggtag gtgtaaaggt
      961 ataagaaaat attcccgagg tggaagtatt gattactgaa ggactccatg ttccggtaat
     1021 tccgttagta gaagatgttg ggaggccggg agcggtgtca cctttgcaaa aaggtccaat
     1081 aggattgaaa tttggcatct caggagcatc tacggttaca tctattgtta caggagaact
     1141 gcattgtgtc ccatccggtg taaaagtgta actggtagtg ccgggaatgg aagtattgat
     1201 tactgaggga ttccatgtcc cggtgatgcc gttggtggag gtgttgggca aaccgggagg
     1261 agtatctcct tggcagaaag gtccataatt tggaaaaaga ggcgtgacgt tcggattgac
     1321 cgtaagattc aaagtgatga ttttgtcaca atttggtgga tcagggactg tatcctttac
     1381 tcccaatgtc gtcgaggtat aggtcattcc attgaaggta taactatccc cctcacatat
     1441 cgatacattt aactcctctt ggccataggg tagaaaatta atggttattc tgttgctctc
     1501 aagttttgtt ccgctacaat tgatatccgc actgacaaca agccatacca catccccatt
     1561 ttgaagccca ttaatggaaa tctgatccga gtttacattt tgttcaattc cattgacaaa
     1621 ccaaaaatag ttgattgggg gatctgctcc ggttaatgtt gaggtaaatg taattgggtc
     1681 gacagggcaa gtaggaaagg atgttgtagc atcgatggat aaagtaggta cttcggaaga
     1741 aaaactgtta cttttgatga atactccgga atcatacagc ctatcggtga tatctgcaat
     1801 ggctaatttt atggtataaa tttcgcacgt ctggatttcc tgatgtgcag tcaaggtcgt
     1861 agttaatcca tcaaacttga tagtagtact accggagtta tcgatataat attgcgaata
     1921 tggtgggttg ccattacacg cggccaagca tatggaataa gtgccaaatg taccggcagt
     1981 tccgctattg atggtgttga tagcaaccgg tatagtcgtt ccgggaacca atgccagatt
     2041 ttttttaccg gttatccccg gaccactgat aaaaaatcca aatacatcat tataggaaga
     2101 acaaacatat tccgggtatt cttcagagcc aaatacaaaa tcaaaattta tttctgttcc
     2161 aattgcctta aattgaaatt caaggtaagc aacgtctttt acgcaattgg cacaattgtt
     2221 gaccagggtt ttaaggtcag gatcattcga tcccccactg gtagaggtac tagcctgtgc
     2281 acttgcattg ccggcaatat ttgtcacata gcctgttgaa aggactacac catttgtcat
     2341 tggcaatgaa gtgcccgttg cggcaaatat acctttggaa cttggatcgc cggaataagt
     2401 agcggaaatc acttcaacac cgggacctac taattcctgc gccatagcca ttgccgtgac
     2461 acttgtgttg accgtaaatt gagcgaaaat ggaaaaactt aaaaaaatcc caagtgaagt
     2521 aagtaaaaat ctccgaataa ataaaagcat gatcgtctta aaaaaagtcc aaaaagagat
     2581 gactgaaatt ataattcgtt tttacaaatg taaatgtaat aaaattgtat gtaaaataat
     2641 ctttattgaa gaatttctat aagaattgag tgcaagaaca taaaagaatt ggtaggtatt
     2701 attttgatta aaagaattat atttattaaa atatgtatac tgaaaaatta aattatgtat
     2761 ttgcgtaatt gatgtatatt ttgagttgtc gtgtatttat agaattatca aaagagaaat
     2821 atttttagtc gaacaacgag attcttggga aaaataattg tcccggaaat ttttggaaaa
     2881 tacttcaact aattttgtat aaatcatatc ttagcacctg tatgtcacaa gttaactatt
     2941 cattgattag aatcgaacat ttgagtttgc gattgaagag cagccagttg tctgaactta
     3001 taaacaatgc tctgagaatg gcattgcaaa aaaaagattc atttgcagtc aatatcctca
     3061 acgatcagga aatcctgatt accccacagc caagtgttac gatcaatatc gaagataaca
     3121 agatcaaggg gatcttgccg ttacgaataa gtaccaacct gaaaggacca atcaaacggg
     3181 taagtgtaaa ggccggtatc cttgcgcatt ttgaggctga gattgagaga cttgatgaaa
     3241 ataatctggg catccattct gatatttcag cgattgagtg gactgatgaa atagacatac
     3301 agccaccttt gctggatttt gtcctgcgtg atagttttgt gaaggaaatg gtggaaaatc
     3361 agttacctgc catcaatatt aagatcaatg aagcgttgag agactttctc gattttggta
     3421 atttactgtc ccaggccgaa ttgaatagga agatcagaat accattaaag aataaacagg
     3481 agatgttcct ttttgtatcg cctgaatcaa ttgaggttaa taaaatgggg ctggatggtg
     3541 ataacttaca gtttagttgc aatatttcag gtcgaatcct tccctctgaa aatatggatg
     3601 acacatttcc cactgtcaca ccccgggtta tttttcttga ccaacctgca tccggaaatg
     3661 aattgttgat cgatatacga cttgatttgg cgtcgctggg tgagccggtt ctggatgttg
     3721 tgaagtccat gccaaatatt gaaaatgcaa taggatgcaa tattgaaaag gtagtagtcc
     3781 ggccggtagg gaccgatggt gttgccattg gattgaaact gaaaggagca ctcaatggtg
     3841 gtctgtccat agcaggtcgc ccggtagtcg atcaaaattc gctgatgctc gatattgaaa
     3901 atctgacttt tgacttcagt ggtaaaaata ttctttcctc actcaaggga aacatagcgc
     3961 tttcagtagc caaatccgtc atcagaaaac aatttccaat tggattgagg ccgtatatcg
     4021 atagtatgat ccgtgccacc aatgatatga tttcttcatt caaattgatt gacggcctta
     4081 tactgcaagg caatatcggc gaatggcatc ttcaggagat ggatattgta gatggtcgtc
     4141 tggttattat tttccgtacg gaagttgtga cctttttaag tccggcatag atcaaatgtc
     4201 ccccagttga ggccgcaata cgatttcttc cactacacac gccgggctta atctcgatat
     4261 gtgataaatc attgcagcga tgtcatcagc ttccataaac cttgatctgg gcagacccga
     4321 attggaccag ctgtcagtcc aggttgcacc cgggatgact gccgttgcct tgacaccatg
     4381 aggggccagt tcaaaacgta aattcttcgt taaacccaaa agggcgaatt tcgacacaga
     4441 ataagaacct ccatttggat aggcctttag ccccgcaaca gaacagatat tgaaaaggtg
     4501 gccttttcgg gcctcgatca tcttaggaac cagcattcga catagtgtat aaatacttct
     4561 tacattggtc tccatcacca gatcaaaatt accttcatct tcatttaata aattacccgg
     4621 agtaaatatt ccggcattgt tgaccaaaat atccactgcg gtgcaattgt ccaatacaaa
     4681 tcgtgcaaag gcaaccacat tgtcatgttt agagaggtca gcggcaaatt ttctaacaac
     4741 caggtcagga taatcactct ttaatcgact ttcttgctct gttaacgctt tttcgtttcg
     4801 cgccgttatg aagattatgg atgcatccgc tgcaaacctt tctgttattg ccttacctat
     4861 gcctcttgtg ctacctgtaa ctacaataac catgaggcta taattttgta attcgttgac
     4921 ttctgggcaa tgctcccggt tcaatcagag agatgacata gactccgcga gggtagatag
     4981 tgagatccac attgacctca ctatctccat gacaaagatc tatcttttga ttggcggtac
     5041 caatgatatc ctccaggacc aaataagatc ctggtgctac ctggtcctcc aacctgatgg
     5101 atacgatgct ttcattactt tgttcgattg taaattttct tattttgggg agaaaatttc
     5161 tcccttctgt taatgctgta gtatgaacaa aatgtaatag agaataatca cctttatcgc
     5221 caatagcatc ctgaccaatc tcccataaca tcacaccacc gcatctttct cttgccagct
     5281 tggtcttttc tattattgta gggatgctat tgtaaaatat ctttctgatg ttgtccgtaa
     5341 aagcaaactc ctttttttca ctaaccaggg atgaaaaagt cttgcttttg actgacttgc
     5401 caaactccca cccataacat ggtatgccga gaacgatttt ttcttttgga agctgacaaa
     5461 cttctgccca gaaatcgatg gaattttgag caagttcata ggaagaatgt tggcggacta
     5521 cccttggctg ccacggacca ctgttgtcat aggccatgat gttgatataa tcaaacattt
     5581 tcagactttc gggattcagc acgctgtata gtttcatagc agggaaggca gcacttatct
     5641 ccaggttgtt gctgcgtaga gaatcactta attccgctac aaattcaggg tattcaggag
     5701 taacattgcc ccattccaga tcgacgtcca ctccgtcgaa gttattcttt ctgacgaagt
     5761 ccataatctg acggatgaat tcagtctttc ttccgggtgc catattcctg cgccaattgg
     5821 caaaggtggc acccttgatt cctcctccgg ccaatgacac caaaaccttt aagtcaggat
     5881 tccgttcttt ggacctcgat acaatataat tgaaattgcc acccaaaacc agtttgccat
     5941 cataagtcgc ctgggcaaaa gcgagattaa gatgtgtcaa tttttcaacc tccagttttt
     6001 caagcgttgc aaaccgatag gtagggaggt agccaataat cctgaaatga tcagcggaaa
     6061 gtgagaattt gcaaagggtg agaagcgaaa gagtcaataa aatccttata ttcatcctgc
     6121 aatgtattaa catacaatgc gcaaatataa ttaaatttca tatatataaa aatttttaaa
     6181 ttattctttc tttatatgtg taaatattga aattatatgg cttaaagaat ccgatcaggg
     6241 tgatgccgta ctgataagct gtttcaatag ccaaactggt caccgcacca atacagatta
     6301 ctgttgtgca attcatacgg attgcctttt ggatgagttc ataacaaccc ctgccactta
     6361 ggcaaaggac atattcttcc ttttccagta aattattttc ccatagatag ccggcacatt
     6421 tatccacagc attatgccgg ccaacatctt ctcttcctgt tatcatatgc ccttcgtagt
     6481 caaatactcc ggcaccgtgg cagccacctg tttcagcaaa agctttttgt tcttttttca
     6541 accattcata catggatgag atgttcctgt gatccaaagt gtgaacgatt tttcttaccg
     6601 gtgcatttat ttttaaatca ttgagttctg atttaccaca tacaccgcaa ctggaagtca
     6661 ttaaaaaatt tcttttggat gcctgtcctt gtatattgtg gttcattttt atcaaaatat
     6721 tattatcggt gtgatttgcg actgatgctg catcacctgt ttgcaatagg ccttcattga
     6781 aaaggaagcc taaagccaat tcggcatcat gacccggagt gcgcatggtg atgcttattc
     6841 tggacatgtt accttctgga cctaatgcat ccatctgcaa aggctgttcg atcatgacaa
     6901 cgtcttgttt ggaaaacacc atgtcttcgc taaggcgttc aatatttaca gggataatta
     6961 atttttcttc catgattcta atctttttgc atctcttcgt gttcctaaag gatgattcag
     7021 ggacttgggt ttacaaaata acaaattttt tcgtaatttg atttttcaag cgaattaatt
     7081 aatgctgtat gggtaataaa aaggcatttt tagctttggt gttgatttgt atcatctggg
     7141 gaacgactta tttgtttctc aagattggag tcgaccatat ggaacctttt ttcttttcag
     7201 gtattcgtca gacttgtgcc ggtgtgctgc ttctattgat tatgtggttt ttgggcaaat
     7261 ttgagaagat tacatggcgc cagattttga tgcagactgt tgccggtttg ctcatgatca
     7321 ctctgggtaa tggtcttgtt gcctatgctg aggttagcat cccaagcagt atggcagcgg
     7381 tcatttgtgc tactatgcct gtgtgggtga gcttgatcaa tctgctatcc ccgggtgcaa
     7441 tccggctgac cgggtggggc tatctttcag tcatggttgg agtggcaggc attttctggc
     7501 tgttttccga ttctattggt gactttggta acaaggctta catatatggc gctcttctta
     7561 cccttttggc gacattggca tggattgcag gaagttatat cctcagaaga gggggtaata
     7621 ataatcatcc atttatcaat tccggcatac agatggccac cggtggaatt ggtctttttg
     7681 caatatcgtt tgttttgggc gaaagtcatc atttgtcaac ggattcgcaa ggatgggtgg
     7741 ctctcatcta cctcaccctg attggctctt tggtagctat ggcggcctat tcctatgcat
     7801 tgaagcatct acccatgccg attgtatcgg tctacgctta cgttaatccg gtcgtagcca
     7861 tcattcttgg ctggatgatc ctgggtgagt ccatgacttt gaatatgctg ttggcttgta
     7921 tggtcatcat aatcagcatc atcttgctga atcaaccatt ttccagaaag cccgccactg
     7981 cagttgccta aattaaggag gcaagccgta ttatgatcgc ttgataatca ggcctaatgt
     8041 gtttccttgt caactcttat tgatggtatt gatcaacaca accaaagtaa gtatggctgt
     8101 tgcctgtgcg acagattcac ccaatacctt gacccagtcg gtcttttcct tgggttcagt
     8161 cctgatcttc ttgggatcct tataatccac cactatactt gagccttttt tgaccttcgg
     8221 atatttcttg aagaagagaa aggtgtgaac ctttgatttt ttgccgttag cgtcttttac
     8281 atatactttc gctctgtcag cattttccgc aaaacctccg gcaaactccc tgatataata
     8341 tctggcagat tttccttcga taaaggcaac atttattttg tggttttcat ttataagttc
     8401 ctggcttgcg atatcttgaa tatttactgc tccctgtatg gataccagat cattgttctt
     8461 agggatattc aaggcatcgc catgtaatag ggtgatattg taggaagcgc cgggccgttt
     8521 catgatttca tccagttcga tgatcacgag acctgtttta tccatatttc ggtcaagagt
     8581 tgcacctcct gtaaacgctt ccggggtgag gccaccggcc cgtttgatta gttcgtatat
     8641 cgtctcattg tctttgacaa gggcatactc accaggaaac ctgacttcac cggttatgga
     8701 cactactttt tgaagaccga attttggcac tgaccttact tcaatcatat cgaacggctc
     8761 aagtaggaca tggctcatga tttcctgtgc cattatgagg tcacgaggaa tttcaaaggt
     8821 ggatgccgtt acctgtgtcg gttcattgtt gttaagtatg actcgatata tctccactct
     8881 gttggttgct gcttcggctt tcaatcctcc actcaaagta accgcatcca ataaggtcat
     8941 ggaattaccg tatttgaatt tgccgggatt tttaacagcg ccacctatag atacagtact
     9001 ttcggtatta atgatggaat tggataatat cacaattcta tccaatggct ccaacgaaaa
     9061 attgtcgttg gatgtaagat ctgtcatggc cttttccagg ttgaacaatg cgtacgttct
     9121 tgctcccgtt gctgtatctg tcctgactac ataggctttc ggtaaggcat ccggcctcag
     9181 tccaccagcc agattcaccg catcatatac actgatattc cttgcgggat catattcaaa
     9241 ctcaccgggc atacgcactg cgccatttac ttctacttta taatattggg taaaatctgc
     9301 ctgggataaa aaaatcagtt tatcattagg atgtaataag aaatttcgaa ggttatgtac
     9361 gtttctgctt actgtgtctg ggctgaatgc caaaaggttg aaactattat ccgggttgag
     9421 tcttttgatg taggccagat ccatacgtgt ggtcctttta attcctgcca gggttaccac
     9481 atcactgacc cgcatgcctt gtttccactc aaattctccc ggtcgctcta tgtctccgac
     9541 tatggtaagc atgtttttca cttctccgga tgaaggtatt accgtcacct catctccatt
     9601 ttgaaggatg aatttttgtg actcagtata ttttacggaa taaatttttc ttgcaccatt
     9661 ttcaaatcta ataatctgaa tattgtccag atacgcatct gccttaaatc ctccggcata
     9721 ttttatcaga tccatcaacc cttctttggg aagtaactca tatcgatgag gtcgattgac
     9781 ggaaccattg atggatacaa ctttttgggc cacagggacc tggatgaagt cattgttttg
     9841 cagataaata ttagcggctt gtagcggatc catcatcgct ctatatacgt catacgtgcg
     9901 aacttttcct cccggtgtaa caatctgaat atttctcaca ctaccaagat ctgtcagacc
     9961 ttttgcaaga gctataaggt tgaaggctgt attggcggct gaataggtat aagaaccggg
    10021 gcttactact tctccgaaaa cactgacagt aatatttcgg gtctttttca gagtaatcgc
    10081 aatctcaccg gtattgaatg agtatctttg cttgtatcgc gcttctataa gctttttgac
    10141 ctttccccat tcaatccctt tcacaaatat ttgaggtaag gtttttggat caatgtaacc
    10201 ttgctcatca acctcaagat tgccatcata gagcgaccgg ccaaatatga cgatcctgat
    10261 cacatctccg gggcctaaaa gatagtcatt ggcgggattg atgtcatcag gggaagtgaa
    10321 gagcttggga tttccatatt tgaatatttg ctggccgaag attgcaaatg gaatggtgtc
    10381 cgccgctttt aatgagtcac ttacaaaacg ggcagttttc tgcttattgc ccttaagatc
    10441 aaaggattct tcaatggatt tggtgaaatt cctggaaaat tcgaaattat ccaggtcaac
    10501 ttccttttct tttttgtccg acgtagacgg cttcgtttca atctcagatt tggcctttat
    10561 tttttcattt tcaatttccc gaatggcttc gtcaaccatc aattccagcg ttggaatctg
    10621 ctcgggttgc aggttctgaa ggtcgattcc tttttgcttt aatttacttg aaagttctgc
    10681 ttctgttatg ccctttgcct ttatatccgc ttttattttg tctatcttgc cactgttttg
    10741 caacatcaac attttcatgt ccttattgtc tatctgcgaa tatgacaatg caggaataat
    10801 aagtaaaaga aataatatat atatcttatt catatatgta agttggttat agcattaaaa
    10861 gccattcatc atttcaggct gcaaatttaa taccattttt ctgttattta atattttagc
    10921 ttatgacagg gctttcttga aatattcgaa ggtagctctt aatccctcat tcctgctgac
    10981 tgtaggctcc cagcccagca attgccttgc cagcgtaata tccggcctcc ttaccttagg
    11041 atcatctttc ggcaaatcat ggtactcaat tttgacttcc ggatttccta cgatctcgat
    11101 tacttcatcg gcacattggt tgagggtgat ttcctccgga ttgccaatgt tgacaggaag
    11161 atgataatcc gacaataaaa gtctgtagat accttctacc agatcatcga cataacaaaa
    11221 cgatcttgtc tggctgccgt ccccaaagac ggtcaggttt tgtcctttta ttgcctgact
    11281 aaagaatgca ggtaacgcgc gcccatcatt gacacgcatg cgaggtccgt aggtgttgaa
    11341 aatccttaca atacgtgttt ccagcccatg gaagttatgg taggccatag tcattgcttc
    11401 ctgaaaacgc tttgcttcgt catacacccc tctgggcccg attggattta cattacccca
    11461 gtaatcctcc ctctgaggat gcaccagcgg gtcaccatat acctcggatg tcgatgcaat
    11521 cagcatccgt gcattcttag ccttggcaag tcctaaaaga ttgtgtgtgc cgagggagcc
    11581 cactttgagt gtctgaatag gcatcttcag atagtcaatt ggtgaagccg gtgatgcaaa
    11641 atgaaggata taatccagat gacctgttat gtgaacgaat tttgagacat cgtgatggtg
    11701 gaattcaaaa tgttttaacg ggaaaagatg ggcgatgtta tccggattgc ccgttataaa
    11761 gttgtccatc ccgatgacat gattaccttc tctgatgaat ctgtcacaga ggtgggaacc
    11821 caaaaatccg gcagctccgg ttatcagtac ttttttcata gatgtgctac tactttttct
    11881 attttttcct tggtgatatc ggggtagata ggaaggctca atacttcatc acaggcacat
    11941 tcggtcactg gaaatcggcc gatgacccct cctgcaaagg caaacgcctt ttggatatgc
    12001 aagggtatcg ggtaatagat catggaagat atgcccgcct cacccaatct gacccttacc
    12061 ttggatctat tcttgacgcg aacggtgtat tgatgataac tgtgagtggc attctccgtt
    12121 gctataggcg aagtaatctc tccatgccca tgaagcaatt gatcatatat tgcagctgct
    12181 tttattcttg cactgatatc catgtcaagg tattgcagtt taacattcag tatggcggct
    12241 tgaatagagt ccagtctact gttcaccccg atgatttcat gattgtactt gacctttgta
    12301 ccgtgattgg caatcattcg tatcttttcg gccaataccc tatctttggt aaacagagcc
    12361 ccaccatcac caaaacaacc taggtttttt gagggaaaga agcttgttgt ccctatatga
    12421 ccgatggttc cggagtgaac agtattccca tcattcatct tatacaatga tcccaaagat
    12481 tgggcagcgt cctctatcac ataaaggcca tataagtcag caatggccat gatagcattc
    12541 atatctgcaa ttgttccgaa aagatggacc ggaatgatac atctggtggc cggtgttatt
    12601 gcctcctcga tttttgttgg atcaatacaa taggtgtccg gcttgatatc cacaaataca
    12661 ggtttcagtt tcaaaagagc tatcacttct gcagtcgcag caaaggtaaa aggcgtagtt
    12721 ataacttcat cgccttcctt cagatccaga gccatcaaag ctatttgcaa tgcgtccgtg
    12781 ccattaccgc aagtgattac ttcaacacct cccagatatt tagaaagatc cgcttcaaac
    12841 tgattgacaa ttggcccttt gataaactgt ccactatcga tgacattttg aatggcccgg
    12901 tcaatctcgt ccttgtatct taggtattgg gcacccaagt cgttcatcgg aattttttga
    12961 tccaaaagtt gtatgttttt ggtgtaaaag aaaaacaggg caaaagtaag ctattagttc
    13021 ctattaatta tgccatattt gatgattcaa tttactaact ttgcattatg gttttttgta
    13081 aaagattgat ttacatcatt gtcctgtgtc atatactggt tgtttcagcc atttctcagg
    13141 aaaaaacgag caaggcaaga agtgcgttac tcatacacct ggattatggc ttcaacctgc
    13201 ctgctgcaga tttggccaaa cgcttcggca gtaattttca ggttggtgga gatgtggaat
    13261 ataaatttcc ggttgatatc ttcattggag tgaattattc ctatttgtgg ggtaaaaaag
    13321 ttaaagagaa tattggtgaa aacttgttta acgccaagaa tgaaatagtc ggaattgaca
    13381 accatcttgc aaatcttgtc atccgggaaa gaggtttttc ttcttccctg aatgtcggaa
    13441 agttattcag aatttttcca aatgaagccg cagcactgaa agttggcatt ggtggaggct
    13501 ttttgcaaca taaggtgaga atcgtggatg attatggagt cgtgccacag ataactggcg
    13561 actatctgaa aggttatgac aggcttacca acggctttat gacccggcag tatattggat
    13621 atcagtatct atccaccaat agaaagatca acttttccgt tggttttgtt tttgcgcagg
    13681 gatttacccg cagtgcacgc ggatttaatt acgacaccgg caagaaagat accgagaaga
    13741 gatttgattt gctcaatggc cttcaagtta gctggatact gcctatttat ttcaacgatt
    13801 attcagaaga gattatctat tgacccaatc tgtggcctat atattatata atttcggtat
    13861 tcgaatttac cacctgttgt ttttgtgcta ttcatgcatc aattctaaag ctgctaaaat
    13921 tagaatcgga agaaagcagc tcatccgata tttaggatcc acatcttccg gcttgcctac
    13981 cgtttggttt cattgctctt ccctgggtga gtgggaacaa actatccctg tggttgaaag
    14041 attaaaggag aagtccaatg tccaaattct ggtgtccttc tacagttcat ccggttttga
    14101 atatgccaaa cggcgcgatt tggtggatgc aatgatttat ttgccggttg atttttataa
    14161 ggcttatgca cccgttttta atacattgaa ccttgtcggt atcagtttta cgcgttatga
    14221 tatctggcct aatcttgtca gacatgcagt cgacaggaat gtgcctctgt ttttgattgg
    14281 tgctgagagt gcatctttga ggaaatacag gaggtcaaat gggtttttcg cgaaaatgtt
    14341 gcggtcattc tcgtttataa gctgtaatac ggtaagtgcg gcaacactat tgaacaattc
    14401 gggatacaga catgtgtata ctgatggaag ttccaaactg gaaagatcgg cgcaaattgc
    14461 tgctcaagag tatgttaatg catccttgga tagctggagg aaggatgcat tcgtgatcat
    14521 cggaggtagt atatggccac cggaggctat catgatggaa aggcttttta tagagggcgc
    14581 tatagaaaat atgaagatga ttcttgtgcc tcatgagcct tcccatgatg tgatcggggc
    14641 tcttcaagag aaatataaag acacccgggt gcttttcagt caaatggacg ggaacagtac
    14701 ggagagtaag gtgattatta tggataaggt aggtttttta tcaaaaatat atagatttgc
    14761 agatgttgcc attgttgggg gaggattcgg aaagggaata cataatatag ctgagccggc
    14821 tgcttctgga ttggccgtga tttcaggtcc gaataattca ggtttttcgg aagctgccat
    14881 gctacaagac gcaggtgtgt attttccaat tgaaacctat aaagaatttc gtgagttagt
    14941 gatcgatttt gctgagaatg aattgaaaag aaaagaaata cggaccattg ccaacacatt
    15001 ttttaatgcc cagtcggagg cagcagagcg aataagtcaa catattgtgg attatgccgg
    15061 aattactgaa agataagttt acatcgttaa atatttgtgt cataggagat ttgatcctgg
    15121 acgaatacat catcggtaag gtaaccaggg tctcgcctga ggctcctatt cctgtggtgg
    15181 atcagcaggc gagggagtac aagccgggcg gagctgccaa cgtcgccctc aatttgatca
    15241 accttggcgt gaatacgtat ctggcaggtg ttgtggggga tgatacacat ggattaattt
    15301 taaaaaaatt gattgaggaa gtgttgcccg aaaaaggtca aggtatcata acattgaaag
    15361 gtcggatcac taccaccaag accagagttg tcgcccacaa tcagcatgtg gtcagaattg
    15421 atcacgaaga acacacggac atcgggaatg atgtaagtgc tcaactgatg gactatattg
    15481 ctgacattca taagaataat aaactggatg cagtaattct tcaggattat gaaaaaggca
    15541 tgttgcatca tgcgaatatc cctgctttta tagaatttct gaatgaatgt gggataagga
    15601 ttatagtgga tccgaaagat aaaaatttct ggatgtaccg tcatgttcat ttgtttaagc
    15661 ctaacagaaa agaggcagaa aaggcgattg gcaagaagat accgatgacg gaggatgctc
    15721 tggctgatgc ggccctactg ttggaatccc gattacataa tgaagtcact gtaattacct
    15781 tatcagagta tggtatatat attaaagatg cagaaaataa tgtctggagg cattcggaag
    15841 tgatggaagt gatcgatgta tgcggtgctg gagatgctgt aataggcatt gtggctgcag
    15901 cgtattgtgc aggcctaccc atgccccaaa ttgcactttt gggtaatatt gccgggggga
    15961 ttgtttgtgg tgagaggggg gtagtagcag ttagcttgga caggttgcaa aaagcatata
    16021 ataatatgtc cttataaaaa attaacgaaa tttatttttg agtattcaat tctttcctta
    16081 taattgtgtc ttaatttgta atcatttaat cttattacat taataaaaaa gtttagtatt
    16141 atgagaaaat ttttgactct aaaggtgtca atgagttttt ggatgttgtt gggctttctg
    16201 tctgtaggca atatcgcaat ggctcaaagt ttttacattg agtttctatc gccagctgag
    16261 atcagagggc agcgtgttaa ttttgtggca ggcgcatcaa cagttgtatg ccacttacca
    16321 tttggtgatt cattgaacac tcagttgacg gacccaattc ttgcgggaga tccaaatgat
    16381 ccgaacaaca ctcaatttac atttgcggaa gctctgaacg gcaatgttgg tgtatgcaag
    16441 agaggaagtt tttcttttaa cgtaaaggcc tctaatattg aattcaatgg tggtagcgga
    16501 tccattataa tcaacaatga tcaggctaat aaggatgcta tcatatcaat gtcacaggac
    16561 acttccttta gtatttggag tgttgctgtt tcatttaatt caggtgagaa tttcttcctg
    16621 aaatatcccg gcctgagctt taagtttgtc aatgatttcc ctgcagaaac tgacaatatt
    16681 ctctggtcag ataatggatt cagcggaggt ctggatggct ggaccactgt tggtgtcgct
    16741 tgtggagacg gcaaggatcc tgtgggcgct gagtgggaat ggactgaatt tggctcttca
    16801 aaaggtgctt atggtagcac aaacattctt tctgcgacag catgtaatgg tgccatgacc
    16861 tttaatactg actttaagga taatgatggt gttcaaggta attttggcgg tggtatatgt
    16921 ggtgtcaatc aaataggtga gcttatttct ccggacattg accttagtgc cttaccgga
//
LOCUS       DAOQMC010000018        16793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1230_length_16793_cov_5.386605, whole genome shotgun sequence.
ACCESSION   DAOQMC010000018 DAOQMC010000000
VERSION     DAOQMC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16793
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1230_length_16793_cov_5.386605"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKV31_01460"
     CDS             <1..278
                     /locus_tag="PKV31_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNB91546.1"
                     /translation="CHAEIEAIRVAVNFLHTNDLSDCILYTSHEPCIMCSYVIRFHRI
                     RKVVFLHKVEYLGGYSSSMALLTGMDVPPSWSKPPEVVCLDNTEEEQ"
     gene            complement(371..1759)
                     /locus_tag="PKV31_01465"
     CDS             complement(371..1759)
                     /locus_tag="PKV31_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HNB91547.1"
                     /translation="MKYFFLFATLIYSFTLSAQEYDRLNIDLLGRWSVPTEKEEEFGT
                     RYSSCWAFEYNGREYAIVGCGNGTAYIDITDPKNPVFIDFVPSNIKDAIWREYKTYKH
                     YAYHVSDDGGPNTFDIVDFSYLPDSVHVVYSGSKEYFRTGHTIFMDSDKMYIGLHNHR
                     SMSVFSMENPVDPKFLRLLSSDYPGDVHDMFVRNDTVYASKGFEGALQIMTFKDDKFT
                     QIGNFTNYPFAGYNHSSWLTPDGKTLIFTDEVPKSLPAKSLDVSDPSNPILLDTFYSQ
                     SGRATLHNPFESPNNTFIMTSYKDGVQVFDYSDPGDIKLRGYFDCYPQQDGEATDGDY
                     DGAWSGYAMLPSKNMIVVDISNGLFVLDATKAYYPETNTVKAATKAIKLETYPNPSTD
                     AFYLKVPEGLKGPMEVRIVTLSGQLVYQNILEASLMAKHRFNTNTYSPGTYIITLKHG
                     KDIYSSEQIIVK"
     gene            complement(2178..3272)
                     /locus_tag="PKV31_01470"
     CDS             complement(2178..3272)
                     /locus_tag="PKV31_01470"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNB91548.1"
                     /translation="MYDIILSFITAFLLTYFAIPSIINIAKVKKLVDLPDERRSHDEA
                     TPSLGGIGIFGGLLFSVIMWTPFNVFGDLQYVLCSLVIIFLIGAKDDILPMSPNKKIL
                     GEIFAAVILVFKANVKITSLYGIFNIYDLHPVASALFSIFSILVIINAFNLIDGINGL
                     SGSIGTLIAVTFGTWFFLVQRIELSLVAFALAGSLIAFLKYNYTPAKIFMGDTGALIV
                     GLVCAVLSIKFIEFHKEAPESIYAFHAAPAVAIGIMIFPLYDTLRVFVLRVANKKSPF
                     YPDRKHIHHMLIDLGLTHMQATLILVTINALFIVFSFIFQDIGSFNLIMILLGMALTG
                     SIILKQLLNYKQKELRTSKSKSDKTLAGIK"
     gene            3648..5030
                     /locus_tag="PKV31_01475"
     CDS             3648..5030
                     /locus_tag="PKV31_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNB91549.1"
                     /translation="MICTDGGGKYRKRVKLTSLLQAGHYFLMDFHWSVKNRDAQNCLQ
                     TRRSFLGYSHQLWPAVRLWHASIFQYHTFSFSRRVTCIHKILFIIFHLAKFQLIMTDY
                     QNPNLVVHPGKVRSGKITWRSPSNIALIKYWGKYGNQLPRNPSISFTLDAAYTETILE
                     YGSKVSDKPGVSVDFMFHREHNLQFSEKIERFLTSLLPIFPFLSQIDLTIFSSNSFPH
                     SAGIASSASAMSALALCLCSLEDELFGTLSDDAEFEQKASYIARLGSGSACRSIFSKA
                     ALWGETPDVKASSNLYATGWGHHLHPIFTTFHDDILIISKDEKSVSSTAGHKLMENNP
                     YAETRYMTVDTRLKRLITALKSGDVERFGAIVEQEAFGLHGLMMLSEPPYLLMQPSTV
                     AAIQRLMSWRSSTKIPLYFTLDAGPNLHLLYPDESASKVAAFIRSELLPFCVEGSFIP
                     DRVGNGPRQL"
     gene            5125..5694
                     /locus_tag="PKV31_01480"
     CDS             5125..5694
                     /locus_tag="PKV31_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNB91550.1"
                     /translation="MVIDIILVVLIALAFYAGYNNGIFGISLKIVLFFGAILLALNLF
                     PIVFLFMENTFTTLTPVHYILGFVLVLATIFVLYRYISKKIESWVKANSMRLITRISG
                     GIIISLFVLLLFSFISGRLERWKIVKHEAFENSILHPAVDRIDQSFSALIRATGDIFG
                     KTFERNIKTINDIDSHKKEDPEKADTTKE"
     gene            complement(5827..6180)
                     /locus_tag="PKV31_01485"
     CDS             complement(5827..6180)
                     /locus_tag="PKV31_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91551.1"
                     /translation="MKAQLKEMNPLSKIKIEIQNTKKGVTLKAMEGCAWSVLGFNLTE
                     GQSQNIDNFGMVGQNDPLAVNTSSHSDFLITITKTREGFRLDGIKGTSWVALTFRLAV
                     GKKSTFDDTGVWSED"
     gene            7336..9537
                     /locus_tag="PKV31_01490"
     CDS             7336..9537
                     /locus_tag="PKV31_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNB91552.1"
                     /translation="MRIILLLSFFFVFTRLDSQEVKSPSGQVTLTFQLIESVPTYRLN
                     YKDKEVIRTSRLGFELMDKGSAKNSFADEVIKDNSSDNRLDNLKDGFEVVGTTTSSFD
                     ETWQPVWGEERNIRNHYNELVINLIQTSTRRLMTLRFRVFDDGIGLRYEFPEQKNLIY
                     FTIREEKTQFVLAGDHTAFWIPGDYDTQEYDYNTSRLSEIRSLMSTAITPNASQTPFS
                     PTGVQTPLMLKTDDGLYINIHEAALINYGAMHLNLDDKQNILESWITPDANGSKGHLQ
                     APCHTPWRTVIVSDDARDILASRITLNLNEPCKIEDVSWIHPVKYIGVWWEMITGRSS
                     WAYTDDVPSVILGKTDYTKTRPNGRHAANTAHVKEYIDFAAEHGFDAVLVEGWNTGWE
                     DWFGNSKDHVFDFVTPYPDFDMAAIRDYARQKGVKMIMHHETSSSVRNYERYLDTAYQ
                     YMKTNGYDAVKSGYVGDIIPRGEYHYSQWINNHYQYALEKAAEYRIMVNAHEAVRPTG
                     ICRTWPNLIGNESARGTEYQAFGGSKPNHVTILPFTRLIGGPMDYTPGIFEMDISRIN
                     PNNHSHVNSTLANQLALYVTMYSPLQMAADLPEHYRQYPDAFRFIKDVAVDWDVSRYL
                     EAEPGEYITIARKAKNSDNWFVGGVAGNNGHTSLLQLNFLDADKVYIATLYMDGPDAD
                     YKTKPQSYIIKSGVVDQATKLAIKSAPAGGFALSLIAVEKKAVKGMRRLKN"
     gene            9991..10536
                     /locus_tag="PKV31_01495"
     CDS             9991..10536
                     /locus_tag="PKV31_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91553.1"
                     /translation="MKVHTIFLIISLLHAVNSYAQWPSNVMVLDPGEKSSIEVQGDLA
                     TGKVMEDLSWAWNSSNACFPETQASKFRGNHVFYAITMPTHCEMTISVVPTDNKGDLS
                     IYAYRIGASAYDLVPDLSRCITCEADYKWDKNWKGKVQTSERRVSMQNPSSNNYNILI
                     GVAGPASVTKGIFTLSVQFTR"
     gene            10936..11433
                     /locus_tag="PKV31_01500"
     CDS             10936..11433
                     /locus_tag="PKV31_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91554.1"
                     /translation="MKRCTTLLLLLGFLIPGTMFSQDLAQNNEYKTFASSAHLDYMDQ
                     ATESDLYLYSGNLMEKDKCRTYTKMKKAGLVMTIVGPIVMVGGVAMMLTGILTSDEEL
                     DVNTGLVVGGYVGIVAGALTTAGGIPLLIVGNVKSKQYCNGKKRNSQLNLNSGKNGVG
                     VALAF"
     gene            11582..12076
                     /locus_tag="PKV31_01505"
     CDS             11582..12076
                     /locus_tag="PKV31_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91555.1"
                     /translation="MRTIMNMLLVLGVLIPFAMNGQEMASNSGYKTFASSVGIEFIDR
                     ATDSDLNLYAGKIQYKDKCTAFTKMKKAGTFMTVFGSIALISGFIASNSDADFQNGGY
                     FFSDADIISFLGFTSGVLLTGAGIPLMIVGIVKSKNYCGSNGSTPRIILKSEGNSAGL
                     ALIF"
     gene            complement(12188..12964)
                     /locus_tag="PKV31_01510"
     CDS             complement(12188..12964)
                     /locus_tag="PKV31_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91556.1"
                     /translation="MMWEDIKRVKINEIQQKEKDTPLDLLEELALTAPDSVSLAEALI
                     NPLKSHTIAEFKRKSPIDGEMAPNADAMEVVSQFLQSGACAISIATDGSFYGGNIDDL
                     HLTKQIFREVPVIMREYIVDPYQIVEARALGSDAIILHGGLLTAEEVASFARLAHALE
                     MEVVFESSNEKDLKDYHPDVDIIMVHHRYNHEDVATPSKILSYLSQLPADKVRVGGGG
                     LKSYNQRKMLLDAGFDAVIIGTSLMKSKNHGDTLHSIIVG"
     gene            13385..14038
                     /locus_tag="PKV31_01515"
     CDS             13385..14038
                     /locus_tag="PKV31_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91557.1"
                     /translation="MSILILGLLALLGYSLFAKGYFNFQPEGRRYEKISLEVQDYLKA
                     QQPGLVNFQEDELGLLSGNKQKSGKSGFFGKQTEGVLLSIYHEPFIAYKATSLGQDKE
                     TGVIGIATFKDQYLYIIKKDHTDIFINKKPIGTIKNGGQLVLLNSKSIAATYEIKNNE
                     SWLVRMNSAPEAEVRLRIVENPLPQRVLDIYESNSGINKTILRAVIFYLTGLGLRQL"
     gene            14143..15870
                     /locus_tag="PKV31_01520"
     CDS             14143..15870
                     /locus_tag="PKV31_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB91558.1"
                     /translation="MKPGRLIITGGLVLCLFLFDSCRKEPRVALDTVNVAIAESPETL
                     FPYKAKSVVAGQVISKIYLPLADFNPMTLELEPVLLESIPKPEIDSTGERYHFRILKE
                     ASWSDGRPVTVSDVLYTMMFIVNPFGNHGSVRSTLANIDDLSFQDPTSKEFSVTFNGE
                     YHLDLEAFTNIPLLPAHVMDPDHLTAAYTWRQMTVGIDSLRMGQDSTTLIRIAGQSKL
                     LERDFTKQVGTGPYKVSEWVPNQRLTLVRIEQYWGMPLAETYTEFQAFPATINYLIFP
                     EVSTAMAALKAGQVNVMPEVNEEILDQVRSDATLAKNFDVLSADVLQYYYIGLNNAGP
                     MLGDLNVRRAMNHLVNVDRIIQTLMHGNAEKVKSPILAQKPYYVSNLPDIEYNPAKAR
                     AILDQFGYHEIGADGIRTKKINGQPMRMSFTILTTGKQLGRDVATLLINEARKVGIEV
                     KMETMDFPKILERIKSGKYDMANLVVKQFPGLDDPYLAWNSTNAFGKGSNYCNVNNPA
                     IDRITEEIRTAPSQADRYKLYREFQVKLAEQYPVIFLFSPKNQILIQEGLTMVTSPKR
                     PGYFENTVK"
     gene            15901..>16793
                     /locus_tag="PKV31_01525"
     CDS             15901..>16793
                     /locus_tag="PKV31_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91559.1"
                     /translation="MTTGIIYNLLRIFAGLLKLMIKVLVIVLSVYFLASIIGSRNIED
                     GDTYQGDEFKTHLPAFYFSVLPGWYDARLSLYPAGPVKKQGIDFLKNGLTYNDWLKFR
                     ALVDTISGRPQYKNWNELKYIHLISEINSRIRMESKNAAPDEFLQKNLAEISGLLTGR
                     HRITGIKYLPQLQFWGKRNNFHQELMSVFSTTDKWLQLRSAFNLSFVLSLLAYLSTIL
                     FGTLLALWYMRMKSLGRRILMTYLNIIYVFPVFCLTVIAVQFFTSDYYSPWLNWFPGP
                     GTFLMMSSVSLGSVFSDSVGY"
BASE COUNT         4954 a         3371 c         3563 g         4905 t
ORIGIN      
        1 cttgccacgc cgagatagag gcaatcaggg ttgcagtaaa ttttctgcat accaacgacc
       61 tgtcagactg tatcctttat acgtcccatg agccatgcat catgtgttcg tatgtcattc
      121 gtttccatag aatcaggaag gttgtctttt tacataaggt cgaatatctt ggaggatatt
      181 cctcgtcaat ggctttgttg actggtatgg atgttcctcc atcatggagc aagccaccgg
      241 aagtagtctg cttggataac acagaggaag aacaataaat atcctatgca tgtggggata
      301 ccgaacgtct ctgtgcatat caaaagagaa gttcagtacc atgctatcgt tggatatttt
      361 ctgaaaagat ttacttcacg ataatctgtt ctgaagaata gatatcttta ccgtgtttca
      421 atgtaattat ataggttcca ggagaatagg tattggtatt gaaccgatgt ttagccatca
      481 aggatgcctc aaggatgttt tgatatacca gttggccgga caaagtgacg atccgcactt
      541 ccattggtcc tttcaagcct tccggcactt tcagataaaa agcatcggtt gaaggattgg
      601 gataggtctc gagtttaatg gctttggtcg cagccttgac tgtattggtc tccggataat
      661 aggctttggt ggcatctaat acaaataagc cattgctgat gtccacaaca atcatatttt
      721 tactcggcaa cattgcatag ccgctccagg caccatcata atcgccatcc gtagcttcac
      781 catcctgttg cggatagcag tcaaaatagc ctctcagctt gatatcgccc ggatccgaat
      841 aatcaaatac ctgaacacca tccttgtagc ttgtcatgat gaatgtgttg ttgggagatt
      901 caaatggatt atgcaaggtg gctctccctg actgagaata aaaggtgtcc agcaggatgg
      961 gattggaagg atccgataca tccaaagact ttgcaggaag gctttttggt acctcatcgg
     1021 taaatatcaa ggtctttcca tcaggtgtca accaggaact gtgattatac cctgcgaatg
     1081 gataattggt aaaatttcct atctgagtaa atttatcatc cttaaatgtc atgatctgca
     1141 gagccccttc gaaacctttc gatgcataca cggtatcatt ccttacaaac atatcgtgaa
     1201 catctccggg ataatcactg gacaaaagtc gtaaaaattt agggtcgacc ggattttcca
     1261 tgctgaatac cgacatggaa cggtgattgt gtaacccgat gtacattttg tcgctatcca
     1321 tgaatatcgt atgtcctgtc ctgaaatatt ccttactacc cgaatatacg acatgtacac
     1381 tatcaggcag gtaagaaaaa tcaacgatat caaatgtatt aggtccgcca tcgtcgctta
     1441 catgataggc gtaatgctta taggtcttat attcccgcca aatggcatct ttgatgttgc
     1501 ttggtacaaa atcaataaaa acgggattct tcgggtcagt aatatcaatg tatgccgtcc
     1561 cgttgccaca gcctacaatc gcatattccc tgccattgta ttcaaaagcc cagcaggaac
     1621 tataacgggt accgaattct tcttcttttt cagtaggaac cgaccaacgc cccagcaaat
     1681 caatgttgag cctgtcatat tcctgtgcag aaagggtgaa agagtatata agggtagcaa
     1741 aaagaaaaaa atatttcatt tctataaaaa ttaaatttag tgcgccaaat gtaagccttt
     1801 tatatctttt aatcggaagc cttgtcgtga aattttgtat tgtaaatctt aatgatatta
     1861 taattctcaa tttcaggaca tcaccgggat tttttgtttg gccatcaatc atgttgtatc
     1921 tgtttccatc aaaccgatgt cattccctct ttgagtttcc agagtctttg agcgcgaaga
     1981 aatgttgttc gttatcggta agtaaagagt gaaaagagaa tagtgcgggc acatataagt
     2041 ccggagtgac aaaggattca caggtaaaag tgctaaaaat tcattattaa agacaaatat
     2101 attggttgat caactacaag ttgtgatgtg gttgcctttc acaaaaaaag attctgaagg
     2161 tggctgtatc acatttctta tttaattccg gccagcgtct tatcggattt tgatttactt
     2221 gtccgtaact ccttttgttt gtaatttaat aattgcttca ggataatact tcccgtaagc
     2281 gccatcccca aaaggatcat gatcaggttg aaggagccta tatcctgaaa aataaatgaa
     2341 aatactatga acaaggcgtt gatggtcacc agaataaggg ttgcctgcat atgggtcagg
     2401 cccaggtcaa tgagcatatg gtgtatatgc ttccggtccg gataaaaggg acttttctta
     2461 ttagctacac gcaggacaaa tactctcagg gtatcgtaca atggaaatat catgatgcct
     2521 atggcaactg caggtgcagc atggaatgcg taaatcgatt ccggcgcctc cttgtgaaat
     2581 tcgatgaatt taatggatag gacagcacaa accagcccca caatcaatgc cccagtgtct
     2641 cccataaaga tttttgctgg tgtataattg tactttagga aagcgattag agaaccggct
     2701 aatgcaaaag ctaccagact gagctctata cgctggacaa ggaagaacca tgtgccgaaa
     2761 gttacggcaa tcagagtccc tatacttcca ctcaacccat tgatgccgtc gataaggtta
     2821 aaggcattga tgattaccag aatggagaat atcgaaaaca atgcggaagc tacaggatgc
     2881 agatcataga tattgaatat tccatataag gaagttattt ttacattggc cttaaatacc
     2941 agaatcacag ccgcaaatat ctcacccagg atctttttat ttggtgacat cggaaggata
     3001 tcatctttgg ctccaataag aaaaataatc accagcgaac aaaggacata ctgaaggtct
     3061 ccgaatacat tgaaaggtgt ccacataatg accgaaaaca acaaaccacc gaaaatgcca
     3121 attcctccaa gggaaggcgt tgcctcgtca tgggagcggc gctcatccgg taagtccaca
     3181 agttttttga ccttcgctat attgatgata gaaggtattg caaagtaggt cagcaaaaat
     3241 gccgtaataa aagataatat tatgtcgtac ataggttcgg attttacaaa tttgcatttc
     3301 ttctttttgc agaatgacgc taaattgtat tccgagggca aatttaatac aattaccgac
     3361 aggatgagcc tgttttctta tatttggaat aattctatgc ttaaaattgt gttgataatg
     3421 atcactgatt ggcaatcgac ttcttatttc ttattaccct tatgcttatc ctgtaaatgt
     3481 gttatctaac taataacgaa gcggtgtttg aagatgacca tcagacgtgt tcagtaatgg
     3541 caatgaaaga caaatctccc gggatgtaag cacctataat agcttgcagt gtattcacct
     3601 cacctctatc aaaacgcagt aaaatccggt aaaattttgc cgaaataatg atttgcacgg
     3661 atgggggggg taaatatagg aagcgtgtta aactgacttc cctccttcag gcaggccatt
     3721 attttttgat ggacttccat tggagtgtga agaacagaga cgcccaaaac tgtttgcaga
     3781 cacggagatc ctttctgggc tatagtcatc aactttggcc tgcagtaagg ctgtggcatg
     3841 catcaatctt tcaatatcac actttctctt tttcacgacg cgttacttgc atacataaga
     3901 ttttatttat tatctttcac cttgcaaagt tccagttgat catgaccgac tatcaaaatc
     3961 cgaatcttgt cgtccatccc ggtaaagtgc gctccggaaa aataacctgg cgcagtccgt
     4021 ccaatattgc gctgatcaaa tattggggaa aatacggaaa tcaactgccg agaaatccat
     4081 cgatcagctt tacattggat gcagcctata cagagactat tcttgaatat ggatccaagg
     4141 tgtccgataa gcccggagtt tccgtcgatt ttatgttcca tcgtgaacac aatctgcaat
     4201 tttcagaaaa gatcgaaagg ttcctaacgt ccctgttgcc tatttttcca tttttaagcc
     4261 agatcgacct taccatattt tcatccaaca gctttccgca ttcggcaggt attgcctctt
     4321 ctgcttcggc catgagcgca ttggcgctat gtctgtgcag cctagaagat gagttgtttg
     4381 gcaccttgtc cgacgacgcc gagtttgaac aaaaagcatc atatattgcc agactgggct
     4441 cagggagtgc atgcagatcc atattctcaa aggctgcact gtggggtgag actccggatg
     4501 tgaaggcatc ttccaatcta tatgccacag gctggggaca tcatctgcat cccatcttca
     4561 ctacctttca tgatgatatc ctaatcatta gcaaggatga aaaaagtgtt tcatcgaccg
     4621 ccggacacaa actgatggaa aacaatcctt acgcggagac gcgttatatg accgttgata
     4681 ccagactcaa aagattgatt acggccttga aaagcggcga tgtagaacga ttcggtgcca
     4741 ttgtagaaca agaggccttc ggtttgcatg gactcatgat gttgtccgaa ccaccatatc
     4801 ttcttatgca gccttcgacg gttgcggcca ttcaaagact tatgtcatgg agatcatcga
     4861 caaagattcc attatatttt accctcgatg ccggacccaa tcttcacctc ctatatcctg
     4921 atgaatctgc ctccaaagtc gcagccttta tcaggagcga attattgccc ttctgtgtag
     4981 agggctcatt tataccggac agggtaggca atggcccccg gcagttgtaa tatgtacaga
     5041 ctataaaatt caaggcaata aaaacaagca tttgatatcg gctattatgc ctatctttca
     5101 cctgtaaaac aactaaatcc aggaatggta atcgatataa tccttgttgt tctgattgca
     5161 ttggcattct atgccgggta taacaatggc attttcggta tttccctgaa gattgttctg
     5221 tttttcggtg ccatcctctt ggccctcaat ctttttccca ttgtgtttct ctttatggaa
     5281 aacacattca ctaccctcac tccggtacat tacattctgg gatttgtctt ggttttggcg
     5341 acaatctttg ttttataccg ttatatttca aagaaaattg aatcctgggt caaagccaat
     5401 tctatgagat tgattaccag aatttccgga ggcatcatca tatccctgtt tgtactctta
     5461 cttttcagct ttatttccgg cagactcgag cgatggaaaa tcgtaaagca tgaagcattt
     5521 gaaaattcta tattgcaccc cgcagttgat cgcattgatc aaagtttttc tgcattaatc
     5581 agggccaccg gcgatatttt tggaaaaaca tttgaaagaa acatcaagac catcaacgat
     5641 atagacagcc acaaaaagga agatcctgaa aaggccgaca cgacgaagga ataacagcag
     5701 cagggtcaca catcgcgctc tctctcagga aatttttgat acaaaaacag gaatcggtat
     5761 gtcttggccc ttaaaagccg ggtcgatacc ggaaagatta tttcaccatg attttacgct
     5821 gtcgtctcag tcttctgacc aaactccggt atcgtcaaag gttgattttt ttccgacagc
     5881 aaggcggaat gtcagtgcaa cccaggatgt tcctttgatg ccatccagcc tgaagccctc
     5941 tctggtctta gtaatggtga tcaaaaagtc agaatgactg gatgtattga ccgccaaggg
     6001 atcgttttga ccaaccatgc caaaattatc aatattttga gattgccctt cggttagatt
     6061 gaaccccagt acagaccatg cacagccctc catggccttc agagttacgc ctttcttggt
     6121 attctgaatt tcaattttga tttttgacaa tggattcatc tctttgagtt gtgccttcac
     6181 tgaacaactt attgtaaaaa gaaataaaac caaaaatcct gctattaatt taccattatt
     6241 gtgataaaac ctcattttca taagttttat gataaagata agctatccac atgaatattt
     6301 gctttattag tggatgaaaa agcattccat ctcaataccg gaatatcagg tattgagacc
     6361 taaggaccac catttctctt attataaatt tatttagaag taacaggcat gaggtgccgg
     6421 tgaatgacaa tattcttcta actaaccttt cctctatgtg cctttgcaaa caaataaagt
     6481 ttcggaggta tgtacatcta caccatgtag acatattgtt gtttgccatt tataaatgtc
     6541 ataggacaag gaaaacctca tcttataagg ataatatgga tcaactttta atctgttaaa
     6601 gactatccaa taaagggtgt cttcaatttt acacctcatc atcgtttgga atttattttt
     6661 cataatgtat cctttatttc atcagcctat tgtttttctt cgttttataa atattgacct
     6721 aaaggagctg tcatactttt tacgtatatt ttaaaatgat tagcgcccaa tttattttga
     6781 tttggaattg ccgggtaatt aatatgaatt tgtgccgggg ccagtaatct cgctttattg
     6841 aaagctaaaa accggaagtg tcttttttta aaattcttat tagaaaccta atatcaataa
     6901 gggtttgaat gaggtggaaa atatggttgc cattgaggtt aagtaattat gcttcaatgc
     6961 gataatctcc ctgatcgata aggttattta actgaagaag ccgatctcct tccaacatat
     7021 aattgtatag tataatgcac ttatactgaa atcaagatgc ctaatggaaa tctgtatttt
     7081 gtaggaaaaa ctttaagatc atttttctca aatcctcacc caatcgttta ccttggtgaa
     7141 atcaatactc cgctaaaacc ttacagtaag gcagcttacc atgaaatcat atcatttaat
     7201 aggaaaaaat ctgatcgggc ttcaaaaacc catggatatg caagagtatt caacagagga
     7261 aacctccata atgggtgcaa atgcgttgaa ttccattttt tttgcttaaa ctcgcactcg
     7321 taaaattatt tcatcatgcg gatcatttta ttgctgagct tcttctttgt ctttacccgg
     7381 ttagatagtc aggaagtaaa atcaccttcc ggacaggtta cattgacctt tcaattgatt
     7441 gaatcggttc cgacttatcg ccttaattac aaggataagg aagtaatcag gaccagtagg
     7501 ctggggtttg aactgatgga caagggatct gcaaagaata gctttgcaga tgaggtaata
     7561 aaggacaaca gtagtgacaa ccggctggat aatttgaagg atggttttga ggtagtgggc
     7621 actaccacat catccttcga tgaaacctgg cagccggtat ggggagaaga gcggaatatc
     7681 aggaatcatt acaatgaact agtcatcaac ctcatacaga catcgaccag gaggttgatg
     7741 acgttgcgat ttcgagtgtt tgatgacggt attggcctac gatatgaatt tccggaacaa
     7801 aaaaacctga tttattttac catcagggaa gaaaagacac aatttgtcct tgccggggat
     7861 catactgcct tttggattcc gggagattat gacactcagg aatacgatta caatacttca
     7921 agactttcag aaatccgatc attgatgtcc acggctatca cgccaaatgc ttctcagact
     7981 ccattttcgc ccaccggtgt acagacacct cttatgctta agaccgatga cgggctttac
     8041 atcaatatcc atgaagccgc attaatcaat tacggtgcta tgcacctcaa tctggatgat
     8101 aagcaaaaca tcctggagtc atggatcact cctgatgcca atggcagtaa gggtcatctc
     8161 caggcaccat gccacacacc atggcgtacc gtgatagtca gtgatgatgc acgggacata
     8221 cttgcctccc gtattacgct caatcttaat gagccttgta agatagagga tgtctcctgg
     8281 atacatcccg ttaagtacat cggtgtctgg tgggagatga ttaccggtcg gagttcatgg
     8341 gcctacacgg atgatgtgcc cagtgtcatt ctcggtaaga cggattacac caagacacgg
     8401 cccaatgggc gccatgctgc aaatactgcc catgtcaagg aatacataga ttttgctgca
     8461 gaacatggat ttgatgctgt gttggtcgaa ggctggaaca caggatggga ggattggttt
     8521 ggcaattcaa aggatcacgt cttcgatttc gtaacgccct atccggattt cgatatggct
     8581 gcgatacgcg attatgccag gcagaagggt gtgaaaatga taatgcacca cgaaacctct
     8641 tcctctgtac gcaattatga acgctacctg gatactgcct atcagtatat gaagaccaac
     8701 ggttatgatg cagtcaaaag cggatatgtg ggcgatatca tacctcgtgg cgaatatcat
     8761 tacagccaat ggatcaacaa tcactatcaa tatgctctgg aaaaggctgc agaataccga
     8821 atcatggtca atgcccacga agcggtccgg cctactggga tctgccgtac ctggcccaat
     8881 cttattggta atgaatcggc ccgtggtacc gaataccagg cattcggggg cagtaagccc
     8941 aatcatgtca ccatattgcc ctttactcgt ctgatcggcg gtccgatgga ttatacaccc
     9001 ggcatctttg aaatggacat ctccagaata aatccaaaca atcactccca tgttaactcc
     9061 acgctggcca atcaacttgc cttatatgta accatgtata gccccctaca gatggcagcc
     9121 gacctgcctg aacactacag gcagtatccg gacgcattcc gctttatcaa ggatgtggca
     9181 gtggattggg acgtaagccg gtatctcgaa gcagaacccg gcgagtacat caccatcgca
     9241 cgtaaggcga agaacagtga caactggttt gtaggaggcg tagcgggcaa caacggtcac
     9301 acctccctgc ttcaactaaa cttcctggac gcagacaagg tgtacatagc cacactctac
     9361 atggatggtc cggatgcaga ttataagacc aaaccacagt cgtatatcat taagagtgga
     9421 gttgtagacc aggctacaaa gttggcaatc aaatcagccc ctgccggggg ctttgctctt
     9481 tccctgatcg cagtagaaaa aaaggcagtg aaagggatga ggaggttgaa gaattgatcg
     9541 gtggtgtata agccggaaag ggtattgcag gtcaataaga attaacattt atcacctgta
     9601 gccaatgctt caagacgaca aaaatggtcc ataggctatt ccgtaatagt tattctatat
     9661 ggttgaggga gttccggcca caattcctaa ggatggcagg ttattctaac aatggaaggt
     9721 gagaactgaa atggctatga aatggaattg ctgcagaaat tctaatattc ttttttagat
     9781 caatttccgg attttactta taccttttga tttgtcagga aagatttccg gagtttttga
     9841 cttttgtttc aagaaataag ataaatttgt agtactgtat ttagcatgga tatacccttt
     9901 aagagatgga aaggtattta attgaaaatg gatttccatt ttttacccgt tgtaaatgat
     9961 tacctgtttt aatttattaa attgttattt atgaaagtac ataccatatt tttgataatt
    10021 tcattacttc atgctgttaa ttcctatgcg cagtggccat caaatgtgat ggtattggac
    10081 ccgggggaga aaagtagcat agaggtccag ggtgatttag ctaccggaaa ggtcatggaa
    10141 gacctcagct gggcttggaa tagctcaaat gcctgctttc cggaaacaca agcctcaaaa
    10201 ttcagaggaa accatgtgtt ttatgcaatt actatgccga cacattgtga gatgaccatc
    10261 agtgtcgtcc caaccgataa taaaggagac ttgagtatct atgcatatcg tatcggagca
    10321 tccgcctatg atttagtgcc agacttaagt cgctgtataa cctgcgaagc tgattataag
    10381 tgggataaaa actggaaagg aaaggttcag accagtgaaa gaagggtcag tatgcaaaac
    10441 ccaagctcaa acaattacaa tatattgatt ggagtggcag gtcctgcctc agtaaccaaa
    10501 ggaattttta ccttaagcgt gcaatttacg cggtaggttt tttcttatgt caattaaaaa
    10561 actccttgtt ttatacctag gtggcggcaa tgcagaggct gcacccgaaa ggaatggttg
    10621 catagtgggt tgttgtattt aaaagacgat ttttttagtt caaataaagg ttgtcggtat
    10681 agatacatca aacaaatatt ataagaaaat tgacatttct taccttcaaa atcctaatag
    10741 cgttgagctt ttcggcacaa aatagattgc gtgaaacagc gtaatcgaat agttgctaag
    10801 gtagcattgg taataaattg tcgttgattg aatttattgc aaaagagaac agcattaaat
    10861 tgatctgaaa agttacggaa attttgtaat taaggttttt atattaaatt tacccaaaat
    10921 aaaaacaata taatcatgaa aagatgtaca acattactat tactcttggg ttttttgatt
    10981 ccgggtacca tgtttagtca ggatttggca cagaacaacg aatataagac atttgcctca
    11041 tctgcccatc tggactacat ggatcaggca accgaatcag acctgtattt atactcggga
    11101 aatctaatgg aaaaggacaa atgtaggact tataccaaga tgaaaaaagc cggtttagtg
    11161 atgaccatag taggaccaat agtcatggtg ggcggcgttg caatgatgtt gaccggaata
    11221 ttgacaagtg atgaagagtt ggatgttaac acaggactcg ttgtgggtgg atatgtcggg
    11281 atagttgccg gagctttgac aactgccggt ggaattccac tcttaattgt gggcaatgta
    11341 aaatcaaaac aatattgtaa tgggaagaaa cgcaattctc agttgaacct taatagcgga
    11401 aagaacggag tcggtgtggc tcttgctttt tgattaatga aaaaaatatt cgacataaag
    11461 agggatgcta acattattat gcgtcccttt ttttgtattg gcaatctaat aacctcccct
    11521 gatttgaatt gaatcagggt ttattttttt atctttaagt tgaaaattaa gccaaaataa
    11581 tatgagaaca ataatgaaca tgcttttagt tttaggcgta ttgattccat ttgcaatgaa
    11641 tggacaggag atggcgagca attccgggta taaaacattt gcatcttctg ttggcattga
    11701 atttattgac cgggcaacag actcggatct aaacctatat gccgggaaaa tacaatataa
    11761 agacaaatgc acggctttta ccaagatgaa aaaagcggga acttttatga cagtatttgg
    11821 ttcgattgct cttatttccg gttttattgc gtccaacagt gatgcagatt tccaaaatgg
    11881 cggttatttt ttttcagatg ccgatataat tagttttttg ggctttactt ccggcgtctt
    11941 gttgacagga gccgggattc cgcttatgat tgtcggcatc gttaaatcaa aaaattattg
    12001 cggatcaaac ggatcaacac caagaataat tttaaaaagc gaaggaaata gtgccggctt
    12061 agcgctgatc ttctgatatt taaccgtaca agagacaata tgcattcgag aaggaagagt
    12121 gccctgcagg atgatttgac aactggcaat ctatggatta accactacgg ggccaatttt
    12181 tcatcaatta tccaacaata atcgaatgca gtgtatcgcc atgatttttc gatttcatga
    12241 gggatgtccc gatgattact gcgtcaaaac ctgcatcgag caacatcttt ctttgattat
    12301 aactctttag tccgccaccg ccgaccctga ctttatcagc aggaagttgg gacaggtacg
    12361 ataagatttt tgaaggagtt gcgacgtcct catggttata tctgtggtgc accatgatga
    12421 tgtccacatc cggatgataa tctttcaaat ccttttcgtt ggaagactca aagacgactt
    12481 ccatctccag tgcatgagcg agacgggcaa aggatgcaac ttcttcagca gttagcaagc
    12541 ccccatggag gataatagca tcgcttccta atgctctggc ttccacgatt tggtaaggat
    12601 ccacaatata ttcacgcatt attacgggaa cctcccggaa tatttgtttg gtcaagtgca
    12661 ggtcatctat gttgccgcca tagaagctcc catctgttgc tatgctgatg gcacatgcgc
    12721 cgctttggag gaattgagat acaacttcca tcgcatcggc attcggagcc atttcaccat
    12781 ctatgggaga tttgcgcttg aattccgcaa tggtatgtga cttgagggga ttgatgaggg
    12841 cttctgccag ggaaactgag tcaggagccg tcaatgcaag ctcttccagg aggtcgagtg
    12901 gagtgtcctt ttctttttgc tgtatttcgt tgattttgac tcgtttaata tcttcccaca
    12961 tcataaatcc tggcttgatg cctatatatg gctctctttg tatttttttc ccgtttgata
    13021 tgctgatcat tggtaacgca caaaggtact cagccggaag ataatttttg gaacgattct
    13081 catcattaaa aaggattaaa tagaatcaaa ttgtcctacc cacacccttt gcaatccgag
    13141 aaaagctcaa ttgaaatcgt atgaatatga gggaagcctt accggatgaa ataatcagta
    13201 ggataaagag caatgctata acataacttg acctaccagg agtgactggg tgcttagttg
    13261 ttctacattt agctttaatg aaatgaagtc aggtgtttat atattggtaa cggttctttt
    13321 tgtgaaaata ttgaaatggg acacaaaatt ttgatttgtt atatttgcgt aaattgtata
    13381 ctcgatgtct attttaatat taggactttt ggctcttttg ggttattctc tatttgcaaa
    13441 aggatatttt aattttcagc ctgagggaag gcgatatgag aaaataagcc ttgaggtgca
    13501 ggattattta aaggcacaac agccaggtct tgttaatttt caagaagatg aattggggtt
    13561 gttgtctgga aacaagcaga aatcaggtaa atccggtttt tttggaaagc aaacggaagg
    13621 agttctgtta tccatctacc atgaaccttt catcgcctat aaggcaacat cacttggtca
    13681 agataaagaa acaggggtga ttggcatagc taccttcaag gatcaatact tatacatcat
    13741 caagaaggat cacactgata ttttcatcaa caaaaagcca attgggacga ttaaaaatgg
    13801 tggtcaattg gtattactca acagtaaatc cattgctgct acctatgaaa taaagaataa
    13861 tgaatcctgg ttggtgagaa tgaatagcgc ccccgaagct gaagtacggt taagaattgt
    13921 tgaaaatcct ttgccccaac gggtcctgga tatatatgaa tctaactccg gaatcaataa
    13981 aaccatttta cgcgctgtga ttttctatct gacaggtctt gggctgcgtc aattatagaa
    14041 caccgaaaac tataaatatg ggttgcttat ctcgtagata gaaatatttg cctattgctt
    14101 ctgacagaaa attgtaactt caccaaagaa atcaaataat caatgaaacc gggcaggtta
    14161 attattactg gaggtcttgt attatgtctt ttcctgtttg actcttgtcg aaaggagccc
    14221 agggttgcat tagacacggt caatgtagcg attgccgaaa gtcctgaaac cctttttcct
    14281 tacaaggcga agtcggttgt tgctggacag gtaatcagta agatttatct gccacttgct
    14341 gatttcaatc caatgacact ggagttggaa ccggtattgt tggagtcaat tcccaagccg
    14401 gagattgatt caaccggcga aaggtatcat ttcaggattc tgaaagaggc ttcatggagt
    14461 gacggaaggc cggtaaccgt cagtgacgtg ctttatacaa tgatgtttat cgtcaatcct
    14521 tttggcaatc atggctcagt ccgttccacc cttgcaaaca tagatgacct tagctttcag
    14581 gatcctacat cgaaagaatt ttccgtaaca ttcaatggag aatatcatct ggatctggaa
    14641 gcattcacaa atattccctt gctacctgcg catgtcatgg accctgacca cctgacagca
    14701 gcgtacacct ggcgacaaat gactgtcggc atcgattcgt tgcgtatggg tcaagactcg
    14761 acaaccttaa tccgtattgc cggacagtct aaattgctcg aaagggactt taccaagcaa
    14821 gttgggacgg gtccgtataa ggtcagtgaa tgggtgccta atcagcgcct cactttggtt
    14881 agaatcgagc agtactgggg gatgccactc gccgaaacct atacggagtt tcaggcgttt
    14941 ccggctacta ttaattacct catctttccg gaagtatcga cagcaatggc tgcactcaaa
    15001 gcaggacaag tcaatgtaat gcctgaggtt aatgaggaga tcctggatca ggttcggagt
    15061 gacgctacac tggctaaaaa ttttgacgta ctctcagccg atgtattgca atactattac
    15121 atcgggctga acaatgcggg tcctatgctt ggcgatctta atgtaagaag ggcaatgaat
    15181 caccttgtca atgtggatcg gattattcaa accctcatgc atggtaatgc tgaaaaggtt
    15241 aaaagtccca tcctggctca aaaaccttat tatgtgtcca atctgccgga tatcgagtat
    15301 aatcccgcaa aggccagagc tattctggat caatttggat atcatgaaat aggagctgac
    15361 ggcatccgga caaagaaaat caatggccag cctatgagaa tgtcatttac cattctaaca
    15421 acgggaaaac agttgggtag agatgtagca accctgctta tcaacgaggc caggaaggtc
    15481 ggaatagaag taaagatgga aaccatggac tttccaaaaa ttctggaacg aatcaaaagc
    15541 gggaagtatg atatggccaa tcttgtggta aaacagttcc ccgggctcga cgatccatat
    15601 ttggcatgga attctaccaa tgcttttggc aaaggctcca attattgcaa tgtgaataat
    15661 ccggccatcg atcgcattac ggaagaaatc agaacagctc cctcccaggc tgacagatac
    15721 aaactctata gggaatttca ggtaaaactg gctgagcaat atccggttat cttccttttt
    15781 agccctaaga atcaaattct tatccaggaa ggtttgacca tggtaacatc acccaagcga
    15841 ccaggctatt ttgaaaacac ggttaaatag gcttttttga agaaataaaa tcataatgat
    15901 ttgacaaccg gtatcatata taatcttctc aggatttttg ccgggttgtt gaagttgatg
    15961 atcaaggttc tggttattgt attgtcggta tattttctgg cgagtatcat cggcagccga
    16021 aacattgaag atggtgatac ttatcagggg gatgaattta agacacacct tccggcattc
    16081 tatttttcag tattgcccgg atggtatgat gctcgtttga gtctttatcc cgcaggtcca
    16141 gtgaaaaaac agggaattga ttttttgaaa aatggcctta cctataacga ttggttgaaa
    16201 tttcgtgccc tggtcgatac tatatcagga cgtcctcaat ataaaaactg gaatgagctg
    16261 aaatatatac acctcatttc cgaaattaat tcacgcatcc gcatggaaag taaaaatgcg
    16321 gcaccggatg aatttcttca aaaaaatctg gcggaaatat ccggattatt aaccggcaga
    16381 catcgtatta caggtataaa atatttgccg caactacaat tttgggggaa aagaaataat
    16441 tttcatcagg agttgatgtc agttttttcc accaccgaca aatggcttca attgcgatca
    16501 gcctttaatc tttcatttgt tttgagcctt ctcgcctatt tgtccaccat tttattcggg
    16561 accctactgg cactctggta tatgcgtatg aagtccttgg gccggagaat tctaatgacc
    16621 tatctcaata tcatctatgt ttttccggtg ttttgcctca cagtaatcgc tgtacagttt
    16681 tttacttcag attattatag tccatggctg aattggtttc ccggcccggg gaccttcctc
    16741 atgatgagtt cggtgtcttt gggaagtgtt ttttcagact cggtgggcta ttt
//
LOCUS       DAOQMC010000019        16461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1266_length_16461_cov_5.777886, whole genome shotgun sequence.
ACCESSION   DAOQMC010000019 DAOQMC010000000
VERSION     DAOQMC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16461
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1266_length_16461_cov_5.777886"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..487)
                     /locus_tag="PKV31_01530"
     CDS             complement(167..487)
                     /locus_tag="PKV31_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91560.1"
                     /translation="MKQQYTSTNFIRKNTRSLPIGNCYINKTWEVNKLANVIVSRKLK
                     NGNLIVGFYLVDLFCLGLRSSYFQSDMEEESLKNYCSLLMISPKKARLISLVQPKWQI
                     ILLF"
     gene            complement(560..1882)
                     /locus_tag="PKV31_01535"
     CDS             complement(560..1882)
                     /locus_tag="PKV31_01535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB91561.1"
                     /translation="MLFNEMKIPKPILRALDDMGIAEASAIQELVFSAVKSGRDIGGI
                     AQTGTGKTLAYLVPVLSLHQYSTAKHPSILILVPTRELVKQVEDAIVQLTGYMSVRTY
                     GIFGGVNTKPQADRILEGLDILVGTPGRVVDFVSTGILNLKNIKRLIIDEFDEMLNQG
                     FRAQLKVIFEKLPEKRQNLLFSATLNPEVEEMMDEYFERVENIEATPIGTPLETIEQQ
                     KYHVPNFYSKLSLLKTMLPDQNIRKAIVFVSNKKKAELLFDLLQQTGIDDIAYMHANK
                     HQKRRFDTLDAFESGDSRILIATDLLSRGLDIKDVTHVINFDLPELAENYIHRIGRTG
                     RFGKKGTAISFIEQKDLGLLEALEGMMGTELGERALPADYEQSEELLPEEKERIYMPD
                     YLGQSFTKSDGGAFHEKLKKNKKVNIRFDYEASMKKKYGKQYRPKHAR"
     gene            complement(2000..3112)
                     /locus_tag="PKV31_01540"
     CDS             complement(2000..3112)
                     /locus_tag="PKV31_01540"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNB91562.1"
                     /translation="MNIIFIKKYASLLLNYCMELKSGERLLVSTTTLAEPLVAELYRQ
                     CQQMGVIMEAILEWEGKSDDFNDYGHNAQAGYINPLYKEAMESFEAYLVIRAPFPSKS
                     RSKPNSTLNEVRAAANAQFHKLYFERTATRNLRRNLCQYPTQAGADLAGMSLEEYTSF
                     VANACFLNTDNPEQEWLNVRARQQSITDLLNSCKEFRYLSPNMDISFRTEGRRWINSD
                     GQTNMPSGEIYTSPEENSVNGHILFTLPSLYQGHLLNNVRLEVRDGYITSWTCDNDID
                     ILNEVFRIEGTRRFGEAAIGTNYRIDRLTKNILYDEKIGGTVHMAIGQSYLQAGGKNE
                     SSVHWDMISDMKDGGKIYADGQLVYENGKFLEVILE"
     gene            3231..3530
                     /locus_tag="PKV31_01545"
     CDS             3231..3530
                     /locus_tag="PKV31_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91563.1"
                     /translation="MKAILTILIMFFAFHLCSAQDIIQAQRDQEAKIKKIYNQKKITE
                     LEYSKLMKDQNNIKDAIQIAKADGVMTPKEKNAINKKLMHVEKKIIKYQRNSEIY"
     gene            complement(4386..4547)
                     /locus_tag="PKV31_01550"
     CDS             complement(4386..4547)
                     /locus_tag="PKV31_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91564.1"
                     /translation="MRNERNNTEFRRRFNQISIEWEIALEQGRATNLEFHGEVVLVQV
                     HHQRYYSKK"
     gene            complement(4723..7980)
                     /locus_tag="PKV31_01555"
     CDS             complement(4723..7980)
                     /locus_tag="PKV31_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91565.1"
                     /translation="MTKVPRSKVVRKAPNLYHDNSHDEDNAWNGLPMMAPPTFHLHAK
                     GTYSSLANKDTKPLQLKPASEQKSKKTPDEKIPYKNQGLSAIKDLFEHDAKILAHHNL
                     KLSENHIIKEKNKYQNDRYGYRKEHSNIAKNLEQLMKTQAQIEQYRYPGSNVTFSNAR
                     PPYKDQFLMVLGRATFRINEEGFKKAKAIYPRLQNLYSFIDLLIEKLISNNVFLTGLH
                     ARGKVEEYNQMSGLDQLAAIQEILNDIDFNIKGAREKIISGELSVYDLSPVHQQMASS
                     PKAAGQYRDWRTPGMRDAMQTILQEKKTSDFWKGLGLDALTVMLAVGAGFANPVAAIA
                     MISSNIAISGAKAKSAWDAYSTLNTAYLSRTQGSANSLVSRDQVANAKFDVITETIGL
                     LMDASGGGKDILRMADEISKFGITKGVNQSKKIITQKIGNISTRSGSKLIRPLQRITD
                     IASDKDWIKYLKIKGGYKKVLEEAKGLSGDESAKWLEKMHQFRQRIYKNLREHIKNQG
                     GDIHMTGTPAATSDFDLSIVGPKPEKLRVEAYNHLKNKFGINSKEILNDMLDMNIFGE
                     SRLAHAYDMLPPTIREEAASEILKFSDELILRRGLKNAQDNGDADTVRIILKYMKGHG
                     FTNHAPLPTISSTKINATRLEVGELYHKMENTKNLKQKKDLLIKIGRLQAMIDANSPD
                     YISPGSIRKFVTERPGNVQNPFAPWHTNLAPDDFLKHNKEGLKPRQQAELHAELTGRN
                     NYNKSTTDIIAYEKKINQEIASLNKELEQTLESISDVEKKIANQEISDNIQSLDHQIK
                     LHEKAIVKFKEKKLRAIQKLEEMTAAKANNNQINSLKRYVKNLETKLELAEESISRLK
                     KNLSIMYKHIENSEKVKKLNEQIGQSKTKLKVAGNVKESQNIRNRVRTKVIEEDAGYP
                     KANQNTKKAELESSLVDNLGEAAKYRGTVEQKDLRNLSKYTARMHDILEELAEEIQKK
                     NEFKQLASELNSIKGELNENVAPKRLTELTTRIGDVYKKTLSEMEDFLKSNETKRSKL
                     NKANPYDNRLLQSLLMEFNNIYYKKTALEATQKFILGISQKYAENKVKDVIKDNI"
     gene            complement(8302..8766)
                     /locus_tag="PKV31_01560"
     CDS             complement(8302..8766)
                     /locus_tag="PKV31_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91566.1"
                     /translation="MLGRLKKMLGIDGLRVDLITSETYDIEKGYVDGELSLLAKYDVE
                     IEYIEIRIIEIYTRGRFGNKKTDEYQIGTIRTGFGVNLGANRILPVPFKVPVDYLFSS
                     VDKFGMLNIFTRGLAKGAKLLRGVRSKYRMETEVKIDGSNIKVFNSKPLMIV"
     gene            9289..10698
                     /locus_tag="PKV31_01565"
     CDS             9289..10698
                     /locus_tag="PKV31_01565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB91567.1"
                     /translation="MKIHFCGADKEVTGSSHVVELDNGFTFMLDCGLYQGHDDDMKKF
                     NETWLRPPSSIDCVVLSHAHIDHTGRLPRLVKDGFTGNIYCTHATRSLSSILLVDSAM
                     IQEREAEYSNKKWLQKGIKYHSRITDPLYVETDVPPVLKLMISYGYDKWFKIHEDVEV
                     KYTDAGHILGSAAVNIRIHEGGKVRTLAFTGDIGRPERPILRDPVPMDPAEIVITEST
                     YGDRLHAKAPQDKEKLERIIRHTCIEKRGKLIIPSFSVGRTQEIIYMMDHMFNEGRMP
                     KVKVYVDSPLAVNATSIYIAHTECYDDEMAKYLLVDDNPFGFNGLDYIKNVEDSKAIN
                     DSNEPCVIISASGMANAGRVQHHIINNCDKPSTTILIVGYCALGTPGRLIRDGADTIT
                     LHGQTKPILADVEIMDSFSAHGDRNEMTAFLHNQLESAQTVFLVHGDTDAMTFFGAHL
                     STQGFKNIIMPNLGETFKV"
     gene            10934..11908
                     /locus_tag="PKV31_01570"
     CDS             10934..11908
                     /locus_tag="PKV31_01570"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HNB91568.1"
                     /translation="MLELFRRDQPIGYIFLFLYTILLRAPTFISETNWSPLASSAFSN
                     LVYNMTGYTSVAAQVTGTVLIFIHAVLINMMVAENRITRENSLFPGLFYVLFSTLLPD
                     FNYLSPALMANTFIILTLYNIFNSYKKSGNCSEVFNAGIFIGIAMLFDFSYAIFLICG
                     FVGLLIVRSFRFVERIQFLLGFVIPIFLGGTYYFWMGHLDDFLQTFTTNISWLDWAGN
                     INSQFFLKVGILIFLMLFVIFNYSNYGKKNNVHAMRKVDVMFWFLILPVTTFLFQTGI
                     RLDHALIVAPPMGILLGFSVLNIQNKMIAEVVHLVLISISLLFQYILL"
     gene            complement(12007..13887)
                     /gene="htpG"
                     /locus_tag="PKV31_01575"
     CDS             complement(12007..13887)
                     /gene="htpG"
                     /locus_tag="PKV31_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNB91569.1"
                     /translation="MEQVEKGQITVNTENIFPIIKQFLYSEQEIFLRELISNAVDATS
                     KMKTLSSKGVAPGELGDLTIEVIPDKEAKTLIIRDKGIGLTAEEAKKYLNQVAFSSAQ
                     EFLDKYKEDANIIGHFGLGFYSAFMVADKVEVDTLSYQEEAQPVVWSCSGDTSFEIYH
                     SQRTSRGTDIILHLSDDAIQYLEEDRLAELLDKYCKFLPIPIQLGMKKKTISEGEGDD
                     KQEREIEVPNIINNTNPLWKKSPSDLKDEDYLAFYNELYPFSRPPLFWIHLNIDYPFN
                     LTGVLYFPKIESNFDIQKNKIHLYSNQVFVTDDVKEIVPEFLMLLHGVIDSPDIPLNV
                     SRSYLQADSNVRKITGYITKKVADKLNELFKQDRPAYEAKWPDIGTFIKYGFLSDEKF
                     EEKVGPSILLKNTEGKYFTLEEYKEKIKANQTDKNGKIVILYSNILNDHHSYIQAALA
                     KGYDVVEMDQIIDNHFIQHLEYKKGEFTFKRVDSDVADHLIEKDETIPEVLTESEIKH
                     VDELFKTVLKESMNKVEVKPLSSGEQPVVIVKPEFMRRMTEMQMLQGNAMGKMPEMYT
                     VIINSNHPLINSKLIKMEDSAEKEKFAHYLYDLARLSQQMLTGEELTRFIKENLEKLS
                     NS"
     gene            complement(14060..14917)
                     /locus_tag="PKV31_01580"
     CDS             complement(14060..14917)
                     /locus_tag="PKV31_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91570.1"
                     /translation="MSTPNSLTLLIRESKTDVLAKIKLYLRIAVGVCFIGHGMWGIVY
                     NEAADFLLGKDGWMPLVQLFGFNEHISWALIPLIGLKDILCGIMILYSDNRWLIYWLI
                     FWALFTALLRPVAGFGMVGYIERAGNYGPAIALLYFLNNIWKKELSAEKLKHHISKLE
                     LILRICLSLLIMGHAGLVITDPILTAYGDYKALIANLGVLNYPTDAAWLMALAVFEMT
                     LSLLVLFWPRITGLMLFVLIFKVVSEGMYAFAYGPYSIFETIERFGDYLIPLILMVIY
                     KTWSQEDLA"
     gene            complement(14914..15462)
                     /locus_tag="PKV31_01585"
     CDS             complement(14914..15462)
                     /locus_tag="PKV31_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91571.1"
                     /translation="MERMIVLLIVLTTFYACKNETSEPAPASETTSDFRDGIQSNGVE
                     VNPYLNKEQDIKNNEGKVTRKVRFLPDGRKSVTDYYKGGDTVYLVRYFKNDLQDGKTI
                     GYHPNGKIREIQFFNMGKQFGQDSIFYETGELWYIYSFENDKKNGWMYRYSKDGKQEF
                     AANYKDDKIIEVIDNLKPNKKK"
     gene            complement(15631..>16461)
                     /locus_tag="PKV31_01590"
     CDS             complement(15631..>16461)
                     /locus_tag="PKV31_01590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNB91572.1"
                     /translation="YLHNTEGWSWQDNFKKDGDWKPTPPDFRLPVGGVWGKARTFSLK
                     SEAERLCPPPLPFSEATNSEFYAEALEVYSQNTPTISFEKEWISMFWHDDFLNVTFSP
                     ACRWISVAMQVMEKENTNLRDAVVLLAKMGIAMNDASIACWYSKFYYNVERPVTYIQR
                     VIDPNWEPHLYNAVTGEKGLTPPFPTYPSGHGSMGAAGAEILGSMFGYGHSMTDYSHE
                     TRTDFSGMPRAYSSFLSMTQENAWSRIPLGVHFRMDAEAATNLGTAIGRKVNALPWKK
                     "
BASE COUNT         4421 a         3261 c         3257 g         5522 t
ORIGIN      
        1 ggatttctgg ttggtgctgg tgctgttggc gctggtttca tttgagctct acttttgctc
       61 ttgcttcgac ttttcgacat cgagctctag ttttaatcga tttaaactcg ctcagatgaa
      121 ccttttgatg aaattttaac ctgagcattt cctgtacttc cgctattcaa aatagcagaa
      181 ttatttgcca cttgggctga actagcgaga ttagtcttgc ttttttcggg ctaatcatca
      241 gaagactaca atagtttttt aaactttctt cttccatgtc ggactgaaaa tagctgcttc
      301 taaggccaag gcaaaataga tcgacaagat aaaagcctac aatgagattt ccgtttttga
      361 gctttcggga aacgatgaca ttggctagtt tgttaacttc ccaggtttta ttgatataac
      421 aattaccgat tgggagactt cttgtatttt tccggatgaa attggtagat gtatattgtt
      481 gtttcatttg cttaacattt gaaagacaag gtgggaagtt tttcttgcat tctcataaaa
      541 aacccaataa ttttttttct tatctggcat gtttaggacg atactgcttt ccgtattttt
      601 tcttcataga agcttcatag tcgaatctaa tatttacttt tttatttttt ttcagttttt
      661 catgaaatgc ccctccatct gattttgtaa atgactgccc taagtaatcc ggcatataga
      721 tgcgctcttt ttcttcaggg agtaattctt cagattgttc atagtccgca ggtaatgccc
      781 tttcgcccag ttcagtaccc atcatgcctt ccaatgcttc tagcaatcca agatcctttt
      841 gttcgatgaa ggaaatggcc gttccctttt ttccaaaacg cccggtgcgc ccgatacggt
      901 ggatataatt ttctgccagt tccggaagat cgaagttgat gacatgtgtt acatctttta
      961 tatcaagccc acgggagagc aagtcggtag caatcagaat tcttgaatca ccggattcaa
     1021 aagcatccag cgtatcaaat ctcctcttct gatgcttgtt ggcgtgcata taggcgatgt
     1081 catcaatgcc tgtctgttgc aataagtcga aaagtaattc tgcttttttc ttattggaaa
     1141 cgaatacgat tgccttgcgg atattttgat ccggcaacat ggttttcaac aggctcaatt
     1201 tgctgtaaaa attggggaca tgatattttt gttgttctat ggtttccaat ggtgttccta
     1261 taggagtagc ttcgatattt tcgactcttt caaagtattc atccatcatc tcctccactt
     1321 caggattgag ggtggctgaa aacaggaggt tttgtctttt ttcaggcagc ttttcgaaga
     1381 tgaccttcaa ttgagcccgg aagccttgat tgagcatttc gtcaaattcg tcgatgataa
     1441 gtcttttgat attctttaaa ttcagaatgc cggttgaaac aaaatcgacc accctgcccg
     1501 gggtgcctac cagtatatcg aggccttcca ggattctgtc tgcctgaggc ttggtattta
     1561 caccgccaaa tattccgtat gttctcaccg acatatatcc ggtgagttgg acgattgcgt
     1621 cttccacctg tttgaccaat tcacgagttg gaacaaggat taggatggac ggatgttttg
     1681 ctgtactata ttgatgcagg ctgagtacag gaaccaaata ggcaagggtt ttgcctgttc
     1741 cggtttgcgc gatgccgcct atgtctcttc cggatttgac ggctgaaaat accaattcct
     1801 gaatggccga tgcttctgca attcccatat cgtccaatgc ccttaggata ggctttggta
     1861 ttttcatttc gttgaatagc attcctgtaa ttttggggca aaggtacaaa aaagccgatt
     1921 aacttataat aggctattat tcagacggat ataagttggg cgtgagtgac ttgaggcttc
     1981 aggcttatta ttacttcctt cattctaata tgacttccag aaatttcccg ttttcgtaaa
     2041 ccaattggcc atcggcatat atctttcctc catccttcat gtcggatatc atatcccagt
     2101 gtactgaact ttcattctta ccaccagcct ggagataaga ttgaccgatc gccatgtgta
     2161 ccgtgccgcc gattttttcg tcataaagaa tgttttttgt tagcctgtca atgcggtaat
     2221 tggtgcctat ggctgcttct ccgaaacggc gtgtcccttc aatccgaaag acctcgttca
     2281 ggatatcgat atcattgtcg caggtccatg atgtgatata gccgtccctg acctccaatc
     2341 ggacattatt gagcagatgt ccctggtata gagaaggcaa ggtaaacagt atatgcccgt
     2401 tgacgctgtt ttcttccggg ctggtataga tttcgccgga cggcatattg gtctgtccat
     2461 cactgttaat ccaccggcgt ccttctgtcc tgaatgaaat atccatattt gggctgagat
     2521 atctaaattc cttgcaactg ttgagtaaat ctgtaattga ttgctgtctg gcacggacgt
     2581 tgagccactc ctgttccgga ttgtcggtgt tcaggaaaca ggcattggcc acaaaagaag
     2641 tgtattcttc aagggacata ccggccagat cggctccggc ctgagtgggg tactgacaaa
     2701 gattgcggcg caggtttcgg gtggcagtac gttcaaaata taatttgtgg aattgagcat
     2761 ttgcggctgc tctaacttca tttaatgttg aattgggttt ggatcggctt tttgatggga
     2821 aaggtgccct gataaccaaa tatgcctcaa aagattccat tgcttccttg tatagaggat
     2881 tgatatagcc ggcttgtgca ttatgtccat aatcattgaa atcatccgac ttgccttccc
     2941 attctaagat ggcttccatg atgacaccca tctgttgaca ttgacgataa agttcggcta
     3001 caagcggttc tgcaagtgtt gtcgtactga ccagtaagcg ctcccctgat ttcagttcca
     3061 tgcaataatt gagcaaaagc gaagcatact ttttgataaa tatgatattc attgtatttc
     3121 taatttgtta aaactggata ttggcatcaa atttagccct ttaaatttcc caaattcatt
     3181 ctaattcaat aaatttacgt ttgtatttta tcttttgaag caattttgta atgaaagcaa
     3241 tacttacaat cctaatcatg ttctttgcct ttcatctgtg ttcagcccag gatataatcc
     3301 aggcgcagcg ggaccaggaa gctaagatta aaaaaattta taatcagaag aaaatcaccg
     3361 aactggaata ttccaaactg atgaaggacc aaaacaacat caaggacgcc attcaaatcg
     3421 ccaaggcaga tggggtaatg actcccaagg aaaagaatgc aatcaataaa aaattgatgc
     3481 atgtggagaa aaagatcatc aagtatcaaa gaaatagcga aatctattga tgtagatccg
     3541 atggcatgac ggatttaagc ggactttatc tttggagaca ggcggaatag gagttaaccg
     3601 gaattaggta gatgtttaat aaccttaggc gttgttgttg acttatttcc cgggcaagga
     3661 acttataaaa cctatccgat agaatcgaaa tctgattcat ttacctttgg aattcttttt
     3721 aataggttca ttcacttatt aatcatctac ctacttgggt ttttacatgt catcatcggt
     3781 tcaccggatt tttcctgcaa ttttaatggc cataacggat ttttgagcat gagcgttaac
     3841 gtcgatactg catagttatt aacattattt tgtgggtaac taacattttt ttatcgatat
     3901 aatatcttgg tatttaacga tatagttaag ttattaacat gtgttagtaa gtttcgaatg
     3961 agcaatttgt gtaataagta tctttgtaat gtcaattggg actataacct aaatggagaa
     4021 taatagtttc ccggtgacta aaaacaaatt ttacgttcaa gcgtaaaact catagaccga
     4081 tttaagggag gcagtccgag aggctgcctt tacttttttc atgatgagta tttaatctgt
     4141 gtcgctcttt acaggcctgt gatccttcta tcactgatac gaaaaggtgg tgaaagaaaa
     4201 attccgaata tgtcttaggt atgaaatttg gggaattatt atagtaaaac caaaaaatga
     4261 ttgactttat ttggtgcaat gctataaaat gatttttttt gtggaataaa ataaacagct
     4321 ggatcatgtc tgacaatatt gaaaatgtag gaaaaaaagg atactgtttc ttatacttct
     4381 ttgttttatt ttttgctata gtagcgttgg tggtgtacct gtacaagtac aacttcacca
     4441 tgaaattcta agttggttgc acggccttgc tccaaggcaa tttcccattc gatgcttatc
     4501 tggttgaagc gacgtcggaa ctctgtatta ttccgttcat tacgcacagt tttaatgaat
     4561 attccgctta acgccgaaat gcaatagcgt gctgtcaaac cctcaataaa aatcaggtta
     4621 acgcttcaga aataggggcg tgtaatctca tgtgattgtt tggcgtcttt gattatcaaa
     4681 ttgttgtaac aaaaagcact tagaaaaaat taggagtcat gtctaaatgt tgtcttttat
     4741 tacatctttg actttattct cagcatattt ttgtgatatt ccaagaataa atttttgggt
     4801 ggcttccaaa gcagtcttct tataatatat attgttgaat tccattaaca aactttggag
     4861 gagtcgatta tcatacggat tcgccttgtt tagtttgctt ctttttgttt cattgctttt
     4921 caggaagtct tccatttcgg aaagagtctt cttataaaca tcccctatcc ttgttgtcaa
     4981 ttcagtaagt cgtttggggg ccacattttc attcaattcg ccctttattg aattcaattc
     5041 ggaagcaagt tgcttgaatt cgttcttctt ctggatctct tctgctaatt cttctaatat
     5101 atcgtgcatt cttgctgtat attttgatag atttctcagg tccttttgtt caacggtacc
     5161 tctgtattta gccgcttcgc ccagattgtc caccaaggaa gactcgagtt ctgccttttt
     5221 agtattctga ttggcttttg gatatccggc atcttcctct atcactttgg ttctgacccg
     5281 gttacggata ttttgagatt ctttcacatt tccggctact tttaatttag tcttactttg
     5341 gccaatttgc tcattcaatt ttttaacctt ttcagaattc tcaatatgtt tgtacataat
     5401 ggaaagattt ttcttcaaac gactaattga ttcttcggct aattccaatt tggtctctaa
     5461 attttttaca tatctcttta aactattgat ttgattatta ttcgccttgg ctgctgtcat
     5521 ctcttctaat ttttgaattg ctctcaattt cttttctttg aatttgacaa tagccttttc
     5581 atgaagtttt atttgatggt caagtgattg aatattatca gagatctcct gattggcaat
     5641 cttcttttct acatctgata tactttcaag ggtttgctcc aactccttat ttaaggaggc
     5701 tatctcctgg ttgattttct tttcataggc tatgatatct gtagtcgact tattataatt
     5761 atttcgacca gtgagttctg cgtgaagctc cgcttgttgt cttggtttta ggccttcttt
     5821 gttgtgtttt aaaaaatcat caggtgccag attggtatgc cacggtgcaa atgggttttg
     5881 gacgtttcca ggtcgctccg taacgaattt ccggatagaa ccggggctga tatagtcagg
     5941 actattggca tcgatcatag cttgtagcct tcctatcttt attaaaaggt cttttttctg
     6001 ttttaggttt ttcgtgttct ccatcttgtg atataattcg cccacttcga gtcttgtggc
     6061 gttgattttt gtgctgctta ttgtgggaag tggagcatgg ttggtaaacc catggccttt
     6121 catatatttc aggattatcc ggacagtatc cgcatctcca ttgtcttgtg cgttttttag
     6181 accacgccgt aagattaatt catctgaaaa ttttagtatc tctgaagcag cttcttctct
     6241 aatagtcggc ggaagcatat cataagcatg ggcaagacgt gattctccaa atatattcat
     6301 atcgagcata tcattcaaaa tttcttttga attgattcca aacttgttct ttaaatgatt
     6361 ataggcttcc actcttagct tctccggttt tggtcctaca atagacaaat caaagtcact
     6421 agttgctgcc ggagtgccgg tcatatgtat gtctccgccc tggttcttta tatgctccct
     6481 aagattctta tagattcttt gcctaaactg atgcattttc tctaaccatt tggcggattc
     6541 atcaccactc agcccttttg cctcttcgag cactttttta taacctcctt taattttaag
     6601 atatttgatc caatccttat ctgaggcaat atccgttatt cgttgaaggg gccttattaa
     6661 tttgcttcct gatcttgtcg atatgttccc gattttttgg gttataattt tttttgattg
     6721 gtttacccct ttagtaatgc caaacttgct tatttcatct gccatacgta agatatcttt
     6781 tccaccccct gatgcatcca tcagtaagcc aatagtctca gtaatgacat caaattttgc
     6841 atttgccacc tggtccctgg ataccaaact atttgcactt ccctgagttc gtgaaagata
     6901 agcagtgttt aaagtcgaat atgcgtccca ggctgacttt gcctttgctc cggaaatagc
     6961 tatattgctg cttatcattg ctatcgctgc caccggattg gcaaatcccg caccaactgc
     7021 aagcattact gtcaaggcat ctaatcccag ccctttccaa aagtcactgg tctttttttc
     7081 ttgtaggatt gtttgcattg cgtctctcat gcccggtgtc ctccagtccc gatattgacc
     7141 tgctgctttg ggagagctag ccatttgttg atggaccgga ctcaggtcgt aaacactcag
     7201 ctccccggat atgatttttt cccgggcccc cttgatatta aagtcgatgt cattcaaaat
     7261 ctcctgaatt gcagccagct ggtctaaacc actcatttga ttatactcct ctacctttcc
     7321 ccttgcatgc aatcccgtca agaatacatt atttgaaatc aatttttcaa taagcagatc
     7381 aataaaggaa tataagtttt gaagtctggg ataaattgcc tttgccttct tgaatccttc
     7441 ttcattaatt ctaaaagtcg ctcttcccaa taccattaag aattggtcct tgtaaggcgg
     7501 acgcgcgttt gaaaaagtca catttgaacc aggatagcga tattgctcaa tctgtgcttg
     7561 ggttttcatc aattgctcca ggtttttggc tatattcgaa tgctcttttc ggtagccata
     7621 gcggtcgttt tggtacttgt tcttttcttt tatgatatga ttttcactga gtttgagatt
     7681 atgatgagct aatatcttag catcatgctc gaaaaggtct tttattgctg aaagtccttg
     7741 attcttataa ggtatttttt cgtctggagt ttttttactt ttttgttctg aagcaggttt
     7801 taactgtaat ggctttgtat ccttgtttgc caaagatgaa tatgttcctt ttgcatgtaa
     7861 gtgaaaagta ggcggagcca tcataggcaa accgttccat gcattgtctt catcatgact
     7921 attgtcatgg tataggttag gcgctttcct gaccactttt gaacgtggta cttttgtcat
     7981 tattgaaaaa ggttagacta atacaaataa agataacata ctctccggct taatacatca
     8041 ttcctgtttt caggattgaa tttccttata ctttgtttat ttgtaaagat aaataataaa
     8101 acaatatacg ggaagcttcg tatagtgtta ttttaacaaa taaggggagt aaatctttat
     8161 tcttgtaaat ggcagaaatt tattattaac agccttcaaa atttaagcaa gatttacctt
     8221 ttaaatgtat tcaatggagt tgatcacagg atatataccg tgagggataa atctttttca
     8281 tcaagcaaaa taaatatttc cttaaacgat catcagaggc ttgctgttga agaccttgat
     8341 atttgatccg tctatcttta cttccgtttc catccgatac ttggatcgga ctccgcgtaa
     8401 tagttttgcg ccttttgcca atcctctggt aaaaatgtta agcattccga atttgtcaac
     8461 ggaggagaat agataatcta caggcacctt aaatggtaca ggcagaatcc tgttggcccc
     8521 aaggttgaca ccgaagcctg tccggatagt cccaatttga tattcgtcag tctttttatt
     8581 accaaaacgg ccgcgagtgt atatttcaat gatccgtatc tctatgtatt caatctcgac
     8641 atcatatttt gccagtagac tcaactctcc atcgacatat cccttttcga tatcataagt
     8701 ctctgaagta atcaaatcaa cccgcagtcc atcaatacca agcatttttt ttagtcgtcc
     8761 caacatcact acacactttt tatctacaac aaaaatgcac tttatttcat tactaaaaat
     8821 tgattttttt cgatggatcg atgatgcttt tctttctaat agtggaattt ttggacaaat
     8881 gtgcactgaa ctttatcaga aatatgacga cagccggtgg ggtagctttg ttcaggcttt
     8941 ttgatcgatg cacatgtcat ccttaatagt gtaaaagata attaccaaac tagtattggc
     9001 ccttcttaat tgtgattcct cttcttcatg ttttgtcact tctcctgcgc atgccttttc
     9061 tttgtcgagt ggttctgctc caatttaact tttgactatg ttgctaatgg tatataaaac
     9121 ttttggcaaa cattataagt tattaaaaaa taaaatatat tattataagg aatacatgtg
     9181 gttatgaatg attttatggc cattgttctc gtgctttgat tatgggtgac aattattatt
     9241 tccatttttt tgatgatttt accttaactt gccttaaaat agggaataat gaagatacat
     9301 ttttgtggtg ccgataagga agtgaccggt agtagccatg ttgtggaact ggacaatggt
     9361 ttcactttta tgttggattg tggcttgtat cagggacatg atgacgacat gaagaaattc
     9421 aatgaaacgt ggctaaggcc tccttcctcc attgattgtg tagttctgtc ccatgcgcat
     9481 attgaccata caggtcgact gcctagattg gtgaaagatg ggtttaccgg taatatatat
     9541 tgtacccatg cgacccgctc actttcaagt attctattgg ttgattctgc aatgatccag
     9601 gaacgggaag cggaatattc taataagaaa tggttgcaaa aaggaattaa atatcatagt
     9661 cggatcaccg atcctctata tgtagaaacc gatgtacctc ctgtgttgaa actaatgatt
     9721 tcctacggat atgacaaatg gtttaaaatc catgaagatg tagaggtgaa atataccgat
     9781 gccggtcata ttcttggatc ggctgcagtt aacattcgta ttcatgaagg gggtaaggtg
     9841 aggacacttg cattcaccgg ggatattgga aggccggaac ggcccatact aagggatccg
     9901 gtaccaatgg atccggctga aatagtcatc acggagtcaa cctacggaga tcgattacat
     9961 gccaaagcac cccaggataa agagaaattg gaaagaatca tacggcatac ctgcatagaa
    10021 aagcgtggta aattgattat tccttcattc tctgtgggtc gcactcagga gatcatctac
    10081 atgatggatc atatgtttaa tgagggcaga atgccaaaag tcaaggtata tgttgatagt
    10141 ccactggcag tcaatgccac atccatttat atagcgcata cggaatgtta tgatgatgag
    10201 atggccaaat accttctggt ggatgacaac ccatttgggt tcaacggatt ggactatatc
    10261 aagaatgtgg aggattccaa ggcgattaat gacagtaatg agccatgtgt aatcataagc
    10321 gcgtccggca tggccaatgc cggaagagta caacatcata tcatcaacaa ttgtgacaag
    10381 ccgagtacaa ctattttgat tgtcggatat tgtgcattgg gaactcccgg ccgattgatc
    10441 cgggatggag cagataccat caccttacac ggtcagacaa agcctattct ggccgatgtt
    10501 gagataatgg actctttttc agcacatggc gacaggaatg agatgacagc ctttttgcac
    10561 aatcagctgg aaagcgccca aacagttttt ttggtccatg gcgatacgga tgcaatgacc
    10621 ttttttggtg cacatctgtc aacacaaggt ttcaaaaaca ttatcatgcc taatctcgga
    10681 gagaccttca aagtttgatg gtgtgaaatt tggggacttt taaccaaatt ccaacttata
    10741 caaggcatta atgcactctg gtattgagat aatttggaaa gatcagtgtc aatttggcgt
    10801 gaaatgatgc cgttcatgtt ttcattgtct ttagagattg aggtcaatga actgatggtg
    10861 atgcaccatc gacattcagt atatgctatt cattggaaaa aaattacctt tgtcccgata
    10921 aaaccggctt gtcgtgttag agttattcag gagagatcag ccaattggct atattttcct
    10981 tttcttatat actattttgc tgagagctcc gactttcatc agtgagacca attggtctcc
    11041 tcttgcaagt agtgcattca gcaatctggt ctataatatg accgggtata catcggtggc
    11101 tgctcaggtt acgggaacag ttctgatctt cattcatgca gttctcatca atatgatggt
    11161 tgcagaaaac aggataacac gtgagaatag tcttttcccc gggctattct acgttttatt
    11221 ctcaacctta cttccggatt tcaactatct gtcaccggct cttatggcca atacctttat
    11281 cattttaact ctttataaca tattcaactc ctataaaaaa tccggaaatt gcagcgaggt
    11341 gtttaatgcc gggattttca tcggcattgc gatgctattt gattttagct atgccatttt
    11401 tttaatttgc ggatttgttg ggttattaat tgtcaggtcg ttcagatttg ttgagcggat
    11461 tcagttttta ttgggatttg ttattcctat tttccttggt ggaacctatt atttttggat
    11521 gggacatttg gatgattttt tacagacctt taccaccaat ataagctggc tggactgggc
    11581 aggcaatatt aatagtcagt tttttcttaa agtcggtatt ttaatcttcc tgatgttatt
    11641 cgttattttt aattacagca actatggcaa gaagaataat gtccatgcga tgagaaaagt
    11701 agacgtgatg ttttggttct tgatccttcc tgtgactaca tttttgtttc agaccggtat
    11761 ccgattagac catgctttga tcgttgcacc tccaatgggg atcttactgg gtttctccgt
    11821 tcttaatatt cagaataaga tgatagcaga agtagttcat ttggtcttga tcagtatctc
    11881 attgttattt caatatattc ttctatagtt aatagctttt ccgggaagta ttttccgggt
    11941 agtggataaa aaaatagccg gggacaaatc ccggctaaat ctttttacct gatagatcta
    12001 tcagaatcaa ctgttgctca gtttttcaag gttttcctta ataaacctgg tgagttcttc
    12061 gcctgtgagc atttgctgac tgagtcttgc caaatcatag agataatgtg caaacttttc
    12121 cttctcagcg gaatcttcca tcttgattaa tttgctgttg atgagaggat ggttgctatt
    12181 gataatgacc gtgtacattt ccggcatctt acccatcgca tttccttgga gcatctgcat
    12241 ttcagtcatt cggcgcatga attcaggttt gacgatgaca acaggctgtt cccctgaaga
    12301 caagggtttc acttccactt tattcattga ttcctttagc actgttttga atagttcatc
    12361 tacatgctta atctccgatt cagtcaaaac ttccggaatg gtttcatcct tttcgatgag
    12421 atggtcagca acatctgagt caactcgctt gaaggtaaat tctccttttt tatactccag
    12481 atgctggatg aaatgattgt cgataatttg atccatctca acgacatcat atccttttgc
    12541 caaggcagcc tggatatacg agtgatggtc attgagtatg ttggagtaaa gaatgacaat
    12601 ctttccgttt ttatcggttt ggtttgcttt aatcttttcc ttgtattcct caagggtaaa
    12661 atatttgcct tctgtattct ttaacaatat ggaagggcca accttttctt caaatttctc
    12721 atcggaaaga aaaccatatt tgataaatgt tcctatatcc ggccacttgg cttcgtatgc
    12781 gggtctgtcc tgcttgaata gttcattgag cttatcagct acctttttag tgatgtaacc
    12841 tgtgatcttt ctaacattgc tgtcggcttg cagataagat cgcgatacat tgaggggaat
    12901 atcaggtgag tcaattacac cgtgcagtaa catcaggaat tccggtacaa tttcctttac
    12961 atcatccgtt acaaatacct gattggaata aagatgaatc ttgtttttct gaatgtcgaa
    13021 attgctctcg atcttcggaa aatataacac acctgtcagg ttgaaaggat agtcgatatt
    13081 gaggtgaatc cagaacaggg gtggtctgct gaatggatat aattcattat aaaatgccag
    13141 ataatcttca tccttcaggt cagaaggtga ttttttccat aagggattgg tattgttgat
    13201 gatattagga acctcgatct ccctttcttg cttatcgtca ccttcgccct ctgatattgt
    13261 ttttttcttc atacccagct gtatgggtat cggcagaaat ttgcaatatt tatccagcaa
    13321 ttccgccagt ctatcctctt caagatattg tatggcgtca tcactcagat gtaagataat
    13381 atcggtacct cgactggttc tttgcgaatg gtatatttca aaacttgtat cgccactgca
    13441 tgaccatact acaggttgtg cttcttcctg gtaggaaagt gtatccacct ccaccttatc
    13501 cgctaccatg aatgcggaat aaaagcccaa tccaaagtgg ccaatgatgt tggcgtcttc
    13561 cttgtattta tccaggaatt cctgtgcgct cgaaaaggct acctgattga ggtatttttt
    13621 tgcttcttct gctgtgaggc cgataccttt atcccggatg atgagcgtct tagcttcttt
    13681 gtcgggtatg acttctatag tcagatcgcc caattcacca ggagccacac cttttgatga
    13741 tagcgtcttc atcttggaag ttgcatcaac agcattcgat atcagttccc tgagaaaaat
    13801 ttcctgttca gaatatagaa attgcttgat gatgggaaag atattctcag tattgaccgt
    13861 aatttgtcct ttttccactt gttccatatt taatttattt tataagaaaa tagcatagca
    13921 ataagcattc cttgacttga gattatgtca atttggcaag ttggaatgaa gaaatgacag
    13981 atgaaaaaaa ataaaaaggc aatttatcaa caataaattg ccttttcagg tgatatttag
    14041 tacctgatga tctaatggat taggcgaggt cttcctgact ccaggtttta tagatgacca
    14101 ttagaatcaa aggaattaaa taatcaccaa agcgctctat ggtttcaaag atggagtatg
    14161 gtccataagc aaaggcatac attccctcac tgacgacctt aaagatcaag acaaacaaca
    14221 tgagaccggt aattcttggc cagaacagga cgagaagact cagagtcatc tcaaagacag
    14281 ccaatgccat caaccatgca gcatcagtcg gatagttcag tacaccgaga ttggcgataa
    14341 gggctttata gtctccatat gcagtcagga ttgggtcggt gatgaccaat ccggcatggc
    14401 ccatgattaa caaagaaaga catatcctga ggattaattc cagcttactg atatgatgtt
    14461 tcagtttttc cgcagaaagt tcttttttcc agatattgtt cagaaaatat agcagggcaa
    14521 tggcgggccc ataattaccg gctctctcaa tatagccaac catgccgaag ccggctacag
    14581 gccttagcaa agcggtgaag agggcccaga atatcaacca ataaatcaac cagcggttat
    14641 cactatacaa tatcatgata ccacacaata tatccttcaa gccaatcaat gggatcaatg
    14701 cccaggatat gtgctcattg aatccgaaga gctgtaccag aggcatccaa ccatctttcc
    14761 ccagaagaaa atcggcagcc tcattatata cgatgcccca cataccatga ccgatgaaac
    14821 aaacaccgac agcaatacgc aggtataatt ttatttttgc caggacatcc gttttagact
    14881 cccgtatcaa taacgttaaa gaatttggcg tactcatttt ttcttgtttg gttttaggtt
    14941 gtcgataact tcaatgattt tgtcatcctt gtaattggct gcgaactcct gtttcccatc
    15001 cttggagtaa cgatacatcc aaccattttt cttatcgttt tcaaaggaat aaatatacca
    15061 aagttcgccg gtctcataga atatggagtc ctgaccaaac tgtttaccca tgttgaaaaa
    15121 ttgtatttcc cgaattttac cattcgggtg atatccgatg gtcttaccat cctgtaggtc
    15181 atttttgaag taacggacca ggtatacagt atctccaccc ttgtaataat cagttacgga
    15241 ctttctgcca tcaggtagga atcggacttt tctggttacc ttgccttcat tgtttttgat
    15301 atcctgctct ttattaaggt atggatttac ttcgacgccg ttggattgaa ttccatcacg
    15361 aaagtcggaa gtggtttctg aagccggggc cggttcggaa gtttcattct tgcaagcata
    15421 aaaagtagtc aacacaataa gtaatacaat cattctttcc ataataattt tattgttctt
    15481 tacaagataa caaaatttat ccgatcatcc ggtaaacaat taagaaataa ccttctatcc
    15541 ctaagaagag cctttgagaa aagaggtatt aaacaaattt atttttaagg caaaaaaaag
    15601 taccccggcg gggtactttt ttcgattaag ttattttttc cagggaagag cgttgacctt
    15661 tctaccaata gctgtgccga ggttggtagc agcttctgca tccatcctga agtgtacacc
    15721 cagtggaatc cgcgaccatg cattttcctg tgtcatagac aagaagcttg aataggctct
    15781 tggcatacct gagaaatctg ttcttgtttc gtgggagtag tcagtcattg agtggccgta
    15841 accaaacatg ctgcccagga tttcagcacc ggcagcgccc atggagccgt gccctgaagg
    15901 ataagttggg aaaggaggag taagtccttt ctctccggtg acggcattat agaggtgagg
    15961 ttcccaatta ggatcgatta ctctttggat gtaagtcacc ggtctttcga cattataata
    16021 gaatttggaa taccagcaag ctatagatgc gtcattcata gctataccca ttttggctaa
    16081 caatacgacg gcatcacgaa gattggtatt ttctttttcc attacctgca tagctacact
    16141 tatccaacgg catgcaggac taaacgtaac attgagaaag tcatcatgcc agaacataga
    16201 tatccattct ttttcaaagg aaatagtcgg ggtattctgt gaatagacct ctaaagcttc
    16261 agcataaaat tctgagttgg tagcttcaga gaatggaaga ggtggaggac atagtctctc
    16321 tgcttctgat ttcaaggaaa aagttcttgc cttaccccat acgcctccta caggaaggcg
    16381 aaaatcaggc ggtgttggct tccagtcgcc atctttcttg aagttgtctt gccagctcca
    16441 accttcagta ttatgcaggt a
//
LOCUS       DAOQMC010000020        16433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1270_length_16433_cov_4.941018, whole genome shotgun sequence.
ACCESSION   DAOQMC010000020 DAOQMC010000000
VERSION     DAOQMC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16433
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_16433_cov_4.941018"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKV31_01595"
     CDS             complement(<1..210)
                     /locus_tag="PKV31_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91573.1"
                     /translation="MIRFLIAIILTTSMNVVTPAQIETISTKDTTTYSEKQAMAEDRF
                     VQATSLRLIGKSKEAIPILQELIKQD"
     gene            complement(408..1193)
                     /locus_tag="PKV31_01600"
     CDS             complement(408..1193)
                     /locus_tag="PKV31_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91574.1"
                     /translation="MSSKYITLIMIYLATGIIYLTAGLSKFSPGNIGNLIGPVDYRKI
                     SDSTVFLLFMQGIAVLQVVVGALLLSQRYSVFGLIMVLPMSLGILIFTIVAGFGLTPI
                     INLLLLLLLLYAFYLEKEAIRQLFRKDRKGILFKSPSFQQFPERLVPNIALILIGLTV
                     LVSFLDNIVLNILTTTALLLFTINLFQRKDYIFADKILIFLFLMISSITVNGMLINKM
                     IAKGFYVVFLLIPMGILIYLFRLVYWKFSEKNVQDRQNQVKEG"
     gene            1253..1396
                     /locus_tag="PKV31_01605"
     CDS             1253..1396
                     /locus_tag="PKV31_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91575.1"
                     /translation="MMAFHGGAHYNDNIKKNYGKNGKIGYSITFITGSLSGCTSISSP
                     YRQ"
     gene            complement(2052..2453)
                     /locus_tag="PKV31_01610"
     CDS             complement(2052..2453)
                     /locus_tag="PKV31_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB91576.1"
                     /translation="MITIKALIKADVQKVWDYYTLPHHIIRWNFAGPGWHCPKAENDM
                     RPGGTYKARMEAKDGSFGFDFEAIYTDVQPGKEFSYEFGGRKASVSFSKNGEETEIVI
                     CFDPETTHSIELQRSGWQSILNNFKSYTENN"
     gene            complement(2513..2869)
                     /locus_tag="PKV31_01615"
     CDS             complement(2513..2869)
                     /locus_tag="PKV31_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB91577.1"
                     /translation="MSAQKKIVEKYMEGFRRTDHKEILSCLTEDVVWEMPGFYLHRGI
                     EAFDKEIENPNADGHPDIKVIRLIEEGNIVVAEGSVKATLKDGKKLDAVFCDVFHFRG
                     GKISKLTSYLMFYNLP"
     gene            complement(2943..4028)
                     /locus_tag="PKV31_01620"
     CDS             complement(2943..4028)
                     /locus_tag="PKV31_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB91578.1"
                     /translation="MKKKKNSILRQIAKYILRGIGIIILIFLTWSMIPIRQTIRPIQK
                     RAETKYWTMRDGSKIAYSLLKGDSANINPPIIYLHGGPGGYVHSAIIKQMDEVAKKGY
                     DVYLYDQIGSGLSDRLSRPKDYSFERHLNDLKEIINSHIMSDKVILLGHSFGGILAVH
                     FAARFPNSIDRLILSSPGDLQPYRVNADGTMADMGRLYPTPANYQFKAPIEVFEQTEK
                     DFLQPRIVMSMFCALAFNIKWASDKEFDDYTNTMASKFTLGMVADPRNVKMEEGGAGG
                     YSHGFSNYYGDLKDAREKLRNLNIPTLVMQGQYDQGEYSSVYEYVDLLKGNYIFIEDA
                     GHIIWWDKPNEFNRSVIEFLMKKSSVQ"
     gene            complement(4195..4410)
                     /locus_tag="PKV31_01625"
     CDS             complement(4195..4410)
                     /locus_tag="PKV31_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB91579.1"
                     /translation="MAIIVNLDVMMAKRKMSLNELSEKVGLTLSNLSILKTGKAKAIR
                     FSTLEAICKVLECQPGDILEFVEEDRD"
     gene            complement(4414..4950)
                     /locus_tag="PKV31_01630"
     CDS             complement(4414..4950)
                     /locus_tag="PKV31_01630"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HNB91580.1"
                     /translation="MSKTNNFVFWALYSVAWIIFVGLSIEAGGLLVNFIISLYKPELI
                     QSLYQKLDLTEIYKESRLAFFCYYSFILTIAILKACLFYTVIRLMHTMDMSKPFSDFV
                     AKQIALISYYTLVIGFLSYLAHQVTKTMMHHGLVTDKLRPFWEDSDAYILMGGVVYII
                     GVIFRKGVAIQNENDLTV"
     gene            complement(5536..6147)
                     /locus_tag="PKV31_01635"
     CDS             complement(5536..6147)
                     /locus_tag="PKV31_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91581.1"
                     /translation="MNNTIQRMFDKEEIIRQVAQVLKETRTAQDTVRQGPGRDRRFTL
                     LARYMIEKAIRHNALLTDDTGTGIALMFRMNAGRNHFFRDVWEDIKLAFCVIGLTNVP
                     KIIRVRKHIRSRQPKSEPFFYMWFWGVIPGSRGAGKGVAVNMKDMFLRISEDEQLPIY
                     AETRLRTNAMVYQRYKFIHYDTWNMPGGDTMWFVRYLPKSLGR"
     gene            complement(6169..7095)
                     /locus_tag="PKV31_01640"
     CDS             complement(6169..7095)
                     /locus_tag="PKV31_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNB91582.1"
                     /translation="MTYFTHTILIISIFEKSIVNPMKNLFLSLLLIFPALVQAQELKP
                     YQFYNSRGKAVTFGKMINSLKDYDVVLFGEYHNNSVNHWLELKTAEALFEKKGNRLIL
                     GCEMYERDNQEAIDRYLSGDINDEKLGEEARLWNNHKTDYLPILEFARQKKLLMIATN
                     VPRRFAALVAKKGKDSLKLESHEVPWVVKLPYEVSMETPGYDEMLKMMGDHAGSRAMN
                     FVAAQAIKDATMAESILNHLSPGTLFFHYEGDFHSKEHGGIYWYLKKARPELKIAVIS
                     ISESADPALKLPSDFKKTEFNLVMPQDMTKTY"
     gene            7376..8188
                     /locus_tag="PKV31_01645"
     CDS             7376..8188
                     /locus_tag="PKV31_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB91583.1"
                     /translation="MHFYEKTVLITGGCSGIGKIMARKSLERGASRVIIIDVNDAVFQ
                     DLKKEWEAFSGEVITFHADLSKVDQIRDVAARVKEAYGVPDVLINNAGIVTGKYFYEH
                     SHENISLSMAVNADAMMHLTLEFLPGMIERNSGSIGNLCSLAGLIGNTKMAVYVASKW
                     AAVGWSESLRLEMQQLGKDIKVTTIMPYYINTGMFDGVRSGLIPILKPEPSSEKFIRA
                     IERERKLFATPVPYWFIRLSQGILPVSAFDFVMKNIFGVYDTMKKFKGRDGN"
     gene            8729..9295
                     /locus_tag="PKV31_01650"
     CDS             8729..9295
                     /locus_tag="PKV31_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB91584.1"
                     /translation="MIEKTEMHRISAWILKMLGWKIVGWDMNALKKYVLIAMPHTSNL
                     DFPLGLLVRSSIKLKIHFVGKSGLFRWPFGFIFRRLGGYPVDRSRSHNYVEAVAAMFK
                     DKDLFRLTIAPEGTRSKVDRLKTGFYYIAQTARVPIVMVAFDYKTHEIRVHPPIDSSI
                     SYDDLYQKMKEFFRGVEGRHPEKDYEFE"
     gene            9458..10903
                     /locus_tag="PKV31_01655"
     CDS             9458..10903
                     /locus_tag="PKV31_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91585.1"
                     /translation="MFRIIPIALFCLLAYISNGQTASEDKINTANKALAFLNKIADMN
                     KSGSRAFTLKFDTLSIFFAGPEEPESLVSTMPVTYDAWGRIKDARIIEAGFEDYYQYR
                     YNYTGDDNRFSHLDYYLHSGGEEHYAGVQIHLYDDHGRLISSRELDKDNQLIYGDSLS
                     LEFNGLGQIVFYEYLYFQLGRWNSGGKVYDVQYSGQDIVGYNADEAERGHSGKDTIVS
                     YRYEDVRFLNDDSSILFPDFDEFFEIDSLGVDNFFLSSRDAAKAYVKQPVQYLKYRTD
                     GSGALVQSAVYDPDKFISIISDPNGKEIYRIEYSFDGQGRLKGIHKVETGQVPEVSTF
                     EYNELGRMARYIFTSDSVNFRVNNLYVTDDKDRLSEYYQNLEDTNNSITNILRFGYRG
                     ENKTYTGSILEACVFPNPAGHAIYIKTPQSIYQEMQVSMMGNDGRLVFSGRINFTAGV
                     GSIPVAKVPPGTYLLLIKDGKKAYKATVVII"
     gene            11065..12723
                     /locus_tag="PKV31_01660"
     CDS             11065..12723
                     /locus_tag="PKV31_01660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB91586.1"
                     /translation="MKRTKGNLIRSLMAGLNKAVHEYQLETSSKEGVARYNRRTFIQQ
                     STKGAIGVGVALSLPAMLISCKQETRPPQGVEQDPVLDVAILGGGMAGLNCANHLLGS
                     GLTFKVYEGSKRVGGRILTHYNDSMQLGIFPEFGGDFIDSDHEDMIKLAKEFNLDLVD
                     QIAEQEANQWVKDIYFFDNRKISEKEIIREFKKIAPKIAKDRDALGENYDTPEASRMD
                     KMSLSEYLDSLKCKEWMKELLDAAFVAEFGLDCSEQSAINMLDLIITDTSEGFKVFGT
                     SDERYRIKGGNSQITNKLAEKIGEDNILKNYKVTEISEGDNGMYTIVFENGEKVEAKV
                     IVCTIPFTILREIKLSLKGMTPEKRKCIDELGYGINTKLVLGYEGQPWRSKENNAMGY
                     LFTKDMTNGWDASLNRTENNENGAYVAFFGGKFSQSLCDESSRNPMSPPEHVWRTMLP
                     EKRVNGFVDDLDKIFIRSKKKFKGKHVFVNWIEYPFVKASYACYKTGQWTTIAGHEAK
                     PVGNFYFAGEHCSNNFQGFMNGAAESGRETAIQLIEKYAKVPVN"
     gene            complement(12975..13361)
                     /gene="apaG"
                     /locus_tag="PKV31_01665"
     CDS             complement(12975..13361)
                     /gene="apaG"
                     /locus_tag="PKV31_01665"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNB91587.1"
                     /translation="MTSLTTKGVNVDIKVDFHTESDTDGRAGFIFIYHISIRNHNRFP
                     VQLLSRHWRIFDSNGEVRYVDGEGVVGLQPIIEPDQIFDYSSFCNLSTELGTMSGTYT
                     LENLHSGNLFEVDIPTFELATPAIMN"
     gene            complement(13555..14073)
                     /locus_tag="PKV31_01670"
     CDS             complement(13555..14073)
                     /locus_tag="PKV31_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91588.1"
                     /translation="MMNIDTESKSSVFDSVKSYLASIGLAFRSIDDSRPWGGFFTFEE
                     NLAEAFIMHFYSGTEIHFKDFPEGATLSPKLLVVAPGQRLSWQYHFRRAEVWRCIHGD
                     ITVITSDTDEEKYSRILESGDIIRLAQGERHRIIGNDDYGIIAEIWQHTDASNPSNEE
                     DIVRLQDDFGRK"
     gene            14308..15699
                     /locus_tag="PKV31_01675"
     CDS             14308..15699
                     /locus_tag="PKV31_01675"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNB91589.1"
                     /translation="MKKFLTKFGRPLLFLTLSLFAIQCSESDYDIEPQYKDASHFEGD
                     VAIEWNSILNELDRYTEGYKSPIAARAMAFVNLAAYEAMIPGMAGKYISLAKDNFRVS
                     IPAPEKDKAYFWPLVLNTCYKVAIQKFYPTAPSAQQLAILDTYEKLQKKYSEKISPET
                     VLRSLEYGGTIAGLIYDFSTKDELADAAYQRVNDPNYIPPAGEGNWQPSPPDFAPAVL
                     PYWGNVRTLVAVDSDFWISGPPEYSTVPQSRMYKETKYVHDRGNFLRDNPENESNWIA
                     QFWSDDFSPLTFSPASRWNAITTQILQERESNLEEAVMSYLKVSFALSDAAIGCWQNK
                     YKYNVGRPVTYIRANMDADWNTLMKPGLEEGFYTPATPSYPSEHATYAAAATGVLANL
                     FGNECNFTDHCHEGRTEFKGNPRTFYKFSQMAEESGFSRTQIGVNSQFDVDGGLQLGA
                     QVAKAINDFRFQK"
     gene            complement(15719..15994)
                     /gene="yidD"
                     /locus_tag="PKV31_01680"
     CDS             complement(15719..15994)
                     /gene="yidD"
                     /locus_tag="PKV31_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNB91590.1"
                     /translation="MYYLNRLLKYVLILPIRFYQLSISPLLGTNKCRYQPTCSHYAIQ
                     AIEEWGVLKGSWLGFKRIMSCHPWGGHGFDPVPDNPQKKNGDRNDHH"
BASE COUNT         4812 a         3170 c         3547 g         4904 t
ORIGIN      
        1 gtcttgtttg atcagttcct gaagaatcgg aatggcctct tttgacttgc caatcagcct
       61 gagggatgtc gcttgcacaa aacgatcctc cgccatggct tgtttttcac tataggttgt
      121 tgtatctttt gtcgaaatgg tttcaatctg ggcaggcgtc acaacattca tacttgttgt
      181 caaaataatt gcaatcagaa atcgtatcac actgtaattt ttttcagaat attaacgtat
      241 tttaatggga gtagttgtaa cgatggattc aaatttgaat aaataattgc ttgcctgcaa
      301 tccattcgct gtgtttattg atgccggacc ggatttcagc ggaaaatgag gtgtggtcaa
      361 ataccgcctt ttcatagggc ctttgtcata gtcccgatta caacacctca tccttcctta
      421 acttgatttt gacggtcttg gacattcttt tcggaaaatt tccaatatac caacctgaac
      481 aaataaatta aaattcccat tggaatcaac aggaagacaa cataaaaacc ttttgcaatc
      541 attttattga tcaacatacc attgactgta atagaactga tcatcaggaa tagaaagatg
      601 aggatcttat cggcaaagat atagtctttt ctttggaaca ggtttatggt aaaaagtagc
      661 agggcagtgg tcgtaagtat gttgagaaca atgttgtcaa ggaaggaaac aagaacagta
      721 aggccgatca gaatcaaggc aatatttggt accaatcgtt caggaaattg ctgaaatgaa
      781 ggtgatttga aaaggatgcc cttccggtct tttcggaata actgcctgat tgcctccttc
      841 tccaggtaga atgcgtacag gagaagcaac aaaagcaaaa gattgatgat gggtgtaagc
      901 ccgaagccgg caacgatggt aaaaatcaga atgcccaggg acataggtaa taccatgatc
      961 agcccaaata ccgaatatcg ctgggacaat aaaagtgctc cgacaacaac ttgaagcacg
     1021 gcaatccctt gcatgaagag gagaaagacg gtggaatcgg agatcttgcg ataatcgacc
     1081 ggtccgatca gattgcctat attgccgggt gaaaacttac tcaatccggc agtcagataa
     1141 atgattcctg tcgccaggta aatcatgata agcgtaatgt attttgaact catagcgtcg
     1201 atgtcgaagg gttgaaatgc atgtgatttg agtaaattgg aagcctctcc tgatgatggc
     1261 ctttcatggt ggggctcatt acaatgacaa tattaagaaa aattatggta aaaatggcaa
     1321 aattggctat tcaataacct tcatcaccgg ttcattgtcc ggttgtactt ccatctcctc
     1381 gccttacaga caatagatac aaaattggtt ttagattggg gaataaaaag tcgtaatcgc
     1441 tgacaatgga aactgtatcc acattgacct ccaccaatta catatttact taacagggtt
     1501 ttaataacga tttatcgcca agacttcata gtcgtctttc atcttttaat aaaaaacata
     1561 gtgtctggat tcggaaataa gaatattgac tattagagga attgcaaaac atccctgctg
     1621 tacgtttgat aatcaactaa gggtatagca taaagttatc cgagagtaga gattggctgt
     1681 aataaaccgg catttaataa caatgcaggc ttgaggtact tacaactctc aacttgatca
     1741 gcctgccagg tgaagcgcat gccgcgtaag cttttacttt tttcagtatt aagagaaatt
     1801 aagttgctca gacttcgaag tgtattgttt gcgggtgttc cttttttaca tttagatgat
     1861 gaaattgtat tcaaatgcgt gtgtgtctca aacttgcggc gttccaattg ccggtttttg
     1921 atttttttag ccgagcctcg aatatgaaca tagattctat ccgtttttta caatttcaaa
     1981 atgcggtata ttctataggt tcattttatg caagccggcc aaaaaagtga gcactaacta
     2041 tgaaaataca gttaattatt ttccgtgtat gacttaaaat tattcagaat ggattgccac
     2101 ccactccttt gcaattctat ggagtgggtt gtttccggat caaaacaaat tacaatctcc
     2161 gtttcttctc cgtttttgct gaatgagact gaagccttac gcccaccaaa ttcataacta
     2221 aattctttgc caggttgaac atccgtatat attgcttcaa aatcaaaacc gaagcttccg
     2281 tcttttgctt ccattcttgc cttgtatgtt ccgcccggtc tcatatcatt ctcagccttg
     2341 gggcaatgcc aacctgggcc tgcaaaattc catcgaataa tgtgatgtgg aagtgtatag
     2401 taatcccaca ctttttgtac atctgccttt ataagagcct ttatggtaat catgatgttc
     2461 tctatttttt atttcacaat tttaacccat tggtattaac ttaaaatgta agttacggta
     2521 gattgtaaaa cattaggtaa gacgttagct tgcttatttt tcctcctcta aaatgaaaaa
     2581 catcacaaaa cacggcatct aatttctttc cgtctttcaa agttgctttt acacttcctt
     2641 ctgcgacgac tatatttcct tcttcaatca accggattac ctttatatcc gggtgtccgt
     2701 cagcattagg attttcgatt tccttgtcaa aagcctcaat ccctctatga agataaaaac
     2761 ccggcatttc ccaaacgaca tcttctgtca ggcatgacaa tatttcttta tggtcggtcc
     2821 ttcggaatcc ttccatatac ttttcaacta tttttttctg agcgctcata tttacttggc
     2881 taccttaaga ttttaaatta gttaaagtta gcaataggga atgattcgga tcaaattatt
     2941 gtttattgaa cgctactttt tttcatcaga aactcaatga cagaccggtt gaattcattg
     3001 ggcttatccc accatatgat gtgtcccgca tcttcgataa atatatagtt gcctttgagc
     3061 aagtccacat attcatatac ggaggaatat tccccctggt catattgccc ttgcataacg
     3121 agggtcggga tgttcagatt tcgaagtttt tctcttgcat ctttcagatc tccgtaataa
     3181 ttgctgaatc cgtgcgaata tccacccgcc ccgccttctt ccattttcac attccttggg
     3241 tcggctacca ttcccagggt aaatttagat gccatcgtat tagtataatc atcaaactct
     3301 ttatcacttg cccatttgat attaaaagcc aaggcacaaa acatcgacat cacgattcgc
     3361 ggctgaagaa aatccttttc agtctgttca aatacttcaa taggtgcttt aaattgataa
     3421 ttggcaggag ttgggtaaag tctacccata tctgccattg tgccatcagc gtttacccta
     3481 tatggctgca agtctcccgg tgacgaaagt atcagtctat caatactatt cggaaaacgg
     3541 gcagcaaagt gaacagccag aatacctcca aaagaatgtc ctaacaatat aactttgtca
     3601 gacatgatgt gtgaattgat gatttctttc aggtcattca aatgcctttc aaaggaataa
     3661 tcctttggtc gggagagtcg gtctgataat ccggagccaa tttggtcata cagatagaca
     3721 tcatagcctt ttttggcgac ttcatccatc tgtttaatga ttgctgaatg aacataacca
     3781 ccgggtccgc catgtaaata tattattgga gggttgatat tggcagagtc accctttaac
     3841 aaggaataag caattttgga gccatcccgc attgtccaat atttcgtttc agcccgcttt
     3901 tgaataggac gaatggtttg gcggattgga atcatactcc aggtgagaaa tatcagaatg
     3961 attattccga tacctcgtaa aatatatttc gcaatttgtc tgagaataga atttttcttc
     4021 tttttcatca actgtttgaa tacttatttg gccagctaat atttttggtt tcaaactttc
     4081 tcttttatcc ggacaatcca ctttctatgg tacaacctta tacttaaccg tgtatttgct
     4141 gttatccata ccgacattat acaagtttag gacaaacagc cttgtgttat tgattcaatc
     4201 tcggtcttct tccacaaatt ccaaaatatc cccgggttga cattccaata ctttacaaat
     4261 ggcttccagt gtgctgaaac gtatggcttt ggcttttccg gttttcaata tggaaagatt
     4321 cgaaagtgtc aatccaactt tttccgacag ttcattcagc gacattttcc gcttagccat
     4381 cattacatcc aggtttacaa taatcgccat atcttatact gttaaatcat tttcgttttg
     4441 aatagccacc ccttttctga aaatcactcc aatgatataa accactcccc ccattaatat
     4501 atatgcatca ctatcctccc aaaacgggcg taatttgtca gtcacaagcc catgatgcat
     4561 catggttttt gtcacctggt gagctaaata acttaaaaat cctataacaa gggtgtaata
     4621 actgattaag gcaatttgct ttgcaacaaa gtcgctgaat ggcttggaca tatccatagt
     4681 atgcatcaat ctgatcacag tataaaataa acacgctttt aatattgcaa tggtcaggat
     4741 aaagctatag taacagaaga aggccaatct gctctctttg tatatttcgg ttaagtccag
     4801 cttttggtat aggctttgaa tcaattctgg cttataaaga ctgatgataa aattgactaa
     4861 taagcctcct gcttcgatgg acaggcctac aaatatgatc caggctactg aataaagggc
     4921 ccagaatacg aagttgtttg tttttgacat tggtaatttt attttgattg atgatgcaaa
     4981 gataataaat aattattgat aaacaataaa aataaaataa aaaacaataa aattttatct
     5041 ttattccaga tggggtgtcg atcgacggga gctatttgca cggttgccat caagtgaacc
     5101 aaaagaaaac acaagatgga tagtattatg gattgagggt cgatccatat aatcctttta
     5161 atacctaaaa tttatggact attatataaa tcatcttggt ataagataag attttactta
     5221 ttctacgaca tttttaaatg gtaaaaagcc ggacaacagc attgtgtagg tgtccatgcc
     5281 ttccaggttc ggcatgtcgt gtacacttaa gcatttctga tgggttaatg ctactgattg
     5341 ctttggaggg aagattcaag tatatgggaa aatagcggta aaattgaagc atttctaccg
     5401 tccttcttta atcagcctta tacgattctg ttcaaatcca tctcctgaaa atttcaggac
     5461 gtaagtgttc cggacaatgt ccctgttttc ggtgttttga ttctcatgtt gtaatttagt
     5521 caatattttt ttcatttacc tacccaaact ttttggcaaa taccgaacaa accacattgt
     5581 atctccaccc ggcatattcc aggtgtcata atggatgaat ttatagcgct gataaaccat
     5641 ggcattggta cggagtcggg tttcagcgta gatggggagt tgctcatcct cacttattcg
     5701 tagaaacata tctttcatgt tgactgcgac tcccttgccg gctcccctgc tgccggggat
     5761 cacaccccaa aaccacatat agaagaatgg ttcacttttg ggttgtcggc tgcggatgtg
     5821 tttccgtacc cggatgattt tcgggacatt ggtaagccca atgacacaga aagccagctt
     5881 gatatcttcc catacatccc ggaagaagtg attccgaccg gcattcattc taaacatcaa
     5941 tgcgataccg gttcctgtat catctgtaag taaggcattg tggcggattg ccttttcgat
     6001 catataccgc gcaagtaagg taaatcgcct gtccctgcca ggtccctgcc ggaccgtatc
     6061 ctgcgcggtg cgtgtttcct tcagcacttg ggctacctgt ctgatgattt cttccttgtc
     6121 aaacattcgt tggatcgtat tattcaaaaa tacacaagaa ttttcagtct aataagtctt
     6181 cgtcatatcc tgtggcatta ccagattgaa ttctgttttt ttgaagtctg agggcagctt
     6241 gagagcagga tctgccgact ccgatatgga gattacggca atcttcagtt cgggtcttgc
     6301 cttcttgagg taccagtata taccgccatg ttccttgcta tgaaagtcac cttcatagtg
     6361 gaagaagagg gtccccggtg aaagatggtt taggatagat tcagccatcg tcgcatcctt
     6421 gatggcctgt gcagcgacaa aattcatggc gcggctgccg gcatggtcac ccatcatttt
     6481 cagcatctcg tcgtagccgg gtgtttccat actgacttca tagggcagct tgaccaccca
     6541 aggcacttca tgtgactcca acttcaggct gtccttgcct ttttttgcga ccagggctgc
     6601 aaaacgacgc ggtacattgg tggctatcat cagaagcttc ttttgcctgg caaattcgag
     6661 gattggcaag taatccgtct tatgattatt ccaaagtcgg gcttcttcac ccagcttttc
     6721 atcgttgatg tcaccactga gatatcggtc tatcgcctcc tgattgtcgc gttcgtacat
     6781 ctcacaaccc agaatgagcc ggtttccttt cttttcaaag agtgcttctg ctgttttcaa
     6841 ctccagccaa tgattgactg aattgttgtg atactcgcca aagagtacga catcgtaatc
     6901 cttcaggctg ttgatcatct taccaaaagt gacggccttt ccacgggaat tatagaattg
     6961 gtatggtttc agctcctggg cttgcaccaa tgccggaaaa atcaacagca gagacaggaa
     7021 aagatttttc attggattga cgatactttt ttcaaaaata gaaataataa ggatagtgtg
     7081 ggtaaaatat gtcattctaa ttggttttga agtcgaacag gaagttgatt tcctgtttga
     7141 tttcaaaatt cctgatttga tttgttgaga cagctgagga tataattgcg gcttgtcctg
     7201 cgacacaatg ccttttcgga ttccgctcca aagaaaaagg tgacctctag agtcagccat
     7261 acatttaagc ccaactgtaa tccggtcaga taaattggta ggtatgtaat cttttacagc
     7321 atcatcccaa gtccgatatt tcgattaact ttatgtttta aattgatgga gggatatgca
     7381 tttttatgaa aagacggttt taataacggg tggctgctcc ggtatcggta agattatggc
     7441 acgcaaatcc cttgaaagag gcgcatcacg tgtcattatc attgatgtga atgatgcggt
     7501 atttcaggat ttgaagaaag aatgggaagc cttttccggt gaggttatca ccttccatgc
     7561 ggacctttcc aaagtggacc aaatcagaga tgtggctgca agagtcaaag aagcttatgg
     7621 agtgcctgat gtactgataa acaatgccgg catagtcacc ggtaaatatt tttacgaaca
     7681 tagtcatgaa aatatttcac tttccatggc agtcaatgcc gatgctatga tgcacctgac
     7741 actcgaattt cttccgggca tgatagagcg taattcaggg tctataggca atttatgttc
     7801 cctggccggc ttgatcggca atacgaaaat ggcggtttat gttgcatcaa aatgggcggc
     7861 cgtgggctgg agtgagagtc tcagacttga aatgcaacag cttggcaaag atatcaaggt
     7921 gacaaccata atgccttatt atatcaatac cggcatgttt gacggtgtcc ggtccggcct
     7981 gattcccatt ctgaaaccag aaccatcgtc cgaaaaattt atacgggcca ttgaaaggga
     8041 aagaaagttg ttcgctacgc cggtgccgta ctggtttatt cgcctgagtc agggtatatt
     8101 gccggtttca gcatttgatt ttgtgatgaa gaatatcttc ggtgtttatg acaccatgaa
     8161 gaaattcaaa ggtagagacg gaaattgaat tttgtttatt ggctccagtg aataaaaggt
     8221 gtcggattat taataacctt acgaatagtc cgactttttg aaggtaagac catggaatta
     8281 tgcttgttgc tcccgggcta actttcatcc cttatatgcg acaacttttg tccatacctg
     8341 ataagggaca tagattaacc taaaatttcg atctgtgtat tgttacgagt ttataaaact
     8401 tttgagccgg gtgtttgtcc atgggcacag gtataaccgg aatattcagc cgaaaaaatc
     8461 cgattcaaga caaagtacag aagcgggagg gcttgttgcg ccctatagtt tttagtaaag
     8521 gggaaagagg caagaacaat gtaggtacag gagtcaacct aataatcact ccttagacag
     8581 tcgaattgaa tgggccggaa ataacaggta tgaatgttga aaattggttt gaaaagttat
     8641 tgagactgct catcaaatct tagaaaaata tatgaggtgc accgttgata aacatccatc
     8701 attggatata cagacataaa cgttatattt gattgaaaaa acagagatgc acaggatcag
     8761 cgcttggatt ttgaaaatgt tgggttggaa gattgtaggc tgggatatga atgctttgaa
     8821 aaagtatgtc ctgattgcca tgccccatac ttctaatctt gacttccctc ttggtttgct
     8881 ggtgcgcagt tccataaagc tcaagataca ttttgtaggt aagtccggtc tattccgctg
     8941 gccatttggt ttcatattcc gacgtctggg gggatatccg gtggatcgca gcagaagtca
     9001 caattatgtg gaagcagtag ccgccatgtt caaggacaaa gacttgttca gactcaccat
     9061 tgcgcctgaa ggtacccgaa gcaaggtgga ccggttgaag actggttttt attatattgc
     9121 acaaactgcc cgtgtaccga ttgtcatggt tgcatttgac tataagacac atgaaataag
     9181 ggttcatcca ccaattgact ccagtatttc ctatgatgat ttatatcaga agatgaaaga
     9241 attctttcgg ggggtggaag gtcgacatcc ggagaaggat tatgagtttg agtagaaatc
     9301 cttttgatga tgatttgctg aaatcaatcc ttgaggccgg catggtagct tggagcgtta
     9361 tattggtagg aaaaagatcc ggatacatca tgtgttaaat tttctggtag ccggtttatt
     9421 ctccgtacat tcgacctttc atattaacca aaaatcgatg ttcagaataa tcccgattgc
     9481 actattttgc ctattggcct acatttccaa tggccaaaca gcttctgaag acaagatcaa
     9541 tactgcaaat aaggctcttg cattcctgaa taagattgcc gatatgaata agtccgggag
     9601 ccgcgctttt accttgaagt ttgatacgtt gtccattttt tttgcagggc ctgaagagcc
     9661 ggaatcctta gtgtccacca tgccggtgac ttatgatgca tggggaagaa tcaaggatgc
     9721 aaggattatt gaagcgggct ttgaagatta ttaccaatac agatacaatt ataccggtga
     9781 tgacaacaga ttttcacatc tggactatta tctgcattcg ggtggtgagg agcattatgc
     9841 cggtgttcag attcatcttt atgatgatca tggacgattg atttcttcca gggaactgga
     9901 caaggacaat caattgatct atggtgattc gttgagctta gaattcaatg gactcgggca
     9961 aatcgtattc tatgaatatc tttattttca actgggtaga tggaactctg gtgggaaagt
    10021 ttatgatgtc caatatagtg gacaagatat tgtaggatat aatgccgatg aagcggaaag
    10081 ggggcattcc gggaaggata cgattgtctc gtaccgatac gaggatgtca ggtttttaaa
    10141 tgatgactca tccattcttt ttcctgattt tgatgaattt tttgaaattg actcgttagg
    10201 cgttgataat ttcttcctga gcagcaggga tgccgccaag gcatatgtca aacaaccggt
    10261 gcaatacctt aaatacagga cagatggttc aggagccttg gttcagtctg cagtgtatga
    10321 tccggataaa tttatttcca tcatcagtga tccgaacgga aaggagatat acaggattga
    10381 atattctttt gatggtcagg gaaggctaaa ggggattcac aaggtagaaa ccggacaagt
    10441 accggaagta tcgacctttg aatataatga attggggcgt atggctcgct atattttcac
    10501 atcggattct gttaatttca gggttaataa tctctatgtg acagatgata aagatcgact
    10561 cagtgaatac tatcaaaatc tggaagatac caacaattcc attaccaata tactaaggtt
    10621 tggttatagg ggtgagaata aaacatatac aggaagtatt ttggaggcgt gcgtatttcc
    10681 gaatcctgcc gggcatgcca tttatattaa aactccgcaa tcgatttatc aggagatgca
    10741 ggttagtatg atgggaaatg atggtagatt ggtattttcc ggaagaataa atttcacagc
    10801 cggggtaggc tcgatccccg tggctaaggt gcctccgggc acatatttac ttttgataaa
    10861 ggatggaaaa aaagcctaca aggctacggt ggtaataatt taacgagggg actcgcacta
    10921 tttttattta taatttcata aattgcaggc attatccggg aagtttcgga tagtttgtgt
    10981 agcattggtt tgcgaggagt tggatatatt ggaacaatat tttctgtcaa ataaaaccgg
    11041 aaggggagcc ttcacaccac ttttatgaaa cgaaccaaag ggaatctgat caggtcactt
    11101 atggccggcc tgaataaagc tgtgcatgaa tatcaactgg agacctcatc aaaagaaggt
    11161 gttgcaagat acaaccggag gacttttatc caacagtcca caaaaggagc aataggcgtt
    11221 ggtgtcgctt tgagtctgcc ggcaatgctg atttcatgca aacaggagac acggcctccc
    11281 caaggagtag agcaggaccc tgtcctcgat gttgcaatcc ttggaggagg gatggcggga
    11341 ttaaactgtg ccaatcatct actgggatca gggcttacgt tcaaggtgta tgaaggaagt
    11401 aagagggtcg gcgggcgaat cctcacacat tataacgact ctatgcaatt gggaatattt
    11461 cctgaatttg gaggtgactt tattgattcc gaccatgagg atatgatcaa acttgcgaag
    11521 gagtttaatc tggacctggt ggatcagata gctgaacagg aggctaatca atgggtaaag
    11581 gatatttatt tttttgacaa tagaaaaatt tctgaaaagg aaatcatccg tgaatttaag
    11641 aaaatagcac cgaaaattgc aaaggatagg gatgcgcttg gcgaaaatta tgacacgcct
    11701 gaagcaagtc gaatggataa gatgtcgttg tctgaatatc tcgattcact gaaatgcaaa
    11761 gaatggatga aagagttatt ggatgcagcc tttgtagccg aatttggatt ggattgtagt
    11821 gaacaatctg ccatcaatat gctggatctc atcataacag acacatctga aggatttaaa
    11881 gtgtttggaa ccagtgatga gcgttatcgg attaaaggag ggaattcgca gatcacaaac
    11941 aagctggcag agaagatcgg tgaggacaat attctcaaaa actataaggt aacggagata
    12001 tcagaaggag ataacggaat gtataccatc gtctttgaaa atggagagaa ggtcgaagca
    12061 aaagtaattg tatgcacaat cccctttaca attcttcggg aaataaaact ctcactaaag
    12121 ggaatgacac ctgaaaaacg taaatgcatc gatgagctgg gttacggtat caataccaaa
    12181 ttggtgctgg gttatgaagg tcagccatgg cggagtaagg aaaataatgc aatggggtac
    12241 ctgtttacca aagatatgac caatggatgg gatgctagtc tgaaccgcac ggagaataac
    12301 gagaatggtg catatgtggc ttttttcggt ggcaagtttt cgcagtcgct ttgtgatgaa
    12361 tcctcgcgaa atcctatgtc cccaccggaa catgtttggc gtacaatgct accagaaaag
    12421 agggtcaatg ggtttgtaga tgaccttgac aagattttca ttcgctcgaa aaagaaattt
    12481 aaaggaaagc atgtatttgt caactggatt gaatatccgt ttgtaaaggc gagttatgcc
    12541 tgttataaaa ccgggcaatg gacaacgatt gccggtcatg aggcaaaacc ggtcggtaac
    12601 ttttattttg ccggtgagca ttgcagtaac aattttcagg gatttatgaa tggagctgct
    12661 gagagtggca gggaaacagc aattcagctc attgaaaaat atgctaaagt tcctgtaaac
    12721 taagatatga gcagctatca ggcaatgtgg ttgtttgttg gtgacattgt aattattcac
    12781 ctctactgat atcggaatac aaattgaaat taatattacc caggagataa agtccgcatg
    12841 cttttattca ttaagccttt aatgatgcca ggaagtcgga aagtatccgg tgttaatgaa
    12901 agaaatattg gctatgacgg ataaggttac cggtctgaaa agatatagtt ttgaacggaa
    12961 tgattctgtg gtatttaatt catgatggca ggagtcgcaa gttcaaaagt cggaatatcc
    13021 acttcaaaaa gattacctga atgcagattt tccaaagtgt aggtcccaga catagtaccc
    13081 agttcggtac ttaaattgca gaagcttgag tagtcgaaga tctgatcggg ttcgatgatg
    13141 ggttgcagtc ctaccacgcc ttcgccatcc acatatcgca cttctccatt gctatcaaag
    13201 atgcgccaat gcctgctgag caactggacc ggaaagcggt tatgattacg gatggaaata
    13261 tggtaaataa aaatgaatcc tgcccgtccg tcagtatccg actctgtatg aaaatccact
    13321 ttgatatcga cattcactcc tttagtagta agactggtca tgttgttgct gtatttgaca
    13381 ttctttgaat acctatttcc ggatctgttt agttccggct atagtttcac tgcaaaaacc
    13441 attcctaaaa gtcatgattt aacaaaaagt cctgacaaaa gacttgtata ttttcatggt
    13501 atggattgaa gagtacatca tcagggacct tcctcacaga aaattttttt gctgtcactt
    13561 acggccgaag tcatcttgca accttacgat atcctcttca ttacttggat tggatgcgtc
    13621 ggtatgctgc catatttctg caatgattcc gtaatcatca ttgccgatga tacgatgcct
    13681 ttcgccctgg gcaaggcgaa tgatatcgcc gctttccagg attcgggaat atttttcttc
    13741 atccgtatcg cttgtgatta cggtgatatc cccatgaatg caccgccaaa cctctgctct
    13801 ccgaaaatga tactgccagc tgagacgttg acccggggcc acaacaagca gtttggggct
    13861 cagggtggca ccttccggaa agtctttaaa gtggatctca gtgcctgagt aaaaatgcat
    13921 tataaacgct tcagcaaggt tttcctcaaa agtaaaaaag ccaccccatg gacgggagtc
    13981 atcaatactt ctgaatgcca agccaatgga agcaaggtaa gatttgacag agtcaaagac
    14041 actacttttc gattcggtat caatattcat catggatata tactaacaaa tatttaatta
    14101 aaaaagtgca agataaaaaa agacgtaacc tttcagcttg tgtaaatcga aaaactttac
    14161 cttaaaaaat gaaaaaataa aagtcaaatt gcaaatcgac agagaacttt catttacaat
    14221 acattatttg tttttataat aaaaaaaatc aacaatatat cttatctttg cgccgagaat
    14281 tctctttaga atatcataca gtattatatg aaaaaatttc tgacaaaatt tggtaggcca
    14341 cttcttttcc ttacgctttc tctttttgct atacaatgta gtgaatcaga ttatgatatt
    14401 gaaccgcaat ataaggatgc gtctcacttc gaaggcgatg tagctataga atggaacagt
    14461 attttgaatg aactggatcg ttatacggaa ggttacaaat cgccgatagc tgcacgtgcc
    14521 atggcatttg tcaatcttgc agcctatgag gctatgatac cgggaatggc cggcaagtat
    14581 atatctctgg ctaaagacaa ttttcgtgtc tcaatacctg cacccgaaaa ggataaagct
    14641 tacttttggc cattggttct gaacacatgc tataaagttg cgattcagaa attctatccc
    14701 actgctcctt ctgcacaaca attggccata ctcgatactt atgagaaatt gcaaaagaaa
    14761 tactctgaga aaatcagtcc ggagactgtt cttcgttcct tagagtatgg cggcaccata
    14821 gcgggattga tttacgactt ttccacaaag gatgaattgg ctgatgctgc ttaccaacgt
    14881 gtaaatgacc cgaattacat tccgccagca ggcgaaggta attggcagcc atctccaccg
    14941 gattttgcgc ctgccgttct accttactgg ggaaatgtca ggacattggt agctgtcgat
    15001 tccgatttct ggatttctgg gccacctgaa tattcgactg tgccacaatc acgaatgtat
    15061 aaggagacca agtatgtaca tgaccgcggc aactttttgc gtgacaatcc ggaaaatgaa
    15121 agtaattgga ttgcgcaatt ctggagtgat gatttttctc cgttaacttt cagtcctgcg
    15181 agccgttgga atgcaatcac tacacaaata ttgcaagaaa gggagagtaa tttggaagag
    15241 gcagtcatga gctatttgaa ggtatctttt gcactttcgg atgcggcaat tggttgctgg
    15301 cagaacaagt acaaatataa tgtaggtagg cctgttactt atattcgggc caatatggat
    15361 gctgactgga atacactcat gaaacctgga ttggaagaag gattctatac accggctaca
    15421 ccatcctatc catccgaaca tgcgacttat gcagcggctg cgacgggtgt attagccaac
    15481 ctgtttggca atgaatgcaa ttttactgac cattgtcatg aaggaagaac agaattcaag
    15541 ggtaatccgc gtacatttta caaatttagt cagatggcgg aagaaagtgg attttccagg
    15601 acacagattg gtgtcaatag tcagtttgat gtcgatggag ggttacaatt aggtgcacag
    15661 gtagccaagg ctatcaatga cttccgtttt caaaaataaa aaggccaatt aagataaatc
    15721 aatggtgatc attccggtca ccattttttt tttgcggatt gtcgggtaca ggatcaaatc
    15781 catggccacc ccatggatgg caactcatga tgcgtttgaa tcccagccag gaccctttca
    15841 atacgcccca ttcttcaata gcctgaatgg cataatgcga gcatgtcggt tgatatctgc
    15901 acttgttggt tcccaagagt gggctaattg aaagttgata gaagcgaata ggcagaatca
    15961 gcacatattt gagtagacgg ttcaggtaat acataatgaa ggaacaatgg aaaaataaaa
    16021 ttagtttagg gatgcatgta atgggagaat atggcaggag ttggggtgaa ggagaaaagg
    16081 agtgaggggg gatggcgtct ctgaagtagg tagatgatta atagccacaa tgattgagag
    16141 tattgccttt tggggtgaac ttgctaaaat tcagatgatt tactcgggaa ataaattcct
    16201 catttgtccc caaaatcttg tataaagcgg tttttgaatt attactttcc tacctacttc
    16261 gatttttggg gaagttgtgg gtggtgccgc ctccgaagta ggtagatgat taatagtcac
    16321 catgcttgac ggtattgcct tttaggatga ccttatcaaa atccagatga tttacccagg
    16381 aattaaattt ttcatttgcc tccaaatcct tgtgtatagc ggtttttgaa tta
//
LOCUS       DAOQMC010000021        15744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1351_length_15744_cov_4.587099, whole genome shotgun sequence.
ACCESSION   DAOQMC010000021 DAOQMC010000000
VERSION     DAOQMC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15744
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1351_length_15744_cov_4.587099"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2460
                     /gene="gyrA"
                     /locus_tag="PKV31_01685"
     CDS             <1..2460
                     /gene="gyrA"
                     /locus_tag="PKV31_01685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNB91591.1"
                     /translation="QRIVPINLDDEMKTAYIDYSMSVIVSRALPDVRDGMKPVHRRVL
                     YGMDGLSLNYNKPHKKSARIVGEVLGKYHPHGDSSVYFTMVRMAQEWSMRYPLVDGQG
                     NFGSMDGDSPAAMRYTEARFSRIAEELLRDIEKETVDFALNFDDTLEEPTVLPTRIPN
                     LLINGASGIAVGMATNMLPHNLTEVIDGLIHFIEKGELSVDEMLTYIKGPDFPTGGII
                     YGTSGIREYFATGRGRIVVRGKAEIENSGNKETIIITEIPYQVNKADLVAKIAELVNE
                     GKIPGISDTRDESDRNGLRVVVDVKRDAMASVVLSMLYKYTPLQTSFGVNNIALVKGR
                     PMLLTLADMMRHFIEFRIEVIVRRAKFDLRKAEERAHILEGLLKALDHLDEVIALIRA
                     SKTVDDAHSGLVARFEFSDIQAKAILEMRLQRLTGLERQKIQDEYQELLKTIAYLKEL
                     LQSEQMQKDVVKGELIEIKEKFGDARRTEISHAEGDISIEDLIADEAVAITISHLGYI
                     KRTSLDEYKPQKRGGRGSQGSKTRDSDFIEHMFVASNHNYLLLFTEQGRCFWLRVFEI
                     PEASKVSTGRVIQNIINLPQDDRVKAYIPIKDLNDRDFLDNHYILFCTRKGTIKKTPV
                     SDFSRPRSNGINAITIHDGDMLLEARLTNGDNEIILANRHGRAIRFNESKVRSMGRTA
                     AGVRGMLLDGPDDGIVGMVCVNPNDMLVTIFVVSENGNGKRSALEDYRVTNRGGKGVK
                     TMRISEKTGNLIAIKEVYEMDEMMITTKNGVIIRMAMEALRVMGRATQGVRVIKLDSG
                     DRIADIAVIRESMLSDKTEEE"
     gene            2617..3816
                     /locus_tag="PKV31_01690"
     CDS             2617..3816
                     /locus_tag="PKV31_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91592.1"
                     /translation="MKNLLWILLMVTIVNISASAQDDGAKLVKKAKKAITNYFLDPSN
                     KDNLAESQQLIEKAFATGTVDNDAAAWATKGDIYAELAKADAAIKMIDPNVKLKNIEA
                     PFIAFESYKMSIAKATKESAKKDANNGLNELIPMLSTSGLEYFYDQNFEKAFRDFKAI
                     IEAYDIAKSAKLKTALDKPEDLNNQYYVTGLSALNAGKEAEAGEWYEKAFNGGNKGAE
                     IYDGLFKSYQKSDPAKAEKYLTEGRTAYPDDNSLLFSEINLYLSQGKLSSLIDNLELA
                     CQKEPDNISVINTLGNVYDKLYQNASEKGDAQEAENYFNKAMESYNKALAKDPSNNFA
                     NYSMGTLYYNKAANYVKVMQTLSDDLSKEGMKKYDEAQKNMFGMFDAALPYFLKAEES
                     DLKIETP"
     gene            complement(4037..5371)
                     /gene="tilS"
                     /locus_tag="PKV31_01695"
     CDS             complement(4037..5371)
                     /gene="tilS"
                     /locus_tag="PKV31_01695"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNB91593.1"
                     /translation="MNSFLRNILQRFNKNLEVLAGNEYTRFKYLVAVSGGSDSIALSE
                     LCRMSDLDIAIAHCNFKLRGKESDEDEAFVVEFATGHQIPVFTRTFDTKLEARLLDLS
                     VQETARKLRYDWFYQLCESNQFDFILSGHHEDDRIETFVINLMRGAGPKGLRSIPPRN
                     NKILRPLLGFQREELKVFCHHAKLQFRSDSSNLDDKYSRNFIRNQVIPLVENRYPGMK
                     GSLTHVMKIQDLYFRFVETRVEKELHQRIKPNPYGMILDLNDLSEPDLLIFLLIFWMG
                     DLGFSHSDIEDMSRCLSERKSGARFFANNSMVISDRGKIVYLDREVPSITLEFPLVEG
                     KYQYHDSIFVISKSNRIPDKGEIKIPEKAKQSTIKIRARQDGDRIRSLGMEGKSQKLQ
                     DVYTNARLNYLQKLIQPVVLLDDKIIWLPGLKKSVETSTADGPGWIISHKSH"
     gene            5553..6134
                     /locus_tag="PKV31_01700"
     CDS             5553..6134
                     /locus_tag="PKV31_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91594.1"
                     /translation="MINFTLRLLTFCLFLTFIQCKPGDNGTSSVEATPRTDSVRFKKY
                     VPEPGDVPVDPEADDNAFRPQLPLDQAGFKSAKPDEARRLENEFEFMFDKEWKINGRI
                     YANDDKVHRKGQEDFRFNRDGSYSGVVEGKEISGTWMGRLENNAPVITVFPTDLKEKV
                     NEWFVKNTGKTMVWSGTPSYKDNGVMIQFVLKK"
     gene            6189..8507
                     /locus_tag="PKV31_01705"
     CDS             6189..8507
                     /locus_tag="PKV31_01705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB91595.1"
                     /translation="MFSRNLQSLTLFVFMMGIFSIAHSQTDNTIFKVEKASLSRDVLE
                     YSPLYLKGDWVYTQLDDKNVNIYSLGSDKVIPIEGFSLRRAIVTGFAKSDQGGLIAIS
                     YGDNKQNVPAKLKGNKIYFAFQDKNGIASKYFEFQLNSEAYSCTTPYLSPDGTKLYFA
                     SDMPGGYGGLDIYVCDYRGKEWSKPRNLGPKINSEKDEVYPYMIDNMLFFSSNGHGAN
                     RMDIFIADMDKDLGRLVINAGSPINSPADDYSMSFDNNTRIGYFLSDREGVNGKAFKV
                     TNEKKLILLNIKSNEDKKAISGAKVDLSRCRKNPLYANSKGSVILPVAAGEKCFIQVG
                     KVGYNSATIPIDFDEIIGLKKSININLSKEGVFYQGRVVDQDNNPADEVELSIVDQAT
                     GELQYVFTDDNGYYTIALEPLSYYMIRSKSPYYNPSESKIVTLASVPADILGTIKLVS
                     NGSRRSGIIKTSSMDDIKVTQRNESETENPSTSDFVETGVAKKIESVKGPETSVTDAQ
                     KVMDYNDNRLREETVKEEVMQENKQIAPAQSPVIVAPKTENKKEAIVKYAIQLAAISL
                     DNHNLQPYQEKTRGRGQVYIAREGNLNKVRLGFYETRQDALAIREQLPEELRKGFIVE
                     VKESEYNNRQAIPVDRKNAIDPPMNKPEKKAEPDPRIQPENKPEMKSEVKPQEKTNSE
                     PQVVQKSLEESRVEYKIRLSTLRNASLFNGSAVRQYGLIEEIKTGNLTTFYLSGFENK
                     EEVKQVIDKVKAGGFPSAQIVKLTNGEYTIDN"
     gene            complement(8575..9150)
                     /gene="folE"
                     /locus_tag="PKV31_01710"
     CDS             complement(8575..9150)
                     /gene="folE"
                     /locus_tag="PKV31_01710"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNB91596.1"
                     /translation="MDTNEYQDSRIDNLINHYNQIITLSGEDVEREGLLKTPKRAAKA
                     IDFLTSGYRMDAAEILRSAMFNEPYNEMIIVKDIEVYSLCEHHMLPFFGKAHIAYIPK
                     DYIVGLSKLPRVVDVFARRLQVQERLTQEILEVIDNTLKPHGVAVVIEASHMCMMMRG
                     VQKQNSVTTTSAFTGEFKNVETRNEFLQLIR"
     gene            9672..10280
                     /gene="pnuC"
                     /locus_tag="PKV31_01715"
     CDS             9672..10280
                     /gene="pnuC"
                     /locus_tag="PKV31_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNB91597.1"
                     /translation="MEILEAVWQYFQSHTLEFVAAFFGVVSVWYSRKNDILVYPTGIV
                     SVLLYVYICLIHKLYADAGVNFYYFVMSVVGWVNWSKKKEEEFVFPISYCDRNEWIKG
                     IAVFLIILISLLFFLFYFTDSTTVVGDSLVSASAATAMWWMAKRKIESWYAWILSNIV
                     ALPLFFVKELHFTVVQYIVFLYLAVWGLITWRKEFKKGYAKL"
     gene            10267..10887
                     /locus_tag="PKV31_01720"
     CDS             10267..10887
                     /locus_tag="PKV31_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB91598.1"
                     /translation="MQNCDCSVTDMIDIGDQPFTRVPPTRAGNMFAVVGVESTGKSTC
                     AAFLTEALNGKLIEEYARLYLEEHSNTYNHEDFLEIAKGQAYMENHAMETAAEGDILV
                     FDTDYIVIYVWSMIVFNNIPQWITNRIEHYPPKVYFLMTPEVEWVNDGLREYPDLEVR
                     ESIHRIYIEVLEKFNCKYFIISGPDYRARQEKALQLALEEINKAKG"
     gene            complement(10914..11483)
                     /locus_tag="PKV31_01725"
     CDS             complement(10914..11483)
                     /locus_tag="PKV31_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNB91599.1"
                     /translation="MDWLIPILFGIGLSAATGLRVFLPFFLISLTFQFDLIQPNNEFL
                     WLDNKYVALALGIATSLEILGFYTPIIDHFMDIVGMPLVLCAGFLSMFSVLPEMPFHA
                     EYLLSLIGGGGTALSINSLTGVMRIKTTASFYGLANSTYSTLENILCVCFLIFAFLIP
                     IVFGILIIMTAFFSFRVIRNFARKILKLN"
     gene            complement(11487..12725)
                     /gene="rocD"
                     /locus_tag="PKV31_01730"
     CDS             complement(11487..12725)
                     /gene="rocD"
                     /locus_tag="PKV31_01730"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNB91600.1"
                     /translation="MLTKERSATTFITLEDQHGAHNYHPLPVVLTKGEGVYVWDVEGK
                     KYYDFLSSYSAVNQGHCHPRIIEALVNQASRLTLTSRAFYNDKLGEYEAFMTGLFGYD
                     KLLPMNTGVEAVETAIKLCRKWAYEVRGQKSGTAKIIVAEGNFHGRTMAAISISSDPD
                     SKGGYAPYLPGFVEVPFNNIEAFKKAVEDDEVAGVLMEPIQGEAGVRVPDDTYLKEVV
                     SICKEKNILFIADEVQTGIARTGKMLACDQLGIRPDILILGKALSGGVLPVSCVMADD
                     EIMLTIKPGQHGSTFGGNPLACAVAMEAMQVVLDEDLAQKAEYLGRIFRAEMQKLVES
                     SDLVKLVRGKGLLNAIVINDSEDSSTAWDICIQMAENGLIAKPTHGNIIRFAPPLVIT
                     EDELLDCCRIIRETIVNFSK"
     gene            complement(12888..15740)
                     /gene="gcvP"
                     /locus_tag="PKV31_01735"
     CDS             complement(12888..15740)
                     /gene="gcvP"
                     /locus_tag="PKV31_01735"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNB91601.1"
                     /translation="MNFEESKFEDRHLGLRSEQIAEMCSVIGVETLGQLVDQTVPESI
                     RLQRELNLPEAMSEHEYLEHVKGIASRNKVFKSYIGQGYYNTIVPNVILRNLFENPGW
                     YTQYTPYQAEISQGRLESLLNFQTMISDLTGLPIANASLLDEGTAAAEAMHMFYAFKN
                     KSGDEFNQFYVEKGVYAQTLDVVKTRCKVLDIEVVEFDRSTDRIGDKAFGVLMQYPDK
                     TGGVHAIGDDIRALKERGILVAVAADLMALALLTPPGELGADVVLGNSQRFGVPMGFG
                     GPHAAYFSTGEEFKRAIPGRIIGVSIDAHGNRALRMALQTREQHIKREKATSNICTAQ
                     ALLANMSAMYGVYHGPGGIKRIAGNMHSLAVTLNNALTTAGMKTLNNVFFDTIALSMS
                     SEEIAALKRKSEAMSINFGYLDEGVVVIALDETTSLADIADILNTFDIHRTTEQLKAD
                     MQSLNSPITGELVRTSDFMTHEVFNSYQTESKLMRYLKMLENKDLSLVHSMISLGSCT
                     MKLNAASEMIPVSWPEFANIHPFAPRDQWAGYYELIKNFSEYLCEITGFTGCSLQPNS
                     GAQGEYAGLLAIRRYHEANGDHHRNISLIPSSAHGTNPASAVMAGMKVVVVKTDDEGN
                     VDLEDLRALAVQHRDNLSCLMITYPSTFGVFEVSVKEICQVIHDNGGMVYMDGANMNA
                     QVGLTNPGEIGADVCHLNLHKTFAIPHGGGGPGMGPICCNDKLKPFLPGHTKMGDDFA
                     VSSAPYGSASIILISYGYVRMLGGSGLKKATEIAILNANYIKARLEKRFPILYSGAQG
                     RAAHEMIVDLRPYKHLASAEDVAKRLMDYGFHAPTLSFPVAGTVMIEPTESESKDELD
                     RFCDAMLNILAELEEIADGKAAADNNVLVNAPHSLPLVTANEWNLPYSREKAAFPLDY
                     LRHGFKFWVGISRVNNAFGDRNLVCTCPPIEDYINS"
BASE COUNT         4627 a         3166 c         3434 g         4517 t
ORIGIN      
        1 cagaggatcg ttccaattaa tctggatgac gaaatgaaaa cagcatacat cgactattcc
       61 atgtcggtta ttgtttccag agcgttgccg gatgtaaggg atggcatgaa gccggttcat
      121 cgaagggttt tatatggtat ggacggtctg agtctcaatt acaataagcc tcataaaaag
      181 tctgccagaa tagtgggaga ggtattgggt aaatatcacc cgcatggaga ctcttctgtt
      241 tactttacca tggtcaggat ggcccaggag tggagcatga ggtatccttt agtggatggc
      301 cagggtaact ttgggtcgat ggacggtgat agcccggctg cgatgcgtta tacggaagca
      361 agattttcga ggatagccga ggagttactc agggatattg agaaggaaac cgttgatttt
      421 gccctgaatt tcgatgatac gctggaagag cctactgtgc ttcctacccg tatacctaac
      481 cttttgatca atggcgccag tggaatcgct gttggtatgg ctaccaatat gttgccgcat
      541 aatctgactg aagtgattga tggtttgatc cacttcattg aaaaaggtga gttatcagta
      601 gacgaaatgc ttacctatat caaaggtcct gattttccta caggtggcat tatttacggc
      661 acctccggta tccgcgaata ctttgccaca ggccggggcc gtatcgttgt cagaggtaag
      721 gctgagatcg agaattccgg aaataaagag acgatcatca ttaccgaaat tccataccag
      781 gtcaataagg cagatctcgt tgccaagata gctgaattgg tcaacgaagg taagataccg
      841 ggcatctccg acacgcgtga cgaatcagat aggaatggtc ttcgcgtcgt ggtagatgtc
      901 aagagggatg ccatggcgag tgttgtattg agtatgttgt acaaatatac accgcttcaa
      961 acttcatttg gagtcaacaa tattgccttg gtcaaaggcc gtccaatgct gcttactctg
     1021 gcggatatga tgcgtcattt tattgagttc agaattgaag tgatcgttcg tagagctaag
     1081 tttgacctta ggaaagcgga agaacgtgcc catatcctgg aaggtttgct taaggcgctg
     1141 gatcatctgg atgaagtcat tgccctgata cgagcatcaa agacagtgga tgatgcgcat
     1201 tccgggttgg tggcacgatt tgaattttct gatattcaag caaaggctat cctggagatg
     1261 cgtctgcaaa gattaaccgg cttggaacgc cagaaaattc aggatgaata ccaggagtta
     1321 ttaaagacaa tcgcctacct taaggagttg ctccaaagcg aacagatgca gaaagacgtt
     1381 gtcaaagggg aattgatcga aataaaggag aaattcggag acgcaagacg gacagagatt
     1441 tcccatgctg aaggagatat atctatcgaa gatctcatag cagacgaggc ggttgcaatt
     1501 acgatttccc atctgggcta tattaagcgc accagtcttg atgaatataa gccgcagaaa
     1561 agaggtggcc gtggatctca aggaagtaaa acccgggatt cagactttat agaacatatg
     1621 tttgtcgctt ccaatcacaa ttatcttttg ttgtttactg agcagggacg atgcttctgg
     1681 ttaagggtat tcgaaattcc tgaagcatct aaagtttcta cgggacgcgt catccagaat
     1741 ataatcaacc tgcctcagga cgaccgcgtc aaggcatata tccccatcaa ggatttgaat
     1801 gacagggatt tcctggacaa tcattacatc cttttctgta ccagaaaggg gaccatcaag
     1861 aagactcctg taagcgattt ttctcgtcca agatccaacg gtattaatgc aatcaccatc
     1921 cacgacgggg atatgctgct cgaagcccga ttgaccaatg gagacaatga gatcatactc
     1981 gccaacaggc atggtagagc tattcgcttc aacgaaagta aagtacgttc aatgggacgt
     2041 acagctgccg gagttagggg aatgttactc gatggacctg acgatggaat cgtcggaatg
     2101 gtgtgtgtta atccgaatga tatgctggtt accatttttg tcgtttctga aaatggaaat
     2161 ggcaagcgtt ctgcattgga agactatagg gtgaccaacc ggggcggaaa aggcgtcaag
     2221 actatgagga tttctgaaaa gaccggtaat ctgattgcta ttaaggaggt gtatgaaatg
     2281 gatgagatga tgattacaac taagaatgga gtcatcatca gaatggctat ggaagccctg
     2341 cgggttatgg gtcgcgccac acaaggtgtc agggtgatca agctcgattc cggtgacaga
     2401 attgccgata tagctgtaat cagggaatca atgctttctg acaaaactga ggaagaatag
     2461 ttgcactgat tttaagaaaa aattaataga ttcgtggcag tatattgaaa cgatgacact
     2521 aatttgggcg ttaaaggaat gaattcaatg tattttttga acaataacaa tttaaaaatt
     2581 tgcctgagaa ggcttcagaa caaaaaacaa taatcaatga agaacttact ttggatacta
     2641 ttgatggtga caatcgtcaa catctcggct tctgcccagg atgatggtgc taagttggta
     2701 aaaaaggcaa aaaaggccat tacaaactat tttttggatc catccaacaa ggataatctt
     2761 gcagagtccc aacaactgat agaaaaagcc tttgccaccg ggacagttga taacgatgcg
     2821 gctgcctggg caactaaagg tgatatctat gccgaattgg ctaaagctga tgctgccatc
     2881 aaaatgatcg accccaatgt taaattaaag aatattgaag ccccttttat tgcttttgag
     2941 tcgtataaaa tgagtattgc aaaagcaaca aaggaaagtg caaagaaaga cgctaataac
     3001 ggtttgaatg aactgatacc aatgcttagc acatccggtc tggagtattt ttacgatcag
     3061 aactttgaaa aggcatttcg ggatttcaaa gcaattattg aggcttatga tatcgcaaag
     3121 agtgcaaaac ttaagaccgc actggacaag cctgaagatc tcaacaatca atattatgtg
     3181 acgggacttt cagctctcaa tgccggcaag gaagcagaag caggtgaatg gtatgagaaa
     3241 gcttttaatg gtggaaataa aggtgctgaa atctacgatg gattattcaa gtcctatcag
     3301 aaatctgatc ccgcaaaggc tgaaaaatat ctcacggaag gaagaactgc ctatccggat
     3361 gataacagtt tgctgttctc tgaaatcaat ctttatcttt ctcagggtaa acttagtagt
     3421 ctgattgata atttagagct agcttgccag aaggaacctg acaacatcag tgttataaat
     3481 acgcttggaa acgtgtatga caaactttat cagaatgcga gtgaaaaagg agatgctcag
     3541 gaggcagaga attatttcaa taaagcgatg gaaagttata ataaagcatt ggccaaagat
     3601 ccatcaaata actttgccaa ttacagtatg ggcacattgt attataacaa agccgctaac
     3661 tatgtcaagg taatgcagac gctaagtgat gatttgagta aggaaggtat gaagaagtat
     3721 gacgaggcac aaaagaatat gtttggcatg tttgatgcag cattgcctta cttccttaaa
     3781 gcggaggaat cagatctaaa gatagaaaca ccctgattgc cctaagagaa atctatgcca
     3841 gaaagaatca actggataaa gcagaagagt acaagaaaaa actggatgct ctttgatcag
     3901 gagccttgaa ggaaatggag aggggaagat gtatttcttc ccctctctgt ttttgtaaga
     3961 ttcattttgt tgtctgaaat tcttttaccc gtttctcatt tgaatgccca aattggttat
     4021 cacggtgtct caacattcaa tgacttttgt gggaaataat ccagcctggc ccatctgcag
     4081 tgcttgtttc aacacttttt ttgaggcctg gtaaccaaat gattttgtca tccaaaagaa
     4141 caaccggttg aattaatttt tgcaaataat ttaatcttgc attggtatag acatcctgaa
     4201 gtttttgact ctttccttcc atgcctaagg aacggattcg gtctccatcc tgacgagccc
     4261 tgatcttaat agttgactgt tttgcttttt ccggtatttt tatttcacct ttatccggta
     4321 ttcgattaga ctttgaaatc acgaagattg agtcatggta ttggtatttg ccttccacca
     4381 atggaaactc aagtgttatc gaagggactt cacgatccag gtagacaatt tttccccggt
     4441 cactgattac catactgtta tttgcaaaaa atcgtgcacc actttttcgt tctgaaagac
     4501 atctgctcat atcctcaatg tccgaatgag aaaaaccaag atcgcccatc cagaatatca
     4561 gcaagaagat tagtaagtca ggttccgaca gatcgttaag atccagaatc attccatatg
     4621 ggtttggttt gattctttga tgcaactctt tttctacccg tgtctccaca aatctaaaat
     4681 acaaatcttg aatcttcatg acatgagtca ggctgccctt cattcccggg tatcggtttt
     4741 ccaccagggg aatcacctga tttcttatga agttacggct atacttgtcg tccagattgc
     4801 tgctgtctga cctgaattgt aacttggcat ggtgacaaaa gactttcaac tcttcccttt
     4861 gaaagcccag caatggcctc aatatcttat tattccgggg aggtatactc cgtaagcctt
     4921 ttgggccggc tcccctcatc aggttgatga caaaggtctc aatccgatca tcctcatgat
     4981 gtccggacag aataaagtca aattgatttg attcacacaa ttggtagaac cagtcgtacc
     5041 tcaactttct ggcagtttcc tgtactgaaa ggtctaataa tcttgcttcc agtttcgtat
     5101 caaatgtcct ggtaaatacc ggaatctgat ggccggtagc aaactccaca acaaatgctt
     5161 cgtcctcatc actttccttg cctcttaact taaaattaca atgagctata gcgatatcaa
     5221 ggtccgacat tctacacaat tcgcttaagg caatggaatc cgaaccccca cttacggcga
     5281 ccaggtattt gaaccgggta tattcattgc cagcgagaac ttccagattt ttgttaaacc
     5341 gttgtaaaat attacgaaga aatgaattca tacagtccaa taaaagttat ctttatggcg
     5401 ttatcctgac aaaaatatta tcaaatattg aaaatactac ttttatttga atcaacccaa
     5461 ccaaatcttt atccggcaag taaaaatttg ccaaaaagat ttcaaccata gaagtgcccc
     5521 ttttttgatt gatcaactaa cttttataca tcatgattaa ttttactttg cgtttattga
     5581 ctttttgcct ttttttgaca ttcatccaat gtaagcctgg agataatggt acctcaagtg
     5641 tcgaagctac tccacgcact gattctgtga ggtttaagaa atatgttcct gaaccgggtg
     5701 atgtgccggt tgatccggag gcggatgaca atgccttcag accccagctc ccgttggatc
     5761 aggcagggtt taaatctgca aagcctgatg aagccagacg attagagaat gaatttgagt
     5821 ttatgtttga taaggaatgg aaaatcaatg gtcggattta tgccaatgac gataaagtac
     5881 acagaaaagg tcaggaagat tttaggttta acagagatgg tagctactcc ggtgtggtgg
     5941 aaggcaagga aatctccggt acatggatgg gtcgtttgga gaacaatgca cctgtcatta
     6001 ctgtatttcc cactgatttg aaagaaaaag taaacgaatg gtttgttaag aataccggta
     6061 agaccatggt ttggtccggt acgccgagtt ataaggacaa cggagtcatg attcaatttg
     6121 tattaaaaaa ataattaata ttcaaaaaac tgtttatttt tgcagtacac attttaaaat
     6181 aaaataatat gtttagtcgt aacctgcaaa gcttaactct tttcgtgttt atgatgggta
     6241 ttttttcaat tgcccattcc cagactgaca atactatttt caaagtagaa aaggctagtc
     6301 tcagtaggga tgtcctggag tacagcccat tatacctgaa aggcgactgg gtatatactc
     6361 agttggatga caagaatgtc aatatatatt cactggggag tgataaagtg atccctatcg
     6421 aagggttcag ccttagaagg gccattgtga ccggttttgc caagtcagat cagggtggat
     6481 tgattgccat ttcatatggc gacaataagc agaatgtacc ggcaaaatta aagggtaaca
     6541 aaatttattt tgcttttcag gacaagaacg gtattgcatc caaatatttt gagtttcaat
     6601 tgaatagtga agcgtattcc tgcactacac cctatcttag cccggacggg accaagcttt
     6661 attttgcttc tgatatgccg ggtggttatg gaggtttgga catatatgtt tgtgattacc
     6721 gtggaaaaga atggagtaaa ccaagaaatc tcggtccgaa gatcaattca gaaaaggatg
     6781 aggtgtatcc ttacatgatt gataatatgc tgtttttttc tagcaatggc cacggtgcca
     6841 ataggatgga tattttcatc gccgatatgg ataaggattt aggtcggctg gtaatcaatg
     6901 ccggaagccc aatcaacagt cctgccgatg attattcaat gagttttgat aacaataccc
     6961 gtataggtta cttcctttcc gatcgtgaag gagtgaatgg aaaagcgttc aaagtgacca
     7021 atgagaaaaa actgatcctc ctgaacatca agtccaatga ggataagaaa gcaatttccg
     7081 gggcaaaggt cgatttgtca agatgtcgta aaaatccact ttatgcgaat agtaagggta
     7141 gtgtaatcct gccggtagcc gcaggtgaga agtgttttat tcaggtaggt aaggttggat
     7201 acaattcagc tacaattcca atcgatttcg atgaaattat tggtttgaaa aaatccatca
     7261 atatcaacct ttcaaaggaa ggagtcttct atcaaggccg cgtggtggat caggataaca
     7321 atccggcaga tgaggtcgaa ttgtccattg tcgatcaggc aaccggtgaa ttacaatatg
     7381 tttttacgga tgacaacggc tactacacca tcgctttgga acccttgtcc tattatatga
     7441 ttcggtcaaa aagcccttat tataacccta gtgaaagcaa gattgttaca ttggcatcag
     7501 ttccggctga tattctggga accataaaac tggtgagcaa tggtagcagg cgatctggta
     7561 tcattaagac ttccagcatg gacgatatta aagttacgca gcgaaatgag tccgaaacag
     7621 agaatccttc gacttcagat tttgtcgaaa caggagtagc aaaaaaaatt gagagtgtca
     7681 agggacctga aacatccgtc actgacgctc aaaaagtaat ggattacaat gataacagat
     7741 tgagagaaga gacagtcaag gaagaagtta tgcaggagaa taagcagatt gcacctgccc
     7801 aatctccggt catagtcgca cctaagacgg aaaataagaa agaagctatt gtaaaatatg
     7861 ctatccagct tgctgctatt tctcttgaca atcataacct tcagccctat caggagaaaa
     7921 ccaggggtcg tggtcaggtc tatattgcaa gggaaggtaa tttaaataaa gttcgccttg
     7981 gtttctatga aacacgacag gatgcattgg ccatcagaga acaattgccc gaagaattac
     8041 gcaagggttt tattgtggag gtaaaagaaa gcgagtataa taataggcag gcgatacctg
     8101 ttgatcgtaa aaatgctatt gacccgccta tgaataagcc ggagaagaag gctgaacctg
     8161 atccaaggat acaacctgaa aacaagcctg aaatgaaatc ggaggtaaaa cctcaggaaa
     8221 agaccaattc tgagcctcag gttgtccaga agtccttgga agagtcaagg gtagaataca
     8281 aaataaggtt atcaaccttg agaaatgctt cccttttcaa tggttccgca gtcaggcagt
     8341 atggcttgat tgaagaaatt aaaaccggca atctgacaac tttttattta agtggattcg
     8401 aaaataaaga agaagtcaag caggttattg ataaggtaaa agcaggcggc ttcccgagtg
     8461 cacagattgt taagttaact aatggtgaat ataccattga caattgatac ctcttatatg
     8521 actcgttgaa cggaacagtc gttcctatag tttattattt ctaagggagg aggattatct
     8581 aattagctgg agaaattcat ttctggtctc aacattttta aattcaccgg taaaagccga
     8641 agtggttgtt acagagttct gtttctgcac gccacgcatc atcatgcaca tatgagaagc
     8701 ttcgattacg actgctacac catgcggttt cagtgtgttg tcgattactt cgagaatctc
     8761 ctgagtaagc ctttcttgta cctgaagtct tcgggcaaat acatccacta ctctgggtag
     8821 cttactcagg ccgacaatat aatcctttgg tatataggca atatgagcct ttccaaagaa
     8881 tggaagcata tgatgttcac atagtgaata tacttcaata tctttcacga tgatcatttc
     8941 attgtaaggt tcattgaaca tagctgatcg aaggatctca gctgcatcca ttctgtatcc
     9001 gctggtgagg aagtcaattg ccttagctgc tcttttgggc gttttgagaa gtccttcccg
     9061 ctccacatcc tcacctgaca aagtgatgat ttggttgtag tgattaatta aattatctat
     9121 acgggaatcc tgatattcat ttgtatccat ttaatatgcg ttttgatcga ttgtatatgt
     9181 ttaagggcaa atttaatctt tcatttaata accattcatt ataattaatt caggacgatc
     9241 cattgtgctt tttatttcag taattagatt gatcttgttc aaacaaccgg aaggcaatcg
     9301 gtgagaagta ttgatggtta ctgaaataca catttccaac tatactttat ctaaacatag
     9361 aaccgcctga aaagatgcaa cggcattaaa tggtgtgtga ttgattttca ataaaattca
     9421 attatattat gtaatctgga tttccggact tttctatcgc acgacaatgg aaataaaggc
     9481 ttaatcgccg ctctcatcct ttctgtcatc gttcacaagt tcttttgcta tgcaattgaa
     9541 ccgatcagat tgaatatagt ttcagaatac ttgcgaatgt ggatttttta ttgaatgcta
     9601 tgacatattg ctcatatatt gatatcttgc acaatgaatt tacgaaaaaa tttgaacttt
     9661 atacaagcgt gatggaaata cttgaagctg tttggcagta ttttcaaagc cataccctgg
     9721 aatttgttgc agcttttttt ggagtagtta gcgtatggta tagtaggaaa aatgatattc
     9781 ttgtatatcc tacaggaatc gtcagtgttt tactttacgt ctacatttgc cttatacata
     9841 aattatatgc cgacgccgga gtcaattttt attattttgt catgagcgtg gtaggttggg
     9901 tcaattggtc taaaaaaaag gaagaggagt ttgtttttcc tatttcatat tgcgatagga
     9961 atgagtggat aaaagggatt gctgtattcc tgataatcct gatttcgctc ctattctttt
    10021 tgttttattt tacggatagc actacagtag ttggtgattc tttggtttct gcatcagccg
    10081 ctactgccat gtggtggatg gctaagcgca agattgagag ctggtacgcc tggatcttga
    10141 gtaatatcgt agccttgcca ttattttttg ttaaggaact tcattttacg gttgtacaat
    10201 acattgtatt tctttatctt gctgtatggg gtttgattac ctggcggaaa gaatttaaaa
    10261 aagggtatgc aaaattgtga ttgtagtgta accgatatga tagatattgg tgatcagccg
    10321 tttaccagag tgcccccgac cagggccgga aatatgtttg ctgtggtggg ggtagaatct
    10381 acgggcaaat ccacatgcgc tgccttttta acagaggcgc ttaatggcaa attgatcgaa
    10441 gagtacgcca ggctctatct ggaagaacat tccaatacgt ataatcatga agattttctg
    10501 gagattgcca aaggccaggc gtacatggag aatcatgcca tggaaactgc ggctgaaggt
    10561 gatatattag tcttcgacac ggattatatt gtcatctacg tgtggtctat gattgttttt
    10621 aataacatcc cgcaatggat aacgaatcgt attgagcatt atccacctaa agtttatttt
    10681 ttgatgactc ccgaggtcga atgggtcaat gatgggttgc gggagtatcc tgatcttgag
    10741 gtcagggaaa gtatccaccg gatctatatc gaggttttgg aaaaattcaa ttgtaaatac
    10801 tttatcattt caggacctga ttatagggct cgccaggaaa aagcactgca gttggctctt
    10861 gaggaaataa ataaagcgaa ggggtaaggt gaagcaagga gattgaaaat gtatcaattg
    10921 agcttcaata tttttctggc aaaattccga atgaccctga atgaaaaaaa ggctgtcata
    10981 atgataagta tgccaaatac tatgggaatc aggaaagcaa aaatgagaaa gcagacacac
    11041 aatatatttt ccagagtact gtaagtcgaa ttggcaagtc cgtagaaaga ggccgttgtc
    11101 ttgatacgca tcacgccggt aaggctattg atactcaagg ctgtccctcc accgccaatc
    11161 aggctcaaca aatattcagc gtgaaatggc atctcaggca gaacggaaaa catactgagg
    11221 aatccggcac ataagaccaa tggcatgccg actatgtcca taaagtgatc aatgattgga
    11281 gtataaaaac ccaatatttc caatgatgtg gcaataccga gtgccaatgc tacatattta
    11341 ttgtccaacc atagaaattc gttgttaggt tgaattaaat cgaactgaaa tgtgagtgaa
    11401 atgaggaaga atggtaaaaa gaccctcagg ccggttgctg cacttaaccc gatgccgaac
    11461 aatatgggta tcagccagtc catattttat ttggagaagt tgacaatcgt ttctctgata
    11521 atcctgcaac aatccagcaa ctcgtcctca gtaatgacca atggaggagc aaaccggatg
    11581 atgttaccgt gggtaggctt ggctatcaag ccattctccg ccatttgaat acaaatatcc
    11641 cacgccgtgg aagaatcctc gctatcattg attacaatgg cattgaggag accctttccg
    11701 cgaactagtt tcaccaaatc acttgattct accagtttct gcatttcagc cctaaagatc
    11761 cttcctaaat attcggcctt ttgcgcaaga tcctcatcaa gtacgacctg catcgcttcc
    11821 attgccactg cacatgcgag tggatttcct ccaaatgtag agccatgttg tcccggtttg
    11881 atggttagca tgatttcatc gtcagccatc acacaagaga caggtaatac accaccggac
    11941 agagctttcc ctaagattag aatatccggg cggataccca gctgatcaca agccagcatc
    12001 ttgcccgttc gagcgatgcc cgtttgtacc tcatcagcaa taaataatat gtttttctcc
    12061 ttacagatgc tgacgacttc cttcaaataa gtgtcgtccg gaacacgcac acccgcctct
    12121 ccctggatag gctccatcaa aacacccgcc acctcatcat cttcaactgc ctttttgaat
    12181 gcttcgatat tattgaatgg tacttctaca aatccaggca aataaggagc ataaccgcct
    12241 ttactatcag gatcactgga aatacttatg gcagccatgg ttctaccatg aaagttacct
    12301 tcagcaacta tgatttttgc ggtccctgat ttttgaccgc gcacttcata ggcccatttt
    12361 ctgcacaatt tgatggcagt ttcgacagct tccacacctg tattcatagg taaaagttta
    12421 tcataaccga acaatccggt cataaatgct tcatactcac cgagcttgtc attgtaaaaa
    12481 gctctggaag tcagggtcag ccttgatgcc tgatttacca gggcctcaat tatccttgga
    12541 tggcaatgac cttgatttac agcagaatac gatgatagaa aatcgtaata tttcttacct
    12601 tctacatccc atacatatac accttcacct ttggtaagta ctaccggtaa tgggtggtaa
    12661 ttatgtgctc catgttgatc ttccagggtg atgaatgtag ttgcgctcct ttcttttgta
    12721 agcatgacag ttgttttagg ccacaaaggt aataataaaa agagaaaaaa gggttccgga
    12781 atcatttatc attgattccg aaaccctttt ttcagattcg ttttcacctc aggttatgat
    12841 cgaatagtag ttcctttaag gactacagtc ggttaactca acggatatca tgaatttata
    12901 taatcttcaa tcggcgggca ggtacatacc agattcctat ctccaaaggc attgttgacc
    12961 cggctgattc ctacccaaaa cttaaatcca tgacgtagat aatccaatgg gaatgctgcc
    13021 ttttccctgg aataaggaag gttccattca ttggctgtga ccaaaggcaa tgaatgtgga
    13081 gcattgacaa gcacattgtt gtccgctgca gccttaccgt ctgcgatttc ctccaattct
    13141 gccagaatat tcagcatggc atcacagaat ctatccaatt cgtccttact ctcactttcg
    13201 gtgggttcaa tcattaccgt tccggctacc ggaaatgaaa gggtaggagc gtggaagcca
    13261 taatccatta accgttttgc tacatcttct gcacttgcca gatgcttgta aggcctcagg
    13321 tcaacgatca tttcatgagc cgcacgacct tgagcgccgg aataaaggat aggaaatctt
    13381 ttttctaatc tcgccttgat ataatttgca tttaatatgg caatttcagt ggccttcttg
    13441 agaccacttc ctccaagcat tcgtacataa ccgtatgaaa tcagaatgat gctggcagag
    13501 ccgtatggtg cggagctcac tgcgaagtca tcgcccatct tggtgtgtcc cggcaggaaa
    13561 ggcttcagtt tatcgttgca acagattggc cccataccgg gtcctcctcc tccgtgaggg
    13621 atggcaaagg tcttgtggag attgaggtga catacgtcag caccgatttc tccgggattg
    13681 gttaatccca cctgtgcgtt catattggcc ccatccatat ataccatacc gccgttgtca
    13741 tgtataacct ggcaaatttc cttaacggaa acctcgaata ctccaaaggt cgatggataa
    13801 gtaatcatca gacatgacag gttatccctg tgttgtacag caagtgccct tagatcttca
    13861 agatcgacat tgccttcgtc atctgttttg actacaacca ccttcatgcc ggccatgacg
    13921 gctgatgcag gattagtacc gtgtgcggat gacggaatca aggaaatatt gcggtgatgg
    13981 tcgccattgg cttcatgata tcttcttatc gcaagcaaac ctgcgtattc tccttgtgca
    14041 ccactattcg gctgaagaga acatccagta aatccggtaa tttcgcacag gtattcggag
    14101 aaattcttga tcaactcata atatccggcc cattgatcac gtggtgcaaa aggatggata
    14161 ttggcaaatt caggccagga aacaggaatc atttcagaag ctgcattgag cttcatagta
    14221 caagatccca atgaaatcat actgtgcact aaggaaagat ctttattctc cagcattttc
    14281 aggtaacgca tcagctttga ctctgtctga taactgttga atacttcgtg agtcatgaaa
    14341 tccgaagttc ggacaagctc accggtaatt ggtgagttca aactttgcat atcggctttc
    14401 aattgctctg ttgttcggtg gatatcaaat gtattaagta tatcggctat atctgccaac
    14461 gaggtcgtct catccaatgc aatcaccacc acaccttcgt caagatatcc gaaattgata
    14521 ctcattgctt cggattttct cttcaatgct gcaatttctt cgctgctcat ggagagagct
    14581 atcgtgtcga aaaatacgtt gttgagcgtc ttcatcccag ctgtggtcag cgcattattt
    14641 aaggttacag ccagtgaatg catattcccg gctattcttt taatgcctcc tggtccgtga
    14701 tatacgccgt acatggcgga catgttagcg aggagtgcct gagcggtaca gatatttgag
    14761 gttgcttttt cacgcttgat atgttgttca cgtgtttgaa gtgccatcct taaagcacga
    14821 tttccatggg catcaatcga tacaccgatg atacgcccgg gaattgctct tttgaattct
    14881 tctccggtag agaaatacgc agcatgtgga ccaccgaaac ccatcggaac gccaaacctc
    14941 tgggaatttc ccaaaactac atctgctccc aactcaccgg gaggtgtcag caatgccagg
    15001 gccatcagat ctgctgcgac agctaccaga atacctcttt cctttaatgc tctgatatca
    15061 tcacctattg cgtgtacacc acccgtctta tccgggtatt gcatcagtac tccgaaagct
    15121 ttatccccaa ttctgtctgt tgatctgtca aattccacga cctcgatgtc aagcaccttg
    15181 catcttgtct tgacaacatc aagggtctgt gcatagacac ctttttcgac atagaattga
    15241 ttaaactcat cgccggactt gttcttgaat gcatagaaca tatgcattgc ttcagcagct
    15301 gctgtccctt catccagcaa agaggcattg gctattggca gtcctgtgag gtcagagatc
    15361 attgtctgaa aattgagtaa tgactcaagt cttccctggg atatttctgc ctgatagggc
    15421 gtatattggg tgtaccatcc cggattttca aacaaattcc gaagaattac attggggaca
    15481 atagtattgt aataaccttg accaatatag gatttgaaaa ccttgttcct agaagcaatt
    15541 cccttgacgt gttccagata ttcatgttcg ctcatcgctt ccggaaggtt aagctcgcgt
    15601 tgcaggcgaa tgctctccgg tacggtctga tcaacaagtt gtcccaaggt ttctactccg
    15661 atgacagaac acatttcagc gatctgttca ctacgtaatc ccaaatgtct gtcttcaaac
    15721 tttgactctt cgaaattcat gtat
//
LOCUS       DAOQMC010000022        15571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1368_length_15571_cov_5.267659, whole genome shotgun sequence.
ACCESSION   DAOQMC010000022 DAOQMC010000000
VERSION     DAOQMC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15571
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_15571_cov_5.267659"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(390..1082)
                     /locus_tag="PKV31_01740"
     CDS             complement(390..1082)
                     /locus_tag="PKV31_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91602.1"
                     /translation="MKPKRCLLFTCLIVGFLFFNACNKEDINTNTGSWSGQALNADTL
                     YFDNETQFRDFLFHYGLSRKYEIIPPKYHFSCTVVPLPNNYPEQMRDDYLNEDRFVGK
                     PFINLFDHIATRKYINIFFPTYYENNPGTTKVLNFCDVNEYITNLATGQVVCFKDVFN
                     WYDENGENIFNANKKVWLLWAYNDAVVKFGIEIVHVPTGVDYTFKEYYPAHNELDLTL
                     SDIAIKYGHLKP"
     gene            1252..2820
                     /locus_tag="PKV31_01745"
     CDS             1252..2820
                     /locus_tag="PKV31_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB91603.1"
                     /translation="MPVRIKNLAAHHHHPWFPITFVSMSIMKKLAGETIYYGISSIFG
                     RFINFFLTPIYTYSVIIDKAKMGHLTELMAYTAIILILFTCRMEAAYFRFGKDKDNQE
                     AAYRNSVTTVVSLSVFFGLLLFMLSPIIAPVLDYGGEHIYFQLLAVIMTIDAFCELPL
                     SRLRFEGKAKKFAFIRIINILTNVCFNIFFVVVCPRLYDAGYSFVTAWFDPEKIIVYI
                     FLSQVFGSLSSLILLSKYLKGVGRVDIPLLKRMVAYSWPLIIVSLVSMFNEMFGRVIL
                     KWFLTGSLSDNEAQLGVYGANYRLATIIALFTQAFRYAAEPFFFKESNNVGAEKTYAD
                     VTKYYLIFSIYGFLFVSLYIDIFKYFVGPKYFEGLKVVPILLLANVFNGLYFNVSIWY
                     RLKDKTLTGAMIAIFGAVLTLFFNWYLIPILGYIGSAIATLVCYSSMTIICFVLGQKV
                     MKVPYQVRTMLLWMGAAILIQIGIWYLRSLDISVYLVLLCSTIGIVLFSGLIWSYEKE
                     KIKTLLRSIKLRSR"
     gene            complement(2849..3979)
                     /gene="lpxB"
                     /locus_tag="PKV31_01750"
     CDS             complement(2849..3979)
                     /gene="lpxB"
                     /locus_tag="PKV31_01750"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNB91604.1"
                     /translation="MANKIFLLAGEASGDLHGANLATALKEARPNIVLEGWGGPKMEA
                     AGVALYRKLKDLAFMGFVEVLANIFTIKRNFSLIKNHLSKHKPDALVLIDYPGFNLKI
                     AKWAHRQGIRVFYFISPTVWAWKAGRANIIRESVEQLYCILPFEVEFYKQLNYDVTYV
                     GNPLVDSISRFTPDAGFLSENNLKKGDYIAILPGSRHQEVKRILPVMVGAMAQMPQKK
                     FVIARTSLLPEDEFTFILDQIHPDWKKNVSIVTDKTYDTIYHASSAMVTSGTATLETA
                     LLNTPQVVCYAANSLNIRIAKAFVKVKYISLVNLIPDMPIVQELIQEDLSVSNLTREI
                     LKLEDDADYRNKMLEAYGLLKTLVGEPGVANRMALDILHRIG"
     gene            complement(3979..4305)
                     /locus_tag="PKV31_01755"
     CDS             complement(3979..4305)
                     /locus_tag="PKV31_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91605.1"
                     /translation="MKANQPFFTKDSLILGVVIGTVVPIVLYALILMLKDALVSAGIV
                     PELFGEFRSSQRTIGIIALCGNLIVIHYFNNRRFGNAMRGLIFPTFAYVCWWVWEYGP
                     ELLDKF"
     gene            complement(4307..5077)
                     /gene="surE"
                     /locus_tag="PKV31_01760"
     CDS             complement(4307..5077)
                     /gene="surE"
                     /locus_tag="PKV31_01760"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNB91606.1"
                     /translation="MKKPLILVTNDDGMVAPGIAALVEVARDFGQVFVVAPDSPQSGQ
                     GHAITVENPLRLNQVDVFEDIVAYECNGTPADCVKLAKHLLHKNQHIDLCLSGINHGS
                     NASVNIIYSGTMSAAMEAAIEGIDSIGFSLLDYSFDADFTTAKQVVRTILEKVTRHGF
                     DADCRLLNVNIPKVAPDELKGIKVCRQADGYWMEEFVVGTDPRNQEYYWLTGKFTNRD
                     KGEDTDLYALQNGYASIVPSHYDLTHYKSIEKYKVLEE"
     gene            complement(5077..5988)
                     /locus_tag="PKV31_01765"
     CDS             complement(5077..5988)
                     /locus_tag="PKV31_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91607.1"
                     /translation="MRWKTLILIVPASRMLFVFCSLFMPSLCFGLHSDIKPSIGEPFS
                     TMRDTVYPGTDLAILKMDPLKKEGFPGEGIAINMIIMNYGFSESASTKIDFWVSEEDE
                     PDLDTNNINSTKLLAPLKMDTKVDFSHLVVIPADYTGRYVYITAVIRPNPKNGSDPTP
                     GNNTMFTAVRIGSADSYKGGIYSYDGAMKEITMVNPIRKLKRGKPYPFQVKFSITGVR
                     APKAGQYIDILLSADPVLDDTDATIASFPAIPGVNGVVKDIKKDLVFGKQFRKGKAYI
                     FAVIRPGKWKDPNMDNNYSKPLKIRLK"
     gene            6241..6585
                     /locus_tag="PKV31_01770"
     CDS             6241..6585
                     /locus_tag="PKV31_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNB91608.1"
                     /translation="MPYDNPSLTWDDFMKPDIRTGTILFAEAFPEARKPAYKITIDFG
                     EIGIKRTSAQVTGLYATDDLIGKQVVAVVNFPPKQIANFISECLLLGAVDGNYVTLLT
                     LDLPVRNGLRIG"
     gene            complement(6672..9515)
                     /locus_tag="PKV31_01775"
     CDS             complement(6672..9515)
                     /locus_tag="PKV31_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB91609.1"
                     /translation="MTNSFLSFGQNFELGTPPIRNFTKKIYAASPQNWQIGQRSDGMM
                     LFANNLGLLTFTGNSWDLYPIANRTIVRSLLVHPDQRIYIGGQGEFGFFDQKENGRLK
                     YKDLTPLIPQSTRNFADVWQLGYQDKYVLFQTTKEVLIYDGKSVRVYQPPATIKKMFS
                     YRRANYINLSDGSLLKFNGNSFEPVFIAENLPADIVNICDISNGNLRIAGYRSGIFDC
                     VGTKCSLNAFTLNPLYKNLRITVMQRLRDGHIMIGTMDHGLIIVSESGNVLLSLKKEN
                     GLINTSILAAMEDRNGNIWVSTESGIDFIEYKSPFRFLFPDIPYNGTGYSMALFRDRL
                     YLGTNNGLYYTEMLQDRSGPAIFREVAGSKDICWDLDVIDDNLWLGHNEGASIVADDH
                     LLPAFRGKGVWRFIKQDSQNIIFGSYEGFGMITQEGQQYVASILPGFDESSRIVLIDS
                     LFNIWMSHPYRGVYKLKKPHMGSPLSFRKYGLSGGLESDHENYIIKTNTRIYASNDKG
                     IFIYDPKRDQFRRDPTLESLIGYNTGTKLISADEYQNIWFRKGLKIGFLEPYKDEWKV
                     EYRQHILPALPESLAGGFEKVYPLTKDIFLFNCESGFLLFDKRKLIPDLQYTTYLYKC
                     TILSNRDSILLKGVEPNFIPYSKNKPLTLNHDQNSLRFDFSCFSFSDLQVEYKYTLTG
                     SSKSVSAWTSEPFVIYNNLDHGNYQISVQTRIGNVLQKGSIAFHFTIQPAWYNTLWFR
                     GSVLLCMIASIFGLFLLQDKRFKTEKKQLTQKHKRLVSEHADKVAQSEEEIIRLKNEQ
                     LQKDIQFKNNELASLAMHLAHKKDFIQHLESDLKTIQKEKTKNVDISANLARIIHRLQ
                     HESTLDEDWERFSHYFNEVNQGFILRLKEKYPNLTPNDHRLAAYLRMNLSTKDIAALS
                     NISIRGVEGSRYRLRKKMNLENDDNLHEIMNSI"
     gene            9838..11373
                     /locus_tag="PKV31_01780"
     CDS             9838..11373
                     /locus_tag="PKV31_01780"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91610.1"
                     /translation="MNRLLFLFFIMILVKSGIVAQDQIILDFETTETTTTFSYFDNGD
                     WPDAQTSVVVNPDKSGINTSDNVLYFVRGVGGQTWAGAYGNPKPTRKIDLTNHNRLKC
                     KVWAEKPGTVQLKLEGGPAGGADNWEKVLPIPATGQWVEMEWDVTLPGDAGEKVPAFG
                     KIYDNIVIFANFLQETTERVYLYFDDFATGEGEAAQPVPVTFVLNAAGYNGNISSASV
                     AGTFNGWDSGANVMAEVSAGSKLYTVTVDILPGVIEYKFVINGNTWEEGLNALDECVT
                     NNNGFINRKASISGETNLIPVCWNKCYNCGEYVSLTVNLGFGAANPPSPDGVWIAGGE
                     KFEAPGGKYRMEDSDGDGVYSINLTRGIGFKSHFTFANGNCADYSCKERLDDQACGDA
                     GNYWDRKLLPVTQDTVVYACFGDCSEVSCLASTKKVLDNPGFMIIPNPASSAVTLKFD
                     ENLRDRGSVLIRNIYGSVVKTVSYTPGMDVIKIEIESLTPGIYMASYVKDGMTGTKLF
                     IKQ"
     gene            11556..14555
                     /locus_tag="PKV31_01785"
     CDS             11556..14555
                     /locus_tag="PKV31_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB91611.1"
                     /translation="MNSQSLTGKVFENDSKETVVGATIRIVGYDKGTVSDEYGAYQLY
                     GLKTGDIIIFNSVGLKSRSVTYDGQTTLDIYLEREDKLLDEMVVVGYGSQKRSDISGS
                     VSVIKSDEITSSPALRVEQALQGRASGVLVTQNSGAPGAPLTVRIRGTGTINNSDPLF
                     IVDGVPVGGLDFLNPSDIESVNILKDAASAAIYGARGANGVILITTRSGKHNQEGVIN
                     YSSYYGIQNAWRKPRLLNAREYAIISNEAHINAGVKPLPEFADPDALGVGTDWVSAVM
                     GSAPITEHQLGMTGGTEKSTYAVSGNYFSQAGIVGRQKSDFDRITGRVNINHRVKPWL
                     TIGNTINYTWLSRTALTDNSEFNSPLIRALNIDPVTSIRKPDGTFNYSPYTDTDIANP
                     VNAIEQTYNRWITNRVVGSVFAEVEIIKGLKVRSAYSVDNTFANSRNFYPKYDLSIDP
                     VLSDAPATEKNLTNSVSFNDNRWTNWQWENTINYKYNLNEAHHFDMLAGTSALLNKHY
                     YNGGANTNLPSNKWEDAYISNTIDPITSQSAYEGASESALNSYFGRINYDFEGTYYAM
                     ATLRADGSSRFGVNNRWGYFPSFSAGWTFTNDGILDKVLHFGKLRASWGQNGNDNIGN
                     YGYTSVISSGQNYVFGPGQVITNGSVALVAPNPDLKWETSTQTNIGADLDFFNGRLQV
                     ITDYFIKKTSDMLYAIPVLSTSGTGAPVQNVASALNRGWELAVNFRSPYKSPVQYSLS
                     GNATFIHNRVTNLGTGGEPINSGRIQSANSTVARTDVGHPLASFYGWVTDGIFQTEEE
                     VKNHAFQSEGTSPGDIRFRDINEDGVIDIKDRTYIGNPIPKMSYGLTGNIAYKGFDLN
                     MFWLGSYGNDIYNATVRYDFTYVNRPESTLNRWTGPGTSNTEPKVSLNDPNQNARVSD
                     RFVEDGSFLRLKNIQLGYSIPTRWMSKVRVRELRIYAAAQNLFTFTRYSGMDPEIGIV
                     GNALELGIDKGFYPQARVFMGGIELKF"
     gene            14568..>15571
                     /locus_tag="PKV31_01790"
     CDS             14568..>15571
                     /locus_tag="PKV31_01790"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNB91612.1"
                     /translation="MMKRQNSLIFVLGLLAVFSISCEKEFLDRKPIIGVTSNNFYKTE
                     ADALAAVNAAYATLQFELSPAGHFRWFWGDIMSDDSQKGGSGDNDVVDLMKLETFQGP
                     TNTDLLESEWNADYEGIYRANVVLEKVPGIEMDEVLKKRILAEAKFIRAWFYYNLNTI
                     FGGVPLADKVLAPSEYNRPKEDAAVIWAFIEKDLKEAIEDLPQRSQYQAKDLGRITKG
                     AARALLAKAYLYQGKYAEAGFQTGEIIQSGEYSLVADYGTIFTKAGENNAESVFEIQY
                     MNASGGNWGKNAANEGTFTNVFQRARGQFAGYGFNIPTQDFVDEFFKEGFEDPRLKYT
                     V"
BASE COUNT         4365 a         3238 c         3598 g         4370 t
ORIGIN      
        1 taacatccag ataaatatct tccacttcat atccaaacac tatggccgcc tcgatttcct
       61 tcaagccttc aacgacaaaa acaccttgct tccgccggaa ggaattgtca gaaaccaggc
      121 gtaacagttt tttaaatttg tcattatgtg ggctctccag caaaagcatc tccaataaat
      181 taagatgcaa ggtaaagaag ttcagacaaa ttgttgagta gcatatgcgg aaataaagtt
      241 atcacaaaca ggaggaagcc ggaaatagtt acataatagt aagtgtatta aattatttca
      301 ggtgaagctg aaaacggagc aggtctgtat tttaaagcat gatgacatat tttaaaaatc
      361 attaccaaca aaaaagttgc ttgttggaat caaggcttga gatgtccgta ctttatggca
      421 atatcagaca aggtaaggtc cagttcattg tgagcgggat aatattcctt aaaagtatag
      481 tctactccgg taggcacatg cactatttcg atgccaaatt tcactacagc atcgttgtat
      541 gcccaaagaa gccacacctt cttgtttgca ttgaagatat tttcaccatt ttcatcatac
      601 cagttaaaga catctttaaa acagacaacc tgacctgttg caagattagt gatatactca
      661 ttaacatcac aaaaattaag gaccttggtt gtgccgggat tgttttcgta ataagtcgga
      721 aaaaaaatat tgatgtattt ccgggtggca atatgatcga ataggttgat aaagggcttg
      781 ccgacaaatc tatcctcatt gagataatcg tcacgcatct gctcaggata attattggga
      841 agagggacaa cagtacacga aaaatgatat tttgggggaa tgatctcata tttccgggaa
      901 agaccataat gaaaaagaaa gtccctgaat tgagtttcgt tgtcaaaata cagcgtgtct
      961 gcattcaggg cctgacctga ccagctaccg gtattcgtat taatatcttc cttattacag
     1021 gcgttaaaaa acagaaatcc gacaatcaga caggtaaaaa ggaggcatcg tttcggtttc
     1081 atatcgttgc ttttgtagtt tggatggcca ttttgctaaa gagtttaatt cactttgttg
     1141 aaaaaataaa ttattaatcg ttgaaaggat tgctatctca agataggttg actactcaac
     1201 gtattaggca atagaaaggc tttcagtctt tttgggggag ggtgttttaa tatgcctgta
     1261 cgtattaaaa acttagccgc acaccaccat catccatggt ttcctattac ctttgtgtcc
     1321 atgtccatca tgaaaaaact ggcaggagag accatttact atggtatcag cagcatcttt
     1381 ggccgcttta tcaatttttt cctgacgccc atctatactt actccgttat cattgacaaa
     1441 gctaaaatgg gacatctgac ggaattgatg gcctataccg cgataatcct cattctcttc
     1501 acatgcagga tggaggcggc ctattttcga ttcgggaagg acaaagacaa tcaggaggcg
     1561 gcctatcgca actcggtaac cacggtggtt tcattgtccg ttttctttgg attattgcta
     1621 ttcatgctgt ctccgatcat tgccccggtc ctggattacg gcggagagca catttatttt
     1681 cagctgcttg ccgtcatcat gacaattgac gccttctgtg aattaccgct ttcccgtttg
     1741 cggttcgaag ggaaggccaa aaaattcgca tttattcgca tcattaatat cctgaccaat
     1801 gtatgcttca atattttctt tgtcgttgta tgtccgcggt tgtatgatgc gggatattcc
     1861 tttgttacag cttggtttga ccctgaaaag atcattgttt atattttcct gagtcaggtc
     1921 tttggctctc tttcttcatt gattttactg agtaaatatc taaaaggtgt aggccgggtc
     1981 gacattccat tgttgaagag gatggttgct tattcctggc cattaattat cgtcagcctg
     2041 gtatctatgt tcaatgaaat gtttggtcgt gttatcctga agtggttcct taccgggtct
     2101 ttatcggaca atgaagccca gctgggcgta tatggcgcca attatcggct ggcgacgatc
     2161 atagccttat ttacccaggc attcagatat gctgccgagc cattcttttt taaggaatcc
     2221 aataatgtgg gcgctgaaaa aacctatgcc gatgtgacaa aatactatct gatcttcagt
     2281 atctatggct ttctctttgt tagtctgtat attgatatct tcaaatattt tgtcggcccc
     2341 aaatattttg agggattaaa agtggtgccg attctgcttc ttgccaatgt attcaatggc
     2401 ctttacttca atgtgtccat ttggtacagg cttaaggaca agacattgac cggtgcgatg
     2461 attgcgattt tcggagctgt tttgacgctt ttcttcaact ggtatctgat tcctattctc
     2521 ggttatatcg gttctgctat tgccacactg gtctgttatt cttccatgac catcatttgc
     2581 tttgtactgg gccagaaggt gatgaaggtt ccttatcagg tcaggaccat gctcctttgg
     2641 atgggggcag ccatattgat acagattggc atttggtatt tgagaagttt ggatatttcg
     2701 gtataccttg tgttgttatg ttcaaccatc ggtattgtat tgttttccgg cctgatctgg
     2761 agttatgaaa aggaaaaaat caagaccctg cttcggtcta tcaagcttag gagccgatag
     2821 taagaagtaa tttgtggata aatgagacct atcctatccg gtgcaggata tccagtgcca
     2881 tcctgtttgc cacaccgggt tcgccgacga gtgtctttaa taatccgtaa gcttccagca
     2941 ttttgttgcg ataatcggcg tcgtcttcca gtttcagtat ttcccttgtg agatttgaca
     3001 cggaaagatc ttcctggata agttcctgta ctataggcat atcgggaatc aagttgacca
     3061 atgaaatata tttgaccttg acaaatgcct ttgctatgcg aatattcaga ctgttggcgg
     3121 cgtagcagac tacctggggc gtgttgagca aggcagtttc cagcgtcgcc gtaccgctgg
     3181 taaccatagc cgaggatgca tggtaaatgg tatcgtaggt tttatcggtg acgatcgata
     3241 catttttctt ccaatcagga tgaatttggt ccaggatgaa agtgaattca tcttcaggca
     3301 ataaggaagt cctggctatt acaaattttt tttgaggcat ctgagccatg gccccgacca
     3361 ttacgggcag gattcttttc acttcctggt gtctactgcc cggcaatatg gcaatataat
     3421 cccctttttt taagttgttt tcagagagaa atcctgcgtc aggtgtgaaa cgcgagattg
     3481 aatcgaccag agggttgcct acataggtaa catcgtaatt caactgtttg tagaattcga
     3541 cttcaaaagg aaggatacaa tacaattgct caacggactc gcggatgatg ttggcgcgac
     3601 ctgccttcca ggcccataca gttggagaaa taaaataaaa aacacggatt ccttgccggt
     3661 gagcccactt cgcgatcttc agattgaatc cgggataatc gattaacacc aatgcgtccg
     3721 gcttgtgctt ggaaaggtgg tttttgatca ggctgaaatt cctttttatc gtaaagatat
     3781 tggccaacac ttcaacaaaa cccatgaagg caagatcctt gagctttctg tacagggcga
     3841 ctcctgcggc ttccatcttc ggaccacccc agccttccag cacaatattg ggtcgtgctt
     3901 ctttgagggc agttgctaaa ttcgccccgt gaaggtcccc ggatgcctct ccggccaaaa
     3961 gaaatatttt atttgccatc aaaacttatc aagcagttcg ggtccatact cccaaaccca
     4021 ccagcataca taggcaaagg ttgggaatat caatccgcgc atggcattgc caaagcgacg
     4081 attgttgaaa taatgaataa caatcagatt gccacaaagc gctattatcc cgatggtccg
     4141 ctgggaagag cggaactcgc cgaacagttc gggcacgatt cccgcagaga ccagggcatc
     4201 cttaagcatc aatatgaggg catacagcac aatggggacc acggtcccta tgacaacgcc
     4261 cagaatcaaa gagtctttag taaaaaaggg ttggttggct ttcatattat tcttccagga
     4321 ctttgtattt ttcgatagac ttataatgag tcaggtcgta atgggatggc acaatggaag
     4381 catatccgtt ttgcagggcg taaaggtcag tatcttcacc tttgtcacgg ttggtaaact
     4441 ttcccgtcag ccaataatat tcctggttgc gcggatcggt accgacgaca aattcttcca
     4501 tccaatagcc atcggcctga cgacatacct ttatcccttt aagttcatcg ggagccacct
     4561 tgggaatatt gacattaagc aggcggcagt ctgcatcgaa gccatgtctg gtaacctttt
     4621 caagaatggt tcgcacgact tgcttggctg tggtgaagtc tgcatcgaaa ctgtaatcca
     4681 acagtgaaaa tccaatgctg tcgattcctt cgatagccgc ctccatggct gcactcatag
     4741 ttccggagta aataatattg acggatgcgt tggagccgtg gttgatgccg gaaaggcaaa
     4801 ggtcgatgtg ctgatttttg tgaagaagat gtttggcaag cttcacacag tcggccggtg
     4861 tgccattaca ttcataagcg acaatatctt caaacacatc cacctgattg agccttaggg
     4921 ggttttcgac agtgatggcg tgtccctggc ctgattgtgg actgtcggga gccacgacaa
     4981 atacctggcc gaaatcacga gccacctcga cgagggcagc gataccggga gcgaccatgc
     5041 catcgtcatt ggtaactaat atcaatggtt ttttcattat tttaacctga tttttagcgg
     5101 tttggaataa ttattgtcca tattggggtc tttccatttc cccggacgaa tgactgcaaa
     5161 gatataagct ttcccttttc tgaactgttt gccgaagact aaatccttct taatgtcctt
     5221 tactactccg tttacaccgg gaattgcagg gaatgatgct attgtggcat ccgtatcatc
     5281 caggaccgga tcggctgaaa ggaggatgtc tatatactga ccggcttttg gtgccctcac
     5341 acccgttata gaaaatttga cctgaaaagg atagggttta ccacgtttta atttcctgat
     5401 gggattaacc atggtgattt ccttcatggc accgtcatag ctgtagatgc cgcccttgta
     5461 actatcggca cttccaatcc tgacagccgt aaacatcgtg ttgttgccgg gagtgggatc
     5521 ggaaccattc ttgggattgg gcctgattac cgcggtaata tatacatatc tccccgtata
     5581 atcagccggg atgacaacca gatgagaaaa atcgaccttg gtatccatct tgagcggagc
     5641 aagcaattta gtcgaattga tattgttggt atccagatcc ggttcatctt cttcgctaac
     5701 ccagaaatca atctttgtag atgccgattc tgagaagcca taattcatga taatcatgtt
     5761 gatggctatg ccttcacccg ggaaaccctc tttctttaaa gggtccattt tcaggatagc
     5821 aaggtcagta cccggatata ccgtgtccct catggtagaa aaaggctcgc cgatggaggg
     5881 tttgatatcg gaatgcaggc caaagcacag ggaaggcata aacagagaac aaaagacaaa
     5941 tagcatcctg ctcgccggaa caatcagaat gagcgttttc caacgcataa acccaacaaa
     6001 tgtgggtgcc gcggcgcctc tatattgagg aggaatgcct ttccacagat aaataatgaa
     6061 atggttaaat aaatcgaatg tcatcaatat tcccgaatca atttgtaata ttaataaaat
     6121 acaaggattt attctcaata aatgacaagt tgaataagga tttgttttaa taatgcatca
     6181 aattcgagcg gaagaattgt attatttttg gaagaatatt gtccattaaa caacctaacc
     6241 atgccctatg acaatccttc cctcacatgg gatgatttca tgaagccgga tatccgcacc
     6301 ggaaccattt tatttgcaga agcgtttccg gaggcgagaa aaccggcata taagattacc
     6361 atagacttcg gggaaatagg catcaagagg acttcggcac aagtgaccgg cttgtatgcc
     6421 accgatgacc tgatcggaaa gcaagtcgtc gcggtagtca attttccgcc caaacaaatt
     6481 gccaatttca tcagtgaatg tctgttactg ggggcagtag acggcaatta tgttacactg
     6541 cttactcttg atctgcccgt gagaaatggc ttgagaatag gatgacaaaa gaatttagcg
     6601 gcaaagggat attacaaccc taaataaaac atcttgttga accatatctg tggagaaaat
     6661 agctcgttgt tctatattga attcattatt tcatgcaggt tgtcgtcatt ctccagattc
     6721 atcttcttcc taaggcgata ccgacttcct tccacaccac ggatcgatat gttggagaga
     6781 gcggcaatat ccttagtgga aaggttcata cgaaggtatg ccgctaatct gtgatcattg
     6841 ggtgtcagat tgggatattt ttcttttagc ctgagaatga aaccttgatt gacctcattg
     6901 aaataatgtg aaaaacgctc ccaatcttca tccagagtgc tttcgtgctg gagtctgtgg
     6961 atgatcctcg ccagattggc agaaatgtcc acattttttg ttttttcttt ttggatggtt
     7021 ttcagatcgg attcaagatg ttggataaaa tcctttttat gtgccaggtg catggcaaga
     7081 gatgccaact cgttgttttt gaattgaata tctttttgca gttgttcgtt tttgagacgg
     7141 ataatctctt cttcgctttg tgcgacctta tcggcgtgtt cactgacaag ccttttatgc
     7201 ttttgggtaa gttgtttttt ttccgtttta aatcttttgt cctgcaacag gaatagccca
     7261 aaaatggagg cgatcataca taaaagcacg ctaccgcgga accacagcgt attgtaccat
     7321 gccggttgaa tggtgaaatg gaatgctata ctgccttttt gaagtacatt tcctatgcga
     7381 gtctgaacgg agatttgata gttgccgtga tccagattat tgtagatgac aaagggttct
     7441 gatgtccagg ccgaaacgct cttactactt cccgtgagcg tatatttgta ttccacttgt
     7501 aggtcgctga agctgaagca gctgaagtca aacctcaggc tattctgatc atggttcaga
     7561 gtcagtggtt tgtttttcga ataaggaatg aaatttggtt cgacaccttt gagcagaatt
     7621 gaatcccggt tgctcagaat ggtacattta tacaggtatg ttgtgtattg gaggtcggga
     7681 atcagttttc ttttatcgaa taaaaggaag cccgactcac aattgaagag gaagatatcc
     7741 ttggtcaaag gatatacctt ttcgaagccg cctgccagac tttcgggcag tgcaggaagg
     7801 atatgctgcc ggtattcgac tttccactca tccttatagg gctccaggaa gcctattttc
     7861 agaccctttc taaaccaaat attttgatat tcatcagcgg agatgagctt tgttcccgta
     7921 ttgtatccga taaggctttc gagggtcggg tcgcgcctga attgatccct cttcgggtcg
     7981 tagataaaaa ttcctttgtc gttggatgca tatatacggg tattcgtttt aatgatataa
     8041 ttttcatggt cggactcaag gcccccggac aaaccgtatt tacggaagga aaggggggat
     8101 cccatatgtg gtttcttcag tttgtacact ccccgatagg gatgagacat ccagatattg
     8161 aacaaggaat ctataagcac aatcctgctt gactcatcaa atcccggcaa tatggatgct
     8221 acatattgtt gaccttcctg agtgatcatt ccaaatcctt cataggatcc aaagattata
     8281 ttttgactat cttgtttaat gaatcgccac acacctttgc cccgaaaagc gggcagtaaa
     8341 tggtcgtcag ctacaatgga agccccttcg ttatgcccca accacaggtt gtcgtcaatg
     8401 acatcaagat cccaacaaat atccttgctg ccggcgacct ctctgaatat ggcagggcca
     8461 gacctgtcct gcagcatctc tgtgtaatac agcccgttgt tggtgccaag gtataatctg
     8521 tcacgaaaca gagccattga gtatccggtg ccattatagg ggatatcagg aaatagaaat
     8581 cggaaagggg atttgtattc gataaagtcg attccggatt ccgtagatac ccaaatattg
     8641 ccgttcctgt cttccatagc agccagaatg gaagtattga ttaagccatt ttcctttttg
     8701 agagacaaaa ggacatttcc ggattcactc acaataatca gcccatggtc catggttcct
     8761 atcatgatat gcccatcccg aagacgttgc atcacagtaa tccgcaaatt tttatataag
     8821 ggattcaagg tgaatgcatt caggctacat tttgtaccga cacagtcaaa aataccggag
     8881 cggtaacctg caatcctcag atttccattg cttatatcgc atatgtttac gatatcagca
     8941 gggagatttt ctgcgataaa taccggttca aaactattac cattgaactt aagcagggag
     9001 ccatcagaaa ggttgatata attagcccgg cgataggaaa acattttttt aattgtagca
     9061 gggggctgat atacacggac acttttacca tcataaatca gaacctcctt agttgtctga
     9121 aagagaacat atttatcctg ataaccaagt tgccaaacat ctgcaaaatt tcgggtagat
     9181 tgcggaatca acggggttaa gtccttgtat ttcagccggc cattctcttt ttggtcaaaa
     9241 aagccgaatt ctccctgccc acctatgtat attctttggt cagggtggac caatagtgat
     9301 cggacgatgg tccggttggc gatgggatac aggtcccagg aattgccggt gaaggtaaga
     9361 agcccaagat tattggcaaa cagcatcatg ccgtcactac gctgccctat ctgccagttt
     9421 tggggagagg cggcatagat tttcttggta aaatttctga ttggcggtgt tccaagctca
     9481 aaattttggc caaaggagag aaaagaatta gtcatcagaa aacaaaataa aagaatacgg
     9541 gtgggtaggt agcggagagg gtaaaaaaag tacttgtcgt agtaaaaatg cattcccgta
     9601 tttcaaaaat aagcagtaaa agtaaaaaat aaacaacaat gtcgtagtaa agaagtagtg
     9661 aataattttg aattcttaag catgttcggt atatttgttg cgatataagg cggtgcccgg
     9721 tattttttat ttggcagtgc ggccgtatcg aacaggggag attatggggt gattttttca
     9781 tatgtcgtat agccttccat cattttaatc atttatcaac ttaaaaaatt tagtattatg
     9841 aatcgacttt tattcctttt cttcatcatg atcttagtaa agtcagggat cgtggctcaa
     9901 gaccaaataa ttctggattt tgaaacgact gagaccacaa cgacgttcag ttattttgat
     9961 aatggcgact ggccggatgc acagacttca gtggttgtta atcccgacaa gtccggaata
    10021 aacaccagtg acaatgtact ttattttgtc cgtggtgttg gtggtcaaac atgggccggt
    10081 gcatatggaa atcctaaacc taccagaaaa attgatctga ccaaccataa taggcttaag
    10141 tgtaaagtat gggcagaaaa accgggtacc gttcagctta agttggaggg tggccctgca
    10201 ggaggtgccg acaactggga aaaagttttg ccaattccgg caaccggcca atgggttgag
    10261 atggaatggg atgtcacatt accgggcgat gcaggcgaaa aggttccggc atttggtaag
    10321 atatacgaca acattgtaat ctttgcaaat ttccttcagg agactaccga aagggtctat
    10381 ttgtattttg atgactttgc taccggagaa ggcgaagcgg cacaaccggt acccgttacc
    10441 ttcgttttaa atgcagccgg atacaatggc aatatttcct cggccagtgt agcgggaacc
    10501 ttcaatggtt gggattccgg agccaatgta atggctgaag tttctgccgg atccaagtta
    10561 tatacagtta cggtggacat attgccaggt gtcatagaat ataaatttgt catcaacgga
    10621 aatacctggg aagaagggtt gaatgcgctg gatgagtgcg tcaccaacaa caatggattt
    10681 atcaaccgga aggcctccat tagtggggaa accaatctga ttcctgtatg ctggaacaag
    10741 tgttataact gtggagaata tgtaagcctg acggtcaatc ttggcttcgg agcggccaat
    10801 ccaccatcgc cggatggcgt ttggattgcc ggtggcgaaa aatttgaggc tccgggaggc
    10861 aaatatcgaa tggaggactc tgacggagat ggtgtttact ctatcaatct gacaagaggc
    10921 attggattca agtcccattt tacttttgcc aatggtaact gtgcagacta ttcctgcaag
    10981 gaaagactgg atgaccaggc atgtggagac gcaggtaact attgggacag aaagttgttg
    11041 ccggtaactc aggatactgt tgtatatgca tgcttcgggg actgttctga agtctcttgt
    11101 cttgcttcaa caaagaaggt cttggacaat ccgggcttta tgatcattcc caacccggcc
    11161 tcaagtgctg ttaccctaaa atttgacgag aacttacgcg ataggggttc tgtattaatc
    11221 cgtaatattt atggaagtgt agtaaaaaca gtttcttata ctccgggaat ggacgttatt
    11281 aaaattgaaa ttgaaagttt gactccggga atatatatgg cctcatatgt taaggatggt
    11341 atgacgggta ctaagttatt tattaagcaa taattgatta aatgttgccc ggcattatga
    11401 atatgccggg catttttttc atggatactt tggattttta ggggattcca ttcggcaccc
    11461 ccgggttaaa tttttacaaa aatcagatgt ttttttccaa taaaatcttg atatgcactg
    11521 ccacagtgtt gctgtgcttg attttccctt cgttgatgaa ttcacaatca ttaacgggga
    11581 aagtatttga aaatgattca aaagaaaccg tagttggtgc taccatccgg atagtcggtt
    11641 acgacaaggg tacggtatcg gatgaatatg gtgcctacca gctatacgga ctcaaaaccg
    11701 gagacataat catcttcaac tcggtaggat tgaagtcccg atcagttact tatgatgggc
    11761 aaaccacgct cgatatttat ctggaaagag aggacaagtt gctcgacgaa atggttgtcg
    11821 taggatacgg atctcaaaaa cgaagcgata taagtggatc cgtatctgta ataaaatcag
    11881 acgaaatcac atcctcaccg gctcttcgtg ttgaacaagc tttgcagggg agggcgtccg
    11941 gcgttctggt gactcaaaat tccggtgcgc ccggtgcacc gctaactgta agaataagag
    12001 gtacaggcac gatcaacaac agtgatcctt tgtttattgt cgatggcgta ccggttggtg
    12061 gtcttgactt cctgaacccg tctgatatcg aatcagtcaa tatccttaaa gatgctgcat
    12121 ccgccgcaat atatggagcg agaggagcca atggtgtgat tctcatcacc acacgctccg
    12181 gcaaacacaa tcaggaggga gtaatcaact attcatcata ttatggtatc caaaacgcct
    12241 ggagaaaacc ccgacttctc aatgcccgcg aatatgcaat catatccaat gaggcacata
    12301 tcaatgcagg ggtcaagccc ttgccggagt ttgctgatcc tgatgccttg ggtgtcggta
    12361 cagactgggt tagtgcagtt atgggttcgg cgccgataac cgaacaccaa cttggaatga
    12421 ccgggggtac agaaaaatca acctacgcag tttctggaaa ctattttagt caggcaggaa
    12481 tagtgggcag acaaaagtcc gactttgacc gcatcacagg ccgggttaat ataaaccatc
    12541 gtgtcaagcc ttggctcacc attggcaaca caataaacta tacgtggctg agcaggacag
    12601 cactcaccga caatagtgaa ttcaattcgc cccttatccg ggcactcaat atcgacccgg
    12661 taacctcgat caggaagcct gacggcacct tcaactattc gccctatacg gatacagata
    12721 ttgccaatcc ggtcaacgcg atagaacaga cctacaaccg ctggataacc aatcgtgtgg
    12781 tgggcagtgt ttttgcagaa gtagaaatta tcaaaggcct gaaagtaagg tcggcctaca
    12841 gtgtggacaa cacctttgca aactccagga acttttatcc caagtatgat ttgagtatcg
    12901 accctgtgtt gagcgatgcg ccggcaacag aaaaaaatct caccaactcg gtttccttca
    12961 atgacaacag atggaccaac tggcaatggg aaaacacaat caattataag tacaacctga
    13021 atgaggcaca tcattttgat atgctggcag gcacatctgc cttgctgaat aagcattatt
    13081 ataatggtgg agcaaacacc aacctgccgt ccaacaaatg ggaagatgcc tatatcagta
    13141 atacgatcga ccccatcaca tcacaatcgg cttatgaagg agcgtccgaa tcagcactca
    13201 attcgtactt tggcagaatc aactatgatt ttgagggtac ttattatgcc atggctaccc
    13261 taagggctga tggaagctcc cggtttggtg tcaataaccg ttggggatac ttcccttcat
    13321 tttccgcagg atggaccttt accaatgacg gcattttgga caaagtacta cattttggaa
    13381 aattaagagc aagctggggt caaaacggca acgataatat cggcaactat ggctatacct
    13441 cggtaataag ctcaggtcag aattatgtct tcggcccggg acaagtgatt accaatggaa
    13501 gcgtagccct ggtagccccc aatcctgatc taaagtggga gacaagcaca cagactaata
    13561 tcggtgcaga tcttgacttc ttcaatggtc gcctgcaagt aattactgac tattttatca
    13621 agaaaacatc ggacatgctg tatgctattc ctgtgcttag tacatccggc accggagctc
    13681 ccgtacaaaa cgttgcatca gcactaaatc ggggatggga gcttgccgtt aactttagat
    13741 ccccttacaa aagccccgtt caatacagtt taagcgggaa tgccactttt atacacaata
    13801 gggtaacaaa cctcggtaca ggaggtgaac ctattaacag tggccgaata cagtcagcca
    13861 attctaccgt tgcacgtacg gatgtgggtc atccactggc atccttctat ggctgggtca
    13921 ccgatggcat cttccagaca gaagaagagg tgaagaatca tgcgtttcag tcggaaggta
    13981 cgtcaccggg agatatcaga ttcagggaca tcaatgaaga tggggtgata gatatcaagg
    14041 acagaactta tataggcaat cctataccga aaatgtccta tgggctaaca ggaaacatcg
    14101 cgtacaaggg atttgatctg aatatgttct ggctgggcag ttacggtaat gacatttaca
    14161 atgccacagt aagatatgat tttacttatg tcaacagacc cgaaagcacg ttgaaccgct
    14221 ggacaggacc ggggacatct aacaccgaac ctaaagtcag tttgaatgac cctaatcaga
    14281 atgcccgtgt atcggatcgt tttgtagaag acggctcctt cctgcggctg aagaatattc
    14341 aactgggcta ttccatccct actcgttgga tgtccaaggt cagggtgcgg gaattacgga
    14401 tctatgctgc tgcgcagaac ctgttcacct ttacgcgcta ttccgggatg gatcctgaaa
    14461 tagggattgt aggaaatgcc ctggaattgg gcattgataa aggcttttat ccccaggcga
    14521 gagtttttat ggggggcatt gagttgaagt tttaaagaaa aatgaaaatg atgaaaagac
    14581 aaaatagctt gatttttgta ttagggctgt tggccgtgtt cagcatttct tgtgaaaagg
    14641 agttccttga tcgaaagccc atcatcggtg tcacatccaa caacttttac aaaacagagg
    14701 cggacgcttt ggcagcagta aatgccgcct atgccacact acagtttgaa ttaagtccgg
    14761 caggacattt ccgctggttt tggggcgata ttatgtcgga tgattctcag aaaggaggct
    14821 ccggggataa tgatgttgtg gatttgatga aactagaaac atttcaggga ccgaccaata
    14881 cagatttact tgagtcggaa tggaatgctg actacgaagg aatctaccgt gccaatgttg
    14941 tattggaaaa ggtgccggga atagaaatgg acgaggtgtt gaaaaagaga atcctggcag
    15001 aagccaaatt tattcgagcc tggttttatt acaatctaaa taccattttt ggtggggttc
    15061 cactggcgga caaggtcctg gcgccatcgg aatacaacag accgaaggaa gatgccgctg
    15121 tcatttgggc attcatagag aaagatctga aagaggccat tgaagacctg cctcaaaggt
    15181 cacaatatca ggcgaaagac ttagggcgga ttacgaaggg agcagcaagg gccttgcttg
    15241 ccaaagcgta tctatatcaa ggtaagtatg cagaggcagg gtttcagaca ggagagatta
    15301 tccaatccgg agaatactcg ttggtagcag attatggaac gatatttacc aaggctggcg
    15361 agaacaatgc agaatcggtc tttgagattc aatatatgaa cgcatcgggg ggcaattggg
    15421 gtaaaaatgc ggccaatgaa ggtaccttta ccaatgtatt ccagcgtgcc agaggacaat
    15481 ttgccggcta tggcttcaat attccgacac aggattttgt tgatgaattc ttcaaagagg
    15541 gttttgagga tccccgcctt aaatatacgg t
//
LOCUS       DAOQMC010000023        15521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1374_length_15521_cov_5.229148, whole genome shotgun sequence.
ACCESSION   DAOQMC010000023 DAOQMC010000000
VERSION     DAOQMC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1374_length_15521_cov_5.229148"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1478)
                     /locus_tag="PKV31_01795"
     CDS             complement(27..1478)
                     /locus_tag="PKV31_01795"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HNB91613.1"
                     /translation="MSDKLNTSLLKGISEQPLENLAGLDDAAFDTISDIDKFLINDTL
                     LTYAIKGEEFPYELTAAGEEKFPVGPRCLIDLYHQMGYNSFASAGSLIIRRHLQLKCL
                     LQPPLIIPILEHAHDWSDYIFFLVKIIRERGRWLCEARTEWHWWKDALNPGIMEQKDN
                     VNRYLYFRLLRFFRYEEANSLLVNLWTNERKSTKQLFVDLLRENLSGGDVETLRYLWE
                     NESGKIRHEIRKLIFFASKNGEFFRLKKRLFSWEIKGTKAGEWMADLPIVAKFYDIDE
                     PFPAEWSAYVNFADKRKKKSGNDAFAAMPEKASERQRAFGDVLYATLVDGLFESLGRW
                     VSRIWSDQPAYRLGSDWIGYFNHLEEKKKNIVLQHIIHHGDDEATVRFSAQLMSRCLI
                     EMDKDSTSALLRLIKKVLQQPVCRLDDRWATEVKAYAPFVIHESYLGDWVDIGNLITR
                     KIVSAKTVNPSAETLKLIIFRRKIDTCFHESAK"
     gene            complement(1516..2082)
                     /gene="frr"
                     /locus_tag="PKV31_01800"
     CDS             complement(1516..2082)
                     /gene="frr"
                     /locus_tag="PKV31_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNB91614.1"
                     /translation="MNLEEANSILDKAKKMMDNAIEHLNHELSKVRTGKASPSLVAEI
                     KVEYYGSATPLSQVANVNISDAKTIVIQPWEKSMLRTIENALFEANLGITPQNDGEVI
                     RLTIPPLTEERRRDLVKQAKHLAEEAKVSIRNVRRDGMESIKKLVKDGFPEDLGKKKE
                     DELQKLTDHHTEQSGKLAEAKEKDIMAI"
     gene            complement(2173..3444)
                     /locus_tag="PKV31_01805"
     CDS             complement(2173..3444)
                     /locus_tag="PKV31_01805"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNB91615.1"
                     /translation="MKRIIFLGYSLLMVQLTIFGQGSWRKANDAPVFNRIDDIAFIND
                     SVALMGQDGKIYRSDDGGDNWILIGNLPHKAYVRSIEFLNDSTGFIGTIFDTKNGVAL
                     YRTDDGGKNWHSVSDRVQGGMYGICGLDHIGSTIIGVGIYSEPGRFYISRDGGDTWKS
                     YLIPESAALVDCVMLDENTFLVAGNSPDGRKGLILKSKDGGETWAEVAKSQYAMSFCW
                     KLSLRDDGLGLGSIEEAPTAFITHDHGDTWEEVLVDAQNKDTLRFGGAYFINEKLGWL
                     GIQWGMGTWETRDGGKNWKPIPFGGAINKIRGTNKGRALATGNTIYIYDGLSITKDQP
                     TDRPVRHSLKVSPNPAKDRIRLDIFLPNPTSLRLDLLDEQGYLIRQLFRGGVPAGNIS
                     KEYSIGNLSPGTYVIWMRTNEGHLSGKLIVY"
     gene            3902..4528
                     /locus_tag="PKV31_01810"
     CDS             3902..4528
                     /locus_tag="PKV31_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB91616.1"
                     /translation="MKKKYKTEQEDFWAGIFGDEYIHRNKSEEYLASNLNFFSKALNK
                     INKPESLIEFGANIGMNLRAIKLLFPKINIFGIEINGNAAEILSQFIGKEKVFNGSIL
                     EFVPEQKFDVVLIKGVLIHVDPDILHEVYAKLYESSKKYILIAEYYNPSPVTLNYRGH
                     SNRLFKRDFAGDMLDQYPDLRLIDYGFCYRRDNLFPQDDITWFMLEKS"
     gene            complement(4584..7595)
                     /locus_tag="PKV31_01815"
     CDS             complement(4584..7595)
                     /locus_tag="PKV31_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB91617.1"
                     /translation="MKNLMPLMTIAILISSHFNLLIAQPGNDELAWKISNYKEKRSQN
                     IHRIVEISDGNLLAVGYSSNHSEGGRDILLLKIDRNGKLLKAQTLGGRYDDECLSAKY
                     MPSGKILLCGYSKNKGLSQGIILLADEDLNTKLLYMDEKNENSLFKGIEVDRQGQIFV
                     CGQAGSKLVVKKFSPKGEFINQLYLTDEEGSNANALIFNRDGELYCTGSMANRKEFNR
                     SLMFLLKLDSNLNKIGLWNFPGKGFYEGNDLKENNVGELVIAGGGRHKNRGGSTPAIA
                     VVSTNGDLIRMETYPSFIDGIARGVAITPCGDILIAGYGSDLRNAQRTALFSVRINPG
                     NNYGTVWGPVFGGGSFSDEYNDVVCTSDGSVVFSGFTNNVLNQDYYIYTLKPVRRDLE
                     ELTILKNVEIMDSENEALNKNMETDLKITLRNNLSKDIKTFKVKIEIENNDKKGLILP
                     ESILVDRLRGGEEKEVNIPLVITDDFRDDYIDLKFIVEGGDRHQYNSRKAKIGVIGTY
                     ESSLVLVVDSSRADIADKDRIVLPVSLRNLGDAPALVRDMDFRFNENVSLADDYEAPV
                     NTVIQVGEELRFNLICKVDSLFADDYIVANIKLRELSGYRIQIETPRSLTLRKMIPVI
                     DDNSQLYDQPIILSNEATEISFTNMAETVRLIDRKNYHVNFDCNLTPSEIASNFKFII
                     NGVEIAKDSLKIGYKENNYSVAVPLTLGDNELILERDAGVRDTLGKINVASSNVNYYI
                     YIIGTDLRDEDNMPVVRYSIKDVNDFYNKIKLLGKSKNTKVNIKLFNTFSLTTSNNLK
                     GVFNELLARKHKFKANDVMIIYWVGQGFATAKGDIYVKGSELLKNRKNKFMPENYSFP
                     LTSIYIPYFLGELPCKKIMLLDIFNKEDPESTGNDDDEGQDYSLIDQTVSAIKTFRNF
                     SYILSGNPGQKSYELQKVENGAFTYAVIQALEGNKGNLIGDNVMALSDFFENISRSIP
                     DLVVKNGYKTWKQNPMYIKSRPEEDVVIFYK"
     gene            complement(7598..8968)
                     /locus_tag="PKV31_01820"
     CDS             complement(7598..8968)
                     /locus_tag="PKV31_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB91618.1"
                     /translation="MMGQTPPKIVFRKNIKLKPQPVKQEKKNKLSFYIKWSVWSLVTF
                     VALFIFYRIGVFGKPEISPKKSTVSPPALTDTLESAQESSEDSIYSIIYNSNFFNYRI
                     VPDILEEDMNADDFFGMFHIHPEKKAKAEMLLGANARFTEGTRIVTLYSKDDEEIPLY
                     IYIEPDPSRYRYISFEKDIPEISNYIKRNLTIETEKRKIVIGKDALENITEQAPAELL
                     GRISHVMSWKKDIGKLDEGDVVKVAYEGLYYNKIFMGVGKLMALEWKGKEDSLKAFLV
                     SGEYDNEGRSVYCTEKGRPLKEAFRKSPVAYGRISSRFGIRLHPVRKVVKAHNGTDFA
                     SPEGSPIFAIGDGVVTVAAFKKANGNYIKIMHDKTYTTQYLHMSRFKPGIVAGKRVRQ
                     GDVIGYVGHTGEATGPHVCLRLWKNAVQVDFLKEKFPQQGKIKAFDAKVFQSARDYYS
                     KQLENI"
     gene            complement(8974..9204)
                     /locus_tag="PKV31_01825"
     CDS             complement(8974..9204)
                     /locus_tag="PKV31_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91619.1"
                     /translation="MENKTIQRASLESLDELSGFNTGESEGSFLKPPEFNLTAKLHNT
                     GMSPYEDRPVDDVGNRFAFGRSADSTSKKNIT"
     gene            complement(9210..10205)
                     /locus_tag="PKV31_01830"
     CDS             complement(9210..10205)
                     /locus_tag="PKV31_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNB91620.1"
                     /translation="MKLPVTFGFLTTCILFNLVIFSSECQAQQLPLFTQYREVQGLIN
                     PASISHDYFLEKYNANLGITYRNQWTDINGHPVTAAIKADYIVVPANSSFSLLTGAYY
                     LSDRAGLTNRQSAYVRLGSIYSQNPSESGFGLGINIGQVWNSFNFGEVKLTDPQDINI
                     DKVSNFNYLDLGIGTYYYKKLKSGPLKNDQFYVGVSVPQIFNQDYVIKNGTNEFVVKR
                     VPHIYAHAGYYKTMSETMYIAGSFWYRYVHGGGSIADINARIYLFDKLWFGAGASTAG
                     TLHVEAGTILGKYFNFNDKFNLKIGYASDFNLGYLAKNLGLSHELNLILFLDTVN"
     gene            11143..13212
                     /locus_tag="PKV31_01835"
     CDS             11143..13212
                     /locus_tag="PKV31_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB91621.1"
                     /translation="MEKTKDSILIDREIYLKALETMFSAKFMSEWYEENFKFVSKYVH
                     ATSRGHEAIQIACGLQLKPVDYVYPYYRDDAMMLAMGVTPFQMMLQLMAKRDDPFSGG
                     RSYYCHPSLRDADKPQIPHQSSATGMQAIPATGAALGMWYKEQQGMDQGDIPPLVVCS
                     IGDAAVTEGEVSEAFQMAALKSLPIVFLVQDNKWDISAFVDEVRSWNAAEYVRGIRGL
                     DSVSIDGSDFNESYDTLRHVFSIVRKERRPFVVHARVPLLNHHTSGVRKEWYRFDLEE
                     AVLDDPYPKLTTFLLEEGLISQRELEVLESRIRISIKQQYEQASNSEDPKPEDLYTHI
                     FAPTPITEEQGNRHGKDNTVKNMVDSGIFAIRELMLEDERCVLYGQDVGARLGGVFRE
                     AATLGEQFGSHRVFNTPIQEAFIIGSTVGMSAVGLKPIVEVQFADYIWPGLNQLFSEV
                     SRSNYLSNGKWPVSMVLRVPVGAYGSGGPYHSSSVESIVANIRGVKVVYPTDAADLKG
                     LMKAAYYDPNPVVIFEHKGLYWAKLPGTENAKCIEADSDYVVPIGKANILVESKDIND
                     QPTCTVIAYGMGVHWAFNAEKAFPGRVEIIDLRSLVPLDTETIYQSVNKTGRCLVITE
                     EAVDGSYAQSIAARVQENCFTALDAPVRTIGSENVPAVPLNETLEKTMLLNIDKVTKA
                     IESLLVY"
     gene            13226..13801
                     /locus_tag="PKV31_01840"
     CDS             13226..13801
                     /locus_tag="PKV31_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91622.1"
                     /translation="MLRQLLKLTFVFALLLTGLNLSAQKNVVFELDGGYSMIGDNSQT
                     GFGLNADLGYTFWGRLTPTVGYQFHTFANQRNHYDTNANIDLAYLAVKVTALKFKWFS
                     LDLGPLAFRRMWKQEYKVHDGYTVYKDGLTMDGNNIYSYYKPQWGYGGVAGVKFDLFK
                     KSGVKLDVTYQIDTDRKDSMMFYSGGVFFRF"
     gene            14294..15514
                     /locus_tag="PKV31_01845"
     CDS             14294..15514
                     /locus_tag="PKV31_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91623.1"
                     /translation="MKKLFLILFVFVLNCPVFSQDPVFTLVKVGFDNFNFFYDIKASP
                     EGTLWVCSNEGNVFRKLSSSTTWEHISDYPGTGAEAKALFVAADDDVWIISYGNGIFH
                     FNGTKWEEVSLTNVPETNKWNCVSGDINGNVWFGSNDKGLYKYSAGQWTNYSMSNSGI
                     LSDQINDLLATPSGKLFVGGYGTLQTFESNNWVDLGFTSNIGFGERIYSFAIDNDGLV
                     YIATTKGLFKFKDDKLEIYGDTKNRDVYYAVFDRNNVLWFFGEEQAFDNYLYRCDNGT
                     CKKLDDKNLPHQCSGLAVGSDNLLYLIGSLGPNILVIEDKTTNSDKELLRQPMVEISP
                     NPASDFLSIKSPQTLKEISLFNSAGQSLFAAKNINQTHFMLNLQGRGKINPGVYVLLI
                     KTEIGTCRKKIMIQ"
BASE COUNT         4471 a         3373 c         3010 g         4667 t
ORIGIN      
        1 agcgaacttc ggcactcggc ctgatttcat tttgctgatt catggaagca ggtatctatt
       61 tttctcctaa aaataatcaa ttttaatgtt tctgctgatg gattcaccgt tttggcactt
      121 acaatcttcc ttgtaatcaa gttgcctata tctacccagt cgcccagata actctcatgg
      181 attacaaatg gggcataggc tttcacttcc gtggcccacc tatcatccag acgacatacg
      241 ggctgttgca gaaccttctt tataagtcgt aacaaggcgg atgtgctatc cttatccatt
      301 tcaatcagac aacgagacat caattgcgca gaaaaacgta ctgtagcctc atcatcacca
      361 tggtggatga tgtgttggag cacaatattc ttttttttct cttccagatg attgaaataa
      421 cctatccagt ctgatcccaa ccgataagca ggctgatctg accaaatcct gctaacccat
      481 cgtccaaggc tttcaaacaa accatcaacc aacgtggcat acaacacatc accgaaggct
      541 cgctgacgct cagatgcttt ttcaggcata gcggcaaatg catcatttcc ggatttcttc
      601 tttcttttgt ctgcaaagtt tacataggcc gaccattcag caggaaatgg ttcatcgata
      661 tcataaaatt tagctactat cggcaaatct gccatccatt caccggcttt tgtccctttt
      721 atctcccatg aaaagagcct ttttttaagc cggaaaaatt ccccattttt ggaggcaaaa
      781 aatatcaatt tacggatctc atggcggatt tttccacttt cgttttccca caagtacctg
      841 agagtctcta catctcctcc tgacaaattc tccctcaata aatcaacaaa aagctgtttt
      901 gtcgatttac gctcgttggt ccacaaattt accagaaggc tgtttgcctc ttcgtacctg
      961 aaaaatcgaa gcaatcggaa atacagatat cggttgacat tgtccttttg ttccatgatg
     1021 cccggattga gagcatcttt ccaccaatgc cattcagtcc tggcctcaca taaccatcga
     1081 ccacgttctc ttatgatttt taccaaaaaa aagatgtaat cggaccagtc atgggcatgt
     1141 tccagaatcg gaatgatcaa gggcggttga agcaggcatt tcaattgcag atgccggcgg
     1201 atgatgaggg atccggcact tgcaaagcta ttatatccca tttgatgata cagatcaatc
     1261 aggcagcgtg gaccgaccgg aaacttttcc tctccggcag ctgtcaattc atacgggaat
     1321 tcttcccctt taatggcata ggttagcaaa gtatcattga tcagaaactt atctatatcg
     1381 gatattgtgt cgaaggcagc atcgtccagg cctgcaagat tctccagcgg ctgttcagaa
     1441 atacctttca gcaaggatgt atttaacttg tcggacatga caattctaat ggaaggtgac
     1501 tgaaggaaat cggcattaaa tcgccatgat atccttttct tttgcttctg ccagcttgcc
     1561 ggattgttcg gtatggtgat ctgtcaactt ctgcaattca tcttccttct tcttgcccag
     1621 atcctccgga aaaccatcct tgaccagctt cttgatactt tccatcccat ctcttcgcac
     1681 attgcggata cttacttttg cttcctccgc aagatgtttg gcttgcttca ccaggtcacg
     1741 ccggcgttct tctgtaaggg gtggaatagt caggcggatg acttcaccgt cattttgcgg
     1801 tgtgatgccc agattagctt caaacaaggc attttcaatt gtacgcaaca tagatttctc
     1861 ccagggctgg ataacaatag tcttggcgtc tgaaatattg acattggcaa cctggctcaa
     1921 aggagttgca ctgccgtaat attctacctt aatctcagca actaaagatg gggaagcctt
     1981 cccggtcctt acctttgaca attcatgatt aaggtgttcg atggcattat ccatcatttt
     2041 ttttgccttg tcaagaatgc tatttgcctc ttccaaattc ataatttaaa catttttcgg
     2101 acgtaaaaat atattttaaa actgaaaaaa gaaaattacc gcttaaaatg attcatatct
     2161 tgccccttgc catcagtaga caataagctt accggacaaa tggccttcat tggtacgcat
     2221 ccagataacg tatgttcccg gagataaatt ccctatggaa tattccttgg aaatattgcc
     2281 cgccggcacc ccgccacgga acaactgacg tatgagataa ccctgctcat ccaacagatc
     2341 aagtctcaga gaagtaggat tggggagaaa gatgtcgagc cggatacgat ccttagccgg
     2401 attgggggat accttgagtg aatgcctgac aggccgatct gtcggctggt cctttgtgat
     2461 gctgaggcca tcgtagatat atatcgtatt gcctgtagca agagctctcc ctttattagt
     2521 tccacggatt ttattgatgg ccccgccaaa aggaatcggc ttccagttct taccaccatc
     2581 gcgggtctcc caggtgccca ttccccactg gataccaagc cagcctaact tttcattgat
     2641 gaaatatgct ccgccaaatc tcaatgtgtc cttattctgt gcatcgacca ggacttcctc
     2701 ccaagtatca ccatgatcat gagtaatgaa ggcagtaggc gcttcctcaa tggagcccaa
     2761 gcccagacca tcatcccgga gcgacaattt ccagcaaaag ctcatagcat actgagactt
     2821 ggccacttct gcccaggtct ctcctccgtc ctttgacttc aggatgagac ctttcctgcc
     2881 atcaggactg ttacctgcga caaggaaggt gttttcatcc aacatgacac aatccaccag
     2941 tgcggcactt tccggaatta aataactttt ccaggtatct ccaccatccc ttgaaatata
     3001 aaaacggccc ggttccgaat aaattcctac accgataata gtacttccga tgtgatccag
     3061 cccacagata ccatacatgc ctccttgcac cctatcactg accgagtgcc agtttttacc
     3121 cccatcatct gtacgataaa gtgccacacc attctttgtg tcaaagattg tgccgataaa
     3181 tcctgtactg tcattgagaa attcgatgct acgtacatag gccttatgag gaagattgcc
     3241 gataagtatc cagttgtctc ctccatcgtc cgagcggtat atctttccgt cctgacccat
     3301 aagggccaca gagtcattga tgaaagcaat atcgtcaatc cggttaaaca caggtgcatc
     3361 attggcctta cgccacgaac cttgtccaaa aattgtaagt tgaaccatca aaagactgta
     3421 gcccaaaaaa ataattcttt tcattgtcat ccattcattg ggttggaaaa tatatcctgc
     3481 gaatcacggc gcaaatttag agaaaaacgt tgatttgatt gacggataaa gaaataagtc
     3541 ggcaataggg ataacgatgt gctaatcctg attggcttgc ctgatatgcg tccggataac
     3601 gggaaattga caatttacac cttcctttaa attctttaca attattcatt gaaaaattac
     3661 ttttaggcta caaaaaccaa tccaacacca ttgattaaat actaaaggcg cataataaca
     3721 aatcgaatat gttggaattt tgtacatttg gtcttgcatg ctgtattccg ctttcgatat
     3781 gaaaatttgg gtatttgatt gtatgaatat ggaatattga aatacatgtt gccgagaaat
     3841 aaaaaatggc accaggtgat tggaaaacga aataatcata ttaaaacgaa cggtccgaaa
     3901 aatgaaaaaa aagtataaaa ccgaacagga agacttctgg gccgggatat ttggggatga
     3961 atacatacac aggaacaaaa gtgaagaata cctggcctcc aacctgaatt tcttttcaaa
     4021 ggcattgaac aaaatcaaca aacctgaatc tctgatagag tttggagcga acattggaat
     4081 gaaccttcgg gcaattaagc ttctgttccc gaagatcaat atattcggta tcgaaataaa
     4141 tggaaatgcg gcggaaatat tatcccaatt tatcggtaag gagaaggtat tcaatggatc
     4201 tattctggaa tttgttccgg aacaaaagtt tgatgtagtg cttatcaagg gagttttgat
     4261 ccacgttgat ccggatatac ttcatgaagt atatgccaag ctctacgaat ccagcaagaa
     4321 atatatccta atagcagaat actacaaccc ctccccggtc acattgaatt atcgtgggca
     4381 tagcaatcgg cttttcaagc gggactttgc cggtgatatg cttgatcaat atccggattt
     4441 gagactcatc gattatggct tttgttacag aagggataat ttgttccctc aggacgatat
     4501 aacgtggttt atgctggaaa aatcctgaaa tttttaaaca ggtattttgc ctgacagata
     4561 ttcaataatc caatataatc atattacttg taaaaaatca cgacatcttc ttccggtctg
     4621 gatttgatat acatagggtt ttgtttccag gttttataac cattcttgac aaccaaatcc
     4681 ggaattgatc tggaaatatt ttcaaagaag tcagataggg ccattacgtt gtctcctatc
     4741 agattccctt tgttcccttc caaagcctga ataacggcat aagtaaaggc tccattttcg
     4801 accttctgta attcatagct tttttggccc ggatttccgg aaaggatgta actgaaattt
     4861 ctaaaagtct tgatggcgct gacagtttgg tcaatcaggc tgtaatcctg cccctcatca
     4921 tcatcatttc ctgtgctttc cggatcttcc ttattaaaaa tgtcaaggag cataatcttt
     4981 ttgcaaggca actctccaag gaaatatgga atatatattg atgtcaaagg aaaactatag
     5041 ttttcgggca taaatttgtt cttcctgttt tttaaaagct ccgacccctt tacatagata
     5101 tctcctttag ctgttgcaaa gccttgccca acccaatata tgatcattac atcatttgcc
     5161 ttaaacttat gtttccgggc taataattca ttaaaaacgc ctttcaaatt attagaagta
     5221 gtcaggctaa aagtattgaa cagtttaatg ttgacttttg tattttttga cttgccaagt
     5281 aattttatct tattgtaaaa atcattgaca tccttgatac tgtaccggac taccggcata
     5341 ttgtcttcat ctctgagatc ggtcccgata atgtatatat aataattgac gttactactg
     5401 gcaacattta tcttgccaag agtatctctg acaccggcat ctctttccaa tatcaattca
     5461 ttatctccga gagtcaacgg aacagcaact gaataattat tttccttgta accgattttt
     5521 aaggaatctt ttgcaatttc tactccattg atgataaact tgaaattaga agctatttct
     5581 gaaggagtta gattacaatc gaaattgaca tgataatttt ttctgtcgat cagcctgaca
     5641 gtttctgcca tattggtaaa tgatatttcg gttgcttcgt tgcttaaaat tatcggttgg
     5701 tcataaagtt gtgaattatc atcgatcacc ggaatcattt tccttaatgt aagactcctg
     5761 ggggtctcta tttgaattct atatcctgac aattctctca atttaatatt cgcaacgata
     5821 taatcatctg caaaaagaga atctacctta caaatcagat taaatctaag ttcctcgcct
     5881 acctgaatga cagtattaac aggagcctca taatcatccg caaggctcac attttcatta
     5941 aaccggaagt ccatatccct aaccaaggca ggtgcatcgc ccaaattacg aagggagaca
     6001 ggcaatacaa tccgatcttt atccgcaata tcagctctgg acgaatctac tacaagaacc
     6061 agggatgatt cgtatgtccc gatcacgcct atctttgctt tccgggaatt gtactgatgc
     6121 cggtctccac cttcgacaat aaacttcaaa tcaatatagt catcccgaaa atcatctgta
     6181 ataaccaatg gtatattgac ttccttttcc tctccacccc gcagtcggtc aaccaatatc
     6241 gactccggca aaatcaaccc ttttttatcg ttattttcta tttctatttt taccttaaat
     6301 gttttgatgt cttttgacaa gttgtttctg agtgtaatct tcaggtcggt ctccatattc
     6361 ttattcaatg cctcattctc tgaatccatg atttcgacat tcttaagaat cgtcaattcc
     6421 tccaaatcac gacgtaccgg cttcaaagta tagatataat aatcctgatt caatacattg
     6481 ttggtaaaac ctgaaaaaac aacactgcca tcggaagtac atacaacatc gttgtattcg
     6541 tctgaaaaac tgccacctcc aaaaacaggc ccccaaactg tgccataatt attaccggga
     6601 ttgatccgaa cagaaaagag tgctgtcctt tgagcatttc tcaaatcgga accatacccg
     6661 gcaatcagaa tatctccaca aggagttata gcaacaccgc gtgcgattcc atcgataaag
     6721 gaaggatatg tctccatcct gatcaggtct ccattggttg aaacaactgc tattgcagga
     6781 gtagaacccc ctcggttttt gtgtcgtcct ccacctgcaa tcaccaactc gcctacatta
     6841 ttttccttca aatcatttcc ttcgtaaaaa cccttacctg gaaaattcca taatccaatt
     6901 ttatttagat tggaatccaa ttttaaaaga aacatcaaag acctgttgaa ttcctttctg
     6961 tttgccatac ttcctgtaca atacagctct ccatctctgt taaaaatcaa agcattggca
     7021 ttagaacctt cctcatcagt caaataaagc tggttgatga attccccttt agggctgaat
     7081 tttttgacta ccaacttact gcctgcctgt ccgcaaacga agatttgacc ctgccggtca
     7141 acctcaattc ccttgaatag tgaattttca ttcttttcat ccatatacag cagcttagta
     7201 ttcaaatctt catctgccaa caagattata ccttgtgaaa gacccttatt tttcgaataa
     7261 ccgcataaca gtatttttcc tgatggcata tacttagctg aaagacactc atcatcataa
     7321 cgtccaccca gggtctgcgc ttttaacaat ttgccattcc ggtctatttt caataaaaga
     7381 atatctcttc ccccttcgga atggttggac gaatagccca cggctaaaag attaccatcg
     7441 cttatctcaa caattctatg gatgttctgg cttcgttttt ctttgtagtt tgatattttc
     7501 caagctaatt catcattacc tggctgggcg atcagtaagt tgaagtggga tgaaatcaaa
     7561 atcgcgattg tcatcaatgg cattaaattt ttcatattca gatattttcc aattgttttg
     7621 aataataatc tctcgcactc tgaaatactt ttgcatcaaa ggctttgatt ttgccttgtt
     7681 gaggaaattt ttccttcagg aaatcgacct gtacagcatt cttccacaat ctcagacaaa
     7741 catggggacc ggtagcctca ccggtatggc caacataacc aataacatca ccttgcctga
     7801 ctcttttccc ggcgacaatt cccggcttaa atctggacat gtgcaaatac tgggtggtgt
     7861 aggtcttgtc atgcatgatc ttgatataat ttccattggc tttcttaaag gcagcaacag
     7921 taacaacgcc atctccgatt gcaaagatgg gagacccttc cggagaggcg aaatccgtac
     7981 cattgtgtgc tttgactact ttacggaccg gatggagtct tattccaaac cgggaagaga
     8041 ttcttccata tgcgaccggt gactttctga aggcctcttt aagggggcgc cccttctcgg
     8101 tgcaatagac cgacctcccc tcattatcat actcaccgga aacaagaaag gctttcagac
     8161 tgtcctcttt tcccttccac tcaagtgcca tcaatttacc gacccccata aatattttat
     8221 tatagtaaag accttcataa gcgactttaa ccacatcacc ttcatccaat ttcccaatgt
     8281 cctttttcca gctcatcaca tggcttattc ttcccaataa ctcagcaggg gcctgctctg
     8341 taatattttc caaagcgtct ttacctatga caatctttct cttttccgtc tctatagtca
     8401 gattgcgttt tatatagttt gatatttccg gaatatcttt ttcaaaggaa atgtatctgt
     8461 atcttgaagg atccggttcg atatagatat ataagggaat ttcctcatca tccttgctgt
     8521 ataaagttac tatcctcgtc ccttcagtaa atctggcatt cgcgcccagc agcatttccg
     8581 cctttgcttt cttctcaggg tggatatgaa acatcccgaa aaaatcatct gcattcatgt
     8641 cttcttcgag tatatcgggt actatccggt aattgaaaaa attggaattg tatatgatgg
     8701 aataaattga gtcttcggat gattcctgag ctgactccaa agtatccgtc aatgcaggtg
     8761 gactaacggt agattttttt ggggaaattt caggtttccc gaaaacccct attctgtaaa
     8821 aaataaacaa tgccacaaaa gtaaccaaac tccatacact ccatttgata taaaacgaaa
     8881 gcttgttttt tttctcctgc ttgacaggtt gtggcttaag ttttatattc tttcgaaaaa
     8941 ctattttagg cggagtctga cccatcattc atgtcaagta atattctttt tgcttgtact
     9001 atctgcagac cgaccgaatg caaaacggtt gcctacatca tcaaccggcc tatcttcata
     9061 tggagacatc ccggtattgt ggagtttagc tgtgaggtta aattcaggag gttttaagaa
     9121 actcccttca gactccccgg tgttaaaacc ggataattca tccagggatt ccaggcttgc
     9181 tctttgaata gtcttgttct ccatagtatt caatttacgg tatccaaaaa caggataaga
     9241 ttcaactcgt ggctcaggcc caggttctta gcaagataac caagattgaa atctgaagca
     9301 tacccaattt tcaagttgaa tttatcatta aaattaaaat atttacccag gatagttccg
     9361 gcttcgacat gaagggttcc ggctgtcgac gccccggcac cgaaccacaa cttatcaaac
     9421 agatagatgc gggcattaat atcagcgatg gatcccccac cgtggacata tctgtaccaa
     9481 aaacttcctg caatatacat agtttcagac atagtcttat aatatcctgc atgtgcatag
     9541 atatgcggaa ctctcttgac cacaaattca ttcgttccgt tcttgataac ataatcctga
     9601 ttaaatattt gtggaaccga tacaccgaca tagaattgat cgttctttaa aggcccggat
     9661 tttaattttt tgtaataata ggtgccgatg ccaagatcga gatagttaaa gttacttact
     9721 ttgtcgatgt tgatgtcctg tggatcagtc aatttgactt cgccaaaatt aaatgaattc
     9781 catacctggc caatattaat acccagccca aatccactct cagaagggtt ttgcgaataa
     9841 atagagccca atctaacata agcagactga cgattggtca accctgcacg atccgacagg
     9901 taataggcac ctgtaagcag gctgaaggag gaattggccg gaacaacgat atagtcagcc
     9961 ttgatggctg cagttaccgg atgtccattg atatctgtcc actgatttct atatgtgata
    10021 ccaagattgg cattatactt ttcaagaaaa tagtcgtggc taatggaggc aggattaatc
    10081 aagccctgaa cttcacgata ttgcgtaaaa aggggtaact gctgtgcctg gcattccgat
    10141 gaaaatatca cgagattgaa aagaatacag gtggttagga atccaaaagt cactggtaat
    10201 ttcataatct ttatatttaa agtttaaagt aaattcttga cctgccaatg ctgatcaatt
    10261 ctgtatattt taatagttca gaacatcact tcttaaaccc attcccttta aaccggtgaa
    10321 attcataatc ctgcttcttc attgattttg aaaagcttat tccttcccaa taatccagat
    10381 ttcaccattc cattttcaag aacccttctt tcagtctatc acttaccatt ctataggtta
    10441 atgtgcttca tttaccgatt acctatattc cggattcctg aacctgagtg ccaataagaa
    10501 tacctcatat ccccttgata cccatatgct tttttccggt tgctgatact tctttcctaa
    10561 aaggactttc ccggttcgat ccatcataaa acacctaatg actcaccaca taatctgctc
    10621 gaatacattt ctgtatcgtt tcacttccag gccgggagct taatgaacaa aattacattt
    10681 taaattggaa ttaataaaca aaaaatatag acattaagag tttattccca taatagaata
    10741 tttcctttaa tccaaataat tcaaaacgct gctcaacgac gattgactca aaaatcaatt
    10801 tgcccggtac gcggctatgt catttgtctt tacttcaatc gaacgggcat ttgtccatca
    10861 tacatgcata accataccta aactttcaaa cttgagcaac tttgctattt attgacaccc
    10921 tcatcacatc cactactgca gcaacatatt cactaggcta atcttgtttt ggtttatgcg
    10981 cctattaaat tgtaagcgca tacctggccg gcccgaccaa gcgcttattc cttactaatg
    11041 cttgctatct caagctgtac aatttgtgaa atacgcttat caaaagcatc catcagggtt
    11101 gaaatctttt tctatctttg tgttctatta aattattatt tcttggaaaa gacaaaagac
    11161 agcatactca ttgacaggga aatatacctg aaggcattgg agacgatgtt ttcagcgaaa
    11221 ttcatgtcag agtggtatga ggaaaacttc aagtttgttt ccaagtatgt ccatgccact
    11281 tctcgcgggc acgaagcgat acagattgcc tgtggattac agcttaaacc tgtggattac
    11341 gtctatcctt attatcggga tgatgcgatg atgctggcaa tgggggttac accctttcag
    11401 atgatgctac agctgatggc taagcgggat gatccgtttt caggtggccg atcgtattat
    11461 tgccatccga gtcttcgcga tgcggacaaa ccacagattc ctcatcagtc atcagctacc
    11521 ggtatgcagg ccataccggc tacaggagct gcgctgggca tgtggtataa ggagcagcaa
    11581 gggatggatc agggtgatat tccgccattg gtcgtgtgtt ccatcgggga tgcagccgtg
    11641 acggagggtg aagtaagtga agctttccag atggcggcct tgaagagctt gccgattgta
    11701 ttcctggtcc aggataacaa atgggacata tcggccttcg tggatgaagt gcgcagctgg
    11761 aatgcagcag aatatgtaag aggaatccgg ggactggatt ctgtttcgat cgatggttct
    11821 gatttcaacg agtcgtacga tacactcaga catgtatttt ctatcgtcag aaaggaacgg
    11881 cgaccttttg tcgtacatgc cagggtacca ttgcttaatc accatacttc gggagtgcgt
    11941 aaggagtggt atcggtttga tcttgaagaa gctgtacttg acgaccctta tcccaaactg
    12001 actactttcc tgttggagga gggattgatt agtcagaggg agttggaagt actggagtcc
    12061 cgtatcagga tttcgatcaa acaacaatac gagcaggcaa gcaatagcga agacccaaag
    12121 cctgaagacc tgtatacaca tatcttcgct cctacaccca ttacggagga gcaaggcaac
    12181 aggcacggca aggataatac tgtcaagaac atggtggata gcggaatatt cgccatccga
    12241 gagctcatgt tggaagacga acgttgtgtc ctttatggcc aggatgtagg tgccagattg
    12301 ggaggtgtat ttcgtgaggc agctacactt ggggaacaat tcggaagcca tcgtgtattc
    12361 aatactccca tccaggaagc attcatcatc ggaagtactg tgggcatgtc ggcagtaggc
    12421 cttaagccca ttgtagaagt gcagtttgcc gattatatat ggcccggact taaccagttg
    12481 ttttcagaag tcagtcgatc caactacctg tccaacggaa aatggcctgt cagtatggtg
    12541 ctcagggtgc ctgtaggggc atacggcagc ggtggacctt accactccag cagtgtggag
    12601 tctatagtag ctaacatacg aggggtgaag gtcgtgtatc caaccgatgc agcagacctg
    12661 aaaggattga tgaaagcggc ctattacgat cccaatcctg ttgtgatatt cgaacacaaa
    12721 ggcttatact gggctaaatt gccgggtacg gaaaatgcta aatgtattga ggcagacagt
    12781 gactatgtgg tccctatcgg caaggcaaat attttagttg aatccaaaga tatcaacgat
    12841 cagccaactt gtacggtcat tgcctatggc atgggcgtgc actgggcgtt taatgcagaa
    12901 aaagccttcc cgggaagggt ggagatcatc gacctgagat cgttggttcc attggatacg
    12961 gaaacaatct atcaatcggt aaacaaaaca ggccgttgtc tcgtcataac agaagaagct
    13021 gtcgatggct cgtatgctca aagtatcgct gccagggtcc aggaaaattg tttcactgcc
    13081 cttgacgcgc ctgtacgtac aattggctcg gaaaatgttc cggctgtgcc attgaatgag
    13141 acgttggaaa aaaccatgtt gttgaatatc gacaaagtca ccaaagctat tgaatcattg
    13201 ttagtttatt aaaattctaa aataaatgtt acgtcaatta ctaaaactca cttttgtatt
    13261 tgccctgttg ctaacaggtt tgaatctttc cgctcaaaaa aatgtcgtct ttgaattgga
    13321 tggcggatac tccatgattg gagacaacag ccagaccggc ttcgggctaa atgctgacct
    13381 tggatatact ttttggggtc gtttgacacc tactgtcggt taccagtttc acacctttgc
    13441 caatcagcgt aatcattatg ataccaatgc caacatcgat ctggcatacc ttgcagtcaa
    13501 ggttaccgca ttgaaattca agtggttttc ccttgatcta ggccctttgg cattccgtcg
    13561 catgtggaag caggagtata aagtacatga tggatacaca gtctataagg acggtctgac
    13621 catggatggc aacaatattt attcctacta caagcctcaa tggggctatg gtggtgttgc
    13681 cggtgtaaaa ttcgatctat tcaagaaatc cggtgtcaag ctggatgtta cttatcagat
    13741 cgataccgac cgtaaggata gtatgatgtt ttactcaggc ggtgtattct ttagatttta
    13801 aggagtcaga aaccctctgt ctatcgccaa tcgacggatg atattcatcc gtaaagtcgg
    13861 ttgtcaggct gtaatctttt aagatggcag acaagtattg gcagaatata atacaagtca
    13921 ttggcgtcca acagtacaaa gaaaggaata ttctcctgac ttcggcattt agtaaattat
    13981 gaaaattata aatcgctaat aatccatagc ttggcaaccg atcgggcaac aacacgtctt
    14041 tttggggtgt acatcccggg atcaacttgt gtatccggtt gataggatga ggtgtttcca
    14101 ccgggtatgc atggctagtt gataacatcc gttctaaaat aacgggcaaa tcatcctctg
    14161 tcttatctta caatccccgg taagcagatt ttgcagttta atgaaaggaa taatgcatga
    14221 tgaattataa aattcaaatt attattgata tatttgaatc tctcattata caataactaa
    14281 aaatcaatta attatgaaaa agctatttct tattctcttt gttttcgttt taaattgtcc
    14341 cgttttctca caggatcccg tgtttactct tgttaaagtc ggctttgaca attttaattt
    14401 cttttatgac ataaaagcga gtcccgaagg aacattatgg gtgtgttcta atgaagggaa
    14461 tgtattcagg aaattaagtt cctctacgac ctgggagcat attagtgact atccgggaac
    14521 tggtgccgag gcaaaagcat tatttgttgc tgcagatgat gatgtttgga ttatttcata
    14581 tgggaatggt atttttcatt ttaatggcac aaaatgggaa gaagtctctc ttaccaatgt
    14641 tcctgaaacc aacaaatgga attgtgtttc aggagatata aatggaaacg tatggttcgg
    14701 gtccaatgat aagggccttt ataaatatag tgccgggcag tggacaaatt acagtatgtc
    14761 caattcgggt attttgtcag atcaaataaa cgatctgtta gcgacacctt cgggtaagtt
    14821 gtttgtggga ggttacggaa ctttacagac ttttgagtca aataattggg tagatttagg
    14881 ctttacttcc aatattggtt ttggtgaacg catctatagt tttgccatcg ataatgatgg
    14941 cctggtctat attgccacaa ctaagggact atttaagttt aaagatgaca aattagagat
    15001 ttacggagat accaaaaata gagatgttta ttacgcggtt tttgaccgta ataatgtttt
    15061 gtggtttttt ggtgaagaac aggcttttga taactacctg tatcgctgtg acaatggaac
    15121 ttgcaagaaa ctagacgata aaaatttacc tcatcaatgt tcgggtctgg ctgttggaag
    15181 tgataattta ttatatttaa tcgggtcttt aggacccaat atcttagtta ttgaagacaa
    15241 gacaacgaat tcggataaag aattattgag acaaccaatg gtagagattt ctcctaatcc
    15301 cgcttcagat tttttatcaa taaaatcccc gcaaacgttg aaagaaattt cactatttaa
    15361 tagtgcaggt caaagtttat ttgcagctaa aaacattaac caaacccatt ttatgctgaa
    15421 cttacaggga cgtggtaaaa taaatcccgg agtgtatgtc ttattgatta agacagaaat
    15481 tggaacttgc agaaaaaaga taatgattca ataaatagtt a
//
LOCUS       DAOQMC010000024        15120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1432_length_15120_cov_5.127580, whole genome shotgun sequence.
ACCESSION   DAOQMC010000024 DAOQMC010000000
VERSION     DAOQMC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15120
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1432_length_15120_cov_5.127580"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKV31_01850"
     CDS             <1..249
                     /locus_tag="PKV31_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91624.1"
                     /translation="GKIDNLQVYPNPVNNVVNIAGVAPNSVIKIFDMAGNQVNRKFTL
                     SAMNSFDLSACKEGIYLIVVSDGSGNTETFKILKNKVP"
     gene            441..1115
                     /gene="rpe"
                     /locus_tag="PKV31_01855"
     CDS             441..1115
                     /gene="rpe"
                     /locus_tag="PKV31_01855"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNB91625.1"
                     /translation="MSGLIAPSILSADFTKLADEISMINRSEADWFHIDVMDGVFVPN
                     ITMGFLVVKAINQLAEKPMDVHLMTVHPELYIEQWRDAGADHITVHYEACTHLHRVIQ
                     QIKHTGAKAGVAINPHTPVKLLEDIIEDLDLVIVMSVNPGFGGQAFIPRTFDKVRALS
                     AMIRERNASTLIEIDGGVTLANAGALIESGAHALVAGNTVFNSPNPTETIHLLKKTCS
                     HTKHNA"
     gene            1112..3673
                     /locus_tag="PKV31_01860"
     CDS             1112..3673
                     /locus_tag="PKV31_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB91626.1"
                     /translation="MMSLDRELNPIRMPLFIRLFFPVFFLLLCFKLDAQETSTLVLGK
                     IIDEISGEPVEAALIYEKSEQIGVESDVNGFFQLSVPAGKPLTIIIRRVGYETAAIKL
                     NSITEGTIKKLDISLIQKDAGQGVTIYAESIGDKNMVRANVKEFKLLPTVSGNLESVL
                     PSIALGTSSGSGGELTSQYNVRGGNYDENLVYVNDFEIFRPQLIRSGQQEGLSFPNID
                     LIRDLAFSSGGFEAKYGDKMSSVLDIKYKRPEERRGSVSGSLLGATAHLEGLMKPFKN
                     RGGQLRYLLGARYKTTKYILGSLDTKGEYQPNFIDLQAYLTYDFHPDWQLAMIGNFNS
                     ADYNFTPTETEVVTGGFFFPHKFSADFEGGERDRFRNNMAGLALNYVPQRRKNPSFVK
                     FLTSVYNGDEIEAYDILSFFKVSKLELDLKGGKPKEIGVLGDGIQHDFARNRLQNIIV
                     NGEIKAGIEIPRFNDEKGQESSHFLYGGVKIQQEHFRDRINEWSRTDSAGYSLPNSEE
                     GYFLDGVLKSVNDIVSQRMTAFGQETYTFKKVGKYEVQATVGARAGYWSFNDEWIFSP
                     RAQFLYRQLGKENLLTYKLAGGIYQQQPFYREMRRPDGTLNQNIRSQKSAQVIGGITY
                     EFTVGRKRPVRFKMITEAYYKDLWDQISYNVDNVRVRYSGENDSRAYARGVDFRINGE
                     FVNGFESWLNLSYLDTKEKINGVAHQDAFGNSVAWVPRPTSQYFSASLYFQDYLPGNK
                     DFRTHLQLNFGTGLPYGPPGENLIKRNALTFKPYQRVDIGFSYQMWDVSKREEHPQHF
                     LRWTQRTFISLEVFNLMDVQNVAAVNWVKDFNNINYYFPINLTSRRINIKLRFDL"
     gene            complement(4009..6942)
                     /locus_tag="PKV31_01865"
     CDS             complement(4009..6942)
                     /locus_tag="PKV31_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB91627.1"
                     /translation="MRNLPVFLSLICLFSINNLSGQSQIFKELQQREKAGTIVQKKIV
                     KNLLSEEGAEQVGLSKDGNAIYFNIDQPVIAEIIVHRPALLRVKIPSVSSGEELTLLL
                     FNKKPFAEGAVVTTSAGDRHPMSGGAYYRGTVEGDEHSVVALSFVDGEMVGVVSVNNR
                     QFDIGKVEHSDVHVIYDATKVNRKAPTGCEVKYVEGYKPDMIGEAQIETRAADKCVNI
                     YFEVSYSIYQNKGSVSAVENYVNGFFNVVSTLYGNENIQVVISEIFVWTSSDPFPTND
                     AGDALDYFTGYRTSFNGDIAQLLAYTDNGNGGIAWIDVLCNFGGAYSYSYSDIDMTYN
                     MLPNYSWTTNVVTHELGHNLGSEHTHWCGWQGGAIDNCYTTEGGCPPGPPPSNGGTLM
                     SYCHLTGYGINYANGFGPQPGDLLRSKIAQAACLGTCGPQCPTFTIDGAVADVSCFGA
                     ADGSIVLIEPNNGTSPFSYTWSNGATTKDVYNLPKGTYSVSITDAAGCPGEASFAITE
                     PAKITLQVVKEDVSCHGLTDGSLIALANGGASGFSYQWSNGATTDMIGNLSAGTYTVT
                     VTDITNCTTQASATITEPAALSLTASITGVSCNGEDNGSITVMVSGGTFPYLFHWNNG
                     SNASSIANLSAGSYTVTVTDSKLCESIATYTITEPPLLNVHVATTDASSDVAADGTAE
                     AIAEGGKAPYSYLWSTGATTPKITGLTAGNYSVTVTDANGCKKVSTITIQGKECLLTV
                     VASGTSVTCAGLEDGSATAIPANNTGNAEYLWSNGATTQTISGLAPGGYSVIVTDNIG
                     CTATTTITITQPDTITLKIISITPSTTSTSQNGSISIEVTGGTPGYTFIWYSSGKEVS
                     TEQNPSNLNSGFYHVVIKDSHGCIHTSPEIFVDFLLSSNSTKQDPVRIFPNPANGFIY
                     ISYIDEYPGDISMYDILGRKVTLSITKEQNNVRLETSSLADGPYIIQIRMGRSVYAAR
                     VVVHH"
     gene            7410..8090
                     /locus_tag="PKV31_01870"
     CDS             7410..8090
                     /locus_tag="PKV31_01870"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNB91628.1"
                     /translation="METLFELFANLIDPDWIMSQGGLYLVLVILFIETGLFFGFFLPG
                     DVLLFISGIILASADQTVHIFDHKVIDLVFWQSLFIGSTILGNFVGYWFGSKFGHALF
                     YQGRKFLFLRQKHIDAAQQFYEKRGGFAVAIARFLPIARTFVPIVAGMVKMNFKQFTF
                     YNVLGAFLWVGGLTTLGFVLGKREWVADNLEWAVLAVLVLASLPMIIKMIFPGKKQGA
                     KKEKIRIL"
     gene            8102..9049
                     /locus_tag="PKV31_01875"
     CDS             8102..9049
                     /locus_tag="PKV31_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNB91629.1"
                     /translation="MLWIIFITFIIIALALDLGVFNKTPHIIKTKEATAWTLVWISLA
                     LLFSGIIYLAFEHQWVENPTSLTPYNAVLKYISGYLIEISLSVDNIFIIAVIFSSFSI
                     PQKYQHEVLFYGVLGAIVFRAIMIFFGVALITHFSWIIYVFGAFLIITAYRMLTHKSE
                     DFNPKKSKLFSLIKKIYPVTYMMDGDKFFIKKRGIKAATPLFVALVIIELTDLFFAID
                     SIPAILAISADPFIVFSSNILAVMGLRSMYFLISGMLNKFRYINYSLVVILAFVGVKM
                     ILSHHVEFPEWVSLGVIVASLTGGIMASLLIPEKKELSA"
     gene            9042..9677
                     /locus_tag="PKV31_01880"
     CDS             9042..9677
                     /locus_tag="PKV31_01880"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNB91630.1"
                     /translation="MRRNIALVLLGLLSFFSSNAQFIDDWEARPSVSIAYQVNKKLEV
                     EATYYLYLIDNLSRFEKSLFSGELEYEFAPWAVAGLDYRYGTGSEVDYHDLRYSIEFA
                     HPWKSGKWILVYRPTFQQKIKIGVDTDNYLRNKLELNFLLNPKVNLFIFTENYQLIDR
                     GLSFNTQKNAIGGIYSINRRHKLELQFTLKNKRDHKNYARLETAYVFKFGQ"
     gene            complement(9932..12154)
                     /locus_tag="PKV31_01885"
     CDS             complement(9932..12154)
                     /locus_tag="PKV31_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB91631.1"
                     /translation="MNVKYLWIAVILTLSNLMYAQNEDGIRSKLIDQHGTPIIAAWVS
                     INHSATKVQTDENGYFLLKTSSWPAHLDIDMGDNNVVHVDIANEEEAKVITAKKVVEL
                     HEVVVKEKVTRELNTIQTRNVETITRKEFQKAACCRLSESFDNTGSVSVSETDAVTGA
                     KEMEILGLRGIYSLMTLDNIPDFCGINFPFALDMIPGTWLDEVSISKGISNAINGSYG
                     FSGQVNVGLKNPFEDKPLFVNLYGNTMGRYEANVHMNKVLPNPAFATGLYLHASKNFS
                     KIDHNHDNYLDMPISTQLNALYKLKINGLGPWESNLDVQFVRDDRKSGQKTVSAEHPY
                     TATSDAQRFTINGNTGFVGFDKEGRSIGFKYQYTNNILDAAYGGLNYAGTQNRGYFQA
                     LYEDRFDDARHIIYAGASILAEGLKQKIAGTDFNRNELVPGVYAEYAYNPEITDVNKK
                     FTERFGLVAAVRADFHNIYGTQIAPALTAKYNITPDMVLRANVGKGYRTPHFFTDNIS
                     SFVNGRPIQITENLDPEEALNYGLSYTLKSKIARRNFSINADLYQTRFQNQIIVDQET
                     DLSAVRVSNLNGESVTTSALLSTSYEILNDFTAKIAYKWNDIRYDQAGQQVTKILIPR
                     HRALLALHYTTPDKGWEFSNTTTWIGPQEYLERIQVEGGVAQIRHEAKSFVTINAQIT
                     KKWNDFDLYFGGENLTNYTQKNPIQGGDAPQSDLFDVSQVYAPLAGIRGYVGIRWSIL
                     "
     gene            complement(12234..12710)
                     /locus_tag="PKV31_01890"
     CDS             complement(12234..12710)
                     /locus_tag="PKV31_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91632.1"
                     /translation="MKEFFKNTGLYSLVFLLYLSNTGLILTVHTCIHTGISVVELPSF
                     QTHDDVCSEVIGERSCAQDDNADDCCSREIEKKEDCHTHKTEYKKADYTSIKPEPNKY
                     GTFTGVAEVSFIYYQILPLIPKEREEHHIIYHANKARDNTTESALEYRIGLASFLC"
     assembly_gap    13079..13178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13439..14743
                     /locus_tag="PKV31_01895"
     CDS             13439..14743
                     /locus_tag="PKV31_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNB91633.1"
                     /translation="MRLLKYFPVFLLLLSCNPVGKQENPQSDFIRTEGIHFMQGDSTY
                     YFFGTNYWYGPALAMLEDTTRGIDRLRKELDFLKSHGVVNLRICAAVEGSGIIQGVPR
                     IFPVLQTAPGVFETANLRGLDRLLDEMKKREMRAVLFFSNNWEWSGGFLQYLYWNKMV
                     EEKDFVERMPWEQLRDVVSRFYSCEPCMDQYSAQVRTVLARTNSINGVKYTEDPTIFA
                     WQLANEPRPMRPAAIDAYKAWITRTAAMIKSLDSNHLVTIGVEGEIGTENMEVFKAIH
                     SDKNIDYGTIHIWPRNWAWYKDLNDEEQQAGSMQLTDDYIRKHIDAMREIGKPLVIEE
                     YGYPRDNNSFEPTSATTRRDGFYEKILLHWQESFAQKGVLAGCNFWAFGGTARPIDGQ
                     KFWKPGDNFMGDPPMEEQGLYSVFDSDTSTWKVIEKYIGRKQ"
     gene            14748..>15120
                     /locus_tag="PKV31_01900"
     CDS             14748..>15120
                     /locus_tag="PKV31_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNB91634.1"
                     /translation="MKIKNFEFKAKVSCIDEYESKLLAKGAVFIGTDHQIDTYFNTSR
                     GRLKLREGNIENALIHYEREDTASIKESRIILYKSKPDPALRDILILHMGVRVVVDKI
                     RKIYFIDNVKFHFDEVRGLGSF"
BASE COUNT         4180 a         3347 c         3290 g         4203 t
ORIGIN      
        1 gggaaaatcg acaatcttca ggtctatccc aatccggtga ataatgtcgt gaatattgca
       61 ggcgtcgccc ctaacagtgt catcaaaatt tttgatatgg caggaaatca ggttaaccga
      121 aaattcactt tatcagctat gaattctttt gacttatccg cctgtaaaga aggtatttac
      181 ttaatagtgg tgagcgatgg ttcaggaaat actgaaacat tcaaaatatt gaaaaacaag
      241 gttccttaac cgaaatttta aatactacga ctaatctgcc cggcttatcg aaaagtagcc
      301 gggctttttt tgatttttca agagtccctg attaaggatc ccagtaagac aaaagactat
      361 gattatttaa agggctacat catgtgtggt ttttccatgt ccggcattta tcttatcttc
      421 acccttctaa accgtacaca atgagcggac ttatagctcc ttcgatactc tctgccgact
      481 ttaccaaact ggctgacgaa atctctatga tcaaccgaag tgaagccgat tggtttcata
      541 tcgatgttat ggacggagtt tttgtaccca atatcaccat gggctttctc gtcgtcaagg
      601 caatcaacca actggctgaa aagcccatgg atgtgcattt gatgacagtg cacccggaat
      661 tgtacatcga acaatggcgc gatgcaggag cggatcacat caccgtacac tacgaagcct
      721 gcacacatct gcatcgtgtg attcagcaga tcaaacatac cggagcaaag gcaggagtcg
      781 ccatcaatcc gcacacgccg gtcaaactat tggaggacat catcgaagat ctggatcttg
      841 tcatcgtcat gtcggtcaat cccggatttg gtggccaggc cttcattcca cgcacctttg
      901 acaaggtccg ggcactttca gccatgatca gggaacgaaa tgcatccacc cttatagaga
      961 ttgatggagg tgtcaccctt gccaacgccg gtgcactgat tgaatcaggc gctcatgctc
     1021 tcgtggcagg caataccgtc ttcaactctc ccaatcctac ggagacgatt catttattaa
     1081 aaaaaacctg ctctcatact aaacataacg catgatgtcg ttagacagag agcttaatcc
     1141 aatccgaatg ccattgttta tacgtctctt ttttcctgtt ttcttcctct tgctttgttt
     1201 caagttagat gctcaggaaa caagtactct tgttttggga aaaataattg atgaaataag
     1261 cggggagcct gtcgaagccg ccttgattta tgaaaagagc gaacaaatcg gcgtagagag
     1321 tgatgtaaat ggcttttttc aactatcggt gcctgcggga aaacctttaa ccatcatcat
     1381 tcggagggtt gggtatgaga cagcggccat caaacttaat tcaattaccg aaggaaccat
     1441 taaaaaactt gatattagtc ttatccagaa agacgcagga cagggggtta ccatatatgc
     1501 tgaatccatt ggagacaaaa atatggttcg tgccaatgtg aaagaattca agctgctccc
     1561 caccgtttcc ggcaatctgg agagcgtcct gccttccatt gccctgggca cctcctccgg
     1621 ctcaggaggt gaattgactt cccaatacaa tgttcgcggt ggcaattatg acgaaaacct
     1681 ggtctatgtc aatgactttg aaatattccg tcctcagttg attcgctccg gccaacagga
     1741 gggcctttcc ttcccgaata ttgatctgat acgcgatctc gccttcagca gtggtggctt
     1801 cgaggccaaa tacggggata agatgtcctc tgtcctcgat atcaaataca aaagacctga
     1861 agagcgccgt ggctcggttt ccggcagcct gctgggagca acagcacacc tggagggatt
     1921 gatgaagcca tttaaaaaca ggggcggaca attgagatat cttttgggag caaggtacaa
     1981 aaccaccaaa tatattcttg gttccctgga tactaaagga gaatatcaac ccaacttcat
     2041 cgacctgcag gcctatttga cctatgactt ccatcccgac tggcagctag ccatgatcgg
     2101 caactttaac tcagccgatt ataatttcac tccaacggaa accgaagtag tgacaggtgg
     2161 ttttttcttc ccacataagt tcagcgcgga tttcgaaggt ggagaacggg atcggtttag
     2221 gaacaatatg gcagggcttg cactaaatta cgtcccacaa agaagaaaaa atccatcttt
     2281 tgtcaagttc ctgacgtctg tctataacgg tgatgaaata gaggcatacg atattctttc
     2341 ttttttcaag gtttccaagc tggaacttga cctcaaggga ggcaaaccca aggaaatcgg
     2401 tgtgctgggt gatggtatcc aacatgattt cgccagaaac agattacaga acatcatcgt
     2461 caacggtgag ataaaggcgg gaattgaaat cccgagattt aatgacgaaa aaggtcaaga
     2521 atcttcacat tttctttatg gtggtgtcaa gatccagcag gaacacttcc gtgaccgaat
     2581 caatgaatgg tccaggacgg acagtgccgg atactccctg cccaactccg aagaaggcta
     2641 cttcctggat ggcgtcctca aatcggtcaa tgatatcgta tcacaacgta tgaccgcttt
     2701 cgggcaggag acctatacct tcaagaaagt aggcaaatac gaagtacagg caaccgtcgg
     2761 agcccgcgca ggatactgga gtttcaacga cgaatggata ttctcaccca gagcgcaatt
     2821 cctgtacaga cagcttggaa aggaaaacct actgacctat aaactggccg gaggaatcta
     2881 tcaacaacag ccattctacc gggaaatgcg ccgaccggat ggtacactta accaaaatat
     2941 ccgctcccag aaatctgctc aggtcatcgg cggtattact tatgaattta ctgtcggcag
     3001 aaaacgtcct gtgcggttca agatgattac agaagcctat tacaaggact tgtgggatca
     3061 gatatcctac aatgtagaca atgtgcgcgt taggtacagt ggtgaaaatg acagccgcgc
     3121 ttatgctcgt ggggttgatt tcaggatcaa tggcgaattt gtaaatggct ttgaaagctg
     3181 gctcaatttg tcctaccttg atacaaaaga aaaaataaat ggcgtagctc atcaggatgc
     3241 cttcggcaat tccgtggcat gggtgccccg accgacatca caatatttct cggcatcact
     3301 ttattttcag gattatcttc cgggcaacaa agatttccgg acacaccttc agcttaattt
     3361 cggcaccggc ctcccatacg gacctccggg agagaatctg atcaagagaa atgcccttac
     3421 attcaaacca taccagcgcg tagatattgg cttttcctat cagatgtggg acgtatcaaa
     3481 aagagaagaa catccacaac acttcttacg atggacgcaa agaacattca tcagtctgga
     3541 agtatttaac cttatggacg tacagaatgt tgctgcagtc aactgggtca aggattttaa
     3601 caacattaat tattattttc caatcaacct gaccagccga aggataaata tcaagctgag
     3661 gtttgacctc tgacagtctg actttggcac aatatcatcg aacaatcctc ataccttggc
     3721 gattgataat atcctcccct cgggaaaaag tataagcagc cacaagcgcc atctgcttga
     3781 tctgcatcag taaacatgag cacgagtcca attatccgat gactggtacc cgtcatttcg
     3841 atgtagtcca ttcataggca tgacgttaaa gggaaatctc caccttttga aaggcaaaaa
     3901 atggtcgatt catgtgagac aggtaccgaa tcatttgtga attcagcaac tccttccttg
     3961 tctatgaatt cagctcatcg cgggaagtgg gactgatgtc caacaacatc aatgatgcac
     4021 caccactctg gctgcatata cactccgacc cattctaatc tgaatgatat atggcccgtc
     4081 cgctaaggac gatgtttcaa gccggacatt attttgttcc ttcgttatgc ttaaagtaac
     4141 tttccggcca agaatatcat acattgatat atcgcctgga tactcatcaa tataagatat
     4201 atagataaag ccatttgccg gattcgggaa gattctgaca ggatcttgct tggtcgaatt
     4261 ggaactaagg aggaaatcca caaagatttc cggactcgta tggatacatc catggctatc
     4321 cttgattacg acatggtaaa agccggaatt gagattggat ggattctgct cggtagaaac
     4381 ctccttgccg gaagaatacc aaataaatgt atatcccgga gttccaccgg tcacttcgat
     4441 ggagatgctg ccattctgtg acgtacttgt cgtggacggc gttattgata tgattttcaa
     4501 ggtgatggtg tccggttggg taatggttat tgtagtagtg gccgtacaac ctatattatc
     4561 ggtgacgatt accgagtatc caccgggtgc caggcctgat attgtctgag tggtagcgcc
     4621 attggaccac aggtattcag catttccggt attgttggcg ggaatggccg tggcggagcc
     4681 gtcttcaagt cccgcacaag taacagatgt gccggacgca actactgtaa gcagacattc
     4741 cttcccctgt atcgtgatag tagatacttt tttacaacca ttggcgtcgg tgacggtcac
     4801 agaataattg cctgcagtga gtccggtaat ttttggcgtt gtagctccgg tagaccacag
     4861 ataactatat ggcgctttgc ccccttctgc tattgcctcc gcagttccat cagctgccac
     4921 atcggatgag gcatccgtgg ttgcgacatg gacgttgagc agaggcggct ctgtaattgt
     4981 gtaggtagct atgctctcac atagtttcga atcagtcaca gtgactgtgt acgatcctgc
     5041 actgaggttg gcaatggaag atgcattgct gccgttattc caatggaaca gatacggaaa
     5101 agtgccccct gaaaccatca ctgtaatcga accattgtct tctccattgc agcttactcc
     5161 tgttatgctt gccgtaaggc tgagcgcggc cggctctgtt atggtcgctg atgcttgtgt
     5221 cgtacagttg gtgatatctg tcaccgtgac agtgtaggtt cctgcagaca gattgccgat
     5281 catgtcagtt gttgcgccat tactccactg atagctgaag cctgatgcac caccatttgc
     5341 gagagcaatc aaagaaccgt cagtcaggcc atggcaggac acatcctcct taactacctg
     5401 gagagttatc tttgccggct cagtaatagc aaatgatgcc tcaccgggac agccagccgc
     5461 atcggtgatc gacacgctgt aagtgccttt gggaagattg taaacatcct ttgttgtagc
     5521 gccgttagac catgtatagg agaatggaga tgttccgtta tttggctcga taaggacgat
     5581 gctgccatcg gctgccccaa agcaggacac atcggcaacg gcgccatcta ttgtgaaagt
     5641 gggacattgt ggcccgcatg ttcccaggca ggcagcttgg gcgattttac tccgcaacag
     5701 atctcccggc tgagggccga aaccattggc atagttgatc ccatatcctg tcaaatgaca
     5761 atagctcatc aatgtgccac cattggacgg aggtggaccg ggaggacaac caccttcagt
     5821 agtataacag ttgtcgattg ccccaccttg ccaaccacac caatgtgtat gttcggaacc
     5881 caggttgtgc cctagctcat gagtcaccac attggtagtc caggagtaat tcggcaacat
     5941 attataggtc atgtcaatat ccgaataact gtacgaatac gctccaccaa aattgcataa
     6001 tacatctatc caggcaatcc ctccattgcc attatcggta tatgccagta actgagcaat
     6061 gtcaccattg aaggatgttc tgtaaccggt aaaataatcc agggcatcac cagcatcatt
     6121 agtgggaaat ggatccgatg atgtccagac gaaaatttcg gatatcacaa cctggatatt
     6181 ttcattacca tataaggtcg agacgacgtt gaagaagcca ttgacatagt tctcaacagc
     6241 actgacactg cctttattct gataaataga atacgatacc tcaaagtaaa tattgacaca
     6301 cttgtcggca gccctggttt caatctgagc ctctccaatc atgtcgggct tgtatccctc
     6361 gacatatttt acctcacaac cggtgggcgc cttcctgttg accttagtgg catcatagat
     6421 gacatgtaca tctgaatgtt caaccttgcc gatatcaaac tggcgattat tgactgaaac
     6481 gacgcccacc atctccccat ccacaaaact taatgccacc acactgtgct catcgccttc
     6541 aactgttcca cggtaataag ctcctccgga catgggatgt ctgtctccgg cactcgtagt
     6601 aacgacagca ccttcagcga aaggcttttt gttgaacaag agcagggtga gttcctcccc
     6661 cgatgaaacc gatggtatct ttacccttag gagggcaggc cgatgtacga ttatttcagc
     6721 gattacaggc tgatctatat tgaagtaaat ggcgttaccg tccttactta atcctacttg
     6781 ttctgcacct tcctcagaaa gcaggttttt gacaatcttc ttttggacaa tagtccctgc
     6841 cttctctctt tgttgcagtt ctttaaatat ctggctctga ccggaaagat tgttaataga
     6901 aaaaaggcaa atgagtgaaa gaaagacagg taagtttctc atataatcaa tagaagtatt
     6961 aaataattca catatattga aagaacaaag gtagtaaaaa gatgtgcact taaagtaatt
     7021 caatcaattc cgtgcaagta gatatattta gtttttatag cgtctcttcg cagctaaatt
     7081 tccggatgtg acgggacagg gtcccgggag tcggatcaac cagcccgggc aacctgagcc
     7141 atcttcatcc ttcaagaaat cccaataacc acaaaaaacc gaggagacta tttatctatg
     7201 tcacagcact ctggtgtcat tacattttca aagtcatcag cattttattt cagtcaacta
     7261 accacacttt ataaattttg aatgaaagga accgatatta ataaatagaa taaaaacaaa
     7321 tcaatcaagt tttgatgttt ttaaaaacat taaccaatta aagtgaaccg gttgataaat
     7381 aatctgttac tttgtcaaaa ttataaattg tggaaacact gtttgaatta tttgccaatt
     7441 tgattgatcc ggattggatc atgagccagg gcggactgta ccttgttctt gtgattcttt
     7501 ttatagaaac aggccttttc ttcggatttt ttcttcccgg cgatgtcctg ctgtttattt
     7561 ccggaattat attggcaagt gccgaccaga cagttcatat ttttgaccat aaagtgatcg
     7621 acctcgtctt ttggcaatca ttatttatcg gaagtacaat tcttggtaac tttgttggat
     7681 attggtttgg aagcaaattt ggccatgctc tgttctatca aggcaggaaa ttccttttcc
     7741 tcaggcaaaa acatattgat gccgctcagc aattctatga gaaacgaggg ggttttgcag
     7801 ttgcaattgc gcgttttttg cccattgcca ggacttttgt ccccatcgtt gcaggtatgg
     7861 taaaaatgaa tttcaaacag tttacattct ataatgtgct tggcgcattc ctttgggtcg
     7921 gaggcctaac cacgcttgga tttgtactcg gcaaaaggga atgggtggct gacaatttgg
     7981 agtgggcggt cttggccgtt ttagtcctgg cctctttacc gatgatcatc aagatgattt
     8041 ttcccggtaa aaaacaaggc gccaaaaaag aaaaaatacg aattctataa taagctaggt
     8101 tatgttgtgg ataattttta ttacttttat cataatagca ttggcgctgg acctgggtgt
     8161 attcaacaag acgccccaca tcatcaaaac taaggaggcg actgcatgga ctcttgtatg
     8221 gataagtttg gcactgctgt tttccggaat catatatctc gcttttgaac atcaatgggt
     8281 tgaaaatccc accagcctaa ctccatataa tgcagttttg aagtatatct caggctacct
     8341 gatcgaaata tcgcttagcg tagacaatat tttcatcatt gccgtcatat tctcatcctt
     8401 ttcaatacca caaaaatacc agcacgaagt tttgttctac ggtgttttgg gcgcaatagt
     8461 tttccgggcc atcatgatct ttttcggggt tgccctgatt acccatttca gctggattat
     8521 ttatgtgttt ggagccttcc tgatcatcac ggcatacagg atgcttacac ataaaagcga
     8581 ggatttcaat cctaaaaagt caaaattatt ctcgttgatt aaaaaaatat acccggtaac
     8641 ctacatgatg gacggggaca aattttttat taaaaagagg gggataaaag ccgcgacacc
     8701 gcttttcgta gcattggtca tcatcgaact caccgatttg ttttttgcta tcgacagcat
     8761 tcctgcaatt ctagccattt ccgcagatcc tttcatcgtt ttcagctcga atattttggc
     8821 cgttatgggc cttcgatcca tgtatttcct gatatccggc atgttgaata aattcagata
     8881 tatcaactat agtctggttg ttattcttgc cttcgtaggt gtaaagatga tcctgtcaca
     8941 tcatgtggaa ttcccggaat gggtctccct gggagttatt gtagcttccc tgaccggagg
     9001 tattatggca tcgctcttga ttcccgaaaa aaaggaattg agtgcgtaga aatatcgctc
     9061 ttgtcctgtt gggcttactt tccttttttt catccaatgc ccagtttatt gatgactggg
     9121 aagcacggcc ttctgtctcg atagcctatc aggtcaataa gaagctggaa gtggaagcta
     9181 cttattattt gtatttgata gacaatcttt cacgattcga aaaatcctta tttagtggag
     9241 agttggaata cgagtttgct ccatgggctg tagcagggtt ggactatcgt tatggtacag
     9301 gttctgaagt cgattatcac gacctgcgat attccataga atttgcacat ccatggaaat
     9361 caggaaaatg gattttggtg tatcggccta cctttcaaca aaaaataaaa atcggcgtcg
     9421 atacagacaa ttacctgagg aataagctcg aactaaactt tctgcttaat ccaaaggtca
     9481 atctgtttat ttttactgaa aactatcagt tgattgaccg tggcttatct ttcaataccc
     9541 agaaaaatgc aatcggcggt atttattcaa tcaaccggag gcacaaatta gaactgcaat
     9601 ttaccttaaa aaacaaaaga gaccataaga attatgcaag gcttgagact gcgtatgtct
     9661 ttaagttcgg gcaataaaat aatagattct aatccctcac aaaattacct tctgctagtt
     9721 aataatgatc tgatggggat gaagagcatt aacgagaagg catctttacc catcctacac
     9781 aagaaagtca cgtcacggat gatgtaatat ttagccagaa tacctgtcga tccaagcact
     9841 tcatgatcca gtcaaaaagc aaaaaaggca caaatcacct gtttgcaatt tgtgcctttt
     9901 agcagcagga acaacttacc tacccactac attacagaat actccagcga atgcctacat
     9961 aacctctgat accggcaagt ggtgcatata cctggcttac gtcaaacaag tctgactgcg
    10021 gagcgtctcc tccctgtatc ggattctttt gggtgtaatt ggtcaggttt tctccaccaa
    10081 aatacaagtc aaaatcattc cacttctttg ttatctgagc attaatggtc acgaaagatt
    10141 tcgcctcatg tcggatctgt gccacgcctc cttccacttg tattctttcc agatattcct
    10201 gtgggccgat ccatgtcgtg gtgttgctaa actcccaacc cttatccggt gtagtatagt
    10261 gcaatgccag taaggcgcgg tgcctgggaa taaggatttt tgtcacttgt tgtccagcct
    10321 ggtcatatct tatgtcattc catttgtacg cgatcttggc ggtaaaatca ttcaaaatct
    10381 cgtaactcgt actcaacaga gcagaagtcg tgaccgactc tccgttgaga ttagagactc
    10441 gtaccgcaga aagatcagtt tcctgatcca cgatgatttg attctggaat cgtgtctgat
    10501 aaagatcggc attgatggag aaattcctgc gcgcaatctt cgactttagt gtataggaca
    10561 gaccatagtt aagtgcttct tcgggatcga ggttttcagt aatctgtata ggccttccat
    10621 tgacaaagga ggaaatgttg tctgtaaaga agtgcggagt gcgatagcct ttgcctacat
    10681 tggcacgcaa taccatatcc ggtgtaatgt tgtatttggc ggtaagtgcg ggagcaatct
    10741 gtgttccgta gatattatga aaatccgctc tgactgctgc tacaagtcca aatcgttccg
    10801 taaatttttt attgacatcg gtaatttccg ggttgtatgc atattcggca tacactcctg
    10861 gcaccaattc gtttctgttg aaatcggtac cggctatttt ctgtttaaga ccttctgcaa
    10921 gaatgcttgc acccgcatat atgatatgcc ttgcatcatc aaaccggtct tcgtacaatg
    10981 cctggaagta gccgcggttt tgagtgccgg catagtttag acctccatag gctgcgtcca
    11041 ggatattgtt ggtatattga tacttgaatc ctatgctcct accctccttg tcgaatccaa
    11101 caaaacccgt gtttccattg atggtaaatc gctgggcgtc acttgtggcc gtataaggat
    11161 gctcagcact gacagttttc tgtccggact ttctgtcgtc cctgacaaac tgtacatcca
    11221 agttgctttc ccagggaccc agtccgttta tttttagttt gtacaaagca ttcagctgag
    11281 tcgatatggg catgtccagg taattgtcgt gattgtgatc aatctttgaa aaattcttgc
    11341 tggcatgcag gtacagtcct gtggcgaatg ccggattggg aagcactttg ttcatatgga
    11401 cattggcttc atagcgaccc attgtattgc catacaagtt gacaaagaga ggcttgtctt
    11461 caaaaggatt cttcaaacca acgttgacct gtccggaaaa tccataactg ccgttgattg
    11521 cgttggagat tcccttggag atggacacct catccaacca ggtaccgggt atcatgtcca
    11581 gggcaaatgg aaagttgatc ccgcagaaat caggaatgtt gtccaaagtc atcaggctgt
    11641 atatgccacg caatccgagt atctccatct ccttggcacc ggtcacggca tcggtttcac
    11701 ttacgctgac tgaacccgta ttgtcaaagc tctcactgag tcgacaacat gctgcctttt
    11761 gaaattcttt tctggtgatg gtttcgacat ttctggtttg gatggtattc aattcgcggg
    11821 taactttttc cttaaccact acttcgtgca gttcgaccac tttcttagca gtgatgacct
    11881 ttgcttcttc ttcattggcg atatcgacat gcactacatt gttatccccc atgtcgatat
    11941 ccagatgtgc cggccaggaa gatgttttca acaggaagta accgttttcg tcagtctgaa
    12001 ccttagtggc actatgattg atgcttaccc aagcggcaat aatgggagtt ccgtgttggt
    12061 cgatcagctt ggagcgaatt ccgtcttcat tctgggcata cataagattg gaaagtgtca
    12121 ggataacagc tatccataaa tattttacat tcattttttg atattattgg ttgagtatat
    12181 ggcaagagcc gaagcccgtg caatcactaa ctgggtgaag aaccaacaat atgctaacaa
    12241 aggaaggagg cgagccctat ccggtattcg agggccgact cagtagtatt atctctggct
    12301 ttatttgcgt gatagatgat atggtgttcc tctctttctt ttggaatcaa agggagaatc
    12361 tggtaataaa taaaagaaac ttcggctaca ccggtaaaag tcccatattt attgggttcc
    12421 ggtttgatgg aggtgtagtc tgctttttta tactccgtct tatgtgtatg acaatcttcc
    12481 tttttctcta tttccctgga acagcagtca tccgcattgt catcctgagc acaagacctc
    12541 tcaccgatga cttctgaaca cacatcatca tgagtctgaa atgaaggtaa ttcgacaacg
    12601 gaaataccgg tatgaatgca tgtatgcact gtaagtatca atcccgtgtt gctcagatac
    12661 agcaaaaaca caagagaata taagccggta tttttgaaaa attccttcaa gttatagctt
    12721 ttcgaccgca aaggtagtaa atgcctcatt ctcttattgt taaaaattta ttaatgtttg
    12781 agcgtttacc ggttaagcgc tatgtcattg gataagcgaa agcaaataat taccggcgta
    12841 tccaccacct ttgcctgaaa tcataaagac tttctcactg catgtacaac gcttccacat
    12901 cctgcatgac gttccgacgg aacaggaaga tattcacttc atttcaaatc aaacaatgaa
    12961 tttctcttta ataaaccaat tgtctttgca taacggatgt gtctttgatc caaaaatggt
    13021 aatttatttt ttttaacagg aaatacactg ccggagcaca tggccgtttt gtttttttnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ctcaatgttt gtcttatcac
    13201 tgaaaatttg agctgcctcg aaaattttca agctatgcat tgagataaat caatacctga
    13261 taacaaagat cttgccaggt taaagtgagg taaagaatta tttggagggt aatgtcatgc
    13321 attgaatccg ggaaatattt tcaataatca aaaatacagt ccgataaata ccggcagcct
    13381 attgcccacg ccaaatattt tgacaaactt tggcatttga aatttaatct gtcagcacat
    13441 gagactcctt aaatacttcc ccgttttcct tttgttattg tcctgcaatc cggtcggaaa
    13501 gcaggaaaac cctcaatccg acttcataag aacagaaggc attcacttca tgcaagggga
    13561 ttccacctat tattttttcg gcacgaatta ttggtatggg cctgctttag ccatgcttga
    13621 agatacaaca agaggaatcg acaggctacg aaaagagctc gactttctca agtcacatgg
    13681 agtagtcaat ctccgtattt gtgctgctgt ggagggtagt ggcatcatcc agggcgtacc
    13741 gcggatattt ccggtactgc agaccgctcc gggtgtgttt gaaaccgcca atctccgggg
    13801 cctcgatagg ctgctcgacg aaatgaagaa aagggagatg cgggcagtac tgtttttcag
    13861 caacaactgg gagtggagtg gtggctttct acagtatctt tattggaata aaatggttga
    13921 ggaaaaggac tttgtggaaa ggatgccctg ggaacaactg cgcgatgttg tcagtcgatt
    13981 ttattcttgt gagccgtgta tggaccaata ttcggcacaa gtacgaaccg tcctggctcg
    14041 taccaacagt atcaatggcg taaaatatac ggaagatcca acgatctttg cctggcaact
    14101 tgccaacgaa ccacgtccga tgcgccctgc agccattgac gcctacaagg catggatcac
    14161 ccgtactgct gccatgatca aatcactgga tagcaaccac ctggttacta taggtgtgga
    14221 gggtgagatc ggaaccgaaa acatggaagt tttcaaggcc atccattccg acaagaacat
    14281 cgattatggc acgatccata tctggcctcg caactgggcc tggtacaagg atctgaacga
    14341 cgaagagcaa caagccggat ccatgcagct taccgatgat tatatccgaa agcacataga
    14401 tgccatgagg gagataggga agccccttgt aatagaagaa tatggctacc caagggataa
    14461 caacagtttt gaacccactt ccgccacaac aagacgagac ggattctatg agaaaatatt
    14521 gctgcattgg caagaatcct ttgcgcaaaa aggcgttctg gcaggatgta atttctgggc
    14581 tttcggaggc accgccaggc ctatcgatgg tcagaaattc tggaagccgg gcgataattt
    14641 tatgggcgac ccaccgatgg aggagcaagg cctctattcg gtatttgaca gcgatacatc
    14701 aacctggaag gtaatcgaaa aatatatcgg caggaaacaa taaccttatg aagatcaaga
    14761 actttgagtt caaggcaaaa gtttcgtgca ttgacgaata tgaatccaaa ttgttggcaa
    14821 agggggcagt gtttataggt acagatcacc agatcgatac ctatttcaat acgtctcgcg
    14881 gaaggctcaa gctaagagaa ggtaacatag aaaatgccct gatccactac gaaagggaag
    14941 ataccgcttc gatcaaggaa tcccggatca tcctgtacaa aagcaagccg gatccggcac
    15001 tcagggacat cctgatcctt catatgggcg tccgggtagt cgtggataaa atccgaaaaa
    15061 tctactttat tgataatgtc aaatttcatt ttgatgaggt cagaggactg ggctccttca
//
LOCUS       DAOQMC010000025        15059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1441_length_15059_cov_4.769262, whole genome shotgun sequence.
ACCESSION   DAOQMC010000025 DAOQMC010000000
VERSION     DAOQMC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15059
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1441_length_15059_cov_4.769262"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKV31_01905"
     CDS             complement(<1..389)
                     /locus_tag="PKV31_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNB91635.1"
                     /translation="MADRKIKVAAYQYTIVYYHSLDEWRERMEGIVREGCAEGQHLLL
                     FPEYGSMDLVSLFPKEIQQDIHTQLDEMQDLLSEFKGHFSDLARKYGVIIVAPSFPVK
                     ESAGLFNRIYVFSPSGKEGHQDKWWMTR"
     gene            complement(470..1069)
                     /locus_tag="PKV31_01910"
     CDS             complement(470..1069)
                     /locus_tag="PKV31_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB91636.1"
                     /translation="MMADGLTFVTKTGSEIAGLFDGLARLRIAVFYEFPYLYEGDFDY
                     ERDYLSIYTSSTKSLVHAVFDGDQMVGATTCMPLSDEVEEVKMPFVKAGMDVGKIFYF
                     GESILLPEYRRRGLGHRFFDVREAHALSFGTYTTTCFCAVNRPVDHPLRPENYRPNDA
                     FWTKRGYIKHPELIAEFEWLDRNMDLPDKKEMDFWLRNF"
     gene            complement(1066..1467)
                     /locus_tag="PKV31_01915"
     CDS             complement(1066..1467)
                     /locus_tag="PKV31_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNB91637.1"
                     /translation="MTSLEIVKNYYDCFNRKDWQGMLDLVDDAVVHFPNEGKPRYGKE
                     LFAHFLNKMDDAYEERLEELVFYTTSDEGKVSVQFMVHGKYKVSEEGLPPAHGQVYVL
                     PAAAFLTVRNGRIMSVTTYYNLNEWIEKVSE"
     gene            complement(2025..2831)
                     /locus_tag="PKV31_01920"
     CDS             complement(2025..2831)
                     /locus_tag="PKV31_01920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB91638.1"
                     /translation="MKGVKKAFKALRELIHNPWLLNNVLDDNTVWHRKVIQEFGLPDG
                     LSCISLEQISPGFHETLETFSFLGGGSLPTDIALLKALCRRFDKAKYFEIGTWRGESV
                     RNVSDVAEECFTLNLADDQMRRLGLSEDYIDAHGFFSKNRKNITQLLGDSTVFDFSGL
                     DKRFDVIFIDGNHHHEYIVSDTRNVFDHLIHEHSIVVWHDYAYDPDRVRFETLHAILE
                     AVPVGFHRYIHHVANTMCAIFIRDNFEVKALKPYEDPVHTYEVSIKSQKL"
     gene            complement(2818..4077)
                     /locus_tag="PKV31_01925"
     CDS             complement(2818..4077)
                     /locus_tag="PKV31_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB91639.1"
                     /translation="MSELFRFIREFIRNKGLMVFLSILVSRLSMLGINIIAARMITKD
                     EFGLISLVFSVFAIFAPLTGLGSYQGLLRYGVLETSQDAKDNLSRYVFGKGLFNHLII
                     ICIYIAISYIYTLRYGGIQIVILLFAVRLLGYYFQNFIESYFRIHHDNKMFAWIGMIT
                     GVGGFLMVLAGTWFWGMLGYLVALAVMPWLSFLFYRREAFSGIITMPSINLKEFWRYS
                     FHGGLTYFLSDILFSMDFMLIGFLLDGHAIAMYKTAIILPMSLAILPQIFMQTDYPKL
                     ALKYADKSYLEFYLKNYYKIFIPLGIIILIVGFLLRDRIIPFIFGEQYAGNGIVFFII
                     LAALVGNMWLRNLYGNLSSAIGKAQWNTYASLAAIVIILGLGVWLIPIMGIEGAAIGM
                     AAAFTFTGLMTMILFHKYLRNLGHERS"
     gene            complement(4074..5738)
                     /locus_tag="PKV31_01930"
     CDS             complement(4074..5738)
                     /locus_tag="PKV31_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNB91640.1"
                     /translation="MKSEFQISHLPKKELTFREMVDGVQSFLYHFFDNPSYRFFLKGV
                     VLNFNELIASYGTAADIEHLVTALYEKLGTGFSREFSGEYSGYLYDKRNGKVFVYTNL
                     TASQTIFYYHRDDFLLVDTGLASLKRSLDRHSLLYSIDMEASYCTLALNSLPDNFTLI
                     SEVRKLKGGEQLAFDVETRKMEIQSWEGPGSQDLYRGDKKSALHTLDELFTRAVRQEY
                     TFDLEHQVGHFALLSGGLDSRMGVLYSRRMGFRHEQALCFSHSGYWDEIIARQIAGKE
                     GLTLSVVHLDGGEFLKHIDKLVGITQGLACYTGGVHSEFALSTIDFADKGLIHTGHLG
                     DAVLGSYLSSPARNPASSAFPKLIANQRYADKVRPVLDRIAAKYPSEEAFLFYNRGFN
                     FIAVGNYVAEQFSVAVSPFMHNDFLRFALSLDEAWRYKEKIYIDWIRDFCPSAAGYIW
                     ERTLMKPDRTWKTAFGDQFKVRFRNLIYNKLLRQPYKCNMAPYEYYFRTNHDIALYFD
                     RYFSENIDLVHDPALKQDCLTMYLQGTFYEKTMVLSLLGSFKFLFG"
     gene            complement(5728..6864)
                     /locus_tag="PKV31_01935"
     CDS             complement(5728..6864)
                     /locus_tag="PKV31_01935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB91641.1"
                     /translation="MPKNRILFLASWYPSRVSPYSGDFIQRHARVAARIADITVLHTV
                     RDDDIAGAYEVSDTDNGLREIIVYYKDSGCKVLNFFKRMKAFWMGYIRAEVPDLVHLN
                     TVLPAGLFALYLKFFLGKPFILSEHWTGFQPFRFRQLNLMTHILVRLILQNCRLVLPV
                     SADLGKQMKAVCSKPEFEVVPNVVDTDRFRIFSERKSSAKPRFLHLSMLDEQHKNISG
                     ILRSAKRLVDSGLSFELHIGGNGPLEPIRQFVARHHLQDVIFPFGELSHDEVPAMMAN
                     ADCFLLFSNYENQPCVQGEAFACGLPFIGTDVGGIGEFLPETFGILIPKGDEDALYRA
                     MESVIRGRKFESPAEMHRFAEENFSPEHLANRFHDIYNRVMHEK"
     gene            complement(7580..8128)
                     /locus_tag="PKV31_01940"
     CDS             complement(7580..8128)
                     /locus_tag="PKV31_01940"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNB91642.1"
                     /translation="MKIFQYIIFFILAVLVYILYQYMYMKPAFMVGEKAPSFRAQTID
                     GKHFSLDDMSGKLVLLDFWGTWCSPCRKQLPELSKLYRTYRDSLHGIFEVVSVAMDDD
                     SAILRQVINSDSLAWPSHFREPKDGGPITTLYQIKKVPTSYLINDKGQIISVNPTIDD
                     LKSWLMKIDQAHYSKVRSQGVH"
     gene            8281..9027
                     /locus_tag="PKV31_01945"
     CDS             8281..9027
                     /locus_tag="PKV31_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNB91643.1"
                     /translation="MSTPDHFEYIDKSKLPRHVAIIMDGNGRWAKQQGKSRVLGHSAG
                     VKSVREVSEAAARLGIEYLTLYAFSTENWNRPKLEVEALMLLLVDTINKEIATLKKNN
                     IRLNAIGNLAGLPTASQKALKKGIDATKDNDGLNLILALNYSGREEITNMVKLISEEV
                     RAGSLEPGIIDNRIIEQHLYTSGMPQPELLIRTSGEYRISNFLLWQIAYTEFYFTPLM
                     WPEFGQQEFYKAIIEFQSRERRFGKISEQL"
     gene            9206..9706
                     /locus_tag="PKV31_01950"
     CDS             9206..9706
                     /locus_tag="PKV31_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91644.1"
                     /translation="MTKLTFIPLIIMLILSMNSGLAMIHSPDSTGPKVFLLGDDENLM
                     NSLSKNYPTLLMKVCKDDMEVAYNVWMEMLSDMEAYATKINFDIRGTKLWMNVYWNKN
                     GKIEYIGYYLKPNSRNVKTEEMNAFFASFMKNYQMNLSYTGRFYHNGSAAFPTHFKAL
                     KDKEGG"
     gene            9881..11263
                     /locus_tag="PKV31_01955"
     CDS             9881..11263
                     /locus_tag="PKV31_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HNB91645.1"
                     /translation="MGVKGRLTIISFMQFFVWGAWLITMANFWFGTKNWDGTQFGAVF
                     GTMGIASIFMPTLVGIIADRWINAERLYALLHILYGAVLFYLPNTTDSGAFFYTMLVA
                     MCFYMPTIALCNSISYNILKSNNYDVIKEFPPIRVWGTIGFIVAMWTTNLTGQKATEG
                     QFYIAGVAAILLGLYALTLPKCPPQKLIDKDAPLTHQLGLNAFRLFADYKMALFFIFS
                     MMLGAALQLTNAYGDVFLDEFKYFPKYASSFVIKYSTIIMSISQISETLFILAIPFFF
                     KRFGIKQVMLISMLAWVFRFGLFGFGDPAGGLWMIILSCIVYGMAFDFFNISGSLFVE
                     TTTEPKIRSSVQGLFMMMTNGFGAVLGSVIAGWSIDRFFTKKFIDIQSLAANFDTTAD
                     NPTFLDIAKNALGAVINQDGSLANPVYIKDWHQIWIAFAAYALLVTILFALLFKNEPK
                     QELDLSKLNH"
     gene            11466..12062
                     /gene="ruvA"
                     /locus_tag="PKV31_01960"
     CDS             11466..12062
                     /gene="ruvA"
                     /locus_tag="PKV31_01960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNB91646.1"
                     /translation="MIAYIHGKITGLTPTYALIETSGIGYHVNISLNTYAMLDGKETY
                     KLLTVLLIKEDSHTLYGFATEEERSVFTHLISVNGVGAGTARLILSSMPATDVKQAII
                     REDERMFSMVKGIGPKTAKRIIIDLKDKLSKEGDLPAGNIRLPLVSAIRDEAGAALLA
                     LGFQKNNIDKVLNTLLKEDKEYTLEQMVKAALKSLSGA"
     gene            12258..15023
                     /gene="sprA"
                     /locus_tag="PKV31_01965"
     CDS             12258..15023
                     /gene="sprA"
                     /locus_tag="PKV31_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNB91647.1"
                     /translation="MNLKINTLSLLFVLGIGLGIYAMATGKIIQGGLDVIQPDEKIEA
                     LDTVIPRKPFYPRDTTNPINLKLPPDLESGYNYDPNTNSYFFSNKIGNDYLDAPTNLT
                     FSEYLKEKSKQQERDYFGKLAGLSDGRNSGKAKDPLEGYKTSNSIISRLFGSDVIDIK
                     PSGNVDIDLGLASNHTFNPTATERQRRYTTFVFQPTINLSIAGKVGEKLSVNANYNTQ
                     STLDFDQFIKIKFDGAKGNEDDILKNVEAGNVSLPLRSSLIQGSQSLFGIKADLQFGH
                     LKLTGLISQQKSERKNLQLQGGSQLQTFSIPIDQYDENKHFLLSHYNRSVFNSSLRTM
                     PQINTFFNITDIEVWVTNDRQETEDVRDIVALADLGEWKHLTNYNPLINDTFAVNHPD
                     YTGRIELPNNDANGLYKQLAITDPENTRPLNKVVSTLQSSPLNMVQTRDFEKVRARKL
                     RKDIDYTFHPQLGFISVNIGLRPDQVLGVSYRYRYNDKIYQVGEFSYDAPVDPDNPNV
                     LFVKLLKATTQKTEIPLWDLMMKNVYSVGAYQTNALDFRLDIFYEDPGYGQKRFLPET
                     DLKGQPLLRVFNLDNLNTTGDPQPDGVFDFVEGVTINSRTGRIMFPILEPFGSDLAKR
                     INNSVLAERYTYPELYTKTITRAREYPEKNRFTLKGSYKSTVSSEISLGAFNVPQGSV
                     RVSAGGTQLIEGQDYIIDYSAGKVTILNDAYLTSGVPVNVSFEDNALFSFQQKSMMGL
                     RADYALNKNINIGATFMRLSERPYAQKVNLGDDPLNNKIYGLDLNFTKDAEWLTRLID
                     KLPLIQTKEPSKVNLTSEVAVIQPGHSKYINNLADEGGTAYIDDFEGSTSSFDLRTPT
                     QEWFIASVPQNDLQNNNPLFKEATLNDNLAQWCALSNAEGAEIKVVVCLGLGQNVLSS
                     FENGRKK"
BASE COUNT         4743 a         3370 c         2902 g         4044 t
ORIGIN      
        1 cgagtcatcc accacttgtc ctgatgtcct tcctttccgg aaggagaaaa tacgtaaatc
       61 ctgttaaaaa gtccggcgct ctccttgacc ggaaaactgg gagcgacgat gatgacacca
      121 tatttacgag ccaaatcgga aaaatgtccc ttgaattcag acagcagatc ctgcatttcg
      181 tctaactgcg tatgaatatc ctgttgtatt tcttttggaa aaagactcac caggtccatc
      241 gaaccatatt ccggaaagag aagaaggtgt tggccctcag cacaaccctc cctgactatg
      301 ccctccattc tttccctcca ttcgtcgagg gaatgataat acacaatcgt atactgatat
      361 gctgcaactt taatctttct gtctgccatg ccggtcaata attatgccag aatcacggca
      421 aaagtactaa aataggctgt taatcgggag tgcatgatcc tttcctcttt taaaaattcc
      481 tcaaccagaa gtccatctcc tttttatcgg gcaaatccat gttcctgtct aaccattcaa
      541 attcggcgat cagctcagga tgctttatat agccccgttt ggtccagaat gcgtcattgg
      601 gtctgtaatt ttcaggccgc agaggatgat ctacaggccg atttacggcg cagaagcagg
      661 tcgtggtgta tgtaccaaaa cttaaggcat gcgcttccct tacatcgaag aaacgatgtc
      721 ccaacccacg cctgcgatat tccggcaaaa gaatactttc accgaaataa aatatcttgc
      781 ccacgtccat cccggccttt acaaaaggca ttttgacctc ttcaacttca tccgacaatg
      841 gcatacaagt ggtagcgcca accatctgat ctccatcgaa cactgcgtgt accagagatt
      901 ttgtacttga agtatagata ctcagatagt ctctctcata atcaaaatca ccctcataca
      961 agtacggaaa ctcataaaat acggcaatac gcaacctcgc caagccgtca aaaagcccgg
     1021 caatctccga ccctgtcttt gtcacaaagg ttaatccatc cgccatcatt ccgaaacctt
     1081 ttcaatccat tcattcagat tgtaataggt cgtcacactc attatccggc cattccgcac
     1141 ggtaagaaaa gctgccgcag gaagtacata gacttgtccg tgtgccggcg ggaggccttc
     1201 ttcacttacc ttgtacttac catgtaccat aaactgaaca gagaccttcc cttcatcaga
     1261 agttgtgtag aacactaatt cctccagccg ctcttcataa gcatcgtcca tcttgtttaa
     1321 aaaatgggca aatagttcct ttccatatct cggcttgccc tcatttggaa aatggacaac
     1381 tgcatcgtcc accaaatcca gcatcccctg ccagtcttta cgattaaagc aatcgtaata
     1441 attttttaca atctctaatg atgtcatatc taatatcaaa attttatttt cttagatcca
     1501 atggctttca caaaaaagaa ttcccaaccc ctccctatcc gtcaccttag gcatgtgatc
     1561 ttgtcaccga ccgatgtttt acccccaact gcattggagc ttgctacact tctctcaata
     1621 catccattca tcacaaacct aactatttcc gtcaaattct tatattacat aatctacaaa
     1681 gaatcctccg attcgtaaaa acatttgaca gataagcttc cttatgaaac accatcattt
     1741 tgtaatttgc ggttcaattt agaaataccc tgtaaaacca gcaaggctga ctttacaagc
     1801 ctttggttgt catccaagga atagttgtca atgaatttga aatactcaaa aaagggtaat
     1861 accgaaggta cattcgttat agaccaattt cattgatcaa tacgactgta gcctcggcgc
     1921 tataccaaaa atttatgtac tataatataa atcatcttgg tataagatgc aatccgatcg
     1981 aaacttaaag tccctaacca atgacaccta tcttcatcat gtgattataa tttttgggat
     2041 ttgatgctga cttcataggt atgtacagga tcttcataag gcttaagggc ctttacttcg
     2101 aaattgtccc gtataaatat cgcacacatg gtattggcga catgatggat ataacgatga
     2161 aaaccgacag gtactgcttc caggatagca tgtagtgttt caaaacgtac acgatccgga
     2221 tcatatgcat aatcatgcca tacaacaatg gaatgttcgt gtatcaggtg atcaaacaca
     2281 ttcctggtat ctgacacaat atactcatgg tgatgattgc catcgataaa gatgacatca
     2341 aatcgcttat ccagtccgga aaaatcaaat acggtagaat ctccaagtaa ctgcgttata
     2401 ttctttcgat ttttcgaaaa aaagccatgc gcatcaatat aatcttcgga caatccgagc
     2461 ctccgcattt gatcatccgc caaattcagg gtaaagcatt cttcggcaac atcagacaca
     2521 ttacgtacac tctcaccacg ccaggtgccg atttcaaaat attttgcctt gtcaaaccgt
     2581 ctgcacaatg ccttcagtaa tgcaatatct gttggaaggg agcctccacc caaaaaggaa
     2641 aaggtttcca gggtttcatg aaagccggga ctaatctgct ccaaactaat gcaagataag
     2701 ccatcaggca aaccaaattc ctgtatcacc ttccgatgcc atactgtatt gtcatccagt
     2761 acattattga gcaaccatgg attgtgtatg agctccctca atgccttgaa agctttctta
     2821 actcctttca tggcccaaat ttcttaaata tttatgaaac aatatcatgg tcatcaatcc
     2881 tgtgaaggta aatgcagcag ccatcccaat agctgcacct tcgattccca tgataggaat
     2941 cagccataca ccaagcccga ggatgatcac tatggccgct aaagaggcat aggtgttcca
     3001 ttgggccttt ccaatagccg aagaaagatt gccataaaga ttgcgcaacc acatattccc
     3061 aaccaatgca gcaaggatga taaaaaacac gatgccattc cctgcatatt gctcaccgaa
     3121 aataaaggga ataatccggt ccctaagcaa aaatccgaca atcaagatga tgatgcccag
     3181 cggtatgaat atcttgtagt aatttttcag ataaaattcc agataactct tatcagcata
     3241 tttgagtgcc agcttaggat aatcagtctg catgaatatt tgcggaagga tagcaagact
     3301 cataggcaga atgatggccg tcttatacat agctattgca tgcccatcca acagaaagcc
     3361 aattaacatg aagtccattg aaaaaagaat atctgacagg aaataggtca ggccaccgtg
     3421 aaaggagtac cgccagaatt ctttcaggtt gatggaaggc attgtgatta tgcctgaaaa
     3481 tgcttccctc ctgtaaaata ggaacgatag ccagggcatg accgccaacg caacgagata
     3541 acccaacatc ccccaaaacc aggtacctgc cagcaccatc aaaaaaccac ccacaccggt
     3601 gatcatacca atccatgcaa acatcttgtt gtcgtgatga atccggaaat aggattctat
     3661 aaaattctga aaataatatc ccaacagacg tactgcaaac aacaagatca caatttggat
     3721 tcctccatat ctaagagtat atatataact aatcgcaatg tatatacata ttattatcaa
     3781 atgattgaaa agtcctttac caaagacata gcgactcaaa ttgtctttgg catcctgaga
     3841 ggtttccagc acaccgtatc taagaagtcc ttgatacgag cccaggccgg ttagaggtgc
     3901 aaatatggca aaaactgaaa atacaagcga aatcaaaccg aattcatcct tagttatcat
     3961 ccgcgctgca ataatattga ttccaagcat ggaaagacgg gagaccagga tggataaaaa
     4021 gaccatcaac cccttattcc ggataaactc acggataaat ctgaacaatt cactcatcca
     4081 aacaaaaatt tgaaactgcc caaaaggctc aacaccattg tcttttcata aaatgtaccc
     4141 tgtaagtaca tcgtcaggca atcctgcttc aaagccggat catgcaccag atcaatattt
     4201 tctgagaaat atcggtcgaa atataacgca atgtcatgat ttgttcggaa ataatattca
     4261 taaggagcca tattgcattt atagggctgt cgaagcagtt tattgtaaat cagattgcga
     4321 aacctgacct taaattgatc gccgaaagcc gtcttccacg tacggtcagg cttcatgagt
     4381 gtgcgttccc aaatataccc ggcagcagaa ggacaaaaat cccgaatcca atctatatat
     4441 attttttcct tgtatctcca tgcctcatca agactcagag caaatctcaa aaaatcattg
     4501 tgcataaacg gggagacagc cacactgaac tgctctgcca catagttgcc gacagcgata
     4561 aaattgaagc cgcgattata aaacaaaaaa gcctcctcgg aagggtattt ggcagcaatt
     4621 cgatccaaaa ccggtctcac tttatccgca tatcgttgat tggcaatgag cttaggaaag
     4681 gcagaagaag ccggattcct ggccggagat gaaaggtagg agcctaaaac agcatctcca
     4741 agatgtcccg tatgaatcaa tcccttatct gcaaaatcaa tagttgacaa tgcaaattcg
     4801 gaatgaacgc ctccggtata acaggccaat ccttgagtga tgccgacaag cttgtcgata
     4861 tgcttcagaa attcaccgcc atctaaatga actacggaaa gggtcaggcc ttccttcccg
     4921 gcaatctgcc ttgctataat ctcatcccaa tatcctgaat gtgaaaagca caatgcctgc
     4981 tcatgacgaa accccatccg gcgcgaataa agtacaccca tccgcgaatc aagcccacca
     5041 ctcaataaag caaagtgtcc gacctggtgc tccaaatcga aggtgtactc ttgacggact
     5101 gcccttgtaa ataattcatc cagggtatga agggctgact ttttatctcc ccgataaagg
     5161 tcctgactac ccggtccctc ccaactttga atttccatct tcctggtctc aacatcaaaa
     5221 gccaactgct ctccaccctt tagcttcctt acctcgctaa tcaaagtgaa attgtccggc
     5281 agactgttga gcgccaatgt acaataggac gcttccatat cgatggaata tagcagagag
     5341 tgcctatcca ggctgcgctt cagggaagcc aaaccggtat cgacaagtaa gaaatcatct
     5401 ctatggtagt aaaaaattgt ttgagatgct gtcaggttgg tatatacaaa tactttacca
     5461 ttacgcttat catataaata tccactgtat tctccggaaa attcgcggga aaatccggtg
     5521 ccaagctttt cgtacaaggc agtaaccaga tgttcgatat ctgcagctgt tccataggag
     5581 gcaatcaatt cattgaaatt aagcaccaca cctttcagaa aaaaacgata ggacggattg
     5641 tcaaagaaat ggtaaaggaa tgactgaaca ccatccacca tttcccgaaa ggtcaattcc
     5701 ttcttaggaa gatgtgatat ctgaaattca cttttcatgc attactctgt tatagatatc
     5761 atgaaagcga ttagccaaat gttcaggtga aaaattttct tcagcaaatc gatgcatttc
     5821 agcaggtgac tcaaactttc gcccccgtat cacagattcc atcgcccgat acagggcgtc
     5881 ctcatcacct ttaggtataa ggatgccgaa tgtttccggc aggaattcac caataccgcc
     5941 gacatctgtg ccaatgaagg gaagaccaca agcgaaagcc tctccctgca cacaaggctg
     6001 gttttcatag ttgctgaaaa gcaggaaaca atcagcattg gccatcattg cagggacctc
     6061 atcatggcta agctcgccga aagggaaaat tacatcctgc aaatgatgtc tcgcgacaaa
     6121 ttgccggatt ggctccaagg gcccgttgcc gcctatatgc aactcaaagg aaagacccga
     6181 atccaccaag cgcttagctg aacgcaagat accactgata tttttatgtt gttcatcgag
     6241 catcgacaaa tgcaaaaaac gaggtttagc ggaagacttc ctttcagaaa agatacggaa
     6301 gcgatcggtg tccacgacat tgggtaccac ttcaaactcc ggcttagaac atacggcctt
     6361 catttgcttc cccagatcag ccgacacagg cagaaccaat cgacaatttt gcagaatgag
     6421 ccgcaccagg atatgtgtca taaggttgag ttgtcgaaac ctgaatggtt gaaatccggt
     6481 ccaatgttca ctcagtatga atggcttgcc aaggaaaaac ttcaggtaga gggcaaataa
     6541 tccggcaggc aacactgtat tcagatgcac aagatccgga acttcggctc tgatataacc
     6601 catccaaaag gctttcatcc ttttgaaaaa attcagcact ttgcaaccgg aatccttgta
     6661 gtatactatg atctcacgca ggccattatc ggtatcactg acctcatagg cacccgcgat
     6721 gtcatcatcc cgaacagtat gcagaacggt aatatctgca atacgggcag caactctggc
     6781 gtgccgttgg ataaaatctc ccgaataagg cgacacacgg gatggatacc aggatgcaag
     6841 gaataaaata cggttttttg gcatgcgccc aaataactgg ttattttggc aaagatagca
     6901 actaaagtaa cggtatacgt aaaaaagctg aaattatact tataactctt tgccctgcaa
     6961 taggtcatca gatcatgatc tttggggatg taaaaaaatg atttcatcct tggttgctac
     7021 ctgataatag gtcaatttac ccattaatat tctgtttact ataagggagt ttggtgccaa
     7081 cccattaaaa gacaacgaaa tatcctgcta aacttttagc aaattgatat taaacgggac
     7141 aataaaggaa ggttgaataa ataaagctgt ggagatgaca ggcaaaagcc gtataaaaga
     7201 atattacaat tcaacaaagt ataaataatt acttaaatat ttatattaaa gcattttata
     7261 tatatttatt ttttacaaat gtaatctatt gatgagagta aggtggaatt acctgtacgc
     7321 tttgtacgaa ttcacatctt atacctgctt ctgacaaagc agtaaaaata tattggaaat
     7381 ccgaaatctt aatttgctga caaacctcaa ccaataatca tttggaaaaa aaccgcaaca
     7441 ttagacaaca agaagtctgg ttgtaataac cttaggacca agcttcattc agaaacagta
     7501 tccagaaacc atagtcacga cctgagaatt atcacagcac aaatatgtga ctgtacaatg
     7561 acacattact gactcattat caatgcactc cctgactcct caccttactg taatgtgcct
     7621 gatcaatttt catcagccag gacttcagat cgtcgattgt cggattgaca ctgatgatct
     7681 gacctttatc attgattaga tatgaggtag gaaccttctt tatctgatac agagtcgtga
     7741 tcgggccacc atcctttggc tccctgaagt gtgagggcca cgctaagctg tccgagttga
     7801 ttacctgcct cagaatagca gaatcatcat ccatggctac tgacaccact tcaaaaatcc
     7861 cgtgcaacga atccctgtaa gtcctataca atttagaaag ctccggcaat tgtttgcggc
     7921 atggactgca ccaggtcccc caaaagtcaa gcaatacaag tttgcctgac atatcatcca
     7981 gtgagaaatg cttgccgtca atagtctgcg cacggaagga aggcgccttt tctccgacca
     8041 tgaatgcagg cttcatatac atatattggt agagaatata gaccaatacg gccagaataa
     8101 agaaaataat atattgaaaa atcttcatca acctggaatt atattacaaa aataactaat
     8161 attaggagaa ttgcccagta tttattttat tttctaaaca ttgaacaaaa aaatatccga
     8221 tatctcaagg ataacgtcat aaaacgatat aaaatgatac ttttgcataa atatttactc
     8281 atgtcaaccc ctgaccattt cgaatatatt gacaaaagta aactacctcg ccacgttgcc
     8341 atcatcatgg acggtaatgg ccgttgggct aagcaacagg gcaagtcgag ggtgcttgga
     8401 cattctgcag gtgtaaagag cgtgcgggaa gtttctgaag ctgcggcccg gttaggcatt
     8461 gaatacctga cgttgtatgc tttctctacc gaaaactgga acagaccaaa actggaagta
     8521 gaagcactga tgttgttgtt ggtggatact atcaacaagg aaatagcgac cttgaagaaa
     8581 aacaatatca gactcaatgc cataggaaat ctggctggtc tccctacagc cagtcagaaa
     8641 gccctgaaaa aaggcattga tgccacaaag gacaatgacg gcctgaatct catccttgcc
     8701 ttgaactata gtggcagaga ggaaataacc aacatggtaa agctaatttc tgaggaggtt
     8761 agggccggtt ccctggaacc cggcatcatc gacaaccgga ttatcgaaca acacctgtac
     8821 acatccggca tgcctcagcc agaattactt atcagaacca gcggagaata tcggatatca
     8881 aatttcctgc tttggcagat agcttataca gaattctact tcactccttt gatgtggccg
     8941 gaatttggac agcaagaatt ttacaaagcc atcatcgagt ttcagtcacg agaacggcgg
     9001 tttggcaaaa tatccgaaca gctctgatcg ataaatttaa caaaacaata agcctgattc
     9061 tgtgtaatag aacacaataa aaacgtttta taatcaaaaa attcaaaatt aatctagacc
     9121 atcggataaa aaacaaacat cttattcgtg aaaatatttt attatcgtgg ttttattatc
     9181 tttgccaaat tacttgaaaa aattgttgac taaattaaca ttcataccac ttattatcat
     9241 gctcatcctg tcaatgaact ctggattagc catgatacac tcaccggatt ctaccggccc
     9301 gaaggttttt ctattgggtg atgatgaaaa tttgatgaat tctctcagta aaaactatcc
     9361 cactttactc atgaaagtgt gtaaggacga tatggaggta gcctacaatg tatggatgga
     9421 aatgctctca gatatggaag catatgccac taaaatcaat tttgatatca gaggcaccaa
     9481 gctgtggatg aacgtctatt ggaataaaaa cggcaaaatt gaatatatcg ggtactatct
     9541 aaagccgaac agcagaaatg taaaaactga ggaaatgaac gctttttttg cgagctttat
     9601 gaaaaattat cagatgaacc tatcttatac cggccgtttt taccacaatg gaagtgcagc
     9661 atttccgact catttcaagg cactcaagga taaagaaggt ggatgacaag acccttttcg
     9721 catgatcaat ccccaacctt attaatttca catcttgttt tgaagcatcg tattgccatc
     9781 ggttgaaaat gccgataaaa aatctgtgtt aattcctgtt tttgcaaaaa agttggatat
     9841 tttattttta tttgtaacca aatattaaaa tatcaataac gtgggagtta aaggcagatt
     9901 gacgataatc agttttatgc agttttttgt ttggggggca tggcttatca ccatggccaa
     9961 tttctggttt ggcactaaaa actgggatgg aacgcaattt ggggcagtgt ttggcactat
    10021 gggcattgca tcaatattta tgcccacact ggtcggcatc atagcagacc gatggatcaa
    10081 tgccgagcgc ctttatgcgc tgcttcacat cctttatggt gccgtattgt tttatcttcc
    10141 caacacaacc gattcgggcg cctttttcta taccatgtta gttgcgatgt gtttttatat
    10201 gccgaccatc gctttgtgca attccatttc ttacaacata ctgaaatcca acaattatga
    10261 tgtaatcaag gaattcccgc ctatcagggt ctgggggaca attgggttta ttgtcgctat
    10321 gtggacgacc aacctcaccg gacagaaagc gacagagggc cagttctata tagcaggagt
    10381 ggcggccatt ttgttgggtt tatatgctct caccttacct aaatgcccac ctcagaaact
    10441 catcgacaaa gatgctcctt tgactcatca actgggatta aacgccttca ggttgtttgc
    10501 tgattataag atggcacttt tcttcatatt ctcgatgatg cttggcgcag cattacaact
    10561 aaccaatgcc tacggagacg tatttctgga cgaattcaaa tatttcccca aatatgcatc
    10621 ttcgtttgta atcaagtatt cgacaattat catgtccata tcacagatat ccgagacact
    10681 tttcatcctt gccatcccgt ttttcttcaa gcgattcggc atcaaacagg taatgctcat
    10741 cagcatgctg gcatgggtgt tcaggttcgg tttatttgga tttggcgatc ctgcaggtgg
    10801 gctttggatg atcatcctat catgcattgt ctatggtatg gccttcgact tttttaatat
    10861 ttcaggatct ttatttgtag aaacaacgac tgaacctaag attcgttctt ctgtacaggg
    10921 cttgtttatg atgatgacta atggtttcgg agctgtgttg ggtagtgtca ttgccggctg
    10981 gtccattgat cggttcttca ccaaaaaatt cattgatatt caatctcttg cagccaactt
    11041 tgatacaaca gcggacaatc caacctttct cgatattgca aaaaatgctt tgggggcagt
    11101 gatcaatcaa gatggcagcc ttgctaatcc cgtatatatt aaggactggc atcagatatg
    11161 gattgcattt gccgcgtatg cactccttgt caccatactt tttgctctcc ttttcaaaaa
    11221 tgagccaaaa caagagcttg atctgagtaa attgaaccat tgatacaaaa gtaaatacct
    11281 gtattcagac agttggagta ccagtaaccg cgtatgttaa gaaaaggata ttgtcaacta
    11341 tacttcatga caagaagagc tttagtcaac aaatcctgaa gaatatattg taccattgat
    11401 tttaataaat ttactttttg tataatttga ttacctttgc acgctgaaaa ttagggcgaa
    11461 ttgcaatgat cgcatatatc catggcaaaa ttacgggact gacaccgact tatgcattga
    11521 tagagacatc gggcatagga tatcacgtca atataagcct caatacctat gcgatgctgg
    11581 atgggaagga gacttataag ttgcttactg ttctgctgat caaagaggat tctcatactt
    11641 tgtatggctt tgcaaccgaa gaagaacgaa gtgttttcac gcatttgatt tctgtcaatg
    11701 gtgtgggtgc aggtactgcc agacttatac tttcctctat gcctgcaacc gatgtcaagc
    11761 aggcaatcat acgagaggat gaacgcatgt tctcaatggt caaggggatt ggcccaaaaa
    11821 cggctaaaag aatcatcata gatcttaaag acaagctaag caaagaaggt gaccttccgg
    11881 ccggaaatat ccgattgcct ctggtatccg ccatccggga tgaagccggt gcggcacttc
    11941 tggcacttgg ttttcaaaaa aataacatcg acaaggtact caatacatta ctgaaagaag
    12001 acaaagaata taccttggag cagatggtaa aggctgcatt gaagagctta tcaggtgcat
    12061 aaaatgttaa ttttattaag aataaaagaa aaaaggaact ttttcgtttt gaataaaaca
    12121 tatatttata tgaccacaat tatgaataat taacattcgc cccgaacaat taaaaattcc
    12181 attaataatg ccggatcttg tcggttcgga taaaattaaa ataaaaagcc gttgatataa
    12241 cgcgttgata aatttatatg aatttaaaaa tcaacacttt aagtctatta ttcgtcctcg
    12301 gtatcggatt gggaatatat gctatggcga caggcaaaat aattcaggga ggcctcgacg
    12361 tcattcaacc ggatgaaaaa atcgaagccc ttgatactgt cattccccgt aagccattct
    12421 acccgcgcga caccaccaat ccaatcaacc tcaaactccc tcctgatctt gaatccggct
    12481 acaattacga tccaaatacc aatagctatt ttttctccaa taaaataggt aatgattatc
    12541 tggatgcccc caccaatcta accttcagtg aatacctcaa agaaaaatca aagcaacagg
    12601 aaagggacta tttcggcaaa ttagccggat tgtccgatgg caggaactcc ggcaaagcca
    12661 aagacccact ggaaggttac aaaaccagca actccattat ttcgcgcctt tttggcagtg
    12721 atgtaataga tatcaagccc tccggaaacg tggacatcga ccttggtctg gcttccaatc
    12781 acacctttaa ccctactgcg actgagcgcc aaagaagata tactactttt gtattccaac
    12841 ctactatcaa tcttagcata gccggaaaag taggagaaaa gcttagtgtc aacgccaact
    12901 ataatacaca gtctactttg gactttgacc agtttatcaa aataaaattt gatggcgcaa
    12961 agggcaacga agacgatatt ctcaagaatg tagaagccgg taatgtatcc ctgccgctgc
    13021 gaagctcgct gattcaaggt agccaatcgc tattcggtat caaggctgac ctgcaatttg
    13081 ggcatctcaa gttgacagga ttaatttctc aacaaaaatc agagcgtaaa aacctacaac
    13141 ttcaaggtgg aagccaattg cagacattct ccatccctat tgaccaatac gacgaaaaca
    13201 aacacttcct tttatctcat tacaacaggt cggtgttcaa ttcatccttg aggaccatgc
    13261 ctcagatcaa tacattcttc aacattacgg atattgaagt atgggtaacg aatgaccgac
    13321 aggaaacaga agatgttcgc gacatagtag ctttggcaga cttgggcgaa tggaagcatt
    13381 tgactaatta caacccttta attaatgaca cttttgcagt caaccatcct gattatactg
    13441 gcagaatcga actaccgaac aatgatgcca acgggttata caagcaactg gctattactg
    13501 acccggaaaa tacaagacct ctgaacaaag ttgtgagtac gcttcaatcc tcaccattga
    13561 atatggtcca gacaagggac ttcgaaaaag taagagcccg aaaactgaga aaggatatcg
    13621 actatacctt ccacccacag cttggattta tatctgtaaa tataggtttg aggcctgacc
    13681 aggtcttagg agtttcttac cgataccggt acaatgacaa gatataccag gtgggtgaat
    13741 tttcatatga tgccccggta gaccccgata atccaaatgt tcttttcgta aaactcctca
    13801 aagcaacaac acagaagact gaaatacctc tttgggacct catgatgaaa aacgtttatt
    13861 ctgtaggtgc ctaccaaacc aatgcacttg attttcgatt ggatattttc tatgaggatc
    13921 cgggttatgg tcagaagaga ttcttaccgg aaactgatct gaaagggcaa cctttgctga
    13981 gggtgttcaa cctcgataac cttaacacga caggagatcc acagccggat ggcgtttttg
    14041 actttgtcga aggggtaaca atcaacagtc ggaccggccg tatcatgttc ccaattctgg
    14101 aaccttttgg ttcagatctg gccaaaagaa tcaacaactc tgttctggca gaaagatata
    14161 cctatcctga actatataca aagacaatca cacgtgcacg ggagtaccct gaaaaaaaca
    14221 ggtttaccct aaaaggttcc tacaaatcaa cggtttcatc ggaaatttca ttgggtgcat
    14281 ttaacgtacc acaaggttcg gtgcgtgtat cggcaggagg aactcaattg atagaaggac
    14341 aggattacat tatcgactat tcagccggta aggtcacgat cctcaatgat gcatacctga
    14401 cctcaggtgt accggtcaat gtctcgtttg aagacaatgc attgttcagc ttccagcaga
    14461 aatcaatgat gggactgcgt gccgactacg ctttaaataa gaatatcaat atcggggcta
    14521 ctttcatgcg cctttcagaa cgaccttacg cacaaaaagt caatctcgga gacgatcctc
    14581 tcaacaacaa aatatatggt cttgatctca acttcacaaa ggatgctgaa tggttgaccc
    14641 gactgataga taagttgcca ttgattcaaa caaaggaacc gtccaaagtt aatctcacct
    14701 cagaggttgc agttatccag ccggggcact caaaatacat caacaacctg gcagacgaag
    14761 gcggaacagc ctatatcgat gactttgaag gaagtacttc cagttttgat ctaaggacac
    14821 cgacacaaga atggtttatt gccagtgttc ctcaaaatga tttgcaaaac aacaatccac
    14881 tgttcaagga agccaccctg aatgataatc ttgctcagtg gtgcgcgctg tcaaatgcgg
    14941 aaggggctga gataaaggtc gttgtttgtt tggggttggg tcaaaacgtt ttatcttcct
    15001 ttgaaaatgg gcgtaaaaaa tagttttttg tgttgtcatt attaattgag ttattaagc
//
LOCUS       DAOQMC010000026        14890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1465_length_14890_cov_4.310010, whole genome shotgun sequence.
ACCESSION   DAOQMC010000026 DAOQMC010000000
VERSION     DAOQMC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14890
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_14890_cov_4.310010"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKV31_01970"
     CDS             complement(<1..639)
                     /locus_tag="PKV31_01970"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNB91648.1"
                     /translation="MNLLEKINETATYISNRINESPTVGIILGSGLGTLADAVEVSSE
                     LNYTDIPHFPVSTVIGHEGKLIFGKLGGKQCVMMKGRFHYYEGYDMLTVTFPIRVMKA
                     LGVQTLVVSNAAGGMNAAFQVGDIMLIRDHINQFPEHPLRGPSDKRLGPRFPDMSEPY
                     SLSLLKIAHQAASDLNLKVHEGVYVGVQGPTFETRAEYKWLHIIGGDAVGMST"
     gene            complement(803..976)
                     /locus_tag="PKV31_01975"
     CDS             complement(803..976)
                     /locus_tag="PKV31_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91649.1"
                     /translation="MRKNKKMPMYNKRKIYYSDMTKGLEAIESRVFCNNRFHYEEQFV
                     ANISNEFWVQPDI"
     gene            complement(1674..3434)
                     /locus_tag="PKV31_01980"
     CDS             complement(1674..3434)
                     /locus_tag="PKV31_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB91650.1"
                     /translation="MSFLLKYLRKHTWLIILTLLLAAIGQIFSMMDPYIMRLLIDGYT
                     KDLDKFSSDDFIRGVLTLGLAIIGVAMVSRIAKAFQDYMVNLITQKVSTEIYNDGLQH
                     ALSLPYEDFEDQRSGETLGILQKVKTDVQRLLSTTINILFVSLVGIVAVTIYSFSVYW
                     AVGPLFIITIPVLIVLSGYLTKKIKKIQKRIVSETTALAGSTTESLRNIELVKSTGLV
                     DQEINRLNDTTNRILDLEIQKVKYVRSLSFIQGTVINFLRTSIIVIMAWLIFLGKISV
                     GEFFSLFMYSFFIFGPLQEIGNLINIYSETLVSLQNFKQLMARKPEMAPEQPVHIDSI
                     DNITFNNVCFTHQSANKPALSNISFDVRKGEKIAFVGPSGAGKSTLIKLLFGLYKPKK
                     GRVAYSDIPIESIDINDIRHQIGIVNQETHLFAGTIRDNLLFVKPDASDEELEKVLRM
                     AAADSLLDRADKGIYTTIGEGGIKISGGEKQRISIARALLRNPNLLVFDEATSALDSL
                     TEEEINETIEHLPGNEGRITFLIAHRLSTIMHCDRIYVLESGRIVESGKHTELLDQKG
                     LYYAMWRQQVGLEPEPEEVF"
     gene            3705..9086
                     /locus_tag="PKV31_01985"
     CDS             3705..9086
                     /locus_tag="PKV31_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNB91651.1"
                     /translation="MAKQIPPRKRNTRSNNPLTNPSSDQEVQHSVDPENRDERIIITG
                     IVGDITPEYSLDGFTPVMSVELDASRGVASEDIEVDSNLLAQVVFSDGTVWCGYVGDL
                     PEIYTQMGISRGAGGSMKLPELLTSTSDGRGDGGVFRRLVNVFRPEAMSYTAHTIALK
                     VDRNIMAAPGLYTVDASFQLLPLKDGAILSGTVLLLLHGTLSSTKGSFGDMTTGGMWE
                     AIYSKYDHIIALEHHTISETPLKNARDVLQALPPGLSVDILSQSRGGLIGDVLTRCDY
                     RNDHPGYSSDERAILVREDKTLGVLVDELNGLAKEKRLTVRRFIRVASPSGGTSVLGQ
                     RMDIFLNAFLNITGLVFGVGVTPVYQAVREFIMEVLSHRFKASDMPGMWSMVPSSAYQ
                     RVNNMQHDYHTEVFVIAGDGKAGGKITNSLLVIFTNLYYRAPNDFVVDTASMNKGVSG
                     PSGIQYFLSNTNKTNHFGYFRNEDSRWALQYAFRMVNNGEESPFSDVGGDKDSSRGIG
                     LDLVMKRYSTNKVSGTRPIVIILPGIMGSTLCDNVDDIWLSIPRISAGAFVNKLNINA
                     TNIGTSGVMEKYYRKIGEFMSLNYDVEVFPFDWRKDLTESARLLAAELKRCLSFGKPV
                     QVIAHSMGGLVMKQLMIKEKSTWHSFCQSSSNRLILLGTPWLGSHLIMEVLTGHSSKV
                     KLLGLLDKIHDREQIMSTAAGFPGVIQLLPMTDEGFGTSVFWENFRPYTNHLAVPPSH
                     MFDLYNNYKQETAGFRMDEAHLDNVFYIAGHDETTSSYKIEWKFLQGKRLVFRSTDAG
                     DGSVTWKLGIPPELKRSNLYYSEITHGELANKQIIIDTIMDIFLSGKSSRLPQTEPGS
                     RGIQQEGIVPEFDFIPNNDDELVDALFQGKIGRREAKPQTEPLQVSVYHADLKYSSHP
                     VLVGHFLNDGLYSAEKSLDSYLGNKLSERLRLGYYPGNIGECEITYQSESRPKGALVA
                     GLGLADELTSYRLSLTVESAIIKYAFFFRDNYSRSENKRVASGISTVLVGSSFAGIPI
                     NECLRAILSGIQRANERMVQLNIGLQLIRQVEFVDYYEDLAQQCYSILKDFERKSDDF
                     NIEVAPFRKGPGMRRRLTLGDTSSWWRTFNTVTNFDRLTGRPSGFTFSLTSGRARIEQ
                     GGVTTDLKLVEYLAKEYSHKNDWNPRLSKTMFELLIPNDFKNIIRNQSNMVWKLDEYA
                     AQFPWELFHDYNDITNAHREKPAFVNTGMIRQLITEDFRRNPTVVTEDKALVIGDPDY
                     SGTVFSQLQGAEEEARVIGKKLVEDNFEVRSMIHSRAGDIITELFSDKYKIIHIAVHG
                     VYGEVAVNDSKEFKAGVVLGNDVYLEASMINQLSTIPEFIFINCCYSGDMTGLDEKYF
                     KERSALAANIGTQLVRMGVKAVIVAGWAVHDGAALTFSTELYDRMLSGFEFGEAVLSA
                     RRKCYDKHPDYNTWGAYQCYGDYFYKLTGKSSMRVADAPFVTESHAIIELQNEISLIS
                     GRKVDKPIHMRILEGLKARVDAGNVSSGAVKELFANAYALLGEYKVAVELYSKLREVE
                     AADFSVSSLEQYCSLRGKIILEDFKAGRGTDEAGIERLIEDMRLIDLIGNTSERKALK
                     GSLYKRLSYIRKEDAKKKRSCLGLMKYFFGEAFCLMRSMEEADRVYSLTNYLLAAYLE
                     KGRIEWKDIQKLGIQGYSEVIEMLNGYADRLKSDSEKYKDYYRANALAKIYLVRMLYI
                     DKQESLDQEITIYRKALMDSLVSFANLKQILGEVEFLDYITGMLPKKDKKKIEALIKV
                     KVDVASLLT"
     gene            complement(9223..9891)
                     /locus_tag="PKV31_01990"
     CDS             complement(9223..9891)
                     /locus_tag="PKV31_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB91652.1"
                     /translation="MAHKVKIKSIAPITHDVLRIVAERPEGLIYTPGQAVDISINQEG
                     WDKEIRTFTFTSLPSDDSIEFTIKTYQAHNGVTNYLRELHAGDEINLYDVYGDIHYAG
                     EGIFIAGGAGITPFIAILKQLEAEGKLGGNKLIFANKTKADIIDADRFEKLLGDKFIN
                     VLSDEKIPGYEHGFITAELIRKNSDPQRHYYYLCGPPPMMDAVQRHLASLGIAESHII
                     KEGF"
     gene            complement(9908..10543)
                     /gene="folE"
                     /locus_tag="PKV31_01995"
     CDS             complement(9908..10543)
                     /gene="folE"
                     /locus_tag="PKV31_01995"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNB91653.1"
                     /translation="MQKTIPLNHREVSSETKHSPEFNGLTDEEKIRKIKYHFAQIMDA
                     LGLDLSDDSLMDTPERVAKMYVKEVFSGLNPANKPVISLFDNKYQYNKMLIEKDITLF
                     SYCEHHFVPIIGRAHVAYIPDDKIIGLSKLNRIVQYYAKQPQVQERLTIQIADALKEI
                     LQHDNVAVMIEADHLCVASRGIKDTNSKTITSMLSGRFEEDSVRNEFLSYV"
     gene            complement(10547..11194)
                     /locus_tag="PKV31_02000"
     CDS             complement(10547..11194)
                     /locus_tag="PKV31_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB91654.1"
                     /translation="MVIHDILNEKEIISRILNGEKSLYEIIVRRYNPFLYKLGRAYNY
                     NHDDTQDLMQDTYIDAYKNLGQYKGIANFKTWLFRIMLNNCFRKKEKLSYKNEVMQDI
                     NDNSKPLFSHSGHDTERIVHNKELGEIIEKALSEIPFDYRMAFSLREINGLNVAETAE
                     LLDISEANVKVRVNRAKNMLREIIEKNYIGEELFAFNLIYCDAMVNNVMNIVNEL"
     gene            complement(11377..11757)
                     /locus_tag="PKV31_02005"
     CDS             complement(11377..11757)
                     /locus_tag="PKV31_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91655.1"
                     /translation="MATYNETIIKKTTHLLRYTFTILPIAAGTDKFLNMLTDWTAYIN
                     PGFEEILPFSGSVFMMIVGVIEIIAGIIVFTKPKTGGLVVAAWLIIIALSLLAGWNHV
                     DVAVRDLVMAIAAFSLSNLVKASE"
     gene            complement(11899..12561)
                     /locus_tag="PKV31_02010"
     CDS             complement(11899..12561)
                     /locus_tag="PKV31_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91656.1"
                     /translation="MKNALKNFKLSSINRYAGILIAAAIMTFAFQSCSKESIDPTATA
                     SYKVLNANMRKLWSDHMQWTYSTVDAFFNNPDGLNGPLNRLLQNQKDIGAAIIPYYGE
                     DAGGQLTKLLTDHITGAVPVLTAAKEGNNEALTTALNDWYKNAQDIADFLANANPNWK
                     QEDMRHMMKTHIDQTTEYAVDLLQKDYSKAIKVFDEANEHMLMMADDLSEGIAKQFPE
                     KF"
     gene            complement(12713..13408)
                     /locus_tag="PKV31_02015"
     CDS             complement(12713..13408)
                     /locus_tag="PKV31_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91657.1"
                     /translation="MQTTITSNKIKGTKQKESIIIKTQIKRFAAFLILLVSLQSCHKD
                     DIETNPLDSTKYKIFSNSMRKLWSDHATWTRNLIIDIAEDAPGTAVDFTRLLKNQEDI
                     GEAIKPYYSEEAGDKLTYLLKEHIVIARNVFTATKSRNTDDLNLMIDSLYENGDAIAM
                     FLSTANPMNWKSHHMKEHMKEYIDLTLSEATNHLNGNYQGSIEAYDEIYSQLLHLADQ
                     LSSGIALQFPEKF"
     gene            14026..14553
                     /locus_tag="PKV31_02020"
     CDS             14026..14553
                     /locus_tag="PKV31_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HNB91658.1"
                     /translation="MNRRTLVSFDWALKRLLRNKANFNILSGFLSELLFDDICVEEIL
                     ESEGNQDSDRDKFNRLDLLCPNQRNEFIIIEVQYHDESDYFHRCLYGTSKVITEYINK
                     GEPYHQVKKVYSVNILYFDLGHGDDYIYVGKTKFTGLHNKSNLTLSPTQQKKFDKSEP
                     FEIFPEYILLKINNI"
     gene            14672..>14890
                     /locus_tag="PKV31_02025"
     CDS             14672..>14890
                     /locus_tag="PKV31_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91659.1"
                     /translation="MDKVSKQQYDQYLKNMRISQDALDTALEKGLIKGRLQEKIDIVL
                     NCLSNGIDIHLIANITNLSEEEVEKIIRE"
BASE COUNT         4153 a         2874 c         3210 g         4653 t
ORIGIN      
        1 agtgctcata cccaccgcat cgccgccgat gatgtgcagc catttatact cggcacgggt
       61 ttcaaaggtc ggtccctgca cgcctacata gacaccctca tgcaccttga gattaaggtc
      121 ggaagctgcc tggtgagcta tctttagcaa tgacagggaa tacggttcac tcatgtcagg
      181 gaatctcggt cccaaccttt tgtctgatgg gccacgtaag ggatgttccg gaaattggtt
      241 gatatgatcc cggatcagca taatgtcgcc gacctggaat gcagcattca tacctcctgc
      301 tgcgttggat acaacaagtg tctgtacacc cagggctttc attacacgaa tgggaaaggt
      361 tacagttaac atatcataac cttcataata atggaagcgg cccttcatca ttacacattg
      421 cttcccgcca agtttgccaa atatcaattt cccttcatga ccgatgacgg tggaaacagg
      481 aaagtgggga atgtcggtat aattcagttc gctacttact tcgactgcat ctgcaagcgt
      541 gcctaagccg cttccgagaa taatgcctac cgtaggcgat tcgttaatcc gattgctgat
      601 gtaggtggcg gtttcattga ttttttccaa tagattcatg tcgtatgtgc ttaatggcgc
      661 caataaatgt agggcaatat aatgaatatg actgataaat tttttcgtga tttgaagtca
      721 agattggctc ttatttcaat gcaagattat aaaatgcagt aaataaataa aatatgcatg
      781 ataaaataac cgtatatttt aatcatatat caggttgcac ccaaaactca ttagagatgt
      841 ttgcgacaaa ttgttcctcg tagtggaacc tgttgttgca aaagactctg ctttcgattg
      901 cttccaaacc tttggtcata tcagaataat aaatcttacg cttgttgtac atgggcattt
      961 ttttgttctt tctcattcga aatccgggat aaatacccgt cttttcattt tatccttgtc
     1021 tgtggaattc agtttgatta aagcattctt cttccctgta cgatcattgt acagctcttt
     1081 ttgtagatca tcagattttt caaatacatt tggtgtcttt tagcttaagg ttgattgaag
     1141 cttcagatat ctataaatcg cttttttgca tccgccgcat tccctcaatt cctgagtcct
     1201 tgattatttc attcatttta attctcgctg aacagcctgt tgatgcataa attgcttgtt
     1261 atatatgctc ctgcattgac ttatgatata ttaatcatct aattaatgca ttttttttaa
     1321 atcaactttt gacagactat tctttccggt tcattttctc acttctttgc aatttgaagg
     1381 cttgaggagc actttatata ttcttgctat tcaataattt atttggtaaa acataaagtg
     1441 ttgctacaac acgcgatgga tatttgaatt ttcttttgag tcagtttttt cttaaatgag
     1501 gtgcactaaa ttaccctgct tcttataaaa tgtgggttgc accaaaaatt tttggactat
     1561 tatattaatt catctgggta aaatttctcg aaaacttcag aataaagcta gtatttgcgg
     1621 gattcctttt gcttttcagt aaatttactt ggttgttttt tcaacctttt tacttaaaaa
     1681 acctcttcag gttcaggttc caacccgacc tgctgtcgcc acatggcgta atacaagcct
     1741 ttttgatcca aaagttcagt atgcttaccg gactcgacga ttcgcccgct ttcaagtaca
     1801 tagatgcggt cacaatgcat aatggtactc aggcgatgag caatgagaaa ggtaattctg
     1861 ccttcattac ccggcaggtg ctcaatggtt tcgttaattt cctcttcggt tagactatct
     1921 aaggcagagg tggcctcgtc aaaaaccagc agattgggat tgcggagtag cgctctggct
     1981 atagaaattc gctgtttttc accacctgag atttttatac cgccttcacc gatggtagtg
     2041 tagatgccct tatctgctct gtccagcaaa ctatctgcag ccgccattct caagaccttt
     2101 tccaattctt cgtctgaggc atccggcttg acgaaaagca aattgtcgcg gatagtacct
     2161 gcgaagagat gtgtctcctg attgacaatg cctatctggt gtcggatatc gttgatatcg
     2221 atcgactcta tcggtatatc ggaataggcg acccgacctt ttttgggttt atacaatcca
     2281 aataacagct ttattaaagt actctttccg gcacctgatg gcccgacaaa ggcgatcttt
     2341 tcccctttcc ggacatcaaa ggatatattg gaaagtgcag gtttgttggc actttgatgc
     2401 gtaaaacaaa cgttgttgaa tgttatgtta tcaatcgagt cgatatggac aggttgttca
     2461 ggtgccattt ccggtttccg ggccatgagt tgcttaaaat tctgcagact tactagtgtc
     2521 tccgagtaaa tatttatcag atttcctatt tcctgtagcg gaccgaaaat gaagaaactg
     2581 tacataaaca aagaaaagaa ttcacctacg cttatctttc caaggaatat caaccatgcc
     2641 ataatgacta tgatactggt tcgtagaaaa tttataactg taccttgaat aaaggaaagg
     2701 cttcgcacat atttgacctt ctgtatttca aggtcaagga ttcggttggt ggtatcattg
     2761 agccgattga tttcctgatc taccaggccg gtacttttta caagctctat attgcgcagg
     2821 gattcggtag tggagccggc taaagctgta gtctctgaga caatacgttt ttgtattttt
     2881 ttaatttttt tcgtcagata tccacttagc acaatcagga ccgggatagt tatgatgaag
     2941 agtgggccca ccgcccaata aaccgaaaac gaatagatgg tgacagctac aatgcctact
     3001 aatgagacga aaaggatatt gattgtggtg gaaagcagtc tttgtacatc tgtcttgact
     3061 ttttggagga taccaagtgt ctcgccgcta cgctgatctt caaagtcttc atagggtaga
     3121 ctaagggcgt gttgcaagcc atcgttataa atctcagtac ttactttttg tgtaatgaga
     3181 ttgaccatat aatcctgaaa agcttttgca atcctggaaa ccatggcaac gccgataata
     3241 gccagcccaa gggtcaaaac accacggata aagtcatcac tggaaaattt atccagatcc
     3301 ttagtatacc catcgattaa caatcgcatg atgtacggat ccatcatact gaatatctgg
     3361 ccgatcgctg ccagaagcaa tgtcagtatg atcagccagg tatgttttct aagatatttt
     3421 aacaggaatg acatcagaaa atttacattc aaacaagcta atcatccgtt ggtttaatca
     3481 tcacaatgaa tgtagcctaa tttatattca tttaattgaa aatttgcatt agtttggtta
     3541 tatttggcaa atattaccat gaaaatcaat cctcatatct tttaaacgag ggttaaattc
     3601 tttctttaag tagggtcttg aaatatttct gtaaatagct gtatttgtaa tgtcagatgc
     3661 agtgcttcac ctgtgatggt gattaatccc aaatatgagt aattatggca aagcagattc
     3721 ctccacgaaa gagaaacaca aggagtaata atccacttac gaatccatct tccgaccagg
     3781 aagtacagca ttctgttgat cccgaaaatc gggatgaacg tattattata accggtatag
     3841 tcggagatat aacacctgag tattcattgg atggtttcac tccggtgatg tctgttgaat
     3901 tggatgcttc ccggggagtt gcttcggagg atattgaggt ggatagtaat ctgttggcac
     3961 aggtagtgtt cagtgatggt acagtctggt gtggctatgt cggcgatttg cccgagattt
     4021 atactcaaat gggcatctcc agaggtgcag gtggatcaat gaagctaccc gaattgctta
     4081 cttctacttc ggatggaaga ggggatggtg gcgtttttcg taggttggtc aatgtattca
     4141 ggcccgaggc aatgtcttat acagcgcata caatcgccct taaagttgac cggaatataa
     4201 tggcagcacc cgggctttat actgtagatg cgtcttttca actccttcct ctcaaggatg
     4261 gtgcaatttt gtctggtacg gtacttttat tgttgcacgg caccttgagt tcaacaaaag
     4321 gctcttttgg ggatatgact accggtggca tgtgggaggc aatctacagt aagtatgatc
     4381 acatcattgc tctggaacat catactattt ccgaaacacc tctgaaaaat gcgagggatg
     4441 tgctgcaggc tctgccaccg ggattatcgg tagatatact ttctcagtcg cgcggtggcc
     4501 tcataggtga tgtcctgacc cgatgtgatt atcgcaacga ccatccgggc tattcgagtg
     4561 atgaaagggc aatattggtt cgggaggata aaacattagg tgtattagta gatgaattga
     4621 atggactggc gaaagagaaa agattgactg tgcgtcgttt catcagggtt gccagcccaa
     4681 gtggtggaac atcagtgttg ggccagagga tggatatctt cctcaatgcc tttctgaata
     4741 tcacagggct tgtattcggc gtaggtgtaa caccggttta tcaggcagtg agagagttta
     4801 ttatggaggt cctttctcac cggtttaagg catcggatat gcctgggatg tggtctatgg
     4861 tgccttcctc agcctaccaa cgtgtcaaca acatgcagca cgactaccat acagaagtat
     4921 ttgtgattgc cggtgacggt aaggctggcg gtaagataac caattcactg ctggttatat
     4981 tcactaacct ttattaccgg gcgccaaatg attttgtagt cgatactgct tcaatgaata
     5041 aaggtgtaag cggtccatcc ggcatccagt attttctttc gaataccaac aagactaacc
     5101 attttggcta tttcagaaat gaagattccc gttgggcgct gcaatatgcc ttcagaatgg
     5161 tgaataacgg tgaggaatcg ccattttccg atgtaggggg cgataaagat tccagccgcg
     5221 gcattggtct ggatcttgtg atgaaacgtt attcaaccaa caaggtgtcg gggacacggc
     5281 caattgttat cattctgccg ggcatcatgg ggtcaaccct ctgtgacaat gtagatgata
     5341 tctggctatc cattcccagg atttctgcag gtgcatttgt caataagttg aatatcaatg
     5401 ccactaatat tggaactagt ggagtcatgg agaagtatta cagaaagatc ggggaattca
     5461 tgtctttaaa ttatgatgtt gaagtttttc cttttgattg gcgtaaagac ctgacagaat
     5521 ctgccaggct gcttgctgcg gagttgaagc gatgcctcag tttcggaaag ccggttcagg
     5581 tgattgctca ttccatgggc ggtttggtta tgaaacaact gatgatcaaa gaaaaatcga
     5641 cctggcattc attttgccaa tcgtcatcga atcgcttaat actcctgggt acgccatggc
     5701 ttggttcaca tctgatcatg gaagtgttga ccgggcattc atccaaggtt aagttgctgg
     5761 gtcttttgga caaaattcat gatagagaac agattatgtc cactgccgcc ggtttccccg
     5821 gagttatcca gttactgcct atgacggacg aaggctttgg cacttcagtc ttttgggaga
     5881 atttcagacc ttataccaat catctggctg tccctccttc acatatgttt gatttatata
     5941 ataattacaa gcaggaaact gccggcttca ggatggatga agcccacctg gataatgtct
     6001 tttatattgc cggccatgat gaaacaacct ccagctataa aatcgaatgg aagtttctac
     6061 aaggtaaaag gttggtcttt aggtcaacag atgccggtga tggcagcgta acctggaaat
     6121 tgggcattcc tcccgaacta aaaaggtcca acctttatta cagtgaaatc acacatggcg
     6181 aattagctaa caagcagatc atcattgaca caatcatgga catatttttg tccggaaaat
     6241 cttccagact accccaaact gaacctggca gtcggggtat tcaacaagaa ggcattgtac
     6301 ccgagtttga ttttataccc aacaatgatg atgagttggt tgatgctctc ttccagggca
     6361 agattggccg aagggaggcg aagccacaga ctgaaccatt gcaggtgtcg gtatatcatg
     6421 cggacctgaa atattcgtcc catccggtat tggtcggaca ttttctcaat gatggtcttt
     6481 atagcgctga aaaaagtctg gacagttacc ttgggaacaa gctaagtgag agacttcggc
     6541 ttggctatta tcccggcaat atcggtgaat gcgagatcac atatcaaagt gaatcaaggc
     6601 caaaaggcgc attagttgcc ggtttaggat tagctgatga attaacatcg tatcggcttt
     6661 ccctaaccgt cgagagtgcc atcatcaaat acgcattttt ctttagggac aattacagca
     6721 ggagtgagaa taagagggta gcatccggga tatctactgt actggtaggt tcatcatttg
     6781 caggtattcc gattaacgaa tgcctccggg ctattttgtc aggtattcaa agagctaatg
     6841 aaagaatggt tcaacttaat atcgggcttc agttgatccg gcaggtggaa tttgtcgatt
     6901 attatgaaga tctggcacaa caatgctaca gtattttgaa ggatttcgag cggaaaagcg
     6961 atgatttcaa tattgaggtt gcaccattca gaaagggacc cgggatgagg cgtcggctta
     7021 ccctcggcga tacatcgagc tggtggcgga ctttcaatac agtaactaat tttgatcggc
     7081 ttaccggacg accatctggc tttactttca gcctcacctc cggacgcgca agaatagagc
     7141 agggtggggt gactaccgat ctgaagctgg ttgaatatct ggcaaaggaa tattctcata
     7201 agaacgattg gaatcccagg ttgtctaaaa ctatgtttga acttcttatc ccaaatgatt
     7261 tcaaaaacat catccgcaac caaagcaaca tggtatggaa actggatgaa tacgcggctc
     7321 aatttccatg ggaattgttc cacgattaca atgacataac caatgcacac cgggaaaaac
     7381 ctgcctttgt caatactggt atgataaggc agttgattac agaagatttc cggcgtaacc
     7441 cgacagttgt tactgaggat aaggctctgg ttattgggga tcctgattat tcggggactg
     7501 tattttcgca acttcaggga gcagaagaag aggcaagggt aattggaaaa aagttggttg
     7561 aggataattt tgaagtcaga tcgatgattc attcccgggc gggagatatc atcactgagt
     7621 tgttttctga caaatacaag ataatccaca ttgcggtgca tggtgtatat ggagaagtgg
     7681 ctgtgaatga ttcgaaggaa ttcaaggccg gggtcgtatt aggcaacgat gtatatctgg
     7741 aggctagcat gatcaaccag ctatcgacaa taccagagtt tatatttatt aattgctgtt
     7801 attctggcga catgaccgga ttggatgaaa agtattttaa ggaaaggtct gcgcttgcag
     7861 ccaacatcgg tacacaattg gtacgaatgg gtgtcaaggc ggtaattgtc gctggttggg
     7921 ctgttcatga tggtgctgca ttgacattct cgacggagtt gtacgacagg atgctgagtg
     7981 gttttgagtt tggagaagct gtactctctg cacgccgtaa atgctatgac aagcatccgg
     8041 actacaacac ctggggagca tatcaatgct atggagatta tttctataag ttgacaggca
     8101 aatcttcaat gcgtgttgca gatgcccctt ttgtgacaga atcacatgcc atcatcgaac
     8161 ttcagaacga gatttctctt atttcaggca gaaaagtcga caagccaata catatgagaa
     8221 tcctggaagg cttgaaggcg agggtcgatg ctggtaatgt ttcttccggg gcagtcaagg
     8281 aattgtttgc gaatgcctat gccttgttgg gagaatacaa agttgccgtg gaattgtata
     8341 gtaaattaag ggaggtagaa gcggcagatt tttcagtatc atctttggag caatattgca
     8401 gtttgcgggg taagatcatc ctggaggatt ttaaagccgg caggggcaca gacgaggccg
     8461 gcatagaaag gctgattgag gatatgcggt tgattgactt gataggtaat acgtctgaaa
     8521 gaaaggcact gaagggctct ctttacaaga ggctttccta tatccgtaaa gaagatgcta
     8581 aaaagaagag aagctgtctg ggcctgatga agtatttttt cggtgaagct ttttgcctga
     8641 tgaggagtat ggaagaagct gatcgggttt attccctgac taattatctg ttggcggcct
     8701 atctggaaaa gggcagaata gaatggaaag atattcagaa gctcggcata cagggttatt
     8761 cagaggtcat cgaaatgctg aatggctatg ctgacagact taagtcggac agtgaaaaat
     8821 ataaagatta ttacagggct aatgcattgg ccaagatata tcttgtaaga atgctttata
     8881 tcgataaaca ggagtcccta gatcaggaaa tcacaatata tcgaaaggca ttaatggatt
     8941 ctttggtcag ttttgccaat ctgaagcaaa ttttgggtga agtggagttc cttgactata
     9001 ttaccgggat gctccccaaa aaggataaaa agaaaatcga ggcattgatc aaggtaaagg
     9061 tggatgtggc aagcttattg acttagacgt tttctgcaac tacttctatc aacatcaact
     9121 gccaagcagc atctggcagc aaagggaaaa ttgaattaat ggtacggaaa accgactcaa
     9181 tacaagatca gtaatactat accgccttta gtcagtcaga gtctagaaac cttccttgat
     9241 aatgtggctt tcggcaattc ctagtgaagc gagatgacgc tggacagcat ccatcatagg
     9301 cggtggaccg cacaggtaat aataatgcct ttgcggatct gaattttttc tgattaattc
     9361 agcagtaata aatccgtgtt cgtagcccgg aatcttctca tcagataaaa cattgatgaa
     9421 tttatccccc agcaatttct caaaacgatc tgcatcaatg atgtcagctt tggttttgtt
     9481 ggcaaatatc agtttattgc cacctagttt gccttcagct tccaattgtt tcaggatggc
     9541 gatgaatgga gtgataccgg caccaccggc gatgaatata ccttcaccgg cataatggat
     9601 atctccatat acatcataaa gattgatttc atcacctgca tgaagttcac ggaggtagtt
     9661 ggtgacgccg ttgtgtgcct gataggtctt gatggtaaat tcgatagaat catccgaagg
     9721 cagggatgtg aaggtaaagg ttctgatctc tttatcccat ccttcctgat tgatcgatat
     9781 atcgacagcc tgccccggag tgtatatcag accttctgga cgttctgcca cgatgcgcag
     9841 tacatcgtgt gtaatagggg caattgattt tatttttact ttatgggcca ttttacttaa
     9901 tttttattta tacataggat aaaaactcat ttcttacgga atcctcctca aatcggccgg
     9961 aaagcattga tgtgatcgtt ttactattgg tgtccttgat gcctcttgaa gccacacaaa
    10021 gatgatcggc ttctatcatt actgctacat tgtcgtgttg caggatttcc ttcaacgcat
    10081 cggcaatctg gatggtaagc cgttcctgaa cctgcggctg ctttgcataa tactggacga
    10141 tacggttgag cttggatagt ccgatgatct tgtcatcagg aatataggcc acatgtgctc
    10201 ttccaatgat aggtacaaaa tggtgttcac aatacgaaaa caaagtaata tccttttcaa
    10261 tcaacatttt gttgtattga tacttattgt caaacagact aattacgggc ttatttgccg
    10321 ggttcaatcc gctgaatact tccttgacat acatttttgc gactctttct ggagtatcca
    10381 taaggctgtc atccgaaagg tccagcccta atgcatccat aatttgagca aaatggtatt
    10441 tgattttcct gattttctct tcatcggtca atccgttgaa ttctggtgaa tgctttgttt
    10501 cggatgagac ttctctatgg tttaatggaa ttgtcttttg catgacttac agttcgttta
    10561 caatattcat aacgttgttg accatagcgt cacaatatat caggttaaat gcaaacagct
    10621 cctcaccgat ataattcttt tcaatgattt cgcgcagcat gtttttagca cgattgaccc
    10681 ttaccttaac attcgcttcc gagatatcaa gtaattcggc cgtttcagct acattcaacc
    10741 cattgatttc acgaagggaa aaagccatcc tgtagtcgaa agggatttcg ctgagtgctt
    10801 tttcgataat ttctcccaat tccttgttat gaacaatcct ttctgtatca tggccggagt
    10861 gggaaaatag cggttttgag ttatcattta tatcttgcat tacttcattt ttatagctta
    10921 atttttcctt ttttcgaaaa caattattta acatgatacg gaaaagccag gtcttgaaat
    10981 tggctatgcc tttgtattgg ccaagatttt tgtaggcatc gatataggta tcctgcatta
    11041 gatcctgtgt atcatcgtgg ttgtagttgt aggcgcgccc caatttatag agaaaagggt
    11101 tgtaacgtct tactatgatt tcatataaag atttttctcc gttgaggatc cgtgatataa
    11161 tttctttttc attcaatatg tcgtgtatta ccattttacc ccgtaaaatt ttagtttgag
    11221 taacattaat atgtttattg tcaggtaaat attttattct cccgatatgt ttataagatt
    11281 agagtaaatg ggaaagaaaa aggttacaaa aaatgtaatt tattttttgt ggatttttgt
    11341 aacctcttct aaatgaataa tatggatctt tataatttat tctgatgcct ttaccagatt
    11401 agacagtgaa aatgcagcaa ttgccatgac cagatctcga actgccacat cgacatgatt
    11461 ccaaccggcg agtaaggaca atgctattat gatcagccag gccgcaacga ccagacctcc
    11521 tgtttttggt ttggtaaata ctataattcc ggcgatgatt tcgattacgc caactatcat
    11581 catgaaaacc gatccggaga agggaagtat ctcctcaaag ccggggttga tgtatgcagt
    11641 ccagtcggta agcatgttca ggaatttatc ggtacccgcc gcgataggta ggatggtgaa
    11701 agtgtatctc aataaatggg ttgttttctt tatgatggtt tcgttatatg ttgccatttt
    11761 gttgttactt atttaggtta gaaaaacacc ggggaaattc aaccattatc cccggccgga
    11821 tactagatgt gatcaattca ctaagagaaa gatttattcg aacaaggaag tcgtgaattt
    11881 tctctgaata gatagcgatt agaatttttc agggaattgt ttagcaatac cttcactgag
    11941 gtcgtctgcc atcataagca tatgttcatt tgcctcatcg aaaactttga ttgctttgct
    12001 gtaatccttc tgaagcaggt caacagcata ttctgtagtt tgatcaatgt gtgtcttcat
    12061 catgtgccgc atatcttcct gcttccagtt tggattagca ttagcaagga agtctgcgat
    12121 atcctgagca ttcttgtacc aatcattcaa ggcggttgtc aaagcttcgt tatttccttc
    12181 cttagcagca gtaagcaccg ggacggctcc tgtgatgtga tcggtcaata atttagtcaa
    12241 ctgaccacct gcatcctctc catagtatgg aatgatagcg gctccaatat ctttttggtt
    12301 ttgcaatagc ctgttcaaag ggccattcag cccgtccgga ttgttgaaga atgcgtccac
    12361 ggttgaatat gtccattgca tgtggtcact ccagagtttt ctcatattgg cattgagtac
    12421 cttatagctg gctgtagcag tcgggtcaat actttccttg ctacaactct ggaaagcaaa
    12481 tgtcataatg gctgctgcga taaggattcc tgcatatcta tttatgctgc ttaatttgaa
    12541 gttttttaat gcgtttttca tgtttataaa ttttaaatgt tattaatttt tgtactttga
    12601 ttttgggggt gctgatgttt agctattgat ttccccggcc gattcatgtc agcttcttgt
    12661 tttcagtgat tttagtgcac aaattatttt cgctgaaacc tgtaagtatg gtttagaatt
    12721 tttcgggaaa ctggagggcg attcctgagg ataactggtc ggcgaggtga agtagctggc
    12781 tgtaaatttc atcgtatgct tctatacttc cctggtaatt tccattcaaa tggttagttg
    12841 cttcgcttag tgtcagatcg atatactcct tcatatgttc cttcatatgg tgagatttcc
    12901 agttcatcgg attggcagta ctaagaaaca tcgctattgc atcgccattc tcatacaatg
    12961 agtcaatcat taggttcagg tcatcggtat ttcttgattt tgttgcggta aatacatttc
    13021 ttgctattac aatgtgttct ttcagtaaat atgtcagttt gtcgccggct tcttcggaat
    13081 agtatggctt gatggcttct ccaatatctt cctgattctt aaggagtctg gtgaaatcaa
    13141 ctgctgtgcc gggagcatct tctgcgatat caattattaa attccttgtc catgttgcat
    13201 gatcactcca taactttctc atgctattgc tgaagatttt atattttgta ctgtctaatg
    13261 ggttggtttc tatatcgtcc ttgtgacagc tctgtagcga taccagaagg atcaggaatg
    13321 ccgcaaaacg tttaatttga gttttgatta ttatagattc cttttgcttt gtccctttta
    13381 ttttatttga tgttattgtt gtttgcattt tattagagta ttatggctaa taagaggtta
    13441 ctatttattg aattttatta cggatttttt ctctgattta ttcgtatttt aatatccaat
    13501 atgtattcac gacagataat caaattgatg tatctcatta tttcagttct ttcctttcgt
    13561 aacttcttac attttgatta gagtattaag gataaaaaaa ggttacaggt attagaaaaa
    13621 tttttttgaa tgattacgta taagacaact tattttgttg aatagccttc aaaaaatgac
    13681 aaatttcatc cttaaaagga ccatgctcag gtcggtttgt atcaaaaggt gcaggttgac
    13741 aatcggtaaa gttttgtaga tcttataaaa ggtttcctgt ttcacagggg cgcctacgat
    13801 tgccagaaac gatcaatata ttcccatctc aaccgatcat ggatattttg gaattaatgg
    13861 ctggctgtct ccgaatattc aatatcagaa gtggaaacaa atgccatcca cattgtggta
    13921 aaactaaata ccttactttt atattactaa aatagttttt aaaaattctt ttctttttga
    13981 atcttcaaat catttcttta tcttcgttta cttaaatcac ctaaaatgaa tagaagaact
    14041 ttagtaagtt tcgactgggc attaaaacgc ctccttcgta ataaggccaa tttcaacatc
    14101 ttaagcgggt tcctttcaga attactcttt gacgatatat gtgtcgaaga gatattggag
    14161 tccgaaggca atcaagattc tgaccgggat aaattcaaca ggcttgacct tctgtgccca
    14221 aaccaacgta atgaatttat tatcatcgaa gttcaatatc acgatgagtc agactacttc
    14281 catcgttgtc tgtatggcac ctccaaggtc atcacggaat acatcaacaa aggagaacca
    14341 tatcatcagg tcaaaaaagt atattcggtt aatattctgt atttcgacct gggacacggc
    14401 gacgattata tttatgtcgg aaaaaccaaa ttcaccggat tacataacaa gtccaaccta
    14461 actttatcgc ccacccaaca aaaaaaattt gacaaatcag agccatttga aatttttcca
    14521 gaatatatat tgttgaaaat aaataacatt tgataatgtc tccagaaata cgcttgatga
    14581 atggatttat ttcttaaaaa actctgaact tccgcataag tttcatgcta aaggcttatc
    14641 agaagtatca gaaacactga aatacgacaa aatggataaa gtttcaaaac aacaatatga
    14701 ccaatatctc aaaaatatga gaatctctca ggatgctttg gataccgctt tggagaaagg
    14761 tttaatcaaa ggtcgcctgc aggaaaaaat cgatattgtg ctgaattgcc tttctaatgg
    14821 catagacatt cacctgatag ctaatataac caacctttct gaagaggaag tcgaaaaaat
    14881 aatcagggag
//
LOCUS       DAOQMC010000027        14889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1466_length_14889_cov_5.611635, whole genome shotgun sequence.
ACCESSION   DAOQMC010000027 DAOQMC010000000
VERSION     DAOQMC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1466_length_14889_cov_5.611635"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="pstA"
                     /locus_tag="PKV31_02030"
     CDS             <1..522
                     /gene="pstA"
                     /locus_tag="PKV31_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNB91660.1"
                     /translation="ASIPSIVFGLFGMALFVNKLKFGDSILAGSLTLGLLALPLIIRT
                     TEEALKAVPDAYRSGSLALGATKLQTIFRVVLPAALPNIFTGLILSIGRVSGETAPIL
                     FTVAAYFLPKLPSGIFDQAMTLPYHLYVLATSGTDLEASRPMAYGTAFVLIVIVLIMN
                     LIASFLRRRLERK"
     gene            549..1295
                     /gene="pstB"
                     /locus_tag="PKV31_02035"
     CDS             549..1295
                     /gene="pstB"
                     /locus_tag="PKV31_02035"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNB91661.1"
                     /translation="MIEAKNINFYYGSFHALKNISMQIKANTVTAFIGPSGCGKSTFL
                     RLFNRMNDLIEHTRLEGICSVNGRNIYDKSVNVDELRKKVGMVFQKPNPFPKSIFENV
                     AYGLRVNHIGDKKYIAQRVEESLKSAALWDEVKDKLHQSAFQLSGGQQQRLCIARTLA
                     IEPSVILMDEPASALDPISTSKIEELIHELKSKYTIVIVTHNMQQAARVSDTTGFFML
                     GELIEYSDTKKMFLNPEKEQTQNYITGKFG"
     gene            1516..2187
                     /gene="phoU"
                     /locus_tag="PKV31_02040"
     CDS             1516..2187
                     /gene="phoU"
                     /locus_tag="PKV31_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNB91662.1"
                     /translation="MKHNEKEIQLLKDEINQMWRLVISQMEKAKQAFLNNDAELALEI
                     ISLEKRVNAFELKIESNCENYIALYSPVAIDLRLVLSIMKISIALERIADYADGIAHH
                     VIDRDCDSLDHKVIEELELERMFDILLNMMTDSYAAINAENTASSGKILARDAEVDDI
                     YELAFSRLGEILKNHPDQIPCGLKLILLIRKMERIGDHCSNIVEDIVFYIEARVLKHS
                     GDRAE"
     gene            2221..2910
                     /locus_tag="PKV31_02045"
     CDS             2221..2910
                     /locus_tag="PKV31_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB91663.1"
                     /translation="MPQRILLVDDDRNISEILEFNLRNEGFEVNAVYSAEEALKLQIT
                     EYHIILLDVMMGGMSGYKLAEKLRNDNYSVPIIFLTARDTENDMLTGFSVGGDDYIPK
                     PFSIKEVIARVKAVLRRSRVESGTDPKSDKLKIGPITIDLEVKEVMIDHERISLTKTE
                     FELLVLLASKPDSVFSRERILRNIWNETPYISERTVDVHLARLRKKLGIYGAAISNRP
                     GYGYRFNTEAL"
     gene            2907..4610
                     /locus_tag="PKV31_02050"
     CDS             2907..4610
                     /locus_tag="PKV31_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB91664.1"
                     /translation="MKLTFRHRLFLYFAIIFTLFSIGIAIFEHSRETAFKTEVLQERL
                     MIYADVIHYSLSAQPEQFSNEVTQLISLWPADLRITIIDLDGRVLYDNAFTNVTLMSN
                     HLDRKEIHQAANNQFGTDIRKSASNKHPYLYFAKKYENRFVRVALPYDIQLKHLLKAD
                     NLFLYFLLGLFLVSLLFIHHITTRISNSIKKLHDLALHPTTSEIHFNEDELGAIGNKL
                     LDNFNLLETHKKNLQLEKQKLLQHIQISEEGICFMSSDHEVEFYNGLFLQYLNQLAEE
                     PHSDIQAFFQEPVFAPLLDFLNSGQSNYFETQIKRQGKIFSLRANIFVDKSFEIILTN
                     VTRQEKTKKFKQEMTGNIAHELRTPVTSIRAYLETVIEQQLPEDKKQYFILQAYRQTI
                     TLSEIIKDMGIIAKLEEAPGAFEFEPVNITRLLETVKAEESESLKEKNIRMGWNIPDK
                     LEIKGNNSLLKAIFRNLVENTIRYAGENIDIHVNLLSEDEENYYFTYYDTGVGVANED
                     HLNRIFERFYRIQEGRTRDTGGSGLGLAIVKNAVLFHKGTIAAKNRKTGGLEFLFHFH
                     K"
     gene            5196..5738
                     /locus_tag="PKV31_02055"
     CDS             5196..5738
                     /locus_tag="PKV31_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB91665.1"
                     /translation="MTDRLVSISGLINGYAPLIFTKESPISGLFYRIKYGNRPDLAEK
                     SGTFLGHSYKTISDRATDIDVITFVPIHPKKEAKRGYNQSRLIAQSVGKELDIPVKPF
                     LSRISNIDTQTHYGRQDRLNNQENTIKCDPAIRSFHHVMIIDDILTTGATLETCTDAI
                     RETAPGIRVSVATLAITDNW"
     gene            5889..6455
                     /locus_tag="PKV31_02060"
     CDS             5889..6455
                     /locus_tag="PKV31_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB91666.1"
                     /translation="MDIKKFWDEGLTYDEYIALSQKRLDHPANEEEAEKREYYELALQ
                     RMSRMTKRYVPDAGQVEAFAGKNFDGKVLIITEPWCGDASQAVPVANKFFEKNGVRIT
                     LRDQEPSLIDDFLTDGAKSIPIILFIDDNWKVVAQWGPRPAFGYELFLKYKANPEEYT
                     KEQFHNDLQVYYAKNKGYDSIEEILDKL"
     gene            6534..8150
                     /locus_tag="PKV31_02065"
     CDS             6534..8150
                     /locus_tag="PKV31_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNB91667.1"
                     /translation="MNLLLRLCFLFGLVALVACARPVLPDGGPKDTTPPQLIVEKSTP
                     NEQVHFEKKDLAFTFNEFITLDKADQQVLISPPLTMRPKISVKDKSVLFRFDEKEELR
                     PNTTYIINFGSSIKDYTEGNIVHDFQYVFSTGDNIDSLQLSANIKDAETGKPADNTVL
                     MLYRDLSDSVVYKILPDYAGRTDKDGSVTLRYMQPGLYQAVALNDENFNYKYDLTKEK
                     AGFLRDTILLPHHGKPLDMQIFGRDQPVRVTAVDSASNQRIRFIFEPSTAGIKLYSLM
                     EEPNNKIITGLKYIDVYYGDTTRSINAILMRPGFEADTIRHKLSGKVAARKPKVSQES
                     ADIKAPPHVPGEPVRIRFNVLLAAVDTSTMTLMTLKSKQAVPYLATIDPSDNNYMLLL
                     CNWMTDSTYLLNLQQGAIRDVLGNVNDSLEIQFKITDPSTLSALNIRIDSLQQDFDYI
                     IRLAKGNDIIRQWTISGKDSFKAEVLNLPAEAYDIHIMEDINKNGRIDKGNFDKKEDP
                     EKIYTRKLEQLRQNWTVDIEVNMNDFKNIR"
     gene            8545..8868
                     /locus_tag="PKV31_02070"
     CDS             8545..8868
                     /locus_tag="PKV31_02070"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNB91668.1"
                     /translation="MSSYFENVYALVRLIPRGRVTTFGAIANALGLGSARMVGWALNQ
                     LSGSDSSVPAHRVVNRKGMLSGQLHFDPGYSMAQRLIDEGVQVENDIVQDFKELFWDP
                     ALLAE"
     gene            complement(9006..9392)
                     /locus_tag="PKV31_02075"
     CDS             complement(9006..9392)
                     /locus_tag="PKV31_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91669.1"
                     /translation="MMMPATLGIQKAIFALLCLSFLLPACNKSNDIGDGNPPSGCLIT
                     LYEKDRFKGNAIILQGPANYLDLKAIPGSSKNWEKVAQSFEATGKAVVVFWKEPDLRG
                     DSISYPAASMISQLPFAPGSLKIKCD"
     gene            9743..9907
                     /locus_tag="PKV31_02080"
     CDS             9743..9907
                     /locus_tag="PKV31_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91670.1"
                     /translation="MKWFVLIAVIYIVFRVYRIGLINGSSSEKEKINRPGKSEAKDSN
                     IEYSDYEEIK"
     gene            9907..10611
                     /locus_tag="PKV31_02085"
     CDS             9907..10611
                     /locus_tag="PKV31_02085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB91671.1"
                     /translation="MQFSEQVLAKNHQYCIINKPAGQPVQPDKTGDISTMDMCKKYFH
                     HDVYLVHRIDRPVSGLVLFAKNTRSAAWLSELFRSKELDKTYLAIVENEPPHTSGSLV
                     SRIIEKKTGGNKSRIGEDNSANKGREAITKYEVIGRTDKYTLLAIKPVTGRKHQIRAQ
                     LAEAGCPIQGDVKYGARRGNKDRSIGLHAWRLSFTHPITGEIANFEAPLPNTNLWQAC
                     AEILKGESWQTPTEQK"
     gene            10584..11624
                     /gene="thiL"
                     /locus_tag="PKV31_02090"
     CDS             10584..11624
                     /gene="thiL"
                     /locus_tag="PKV31_02090"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNB91672.1"
                     /translation="MADTNRTEINTLGEFELINRLVSKFETVNQSTIRSVGDDGAVMD
                     YGPDKQVVVSTDLLIENIHFDLMYSPLKHLGYKSVVVNLSDIYAMNATPEQITVSIAV
                     SNRFSLESLEELYEGIHAACRHYNVDLVGGDTSSSNKGLIISVTAIGQGAKEDIVYRN
                     TAQPGDLICVSGDLGGAFIGMQILEREKALYLDNPGIKPELADYAYVVGRQLKPEARG
                     DVIKTLKAAGIKPTAMIDVSDGLASEIIHICSQSNVGAVIQEAKVPIHSMTEELAVKF
                     RMDPITCALNGGEDYELLFTIDPEDMDKIQFLDDVYIIGNILTEKDGIILETSGGNFH
                     RLKAQGWQHFNQ"
     gene            complement(11905..12390)
                     /locus_tag="PKV31_02095"
     CDS             complement(11905..12390)
                     /locus_tag="PKV31_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91673.1"
                     /translation="MKNLLLSMVILGFLVLGCRNSPSSSSDGGPVAISLPETEVDSVQ
                     VILDGNYCYRYMIHRDTYDIHLTLSQGQVRGEMNFDKQEDQNTSGSIEGYIKEDVMNL
                     KYRHRIDGTPKVRELSFRIRGNLLISAEGEELTEGDSTFFRDPASVRYEGLVYSRIDC
                     P"
     gene            13626..>14889
                     /locus_tag="PKV31_02100"
     CDS             13626..>14889
                     /locus_tag="PKV31_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91674.1"
                     /translation="MSRFQTFSASLFPGEVMILSRHSGMSDPEKLDILKRLLHSAGNP
                     EETFEVKPGTDKRKLSYVKTWCIDKLKAVDVDVRLSRLLQWEQQILLDNIASDEEKTF
                     LQTIRKTNASDFNFIKCYEIAHRYRHYLQVRFRKKEVATVVDFLDTFHRDYEKSKQLS
                     DEIHVATRDILILYEKKQELNTRWIGILSDIFFDEGNDGYTRILAWIRLVLIANSSND
                     YSFLEEHFKLFEAKLLDGTFYSKRIMANFYSQYLLFYANRQDFKKAAYYGRLSIKLKN
                     NDYLYYANNLVAVLLRDKNTAEALDILKSTATIASESTNFHNRLTHISYFIRALTDSG
                     KAEHAESRGFVFYNLFKKEIFNYRWYLFFSVWLRAMAYCGKFAGLLKMYKLYPGIGAE
                     EMRQNKRQGGFPFITFMSHVARYRLNEIS"
BASE COUNT         4609 a         3190 c         3106 g         3984 t
ORIGIN      
        1 gcaagcatac cctctattgt attcgggcta ttcggaatgg ccttatttgt gaataagctt
       61 aagtttggcg actctatatt ggcgggttct ttaaccctgg gtttgctggc cttgccctta
      121 atcatccgca ccacggaaga ggcattgaag gccgtacctg atgcgtatcg tagcgggagc
      181 ctggcccttg gagccaccaa attacagacc atctttcggg tagtattacc tgccgcgtta
      241 cccaacatat ttaccggatt gattctttcg atcggacggg tatcgggtga gacagccccc
      301 attctattta ccgtggcagc atactttctg ccaaaacttc cttccggtat ctttgatcaa
      361 gccatgacgc ttccttacca cctgtatgtg ctggccacaa gcggtacaga tctcgaagca
      421 tccaggccaa tggcctacgg tacggctttt gtccttatcg tcatcgtact gataatgaac
      481 ctgatcgcct catttttaag gaggaggctg gaaaggaaat aaatgtttta taaaaatcaa
      541 acatgaaaat gatagaagca aaaaatatca acttttacta tggcagcttt catgcgctga
      601 aaaatatcag tatgcagatt aaggccaata cggttactgc attcatcggt ccatcaggat
      661 gtggcaaatc aaccttcctt cgcctgttta accggatgaa cgacctgatc gagcacacca
      721 gactggaagg tatatgtagt gtcaacggtc gtaatatcta cgataagagt gtcaatgtcg
      781 atgagctgag aaaaaaagtt ggtatggtat ttcaaaaacc caatcccttc ccgaagtcca
      841 tattcgaaaa tgtagcttac gggcttcgtg tcaatcatat cggtgataaa aaatatattg
      901 ctcaaagagt cgaggaatcc ttaaaatcag ccgccctctg ggatgaagtc aaggataaat
      961 tacaccagtc ggcatttcaa ctctcagggg gccagcaaca acggctctgc attgccagga
     1021 cactggccat tgagccgtca gtcatcctga tggacgaacc ggcatcagca ctagacccca
     1081 tttccacctc caagatcgaa gaactaatcc atgaattgaa gtcaaagtac accatcgtca
     1141 tcgtaaccca caacatgcag caggcagcaa gagtgagcga tacaacggga ttcttcatgc
     1201 tgggtgaatt gattgaatac agtgatacta agaaaatgtt tctcaatccg gagaaggaac
     1261 aaacacagaa ttacattacg gggaagtttg gttaaaagat tttttgccaa cagagaatgc
     1321 tgaatgatgc agaaatccat cacagattta gtagtaaaag gattaatagg catcaaaaag
     1381 gatagcaaaa agtttaatga aagaaaattg ataataatca taaataatct cattgccaga
     1441 taaaacattt acctcggaag atggcgtcac gaagaatgat aaaaaaccga aggttgtaac
     1501 aataatcaaa gtaaaatgaa acacaacgaa aaggaaattc aactattaaa ggatgaaatc
     1561 aaccaaatgt ggcgattggt catatctcaa atggaaaaag cgaaacaggc ttttttgaat
     1621 aatgatgcag aactcgccct ggaaattata agtctggaaa agagggtgaa tgctttcgag
     1681 ttgaaaatag aaagtaattg cgaaaactat atcgctctgt acagtcctgt agcaatcgat
     1741 ctgagacttg tattgtccat catgaaaatc agcattgccc tagaaaggat tgccgactat
     1801 gcagacggaa ttgcccacca tgtaattgac cgggattgtg actcactgga tcataaagta
     1861 atcgaggagc tggaactgga aagaatgttt gatatcctgc tcaatatgat gactgatagc
     1921 tatgcggcca tcaatgctga aaatacagca tcttccggca agatattggc acgtgatgct
     1981 gaagtggacg acatctacga actggctttc agccggcttg gtgaaatact gaagaatcac
     2041 ccagaccaga ttccgtgtgg attgaaactg atcctgttga ttcgcaaaat ggaaagaata
     2101 ggtgatcatt gcagcaacat tgttgaggac atagttttct atatcgaggc aagggtgttg
     2161 aaacacagtg gagacagagc ggaataattt gctaattttg ctgcaaatag cttgaataca
     2221 atgcctcaga ggatattgct ggtggatgat gaccggaata tttcagaaat cctggaattc
     2281 aaccttcgaa atgaaggatt cgaggtaaat gctgtatatt ctgcggaaga agccctgaag
     2341 cttcagatta ctgaatatca cattattctg cttgatgtaa tgatgggcgg aatgtccggc
     2401 tacaagctgg cggagaaatt gcgaaacgat aactattcgg tcccaatcat attccttacg
     2461 gccagggaca ccgaaaatga tatgctgacg ggtttttcag tcggcgggga cgattatatc
     2521 cctaagccat tctcgatcaa ggaagtcatt gcaagggtaa aagcagtatt gaggcgtagc
     2581 cgggtagaat ccgggacgga cccgaagtcc gacaaactca aaatagggcc aataaccatc
     2641 gaccttgaag tcaaggaagt gatgatcgac catgaaagaa tctcccttac aaaaaccgaa
     2701 tttgagttgc tcgttttgct cgcatccaag cctgacagcg ttttttcaag agagcgcatc
     2761 ctccgcaata tatggaacga gacaccgtat atctccgaac gtactgtgga tgtacacctg
     2821 gcacggttga gaaaaaaact ggggatctat ggagcagcaa taagcaatcg accgggctat
     2881 gggtacagat ttaacacgga agctttatga aactcacttt tcgccatcgg ctattcctgt
     2941 attttgccat tatattcact ttattttcca ttggcatagc catatttgaa cattccaggg
     3001 agacagcatt caaaacagaa gtgctgcagg agagattgat gatatatgcc gatgtcatcc
     3061 attactctct ttcagcccag ccagagcagt tttcaaatga ggtgacccaa ttaatttcct
     3121 tatggccggc tgacctaagg ataaccatca ttgacctgga tggcagggtg ctttacgaca
     3181 atgcatttac taatgttacc ttaatgagta atcacctgga tcgaaaagaa attcaccagg
     3241 ccgctaacaa tcaatttggt acagacatcc ggaaatcggc atccaataag cacccttatc
     3301 tttattttgc caaaaaatat gaaaatcgct ttgtgagggt cgccctgcct tatgacattc
     3361 aattgaagca tcttttgaag gcggataatt tattccttta tttcctgctt ggcttatttc
     3421 ttgtttcttt attgtttatc caccacatca ccacccgaat tagtaattca atcaaaaaat
     3481 tgcatgacct ggccttacac cctacaacaa gcgaaattca cttcaatgaa gacgaattag
     3541 gagcaattgg taataagctg ctggataatt tcaatcttct ggagacccat aaaaaaaacc
     3601 tacaacttga aaaacagaaa ttgctccaac acatacagat ttcggaagaa ggcatttgct
     3661 tcatgtcttc cgatcacgaa gtcgaattct acaacggcct cttccttcag tatctcaatc
     3721 agcttgccga agagccacat agtgacatcc aggcattttt tcaggagcca gtctttgccc
     3781 cactgcttga tttcctgaat agcggacaat caaattattt tgaaacccaa ataaaaagac
     3841 agggaaaaat attctcacta cgggcgaaca tatttgtgga caagagtttt gaaataatat
     3901 tgaccaatgt aacccgtcag gaaaaaacaa agaagtttaa gcaggaaatg accggcaata
     3961 tcgctcatga gctccgtaca ccggtcacca gcatccgggc atatctggaa acggtcatag
     4021 aacaacagtt acctgaagat aaaaaacaat attttatttt acaggcttat cgccaaacca
     4081 tcaccttgtc agaaattata aaagatatgg gcatcatcgc caaactggaa gaagcacccg
     4141 gggcctttga gtttgaaccg gtgaacatca cccgactatt agaaacagtc aaagccgagg
     4201 agtcagaatc gttgaaggaa aaaaatatcc ggatgggctg gaatattccc gacaagctcg
     4261 agatcaaggg caacaatagc ctgctaaagg ctatatttag aaaccttgtc gaaaacacta
     4321 ttcgctatgc cggagaaaat attgacatcc acgtcaatct gcttagtgaa gacgaagaaa
     4381 attactactt cacttattat gatacaggag tcggagtcgc taatgaagat catctcaacc
     4441 gaatcttcga gcgtttttac aggattcagg agggcagaac aagagacacc ggcggaagcg
     4501 gcctggggct tgccatagtg aagaatgccg tactatttca caagggaact attgccgcca
     4561 aaaacaggaa gacaggcggt cttgaatttc tctttcattt ccataaataa attaaatcta
     4621 ttgactgcaa gataacatga ctgtgcccat agtcttcttc aaaaaagatg aaaccggaca
     4681 cctgtcattc gctgatttgt acaggcaatg acaaagactg aaggggaaag gatgctatgt
     4741 ccacacgttt tgctgaattg acatacaatt atcccttttc gtattacaat ttgcacaaat
     4801 aataaataca agtatagcca aagaataata cctctgtcaa atataccttg taccatgctg
     4861 acaaaataat aggataatgg gtcaaaagta tataagacaa atatcatcaa ttatttatac
     4921 acgccccaca tatcttccgg gccagggaaa agtacaattt gtaacaaaca atttttaccg
     4981 ggatttttgg caatatttgt gggtttggat tatctttgtt attaaatcca aactcctatt
     5041 acccgatgaa tttctttaga taccttgccg aagtttttta cccttcaaca tgtattcact
     5101 gtagtcgtgc cacatccggt caagatgatt tgttttgtac agaatgtctt acaaaaactg
     5161 tattctccgg ctttgttagc gccgtaaaga atgaaatgac ggacagattg gtcagtatca
     5221 gtggtttgat taatggatat gctcccttga ttttcacaaa agaatcgcct atttccggtc
     5281 tgttttaccg gattaaatac ggtaacagac cggacctcgc agaaaaatcc ggcacttttc
     5341 taggtcattc ctacaagact atctccgaca gggcaacaga tatagatgtc atcacttttg
     5401 tacccattca cccaaaaaaa gaggcaaagc gcggctataa ccaaagccga ctcatcgctc
     5461 aaagtgtggg caaagaattg gatattcctg tcaagccctt tctaagccgc atcagcaata
     5521 tcgataccca gacccattat ggccgacagg atagattgaa taatcaggaa aacacgataa
     5581 agtgtgatcc tgccatacga tcatttcacc atgtgatgat catcgacgat atccttacta
     5641 ccggagccac tttggaaacc tgcactgatg ccatccgcga aaccgccccg ggaatcaggg
     5701 tttctgtggc aacactggca atcactgata actggtaaac aggaagactc ctgaaaaaaa
     5761 atttgccggc gtaaaaagat gaacatttgt tcgttatctt gtgttattca cagtcaacaa
     5821 caagacatta ttggattgga ttttccaatt tatagggtct gaatcgacat gaacaatcaa
     5881 acaatactat ggatattaag aaattttggg acgaaggctt gacctatgat gaatatattg
     5941 cgctgtcgca aaaaaggctg gatcatcctg caaatgagga ggaagccgaa aagcgtgaat
     6001 attatgagtt ggcactgcaa cgcatgagcc gaatgacaaa gcgctatgtt ccggatgccg
     6061 gacaggtaga ggctttcgcc ggaaaaaact ttgacggtaa ggtattgatt attaccgagc
     6121 cctggtgtgg cgatgccagt caggcagtgc ccgtcgctaa caaattcttt gagaaaaacg
     6181 gagtccggat tactttgcgt gatcaggaac cttccctcat cgacgacttt ctgacggacg
     6241 gagcaaaatc aattcccatc atcctgttta tagatgacaa ttggaaagtt gtagctcaat
     6301 gggggccccg tccagccttt ggatatgaac ttttcttgaa atacaaggcc aatccggaag
     6361 aatacaccaa ggagcaattc cacaatgacc ttcaggtata ttatgccaaa aacaaaggct
     6421 acgattcaat tgaagagatc cttgacaaac tctgacaata cgtggcctga agtttcaatt
     6481 ctgatgtttg acagcgagtc agaatcaact atatttgctc tttattcaat ccgatgaatc
     6541 tgttgttgcg tttatgcttt ctatttggcc tggtagcttt ggtggcctgt gcaagacctg
     6601 tgcttcccga tggaggacca aaagacacaa ccccgcctca attaattgta gaaaaaagca
     6661 cacccaatga acaggttcac ttcgaaaaaa aggatcttgc ttttactttc aatgaattta
     6721 taacattgga caaagctgac cagcaggtac tgatcagccc tccgttgacg atgagaccaa
     6781 aaatctctgt caaggacaaa tccgtattgt ttcgatttga tgaaaaggaa gagttgcgtc
     6841 ccaacaccac gtatatcatc aactttggca gttccattaa ggattatacc gagggaaata
     6901 ttgttcatga tttccaatat gtgttcagca ccggtgacaa cattgactcc ttacagcttt
     6961 cggcgaatat caaggatgca gaaacaggca agcctgccga caatacagtg ctgatgctgt
     7021 atagagacct gtcggacagt gtggtgtata agatattacc ggattatgcc gggcgcacag
     7081 ataaagatgg aagtgtgacc ctgcgatata tgcagccggg cctataccag gcggtagccc
     7141 tcaatgatga aaattttaat tacaaatacg atctgaccaa ggaaaaagcc ggatttttac
     7201 gagatactat cctgttgccc catcatggca aacccttgga catgcaaata ttcgggagag
     7261 atcaacctgt ccgggttact gctgtagata gtgcatccaa ccagcgtatc cgatttatct
     7321 tcgagccatc aacagccggc atcaagctgt attcactcat ggaagagcca aacaataaga
     7381 tcataaccgg cctcaagtat atcgatgtat attatggaga taccacccgc tcaatcaatg
     7441 ctatattaat gcgccccgga tttgaagcgg acaccatcag gcataagctt tcaggtaagg
     7501 tggctgcacg aaaacctaaa gtctctcagg aatccgccga tataaaagca ccaccgcatg
     7561 tgcccggaga acctgtcaga attcgtttta atgtcttgtt ggctgctgtc gatacatcca
     7621 caatgacttt gatgacttta aaaagtaagc aagcagtgcc ttaccttgca accatcgatc
     7681 cgtctgacaa caattacatg ttgctccttt gcaattggat gaccgactcc acttatcttt
     7741 tgaaccttca gcaaggcgct atccgggatg tcctgggcaa tgtcaatgac tccttggaga
     7801 ttcaattcaa aataaccgat ccatctaccc ttagcgccct taatatcaga atagacagcc
     7861 tgcaacagga ctttgattat atcatccggc tggccaaagg aaatgacatc atcagacaat
     7921 ggacaatttc gggcaaagat tcctttaagg cagaagtatt aaatctcccg gcagaagcct
     7981 atgatatcca catcatggag gacatcaata aaaacggaag aatcgacaag ggtaattttg
     8041 acaaaaagga agatccggag aaaatttata ccagaaagct ggagcaacta cgtcaaaact
     8101 ggaccgttga tatcgaagtc aacatgaatg atttcaaaaa cattcgatag gaagcaagcg
     8161 atattgttga aagtcagtcg atcaggacaa caaaatagcc gccagattgg ctgaaagaga
     8221 ttggttatgt aagggtaaat cgaagtaggt aggaaaataa tagtgtccga gtgagagggc
     8281 acaaggaatt taaaagatga taatctaaat tgctgcttaa atccaagtca attttttaat
     8341 agcctccctt ctcaagcttg ccagatcaaa taagtcggtt attatttatc tacctaattc
     8401 catttttttc gggcccaact tcacacccga aatgaaaaac aacagaagct aatgctataa
     8461 tgggaaaagt cagagcataa tatgtatcct ctgatgacca aaatttacag aactttattc
     8521 gatgatccca cttatttttg tgccatgtct tcatattttg aaaacgtgta tgctttggta
     8581 aggctaatac cacgaggcag agtgactaca ttcggtgcca ttgccaatgc gttaggactc
     8641 ggttctgcca ggatggtggg ctgggcgctc aatcaattat ccggaagcga ctcatcggtt
     8701 ccggcacatc gtgtagtcaa ccgcaaaggc atgttaagcg gtcagctgca cttcgacccc
     8761 gggtattcta tggctcaaag gctcatcgat gaaggggtgc aggtagaaaa tgacatcgtg
     8821 caggatttta aggaattgtt ctgggatccc gccctattgg cggaataaat gaatgcatgg
     8881 ttttaccatc actcttgcat tgcttcgaaa ggcgataagt gctatccttc aaaccatgaa
     8941 tttccaattg gtttttccag ggaccaataa gtcatcaact aaattaccaa aggaaaatta
     9001 attggctaat cgcacttaat cttcagactt cccggggcaa agggcagttg agatatcatc
     9061 gatgctgccg gataggaaat ggagtctccc cttaggtcgg gctccttcca gaatacaact
     9121 actgcctttc cggttgcctc aaaggattgg gctacctttt cccagttttt tgagcttccg
     9181 ggaatagcct tcaggtccag gtaatttgcc ggaccctgaa gaatgatggc attgccctta
     9241 aatctatctt tctcatacaa tgtgatcagg caacctgatg gcggattgcc atcccctata
     9301 tcgttggact tattacaggc aggaagcagg aaggacaggc acaacagggc aaaaatggct
     9361 ttttgaattc ctaaggtggc aggcatcatc ataaatcact catttaaatt gtgttgcaaa
     9421 catagtcaaa aagtgaaaaa tgaacattag tttgagaaaa actctccttt aacaattagc
     9481 attacaattg ctgctcgggc agattatggt atatggaatt catgttatac caagatgatt
     9541 tatataatag tccataagtt tttggtataa tgcagaaggt acattcagta ttttagtcgg
     9601 ccgattttcg tacaatcatg cagaaagcgc atcaaagcca acaatcccta taatccgtaa
     9661 tatacaaaga acaaggctca ctgtaagttt aatataaaag ttggccgatt ttttgtaatt
     9721 ttgcagtctt aatcattatg ctgtgaagtg gtttgtcttg attgccgtta tctacattgt
     9781 tttccgcgtt tatcgcattg gtttgatcaa tggttcctca agtgaaaagg agaaaatcaa
     9841 ccgacccggc aaatcagaag ctaaggacag taatattgaa tattcggact acgaagaaat
     9901 aaaataatgc aattcagcga acaggtactg gccaaaaatc accagtactg catcattaat
     9961 aagcctgcag gacaaccagt acagcctgac aaaactggtg acatcagtac gatggacatg
    10021 tgcaaaaaat actttcacca cgacgtatat cttgtccacc gtatcgatcg acctgtttca
    10081 ggattggttt tatttgccaa aaataccagg tcggcagcgt ggctaagcga gctcttccgt
    10141 tccaaagagt tggacaagac ctatcttgcc atcgtggaga atgaaccgcc tcatacatca
    10201 ggaagtcttg taagccgtat cattgaaaaa aaaaccgggg gtaataaatc aagaatcggc
    10261 gaagataatt cagccaataa ggggcgtgag gcaattacaa aatatgaagt aataggacga
    10321 acggacaaat acaccctctt ggccataaag cctgtgacag gacgaaaaca ccagatacgg
    10381 gcacaactcg ctgaggccgg ttgcccaatc cagggagatg tcaagtatgg agcacgacga
    10441 ggcaataagg atcggagtat tgggctccat gcatggaggc tttccttcac ccatccgatc
    10501 acaggagaaa ttgctaattt tgaggcaccg ctgcccaaca ccaatttatg gcaagcgtgt
    10561 gctgaaatat taaagggaga atcatggcag acaccaacag aacagaaata aatacccttg
    10621 gtgaatttga gctaatcaat cgtctggtct caaagtttga gaccgtcaat caatcaacta
    10681 tccgttctgt aggcgatgat ggtgcagtca tggattatgg acctgacaaa caggtagtcg
    10741 tcagtacaga tctattgatt gaaaacatac atttcgactt gatgtattca ccgttgaaac
    10801 atttggggta caagtccgtc gttgtcaatc tctcagacat ctatgccatg aacgccacac
    10861 ccgaacaaat tacggtgtcg attgcagtaa gtaaccgatt cagtctggaa agtcttgagg
    10921 agttatatga agggattcat gcggcgtgtc gacattacaa tgtggatttg gtcggaggag
    10981 atacttcctc atccaataaa ggacttatca tcagcgtaac tgccatcggt cagggggcaa
    11041 aggaagatat tgtatacaga aacacggctc agcccggtga cctgatctgt gtaagcggtg
    11101 atttgggagg agcgtttatc ggcatgcaga ttctggagcg tgaaaaggct ttatacctag
    11161 acaatccggg tatcaagccc gaactagcag attatgcata tgtcgtgggc cgtcaattaa
    11221 aaccggaagc aaggggcgat gtcattaaaa ccttgaaagc ggcaggtatc aagcctacgg
    11281 ctatgatcga cgtatccgac ggccttgctt cagagattat ccatatctgt agccagtcta
    11341 atgtcggtgc tgtgattcag gaggcaaaag tgccgataca ctctatgacg gaagagctcg
    11401 ctgtcaaatt tcggatggac cccattacct gtgcgttaaa tggcggtgaa gactatgaat
    11461 tactttttac gatagatccg gaggatatgg ataagataca attcctcgat gacgtatata
    11521 tcattgggaa tattctgacc gaaaaggatg gaatcatact ggaaacttct ggcggcaatt
    11581 tccatcgact aaaagcccag gggtggcagc atttcaatca gtgattctga ctgaaacctt
    11641 gaaaagaccc attcgaggca gcttggtaag ccaatcagtc actaatagga tgttttttag
    11701 ttgtcttcaa actatattaa aatatcttcg accgttagta tgcattcgca aaataattac
    11761 ccaatctgta caaaactgtt tgtctatttg ttacaaaccc ggaagcttta gttaagatat
    11821 aaaaactgct tttgtacatc ccggctatcg caaacggacc ttcatcatgt aacccaaaac
    11881 gggaatctcc attaatcaac cgactcaagg acaatcgatc ctggaatata ccaacccttc
    11941 ataacgtact gatgcaggat ctctgaaaaa agtcgaatcg ccttcggtca attcttcccc
    12001 ttcagcgctg ataagaaggt taccacggat cctaaaggac agctccctga ctttaggtgt
    12061 cccgtcaatc ctatgtcggt atttaaggtt catgacatct tccttgatat aaccttcaat
    12121 gctcccactt gtattttggt cttcctgttt atcgaaattc atctcccccc ttacttgacc
    12181 ctgtgacaag gtaagatgaa tgtcgtacgt gtctcggtgt atcatataac gatagcagta
    12241 attgccatcc agaattacct gtacactgtc cacttccgtt tccggaagac tgatggcaac
    12301 aggccctcca tctgaagacg aagaaggact attccggcat cccaatacca aaaaaccaag
    12361 tatcaccata gacaaaagta gattcttcat aaaaatatta ctttttgaaa ataaaaaata
    12421 ggtattgcta tctgcgaata taacgctaaa tctcacttta atacttcaat aaatattcag
    12481 aaaaaatagc ctcctaccat cctgatttac agcacattgt aaagaaaaga cggttggggc
    12541 gtggtttttc ctgaagtagg tagatgatta atagtcaccc tgccaataga tatgctcctc
    12601 ttcgggtgcc attacgtaaa ttctattcag gattgaccat attttttatt cgtccggggc
    12661 aaatggttca ctttaccttg gtcagcggtt attaaacatg tacatacttc tttttttcca
    12721 aaaaaattaa ccgaacaccc actactgtaa cgcactcctg tagtaggtag atgattaata
    12781 gtcaccttgc caatagaatt gctcctcttc gggtgccatc aggtaaattc tattcaggag
    12841 tgagcatatt ttttattcgt ccggggcaaa tggctcactt taccttggtc accggttatt
    12901 aaacatgtac atacttcttt tttttcaaaa aattaaccga acacccactg ctgcaacatt
    12961 ctcctgaagt aggtagatga ttaatagtca ccttgccaat agatatgctc ctcttcgagc
    13021 gccattaggt aaattctatt cagcattgag catatttttt attcgtccgc ggcaaatggt
    13081 acactatact ttggtcagcg gttattaaac atgtacatac ttcttttttt tcaaaaaaat
    13141 taaccgaaca ccaactactg caacactctc ctgaagtagg tagatgatta atagtcacct
    13201 tgccaataga attgctcctc ttcaggtgcc attacgtaaa ttctattcag gattgaccat
    13261 attttttatt cgtccggggc aaatggtaca ctttacctca gtcggcggtt attaaacatg
    13321 tacatacttc ttttttccaa aaaaattaac cgaacaccca ctactgtaac gctctcctga
    13381 agtaggtaga tgattaatag acagaaagaa agcgaggccg tacttttaat attccaaaca
    13441 gaaaaattat cttcatatag atgattgtag ttttccgact tcctccaaat cctttgctat
    13501 acgatccttt tcagctatta ctttcgtaca cacttcgatt tttccccaca aaacaacctc
    13561 tctcaaattt ttatttattg aaccactatc tatgtttttt catcctttca ccttattttc
    13621 ggcgtatgtc cagatttcaa acattttcag cttctctgtt cccgggtgag gtcatgattc
    13681 tcagcagaca ttcaggtatg tcagatccgg aaaaattaga catcctgaaa agattactcc
    13741 atagtgccgg caatccggaa gaaaccttcg aagtaaagcc gggtacagac aaacggaagc
    13801 taagctatgt taaaacctgg tgcatcgaca aactgaaagc cgtcgacgtg gatgtacgcc
    13861 taagtcgcct gctccaatgg gaacaacaaa tcctgctgga caatattgca agtgatgagg
    13921 aaaaaacctt tctacaaacc attagaaaaa ccaatgcttc ggatttcaat tttattaaat
    13981 gttatgagat tgcgcaccgt tatagacatt accttcaggt gcggttcagg aaaaaggagg
    14041 tcgctactgt ggttgatttt ctggacacat tccacagaga ttacgaaaaa agcaagcaac
    14101 tgagtgatga aattcatgtt gctacacggg atatcctgat tctatatgag aaaaaacagg
    14161 aacttaacac ccggtggatt ggcattctaa gcgatatttt cttcgatgag ggcaatgatg
    14221 gctatacgag gattctggca tggatcagac tggtgctgat cgccaacagc agcaatgact
    14281 attctttcct ggaagagcat ttcaagctgt ttgaagcaaa attgttggat ggcactttct
    14341 actcaaagcg tatcatggcc aatttctaca gccagtacct gcttttctat gccaataggc
    14401 aggattttaa aaaggcagcc tattatggtc gtttgtcgat taaactcaag aacaacgact
    14461 atctttatta tgccaataac ctggttgctg tattactgcg cgataaaaat accgctgaag
    14521 ctcttgacat tctcaaatcc accgcaacga ttgcctccga atcgaccaat tttcacaatc
    14581 ggcttaccca catctcttat ttcattcgcg cccttaccga ctccggaaag gctgaacatg
    14641 ctgagagccg tggcttcgtc ttctataatc ttttcaaaaa ggaaattttc aattaccgat
    14701 ggtacctgtt cttcagtgtt tggttaaggg caatggcgta ttgcggcaaa tttgcaggcc
    14761 tgttgaagat gtacaaactt tatccgggca taggtgcgga agaaatgcga cagaacaaac
    14821 gacaaggagg attccctttt attacgttca tgagccacgt tgcgcgctac cggctcaatg
    14881 aaatcagcc
//
LOCUS       DAOQMC010000028        14800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1479_length_14800_cov_4.773143, whole genome shotgun sequence.
ACCESSION   DAOQMC010000028 DAOQMC010000000
VERSION     DAOQMC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14800
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1479_length_14800_cov_4.773143"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKV31_02105"
     CDS             complement(<1..318)
                     /locus_tag="PKV31_02105"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB91675.1"
                     /translation="MSKTALITGITGQDGAYLAELLLSKGYMVHGIKRRSSLINTERI
                     DHLYQDPHESNVRMKLHYGDLTDSTNLIRIIQETQPDEIYNLAAMSHVHVSFETPEYT
                     ANAD"
     gene            complement(1081..1227)
                     /locus_tag="PKV31_02110"
     CDS             complement(1081..1227)
                     /locus_tag="PKV31_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91676.1"
                     /translation="MLFQNINYYSVLSKWDDARKILVHAGDFRQHRTAGEIIPWSDWG
                     IKAG"
     gene            complement(1497..2552)
                     /gene="wecB"
                     /locus_tag="PKV31_02115"
     CDS             complement(1497..2552)
                     /gene="wecB"
                     /locus_tag="PKV31_02115"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB91677.1"
                     /translation="MKIAPVIEAIEKAKAEGKNISYRLVHTGQHYDQKMSGDFFTQLG
                     IPQPHINLGAGGGTQAQQTAAIMQGYEKALMDDRPDLVMVVGDVTSTMACAITAKKMN
                     NIRVAHIEGGIRSGDWTMPEEINRIVTDSITDYFFTTSETANENLRKEGKTEDQIFFV
                     GNTMIDTLYRNMDRLRRPSLYDTFHLEAGNYIVMTLHRPANVDQEAGLVRLIRTIIDS
                     SRGIPLVFPVHPRTAGVLEHASLSAPNLHLVEPLGYLEFNYLVKHAKAVITDSGGITE
                     ETTVMGIPCMTLRDNTERPETCTIGTNELLGTDPAAIPPAMDILFSGKWKKGGIPELW
                     DGKTAERIVGEVLSFEF"
     gene            complement(2680..3822)
                     /locus_tag="PKV31_02120"
     CDS             complement(2680..3822)
                     /locus_tag="PKV31_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB91678.1"
                     /translation="MIQRKAKQELLTLANQFKAVVVIGPRQSGKTTLVKDAFPDKPYV
                     NFENPDVRLFASEDPRGFLANYQDGAIFDEVHRSPDMFSYLQEILDNQKEAGKYILTG
                     SNNFLLQENISQSLAGRVAYLQLLPLSIDEIKDIPQSTNQLMYNGGYPAIYDQLIPAT
                     KWYANYIQTYVERDVRQILNIGDLNKFEKLLRLCAGRIGQLLNMSSLAVEVGVDVKTV
                     DSWLSVLEAGFVLFRLQPYYENYNKRIVKMPKLYFYDIGLAWALVGINDPDQIALHPF
                     RGSFFENLVILEVKKQFLNRGETHNLYFWRDSNGSEIDLLIKQSGQIQPVEIKSGQTI
                     IQGYFKGLVFWNKITNTSGGTVIYDGIHRQNRSSGFSVVPLREMRF"
     gene            complement(3962..4306)
                     /locus_tag="PKV31_02125"
     CDS             complement(3962..4306)
                     /locus_tag="PKV31_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNB91679.1"
                     /translation="MKESIVKDRSFKFALGIIEIYKRLQEQNEFVLSKQLLRSGTSIG
                     ANINEALAGESRADFKHKMSIASKEARETKYWLELLEQSQLREISFKLLIQESQELIN
                     MLTSIVKTTKKT"
     gene            complement(4681..6282)
                     /locus_tag="PKV31_02130"
     CDS             complement(4681..6282)
                     /locus_tag="PKV31_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNB91680.1"
                     /translation="MKSFDYIIIGGGSAGCLLANRLSDNAQTEVCLLEAGPSDDSHFI
                     HNTNPVNMLYLLNSPKYNWKYYAEGNHKTGKRRFFWPRGKTLGGSSAVNAMIYTRGHP
                     SDFDRWAALGNEGWDYRSVLPYFLKSERQHRGGDAYHNDTGEMDVIDPNFHFPASTAF
                     IEACKQAGIPHNRDINGEQYEGVDFFQINQTPDGQRAHSAVSFLDPIRHRKNLTIITG
                     AHVNKVLMNDGTAYAVEYFDLKNARRLCTLNARREIILSAGIIGSPQILMLSGIGPAA
                     QLQQLGIEIVKDLPGVGENLQDHPDVILRYRDKSGTSLTTFPWPYMIRFMSRFYKKNS
                     NFIFTPTDAGGFIKSDPSLEVPDLQLQFASLRMQPHGAGLTPTLAGYVLHICHMRPNS
                     RGRIRLTSADPFAHPRIEANYFDDEMELEALVKGVKIARNILSQPAMQPFNKKEEVPG
                     ASCRTDEDIRRFILKKVESVYHTAGSCKMGIDNMAVVDPRLRVYGIRNLRVIDSSIMP
                     TITGSNIHAPTVMIAEKGAELVVKG"
     gene            complement(7188..8399)
                     /locus_tag="PKV31_02135"
     CDS             complement(7188..8399)
                     /locus_tag="PKV31_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB91681.1"
                     /translation="MRILFLTDNFPPEVNAPASRTYEHCIEWVRRGVEVTVITCVPNF
                     PQGKVFDNYKNKWRQKETMDGIRVIRVWSYISANEGFGKRILDYVSFGVTSFLHGLFV
                     KTDLIVATSPQFFTAVSGRWLSLCKRKPWVMEVRDLWPESIKAVSAMKSDNMIFSYLE
                     WVEKRLYKSAKKIIVVTDAFRDKIASRGIDKNKISVVKNGVLMEKFKPVEKNRQLLSQ
                     LGLEGKFIFGYLGTHGMAHKLDFIVECAAKITDEDIHFLFIGNGAQKENVRNLAVQLR
                     VKNCTFLDSVPKNEIADYISITDVALVNLMKSETFQTVIPSKIFENASMQKPILLGVE
                     GESKSIIEQYDAGICFEPENEAAFLDAVYRLKSDAQLYGKLQEGCKRLALDFDRRKLA
                     LRMLEIFNQVT"
     gene            complement(8396..9769)
                     /locus_tag="PKV31_02140"
     CDS             complement(8396..9769)
                     /locus_tag="PKV31_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNB91682.1"
                     /translation="MGGRKFRFINLEKDFGDKIDWNYEAFGKLWTYNLNYFEWLMQED
                     ISVEDGLKSIHEFINHYDEIKDGRESFPISLRLLNWIKFISENDIQDSRIDQVIHQDA
                     WRLYHHPEYHLLGNHLLENGFGLFFAGSYLNDDKLLGLAERILRSELEEQILPDGGHF
                     ELSPMYHQHMHFRVLDCIQLIHKNGHYSRQLLPLLTDKAEKMLGWLASMTFDDGTIPL
                     FNDSANNIAPTSIQLFTYAKELGLHVRKTELKESGYRRLKNDRLDCIIDVGNIGPDYI
                     PGHAHADSLSFELQVDGRPFIVDTGTSTYEVNEVRQQERSTSAHNTVVINDCNQSEVW
                     GGFRVARRVQVEVLCDEKNHIEAVVRGYSDKNISHTRRFTLESNSLIIEDEVKGSHAP
                     INNRCFFHFYPGVKPGYTSDISIITEGETSKKVMNFNYAPEFNKHIVSDCLEVKFDKK
                     LKVSISL"
     gene            complement(10001..12112)
                     /locus_tag="PKV31_02145"
     CDS             complement(10001..12112)
                     /locus_tag="PKV31_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNB91683.1"
                     /translation="MKQIVQDLKSGQTILEEVPVPQVRPGCLLIKTHCSLVSLGTERM
                     LVEFGKAGWISKARQQPEKVMQVIQKIKTDGLRPTMDAVFRKLGEPLPLGYCNAGEVI
                     AIGKGVLGYTIGDRVISNGNHAEIVCVPENLTAKIPDEVSYEEASFTVIGAIALQGIR
                     LINPTFGETIVVTGLGLIGLLAAQLLKANGCKVIGLDFDGRKVDLAKSFGIEAIKVSS
                     EMDVPAAIMSHTNGIGADGVLITASTKSDEVISQAAQMCRKRGRIVLVGVIGLDIKRS
                     DFYEKELSFQVSCSYGPGRYDEHYEQKGLDYPIGFVRWTEQRNFEAVLSAIVGGGLKV
                     NELITERVILDEYNAIYGDMSKSGSIASIIQYPGDVDVSKTSVSVVSHDFSGGSAQIG
                     IIGAGNFTGAMIVPVLKELKADMKYIASAKGLSGTTLAKKYGIAHSTSRYQDILEDKD
                     VRAVLITTRHSAHAGQVIEALQHGKDVFVEKPLALNMEELDAVKDAYTSCGKSLTVGF
                     NRRFSPFSIAARECLGDGPIQVIATMNAGFIPANSWVQDMESGGGRIIGEACHYIDLI
                     TYFTGSRVEKVIMNAIGTHPSVSTDNASILLKYEDGSTGVINYFATGNKAYPKERIEI
                     FSQGKNILIDNFRKASFYGYKRSDMKKTQDKGHREQFSRWLQSVKNGGNPLIEFDDLY
                     NTSKAAILAVESLTEGRWMEL"
     gene            complement(12174..13328)
                     /locus_tag="PKV31_02150"
     CDS             complement(12174..13328)
                     /locus_tag="PKV31_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNB91684.1"
                     /translation="MNFELLVNTIQATHNQLQQSAIRSVNIHLTIRNWLIGFYIVEFE
                     QNGSDRATYGERLFAELAKTLNVKGLAETNLKLSRQFYLVYPQIAQIVSEQLQQIGFS
                     SIRHLSTDELQIHDNQTVKIRQTASDEFISTLPEGLQVPPNQLINSLSFSHFVLLLPI
                     EDPLKRTFYEIECMKGTWSVSELKRQINSLYFERSGMSTNPEKLSRLVNDKAHALVPT
                     DIIKSPFTFEFLGLKAKDVVYESDLEQALIDHLEEFLLELGYGFCFEAKQKRIIIGDR
                     YYHIDLVFYHRILKCHVLIDLKMKEVEHENIGQLKTYINYYKKNFMLKDDNPPVALLL
                     VTENNKELVEYAVADTDKDLFVSKYILELPSTEKLQEFISKELRALRDKL"
     gene            complement(13391..13633)
                     /locus_tag="PKV31_02155"
     CDS             complement(13391..13633)
                     /locus_tag="PKV31_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91685.1"
                     /translation="MKQIVQDFKSGQTILEEVPVPQVRPGCLLIKTHCILVSLGTERM
                     LVEFGKAGWISKARQQPEKMILYVNSTLSIDDFNLL"
     gene            complement(13630..14409)
                     /locus_tag="PKV31_02160"
     CDS             complement(13630..14409)
                     /locus_tag="PKV31_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HNB91686.1"
                     /translation="MLRPRITPCLLVHKKGLVKTVQFKDPKYVGDPINAVKIFNEKEV
                     DELMVLDIDATVEGRGPDFTMIKNLAIECRMPFCYGGGVTTVDQAKNIIKLGAEKVAL
                     SSAAINDTSLLRKMGEAVGSQSVVVVIDVKKKKGLLGKTTYEIYTKNGREKSGKKLEN
                     FLKELNDIGVGEIVINSIDRDGTMEGYDNTLVKLVRDAVDIPITILGGAGSHEDIKSL
                     IKDYKIIGAAAGSLFVFKGKYRAVLINYPNRIEKRNILNNQ"
     gene            complement(14402..>14800)
                     /gene="hisH"
                     /locus_tag="PKV31_02165"
     CDS             complement(14402..>14800)
                     /gene="hisH"
                     /locus_tag="PKV31_02165"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNB91687.1"
                     /translation="PVIGVCVGMQILAEGSEEGKLPGLGWVKGIVKKIDVNLLELKPK
                     IPHLGWNSIKVSKQSELFHSIDEEMGFYFLHSYYFECDNPGDIMSTTFYGKEFASSVN
                     HENIYGVQFHPEKSHQNGVTLLKNFANLNA"
BASE COUNT         4088 a         3339 c         2784 g         4589 t
ORIGIN      
        1 gtcggcgttg gctgtgtatt ccggcgtctc aaagctgacg tgaacgtggc tcattgcagc
       61 gaggttgtat atttcgtccg gctgggtctc ctggatgatg cggatgaggt tggtcgagtc
      121 ggtgaggtca ccgtagtgca gtttcatccg tacattggat tcgtgcggat cctgatacag
      181 gtggtctatg cgttcggtat tgatcaggga ggaacggcgt ttgatgccgt ggaccatgta
      241 gcctttggag agcaacagtt cggcgaggta agcaccgtcc tgtcctgtga tacctgtgat
      301 taatgcggtt tttgacattt ataaaatttt aatttaatct acgccttgca tattcagcgc
      361 aaaatttatg taatcgaaga taaggtttct tgtaacaaaa taaggggtgt tttacagatt
      421 tttgtaacaa aataaggggt gtttttgaag ttcttagttt tgagttctta gttcttcata
      481 gttttgagtt ttgagttctt agttcttagt taaattcaac tcaaaactca aagctttaaa
      541 cttaaaactt aataaggtgg cttctaatga agctttatat tccgattgac ttttttatcc
      601 tgatttttta aatacctctt tctattattt taatttttcc ggattgatag cgctgtaggc
      661 gcgacctttt ggtaaaattg tccatttatg taggagccaa gctccgtagg tgcggccttt
      721 tgttttattc tggctatttc ttgcaatatc ataacatagg gttttataag gtcgccccta
      781 cagagcttgt ttctacgcta tttacatcat cctaccataa ggtcgcttct accgaagctt
      841 tgcattccga ttgattttat aattcggaat ttaaatacca atttcgttaa tctaattttt
      901 tccggattga tagcgctgta ggcgcgacct tttggtagaa ttgtccattt atgtaggatc
      961 caagctccgt aggtgcgacc ttttgtttta ttcaatttac tgtttgtaat atcgtgaatt
     1021 ggttttttta gattaccttt atatggcgtg atcgcacata tattttatca tgtttccgtt
     1081 tcatccggcc ttaattcccc aatctgacca aggtataatc tctcctgccg ttcggtgttg
     1141 tctgaaatct ccggcatgta ccagaatttt ccttgcatca tcccatttgc ttaatacact
     1201 gtaatagttg atattttgaa ataacatggc ctaaactttg aaataacatg actttaattt
     1261 tgaaataaca cataccaaat tttgaaatat ccgggttttc gaggctttct ttcactaaaa
     1321 ttatgcttag ttttaagttt tgagtcctta gttcttcata gttttgagtt cttagttctt
     1381 agttcttagt tagattcaac tcaaaactca taatccggga cgacccggac tagcgctatg
     1441 cgccaactca tatctcaaaa ctcaaaactc ataactcata actcataact aataactcaa
     1501 aactcaaaac tcaaaacctc ccccacaatc cgctccgctg tctttccgtc ccacaattcc
     1561 gggatgccgc ctttcttcca cttgccggag aacaagatat ccatggcagg cggaatggcc
     1621 gccgggtcag tgccgaggag ctcattggtg ccgatggtgc aggtctcggg gcgctcggtg
     1681 ttgtcgcgga gggtcatgca tgggatgccc atgacggtgg tctcctcggt gatgccgcca
     1741 ctgtcggtga tgacggcttt ggcatgtttg acgaggtagt taaattccag ataacccaga
     1801 ggttcgacta aatggagatt gggtgcagat aaggaggcgt gttccaggac gccggctgta
     1861 cgcgggtgca cagggaaaac cagaggaatg ccccgggatg aatctataat agtccggatc
     1921 agacgaacca gtcctgcctc ctgatccaca ttggccggac gatgcagggt catgacgatg
     1981 tagttgcctg cctccagatg gaatgtgtca tagagggacg gtcgacggag acggtccata
     2041 tttctgtaaa gggtgtcaat catggtattg cccacgaaga aaatctgatc ttctgtcttg
     2101 ccttcctttc tgaggttctc attggctgtc tcagaggtgg taaagaaata atcggttatc
     2161 gagtcggtca cgatgcggtt gatctcttcg ggcatggtcc agtcgcctga acggatgccg
     2221 ccttcgatgt gggcgacccg gatattattc atcttcttcg cggtgatggc acatgccatg
     2281 gtggaggtca cgtcaccgac gaccatgacc agatccggtc tgtcgtccat caatgctttt
     2341 tcatagccct gcatgatggc agctgtctgc tgagcctggg taccgccgcc tgctccgagg
     2401 ttgatgtggg gctgtggtat acccaactgg gtaaaaaagt cgccactcat cttttggtcg
     2461 taatgttgac cggtatggac caaccgataa ctgatatttt tcccttcggc ttttgctttc
     2521 tcaatcgctt cgatgacggg ggcgatcttc ataaaattag gccgtgcacc ggcaataatg
     2581 tctattttca aaatgtgaat tttaaaagtt ataaatgtgg ggcagaagta gggttaatta
     2641 tacaatattg gaattgaatt ccataattgt aaaatttgct taaaatctca tctcgcgcaa
     2701 tggaacgacg ctaaagcctg agcttctatt ttgcctgtga attccatcat agataaccgt
     2761 ccctccagat gtgtttgtaa ttttattcca gaataccaag cctttgaaat atccctggat
     2821 aatggtttgt ccggatttga tttccacggg ttgaatttgt ccactttgtt taattaataa
     2881 gtcaatttca ctcccgttac tatccctcca aaaatataaa ttgtgtgtct ctccccgatt
     2941 taaaaattgt ttctttactt ccagaattac aaggttttca aaaaacgaac ctctgaaagg
     3001 atgtaaggcg atctggtcag gatcattgat tccaaccaag gcccaggcta gccctatgtc
     3061 ataaaaataa agtttaggca tctttacaat ccgcttatta taattttcat aataaggctg
     3121 caacctgaat aaaacgaaac ccgcctccaa tacgctcaac caggaatcca ctgtttttac
     3181 atcaacaccc acctccacag ccaaactact catattcaat aattggccta tcctgccggc
     3241 acaaagtcgc aatagcttct caaatttatt taaatctcca atattgagaa tttgcctgac
     3301 gtcccgttca acataggttt ggatgtagtt tgcataccat tttgtagcag gaatgagttg
     3361 gtcatagatc gcaggataac cgccattgta catcagttgg ttagtactct gtggaatatc
     3421 ctttatttca tcgatagata aaggcaatag ttgaagataa gccacacgcc ctgccaaact
     3481 ctgggagata ttttcctgaa ggagaaaatt gtttgatcct gtcagtatat attttcctgc
     3541 ttctttctga ttatccagaa tctcctgaag gtaagaaaac atatcaggag atcgatgtac
     3601 ttcatcaaaa attgcaccat cctgataatt tgccaaaaaa cctctgggat cttcagatgc
     3661 aaataagcga acgtcaggat tttcaaaatt gacatatggt ttatccggaa aggcatcttt
     3721 tactaatgtt gtttttccgg attgtcgtgg acctataaca actacagctt taaactgatt
     3781 agccaatgtc aataattcct gttttgcttt tctttgaatc atgatgcaaa tatacaaatt
     3841 tggaattgta ttccataatt gtatgaaatt attctttaca aatagtggtt cttagttttt
     3901 agttcttagt tcttagttct tagttcttag ttaaattcaa ctcaaaactc aaaactcaaa
     3961 actatgtttt ctttgtagtt ttcacgatac ttgtgagcat attgataagc tcctgagatt
     4021 cttgtattag taatttaaat gatatttccc ttaattggct ttgctccaat aattcgagcc
     4081 agtactttgt ctcacgtgcc tcttttgatg ctatagacat tttgtgctta aaatcagccc
     4141 tgctttctcc tgctaaggcc tcgtttatat tggctcctat gctcgttcca ctccttaaca
     4201 actgtttaga aagtacaaat tcattctgct cctgaagtcg cttataaatc tcaattattc
     4261 caagtgcaaa cttgaaactc ctatctttaa caatactctc tttcatgtta ctaaaaatag
     4321 attcttaact aaaaactcaa aacttaaaac ttaaaatcgc ggacggccgc gactatagcc
     4381 atggacggcc aggactagcg ctatgcgcca gctcataact caaaactcac aacttaaaac
     4441 tcataactca caacttaaaa ctcataactc aaaacttaaa actcataact cataactcac
     4501 ataagttcac ccctacgaag cttgcatcct gctttatcgg aattttctac gctaatcgag
     4561 agttggattc atgttttaag ctacaattcc aaagcaataa attctttaat gcgaatcctt
     4621 acctatcctt gtccaataaa atccttcatt ggtaccatac catccggatg gcaacgacct
     4681 ttaacccttt acaaccaatt ctgccccctt ctcagcaatc atcaccgttg gagcatgtat
     4741 attactgcct gtaatggtcg gcataatgga ggaatcaatc acacgaagat tccggatacc
     4801 atagactctt aaccggggat cgacgacggc catattgtca attcccatct tacacgaccc
     4861 ggctgtatga tagaccgatt ctactttttt aaggataaaa cgacggatat cctcatcggt
     4921 ccggcaagaa gcgcccggca cttcttcttt cttgttgaag ggctgcatgg caggctgcga
     4981 aagaatattt ctggctatct tgacaccttt caccaaggct tccagctcca tttcatcgtc
     5041 gaagtaattg gcttctatac gcggatgcgc aaaaggatca gccgaagtca gccgaatccg
     5101 ccccctactg ttgggtcgca tatgacagat gtgcaaaaca tatccggcca gcgtgggtgt
     5161 gagtcccgct ccgtgaggct gcatgcgtaa agaggcaaac tgtaactgga ggtcaggtac
     5221 ctctaacgat ggatccgatt taataaagcc acctgcatcg gtaggcgtga agatgaagtt
     5281 gctgttcttc ttgtaaaacc tggacatgaa acgaatcata tacggccagg ggaaggtggt
     5341 caacgatgtt cccgacttat cccgatagcg tagtattaca tccggatgat cctgtaggtt
     5401 ttcgcccacc ccgggaagat ctttgactat ttcaataccc aactgctgca gttgagcggc
     5461 cggaccaatc cccgagagca tcaatatctg cggcgaacca atgatgcctg cactcagaat
     5521 aatttcccgt ctggcattta aagtgcacag gcgacgggca tttttcaggt cgaagtattc
     5581 cacggcgtat gctgtaccat cattcataag caccttattg acatgagctc cggtgatgat
     5641 ggtcaggttt ttgcggtggc gaataggatc caggaaggag acagccgaat gagcacgttg
     5701 tccatccggt gtttggttga tctgaaagaa atccacccct tcgtattgct ctccgttgat
     5761 atcccgattg tggggtatgc ctgcttgttt gcaagcttcg ataaaggccg tactagccgg
     5821 aaagtgaaag ttaggatcga tgacatccat ctcacctgtg tcattgtgat aggcatcccc
     5881 tccgcggtgt tgccgctcag atttcagaaa atacgggagc acagacctgt agtcccagcc
     5941 ttcattgccc agagccgccc accggtcaaa atcagatggg tgtccacgtg tatagatcat
     6001 ggcgttgacc gcactggaac cgcccaatgt cttaccgcga ggccagaaaa agcgccgttt
     6061 tcctgttttg tgattgcctt ccgcataata tttccaatta tatttagggc tattgagtag
     6121 gtaaagcatg ttgaccggat tggtattgtg gatgaagtgt gagtcatccg atggccctgc
     6181 ttcaagcaga cagacctctg tctgggcatt gtccgagagt cggttggcga ggaggcatcc
     6241 ggcagatcct ccgccgatga tgatgtaatc aaacgatttc attgacgcat atcaatttca
     6301 aatgtgtata tagcatgatg gtgagtctca tacggaatgg taatcaggta gaatgctgtc
     6361 cacacacaga ttcaggtctt tttgaggcgg cctttcggga caatctatca tcaaggtcgc
     6421 gcctacagcg cttatatccg aaaacattga attatacaaa aaaacattaa atccttagga
     6481 tatcaaaatc aatcagatca aaagcctcgt tagaggcgac cttatggtag catgtcagca
     6541 atcgcgaagc atcaagccct gtaggggcga ccttataaaa aacaaggcgt gcattaaatt
     6601 ataccataaa gccatgaagg caggttaaga catgcaagac ataataatcc ttatgctcat
     6661 aatgattcaa aaaaaataaa attcaatatt aaatctttaa cacattatgc atatcgacta
     6721 atcatgtcaa tccaaaaggt cgcacctacg gagcttgtat cctgctttat cttaatatgc
     6781 taccataagg tcgcgcctac agcgcttaaa ttcgaaatca ttgaattata caaaaaaaat
     6841 taaatcttcc ggatatcaaa atcaaccaga ttaaaaagcc tcgttagagg cgacctgatt
     6901 atcaagcaaa gacataaata aaattactat tgtgcagatt taagatttca atctaccctt
     6961 taatggttat aactctgatc acagatattg accaaaagtc ttcataaatc actcttcctt
     7021 taaaaacaat gtcatttccg ttactaatgg aagatattat ccatttctaa tggaagatat
     7081 ctttcactgc aaatggaaga tatggcaaat cagacgatgg taatgtaatt gccaaaaacc
     7141 tatatactgc acgaaaaaac ctccacgctc tgttgcaaat tactcattca agtaacctga
     7201 ttaaatattt ccagcattct cagtgccagt tttcttctgt caaagtccag ggccaggcgc
     7261 ttacaacctt cctgaagttt tccatataac tgtgcatcgg attttaatct atacactgca
     7321 tccaaaaaag ctgcctcatt ctccggttca aagcagatac cggcatcgta ctgttcgatt
     7381 atgctttttg actctccttc gacgcccagt aaaataggct tctgcatgga tgcattctca
     7441 aaaatcttgg aaggaatcac cgtctgaaat gtttccgatt tcatcaggtt gactagggcg
     7501 acatctgtaa tgctgatata atccgcaatt tcatttttag ggacagagtc aaggaaggtg
     7561 caattcttca cccttaactg tactgccaga tttctgacat tttccttctg tgcaccattc
     7621 ccaataaata gaaaatggat gtcttcatct gttatcttag ccgcacactc aacgataaag
     7681 tcgagcttat gcgccatacc atgtgtcccg agatagccaa atataaactt tccttccagg
     7741 cccaattgag agagcagttg cctgtttttt tcaaccggtt taaacttctc catcagaacg
     7801 ccattcttga cgactgaaat cttgttttta tcgattccgc ggcttgctat tttatccctg
     7861 aaagcatcag tcacgacaat aattttctta gcgcttttgt atagcctttt ttccacccat
     7921 tcgagatagg aaaaaatcat attatcactc ttcatggcgc tgacagcctt gatggattcc
     7981 ggccagaggt cacgcacctc catcacccag ggtttccgct tacataaaga caaccatcgt
     8041 ccgcttactg ccgtaaaaaa ctggggcgag gtagctacta tcaggtcagt ttttacgaat
     8101 aaaccatgca aaaaagaggt cactccgaaa ctaacgtaat ccagaatccg ttttccaaac
     8161 ccttcattgg cactgatata actccataca cgaatcaccc ggataccatc catcgtctct
     8221 ttctgccgcc atttgttttt ataattgtcg aaaaccttac cctgtgggaa attgggtacg
     8281 catgtaatca ccgtgacctc cacaccacga cgaacccatt cgatgcaatg ttcgtacgtc
     8341 cggctggcag gagcgttgac ttcaggggga aaattgtctg ttaaaaataa tattctcaaa
     8401 gcgatatgct tactttcagt tttttatcaa attttacttc cagacagtct gatacaatat
     8461 gtttattaaa ttccggagca tagttgaaat tcattacttt tttagaagtt tcaccctctg
     8521 tgataattga aatgtcagaa gtatatccag gcttcacacc ggggtaaaaa tgaaaaaaac
     8581 atctattatt tatcggagcg tgactacctt taacttcatc ctcaataatc aaggaattgc
     8641 tttccaatgt aaatcttctc gtatgcgaaa tgttcttatc ggaatatccc cgaactactg
     8701 cttcaatatg atttttttca tcacaaagaa cttcaacctg aaccctcctt gccaccctga
     8761 atccacccca tacttcagac tgattacaat cattgatgac cacggtgttg tgcgctgatg
     8821 tcgatctttc ctgctgcctg acctcattta cctcataggt agatgttcct gtatcgacga
     8881 taaacgggcg tccatccact tgtaattcaa aactcaaaga atctgcatgc gcatgacccg
     8941 gaatataatc gggaccaata ttcccaacgt ctataataca atccaaccga tcgtttttca
     9001 atctcctgta tccggattcc tttaactccg tttttctcac atgtaaacct aattccttgg
     9061 cataggtaaa taactgaata cttgtcggtg cgatgttatt cgcactgtca ttgaatagag
     9121 gaatagtgcc atcatcgaag gtcatcgatg ccaaccatcc aagcattttt tcagccttat
     9181 cggtaagtaa cggtaataat tgccgactat aatgcccgtt tttatgaatg agttgaatac
     9241 aatccagcac tctgaaatgc atgtgctggt gatacatggg gctcaactca aaatgcccgc
     9301 catccggcaa gatctgttct tcaagctctg acctcaggat cctttctgct aaacccaaca
     9361 atttatcatc attcaggtaa cttccggcga aaaacagtcc gaatccattc tccagtaagt
     9421 gatttcccag caaatgatat tccgggtgat gataaagccg ccacgcatcc tggtggatta
     9481 cctgatcaat ccggctatct tgaatgtcat tttcagaaat aaattttatc cagttgagca
     9541 agcgtaaaga tataggaaat gactccctgc catcctttat ttcatcataa tgattgataa
     9601 attcatgaat cgattttagt ccgtcttcta cagaaatatc ctcctgcatc agccattcaa
     9661 aataattcaa attataagtc cacaacttgc cgaaagcctc atagttccag tctattttat
     9721 ccccaaagtc tttctcgaga tttataaatc tgaattttct tcctcccatc catgtttgtg
     9781 gaaggatgat cctatttgaa aaaatcagct tttccgcaac gtctttaaaa acaggtattt
     9841 tccgattaat ccttagcttt ttttcaagca agtaataaat ccggtattta atctgaatcc
     9901 acttcagata acggactgtg tgaaaataaa gaagcagttt gtgcaagagg agaattttaa
     9961 atcgtcttta gttctgattg aattatacaa aaccacttta tcacaattcc atccatcttc
    10021 cttctgtcag ggactctacc gccagaatag cagctttgga agtattgtat aaatcatcaa
    10081 attcgatgag tggattacca ccattcttga cagactgtaa ccatcggctg aactgctccc
    10141 tgtgcccctt atcctgtgtt tttttcatat cgcttctttt gtatccataa aatgaagctt
    10201 ttctgaagtt gtcaatcagg atattctttc cttgtgaaaa tatttcgatt ctctctttcg
    10261 ggtatgcttt attacctgtc gcaaagtaat taatcactcc cgtagaacca tcttcatatt
    10321 ttagtaggat ggatgcattg tccgtactta cggaaggatg agttccgatg gcattcatga
    10381 tcactttctc cacccttgaa cctgtaaaat aggttatcag atctatgtag tgacaggctt
    10441 cgcctatgat tcttccccct ccggattcca tatcctgaac ccaggaattg gcgggaataa
    10501 atccggcatt catggtagcg ataacctgta tcggtccatc gcccagacac tccctggctg
    10561 caatggaaaa tggtgagaac cgtctgttaa agccgactgt aagtgatttt ccacacgaag
    10621 tataagcatc ctttacagca tccagctctt ccatgtttaa ggcaagtggt ttttccacaa
    10681 agacatcttt tccatgttgc aaagcttcga tgacctgtcc tgcatgagca ctatggcgtg
    10741 tagtaatcag cactgctctt acatccttat cttccaggat atcctgatat cgggatgtag
    10801 agtgagctat gccatacttt ttagccagag tggtaccgga tagccctttt gcactggcga
    10861 tatacttcat atccgccttt aattctttca gtacaggcac gatcatcgca cccgtaaagt
    10921 tacctgcgcc gatgatgcct atctgtgcac ttccaccgga gaagtcatgt gagacgacag
    10981 atactgacgt tttagaaaca tccacatcac cgggatattg tattatagat gcgatagagc
    11041 cacttttact catatcacca tagatggcat tatattcatc cagaattact cgttctgtaa
    11101 taagctcatt caccttcaat cctcctccga ctatagcact gagtactgct tcaaagttgc
    11161 gctgttccgt ccaacgtaca aagccgatgg gataatccag gcctttctgt tcgtaatgct
    11221 catcataacg tccgggtcca taagagcagg atacctgaaa gctcaattcc ttttcataaa
    11281 agtcagaccg cttaatatcc aggccaatca ctccgaccaa tacaatcctt cctcttttac
    11341 gacacatctg tgcagcctga ctgatgacct catcagattt ggtagaagca gtaatcaata
    11401 caccgtccgc ccctattcca ttggtatgac tcatgatggc agcaggtaca tccatttcgg
    11461 aagaaacttt aatagcttct attccgaatg actttgccag atcaactttt cgaccatcaa
    11521 aatcaagtcc tataaccttg catccattgg ctttgagcaa ttgtgctgca agtaagccaa
    11581 tgaggcctag accagttacg acaatggtct cgccaaatgt tgggttgatc agtcgaatgc
    11641 cttgaagagc tatggctccg ataactgtaa aggaagcctc ttcataggag acttcatccg
    11701 gaattttagc agtgagattc tccggaacac agactatttc agcgtggttt ccgtttgaaa
    11761 taaccctatc gcctatggta tatccaagca ctccttttcc gatggcaatc acctctcctg
    11821 cattgcaata tcccagagga agtggttcgc ctaatttcct aaaaactgca tccatggtag
    11881 gtctcagccc atcggtctta atcttctgaa tcacctgcat taccttttcg ggttgctgac
    11941 gcgccttact gatccagcca gccttcccga attctacgag catacgctcc gtgccaagtg
    12001 aaacaaggct gcaatgggtc tttatcaaca gacatcccgg tcttacctgt ggtacgggca
    12061 cttcttcgag gatggtttgg cctgatttga gatcctggac tatttgtttc attaaatcga
    12121 atttaaattt tggggaatcg ttacaaagtt cttcatctgc attatcctga cacttataac
    12181 ttatccctta acgctcttaa ctctttacta ataaattctt gtagtttttc ggtactgggc
    12241 aattccagta tatacttgct tacaaacaga tctttgtctg tatcagcaac tgcatattcg
    12301 actaattctt tattgttttc agttactaaa agtaatgcca caggcggatt atcgtcctta
    12361 agcatgaagt tctttttata atagttgatg taggttttta attgtccgat gttttcatgt
    12421 tctacttctt tcatcttcaa gtcaatcagc acatgacatt ttagtattcg gtgatagaac
    12481 accaaatcaa tgtgatagta ccggtctcca atgatgattc gtttttgttt tgcctcaaag
    12541 caaaagccgt atccaagttc taataaaaat tcttcgaggt ggtcaataag ggcttgttcc
    12601 aagtcagatt catataccac atctttggct tttagaccta aaaattcaaa cgtaaacggc
    12661 gatttaataa tatcagtagg cactaaggca tgcgctttat cattcaccaa acgactaagt
    12721 ttttcaggat tggtactcat accactccgc tcaaaataca aggaatttat ttgtcgtttc
    12781 agttcgctca cactccaggt ccctttcata cattctattt cgtagaaagt gcgttttaac
    12841 gggtcttcga tgggaaggag taagacaaaa tgtgaaaaag ataaagaatt tatcaattgg
    12901 ttaggcggaa cttgcaggcc ttccggcaag gtactaatga attcgtcaga ggctgtctga
    12961 cgaattttta cagtctgatt atcatgtatt tgtaattcgt cagtcgacaa atgacgaatt
    13021 gagctaaatc caatttgctg caattgttca gacactatct gagcaatttg aggatagact
    13081 aaataaaatt gacggctaag tttgaggttt gtttctgcca atccttttac attcaaagtt
    13141 ttggctaatt ccgcaaataa cctttcacca tatgttgctc tatcagaacc attttgctca
    13201 aattccacga tataaaaacc gatcaaccag ttgcgaatgg tcaaatgtat attcactgac
    13261 cttatcgcac tctgttgaag ttgattatgt gttgcttgta ttgtatttac taacaattca
    13321 aagttcatag tacaaggcta ttcaagatta tatacattct aggaatcaat taaatgctta
    13381 tagtttagac ctataataga ttaaaatcat caatactcaa tgttgaattg acatacaata
    13441 tcattttttc tggttgctga cgcgccttac tgatccagcc tgccttcccg aactcaacga
    13501 gcatacgttc tgtaccgagc gagacaagaa tacaatgggt ctttatcaac agacatcccg
    13561 gccttacctg tggtacgggc acttcttcga ggatggtctg gcctgatttg aagtcctgga
    13621 ctatttgttt cattgattgt ttaaaatatt tcgtttttca attcggttgg ggtaatttat
    13681 aagtacagcc cgatatttac ccttaaatac gaataagcta cctgcagcgg caccgattat
    13741 cttataatcc ttaattaagg atttaatgtc ttcatgagag ccggctcccc ctaaaatcgt
    13801 tataggaata tctactgcat ccctcactaa tttaactaat gtattatcat acccctccat
    13861 ggtaccatct ctgtcaatgg aatttattac gatttcaccg acaccgatat cattcagttc
    13921 tttcaggaaa ttttctaatt tttttcctga cttctcccgc ccatttttag tatatatttc
    13981 ataagtggtt ttgccaagga gtcctttctt cttcttaaca tcaatcacta caacaacact
    14041 ctggcttcca acagcttcgc ccatttttct caataatgag gtatcattaa tagcggcgga
    14101 acttaaagcc actttttcag ctccgagttt gataatgttt tttgcctggt ccacagttgt
    14161 aactccacct ccataacaaa aaggcattct gcattcaata gcaagattct ttatcatcgt
    14221 gaaatccgga cccctccctt ctacggtggc atcaatatcc aataccatca attcatccac
    14281 ctctttttca ttgaatattt taactgcgtt aattggatca cctacatatt tagggtcttt
    14341 aaactgcact gttttgacca gacctttttt atgtactaac aggcatggcg ttattctcgg
    14401 tctaagcatt cagatttgca aaatttttaa ggagggtcac tccattttga tgacttttct
    14461 cggggtgaaa ctgtacacca tatatatttt catggtttac ggatgaagca aattcttttc
    14521 cataaaaggt tgttgacatg atatctcctg gattgtcaca ttcaaaataa taactgtgta
    14581 aaaaatagaa acccatttct tcatctattg aatgaaagag ttcagattgt ttcgaaacct
    14641 tgatagaatt ccagccaagg tgaggaatct ttggtttcaa ctccaataaa ttaacatcaa
    14701 tctttttcac tattcctttg acccatccca agccgggaag tttgccttcc tcacttcctt
    14761 cggctaaaat ttgcattcca acacatacac cgataaccgg
//
LOCUS       DAOQMC010000029        14443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1526_length_14443_cov_4.528287, whole genome shotgun sequence.
ACCESSION   DAOQMC010000029 DAOQMC010000000
VERSION     DAOQMC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14443
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_14443_cov_4.528287"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKV31_02170"
     CDS             complement(<1..463)
                     /locus_tag="PKV31_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNB91688.1"
                     /translation="MATRKPKVTPLSFTQKVRQLISDDRIPKISGLFCYLIAIFLFIS
                     FTSYLFTWEADNSFLRPFKWDNLFDPDIQAQNWFGKFGALISNAIIFWTFGISSFVII
                     FLLIRIGYRLINGKGSARIWPMFRNVFFTMILGCAFFELILPEASFPYSGAY"
     gene            complement(617..2200)
                     /locus_tag="PKV31_02175"
     CDS             complement(617..2200)
                     /locus_tag="PKV31_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNB91689.1"
                     /translation="MSRLQEINRRRTFGIISHPDAGKTTLTEKLLLFGGAIQVAGAVK
                     SNKIKKSATSDFMEIERQRGISVATSVMGFEYKDLQINILDTPGHKDFAEDTYRTLTA
                     VDSVIVVIDVAKGVEPQTERLVEVSRMRNTPIIVFINKLDREGLDSFDLLDEVEQKLG
                     LTACPLSWPIGMGQHFQGVYNIFGQQLSLFKAHGKQTEDDTLIIKGLKDSTIDEAIGQ
                     RAAEELREEMETVAGVYPEFDRDAYLNGKLSPVFFGSAVNNFGVKELLDCFVQIAPPP
                     LPRETEERIVKPEENSFSGFIFKIHANMDPKHRDRIAFLRICSGEFKRNTNYYHVRKE
                     KQMKFSNPTTFMASKKELVEEAFPGDVVGLYDSGNFKIGDALTEGEILHFKGLPSFSP
                     EQFRFVNNADPLKSKQLAKGLEQLMDEGVAQLFIKEDNGRKIVGTVGALQFDVIQFRL
                     EHEYGAKCTYEPVNLYKACWVTADKQNDMDAFISRRKKDIARDKDGALVYLAESAWSL
                     KTVRENHPEIDFHLTTESV"
     gene            complement(2295..3020)
                     /locus_tag="PKV31_02180"
     CDS             complement(2295..3020)
                     /locus_tag="PKV31_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNB91690.1"
                     /translation="MGVPDWLERTELLIGEENIHKLKNARILLVGLGGVGSYAGEFLV
                     RAGIGSLIIIDGDRVDPTNINRQMQALHSTIGMSKAELLRQRFLDINPGLQIEIHTSF
                     MEPADMELLLQQVEIDFVLDCIDSVLPKLTLIKAAVRAKVKVISAMGAGGKFDPSKVK
                     VADISKTRECKFAQTVRKRLKYLGIRKGILTVYSEEIQPKSALQYTDGSAYKRSFYGT
                     ISYMPALFGLRMAAEAISRIIKA"
     gene            complement(2981..3679)
                     /locus_tag="PKV31_02185"
     CDS             complement(2981..3679)
                     /locus_tag="PKV31_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNB91691.1"
                     /translation="MNPHAPFIDFHNHNIWHSDDVIEVVSVHDSQVKKRIYYTVGYHP
                     WWTEDLLSEKNLDMMRSSYISDPYCLGLGEFGLDSLKGPGLDIQEENFRKQIRIANEV
                     SSPVVIHCVRAYDRIIRLRKELGKTDWVIHGFVRNKILARQLIDAGFYLSVAPCERMK
                     PVFEEMLAYVPMDRIFIETDSEFTLNIQQRYTIFAQIREVDVAALKMQLFDNFKTFYT
                     FKWAYLTGLKEQNC"
     gene            complement(3710..4414)
                     /locus_tag="PKV31_02190"
     CDS             complement(3710..4414)
                     /locus_tag="PKV31_02190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNB91692.1"
                     /translation="MQLFWGKRINDNVILVEGDEVLHLTKSLRKKVGDAVDLIDGSGN
                     IFHSVITEIQRGKLVAVNMSETPKVNHWKKRLHIAIAPTKNADRMEWFVEKAVEVGID
                     TISFIQCERSVRTNLRMDRIARIVQSAMKQSYNIINPTIQEKSFDQLLTGFNPTAMVG
                     LIAHCEDGIKQAFSDIQFDKPDTWILIGPEGDFSGQELENALRIGFVPLSLGRSRLRT
                     ETAGIYAVSIFRNINI"
     gene            4541..5878
                     /gene="rseP"
                     /locus_tag="PKV31_02195"
     CDS             4541..5878
                     /gene="rseP"
                     /locus_tag="PKV31_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNB91693.1"
                     /translation="MDVIVMIAQLILSLSILVILHELGHFIPAKLFKTRVEKFYLFFD
                     PWFSLFKFKKGDTEYGVGWLPLGGYVKISGMVDESMDKEFMKRPPEPWEFRSKKAWQR
                     LIIMIGGVTVNFILGFLIFGLILYFYGEQKLPVSEMQYGIATTQLAKDIGLKDGDKIL
                     QIGNNDFKYFNSGELAKDILLDNAKTIRVNRNNLIMTVHIPDSAVQKIQSNQKEAIGM
                     IAPRMPIIVDEVMQGSGGEKAGLQPNDRVISVNNVSTHFFHEMTNELQANKGKKVNIG
                     VIRDGNTVMLEQVQLSDQGLVGFSPQPISHFYKLDTIKYSLGKALPAGFRNGYDFLAT
                     QISAFGQMFSGKIKASESLGGFASITKLFPTEWDSQKFWNITAILSLILGFMNLLPIP
                     ALDGGYIMFLLWEVITGRKVNDKVMEIATTAGMILLIGLLLYANGLDVIRALK"
     gene            5878..6390
                     /gene="hscB"
                     /locus_tag="PKV31_02200"
     CDS             5878..6390
                     /gene="hscB"
                     /locus_tag="PKV31_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNB91694.1"
                     /translation="MDYFEWFGLDRKFNIDQAGLRKAFLTKSMELHPDKTGLNENKAM
                     ELSEYNNKAYGILKDSYKTLDYILHLEGIVLADEKYQLPQDFLMEMLELNMEIEDAGE
                     SGDKEKLNKIKSDLETKGIEEDANMEILKNQYDSRSIDPQGYESLKKFYFIRKYLLRI
                     EESINTFASH"
     gene            6422..6505
                     /locus_tag="PKV31_02205"
     tRNA            6422..6505
                     /locus_tag="PKV31_02205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6456..6458,aa:Leu,seq:caa)
     gene            complement(6868..8226)
                     /locus_tag="PKV31_02210"
     CDS             complement(6868..8226)
                     /locus_tag="PKV31_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB91695.1"
                     /translation="MYFKNILETIGNTPLVRINKITSDLPCTVLAKVETFNPGHSIKD
                     RMALKMVEVAESEGKLKPGGTIIECTSGNTGMGLALVAAVKGYKAIFTTSDKQSKEKM
                     DMLKAMGAEVIVCPTNVEAADPRSYYSVARRLSQEIPNSYWFNQYDNPANSQAHYETT
                     GPEIWEQTEGKITKLVVGVGTGGTVSGTAKFLKEKNPDIEIIGIDTYGSVFKKYKETG
                     IFDENEIYPYITEGIGEDILPENVDFNLIDYFEKVTDKDGALMARRLAREEGLLLGYS
                     AGSAMAGLLQVKNRFRPDDVVVIIFHDHGSRYVAKIFNDDWMRDRGFISMDLRLSDLV
                     KRKPKSGLITAQKGSKLREVFQMMKENDISQVTVNDQGEIVGSLTESDIISSLLSNPD
                     NIDNVSVGEIMNEPFPVLEEALSVKELSRYISKKIPAVLVRDGAGVNHILTQYDIIQH
                     LG"
     gene            complement(8436..9452)
                     /locus_tag="PKV31_02215"
     CDS             complement(8436..9452)
                     /locus_tag="PKV31_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB91696.1"
                     /translation="MPSRKLLYVVSWVFVISLFSQKAGATGDSLSVLTKNDTIFIQRD
                     SLGVIYFYHRVMPKQTMYSLCRFYGIKQSQLMTLNPVLKVRGIQIDELIRVPISARLI
                     RVESEPKKRENFAPVVYEVSPGEGLYSVSRNYFGIKPEKLMQINGMSAPDLKLGQILL
                     LGWIPLDVFNKINENVVADKGRPKSGTKADNAKTDTKKPGPNALGTTSTAKEREIAKE
                     AVQDAVEKTTSEEAKSTPASNREKFESYEGTKAFEQGLAFWHKELKGSNGLFVLHRTA
                     PRGSIIKITNPMFNSTVYAKVVGTVSPNSYSDDVMMVVSPEVAIKLGARDARFFAKIA
                     YIKG"
     gene            complement(9932..12730)
                     /locus_tag="PKV31_02220"
     CDS             complement(9932..12730)
                     /locus_tag="PKV31_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNB91697.1"
                     /translation="MKQLLVSVGLILSIGLHAQVTIDPPFPNINSSITVYFDATKGNQ
                     GLKDCNCTVYAHAGLITDKSSSGSDWKYVIGNWGTDDSRVKMNKVSDNLYSLTYNIKS
                     FYGIPESTIIRKLAFVFRNLNGSKEGKTESNGDIYYDFPDPNAGLQYLIQSPSLESRL
                     VSKNSVLNLSVLTSIPADLRFTINGQDVVTTHDVSMSYQYIADQVGTFRFEIGIHAGN
                     EGLDTSFVWTVGGDPEISEVPDGLLLGCNKVEEGKAIVLLEAPRRNIAFLVGNFSNWA
                     PLPEYQMKQSPDGQFLWGEIDTVGLQGDLDYQIWFGDGLKIPDPLSVEILDDVNDKYI
                     PAAVYPDMPSYPEGKTTGLVSHLKLNADYDWQDEAFVKPAVGDLVIYELLVRDFLASH
                     DFDDLIDTLLYLKRLGVNAIELMPVSEFEGNISWGYNVSLHNAIDKYYGGASKLKKFV
                     DAAHGLGFAVIADVVFNHAFGQNPMVRMYWNAAQNKPAIDNPWFNPDAKHPFNVGYDF
                     NHQSAATRRYVSQILREWIREYHFDGYRFDLSKGFTQVNNPNDVGAWGQYDPSRIEIL
                     KYYSDQIREVDPQAYVILEHFADNQEEKELSDYGMMLWGNINYEYAESVKGNSASLNR
                     SYFRQRGWRKPHLVSYMESHDEERVMYKALKEGRQSAGYNVRDLKTALGRVGMAAAFF
                     YTIPGPKMLWQFGETGYDYSINHCPDGTVNNNCRVDPKPIRWDYYHQQNRLELFAVTA
                     GLTHLKHSFPDLFDDVEIDYDLNGLFKYIVMDQNDLKMVVIGNFDVTDGSKAITFPTD
                     EWYYNYMGRDSIKGNTSLRQITLKQGEYKVYLNKKVENPGITLSDKGTLIQDVEFTVY
                     PNPAESELHIQLPERIFYETVSVTVSDISGRIVYHDNRLSGQTTITIPLNSVKPGVYL
                     VRVSNPSVFGVKKVMIVR"
     gene            13258..13947
                     /locus_tag="PKV31_02225"
     CDS             13258..13947
                     /locus_tag="PKV31_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB91698.1"
                     /translation="MQFFQLFLLQIPDTTAIQSIALPTQKLTFFELLLKGGVIMIPLV
                     ILSIIALGLIIERWLSINNLTKVEANFFNNIKSMLSNGNVKGAQDFAESNNTSIGKVI
                     GEGIRYIGYPYSEIETYMSDTANIELGKMENRLNYLGIIAGVAPMLGFVGTIAGIIKI
                     FYNISITDNISIGVISGGLYEKMIASGTGLIVGIFAFIGNQMLQAKIDRYALKMQDMV
                     KSFRSVLLQKG"
     gene            14009..14404
                     /locus_tag="PKV31_02230"
     CDS             14009..14404
                     /locus_tag="PKV31_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB91699.1"
                     /translation="MKINRKRHKPHVFVDSLSDIMFFLLLFFLIVSTVANPNVVKILL
                     PKSGNTEVLSKKQITLTITADKRYFIDKQEYTIDQLEEAISQVTANVTEPTIMLRLDK
                     SLTVQDLIDVMSIGAKLKIKMVLATDKKS"
BASE COUNT         4403 a         3104 c         2754 g         4182 t
ORIGIN      
        1 cataagctcc actataagga aaagatgcct ccggaaggat caattcaaaa aaagcacagc
       61 caagtatcat ggtgaagaaa acgttgcgga acatcggcca aatccgggca cttcccttgc
      121 cattgatcag tctgtatcct atgcgaatta acaggaagat gatgacgaag gaacttatgc
      181 caaatgtcca gaaaatgata gcgtttgata tgagggcacc aaacttccca aaccaattct
      241 gagcctggat atcaggatca aaaagattgt cccatttgaa aggtctcaga aaactgttat
      301 cggcctccca tgtgaataaa taggaggtga aagaaataaa tagaaaaatt gcaatcagat
      361 aacaaaacaa gcctgatatc ttgggaatgc ggtcatctga gatcaattgt ctgaccttct
      421 gggtaaatga aagtggtgtt actttaggct ttctcgttgc catgagacct tgttgtgttt
      481 tgtagttatc ttatcagaat aatggcaaat ataatctatt cccctaatgt tcagggacaa
      541 aaatagtaaa atgttttata tatggagata tttttctcta cttcattatt tggttactaa
      601 aatccataaa tattaattaa acggattccg tagtcaaatg aaaatcaatt tcgggatgat
      661 tttccctgac ggtcttcaat gaccatgcac tttctgctaa ataaactaag gccccatcct
      721 tatcccgggc tatatccttc ttcctcctcg aaataaatgc gtccatatca ttttgcttat
      781 cagcagttac ccagcaagct ttgtacaaat taaccggttc ataagtacat tttgcgccat
      841 attcatgctc caacctgaat tgaatcacat caaactgcag ggcaccgact gttccaacaa
      901 ttttgcgacc gttatcttcc tttataaaca attgagccac accttcatcc atcaattgtt
      961 ccagcccttt ggctaattgc ttggacttca aaggatcggc attgttgaca aaccggaatt
     1021 gctcgggaga aaaactgggc aatcccttga aatgcaggat ttctccttca gttaaggcgt
     1081 caccgatctt gaaattacct gaatcgtaca aaccgaccac gtccccggga aaagcttctt
     1141 cgacaagttc ctttttggat gccataaaag tggtaggatt gctgaatttc atttgttttt
     1201 ccttacggac atgataataa ttggtattac gcttgaattc tcctgaacag attcggagga
     1261 aagctattct gtcacggtgc ttaggatcca tattggcatg gattttaaat ataaagccgg
     1321 aaaaagaatt ttcttcaggc ttgacaattc tttcttctgt ttcccgcggt aaaggtggcg
     1381 gggcaatttg cacaaagcaa tccaagagct ccttgacgcc aaaattatta acggcactac
     1441 caaaaaatac aggggatagt tttccattca aataagcatc ccggtcaaat tcaggataaa
     1501 ctccggctac agtctccatt tcctctctga gttcttcagc tgcacgttgt ccaatcgctt
     1561 catcaattgt gctatctttc aaccctttga tgataagtgt gtcgtcctcc gtctgcttgc
     1621 cgtgtgcttt aaacaaggag agctgctgac cgaaaatatt atatacaccc tgaaagtgct
     1681 gtcccattcc tatcggccat gaaagcggac aagcagtcag gccgagtttt tgttcaacct
     1741 catccagtaa atcgaaagaa tcaagacctt cacggtccag cttattgata aaaacaatga
     1801 ttggagtatt gcgcatcctg cttacctcaa ccagtctctc tgtctgaggt tccacaccct
     1861 tggcaacgtc aatcactacg atgacactat ccacggctgt cagggtacga taggtatctt
     1921 ctgcaaaatc cttgtggccg ggagtatcca aaatattgat ttgcaagtct ttgtattcaa
     1981 atcccattac ggatgttgcc acagagatac ctctttgtcg ctcaatctcc ataaagtcgg
     2041 aagttgcgga tttcttgatc ttgttggact tgactgctcc ggcaacctgg atggcaccac
     2101 caaaaagcaa caacttttca gtcaacgttg tttttcctgc atccggatgg cttataatgc
     2161 caaaggtcct tcttctattt atctcttgta acctgctcat aatctcttca atcagggcgc
     2221 aaaagtaatg aaaaccgcta aatagcagaa aggaaatcca taatccatta atgtattggc
     2281 aatgtaaaaa aatctcaggc cttaattatc ctcgaaatag cttctgctgc catcctcaga
     2341 ccgaatagtg ccggcatata agaaatcgtc ccgtagaagg atcttttgta ggcactgcca
     2401 tccgtatact gtaacgccga cttaggttga atctcttcag aatacacggt gagaatcccc
     2461 tttctaatac caaggtattt taagcgcttc cttacagtct gggcaaattt acattctctg
     2521 gtctttgaga tatcagcaac cttgactttg gagggatcga atttacctcc tgctcccatg
     2581 gcactgatta ccttgacttt agctctgaca gcagctttga tgagagtcaa tttaggtaat
     2641 acactgtcaa tgcaatcgag tacaaaatca atttcaacct gttgtaacaa caactccatg
     2701 tcggcaggct ccataaagga agtgtgtatt tctatttgca gacccgggtt tatatctaag
     2761 aaacgttgcc tgagcaactc tgccttggac atgccaatag tgctatgaag tgcctgcatt
     2821 tggcggttga tattggttgg atcgacccta tcgccatcta tgataatcag tgaaccaatt
     2881 ccggctctga caagaaattc accggcatag ctacctacac caccaaggcc aactaatagg
     2941 attcgcgcat ttttcaattt atgaatattc tcttcgccaa tcagcaattc tgttctttca
     3001 agccagtcag gtacgcccat ttaaaagtgt agaaggtttt aaaattatca aacaattgca
     3061 ttttcaatgc tgcaacatcc acctctcgga tttgggcaaa aatagtgtac cgttgctgaa
     3121 tattcaaggt aaattcacta tcggtttcaa taaaaattct atccatcgga acataagcaa
     3181 gcatttcttc aaacaccggc ttcattcttt cacagggggc aacggaaaga tagaaaccag
     3241 catcgattag ttgtcgggcc agaattttat ttctgacaaa accatggatt acccaatctg
     3301 ttttacccaa ctcttttctt aatcggataa tcctgtcata tgcacgtaca caatggatta
     3361 cgaccgggga tgaaacctca ttggctattc tgatttgttt gcgaaaattt tcctcctgga
     3421 tatccagacc aggacctttc agactgtcaa ggccaaattc acccagaccc agacaataag
     3481 gatcactgat gtatgaagac ctcatcatgt caaggttctt ttcagaaagc agatcctctg
     3541 tccaccatgg atgataacct actgtgtagt aaatccgctt tttaacctgt gaatcgtgta
     3601 cactgacaac ctcaatcaca tcatctgaat gccagatgtt atgattgtga aaatcgataa
     3661 aaggggcatg cggattcaat atgctattaa actgataaat ttcaatgtat tatatattaa
     3721 tatttctaaa aatagaaacc gcataaattc cagccgtttc tgttcttaga cgagaccggc
     3781 ctaatgacaa gggtacaaat ccaatacgca atgcattttc aagctcctga cctgaaaaat
     3841 ctccttccgg gccaatcaga atccatgtat ccggcttatc aaactgaata tcactgaagg
     3901 cttgttttat tccatcctca caatgtgcaa tcaggccgac catagcagtt ggattgaatc
     3961 ctgtcaataa ttggtcaaaa gacttctcct gaattgtcgg attgattata ttgtacgatt
     4021 gtttcatagc ggattgaaca atccgggcta tcctatccat ccgtaaattg gttcggacag
     4081 atctttcaca ttggatgaag cttatggtat ctattccaac ttcaacggct ttctcgacaa
     4141 accattccat tctgtcagca ttcttggtgg gcgctattgc tatatggagg cgttttttcc
     4201 aatgattaac cttaggagtc tctgacatat tcacggcgac cagcttaccc ctttgaattt
     4261 cagtgatgac ggaatgaaag atattgccgg acccgtcaat caagtcaaca gcatctccta
     4321 ccttttttct aaggcttttg gtcaaatgaa ggacctcatc accttctacc aaaatgacat
     4381 tatcattgat tcgtttcccc caaaatagct gcataatcac ttaaattacc tgcaaatctt
     4441 tagataaagc taaagaaact tttacctttg cgctcttcga aacacaacta aatattcaaa
     4501 acagtgtttt gacatggtaa aaataaaata aaagtaatta atggatgtaa tagtcatgat
     4561 cgcccaactg atattgagct tatcgatatt ggtaatctta cacgaattag ggcattttat
     4621 tccggccaag ttattcaaga caagggtaga aaaattttat ctgttttttg atccctggtt
     4681 ttcgttgttc aaatttaaaa aaggagatac agaatatggt gtaggatggt tacccttggg
     4741 agggtatgtt aaaatatcag gtatggtgga tgaaagcatg gacaaagagt ttatgaaaag
     4801 gccaccggag ccatgggagt tccgttctaa aaaagcctgg cagcgtctca taatcatgat
     4861 tggaggtgtg acggtcaatt ttattttagg ttttctgatc ttcggattga tattgtactt
     4921 ttatggtgag caaaaactgc cggtaagtga gatgcaatat ggaatagcca ccacccaatt
     4981 agctaaggat atcgggctga aggatggtga taaaatcctg caaataggaa ataatgattt
     5041 caaatatttc aattcaggag aactggcaaa ggatatcctg ctggataatg ccaaaacgat
     5101 ccgggtcaac agaaataatc tgataatgac cgtacatatt ccggacagtg cagtccagaa
     5161 aatacagtct aatcagaagg aagcaattgg catgattgca ccgcggatgc ctattatagt
     5221 cgatgaggtc atgcagggat ccggtggaga aaaggccgga ctccaaccta atgaccgtgt
     5281 catcagtgtc aacaatgttt caacacactt ttttcacgag atgaccaatg agttgcaagc
     5341 aaataaaggc aagaaggtta atataggtgt catcagagac ggcaatactg tgatgctgga
     5401 acaggtgcaa ctctctgatc agggcttggt tggcttcagt cctcaaccaa tatctcattt
     5461 ttataaattg gacaccatca aatactcctt gggaaaggct cttccggcag ggtttcgcaa
     5521 tggttacgac ttccttgcta cacagatctc cgcttttggc cagatgttca gtggaaagat
     5581 caaggcgtca gaatcattgg gaggatttgc aagtatcact aaactgttcc cgacagaatg
     5641 ggacagtcaa aagttttgga acatcactgc catattgtct ctgattttag ggttcatgaa
     5701 cctactgcct attccggcat tagatggagg atacattatg ttcctgcttt gggaagttat
     5761 tacaggccga aaggttaatg acaaggtaat ggagatagcg actacggcag gcatgatact
     5821 gcttatcgga ttactgttgt atgccaatgg gttggatgta attagagcat tgaagtaatg
     5881 gattattttg aatggtttgg cctggatagg aaattcaata ttgatcaagc cgggttgaga
     5941 aaagcatttc tgacaaaatc aatggaactt catcctgaca aaaccggtct gaatgaaaac
     6001 aaggctatgg aactgtcgga atataacaac aaagcttatg gaatactcaa ggattcctat
     6061 aagacgctgg actatattct tcacctggaa ggcattgttc tcgctgatga aaagtaccaa
     6121 ttacctcaag atttcctgat ggaaatgctt gaattgaata tggaaataga ggatgccgga
     6181 gagagtggag ataaagaaaa actgaataag atcaaaagcg atctggagac gaaaggaatt
     6241 gaagaagatg ctaacatgga aatacttaaa aatcaatatg atagtagaag tattgatcca
     6301 caaggctacg aaagtttgaa aaaattttat tttattcgca aatatctctt gcgaattgaa
     6361 gaaagtatta atacttttgc atcccattga agctaaaagc agtttctgag aataatttat
     6421 tgccgctgtg gcggaattgg tagacgcgct ggattcaaaa tcctgtggcc gtgaggtcgt
     6481 gtgggttcga ttcccaccgg cggtacaaat aaaaggagtt ttcggactcc tttttttgtt
     6541 ttaagacaca tcaaaaccaa cacaaaccca ccaaaaggat tccttcaata agtctcgtct
     6601 ttgttgtaaa atatttatca gctatagaac gatctgataa aatccgttag aataaggatt
     6661 ttagtacacc acatcttcaa tcaattcgta atgttgattg gcacaaaagc ttccggatta
     6721 gtcttattgg aatttaggcc atgttaatgt atatttttca ttgtctggct gtgacctcag
     6781 aaaaatctta cctttgaaaa atcattgcca gcgtacaaaa cgaaacactt cgaagtacct
     6841 tttataaaaa atccctttcg gccagaatta cccaagatgc tggatgatat catattgtgt
     6901 caggatgtga ttgacacctg caccatctct gacaagtacc gcggggatct ttttagagat
     6961 atacctacta agctctttga cagataatgc ctcttcaagg acaggaaatg gctcattcat
     7021 gatttcacct acggagacat tgtcaatatt atccggattg gacaacaggc ttgaaatgat
     7081 gtcactttca gtcaatgagc caactatttc cccctgatca tttacggtca cctgagagat
     7141 atcattttcc ttcatcatct gaaagacttc cctgagtttg ctgcctttct gtgctgtaat
     7201 cagtcctgac ttaggctttc tcttaacgag atcagaaagt cggagatcca tgctgataaa
     7261 accgcgatct ctcatccagt catcgttgaa gatctttgca acataacggc ttccatgatc
     7321 gtggaaaata ataaccacca catcatccgg cctgaatcta ttcttaacct gtaacaatcc
     7381 ggccatagcc gatcctgcgc tatacccaag taataggccc tcttcacgag ccaatctgcg
     7441 tgccatcaaa gctccatcct tatcagttac cttttcgaaa taatcgatca gattaaaatc
     7501 tacattctca ggcaagatat cttctcctat cccttcggtg atatagggat aaatctcatt
     7561 ttcgtcaaaa atgccggttt ccttatattt cttgaataca gaaccatagg tatctattcc
     7621 tatgatctct atatccggat tcttctcctt gagaaattta gcggtaccgc tcactgttcc
     7681 accggtaccc actcctacaa caagcttggt gatcttaccc tccgtttgtt cccaaatctc
     7741 cgggccggtt gtttcatagt gtgcttgaga attggccggg ttatcatatt gattaaacca
     7801 ataggaattt gggatttcct ggcttagtcg gcgagctaca gagtagtatg aacgcgggtc
     7861 agcagcctca acattggtcg gacagactat tacctcagct cccatggctt tgagcatatc
     7921 cattttctct ttggattgtt tatcacttgt ggtaaatatt gctttatacc ctttgacggc
     7981 tgcgactaag gccagtccca tgccggtatt gcccgaagta cactcaatga ttgtgccgcc
     8041 gggcttcaat ttcccttcgg actcagcgac ctcaaccatt tttaacgcca tccgatcctt
     8101 gatcgagtga ccggggttga atgtctctac cttagccaaa acagtgcaag gcaagtctga
     8161 tgtaatctta ttgatacgaa ccaatggtgt attgccaatg gtttccagga tatttttaaa
     8221 atacatgtga aagaatctaa attttcggca aaagtaggga tttattttca aagctgatgg
     8281 cagagcttga tgtgtgtttt tattaaatta cccaaaagaa aaattaaaaa tacacggaag
     8341 taattgctgc gtggaaacca tccttatacg gcacaaacaa atttaaactt aataagccaa
     8401 acgtagaaaa gctccgatca atcccaggtc cgatatcacc ctttgatata tgcaattttg
     8461 gcgaagaacc gggcatccct ggcgcctaat ttgatagcaa cttcaggaga aacaaccatc
     8521 ataacatcat ctgaatagct attgggggat actgttccca caactttggc gtacacagta
     8581 gagttgaaca tcggattggt tattttgatg atagaacctc gcggggcagt acgatgcaag
     8641 acaaacagac cattgctacc tttgagctct ttgtgccaga atgcaagtcc ctgctcaaaa
     8701 gccttagtcc cttcatatga ttcaaatttt tccctattgg aagcaggtgt cgattttgcc
     8761 tcctcactgg tcgtcttctc tacagcatcc tgtactgctt cttttgcaat ttcacgttct
     8821 ttagcggtgg atgtcgtacc caaagcattg ggcccgggct ttttcgtatc tgttttagca
     8881 ttgtctgcct ttgtgccgga ttttgggcgg cctttgtcag cgacgacatt ttcattgatt
     8941 ttattaaaga catcgagagg gatccagccc agcagaagta tttgcccaag ttttaaatca
     9001 ggggctgaca ttccattgat ctgcatcagc ttttccggtt ttataccaaa ataatttctg
     9061 gaaacactat ataatccttc tccgggactt acttcataaa caacaggagc aaagttttca
     9121 cgcttctttg gttcagactc aacccttatc aaacgagcac tgataggaac tctgattaac
     9181 tcatcgatct gtatccccct aaccttcagt accggattga gtgtcatcaa ttgagattgc
     9241 ttaatgccat agaacctgca cagagaatac atcgtttgct tcggcattac ccgatgataa
     9301 aagtatatta cacccagact atctctttga ataaatatag tatcattttt ggtcaatacg
     9361 gataatgagt ctcccgtcgc tccggccttt tgagagaaga gtgaaataac gaagacccat
     9421 gaaacaacat ataataactt tctcgatggc ataaccttaa gtgtatgaga ccttttaaag
     9481 gcaaatgtaa gtattttttg taaatgaaat atctgcctga aaagttcaat aaatcaggaa
     9541 atattttaat ataaaaaagc gaataggccg aagaaaaaac aggaagaatc atgaagtaaa
     9601 actaaaaatg ggctactctt acaacgaaaa tatttgtagc atggcgtata atcataacaa
     9661 atggatggta cagaaacgca gggaaaaccg gattggttat caagcttgcc tgacatacct
     9721 acaggactat ttaaccaatc tttttgctag aataagtcct tactttgggt agttccacgg
     9781 tgtagtttac cgattggaca aaagaatact gcgatcccgg taaccagact tcatttacga
     9841 gaaaccggtc aataatactc aatcaagaca tgatcaccat tttacatcaa ataaaatgaa
     9901 tccttcaaat caccatttat tttgaaaaaa gtcatcgcac gatcataacc ttcttgaccc
     9961 caaatacgga tggattgctt acccggacca gatatacgcc cggtttaacc gaatttaggg
    10021 gaattgtgat cgtagtttgg ccggataacc ggttgtcatg ataaacaatt ctacctgaaa
    10081 tatcagaaac ggtcactgaa acggtttcat aaaaaatcct ttccggcaat tgaatatgca
    10141 gttcactttc agcaggattc ggatagacgg taaattcaac atcctgtatc aaagtacctt
    10201 tgtctgaaag agtgatcccc ggattctcaa ccttcttatt gaggtagacc ttgtattctc
    10261 cttgtttcaa ggtaatttga cggagagaag tattaccttt gatgctatct ctacccatgt
    10321 aattataata ccattcgtcc gtaggaaagg taattgcctt actgccatcc gtgacatcaa
    10381 agtttcctat aactaccatc tttaagtcat tttgatccat tacgatgtat ttgaataagc
    10441 cattcaagtc ataatctatc tcaacgtcat caaacaaatc aggaaaacta tgtttcaggt
    10501 gtgttagccc cgccgtcact gcaaacaact ccagtctatt ctgttgatga taataatccc
    10561 aacgaatcgg cttgggatca acgcggcaat tattgttgac ggttccatcg ggacaatgat
    10621 tgatggagta atcataacct gtctcgccaa attgccaaag catcttaggc cccggaatag
    10681 tatagaagaa ggcagctgcc atccctaccc tcccgagtgc agttttcaga tcccgaacat
    10741 tatatccggc ggattgacgc ccttccttca atgccttgta cattacccgt tcttcatcat
    10801 ggctctccat ataggacacc aaatgcggct tacgccagcc tcgttgccgg aaatagctac
    10861 gattcaaact ggctgaattg cctttgacac tttcagcata ttcataattg atatttcccc
    10921 aaagcatcat gccatagtca gacaactcct tttcctcctg attatctgca aaatgctcaa
    10981 gaattacata agcctgagga tcaacttctc ttatctggtc tgaataatat ttcaaaattt
    11041 caatgcggga tggatcatac tggccccatg ctccgacatc attcggattg tttacctgtg
    11101 taaagccttt tgataagtca aagcgatacc catcaaaatg atattcacgg atccactccc
    11161 tgagtatctg actgacatac ctgcgtgttg cagcgctttg atgattaaag tcatagccga
    11221 cattgaatgg atgtttggca tcaggattaa accagggatt atcaattgcc ggtttgttct
    11281 gtgcagcatt ccaatacatt cggaccatag gattttgccc gaaagcatga ttgaacacaa
    11341 catccgcaat aacagcaaaa cccagaccat gagcggcatc tacaaacttc ttcaacttcg
    11401 atgcaccacc ataatatttg tctatggcat tatgtagaga aacattgtat ccccaactga
    11461 tattaccttc aaactctgag accggcataa gctctatcgc attgactccc aagcgcttca
    11521 gatatagcag ggtatctatt aaatcgtcaa aatcatggga tgccaggaaa tcccgtacca
    11581 gtaattcgta aatgaccaaa tcaccaacag caggtttgac aaaagcctca tcctgccagt
    11641 cataatcagc attcaatttc agatgagaca ccaatcccgt agtttttccc tccggatacg
    11701 acggcatgtc gggatatacg gcagccggga tatatttatc attgacatca tccaatatct
    11761 ccacactgag tggatcagga atcttcaaac catccccaaa ccaaatctga tagtctaaat
    11821 caccttgaag tcccacagta tcaatctctc cccaaagaaa ttgtccatcc ggtgactgct
    11881 tcatttgata ttccggtaat ggcgcccaat tactaaaatt acccaccaga aaggcgatat
    11941 tacgccgtgg cgcttccaaa aggacaatag ccttaccttc ctcaacctta ttgcacccca
    12001 atagaagtcc atctggcact tcactgattt caggatctcc tccaactgtc catacaaatg
    12061 aagtatccag accttcatta cctgcatgga ttccaatctc aaaacggaat gttcctacct
    12121 ggtcagcaat atactggtaa gacatggata catcatgagt tgttaccaca tcctgcccat
    12181 tgatagtaaa acgcaaatcg gccggtatag aagttaatac gctcaggttg agaacggaat
    12241 tcttagatac cagtcgactt tcaagacttg ggctctgtat taaatactgt aatccggcat
    12301 ttggatccgg aaaatcataa tagatgtctc catttgattc agtcttacct tcttttgatc
    12361 cattgaggtt acggaagaca aaagccagtt tcctgataat tgtactctca ggtatcccat
    12421 agaatgactt gatattgtat gtcagagaat acaggttgtc acttaccttg ttcattttaa
    12481 ccctggaatc gtctgtcccc caattgccga tgacatattt ccagtcggag ccgctgctgc
    12541 ttttatcggt tatcagcccg gcatgagcat aaacagtaca attgcaatcc ttaagcccct
    12601 gattaccctt agtagcgtca aaataaacag ttatggaaga gttaatattg ggaaatggcg
    12661 gatcaatagt cacctgtgca tgtaaaccaa ttgataagat caggccaact gagacaagca
    12721 gttgtttcat gtttttaaaa aattatttat gcaagataag gagaatagcg cattatgaaa
    12781 aatgaatagt gaagaaaacc tgaagtgtaa agaaagttat gtataactca agagccaaac
    12841 aatagaatta tacatccgga aagtaaacct ccggagtaga aaaaagaagg caaatcaagt
    12901 cccggagaag catacctaac caacaagagt atgatccctt tccaaaaatt gacttcaatc
    12961 catacaggaa gccggcttaa cagattgtaa ataaaccaac aatatataga tcattccaat
    13021 atatagcaac aaaacaaaat aaaatcaatc tgccttcgtt aaatcatgaa agacaattaa
    13081 aatctgcgca ataatttact tcagaaaatg caaagctcct aaaaagggag ttttcgtcct
    13141 caaaacacaa taattatctg tttttgaaaa ataaagtaaa aaaacatata agttattttt
    13201 taaatatatt acctttgctg attaaagttt ttaaatcaag ttctaaatcc ggattttatg
    13261 caatttttcc aattgtttct tctacagatt cctgatacca ctgctattca aagcatcgct
    13321 cttcctactc aaaaattgac ctttttcgaa ttgctgttga aaggtggagt gatcatgata
    13381 cctttggtga tactgtctat tatagccctg gggctgatta tagaaagatg gttgtctatc
    13441 aacaatctaa ccaaggtgga ggccaatttc ttcaacaaca ttaaatctat gctatccaat
    13501 ggtaatgtca aaggcgcgca ggattttgcc gagtccaaca acacctccat tggcaaagtc
    13561 atcggagaag gtataagata tattggctat ccctattctg aaatcgaaac ctatatgagt
    13621 gacactgcaa atatcgaact gggaaagatg gaaaacaggc tcaactatct tggaatcatt
    13681 gcaggggttg ctcctatgct tgggtttgtt ggtactatcg ccggtattat taaaatattt
    13741 tataatatat caattaccga taacatctcc ataggagtca tttccggagg actttacgaa
    13801 aaaatgatcg catcaggtac cggcttgata gtaggtatat ttgcctttat aggaaaccag
    13861 atgcttcaag caaaaatcga caggtatgcg ttaaaaatgc aggacatggt caaatccttc
    13921 cgctccgtac tgctacaaaa aggctaacgt atatttcttt ctgaaatatg attgttcatt
    13981 ctatgattaa ccccgaaaga aatatcagat gaaaataaat cgaaaaaggc acaaaccaca
    14041 tgtattcgtt gattcgctca gcgatatcat gttttttttg ttgctcttct tcctgattgt
    14101 ctcaacagtg gccaacccca atgtcgtgaa gatccttctc ccgaaatcgg gaaatacgga
    14161 agtattgagt aaaaaacaga ttactttgac cattacggcc gataaaagat actttatcga
    14221 caaacaagaa tataccatag accaattaga ggaggcgatt tcgcaagtca cagccaatgt
    14281 tacagagcca accatcatgt tgaggcttga taaatccctg actgtccagg atctgattga
    14341 cgtaatgtct atcggagcca agctgaagat caagatggta ttggctacag ataaaaaaag
    14401 ttgatttgcc ggctaagttt aaagtaggtc tgtgtgctta cgg
//
LOCUS       DAOQMC010000030        14412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1534_length_14412_cov_5.257644, whole genome shotgun sequence.
ACCESSION   DAOQMC010000030 DAOQMC010000000
VERSION     DAOQMC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14412
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1534_length_14412_cov_5.257644"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKV31_02235"
     CDS             complement(<1..604)
                     /locus_tag="PKV31_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91700.1"
                     /translation="MSGSDITGRSPLIRQVDLLSIEVLVDGAPLRDNYIVQSVNIHRK
                     INKISTAEFTFLDGDPTKVNFELEDEEVVVPGKSIVIKAGYHQDNNKIFEGVILKVGI
                     SIKPGGHSEITCFCTHKAQYLTIGRQNKYFIDKKDDEIISEIISDYGLPKSVESTKIK
                     HSQIIQYGCCDWDFILSRLEQNGLILLTDDDKVKVKKPDFN"
     gene            complement(614..1276)
                     /locus_tag="PKV31_02240"
     CDS             complement(614..1276)
                     /locus_tag="PKV31_02240"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91701.1"
                     /translation="MPGSSEKLKIYTYTDPNVPASGGSVGYEVELVPENISITYGLKF
                     KVDNPNTNKRVTKFEGYEDSTLKFDFILDATGVTGSQMVIKDEIEKLGKAIYNYVPSI
                     HKPHYLRIHYGSVNFICHLKKCTITYLLFKTDGTALRAKVSLEFDEFVLPANAESRVM
                     SSPDLSHIRFFTDSDNIFVFCDEIYANTNQAVQIARINNIVNFRNIPPGTQVLFPPFE
                     RL"
     gene            complement(1266..1439)
                     /locus_tag="PKV31_02245"
     CDS             complement(1266..1439)
                     /locus_tag="PKV31_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91702.1"
                     /translation="MPVEIKELKVTIEVRPKSLDVREIEKLVLDLFEKERSKVENNIT
                     LKALKKEGIKNAR"
     gene            complement(1446..1940)
                     /locus_tag="PKV31_02250"
     CDS             complement(1446..1940)
                     /locus_tag="PKV31_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNB91703.1"
                     /translation="MANGGDWDQYIPLSFQFSVTIDGETLSFSEASGLHSEMVVEELR
                     VGGRNDRVYSLPVRVKNTNLVLKRAFNTNIKPSNKVERNKPLYTDWINKVLYEDGNLN
                     VRLELKNIVVKLLDPKGNELMSWEIEGAYPVKWSISNLNATENNVAVETIEFAYQSVR
                     LKVD"
     gene            complement(2209..2661)
                     /locus_tag="PKV31_02255"
     CDS             complement(2209..2661)
                     /locus_tag="PKV31_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNB91704.1"
                     /translation="MADFENQWPVAKYHFRVTIDGEQISFQEASGLQAETTPIEYRHG
                     DSEAFHVIKQAGLIKTSNLVLKKGVFESDSRLLDIFNKIYDKSYYTQDDRVDIIVELL
                     DETASTVMTWNITKAFPIKFTGTDLKSTANEVSIETIEFAYEEIKTEL"
     gene            complement(2691..4328)
                     /locus_tag="PKV31_02260"
     CDS             complement(2691..4328)
                     /locus_tag="PKV31_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HNB91705.1"
                     /translation="MMQYATPGVYIKERNAFTNSVVAVPTAVPAFIGYTEKAVNGTTS
                     LKNVPTRITSLAEYVTVFGGPPRIQFNIKGKSNLDFDLKVEKQTQYLLFNSIRLFFAN
                     GGGTCYIVSIGDYKNGVNAKDFNDTENGGGLHTLLAEQEPTLICAPDAVLLPKADCMT
                     FYQEVLKHCGYTMKSRFGLFDVYDGDQPRSYDKADIITNFREGVGNNFLNYGASYYPW
                     LNTTIVTGSEISFKNISNIPDLVKLLTAEAEQIYLQAVSAEDEVAEAETGGDDKAKGD
                     DKAKKAPAPSVKPAKVYDERSMRLFEAAKFEIEKLKDTAAESLVVDNTLKTISPLYKS
                     ILAAIKDKVNVLPAAAAMAGIYTMVDNSIGVHKAPANVSLIAVTSPTVSITADNQADL
                     NLPINGKAVNAIRTFIGKGILVWGARTLDGNSQDWRYINVRRTMIMLEQSIKNAVENY
                     VFDPNTAQTWVKIRTSIENFLTLAWRTGALVGDTTADAFEVHVGLGVTMTPQDILEGL
                     LRVLVRVAVSRPAEFIEITFEQKLQETGGFVTEPMGA"
     gene            complement(4914..6011)
                     /locus_tag="PKV31_02265"
     CDS             complement(4914..6011)
                     /locus_tag="PKV31_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91706.1"
                     /translation="MLSPGQYTSKWSKWISLKLRHKEKNIKVIDDFELLPSSETELFL
                     TRINAVSRKHNDTYAIFVNDLRSLSLIPTLTNIPEKVNFEGMLNNNNFILHSDTNHLP
                     DRLFVNYINNFKSFRIDEMTLSLHKSDFLKASEMEILELATPVFSVTLPRMESNVPVA
                     FLDARLQKIANVKHEITALSDENSQLACDFTNSSKGACTIQVNGKDVKKIYISGRKRL
                     HFAAEIFLKPDAKSESLMQPERFVPVNYKITFDIKKTYWRYILISKSEERNVYDIKDI
                     KFELNGEEVPTEAIKKTELVNGVRCLHVTFRDEMALRLNTFNHEIMKMKINSVNKWTP
                     STIRAPIPTYKNVQRTERSSGKSYSLLYVYI"
     gene            complement(6017..6529)
                     /locus_tag="PKV31_02270"
     CDS             complement(6017..6529)
                     /locus_tag="PKV31_02270"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HNB91707.1"
                     /translation="MIHEVIPIIAGELKDYLETKFGSYDDVVIISNLINQDGSIAIRE
                     DNKIVVTLLSIERDGSNLNFGGGMIRTDMPLHINLYMLFSAYFNDYAESLKFLSGVIG
                     FFQGNPQFIIDDNTIKAELFHIDFRELSNLWTCLGAKIVPHVMFKFRTLNMDEERIRE
                     EIPPITGITI"
     gene            complement(6526..6822)
                     /locus_tag="PKV31_02275"
     CDS             complement(6526..6822)
                     /locus_tag="PKV31_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91708.1"
                     /translation="MFRRLYNRIFLPAIRFILPGITPRKLILAIILSVIFSYFFYLFV
                     TTRVAGKEWKIWLWGLFSVFFGVLFTRAVANQIKNYLSRRKEIIELMATNKSKK"
     gene            complement(6829..8397)
                     /locus_tag="PKV31_02280"
     CDS             complement(6829..8397)
                     /locus_tag="PKV31_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91709.1"
                     /translation="MTIIKSYRLIILISITALVSSQLNAQSFFNRSWAKKFKNLFSSS
                     EKLPEPDIYVLKKQSLVMVDEPIWLIKNKSYEKFYTNLMTSVVIGNYDINLLNGEARN
                     DEGTTWFDDGFSGVRDSLSNVYSKLLEKNKHLEFYLLVTSYGDIIDQSYLSSIYNSFL
                     NEKESQNKMIDNLRTIIRNLQLKLKIQEHQIGIIFDIQNIPKDGIQSEKVVNFYTYIK
                     EQLFPKGSLSKIGLKLPNLVEENSIYYDGNTLGKIGPLFDFVIYKNYCLDFEKVASPE
                     YLEALTSHNTANLRKGIDSLSRMGINKHDIIIEFAYTGLLLDRNMSGFSLNRILSYNQ
                     IDKLSASNIAARSGNVGESEFTSLNIIDKKDETYSRVIYEDHQVRLRKYKLIDSLGAR
                     GICLHGIGYTGNMRDQQRSALWLAVAEVYGWIPDKLYWYIIGTILGFMSLGFPYSVYK
                     FWQVRNVLAKFRRHLIPVGGIWFLLTFGFFFCFNFIPRNTLGVFLGLGFVLFFVLLIL
                     FRIYILRIIRLFKK"
     gene            complement(8394..9887)
                     /locus_tag="PKV31_02285"
     CDS             complement(8394..9887)
                     /locus_tag="PKV31_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91710.1"
                     /translation="MLVLTSLSFQTVSPDRISVRNLGISNKEEVEIFLNFSKDDIVSF
                     NFKSSDEQNVSDKSMTYSLSKIPISLDNSVVREKQVSEASKPVKILSDGQYLLTVRTS
                     QKGVQFFNLNLDKMNKPWNSNAPLIKIHASIMDRPGRNQKKLRLSYSVQEKSSINIIG
                     AGKSADIFTLEENSFRINTTLDKGINISEKSKPEILIYLDEENLKKKNLWQIVESLFK
                     KGRYPNLKNLAEISFGVVQNSTAESSKDDLVFSKPSSNQTGTDQKGQGGQSRGSQSGT
                     GNSFAGTNNSGMPLSRIPQDTANLFDTPEKITIGSNFQRIDSSMYLSPVFDYSADQKR
                     KCKSISKMIGVGEDDMLGFWVGISTRQLLEYNRQREVYKGKMIGYWSPLMQYADVLYI
                     RKMMPMAMNIETRHLPLYAFTTMLDNIEIAIVDEQNKELFEKQGDYKSYIDSPYNGLY
                     KTSGQMFGFIKLPDESTELYLCSCNRNKYNGVRLYTIFETFSKNELQ"
     gene            complement(9935..12229)
                     /locus_tag="PKV31_02290"
     CDS             complement(9935..12229)
                     /locus_tag="PKV31_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91711.1"
                     /translation="MNRQTHKYPLILICLFLWVTLDAQCIKDAFVRIDTIELPAEIKN
                     RLQIKITRFYQAYKAQSRVSDFTIVENYHGTDRYLKIMDIAQVRDTMNVLVHIDHISD
                     QNNINLIIDAFNRLNQSEKSTVFLPTIIGDSLNFYKIKKFNSMNNVLAVNDFKQKRYS
                     LDQRAIRGNLNTLHSTSCYIWLIDSKNIYNIHATIGKIFPNENKNKQAAPLFVPYLIS
                     DIPTSDYSQLRDIMQKVPCSIDSVIIDINLKKFHRHLKDIKSANFPVSYDVFFDPAKP
                     FYLGENRQIVLTWRSNLGKDLIVLRDTATYSFGSSDFPVNVKELKKDSLFWFSSAFIG
                     ILFLLVLFFILSILYPKIEERKFFKEYVSPYKPVNNIIKLDNITNEPIKEGTLVVQRC
                     KEVVPFYIWKSLGNQCPSYPECMDILGCDGCGKSDINYKFFSKSGSLKKYNWLFFGAC
                     GGFFAWVFTLLTIPVFHRIHLADLFQGLYEKKELINSSLMFFKENVINQSAVGFGIAL
                     GLAGVLTMVDYFSSIVRQHIIINILKIVDLAIVAALFFGLVNAFSYRFDITPFFSGLI
                     SWTGFALLFAITLSLKSATIRFKRALIASLMGVLITYLFFYISSSIVNIGYENTTIFR
                     QIIMESLKILRFQILGMLIGYITMNVISRQEDYELELQSPDSVRGKVIPLTKALNTNN
                     AITIGTSPKNVIRIKWIDAGAEEYHASINYSAEGPVLETFAEVIVNREYLPKGTRYIL
                     QDRDMIQLGRHSSTLFLFIKKYHS"
     gene            complement(12369..13292)
                     /locus_tag="PKV31_02295"
     CDS             complement(12369..13292)
                     /locus_tag="PKV31_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB91712.1"
                     /translation="MYYLKLPYIQKLLVLLAGWVLLCTIYYFIFMQERLEHEIIKKID
                     VDMDRCLTHRLEPAPLEIVDETGGYSIKLRGNIHCKWKSAKPEFYCRDQFRSNFTNLK
                     HYLEDHPDKALRINGNYSKEEYNQTELGDLGYTRAEVLRQYLIDSMGYRIEMINIGTG
                     VKETNDLIVLDKMLYRPYVLTIEDIITFTANSDLEYVQPIYFDFDFRSLIPSDSLLNY
                     LGNIASKYKNKKSYLLTITGHTDYMGSNEYNMKLGMDRAKFIEALIQEKFNYQNTVCR
                     SEGEENPKYDNSTSQGRRMNRRVDLQIEKTE"
     gene            complement(13294..14253)
                     /locus_tag="PKV31_02300"
     CDS             complement(13294..14253)
                     /locus_tag="PKV31_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91713.1"
                     /translation="MPEITYSSIEHLIQDVTTTGHQVQCSFYHLNGELISSNASIPHN
                     NSTMNRVTRVVKNQMINRARATASQSIFSLLGGGILGRAGSTIIRASVPANSSFSFEP
                     SQDDINDAVVKAFEKISTRYRYDENSGNWTPTDSPNSRSGKPTPKPNSPTKEVKNRPE
                     PHKEPGLNRLEKEVLARVLVEICDADGNLSGEEAEFLNKILPKELGSVEKIRNMDYIS
                     PLELKALSENAKKQIVKCAFILSYSDLQPHETETGIIQEYATQMGLSSQVLRQMEKEA
                     QKELVMTMIDENTTRAEVYEIGRQINLSDEESEMILIHKKESM"
BASE COUNT         4096 a         2873 c         2684 g         4759 t
ORIGIN      
        1 aattaaaatc aggttttttt acctttactt tatcgtcatc ggtaagaagt atcaatccgt
       61 tttgctcgag ccttgataag ataaaatccc aatcgcaaca tccatattgt atgatctgac
      121 tatgctttat cttggtgctt tcaacagact ttggcaaccc ataatcactg atgatttctg
      181 atataatctc atcatctttt ttgtctatga aatatttatt ttgacggccg attgtcaaat
      241 actgagcttt atgggtacag aagcaggtta tctcagaatg accacccggt tttattgata
      301 tacctacttt tagaattacc ccttcaaaaa tcttgttgtt atcctgatga tagccggcct
      361 tgattactat actttttccg gggacgacaa cttcttcgtc ttccagttcg aaattgactt
      421 tggtggggtc tccatccagg aaagtaaact cagcagttga aattttattt attttccgat
      481 ggatgtttac cgattgcacg atataattat cccgtaaagg tgctccatcg accagtacct
      541 ctatggagag caggtcaacc tgtcttatca atggcgaacg tcctgttata tcacttccac
      601 tcatagctta aggttataat ctttcaaagg gtggaaatag gacttgtgtt cccggaggga
      661 tatttctgaa attaacgata ttgttgatcc ttgcaatttg cactgcctgg tttgtattgg
      721 catatatttc atcacaaaaa acaaagatat tatcgctgtc ggtaaagaat cttatatgag
      781 acaaatcagg agaagacata acccgacttt ccgcattggc aggcagcacg aactcatcaa
      841 actccaggct gacttttgct ctaagggcgg tgccatccgt tttgaataaa agataagtta
      901 tcgtacactt tttaagatga caaataaaat tgactgatcc gtaatgtatt cttaagtaat
      961 gtggtttgtg gatgctcgga acataattgt atattgcttt gcctagtttt tcaatttcat
     1021 cttttatgac catctgactt cctgtaacgc ctgtagcatc caggataaag tcaaatttga
     1081 gagtactatc ctcatatcct tcaaattttg taactctctt atttgtattt ggattatcta
     1141 ccttgaattt tagtccatag gtgattgaga tattttccgg caccaactca acttcatacc
     1201 ccacggagcc accactggcg gggacattcg ggtcagtata agtatagatc ttcaattttt
     1261 cactactacc tggcattttt tattccttct ttttttaagg ctttcagggt gatgttgttt
     1321 tcaactttcg acctttcttt ctcaaacaaa tccagcacga gcttttcaat ctccctgaca
     1381 tccaaacttt tgggccttac ttcaatagta actttcaact ctttaatttc aacaggcatc
     1441 tcagattaat ctactttcag gcggacggat tgataagcaa attcaatggt ttcgacagct
     1501 acgttatttt ctgtcgcgtt taagttggag attgaccatt tcacgggata agctccttct
     1561 atttcccaac tcatcaattc gtttcccttt ggatccaata attttactac aatattcttt
     1621 aattcaagcc gcacgtttag attaccatct tcatataata ctttatttat ccaatcggta
     1681 taaagtggtt tgttcctttc aactttgtta ctgggtttga tatttgtatt aaaagcccgc
     1741 tttaacacaa gattggtatt cttgaccctc accgggaggc tgtacactct gtcattcctg
     1801 cccccaaccc ttaactcttc aacgaccatt tcggaatgaa gtccacttgc ctctgaaaaa
     1861 ctcagggttt caccatcgat tgtcaccgaa aactgaaatg acaacggaat atactgatcc
     1921 caatctcctc catttgccat gattacttat ttactctttc tgtaaagatt tggttacagt
     1981 ttatttaaca catactgatt gaagactatc tggatgttgg ggcagtttca atatttattt
     2041 cctattcctg acttgataaa agtaaataat cgggacatgt tgatccgcga ttgatttata
     2101 tcaaagtcat aagaaaatgg aaggttccta tccactccca attataacag ccccaatcca
     2161 aatacaaatc aaccttgaaa gaaacttcca aggttgattt agtaaatatt acaactcagt
     2221 cttgatttcc tcgtaagcaa attcgatggt ttcaattgaa acctcatttg ctgtggactt
     2281 caagtctgtt ccggtaaatt tgattgggaa tgctttagta atattccaag tcataacagt
     2341 ggaagcagtt tcgtcgagca attccacaat gatatctacc ctgtcatcct gtgtatagta
     2401 actcttgtcg taaattttgt tgaatatatc caacaatcga ctatctgatt caaaaacgcc
     2461 tttcttaagg accagattac ttgttttaat caatccggct tgcttaatga cgtgaaatgc
     2521 ctcactatcc ccatgcctat attcaattgg ggttgtttca gcctgcaggc ctgatgcttc
     2581 ctgaaagctt atttgttcgc catctatggt aacccgaaaa tgatacttcg ctaccggcca
     2641 ttggttttca aaatctgcca ttttatttat tttttaggat tatcaaaaaa ttatgcaccc
     2701 attggttctg taacaaaacc tccggtctcc tgcagcttct gttcgaatgt aatttctatg
     2761 aattctgccg gtctgctaac tgctactctt accaataccc tgagaagtcc ttcgaggata
     2821 tcttgaggag tcatggtgac ccctaagcca acatgtactt caaaagcgtc tgccgtagtg
     2881 tctcctacca atgcacctgt tctccatgcc aaggtcagga agttctctat ggatgttctg
     2941 attttaaccc aggtctgggc tgtgtttgga tcgaaaacat aattttctac tgcattctta
     3001 atggattgct cgagcatgat catagtcctt ctgacgttga tatatcgcca gtcttggctg
     3061 ttaccgtcaa gtgttctcgc tccccacacc aagattcctt ttcctatgaa tgtccttatt
     3121 gcattgactg ccttaccatt gatggggagg ttaaggtctg cctgattgtc cgctgttata
     3181 gacactgttg gtgatgtaac tgctatcagg gaaacattgg caggggcctt atgtacacca
     3241 attgaattat ctaccatggt ataaattcct gccatggctg ctgctgccgg cagcacattg
     3301 accttgtcct tgatggcagc cagaattgat ttgtacaacg gactgattgt tttcagcgta
     3361 ttatccacca ccaggctttc tgctgcggta tccttaagtt tttcaatttc gaatttggca
     3421 gcttcaaata atctcatgga gcgctcatca taaaccttgg ccggtttgac ggaaggtgca
     3481 ggtgcttttt tagccttatc atctccttta gccttatcat caccacctgt ttcagcttct
     3541 gcaacctcat cttcagcact caccgcctgc agatatattt gctcagcctc tgcagttaac
     3601 agtttaacaa gatcggggat attgcttata tttttgaatg aaatctcaga accggtgacg
     3661 attgttgtat tcaaccaggg ataataggat gctccataat tgaggaaatt gttgcctact
     3721 ccttcgcgga agttggttat gatatctgcc ttatcatagg aacgaggctg gtctccatca
     3781 tacacatcga ataacccaaa tcttgacttc atggtatatc cgcaatgctt cagtacttct
     3841 tgatagaaag tcatacaatc ggcttttggc agaagtacag catccggtgc acaaatcagt
     3901 gtgggttctt gttctgcaag taacgtatgg aggccgccgc cgttttccgt gtcattgaaa
     3961 tcttttgcat tgactccatt cttgtaatct ccgatggaaa ctatataaca agtacctcca
     4021 ccattggcaa aaaagagcct gatgctattg aataataaat attgagtctg tttttcaacc
     4081 ttgagatcaa aatccaaatt gcttttacct ttgatattaa attgaatcct cggtggtccg
     4141 ccaaagacgg ttacatactc tgccagagaa gtaatccgcg taggcacgtt tttgagcgat
     4201 gtagttccat ttacggcttt ttcagtatag ccaataaatg ctggtacggc tgttgggaca
     4261 gccactaccg aattggtgaa ggcgtttctt tccttaatgt aaacacccgg cgttgcatat
     4321 tgcatcataa tcaaaagttt ttaaatttat tatttaccta tttgtataat gagcttaatt
     4381 aaaaattatt gaattgtggg ttaaaatatt tttaaacaat cccgtttgtc attgtttaaa
     4441 taatatgata gttgtggtga atttatctta agacctttag tttaaatccc gcatgaaata
     4501 actaatgtaa ctaaatttgc aaatatttgt tctgtactat ttggtataaa caaatattaa
     4561 ttcagtctgt aaatttagtt attaaactaa ataaaaacaa ttttttttcc tgatatttta
     4621 aatatttaag aaaaaagatt cgtggggctt tgaagattaa gggacaaaaa ttaaaagctg
     4681 aaaatcaata cgatagtaaa aaaatattcc gttttgccat ataagcaaaa aactaattta
     4741 taataaattg tatatcaaca ttgttgttgt ttggttgtta ttttgctttg aattcaaagg
     4801 gaagtggacg gatcactaaa tatcttgcag ttgacgctca gaaatatcca cctttacctg
     4861 gaggtgttat ggcaaccatt tcctatctca aacgcgattt ttgagttgtg acgtcaaatg
     4921 tacacataca acagtgaata agatttgccc gatgatcttt ccgtacgctg tacattttta
     4981 taggtaggta ttggggcacg gatagtagag ggggtccatt tattgacgct gttaatcttc
     5041 atcttcatga tttcatgatt gaatgtattc agccttaatg ccatttcgtc cctgaaagtc
     5101 acatgtagac accgcacacc attgactaat tctgtttttt taatagcttc tgttggaact
     5161 tcctcaccat tcaattcaaa tttaatatcc ttaatgtcgt acacattcct ttcttccgat
     5221 ttggagatta agatatagcg ccagtatgtc tttttgatat caaaggtaat cttgtaattg
     5281 acaggtacaa atctttcagg ttgcatcaat gattctgact tggcgtccgg cttaagaaat
     5341 atttctgcgg caaagtgtaa tcgctttctc cccgaaatat agatcttctt tacgtccttc
     5401 ccattgacct gaatggtaca tgcgcccttc gagctattgg taaaatcaca tgccaattga
     5461 gaattttcat cagacaacgc cgttatctcg tgtttgacat tagcgatttt ctggagtcgt
     5521 gcatcaagaa atgcaaccgg cacattactt tccattcggg gcaaggtcac actgaacacc
     5581 ggggtggcca gttcaagtat ctccatttca gaagctttaa gaaaatcact tttatgaagt
     5641 gacagggtca tttcatctat ccggaatgac ttgaaattat tgatataatt gacaaaaagt
     5701 cggtccggaa gatggttggt atcactgtgc agaataaaat tattgttgtt gagcatccct
     5761 tcgaaattga ctttttcggg gatattagta agcgtaggga ttaaggacaa tgaccgtaaa
     5821 tcattgacga aaatggcata ggtatcattg tgctttctgg atactgcatt gattctggtt
     5881 aagaacagct ctgtttctga acttggaagt aattcgaagt catcaatgac tttgatgttt
     5941 ttctccttgt gcctaagttt caaggaaatc catttggacc atttgctggt atattgaccc
     6001 ggtgacaaca tgacaattag attgttattc ctgtaattgg tggtatttct tctcttatcc
     6061 tttcttcatc catattgagt gtcctgaatt taaacataac atgcggaact atttttgctc
     6121 cgaggcatgt ccacaggttg ctcaactctc tgaaatctat atgaaacagt tcggctttga
     6181 tggtattgtc atcgataata aattgcggat tcccctgaaa aaaaccgatt actccggaca
     6241 ggaatttcag agattccgca tagtcgttga aatacgcact aaacaacata tagaggttga
     6301 tatgtaatgg catatccgtg cgtatcatcc ctcccccaaa attcaggtta gaaccatctc
     6361 gttctatact caacagagta actacaattt tattatcttc cctgattgct atgctaccat
     6421 cctgattgat caaattggaa atgattacta catcatcgta ggaaccgaac tttgtctcaa
     6481 gataatcttt cagttctcct gcaattattg gtattacttc gtgaatcatt ttttgctttt
     6541 attggttgcc attagttcta ttatttcttt tcttctggat agatagttct tgatctgatt
     6601 tgctaccgcc ctggtaaaga ggacgccgaa aaaaaccgaa aaaagacccc agagccatat
     6661 tttccattct ttgccggcta ctcgtgttgt gacaaagaga tagaaaaaat aggaaaaaat
     6721 cacactgaga atgattgcaa gaatgagttt ccgaggtgtt atcccgggaa gtataaaacg
     6781 gattgccggt aaaaaaattc tattgtacag ccttcggaac atctctcatt attttttaaa
     6841 caaacgaatg attctaagta tatatatcct gaacagaatc agtaatacaa aaaacaggac
     6901 gaagcctaat cccaaaaaga cacctaaggt attcctgggt ataaaattaa aacaaaaaaa
     6961 gaagccgaaa gtaagcagga accaaatacc gccaaccgga atcaggtgcc ttctaaactt
     7021 agccaataca ttccgtactt gccagaactt atatacagaa taaggaaatc caagtgacat
     7081 aaatcccagg atagtcccaa ttatatacca gtataatttg tcgggtatcc agccatagac
     7141 ttcggctact gccagccaca aagcacttct ttgctgatcc cgcatattgc ctgtatatcc
     7201 tataccatgc aggcatatac cccgggcgcc cagggagtcg atgagtttgt atttcctaag
     7261 cctgacctga tggtcctcgt aaattactct ggaataggtt tcgtcttttt tgtcgatgat
     7321 attcaatgat gtaaattcag actctccaac attgcctgat cttgctgcga tattgctggc
     7381 tgaaagttta tcaatctgat tataggataa aatcctgttc agactaaatc ctgacatgtt
     7441 tcggtcaagt aataatcctg tataggcaaa ttcgatgata atatcgtgtt tgttaatacc
     7501 catcctggat agagagtcga ttcccttgcg gaggttggcg gtattgtggc tcgttagagc
     7561 ttccaggtat tcgggggatg ccactttttc aaaatcgagg caataatttt tataaatgac
     7621 aaaatcaaaa agcggtccta tcttcccaag ggtatttccg tcataataaa ttgaattttc
     7681 ttcaaccaga ttgggcaatt ttagtccaat cttggaaagg ctgccttttg ggaagagctg
     7741 ttctttaata tatgtgtaaa aattaacaac cttttccgac tgaataccat ccttaggaat
     7801 attctgtata tcaaagatta tacctatttg atgctcctga atcttaagct taagttgaag
     7861 gttcctgata attgttctga ggttgtcaat cattttattt tgactttcct tctcattcaa
     7921 aaaagaatta taaatactgg atagataact ttgatcgatg atatcaccat aagaggtaac
     7981 caggagataa aattccaaat gcttgttttt ttcaagtaat ttggaataca cattggacaa
     8041 ggagtctctt acgccggaaa agccatcgtc aaaccaggtg gtgccttcat cattccgtgc
     8101 ttcgccatta agaaggttga tgtcataatt gccgatgact acactggtca tcagattggt
     8161 ataaaacttt tcgtaagatt tgtttttaat aagccagata ggctcatcga ccattaccag
     8221 actttgtttc ttaagaacat agatatccgg ctccggcaat ttttcactgc ttgaaaacaa
     8281 gttcttgaat tttttagccc agctccggtt aaaaaaagac tgagcattga gctgacttga
     8341 taccagggct gtgatagata tcagaatgat tagtctatat gatttaatga ttgtcactga
     8401 agctcatttt ttgaaaaggt ttcaaagatg gtataaagcc ttaccccatt atacttgttt
     8461 ctattgcagg agcacagata caactcggta ctttcatctg gcaatttaat aaacccgaac
     8521 atctgaccgg aagtcttgta cagaccattg tacggagagt ctatatatga tttgtaatcg
     8581 ccctgctttt caaagagttc tttgttctgc tcatccacga ttgcaatctc aatattgtcc
     8641 agcattgttg taaaagcata taatggaagg tgtcttgtct caatattcat agccataggc
     8701 atcattttcc ttatatatag tacatcggca tattgcatca atggactcca gtagccgatc
     8761 attttccctt tatagacctc tcgctggcgg ttatattcca ataattgtcg ggttgagatc
     8821 cctacccaga atccaagcat atcatcctca cctactccga tcattttcga aatagatttg
     8881 cattttcttt tctgatcagc agagtaatca aaaacagggg aaagatacat cgaagagtca
     8941 atcctctgga agttagagcc gatagtgatt ttctccggtg tatcgaagag attggcagtg
     9001 tcttgtggaa tcctgcttaa cggcataccg gaattgttgg ttcccgcaaa tgaattgccg
     9061 gttcccgatt ggctgcctct tgattgtcct ccctgtcctt tctgatctgt ccctgtttga
     9121 tttgaagaag gcttgctaaa tacaaggtca tccttggaag attcagcagt ggaattttgg
     9181 acaacgccga agctgatttc tgccagattc ttaagattgg gataccttcc ttttttgaat
     9241 aaagattcca ctatttgcca aagattcttc ttcttcaggt tttcttcatc gaggtaaatc
     9301 aatatctccg gttttgactt ttcagagatg ttgattcctt tatccagggt tgtattgatt
     9361 ctaaaagaat tctcttccag tgtgaagata tctgcggact tgcctgcacc tatgatattg
     9421 atggaagact tctcctgtac tgaataggat aaccttagtt tcttttgatt tctgcccggt
     9481 ctgtccatga tggatgcatg aatcttaatc aaaggagcat tgctattcca gggcttattc
     9541 attttatcaa gattcagatt gaaaaattga acacccttct gactagtccg gacagttaat
     9601 aaatattgac cgtcagataa tatcttgact ggtttggagg cctcagatac ttgtttttct
     9661 ctgacaaccg aattgtccag agatatagga attttgctga gagaataggt catgctttta
     9721 tccgacacat tttgttcatc tgatgattta aaattgaacg agacaatatc gtccttgctg
     9781 aagtttagaa atatttcgac ttcctccttg ttggagatgc ccagattcct gaccgaaatc
     9841 ctgtctggac tcacagtttg aaatgacagt gatgtaagca caagcatggt taaaattatt
     9901 aacctcccgt aaaacgattg tgtgtaaagg aacatcatga atgatatttt ttaataaaaa
     9961 ggaatagcgt tgaactatgt cgacccaatt gaatcatatc tctgtcctgt aaaatatagc
    10021 gggtgccttt aggtagatat tccctgttga ctatcacctc agcaaaggtc tcaagtacag
    10081 gcccttctgc cgaataattg atggaagcat gatattcttc agcacctgca tcaatccact
    10141 tgattctgat gacatttttt ggagaagttc cgatagtgat ggcattgttg gtattcagtg
    10201 ctttcgtcag cgggataact ttacccctta ccgagtcagg cgactgtagc tccaattcat
    10261 aatcttcctg ccttgatatt acattcatcg tgatataccc aatcaacatg ccaagtattt
    10321 ggaatctgag aatttttaga gattccataa tgatctgacg aaatatagtt gtgttttcat
    10381 accctatgtt gacaatcgag gagctgatat aaaagaaaag gtatgttata agaacaccca
    10441 tcaaggaagc gatcaaagct cttttgaatc tgattgttgc acttttcagc gaaagggtga
    10501 tggcaaataa caaagcaaat ccggtccagc taataaggcc ggaaaagaaa ggcgtgatgt
    10561 cgaatcggta ggaaaatgca ttgaccaatc caaagaacaa tgcagctact atggcaaggt
    10621 cgacaatttt gagtatgttg ataattatgt gttgtctcac aatactggaa aagtaatcca
    10681 ccatggttaa tacacctgcc aggccaagag caatgccgaa accgactgcc gattgattga
    10741 tgacattttc cttgaaaaac atcagactgg agttgataag ctcctttttt tcatataagc
    10801 cctgaaacag atctgccaaa tgaatccggt gaaacacagg tatggtgagc aaggtaaata
    10861 cccaggcgaa aaaaccacca caggctccaa agaaaagcca gttatacttt ttcaaactgc
    10921 ctgacttgga gaagaattta taattgatat cagacttgcc acaaccatca caccccagaa
    10981 tgtccataca ttcaggatag gaagggcatt gattgccgag gcttttccaa atataaaaag
    11041 gcaccacttc cttacacctc tgaaccacga gagtaccctc ttttattggt tcattagtta
    11101 tattgtccag tttgatgatg ttattcaccg gcttataggg tgaaacatat tctttaaaaa
    11161 atttcctttc ttcaatctta ggataaagta tactgagtat gaagaacagt accaataaaa
    11221 acagaatgcc aataaatgca cttgaaaacc agaaaagcga atcttttttc aattccttga
    11281 cattgaccgg aaaatcgctg ctgccaaatg agtaagtggc agtatcccgg aggacaatca
    11341 ggtctttccc caaatttgat ctccaggtta gtacgatctg tctgttttca cccaaataaa
    11401 aaggttttgc tgggtcaaag aaaacatcat aggagaccgg gaaattcgcg gatttgatat
    11461 cttttagatg tctatggaat tttttcagat taatatcgat gatgacacta tcaatcgagc
    11521 atggcacctt ctgcataatg tccctcaatt gagaataatc cgaagtcgga atgtcggaaa
    11581 taagataagg gacaaaaagg ggtgccgctt gtttgttctt gttttcgttt ggaaaaattt
    11641 ttccgatagt agcgtgtata ttgtaaatat tctttgaatc aatgagccat atatagcagg
    11701 aagtagaatg caaagtgttt aggttgcctc ttattgccct ttggtcaagt gagtatcgtt
    11761 tctgtttaaa gtcattcact gccagaacat tgttcatgga attgaatttt ttaatcttgt
    11821 agaaattcag actatcacct atgatggtag gtaaaaagac agtagatttt tccgattgat
    11881 ttaatctatt gaaggcatct attattaggt taatattatt ctggtcgctt atatggtcga
    11941 tgtgcacaag gacattcatt gtatcacgca cttgagcaat atccatgatc ttgagatacc
    12001 gatccgtacc atgatagttt tccacaatgg taaaatcgct taccctgctt tgtgccttat
    12061 aggcctgata gaatcttgta atttttattt gaaggcgatt tttaatttca gcgggtaatt
    12121 ctattgtatc aatcctgaca aatgcatcct taatgcactg ggcgtccagg gttacccata
    12181 gaaaaaggca tatcaggatt aaaggatatt tgtgggtttg acgattcatc ctacttttct
    12241 tctatgcatt tttataaaat tgtcctgact tttcccatat tttacctttc caatgtaaat
    12301 tccggcaaaa agtccaataa tggtagtgcc cagaagcata atcagcaatt ctaaatatgt
    12361 aatcattttt attccgtttt ttcaatttgt aaatctaccc ttctgttcat cctgcggcct
    12421 tgacttgtac tgttgtcata tttagggttt tcttcgccct cactcctgca gacagtgttt
    12481 tggtagttga atttttcctg tattaaggct tcaataaatt tagccctatc catgccgagt
    12541 ttcatattgt attcattact gcccatatag tcggtatgac cagtaatggt caataggtag
    12601 gattttttat ttttatattt actggcaata ttgcctaaat aattaagtaa ggaatctgat
    12661 gggatcaggg atcgaaaatc aaaatcaaaa tagataggct gcacatactc taaatcagaa
    12721 ttggcggtga aggttattat gtcttcaatg gtaagtacat agggtctgta cagcattttg
    12781 tccagtacaa ttaaatcatt tgtttccttg accccagtac caatattgat catttctatt
    12841 cgatatccca ttgaatcaat caaatactgc cttagtactt cggctcttgt atagcctaaa
    12901 tcgcctaatt cagtttgatt gtactcctcc tttgagtaat tgccattgat cctcaatgct
    12961 ttgtccggat gatcctcaag ataatgtttc agattggtaa agttgcttct gaattgatcc
    13021 ctgcaataaa attccggctt ggcagatttc cacttacaat ggatgttccc tctcagctta
    13081 atggaatacc cgccagtctc atcaacgatc tccaaagggg ccggttcaag tctgtgagtg
    13141 aggcatcggt ccatatccac atcgattttt ttaataattt catgctccag tctttcctgc
    13201 atgaagataa aataataaat cgtgcatagt aaaacccacc ctgccaatag taccaataat
    13261 ttttgaatat agggaagctt caggtagtac attttacatg gattctttct tgtgaatcag
    13321 tatcatttcg gattcttcgt cagatagatt aatttggcgg ccgatttcat atacttctgc
    13381 ccttgtggta ttctcgtcaa tcatagtcat gacaagctcc ttttgtgctt ctttttccat
    13441 ttggcgtaac acttgtgaag aaagccccat ttgtgtagca tattcctgaa tgattcctgt
    13501 ttctgtttca tgaggttgta agtctgaata gcttagtata aaggcgcatt tgactatttg
    13561 cttcttggca ttttcagata aagcttttag ttccaatggt gatatgtaat ccatatttct
    13621 gattttttcg acacttccca attctttagg aagaatcttg ttaagaaatt cagcttcttc
    13681 acctgaaaga ttaccgtcag catcgcatat ctcgaccaaa actctggcaa gaacttcctt
    13741 ttcaagtctg tttaatccgg gttctttgtg tggctccggg cgattcttca cttctttggt
    13801 cgggctgttg ggctttggag tgggttttcc ggatctactg tttggagaat cagttggagt
    13861 ccaattgccg gaattttcat catatctata cctggttgaa attttctcaa aggcttttac
    13921 tacagcgtca tttatatcat cctggcttgg ttcaaaagaa aaagaactgt tggccggaac
    13981 cgaagctctg ataatcgtgg atcctgccct gcctaaaatt ccacctccta aaagactgaa
    14041 aatagattgt gatgcagtag ctctagccct gttaatcatt tgattcttta cgacacgcgt
    14101 gacgcgattc atcgtagagt tgttgtgagg aattgaagca ttactgctga tcagttcacc
    14161 attcaggtgg taaaatgaac attgcacttg atgtcctgtt gttgtcacat cctgtatcaa
    14221 gtgttcgatg gatgaataag ttatttcagg cattttaata atcgttaatt ttgtaaataa
    14281 atgtgaatag aatcttatgg accatgtttt gataatcgaa agataccttg atactctcgg
    14341 cgggcgacta ttgattggta aaatgcgaag tcattatctc cagtgtacgc tcagcactga
    14401 aagtacccaa ca
//
LOCUS       DAOQMC010000031        14337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1546_length_14337_cov_4.657961, whole genome shotgun sequence.
ACCESSION   DAOQMC010000031 DAOQMC010000000
VERSION     DAOQMC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1546_length_14337_cov_4.657961"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKV31_02305"
     CDS             complement(<1..788)
                     /locus_tag="PKV31_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNB91714.1"
                     /translation="MFSSLKSLGALNRFLVRYKYYLIGGFIFIALSNYFRVLQPRYFR
                     EALDMIIEVVPMYDVVKGSKHAALLYEYIIENLIWFGIFVILQAVIMGIFMYFMRQTI
                     IVMSRLIEYDMRKELYAHAQYLDTTFYKQHAIGDLMARFTEDVSRVRQYLGPVLLYGF
                     NLITLFVLVITAMFKVNTQLSIFTLLPLPLLSWSIFKISEKINVQSGYIQTQLSKLST
                     LSLEILSGIRDVKGYAREKAMQEMFAEETNVYVDKNLQRAKVDSL"
     gene            complement(914..1342)
                     /locus_tag="PKV31_02310"
     CDS             complement(914..1342)
                     /locus_tag="PKV31_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNB91715.1"
                     /translation="MDRREFIEKAGLACLAGIAGTSIITATGCKAATPTLTNSTHEGR
                     RVKILQADWPANTDFIIVRHPSDEHPIYIHKDGDNYIALHMKCTHRGCNLNAESNQLT
                     CPCHGSKFHADGKVKKGPAKKPLVGYKTTADTETIYVEIM"
     gene            1496..3052
                     /locus_tag="PKV31_02315"
     CDS             1496..3052
                     /locus_tag="PKV31_02315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNB91716.1"
                     /translation="MAQQDDFKRIVSFAKEYGFIYPSSEIYDGLSAVYDYGPLGVELK
                     NNIKQYWWQAMTRLHENIVGIDSGIFMHPLIWKASGHVDAFNDPMLDNKDSKKRFRAD
                     VLIENYIDKVADKINKEVEKAAKRFGDSFDADMFRSTNPRVLEYQKEIDTVSRRLADA
                     MTAGDMAELGKIIADLEIADPDSGSRNWTEVRQFNLMFSTQLGNISGQEDKLYLRPET
                     AQGIFVNFLNVQKTTRQKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFIAPG
                     TELEWFEKWKEMRMKWHLALGTDASKLRFKDHDNLAHYANAATDIQFEFPFGFKELEG
                     IHSRTDFDLKSHQELSGKKLTYFDQDRNESYIPYVVETSIGADRMFLAVLSNNLVEEN
                     VPDAKGEDSSRTVFKVHPALAPVKCAVLPLVKNKEELTALARKIHDNLHWQFQCQYDD
                     KDAIGRRYRRQDAIGTPICITVDFQSLEDGTVTIRDRDTLHQERVSIDALSAIIAGKT
                     DLSRVLRSLI"
     gene            3052..3978
                     /locus_tag="PKV31_02320"
     CDS             3052..3978
                     /locus_tag="PKV31_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNB91717.1"
                     /translation="MKEGRQQLSEELDKVEVLSTSSVMVRLMHAPLRYLCGQFFNKVI
                     YPITKKEKVVRAFTFFGVEMKVALPAGSDLLFTHGKSHDSELRLVRFLLREVKEGSQF
                     LDIGAHFGYFTLLAARLTGPTGKVCSYEPGKHTYAVLSQNVSGNAVINTFNRAVSSEN
                     TKLEFYEFPAYYSEYNTMDVSQFEQESWFARYKPEKIIVDAETIDSITSDGSIIPQFI
                     KIDVEGAEDRVIAGGRNYFKDNNPIVIMEYVSITRNNGNHKKAIEYLLSFGFSMAIIR
                     KDGSIDDMDPKKLDEYLESHGLESDNIVFRKV"
     gene            4395..4769
                     /gene="rpsL"
                     /locus_tag="PKV31_02325"
     CDS             4395..4769
                     /gene="rpsL"
                     /locus_tag="PKV31_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNB91718.1"
                     /translation="MPTINQLVRKGRKAVEYTSKSRALESSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNQIEVIAYIPGEGHNLQEHSIVLIAGGRVKDLPGVRYTIVRGAL
                     DTAGVNNRKKSRSHYGTKRPKK"
     gene            4802..5269
                     /gene="rpsG"
                     /locus_tag="PKV31_02330"
     CDS             4802..5269
                     /gene="rpsG"
                     /locus_tag="PKV31_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNB91719.1"
                     /translation="MRKRKPKVRLIEGDPRYNDTMVTKFVNHLMLEGKKSTAFQIFYD
                     AMDIVKEKLGEDEHEIWKKALNNAMPQVEVRSRRIGGATFQIPTELPANRKISIGMKW
                     LINFARKRSGKGMADKLSAEIIAASKGEGAAVKRKEDTHRMAEANRAFAHFRA"
     gene            5302..7434
                     /gene="fusA"
                     /locus_tag="PKV31_02335"
     CDS             5302..7434
                     /gene="fusA"
                     /locus_tag="PKV31_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB91720.1"
                     /translation="MSSNLDLTRNIGIAAHIDAGKTTTSERILFYTGISHKIGEVHDG
                     AATMDWMEQEQERGITITSAATRTHWDWQGKNYTINLIDTPGHVDFTVEVERSLRILD
                     GVVALFSAVDGVEPQSETVWRQADRYKVPRLGFVNKMDRQGADFFNVVKQVKEMLGGN
                     AVALQVPIGAEEHFKGVVDLITNEAIVWNESDYGMTFEVVPIPADLVDIVAEQREHML
                     ESVAEYDDTIMEKFFDDPDSISPDEIRAAIRKAVIDMKIVPMMCGSSFKNKGVQAMLN
                     AVCAFLPAPTDVEAVEGTNPKTDEPTFRKPSVTEPFAALAFKIATDPFVGRLAFMRVY
                     SGHLDAGSYVLNTRSDNKERISRLYQMHANKQNPIEKVDAGDIAAAVGFKDIKTGDTL
                     CDEKHPIVLESMTFPEPVIGLAIEPKTQKDLEKLGMALAKLAEEDPTFKVQYNEETAQ
                     TVISGMGELHLEIIVDRLRREFKVEVNQGAPQVNYKEALTKTVNHRERLKKQTGGAGL
                     FADIEFDLGPADESFLESEDFKNGKTKLQFVWDIVGGAIDKNYMKPITDGFTQMMSNG
                     VLAGYNIDSMKVRVHDGSMHPVDSKPIAFELAAKEGFKEAAKKAAPILLEPIMKLEVT
                     TPDEYTGSVIGDLNRRRGLVKGQDVKFNAVVVKAEVPLSEMFGYVTQLRTITSGRASS
                     SMEFSHFAQAPANIASDVIEKAKGAVKA"
     gene            7582..8130
                     /locus_tag="PKV31_02340"
     CDS             7582..8130
                     /locus_tag="PKV31_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNB91721.1"
                     /translation="MNLYFIALVAPEGISRKVLEWKHWMLDNFGCRVALKSPAHITLI
                     PPFRMASEQETHLRMYLDQFADRQKGFDIHLKDFSWFEPRVIFVQVMDNEALCELQHR
                     LQTCLVRTKEIPIHKVDHRFTPHVTIANRDLQKHDFYTAKKKFSEIQYSADWPVDRLS
                     LLPHNGLTWDIVHNAIFKEEAI"
     gene            8571..8879
                     /gene="rpsJ"
                     /locus_tag="PKV31_02345"
     CDS             8571..8879
                     /gene="rpsJ"
                     /locus_tag="PKV31_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNB91722.1"
                     /translation="MNQKIRIKLRSYDHNLVDVSTEKIVKTVRSSGAVVTGPIPLPTE
                     KKMFTVLRSPHVNKKSREQFSMRTHKRLIEIYTPTQKTVDALSRLELPSGVDIQVKLT
                     "
     gene            8960..9589
                     /gene="rplC"
                     /locus_tag="PKV31_02350"
     CDS             8960..9589
                     /gene="rplC"
                     /locus_tag="PKV31_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNB91723.1"
                     /translation="MNGIIGKKLGMTSVYDSKGNYVACTVIEAGPCVVTQVKNVETDG
                     YKALQIAFGDVKAKNVSKQLTGHFAKAGAEPMKTVIELRDSTLSKSLGETVEITDVLS
                     EGDKVDVLGLTKGKGFQGVVKRHGFGGVGQATHGQHNRLRAPGSVGASSYPSKVFKGM
                     RMGGQTGNTNVKIKNLKVLKIFSEQNLVLVKGAIPGHKGSVVIIESVKK"
     gene            9586..10209
                     /gene="rplD"
                     /locus_tag="PKV31_02355"
     CDS             9586..10209
                     /gene="rplD"
                     /locus_tag="PKV31_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNB91724.1"
                     /translation="MKLDILNKTGASTGRSYEFSSALLEEKPNEHAVYLAVKAYLANQ
                     RQGTHKAKERSEVAGSTRKLHKQKGTGGSRKGSIKSGVLHGGARIHGPRPRDYSQDLN
                     KKVKKVARRSVLIDKLLNNKVIIVEDFSFDTPKTKELHNIFTSLKADKKPLLVTGELN
                     KNVYLSGRNLPKTDVTFADELNVYDMLKADAVILFEGAVDKIVQSIA"
     gene            10231..10530
                     /gene="rplW"
                     /locus_tag="PKV31_02360"
     CDS             10231..10530
                     /gene="rplW"
                     /locus_tag="PKV31_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNB91725.1"
                     /translation="MIHEILIKPVITEKANDQSNQGKYTFVVAKKANKIEIGKAIEKM
                     YNVNVTSVNTHVMPVKVVTRSGRTGVMKGVKAAYKKAVVTLAPGESIDIFQNQEN"
     gene            10568..11395
                     /gene="rplB"
                     /locus_tag="PKV31_02365"
     CDS             10568..11395
                     /gene="rplB"
                     /locus_tag="PKV31_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNB91726.1"
                     /translation="MPVKKYNPMTPGTRYRVGNAFAEITTDTPEKSLIADLKKTGGRN
                     HDGKMTLRQRGGGHKRRYRIIDFKRNKDGIPAKVASIEYDPNRTAFIALLNYVDGEKR
                     YIIAPKGLKVGDTVISGTGVAIEPGNALMLKDIPLGAEVHAIELQPGKGAAIARSAGS
                     YALVVNKEERFAVIKLPSGEVRRILLTCKATVGIASNQDHSLLVFGKAGRKAWIGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPISRTGIMAKGQKTRNPNKKSSALIISRRKSK"
     gene            11422..11688
                     /gene="rpsS"
                     /locus_tag="PKV31_02370"
     CDS             11422..11688
                     /gene="rpsS"
                     /locus_tag="PKV31_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNB91727.1"
                     /translation="MARSLKKGPYVHYSLLEKIRKAKESSKKSVIKTWSRASMIIPDM
                     VGETIAVHNGKTFVPVYVTENMVGHKLGEFSPTRNFRGHGGKKK"
     gene            11729..12088
                     /gene="rplV"
                     /locus_tag="PKV31_02375"
     CDS             11729..12088
                     /gene="rplV"
                     /locus_tag="PKV31_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNB91728.1"
                     /translation="MEAQAKYRNIKGSERKMRLVVDTIRGKNVGQALAILKHTQRSSA
                     HSVYKTLLSAVANWEQMTGIESGADSHGLYVKTAFVDGAGMLKRFQPAPHGRAHRIRK
                     RSCHLTIVVDSKVPVEN"
     gene            12119..12898
                     /gene="rpsC"
                     /locus_tag="PKV31_02380"
     CDS             12119..12898
                     /gene="rpsC"
                     /locus_tag="PKV31_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNB91729.1"
                     /translation="MGQKTSPVGNRLGIIRGWDSNWFGGKDMAAKVVEDEQIRNYMSA
                     RIPKGGIAKIVIERQIKRITVTIHTSRPGIIIGKGGQEVDRIREELKKLTGKDVQINI
                     HEIRKPELDAAIVGETIAKQIESRINYKRATKMAIQSTMRAGAEGIKVRLSGRLNGAE
                     MARSEEFKEGRTPLHTFRVDIDYCISVAETVYGLIGIKVWICKGEVLGRRDIMIHQVE
                     DKKAGGHGGGRGDRGGDRGGDRGGNRGDKRGGSRNFKSKRQ"
     gene            12988..13410
                     /gene="rplP"
                     /locus_tag="PKV31_02385"
     CDS             12988..13410
                     /gene="rplP"
                     /locus_tag="PKV31_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNB91730.1"
                     /translation="MLQPKRTKYRKQHKMNPKGVAHKGSTISFGSIALKAVTGGRLTA
                     RQIESARIAMTRYMKREGKVWIRIFPDKPITKKPLEVRMGKGKGPVDHYVAVVKPGRI
                     LFEMDGVSPEVATEALRLAAQKLPVLTKTILRTDIRIA"
     gene            13432..13686
                     /gene="rpmC"
                     /locus_tag="PKV31_02390"
     CDS             13432..13686
                     /gene="rpmC"
                     /locus_tag="PKV31_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNB91731.1"
                     /translation="MANKQYKEIQNLADNELVEKLAQTQLDLTKARFDQTITGNVSQK
                     DIRESRKVIAHIQTEIRSRELAQMTESQLAKRSRIRNRRK"
     gene            13725..13988
                     /gene="rpsQ"
                     /locus_tag="PKV31_02395"
     CDS             13725..13988
                     /gene="rpsQ"
                     /locus_tag="PKV31_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNB91732.1"
                     /translation="MEQIVRKARKERIGKVTSNKMDKSISVMVERRIKHPLYGKFVKK
                     SNSFMAHDEKNECQIGDIVKIMETRPLSKNKRWRLVEIIEKAK"
     gene            14018..>14337
                     /gene="rplN"
                     /locus_tag="PKV31_02400"
     CDS             14018..>14337
                     /gene="rplN"
                     /locus_tag="PKV31_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNB91733.1"
                     /translation="MIQTESRLNVADNSGAKEVLCIHVLGGSTRRYASIGDKIVVSVK
                     QASPGGIKKGTVSKAVIVRTKKEIRRADGSYIRFDDNAVVLLNNQDEPRGTRIFGPVA
                     RELR"
BASE COUNT         4399 a         2718 c         3374 g         3846 t
ORIGIN      
        1 agagaatcta ccttggctct ttgtaagttt ttgtcaacat aaacattggt ttcttcggca
       61 aacatttctt gcatcgcttt ttcccgggca tatcccttga catctcttat gccacttagg
      121 atttcgagcg ataaagtact caactttgac agttgggtct gtatataacc actttgcaca
      181 ttgattttct cacttatttt aaagatgctc cagctaagca agggcaaagg aagcaaggta
      241 aaaatagata attgagtatt gaccttaaac atcgccgtaa tgactaatac aaataaggtt
      301 atcaggttga agccataaag caataccggt cccagatatt gacggacacg acttacatcc
      361 tcggtaaatc gtgccattag atcaccaata gcatgctgtt tataaaaagt tgtgtctaaa
      421 tattgcgcat gagcatacaa ttccttgcgc atatcatatt caataagcct ggacatgaca
      481 atgattgtct gacgcatgaa atacatgaaa atccccatga tgacagcctg taggattacg
      541 aatatgccaa accagatcag attttctata atatactcat agagcaatgc ggcgtgcttg
      601 ctgcccttga caacatcgta catgggcacc acctcaatga tcatatccaa cgcctcccga
      661 aaatacctgg gttgtaatac cctgaaataa ttggacaagg caataaaaat aaatccaccg
      721 atcaggtaat acttgtaccg gacaaggaat ctattgagag cgccaagact tttaagggaa
      781 gaaaacatcg accaaagata atatgattca agggaatgta gcgtttttgt cttatgattg
      841 atgggacaaa acgtcattcc agggtagaag atagcttgtt ttcttactcc tcccgggagg
      901 atgtgctgac agtctacatt atttctacat agatggtctc tgtatcagcc gtggttttgt
      961 agccgaccaa tggttttttg gcgggacctt ttttgacctt tccatcagca tggaatttgc
     1021 ttccgtgaca aggacaggtc aattgattgg attcagcatt gagattgcat ccgcggtgag
     1081 tacatttcat atgcagtgcg atgtagttat ctccatcttt gtggatatag atgggatgct
     1141 catcgctcgg atggcgtact atgataaaat cggtatttgc cggccagtca gcctgtaaga
     1201 tttttaccct tctgccttca tgtgtgctgt tggtcaatgt aggagtagca gctttacagc
     1261 ctgtggcagt gataatactc gtcccggcta tccctgcgag acaagccaga cctgcttttt
     1321 caatgaattc tcttctatcc atgttcctat agttttacgt ggactaaata taggaaaatt
     1381 ttttcaaatg aaggttcaga ctgatgttat accaagatta attggctttc ttcattgtgg
     1441 aaaataatat ttacctttgc ggcctgaaac caaataatat taaatttctt cagatatggc
     1501 gcaacaggat gatttcaaga gaatcgtttc atttgctaag gaatacgggt ttatttaccc
     1561 ttcaagtgag atttatgatg gactaagtgc agtgtatgat tacggtcctt taggtgtgga
     1621 attgaaaaac aacatcaagc aatactggtg gcaggccatg acccgattgc atgagaatat
     1681 tgttggtatc gacagtggta tcttcatgca tccactgatc tggaaggcaa gtggccatgt
     1741 tgacgccttc aatgatccga tgctggacaa caaggatagt aaaaagcggt tccgcgctga
     1801 tgtgctgatt gaaaattata tcgacaaggt ggctgacaag atcaacaaag aggttgagaa
     1861 agcagcaaag cgatttggag actcgtttga cgccgatatg ttcaggtcaa ccaatccacg
     1921 ggtccttgaa tatcagaaag aaatagatac agtatccagg cgcctggccg atgccatgac
     1981 tgccggcgat atggctgaat tgggcaagat tatagccgac cttgaaatcg ctgatccgga
     2041 ctccggttcc cgcaattgga ctgaggtgcg tcagtttaac ctgatgttct ccacccagct
     2101 gggaaatatt tccggtcagg aagacaagtt gtatctgcgt cctgagactg ctcagggaat
     2161 cttcgtcaac tttctcaatg tgcagaaaac aaccagacag aagattcctt ttggtattgc
     2221 tcagataggc aaggccttca ggaatgagat tgtagcgcgt cagtttatct tccgtatgcg
     2281 cgaattcgaa caaatggaaa tgcaattttt cattgctcct ggtaccgaac tcgagtggtt
     2341 tgaaaaatgg aaggaaatga ggatgaaatg gcatcttgcc ttaggtaccg atgcatctaa
     2401 actccgcttt aaggatcatg acaacctggc gcattatgcc aatgccgcga cagatataca
     2461 atttgaattc ccattcggat tcaaagagtt agaaggtatt cattcaagaa cagacttcga
     2521 cctgaagagc catcaggaat tgtccggaaa gaagcttacg tatttcgatc aggatcggaa
     2581 cgaatcttat atcccgtatg tcgtagagac atctatcggt gctgacagga tgttccttgc
     2641 agtgctgagt aacaatctgg ttgaggaaaa tgtaccggat gccaaaggtg aggattcaag
     2701 cagaactgta ttcaaggtac atccggctct tgcacctgtc aaatgtgccg tgttgccttt
     2761 ggtgaagaac aaggaagagc tgaccgccct ggcgcgtaaa attcacgaca atctgcactg
     2821 gcaattccag tgtcaatacg atgacaaaga tgcaataggt cgccgatacc gaaggcagga
     2881 tgcgattggg actccgatat gtattacggt ggacttccag tctttggagg atggtacagt
     2941 gacgatacgt gatcgggata ccctacatca ggagagggtg tccattgacg cactaagtgc
     3001 aattatcgcc gggaaaacgg atctttccag agttctccgt agtttgattt gatgaaagaa
     3061 ggcaggcaac aattgtctga agagttggac aaggttgaag tcttgtcgac ttcctctgtt
     3121 atggtccggt tgatgcatgc tccattgcgg tatttgtgtg gccagttttt caataaagtg
     3181 atttatccga ttaccaaaaa ggagaaagtt gttcgcgctt tcactttttt tggtgtggaa
     3241 atgaaggtgg cgttgccggc aggatcggat ttgttattta cccatggaaa gtcacatgat
     3301 tctgagttgc ggctggttcg attcctgctc cgtgaagtga aagaagggag tcagtttctg
     3361 gacataggtg cacatttcgg ctattttacc ttattggcag caaggctaac aggaccgacc
     3421 ggtaaggtct gttcatacga gccaggtaaa catacctatg ctgtgctttc gcaaaatgtt
     3481 tccggtaatg ctgtcattaa taccttcaat cgtgcagtgt cttcggagaa taccaagttg
     3541 gaattctacg aatttcccgc ttattattcc gaatacaaca ccatggatgt cagtcagttt
     3601 gagcaagaat catggtttgc ccggtacaag ccggaaaaga taatagttga cgcagaaact
     3661 attgactcga taacatcgga tggttcaatt attccgcaat ttatcaagat tgatgtggaa
     3721 ggggcggaag atcgggtgat cgccgggggc cgtaattatt ttaaggataa taacccaatt
     3781 gtgattatgg aatatgtatc catcacccgt aataatggca atcataaaaa agcaatcgaa
     3841 tacctcttgt cttttgggtt ttcaatggcg attatccgga aagatggatc gatagatgat
     3901 atggatccaa agaaattgga tgaatatctt gaaagtcatg gtcttgagtc cgataatatt
     3961 gtattcagga aggtttgatg gattgggtgg attttgatgt aaatgcatga ttccggaggt
     4021 cactcattgg atgtgccggg aactcattcc ttagttgatg ctatttctgc caaaaccttg
     4081 cataagggaa ggtgtttccg gaacagagaa attgtagatc tgctcaaatt aatgagacaa
     4141 aatttaagaa atcaatattg gaataaatga aatgatcatt gctaattggt taaacatact
     4201 aagttaaatt agaattagcc cgtaagattt tctaaaatat ttttactcaa acacttgtat
     4261 agaactaaaa tagtcataac tttgcaacct gttttgaaaa aggggaggaa gtaactgttg
     4321 aaaacatagt agacaagcga cttttgaggc tttttcaagg aatttttaac taatcatttt
     4381 ttaaacaaaa tcgtatgcct actattaatc aattggtaag aaaaggcaga aaagcagtgg
     4441 aatataccag caaatcaaga gcgctggagt caagccctca gaaaagaggt gtttgtacca
     4501 gggtatatac tactacacct aaaaagccaa actctgcact tagaaaagtt gccaaagtca
     4561 gacttaccaa ccagatcgaa gtcatcgctt acatcccggg tgaaggccac aacttgcagg
     4621 aacactccat cgtgttaatt gcaggtggta gggttaaaga ccttccgggt gtacgttaca
     4681 ctatcgtaag aggagcactg gatacagccg gtgtaaacaa caggaagaaa agccgttcac
     4741 attacggcac aaagcgcccg aagaaataat ttattaatta aataagcaac aagctttaaa
     4801 aatgagaaaa aggaaaccaa aagttagatt gattgaaggc gacccaaggt acaatgatac
     4861 catggtgact aagtttgtca atcatttaat gcttgaaggg aagaagagta ccgcctttca
     4921 aattttttac gatgcaatgg atatcgtaaa ggaaaagctc ggagaagacg agcatgaaat
     4981 ctggaaaaaa gcattgaaca atgctatgcc ccaggtcgaa gtacgttcac gcagaatcgg
     5041 aggtgcgacc ttccagattc ctactgaact tccagccaac agaaagatct ccattggaat
     5101 gaaatggttg atcaacttcg cacgcaagcg aagcggcaaa gggatggccg acaagttgtc
     5161 tgctgagata attgcagcaa gcaaaggtga aggcgccgca gtgaagcgta aggaagatac
     5221 acacagaatg gctgaggcta acagagcgtt cgctcatttt agagcttaat atttaatcag
     5281 gacttttaaa ctaaataata aatgtcttct aatttagatt taacaaggaa catcggaatt
     5341 gctgcccaca tcgacgcggg taagacaacc acttccgagc gtattctttt ctatacaggt
     5401 atcagccaca aaataggcga ggtacacgat ggtgctgcga caatggactg gatggagcaa
     5461 gagcaggaaa gaggtattac cattacgtca gctgctacaa ggactcactg ggactggcag
     5521 ggaaagaact ataccatcaa cctgattgat actcccggac acgttgactt cacagttgaa
     5581 gttgagcgtt cccttcgtat cctcgatggt gtggtagcac ttttcagtgc agtggatggt
     5641 gtggagcctc agtcagagac tgtttggcgt caggctgatc gctacaaagt tccacgtttg
     5701 ggctttgtca ataagatgga ccgtcaggga gcagatttct ttaatgtagt gaagcaagtt
     5761 aaggaaatgc tcggtggcaa cgctgtggca cttcaggtac ctattggtgc ggaagaacac
     5821 ttcaaaggtg ttgtggattt gattaccaat gaagctatcg tatggaatga aagcgattat
     5881 ggaatgacct ttgaggtcgt accgattcct gctgatcttg tcgatatcgt tgcagaacaa
     5941 cgcgaacaca tgctggagtc tgttgcagaa tacgatgata ccatcatgga aaaattcttt
     6001 gatgatccgg attccatttc tcctgacgag atcagagctg ctatccgtaa agcggttatc
     6061 gatatgaaga ttgttccaat gatgtgtggc tcttccttta aaaataaggg agttcaggca
     6121 atgcttaatg ctgtatgtgc cttcttgcct gcaccaaccg acgttgaagc agtagaaggt
     6181 accaatccta agacggacga accaactttc agaaagcctt cagttaccga gccgtttgct
     6241 gctttggcat tcaaaattgc tactgacccg tttgttggcc gattagcttt catgcgcgta
     6301 tattccggtc atttggatgc aggttcatat gtattgaata ccagatccga taataaggag
     6361 cgtatttcac gtctttatca aatgcacgcc aacaagcaga atccgatcga aaaggtggat
     6421 gcaggtgata ttgccgctgc tgttggtttt aaagatatca agaccggaga tacactgtgt
     6481 gatgaaaagc atcctattgt tctggaatca atgactttcc ctgagccggt tattggtctt
     6541 gctattgagc ctaagacgca gaaagatctt gagaaattgg gtatggcact tgcaaaatta
     6601 gctgaagaag acccaacctt taaggttcaa tataatgaag agactgctca gacagtaatt
     6661 tccggtatgg gtgaattaca cctggaaatc atcgttgatc gtcttcgccg tgaatttaag
     6721 gtggaagtca atcaaggtgc tccacaggtt aactataagg aagcgcttac caagaccgtc
     6781 aatcaccgtg agcgccttaa gaagcagacc ggtggtgctg gtctcttcgc tgatatcgag
     6841 tttgatctgg ggccggctga tgaatcattc ctggagagcg aagatttcaa gaatggcaag
     6901 accaagcttc agtttgtttg ggatattgtt ggtggtgcca tcgacaagaa ctatatgaag
     6961 cccattaccg atggattcac gcaaatgatg agtaatggtg ttttggcagg ttataacatt
     7021 gatagcatga aagttagggt acacgatggc tctatgcacc ctgtggactc caagcctatt
     7081 gcattcgaat tggcagctaa ggaaggattt aaggaagctg caaaaaaagc tgccccaatc
     7141 ctgcttgaac ctatcatgaa gttggaggtg actacaccag atgaatatac cggttctgta
     7201 attggtgacc ttaaccgacg tcgtggtctg gtaaaaggcc aggacgtcaa gttcaacgcc
     7261 gtagtcgtta aggctgaggt tccattgagt gagatgtttg gttatgttac tcagctgcgt
     7321 accatcacct caggacgtgc atcttcatcc atggaattct cacacttcgc tcaggctcct
     7381 gccaatatcg cttcagacgt gatagaaaag gcgaaaggtg ctgtaaaagc gtaattgtaa
     7441 ggaagaaatt ccttgtcaag atagaagcct cgttcaaccg aacggggctt ttttttgata
     7501 tttttccata agtaacactg gtttttgtaa ttgttatttc cgggtattta ttcttttgaa
     7561 ataatttcat agcttagatg catgaacctg tattttattg cattggttgc gcccgaagga
     7621 atcagccgaa aggttctcga atggaagcat tggatgctgg ataactttgg ctgtcgcgta
     7681 gccctgaaat ctccggctca cattacgctg attccccctt tcaggatggc atctgagcag
     7741 gagactcatc tgaggatgta cctggatcag tttgctgacc ggcaaaaagg attcgacata
     7801 catttgaaag acttttcctg gttcgaacct cgggtaatct ttgtgcaggt gatggacaat
     7861 gaagcgttgt gtgagttgca acatcgtttg cagacctgcc ttgttcggac gaaggagatt
     7921 cccattcata aggtggatca tcgatttact ccccatgtga cgatagccaa tcgggatctt
     7981 cagaaacatg atttttacac agcaaagaaa aaattttcgg aaatacaata tagtgctgac
     8041 tggccggtag atcgattgtc cttactacca cacaatggcc tgacatggga tattgtacat
     8101 aatgcaatat tcaaagaaga agccatttga atactgatga ccatgggatg tatttgttcg
     8161 gcttacggaa acattggttt tcagttgacc tgacaagatg accggtcaac agataagtct
     8221 acatttttct gatttgagta ttggtaattt ttggaggcaa gaatgcaagt tttattagaa
     8281 atgggattct gtattttatt gtgatagtta tgggtagtaa tgctaaaaat ttgtagaaat
     8341 tggattcctt ggtattttta agttaataaa cccttgaagt gaagatatct tattttccgg
     8401 ttcatttcat ttgccgatga tttgaagagg accatttgca tggattgtcc cttttttcaa
     8461 aatattttta caaaaaaatc ataaaaaata ttgccacaat caaaatgttt attacctttg
     8521 cgccacttta aaaagaaaac catcttcatc tgtattaatt aattgaggat atgaatcaga
     8581 aaattagaat taaacttcgt tcttatgacc acaacttggt ggacgtatct acggagaaaa
     8641 ttgttaagac agttcgctca agcggagctg tcgtaacggg tccgattccg ctgccaaccg
     8701 agaaaaaaat gtttaccgtg ttgcgctcgc cacacgtcaa caagaagtct cgagagcagt
     8761 tttcaatgcg tacccacaaa cgtttgattg agatttacac tccaacgcag aagacagttg
     8821 atgctctatc gaggctggaa cttccaagtg gggtagatat ccaggtcaaa ctgacttaag
     8881 gaataatttc atttttaata aataaattgg tggcattgag gacataactt cctctgtgtc
     8941 tttattaaat aatattgtcg tgaacggaat tatcggaaag aaattaggca tgaccagcgt
     9001 atatgactca aaaggtaatt atgttgcctg cacagtcatc gaagctggcc cttgtgtggt
     9061 tacacaggtg aagaatgtcg aaacagacgg ctacaaagct ttacagattg cattcggtga
     9121 tgtcaaggca aagaatgtgt ccaagcaatt gactggtcac tttgctaaag cgggtgctga
     9181 acctatgaag actgttattg agttgagaga ctctacactg tctaaatctc tcggtgaaac
     9241 agtcgaaata actgatgtcc ttagtgaagg cgataaggtt gatgtattgg gtctcaccaa
     9301 aggtaagggc tttcagggtg tcgtcaagcg tcacggtttc ggtggtgtcg gtcaggctac
     9361 tcacggtcag cataacagat tgagagcccc cggttcagtc ggtgcatcct cttatccttc
     9421 caaagtattc aagggtatgc gtatgggagg ccagactgga aataccaacg taaaaatcaa
     9481 aaacctgaag gtacttaaaa tattttctga gcagaacctt gttcttgtca aaggagctat
     9541 ccctggacat aagggctctg tcgtaatcat tgaaagtgtt aagaaatgaa actggatatt
     9601 ctaaataaaa caggggcgtc aaccggacgt tcatatgaat tctcttcagc attgctcgaa
     9661 gagaagccaa acgagcatgc ggtgtatctt gcagttaagg catatcttgc caatcagcgc
     9721 cagggcacac acaaagccaa ggaaagaagt gaagtggctg gttcgacacg taagttacat
     9781 aaacaaaaag gcaccggtgg ctctcgtaaa ggtagtataa agtctggcgt tctgcatggt
     9841 ggtgccagaa tccatggacc acgccctcgg gattatagcc aggatctcaa caaaaaagtt
     9901 aagaaggtgg cacgaagatc tgttctgatc gataagctgc tcaataataa ggtaatcatc
     9961 gttgaggact ttagttttga tactccgaag accaaggaat tacataatat tttcacctca
    10021 ttgaaggctg acaagaaacc tttactcgtc acaggtgaac ttaacaagaa tgtttatttg
    10081 tcaggacgaa acctaccgaa gacggatgtg acttttgctg acgagctcaa tgtatatgat
    10141 atgttgaaag ctgacgcggt gatcctgttc gaaggtgcag ttgacaagat tgttcaatcc
    10201 attgcttaat acttaatcat tagaaaagcc atgatccacg aaatactcat caagccggtc
    10261 attactgaaa aagcaaatga ccagtcaaat cagggaaaat ataccttcgt agttgcgaag
    10321 aaggcaaaca agattgagat tggtaaagcg atcgagaaga tgtacaatgt caacgttaca
    10381 tcagtcaaca cacacgtaat gcctgtaaaa gttgttacga gaagtggtcg cacaggagtc
    10441 atgaaaggtg ttaaggcagc ttataaaaag gccgtcgtta cccttgctcc aggtgaatca
    10501 attgacattt tccagaatca agaaaattaa tcaccaccta ttaagaaatt ttaaactgta
    10561 agctgttatg ccggttaaga aatataatcc gatgactccg ggaacccgtt atagagtggg
    10621 aaatgctttt gctgaaatca cgacggatac tcccgaaaaa tccctgatcg ctgatttgaa
    10681 aaaaacaggt ggtcgtaacc atgatggtaa gatgacctta cgtcagcgag gaggtggtca
    10741 taagcggaga taccgtatca tcgactttaa gcgtaataaa gatggaatac ctgctaaagt
    10801 tgcttccatt gagtatgatc cgaacaggac tgcatttatc gcacttttga attatgtcga
    10861 tggtgaaaaa cgttacatta ttgctccaaa aggtttgaaa gtcggtgata ccgtgataag
    10921 cggtaccggc gttgctatcg aaccgggtaa tgcactgatg ttgaaggata ttccattagg
    10981 tgctgaagta catgcaatcg agttgcaacc cggtaaaggt gcagctattg ccagaagtgc
    11041 cggctcatac gctttggtgg taaacaaaga agagcgattt gctgtcatca aattgccttc
    11101 cggcgaggtc agacgtatcc ttcttacttg taaggctaca gttggaattg catcaaatca
    11161 ggatcacagt ttgctcgtat tcggtaaggc tggtcgtaag gcttggatcg gaagacgacc
    11221 aagagtaagg ggtgttgcaa tgaacccggt tgaccacccg atgggaggtg gtgaaggtcg
    11281 cgcttcagga ggtcatccga tatcgcgaac cggaatcatg gctaaaggtc agaaaacaag
    11341 aaatccaaat aagaaaagct ctgctttgat tatttcaaga cgaaaatcta aataataacc
    11401 gaaatctgaa atattagcat tatggctaga tcattaaaga aaggtcctta cgttcactat
    11461 tcactcctcg aaaaaatcag aaaagcgaag gagtcttcca aaaaatctgt gattaagacc
    11521 tggtccagag catcaatgat tatccccgat atggtgggcg aaaccattgc tgttcataat
    11581 ggtaagactt ttgttcctgt ctatgtaact gaaaacatgg taggacataa actgggagaa
    11641 ttttctccga cacgtaactt cagaggtcac ggaggtaaga agaaataatt tttaataata
    11701 gaaagttggc ttttctaaat aaaatataat ggaagcacaa gctaaatatc gaaatatcaa
    11761 aggatcagag cgtaagatgc gtctggttgt tgataccatc agaggcaaaa acgttggcca
    11821 ggctctcgca attttgaagc atacccaacg ctcttcagca cattcagtat acaagacctt
    11881 attgtcagca gttgccaatt gggagcaaat gaccggaatc gaatcgggtg ctgacagcca
    11941 tggactatat gtcaagactg cattcgttga cggtgccggc atgttgaaga gattccagcc
    12001 tgcaccacac ggtagggctc acagaatccg aaagcgatca tgccatctga caattgttgt
    12061 cgatagcaaa gttccagttg aaaattaata ccataattaa atttaaataa ccttattcat
    12121 gggtcaaaag acaagtccgg taggtaatag attgggaatc atcagaggat gggattccaa
    12181 ttggttcggt ggaaaagata tggctgccaa ggtggtagag gatgagcaaa tcagaaatta
    12241 catgagtgct cgtataccga aaggtggcat tgctaaaata gtaatagaac ggcaaatcaa
    12301 aagaattacg gtaactattc atacttcccg cccgggtatc atcatcggta aaggaggaca
    12361 ggaagtcgac cgaatccgcg aagagttgaa gaaactaacc ggaaaggacg ttcagatcaa
    12421 tattcacgaa atccgtaagc ctgagctgga cgctgctatc gtcggtgaga ccattgcaaa
    12481 gcagattgaa tctcgtatca attataagcg tgcgacaaag atggctatcc aatccacaat
    12541 gcgtgcagga gcagagggaa tcaaggtcag attgtccgga agattgaatg gcgctgaaat
    12601 ggctcgttcg gaagagttca aagaaggcag gacaccattg catacattcc gggtggatat
    12661 cgattattgt atatctgttg cagaaacagt atatggattg attggtatta aagtgtggat
    12721 ctgtaagggt gaagtcctag gacgccgaga tatcatgatt catcaggtag aagataagaa
    12781 agctggtgga catggtggtg gccgtggaga ccgtggaggt gacagaggag gagatagagg
    12841 cggcaaccgg ggcgataaac gaggcggcag cagaaatttt aaatctaaaa gacagtaaat
    12901 aaagattttt gatgtatctt tgcacctcgt ttgaggaaag taaatacaac tatcttttaa
    12961 ccagcgaatt ataaagaaag acgtaccatg ttacaaccaa aaagaactaa atacagaaag
    13021 caacataaaa tgaatccgaa gggtgttgca cacaaaggta gtacgatatc tttcgggtcg
    13081 atagctctta aggcagtgac cggcggacgt ctgactgcac gtcagattga atcagccagg
    13141 attgctatga ctcgctatat gaaaagggaa ggaaaggtgt ggatcaggat tttccctgat
    13201 aagccgatca ccaaaaaacc tttggaagtt cgtatgggta agggtaaagg tcccgtggat
    13261 cattatgttg cagtggttaa gccgggtaga attctctttg aaatggacgg agtctctccg
    13321 gaagttgcta ctgaggcact cagattggca gcacagaagt tgcctgtttt gaccaaaact
    13381 atcttgagga cagatattcg tattgcttaa ttttgaaacc agcactatat catggcaaac
    13441 aaacaatata aagagatcca gaatctggca gacaacgaac tggttgagaa attggcccag
    13501 acacagttgg atttgaccaa agccagattt gatcagacta tcacaggcaa tgtttctcag
    13561 aaagatatca gagaatccag gaaagtgatt gctcatattc agactgaaat ccgcagtagg
    13621 gaattggctc agatgactga aagtcaactg gctaagaggt ccagaatcag aaatagaaga
    13681 aaataataac caccggttaa ataaatttta aagtatcctt tgatatggaa caaattgtaa
    13741 gaaaagccag aaaagagagg attggaaaag taacctccaa taaaatggac aaatccatct
    13801 ctgttatggt cgaaagaaga atcaaacacc cattatacgg taaattcgtt aagaaatcca
    13861 attcatttat ggctcatgat gagaaaaatg aatgccagat tggagatatc gtaaaaatta
    13921 tggaaacacg tcctctatct aagaataaga gatggagact ggtggaaatt atcgaaaaag
    13981 cgaagtaatt taataacagg aataatcttt atttgtcatg atacagacgg aatcaagact
    14041 aaatgtggcg gacaacagcg gggcgaagga agttctttgc atccacgtcc tcggcggctc
    14101 gacccgcaga tatgcttcta tcggagacaa gatcgttgtt tcggtcaagc aggcttctcc
    14161 gggaggcatt aaaaaaggta cagtttcaaa agctgttatc gtaagaacaa aaaaggaaat
    14221 tagacgtgct gacggttcgt atatccgttt tgatgataat gccgtagttc ttttaaacaa
    14281 tcaggatgag ccacgcggta cgcgtatatt tggtcctgtt gcaagagaat tgcggga
//
LOCUS       DAOQMC010000032        14170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1578_length_14170_cov_5.221183, whole genome shotgun sequence.
ACCESSION   DAOQMC010000032 DAOQMC010000000
VERSION     DAOQMC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1578_length_14170_cov_5.221183"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            579..2249
                     /gene="asnB"
                     /locus_tag="PKV31_02405"
     CDS             579..2249
                     /gene="asnB"
                     /locus_tag="PKV31_02405"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HNB91734.1"
                     /translation="MCGILGIFDLRQSPDSLRAQALAMSRKQRHRGPDWSGIFTCNNA
                     ILAHERLSIVDPTSGKQPLFSQDGAIVLAVNGEIYNHRQLKKGLTTPYPFQTQSDCEI
                     ILALYQEKGIDFIEDLNGIFAFALYDHANDSYLIARDHMGIIPLYMGWDEYGNFYVAS
                     EMKALSGICNKIREFLPGHYLYSRDGEMKKWYKRDWMEYDNVKDNETDIQKLRSAFEE
                     AVHRQLMSDVPYGVLLSGGLDSSIVSAIAKKYAARRIETEDLKDAWWPQLHSFAVGLK
                     DSPDLAASRKVAEHIGTIHHEIHFTIQEGLDAISDVIYHIETYDVTTIRASTPMYLLA
                     RVIKSMGIKMVLSGEGSDEIFGGYLYFHKAPDARAFHEETVRKVGKLHLYDCLRANKS
                     LAAWGVEGRVPFLDKEFMDVAMRINPKDKMAGNGKIEKYILRKAFEDYLPHEVAWRQK
                     EQFSDGVGYSWIDTLKLITAERVTDEQLNNAAHRFPLNTPTTKEEYYYRSLFAEHFPG
                     SEEAACVPFEASVACSTAIALEWDEAFKKMKDPSGRAVSSVHEAGYKE"
     gene            complement(2274..3263)
                     /locus_tag="PKV31_02410"
     CDS             complement(2274..3263)
                     /locus_tag="PKV31_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB91735.1"
                     /translation="MYLTINLYSQEVIRLTNPSFEDIPGHSRPPIGWRDCGAFLFPGE
                     SPPDVQPSGSWEVRKPANNGDTYLGMVVRENDTWESVSQQLSGTLKKGKTYTFDLFLC
                     TSEVYMSFTKANRSTESNYTTPAKLRIWGGNSLCSKEVLLAESSLITNNNWKKYYFTF
                     KPTKNVNYIVLEAFYKTPTLVPYNGNILVDNASNIVEVIKESPVTVKELTQKVTPGQI
                     IKLDQLYFKADNSSLDKNSHKVLNEVVDFMQRNEAVKIEIGGHTNGVPSVEYCQKLSS
                     ARAREVANYIINKGIDKNRVTYKGYGKSKPIASDRTPEGRKKNQRVEIKILST"
     gene            complement(3496..4707)
                     /locus_tag="PKV31_02415"
     CDS             complement(3496..4707)
                     /locus_tag="PKV31_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB91736.1"
                     /translation="MDHKPTTHHKSNKSDLFQSHDYYAIDELLTEDHILAREAVRAWV
                     KQEVSPIIEDYANRAECPTHLFKGLGEIGAFGPSIPAEYGGGGMDEIAYGIIMAELER
                     GDSGIRSMASVQGSLVMYPIFRFASEEQKRKYLPKLATGELIGCFGLTEPDHGSNPGG
                     METNIVDDGDAFILNGSKMWITNSPVADIAVVWAKDEEGVVRGMVVEKDTPGFSAPEI
                     HGKWSLRASITGELVFSDVRVPKANVFPDIRGLKGPLSCLSKARYGIAWGVLGAALDC
                     YDSALRYSRERIQFGKPLGQFQLTQKKLAEMITEITKAQLLAWRLGTLANQGKATPAQ
                     ISMAKRNNVHMALEVAREARQIHGGMGITNEYPIMRHMMNLETVLTYEGTHDIHLLIT
                     GMDVTGFNAFS"
     gene            complement(4931..5536)
                     /locus_tag="PKV31_02420"
     CDS             complement(4931..5536)
                     /locus_tag="PKV31_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB91737.1"
                     /translation="MKILDGIISKYEGHSMAHALAGRIAARQGNIAFAIKKFDLATEL
                     DPCLTEAYLWKGKMLIREERYKEALPVLEKVTECSIAHQNIHWIGRRLKGKCYFHLGD
                     FEKAIFEWKLFTKRPFEKGNSNHYWKRMVTFSLGKAYLHTNQHAEAILSFNNALELDP
                     LFESVSEEEIMKYKAIAGSGQEESGNSKKSTRKSTGVKATA"
     gene            complement(5533..5928)
                     /locus_tag="PKV31_02425"
     CDS             complement(5533..5928)
                     /locus_tag="PKV31_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91738.1"
                     /translation="MFSKVIVQGRLDFGNANTYDKALKLYTQRKDVYYKNEVIFNKME
                     ECFDEENLIFFVKRFNNLVSDKYWRNTVHLLKNLAEYSVTGRVEMWMMVDGKVIRHEQ
                     IRPSSDKSIVASFIRPMKLSMKTVTFPKL"
     gene            6258..7049
                     /locus_tag="PKV31_02430"
     CDS             6258..7049
                     /locus_tag="PKV31_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB91739.1"
                     /translation="MKNLFLTFLILLGCAIVLSGQKNFKAEEFTCRDGSILMYQKMEP
                     RKIKKSKKYPLVVFLHGAGERGADNKSQLVHGAKLFDDKKNRRKYPAFVLFPQCPSNE
                     YWAKVDIDRSDKEDPFRFDFRDKPNKPLESVFELIRSYLRLPSVDPDRVYIMGLSMGG
                     MGTFEAISRHPEWFAAAIPICGGGDLEFVESFAQKVPVWVFHGAKDDVVLPKYSRDIV
                     QEIIFKGGFPRYSEYSEANHNAWDKAFAEKDLLPWLFHQKKTLTN"
     gene            complement(7124..8164)
                     /locus_tag="PKV31_02435"
     CDS             complement(7124..8164)
                     /locus_tag="PKV31_02435"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNB91740.1"
                     /translation="MIHLDGSFISSLMIHRVGNKSLDEGFKLTDKPIRTDPELMAVLR
                     PYFTSMLSGDELFSFTNEVNLDQNEVYHLARKIFAEPSSLAEHSKAITAHLYNQSTHP
                     RIKSGELYVAYFEEVVIDDEVTSAIGIFKSESRETFLQVDFSSELAGISHFEGIPARR
                     PDKACMIFNVASNEGFRVAIFDHLNRTSEAGFWRDDFLNLKPVGTDFYQTNYMLDVTK
                     QFVTKRYVEEHEPSKVDQIELLNRSVDYFKNNETFDRGEFCTAVFDDKDVRTSFLKFE
                     EQQQSEGKMRADETFSISLPAVKKQSRSIRSIIKLDRNFHIYVHGDKDLIQQGVDENG
                     RKYYKIYYETES"
     gene            8359..8652
                     /locus_tag="PKV31_02440"
     CDS             8359..8652
                     /locus_tag="PKV31_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91741.1"
                     /translation="MDVISGNYDLTKAEAGPVMQAAFWDKQSDDDLPDSGPEFLYTLL
                     YRPVFDNSHNHSEITELSFGLYQYSQKLGMYIIKESRWHSDLSKGQIVLFVRI"
     gene            complement(8695..10272)
                     /locus_tag="PKV31_02445"
     CDS             complement(8695..10272)
                     /locus_tag="PKV31_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HNB91742.1"
                     /translation="MIKGINHIGYEAVAGTGPSFRAFNPTIGEFFGEEFFSASEEQIG
                     KAAELAGEAFHIYKNISPEKRAEFLEAIADAILDLGDSLLERAHQESGLPLARLTGER
                     GRTVNQLRMFADILREGSWVEAVVDTAMPDRQPLPRPDIRKMLLPIGPVAVFGASNFP
                     LAFSTAGGDTASSLAAGNPVIVKVHPGHPGTNELVASAILSAARKTGMPNGVFSSIIG
                     NRIETGVQLVKHPCVTAVGFTGSYQGGMSLIKTAAERPVPIPVFAEMGSINPVVVLPG
                     IIERETEMIAGQLAASVTLGVGQFCTNPGLIFIMEGGKSEDFISILMEKMAATPHGTM
                     LNQNVCQSYYQKRDELATKTDIATVLQGENLAGTGQGTSALYRIEGSRFISDDGLQAE
                     VFGPSTLVVVCRGQKELMEAIRHCHGQLTGTVMGISEELEQFRDCIAALQEKVGRLIF
                     NGVPTGVEVCHAMIHGGPFPASGNSQYTSVGADAIKRFVRPVCFQDCPEALLPNELKD
                     ANPKGIFRKVDGVWGRH"
     gene            complement(10276..11193)
                     /locus_tag="PKV31_02450"
     CDS             complement(10276..11193)
                     /locus_tag="PKV31_02450"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNB91743.1"
                     /translation="MAKPAWTGVYPALLTPFTKDDQIDLELFGKNLKAQIDAGVSGVI
                     IGGTLGEASTLEHGEIISMLEYAKSNVAADFPVVVNVAEGSTRNAIRAAQNIQAHGAD
                     GIMLLPPMRYKSDDAETVAFFSAVATSISLPVMVYNNPVDYKIEVTLPMFEQLAKIEN
                     IQSVKESTRDVSNVTRMKNTFGDRFKILCGVDTLTLEEIALGADGLVAGLVDAFPAET
                     VAIFNLMKAGRYEEALKIYRWFLPLLELDIVPKLVQYIKLAATQTGIGSEYVRAPRQM
                     ISGTERERVLDIINRGLANRPQLPDYRNL"
     gene            complement(12137..13852)
                     /locus_tag="PKV31_02455"
     CDS             complement(12137..13852)
                     /locus_tag="PKV31_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNB91744.1"
                     /translation="MDANFSINFRFRTTILMVFVFFIFSKMPIAQPVLQLGAPVITGL
                     SQPMQIVNAGDGTGRYFVVHKGGLIKCYDKDFNFLSDFLTVTNITSSGERGLLSMAFH
                     PDYKTNGLFYVYYTGNESGKVGALTIARYKVSTGNANLADVASKKIVLEILHPRTNHN
                     GGQLHFDQAGYLFLTTGDGGGGGDPDENSQDQTKLLGKLLRMEVNTSDTPPYYSVPTD
                     NPFGNEVYCLGLRNPYRWSFDRLNGDIWIGDVGQSNWEELDHVVQSQISGTNFGWDCY
                     EGNVSYEPSGCLPSSNYKFPFHVYSLSGAQSVTGGVVYRGAKYPSLYGWYVGAEFYNT
                     DFHRVFFDGTNYTVAPVQNLGPGSIVNFGEAEDGEVYVVSLYEGEIYPLQVDEGPLPV
                     RFITLGAHRMGLTNLIEWTAAEEEGSSHYEIESCMDGTTFKKIGYIHAKASGKSGIHS
                     YIFSDIPNSVGKMYYRVKAVDVNNSYRYSDIVTVVDERSRIIVVYPTLVQYDEICVYP
                     NSINDHIILELFNTNGQLMGQRTVAPGEREVRLPVNNATPGVYYVRVTSGHTIEMHKI
                     VKPMN"
BASE COUNT         3979 a         3195 c         2942 g         4054 t
ORIGIN      
        1 taccagaatc aggatttgat aaaaagtcat atttgctgaa atttcataaa tacatagcat
       61 gaattaatgt ataattcaaa ccaggaaata ataaccgact aatttttccc tgctaatata
      121 gtgcaataaa catacttttt ggagagacta catcaaatgg ggtaatgacc gattccgttt
      181 ttcttaaaat caacaaatat accgggccgt tataaagcgt gtgaatatta aaacaaaatt
      241 attatattaa tttgattata ttgtttttat atattacaaa tatttcataa taatcaggaa
      301 tattaaaaaa aagtagccct acaaaattag tataccgcgt taatttgagg ctattcatca
      361 ggtcatccaa ttcccttgac aacaaatgat ataagttaat gtgccaagat gagataaccc
      421 caattattag taaactccgg acaaggccgg tccgttgcaa gacttatctt cagcctgatc
      481 ctgattttta tccaataaat tgtgaaatac actttaccgt gcaaatgaac ccggatttgt
      541 tagaaataac tacctttgcc ctttaaaatt ataatccaat gtgtggtatc ctaggaatct
      601 tcgatctcag gcaaagtcct gattccctcc gtgctcaggc gcttgccatg tccagaaaac
      661 aaaggcatcg tggacctgac tggtccggca tatttacctg caacaatgcc atcctggcac
      721 acgaaaggct ttctattgta gaccctacct ccggcaagca accgttgttt agccaggacg
      781 gagcaattgt gctggccgtg aatggtgaaa tatacaatca ccgtcagttg aaaaaagggc
      841 tcacaacgcc atatcccttt cagacacaat ctgactgtga aataatcctt gctctgtatc
      901 aggagaaagg tatcgacttc atagaagacc tgaatggcat ctttgccttt gccttgtatg
      961 accacgccaa tgattcatat ctgatcgccc gagaccacat gggcatcatt cctctttaca
     1021 tgggatggga cgaatacggc aatttttatg tggcgtccga gatgaaggca ctgagtggta
     1081 tatgcaacaa gatccgggag tttttgccgg ggcactatct gtattccagg gatggggaga
     1141 tgaagaaatg gtataagcgc gactggatgg aatacgataa tgtcaaggac aatgaaacgg
     1201 atatccagaa gctgagaagc gcttttgaag aagctgtcca tcgccaatta atgtccgatg
     1261 tgccctacgg tgtactcctt tcaggtgggt tagactccag tattgtaagt gccatagcca
     1321 aaaagtacgc tgcacgaagg attgagacgg aggatctgaa ggacgcatgg tggccgcaat
     1381 tgcacagctt tgcagttggc ttgaaagatt ctcctgacct ggctgcatca agaaaggtag
     1441 ctgagcacat cggcactatt caccatgaga ttcactttac cattcaggaa gggctggatg
     1501 caatctccga tgtcatctat catattgaga cttatgatgt tactacgatt cgcgccagca
     1561 ctccgatgta ccttcttgcc cgggtcatca agtcgatggg tatcaaaatg gttctttcgg
     1621 gagaaggatc ggacgaaatc ttcggcggtt atttatactt ccataaggca cctgatgcca
     1681 gggctttcca tgaagaaacc gtaagaaaag ttggcaagtt gcacctctat gattgcctga
     1741 gggctaacaa gtcgttggct gcctggggtg tagaaggcag ggtgccattc ctggacaagg
     1801 agtttatgga tgtagccatg aggatcaacc caaaggacaa aatggccggt aacggaaaga
     1861 tcgaaaaata cattctcaga aaagcttttg aggactacct tcctcacgaa gtggcatgga
     1921 ggcagaagga acaattttcg gatggagtcg gttacagttg gatagacacg ctcaagttga
     1981 ttacggctga gagggtcacc gacgaacaat tgaataatgc tgcccataga tttcctctga
     2041 atactccgac gaccaaggaa gagtattatt accgttcctt atttgccgaa cacttcccgg
     2101 gaagtgaaga ggctgcctgt gtgccttttg aagcgtcagt ggcttgcagt accgctattg
     2161 cgctcgaatg ggatgaagca tttaaaaaga tgaaggatcc aagtgggcgg gcagtgtctt
     2221 cagtacatga agccggctac aaagaatgac atatcgcgag gaaattcctg cgatcaggtg
     2281 ctgaggattt taatttctac tctttgattt ttctttcgtc cttccggtgt tctgtcggat
     2341 gcaataggtt tgctttttcc ataacctttg taggtgactc tgtttttatc gatgcccttg
     2401 ttaataatat agttagctac ttccctggct ctggctgagg acaatttctg acagtactcc
     2461 acggagggga caccattggt atgaccacca atttcaattt ttaccgcttc attcctttgc
     2521 atgaagtcca ctacctcatt tagcaccttg tgactatttt tgtcaagtga agagttatct
     2581 gccttgaaat aaagctggtc cagtttgatg atttgtccgg gagtaacctt ctgagtcagt
     2641 tcctttacgg taacgggtga ctccttgatg acttccacga tgttgcttgc attgtcgacc
     2701 aggatattcc cattgtatgg aacaagggtg ggtgtcttgt agaaagcttc caagacgata
     2761 taattcacat tcttagtggg tttgaatgta aaatagtatt tcttccagtt attatttgta
     2821 ataagagagc tctcagccag caatacttcc ttactacaca ggctattccc gccccagatc
     2881 ctgagtttgg ccggagtagt gtagttggat tccgtacttc tgttggcttt ggtaaagctc
     2941 atatacactt cagatgtaca caaaaaaagg tcaaaagtat aggtcttgcc tttcttcaaa
     3001 gtacctgaca attgttgaga taccgactcc caggtatcat tctcgcgcac gaccattcca
     3061 agataggtat caccgttgtt tgccggcttg cgaacttccc acgatccact tggctgaaca
     3121 tcgggtgggc tctctcccgg aaataaaaaa gcaccacaat cccgccaacc aattggtgga
     3181 cgactatgac cgggaatatc ttcaaaggaa ggattagtaa ggcgaattac ttcctggctg
     3241 tatagattta ttgtcaggta catgaaaaat atacacccaa aaatcttccc agaatacttt
     3301 tgcaacctca aaaagataac tatcatggtc tattgtcaac agtatttcta aaaacgtccg
     3361 tcaacggtat ttagcctacc cggggatatt tttaacaaga gaataacagc gaaatcacaa
     3421 aaacagttca tattcactct tccttccgac ccgggggcga caccattgat gtcctaagtt
     3481 tgatatacca aattttcatg aaaaggcgtt gaaccctgtg acatccatgc ctgtaatcag
     3541 gaggtgaata tcgtgagtgc cttcatatgt aaggacagtc tcaagattca tcatatgcct
     3601 catgataggg tattcattcg taatgcccat gccgccatgt atttggcgtg cttctctggc
     3661 cacttccagt gccatatgaa cattgtttct cttcgccatg gatatctgtg ccggtgttgc
     3721 tttcccctga ttggcaagtg tgcccagccg ccatgccaat agttgtgcct tagttatttc
     3781 ggtgatcatc tctgccagtt tcttttgagt aagctggaat tggcccaggg gcttcccaaa
     3841 ttgaattctt tccctggaat atctcaaagc actgtcataa caatccagtg cagcgcccaa
     3901 tacaccccat gctataccgt aacgggcctt tgaaaggcat gaaagcgggc ctttcaatcc
     3961 tctgatatcc gggaagacat ttgccttagg cactctcaca tccgaaaaca caagttcccc
     4021 cgtgatagat gctctcagtg accatttacc atggatctca ggtgcactaa atccgggtgt
     4081 atccttttcg accaccatgc ccctcacgac gccttcctca tccttggccc acacaacagc
     4141 gatatctgct acaggcgaat tggttatcca catttttgaa ccattgagga tgaaggcatc
     4201 tccgtcatcc acgatgttgg tctccatgcc tccggggttg gaaccatgat ccggctcagt
     4261 aagcccaaaa caaccaatca gctcaccggt tgccagtttg ggaagatatt ttctcttctg
     4321 ttcttcgctt gcaaatcgaa atatgggata catcacaagg ctaccctgca ctgaggccat
     4381 cgatctgatt cctgaatctc ctcgctccag ttcggccata atgataccat aggcaatttc
     4441 gtccatgcct ccgcctccgt attctgccgg aatactgggg ccgaaagcgc caatttcacc
     4501 cagcccctta aaaagatgag tcgggcattc tgcacggttg gcataatctt cgatgattgg
     4561 gcttacttcc tgcttcaccc atgccctgac agcttcccgg gccagaatat ggtcttcggt
     4621 caacaattca tcgatagcat agtagtcatg ggattggaaa aggtcagatt tgtttgattt
     4681 gtggtgagtt gtcggcttat gatccatgtc ttttttgatt ttggctgcaa aaatactaaa
     4741 atttagtagg tagcctgtat tcgtaaagaa aaagattata aaatcgggat gagctccatc
     4801 cggaaaaagc agggactccc ttcaaggtga gattgattat acaataccat tgattgcaaa
     4861 tacaaaagaa cttttctata gtcccccaca agatagaata atgaatatat taagcttcaa
     4921 aatccctctt tcaggcagtc gccttgaccc cggtagactt tcgggtggat ttcttactat
     4981 tgccgctttc ttcctggcca ctgcctgcga tggctttgta tttcataatt tcttcttctg
     5041 aaacggattc aaagagggga tccaactcaa gagcattatt aaaagaaaga attgcctccg
     5101 catgctgatt ggtgtgcaaa tatgcctttc caaggctgaa tgtcaccatt cgcttccagt
     5161 aatgattcga attgcctttt tcaaaaggtc gcttggtaaa caatttccat tcaaagatgg
     5221 ccttttcaaa atctccaagg tgaaaataac atttgccttt caacctgcga cctatccagt
     5281 gaatattctg gtgtgcaata gaacactccg taaccttttc caaaacagga agggcctcct
     5341 tgtatctttc ttcacggata agcattttcc ctttccaaag atatgcttcc gtaaggcagg
     5401 gatcgagctc tgtggctaga tcaaacttct ttatagcaaa ggcaatattt ccctgtcgtg
     5461 ccgcaatcct ccctgcaagc gcatgagcca tggaatgtcc ttcgtatttg ctgatgatgc
     5521 catccaatat cttcatagct ttggaaaggt caccgttttc attgacaatt tcattggcct
     5581 tataaaagaa gcaacgatgc ttttatcgct ggaaggcctg atctgttcat ggcggattac
     5641 ctttccatcg accatcatcc acatctccac cctacccgta acagaatatt ccgccagatt
     5701 cttcaaaaga tgtactgtat ttcgccaata tttatcggag acaagattgt tgaaacgttt
     5761 gacaaagaat atcaggtttt cctcgtcaaa gcattcctcc atcttgttga aaatgacttc
     5821 attcttatag tagacatcct tccgttgtgt gtacaacttc agagctttgt cataggtatt
     5881 cgcgtttcca aagtccaacc gaccctgaac aataactttt gagaacataa aacaatacta
     5941 tggttagaat gtgctatcag aaatgcagca gcaatcacgc aacaacacct ccgctctgat
     6001 tcacacaaga aatacaaaga tacgattcgt aaaaattcaa agcaagtgtt tgtaaacata
     6061 ttatttattt atatataaca atgtcttatc tgaagtagaa attgtttaat atatatgaaa
     6121 ttattttata ttttatttat cggtatgact tgcggataac atatatatga actcaaaatc
     6181 tctctttccg tttttaaaaa tatttgaatg tctgattggg tatttttatg atctttgagg
     6241 aataattggc ttctgatatg aaaaatcttt tcttgacatt tctaatcctc ctgggatgtg
     6301 ccattgttct ttccgggcaa aaaaatttta aagcagaaga atttacctgc cgtgacggga
     6361 gtatattgat gtatcaaaaa atggagcctc gtaaaataaa gaaatccaag aaatatccgc
     6421 ttgtcgtatt tctacatggt gccggtgaac gtggtgctga caataagagt caactggttc
     6481 atggtgcaaa actatttgat gacaaaaaga acaggcgaaa atatcccgcc tttgtattat
     6541 ttccgcaatg cccatccaat gagtactggg caaaagtgga tatcgataga agtgataagg
     6601 aggatccctt ccgatttgac ttcagggata agcccaacaa gcccctggaa tccgtatttg
     6661 aactcatcag gagttatctc cggcttcctt ctgtcgatcc ggaccgggtt tatatcatgg
     6721 gcctttccat gggtgggatg ggtacatttg aagcaataag caggcatccg gagtggtttg
     6781 ctgctgcaat tccgatctgt ggtggcggag accttgaatt tgtagagtca tttgcccaaa
     6841 aggttcccgt gtgggtattt catggagcaa aggacgatgt tgtattgccc aaatatagcc
     6901 gagatattgt acaggaaatt attttcaagg gtggttttcc acggtactct gaatattctg
     6961 aagccaatca taatgcctgg gacaaggcgt ttgcggaaaa ggatctcctg ccgtggttgt
     7021 tccatcaaaa aaagaccctg acgaattgaa aagttttttt tacaactgca tgtgtatgaa
     7081 tcttgcactg ccaggcaaag tgaccaattt ctttccgtga taatcacgac tctgtttcgt
     7141 aatagatttt atagtattta cgaccatttt catcgactcc ttgctggatg aggtctttat
     7201 caccatgtac atagatatga aaattcctgt ccaatttgat gatgctgcga atacttcggc
     7261 tttgcttttt tactgcaggc aaggaaatgc tgaaggtctc gtctgccctc atctttcctt
     7321 cgctttgttg ctgctcttcg aatttgagga atgaagtacg tacatccttg tcatcaaaaa
     7381 cggcagtaca gaattctccc cgatcgaatg tttcgttatt cttaaaataa tcaacagaac
     7441 gattgagcaa ttctatctga tcgaccttgg agggttcatg ttcttcaaca taccgtttag
     7501 tgacaaattg tttggtgaca tcgagcatat aattggtttg ataaaaatcg gtgccgacag
     7561 gctttagatt gagaaaatca tccctccaga atcctgcctc tgacgtcctg ttcaggtgat
     7621 cgaaaatcgc cactctgaaa ccttcatttg atgccacatt gaagatcata caagccttgt
     7681 ccggccgtcg tgccgggatt ccttcaaagt gagagatacc ggcaagttct gatgagaaat
     7741 ccacttgcag gaaagtttct ctcgattcgg acttaaagat gccgatagct gaagtcactt
     7801 catcgtcaat gactacttct tcaaaataag caacataaag ctcaccggac ttgatacgtg
     7861 gatgtgtgct ctgattgtac agatgggctg taattgcctt gctgtgttca gcaagtgacg
     7921 atggttcggc aaatatcttt cttgccagat gatatacttc attctgatca agattgactt
     7981 cattggtaaa gctaaatagt tcgtcaccgg acagcataga ggtaaaatag ggtcggagga
     8041 ctgccatcaa ttccggatca gtccggatag gcttatccgt aagtttaaat ccctcatcaa
     8101 gagatttatt acccactcgg tggatcatca gggatgaaat aaaactgccg tctaaatgta
     8161 tcataccgga atcaaattta taatttatat cggacttccc gcttaggaag acaatccaaa
     8221 aataaaaggg ctttgataat ttttcctgaa aatataaata tgcaatttga aaactttgcg
     8281 gttataacaa agataacgcc cacaattgcc tatatgatat aattgccgga aaagataccg
     8341 gctgattaat tgcgcttagt ggatgtaatt tccggtaatt atgaccttac aaaggctgag
     8401 gcaggtccgg tgatgcaagc agctttttgg gataagcaat ccgatgatga tctccctgat
     8461 tcaggaccag aatttctata taccttgtta tatcggcctg tatttgacaa tagtcataac
     8521 cattctgaaa tcacagaatt aagctttggc ctttatcaat actcccaaaa gttgggtatg
     8581 tatattatta aggaatcccg gtggcattct gatttatcca aagggcaaat tgtgttgttt
     8641 gtccgaatct gatattccgg taaggtcaac ctgtatttac cgtataatgc ccaatcaatg
     8701 tcttccccac actccatcga cttttctgaa gatgcccttg ggatttgcat ccttcaattc
     8761 attgggaagt aatgcctccg gacaatcctg aaagcacacc ggccggacga aacgttttat
     8821 tgcatcagca ccgactgagg tgtattggct attgccggat gctggaaacg gaccgccgtg
     8881 gatcatggca tgacacactt ctaccccggt aggcacgccg ttgaatatca gccggccgac
     8941 tttttcctgc aaagcggcga tacaatccct aaactgttcc aactcctcag atattcccat
     9001 taccgttccg gttaattgtc catggcagtg acggatagcc tccatcaatt ctttctgacc
     9061 gcgacagaca actacgaggg tcgatggtcc gaaaacttct gcctgcaagc catcatcact
     9121 tataaaacga cttccttcga tcctgtaaag cgcagatgtg ccctgtccgg tcccggccaa
     9181 attctctcct tgcagcacag tggcaatgtc ggttttagtg gccagttcat cgcgtttttg
     9241 ataataggat tggcaaacat tctgattgag catcgtgcca tgaggtgtgg cagccatttt
     9301 ttccataagt attgaaatga agtcttcgga ttttcctcct tccatgatga agatcaaacc
     9361 cggattggtg caaaattgac ccacgcccaa agtaacagaa gcagcaagtt gccctgcgat
     9421 catttctgtc tctctttcta ttataccggg aaggacaacg accggattga tgctgcccat
     9481 ctcagcgaaa acaggaatcg gtacaggcct ctcagcagcg gtttttatca ggctcattcc
     9541 cccctgataa gaaccggtaa agcccacagc agtaacacaa ggatgtttga caagctgaac
     9601 acctgtctca atccggttgc cgatgatgga agaaaaaaca ccattcggca tacctgtctt
     9661 ccgggcagct gataggatcg ccgatgcaac cagttcgttg gtgccgggat ggcccggatg
     9721 aaccttaacg attaccggat tgccggcagc cagtgaactt gcggtatctc ctccggcagt
     9781 tgaaaatgcc aggggaaaat tgcttgcacc aaataccgca acgggaccta taggtagtaa
     9841 catttttcgg atatccggtc ttggaagtgg ctgcctgtcg ggcatcgcgg tatctaccac
     9901 tgcctccacc caggaacctt ccctcaatat atcggcaaac atccgcaatt ggttgaccgt
     9961 cctgcctctt tcgccggtca gtcgcgcgag tggcaggcct gattcctgat gtgcccgctc
    10021 aagaaggcta tctccgaggt caagtatcgc atcggcaatt gcttccaaaa attcggcacg
    10081 cttttcagga cttatatttt tatatatatg gaaggcttcc ccggccagtt ctgcggcttt
    10141 cccaatctgt tcttcactgg cagaaaaaaa ttcttctccg aaaaattcac cgatcgtggg
    10201 attgaaggcg cggaaggaag ggcctgtccc tgctacggct tcgtatccga tatgattgat
    10261 tcccttaatc atgttttaca gatttctata atcaggtaat tgaggtcgat tagctaatcc
    10321 ccgattgatg atgtcgagca ctctttccct ctctgtaccg gaaatcatct ggcggggagc
    10381 gcggacatac tcgcttccga tacctgtttg agtggctgcc agtttgatgt actgtaccag
    10441 cttgggtaca atatcaagct cgagcaatgg caaaaaccat cggtatattt ttaacgcctc
    10501 ttcatatcgt ccggctttca tgagattgaa gatagcgaca gtttctgccg gaaaagcatc
    10561 caccaggccg gccaccaggc catcagcgcc cagggcaatt tcttccagcg taagtgtgtc
    10621 cacgccgcac aatattttga atctatcacc aaaagtattt ttcattctgg tgacattcga
    10681 tacgtcacgg gtggattcct tgactgattg gatattttcg attttagcaa gttgttcgaa
    10741 catgggaagt gtcacttcta tcttatagtc tacaggatta ttgtatacca ttaccggtag
    10801 cgagatggaa gttgccaccg cactaaaaaa tgctacagtc tctgcgtcat cggatttata
    10861 tcgcatgggt ggtaataaca tgatgccatc ggctccatga gcctggatgt tttgagccgc
    10921 acggatagca ttgcgtgtag agccttcggc aacattgacc actaccggaa agtcggcagc
    10981 gacattggat ttagcatatt ccagcattga aatgatttcg ccatgctcga gcgtacttgc
    11041 ctcacccagg gttccgccaa tgattacgcc ggaaacacct gcgtcaatct gtgctttgag
    11101 atttttgcca aatagttcca gatcgatctg gtcatctttc gtaaagggtg tcaaaagtgc
    11161 gggatacacg cccgtccaag ccggttttgc catagtttaa atgctgtttt attttgggtc
    11221 aaaggtatga cgatgcagaa aatcaaccta aacgttagat agccgattat ttacctattt
    11281 taacccattt tatatattta ttcaataaaa tgggtgaatt attgttttac atccggggga
    11341 tattaaccac atataaataa ggtaaaattt ggctacagca tatacttata tatcccagga
    11401 gtctcaccct tgcttataaa atttaaaaat aagagaacga gggcctcgtc cacttcattc
    11461 ggataatcaa aagacttttc agcacggatg aaagctacaa cgcgtagtgg catcagagaa
    11521 caatcacgta tatattgaag tcagatgatt ctgtcaaaac tcttgtgaaa caattcgatt
    11581 aaagaaatga tatcttgtcg cggtccggta gtccgaagac atcgattctc ttctacgctt
    11641 tatacacagt atcaccaagg gcttcgctga acaaatcact aaaggtgact tacgctcaga
    11701 tcaatatagt atgtgcactc aatatatcca accggtaatt attagagact gcattgtcat
    11761 cgaccggaac atttttgaaa atcaatatga gggcgaccag ttataatagc tgtgctaaag
    11821 gcaggtatca ggaggaggtc gcctgtaaaa tttctccagc atgttaatga ttgaaataaa
    11881 taacgttgat gcagtgacaa ggaatatgcc tacagattgc atatgccttc tcaggaatac
    11941 aacattacca aatgaccaaa tgcaataggc ttgtttgaaa agaaagggcg ttcaaacaaa
    12001 acagcaaaat gccggatgcg taggaaggat actcaattgt tccgtcactc tgaaatcttt
    12061 ctatacacat agcctacttt aggctgtgtg aatgatctgt cccgggaagc gctcaaatca
    12121 cccattcata ttccaatcaa ttcataggtt tgacaatctt gtgcatttcg atggtgtgac
    12181 ctgaggttac acgcacataa taaactccgg gagtagcatt atttactggc agcctcacct
    12241 ctctctcccc gggagcaacc gtcctctgac ccatcaattg tccatttgta ttgaatagct
    12301 ccagtataat gtggtcatta attgagttgg gataaacaca gatctcatcg tattgcacca
    12361 atgtagggta cacaacgata atcctacttc tctcatcaac cactgtaact atatccgaat
    12421 acctgtaact gttattgaca tccacggctt taactctata atacattttc ccgacagagt
    12481 tgggtatatc gctgaaaatg taagaatgga taccggattt tccgcttgct ttcgcatgta
    12541 tatatccaat ttttttgaag gtagttccat ccatgcagga ctcaatttca taatggctac
    12601 ttccttcttc ctctgctgca gtccattcta tcaggtttgt caggcccatt ctatgtgcac
    12661 ctaaagttat aaagcgtacc ggtaagggtc cctcatccac ctggagaggg taaatttcgc
    12721 cttcatacaa ggagactaca tatacctcac catcttctgc ctcaccaaaa ttgacaattg
    12781 atcccggacc aagattctgc accggtgcaa ctgtataatt tgtcccatca aaaaataccc
    12841 ggtgaaaatc cgtgttataa aattccgctc cgacatacca accatacagg gatggatatt
    12901 tggctccccg atatactacc cctcccgtaa cactttgcgc tccgcttaat gaatatacat
    12961 gaaagggaaa cttatagttg gaactaggca gacaaccgga tggttcataa gatacattac
    13021 cttcatagca gtcccaaccg aaattagtac cactgatttg tgattgaacc acatgatcca
    13081 actcctccca attgctttgc cccacatctc ctatccaaat atcgccattg agcctatcaa
    13141 agctccaacg atatggattt cgcagtccca gacagtagac ttcattgccg aatgggttgt
    13201 cagttggaac agaataatag ggtggtgtat ccgaggtatt aacttccata cgcaacagtt
    13261 tgcccagtaa cttagtctga tcttgcgagt tttcgtccgg atcgcctcct cccccaccat
    13321 caccggtagt gagaaacagg tagccagcct gatcaaaatg caactgccca ccattgtggt
    13381 tggttctggg atgtaaaatt tccagaacta tcttttttga tgcaacatcc gccaaattgg
    13441 catttcctgt actcacttta tagcgagcta tagtaagtgc acctacctta cccgattcat
    13501 ttccggtata atagacatag aataagccat tagtcttata gtccgggtga aatgccatac
    13561 tcaaaagacc gcgctcccct gagcttgtaa tgttggtcac ggtcagaaaa tcagaaagaa
    13621 aattaaaatc cttgtcataa cacttgatga ggccaccttt atggactaca aaatatctcc
    13681 ctgttccatc accggcattt acaatctgca tgggctgaga aaggccggtg ataaccgggg
    13741 cccccaattg caatacgggc tgagcaatag gcattttcga aaaaatgaaa aatacgaata
    13801 ccatcaaaat ggtggtcctg aagcggaaat tgatagaaaa attagcatcc atacaacata
    13861 cctttagagg ttaaaaaaga ttagagcaat gatgcttagc agcgtgtgaa cttttttcga
    13921 taattcgaca gaatcccaaa ggaaggattc tctcggttcc aattatttac ttaggtctaa
    13981 ctaatgaata aagaggctta atgctacaaa gataataaat taacttgtta aatcaaaaac
    14041 acacctcatt tttttcatcg aaaattcaag gccctgtata tctttcttaa gatgtacctg
    14101 gacacaatac gcaccggaaa atacgagcat tacaactaac cattccgatg ggaatttcct
    14161 ctttcaagat
//
LOCUS       DAOQMC010000033        13981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1610_length_13981_cov_5.435516, whole genome shotgun sequence.
ACCESSION   DAOQMC010000033 DAOQMC010000000
VERSION     DAOQMC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13981
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1610_length_13981_cov_5.435516"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKV31_02460"
     CDS             <1..470
                     /locus_tag="PKV31_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNB91745.1"
                     /translation="RGEFTVANAGDVGYIYHPRKYFTNQSEDAPFGDPNPNGESNDRV
                     IRYADVLLMDAEAAAKTGDETKARESLNRVRMRVGVPAITASSSALLDAIFRERRIEL
                     GLEGHRFFDLVRTGRAAEKLGPLGFKDGVHNIFPIPNSEIVLSNGILKQNPGY"
     gene            488..1321
                     /locus_tag="PKV31_02465"
     CDS             488..1321
                     /locus_tag="PKV31_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91746.1"
                     /translation="MMKNIFIVIIGLFIFQGCSPDNNDINLGPLPPKPDFSIEYVPGD
                     SNKVVVKDLSSDSFVRLWEIGEGGIPATSSLESDTIFFSKKGEYDISLYISQKGGNGT
                     AYNTKKISIPKDVVLPCSGPLGLLTGNCSTTGKCWKFSDVAGAITVGETYGAANWYKS
                     PANGLVASQYDDRYCFVFEGFKFDYRNNGSTVNPFKGYVDEPFIPVSGPFAFSPGTGP
                     NGEDQIILAKGQFMGTMDSYSVLNIIKLTETELIVRAQFADAAGNPVTPGWFEFKYVA
                     D"
     gene            1321..2112
                     /locus_tag="PKV31_02470"
     CDS             1321..2112
                     /locus_tag="PKV31_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNB91747.1"
                     /translation="MQKLFFIFLFLSAWLVSFGQYGRLVWQDEFESQSPVPDSTKWTF
                     DLGDGCPSNCGWGNNQKEYYTNDLRNARIEEGRLILEAHRNNRSGHPYTSARITTRKS
                     LNLRYGRIDVRARLPRGHGTWPAIWMLPSKWEYGGWPRSGEIDIMEHVGWAQDSIFGT
                     VHTQNYNHIIGTQQGKTVQLPDASLYFNDYSIIWTEEKIDFLINGKVFYTYKNDSTGP
                     DSWPFDKEFYLIMNLAIGGNFGGVKGVDDDIWPQRMEVEYVRVYN"
     gene            2119..3324
                     /locus_tag="PKV31_02475"
     CDS             2119..3324
                     /locus_tag="PKV31_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNB91748.1"
                     /translation="MNRFRKISYAWLWPLLFLNASCDKPNDQPSVVKPEVTIEDQNGL
                     EKNEDFIALISIKLSKPATTNVVLRYSTKDITARSGQDYIGVTDVLSIIPAGQSGVLV
                     PVTIIGDKVKEPDETFELVITQALNATIVKQRALITIINDDADSNIIEVPTEGYVSPG
                     EYPGYQLVWSDEFRQPTIDKSNWTFEIGNGSNGWGNNELQYYKEENASIYAEEYLMIT
                     AKEESVGGFDYTSSRMITKGKREFKFGRVDIRAAMPIGQGIWPALWSLGADIQTNPWP
                     NCGEIDIMEYLGHESSRVHGTVHWGLNTHKYKGSSTLSGNEGDFHKAFHVFSIIWEKD
                     KIEWYVDDKKYFSISASEMEGQPYPFNNDQFFIINCAVGGNWPGSPNNSTIFPQRFFV
                     DYIRVFQKT"
     gene            3399..6407
                     /locus_tag="PKV31_02480"
     CDS             3399..6407
                     /locus_tag="PKV31_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB91749.1"
                     /translation="MRLKLTKQWWQMGWLVVLSLYSSGMTYAADVPPDYFYAYQNNTI
                     TVEGTVTGSDGMPVAGATITVKGQNEGTVTDADGRFLIKVAEDAILVVSSVGYVSVEV
                     KAASVVNVVLQVDNLLLNQVVVVGYGSQSKKNITGSISSVEGEEFANRPVFSTAQALQ
                     GKAAGVQVTQPSGKPGTEFSIRIRGTNSVNANNDPLYVIDGIPTTDTRGLNPNDIESM
                     QILKDAASAAIYGARASNGVVLITTKKGSAGSTATTFNTYFGFTRLAKSIDVLNGSQY
                     IEYMRDRGEAISEENANTDWEKETFGTGINQNYQLAFSGGTEQARYYLSLGYLGDKGM
                     VRPAEFKRYSARLNVDSRINKWLTVGSNINFYRTNTYNAGDNASSGRGGIILSALNSP
                     PNLSVYDTDNPKQFSTNPFQASWETPLSYMSREDINNSNRFLGNAFAEIRLMKDLKFR
                     TNLAADISNGVNEFYVDPVRTVYGRQNHGISNVSKWEYMSLLAENTLTYTNNFGLSNV
                     TALVGLSVQDNNYGFAYINTKDLPLEPVKTTNAGNQVEGAGNTKTTSAIVSQFGRLMY
                     DYDSKYLLTVAVRRDGSSKLAAGNKWDIFPSFSAGWRISQEEFFKNIHGVNDLKLRGG
                     WGQTGNQEGVSDFAAYGLLGFSRITPGNVFIGPGIRRLTIANPELRWEKTTQTNIGFD
                     ITVLENRLTMAADVYWKKTHGLIMSIPLPSSVGVEQPFIRNDGELENKGVEFMISSIN
                     FKKEKFTWNTDFNISFNKNKVSKLGLQKIYDYAKIYSNGQNAIRMTEGQPMGSFYGYI
                     SEGVDPESGNIKYRDLDGNGIITPDDRTFIGQASPKFIYGMTNNLSFMGFDLSIFIQG
                     VQGNKIFNATKMDLEGMFDSKNQSIRVLDRWKNPGDITSLPRANEAGDGSLDNIHNST
                     RFVEDGSFLRLKAVTLAYNIPTGWLKRANIRNLSIYATAQNLLTLTKYSGFDPEVNAY
                     GVNSRGTEFGVDYGTYPQTRQIIFGLNLGL"
     gene            6441..7883
                     /locus_tag="PKV31_02485"
     CDS             6441..7883
                     /locus_tag="PKV31_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNB91750.1"
                     /translation="MMKKKIYLAIAMFSGLFLTSCDSILDKEPLSELIDKTTDTDKPF
                     TAADAEAAMAAAYAGLKTSQAELMMLDYYVNGDAQSDNSHAGADNPDNFQIDDYKISS
                     TNNNVSRDWRYFYGLVALCNTVIEKAPTAADLTPARRDEMIGEASFLRGWLYFGMVQL
                     WGGVPIVTKPVPAISAENFEEVYKILYPDRKSLEEVYAQIIEDLVRAVNFAPTAYGPN
                     KMRGTKYAAHAVLAKVYATINPHDWAKVNEHCDAVIAGGFSLLPVYDHLWDGNHENSA
                     EAIFEMNCTDWNTGGNWGSSMFVGTDWKKFNTPTHNLVDAFDAAGDNVRKASSIKFQK
                     VTSWTDAYWDTNNYPFCFKMRNTAGGQNQIFLRLADIYLLKAEALTELGQLDNVSGAQ
                     YYVNLVRSRVSLTPTTATTADALRTAIANERRLELAFEGHRWYDLKRTGKAVETMSAL
                     GYNINENKLLWPIPQGERDKNINLTQNPGY"
     gene            8028..9461
                     /locus_tag="PKV31_02490"
     CDS             8028..9461
                     /locus_tag="PKV31_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HNB91751.1"
                     /translation="MDMRIVRQPLYVLLMLALFSFKCSKDDQPDTPVDVPVSDVQFYQ
                     TSADGSSLLQKQKQTLKFSNKTNQYPILKVDSTQQFQELDGFGYSLTGGSAIVLNTLS
                     ASDRNKILRELFSRDSSALAISCLRISIGASDLDPAPFSYDDVPEGQTDINLEKFSIE
                     PDRTNLIPVLKQILEINPEIMIMGSPWSAPPWMKTNKNFKGGSLKKEYFPVYARYFVK
                     YIQAMKAEGINIHAITVQNEPLHPGNNPSMLMEAQDQRDFVRDYLGPAFENAGITTKI
                     IIYDHNCDKPDYPISILNDATAKKYIDGSAFHLYAGEISAMSNVHEAHPDRNLYFTEQ
                     WTGKNESFASNFMWHTQHVVIGSLRNWSRMVLQWNLANDPGFGPHTDGGCSECKGALT
                     IGGTITRNPSYYILGQISAWVPPGSVRVASDNLPGLQSVAFLTPTGRKVLLVLNTTQT
                     NQSFNIEFKDRRVTTLLKAQSAGTYIW"
     gene            9480..10970
                     /locus_tag="PKV31_02495"
     CDS             9480..10970
                     /locus_tag="PKV31_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HNB91752.1"
                     /translation="MNKMAKNLIVLCILTVTFPLVTSAQGNVAVLSPATEIGKSGRAF
                     IYTTAFNTKLRLTRSGISEFRPKGQPLETEICVFVDPNKKFQEYIGVGGAITDASAET
                     FAALPSERQKELITAYFDREKGIGYNIIRTNINSCDFSSGSYTYVKDKDVTLESFDIG
                     HDLKFKIPMIKEAQRIIGNGMRFFASPWSPPAWMKDNNSMLHGGKLLPEYRDSWARYY
                     VRFIKDYEQQGIPVWGISIQNEPMATQRWESCIYTAEEERDFLKKHLGPVMEQEKLGD
                     KKIIVWDHNRDLIYQRAQTYMSDPEAARFIWGIGFHWYEDWSGGTPMYDNLQRVYEAF
                     PEKKLFFTEGCAESFRQDGYNNWELGEEYGRSMIRDFNNGMVGFTDWNILLDENGGPN
                     HVKNFCFAPVHGDTRTGQLIYTNAYYYIGHFSKFIEPGARRITASSTRSPLLSTAFEN
                     PDGTLVVIVMNQGDKDMDCLVWIDGAAAEANIRGRSINTILVPPAR"
     gene            complement(11015..11758)
                     /gene="fabG"
                     /locus_tag="PKV31_02500"
     CDS             complement(11015..11758)
                     /gene="fabG"
                     /locus_tag="PKV31_02500"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNB91753.1"
                     /translation="MKRLENKIAVITGGGSGIGRATALRFADEGAHVIIWDVADKKGE
                     EAITALKDKGSNAAYYHVDTTDKEQIAKATEDVISRYGRIDILINNAGITKDSTLLKM
                     TDEQWERVIDVNLNGVYHCTRAIAPIMAQNNYGRIINASSVVGLFGNFGQTNYAATKA
                     GLIGMTQTLSKELGRKGITVNAVAPGFIATEMIQLMPEDVLAGMEAKVPVKRLGQPEE
                     VAALYAFLASDEAAYINGAVIRIDGGIVL"
     gene            12024..13505
                     /locus_tag="PKV31_02505"
     CDS             12024..13505
                     /locus_tag="PKV31_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNB91754.1"
                     /translation="MDLKQATILLLAVYAAILLFLVYRGARKTRTLKDYAIGSGFPAI
                     VVAFSLAASVSSAATFIINPGFIAYYGWSAFLAMALVLPLGLYLSLIYLSKRFQHHGN
                     FTSALTLPQWAGNRFNSKVLERLLAIFSLLMITFLVLICVGLAKIISNSLGADEVSVM
                     IGVVVFVFGYMMFGGANALVYTNFVQAVLMLIVAVILIGSGYGYFEGGVGDYYDRLNT
                     IDPMLTKAFNPTSPLFRDWFEVIFCNFVVGIAIVCQPHIITKSLLLRSEKDINKYLFY
                     SVVVLIVFFSVVLAGFYGRMMFPDLKLDGSTINLDSIMSLYVVRRFSAGVGVLVVFGL
                     LAAGMSTLESLVQSLSITITQDLVRPALGKKASKVNLKVVNRFVIAFLALVTILVSYN
                     QMVNPTLSVGILAQNGVYSFFSAAFIPVLFGVFVKDAPKSSVIAASIVAIVVHFSVYY
                     LGLTPYTSGAVRNPAVASALAIVAAFLTGTTLLLIDRKKRIIE"
     gene            13502..>13981
                     /locus_tag="PKV31_02510"
     CDS             13502..>13981
                     /locus_tag="PKV31_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNB91755.1"
                     /translation="MNNAKIIASGWYAPSRIVDNQYFNEILGEDVDTWLREKVQIYER
                     RWCPDEESVADLAEQAIRQAIDHAGIRPDQVDLLIIATDTPEFISPSTASVIQDRLHL
                     TNAGTFDLNTACAGFVTALDVGSKYIQADNQYNNVVVAGVYAMSKYLNLQDKKTVTLF
                     "
BASE COUNT         3909 a         2968 c         3452 g         3652 t
ORIGIN      
        1 atcggggtga atttacggtt gccaatgccg gagatgtagg ttatatctat catccaagaa
       61 aatacttcac caatcaaagt gaagatgctc cttttggcga tcccaatccc aatggcgaga
      121 gtaatgaccg cgtcatcaga tatgccgatg tcctgttgat ggatgcagaa gcagctgcta
      181 aaaccggaga tgaaaccaag gcgcgagagt cactgaaccg ggtaagaatg cgggtaggtg
      241 tgccggctat tacagcttca agcagtgcat tgctggatgc tatttttcgg gaaagaagaa
      301 ttgagctggg cctggaaggg catcgtttct ttgacctggt acgcaccgga agagcagctg
      361 aaaaattagg cccactgggc tttaaggacg gggtacacaa tatattcccg ataccgaata
      421 gcgaaatcgt tttatccaac ggtatattga aacaaaatcc gggttattga ttcaatcaaa
      481 ttcattcatg atgaaaaata tatttatagt tataatagga ctcttcattt ttcaggggtg
      541 cagcccggat aacaacgaca tcaatctggg gccgttgccg ccaaagccgg atttctcaat
      601 tgaatatgta cccggtgata gcaataaggt tgtggttaaa gacttatctt ctgatagctt
      661 cgtacgctta tgggaaattg gcgagggagg tataccggca acatcatctc tggagagtga
      721 taccattttc ttttccaaaa agggagaata tgacatttcc ctttatattt cccaaaaagg
      781 cggtaatggt actgcataca ataccaagaa gatatctata cccaaagatg ttgtgcttcc
      841 ctgcagcgga ccacttggcc tgttgaccgg caattgcagc acgaccggca aatgctggaa
      901 attttccgat gtagcaggag ccattacagt aggagaaaca tatggcgctg ccaattggta
      961 taaatctccg gctaatggtt tggtcgcttc ccagtatgat gaccgatatt gttttgtctt
     1021 tgagggtttc aaattcgact atcgcaataa tggcagcacg gtcaatccgt tcaagggata
     1081 tgttgatgag ccctttattc ctgtttcggg tccgtttgct ttcagtcctg gcacaggacc
     1141 aaatggtgaa gatcagataa tcctggccaa agggcagttt atgggcacga tggatagtta
     1201 tagtgtacta aatattataa aactgacgga aacagaattg attgtcaggg ctcaatttgc
     1261 tgatgcggca ggcaatcccg ttacaccggg ctggtttgaa ttcaaatatg tggcagattg
     1321 atgcagaagc tcttctttat attcctcttt ttatcggcct ggcttgtgtc atttggccaa
     1381 tatggaaggc ttgtatggca ggatgaattt gaaagtcaat ctcccgtgcc ggattcaacc
     1441 aaatggactt ttgacctggg cgatggctgc ccatcaaatt gcggatgggg caacaatcag
     1501 aaagaatact atacgaatga cctgaggaat gcgcgcattg aggagggacg attgatcctg
     1561 gaggcgcacc ggaataaccg aagcggccac ccttatactt cggcgaggat aacaacccgc
     1621 aagtcactca atttaagata tggccgcatc gatgtcagag cacggcttcc acgtggacat
     1681 ggcacctggc ctgctatctg gatgctaccg tcaaaatggg aatatggagg gtggccccga
     1741 agcggtgaaa ttgatatcat ggaacatgtg ggatgggcac aggattctat tttcggaaca
     1801 gtacatactc agaattacaa ccatattatc ggtacacaac aggggaagac ggtgcaactg
     1861 ccggatgcat cactttattt caacgattat tccatcatat ggacagaaga aaaaatcgat
     1921 ttcctcatca atgggaaggt tttctacacc tataaaaacg acagtacagg tccggatagc
     1981 tggccttttg ataaggaatt ctatttaata atgaaccttg ccataggagg taatttcgga
     2041 ggagtcaagg gcgtagatga tgatatttgg ccgcagcgaa tggaagtgga atatgttaga
     2101 gtgtataatt gatggcatat gaatagattt agaaaaataa gttatgcttg gctttggcct
     2161 ttattgttcc tgaacgcaag ttgcgacaaa cccaatgatc agccatcggt agttaagccg
     2221 gaggtgacca ttgaagacca gaatgggctg gaaaaaaatg aagactttat tgccctgatc
     2281 agcatcaagc tgagcaaacc ggcaactacc aatgtcgtat tgagatacag caccaaagat
     2341 attactgcca ggagcggaca ggattacatc ggcgtgacag atgtgttgtc aatcattccc
     2401 gcagggcaat caggcgtgct tgtgccggtg accatcattg gggataaggt aaaagagccc
     2461 gacgaaacat ttgaactggt catcacccaa gctctcaatg caacaatcgt gaagcaacgc
     2521 gctttgatta ctattattaa tgatgatgca gacagcaata tcatagaggt gccaacagaa
     2581 ggatatgtct ctcccgggga atatcccggg tatcagctgg tttggtccga tgaatttcgt
     2641 cagccgacta tcgacaaaag caactggact tttgagattg gtaatggctc aaatggttgg
     2701 ggcaacaacg aattgcaata ttataaggag gagaatgcat ctatatacgc tgaggagtat
     2761 cttatgatca ctgccaagga agagtcggta gggggattcg attatacatc ttccaggatg
     2821 attaccaagg gcaagaggga gtttaaattc ggcagggtag atataagagc tgccatgcca
     2881 atcggacaag gtatttggcc tgccttatgg agcctcggtg cagatattca gactaatcct
     2941 tggcccaact gtggcgaaat agacatcatg gagtatcttg gacacgaatc ttccagggtg
     3001 catggaacgg tacattgggg attgaatacc cataagtaca aagggagcag caccctgtcg
     3061 ggaaatgaag gcgattttca caaggctttt cacgtttttt cgatcatctg ggagaaagat
     3121 aagatcgaat ggtacgtcga tgacaaaaag tatttcagca tatcggcatc tgaaatggag
     3181 ggccagccct atccgttcaa caatgaccaa ttcttcatca tcaattgcgc agtcggtggc
     3241 aattggccgg gaagtcctaa taattcaacg atatttccac aacggttttt tgttgattat
     3301 attcgggtgt ttcagaaaac ctgattgaat acgaaatggt aaatattgat aatgaataga
     3361 tcaaacctaa attaaataaa tcgaaaaaca acgtaagcat gagacttaaa ctaacgaagc
     3421 aatggtggca aatgggatgg ctggttgtac tttctctgta cagcagtggc atgacatatg
     3481 cagcagatgt gccgccagat tacttttacg catatcaaaa caacaccatt acagttgagg
     3541 gcacagtgac aggatctgat gggatgccgg tagccggagc gacgatcacg gtcaaaggac
     3601 agaatgaagg taccgtcacc gatgctgatg gacgtttttt aatcaaagtc gccgaagatg
     3661 ccatacttgt cgtttcctcc gtaggatatg taagtgtaga ggtaaaagcg gcgagtgtcg
     3721 tgaatgtcgt ccttcaggtg gacaacctcc tgttaaatca ggtagtagta gtcggttatg
     3781 gtagtcaaag taagaagaat atcaccggct ccatatcatc agttgagggc gaggagtttg
     3841 ccaatcggcc tgtattctca acggcacagg ccttacaggg gaaagctgcc ggagttcagg
     3901 tgactcagcc ttccggtaag ccgggcaccg aattttcgat tcgtatcaga gggacgaatt
     3961 cggtcaatgc caataatgac ccattgtatg tcatcgacgg cattcctaca acggacacac
     4021 gtggcctcaa tcccaacgac atcgaatcca tgcagattct taaagatgct gcctcagccg
     4081 cgatatatgg ggcccgtgcc tccaatggag tggtgctcat cactaccaaa aaggggagtg
     4141 ccggcagcac agctacaacc ttcaacacct attttggatt tacacgcttg gcaaaatcca
     4201 ttgatgtact taatggcagt cagtatattg aatatatgag agaccgggga gaggcaatca
     4261 gtgaagagaa tgccaatacc gattgggaaa aggagacctt cggtacaggc ataaaccaga
     4321 attatcagct tgctttttct ggtggtacag agcaggcgcg atattatctg tcgttgggat
     4381 atctgggaga caaaggaatg gtaagacccg ctgagttcaa gagatattct gccagactga
     4441 atgtcgattc caggatcaat aaatggttga ccgtggggtc taacatcaat ttttacagaa
     4501 ccaacaccta caacgccggc gacaatgcca gttcaggtcg tggtggcatc atactcagcg
     4561 cattgaacag tcccccgaat ttgtctgtgt atgacacaga taacccgaag cagttttcaa
     4621 ccaacccatt ccaggcttcc tgggagacac cactttctta catgagccgt gaagatatca
     4681 ataacagcaa tcgtttcctc ggaaatgcat ttgcagagat caggctgatg aaagatctga
     4741 aattcaggac taatcttgct gcggatatct ccaatggcgt caatgaattt tatgtcgatc
     4801 cggttagaac ggtatatggt cgtcagaatc acgggatatc caatgtatcc aagtgggagt
     4861 atatgagcct gcttgcagag aatacactta cttataccaa taacttcggg ttgtccaatg
     4921 taactgcact tgtggggctt tccgtacagg ataacaatta cggatttgca tatattaata
     4981 ccaaggacct gccactcgaa ccggtgaaga caaccaacgc cggaaatcag gttgaaggtg
     5041 ccggaaatac gaagacaacc tcggctatag tttcccaatt cggtaggctt atgtacgact
     5101 atgactccaa atatttactg acggtagctg ttagacggga tggatcttca aaactggcag
     5161 cagggaataa gtgggatatc ttcccgtctt tttcggccgg atggagaatt tctcaggaag
     5221 agtttttcaa aaacatacat ggagtcaatg acctcaaact acgcggcgga tgggggcaga
     5281 caggcaatca agagggggta agtgattttg ccgcatatgg actactcggc ttctccagga
     5341 ttactcccgg caatgtattt attggtcccg gaatacggcg gcttaccatc gccaatcctg
     5401 agcttcgctg ggaaaaaacg acccagacaa atatcggctt cgatataaca gttcttgaaa
     5461 accggttgac gatggcagct gatgtttact ggaagaaaac ccatggcctg atcatgtcca
     5521 tcccactgcc gtcaagtgtc ggggtagaac agccatttat acgcaatgac ggcgagcttg
     5581 aaaacaaggg ggtagaattc atgatttcca gtataaactt caagaaagag aagtttacat
     5641 ggaatacgga cttcaacatt tccttcaaca agaacaaggt ctctaagctg ggcttgcaga
     5701 agatatatga ttatgccaag atatacagca atggtcagaa cgctattcgt atgacggaag
     5761 gtcagccaat gggcagtttc tatggatata tctcagaggg tgtcgatccg gaaagcggca
     5821 atatcaaata cagggatctg gatggcaacg gaatcataac acctgatgac aggaccttta
     5881 ttggacaagc ttctccaaag tttatttacg gtatgaccaa caacttgtct ttcatgggat
     5941 ttgatctgag tatttttatt cagggtgtcc agggcaataa gatattcaat gccaccaaga
     6001 tggacctgga agggatgttt gatagcaaga atcagtcgat cagggtgctg gacagatgga
     6061 aaaacccggg agatattacg tccttgccgc gagcgaatga ggcgggagac ggcagtctgg
     6121 ataatataca taattcgaca cgatttgtgg aagacggttc gttccttcga ctgaaggcgg
     6181 tgacgcttgc atacaacata ccgacaggat ggctcaagcg ggcaaatatc cgcaatcttt
     6241 ccatctatgc cacagctcaa aacctgctca cactgactaa atattcaggt tttgacccgg
     6301 aagtcaatgc ttacggggtc aatagcaggg gcacggagtt tggtgtggat tatggaacat
     6361 atcctcagac acgacagatc atatttggat tgaatcttgg tctttgatgt ctgacaatga
     6421 acaaccttaa aaaatttaaa atgatgaaaa agaaaatata tttagcaatt gcgatgttct
     6481 cgggactttt tctgacttcc tgcgacagca tattggataa ggaaccgctt tctgagctga
     6541 ttgacaaaac gacagatacc gataagccat ttacagccgc ggatgccgag gcggccatgg
     6601 cagcagctta cgcggggttg aaaacttcac aggcggagct gatgatgctt gattattatg
     6661 tcaatggaga tgcacagtca gacaattcgc atgccggagc ggacaatccg gataactttc
     6721 agatcgacga ctataagatt tcttccacca acaacaatgt ttctagagac tggagatatt
     6781 tttatggcct tgtggcactg tgcaataccg tcattgaaaa ggcgccgact gccgcagacc
     6841 ttactccggc aaggcgcgat gaaatgattg gcgaggcatc tttcttgaga ggatggttat
     6901 attttggtat ggtgcagttg tggggagggg tgccaatagt gaccaagcct gttcctgcga
     6961 tttcggccga aaattttgag gaagtatata aaatattata cccggatcga aaatctctgg
     7021 aagaggtgta tgctcagatt attgaagacc tggtgcgcgc cgtcaatttt gcgccgactg
     7081 cctatggccc caacaagatg cgggggacca agtatgcggc ccatgctgtt cttgccaagg
     7141 tatatgccac catcaatccg catgattggg ctaaagtcaa tgaacattgc gatgctgtca
     7201 ttgccggagg attcagcctt ttgccggttt atgatcacct gtgggatgga aatcacgaaa
     7261 acagtgccga ggccatattt gaaatgaact gtaccgattg gaatacaggt ggcaactggg
     7321 gatcttccat gtttgtcggt acggactgga aaaaattcaa taccccaacg cataacctgg
     7381 tggatgcttt tgatgccgca ggagataacg ttcggaaagc gagtagcatc aagtttcaga
     7441 aggtcacctc ctggaccgat gcttactggg atactaacaa ctacccgttc tgttttaaga
     7501 tgaggaatac ggcgggggga caaaatcaga tcttcctgcg cctggcggat atatatcttt
     7561 tgaaggcaga ggcgttgacc gaattgggac aattggataa tgtttccggt gctcaatatt
     7621 atgtcaatct ggtccgaagt agggtcagtc tgacaccaac aacagcgaca acagccgatg
     7681 cactacgtac tgccatagcc aacgagagaa ggcttgaact ggcttttgaa ggccatcggt
     7741 ggtatgacct gaaacgtacc ggtaaggcag tagagacgat gagtgcactt ggatataata
     7801 tcaatgagaa taaattattg tggccaattc cacagggaga acgggataag aacatcaacc
     7861 tgacccagaa tccgggttat tgacagatac cttcccggcc cggaattgtg aagtggtaga
     7921 ttatggtgag caactatttt cggtgctaag ataacattgc ttttgggtgg gcgatattta
     7981 tcggaagaat gaatatcgcc acgccccttt atcaataaat agacttgatg gatatgagaa
     8041 tcgtaagaca gcctttgtat gtacttttga tgctggcatt gttttccttt aaatgttcga
     8101 aggatgatca accagacacg cctgtagatg tgccggtctc cgatgtccaa ttttatcaaa
     8161 catcagccga tggaagctcc ttactccaaa agcagaagca gacactgaag ttttcgaaca
     8221 aaacgaacca atatccaata cttaaggtgg acagcacaca gcagtttcag gagcttgatg
     8281 gttttggtta tagcctgacc gggggaagtg ccattgtttt gaatacgctt tctgcttcag
     8341 atcggaataa gatattaagg gagctttttt cgagagattc atcggccctg gccatttctt
     8401 gccttcggat aagcattggt gcatctgacc tggatccggc gcctttttcc tatgatgatg
     8461 taccggaagg ccagacagat atcaacttag aaaaattcag tattgaaccg gacaggacca
     8521 atcttattcc tgtcttgaag caaatcctgg aaatcaaccc tgaaataatg attatgggct
     8581 caccatggag cgctccccca tggatgaaga ctaataagaa tttcaagggg ggcagtctta
     8641 agaaagaata ttttccggtc tacgcccggt attttgtgaa atacattcag gccatgaagg
     8701 ccgaaggcat caatatccat gccattactg tccaaaacga acctcttcat ccgggcaaca
     8761 acccatcgat gctgatggaa gctcaagacc aaagagattt tgtacgggat tacctcggtc
     8821 cggcttttga aaatgcaggt atcacgacga agatcatcat ttacgatcac aattgcgaca
     8881 aaccggacta tccgatatct atcctgaatg acgccacagc aaaaaagtat atcgacggct
     8941 cggcttttca cctgtatgcc ggggaaatat cagccatgag taacgtacat gaggcacacc
     9001 cggacaggaa cctttacttt acagagcaat ggaccggtaa gaatgagtcg tttgcatcca
     9061 attttatgtg gcatacccag catgtcgtca ttggttcgtt gcgcaattgg tcaaggatgg
     9121 tactccagtg gaaccttgcc aacgatcccg gtttcggacc ccatactgac ggagggtgtt
     9181 ctgagtgcaa gggagcactg accatcggag ggaccatcac caggaatccg tcgtattata
     9241 tccttggaca gatttcggcc tgggtaccac cgggtagtgt ccgtgtcgca agtgataatc
     9301 tgcccggact tcaaagcgtg gcattcctta caccgaccgg aagaaaggtg ttgcttgttc
     9361 tgaacaccac acagactaac cagtcattca atatagaatt taaggaccgg agggtgacga
     9421 cattgctgaa ggcgcagtct gccggaacct atatttggta gtaataaaaa aataaggata
     9481 tgaataagat ggccaagaac ctaatcgtgt tgtgcatatt aacggtgact ttcccgctgg
     9541 tgacctcagc acagggcaat gttgcggtct taagtcctgc aacggaaatc ggcaaatcag
     9601 gtcgtgcctt tatttatacc acagcattca acacaaagct aaggctgacc aggtccggga
     9661 taagtgagtt caggcccaaa gggcagccgc tggagacaga aatctgtgtt tttgtcgatc
     9721 cgaacaaaaa atttcaggaa tatatagggg tgggtggggc catcacggat gcatctgcag
     9781 agacttttgc cgccttgcct tcggaaaggc aaaaagagtt gattacggca tattttgaca
     9841 gagaaaaagg catcggatat aatatcatca ggacgaatat caacagctgt gacttcagta
     9901 gtggcagcta tacctatgtg aaagacaagg atgttacact ggagtcattc gatataggac
     9961 acgatctgaa attcaagatt cctatgatca aggaagcaca gcggataatt ggcaacggaa
    10021 tgcgtttttt tgccagcccc tggtcacctc cggcctggat gaaggacaac aacagtatgt
    10081 tgcatggcgg aaagttgctt ccggaataca gagatagctg ggccaggtat tatgttcgct
    10141 ttatcaaaga ctatgagcag caaggaattc cggtttgggg tatttccata cagaatgaac
    10201 ctatggctac acaacgctgg gagagctgca tctatacggc tgaagaggaa cgagattttc
    10261 ttaaaaaaca tctgggcccg gtaatggaac aggaaaaact gggagacaag aaaatcattg
    10321 tttgggatca caaccgagat ctgatatacc agcgtgcgca gacttatatg tctgatccgg
    10381 aggccgcaag atttatctgg ggcatcggct tccactggta tgaagactgg agtggcggca
    10441 cacccatgta tgacaatcta caaagagtat atgaggcatt cccggaaaag aaactgttct
    10501 ttacagaagg ctgtgccgaa agttttcgcc aggatggtta taacaattgg gaacttggtg
    10561 aagaatacgg acgaagtatg attcgcgact tcaacaatgg catggtggga tttacagact
    10621 ggaatatttt acttgatgaa aatggcggcc ccaaccatgt aaagaatttt tgttttgccc
    10681 cggtccatgg cgatacaagg acagggcaac tcatatatac caatgcctat tattacattg
    10741 gccatttttc taaattcata gaaccgggag cccgccgcat cactgcatca tcgacacgca
    10801 gccccttgct gagcacggcc tttgagaatc ctgatggcac cctggttgtc attgtgatga
    10861 atcagggcga taaggatatg gattgcctgg tatggatcga tggggctgct gccgaagcaa
    10921 atattcgggg gagaagtatc aacacgattt tggttcctcc tgccagatga gccgggagaa
    10981 aagttgaaga atttgccggg agaccatgct tttattacaa cacaatcccc ccgtcgattc
    11041 taatcactgc cccattgata taagccgctt catcggatgc aaggaaggcg tatagggcgg
    11101 ccacctcctc cggttgcccg aggcgtttga cgggcacctt ggcttccata cccgccagga
    11161 catcttccgg catcaactga atcatttcgg tagcaataaa tccgggtgca acggcattga
    11221 ccgtaatgcc ctttcggccc agttccttgg acagagtctg ggtcatgccg atgagaccgg
    11281 ccttggtagc agcataattg gtctggccga agttgccaaa gagtccgacc accgacgatg
    11341 cattgatgat gcgaccgtaa ttgttttgtg ccatgatggg ggcgatggct cgtgtgcagt
    11401 gatatacgcc gttgagattg acgtcaatca cgcgctccca ttgctcgtcg gtcattttca
    11461 acagcgttga atccttggta atgcctgcat tgttgattag aatgtctatt ctgccataac
    11521 ggctgatgac atcttccgtc gcctttgcta tttgttcttt gtcggttgtg tccacgtgat
    11581 aataggcagc attgcttccc ttgtccttca atgcagtgat ggcttcttct ccttttttat
    11641 ccgcgacatc ccagatgatc acatgagcgc cttcatccgc aaaacgcagt gccgtggccc
    11701 tgccgatacc acttccgccg cctgttatga cagcaatttt attttccagt ctcttcatta
    11761 ggtaaaatta tgtttttaaa taaaattgat taacccggca aaaataaggc cgtatccatt
    11821 ggaataggaa tattatttaa actaaaacgc caaaaggtcg caccataaaa tataatcata
    11881 taatcctata tatcaaaaat ataagttaaa taattataaa aagtcgcacc aaaatataaa
    11941 caaataacaa tatgattatc cgaatgaatc cttcaacttt gtaggcgtta gacagcaatg
    12001 gtttatatga atacatctat agaatggatc tgaagcaggc gactatctta cttttggcag
    12061 tatatgctgc catcctactg tttttggtgt atcgtggtgc aaggaaaacc cgtacattga
    12121 aggattatgc cattggatcc ggttttccgg ctattgtcgt tgccttttcc cttgcagcca
    12181 gtgtttcgag tgcggcgaca tttatcatca atccggggtt cattgcgtat tacggatggt
    12241 cggcctttct ggccatggcc ctggtgttac cactgggact ttatttgtct ctgatctatc
    12301 tgagtaagag gtttcaacat catggcaact tcaccagtgc cttgactctg cctcaatggg
    12361 cgggtaatag attcaacagc aaggtgttgg agcgacttct tgccatattt tcgctgttga
    12421 tgattacttt cctggtgctt atctgtgtgg gtctggccaa gataatcagc aattcccttg
    12481 gagcggatga ggtctctgta atgatcggtg tcgttgtttt tgtatttgga tatatgatgt
    12541 ttggcggggc caacgctctg gtatatacta attttgttca ggcagtgctc atgctcatcg
    12601 tggccgtcat cctgatcggg tccggatatg gctactttga aggcggagta ggtgattatt
    12661 acgacaggct gaatacgatt gatcctatgc tgaccaaggc ctttaatcca accagcccat
    12721 tgttccgtga ttggtttgaa gtgattttct gcaatttcgt cgtaggaatt gctatagtat
    12781 gtcagccgca tatcatcacc aagtctttgt tgctgcggtc tgaaaaggac atcaacaagt
    12841 atctttttta ttctgtcgtc gtgctgatcg tcttcttttc cgttgtacta gccggcttct
    12901 atggcagaat gatgtttccg gatctcaaac ttgacggtag cacaatcaat ctggacagta
    12961 tcatgtcttt atatgttgtg cggcggtttt ctgcaggtgt gggtgtactt gtggtgtttg
    13021 gtttattggc ggccggaatg tctacacttg aatcgctggt acaatcgcta tcaatcacca
    13081 tcacacaaga tctggtccgg cctgcattgg gaaagaaagc ctcaaaggtc aatctcaagg
    13141 tggtcaacag atttgttatt gcttttctgg cattggtgac catattggta agttacaatc
    13201 agatggtcaa tcccaccctc agtgtgggca ttctggctca gaacggagtc tattccttct
    13261 tttcagccgc ttttattcct gttttgttcg gagtatttgt caaggatgct ccaaagtctt
    13321 ccgtgattgc cgcatctatt gtggcgattg ttgtgcattt tagtgtgtat taccttgggt
    13381 tgacaccata tacatcggga gcggtacgca acccggcagt ggcttccgcc ctggctattg
    13441 tagccgcatt tttaacaggg acgaccctgt tgttaattga ccgaaaaaag agaataatcg
    13501 aatgaataac gcaaaaataa tcgcctccgg ctggtacgcg ccatcccgaa tcgtcgataa
    13561 tcaatatttc aatgagatat tgggagagga tgtggacaca tggttgcgcg agaaagtgca
    13621 aatatatgaa agacgatggt gtccggatga ggaaagtgtg gctgacctcg ccgagcaagc
    13681 tatccgacag gctatagacc atgcgggcat ccggcccgat caggtggatt tacttatcat
    13741 tgcaacagat acaccggaat tcatttcgcc atctacggca tccgtcattc aagacaggtt
    13801 gcacctgacc aatgccggca ccttcgacct taatacagca tgtgcaggat ttgttactgc
    13861 gctggacgta ggcagcaaat acatacaggc ggataatcaa tataataatg tagtagtggc
    13921 gggtgtctat gccatgagca agtacctcaa tttgcaagac aaaaaaaccg ttaccctttt
    13981 t
//
LOCUS       DAOQMC010000034        13835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1628_length_13835_cov_4.713425, whole genome shotgun sequence.
ACCESSION   DAOQMC010000034 DAOQMC010000000
VERSION     DAOQMC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_13835_cov_4.713425"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1210
                     /gene="corA"
                     /locus_tag="PKV31_02515"
     CDS             101..1210
                     /gene="corA"
                     /locus_tag="PKV31_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNB91756.1"
                     /translation="MNRKLFKKSKAVKNIFRQDANRSASIKTGLAPGTLIFTGIQKVE
                     HPFIRVVLFNENNIEQYVISDGKVGNIRSGQLKTWIDCVGLHDVGLIEALGKKFNISR
                     MVLEDILNIGQMPKYINLDKGNFLTIQSFVQNRGTEELSKEQLSVYFEDNLAITFRED
                     ERNDFNPIYSRIVNPESRLVKNDIYYLVYSMLDLTVDNYLETAHYFESEIEKYEILIE
                     TRPDNDLKKEIFNFRRSFLYFFKMIAPLEEAISRFKADDEDLVNSVTKIYISDLLDHC
                     RRLRNLAETYNEMIYMLYDLLHTEINYRSNTIITTLTVISAIFIPLTFIVGVYGMNFE
                     YMPELTWNYGYYLIWAIMILFTISAIVYFRVKKWI"
     gene            complement(1244..2101)
                     /locus_tag="PKV31_02520"
     CDS             complement(1244..2101)
                     /locus_tag="PKV31_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91757.1"
                     /translation="MKYSGLTYLIAFVLAIAIVIYQYSVYGTWLSPYLLIPVILGVTG
                     YTLGPQIDWWFYKRFPPKLDDKVKRIFEINSEFYRNLPPEKKELFDTRVCLYIEDKDW
                     QYVSMDEEMPYDIRAIIAFEPVRLTINLENFLMRPFDRIAVYQNPFLSPFYPDRYHGS
                     ELYSEDGIVLFSLKHIIPAFRQPLEYFNVVLYEYARIMIATYKNLDFPKLDDHFWYDL
                     YKVANYGHREIMGAIGLPDVDALSVAIHHYFVYGQYFRTIYPELCIQLDMIFESDKNF
                     GQLALTNNL"
     gene            2334..2406
                     /locus_tag="PKV31_02525"
     tRNA            2334..2406
                     /locus_tag="PKV31_02525"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2367..2369,aa:Phe,seq:gaa)
     gene            complement(3251..4288)
                     /gene="holA"
                     /locus_tag="PKV31_02530"
     CDS             complement(3251..4288)
                     /gene="holA"
                     /locus_tag="PKV31_02530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNB91758.1"
                     /translation="MAEYSFEDIEKQINNRKLHPVYILQGEETYFTDLLTRKFQDELL
                     EEDEKAFNQEVYFGKETTVSTIIDSLSQFPMGSDYKLVLIRNAQELKDIDKLTSYVND
                     PFESSVLVLSFTGKSMDGRKKLPSIVKSKYGFFEAKTIYENQLPAWIKSEVKTKGKEI
                     SDQNATLIADYLGKDLQKISNELDKTVIAIGDAKEINQEHIENYIGINREFNVFELIN
                     SIFNKNPYKARLIVEYLTDNLNKQPLTLILNSLFNSFLKLYLLKVNNPKSDMDITAII
                     GTRSISAIKEYRTAMANFSLPDLDYAMYLFKIHDQLSKGIDSGNADHGELLKELVFKL
                     LHVKEVQVCPK"
     gene            4515..5801
                     /locus_tag="PKV31_02535"
     CDS             4515..5801
                     /locus_tag="PKV31_02535"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB91759.1"
                     /translation="MIANLPETELKRVVIIGAGFGGLKLARKMIGSGYQVVLIDRENY
                     HQFQPLLYQVATAGLEPSSIAFPLRKVFQKETNFHIRIDEVRFINLDEKFILLKNIGR
                     VNFDKLILAYGADTNFFGNENISKYSIPMKSVPEALALRNRILSDYERALSITDYDLR
                     QELMDIVIVGGGPTGVEIAGALADMRQFILPKDYNELDKQEVDIYLVDSGNKLLAAFD
                     EKSSLHAERDLESLGIHILKENRVIDYDGLNVAMSDGKIIRSRKLIWAAGITVKKIDG
                     LPETIYSRSGRINVDGYNQVIGLEDVYAIGDCAIMVTDDKPNGDPQVAQVAMQQAVTL
                     AKNLKAMIKGTTLKTFHYHDKGIMATIGRKKAVAEVFGVKFGGFFAWLTWLFVHLMSI
                     LGTKNKLMIFINWTVAYFTRDQSLRLIIKAKENKSN"
     gene            complement(5817..7760)
                     /locus_tag="PKV31_02540"
     CDS             complement(5817..7760)
                     /locus_tag="PKV31_02540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB91760.1"
                     /translation="MQLSIIIVNYNVRFFLEQCLSSIQKAGRTLDMEVFVVDNNSPDD
                     SVKMVRERFPMVNLIENKENFGFSAANNQAILQAKGKYILLLNPDTILSEDTLRICLD
                     FMETHDDAGAVCVKMIDGSGQYLPESKRGKPTLSASFFKMTGIYKLFPKSGFFNAYYS
                     GDISPNQIASIEVMTGAFMFLRQEVVRMIGLLDDTFFMYGEDIDYSYRILEAGYKNYY
                     LPTTTIIHFKGESTRKSTFNYTRTFYNAMIIFVKKHYKKQGRFYILFLQAAVIFKAIS
                     SYFLERILKILPLLIDFGLVYKALIGLKKWWGEWYFSNPDHINRQFDIVNAPLYAGIW
                     VLSLLAAGYYRARGAWTELLKGITGGTGVILIIYALLGQALRNSRSIIIWGALTTTLI
                     VIITKWLLNLLKYKRFGFKAERQIKYAIISGHSESERIRSLMSKNNRTYLFAGRICPD
                     DSIEEGTIGNLKHIEEIVDAYALSEVVFSQKDVAANKMMESMAQLTNEVDFRVAPDDT
                     LSIISSGDKNKQGELLTVGLNLNICTGGNKLKKRILDFTIAILLFMISPVIVLFQKKK
                     DNFFKNIFSVFYGQKTWVGYIEPIDSRILPTIKPGVLPCSGELTENSDADTVEKENLY
                     YARNYTWWKDLEVILKNLSNLSE"
     gene            complement(7840..9177)
                     /locus_tag="PKV31_02545"
     CDS             complement(7840..9177)
                     /locus_tag="PKV31_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB91761.1"
                     /translation="MEQLKGKQSVGAGFVALGLIFMFSGLIFGTIGAIQYVIPGLFKP
                     GLGFDKVRPLHVSSVVFWILMGACGSVLHYISESQGRLWSERLAKWQRFLFATSMIAI
                     LVSYVMGMFGGREYWEFNPLLAIPIIAAWLLFIINVFKSIKSFRHQPVYVWMWLTGAA
                     FFLFTYIESYLWLIPAFRSGIIHDMTIQWKSYGSMVGGWNMLIYGTSIFLMDKISGTQ
                     RYSYSGIAFSIYFLGLFNLMFNWGHHIYTLPGTKIIQYISYLVSMTELLLLGRIIYYW
                     RSSVSTAMRHYHILPYRFMFAADIWIFLTLILAIAMSIPAINAYTHGTHITVGHTMGA
                     TIGINTFLLLAFAADIFDMHIAKPSRTISISLYIAVGALFIFWISLVVAGIHKAYWQM
                     HTPEEAYSIMMYSLRPYFIIFMISGIILASALTVIIVSLLKRYFKMVFDFLLK"
     gene            complement(9158..9556)
                     /locus_tag="PKV31_02550"
     CDS             complement(9158..9556)
                     /locus_tag="PKV31_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB91762.1"
                     /translation="MADKIKVGILAVLSILFFCYTAYLYNRNYDPNAHSGLMSDKGKQ
                     IWQDKNCTACHQIYGLGGYLGPDLTNVYSHRPKDFIISRIQYGTDIMPAYQLSETQIE
                     EILAYLKSLDESGIASPAKLKLNIDGTIER"
     gene            9970..11949
                     /gene="ric"
                     /locus_tag="PKV31_02555"
     CDS             9970..11949
                     /gene="ric"
                     /locus_tag="PKV31_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNB91763.1"
                     /translation="MSATNETVINVQEIEPRLRHLTIFQAFDSLEEGESLVIHNNHDP
                     KPVYYQLARLRGEVFDWEYLEAGPEWWDVKVTKRPDADTYFENQTYEEIVIDVPSLEP
                     RVKHATIFQTFNQLRPGQSFIIHNNHDPRPVYYQLMDLRGNIFLWEYLQQGPEWWDIR
                     VTRTVPMIPMEEGRMIINVPAIDPVHKHGTIFHVFENMNPGESFIIHNDHDPKPLFYQ
                     LKDRHGDSFTWDYIMQGPHWFDILITIKEPSITADKEEVIIDIPSIEPAKKHATIFRM
                     FEELEEGKSFIIHNNHDPKPVYYQMLEMFGDIFDWEYLRQGPQWWDIRVTRRKSVTQP
                     NQRQPIDNDDAIIVDVPSLEPRLKHVTIFETFDKLEPGESLIIHNDHDPKPVYYQLQA
                     ERGDVFTWEYLQQGPQWWDIRVTRKGTETNETIGEMVAKDLRKADIFKKYGIDFCCNG
                     KKTVRQACADKGIDPVVVERELQQPTQESAYGHQLNYNEWTPDFLADYIINTHHNYTR
                     KSLPEIRNFMLKVAQVHGENHPELAEMRKLILRVHDEMMDHLLDEENDLFPTIKELVK
                     AKNNDGNCSVHSGQTFEAMIAIAEEEHDIVGRSMEKVRHLSNDYTIPSDACTSYKLLY
                     KMLEEFENDLFIHIHLENNILFPKAIEIERSIVSH"
     gene            12157..12975
                     /locus_tag="PKV31_02560"
     CDS             12157..12975
                     /locus_tag="PKV31_02560"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB91764.1"
                     /translation="MLINEKTKIAALLKYHPDALETIVSIAPDFKKLRNPILRKLMAG
                     RTSIAMASKIGGVRPEDFFKALKPLGFEYDDTGGPVEEIFEESFMPDYLKNLDEDRLV
                     SFDVRELIARGDDPLRSIQDKVKSLSPGQVLLIINSFEPVPLIKLLERQGFKAYVKQV
                     DGDTYETYFYKEGESGSRISENDAAISTSGDWEEMLTQFENRIEEIDVRHLEAPMPMI
                     TILEALDSLPEGKALYVHHKRIPVFLLGELNERRFGYRIKEVSENMVYLLIFKE"
     gene            13081..>13835
                     /locus_tag="PKV31_02565"
     CDS             13081..>13835
                     /locus_tag="PKV31_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91765.1"
                     /translation="MLLFLRTDIANDHYFYPPTLAITHIMALGWGTMIILGASHQLLP
                     VLIEGKLYSNRLAIMTFGFTCLGIPLLVFGFYIFRTGWIMQLGALLVNLGVLLYVSNV
                     FLSAFKSEKRNVHAWFIITASLWLLATTTFGLLLVINFDHTIFPKESLAYLSVHAHLG
                     LVGWFLLLVIGVGSRLIPMFLISKYTNEKALWTIYGLVNAGLIVFILARMVNRSLEFL
                     AIIPILTGIVVFGHHCFKAYAVRIRKAVDEQMKA"
BASE COUNT         4256 a         2464 c         2882 g         4233 t
ORIGIN      
        1 ccaaattctc ctttgagtta tctgatcaat gagcaaacca atgaatttat tcccaaagag
       61 agggtggaat tggattattg agaattgata aatttgtctc atgaatagaa aactttttaa
      121 gaaatcgaaa gcagtaaaaa atatctttcg gcaggatgcc aaccgatccg cgtcaatcaa
      181 aaccggtttg gcccccggta ctttaatttt taccggaatc cagaaggttg agcacccatt
      241 cattcgggtt gtcttgttca atgaaaataa tatcgagcag tatgttatct ctgatggcaa
      301 ggttggaaat atacgtagcg gtcaactaaa gacctggatc gattgtgttg ggttgcatga
      361 tgttgggctt atagaggcat tggggaaaaa gttcaatatt agcaggatgg ttttagaaga
      421 tatcctcaat atagggcaaa tgccaaaata tatcaatctt gataaaggga atttcctaac
      481 catacaatca tttgttcaaa atagaggtac agaggaacta tcaaaggagc aattatcggt
      541 ttattttgaa gacaacttag cgatcacttt cagggaagat gaaagaaatg attttaatcc
      601 gatatattca agaattgtta acccggaaag caggcttgtg aagaatgata tttattattt
      661 ggtatatagc atgttagatc ttacggtgga taattatctt gaaacagctc attattttga
      721 gagtgaaatt gaaaagtatg aaatacttat tgaaacaaga cccgacaatg atttgaaaaa
      781 agaaatattc aatttcagga ggtcattttt atactttttt aagatgattg cacctttgga
      841 ggaggcaata agccgattta aggcggatga cgaggatttg gtcaattctg tgacaaaaat
      901 atacatatcg gacttgcttg atcattgcag gcggttgaga aaccttgctg aaacctacaa
      961 tgagatgata tacatgctat atgatttgtt gcatacggaa atcaattatc ggagcaatac
     1021 cataataaca acattgacag ttatatcggc gattttcatc cctttgacct ttattgttgg
     1081 ggtatatggg atgaattttg aatatatgcc tgagctgaca tggaattatg gatattattt
     1141 gatatgggca ataatgatac tttttacaat tagcgccatt gtttatttcc gtgtaaaaaa
     1201 atggatctaa caggtagaca ttttatgcgc aattaattga gggttacaaa ttgttagtta
     1261 aagctaattg tccgaaattt ttgtcggatt caaatatcat atccaattga atacataatt
     1321 ccggatatat cgtcctgaaa tattgaccat atacaaaata gtgatgaatc gccaccgaga
     1381 gggcgtccac atctgggagt ccgattgctc ccataatctc cctatgtcca taattggcta
     1441 ctttatatag atcataccaa aaatggtcgt ccagctttgg aaaatcaaga ttcttatacg
     1501 tagcaatcat gattcgtgca tattcataaa gcacgacatt gaaatattcc agaggttgtc
     1561 tgaaggccgg aatgatatgc ttaagagaaa acaatacaat gccgtcttcg gaatatagtt
     1621 cagagccatg atatctatca gggtagaatg ggctgagaaa tggattttga tacactgcaa
     1681 tcctatcaaa tggccgcatc aggaaattct caagattgat ggtcaggcga actggttcga
     1741 aagcgatgat agctctgata tcgtagggca tttcttcatc cattgacaca tattgccaat
     1801 ctttatcttc aatataaaga cataccctgg tatcaaataa ttcctttttt tcaggaggaa
     1861 gatttctgta gaattcactg tttatttcga agatacgttt taccttatca tccagtttgg
     1921 gaggaaaacg tttataaaac caccagtcta tctgagggcc gagagtgtac cccgtaacac
     1981 ctaaaatgac agggatcagc aaataaggtg aaagccatgt tccatataca ctgtactgat
     2041 agatgacaat tgcaattgca aggacaaagg cgatcaaata agtcaggcca gaatatttca
     2101 tatcaaacat taaatgaaac acaaaactaa tcaaacaaat gaaaaccata tacgccgaaa
     2161 aaagttggag aggtgaaaga gttttaaaat gtgttaaggc cggagtggat cttctttcag
     2221 gaatggatct gcaactatga ttactttgag aatgctatga agacattgaa aaaatatttt
     2281 tttatcgaat atattgtcaa attgaaagta agttctatat ttgcatccgc tttggtacct
     2341 tagctcagtt ggtagagcaa aggactgaaa atccttgtgt ccctggttcg attcctggag
     2401 gtaccacaaa agccctgatc agttgatcgg ggcttttttt ttgagtgaag tcataggttg
     2461 gggattgatt gtttatataa gattttggga aaatattttt tagttaataa ggtttggcta
     2521 aagttgtaga aggaaggagc aggtgctgga agtcgaaagg gaagtaggta gatgattaat
     2581 aatcgggact tcttacagga ctggattttc ataagccatg gccaataaaa atcaaccaat
     2641 atcggtcaaa atattttcat aagaattgtg aagtagcgtt gtgacgtaga ttcgggttat
     2701 taatcatcta cttacttgaa tttttgccaa acgaacaaca ttgagtaaga ccagtatggt
     2761 gcgccgaagt aggtagatga ttaataatcg ggacttcttg cagaaccgga ctttcataag
     2821 ccatggtcaa taaaaatcaa acaatatcgg tcaaaatatt ttcatacgaa ttgggaagta
     2881 gcgttgtgac gtagattcgg gctattaatc atctacttac ttgaattttt gccaaacgaa
     2941 caacattggg tgagaccact aaagtgtgcc gaagtaggta gatgattaat aatcgagact
     3001 tcttgcagaa ctgagctttt catattgcct cccggaaaga atatgttaat ttgggtttca
     3061 aaatatttct tgtttgtcag aaactattgg agaggcttaa tttgagacta ttaatcttct
     3121 acctacttaa aattttgtcc tttcttagaa ttttttctcg ggttgatgac aaaatctaca
     3181 aaaatcgcgt tatcgtttaa tttaactata cttcagataa caagagtaag ccggccaagg
     3241 aaatcagcca ttattttgga catacttgta cctccttgac atgtaataat ttaaaaacca
     3301 gctccttaag aagttctcca tgatcagcat tccccgaatc tattccttta gaaagttggt
     3361 catgaatctt aaacagatac atagcataat caagatctgg aaggctgaaa ttcgccattg
     3421 ctgtccggta ttcctttatg gcagatatac ttctggttcc tattattgca gtaatgtcca
     3481 tatctgactt gggattgttt acttttaata aatagagttt caggaagctg ttgaaaagtg
     3541 aattaaggat caatgtcaat ggttgcttgt tcaggttgtc agtgagatat tccactataa
     3601 gtctggcttt gtatgggttt ttattgaaaa tggaattgat aagctcgaag acattgaatt
     3661 cacgatttat gccgatatag ttctcaatgt gttcctggtt aatttctttg gcatcaccga
     3721 tagcgatgac ggtcttatcc aactcattcg atattttctg gagatcctta ccaagatagt
     3781 cagcgattaa tgtggcgttt tggtccgata tttccttgcc cttggttttt acttccgatt
     3841 ttatccatgc tggtagttga ttttcataaa ttgtttttgc ttcaaagaat ccgtattttg
     3901 atttgacgat tgaaggtaat ttttttcttc catccattga cttccctgtg aatgaaagta
     3961 ccaggacaga cgattcaaaa ggatcgttta cataggatgt taatttgtca atatctttaa
     4021 gttcctgggc attacggatg agaaccagct tgtagtcaga tcccattgga aattgactga
     4081 gactgtcaat gatggttgaa accgtagttt cttttccgaa atatacctct tgattgaatg
     4141 ctttctcgtc ttcctccaat aattcatcct ggaatttgcg cgtaagcaaa tcagtaaaat
     4201 aggtttcttc accctgaagg atatacacag ggtgcagttt ccggttgtta atctgctttt
     4261 caatatcttc aaaggaatat tcagccatcc aattcgcaat tattggacga aggtaatgat
     4321 attgtcgcga atgaaggttt tgttattttt cttttaacta tatttattgt tagatataca
     4381 atattattta aatataataa aatcaacaat aaagatattt taacttaaag taatagttta
     4441 ggtggtttca atacttgaga gtctcataca ccaatctttt tttagctttg acattttaaa
     4501 aataaatttt aggcatgatt gcaaatcttc cagaaaccga actaaagaga gtagtgatta
     4561 tcggcgccgg atttggtggc ttaaagctcg ctaggaagat gatcggaagt ggttatcagg
     4621 ttgtgctgat agacagggag aattaccatc agtttcaacc cctgttgtac caggtggcta
     4681 ctgctggtct ggagcccagt agcattgctt ttccattgcg gaaagtcttt cagaaagaaa
     4741 cgaacttcca tatcagaata gatgaagtta gatttataaa tctggatgag aaattcattt
     4801 tactgaaaaa tatcggtagg gttaattttg acaaactgat tcttgcctat ggtgcagata
     4861 ctaatttctt tggaaatgag aatattagta aatattcaat accgatgaaa tcagtacctg
     4921 aggcacttgc tttgcgtaat agaatcctga gtgactacga gagagcactt tccatcaccg
     4981 actatgacct caggcaggaa ttgatggata ttgtaatagt cggtggcggc ccgaccggcg
     5041 tggagattgc aggcgcattg gccgatatgc gtcaatttat ccttccaaag gattacaatg
     5101 aattggataa gcaggaagtg gatatttacc tcgttgattc aggaaataaa ttgctggccg
     5161 cttttgatga aaaatccagc cttcatgcag agagggatct tgaaagcctg ggaattcaca
     5221 tacttaagga aaatcgtgtg atcgattatg atggattgaa tgttgccatg tctgatggaa
     5281 agataattcg ttccagaaaa ttaatatggg cagcaggcat tacagtaaag aaaatcgatg
     5341 gattgccgga aactatttat agccgtagtg gcaggatcaa tgtggatgga tataaccagg
     5401 taattggttt ggaagatgtc tacgctattg gtgactgtgc tattatggtg acagatgata
     5461 agcccaatgg ggatccgcag gtagctcagg ttgccatgca acaggcagta accctggcca
     5521 aaaatcttaa agctatgatc aaagggacaa ctttaaaaac attccattat catgacaaag
     5581 gtatcatggc aactatcgga cggaaaaagg cggtagctga ggtctttggg gtcaaattcg
     5641 gcggattctt tgcctggctg acttggcttt ttgtccacct gatgtcaatt ctcggcacta
     5701 agaataagct tatgatcttt ataaactgga cagttgcata ttttacaaga gatcaatctc
     5761 tgagacttat tataaaagcc aaggaaaata aatcgaattg aggcagtcgg aattaattat
     5821 tctgacaaat tggacaagtt tttcaggatt acttcgagat ctttccacca ggtataattt
     5881 cttgcatagt aaaggttttc cttttcaaca gtgtctgcgt cagaattttc tgttaattca
     5941 ccggaacagg gtaggacacc aggcttgatc gtcggtagaa ttctggaatc aattggctct
     6001 atatatccga cccatgtttt ttgcccataa aacactgaga atatgttctt aaagaaattg
     6061 tcttttttct tttgaaataa aactataacc ggactaatca taaaaagcaa tatagctatt
     6121 gtgaagtcca aaatcctctt tttcaatttg ttgcctcctg tgcagatgtt taagttaaga
     6181 ccaactgtaa gtaactctcc ttgcttgttt ttgtctccac tactgatgat gctaagtgta
     6241 tcatcgggtg cgaccctgaa atccacctca ttggtcagtt gagccatact ttccatcatc
     6301 ttattagccg ctacatcctt ttgtgagaaa acgacttcac tgagtgcata agcatccact
     6361 atttcttcaa tatgcttcag gttgccgatt gtgccttctt ctatggagtc gtcagggcaa
     6421 atccttcctg cgaaaagata agttcggtta tttttggaca tcagggaacg aattctttct
     6481 gactcagagt ggcctgaaat aatggcatat ttaatctgcc tttctgcctt aaaaccgaat
     6541 cttttatatt tcaaaaggtt caaaagccat ttggtgatta tgactatcaa agtagtcgtc
     6601 aatgcccccc agatgatgat agacctggag tttcgcagcg cctgacccag cagcgcataa
     6661 ataataagaa ttacacctgt gcctcctgtg atacctttca agagctccgt ccaggcacct
     6721 ctagcccgat aataaccggc tgcaagaagg ctcaataccc aaatgccggc gtacaacggc
     6781 gcgtttacaa tatcaaattg gcgatttata tgatccggat tggaaaaata ccattcaccc
     6841 caccattttt tcagtccgat caatgcttta tataccaatc cgaaatctat gagcaatggc
     6901 agaattttga gaattcgctc tagaaaatac gatgaaattg ctttgaatat gacagcagcc
     6961 tgaaggaata atatataaaa cctaccttgt ttcttgtaat gctttttgac gaagatgatc
     7021 atcgcattgt agaaagtgcg ggtgtagtta aacgtggatt tcctggtgga ttcacccttg
     7081 aaatgaatga tggtcgtggt gggtaaataa taatttttat aaccggcttc caaaatcctg
     7141 tatgaatagt cgatatcttc gccatacata aagaatgtat cgtctaacaa acctatcatt
     7201 cttactactt cttgccttaa aaacataaag gctcctgtca tcacttcaat ggaggcaatt
     7261 tgatttggtg agatgtctcc tgaatagtat gcattgaaaa atccgctttt tggaaaaagc
     7321 ttataaatac cggtcatttt aaagaaggat gctgataggg tcggtttgcc tctttttgat
     7381 tcggggagat actgtccact tccatcgatc attttaacgc atactgctcc ggcatcatca
     7441 tgagtttcca tgaagtcgag acaaatcctg agtgtatctt cagaaagaat ggtgtcaggg
     7501 ttgagcaaaa ggatgtattt tccttttgct tggaggattg cctgattgtt agcagcggag
     7561 aaaccgaaat tttctttgtt ctcaatgaga tttaccattg ggaatcgctc tcttaccatt
     7621 ttgacagaat cgtccggaga gttgttgtcc actacaaaaa cctccatgtc cagagtacgt
     7681 cctgcctttt gtatggagga caaacattgt tccagaaaga accggacatt gtaatttact
     7741 atgatgatgg acaattgcat acagggtgca aattagtcaa aaccaaacaa tcattgccaa
     7801 ataatgaatg cttggtttat ttctttggtg agaatttatt tatttcaaca gaaagtcgaa
     7861 aaccatttta aagtatcttt tcaacaagga gacaatgatg accgttaaag cggaagcgag
     7921 tatgataccg gatatcataa aaatgatgaa atatggtcgt agggaataca tcatgatact
     7981 gtaagcttct tcaggcgtat gcatctgcca gtatgcctta tgtattccgg ctacaacaag
     8041 gctgatccag aatatgaata atgcacccac tgctatataa aggctgatgc ttatggtgcg
     8101 ggaaggcttg gcaatgtgca tgtcaaaaat atctgctgca aaggctagta aaagaaaagt
     8161 gttgatgcca atggtggccc ccattgtatg tcctacagtt atatgtgtgc catgtgtgta
     8221 ggcattaatt gcaggaatgg acatggcaat agccaaaatt aaggtcagaa atatccagat
     8281 atcagctgca aacataaagc gataggggag gatgtggtaa tgcctcatcg ctgtactgac
     8341 cgaagaccgc cagtaatata tgattctgcc cagtaataaa agctctgtca tgctgaccag
     8401 atagctgatg tattggataa tctttgttcc gggtaaggtg tagatatgat ggccccagtt
     8461 gaacatcaga ttgaagaggc ccagaaaata aatggaaaag gcaatgccgg agtaactata
     8521 acgttgtgtt ccggaaatct tatccatcag aaagatgctt gtcccataaa ttagcatgtt
     8581 ccacccgcca accatcgagc catatgattt ccattgtatg gtcatatcgt gaataatacc
     8641 ggaacgaaat gcgggaatca accaaagata agattcgata taggtaaata gaaaaaaagc
     8701 tgctccggtt agccacatcc atacatatac aggttgatgt ctgaatgatt tgatcgactt
     8761 aaagacattg atgatgaata acagccaggc agcaataata ggaatcgcca gaagaggatt
     8821 gaattcccag tattctctgc ctccaaacat acccataaca tatgaaacca ggatggcaat
     8881 catagaggtg gcaaaaagga agcgttgcca tttagccaac ctttcagacc ataatctgcc
     8941 ctgtgactct gaaatgtagt gtagtacaga accacatgct cccatcaaaa tccagaatac
     9001 cacagatgaa acatgtaaag gcctgacttt gtcaaacccc aaaccgggtt tgaataatcc
     9061 gggaattaca tactggatgg ctcctatggt gccaaaaatc agtcccgaaa acatgaagat
     9121 tagacccaac gccacaaacc ctgcacctac agattgttta cctttcaatt gttccatcaa
     9181 tattcagttt taacttagca ggagaagcaa tcccactttc atccagactt ttaagataag
     9241 ccaatatttc ttcgatttgc gtctcactaa gctggtaagc gggcattata tctgttccat
     9301 actgaattct tgagatgata aagtcttttg ggcgatgaga gtatacattc gttagatcag
     9361 gtccaaggta tcctccaagt ccatatattt gatgacaggc agtacaattc ttgtcctgcc
     9421 atatttgctt gcctttatca gacatcaatc cggagtgtgc attcggatcg tagttcctgt
     9481 tgtacaagta ggcagtataa caaaagaata aaatgcttaa tactgctaaa atccccactt
     9541 ttattttgtc agccatctat gtgtgaattt tccaggcgtg aatataaggc aaatttcaat
     9601 tcaagttggg taatcaaaac ttcttaatga gatttttaaa agattattta attaaatata
     9661 ttttataaat tacagaaaaa cagtttttca ttattattat attaaagtaa tactttaata
     9721 ttaacctcct gagttcaaag cgctatttta cttttttcgt ttgtgtctta atgctgaaaa
     9781 atattacctg gcatttctta gcttgttttt ctttctactt tatctatata ggatcactcc
     9841 gtccgttttt ttcaggaaaa ttttataatg tagatttata ttatagaata aaagtactta
     9901 aatactttta tttgtaaaat tgaattatat ttgcattgat tttttaagct attaactgta
     9961 tacataatta tgtctgcaac caatgaaact gtaataaatg ttcaggaaat tgaaccaaga
    10021 ttgagacacc ttaccatctt ccaggctttt gatagtcttg aggaaggaga aagtctcgta
    10081 attcataata accatgatcc taaaccggtg tattaccaat tggctcggtt gagaggagaa
    10141 gttttcgatt gggagtatct tgaggcaggc ccggagtggt gggatgttaa ggtgacaaaa
    10201 agacccgatg cagatactta cttcgaaaat caaacttacg aggagattgt aatcgatgtc
    10261 ccatctttgg aaccaagagt taagcatgct acaatttttc agactttcaa ccagttaaga
    10321 cccggacaat cctttataat tcataataat catgatccaa ggccggtcta ctaccaatta
    10381 atggatctac ggggcaacat ctttttgtgg gagtatttac agcaaggccc ggaatggtgg
    10441 gatattcgcg ttacccgtac agtgcctatg attccgatgg aggagggcag gatgattatc
    10501 aatgtgccag ccatcgatcc tgtacacaag cacggtacga tattccatgt ctttgagaac
    10561 atgaaccccg gtgaatcatt catcattcat aatgatcatg atcccaagcc tctgttttat
    10621 caattgaagg acaggcatgg agattctttt acctgggatt atatcatgca ggggcctcac
    10681 tggtttgata tcttgattac cattaaggaa ccgtccataa cagcggacaa ggaggaggtt
    10741 attatcgata ttccttcaat tgaacccgca aaaaagcatg ccactatctt ccgtatgttt
    10801 gaagaattgg aggaaggaaa atcattcatc attcataata atcatgatcc caagcctgta
    10861 tattaccaga tgctggagat gttcggtgat atatttgatt gggagtattt gcgtcaaggt
    10921 ccacagtggt gggatatccg tgtgacaagg agaaagtcgg taacacaacc aaatcaaagg
    10981 caacccatcg acaatgatga tgcaatcatt gtcgatgtac catccctcga gcctaggctg
    11041 aagcatgtaa ccatttttga aacttttgac aagttagaac caggtgaaag ccttattatt
    11101 cacaatgacc atgaccctaa gccggtttat tatcaattgc aggctgagcg tggcgatgtg
    11161 tttacctggg aatatctgca acaaggccca cagtggtggg atatccgcgt gacaagaaag
    11221 ggaactgaaa ccaatgaaac aatcggtgag atggttgcca aagacttgcg caaggctgat
    11281 attttcaaaa aatatgggat agatttctgt tgcaatggca agaagacagt tcgtcaagcc
    11341 tgcgctgata aaggtattga tcctgtagtc gtcgagcggg aactgcagca accaacccaa
    11401 gaatctgcat atggtcatca actgaactat aatgaatgga cccctgattt tcttgctgat
    11461 tatattatta atacacatca caattatacc agaaaatctc taccggaaat caggaatttt
    11521 atgctgaaag ttgcacaagt tcatggggag aaccatcccg aactggcgga aatgaggaaa
    11581 ttgatcctcc gtgtgcatga tgagatgatg gatcatttgc tggatgaaga aaatgacctt
    11641 ttccctacta taaaggaatt ggtcaaagcg aagaataatg atggtaattg tagtgtacat
    11701 tcgggacaaa catttgaagc gatgatcgcc attgctgaag aggaacatga catagtaggt
    11761 aggtcaatgg agaaagttag acatttgagc aatgattata ctattccgtc agatgcatgt
    11821 actagctata aactgttata caaaatgctg gaagaatttg agaatgatct gttcatacat
    11881 attcatctgg agaataacat cctttttcca aaagctatag aaatagagcg ttcaatagtc
    11941 agtcattaac gataaaacag ctaattcagg acatatcgaa tgaaatggag actctttgag
    12001 ctatccgggg ttgatgaatt ttatataaag atttttttaa gaatgtaacc ttgcagaggt
    12061 atttatgacg aagggcatta ttagaattta tgtatttgtc gacctttaat catcaatgtc
    12121 aagtccgaaa gttttaatgt tttcaaataa cataaaatgt taatcaacga aaaaacgaaa
    12181 atagcagcac ttttgaaata tcatcccgat gctttggaga ccatcgtttc cattgctcca
    12241 gatttcaaga agttacgtaa tccaattctg cgcaaattga tggcaggtcg taccagtatt
    12301 gctatggcct ctaagattgg cggtgtcagg cctgaggact ttttcaaggc gttgaagcca
    12361 cttggctttg agtatgatga caccggaggg ccggttgaag aaatatttga agaatcattc
    12421 atgcctgatt acctcaagaa ccttgatgaa gataggcttg tgagtttcga tgtgagggaa
    12481 ctaattgcca ggggcgatga tccgttgaga tcgattcagg ataaggttaa atccttatca
    12541 cccgggcaag tactcttgat tatcaatagc tttgaaccgg ttccgcttat caaattgtta
    12601 gaacgacagg gttttaaagc ttatgtaaag caggtcgacg gggatactta tgaaacctac
    12661 ttctacaagg aaggtgagtc aggaagcagg atatcagaaa acgatgcagc tatttcaacc
    12721 tcaggtgatt gggaggagat gttgacacaa tttgagaata gaattgaaga aatagatgtg
    12781 cgtcatctgg aggcacctat gcctatgatt actattctgg aggctttgga cagtcttccg
    12841 gagggcaagg cactgtatgt ccaccacaag cgcataccgg tcttcctttt aggcgaattg
    12901 aatgaacggc ggtttggata ccggatcaag gaggtgtccg aaaatatggt ttatttattg
    12961 atattcaagg agtaatagca gatgttcgga ccgggaattg taggattaca acaaacgacg
    13021 aattacagag tggttctacc attctatatt tatgcatcag tatcattttt agcgggtaca
    13081 ttgttattat ttttacgtac agatattgcc aacgatcact atttctatcc gccgactctg
    13141 gctattactc atattatggc tttgggatgg ggcacaatga tcatcttagg tgcttcacat
    13201 caactattgc ctgttcttat tgaagggaag ctttatagta accgattggc tattatgaca
    13261 tttggattca cctgccttgg tattccatta cttgtatttg gtttttatat cttccggacc
    13321 ggatggatta tgcaattagg tgcattattg gtcaatctgg gtgtattgtt gtatgtatct
    13381 aatgtcttcc tgagtgcttt caagagtgaa aagcgcaacg tccatgcatg gtttatcatt
    13441 acagcatctt tgtggctgtt ggcgacaaca actttcggcc ttttgcttgt catcaacttt
    13501 gatcatacga tatttccaaa ggaatccctt gcctatcttt cggtccatgc acatctggga
    13561 cttgtaggct ggttcttgtt gttggtgata ggagtgggct ccaggttaat ccctatgttt
    13621 ctcatttcaa aatataccaa tgaaaaggca ctttggacga tttatgggct ggtaaatgca
    13681 ggtttaattg tattcattct ggcacggatg gttaaccgtt ctttagagtt tcttgcgatt
    13741 ataccaattc tcacaggtat tgtcgtattc gggcaccatt gttttaaagc ttatgccgtt
    13801 cggatcagaa aggcggtcga tgaacagatg aaagc
//
LOCUS       DAOQMC010000035        13749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1648_length_13749_cov_4.076968, whole genome shotgun sequence.
ACCESSION   DAOQMC010000035 DAOQMC010000000
VERSION     DAOQMC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13749
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_13749_cov_4.076968"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2576)
                     /locus_tag="PKV31_02570"
     CDS             complement(<1..2576)
                     /locus_tag="PKV31_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNB91766.1"
                     /translation="MSQNDANQLLYITSYTDDKRGISYSYVLQTVSGIPVYNAVTSFY
                     VNKKGDVGTVKSNFINSDTYRRDGSRATAIDVEEAVRSAAVACGIVSPQTRIIETRDN
                     KTILDGGNISSAPIEPFLTWVVRDGALVLGYQLEIAIIGALDHYQVIVDSRDGTLLNK
                     INYKLSCKFEGPGQYANHSHDSRCLDNHQSNILASTATPAPPPSGEVSKYRVYELPIQ
                     APNFGSSKVVVSPFDTVASPYGWHDLDGKEGMDATNLQGNNVHAFNDNSGNYQPDFEV
                     DGGAELNFDFSFDQNAEPQNSKDAAAVNLFYMINKMHDFSYHYGFDEKAGNFQANNYG
                     KGGEGGDYVKAMSQFGNGEGDFKNNADFLTPEDGGSGQMRMFLWDNASDKFNIEAPAS
                     IKGGYPVGNPQFGSAITTDKIEAEVVWAQDKTGGTNYFCDEAKNADEMSGKIVAIDRG
                     SCDFSLKVYNAQKAGAVGAIIMNFEDRIVDMASGTNGNLVTIPSVFVSKVVGDKIKDE
                     LKAGNTVVVRFQLPDNSGPALLDASYDNGVIAHEFGHGISIRLTGGPFNSGCLNSAEQ
                     MGEGWSDFMALVTTKQPGDKGTDARGIGTYVKGQSPSGTGIRRFPYSTDMKICPNTFE
                     TVASVSGPHAIGEIWCDMIWDLYWAMIDQYGDDGLLYGGEGGNSKAIELVFQAMKIQP
                     CGVGFIDGRNAILQADSLLYGGANNCLIWEVFARRGLGFNADGGSANSIADNVADFTP
                     NLFCLDKVQLEKEVTREVDPGGEIKVSLKLSSYKSATAKNILVSDIVPEHTTWVAGSG
                     GEYSNGVVTFRLDSLESSKNHEFTYLLKVDEGYSSKLLYQEGFEEEFPFPDGAWYSNV
                     LTG"
     gene            2793..3542
                     /gene="rlmB"
                     /locus_tag="PKV31_02575"
     CDS             2793..3542
                     /gene="rlmB"
                     /locus_tag="PKV31_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNB91767.1"
                     /translation="MEKQNIPYIYGRQPVIEAIKSGQPIEKIFLLSGGEREFEREIRD
                     GIKGLNIDLAYVPREKLNRLVHGNHQGVVAYINDYSYVTLDKLLHQIKTKNEPAFLMM
                     LDQITDVRNFGAISRSALLGGCHGIIIPSHNSVSVTSDAIKASAGALTRIAVCKVNSL
                     PEAADHLAQQGIQLFASGMGNSVLLHQLPLDEACCIVMGSEKSGVSKALESRADKIFS
                     IPQNELLDSYNVSVATGIVVYQTILKRKYNL"
     gene            3590..4540
                     /locus_tag="PKV31_02580"
     CDS             3590..4540
                     /locus_tag="PKV31_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNB91768.1"
                     /translation="MRHINNRKKTSLSNKGRTFLLYATGIFFLITTTVSCSHRSTIKI
                     AQETAPSNALLWKIEGKDLAKPSYLFGTIHLIPQDKFLFNDVMNKAFSECDEIVFEID
                     MNIMNDFSQMFGLLSKIRMPDDMTIKDLLSEDDYKLFQDKFADKGLPAFLLETIKPLF
                     ISTLADGMDSPNQSMISYEIELANKANDQNKTIGGLETIDDQLAAIDSIPLKIQAQML
                     IDELKKEHGENEYERLVALYIQQDIDALQKAINQGMSKDDQTMMNFLLKDRNVKWIPK
                     MNAMMKERPTFFAVGAGHLGGEYGVIKLLKEAGYKVSAIP"
     gene            4774..5292
                     /locus_tag="PKV31_02585"
     CDS             4774..5292
                     /locus_tag="PKV31_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNB91769.1"
                     /translation="MALILPVNEISPQWGEDCFFAENATITGDVIMGNQCSVWYNTVI
                     RGDVHYIRMGHKVNVQDGAILHCTYLQAPLNIGNNVSIGHRAIVHGCTIDDNVLIGMG
                     AIVMDHAYIPSNTLIAAGAIVLENTRCEAGMVYAGIPARPVKKLEPEVFEHNIRRIAD
                     NYVMYSGWMKKN"
     gene            5361..5795
                     /locus_tag="PKV31_02590"
     CDS             5361..5795
                     /locus_tag="PKV31_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91770.1"
                     /translation="MKVFQIHSQFSFYAILAFLVLIGSGCEKNDINNNSLNINQKTII
                     NNGSVVSNDSEGISLWMKEVTEDSRCPEELDCFWPGNGAIAFVLTIGNHSYPFTLNTY
                     ISPKDTLIGKYNINLTDLIPWPKANQTIPQSEYKAEVFVSKK"
     gene            complement(5819..6346)
                     /locus_tag="PKV31_02595"
     CDS             complement(5819..6346)
                     /locus_tag="PKV31_02595"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB91771.1"
                     /translation="MDNDVLREKKVSESKTTMTELVMPNDTNPLGNLMGGNLMRWMDM
                     VASICAGKHCEAHVVTASVDHVSFTHPIRMGEVVTIEAQVTRAFNTSVEVHVQVYAVD
                     FKGQKPKRSNHAYFTFVALEDETLRPQAVPQVLPLTQEETTLYENAIKRRELRLVLSG
                     RMKPGQATELKTMFV"
     gene            6618..7331
                     /locus_tag="PKV31_02600"
     CDS             6618..7331
                     /locus_tag="PKV31_02600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB91772.1"
                     /translation="MILNQKTAIVTGASKGLGKAISAGLIAKRAIVYGIGRNLEALEE
                     LKQELGGEFYPVQLDISREKDVKEWVDSTFSQTTKPDILINNAGIGSFHKLEETDSDT
                     WRGMLDVNLNGMFYMTSAVAALMKSKPESSHIINIGSILGKVGRTDSSAYCTTKFGVQ
                     GFTQALIQELRFYNIKVTCFNPGSIETDFLVSSGVQPHANMLQPEDLAQTIIQILETP
                     DNMQIDELVVRPLNPKAPE"
     gene            complement(7919..8353)
                     /locus_tag="PKV31_02605"
     CDS             complement(7919..8353)
                     /locus_tag="PKV31_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HNB91773.1"
                     /translation="MPHLEESFLKEAVDENGLHISPVLDDDIKNYLIDIDGTICDDVP
                     NEEPERMVTVEPYYDALEMCNKWYDEGHIITFFTSRTEEHRVITEEWLKQYGFKYHGI
                     LFGKPRGGNYHWIDNHIVKATRFKGKFTDLVTEMKEIEVFRK"
     gene            8694..9653
                     /locus_tag="PKV31_02610"
     CDS             8694..9653
                     /locus_tag="PKV31_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB91774.1"
                     /translation="MYSSDASGVDALAQATKDLKKEIGKVIVGQESVVQAVLISLFSN
                     GHSLLVGVPGLAKTLLISTISEVLDLEFKRIQFTPDLMPSDITGTEILDDNRQFKFTK
                     GPLFANIILADEINRTPPKTQSALLEAMQERVVTVAGQRYSLPKPFFVLATQNPIEQE
                     GTYPLPEAQLDRFMFNIPLDYPEYQDEIEVVRSTTSDKVANLRHILNADQIIYFQQLV
                     RKVPISDNVLEYAVNLAVKTRPGTERATDEVNKYISWGAGPRASQYLVLGAKCHAAIR
                     GKYSPDIEDVRAIAEYVLRHRIVRNYKAEAEGINESAIIRGLL"
     gene            9666..10073
                     /locus_tag="PKV31_02615"
     CDS             9666..10073
                     /locus_tag="PKV31_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNB91775.1"
                     /translation="MKIAVFRTAHFNAAHRLHNPSWSDERNVQEFGVCNNPHYHGHNY
                     ELEVRLAGEVGPETGYLINLDKLKSIIEADVIQYLDHKNLNLEIDEFRTLNPTVENIC
                     YIIWSRLRKALDDRYELRVRLYETPRNYAEYPA"
     gene            complement(10367..11680)
                     /gene="ahcY"
                     /locus_tag="PKV31_02620"
     CDS             complement(10367..11680)
                     /gene="ahcY"
                     /locus_tag="PKV31_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNB91776.1"
                     /translation="MEVTDNKLAYKVKDITLADWGRKEIQLAEAEMPGLMSLREQYGT
                     KKPLKGARIAGCLHMTIQTAVLIETLVELGAEVSWSSCNIFSTQDHAAAAIAAAGIPV
                     FAWKGMNEQEFDWCIEQTLHAFSGGQPLNMILDDGGDLTNMVLDRYPELVPGIRGISE
                     ETTTGVHRLYERVENGTLPLPAININDSVTKSKFDNKYGCKESLVDAIRRATDLMMAG
                     KVAVVAGYGDVGKGSAASLRGAGCRVIVTEIDPICALQAAMDGYAVKTMAHAIPEANI
                     VVTATGNLNIVTAEHFKAMKDKTVVCNIGHFDNEIDMAWLNENYGKTKVEIKPQVDKY
                     TIDGKDVIVLAEGRLVNLGCATGHPSFVMSNSFTNQSLAQMELWANSDKYKNQVYMLP
                     KHLDEMVARLHLAKIGVELETLSKEQADYIGVKVEGPFKPDYYRY"
     gene            12256..>13749
                     /gene="glgP"
                     /locus_tag="PKV31_02625"
     CDS             12256..>13749
                     /gene="glgP"
                     /locus_tag="PKV31_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNB91777.1"
                     /translation="MNDKFSNFKVPYSPAKQFSTRVAYFSMEFALDQSLKTFSGGLGF
                     LAGSHMRSAYELKQNMIGIGILWKFGYYDQVRNSDNNMAVLWQQKSYNFLEDTGIRFQ
                     VDVSDHPVWVAVWYLPPERFESVPMFFLTTDVPENDYLSKTITNHLYDYNPEAKIAQS
                     IILGVGGAKLIDIINFMPEVYHLNEAHGLSAAFYLYNKYKSAEEVRKRMVFTTHTPVE
                     AGNEKHNFVDLEKMSFFQSIPVAEIRQITGITGNIFDHSLVALRLSKIANGVSAIHGD
                     VARRMWGGYEGIAPITSITNSQNKKYWADYKLEKAASENNVTALEARKKELKYRLFIN
                     LADQTGKWFDPNVLTIVWARRFAGYKRANLITKDLDRFKQVFCNPQRPIQVIWAGKPY
                     PKDNGAIEMFNELVRLSANYPNIAIMTGYEMRLSKLLKQGSDVWLNNPRIPMEASGTS
                     GMTAAMNASINLSTWDGWIPEFVENGKNGFVVPPVDYQNLTEQQQDDQ"
BASE COUNT         4143 a         3057 c         2664 g         3885 t
ORIGIN      
        1 cctgtcagaa cattactata ccaggcacca tccgggaatg ggaattcctc ttcaaaacct
       61 tcctgataca ataacttgga agaataacct tcatctacct tcaacaaata agtaaattca
      121 tgatttttgg aagattccag ggagtcaagt ctgaaagtaa caacgccatt gctatattca
      181 ccgcctgatc cggcaaccca tgtagtatgt tccggaacaa tatcacttac caggatattt
      241 ttagctgtag cagacttata tgaagacaac tttaaactaa cttttatctc tcctcccgga
      301 tcaacttccc tagttacttc tttttccaat tgtactttat ccagacagaa cagattaggc
      361 gtaaagtcgg ctacattgtc agcgatggaa tttgcagaac ctccatcggc attgaagccc
      421 aatccacgcc tcgcgaatac ttcccaaatc aagcaattat ttgcaccacc atacaacaga
      481 ctatcggctt gtaggatagc attacgtcca tcaatgaacc caacaccaca tggctggatc
      541 ttcatggcct gaaataccaa ctcaattgct ttactatttc cgccttcacc accgtaaagt
      601 aacccatcat ccccgtattg atcgatcata gcccaatata gatcccatat catatcgcac
      661 cagatttcac caatggcatg tggtccagaa acactggcca ctgtctcaaa agtatttggg
      721 cagatcttca tatctgtgct ataaggaaaa cgtctgattc cggtaccgga aggagactgg
      781 cccttcacat acgttcctat accccttgca tctgtccctt tatctcccgg ctgcttagtc
      841 gtaaccaagg ccatgaaatc actccaacct tcacccatct gctctgctga attaaggcat
      901 cctgaattga aggggccacc ggttagacga atagatatac catgaccaaa ttcgtgagct
      961 attacaccgt tgtcatagga agcatccagt aaggcaggtc cggaattatc cggaagttgg
     1021 aatcttacaa cgactgtatt tcctgctttc aattcatcct tgatcttatc tccaacaacc
     1081 ttggagacaa aaactgaagg tatagtaact aagtttccat tggttccact agccatgtcc
     1141 acaatacggt cctcaaaatt cataataatt gcgccaacag ctcccgcctt ttgagcattg
     1201 tacaccttca gtgagaaatc gcatgagcct ctatcgattg ctactatttt acctgacatt
     1261 tcatcagcat tcttcgcttc atcacaaaaa taatttgtcc cacctgtctt atcctgtgcc
     1321 caaaccactt cagcttcaat cttatccgtt gtaatagccg acccaaactg aggatttccc
     1381 accgggtatc cacccttgat acttgcaggt gcttcaatat taaatttatc tgaggcatta
     1441 tcccataaaa acatcctcat ctggccactt ccgccatctt ccggagtcaa aaaatcagca
     1501 ttgttcttaa aatctccttc tccatttcca aactgtgaca tagcctttac ataatcacct
     1561 ccttcacctc cttttccata attattagcc tggaaatttc ccgctttttc gtcgaaacca
     1621 taatggtagg agaaatcatg cattttatta atcatataaa acaagttgac tgctgcagca
     1681 tcctttgaat tctgaggttc tgcattttga tcgaaagaga agtcgaaatt taattctgcc
     1741 ccaccatcaa cctcgaaatc cggttgataa ttaccgctat tgtcattgaa tgcatggaca
     1801 ttattcccct gcagattagt ggcatccatg ccctcttttc catccagatc atgccatcca
     1861 tatggtgatg caaccgtgtc aaaagggctt actacgacct tggatgaacc aaagttggga
     1921 gcttgaatcg gaagttcgta aaccctatat ttgctgactt cccctgaggg aggcggtgca
     1981 ggagtggcag tacttgcaag tatatttgac tgatgattgt ccagacatcg tgaatcatgg
     2041 gaatgattgg catattgtcc agggccctca aacttgcagg atagcttata attaatctta
     2101 ttcaataatg ttccgtcacg gctatccacg atcacctgat aatgatccag tgcacctatt
     2161 attgctattt caagctgata tcctaaaacc aatgccccat ctctgacaac ccaggtcagg
     2221 aatggctcta ttggagctga tgaaatattt cctccgtcca gaatggtttt attatctctt
     2281 gtttcaatta tccgtgtttg gggactgaca ataccacaag ctactgcagc gctcctgact
     2341 gcctcttcca catcaattgc cgtagcccta cttccatccc ttcgataagt atcgctgttg
     2401 ataaagttgc tctttacagt acctacatca ccctttttgt tgacataaaa actagtaacc
     2461 gcattatata ccgggatacc tgataccgtc tgcaagacat aggaatagga tattcccctc
     2521 ttatcatcgg tataactggt aatataaagt aactggttgg catcattttg gctcaagcca
     2581 aactttgcag ggttttgtaa aatttcattt tttacctttg tgtcgatcaa aaggttctgg
     2641 ccatgaatat ttcggcccgg tccaaacata aagcatgcaa aaagacaaaa ggcaattaaa
     2701 tttcgataaa tcttaaagta caacattaga taagttaatt ataaattttg ccaaagatat
     2761 aatttattca tatattcatt ttcaaataaa atatggagaa acaaaatatt ccctatatat
     2821 atggcaggca accggtgatc gaagcaatta agtccggcca acctatagaa aaaatatttc
     2881 tactatccgg tggagaaaga gaattcgaac gggaaatcag ggatggtatc aaaggtctca
     2941 atattgattt ggcttatgtc ccaagggaaa agctcaacag actagtacat ggcaaccatc
     3001 aaggggtcgt tgcttatatc aatgactatt catatgtaac tctcgataag ctacttcatc
     3061 agatcaaaac taaaaacgaa ccggcatttc ttatgatgct ggaccaaatt accgatgtca
     3121 gaaattttgg ggcaatatca cggtctgcct tgttgggcgg ttgtcatggt atcatcatac
     3181 cgtcccataa tagtgttagt gttacttctg atgcaatcaa agcatcagca ggggccctga
     3241 cacgaattgc agtttgcaaa gtcaattcct tgccggaggc tgctgatcat cttgctcaac
     3301 aaggtattca actgtttgct tcgggaatgg gcaattcagt gttgcttcat caacttcctt
     3361 tagacgaagc atgttgtatt gtgatgggtt ccgaaaaatc cggagtgtcc aaagcgcttg
     3421 aaagcagagc tgacaagata ttcagtatac ctcagaacga gttgctcgac tcatacaatg
     3481 tcagtgttgc cacaggaatc gtcgtatatc aaaccatcct caagcgcaaa tacaaccttt
     3541 aaactaccat tcagcaatcc atcatttctt catttagtaa aaccggtacg tgagacatat
     3601 caataatcgt aaaaagactt ccctatccaa caaaggaaga acctttctgc tatatgccac
     3661 aggcattttt tttctgataa ccaccactgt gagttgttcc catagatcaa caataaaaat
     3721 agcacaagag accgcccctt ccaatgcact tctttggaaa atagaaggta aggacctggc
     3781 taaaccaagt tatcttttcg gcactataca cttgattcct caggataagt ttttattcaa
     3841 tgatgtgatg aacaaggctt tcagtgaatg tgatgaaatt gtctttgaaa tagacatgaa
     3901 tatcatgaat gatttttcac agatgtttgg cttactttct aagatcagaa tgccggatga
     3961 tatgactatc aaagatctgc tcagtgagga tgattacaaa ctttttcagg acaaatttgc
     4021 cgataagggt ttgccggctt ttttacttga aacaataaaa ccgctcttta tttcaacttt
     4081 agcagatggg atggacagtc ctaaccagtc aatgatatct tacgaaatag agcttgccaa
     4141 taaggccaat gatcaaaaca aaacaatagg cggactggaa acgatcgatg atcaactggc
     4201 cgcaatcgac agcatacctc tgaaaataca ggcacaaatg ctgatagatg aattgaaaaa
     4261 ggagcatggc gaaaatgaat atgaaagatt ggtggccctg tacatccaac aggatattga
     4321 tgctttacag aaagccatca accaggggat gagtaaggat gatcaaacaa tgatgaattt
     4381 tttgctcaaa gacagaaatg tcaagtggat acctaagatg aacgccatga tgaaagaaag
     4441 accaacattc ttcgctgtag gcgcaggtca cctcggcggt gaatatggcg taataaagct
     4501 tctcaaagaa gccggataca aggtctctgc tataccttga accatcaact gatagaaaag
     4561 cgctgagaat taatgaaaat gggaatactt tcagcgtatt tacattctta attatatacc
     4621 gaggtcagtt aataggcagt tgaagtatca tggaggcgcc tgtttttgaa aaaagcttag
     4681 cgtaggagat acttgcatta gacacaaact ttaataaagg acatatgtct ctaatgccct
     4741 atatttacaa ttataatata taatgaatct actatggctt tgatacttcc ggtaaatgaa
     4801 attagtccgc aatggggtga agattgcttc tttgcagaaa atgccacgat aacaggtgat
     4861 gtaatcatgg gcaatcaatg cagcgtatgg tacaacactg ttatccgggg cgatgtccat
     4921 tacatccgaa tgggtcacaa ggtcaatgtc caggatgggg ccatcctgca ttgcacctat
     4981 ctacaagccc cattaaatat cggaaacaat gtatccatcg ggcaccgagc catcgtccat
     5041 ggctgcacaa ttgatgataa tgtattgata ggtatgggcg ctatcgtaat ggatcacgca
     5101 tatataccgt ctaataccct gattgcagcc ggagcaattg tattggagaa tacccggtgc
     5161 gaagcaggaa tggtatacgc cggtattccg gcccgtcctg tcaagaagct tgaacctgag
     5221 gtatttgaac acaacatcag acgtattgcc gacaattatg tgatgtattc cggctggatg
     5281 aaaaaaaatt gaattaaacc accagttcac aaacgaacaa ctgcatttat gcgttaataa
     5341 atttaaatca gattctttca atgaaagtat tccaaattca tagtcagttt agcttttatg
     5401 ctattctggc atttcttgtt ctcatcggtt ccgggtgcga gaaaaatgat attaataaca
     5461 actccctgaa catcaaccaa aagaccatta tcaacaatgg ttccgtagta agtaatgatt
     5521 cagaaggaat tagcctttgg atgaaggaag ttaccgaaga ctcaagatgt ccggaagaac
     5581 tggattgctt ttggcccggt aatggcgcta ttgcctttgt tttgaccatc ggcaatcact
     5641 cctacccttt tacgctaaat acatatatta gccccaagga taccttgata ggcaaataca
     5701 atattaacct tacagatcta ataccttggc ctaaagcaaa ccaaacaatt ccacaatcgg
     5761 aatacaaagc ggaggttttc gtctctaaaa aataattaac cccgtacagg actttcattt
     5821 acacaaacat agtcttcagt tcagtagcct ggccaggctt cattcggcca ctgagcacca
     5881 atcggagttc ccttcttttg atagcatttt catagagagt agtttcctcc tgtgtcaatg
     5941 ggagcacttg cggaacagcc tgtggtctaa gagtctcatc ttccagggcg acaaaagtga
     6001 aataggcatg gttgcttctt ttgggtttct ggcctttaaa atcaacggca tacacctgta
     6061 catgcacttc aacggaagta ttaaacgctc tcgttacctg cgcttcaata gtcaccacct
     6121 ctcccatcct gatcgggtgc gtaaaagaaa catgatccac actcgcagtc accacatgag
     6181 cctcacaatg ctttcctgca cagatgctgg cgaccatgtc catccaacgc atcaggttac
     6241 cacccatgag attgccaagc gggtttgtat cgttgggcat gacgagttct gtcatagttg
     6301 tcttagactc gcttaccttc ttttctctca aaacatcatt atccatagta aaaattgaag
     6361 ccgcaaaatt actgttattt tgataaaata aatccgaatt acgaaatata attttgctcc
     6421 agtcaataaa attatctttc taccaaatca actttgcaat aggatctatt ataattaaga
     6481 taataaacta cttttcccaa tggcaattgg ataggctaaa aggttaaaaa atcgaaaatt
     6541 ccacccattt ctgtatcttc tcaatttgcg ggcatcatcc aaaatcttta tcttagtgga
     6601 aatttatagt ttgtaatatg atattgaacc aaaaaacagc aattgtaaca ggagcatcca
     6661 aaggcctagg gaaagcaatt tcagccggtt tgattgctaa gcgagccatc gtgtacggta
     6721 ttgggagaaa cctcgaggct ctggaggaac ttaagcaaga actgggtggt gaattttatc
     6781 cggtacaact agacatcagt cgcgaaaaag atgtaaagga gtgggttgat tcaacatttt
     6841 cacaaacaac aaagccggat atcctgatta ataatgcagg aattggatct tttcataaac
     6901 tcgaagaaac agatagcgac acctggcggg gcatgctgga tgtaaatctg aatgggatgt
     6961 tttatatgac ttcggccgta gctgctctta tgaagtccaa acccgaatca tcccatatca
     7021 tcaatatcgg atccattttg ggcaaggttg gccgtacgga cagttctgcc tattgcacga
     7081 cgaaatttgg tgttcagggt tttacacaag cactcattca agaattgagg ttttacaata
     7141 tcaaggtaac gtgtttcaat ccaggctcca tagaaactga tttccttgtt tcatccggtg
     7201 ttcagcctca tgccaatatg ttacaaccgg aagatttagc acaaactatc atccagatac
     7261 ttgaaactcc ggataatatg cagatcgacg aattggtcgt aaggccactg aatccaaaag
     7321 cgccggaata aatatcattt ttgaaataca ggtcgattgc accgatgatc acctcagctc
     7381 atgttgtaat tgcacaaatg gtattctgta ctgattgtag cttcacggag gagtccattc
     7441 aatttcgaga actacagatt tttcatgaat taatatcccc ggcaacccat aacattcggt
     7501 gccttttgat aatctcgtgt cagaatggag cttttcctta attcaaactg ttgcccggcc
     7561 atgttattac tcccgtgagt agtttgtacg ttgatcttta gtcagattga aaaaaaatgc
     7621 tcttgaaatg catatcgcaa aattcgcaaa gcattcaacc cctatgagta ctacaaagct
     7681 gtttttaaga aacatacaac taatatctca atgattatct gtaagaaaaa tttttagtat
     7741 ttaaaatagt catactttgg gcaatattcg ccgaatccgg actatttgac ataacgtaag
     7801 ttaacgcaat cgccatggac gtgtatattt ccactcaaat gcttactgaa aagagcggat
     7861 ggtaccctaa atgcttggtc agcacctaac caggccccaa ccatctaact ggtcttaact
     7921 actttctgaa gacttcgatc tccttcattt cggttacgag atcggtaaac tttcctttga
     7981 atcgtgtagc cttcacaata tgattgtcga tccaatggta attgccgcca cgtggctttc
     8041 caaaaagtat accatgatat ttgaagccgt attgtttcag ccattcttcc gtgatgacac
     8101 gatgttcttc agtccgggaa gtgaaaaaag tgatgatatg accttcatcg taccatttat
     8161 tacacatctc cagagcatca taatacggtt cgaccgtcac cattctttcc ggttcttcat
     8221 tgggcacatc atcacagatt gtgccatcga tatcaataag atagttctta atgtcatcat
     8281 caagaaccgg gctaatgtga aggccgtttt catcaactgc ttcctttaaa aaggactctt
     8341 caaggtgtgg catggataat atttaattca ttgttccaag cagcaaagat agatagaatg
     8401 attcaatgaa taatcaattt taatgaatat ttaaggaagc aattggaata tttagcaaat
     8461 aacaattata aatccaatat cctgatatga ataatctatt atgaatgccc aatttattaa
     8521 aggccgctgt tgccttctca ggcaaagaaa actgagaagg cggacaaaaa accatgaatc
     8581 aacaaaagca gctaaacaat tccaactttg tattgttttt tgattttccg gtactttaca
     8641 ggaaaattat gcgttatatt tggcgccatt atttattcat ttaaaatatc aagatgtatt
     8701 cttcagatgc aagcggtgtg gacgctttgg cacaggcgac gaaagacctg aaaaaagaaa
     8761 ttggaaaggt cattgtcgga caggaaagcg tagttcaggc agtattgatt tcattattct
     8821 cgaatgggca cagcctttta gttggtgtac ccggactggc caaaacgttg ttgatcagca
     8881 ctatctcaga agtactcgat ctggaattca aaagaataca gtttactccg gacctaatgc
     8941 cttccgacat cacaggtacg gaaatcctgg atgataaccg gcaatttaaa ttcactaaag
     9001 gtccgttatt tgctaatatc atcttagccg atgaaatcaa ccgtacaccg ccaaagacgc
     9061 agtcagccct gctggaagct atgcaggaaa gggttgtgac agttgccgga cagcgatact
     9121 cactacccaa gccgtttttt gtattagcta ctcagaaccc aatcgagcag gaaggtactt
     9181 atcctctacc agaagcccag ttagaccgat tcatgttcaa cattccgttg gattatccgg
     9241 aatatcagga tgaaattgag gtggtcagga gtaccacttc tgataaggtg gccaacttaa
     9301 ggcatatcct caatgccgat cagatcattt atttccagca acttgtacgc aaagtcccca
     9361 tctccgacaa tgtcctggag tatgccgtca atctcgccgt aaagaccaga cctggaacgg
     9421 agagggcgac agatgaagtc aataaataca tttcctgggg tgccgggccg cgggcatcgc
     9481 agtatctggt tctcggagcc aagtgccatg cagccatcag aggcaaatat tccccggata
     9541 tagaagatgt aagggcgatc gctgaatatg tcctacgcca ccgtatcgtg aggaattata
     9601 aagctgaagc agaaggcatc aatgaaagtg ccatcatcag agggctcctt taatccgaat
     9661 cttccatgaa aattgcagta tttcgaacag cacattttaa cgccgctcac aggctccaca
     9721 atccctcctg gagtgacgaa cgcaatgtcc aagagttcgg agtatgcaat aaccctcatt
     9781 atcacggaca caactacgaa cttgaagtca gattggccgg agaggtcggt cccgaaaccg
     9841 gttatctgat caaccttgat aagctgaaat ccatcatcga agccgatgtc atccaatatc
     9901 tcgaccataa aaacctcaac cttgaaatcg atgaatttcg tacattgaat cccactgttg
     9961 agaacatatg ttacatcatc tggtccagac ttcgcaaggc gctcgatgat agatacgaac
    10021 taagggtaag gttgtatgag acaccgcgga attatgcaga gtatccggcg tgattaacat
    10081 tagtccggtg tatggcatga ttgaattttg acaaggtcat acctttcaaa tttcatttat
    10141 tttatatgat aaagtgaaaa tggcttacta tgaatgatgc attatcgtgt tttcatcata
    10201 agtgaactta catttatccc ctatttctca tgtcaaggta atgttcaaaa tagtttctat
    10261 aaaattattc ccattgccaa ctgacatcca tcaaaaaagc cattcccgac agcaggaatg
    10321 gcatttgtct atttgaatat tggcaggtta gaagctgcca aatggatcaa tatctgtaat
    10381 aatccggctt aaaagggcct tctaccttaa cgccgatgta atcggcttgt tctttactca
    10441 gtgtttcaag ctcgacgcca atcttagcta aatgcagcct tgcaaccatt tcatcaagat
    10501 gcttcggtag catataaacc tggtttttgt acttatcgct attggcccaa agttccatct
    10561 gggcaaggct ttgattagta aatgaattgg acatcacaaa ggaagggtgg cctgttgcgc
    10621 atcccagatt gaccaggcga ccctcagcca aaacaattac atccttgcca tcgatggtat
    10681 acttatccac ctggggcttg atctcaacct tggtcttacc gtaattctca ttgagccatg
    10741 ccatgtcgat ctcattatcg aagtggccga tattgcagac aactgttttg tccttcatag
    10801 ccttgaagtg ctcggcagta acgatattca ggttaccggt agcagtcact acgatgttgg
    10861 cttccggaat ggcatgagcc atagtcttga ccgcataacc atccatggca gcttgcaggg
    10921 cacatatcgg gtcaatttca gtaacgatga cgcggcagcc tgcaccacgt agtgacgcgg
    10981 cagagccctt tcctacatca ccataccctg cgacaacagc gaccttaccg gccatcatca
    11041 agtcagttgc gcgacgtata gcatccacaa gggattcttt acatccatat ttgttgtcaa
    11101 acttggactt cgtgacagaa tcattgatat tgatagcagg tagtggcaga gtaccattct
    11161 caacccgctc ataaagcctg tgcacaccgg tagtggtttc ctcactgatt ccgcgaatgc
    11221 ccggaaccaa ttcaggatat ctgtccaata ccatattagt caggtcacca ccatcatcca
    11281 ggatcatgtt caacggctga ccaccagaaa atgcgtgtaa ggtttgctcg atacaccagt
    11341 caaattcttg ctcattcata cctttccagg caaagaccgg aataccagca gcggcaattg
    11401 ctgcagcggc atgatcctgc gtagagaaaa tattgcatga actccagctg acctcagcac
    11461 ccaactccac gagtgtctcg attagtacag ctgtctgtat agtcatatgc agacaacccg
    11521 cgatccgcgc ccctttcaag ggtttcttgg taccatactg ctccctgagt gacataagac
    11581 cgggcatttc agcctccgcc agttggattt cttttctccc ccagtctgcc agggtaatat
    11641 ctttgacttt gtaagccaat ttgttatccg taacttccat taaatattct gattttttaa
    11701 tattggccgc aaaggtacga ctttatagga aaatattcga accaatatat gccaatttta
    11761 taacaaggat taaacccttg agtattcgcc tctcagtaca agccggacaa aacattgaat
    11821 caattaaatc gaatacaacc ggatctcaaa gcagtttaaa aaaaagcatt taaagccatt
    11881 atataaacaa attaattata catatatgcc taattatcta tattttacaa tagaactgaa
    11941 actcaatcac agttgaataa ctgattgtaa tattaagttc aacacaccaa ttcctgtaca
    12001 taacctggta gaggtctcag cttattgctg aacaattctc ctgattttcc ccatcaaaat
    12061 atgtggctaa ataatattac ctgattcatt ccatgttttt ggttgttcgc tttaaatttt
    12121 caagacctgc tgtatattct taatctaccg gtttgccgat ggtatggcca aatacaatat
    12181 tcaacgttta ggatattatt tttagttata tatttattta ttctattttt gccctttact
    12241 atatgatttt aatcaatgaa cgacaagttt tcaaatttta aggttccgta ttctcctgct
    12301 aaacaatttt cgaccagggt tgcctatttc tccatggaat ttgcactgga ccaatcgctg
    12361 aagacgttta gcggcggctt gggtttcctg gccggatcac atatgcgcag cgcttacgaa
    12421 ttaaaacaaa acatgattgg aatcgggatt ctgtggaagt tcggttatta tgatcaggta
    12481 cgtaattcgg ataacaatat ggctgtactt tggcagcaga aatcctataa ttttctggaa
    12541 gataccggca ttcgcttcca ggtcgatgtc agtgatcatc ctgtctgggt tgctgtttgg
    12601 tatctgccac ctgaacgctt tgagagtgta cctatgtttt tcctgaccac agatgtaccg
    12661 gaaaacgatt atttatccaa aacaatcacc aatcacctct acgattacaa ccctgaagct
    12721 aagatcgccc aaagtatcat cttaggtgtc ggaggtgcca aactgattga tatcatcaat
    12781 ttcatgcctg aggtctatca cttgaatgaa gcacatggac tttcagctgc tttttatcta
    12841 tataacaaat acaaaagtgc tgaagaagtt cgtaagagaa tggtattcac cacccataca
    12901 ccggttgaag ctggaaatga aaagcacaac tttgttgatt tggaaaaaat gagttttttc
    12961 cagtctattc cggtggctga aatcaggcaa ataacaggta taaccggcaa tattttcgac
    13021 cattccttag tggcactccg gctttccaag atagccaatg gcgtttcagc catccacgga
    13081 gatgtagcac gaaggatgtg gggcggatat gaaggcatcg cgccaatcac cagcatcacc
    13141 aatagccaga acaaaaagta ttgggctgat tacaaactcg aaaaagcagc ttcagaaaac
    13201 aacgtgacgg ccctggaagc cagaaagaaa gaactgaaat atcgtctctt catcaatctg
    13261 gccgatcaga caggcaaatg gtttgaccca aatgtcctga ctatcgtatg ggcaaggcgt
    13321 tttgcgggat ataagcgtgc caatctgatt accaaagacc tggatcgatt caaacaggtt
    13381 ttttgtaacc cccaaaggcc tatccaggtt atttgggcgg gtaaaccata tccgaaggat
    13441 aacggggcca ttgaaatgtt taatgaactg gtccgtttaa gtgccaatta tcccaacatc
    13501 gccatcatga caggctatga aatgagattg tctaaattgc ttaaacaagg aagtgacgtt
    13561 tggctcaaca atccaaggat acctatggag gcttccggca ccagcggcat gacagcagca
    13621 atgaatgcta gtatcaacct ttcgacttgg gacggatgga ttccggaatt tgtcgaaaac
    13681 ggcaagaatg gttttgttgt tcctccggtc gattatcaga acctgactga acaacaacaa
    13741 gacgaccag
//
LOCUS       DAOQMC010000036        13593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1675_length_13593_cov_4.486261, whole genome shotgun sequence.
ACCESSION   DAOQMC010000036 DAOQMC010000000
VERSION     DAOQMC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1675_length_13593_cov_4.486261"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKV31_02630"
     CDS             <1..418
                     /locus_tag="PKV31_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB91778.1"
                     /translation="MNTGGKNDFGFGEVRMVKAVHSSSLPDGSYGGNPTGFLIHADGV
                     TLYFAGDTALTKDMELIPSFYHKPDLAILPVGGCFTMDYEDALIASKLLQCDRVIGCH
                     FNTFPPISIDTVVAEELFAKNGKELILPEINQVINL"
     gene            422..1210
                     /locus_tag="PKV31_02635"
     CDS             422..1210
                     /locus_tag="PKV31_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB91779.1"
                     /translation="MGKIVAIANQKGGVGKTTIAINLGACLALMEYKVLIVDADPQAN
                     ASSGTGVTKEMTNYTIYDCFINEVPAVDAIVHSNTPNLDILPSDINLVGAELELVSRF
                     KREYIMKDFLEPIRDKYDYIFIDCLPSLGIITVNSLTAADSVIIPVQAEIFSLEGLGK
                     LKNTISLVRKQLNPHLTIEGIVLTMYDKRLRLANLVIEEVQEHAKDRVFDTIIHRNSR
                     ISEAPSSHQPVIMYDAGSKGTINFINLAHEFLKYQGAASEQATV"
     gene            1229..2110
                     /locus_tag="PKV31_02640"
     CDS             1229..2110
                     /locus_tag="PKV31_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNB91780.1"
                     /translation="MKKKDLGKGIRALLNSMDQEEAEPTIEAVRELSDGVAMIRISEI
                     ETNPDQPRSQFNDQQLNELAQSIKNFGIIQPLTLRKTGRNRFQIIAGERRFRAAQIAG
                     LQEVPAYIRLANDQELFEMALVENIQREDLNPIEIAISYKRLMEETNMTQENLSERIG
                     KDRSTIANYVRLLKLLPDIQATVKDKRLSMGHARALAGVDDIALQLAFYKQAMSNAWS
                     VRQLENNIRDHFHGKEVAKPTSRAKNPELEHLKDRLKKKFGVKVQVEQNEKGQGYLKF
                     IFNSTNELNGILDVIDG"
     gene            2152..2772
                     /locus_tag="PKV31_02645"
     CDS             2152..2772
                     /locus_tag="PKV31_02645"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNB91781.1"
                     /translation="MADMLHPLVKAIVPIVLIVISVHTLFCQNTDSLFLASPKDSSHL
                     TKDTVVTNWEGVDLKTPKKALIRSAILPGLGQAYNHQYWKIPIVYAGFGALIYAGEFN
                     RKNYRLLKEVYRNMIDGVPTDYDRYSRQSVRLARDNYRKNMELSYIALAGVYGLNILD
                     AFVSAHLKTFDINENISMKIKPAVQHEYIGLYAVPTGGLTFSFVLH"
     gene            complement(2764..3084)
                     /locus_tag="PKV31_02650"
     CDS             complement(2764..3084)
                     /locus_tag="PKV31_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNB91782.1"
                     /translation="MTSIKNFLNELPNKVIPAALEGVNTILHFDVTGDNGGLYTLRIA
                     DGKIDVSEGLIGDAKSVIRTSDKVFSDLVNKETNPMMALMTGKLKVSNPGEIMKYAKL
                     IGLM"
     gene            complement(3137..4501)
                     /locus_tag="PKV31_02655"
     CDS             complement(3137..4501)
                     /locus_tag="PKV31_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB91783.1"
                     /translation="MKRTITDEEFRKKQPDPQIAGPINFRPDQFFQLDNGLKIILVEN
                     HKLPRVNVQLYIDRGVFADGDKAGLQELAGQLLSCGTVRDSKYEWDRKIDYYGGSIHT
                     TSIGGTASSLTRYFKQVFQLFADAILTPAFPLEEFNNLKRQLLSNLAAQKSEPESILG
                     NLIKRRIYPGRHPYSEVITEKTVRNITVDDCREWFLTNSSPDNSYLIFEGDITMEDIK
                     ILVGDNFSSWIPRSVTTLEWPEDMKRNGVNVSFVERPGAVQSLIGIGYSINYLPYNAD
                     TDAANLMNSVLGGYFSSRLNLNLRENKGYTYGIYSRLTNDESIGSFTTSVSVRNDVTA
                     DTILEIVKEMNTLRDVDISVEELQQVKNVISGNFSRSVENPMIIAKFAFARHKFNLPE
                     DYFKNQLIRINKVSVDDIRLMAQKYLLTDNMHIAVIGSREVLNSLTAFGQVTEFDFEG
                     NPIG"
     gene            complement(4510..5796)
                     /locus_tag="PKV31_02660"
     CDS             complement(4510..5796)
                     /locus_tag="PKV31_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB91784.1"
                     /translation="MSKNHLSIDFEEYDLDNGLHVILHKDNTSPLVSISVMYHVGSKN
                     ESPDRTGFAHFFEHLLFEGSENIARGQFFNIVQNAGGTLNANTSYDRTFYYEVFPSHQ
                     LETGLWLESERMLHATVDETGIETQRSVVKEERRQRYDNRPYGRIVEESMQRAYTVHP
                     YRWPVIGSMDHIDAASEEDYRNFYARYYVPQNAALCIAGDIDTDNAKGLILKYFGTIP
                     KGELPINRPEPNEPPMAHELRDIVYDNIQLPAVIHLYHIPAFAKGDYFAVKLLSDLLS
                     TGQSSRLNKVLVDEKRIAVQVGSFPLELEHPGVVLQFAIGNPDTDPEVLESAMDEEID
                     LILKEGLTDEDFEKLINQTETKLVNEQSTLLSIAEGLSTYYTYFGDTSMFNKQMDFYR
                     SVKKEDIRQAALKYFNKDNRVALFWLPEAHKIEKVE"
     gene            5955..7616
                     /locus_tag="PKV31_02665"
     CDS             5955..7616
                     /locus_tag="PKV31_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNB91785.1"
                     /translation="MAVFKTLDKMIMKGFIIPFIATFFIALFILTMQFLWVWVDDLVG
                     KGVGALIILELLFYLLLSFFPIAFPIAVLLSSVMLMGGYAERYELSSMTSSGISLMRV
                     MKPLIFLSFFIACISFGFSNYLWPVANLKYRSRLLDIKNQKPTLSIQKGVFNRDFTGY
                     VIKVTDKDKDGRAVKGIMVYDNQRQIDKLNLITAKEGEMYTEPKDNLFIMKMFNGHQY
                     QEMNPQGSNEGRYPFVRMNFKEYTKVFDMSQFDFNRTDENLYKSHQSMLSIRQLKVAV
                     DSINSRLDRAVDDMNSGLMSSINSKYATTGYSAFNDRRESVDHQYFPKRDTQSMEKLK
                     RERLGIIPIKPQRKNTPAQIDEDFHSSPANLITSRDLFVNGNFKPGKPVFDQLNARFR
                     LASVNYAVSLAKSAQSTADLQSSIYAAEYDSKVRHIFEMHVKFAYSVICIVFLFIGAP
                     MGAIVRKGGFGYPLLVAIIFYMTYQVLFTIMKKSAESQVIPPALAPWIPVMVMALIGF
                     ALTRRAMSDGSMNLEVFSKFFTKIFDKASESYYQFVEFFLSWVKK"
     gene            complement(7719..9416)
                     /locus_tag="PKV31_02670"
     CDS             complement(7719..9416)
                     /locus_tag="PKV31_02670"
                     /inference="COORDINATES: protein motif:HMM:NF020677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNB91786.1"
                     /translation="MNNHIRFLLLFAVICLSSSVTAQFKFSDKEVTKVVYSTSYNDRT
                     DPNQNPIWVFADSGNALITNRDQMNNTAAFPFEMTLIHSNISSYAQYAFLKEGSCIAM
                     LDSQSINKQNWELLSDTKDILGYRCKKARTSINSNTIEIYYTDEMKIKGGPTLLGTGL
                     GFVLEMVRNGNYRISAIGTSKERFEWPAFLKNVTPEYLEKIDYNDRIWQSRFITIPVF
                     NHQQICFTDGPFDNSNEVMRFANGTIVVKKVRFPKIGQHDQIFLDMTQRSNGDAYDRT
                     GSVFLIPVDKRVSFLDALQKGIGELPIFDNGNGKKYQGVVLTPQYTPLLELMRFFTPF
                     GIGHFRDRVTLKGKTWLDSITYRQEVSDYAGELNDKEVYIGAFIGNYDKGGHIISMNF
                     TIHKEEDMDMPTRKVLPLFNTLNVMEMAGQDYGTMFDSEKGLKVEFELKETMKNCKLN
                     FITTGHGGWGRGDEFVRKKNTITLNDKIIFELIPWREDCGTYRLYNPVSGNFSNGLSS
                     SDYSRSNWCPGTVTNPYIIDLGDLPVGKHVLTVKIPQGKPEGSSFSSWNVSGALIGE"
     gene            complement(9494..11569)
                     /locus_tag="PKV31_02675"
     CDS             complement(9494..11569)
                     /locus_tag="PKV31_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNB91787.1"
                     /translation="MRYKHFLLTLIAGTIAFSCSEPNNSKKNEHSQNTVSGYDPNFKV
                     SAQEFADLKVLRYQVPGFDQLPLQQKILAYYLSEAALSGRDIIYDQQSKNGLTIRKTL
                     EAIYGTYSGDKSTENWKNFEIYCGRFWFSNGNHHHYGNEKFFPEVSFEYFKEIAKASD
                     PNLFPMDSGEDLDTFLERIKPIIYDPNVEPKLVDLRPNIDNVKASSVNFYENVSLRDV
                     ELFYEKFDSKGNAPMWGLNSKLVKENGKVFEKVWKSGGMYGPAIDKIIYWLEKASGVA
                     ENDQQRKAINLLIDYYKTGDLHTFDNYCIAWVNDTASRTDAVNGFIEVYQDPLGRKAT
                     YESVVSIKDLEATKRIQAIAKQAQWFEDNSPLMPEHKKKNVKGIIAKAITVLMEAGAT
                     APAGPIGINLPNNEWIRKEHGSKSVSLSNIVHSYNVDGATSGMLDEFVVNDEVKARIK
                     EFGDITSDLHTDMHEVIGHASGQINPGVESTDKTLKNYASTLEEARADLVALYYMIDP
                     KLVEIGVMPTVDAGKAEYDSYMMNGLMTQLTRIQPGNNLEEAHMRNRQLVAKWAYENG
                     KASNVVEFVKKDGKTYVRINDYNKLRQLFGQLLREIQRIKSEGDYKAGHDLVENYGVK
                     VDPELHREVLERFKKLNIKPYKGFIQPRYVPVMKGDKIIDVKVEYPDNFFTQMMEYGK
                     KYSFLPVKN"
     gene            complement(11746..12948)
                     /locus_tag="PKV31_02680"
     CDS             complement(11746..12948)
                     /locus_tag="PKV31_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNB91788.1"
                     /translation="MDYFKEALFLHKQLKGKISVQTKFTIKNRDDLSLVYSPGVAAPC
                     LEIAEKPETVYDYTLKGNTVAIVSDGSAVLGLGNIGAAAAIPVMEGKAMLFKRFANIN
                     AFPICLDTQNTQEIIDTVKRIAPVFGGINLEDISAPRCLEIENALQDLGIPVFHDDQH
                     GTAVVTLAGLINASKLLDKPLSEMRVVINGAGAAGIAIARLLRCVEHTDNSVCQPVKE
                     IIICDTKGIIHRSRTDLTDVKKSLLAFSNSENMQGTVKDAIRNADVFIGVSSASLLDR
                     HDIATMNKDSIIFALANPIPEIMPDEAYAGGAAIVGTGRSDFPNQVNNVLGFPGIFRG
                     ALDVRAPRISPSMKMAAAYAIADCIDQPTKENIIPATLNDQVAYKVAEAVRKAAMKEW
                     NLKQIYGF"
     gene            complement(12960..>13593)
                     /locus_tag="PKV31_02685"
     CDS             complement(12960..>13593)
                     /locus_tag="PKV31_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB91789.1"
                     /translation="ISEFVDGFETLNRIGPCVSIFGSARTKPGSKYYESAVQIARRLT
                     EEGYGIITGGGPGIMEAGNKGAYENNGMSVGLNIQLPFEQNHNPFIDPDKNLDHRFFF
                     VRKVMFVKYAQAFVVMPGGFGTLDEMFEVLTLMQTNKIKKVPVVLYGIEYWSGLKKWI
                     KEVVGEMEHNINPIDLDLMPITDDIEEVVRIINEYYEGDKQVRLRPNYEL"
BASE COUNT         3863 a         2760 c         2712 g         4258 t
ORIGIN      
        1 aatgaatact ggtggtaaga atgattttgg ctttggcgag gtaaggatgg taaaagccgt
       61 acatagcagt agtttgcctg atggtagtta tggtggaaat ccaacaggat ttctaataca
      121 tgctgatggc gtgaccttat attttgccgg tgatactgct ttgacaaaag atatggaact
      181 gattccgtcc ttttaccaca aacctgacct ggccattttg ccggttggtg gatgctttac
      241 aatggattat gaggatgccc tgattgcctc taaattatta caatgtgaca gagtcatagg
      301 gtgtcatttc aacaccttcc ctcctatctc catagataca gttgtggcgg aggaattatt
      361 tgccaaaaac ggaaaagaac tgattctgcc tgaaattaat caggtaatca acttataata
      421 catgggaaaa attgtcgcta tagctaatca aaaagggggt gtcggaaaaa ctaccatcgc
      481 catcaatctt ggagcatgtc tggcattgat ggaatataaa gtacttattg tggatgctga
      541 tccgcaggcg aatgcctctt caggaaccgg cgtgaccaag gaaatgacca attacaccat
      601 ctatgactgc tttattaacg aagtaccggc cgtggatgct attgttcatt ccaatacgcc
      661 gaatcttgac attctgcctt cagatatcaa tttagttggt gcggagctgg aattggtaag
      721 tcgcttcaaa agggaatata ttatgaaaga ctttttggag ccaatcaggg ataaatacga
      781 ttacattttt atcgactgtc tgccatcttt gggtatcata acggtcaatt cacttactgc
      841 agcggatagc gttattattc cggtgcaagc tgaaatattt tctttggaag gtttgggcaa
      901 gttgaagaac actatttcgt tggttcgcaa gcagttgaat ccccatctca ccattgaagg
      961 tatagtactg accatgtatg acaagcggct tcgactggcc aatcttgtca tcgaagaggt
     1021 gcaggaacat gctaaggata gggtgtttga cactataatc cacaggaact cgaggataag
     1081 tgaagctcca tccagtcatc agccggtgat tatgtatgac gcaggaagta aaggaaccat
     1141 caattttatc aatttggcac atgagttttt aaaatatcag ggtgctgcat ctgaacaagc
     1201 gactgtttaa ccatttaaaa cattagtttt gaaaaagaaa gatttaggaa aaggaataag
     1261 ggcattgttg aacagtatgg atcaggagga agctgaacca acaattgaag cggtaaggga
     1321 attgtcggat ggtgtggcta tgatcagaat ttctgaaatt gaaactaatc cggatcaacc
     1381 caggtcacaa ttcaatgacc aacagttgaa tgaattggcg caaagtatta aaaattttgg
     1441 aatcattcag cctttgaccc ttaggaagac cggaaggaac agatttcaaa tcattgccgg
     1501 agagcgtcgt tttcgagcag ctcaaatagc cggtcttcag gaagttcccg cttatatacg
     1561 tcttgccaat gaccaggagc tgtttgaaat ggcccttgtg gagaatatcc agcgtgaaga
     1621 tcttaatccg attgagattg ccatcagtta taagagattg atggaagaaa ccaatatgac
     1681 gcaagaaaat ctgtcagaaa ggattggaaa agaccgaagc acgatcgcca attatgttcg
     1741 attattgaaa ctattaccgg atattcaggc cactgtcaag gataagcggc tatccatggg
     1801 gcatgcaagg gctctggcag gtgtggatga tattgcgctt caacttgctt tttacaagca
     1861 agctatgtct aatgcgtggt cagttagaca gcttgaaaac aatatccgtg atcatttcca
     1921 tggaaaagag gtggcgaaac cgacatcaag agctaaaaac cctgaattgg agcatctgaa
     1981 agacagactt aagaaaaagt ttggtgtcaa ggtacaagtg gaacagaatg aaaaaggtca
     2041 gggatatttg aaatttattt tcaactctac aaacgaatta aacggtatct tggacgttat
     2101 agatggataa aagtcaaatc aatcagaata aaagctttta aaaacatcaa aatggccgat
     2161 atgcttcatc ctttagtaaa ggcaatagtg cctatagtgt tgatagtcat ttcggtacat
     2221 actttatttt gtcaaaatac tgattcatta tttttggctt cgccaaaaga tagctcgcat
     2281 cttacaaaag acacagttgt aacaaattgg gaaggtgttg atctcaaaac tcccaaaaaa
     2341 gcccttattc ggtccgccat tttacctgga ttgggtcagg cgtacaatca tcaatactgg
     2401 aagatcccta tcgtatatgc cggtttcggg gctttgatct atgccggaga atttaaccgg
     2461 aagaactatc gtttattaaa ggaagtttat agaaatatga ttgatggggt gccaactgat
     2521 tatgaccggt attcgagaca aagtgttcgg ctggcccggg acaactatcg aaagaatatg
     2581 gagctttctt atattgccct tgctggagtc tatggcttaa atatcctgga tgctttcgtc
     2641 tctgctcacc taaagacatt tgacatcaat gaaaatatca gcatgaaaat caagcctgct
     2701 gtacagcatg aatatattgg tctttatgct gtgccaaccg ggggattgac attttccttc
     2761 gtcctacatt aatcctataa gcttggcgta tttcatgatt tcaccgggat tggaaacttt
     2821 taacttgccg gtcattaacg ccatcattgg attagtttcc ttattgacaa gatcactaaa
     2881 gaccttatcg cttgttctga taaccgattt ggcatctccg attaaacctt cagaaacgtc
     2941 aattttccca tcagctattc ggagcgtgta gagtccgccg ttgtcaccgg tcacatcaaa
     3001 atgaaggatg gtattgacgc cttctaaagc tgcggggata actttatttg gcaattcatt
     3061 caggaaattt ttgatagagg tcattgcatt gggtttataa tagtaattta ttaaatgagc
     3121 agcaaagcta caatttttat cctatcggat tgccttcaaa atcaaattct gtaacctgtc
     3181 cgaaagctgt aagggaatta agtacttcac gactaccaat aaccgcgatg tgcatgttat
     3241 cagtcaataa atacttttgt gccatcagcc tgatatcatc gaccgacacc ttattgattc
     3301 taatgagctg attcttgaaa taatcttcag gtaaattgaa tttgtggcgt gcaaaggcaa
     3361 attttgcaat gatcattggg ttttcaactg accttgaaaa attaccgctg attacgtttt
     3421 ttacctgttg caattcttcc acagaaatat ccacatctct cagtgtattc atttccttta
     3481 caatttcaag aatggtgtcc gctgtcacat catttctgac gcttacggaa gtggtaaacg
     3541 agccaatgga ctcatcattg gtaagtcgtg aatatatgcc ataggtatat cctttgttct
     3601 ccctaagatt gaggttgagc ctactcgaaa aataaccacc taacacagaa ttcattaaat
     3661 tagcagcatc tgtgtcagca ttgtatggta aataatttat ggaataacca ataccaatga
     3721 gtgattggac tgcgccaggc ctttctacaa aactgacatt cacaccgttt ctcttcatat
     3781 cctcaggcca ttccaaagta gtaaccgacc tgggaatcca agatgaaaaa ttgtcaccca
     3841 ccagaatttt aatatcttcc atagtgatat caccttcaaa aatcaagtag gaattatcgg
     3901 gcgacgaatt agttaaaaac cattcacggc aatcatccac agtaatattt cttacggtct
     3961 tttctgtgat tacttcactg tagggatgcc tgccaggata aatccttctt ttgattagat
     4021 tgcctaaaat ggactccggt tcagattttt gtgcggccag atttgaaaga agctggcgtt
     4081 tcagattatt gaattcctct aaagggaaag ccggggttaa aattgcgtct gcaaagagtt
     4141 gaaatacctg tttgaaatat cgagtcaagg aagaggcggt tccaccaatc gaagtagtat
     4201 ggatgcttcc gccataatag tcgattttac gatcccattc atatttgcta tctcttaccg
     4261 taccacaaga taaaagttgc ccggccagct cctgcaatcc tgctttatcc ccatcagcaa
     4321 acacaccacg atcaatgtat aattgcacat tgactcttgg tagcttatga ttttcaacca
     4381 gaatgatttt tagcccatta tccagttgga aaaactgatc aggcctaaaa ttgatgggtc
     4441 cggctatttg aggatccggt tgttttttgc ggaattcttc gtcagtaatt gttcttttca
     4501 tcgtttcctt tattcaacct tttcaatttt gtgagcttca ggcaaccaga ataaggctac
     4561 cctattgtct ttattaaaat attttagtgc ggcttgtctt atatcttctt tttttactga
     4621 tctgtagaaa tccatttgtt tgttaaacat agatgtgtct ccaaagtagg tgtaatacgt
     4681 tgacaggcct tcagctatag aaagaagggt tgattgttcg ttgaccagtt ttgtttctgt
     4741 ctgattgatc agtttctcaa aatcttcatc ggttaatcct tcctttaaaa tgaggtctat
     4801 ttcttcatcc attgctgatt caagaacttc gggatcagta tctggatttc caatggcaaa
     4861 ttgtaacacg actccgggat gctccagttc cagaggaaaa gatcccactt gcacggcaat
     4921 tcttttttca tcgacaagca ccttgtttaa cctgctgctt tggccggtac ttaaaagatc
     4981 tgataatagt tttaccgcga aataatctcc tttggcaaaa gctggaatgt gataaagatg
     5041 aattacagca ggtaattgga tattgtcata tacgatgtca cgcaattcat gtgccatggg
     5101 tggttcattg ggttcaggac gattgatggg caattctcct ttcggaatgg taccaaaata
     5161 tttaaggatg agtccttttg cattatctgt atcaatatct ccggcaatgc acaaggcagc
     5221 attttgaggt acatagtacc ttgcataaaa attcctgtaa tcctcctcgc tggctgcatc
     5281 gatatggtcc attgagccaa ttaccggcca tcgatacgga tgtacagtat aggctcgttg
     5341 catgctttct tccacgatcc tcccatacgg tctgttgtca tacctttgcc ttctttcttc
     5401 cttcactaca gagcgttgtg tttcgattcc tgtctcatct acggtagcat gcaacattcg
     5461 ttctgactca agccagagcc ctgtttccaa ttggtgggaa ggaaagactt cgtagtaaaa
     5521 ggttcggtca taggatgtat tggcattcag agtaccacct gcattttgaa caatattaaa
     5581 aaattggcct cgggctatat tttcggagcc ttcaaagagc aaatgttcaa aaaagtgagc
     5641 aaaacctgtt ctgtccggac tttcattttt actacccaca tgatacatga cagaaatgga
     5701 aacaagcggc gaggtattgt ccttatgcag gatgacgtga aggccattgt ccaggtcata
     5761 ttcctcaaaa tcaatcgata aatgattctt cgacattaat ttaattattt caacagctat
     5821 ttacaataat ataataagcc aaaatgttta ttgaatcatg aataaaggtt aaaaatcttt
     5881 gtaaaaattg tttcttttaa atttgcatca accaattaat cagattttct ctttatttgt
     5941 gtttaaatac ataaatggca gtattcaaga cattggacaa aatgattatg aagggattta
     6001 tcattccttt cattgccact ttcttcattg ctctttttat tcttaccatg caattcctat
     6061 gggtctgggt ggatgatctg gtgggcaaag gtgtaggagc cctgattatt ttggaactat
     6121 tgttttatct gctcttatct ttctttccga ttgcatttcc aatagcagtt ctattaagtt
     6181 cggttatgct tatgggagga tatgccgagc gatatgagct ttcaagtatg acatcttccg
     6241 ggatttcact gatgcgtgtc atgaagccat tgatttttct ttctttcttc atagcctgta
     6301 tcagttttgg tttcagtaat tacctgtggc ctgttgccaa tctgaagtat cgatccaggt
     6361 tgctggatat caaaaatcaa aaaccgactt tgagcattca aaaaggcgta ttcaaccggg
     6421 actttaccgg ttatgtgata aaggtaacgg ataaagacaa agatggcaga gcagtcaaag
     6481 gaattatggt gtatgacaat cagcgtcaaa ttgacaagtt gaatctgatt actgccaagg
     6541 agggagagat gtatacagag ccaaaggaca atcttttcat catgaagatg ttcaatggac
     6601 atcagtatca ggaaatgaat ccacaaggga gtaatgaagg ccggtatcct tttgttcgga
     6661 tgaattttaa agaatatacc aaggtttttg acatgagtca atttgacttt aacaggacgg
     6721 atgaaaacct ttataaatct catcagagta tgctgagtat caggcagtta aaagttgccg
     6781 ttgacagcat caatagtaga cttgaccgag cagtagatga catgaacagt ggcttgatga
     6841 gttccatcaa ttccaaatat gccacaaccg gatattcagc cttcaatgat agaagggaaa
     6901 gtgtcgatca tcaatatttc ccaaagcggg atacccagtc aatggaaaaa ttgaagcgcg
     6961 agcgattggg tattattcct ataaaaccac aaagaaaaaa tacacctgct cagatagatg
     7021 aggattttca tagctccccg gcaaatttaa ttacttccag ggatttgttt gtaaatggca
     7081 attttaagcc cggaaaacct gtgtttgatc aattgaatgc ccgttttcgc ctggctagcg
     7141 tcaactatgc cgtatctttg gctaaaagtg ctcaaagtac tgcggatctt cagtcgagta
     7201 tctatgctgc cgaatatgac tctaaagtaa ggcatatctt cgagatgcat gtcaaatttg
     7261 cctatagtgt gatttgtatt gtttttctct tcattggagc gccaatggga gcaatagttc
     7321 gtaaaggcgg atttggttat cccttgcttg tcgccatcat tttttacatg acttaccagg
     7381 ttttgttcac gattatgaaa aaatcagctg aaagccaggt catccctccc gcattggcac
     7441 cttggattcc ggtaatggtc atggctttga ttggtttcgc tttaacacgc agagcaatga
     7501 gtgatggaag tatgaatctt gaagtttttt ccaagttctt cactaaaatt tttgataaag
     7561 catcagaaag ttattaccaa tttgtcgaat tttttctcag ttgggttaaa aaataggctc
     7621 aaatctcgtt atgatgttat caaatttctc tgagcgatgg actttggtcc ctactgacgt
     7681 attttattgt atgcccggtc taagacgcga tattcaggtt attctcctat caatgcacct
     7741 gagacattcc acgaactgaa gctgcttcct tccggtttac cctgaggtat tttgactgtt
     7801 aatacatgct ttccgacagg tagatcaccc aggtcgatga tatagggatt ggtaacagtt
     7861 cccggacacc agttagatcg actatagtcg gaggaggata acccattgga aaaattaccc
     7921 gataccggat tatagagtct gtaggtgccg cagtcttccc gccagggaat caattcgaat
     7981 ataattttat cattgagcgt aattgtattt tttttacgta cgaattcatc tcccctgccc
     8041 catccaccat gtccggtagt aataaaattt aatttgcaat tcttcatggt ctccttgagt
     8101 tcaaactcca ctttcagtcc tttttctgaa tcaaacatag ttccataatc ctgtccggcc
     8161 atctccatta cattcaatgt attaaataat ggtagtactt tgcgtgtggg catatccata
     8221 tcttcttcct tatgaatggt aaagttcatt gaaatgatat gtccaccctt gtcataattt
     8281 cctataaatg cgccgatgta cacctcctta tcgtttaatt caccggcata atccgaaact
     8341 tcctggcgat aggttattga atcaagccat gttttacctt tcagtgttac acggtctctg
     8401 aagtgaccta ttccaaaagg ggtaaaaaat ctcatcagtt caagtaaggg cgtatattgg
     8461 ggtgttagaa caaccccctg atattttttc ccatttccat tatcgaatat gggcagctcc
     8521 ccgatacctt tttgaagtgc atccaagaaa ctgacccttt tatctacagg aataaggaat
     8581 acggatccgg ttctgtcata tgcatcacca ttggatcgct gagtcatatc cagaaatatc
     8641 tggtcgtgtt gtccgatttt ggggaatctg acttttttta ccacgatggt tccatttgca
     8701 aatcgcataa cttcgttact gttatcaaaa gggccatctg taaagcatat ctgttggtgg
     8761 ttgaatacag gtatcgtaat aaaacggctt tgccatatcc tgtcattata atcgattttc
     8821 tccaggtatt ccggtgttac atttttcaga aaagccggcc attcgaatct ttcttttgaa
     8881 gttccgatcg cagaaatcct gtagttgccg tttcgtacca tttcgagaac gaatccaagg
     8941 cctgtgccta aaagcgtagg gcctccctta attttcattt catctgtgta atagatttca
     9001 atggtgttgg aattgatgct ggtccgggcc tttttgcacc ggtagccgag aatatcctta
     9061 gtgtcagaca ataactccca gttctgttta tttattgact ggctatccaa catggcaata
     9121 cagctacctt ctttaaggaa tgcatattga gcgtaactac tgatgttgct atgtattaat
     9181 gtcatttcaa aagggaatgc agccgtattg ttcatctgat ctctgttggt aatcagggca
     9241 tttccggaat cggcaaacac ccaaattgga ttttgattcg gatcagtcct gtcattataa
     9301 gaagtactat atacaacctt cgtcacttct ttatcggaaa atttgaattg tgctgtcact
     9361 gaagaggaca ggcaaatgac agcaaataaa agtaagaaac ggatgtggtt attcatcaag
     9421 ttcaatatta tcgtcaaaca atgtccatta tctttttcag gatatatgaa aaagcatgat
     9481 taattaatga aaatcaattc tttacaggta aaaaggaata ctttttacca tattccatca
     9541 tttgagtgaa gaaattatcc ggatactcta cttttacatc aataatctta tcacctttca
     9601 tgacaggtac atatctgggt tgaataaaac ctttgtaagg cttgatattc aattttttaa
     9661 aacgctccaa cacttctctg tgaagttcag gatccacctt gactccataa ttttcgacca
     9721 ggtcgtgacc ggctttgtaa tcaccctcag atttgattcg ttgtatttct cttagtaact
     9781 gtccgaataa ctgtcgaagt ttgttgtaat cgttgattct gacatatgtt ttgccatctt
     9841 tttttacaaa ttcaactaca ttggaagctt tcccattttc ataggcccat ttagcaacga
     9901 gctggcggtt tctcatatgg gcttcttcca ggttgttacc gggttggatt cttgtcaatt
     9961 gggtcatcaa accattcatc atgtatgaat cgtactctgc cttaccggca tcaacagttg
    10021 gcattacacc tatttcgacc aatttggggt ctatcatata atatagagca accaaatcag
    10081 ctctggcttc ctccagtgtt gaagcatagt tcttaagggt tttgtcagta gattcaacac
    10141 ctggattgat ctgtccggat gcatgtccga taacctcgtg catatcagta tgcaggtcac
    10201 tggtgatatc tccaaactct tttattctgg ctttcacttc atcattgaca acgaattcgt
    10261 cgagcatacc gcttgtagcg ccatcgacat tataagagtg gacaatgttg ctcagagata
    10321 cagattttga tccatgttcc tttctgatcc attcattatt gggaaggttg atgccgatag
    10381 ggccggcagg agctgttgcg ccggcttcca taagcaccgt gatggccttg gctatgatac
    10441 ctttgacatt cttcttttta tgctcaggca tcaagggcga attgtcttca aaccattgtg
    10501 cctgctttgc tattgcctgg attctttttg tggcttcaag atccttgata ctgacaacac
    10561 tctcataagt tgcctttcgt ccaagaggat cctggtagac ttcaatgaaa ccgttgacgg
    10621 cgtccgttct gcttgctgta tcattaaccc atgcaatgca atagttgtcg aaagtgtgga
    10681 ggtctccggt tttatagtaa tcaatcagta ggttaatagc ttttctctgt tgatcgttct
    10741 cggcgactcc ggatgctttc tctaaccaat agattatttt atcgatagcg ggaccataca
    10801 taccgcctga cttccatact ttttcaaaaa ccttgccatt ttcctttacc agtttactat
    10861 tcaagcccca catgggtgca ttacccttgc tgtcaaactt ttcataaaat aattcaacgt
    10921 ccctcaaact gacattttcg taaaaattaa cagaagaggc ctttacatta tcgatattag
    10981 gtcgcaaatc gaccaatttg ggttctacat ttggatcata aatgattggt ttgattcttt
    11041 caaggaaggt atcaaggtct tcaccactat ccattgggaa caaattgggg tcgctagctt
    11101 ttgcaatttc cttaaagtat tcaaaggaga cttcgggaaa gaatttttca ttgccataat
    11161 gatgatgatt gccattgcta aaccaaaaac gcccgcaata gatttcgaaa ttcttccagt
    11221 tttcggtaga cttatctcct gaataagttc cgtaaatagc ctcaagagtc ttcctaatgg
    11281 taagtccatt cttactctgt tggtcataga tgatgtctcg tcctgaaagt gcagcctctg
    11341 ataggtaata agcaagtatt ttttgttgta atggcaactg gtcaaatcca ggtacctgat
    11401 atctaagaac cttcagatca gcaaattcct gcgctgatac tttgaaatta ggatcgtagc
    11461 ccgaaacagt attttgtgaa tgttcgttct ttttagaatt attgggttct gagcaagaaa
    11521 aagcgattgt tcctgcaatt aaagtcaata gaaaatgttt gtatctcacc tttaatgaat
    11581 ttagaaaaag gctaaaatac aattttatat caatcactta aaatacaatg aaagaaaagt
    11641 aaggtaatta cttcctttga tgttggaaaa caacacagga ctatgtgtct ccactttaaa
    11701 aatgattcat cccacgctta atctttccga aaaaccaaat caagatcaga aaccgtatat
    11761 ctgcttgaga ttccattctt tcattgctgc ctttcttact gcttcggcta ccttgtaggc
    11821 cacttgatca tttagcgttg caggaataat attctctttt gtaggctgat caatacaatc
    11881 agcaatggca taggctgcgg ccattttcat ggagggagat atccttgggg cacgtacatc
    11941 caaagcaccc ctgaagattc cggggaatcc gagtacattg ttcacctgat tggggaaatc
    12001 acttctgcct gttcctacga ttgctgcccc accggcatat gcctcatccg gcataatctc
    12061 cgggatcggg ttggccaagg caaagatgat ggaatccttg ttcattgtgg caatatcatg
    12121 tctgtcaagc aaactggcac tgctgacacc tataaataca tcggcattcc ttattgcatc
    12181 cttgacagtc ccctgcatat tttcgctatt agagaatgcc aacagagatt tttttacatc
    12241 agtcagatct gttctgcttc tatgaatgat gcctttggta tcacaaatga tgatttcctt
    12301 gacaggctgg catactgaat tatcagtatg ttccacacaa cgcaggagtc tggctatggc
    12361 aatacctgca gcaccggcac catttataac taccctcatc tcactcaatg gcttatcaag
    12421 tagtttagaa gcgttgatca atcctgcaag ggtaacgaca gctgtaccat gttggtcatc
    12481 atgaaatacc ggaataccta aatcctggag agcgttttca atttcaaggc aacggggcgc
    12541 actgatatct tcaagattga ttccaccgaa tacaggtgca attcttttta ctgtatctat
    12601 gatctcctgg gtattctgag tatccagaca aatcgggaat gcattgatat tggcaaaacg
    12661 cttaaaaagc attgccttgc cttccataac tggtattgcc gccgcagctc cgatattacc
    12721 caaacccaaa acagccgaac catcagaaac tatggctaca gtattacctt ttagagtgta
    12781 atcgtacact gtctcaggtt tttcggctat ttcgaggcaa ggagcagcta ctccgggaga
    12841 atacaccagg gaaagatcgt ccctgttttt gatggtaaat ttagtctgaa cggaaatctt
    12901 tcctttcaac tgtttgtgca ggaatagagc ttccttaaaa taatccattg tgtttattat
    12961 tatagttcgt aattggggcg aagcctcact tgtttatccc cctcatagta ttcattgatt
    13021 atacgcacca cctcttcaat atcatctgtg ataggcatca ggtccagatc gattgggttg
    13081 atgttatgtt ccatctcacc aactacttcc ttgatccatt tcttcagacc tgaccagtat
    13141 tcaattccgt ataaaacaac cggtaccttc ttgattttgt ttgtctgcat caatgttaaa
    13201 acttcaaaca tttcatccag ggtgccaaat cctcccggca tgacgacaaa agcttgtgcg
    13261 tatttgacaa acatcacctt cctgacaaag aaaaacctgt gatctaaatt tttatcagga
    13321 tcgataaagg ggttgtgatt ttgttcaaat ggtaattgaa tgtttaagcc tacagacata
    13381 ccattattct cataggcgcc cttgttacct gcttccatga tgccgggtcc gccccctgta
    13441 ataataccgt aaccttcttc agtaagcctg cgcgcaatct ggacggcact ttcatagtat
    13501 ttacttcccg gttttgtacg ggcagaacca aaaatggata cacacgggcc tatcctgttc
    13561 agggtttcaa aaccatcgac aaattcggaa att
//
LOCUS       DAOQMC010000037        13418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1716_length_13418_cov_4.610941, whole genome shotgun sequence.
ACCESSION   DAOQMC010000037 DAOQMC010000000
VERSION     DAOQMC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13418
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_13418_cov_4.610941"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKV31_02690"
     CDS             complement(<1..304)
                     /locus_tag="PKV31_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91790.1"
                     /translation="MKSLYIYLAVIIFTPSYIFAQNCSVNAGLEGKWCAGQEIILDGA
                     VAGTVASGQLPTWSLVSGPGPVTIEDSTKVKTKAVATVAGEYVFRLSIVCGMGKTSQ"
     gene            complement(311..1159)
                     /locus_tag="PKV31_02695"
     CDS             complement(311..1159)
                     /locus_tag="PKV31_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNB91791.1"
                     /translation="MKTNLLFTLTFIFFTGLSYSQTSIATIESEMVLVEGGSMYIQGR
                     IVTLPSFYSSKFEVTQKLFEEVMGYNIVTWPQVNIHPMNPVGFRADEEPTFYPDFLIF
                     CNELNKMKGLPIRYYKDPDLTMPVTKSDLHTQFDSYEDYSNNSFRLPTATEWEFSYRG
                     GIKSMGFDYSGSNNIYDVAWIYSNAFGTLHNVGQLKSNELGIYDMSGNVAEVIFDTSP
                     SGFVTEQLPTSEIITHGLQCGHIWDGGGLSFTVGGCSVLFEASTTFCKIPGGGIVGLR
                     LFKDKK"
     gene            complement(1363..1773)
                     /locus_tag="PKV31_02700"
     CDS             complement(1363..1773)
                     /locus_tag="PKV31_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91792.1"
                     /translation="MAYMIETPGTTIESSLQDTVQAYVADAGQVIVHGFCRAVAGGCT
                     IRISPSTTHLYDCHSGHRSELILFERISAFPKWIYIPPNTDYGFTLIFSGLPKSCKFF
                     DLIEICPQGRGFRLLNITRNDMDVYYLDFTCASD"
     gene            complement(2138..3391)
                     /locus_tag="PKV31_02705"
     CDS             complement(2138..3391)
                     /locus_tag="PKV31_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB91793.1"
                     /translation="MGRNRKPLLFLGARQVGKTFLLQEFGKTEYKQLAYINFEQPNAP
                     RSLFETDFDADRIITVLNAYCSLKINAEDTLIVFDEIQAAPKGLTALKYFYENASEYH
                     VIGAGSLLGVTIHPEESFPVGKVDFMKLYPMSFYEFLLAMGEVGMAELIERKDLENLH
                     YFHLKLIQYLRYYLFVGGMPEAVKDFAENRDWGSTRHIQNQILASYRSDFSKHAPKEI
                     LPRITMVWDSIPAQLSKENKKFIYGVIKHGARAKEFELAIQWLTDAGLLHKVFNTSRA
                     ALPLVAYHELSAFKLFHNDVGLLGAMSRLNAKTIALGDTIFSEFKGALAEQYVFQQLS
                     QIETHDIFYHTFDNSKYEVDFLIQTDNNEIIPIEVKSGENVKPTSFRLFCEKSKPQTA
                     IRTSLTPYKKEKWMTNIPLYAIGTL"
     gene            complement(4320..4910)
                     /gene="coaE"
                     /locus_tag="PKV31_02710"
     CDS             complement(4320..4910)
                     /gene="coaE"
                     /locus_tag="PKV31_02710"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNB91794.1"
                     /translation="MLKIGITGGIASGKSFVASMFELLGIPVYYADKEAKRLIVEDSD
                     IRRRIIDLLGEESYRDDGSYNTQWVASVVFSDHKKLLALNAIVHPVVKQDYLLWHSRM
                     HAAYTLHESALILEGGFNYLMDRIIVVTAPIEMRLDRLISRDGISRKAGMQKIQSQSS
                     DEQKLKLADFVIKNDGHHSLINQVLDIHHALKRLSA"
     gene            complement(4903..5547)
                     /locus_tag="PKV31_02715"
     CDS             complement(4903..5547)
                     /locus_tag="PKV31_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNB91795.1"
                     /translation="MAYTIDTYYETEIKVKGSKFIAVIFPVDRVEDAMNRLNDVRTHH
                     PKATHHCWAYKVGCPVTGYRMSDDGEPSGTAGKPIFGQLEAFDVTNILCVVVRYFGGT
                     LLGTGGLIKAYKDSAKEVLALAILKEIRPEYLYKVTFDYVRMPLVMAAAKRFPGRIVD
                     KHLDQNPFLILSTEREDEDYLIPLFKSYVLGVEVDHAAALNPPDFKIEKYMQDA"
     gene            complement(5553..6470)
                     /locus_tag="PKV31_02720"
     CDS             complement(5553..6470)
                     /locus_tag="PKV31_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB91796.1"
                     /translation="MNKSQPVTTSQDNLWYQGLRTVMLGVAISLVLVMVKGIAGITGH
                     SYALVADAIESLSDVVSSLIVWVGLKYASRPPDMDHPFGHGRAETLVTFVVVGILVMT
                     AAYIIFQSIVNMRTPHQMPAPFTLVVLFLVILVKEAMYRYVLHQSKKTGSTSLRADAA
                     HHRSDSITSVAAFIGILVALILGDGYEAADDWAALFAAGVILFNCYRIFKPAWGEIMD
                     RQIYEEIVTKIKALSASVDGVLDTEKCYVRKTGLNYLVDLHIEVDGRIPVFEGHDIAH
                     RVQDKLKDAGIGIIYVAVHVEPKDNPHIF"
     gene            7447..8946
                     /gene="purH"
                     /locus_tag="PKV31_02725"
     CDS             7447..8946
                     /gene="purH"
                     /locus_tag="PKV31_02725"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNB91797.1"
                     /translation="MDLINVSSALISVYDKTGLDVLIRELADHHVTIYSTGGTFDYIT
                     AMGVPAIKVEDLTGYPSMLDGRVKTLHPAVFGGILARRNDDHLGQLKQHQLPVIDLVA
                     VDLYPFEETLKRTSDDQVIIEKIDIGGVSLLRAAAKNHHSVVVISRQSQYPIVAQWLK
                     ANKGSTTLEQRTLLGYESFAVTAHYDTAISRYFAQKAGKQQMLRYGENPHQKAYFTGD
                     YESKFTQLAGKEISYNNIVDIDGAIALMSEFKDNKPAFAIIKHTNPCGVALRDTIKDA
                     WDAALACDPTSAFGGIIICNKTIDVETANAIDEIFYEVLIAPDYETGVVEVLKKKKNR
                     ILLKLHSFEPAVEQQKSMLGGTLVQDADLAPVDDETLNQVTRKGIPGEKREDLLFGIK
                     CVKHLKSNAIAIVKDLQMIGAGVGQTSRIDALRQAIDKAGRMGFTVEGSVLASDAFFP
                     FADSVEIAHMAGIRYIVQPGGSVRDEDSIAYCDENDLGMYFTGTRHFKH"
     gene            8955..9683
                     /locus_tag="PKV31_02730"
     CDS             8955..9683
                     /locus_tag="PKV31_02730"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNB91798.1"
                     /translation="MNLTIDAGNSRVKYGIFSNDELAKVIVRDSLKSMDIEELYKEHQ
                     IKRSIICASRNLSPENLAYLQSRDTLILDENTPIPIKNKYRTPKTLGKDRLAGAIAAH
                     VAFPFANNVVIDLGTAMTINFINSKGEFLGGNISPGLNLRLQALHNNTDMLPLPSTDG
                     EFSLYGMDTEQALRNGAIHGILAEIEYYSQKYKELCSPLKIILAGGNSVFINHYLKEP
                     YVIKPDLVMEGLNEIIKFNEKESL"
     gene            9664..11004
                     /locus_tag="PKV31_02735"
     CDS             9664..11004
                     /locus_tag="PKV31_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91799.1"
                     /translation="MRKNLFNLFLFTICTLTAGIAQQLHNPFSIYGMGEFQDTDPTTV
                     VSNGWANQTFYDRFHVNTNNAASYPYLLSTSFEVGINARYSILNNGPINYKYWSGQLA
                     HMSLAFPVFSPYNELLEKRNRKVHWGMGIAVQPYSLVAYNYEFDVDDPNAGLVRNKHF
                     GNGGTYKMVYNNGVSYKDFSFGVGLHYIFGKIIRNDQIEFLDNSGAFGNITSKENEVY
                     VKGLQFNFSAMYNYILNPMTDGKKTDYDKQKRITVGASFQPMSKLKSRVDAVDFVYSR
                     QLGTSISDTLSYLQSNQEYKGNYNSTYGVGLSYQHGAKWQVNVNYDGGVYDQYNVKGL
                     EADYRNTGMFKIGAEWCPEPNSFNSFLKRSKYRLGFRTGTMPLAFGGEQTKVTAGVIG
                     YGFPVYVNRQISHVNIAFEGGQKTYSEKFKEKYFNIRLGFNLNDDYWFLKRKFD"
     assembly_gap    11407..11506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11531..11740
                     /locus_tag="PKV31_02740"
     CDS             11531..11740
                     /locus_tag="PKV31_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91800.1"
                     /translation="MLILQAFLQTAGQIIFNCELIFKNGFRLSPTHLTCRLLNIYLLQ
                     AKAPPMHLPSYYFREIRTDYTHTLT"
     gene            complement(11794..12912)
                     /locus_tag="PKV31_02745"
     CDS             complement(11794..12912)
                     /locus_tag="PKV31_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB91801.1"
                     /translation="MIEQIKNTVRSLVVKTITFNLNTLDDDGRDIYLVGTFNDWNPQD
                     ERFKMERVKKGKYRLKVKLDENFPFPFEYKYTKGGWENEELTARGLKTKNRVALKMRR
                     SYTDRVARFLCNGSCSEARFRPIIEKFDKFPFPYLGVVRTVRVMLPYNYNADPHKHYN
                     VLYLQDGQNLWDPKAPFGNWDLEHKMTQMAMAGKTEVIIVAIDHGEADRIREYTPISG
                     TRIGRGDGQKYLSDVAKAVKPFIDNRYRTLRGREYTGLGGSSMGGLITLYGGLFYPDV
                     FGKLMIFSPSLWLIRDAQTEIPRFYTPFPTKVYLYAGGKESASMLPSMKRLKKALEEQ
                     HFFEKLVDVKLTIDPKGRHNENKWGREIVAAITWLYYK"
     gene            complement(12994..>13418)
                     /locus_tag="PKV31_02750"
     CDS             complement(12994..>13418)
                     /locus_tag="PKV31_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNB91802.1"
                     /translation="DPSKVDRSAAYAMRHIAKNMVAAGLCNEVLVQVSYAIGVAEPCG
                     VYVNTYGTSRVNMTDGEIARKIQEIFDLRPYAIEQRLKLRNPIYSETASYGHMGRENR
                     TVEKVFNKGKANEKKVTVELFTWEKLDYVDKVKEVFGI"
BASE COUNT         4107 a         2884 c         2577 g         3750 t
ORIGIN      
        1 cctggctagt tttgcccata ccacacacaa tagataatct gaaaacatat tcaccagcta
       61 cagtagcgac agctttagtt tttactttag tagagtcttc aatagtcaca gggcctggac
      121 ctgataccag tgaccatgta ggtaattgac cggaagcaac cgtaccagca acagcaccgt
      181 caagaataat ttcttgccca gcacaccact tgccttccaa acccgcattc acactacaat
      241 tttgggcgaa tatataagat ggtgtaaaaa taataacagc taaatatata tataatgatt
      301 tcatattctc ttatttttta tctttaaata aacggagtcc aactatccct cctccaggta
      361 ttttacaaaa agtagtactt gcttcaaaaa gcacggagca acctcctaca gtaaaactta
      421 aacctcctcc atcccaaata tgcccacatt ggagtccatg tgtaattatt tctgaagtag
      481 gtagttgttc tgttacaaac cctgaggggg aggtatcaaa aattacttca gctacatttc
      541 ctgacatatc atatatacct agctcatttg attttaattg tccaacatta tgtagagttc
      601 caaacgcatt agagtatatc catgcaacat cataaatatt attactaccg ctataatcaa
      661 accccattga tttaatacct ccacggtatg aaaactccca ctcagtagct gttggaagcc
      721 taaaactatt gttgctataa tcttcatagc tgtcaaattg tgtatgtaaa tcagattttg
      781 taacaggcat cgttaaatcc ggatccttat aatacctgat tggcaatcct ttcatcttat
      841 ttaattcatt gcaaaaaatt aaaaagtccg gataaaaagt aggctcttca tcagctcgaa
      901 atccaacagg attcattgga tgtatattta cttgaggcca tgttacaata ttatacccca
      961 tcacctcctc aaataacttt tgtgttactt caaattttga ggaatagaac gaaggtaaag
     1021 tcacaatcct tccttgaata tacatactcc caccctctac cagtaccatt tcagattcta
     1081 tggtagcgat ggatgtctgg ctgtatgaaa gacctgtaaa aaatataaag gtcagtgtaa
     1141 atagtaaatt tgttttcatc cggtgttata taaatttaga aatagttgtt tctcttcttt
     1201 caaatataag gaatgaggtt gatttgttat aggcgaatcg ccataaacct gcctttttga
     1261 gccatgaaac gggtgttttt gcggatcatg cacccgtcaa caaatcaaca aaaggggttt
     1321 acttctcatc ggagtccatt cgaaaactgt gacatcccac acctaatccg aagcgcaggt
     1381 aaaatccagg tagtacacat ccatatcatt tcgggtgata tttaggagac gaaagcctct
     1441 gccttgcggg catatctcta tgaggtcaaa aaatttacag gatttgggaa gacctgaaaa
     1501 gattaaggta aaaccataat ctgtattagg aggaatatat atccactttg gaaaagcact
     1561 tattctttcg aagaggatga gttcgcttct atgaccggaa tggcaatcat aaagatgggt
     1621 tgtagaaggc gatatcctga tcgtacatcc tccggcaact gctctacaaa atccatgaac
     1681 gatcacctgg cctgcatcag ctacataggc ctgtacagta tcctgcaagg atgattcgat
     1741 ggtagtaccc ggggtttcaa tcatgtaagc cattttgaaa aagtaatttg acattaatat
     1801 atggtaattt tgaaaaaaga atgacacgat cacaccatgt atataagtta aattacgaca
     1861 aaaaaagttt cattctcttt ggcataaatg ccataattca gattttccaa tttctaacgg
     1921 attaacagga attactcaca atcgcctaca caccgcgacg aatcagtaat gggttgaacc
     1981 caataaaccc tttttaatac aagatcttcg atcaccagtt cgatctcagc agcatatccc
     2041 taataacacc gaatcaagtc gtgtagggac gaccgcataa aattcagctg tttatagata
     2101 aaaccatgat agatacaatg ataacagcgg atgtatatca taatgtaccg atagcataaa
     2161 gtgggatgtt ggtcatccat ttttcttttt tgtaaggcgt caatgatgtc ctgatagctg
     2221 tctgtggctt acttttttca caaaatagtc tgaaacttgt tggttttaca ttttctccgg
     2281 atttaacttc tatcggaatg atctcgttat tgtcggtctg tatcaggaaa tcgacttcat
     2341 atttgctatt atcgaaggta tgataaaaaa tatcatgtgt ttcgatctga gaaagctgtt
     2401 gaaacacata ttgttctgct aatgctcctt taaattcact gaatatagta tcgccaagcg
     2461 caatggtttt tgcattgagt ctggacatcg ctcccaataa ccccacatca ttgtgaaata
     2521 acttgaaagc tgacaattca tgataggcaa ccaaaggtaa tgccgccctg gatgtattaa
     2581 ataccttgtg cagaagaccg gcgtctgtga gccattggat cgccagttca aattcttttg
     2641 ccctcgctcc atgtttgatc actccatata tgaatttttt attttcttta gataattggg
     2701 cgggaatact atcccacacc attgtgatac gtggcaaaat ttctttcggt gcgtgttttg
     2761 aaaaatcgct acggtatgaa gctaaaattt gattctgtat atgacgggta ctgccccaat
     2821 cccggttttc ggcaaaatct ttaacagcct ccggcattcc tcctacgaat aaataatacc
     2881 gtagatactg gattagcttt aaatgaaaat aatgtaggtt ttccaagtct ttcctctcta
     2941 tgagttctgc catccccact tcacccatag ccaataaaaa ttcataaaaa gacatggggt
     3001 aaagtttcat aaaatcaacc ttgcctacag gaaaagactc ctcaggatga atggttactc
     3061 ccagcaatga ccccgctcca ataacatggt attcagaagc gttttcataa aaatacttta
     3121 gtgcggtcag ccctttggga gcagcttgaa tttcatcgaa tacaatcagg gtatcttccg
     3181 cattgatctt tagactgcag taagcattca acaccgtaat gatcctgtct gcatcaaaat
     3241 ctgtttcaaa caaacttctc ggggcattcg gctgttcgaa attgatgtat gcgagctgtt
     3301 tgtattccgt ttttccgaac tcctgtaaga gaaatgtctt tcctacctgc cttgcaccca
     3361 gaaaaagaag cggctttcta ttcctgccca tcttccattt tttcaagctg tttatcttat
     3421 gtctatacac ttttctctca atatttaagc tcaaaaatac acttttctct caatatttaa
     3481 gctcaaaatc acacttttct cccgttattt agacctaaaa ctacactttt ctcctgattc
     3541 taataaatct tagcaggtca aaagtttcaa aaaatgaaga atatacaacc ggcattttca
     3601 tcttttattt acacatttaa agcgtgaatg aaaggtcgca tatacggagc ttggttcctt
     3661 ccttgttggt atattctacc aaaaggccac gcctacagct tttgtaacag aaacgaccgg
     3721 aataaacaac agaggaatta catcgcgatg gattcaatat cctgcgcagt aaaaagcctc
     3781 gttagaggcg accgtttggt agtgtatcag ttataacacg aagtcaagtc ctgtagggac
     3841 gaccgtttaa aattcagatg cttatttata taaaaccatg aaggcattaa ggtggattaa
     3901 gttagtcatt cttaatgttc cttcatttct tattggttcg ctaaagacag ccataacaat
     3961 taagatgaat taagatgcaa ccccaatggg tttaatcaac agcctatacc tcttatctta
     4021 taccaggatg atttatataa tagtccatta aatccaggaa tactagtacc gggagtgaaa
     4081 caatccgggg ttccgggatg ccgaggttac agtcgtttag gccaatgaaa ttgatctatt
     4141 acaaatgtac cttcggtatt atgcttaaat cccctcaaaa aaacctgaaa tgcaaaatgc
     4201 gtttgatatg aagtattgtg taaagataag gcaaaatcat aatagattca atcccaataa
     4261 attgttaaag taattaaaac atttaagtac cccggtcaat gactcccggg attaacagac
     4321 tatgctgaaa gccttttcaa ggcatgatga atatccagaa cctgattgat cagggaatga
     4381 tgaccatcat tttttataac aaaatcggca agtttaagct tttgctcatc actggactgg
     4441 ctttgaatct tttgcatgcc agcttttcgg gaaataccat ctctgctaat cagacggtcg
     4501 agtcgcattt cgatcggtgc ggtgactacg atgatgcgat ccatcaaata attgaaacct
     4561 ccctccagaa tgagggcaga ttcatgcagc gtataggcag catgcatccg actatgccat
     4621 aatagataat cttgtttcac tacaggatgc acgatggcgt tcaaggcaag cagtttttta
     4681 tgatccgaaa atacaacgga cgctacccac tgtgtgttgt agctcccatc atcccgatat
     4741 gattcctcgc ccaataaatc gatgatacgg cgcctgatgt cgctgtcttc cacgataagt
     4801 cgttttgctt ccttgtctgc ataataaacg ggaatgccca gaagttcgaa cattgatgcc
     4861 acaaaggatt taccggatgc aatgccacct gttataccta ttttaagcat cctgcatgta
     4921 tttttcgatt ttaaaatcag ggggattcaa tgcagcggca tgatccacct ccaccccaag
     4981 gacgtagctt ttgaacaagg gaatcaaata gtcctcatct tccctttcgg ttgacaagat
     5041 gagaaaggga ttttgatcca ggtgtttatc gacaatccgg cccggaaacc ttttggcagc
     5101 cgccatcacc aggggcatac ggacatagtc aaaggtcacc ttatacagat actccggcct
     5161 gatctcctta agtattgcca aagccaggac ttctttagca gaatctttat aagccttaat
     5221 cagaccacct gtaccgagaa gcgtaccccc aaaatacctg acaacaacac acaaaatgtt
     5281 ggttacatca aaggcctcca actgaccaaa aataggttta ccggcagtac ctgatggttc
     5341 gccatcatcg ctcattctat atcctgtaac cggacaaccc accttgtaag cccaacaatg
     5401 atgagtagct tttgggtggt gtgtcctgac atcattcagg cgattcatgg cgtcttccac
     5461 tctatccaca ggaaaaatca cagcaataaa tttactgccc ttgaccttga tttctgtttc
     5521 gtaatatgta tcaatggtat atgccataat ccttaaaaaa tatgagggtt atccttcggt
     5581 tcgacatgca cggcaacata tatgatgcct atgccggcat ctttcagctt gtcctggacc
     5641 ctatgggcaa tatcatgccc ttcaaacacc ggaattctac catccacttc aatatgcaag
     5701 tctaccaggt aattgagacc tgtcttacgc acgtaacact tttcagtatc taagacacca
     5761 tcgaccgagg ccgacaatgc cttaatttta gtcacaattt cttcgtatat ctgccggtcc
     5821 ataatttcac cccatgcagg cttgaagata cgataacaat tgaaaagaat cacaccggca
     5881 gcaaagagag ctgcccagtc atcagctgct tcatatccat cgccgagaat caaggctacc
     5941 aaaatcccaa tgaaagcagc aactgaagta atggaatcac tccgatggtg ggcagcatcg
     6001 gcccgaaggg atgtagatcc ggtctttttg ctttgatgca gcacataacg gtacattgcc
     6061 tccttaacca ggatgacgag gaaaagtacc accaacgtaa atggcgcagg catttgatgt
     6121 ggggtgcgca tattgacaat gctttgaaag ataatgtagg cggcagtcat aaccaaaatt
     6181 cctacaacga cgaaagtaac cagtgtttcc gctctgccat gtccaaaagg atggtccata
     6241 tcgggaggcc gggatgcgta tttgagacct acccagacaa tgagagagct taccacgtcc
     6301 gataaagatt cgatcgcatc cgccactaag gcgtacgagt gtccggtgat ccctgctatt
     6361 cctttgacca tgaccagtac taagctgatg gccacaccga gcatgacggt cctcagaccc
     6421 tgataccaca aattatcctg gcttgtagtc acaggttgac ttttgttcat agggtgcaaa
     6481 gttatatgat ttactattta atcttgaggt taagaaggtg aaagtagatg tcgcaaaata
     6541 catttctctt aatcataaaa aggcaacccg gtaaaccata aaagggttat tgccaggaaa
     6601 aatgtgtatt taatagtaat tatatatgtt atatattatc aaaatattta acatgaaata
     6661 aatgtaaaac aaaaatcaaa tcagttaaca atgcataatg ccacttcaac tactgcatat
     6721 actaaataac cgggccaatt tcactaattt ctgatcagcc ctatagatgg gatgggatgc
     6781 gaggagcaag accggttacg cacagcatta atttttaata aaacctgaaa tttcttctgt
     6841 acaattgtgt accgctcaat tattttcaaa ataaaaccat attgtagaaa tgtcattaaa
     6901 gtatatagtg ttattcataa taataatatt tataatatat aaataaatgg aatattgaca
     6961 gataaatgtt atcattacgg tgactgaaag ctttcgtgtc aaggtatagc ggagcacctg
     7021 aacaatgtaa ggtgtctggg gaatgaacta tggcctgttt ggtggctgac gaggatagac
     7081 gaccagattt ccaaccaaat aattccccaa taatactgat ccttagaaaa tatataaaat
     7141 ggagaatcca attacaagac cattaacggc acaaggtcta aatcatcttc tcctgtttga
     7201 ttgcgttgct atgtaagatt aataaacaga aatccagggg aatttatacc aaagcatggt
     7261 aaaaaagtac aaatacagaa atatctaatt cataatcaat aaattaaata tttatataaa
     7321 aatatttatt aattaaaatc ttgctgaccg tgtattggtt gcaaacgttg aaactttagt
     7381 aaataattca aaataaaata tgttgacaaa gaaattatgt acttttgcac cttattttga
     7441 aaaggcatgg atttaataaa tgtttcctct gcactaattt cggtatatga caagacgggt
     7501 ttggatgtcc tgatcaggga gttggcagac catcatgtaa ccatttattc caccggaggc
     7561 acctttgatt atatcacagc gatgggcgta cccgccataa aggtggagga tttgacagga
     7621 tatccatcga tgttggatgg tcgtgtcaaa accctgcatc ctgctgtgtt cggcgggata
     7681 ctggcacgga gaaatgatga ccatctggga cagttgaagc aacatcaact gccggtcatt
     7741 gacttggtgg ctgtggatct ttatccattt gaggaaactt taaaaagaac cagtgacgac
     7801 caggttatca tcgagaagat tgacatcgga ggcgtatccc ttttacgtgc cgcagctaaa
     7861 aatcatcact ccgtcgttgt catcagtcgg caatctcaat atccaattgt tgcacaatgg
     7921 ctcaaagcaa acaagggttc aactaccctg gagcaacgca cactacttgg ttatgaaagt
     7981 tttgccgtaa ccgcgcacta tgacacagcc atctccagat attttgctca aaaggctgga
     8041 aagcagcaaa tgttgaggta tggagaaaat cctcaccaga aggcatactt caccggagat
     8101 tacgagtcaa aattcacgca gctggcagga aaagaaatat cttacaacaa tattgtggat
     8161 atcgatggcg ccatagcact aatgtcagaa tttaaagaca ataagccggc atttgccatc
     8221 atcaagcata ccaatccttg cggtgtagca ttacgtgaca ctataaagga tgcctgggat
     8281 gcagcacttg cctgtgaccc gacatcggca ttcggcggca tcatcatctg caacaagaca
     8341 atcgatgtgg aaactgcaaa cgcgatagac gagatattct atgaggtgct gatagctcct
     8401 gattatgaaa ccggagtcgt cgaagtacta aagaaaaaga aaaataggat cctgctcaaa
     8461 ctacattcat ttgaaccggc tgtcgagcag caaaaatcca tgttgggtgg aactctcgta
     8521 caggatgccg acctggcacc ggtagatgat gaaacattga atcaggtgac caggaagggt
     8581 atccccggtg aaaaaaggga ggatttacta ttcggaatca agtgtgtcaa gcatcttaaa
     8641 tccaacgcca ttgcaatagt caaggatctc cagatgattg gagccggtgt tggtcagaca
     8701 agcaggattg atgcacttcg acaagccatc gacaaggcag gtcggatggg gtttactgtt
     8761 gagggatcgg tactagcatc cgatgctttt ttcccttttg ctgatagcgt ggagatagca
     8821 catatggcgg gcatccgtta cattgtacaa cccggcggct cagtcaggga tgaagattcc
     8881 atcgcatact gtgatgagaa cgatctgggg atgtatttta caggcacacg tcatttcaaa
     8941 cattaaacaa aacaatgaat ctgacaatcg atgccggtaa cagccgggta aaatatggaa
     9001 tcttttccaa cgatgaatta gcaaaagtta tcgtcaggga ctcgttaaag tccatggata
     9061 tagaagagtt gtacaaggaa catcagatca aaaggagtat catctgcgct tcccgcaatt
     9121 tgagtccaga aaacctcgct tatctccaat ccagagacac cctcatcctc gatgagaata
     9181 cgccaatccc gataaaaaac aaataccgta ccccgaagac tttaggaaaa gacaggctgg
     9241 ccggagctat agcagcgcat gtggcattcc cttttgccaa taatgtcgta atcgatcttg
     9301 gaacagccat gacaatcaac tttatcaaca gcaaaggtga atttcttggc ggtaatattt
     9361 caccgggtct taatctcaga ttgcaggcat tgcacaataa taccgacatg ttgcctctgc
     9421 ccagtaccga tggggaattt agtctgtatg gcatggatac cgagcaagcc ctcagaaacg
     9481 gagccataca cggtattctt gcagaaatag aatactatag tcaaaagtat aaggaattgt
     9541 gttctccatt aaaaattata cttgctggag gaaattctgt atttatcaat cactatttga
     9601 aggaaccata cgttattaaa ccggaccttg taatggaagg ccttaacgaa ataatcaagt
     9661 tcaatgagaa agaatctctt taaccttttt ttatttacta tctgtacgct aactgccgga
     9721 atcgcccagc aattgcacaa tcccttcagt atatacggaa tgggggaatt tcaagatacc
     9781 gatccaacaa cagtcgtttc caatggatgg gccaatcaga ctttttatga ccgattccac
     9841 gtaaatacca acaatgctgc atcctacccc tatcttctaa gtacatcctt tgaagtgggc
     9901 attaatgcaa gatatagtat actgaacaac ggcccaatta actacaaata ctggagtggc
     9961 caattggcac atatgtcgct ggcattcccg gttttcagcc catataatga attactggag
    10021 aaaagaaacc ggaaagttca ctggggcatg ggtattgctg tacaacccta tagtctggta
    10081 gcttataatt atgagtttga tgtagatgat ccaaatgcag gattagtcag aaacaagcat
    10141 ttcggtaatg gaggtacata caagatggtg tataacaatg gagtttcgta caaggatttt
    10201 tcatttggtg taggcttaca ttacattttt gggaaaatca tcagaaatga tcagattgaa
    10261 ttcctggaca acagtggtgc atttggcaac attacctcca aggaaaatga agtgtatgtc
    10321 aaaggtctgc agttcaattt ttcggccatg tacaattaca tcctcaatcc tatgacggat
    10381 ggcaagaaaa cggattacga caaacaaaag cgaatcacgg tcggcgcatc ttttcaaccc
    10441 atgtccaaac ttaaatcaag ggtagatgca gtggattttg tatatagcag gcagttaggg
    10501 acttccatct ccgatacatt gtcatatctc caatctaatc aggaatataa aggcaactat
    10561 aattcaactt acggagtcgg attatcctat cagcatggtg caaaatggca ggtaaatgtc
    10621 aattacgatg gaggggtata cgatcagtat aatgtaaaag gccttgaggc tgattaccgg
    10681 aataccggaa tgtttaaaat cggagcggaa tggtgccctg aaccaaactc cttcaattca
    10741 ttccttaaga ggtccaaata tcgattgggc ttccgaacag gaacgatgcc actagctttt
    10801 ggaggcgagc agacaaaagt tacagcagga gtgatcggat acggttttcc tgtatatgtc
    10861 aaccgacaaa tctcacacgt caacatcgct tttgaggggg ggcaaaaaac ctattcagag
    10921 aaattcaagg agaaatattt taatattcgg ttaggattta acctgaatga tgattattgg
    10981 ttcctgaaga ggaagttcga ttaaaggcct tgatagaata ccggaagatc ggtataagac
    11041 aagggtaata gtctttgata tcttaagatg ccagaaacag gatatcgcca aaacagtaga
    11101 ttatatagat ttgcctacat cacgtatagg tgctggggat ggagtcgtgt tgatacgctt
    11161 gatgggagtt gggtatgcaa cctgaagtag gtacgaaaat aatactcccg aatgaagatt
    11221 aggaggaaat tatttgtgct aaaatgaaaa aaagaaatca ttttaacgat tgttttgaaa
    11281 tctgtttcaa agaaagaccc gtaagtacga attcttaaag tattaaacat ctacctactt
    11341 caggggaacg ttgtactgac gcgtctttcg tcatccgggg ggattcaaaa atcgaagtat
    11401 gtacatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatg tacatgatta
    11521 ataactccga atgctgatcc tacaagcctt cctgcaaact gccggacaaa ttatttttaa
    11581 ttgtgagttg attttcaaga acggcttccg cctgagcccg acgcacctga cttgtcgcct
    11641 attaaacatc tacctacttc aggcaaaagc cccaccaatg cacctcccat cctattattt
    11701 cagggaaatc cgcacagatt atactcatac ccttacatga agagcatttc ccttcttcag
    11761 gactccctcc taaatctccc tataacttgt cgattactta taatacaacc acgtgatagc
    11821 agctacgatc tccctacccc atttattctc attatgccgg ccttttgggt caatggtcaa
    11881 cttgacatcg acaagcttct caaaaaaatg ctgttcttcc aatgccttct tcaaccgttt
    11941 catactaggc aacattgacg cactctcctt cccccctgca tatagatata ccttggtcgg
    12001 aaatggagtg taaaaacgtg gtatctccgt ctgggcatcc cggatcaacc acaacgaagg
    12061 gctaaaaatc atcagctttc caaatacgtc cggataaaat aaacctccat acaaggtaat
    12121 caaccctccc atagaactgc cgcctagtcc ggtatactca cgaccacgca aagtcctgta
    12181 ccggttgtca ataaaaggct tgacagcctt agcaacatcg ctcaggtact tctggccatc
    12241 accacgccca atacgagttc cgctgattgg agtgtattct cttatccggt cagcttcacc
    12301 atggtcgatg gccacaatga tcacctctgt ctttccggcc atagccatct gtgtcatctt
    12361 atgttccaga tcccagttgc caaaaggggc tttcggatcc caaagattct gaccatcctg
    12421 aaggtaaagc acattgtaat gcttgtgagg atcagcatta tagttgtagg gcaacatgac
    12481 acgcacagtc ctcacaacac cgaggtatgg aaaggggaac ttgtcgaact tctctatgat
    12541 gggcctaaat ctggcttctg aacaagaccc gttgcataga aatcgtgcta ctcggtccgt
    12601 ataactcctt cgcattttaa gagccaccct attcttggtc ttcaatcctc gcgcagtcaa
    12661 ttcctcattc tcccagcctc ctttagtata tttatattcg aatgggaagg gaaaattttc
    12721 gtccaactta accttaagcc ggtacttacc cttcttgaca cgctccatct taaagcgttc
    12781 atcttgagga ttccagtcat tgaaggtacc cacaagataa atatcacgac catcatcatc
    12841 caacgtattg aggttgaacg tgatggtctt gacaacaaga ctccggaccg tattttttat
    12901 ttgctctatc aaaagaaatc tgtaaaaaaa tgcatctacc cggccattac ctaccagaca
    12961 gatgcattca attctttatt gattatcagg caattaaata ccgaatactt cttttacctt
    13021 atccacgtaa tcgagttttt cccaggtaaa taattcaacg gtaaccttct tctcattagc
    13081 cttgccttta ttgaatactt tctcaaccgt cctgttttcg cgtcccatgt ggccatagga
    13141 tgccgtctcg ctgtatatcg gattacgcaa ttttaaacgc tgctcgatcg catacggtct
    13201 taggtcgaat atttcctgta tcttcctggc aatttcacca tcggtcatgt tgaccctgga
    13261 agtaccgtag gtattgacat acacaccaca aggctcggca acacctatcg cgtaagatac
    13321 ctgaaccaat acttcattgc aaagacctgc tgcaaccata ttcttggcaa tatgcctcat
    13381 tgcataggct gccgaacgat ctaccttcga agggtctt
//
LOCUS       DAOQMC010000038        13084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1791_length_13084_cov_5.113362, whole genome shotgun sequence.
ACCESSION   DAOQMC010000038 DAOQMC010000000
VERSION     DAOQMC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_13084_cov_5.113362"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKV31_02755"
     CDS             complement(<1..298)
                     /locus_tag="PKV31_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNB91803.1"
                     /translation="MATLNFGGVQEEVVTREDFPLEKAKATLHDETMAVIGYGGQGPG
                     QALNLRDNGFRVIVGQRRHSASWDKALADGWKEGSTLFDIEEACARGSILMNLLS"
     gene            complement(318..839)
                     /gene="ilvN"
                     /locus_tag="PKV31_02760"
     CDS             complement(318..839)
                     /gene="ilvN"
                     /locus_tag="PKV31_02760"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNB91804.1"
                     /translation="MEKTFTVSIFTENSIGMLNRITIIFTRRHLNIDSITASESEVKD
                     VHRYTITLKTTREKIDKVLGQIEKLVDVIKAFVHEENEIVFQELALYKIKTASISNGE
                     VEQVIRDHHARILSVDPLFMVVEKTGHVADTQLLFEKLKPFGILEFARSGGVAVTKPM
                     KELSTYLKELKVK"
     gene            complement(859..2547)
                     /gene="ilvB"
                     /locus_tag="PKV31_02765"
     CDS             complement(859..2547)
                     /gene="ilvB"
                     /locus_tag="PKV31_02765"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNB91805.1"
                     /translation="MEKLTGAQAVIQVLLTEEITTVFGYPGGAIMPIYDALFDYSDEI
                     HHILVRHEQGAIHAAQGYARVTGRTGVVMATSGPGATNLITGLADAMIDSTPLVCITG
                     QVSSKLLGTDAFQETDVIGISMPVTKWNCQITRTEDIPSIMAKAFYIARSGRPGPVLI
                     DITKDAQFGVSDFSFEKCTHIRSYNPTPEIDYSSLEAAAELINQAKKPMMVIGQGIML
                     SEAEEALRTFADKSGIPVASTLLGLGAFPCNHPLFVGFVGMHGDYAPNIKTNECDLLI
                     GIGMRFDDRVTGDTSRYATQATIIHIDIDASELNKIIPTDVPVHADAREALLFLTHLV
                     KERSHESWVNEFKEAKQTEVETLSKGYANCDTQDVRMDEVIKLLSNLTHGESIIVTDV
                     GQHQMMAARYYQYNKTKSNVTSGGLGTMGFALPAAIGAKLGMPDKDVIAIIGDGGFQM
                     TLQELGTILQSKIPVKIVILNNNFLGMVRQWQEMFFERRYSFTEMISPDYVKLAESYG
                     IQAKSIASKTDLEDGLREMLDAKEAYLLEVKVVKEENVFPMVPTGASVSEIRLA"
     gene            complement(2584..4260)
                     /gene="ilvD"
                     /locus_tag="PKV31_02770"
     CDS             complement(2584..4260)
                     /gene="ilvD"
                     /locus_tag="PKV31_02770"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNB91806.1"
                     /translation="MQPINKYSRTVTQDDTQPAAQAMLYGIGLTEEDLKKAQVGIVST
                     GYEGNTCNMHLNDLAKSVKSGVLQEDLVGLIFNTIGVSDGISNGTAGMRFSLVSRDII
                     ADSIETVVSAQWYDAVVAVAGCDKNMPGTIMAMGRLNRPSLMVFGGTIHSGQWKGQSL
                     NIVSAFEALGKKINHDITVEDIKGIIRHACPGAGACGGMYTANTMASAIEALGMCLPY
                     SSSAPAMSAAKQDECLAVGRAVRNLLEKDIKPSDIMTRRAFDNAIRMVIVLGGSTNAV
                     LHLIAMADSVGIQLTLDDFQRMSDSTPVLADLKPSGKYLMEDLHQVGGTPAVMKYLLD
                     HGLLYGDCMTVTGHTLAENLASLPELSSGQDVLWPICNPVKTSGHIHVLYGNLATEGS
                     VAKISGKEGQYFEGPARVYDDEYKMIAGLSNGEIKPGDVIVIRYCGPRGGPGMPEMLK
                     PTSAIMGAGLGNQVALITDGRFSGGTHGFVVGHISPEAYDGGTLALLRDGDIITIDAL
                     HNTIDVHLEEEVLQRRKAEWVLPELKARNGILFKYAKCVSGASTGCVTDK"
     gene            4828..6474
                     /locus_tag="PKV31_02775"
     CDS             4828..6474
                     /locus_tag="PKV31_02775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB91807.1"
                     /translation="MIFRSRFAIFLFILALCIITTGCKKTKPDEIHFDTKKNFWEYIE
                     KGNEIYSKKSDLKVFGKAKNYFDSAMLIAEHLKDTMLLAEATYAYGKVFDAWNSNPAL
                     TLAYYKKAEELFAKSDSVEAALYMKFHVAHAYNGVKDSTNCIKQINEILGIIDTLPGS
                     VANEIRFLPEVAMEAANAGDFDLAETILDKYVNKSNLKNDTLTLNHKDHYYYTRAVIQ
                     IQKNISTSSYLDTLEQVFQEARTTGDSMFYASVLSKFYEKTGNFKKSLYYSGLNYDMT
                     FRFINKHEYVNLGQKLAEMELNALEQENQIETKSFIILFVSITALALLSLASIYLNVI
                     IGRSRLKHIRVSKELEESHDKISLLYKELHHRIKNNLHMIFSLLQMQERKATVKATIE
                     NLRAARLRIESIAVLHEEMMRETYLVDFRDFIERMIEIIKDCFSYSHEIKTNLDFNDV
                     EIPLKQSFSLALIINEWITNSIKHANTSGKPLAIYINFTEIDDKIKVEYWDNGTVDIR
                     IQPQTGFGSEIITLLAKQIGKLMITDQSNPYYYAILINKN"
     gene            6515..6925
                     /locus_tag="PKV31_02780"
     CDS             6515..6925
                     /locus_tag="PKV31_02780"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HNB91808.1"
                     /translation="MKEELLKIYNSTTYLVPSLDLEIRPGIQNSRLNEVLAHYGVNTF
                     SFITAYNPNGIETLGRLNREAHNRLIVEVSRLGLQYVEGYGRGSNGWKEEISLLILGI
                     SLVESGILSQKFKQKAFVFGQINEAPSIIWSEPD"
     gene            7139..8617
                     /locus_tag="PKV31_02785"
     CDS             7139..8617
                     /locus_tag="PKV31_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB91809.1"
                     /translation="MSNNQSGTIWSQFLTLITVFFFWGFVAASNDILIPVFKENLHLS
                     QTQSQMVSFAFYTAYTVGSLIYFFISKMRGGDILNKIGYKNGIAIGLVISALGTLMFI
                     PAANTHSFNLFISGLFIVGLGFSLQQTAANPLAIVMGDPKLGSQRLSMAGGVNNFGTT
                     IGPLLVNFAIFGSIYAATAQADITSIKIPYLILGAAFILVALLIKFSKVPNEIDLDQM
                     AENEAEGDSSKVRHLSGPMKYPQLSLGMLAIFVYVGVEVSTASNLPEFMKQHIAGPDG
                     GVFPTDKIAPYISLYWASLMMGRWTAASEAFDLSMSMKRMLRYFMPYIAFGVFLLVNK
                     IANHDITPFYFYVFIIAAMVAGNIMSKGNPARQLLIYSCLGIIALIAGMLADGWVSTF
                     AFISVGLFCSTLWPCIFTLAIAGLGKHTNEGSSLLIMMIMGGAFVSLFQGYLSDHVTG
                     IQYSYIAGVLCFAYLAFYAIRTKNILKAQGIDYDHGVVSGGH"
     gene            8643..9623
                     /locus_tag="PKV31_02790"
     CDS             8643..9623
                     /locus_tag="PKV31_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNB91810.1"
                     /translation="MSSTEDLAIGIDIGGTNTKFGIVNRNGKVFHRGEIKTRKHAEVA
                     PFIDELYLSILPMIEYAGGPARIRGIGVGAPNGNFYNGTIEYAPNLSWKGVIPLAKMF
                     REKFDMDVILTNDANAAALGEMSYGAARGMRDFIVITLGTGVGSGIVANGHMIYGHDG
                     FAGELGHTMIIPEGRYHPGTGLYGSLESYASATGVARTAVEFLNSRPTVESLLRQYAN
                     EEITSKVVFDCARKGDSLSKEVYSYTGKILGMALANFAMATSPEAIILFGGLCNAGDM
                     LFGPVTDSFEKHLLPLFRNKIKILPSELPDDDAAILGASSLVWDKERGGN"
     gene            10336..11460
                     /gene="dnaN"
                     /locus_tag="PKV31_02795"
     CDS             10336..11460
                     /gene="dnaN"
                     /locus_tag="PKV31_02795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNB91811.1"
                     /translation="MILRISSGQLYKMLQVGQNAISSKPPYPILADFLFSMDGDKLSI
                     TSTDLEFTIISETTITPSDTGQFAFPAEILVSTLKEMPEQPIELIFEEADNMLKMISA
                     YGNYRSSSNSIEDYPEIPEIRTESQINIPASRFLKAISTCVFATATDEMKANMLGIAI
                     NIQPDAVIVAATDAHKLVKYTIFHDNGENESELVIPKKAVSVLRHIIEDDENLQINYD
                     RNHIFFEYKNYKVFCKLINQKFPNYNSVIPINNDKDVFINRQEWLQSLKRITLYGNRT
                     TYQTSFHVEENEIKINTIDPDFSNEASEVIRCQYTGEPLDLSYNAKFLIDSLSNLDAE
                     EVKLSISRPNRAAILTPKEDKPGENILMLVMPVMTKRLTE"
     gene            11469..12056
                     /gene="nadD"
                     /locus_tag="PKV31_02800"
     CDS             11469..12056
                     /gene="nadD"
                     /locus_tag="PKV31_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNB91812.1"
                     /translation="MNHRIGLFFGSFNPVHVGHMIIANHLVNETDLDQVWMVVSPQNP
                     FKEQKNLASDRFRYHLVYLAIGDNPKIMASDIEFGLPRPSYTIDTLAYLHEKYPEKEF
                     CLIMGGDNVSTLNKWKNSELLLERYRIYVYMRPGYDLGPLGQHANIIPLEAPLLDISA
                     TYIRQLLVSGKSVRYLLPEAVRMELEKSNPYRDLQ"
     gene            12063..12932
                     /locus_tag="PKV31_02805"
     CDS             12063..12932
                     /locus_tag="PKV31_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB91813.1"
                     /translation="MKRAYLILHIAVFLAGFTGIFGKLISLSQFPLVWFRVLFSVLIL
                     FLGLRLLKIRIGGGKAQIFKIATVGQLLIIHWVFFYGSIKYANVSVAAICYSLTSFFT
                     AIFAPIINRKKLQFGQLALSSITLSGIGLIFHFDTTFHTGIILGVISSAFAALYTITN
                     EKLVRIHDSRLINLYQLSGGVITLGLFLPFYYHFYPEQAFLPGGMDVVYLILLAAFCT
                     VALYLLFAEALKHLSAFSVNMAFNLEPIYAIGLAFLIFREGHEVNESFYWGLALVVLS
                     VFLQTLMNFYGRR"
BASE COUNT         3745 a         2752 c         2732 g         3855 t
ORIGIN      
        1 cagagagtag attcataagg atggaacccc gggcgcatgc ttcttctata tcgaataagg
       61 tgctgccttc cttccatcca tcggcaagtg ccttatccca gctcgcacta tgacgccgct
      121 gaccgactat gacccgaaaa ccattgtctc tcaggttaag tgcctgaccg gggccttgcc
      181 ccccataacc gatcaccgcc attgtttcat cgtgtaaagt tgctttcgct ttctctaagg
      241 ggaagtcttc acgtgtaacg acttcttcct gcacgccgcc aaaatttaat gttgccataa
      301 gtattatttt gttgatttta ttttactttt aattctttca gataggtgct gagttctttc
      361 ataggcttgg taactgctac accgcccgag cgcgcaaact ccagaatgcc gaatggtttt
      421 agtttttcga agagcaattg ggtatctgcc acatgacctg ttttttctac taccataaat
      481 aatggatcta ctgataatat gcgtgcgtga tgatcacgga ttacttgttc aacttcgcca
      541 ttagagatgg atgcagtttt gatcttgtac agggccagtt cctgaaaaac aatttcattt
      601 tcttcatgga caaaagcctt gatcacatct accagttttt ctatttgtcc caatacttta
      661 tctattttct cacgtgttgt ttttagcgta atggtatagc gatgcacatc tttaacctca
      721 gattctgaag cggtaatact atcgatattg aggtgtcttc ttgtaaatat gatggtaata
      781 cgattcagca tgcctatgct gttctcggta aaaatggata ctgtaaatgt tttttccatt
      841 ggataaaata ttatattgtt atgcaagtct gatttctgaa acggaggcac cggtaggcac
      901 catggggaat acattttctt cttttactac tttcacttcc agtaaatatg cttctttcgc
      961 atccagcatc tcacgcaagc cgtcttccag atctgttttg gaagcaatac ttttcgcttg
     1021 tataccatag gattcagcta gttttacata atccgggctg atcatttcgg taaacgagta
     1081 gcgtctttcg aagaacattt cctgccactg tcgcaccata ccgagaaaat tattgtttag
     1141 tatgacaatc ttcacgggta ttttactctg gagtatggtg cccagctctt gtaaagtcat
     1201 ctgaaatcct ccatcaccga tgatggctat tacgtcttta tcgggcatgc ccagtttagc
     1261 acctatcgct gccggaagtg caaatcccat ggtgccgagt ccacctgatg taacattgct
     1321 tttggtttta ttgtattgat agtatcgtgc tgccatcatc tgatgctgac ctacatcggt
     1381 aacaatgata gattctccat gagttagatt ggataaaagt tttatcacct catccattcg
     1441 cacatcctgt gtatcacaat ttgcatatcc ctttgatagc gtttctacct ccgtttgttt
     1501 tgcctcttta aattcattaa cccatgattc atgactcctt tcctttacga gatgtgtaag
     1561 aaaaagtagt gcttcccttg catccgcatg gacgggcaca tcggtgggta tgattttatt
     1621 gagttctgag gcatcgatgt ctatgtgaat gattgttgcc tgtgtggcgt agcgagacgt
     1681 gtctccggtt acacggtcat caaatcgcat tcctatacca atgagcaggt cgcattcatt
     1741 ggtcttgata ttgggcgcat aatcgccatg catacctaca aagcctacaa acaatgggtg
     1801 attgcatggg aaggcgccta aaccaagcaa ggtagaagcc accggaatac ctgatttgtc
     1861 tgcaaaggtg cgcaatgctt cttcggcttc agaaagcatg attccttgtc cgataaccat
     1921 cattggtttt ttagcctgat tgattagttc agctgctgcc tcgaggcttg aataatctat
     1981 ttcaggtgta ggattataac tcctgatgtg ggtgcatttt tcaaatgaaa aatcggatac
     2041 accaaattgt gcatctttgg taatatcaat caatacgggg ccgggacgac ctgaccgtgc
     2101 gatgtagaaa gcttttgcca ttatggatgg aatatcctcc gttctggtga tctggcagtt
     2161 ccacttggtg accggcatag aaattcctat gacatccgtt tcctgaaagg catctgttcc
     2221 gagaagctta gacgatacct gccctgtgat acagaccagt ggtgtagaat cgatcatcgc
     2281 atcggctaat ccggtaatga ggtttgtggc acccgggcct gatgtggcca tcactacacc
     2341 tgtccttcct gttaccctgg catagccctg ggcagcatga atagccccct gttcgtgtct
     2401 taccaggatg tgatggatct catctgagta gtcaaagaga gcatcataga tgggcatgat
     2461 ggcaccgccg ggataaccga aaacagtagt aatttcttcg gttaataaga cttgtattac
     2521 tgcctgagca cctgttaatt tttccattgt tctgtatatt tatctggatt tatataattg
     2581 attttattta tctgtgacac atccggtaga agctccggaa acacatttgg cgtatttgaa
     2641 taaaattcca ttccttgctt taagctccgg taggacccat tccgccttcc ttcgctgaag
     2701 tacttcctcc tcaaggtgaa catcgatggt attgtgcagt gcatcgatgg tgataatgtc
     2761 accatcccgg agtagagcta aagtgccacc atcataagct tccggagaga tatgacctac
     2821 aacaaagccg tgagtacccc ccgaaaagcg gccatctgta atcaatgcta cttgattgcc
     2881 caggcctgct cccatgatag ccgatgtcgg tttgagcatc tcgggcattc ccgggccgcc
     2941 cctgggtccg cagtagcgaa tgacgatgac atcgcccggt ttgatttcac catttgacaa
     3001 tccggctatc atcttgtatt catcatcata gactctggca gggccttcaa aatattgtcc
     3061 ctccttgcca ctgattttcg ctacacttcc ttcggtggca aggttgccat acagcacgtg
     3121 tatatgtccg ctggtcttaa ccggattgca aatcggccat aacacatctt gtccggagga
     3181 taactcagga agagaagcaa gattttccgc cagggtatga ccggttaccg tcatgcagtc
     3241 accgtagagg aggccgtgat ccaacaaata tttcataaca gcgggagtgc cacctacctg
     3301 atgaaggtct tccatcaggt atttaccact tggcttaaga tctgccaaaa cgggcgtgct
     3361 atcactcatg cgctgaaaat catctaaggt aagttggatg cctactgaat cggccatagc
     3421 aattaaatgc aataccgcat ttgtagagcc acccagcacg atcaccattc ttatcgcatt
     3481 atcgaaagcg cggcgggtca tgatatcaga tggttttata tctttttcga gcaggtttct
     3541 aacggcacga cctactgcaa ggcattcatc ctgttttgcg gcactcatgg caggagcaga
     3601 cgaactgtag ggaaggcaca tacccagagc ttcgatggca gaggccatgg tattggcagt
     3661 gtacatacca ccacaggcac ctgcaccggg gcaggcatgg cggatgatac ctttaatatc
     3721 ttcaactgtg atgtcatgat tgattttttt acccagagct tcaaatgcag agacaatatt
     3781 gagtgattgt cccttccatt gtcctgaatg tatagtgccg ccaaacacca tgagggatgg
     3841 cctgtttagc ctacccatag ccatgatggt gcccggcata ttcttgtcac atccggctac
     3901 tgccactacg gcatcatacc attgagcact gacaactgtt tctatcgagt cagcgatgat
     3961 atcacgagac accaatgaaa atcgcattcc cgcggtaccg ttcgatatgc cgtcacttac
     4021 acctatagta ttaaagatca gaccgaccag atcttcttgt aaaacgcctg attttaccga
     4081 tttagcgagg tcattaaggt gcatattgca cgtattaccc tcatacccgg tactaacaat
     4141 accgacctgt gcttttttta agtcttcttc agttaatcct atgccgtaaa gcatggcctg
     4201 agcggcaggc tgggtatcat cctgggtgac ggttctgctg tatttattga ttggttgcat
     4261 ggaaatcctt tgtttacttt tttaccttgt ttatttgatt aatgaaaaga tagattgggt
     4321 gcctaaaaag aaaaaagcct ttcccacttg gtgagaaagg cccttaggtc atacatagaa
     4381 tcagccattc tcacccggta gagcgaataa taatagtgct gataataatg atgtatatta
     4441 atcccatatg ttttatttac aaaaaaagcc ccccgaatcg tcgggaggcc tttacattta
     4501 ttttattaat tacaatactc tccctaagat cgtgttaccg caatctcaat aataatggaa
     4561 gaaataatag ttatattttt catggttttg taaaaactac ggcgcaaatg taaacaaatt
     4621 tttgaatggg caaatttttt ttctattcag ataaaaaaac aaaacttaag aagagttgta
     4681 gctgtgattt gaatcctgca tatattcaca actgacggga aaatatccat ttcaagtcaa
     4741 atatgaaaat atttcaccat atacattgat ttgtcgaaat tggctcttat atttgtaatc
     4801 acgcaatgta ccctatttta ctacccgatg atatttcgtt ccagatttgc aatctttctt
     4861 tttattttgg ctctgtgcat cattactacg ggctgtaaaa agacgaaacc ggatgaaata
     4921 cattttgata ctaagaagaa tttctgggaa tacattgaga aaggcaacga aatttatagt
     4981 aagaagtccg atctgaaggt attcggcaag gccaaaaatt attttgattc tgctatgttg
     5041 atagcggaac atctcaaaga caccatgttg cttgccgaag cgacttatgc ttatggcaag
     5101 gtctttgacg cctggaattc caatcccgct cttacactcg cttattataa aaaagcggaa
     5161 gagctctttg cgaaatcaga ttccgttgag gccgccttat atatgaaatt ccatgttgct
     5221 catgcctata atggagtaaa agatagcacc aactgtatca agcagattaa tgaaattctc
     5281 gggataatcg atacactgcc cggttctgta gccaatgaaa taaggttttt accggaagta
     5341 gcaatggagg cagccaatgc cggtgatttt gatctcgcgg agacaatatt ggataagtat
     5401 gtcaataaat ccaatttaaa aaacgatacg cttaccttaa atcacaagga tcattattat
     5461 tatactaggg cagtcattca gattcaaaag aatatttcga catccagtta tctggatact
     5521 ttggagcagg tttttcaaga ggcccgaacc accggtgaca gcatgtttta tgcatctgtc
     5581 cttagtaaat tttatgaaaa aacaggcaac ttcaagaaat ctctttatta tagtggcttg
     5641 aactatgata tgacattcag attcatcaat aagcatgagt atgtcaatct cggtcaaaag
     5701 ctggcagaaa tggagttgaa tgctttggaa caggaaaatc agatagagac caaatccttc
     5761 atcatacttt ttgtttccat cactgccttg gccttactgt cattagcctc gatatatctc
     5821 aatgtcatca tcgggcgaag tcggctcaag cacatcaggg tatccaaaga actggaagaa
     5881 tctcatgata aaatctccct gctgtataaa gagttgcacc atagaatcaa gaacaacctg
     5941 cacatgatat tcagtttgct tcaaatgcag gaaagaaaag caactgtcaa ggcaaccatc
     6001 gagaatctaa gggcagcgcg acttaggata gaaagtattg ctgttcttca tgaagaaatg
     6061 atgcgggaga catatctggt ggattttcgg gatttcattg aacgaatgat agaaatcatc
     6121 aaggattgtt tttcctatag ccatgagatc aaaaccaatc ttgatttcaa tgatgtggaa
     6181 atacctttaa agcaaagttt ttcacttgcc ctcatcatta atgagtggat taccaacagt
     6241 ataaagcatg caaatacaag tggaaaaccg ctggcaatct atatcaattt caccgaaata
     6301 gatgataaaa tcaaggttga atattgggac aatggcactg tcgatatccg catccagcca
     6361 caaaccggat ttggatctga aatcattacg ttgctagcca aacaaatagg aaagcttatg
     6421 attaccgatc agtcaaatcc ttattattat gctattctta tcaataaaaa ttaaatccat
     6481 ccttacattt gaatgatgta gtgcagataa gtgaatgaag gaagagctac taaaaatcta
     6541 caacagtaca acctatcttg ttccttcgtt agatcttgag atccggcccg gaatacaaaa
     6601 ctcaaggtta aatgaggtat tggcacatta tggtgtcaat acattctcat tcatcactgc
     6661 atacaatccc aatggcattg agacattagg caggctgaac agggaggctc ataacaggct
     6721 tattgtcgaa gtcagccgat tagggctaca atatgtggaa ggctatggtc ggggcagcaa
     6781 tggctggaag gaagaaattt cattgcttat cctcgggatc agcctggtag agtcaggaat
     6841 tctatcccaa aaattcaagc aaaaagcctt tgttttcggg caaatcaatg aggccccctc
     6901 cattatctgg tcagaacccg attagtttga atgataatgg gtctaacatg gccaaaaggc
     6961 aggggtgtat ctgaggcaaa ttgaagagta ccaaaaaatc aaaataataa tcgtttgaaa
     7021 atcaatagat atcaaaattt tttttgctaa aatggataaa atccggtgtt tggtatcatt
     7081 ttttttatct tagcacgaat ttaatataga ttaactcaca ggatatttac catcaaaaat
     7141 gagcaataac caatccggga ccatttggtc acaatttctg actctgatta ccgtattctt
     7201 tttctggggt tttgttgctg ccagcaatga tatcttaatt ccggttttca aggaaaatct
     7261 tcatctaagt cagacacaga gccagatggt ctcttttgcc ttctatacgg catatacagt
     7321 cggatccctg atttatttct ttattagtaa gatgagaggg ggtgacatat tgaacaagat
     7381 cggttataaa aacggtatcg ccataggcct tgtcatctct gcattgggca cattaatgtt
     7441 tataccggca gccaatacac attcttttaa cttgtttatt tcaggtcttt tcattgtggg
     7501 gttaggtttc tccctgcaac aaactgctgc caatccatta gctatcgtga tgggcgatcc
     7561 caagcttggt tcacagcgac taagtatggc aggaggcgta aacaattttg ggacaacaat
     7621 cggccctctt ttggtcaatt ttgccatatt tggctcgatc tatgctgcaa cagctcaggc
     7681 ggatatcacc agcataaaga ttccatatct aatcctgggt gcagcattta ttctcgttgc
     7741 attactcatc aagttttcaa aggtacccaa tgaaattgat ctggaccaga tggcagaaaa
     7801 tgaagctgaa ggcgatagtt caaaagttcg gcacctctca ggccccatga aatatcctca
     7861 actttcactc gggatgctgg ctatctttgt ttatgtggga gtggaagtat ctacagcctc
     7921 taatttacct gaattcatga agcagcatat agccggacct gatggaggag ttttcccgac
     7981 agataagatt gcgccctata tatctcttta ttgggccagc ctaatgatgg gacgatggac
     8041 agctgccagc gaagcgttcg acctgtcaat gagcatgaaa aggatgttgc ggtattttat
     8101 gccttatatc gcttttggag tctttttgtt ggtcaacaaa atcgcaaatc acgatataac
     8161 gccattttat ttctatgtat ttatcatagc ggcaatggtc gcaggcaata tcatgagtaa
     8221 aggaaatccg gccagacaac tacttatata cagttgcctg ggtataatcg ccttgattgc
     8281 aggaatgctg gctgacggct gggtaagtac atttgccttt atatcagttg gtctcttctg
     8341 ttctacactt tggccttgta tatttaccct tgctattgcc ggattgggca agcacaccaa
     8401 tgagggctca agtctactca tcatgatgat tatgggcggg gcattcgtaa gtctctttca
     8461 gggatattta tcagaccatg taacaggaat tcaatattct tatatagcag gtgtattgtg
     8521 ttttgcatat ttagcttttt atgctattcg tacaaaaaat atattgaaag cccagggaat
     8581 cgactatgat cacggagtag taagtggggg gcattaaaca atcaatctgg agaagtagat
     8641 aaatgagtag cacagaggat ctcgcgatag gcatcgacat cggtggcacc aataccaaat
     8701 tcggtatagt caaccggaat ggtaaggtat tccatcgggg tgaaataaaa acaaggaagc
     8761 atgctgaagt agctccattc attgatgaat tgtacctttc catcctgccc atgatagagt
     8821 acgccggcgg tccggcaaga atcaggggga taggagtagg agcccccaat ggaaattttt
     8881 acaatggcac gatagaatat gcgccaaatc tgagctggaa aggggttatc cccctggcaa
     8941 aaatgttcag ggaaaaattt gatatggatg taatccttac caatgacgcc aacgctgctg
     9001 cccttggcga gatgagctat ggtgctgcaa gaggaatgcg tgactttatc gttataaccc
     9061 tcggcacagg tgtcggaagc ggtatcgtag ctaatggaca tatgatatac ggacacgatg
     9121 gatttgcggg agagctgggg catactatga ttattccaga aggaagatat caccccggca
     9181 ccggcttata tggaagcctt gagtcttatg ccagcgctac aggagtcgcc agaacagccg
     9241 tggaattcct caattcaaga cctacagtag aatctctctt gcgccagtat gccaatgaag
     9301 aaattaccag caaggttgtt ttcgattgtg caagaaaagg ggactccttg tcaaaggaag
     9361 tatattctta taccggaaag atactgggga tggcacttgc taattttgct atggcgacct
     9421 ctccggaagc aatcatactc ttcggtgggt tgtgcaatgc aggcgacatg cttttcggac
     9481 cggtaacaga ctctttcgag aagcatcttt tgcctctgtt tagaaataag atcaaaattc
     9541 ttcccagtga gttgccggac gacgatgctg caatattggg tgcaagctcc ctcgtttggg
     9601 acaaggaaag aggcgggaat tgaagtaggt atccaattca gtcaaaatct aacaaggctg
     9661 ccggaaggct atgcttactg aggtctaata ggacactacg tttgccataa tttatttttc
     9721 cttaggataa agaatctaaa gccggagatc tcaaaagtca atcggatagt ctgtaatacg
     9781 actaaattca aatgaaggca tattgccaat tttaataacc atggatgtca aattggcttc
     9841 actccttcta ttcgctatta ccatattatg cctaatttgt ttgaagataa aggaagttgg
     9901 aaaaaaatta tgacaatcgg gttctgtcaa tctaaagtat aatacctggc catagaatca
     9961 cgtaggccaa taaagtgggt accctgaaat tttacattgc gagaaataat caatgtaatc
    10021 atgcaaatta ccgactaaaa gcggagaaat tttatttcaa ttgaaaacgg gtgatttcgt
    10081 tattaattca ggtgaatcat tatatcttca gaacaatgag tcagtgccga gaaatttgcg
    10141 cacttatagt accaataccc taacaaggct tacactgcct atctgctttg actaaaaatc
    10201 cgggaagaga aaaggtatgc cattcatata ttgcacaggc tatctaaata tcggttaatt
    10261 ttgttatttt cgccctttca aagaaacccc gggtataata cctcaaattt ttctgaacaa
    10321 aataaaactc gagatatgat tctgagaatt tcgtccggtc aattatataa gatgctgcaa
    10381 gtaggccaga atgccatttc gtccaagcct ccttacccga ttcttgcaga ttttttgttt
    10441 tcaatggatg gtgataagct gagtatcact tccactgacc ttgagtttac tatcatttct
    10501 gagacgacaa tcactccctc agacaccgga caatttgcat ttcctgcaga aattctcgtc
    10561 agtacattga aggaaatgcc tgaacagccc attgaattga tttttgagga agctgacaat
    10621 atgcttaaga tgatttctgc ctatggaaac tatcgttcga gcagtaacag tatcgaagat
    10681 tatccggaaa ttccggagat caggaccgaa tcacaaatca atattccggc cagtcgtttc
    10741 ctcaaagcga taagtacctg cgtatttgct acagcaacgg atgaaatgaa agccaatatg
    10801 ctcggtatcg caattaatat tcagcctgat gccgttatcg tagctgcaac agatgctcat
    10861 aagctggtaa agtacactat tttccacgac aatggtgaga atgaaagtga attagtcatt
    10921 cccaaaaaag cagtatctgt cctccgacac atcatcgaag atgatgagaa tctccagatc
    10981 aactatgaca ggaaccatat cttttttgaa tacaaaaatt acaaggtatt ctgtaaatta
    11041 atcaatcaaa aattccccaa ctacaacagc gtcattccga tcaataatga taaggatgta
    11101 ttcatcaaca ggcaggaatg gcttcagtcc ctgaaacgaa ttacgttgta tggtaaccgg
    11161 acaacctatc aaaccagttt ccatgtagaa gaaaacgaga tcaagatcaa caccatcgat
    11221 cctgattttt ccaatgaagc ttccgaagtc atccgatgtc aatatacggg agagcctttg
    11281 gacctttcct ataacgccaa atttctgatt gattcactca gcaatcttga tgccgaagag
    11341 gtcaagctct ccatatcaag acctaatcgg gctgccattc tgacaccgaa ggaagataag
    11401 cccggggaga atattttgat gcttgtgatg ccggtcatga caaagaggct aaccgaataa
    11461 tcctgaacat gaatcatcgt atcggattgt tttttggttc tttcaatcca gtacacgtcg
    11521 ggcacatgat tatagccaat cacttagtca atgagaccga tctggatcag gtttggatgg
    11581 tcgtatcgcc tcaaaatccc ttcaaagagc aaaaaaacct tgccagtgac cggttcagat
    11641 accatctggt atatcttgcc ataggtgaca acccaaaaat tatggccagt gatatagaat
    11701 tcggattgcc caggccttcc tataccatcg atacgcttgc ctatctgcat gaaaagtatc
    11761 cggaaaagga attctgtctt atcatgggcg gcgacaatgt atccacattg aacaaatgga
    11821 aaaacagcga gcttcttctg gaaagatacc ggatatatgt ctatatgaga cccggttatg
    11881 atttgggccc attggggcaa catgccaata tcatacccct ggaagctcca ttacttgaca
    11941 tttcggccac gtatatccgt caactgcttg taagcggtaa gtcagtgcgt tacctgctcc
    12001 ccgaagcagt caggatggaa ctggagaaga gcaatcctta tcgcgacctt caatagatca
    12061 aggtgaaaag ggcctacctt atactccaca ttgcagtatt tctcgccgga tttacaggta
    12121 tattcggcaa gttgatatct ctgagtcagt ttccattggt ctggtttcga gtcctttttt
    12181 ccgtactcat tctatttctt ggattgaggt tattgaagat caggatagga ggaggtaaag
    12241 cccagatatt taaaatcgca acagtgggtc aattgctcat tatccattgg gtatttttct
    12301 atggaagcat taaatacgcc aatgtgtctg ttgctgcaat ttgttatagc ctgacaagct
    12361 ttttcacagc aatctttgcg cccatcatca accgtaaaaa actacaattc ggccaattgg
    12421 cacttagcag catcactctt tccggaatcg ggttgatttt tcattttgac accaccttcc
    12481 atacaggaat cattctagga gtcatatcct cagcattcgc cgcgctttat actattacca
    12541 atgaaaaact agtgagaatt cacgacagta ggctgatcaa tctgtatcag ctttcaggcg
    12601 gggttattac cttaggctta tttctgcctt tttattacca tttttatccg gaacaggcct
    12661 ttctaccagg agggatggat gtagtctacc tgattttgtt agccgcattt tgtactgtgg
    12721 ctctttatct tttatttgcg gaagcgttga aacatctttc ggccttttcc gtcaatatgg
    12781 cattcaatct tgagcctatt tatgccatag gattagcatt tctgatattc agggaggggc
    12841 atgaagtaaa tgaatcattc tattggggtc ttgcactggt ggtgctgtcg gtgtttcttc
    12901 agaccttgat gaatttttat ggaagaagat aaaatggagg attattgcct catcgttttt
    12961 ccgaagagtt gacatcgaca aaacgaataa attgtatatc ttgtaccatt aattcaacta
    13021 aaatggatta ggatttaatt tacatccaat aagaaacaca atatgaatga tatcaattat
    13081 tttg
//
LOCUS       DAOQMC010000039        12823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1848_length_12823_cov_5.494283, whole genome shotgun sequence.
ACCESSION   DAOQMC010000039 DAOQMC010000000
VERSION     DAOQMC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1848_length_12823_cov_5.494283"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKV31_02810"
     CDS             complement(<1..687)
                     /locus_tag="PKV31_02810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HNB91814.1"
                     /translation="MGANLKEVRERIKSVISTQQITKAMKMVSAAKLRRAQQAIVQLR
                     PYSEKLNEMLSNILSNLDGDASSVYNAEREIKHVAIVIITSNRGLAGALNTNIGKEAT
                     ALARTKYAEQLDANKVTFITIGKKGYDYVKRVHKGAPVITDYINAFSDLSFSNIAPIA
                     STLMNDFATGKYDAIEISYSKFKNAGTQLFSAEQFLPVLKKPLSAMRGNMKADYIFEP
                     DQKSLLEYLVP"
     gene            complement(766..2352)
                     /gene="atpA"
                     /locus_tag="PKV31_02815"
     CDS             complement(766..2352)
                     /gene="atpA"
                     /locus_tag="PKV31_02815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNB91815.1"
                     /translation="MVNVKPDEISAILKQQLSGFHTEAELEEVGVVLSVGDGIARVYG
                     LTNAKAGELVEFETGVQAIVLNLEEDNVGVVLLGPSDDLKEGSKVKRTNTIASIQVGE
                     GYLGRVVNPLGQPIDGKGEITGDKYEMPLERKAPGVIFRQPVTEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQIGKTAIAIDTIINQKEFYQQGEPVYCIYVACGQKSSTVAQVARV
                     LEENGAMAYTTIVSASAADPAPLQFYAPFAGAAIGEFFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVISNDAIAQNMNDLPESLKKSGLV
                     RGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGVRPAINVGISVSR
                     VGGNAQIKSMKKVSGTLKLDQAQYRELEAFAKFGSDLDAATKSVLDKGVRNVEVLKQA
                     QYSPVSVEKQVAIIYLGTNNLLKDIKPSDVREFEENLFAALDKNHPEMLANFKAGKLE
                     DADLETLRSVATTVSKNFAR"
     gene            complement(2382..2924)
                     /gene="atpH"
                     /locus_tag="PKV31_02820"
     CDS             complement(2382..2924)
                     /gene="atpH"
                     /locus_tag="PKV31_02820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNB91816.1"
                     /translation="MSVSQVASRYAKSLLDLAIDQKKTEAVRGDMAVFKEALKSRDLV
                     LLLKSPIIFPDKKIAALRAVFEGKLDTLTMSFFDLTIKKGRESLLVDIADQFTEQYNT
                     LFNIALVKVTSAAPLDEATTNIILDKIKGLVGSDKQIQLETALNPDLIGGFIIQYADR
                     LYDSSVAYQLNKLKQTLTHN"
     gene            complement(2949..3461)
                     /gene="atpF"
                     /locus_tag="PKV31_02825"
     CDS             complement(2949..3461)
                     /gene="atpF"
                     /locus_tag="PKV31_02825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNB91817.1"
                     /translation="MVLLAEFNVIKPEFGLFFWTVLIFLSLWFFLGKKAFTPIANALK
                     EREDSIKDALAQADIARNEMNNLKAENEQLIAQAREEKAAMLKEAKENAAQIVSDAKS
                     KAKEEAGRILANANLEIENQKKAAISELKGQVGLLAVQVAESVIKKQLSNDPSQIELA
                     KSLANEIKMN"
     gene            complement(3544..3744)
                     /gene="atpE"
                     /locus_tag="PKV31_02830"
     CDS             complement(3544..3744)
                     /gene="atpE"
                     /locus_tag="PKV31_02830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNB91818.1"
                     /translation="MYAGIGAGLAAIGAGIGIGFIGGKGMEAIARQPEAVGDIRNNML
                     IIAAFVEAVALFAVVVAFLTAG"
     gene            complement(3825..5063)
                     /gene="atpB"
                     /locus_tag="PKV31_02835"
     CDS             complement(3825..5063)
                     /gene="atpB"
                     /locus_tag="PKV31_02835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNB91819.1"
                     /translation="MTKLYSSLNIRFSVFPRLYFLVLFTILVTPVVNFAQDHSHDAPA
                     GEVHDVVEGVLKEEEEFKMNDLIFNHINNSNDFHIFGHIAVPLPCILYSKEDGWTMFM
                     SSKLEHGHKAYNRYVLDEGIVKRLPADFPDMGHVEIGHIKHTKEGSVEVATLEHQGKE
                     YTLEKASAFTGMTSFYDFSISKVVFSMLLAAILLVILFLSISKAYRNRSGQAPRGLQN
                     LIEIIVLFIIDEVAKPMLGDRYQKHLPFILTIFFFILVCNLMGLIPFFPFSANITGNI
                     AVTMALAVMTFLVTNILGNGNYWKHIFWMPGVPPAMKLFLAPIEFLGVFIKPVSLMIR
                     LFANITAGHIIILSLIGLIFVFGKVGASMGGAIAGTALAIPFTLFLNVIEFIVAFIQA
                     FIFAILSASYIGAATEEAHH"
     gene            complement(5887..6822)
                     /locus_tag="PKV31_02840"
     CDS             complement(5887..6822)
                     /locus_tag="PKV31_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91820.1"
                     /translation="MRRNLYPPISHRPIRTDSDLSEVSSGRPFSASIKNMQVPPEFHL
                     TSSGTQKIVQMKKAGSGDTKAEAPDDKPDIYSEKYPNLDSLPVFVDREKGEKFTYSLV
                     AGKFSYAQSVLRSWLEEMAGLGADKSLLEELSLGVEDLDAYLFDFKNKKGALDEDDLI
                     YAEELSKLIIELGSTLSPKIEAERSKKKKPAPKKVKPQSSGKQILFGKASMETMKSIK
                     KVEEIGSLLEKTLVIGTTINTATSLADPAEGLYMILTLDDPFTPVAQALSDIDQIKRY
                     RIIEEISYLLALHSKELNVEERQYWEKLLASFRKK"
     gene            7513..7851
                     /gene="ssb"
                     /locus_tag="PKV31_02845"
     CDS             7513..7851
                     /gene="ssb"
                     /locus_tag="PKV31_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB91821.1"
                     /translation="MTTLSNQVQLIGNVGQQPEIKTFDNGGKIARVSLATNEKRKNAT
                     GEYITETTWHNLVFRGKHAEIVEKHVEKGKEILVEGKIINRSYEDKNGDKRYITEIQV
                     LNFKFLGGRN"
     gene            8059..8361
                     /locus_tag="PKV31_02850"
     CDS             8059..8361
                     /locus_tag="PKV31_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91822.1"
                     /translation="MIRMSSGPIVIWLSNFLCYGFFALVCRCLVIGLQTERAHYELNL
                     FQNPGKYGKRPAPSDESINPAILLIQTTRSMEAWRIKTTGVRYGLALFYPGGIGCH"
     gene            complement(8805..8878)
                     /locus_tag="PKV31_02855"
     tRNA            complement(8805..8878)
                     /locus_tag="PKV31_02855"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8842..8844),aa:Met,seq:cat)
     gene            complement(8938..9186)
                     /gene="atpC"
                     /locus_tag="PKV31_02860"
     CDS             complement(8938..9186)
                     /gene="atpC"
                     /locus_tag="PKV31_02860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNB91823.1"
                     /translation="MQLSVLTPDVEYFNGEITSVKVPGVSGEFQILNNHAPIVSALAE
                     GKVEVKTAEGKLMSFKIQGGFIEVLNNNISLLVQSITE"
     gene            complement(9220..10737)
                     /gene="atpD"
                     /locus_tag="PKV31_02865"
     CDS             complement(9220..10737)
                     /gene="atpD"
                     /locus_tag="PKV31_02865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNB91824.1"
                     /translation="MANIGRVKQIIGPVVDVSFSGEGSKLPDIFNALVIKREGTNDLI
                     LEVQQHLGEDSVRTVAMDATDGLQRGTEVIDTGGTISMPVGDHIKGRLFNVVGDAIDG
                     IGEVEKGSNAYSIHQKPPTFEQLSTETEILYTGIKVIDLVEPYQKGGKIGLFGGAGVG
                     KTVLIQELINNIAKGYDGMSVFAGVGERTREGNDLLREMIESGIVKYGDEFKHSMEEG
                     GWDLSKVDKELLKNSQATFVFGQMNEPPGARARVALSGLTFAEYFRDGDLNDPNGGRD
                     ILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKRGSITSVQ
                     AVYVPADDLTDPAPATTFAHLDATTVLDRKIASLGIYPAVDPLNSTSRILDPNVIGHE
                     HYNCAQRVKGILQRYNELQDIIAILGMEELSEDDKLVVHRARRVQRFLSQPFHVAEQF
                     TGLKGVLVPIEETIRGFNMIMDGEVDEYPENAFNLVGTIDDAIAKGKKLLAEANA"
     gene            complement(10916..11605)
                     /locus_tag="PKV31_02870"
     CDS             complement(10916..11605)
                     /locus_tag="PKV31_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNB91825.1"
                     /translation="MTPEIWISELQSQLAGHRNQANAVHIIRYLKGQFGSFGIKQGER
                     RLIVKPFIKNARIFDTQALTILLELLWQNEEREFQYTGMELLDTYRKKFGKDIKKPLE
                     QALTEKSWWDTVDFLATHTAGRSYQDGMLTTDDLYAWNSSGNIWLIRTSILTQLKYKS
                     STDWEMLQQFILPHLLHKDFFIRKGIGWALREYAKTEPARVRSFVEHHEMSGLSRREA
                     LKNLYPGGTKR"
     gene            complement(11595..12032)
                     /locus_tag="PKV31_02875"
     CDS             complement(11595..12032)
                     /locus_tag="PKV31_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNB91826.1"
                     /translation="MLAINLERARKYCAYQERCHSEVRSKLIEWKIFGEELEQIIATL
                     ISENFLNEERYARAFVSGKFNINHWGRQKITYALKQRQVSDYSIRKGLEVIDEEEYQQ
                     LIRRLLERRMKEKKTQSPYQLTQYLMSRGFEYNEIEKVLSHDA"
     gene            12196..12627
                     /gene="dut"
                     /locus_tag="PKV31_02880"
     CDS             12196..12627
                     /gene="dut"
                     /locus_tag="PKV31_02880"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNB91827.1"
                     /translation="MQVKVINKSRYSLPQYETAGSAGMDLRANTDMTLELKPLERALI
                     PTGLYIELQPGTEAQIRPRSGLSIKKGLTVINAPGTIDSDYRGEIKIPMINLSDELQT
                     IDPGERVAQMVVAKYETISWMEVEDISPTERGSGGFGSTGG"
BASE COUNT         3517 a         2907 c         2695 g         3704 t
ORIGIN      
        1 aggaaccaaa tattccaaaa ggctcttttg gtcgggttcg aaaatataat cggccttcat
       61 atttcccctc atagcgctca atggcttctt gaggaccggc aggaactgtt cagcactaaa
      121 cagctgggta ccggcatttt taaacttact ataggagatt tcgatcgcat cgtattttcc
      181 tgtagcgaaa tcattcatca gtgtcgatgc gattggagcg atattgctga atgaaagatc
      241 agagaaagca ttgatgtaat cagtaatcac cggagcaccc ttatgtactc tttttacata
      301 atcatatccc ttcttgccga tggtgataaa ggtaacctta ttggcgtcca gttgttcggc
      361 atactttgtt cgggccaatg ctgttgcctc tttgccgatg ttggtattca aggcgccggc
      421 aagccctctg ttggaggtga tgattacaat ggcgacatgt ttgatttccc tttcagcatt
      481 gtatacagat gaggcatctc catccaggtt ggaaagaata ttggacaaca tctcattgag
      541 cttttcacta taaggacgca actgcacaat ggcttgttgg gcacgacgca acttggcagc
      601 cgaaaccatt ttcatggcct tagtgatttg ttgggtcgat ataaccgact tgatacgctc
      661 tctgacttcc tttagattag cacccatgaa taagtgttat aatttataat ggtggagaga
      721 agaaacgaaa gtaaatttta tttcttccct ccgacttgat tttatttatc tggcaaaatt
      781 tttggaaaca gttgtggcga cggatctcaa tgtttcaaga tcagcatcct ccagctttcc
      841 ggctttgaaa tttgccagca tttcggggtg attcttatct aatgcagcga agagattctc
      901 ttcgaattca cgcacatcgg aaggtttgat atccttcagc aggttgttgg ttcccagata
      961 gatgattgcc acctgttttt caacacttac cggagaatat tgtgcctgct tcaacacctc
     1021 aacgttacgt acacccttgt caagaacaga tttggtggcg gcatcaagat ccgaaccaaa
     1081 cttggcaaat gcctccaact ccctgtactg cgcctggtcg agcttcaatg ttccggaaac
     1141 ctttttcata gacttgatct gagcgttacc tcctacacgg ctcacggaga ttcccacgtt
     1201 gatcgccgga cgtacacccg agttgaatag gttggactcc aggaagatct gaccgtcagt
     1261 aatggagata acgttggtcg ggatatatgc cgaaacgtca ccggcctgag tttcgatgat
     1321 cggaagagcg gtaagagaac caccgcccct gaccaggccg gatttcttta gagattccgg
     1381 caaatcattc atattttgtg cgatggcgtc gttgctgatt actttggcag cacgctccaa
     1441 aaggcggctg tgcagataga atacatcacc cggataagcc tcacgccccg gtggccttct
     1501 caacaaaaga gacacctcac ggtatgcaac cgcctgcttc gacaaatcat catagatgat
     1561 cagtgcagga cgtccggtat cccggaagaa ctcaccgatg gcagcaccag cgaaaggagc
     1621 gtagaactgt agcggagcgg gatcggcagc tgaagcagaa acaatggtag tatatgccat
     1681 ggcgccattt tcttccagga ctcttgcgac ctgtgctacg gtagaggatt tctgaccaca
     1741 ggcaacatag atacaataaa caggctctcc ttgctggtag aattcttttt gattgatgat
     1801 tgtgtcaatt gcaatagccg tctttccaat ctgacggtca ccgatgatca actcacgttg
     1861 gccacgacca atcggaatca ttgcatcaat cgcctttata cctgtctgga gcggctcagt
     1921 caccggctga cggaaaataa ctccgggagc tttacgctcc aaaggcattt catatttatc
     1981 acctgtgatt tcacccttgc catcaattgg ctggccaagc ggattgacca cacgacccag
     2041 ataaccttct cccacctgga tggaagcaat ggtattggtt ctcttgactt ttgaaccttc
     2101 tttcaggtcg tcagatggac ccagcaatac aacaccgacg ttgtcttctt caagattcaa
     2161 tacgatagcc tgaaccccgg tctcaaattc aacgagctct ccggccttgg cattagtaag
     2221 tccatagaca cgggcgatac cgtcacctac tgacagtact acaccaacct cttccagttc
     2281 tgcttcggta tgaaaaccgg aaagttgctg cttcaggatt gctgagattt catcaggttt
     2341 aacattaacc atggttatta taagttttat tttttaaata attaattgtg ggtgagtgtt
     2401 tgcttcaatt tattcagttg gtaagccaca ctactgtcat atagtctatc cgcatactgg
     2461 atgataaatc cgcctatcag gtccgggttt aaggctgtct cgagttggat ttgcttatca
     2521 ctaccgacca gtccttttat tttgtccagg atgatgttgg tagtggcttc atcgagcggg
     2581 gccgccgagg taacctttac taaggcgata ttaaacagag tattatactg ttcggtaaat
     2641 tggtctgcaa tatctaccag caatgattcc cggcccttct tgatggtcag atcgaagaat
     2701 gacatcgtca aggtatctag ttttccctcg aagacagctc tcaacgctgc aatcttcttg
     2761 tcggggaaaa tgatagggct tttgagcagg aggacaaggt ctctgctttt caaggcttct
     2821 ttgaatactg ccatgtcacc gcgcacagct tcggtctttt tctggtctat cgccagatca
     2881 agcaacgact ttgcgtaacg cgatgcaact tgtgatacag acatttataa tgattttcct
     2941 tggatgtatt aattcatttt aatctcattc gctaatgact tcgccagttc aatctgagat
     3001 gggtcattgc tcaattgctt cttgatcaca ctttctgcta cctgtacagc cagcaagcca
     3061 acctggcctt tcagttcaga gatggcggct tttttctgat tctcgatttc gagattggca
     3121 ttggctaaga tacgaccggc ctcttccttg gcttttgatt tggcatcaga gacgatctgt
     3181 gcagcatttt ctttagcctc tttaagcatg gccgcttttt cttcacgagc ctgggctatt
     3241 aactgctcat tttcagcctt cagattgttc atttcgttcc tggcgatatc tgcctgcgcc
     3301 agcgcgtcct tgatggagtc ttcacgctcc ttcaaagcgt tggcaatagg agtaaatgcc
     3361 tttttaccca ggaagaacca aagggataag aaaatcaaaa cagtccagaa aaacaatcca
     3421 aactcgggct tgatcacatt gaattcggct aataaaacca tattattttg attgaaattt
     3481 ttttaaaata agcccgtcat taaaccaatc gtcttcacga cgggcagatg ttttaggatt
     3541 taattatcct gctgtaagga atgcaacaac cactgcgaaa agcgcaaccg cctcaacgaa
     3601 ggctgcaatg atcaacatgt tgttacggat atcaccaaca gcttcaggct ggcgggcaat
     3661 cgcctccata cctttaccac cgataaatcc aattccgata ccagcaccga tagccgccaa
     3721 tccggctcca attcctgcgt acataatgta attatttaaa ttgtttgtta ttcaattaaa
     3781 atttcaaatt cgggcaagaa aacttttttc ctcgcttttt ataatcagtg atgtgcctct
     3841 tctgtcgctg ctccgatgta ggatgcagaa agtatcgcga aaataaacgc ttgaataaag
     3901 gcaacaataa attctattac gtttagaaag agcgtaaatg gtattgcgag tgccgttccg
     3961 gcaatggctc cacccatact tgctccgact tttccaaaca caaaaatcaa tcctattaaa
     4021 ctcagaatta tgatgtgtcc tgccgtgatg ttggcaaaaa gtcgtatcat taaagaaacc
     4081 ggcttgatga atacaccaag aaactcaatc ggtgccagga aaagcttcat cgccggaggc
     4141 acgcccggca tccagaagat atgcttccag tagttaccgt tacccaggat gttggtgacc
     4201 agaaaggtca tcacggctaa agccatggtc acggctatgt ttccggtgat attggcactg
     4261 aatgggaaga atggaatcag tcccatcagg ttacatacaa ggatgaagaa gaaaatagtc
     4321 aggataaagg gaaggtgttt ctggtatctg tctccgagca ttggcttggc gacctcatcg
     4381 atgataaaga gaacgataat ctcgatcagg ttctgcaaac ccctgggggc ttgtccggat
     4441 ctgtttctat atgccttgga gatggataaa aacaggatga caagcaagat tgccgccaaa
     4501 agcattgaaa agacaacttt tgatatagag aaatcataga atgaagtcat gccggtaaag
     4561 gcagaggctt tttctagcgt gtattccttt ccctgatgtt ccagtgttgc tacctctacg
     4621 gatccctcct tggtatgttt gatgtggccg atctctacat gacccatgtc aggaaagtcg
     4681 gctggaagcc tttttacaat tccttcatcc agcacataac ggttataggc cttatgtccg
     4741 tgctccaact tagacgacat aaacattgtc cagccatctt ctttggaata aaggatacat
     4801 ggcagcggca ctgctatgtg accaaagatg tggaagtcgt tggaattgtt gatgtggttg
     4861 aagatcaggt cgttcatctt gaattcttcc tcttctttga ggacaccttc cactacatca
     4921 tgtacttccc ctgccggagc atcatggcta tgatcttggg caaaattcac caccggtgta
     4981 accaggattg tgaaaagcac taagaaatac agccggggaa agactgaaaa gcgaatattg
     5041 agagatgaat acaactttgt catatgcgtt aaattgctta gcctgcaagg gttccatgca
     5101 ggtttttcaa aattcggcac aaaggtaagc tgtgagtcaa atataaccaa ggattttacc
     5161 ccctcttttt tagaaaatca atagtttata aacataagtt gattcacggt ttgttgtcct
     5221 gctctataaa aaaatatccg gttgttttta ttccggggac accattttcc cgtctaagga
     5281 aactccacag accatcgaaa aatcaggagc tatctacacc aaattgattc tgatcgttct
     5341 gtcagccatc cggattatag ccatcccgat gtacttttac ttttaaacaa agtgtaatcg
     5401 cttttcccac gtcaaccccg cttccttaaa aactattgcc tgcgtcggac ttgcaacaga
     5461 atacagtaat ttttcctgga atgatacatc taccccaaag gctttaagac aaaagcagcc
     5521 gggaatttga ttttcaaaag atacaggaag cgattggatt tgaaatatcg acgtatcaaa
     5581 aaaatctgta gtacacaaaa taaacattta tcagcggaat gcccctgttt acgcaggatc
     5641 agatctgcat taacgcaatg tgggtcattc aggtggcttt aaaatggaat gttctatagc
     5701 ccatactatt ttacgtaagt ttaaaagcag agtaggatat cattgatttt tttcaggaat
     5761 tcaggtgacc aaagatattt ctgcaatctc atcatatttt cctgttatcg gtggtaataa
     5821 gagatgcttt cttgaattca ggtcggatca acttcatgga atcatatatc acaccaggct
     5881 ataaccctat ttttttctaa aacttgctaa aagtttctcc caatattgcc tttcttcaac
     5941 atttaattct ttagaatgta atgcaagtaa ataactaatt tcctcgatga tgcgatacct
     6001 ctttatttga tcaatatctg ataatgcttg agcaacagga gtgaagggat catcaagtgt
     6061 gagtatcatg tagagacctt cagccgggtc cgctaatgaa gttgccgtat tgattgttgt
     6121 accaataacc aatgtttttt ccagaagact accaatttct tctacttttt taattgattt
     6181 cattgtttcc atagaagcct tgccaaataa aatctgtttg ccggaggatt gaggcttaac
     6241 tttttttggc gccggtttct tttttttgct tctctctgct tctatttttg gggaaagggt
     6301 tgaacctaat tcaataatta atttagaaag ctcttcagca tagatcaaat catcctcatc
     6361 gagggccccc tttttattct taaaatcaaa gagataagca tctaagtcct caacaccgag
     6421 agacaactct tccaatagtg atttgtctgc acccagaccc gccatttctt ctaaccaaga
     6481 acgcagtact gactgtgcat aactaaattt accggccaca agactatatg taaatttttc
     6541 acctttttcc cggtcaacaa aaacgggtaa tgagtctaaa tttgggtact tctctgagta
     6601 aatatcgggt ttatcatctg gcgcttctgc tttggtgtca cccgatccgg cttttttcat
     6661 ttggacaatt ttctgcgtgc ctgaagaagt taaatgaaat tccggtggca cctgcatatt
     6721 tttgatagat gccgagaagg gccgcccact cgaaacttct gacaaatcag aatccgttcg
     6781 aatcggacga tgacttatcg gtggatacag atttcggcgc ataataattg aaaattttta
     6841 ttcagaaaat ctacaaagcc ggataataaa aacaaaagag attctctcag tgtggcattt
     6901 aacgagcctt caaaaataca aaataatgct ggtattaatt tatttgttta attcctattt
     6961 aatttaggaa agtgctgccc aacagtcgct tgaaccttct ttctccttac cgatgtctcc
     7021 caacttagag gtaaggcact tgcacaaaag ctcaaagcga atcacgattg ccctataagc
     7081 atgtatagtc ccccaaaagt caggtgcttt tacgaagaca caatttttac cttaggatgc
     7141 gaagccggaa aattaaagaa aggagtacgg caggctcgtt gatgtctata tttttcataa
     7201 tgacacaatc gaaatccgat ttgtaagagg tttattattt cgaaattagt tataaaatac
     7261 aacttcgatt tccggtttgt cattcgcctc aaacgcctat aaacaggggc tttctaccat
     7321 tctttacccg tttgtaagcg cttacagtcg tttacaaacg actacaaacg tttatttacc
     7381 ggataaatat tgacatgtgt ttcatctttg ccttgtcaat cggaatgaat gacttttggg
     7441 aataaatccg ggcatcaatt cattgcgaat aaacttttaa ttcatatcat aaacttttta
     7501 aatcattaca ttatgacaac gctaagcaac caagtccaac tgatcggaaa cgtaggtcag
     7561 caaccggaaa tcaaaacttt tgacaatggt ggaaaaatcg cacgggtaag tctggcaacc
     7621 aatgagaagc gcaagaatgc gacgggtgag tatatcacgg agaccacctg gcacaatctc
     7681 gtattccggg gaaaacacgc agaaattgtg gaaaagcacg tcgaaaaagg taaggaaatc
     7741 ctggtagaag gaaagatcat taatcgttcg tatgaagaca aaaatggtga taagcggtat
     7801 ataaccgaaa tccaggtcct taacttcaag tttttgggag ggcgtaatta gatcggggcc
     7861 ggtgtcggct tggttaatct gactgagtca cacctttccc gtcttggcat caatgcctga
     7921 aatcatctac aacctgactg tttccggtgt tgatgccttt cgatatcaat gacaatgctt
     7981 tggattggat ggccgggcgg atgaggcact attgtttggt aaaacacaga accgcttgtc
     8041 tgctgatacc attgcctttt gataagaatg tcatccgggc caattgtaat ttggttatcc
     8101 aactttctct gttatggctt ctttgctctg gtgtgccggt gcctggtcat cggcctccaa
     8161 acagaaaggg cacactacga attgaatttg tttcaaaatc ccggtaaata tgggaaacga
     8221 cctgccccaa gtgatgaaag cattaacccg gctatacttt tgattcagac aacgaggtct
     8281 atggaagcat ggaggataaa gacaaccggt gtacggtatg gtttggcctt gttttacccg
     8341 ggagggattg gttgtcattg acggtcaaga gcacaacaca gcttcaaatc agcaatcttc
     8401 aggtagtgtg gtaatgttcg aaaaaggtaa tgttcaaaaa aggtaaggga atgttcaaaa
     8461 aagcaactgt ataccaggat gattcatata atagtccata aaatctagga atactagtcc
     8521 cgggatgaaa caatccgggg ttcagatata cttttccgga ttgctatgct actagagcgc
     8581 tgctctgtcc cgggagtgaa acaatccggg gttccgggat accgaggtta cagtcgtata
     8641 gaccaatttc attgatttat aacgaatgtg ccttcggttt tatcttcaat ttgcgttgaa
     8701 acgtgttgag tttgttaaat ttgggttaaa atcgtgttga aatcgtgttg aaatcacaca
     8761 aaataaaaaa gggcttcatt gctgaaaccc ttatcatcat tgagtagcga ggggggggct
     8821 tgaacccccg accttatgat tatgaatcat acgctctaac cagctgagct acctcgccat
     8881 ggtttggggc ggtgaagata aattcatttt ttcaaaccaa aagctttttc tggtttttta
     8941 ttctgtaata ctctgtacca ggagagaaat attgttgtta agaacttcaa taaagcctcc
     9001 ttgtatttta aatgacatca gcttgccttc tgcggtcttt acttctacct tgccttcggc
     9061 aagtgctgac acaattggcg cgtgattatt cagtatctga aattcaccgg acacaccggg
     9121 caccttgact gaagtaatct caccgttgaa atattcaacg tcaggggtca atactgatag
     9181 ttgcatcttc cgatttttat tatttcttgt tcctctctgt tatgcattag cttctgcaag
     9241 caacttttta cccttggcaa tggcatcatc gatggttcct accaggttga aggcattctc
     9301 aggatattca tccacctctc cgtccatgat catattgaat ccgcggatag tctcctcaat
     9361 tggaaccaaa acgcccttaa gtccggtaaa ctgttcggcc acatggaatg gctgagacaa
     9421 gaatcgctgt accctacgag cacggtgtac caccagtttg tcatcttctg aaagctcttc
     9481 cataccaagg atggcgatga tatcctgcaa ttcattataa cgttgtagaa taccctttac
     9541 cctttgtgca caattataat gctcatgacc gataacattc gggtcaagga tgcgggatgt
     9601 cgagttcagt ggatctacag ccgggtagat accaagtgaa gcaatcttcc tgtccaatac
     9661 cgttgtcgca tcaaggtgag caaaggttgt tgccggagcc gggtcggtca agtcgtcagc
     9721 aggcacatag accgcctgta cggatgtaat ggacccccgc ttggtagaag taattctttc
     9781 ctgcataaga cccatttcag tagccagtgt tggctgataa cctaccgctg aaggcatacg
     9841 tccaagtagt gcggatacct ctgaacccgc ctgcgtaaat cggaatatat tgtcgatgaa
     9901 gaagaggatg tcccttcctc cgtttggatc attaaggtct ccgtcgcgga aatactctgc
     9961 aaaggtcaat cctgacaatg caacacgcgc acgtgctccc ggcggctcgt tcatctgacc
    10021 aaacacgaat gtcgcctgac tgtttttaag caactcttta tccaccttgc tgaggtccca
    10081 tcctccttct tccatcgaat gcttaaattc gtcgccatat tttacgatgc ctgactctat
    10141 catttcacgc aacaggtcat ttccttccct tgtacgctct ccaacccccg caaatacaga
    10201 cataccgtca tatcctttcg caatattgtt aatcagctcc tgaatcaata ccgtctttcc
    10261 tacaccggca cctccgaaca aaccgatctt accacccttc tggtatggtt cgacaaggtc
    10321 gataaccttg ataccggtat aaagaatctc cgtctctgta ctcaattgct caaaagtcgg
    10381 tggtttctga tggatggagt aagcattgct gcctttctct acttcaccga taccatcgat
    10441 cgcgtcgccc acgacattaa ataagcggcc tttgatatgg tcgcccaccg gcatggagat
    10501 ggtgccaccg gtatcgatga cttcagtacc ccgctgcaag ccgtcggtag cgtccatcgc
    10561 tacagtacgc acactgtctt ctcccagatg ttgctgaact tcaaggataa ggtcattggt
    10621 gccttctctc ttgataacca atgcattaaa gatatccgga agcttagagc cttctccgga
    10681 aaagctaacg tcaactacag gaccaatgat ttgtttaacg cgacctatgt ttgccatgtt
    10741 tttttcttga taaatttagt aaaatcaatg gtttaagtgg ccttgcccca aaaacggtgc
    10801 aaaaatataa caaccaacgc acctaaccaa aaagttttca ctatttttat aaattactgt
    10861 aagatagaac aatgggtctt gacaaatttc tgtcattaca gcttcctgct atgcttcaac
    10921 gttttgttcc gcccggatac aaatttttca atgcttcccg tcgactcaat cccgacattt
    10981 catgatgctc cacaaatgac cgaacacgtg caggttccgt cttggcatat tcccgcaagg
    11041 cccagccaat gccttttcgg ataaagaaat ccttgtgcaa gaggtgaggc agaataaatt
    11101 gctgaagcat ttcccagtca gtggaagact tgtatttcag ttgcgtgagg attgaagtcc
    11161 tgatcagcca gatatttccg gaggaattcc acgcatacag atcgtccgta gtgagcatcc
    11221 cgtcttgata ggatctgcct gcagtatgtg tggcaagaaa atctacggtg tcccaccagg
    11281 acttttcggt gagtgcttgt tctaatggtt ttttgatgtc ttttccgaat ttttttcggt
    11341 acgtatccaa cagttccatc cccgtatatt gaaactccct ttcttcgttt tgccaaagta
    11401 attcaagaag gatggttaat gcttgtgtgt caaaaatacg ggcattctta ataaagggtt
    11461 tcacgataag acgccgttct ccctgcttga taccaaatga cccgaattgt cccttcaggt
    11521 atctgatgat atgcacggca ttggcttggt tgcgatggcc ggccagctgt gattgcagtt
    11581 cggaaatcca gatttcaggc gtcatggctt agtacttttt cgatttcatt gtattcaaat
    11641 ccacgggaca taagatattg tgtcagctgg tagggagact gggttttctt ttccttcatt
    11701 ctacgctcca ggagtcgtct gatgagctgc tgatattctt cttcatctat aacttccaat
    11761 cccttgcgga tactgtaatc ggaaacctgc ctttgcttca aggcataggt gatcttctga
    11821 cgcccccaat gattgatgtt aaacttgccc gaaacaaagg cgcgggcata ccgttcttca
    11881 ttcaggaagt tctcactaat cagggtggca atgatttgtt ccagttcttc tccaaaaatc
    11941 ttccactcaa tcagcttgct gcgcacctcg gagtggcatc tctcctgata agcacaatat
    12001 tttctggccc tttccaaatt gatcgctaac attatgcaaa ggtaatcggg aaaaggtaag
    12061 aaaggattga ttgatgtgta tatttaattg gattaaacac gacaaccatc ctcccgacac
    12121 aactatcttc caattattag gtattaatga cggaaatgtt atcttcgcat actttacagc
    12181 aaaaattttt ataccatgca ggtcaaagta atcaacaagt cgcgatattc tcttcctcag
    12241 tacgagactg ccggtagcgc cggcatggat ttacgggcca atacggatat gactcttgaa
    12301 ctaaaacccc tggaacgcgc ccttatcccc accggattat acatcgaact tcaacccggt
    12361 acagaagctc agataaggcc ccgaagcgga ctttccatca aaaaaggact gaccgtaatc
    12421 aatgcgccgg ggacaatcga cagtgattat cgcggcgaaa tcaaaattcc tatgatcaat
    12481 ctgtccgatg aacttcagac catcgatccg ggagagcggg ttgcacaaat ggttgtggca
    12541 aaatacgaaa ccatctcgtg gatggaagtc gaagatatca gccctacgga acgcggctcc
    12601 ggcggattcg gttccaccgg ggggtaatcc atggacaaaa gctaaaggaa ggagagcatc
    12661 cggatgattg aaaagtccgg cgcccaagac agagatcaaa tccctgcgat aatatgtggc
    12721 ggcgcaatct tgtcaataaa atccctccaa caggagttct ttatttctca acatcatatt
    12781 cgctgcgata atatgtggcg gcgcaatctt gtcaataaaa tcc
//
LOCUS       DAOQMC010000040        12816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1850_length_12816_cov_4.847426, whole genome shotgun sequence.
ACCESSION   DAOQMC010000040 DAOQMC010000000
VERSION     DAOQMC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1850_length_12816_cov_4.847426"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1980
                     /locus_tag="PKV31_02885"
     CDS             202..1980
                     /locus_tag="PKV31_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNB91828.1"
                     /translation="MGYFRSQLKKFLVWRIKNIPERQFVLLISVVIGFVSGLGAVLIK
                     NITYEIKWFIQTEFVQNYFTILYFVFPAIGLTLTYLASKYILGRITGQGISDTLYAIS
                     KKNGKMGIRGMYSPLVAVPMTVGFGGSVGLEGATVFMGSSIGSNLARLFHLNQKTIYL
                     MIGCATAGALASNFHAPIGAIIFAIEIFSLDLTIASIVPMLLASSIGALTSIWIYGDE
                     TLFKFVIQDKFAPKDIPFYILLGVVGAMTSLYFIMMYKWMGKLFAALDKQWKKLLVGS
                     LILGAAIYFFPSLYGQGYSTITPLLVGKHHVVMGNNGWYGSKDMLMVTGLMFTIVALK
                     VVATSATIGAVRVGGIFSPSLMSGCCMGYGFALLVNYSGIGQVSPTNFALVGMATQMA
                     GAMHAPLTAIFMISELTGTYELMLPLMICTAISYSINKYFNEYNVYQEKLAQQRALMT
                     HDKDKTVLIMITLDKVIETNFISITPDMTLREMLHSAVEQSTRNIYPVVNDENIFLGV
                     VTLDDIRTIMFDQSQYDVIKVSELMHSAPAVIQYSDTMEDVMEKFQTSMAWNLPVCED
                     GKYKGFVSKSRVLSVYRRKLIDIAGV"
     gene            2370..2906
                     /locus_tag="PKV31_02890"
     CDS             2370..2906
                     /locus_tag="PKV31_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNB91829.1"
                     /translation="MQDEINEQELQDNINDAMEAENVGNPEETDELSVLKGKVAELED
                     KNLRLFAEFENFRRRTQKEKLELMMTAAKDTLSALLPVLDDFDRAKKIVDDVEEKDPF
                     TEGVLLVYNKLFNVLKSKGLQVLESNGQPFDAELHDAISHIPVSDPSLSGKVIDTVER
                     GYLLNDKLIRHAKVVVGA"
     gene            2944..4101
                     /gene="dnaJ"
                     /locus_tag="PKV31_02895"
     CDS             2944..4101
                     /gene="dnaJ"
                     /locus_tag="PKV31_02895"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNB91830.1"
                     /translation="MSKRDYYEVLGVSKGADEAEIKKAYRKVAMEFHPDRNPGDKVAE
                     EKFKEAAEAYEILSNPDKKARYDRLGHAGVDPNFGGGGYGGHGMTMEDIFNNFGSIFE
                     DSPFGSFFGGGQQRRQGGGQRGSNLRIKLKMTLEEINTGVKKKIRVNKQVSCQTCGGS
                     GAKDRSSVKTCTTCNGAGAVREIRNTFLGQMQTTSTCPTCNGAGKMVTANCTSCKGEG
                     RVNAEETIEFEIPAGVSDGMQLSMAGRGNAGQKNGPAGDLVITVEEQPHEFLERDGNN
                     LLYDLNINIADAAIGTSVEVPTIDGKARIKVPSGTQSGKIFRLRGKGLPALQSYGRGD
                     QLIQVNVWTPQKLNDEEKRLLEKLRSMPNFNPDPEKQPKGFFQRMREMFEG"
     gene            4234..4707
                     /locus_tag="PKV31_02900"
     CDS             4234..4707
                     /locus_tag="PKV31_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNB91831.1"
                     /translation="MNKYLVGLLYLLPVILLTSCSGNIYNEERKIAEGIWEYNDSLRF
                     DFEIPDTAQLYTMELDVKHRDEFPYENCYVRIKSLYPDKTLKDDRLSLEFADETGMWM
                     GEKSGGSWLAPIAIQPVAKFRQPGKYSMIFFQDTRQDSLPGIESLRLKIARYKAK"
     gene            5620..6360
                     /locus_tag="PKV31_02905"
     CDS             5620..6360
                     /locus_tag="PKV31_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNB91832.1"
                     /translation="MKVLVCISKTPDTTAKISFKNDGKDFQSDGVQYIMNPYDEWFAL
                     VRGLELTEAQGGTVTVLNVGPADNDTIIRKALAIGANGAVRINKEPEGAWDVAHQIAA
                     FARTQAYDLILLGKETIDYNDSEVGATLAELLDLPFISYANKLDVNGAEAVVNREIEG
                     GTEIMQGQLPLVISCAQGMAEQRIPNMKGIMTAKSKPLNVIDPVAVDSPVTIVRHELP
                     PAKKSVTLIAPEDTDELVRLLHEEAKVI"
     gene            6407..7369
                     /locus_tag="PKV31_02910"
     CDS             6407..7369
                     /locus_tag="PKV31_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNB91833.1"
                     /translation="MSVIIFAESVEGQVKKSAFEAVTYGRKVADALGVACKAITLGKA
                     ENASELGVYGAQEVYNAGDAAFDQFDSQVFATAIADAFNQLGGKILVLSHSSMGKSLA
                     GRLSVRLNAGCASGASELPDISNGFIVGKSVYSGKASAFYNINTENKIVSLMGNAIKP
                     EKTGSPVEVKPLAIVAGVPRIKVVERKTISGTVPLPEAALVVSAGRGMKDPSNWGMIE
                     ELAGELGASTACSRPVSDTGWRPHHEHVGQTGLAIRPNLYIAIGISGAIQHLAGVNNS
                     RVIVVINKDPEAPFFKAADYGIVGDLFEVVPRLIESVRKFKAGH"
     gene            7601..8191
                     /locus_tag="PKV31_02915"
     CDS             7601..8191
                     /locus_tag="PKV31_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HNB91834.1"
                     /translation="MAAKKKIQLDIIALSHSVTQSNNYAVVLGEVKGNRRLPIVIGGY
                     EAQAIAVSMERMVPNRPLTHDLFKNTLDTFNIRIVEIVISDLIEGIFFAQLICEREGD
                     VFEVDSRTSDALAMAVRFDCPIYTFDHIMDSAGVVLEIDAEEESPAEESHHTESTYSS
                     KTTEELNQMLEDSLQEENYEKAALIRDEIKRRKGGS"
     gene            complement(8412..10868)
                     /gene="bamA"
                     /locus_tag="PKV31_02920"
     CDS             complement(8412..10868)
                     /gene="bamA"
                     /locus_tag="PKV31_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNB91835.1"
                     /translation="MAYTNRAIGQAEEKNIYEIGSLTITGAKYSDENAIKTISGLRVG
                     DEIQVPGPKITRAIRSLMNLKLFSDVVIIQEKTIGEVIFLEIRVEERPRLLGWSFSGV
                     KKSYHEDLNKKIQPYMLKGGIVTENNKSNAINAIKNFFIDKGYLDAMAKVRETSHDTT
                     LNQVKLVFNVTRGKRIKIQDVTFSGNQNVKSRKLRKQLDDTKVKRRLFASSKFIKPKY
                     EEDKTELISYYNTLGFRDAKIISDSMWREKDGDLRIHINLAEGNKYFYRNITFKGNTL
                     YTEEQLKNRLGINKGDEYNQKKLENRLRFSMDGTDVSSLYLDDGYLAFNVDPVETAVS
                     NDSIDLEMRVFEGPQFTIDKVVIKGNDRTNENVIRRELRTLPGAKFSRSDLIRSQREI
                     INLGYFNQENIGINTPVNQKAGTVDIEYTVEEKPNDQLELSAGYGGFQGLIGTLGVSF
                     NNFSIHNIRKKEAWHPLPQGDGQKLSLRAQTNGRFFQSYNASFTEPWFGGKKPTSFTV
                     GGFYTRYSNIDAQYRSIGYYSIASGTMGLGTRLKWPDDNFLVNATLNFQQNKLKDYPG
                     LFTVANGTFNNLNLRLTLARTTVADPIFPRDGSKISLTGQFTPPYSLFNGKSAADYAD
                     LPLEKRFHWLEYHKWRLDLEFYKSIIGKLVLKVSNKTGMMSFYNSYVGLSPFERFELG
                     GDGLSNQYVGIVGKDIIALRGYTVEQVREDNKVTTDGAAVFSKISMELRYPISLNPSS
                     SIYVLGFMDGGNMWNRIRDYRPFDLKRSAGLGLRVFLPMFGILGFDYGFGFDKPDLIQ
                     NGSKWTQFGKFSFVLGFEPD"
     gene            complement(11435..12061)
                     /locus_tag="PKV31_02925"
     CDS             complement(11435..12061)
                     /locus_tag="PKV31_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNB91836.1"
                     /translation="MEPISYGYNTPLNTNLLPQNGVAIYYGNIMERCEADAYFDSLLS
                     NIAWQNDEAIMFGKRIVTKRKVAWYGDLPFEYTYSRISKYALSWTPELKRLKYITEKA
                     TGETYNSCLLNLYHDGSEGMAWHSDGEPDLKKYGAIASLSFGAERKFAFKHKISGQRI
                     DMILQHGSLLLMTGETQQHWVHRLPPTKKISTSRINLTFRTIIEKIRS"
     gene            12266..>12816
                     /locus_tag="PKV31_02930"
     CDS             12266..>12816
                     /locus_tag="PKV31_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNB91837.1"
                     /translation="MKSDSFLVTGGVTLSGSIEPQGAKNESLQVVCAVLLTADTVIIR
                     NIPDILDIRLQMELLRGLGVKIEQLGPCDFSFNAADVNLSFLDSPEYLEKARRIRGSV
                     LLLGTLLGRFGKAYLPKPGGDKIGRRNLDAHFHGFEKLGAKFTYNEDAGHFEIDGSHL
                     EGAFIYMDEISVTGTGNVLMASVL"
BASE COUNT         3505 a         2457 c         2984 g         3870 t
ORIGIN      
        1 gaaacttcca atctgcgcag aatttcatcc gcataagcgc aacttgggtt aaaatagaaa
       61 ggcagtttgg gtggctcgta gtaaccggat gctgattgga attgatgggt atcattttca
      121 tgtagcatca aaatttgtaa caaatgaagg gagaatgtca tgataaaata gcatctttgt
      181 tgacaatatt tatcattccc gatgggctat ttcagaagcc aattaaaaaa attcctggtc
      241 tggcgtatca agaatatacc tgaacggcag ttcgtgcttt tgattagcgt cgtgattggc
      301 tttgtcagcg ggttgggtgc tgtattgatc aagaatatca cttatgaaat caagtggttt
      361 attcagacag aatttgtcca gaattacttt acgatactgt attttgtctt tccagccatc
      421 ggtctgacat tgacctatct ggcttccaaa tacattcttg gcagaatcac cggtcaggga
      481 atttccgata ccttgtatgc catctccaag aaaaatggca agatgggcat ccgcggcatg
      541 tattcaccac ttgtcgcagt gcctatgaca gtgggatttg gcggttcggt aggtctcgaa
      601 ggtgccaccg tcttcatggg ctcgtctatt ggatccaacc ttgccagatt gtttcacctc
      661 aaccagaaaa cgatatacct gatgatcggt tgtgctacag ccggtgccct ggcgtccaac
      721 tttcacgccc caattggtgc aatcatcttt gccatcgaga ttttttcatt ggatctgacc
      781 atagcaagta tcgttcctat gctgttggca tcgtctatcg gtgcactaac ctcaatatgg
      841 atttatgggg atgaaacgct gtttaagttt gtcattcagg ataagtttgc acctaaggac
      901 atcccgttct acatattgtt gggtgtagta ggtgccatga cgtcattgta ttttatcatg
      961 atgtacaagt ggatgggcaa actgtttgct gccttagaca aacaatggaa aaaactcctg
     1021 gtcggatcct taattctggg tgcggctatt tatttttttc catcgttata cggccagggg
     1081 tattctacca taacgccctt gctggtgggc aagcatcacg tagtcatggg aaataacggc
     1141 tggtatggtt cgaaggatat gctgatggtg acgggtctga tgtttacaat tgtggcactg
     1201 aaagttgtag caacatcagc gacgattgga gcggtccgtg tgggaggtat tttcagcccg
     1261 tcattgatgt cgggatgctg catggggtat ggctttgccc tattggtcaa ttactcaggt
     1321 atcggacagg tatcaccgac taattttgcc cttgttggga tggcgacaca gatggcaggt
     1381 gccatgcatg cgccacttac cgctatattt atgatttcag agctgacagg tacctacgaa
     1441 ttgatgttgc cactgatgat atgtactgcc atctcctaca gtatcaacaa gtatttcaat
     1501 gaatacaacg tgtatcagga gaaactggcc cagcaacgtg ccttgatgac acatgacaag
     1561 gacaagacgg tacttatcat gattacactg gataaggtaa tcgagacgaa tttcatttcg
     1621 attactcctg atatgactct tagagagatg cttcacagtg ccgttgaaca atctacacgg
     1681 aatatttatc cggtcgttaa tgatgaaaat atatttctgg gagtagtgac actcgacgac
     1741 atccgcacaa taatgtttga tcagagccag tatgacgtta taaaggtgtc agagctgatg
     1801 cacagtgccc cggctgtcat tcagtattcg gacaccatgg aggatgtcat ggagaaattt
     1861 cagaccagta tggcgtggaa cctgcctgta tgtgaagatg gtaagtacaa ggggtttgtg
     1921 tctaaatccc gggttctgtc agtctatagg agaaagttga tcgatattgc cggagtctga
     1981 tgtagggatg aaaatcaggt aaagttcttc agaaaattca atatagtgag gctttgagtg
     2041 tccaatgata gcaaaggact cgtaaaacaa tgggtagagt tgcctgattg agtgaaatta
     2101 aaaagaatga ttggggcatt gagagcggta gcctcagcgc aacataaggc ataaagggtt
     2161 gatatattga aatcagcact ttccaacggg taacttaata gattctctct ttgcccgatc
     2221 tctcctatgt cttattgaac aatcaatcat agaattataa tcgttatttc gtccgaagct
     2281 gatcaaaaga tcaacgtctg ccaatttgtc ctgaaatgac ttttggcagt gtatttgcac
     2341 ttatgtggag aaattcaata tttaagatta tgcaagatga aataaatgag caggagttgc
     2401 aagacaatat caatgatgcc atggaagcag aaaatgttgg caacccggaa gaaactgatg
     2461 agttgagcgt cttgaaagga aaggtggcag aattggagga taagaatctg cgattatttg
     2521 cagaatttga aaacttcaga cgaaggacac agaaagagaa attggagttg atgatgactg
     2581 cagcgaaaga cactttgtca gcccttttgc cggttttgga cgattttgac agggcgaaaa
     2641 agatagttga tgatgtagaa gagaaagatc cttttactga aggtgtactt cttgtataca
     2701 ataagctgtt caatgtgctg aaatcaaaag ggctgcaggt tttggaatcc aatggtcagc
     2761 cgtttgatgc tgagttgcat gatgctattt cgcatattcc cgtatcagat ccttccttgt
     2821 cagggaaggt gattgacact gtcgaaagag gatatctgtt gaatgataaa ttgatacgcc
     2881 acgccaaagt agtagtgggg gcttgatttg ctttttgatt aaacaaacta tttgattcag
     2941 actatgtcta aacgcgatta ttacgaggtt cttggtgtat cgaaaggagc agatgaggcc
     3001 gaaattaaaa aagcataccg aaaagttgcg atggagttcc acccggatcg taatccgggg
     3061 gataaggtgg ctgaagaaaa gtttaaggag gcagctgagg catatgaaat actgagcaat
     3121 ccagataaga aagccagata tgacagattg ggccatgcag gcgtggaccc taattttggt
     3181 ggtggaggct atggcggtca tgggatgacc atggaagata tttttaacaa ctttggcagt
     3241 atttttgagg actcaccttt tggttcattc tttggtgggg gccagcagcg tagacaaggc
     3301 ggcgggcaac gtggcagtaa tcttcgcatc aagctcaaaa tgacactgga ggaaatcaat
     3361 accggagtca agaaaaaaat cagggtcaac aagcaggtga gttgtcaaac ctgtggagga
     3421 tcaggagcca aagatcgttc ttctgtcaag acttgtacga cctgtaatgg tgcaggcgct
     3481 gtccgcgaga taagaaatac cttcctggga caaatgcaga ctacatcaac ctgtccgact
     3541 tgtaatggag caggaaagat ggtaaccgcc aattgtacca gttgtaaggg agaaggacga
     3601 gtgaatgctg aagagaccat tgaatttgaa atcccggcgg gtgtatcaga tggcatgcaa
     3661 ctttctatgg ccggtcgagg caatgccggt cagaagaatg gaccggccgg ggacctggtt
     3721 atcaccgttg aggaacagcc ccatgagttc ctagaaagag atggcaataa tctgttgtac
     3781 gacttgaata tcaatattgc ggatgcggca atcggcactt cggttgaagt tcctaccatc
     3841 gacggtaaag cgcggatcaa ggtgccttcc ggaacacagt ccggtaaaat atttcgtttg
     3901 aggggtaagg gccttcctgc cttacaaagc tacgggcgtg gtgatcagct gattcaggtg
     3961 aatgtctgga caccgcagaa gctcaatgat gaagagaagc gtttgctgga gaaattgcgg
     4021 tccatgccta acttcaatcc tgatccggaa aaacaaccca aaggtttttt ccagcggatg
     4081 agggaaatgt ttgaaggttg atgtgccggt gtggggttga agaaatgagg ctttcttaaa
     4141 ttttttttgc ctctacacgc tcagtctgaa tattgtaaat attttcgtat tgtaaacatg
     4201 cactaatatt gccgtattaa aactgcttgc cagatgaata aatacttagt cggactgtta
     4261 tatcttctgc ctgtcatcct gttgacatcg tgctccggga atatctataa tgaggagaga
     4321 aagattgcgg aaggcatttg ggaatacaat gattcactaa gatttgactt tgaaattcct
     4381 gacacggcac agctgtacac gatggagctg gatgtgaagc acagggatga atttccttat
     4441 gaaaattgct atgtaagaat caagtcactg tatccggaca agaccttgaa ggacgataga
     4501 ctttcactgg agtttgctga cgagacaggt atgtggatgg gcgagaagag cggcggtagt
     4561 tggcttgctc cgatagctat tcaacctgtt gccaagttcc ggcagccggg aaagtattct
     4621 atgatattct ttcaggatac aaggcaggat agccttccgg ggatcgagtc tttaaggctt
     4681 aagattgcca ggtacaaagc gaaatgatat tttaaaaatt aagagtcaaa ggattcgatc
     4741 aatccttttt tcttcccggg caggacttac aacgttttcc ttttttaaat ttcttgcaac
     4801 aatccttttt ctttttactc tggatgatgg gcaatgcaaa gtagttgttt ctcacaggca
     4861 ttccctccgt tcgaatcata ttttgttgca ttcttttttt gttgcaaatg tatatttatt
     4921 tagattaaat acaaataata tttgatgcta aattaaaatt aatacctgat cccatgaata
     4981 cttatttatt gtggattttc aatgctttac aatatatcgc cgttgataga tttggaacag
     5041 gattccggtt cattttgatt ggtaaaagcc atcgcttgga aagttgttct tgggcttaat
     5101 acttatgggt acacaataaa tttttaattc tccttgtgga acaagaccgc agtttaagca
     5161 ggcagctaag tgtctgtttg aaatggggaa tagtcgcagg ttttgcctta ctgtctgatt
     5221 tatgaagtcg tttttccaaa actgtgcctt cagtatccca ttgcttttaa aataaatgat
     5281 tccaccttgt attcatttat cgcgagcctt ttgatcgtct ttggaagcct catcagggga
     5341 gtctgatttc tagtttgcct gccctgtggt gtaagtagtc aatgaggata ttcaacgctt
     5401 caatttgttc agtgttcatc agtattttag gatttgatgc tttgtttgaa tgttttttct
     5461 ttgagtatca ataattttac ttcaatctca taggattttc gtatatttgc atcttgtgta
     5521 caaaataatg ttttgtaaaa tctttaatta ttattgattt gtaaaataga attttgtttt
     5581 cagatttatt tgcctttaaa cttaaaattt attgaatcaa tgaaggtttt agtttgtatc
     5641 agtaaaacac ccgacactac tgccaaaatc tcatttaaga acgatgggaa ggactttcag
     5701 tctgatggtg ttcaatatat catgaatcct tacgatgaat ggtttgccct ggtccgtggc
     5761 ttggaattga ctgaagctca aggtggaacg gtaacggtac ttaacgtcgg acctgccgac
     5821 aatgacacca tcatccgaaa agctttggcg ataggagcca atggtgcagt gaggatcaat
     5881 aaggagccgg aaggtgcatg ggatgtggct catcagatcg ccgcatttgc ccggactcag
     5941 gcttatgatc ttatcctttt gggtaaggag acgattgact ataacgacag tgaagttgga
     6001 gccactttgg cagaactgct tgacctgccg tttatctcat atgccaataa gctggatgtg
     6061 aatggtgccg aggccgttgt caaccgtgaa attgaaggag gaacagaaat catgcaggga
     6121 cagcttcccc ttgtcatcag ctgtgcacaa ggcatggctg agcaaaggat accgaacatg
     6181 aagggaatta tgactgccaa gagcaagccg cttaacgtaa tcgatcctgt ggctgttgat
     6241 tcgccggtga caattgtgcg acatgaattg cctccggcca agaaatcagt gacgctgatt
     6301 gctccggaag atacggacga gttggtgcgc ctgttgcatg aagaggcgaa agtcatttga
     6361 tattgggtca atctaatttt tcaaactttt aattattaaa atatatatgt cagttatcat
     6421 atttgccgaa tcggttgaag gtcaggtcaa gaaaagcgca tttgaagcag taacttatgg
     6481 ccgcaaggtg gcggatgccc ttggagttgc ttgtaaggct ataacattgg gaaaggcaga
     6541 aaatgcttca gagctgggtg tttatggggc acaagaggtt tataatgcag gagatgcagc
     6601 atttgatcag tttgattctc aggtttttgc tactgccatt gccgatgctt tcaatcaatt
     6661 gggcggcaag attttggtcc tttcacatag ctccatgggc aagtccctgg caggccgtct
     6721 ttcagtaaga ctcaatgctg gctgtgcttc aggtgccagt gaattgccgg atatatccaa
     6781 tggcttcatt gttggtaaaa gtgtatattc cggtaaagct tctgcatttt acaatattaa
     6841 tacagagaat aagattgttt ccctcatggg aaatgccatt aagccggaga aaaccggatc
     6901 tcctgtcgaa gtgaagccac tcgcaatcgt ggcaggggta cctagaatta aggttgttga
     6961 acgaaagacc attagcggta ctgttccact gccggaagct gctttagtgg tgtccgcagg
     7021 acgaggaatg aaggatccat ccaactgggg catgatcgaa gaactggccg gagaacttgg
     7081 agcctctacg gcatgttcaa gacccgtttc tgataccggc tggaggccgc atcatgagca
     7141 tgtaggacag accggattgg ctattcgtcc taatctttat atagccatcg gtatttccgg
     7201 cgcaatccag cacctggccg gtgtcaacaa ttctcgcgta atcgtcgtca tcaataagga
     7261 ccctgaagca cctttcttca aggctgctga ttatggtatt gttggagatt tgtttgaggt
     7321 tgtaccaagg ttgatcgaat ctgtcaggaa gttcaaagcc gggcattgat ttttcaaagt
     7381 tgccggaaaa aacagctgca cggcggtggt tgaaaatatt cagatatttt aaccccgggt
     7441 taattgacca ttaattcaag gtggtttttt ggaataaata cttttcattg gggcatagcg
     7501 catgttgttt tttccggtta ttatcttttt accgattccg gcagtaacta cataacgtat
     7561 aaatacgtat ttttgagcat cataactaaa caaagccctc ttggcagcaa agaagaaaat
     7621 ccagcttgat atcatagcgt tatcgcacag cgtgacccag tccaacaatt atgcagttgt
     7681 gcttggtgag gtgaaaggca acaggcggtt gcccatcgtc atcggtggct acgaagctca
     7741 ggccatagca gtatctatgg aacgtatggt tcccaatagg ccgttgaccc acgatttgtt
     7801 caagaacacg ctggatacat ttaatattcg gattgttgag atcgtcatca gtgatttgat
     7861 tgaaggcatt ttctttgctc agctgatttg tgagcgggaa ggagatgtgt ttgaggtgga
     7921 ctccagaaca tctgatgccc tggccatggc tgtgagattt gattgtccca tctacacctt
     7981 tgatcatata atggacagtg ccggagtggt gctggaaatc gatgctgaag aagaaagccc
     8041 tgctgaagaa agccaccata cagaaagcac ttattcatcc aaaacaacag aagaattgaa
     8101 tcagatgctc gaggattcct tgcaggaaga gaattatgaa aaggcagctc tgattcgtga
     8161 tgaaataaaa agacgtaaag gcggtagttg acaccattac acgcttgctt tttggaatgt
     8221 tgtaattgcg gatgttgagc actaaagagg gaatattcct ataccaaatc aagcaccacg
     8281 attttccgga ttttatcttt ctatcctgat attgacaatt tgctcaagtt tcattatcac
     8341 atgatactga acatacgaag ggtgtaaccg attggctacc tttctcgaaa agggtatatt
     8401 aatattgtag cttaatccgg ttcgaatcca agtacgaagc tgaactttcc gaactgtgtc
     8461 catttggaac cattttggat caagtccggc ttgtcgaacc cgaatccata gtcaaatcct
     8521 aagattccaa acatgggcag gaatacccta aggcccaatc ctgcagagcg cttcaagtca
     8581 aatggtctgt aatccctgat cctgttccac atatttcctc catccataaa tccaagtaca
     8641 taaatagagg aagatggatt aagcgatatc ggatatctca attccataga gatcttggag
     8701 aatactgcag ccccatcggt ggtaaccttg ttgtcttccc ttacttgttc tacggtatat
     8761 cctcgcaggg ctatgatatc ctttcccacg atgcccacat attggttgga tagtccatca
     8821 ccacccaatt caaacctctc gaatggcgat aggcctacat aggaattata gaagctcatc
     8881 attccggttt tattggaaac tttcaagaca agtttaccta tgatggattt ataaaattcc
     8941 agatcgagac gccatttatg atattcaagc cagtggaatc gcttttccag gggcaggtct
     9001 gcgtagtctg ctgcagattt gccattgaat aatgagtatg gaggcgtgaa ttgcccggta
     9061 agagatattt tagaaccatc ccgtgggaag atagggtccg caacggttgt ccttgcaagg
     9121 gtgagccgta gattcaggtt gttgaaggtt ccattagcca cagtgaaaag cccgggataa
     9181 tcttttaatt tgttttgttg gaaattgagt gttgcattga ccaggaagtt gtcatcaggc
     9241 cacttaagtc gagtgccgag acccattgtt ccggatgcta tgctgtaata tcctatactt
     9301 ctatattgcg catcaatatt ggaataacgg gtataaaatc caccaacggt gaaggatgtt
     9361 ggttttttcc caccaaacca tggttcggta aatgatgcgt tgtacgattg gaaaaaacga
     9421 ccattggttt gagctctgag agataatttc tgtccatcgc cctgtggtag tggatgccag
     9481 gcttctttct ttctgatatt atggatcgaa aaattattga agctgactcc aagagtgcct
     9541 ataagtcctt ggaagcctcc gtatccggcg gataattcca gctgatcatt gggcttttct
     9601 tccacggtgt attcaatatc tacggttccg gctttttggt tgaccggcgt attgataccg
     9661 atattttcct gattgaagta tccaagatta atgatttcac gctgagaacg aatcaggtcg
     9721 cttcggctga atttggcgcc cggaagtgtt ctaagctctc gccgaatcac attctcattg
     9781 gttcggtcgt tgcccttgat tactacctta tcaatagtaa attgaggtcc ttcaaacacc
     9841 cgcatctcaa ggtcaataga atcgttggag acagccgttt caaccggatc tacattgaag
     9901 gcgagatatc catcgtccaa gtacaaggaa ctgacgtctg tcccgtccat actgaatcgt
     9961 agcctgtttt ccagcttttt ctgattatat tcatcgccct tattgattcc caggcggttt
    10021 ttcaattgct cttcggtgta tagtgtattt cctttgaaag taatgtttct gtaaaaatat
    10081 ttattacctt cagccagatt gatgtggata cgcaggtcgc catccttctc tctccacata
    10141 gaatctgaaa ttatcttcgc atcccgaaat ccgagtgtgt tgtaatagga aatcaactct
    10201 gttttgtctt cttcatattt tggtttgata aatttagaag aggcaaacag acggcgcttg
    10261 acctttgtat catcaagctg tttgcgaagt ttacgtgatt ttacattttg attgccactg
    10321 aaggttacat cctgaatctt gatgcgtttg cccctggtta cattgaatac taatttgacc
    10381 tggttgagcg tcgtgtcgtg acttgtctct cttactttag ccattgcatc aagataacct
    10441 ttgtcgataa agaaattctt aatggcattg atggcgttgc ttttattatt ttctgtaacg
    10501 atgcctcctt tcaacatata aggctgaatc tttttattca gatcttcatg ataggatttc
    10561 ttaacaccgg aaaaagacca tcccaacagc ctgggccttt cttctaccct tatctcaagg
    10621 aaaataactt ccccaatggt tttttcctgg atgatgacta catcggaaaa taatttcagg
    10681 ttcatcaaag atcggattgc cctggttatc ttgggcccgg gaacctgaat ttcgtcgcct
    10741 acccgaagtc ctgaaatggt tttgattgca ttttcatcac tgtatttcgc tccagttata
    10801 gttaaggagc cgatttcata aatgtttttt tcctcagctt ggccaatggc acgattggta
    10861 taagccacaa gcccaattat cagaatggct atagttaaaa ttctaatatt taaatgcatc
    10921 gcaatcacaa agttatagtg tataatcagc taaattttcc atttttgtcg gaagttcaac
    10981 gagctgaata tcagtttttt gaggcgcaaa tttagagtat tattggctac tgacctaaca
    11041 ttcttcaatg aaaccttttt caataggaat aataatctga aaatcacaag tcaaacgttg
    11101 tttttccgga aaaattgtat ggcttacagg cttgtattct atgttctagg atggttcatt
    11161 catatcgttg tgatatttct ttaaccatcc aaacaattcc gataaatgga aaagcaattc
    11221 atgtatcaat tggattaaaa tgagttcctt ttattatcaa acgtcaaacc tcccggatgc
    11281 gctgccatcc ggttgttgaa tctgaatttt tgtaattatt ggactaaact aattgttagt
    11341 caggactttt tattggtaaa atatgattta ttgccttcag ggaagagcca ttatagtcaa
    11401 aatatttctt gggcgggaga ttctggctta tatttcatga tctgattttc tctataattg
    11461 tcctgaatgt cagattgatc cttgaggtgc tgattttctt tgtcggtggt aagcggtgca
    11521 cccaatgttg ctgcgtctct ccggtcatca acaacagact gccatgttga agaatcatat
    11581 ctattctttg ccctgaaatt ttgtgtttga aagcaaattt tctttcagct ccaaagctga
    11641 gggaggctat agctccgtat ttcttcagat ccggttcgcc atcactatgc caggccatgc
    11701 cttcagaacc gtcatgataa agattgagca ggcatgagtt gtaagtttct ccggttgcct
    11761 tctcagtgat atattttagt ctttttaatt caggtgtcca tgacaacgca tatttactga
    11821 tacgagaata ggtgtattca aagggaaggt caccatacca cgctactttt cttttagtga
    11881 ctattctttt gccaaacatg atggcttcgt cattttgcca ggctatgttg gatagcaagg
    11941 agtcaaaata cgcgtctgcc tcacatctct ccatgatgtt tccgtagtat atcgccaccc
    12001 cattttgtgg aagcaggttg gtattcaatg gtgtattata tccgtaagaa attggttcca
    12061 tatcctttta gcctgatgta aatataagta caaaagaatg attatcgcgg taagaataca
    12121 tggtcattcc attgaatgcg tatgtagttt tcgaaaagtt cattggttat gagattaaac
    12181 agactactgt attgtacgtt tgaatatctt ttttcagtaa aatttctata ttaggcttag
    12241 ctttgaacct caatgttaga gaaagatgaa aagtgacagt tttttagtta ccggaggtgt
    12301 aaccctatca ggtagtatag agcctcaggg cgccaagaat gaatcgttac aggtggtatg
    12361 tgctgtgttg ctgactgcgg ataccgtaat tatcaggaat attccggata ttcttgatat
    12421 caggcttcag atggagttgc ttcgaggctt gggtgtcaag attgagcagc tgggtccctg
    12481 tgacttttca ttcaatgctg ctgatgtcaa tttgagtttc ctggactctc cggaatattt
    12541 ggagaaggca aggcgtatca gaggatcggt ccttttgttg gggactttac ttggacgatt
    12601 tggcaaggcc tacctaccga aaccgggggg cgacaaaatt ggcagaagaa atcttgacgc
    12661 tcattttcat ggatttgaaa aactgggggc gaaatttacc tataatgaag atgccggcca
    12721 ctttgagatc gatggtagtc acctggaagg agcctttatc tatatggatg agatttctgt
    12781 gacaggaaca ggaaatgtcc tgatggcatc tgttct
//
LOCUS       DAOQMC010000041        12745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1865_length_12745_cov_4.816233, whole genome shotgun sequence.
ACCESSION   DAOQMC010000041 DAOQMC010000000
VERSION     DAOQMC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12745
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1865_length_12745_cov_4.816233"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKV31_02935"
     CDS             <1..271
                     /locus_tag="PKV31_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNB91838.1"
                     /translation="VANETKLFSLLANIGDTKSLIIHPASTTHQQLSEEDQGASGVTP
                     DLLRISVGLENIEDLKADLLQAFQAIYSNSHEKVEEYINQELSLT"
     gene            225..1181
                     /locus_tag="PKV31_02940"
     CDS             225..1181
                     /locus_tag="PKV31_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB91839.1"
                     /translation="MKKLKNILIRNFPLPDGSKESFKVSYQIFGQELHTAPIILINHA
                     LTGNSSVAGEDGWWKEVVGDSKAIDLRRYTVIAFDIPGNGYNHVAPIERYTLLTTKIV
                     GNLFWKALDSLGVVQLYAILGGSLGGSVAWEMLFQRPDSIQKLIPIACTLKSSDWLIG
                     NAHVQESILSNSQNPLEDARKHAMLLYRTPNSFRAKFKGRQSESGFAVEHWLIHHAEQ
                     LKSRYTLPSYRLMNHLLKTIGENRAKDEVEKIFRNCKTEIHTIAVDSDYMFTREEQIE
                     TYKIIRKYKKNASYGEIKSLHGHDAFLIEFQQLNKLLISILS"
     gene            1178..2434
                     /locus_tag="PKV31_02945"
     CDS             1178..2434
                     /locus_tag="PKV31_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91840.1"
                     /translation="MKVINLNQSSSIQQEHRQVTQIKNFGTTSSINISSDARKPLRFD
                     SARQADNRHCQVIKTINIAIFGTGKVGGTLIDQILASGINIEKSKSIRLNIFAVGNSQ
                     KLHLDKSGISENWKFYLNKYGKHYDIWDIITYAQVHQLDNLIAVDNTASEEFTENYHT
                     LVRNGFDLVSSNKIGNSGSYEFYRSLRQILRKNEREFLYEANVGAGLPLIDTIRMLHI
                     SGDKIHRIRGVFSGSLSYIFNTFSSRDIPFHQVLHEAASKGLTEPDPREDLSGNDVCR
                     KLLILARELGLSQEFESIDNHNLIPEELGKLDLDSFQKNSHELDRHFEYLKQNVKPGH
                     VLRHIGELSINRQSCEVLLKAGLSEIPEYSALGQLTGSDSLFEIYTEGYGLNPIIILG
                     AGAGPAVTARGVFSDILRLAGRVMKN"
     gene            complement(2987..4255)
                     /gene="tyrS"
                     /locus_tag="PKV31_02950"
     CDS             complement(2987..4255)
                     /gene="tyrS"
                     /locus_tag="PKV31_02950"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNB91841.1"
                     /translation="MNFIEELKWRNMLMDYTPGIEEIFDDGPIVGYIGFDPTAPSLTI
                     GNMVQVMLLSHFQRTGNKPIVLMGGATGMIGDPSFKDAERTLKTIEEIQVNIDRQMKQ
                     FRKLLDFDGPNAAVFVNNYDFYKDMNVLTFLRDVGKTLTVNYMASKESVKKRMETGIS
                     YTEFSYQLLQGYDYQVLYDRYNCRVQMGGSDQWGNITAGTEFIRRNVGGKAYAATTPL
                     LTKSDGTKFGKSESGNIWLDPGMTNAYQFYQFWINCSDEDMPKLLRYFSLKNKKEIEE
                     LDLMLVDNPNEVKRILAEEITTRVHSAEDLDAVKKVSSLLFGKGFDEQAVKSMGTNTI
                     ELLKGELEVRSGSADQWSQGASVIDLLTELNIFTSKGEAKRAIQGQAVQINRVRITDP
                     AAISEPEQIFGNGYVFVENGKKQKVLIELA"
     gene            4367..5659
                     /locus_tag="PKV31_02955"
     CDS             4367..5659
                     /locus_tag="PKV31_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB91842.1"
                     /translation="MHLSFFIADRINRHENSAFASFIMKLAIVSVALSVSVMIITTCL
                     VMGFKNQIKSKVFDFWGHIHLNLPEYNQAIDPKPLKLNNSLLDSIRDIKSVPFIKEKS
                     FLGIPFGNVEKESKTGIQHVQGFVQLPGILTSDNQIEGLVLKGVNRDFDTSFLAKYLV
                     SGNGDIKDDSTGRTLIISQTTASRLKLNPGEDIVVYFVVGGQQIGRKFKISGVYKTGL
                     EEYDRKFAICSSTVIQKLLNWGPDEYTNYEIFVQNLNDLEAINNYLYSDVLPTNVFST
                     TIHNKFPGIFEWLQLQDYNEVVILGLMIAVCLINMATVLIIFILNRLKMIGILKTLGM
                     KNRDLRMIFLLQASRIILYGLIWGNLIGLGLSWIQYHYKPIKLNEADYYLSIAPIEFS
                     FPAIMTINIVTLTIIMLFLILPSAIVARISPVRTIEYN"
     gene            5832..6584
                     /locus_tag="PKV31_02960"
     CDS             5832..6584
                     /locus_tag="PKV31_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91843.1"
                     /translation="MTISIIVRKAGFYFTTLHIRILRLFRHIFFLIKLTFRPGEISVH
                     YSYRNRWLTWLLELPFYVIDILAIPELMEIFLQFLHPKNRLLTEREKKLINEVYGGCL
                     KLSVISFDRYASIMRDRAMAFVGVNTIFFNRELSDALLLHEVCHCLQYQKFGSIYIIR
                     ALMAQNSKEGYSYGGSDFLHEWNITSDLRKLNYEQMAEIIGHFHLISLRQINDEDDVR
                     LRKLEIYRNAAGNIINAINYQGSISSFFRLRI"
     gene            complement(6545..7612)
                     /locus_tag="PKV31_02965"
     CDS             complement(6545..7612)
                     /locus_tag="PKV31_02965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB91844.1"
                     /translation="MEQLREIDENESGQGDELFVHFALTVDKGQTPIRIDLFLLDRIM
                     NITRNKVQNVIRSGGVLVNDKPVKPNYKVRPNDNIVIVLPEPPGEYAVEPEDIPLNIV
                     YEDDDVMVINKPAGMVVHPGLGNKTGTLVNALAHYFKFDLPIKEGNIADRPGLVHRID
                     KDTTGLMVIAKTEYAMSHLAKQFFDHTVRREYTALVWGELDPLAGRIVGNLARNPSNR
                     MQMTVFPDGEIGKEAITNYDTLETYYYTSLVNCRLETGRTHQIRAHMTYLGHPLFSDA
                     RYGGDEIKKGTIFSKYRQFVENCFKICPRQALHARTLGFVHPVTQENMYFECPLPDDF
                     NQLVEKWRNYIRSRKKDEIEP"
     gene            7666..8871
                     /locus_tag="PKV31_02970"
     CDS             7666..8871
                     /locus_tag="PKV31_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB91845.1"
                     /translation="MSEKNINNSLSISTILCSDCHQLPSEKPHILPLYSTSAYELGTL
                     ENSVGLFDGSKEGYVYSRYGNPTITALEEKLALIECGDSDGVAIMTSSGMSAISTILF
                     SLLSKEDVLVTHNGLYGGSTEILEILAIRIGFSLIYADLNDLESLESIKTQKAITAIY
                     FESPSNPLLHCINIIQLAEFSSKHGIRTICDNTICTPLLQLPLQKGIDFVIYSTTKYL
                     AGHGNSIAGCIISKHKDEMNANVRKNMRLLGGNCSPWEAWLTYTGLKTLELRMMRQCD
                     NAVQIASYLNNHPAVKAVYHNSIPDHPHHELAGRQMKHYAPLMSFELKGGLEAVSQFM
                     RRIRIITHAPTLGDLDTLVLHPDTSSHRNIPVEVKSKMGITPGLIRLSTGIEALDDLL
                     ADLDESLNV"
     gene            complement(8939..10111)
                     /locus_tag="PKV31_02975"
     CDS             complement(8939..10111)
                     /locus_tag="PKV31_02975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB91846.1"
                     /translation="MKINILASRFPYPLEKGDKLRLYHQIRLLSQHHELYLFCITDHK
                     TEEKDINHMRQFCKEVKLYPITMLKRIWLVFTGIFRGLPFHVGYFYQHSLKPMIHRDL
                     SATGADLTYCQLVRMVPYIKGFNSVIWLDFMDSFSRNLYNRLEKSGWWQKIWLRWELR
                     KMIEIEKASLGLTEHLSIISEQDRASLDPERKYNIVVLPNGVDTEYFHTNEYKDEIYD
                     LCFTGNLGYEPNIRAANYLIRRIGKRLKEDITIRIAGARPGRQLRSLNSSRFVVEGWV
                     EDIRDVYWTGKLFVAPLFTGSGQQNKILEAMACGLPVITTSQVNNAIGAEVGTEIFIA
                     DNLRTFVAQIDDLLDDEDLRHEVAYQARRFVCDNYTWEIIGEKLEDFLRGTERIGG"
     gene            complement(10134..10766)
                     /gene="pdxH"
                     /locus_tag="PKV31_02980"
     CDS             complement(10134..10766)
                     /gene="pdxH"
                     /locus_tag="PKV31_02980"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNB91847.1"
                     /translation="MDLHSYRQQYKKAGLDRHDLPEDPVQFFQALFIKAKEGGVIEPN
                     AMTLATGNADGFIDARMVLLKEVKNGDFVFFTNYNSVKGHQLASNPFAALVFWWYAIE
                     CQVRVRGTVTKLDESESDAYFNSRPHDSRAGAIASNQSEVVADYDKMMDNFEDLRNKS
                     DAELLRPSNWGGFCLQPAEIEFWQGRPNRMHDRFLYLREGEQWRINRLSP"
     gene            11054..11440
                     /locus_tag="PKV31_02985"
     CDS             11054..11440
                     /locus_tag="PKV31_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNB91848.1"
                     /translation="MNLTFGSKMDNITIGRLGEDIATAFLESKGYEILERNWRWLRIE
                     VDIIVMKDNTLTVVEVKTRTSNQYGDPDYFVDDRKLAILLDAAAHYAEKISHTWSIGI
                     DIVGILLHKDGSHSLKHIEDVYFPTF"
     gene            complement(11847..>12745)
                     /locus_tag="PKV31_02990"
     CDS             complement(11847..>12745)
                     /locus_tag="PKV31_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNB91849.1"
                     /translation="IRMESDREELPAFSDDVAPVVSDNRPDRKSKSETQNIAEVFYKE
                     EIREGLYLIFQYKMGQMDADSVLPVFLYNHSNQAIEFDMNYFSNNMPAQQYKGRLDPG
                     VIDLILHLPVSSLEHQPTFEIDWNLKADPGIKKQGDIVIRPAVFFKKYGHIAIINTSG
                     SMYYVQPVKAEKKAESLAGYTRAERKLHIPKPRREQSRTVREKAEFDPVLDLHIEKLY
                     PNPKSLKKGEILPLQLKCFDKFLENAIRLKIQQVFIIHGMGNGKLKDEIATRLVLHPG
                     VNTFKNEYHPSFGFGATEVWLY"
BASE COUNT         3906 a         2692 c         2434 g         3713 t
ORIGIN      
        1 cgttgccaac gaaacaaaat tattctcact tcttgccaat ataggcgaca caaaatccct
       61 tattatccat ccggcttcaa caacacatca gcagttgtcg gaagaagatc agggagcatc
      121 cggtgtaact cctgatcttt tacgaatatc tgttggactg gaaaacatag aggacctgaa
      181 agcagattta ttgcaagcat ttcaagccat ttattccaat agtcatgaaa aagttgaaga
      241 atatattaat caggaacttt cccttacctg atggtagcaa agaaagtttt aaagtttcct
      301 atcaaatttt tgggcaagaa ctccatactg ctccaattat cctaatcaat catgcactca
      361 ccggcaacag ttcagttgcc ggtgaggacg gttggtggaa agaggtggtt ggagactcca
      421 aggcgatcga tctaaggcga tatactgtaa tagcatttga tattcccggc aatgggtaca
      481 accatgttgc gcctatcgaa cgatacacat tactgacaac caagattgtc gggaacctgt
      541 tctggaaagc gttggacagt cttggtgtag tacaacttta cgccatcctg ggagggagcc
      601 taggcgggag tgttgcatgg gagatgttat ttcaaaggcc tgattccatc caaaagctga
      661 taccgatcgc atgtacattg aaaagttccg attggcttat tggcaatgca catgtacagg
      721 aaagcatttt gagcaattca caaaatccac ttgaagacgc aagaaaacat gcgatgcttt
      781 tgtacagaac tcctaattca ttcagggcaa aattcaaagg caggcaaagt gaatccggat
      841 ttgctgtaga gcattggctc atacaccatg ctgaacaact aaaatcaaga tatacgctcc
      901 catcctaccg tctgatgaac catttattga aaacaatagg agaaaaccgt gcaaaggatg
      961 aagttgagaa gatttttagg aattgtaaaa cggaaatcca tacaatcgca gttgattctg
     1021 attatatgtt tacccgtgaa gaacaaatag aaacgtataa aattattcgt aaatacaaga
     1081 aaaacgccag ttatggagag ataaagtccc tccatggcca tgacgcattc ttgatagaat
     1141 ttcaacaatt gaacaagctt ttaatatcaa tactctcatg aaagtaatta atctgaacca
     1201 atcttcttcc atacaacaag aacacagaca agtaactcag atcaaaaatt ttggaacaac
     1261 atcatcaata aacatatctt cggatgcgag aaagcctttg aggttcgact cagcaaggca
     1321 ggcagacaac aggcattgtc aggtaattaa aacgataaat attgccatct tcggcaccgg
     1381 caaggtaggg ggtactttaa tcgatcaaat actggcatcc ggcataaata ttgagaagag
     1441 taaaagtatt aggttaaaca tttttgctgt ggggaattct caaaaattac atctggacaa
     1501 atcaggtatc agtgaaaatt ggaaatttta tctcaataag tatggaaagc actacgacat
     1561 ctgggatata atcacgtatg ctcaggtcca tcaactggat aatctgatcg cagtagataa
     1621 tactgcctct gaagaattca ctgaaaatta tcatacttta gtcagaaatg gtttcgatct
     1681 tgtctcttcc aacaagattg gcaattcagg atcctatgaa ttttatcgca gtttaaggca
     1741 gattctccgc aagaatgaga gggaattcct gtacgaagcc aatgtaggag ccgggctgcc
     1801 attgattgac accatcagaa tgcttcatat ctccggagac aaaatccacc ggatacgtgg
     1861 tgtgttctcg ggtagcctca gttatatatt caatacgttt tcatcaaggg atatcccatt
     1921 tcatcaggtt ttacatgaag ctgcttcaaa aggcctcact gaacctgatc cccgggaaga
     1981 cctgtcaggc aatgatgtct gccgaaaatt gctcatcctg gcaagggaat tgggcctgag
     2041 ccaggaattc gagtccatcg acaatcacaa tcttattcct gaagaattag gcaaattgga
     2101 tctggatagt ttccaaaaaa acagccatga gctggacagg cactttgaat acctcaagca
     2161 gaatgtcaag ccggggcatg tcctccgcca tataggagag ttatccatca accgccaaag
     2221 ttgcgaagta ctgctcaaag cgggcttatc tgaaattccg gagtattcgg cattggggca
     2281 actaaccggt tctgacagct tgtttgaaat ttacacagaa ggctatggat tgaatcccat
     2341 aatcatcctg ggtgcaggag ccggacctgc agttactgcg cgtggtgtat tcagtgacat
     2401 attacgtttg gcaggcaggg tcatgaagaa ctaggtatta tgaagtattg gaatggagaa
     2461 tggcaatgtg ggcattaaat gaagggaaaa tggagatatg gttggggaaa atcgaagtat
     2521 gtacatgatt aataattgaa aactggcgag ctacaagggg tctggtaaat ctatcccaaa
     2581 aaaaataacc atttggacca aatttttcag aggtataaac taagaatacc atttcttggt
     2641 ggaatgctgg ctattaatca tctacctact tcaggtgaca gctcattcaa gatacaaatt
     2701 taattacaat gggccggaaa aatcgaagta tgtacatgat taataattgc aaactggcga
     2761 actacaaggg ggctggtaaa tctatccaaa aaaaataacc atttggacca aatttttcag
     2821 aggtataaac taagaatacc cttacttggt ggaaagctgg ctattaatca tctacctact
     2881 tcaggtggca gcatatttaa cactcacttc caagtctgac tccaacccag taatgcaaaa
     2941 gataaataaa aatgtcttgc gacgtccaat gtagtctgct caggttttac gccaattcaa
     3001 tgagtacttt ttgtttcttt ccattctcga caaagacata tccattgcca aatatttgtt
     3061 ccggttctga aattgcagct ggatcggtga tccttacccg gttgatctga acagcttgcc
     3121 cctggattgc tctttttgct tcaccttttg aagtgaaaat attcagttcc gtgagtagat
     3181 ctatgactga tgccccttgg ctccattgat cagcagaccc gctcctaacc tccaactccc
     3241 cttttaatag ctcgatagtg ttagtgccca ttgatttgac agcctgctca tcaaatccct
     3301 taccgaagag taaagaagaa acttttttaa cagcgtcaag gtcttcagca ctgtgtactc
     3361 gggttgtaat ttcttcggca aggatgcgtt ttacctcatt cggattgtca actaacatca
     3421 aatccaattc ttctatctcc tttttattct ttaatgaaaa ataacgcagc aacttaggca
     3481 tgtcttcatc actgcaatta atccaaaact ggtaaaattg gtatgcatta gtcatcccag
     3541 ggtctagcca tatattgcca ctttccgact ttccaaattt tgtaccgtcc gacttggtca
     3601 ataacggagt ggtagcggca tatgcctttc ctccaacatt tcttcttata aattcggtac
     3661 ctgctgtaat attaccccat tgatccgagc cccccatctg cacacgacag ttatatctgt
     3721 catatagtac ttgataatca tatccctgaa gtaactgata actaaattct gtgtaagaaa
     3781 taccggtctc catacgtttc ttaactgatt ctttacttgc catataattg acggtaagtg
     3841 ttttccctac atcacgaaga aacgtcagta cgttcatgtc tttgtagaaa tcgtaattat
     3901 tgacgaatac agctgcattt ggtccatcaa agtccagtaa ttttctgaat tgcttcattt
     3961 gacgatcgat attgacctgt atctcctcaa ttgtcttgag cgttcgttct gcatccttaa
     4021 aggaagggtc tccaatcata ccggtcgctc cacccatcag aacaatgggt ttattaccgg
     4081 ttcgttggaa atgagacaac aacataacct gaaccatatt cccaattgtc agggatggtg
     4141 cagttggatc aaacccaata taacctacaa tcggaccatc atcaaagatt tcttctatcc
     4201 ccggtgtata gtccatcaac atattccgcc acttcagctc ttcgataaaa ttcattgtat
     4261 aatattttga attacagcta cttgaataat attttcataa taaagtacct gaaaattttc
     4321 tgcaaaggta tcaaattgta gctttgctta tcaaatattt catgaattgc atttaagttt
     4381 cttcattgca gaccggatca acaggcatga aaacagtgcc ttcgcctcct ttatcatgaa
     4441 gctggcaatt gtttccgtag cacttagtgt atctgtcatg atcattacta cctgcctggt
     4501 gatgggcttc aagaatcaaa tcaagtccaa ggtatttgac ttctggggac atatccacct
     4561 caatttaccg gaatacaatc aggcgataga ccctaagcca cttaagctta acaattcatt
     4621 acttgactca atccgcgata tcaagagtgt accctttata aaggagaaaa gttttttagg
     4681 cattcctttt gggaatgtgg aaaaagagtc aaagacaggc attcagcatg tccagggatt
     4741 tgtacagctt cccgggatcc tgacttctga caatcaaatt gagggccttg tgctaaaggg
     4801 cgttaaccgt gattttgaca cttcctttct tgctaaatac cttgtcagcg gcaatggcga
     4861 tatcaaggat gattcgaccg gcaggacact catcattagc cagacaactg cttcaagact
     4921 caaattaaat ccgggagagg atattgtggt gtattttgtc gttggtggtc agcaaatcgg
     4981 acgaaaattc aagatcagcg gagtttacaa aaccggattg gaggagtatg acaggaaatt
     5041 cgctatatgt agctcaacag taattcaaaa gctgcttaac tggggacctg acgaatacac
     5101 caattatgaa atcttcgtcc agaatttgaa tgatctggaa gcaataaaca actatctgta
     5161 ctcagacgtg ttgcccacca acgtgtttag tacgacaatc cataacaagt ttcccggtat
     5221 cttcgaatgg cttcagttac aggattacaa tgaagtggtg attcttggtc ttatgattgc
     5281 cgtctgtctt atcaatatgg ctactgtatt gattatattc atactcaatc gattaaaaat
     5341 gatcggcata cttaaaacgc tcggcatgaa aaatcgggat ttgagaatga ttttcctact
     5401 tcaggcatcg cgaatcattc tatacggctt gatttggggc aatcttatcg gtttgggtct
     5461 ttcatggata cagtatcact ataaacccat caaacttaat gaagcagatt attatttgtc
     5521 cattgcacct atcgagtttt cctttccggc tataatgacc atcaatatcg taacattgac
     5581 catcattatg cttttcctga tccttccttc agctattgtt gcaaggattt cacctgtaag
     5641 gacaatcgaa tataattgat acatttgaac aatactatca ttgatatatt tttaggaaaa
     5701 gaatttataa ttattgatcc tgcattttac taaattattg gaagtaatat tttttaaaca
     5761 atagatttat ctggatttta tattagatat tattgatgat tttgttttca ccaaataaaa
     5821 ataaatatta agtgaccata tcgataattg tccgaaaagc gggattctat tttactaccc
     5881 tgcatattag aatactcaga ctcttcagac atattttctt tctgataaaa ttaacattca
     5941 gacccggtga gatcagtgtt cattattcgt ataggaaccg ttggctgact tggttgctgg
     6001 agttgccatt ctacgtaatt gatatattgg ccatacctga attgatggaa attttcttac
     6061 aattccttca tcctaaaaac cgtcttctta ctgaaagaga aaaaaaactg atcaatgagg
     6121 tgtatggtgg gtgccttaag ctatcggtca taagttttga tcgctatgca tctattatga
     6181 gggaccgcgc catggcattt gtaggggtta acacgatttt cttcaaccgg gaactctccg
     6241 atgccctttt attgcatgaa gtctgccatt gcctgcaata tcaaaaattc ggatccatat
     6301 atattatcag agcattgatg gcacaaaatt ctaaagaagg atatagttat ggagggagtg
     6361 attttctaca tgagtggaat ataacaagtg acctcaggaa gctgaattac gaacaaatgg
     6421 cggaaattat tggccatttc caccttatct ctctccgcca aatcaacgac gaagacgatg
     6481 taaggttacg taaattggaa atctatcgaa acgctgcagg caatattatc aacgcaataa
     6541 attatcaggg ttcaatttcg tccttttttc ggctgcgaat ataattacgc catttttcta
     6601 ccaattgatt aaagtcatcc ggcaatggac attcaaagta catattctct tgagtcactg
     6661 gatgtacaaa acccagagtt cgtgcatgaa gagcctgacg gggacatatc ttgaaacaat
     6721 tctctacaaa ttggcgatat tttgaaaaaa tagtaccctt ctttatttcg tctccaccat
     6781 aacgggcatc agaaaacaat ggatgaccca aataagtcat gtgtgccctg atctgatgtg
     6841 ttctcccggt ttccaaccgg caattgacaa ggcttgtgta ataataggtt tcaagggtat
     6901 cataattagt aatagcctcc ttcccgattt caccatcagg aaaaaccgtc atctgcatcc
     6961 tgttagacgg attacgggca agatttccta ctattcggcc ggcaagggga tccaattccc
     7021 cccaaaccag tgcagtatat tctcttcgga ccgtatgatc aaaaaattgc ttggccagat
     7081 gggacattgc atattctgtt ttggctatca ccattaagcc cgttgtatct ttatcgattc
     7141 tatgcacaag gccgggtcgg tctgcaatat tcccttcttt gataggcaga tcaaacttaa
     7201 agtaatgagc caaagcattg actaaggttc cggtcttgtt gcccaaacca gggtggacta
     7261 ccattccggc cggtttatta atgaccataa catcgtcatc ttcataaaca atattaagcg
     7321 ggatatcctc tggttcaacg gcatattcac caggaggttc aggcaggaca ataacgatgt
     7381 tgtcgttggg ccttacctta taattaggct tgacaggctt atcgttaacc aaaacgcctc
     7441 ccgaacggat aacattctgg accttattgc gggtgatatt cataatccga tcaagcaaga
     7501 acaaatcaat ccgaatggga gtctgtccct tatccactgt cagtgcaaaa tgaacaaaaa
     7561 gctcatctcc ttgtcctgat tcattctcat caatctccct caactgttcc ataacaattc
     7621 aaaatagctg aaaactatta cctttaacat caaataaagt tgcaaatgtc cgaaaaaaat
     7681 atcaacaatt cattatcgat cagtacaata ctatgtagtg attgccatca attaccaagt
     7741 gaaaagccac acatattgcc tctgtatagt acttcagctt atgaattagg aactttggaa
     7801 aattcagtcg gtctttttga cggttcaaag gaaggctatg tttattccag atacggaaac
     7861 ccaacaataa cggcattgga agaaaaactc gcactaatcg agtgtggtga tagtgatgga
     7921 gttgcaatta tgacctcatc aggcatgtca gctatttcga ccatcttgtt ttccttactc
     7981 agtaaggaag atgtacttgt aacacataac ggactgtatg gcggcagtac agaaattctc
     8041 gaaattcttg ccattcggat tgggttcagt ctgatatatg cagacctgaa cgacctggaa
     8101 tcactggagt caattaagac acaaaaagca ataacggcta tttatttcga gtcgccctcc
     8161 aacccattac tacattgtat aaatatcatc caactggcag agttttcaag taagcacgga
     8221 atacgaacta tttgtgacaa taccatatgt acacccttat tacagttgcc gctacaaaaa
     8281 ggcatcgatt ttgtaattta tagtacaacg aaatatcttg ccggtcatgg aaacagcatc
     8341 gcaggttgca tcatcagcaa acataaggat gaaatgaatg caaatgtcag aaaaaatatg
     8401 cggttgctag gaggcaattg ttcaccgtgg gaagcctggt tgacttatac gggcttgaaa
     8461 accctggagt tgcgaatgat gcgacagtgc gacaacgctg tacaaattgc ctcttaccta
     8521 aacaaccacc cggctgtaaa ggcagtgtat cacaattcaa ttccagacca tccacatcac
     8581 gagcttgccg gaagacaaat gaaacactat gctcccttga tgagtttcga actcaaaggc
     8641 ggattagaag ctgtctccca atttatgcga agaatacgca tcatcacaca cgcgccgaca
     8701 cttggagatc tcgatacctt ggtgttacat cctgatacat cctcacaccg aaatatacct
     8761 gtcgaagtaa agtctaaaat gggtatcact cccgggctga ttagattatc aacaggtata
     8821 gaggctttgg atgatttatt ggctgatttg gatgaatctt tgaatgtata aattggtcta
     8881 ctgggcattt ttattaatta ctacaaaatc atcaaatctc gagatatctt caatcttctc
     8941 accctccaat gcgttctgtg ccgcgaagaa agtcctccag tttttcaccg ataatctccc
     9001 aggtataatt atcacataca aatctccttg cctgataggc tacttcatgt ctcaagtcct
     9061 catcatccag caagtcgtcg atttgagcaa caaacgtccg aaggttatct gcaatgaata
     9121 tttccgtacc cacctcggca ccaatggcat tatttacctg agatgttgtg atgacaggca
     9181 gtccacaggc catagcctca agtatcttat tttgttgccc acttcctgta aacaagggtg
     9241 caacaaacaa tttaccggtc caatacacat ctcgaatatc ctccacccat ccttccacaa
     9301 caaaacgcga gctattcagg ctgcgtaact gcctgcccgg ccttgcaccg gcaatgcgta
     9361 tggtaatatc ctccttcaac ctttttccga tacgccgaat cagataatta gccgctctga
     9421 tgttgggctc atagcctaga ttgccggtaa agcacaagtc atagatctca tccttatact
     9481 cattggtatg aaagtattca gtatcgaccc catttggcag tacaacgata ttgtattttc
     9541 tctccggatc gagggatgct ctgtcttgtt cagaaatgat acttaggtgt tcagtcaaac
     9601 ctaaggaagc tttctcaatc tcaatcattt ttctcaattc ccaacggagc catatctttt
     9661 gccaccatcc tgacttctcc agcctgttat acagattccg agagaaagaa tccataaaat
     9721 ccaaccagat cacactatta aaccctttga tatagggtac catacggacc aattggcaat
     9781 aggtcaaatc ggcaccggta gcggacaagt ctcggtgtat cataggtttg agactatgtt
     9841 ggtagaaata tccgacatga aatggaagac cgcgaaatat tccggtaaaa accaaccata
     9901 tccttttcaa cattgtaatg ggatatagct tcacttcctt gcaaaattgc ctcatgtgat
     9961 taatgtcctt ttcctccgtc ttgtgatctg tgatacagaa caaataaagt tcatgatgct
    10021 gtgacaacag acggatctga tggtagagac ggagcttgtc gcctttttcc aatggatacg
    10081 ggaagcggga agcgagaata tttatcttca aagtatcatc ttttggtaaa aaatcaagga
    10141 ctaagtcgat tgatacgcca ttgttcgcct tctctaaggt ataaaaagcg atcgtgcata
    10201 cgattaggtc ttccctgcca aaattcaatc tctgcgggtt gcaggcagaa gccgccccag
    10261 ttagaaggac gaagaagttc tgcatcgctt ttgttacgca ggtcttcaaa gttatccatc
    10321 atcttatcat aatcggccac gacctcactc tgattggaag caattgcacc tgccctgctg
    10381 tcatgcggcc tgctgttaaa atatgcgtct gattcagatt catcaagctt ggtgactgtg
    10441 ccgcgaactc gtacctgaca ctctatggca taccaccaaa aaactaatgc agcaaaagga
    10501 tttgatgcta attgatgccc tttgacagaa ttatagttag taaaaaagac gaaatcacca
    10561 ttcttaacct cttttaacaa aaccatcctg gcatcaataa aaccgtcggc attgcccgta
    10621 gcaagagtca ttgcattggg ttcaattaca ccaccctctt tggccttgat gaaaagggct
    10681 tgaaaaaatt gaaccggatc ttcaggcaaa tcgtgcctgt ctaatcctgc tttcttatac
    10741 tgttgccgat aactatggag atccattatg cttttgggat aaaagccaaa gataaaacag
    10801 aaatcacaat ttgcgaataa agtttgaagt aaaatcggca acttaaaaca ttcccaggta
    10861 cattacaaaa caatcgtagc taaaacagaa aaaatccggg cacctaaccg atgtccggat
    10921 taaaccatga aattaaaata aaacgattta agacaattac cttataattc tgttgaacaa
    10981 gtcttccaaa ttacaaacaa caaaatatag acgtatcaat attaggctat attgcctgca
    11041 aaatgttaat tttatgaatt taacatttgg ttccaaaatg gataatatta ccatcggacg
    11101 attaggagaa gatatcgcaa ctgctttcct tgaaagcaag ggatatgaaa ttctggaacg
    11161 caactggagg tggctccgga ttgaagtcga catcatagta atgaaggaca acacacttac
    11221 tgtcgtagaa gtaaagactc gaacttccaa tcaatatggc gatcctgatt attttgttga
    11281 cgaccgaaag ttggcaatat tgctggatgc tgctgcacat tatgcagaaa aaatcagcca
    11341 cacatggtct attgggattg atattgttgg catactgtta cacaaggatg gctcccatag
    11401 cttaaaacac atagaggatg tatatttccc tacattctga ttgattcaac aattcattta
    11461 aatctaatga gataaccctt cctaaagtag cctggaatgc agcacacaca tcatttgata
    11521 tgattaggag gagaggtaaa gcggaacctt aaaggacatg gtcagcaaag ggaaattcga
    11581 agtatgtaca tgattaataa cctcccattt atgaattacc gaccattcaa gatgctaaat
    11641 tttattcaaa ctctcgtatt cggcaataaa tggatattac ttcctcaaat tatcctacat
    11701 aacaataatt agggttatta ttttcctacc tacttcatat tgacaaaaac gctctttttt
    11761 ttcatgcctt ggcaattcta tctcctcaac attctgtgct ctatattatt gataaaacgt
    11821 tttatttctc ttcgtaatct tacagatcag tataaccata cctcggtcgc tccaaagcca
    11881 aaacttggat gatattcatt tttgaacgtg ttgacgccgg gatgcagtac gagtctggtc
    11941 gctatttcat ccttcaactt tccatttccc attccatgaa tgatgaatac ctgttgaatc
    12001 ttcaatcgta tagcattctc caaaaactta tcgaaacatt tcagttgcaa cgggagtatt
    12061 tcacctttct ttaatgactt tggattagga tacagttttt cgatatgtaa gtcaagtacg
    12121 ggatcaaatt ctgctttctc acgtactgtg cgcgattgtt cacgtcgggg tttaggaatg
    12181 tgcaatttcc tttcagctct ggtatagcca gccaatgact ctgccttttt ttcagcctta
    12241 actggttgta cataatacat acttccgctg gtgttgatga tagctatatg gccatatttt
    12301 ttgaaaaaaa cagcaggcct gatgactatg tcaccttgtt tcttaatacc cggatcagcc
    12361 ttcaaattcc aatcaatttc aaaggtaggc tggtgttcca atgaagaaac tggtagatgt
    12421 aaaatcaggt cgataacacc cggatccagc cggcctttat attgctgagc tggcatatta
    12481 ttcgaaaaat aattcatatc aaactcaatt gcttgattgg aatgattgta tagaaataca
    12541 ggcagcacag aatctgcgtc catttgaccc attttatact ggaaaataag ataaaggcct
    12601 tcacgaattt cctctttata gaagacttct gcaatgtttt gagtttccga cttactcttt
    12661 ctatccggcc tgttatcgga aacgacagga gcgacatcat cagaaaaagc aggcaattcc
    12721 tcacgatcac tttccatacg gatat
//
LOCUS       DAOQMC010000042        12543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_1916_length_12543_cov_4.957479, whole genome shotgun sequence.
ACCESSION   DAOQMC010000042 DAOQMC010000000
VERSION     DAOQMC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12543
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1916_length_12543_cov_4.957479"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKV31_02995"
     CDS             <1..561
                     /locus_tag="PKV31_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB91850.1"
                     /translation="IRSSDTHTIMYNDVKVPKENRIGEDGFGFKFAMKVLSGGRIGIA
                     SQALGIASGAFELATAYSKERKSFGKEIYNHQVIAFKLADMATEIEAARLLVHRAAWL
                     KDQKKDYNTASAMAKLFAAETAMKTTVEAVQIHGGYGFVKEYHVERLMRDAKITQIYE
                     GTSEIQKLVIARSVINGELYLPGYVV"
     gene            584..1696
                     /locus_tag="PKV31_03000"
     CDS             584..1696
                     /locus_tag="PKV31_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNB91851.1"
                     /translation="MAIFEKGSMYAGTYFGIPVKIHWTFGYIIFYIIYSGYQDNLGLQ
                     GMLWFGLFILSLFLCVILHEYGHALTARRYGVHTADIILTPLGGIARLTKMPDKPVQE
                     LIVALAGPLVNVIIAGLLFVGLYFGFEYNPFIIDVDNAYNFTGNPGGFFGMLMLTNIA
                     LVVFNLLPVFPMDGGRVLRSLIAMKASKVRATLIAARTGQLFSLFFVGYGIYSGNLML
                     ALIGLFIFQSAYMEYRYTKADNLMQSSIVENLGYAEYDTFQSDDLIINCKELMKYSDQ
                     PFFPILSNEVVIGSVSRDDIIQSDLLYRPVSELSMMPVPQLPADTNWMTCYQIFSKDQ
                     ADVILLNKDGAKPTLLDKWIFYQQLENKKLIKKGKL"
     gene            complement(1718..2503)
                     /locus_tag="PKV31_03005"
     CDS             complement(1718..2503)
                     /locus_tag="PKV31_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB91852.1"
                     /translation="MIFFLITFSGQAQDAIPPYDSLVIQSELLGESRAINIWTPPGYA
                     ISKASYPVLYMPDGAITEDFPHIANTLSELILAGKIPPTILVGIPNTQRRRDLTGPTT
                     VRRDKKIAPVVGGSTAFRMFIRDELFPEIASRYRTNGQRTIIGESLAGLFIIETFLMA
                     PDMFDNYIAFDPSLWWNKHQLVNGAAESLKHFPKAEKRLWFAGSSVQDIYPHTRQLAE
                     ILRNANLGNLKVNYSDEPGEKHNTIFRATKVKALEWTLGNDSR"
     gene            complement(2880..3617)
                     /locus_tag="PKV31_03010"
     CDS             complement(2880..3617)
                     /locus_tag="PKV31_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91853.1"
                     /translation="MTEQKQFVATEEAKGQAKQLRLFAMLAWIVAIAGEVFAILKLLR
                     NDMLTWLIVTIVGILALSIVGSILWKRANRLDPASEKDKTRFFIQNQLGAIMGVLSFL
                     PLVILIFTNKDLDKKTKGIAGAVGVVALLIAGITGVDFNPPSIEKYTEQMNAQTATLK
                     DLNNGVDMVYWTNQGNKYHIFEDCQHIKNREIHNGTVEDAFKARKIGDNELCKTCMAR
                     AEKMKTGTPAGNEETVPAPVPEEATQN"
     gene            3857..4834
                     /locus_tag="PKV31_03015"
     CDS             3857..4834
                     /locus_tag="PKV31_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91854.1"
                     /translation="MKRAIHIILFLFGLTSLVPLQAQTKVELNISHLFGHQPFAPENV
                     GINDLNTPFKIERLEYYISDIILVHDGGVETKVPDYWILADISKRGSYELGSFDIKHL
                     EKVIFHIGVDPEHNNADPSVYPADHPLALKEPAMHWGWAAGYIFIALNGNGGDNLDNP
                     FQVHALGNENYYAVELEMNIDAVNGSLTIPIDANYDLAMKGMDASIGIFYHGPTKFGA
                     ILATNFANYVFSKRKSPASTHSEAKTGTQFLLYPNQLTGGSGLLLIEGKFDHAADLII
                     TNGLGKVVRKYTKVHSGATIEINDLKPGIYFASLVKGGMPSGTQKLLIF"
     gene            4907..5941
                     /locus_tag="PKV31_03020"
     CDS             4907..5941
                     /locus_tag="PKV31_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNB91855.1"
                     /translation="MSAIPFRITSIVMLLGALLSCQKEEAENEAFPALMQVPTYFPEV
                     PQPADNHFTVDRWNLGRKLFYDPLFSKDSTVSCGSCHRQQAAFGDDKALSEGINGLVT
                     TINVPALINIAYQPYYTGSGGVPTIEMQVLVPIQEHNEFNFNMPGIVKRIKSIPEYVS
                     LSMKAYGREPDAFVVTRALGCFERSLISGQSRYDKYRETGDLSKLSSEAIRGMALFNS
                     EKCGCSKCHSGINFTNYAFENNGMYTFNPEDGRYRVTLNDQDMGKFKVPTLRNIEYSA
                     PYMHDGTLTTLEDVIRKYEAGGNDYATKSPLIKGFQLSSKERNDLISFLKSLSDRNFI
                     TNPEFSPEDF"
     gene            6275..8902
                     /locus_tag="PKV31_03025"
     CDS             6275..8902
                     /locus_tag="PKV31_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB91856.1"
                     /translation="MLTVKRFRKSLFVLFLFCSASLCHHGFSQNLNLSLIKPINQKVL
                     NDYGEGVYFENFEPFRHSVASRTRSIDRKDLSDFYYVEIKSDELGKLNKNRSASVRMT
                     IPFGDREISMLLTRSEVLSEDFRLFTSGTGEIPQDYKPGLYYRGVVEGENNSLVAISV
                     FEDNIIGMVSTEASGNIVISQTENRGVYLLYSDRDLKMQNPTSCAAVEPDHYAEEIAQ
                     LLNNQLQTRDEKCFKLYLECDYALYQNKGSVSAVTDWVTALYNNVAALYQNESLLTKI
                     SEIYVWTTPDPYSKTSSYTALTQFKTQRPNFNGDLAQLVALGGNNIGGIAWIGGLCTA
                     YKYSYANIQNYYNSVPVYSWTVEVMTHEIGHNLGSPHTHSCSWPGGALDNCYPVEGNC
                     NPGPPPTTGGTIMSYCHLTNYGINFNNGFGQKPGDLIRAKVNASSCLGYCDDGGGPMC
                     GAPQSVTISNITNNSAMVSWTTGLNGIYYIVEYKVSTSNTWTILPQQSSTSLLLSGLN
                     SGTTYHVRIKTICSEGESEYSAVYTFTTGNACGIPYGPFVQNITETSAYVYWKPVPGA
                     TSYDFRFKLTSSNNWATINTSSTSISLSGLTPGTSYDVGVRARCGTVYSDYTGTYTFV
                     TLGNPPGGGSGYCDSHGTSSAFDWIQKLTFGSFTNESGNNNGYGDFLNQTISAKKNTM
                     VIISVTPGMIGNYTEFWTVWIDFNQNESFTDPGEQVATFTTTNTNPNYTKIFIPDNAS
                     LGNTRMRIQMKRGSFSTSCEAFPYGEVEDYTLSITQQLKGGTSEYSSSNNTTLFPNPF
                     NENFTLNFESENAEDARIEISDITSRIIFSHPMSVQKGLNSFRLEDTSSMEPGIYMVR
                     IIGQNINETHKAIKVSNRN"
     gene            complement(9250..9774)
                     /locus_tag="PKV31_03030"
     CDS             complement(9250..9774)
                     /locus_tag="PKV31_03030"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNB91857.1"
                     /translation="MIVSAIVAVAENGVIGDRGAMSWHLKSDFKNFKKKTLHHPIIMG
                     RTTFESIGRPLPKRENIIITRDMFYLASGALIAHSVEEAMDLAARTGNEEVFIIGGAE
                     IFRQTIGLWDKLYYTEVHMVARGDTFFPMINWDEWVLTERIYQPKEENDDSDCTFKVF
                     LRSGRFDSHGISDY"
     gene            complement(9771..11633)
                     /gene="mrdA"
                     /locus_tag="PKV31_03035"
     CDS             complement(9771..11633)
                     /gene="mrdA"
                     /locus_tag="PKV31_03035"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNB91858.1"
                     /translation="MVAKPDSRIRIIQMVFIMSAMVLVMKAAQIQLFDTSFQVRADAT
                     AIEKHVVYPARGIIYDRNGKLLVNNDPMYDLMVTYNAIDPNMDTAKFCNILGIDKETF
                     IANLSKDWASKRFSKSIPFPFLKKISPYTYTRFQESLWEFPGFFPQLRIARGYPHHNA
                     ANVLGYIREVDQNQIASSGGLYGLGDYIGISGMEGQYERLLMGRKGIQYIMKDNMGRP
                     VGPYRNGAQDSIPVSGQDLISSIDLDLQQYAESIMQGKRGSVVAIEPQTGEILAAVSS
                     PSYDPNLLTINQGRGKAFGELINDYNKPLYNRIAQAKYPPGSIFKTVVALSAMQMGVL
                     TPNRSISCHGAYYYRGGKVKCHAHPASVNVSHALQYSCNTYFITVLRDAVDKYGFTRP
                     SKGLTELDRHLFDFGLGHPLGTDMMHETKGNIPTPAYYEKIYGKDWRSTYMMFIGIGQ
                     GEIQLSALQIANLAAVIANRGYYYVPHLIKGFKNKEMEIPEQFREKHMSGVDAKFFKP
                     VIDGMDAAVASKWCGWSTPIPGITLCGKTGTSQNPPYRDHAVFMAFAPKDNPKIAIAV
                     LIENAGFGAQSAAPIASLIVEKYLRGYIEAPFRKEREKEMTRLNLLGPPKLDKK"
     gene            complement(11633..12154)
                     /locus_tag="PKV31_03040"
     CDS             complement(11633..12154)
                     /locus_tag="PKV31_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91859.1"
                     /translation="MGGTIARNLVRFVFILLLQVLVFRSLTIYYPVLDNISIIIYPVI
                     IMLLPMRTPGALILVIAFVLGILVDMFYNTPGVHASACVFTGFIRPFVLSRLEPRGGY
                     TVDSSPTIIAYGNRWFLFYSSILLLLHLFFLFSVQKFTFVYFLEIITRTFLSFIASMI
                     FILVYMYLFNPKE"
     gene            complement(12160..>12543)
                     /gene="mreC"
                     /locus_tag="PKV31_03045"
     CDS             complement(12160..>12543)
                     /gene="mreC"
                     /locus_tag="PKV31_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNB91860.1"
                     /translation="VRQTSRNYATVASLLGSQTKISAAIKRNGYFGSLVWDNTSTEYV
                     YLTDIPKHADILKGDSVITSGYSTTFPRGIYIGRVENVGFDPGSSFYDLKVKLTTDFN
                     NLTYVYVILDKTKPQLDSLNNAILR"
BASE COUNT         3773 a         2692 c         2572 g         3506 t
ORIGIN      
        1 atccgctcat cagacaccca caccattatg tacaacgatg tgaaggtgcc aaaggaaaat
       61 agaataggcg aagatggatt cggattcaag tttgcgatga aggtattgtc aggaggtcgc
      121 atcggcatcg cttcacaggc tttgggcatc gcatccggtg cttttgaact tgccactgcc
      181 tatagtaagg aaagaaaatc attcggcaag gaaatataca accatcaggt cattgcattt
      241 aagctggccg atatggccac ggaaatcgaa gcagccagat tgcttgttca cagagctgca
      301 tggttaaaag atcagaaaaa ggattacaac acagcctcag ccatggctaa gttgtttgct
      361 gcggaaacag cgatgaagac aaccgttgag gcagtacaaa tccacggtgg gtacggtttt
      421 gtcaaggaat atcatgtgga gcgtttgatg cgcgatgcca agattactca gatctacgaa
      481 gggacaagtg aaatacagaa acttgtcatc gcccgcagcg tcattaatgg tgaactatat
      541 cttccgggct acgttgtata gtacacaaat caaacgctga atattggcta tatttgaaaa
      601 aggttcgatg tatgccggca cctactttgg tataccggta aagattcatt ggactttcgg
      661 ctacattatc ttttacatca tctactcggg ctatcaggac aatttgggct tacagggaat
      721 gttatggttt ggcctgttta tcctgagtct ctttctgtgt gtcattcttc acgaatacgg
      781 ccacgcactt actgcccgga gatatggggt acacaccgcg gatatcatcc ttacgccctt
      841 gggtggcatt gcccgcttga caaaaatgcc tgacaagcct gtgcaggaac tcattgtcgc
      901 tctcgcaggc ccactggtca atgtaatcat agccggactg ctatttgtag gattgtactt
      961 tggctttgaa tacaacccat tcatcattga tgtagacaat gcctataact tcactggcaa
     1021 tccgggcggc tttttcggaa tgctaatgtt gaccaatata gcattggtgg ttttcaacct
     1081 gttgcctgta tttcctatgg atggaggtcg cgtgctgcgt agtctgattg ccatgaaggc
     1141 gagcaaagtc cgcgccaccc tgattgcagc cagaacagga cagctcttct ccttattttt
     1201 tgtaggttac ggaatctata gcggcaactt gatgcttgca ctgatcggac tctttatttt
     1261 tcagagtgcc tatatggaat atcgatatac caaagctgac aacctcatgc aatcctccat
     1321 agtcgaaaac ctcggttatg ctgaatacga tactttccag tcagatgatc tcatcatcaa
     1381 ttgtaaagaa ttaatgaaat attcagatca gccatttttc cccatccttt ccaatgaagt
     1441 tgtcataggg agtgtatccc gggatgacat catccaatcc gatttgttgt ataggcccgt
     1501 ctcagagctg tcgatgatgc ctgtacctca gcttcctgcc gataccaact ggatgacttg
     1561 ttaccagata ttctcaaaag atcaggccga tgtcattctt ttgaataagg atggcgcaaa
     1621 accaaccctt ttggacaaat ggatcttcta ccagcagttg gaaaacaaaa aactaatcaa
     1681 aaaaggtaag ttataataaa ttccggtatg gtaatactta tctggaatca tttcccaatg
     1741 tccattccag cgctttgact tttgtggctc tgaagatagt attgtgcttc tctcctggtt
     1801 catcggagta gttcaccttt aggttgccca gattggcatt cctgagtatt tccgccaatt
     1861 gcctggtgtg aggatagatg tcctgcaccg aagaacctgc aaaccataat cttttttcgg
     1921 ctttcggaaa atgcttcagt gattcggcag cgccattgac cagctggtgc ttattccacc
     1981 acagcgatgg atcaaacgca atataattgt caaacatatc cggtgccata agaaaagtct
     2041 ctatgataaa caggcctgcc aaagattctc cgatgatcgt tctttgccca ttggtcctgt
     2101 atcttgacgc tatttcggga aacagttcgt cacgaataaa catgcgaaat gccgtggaac
     2161 cacctacaac aggcgcaatc ttcttatccc ttctgacagt tgtcggacca gtcagatccc
     2221 gtctccgttg ggtattaggg atgcctacca gaatcgtagg gggtatcttt ccggcaagta
     2281 tgagttctga aagggtgttt gcaatatgag ggaagtcctc tgtgatggcg ccatccggca
     2341 tatataggac aggataagac gctttactga tggcatagcc gggaggagtc cagatattga
     2401 ttgcacggga ttcgcctaat aattccgatt ggatgacgag gctatcgtat gggggaatag
     2461 catcttgagc ctgtccggaa aaggtgatta aaaaaaatat cattgaaata aaaatactat
     2521 gccgcgatat tgtcatctat cattcatttt atgttaaaaa caaagtaaaa gtaggtaaaa
     2581 ttgaacatta aatttgttca aaaaattgca cttcttctgt tcaaaatatt ttggtttgct
     2641 ttggtcgatc cgtccgggac tttgagacac accatcaccg cccaaagaat ttgacaaaga
     2701 actgcccggt aaccagcatt tccggaaaca accaaactac ttagtattcc gaaaagctat
     2761 tcttgaaagg aaaagaagat agtcttagaa tataacaaca atccactaaa ttgcttaagc
     2821 taaatatcac aaaaaaaagc ccactttctt gtgggcttat tcttccgatt cccggattat
     2881 taattttgtg tggcttcttc cggaacagga gccggcactg tttcttcatt tccggcagga
     2941 gtcccggtct tcattttctc agccctggcc atgcatgttt tacacaattc attatctcct
     3001 atctttctgg ctttgaaggc atcttctact gtaccgttgt ggatctcgcg attcttgata
     3061 tgctgacaat cttcaaagat atggtattta ttcccctggt tggtccagta caccatatct
     3121 acaccattat taagatcttt caaagttgca gtttgagcgt tcatttgctc ggtatacttc
     3181 tcaattgaag gcggattaaa atccacaccg gtgatgcctg caatcaatag agctactacg
     3241 ccaaccgccc cggcaattcc cttggtcttt ttatccaggt ctttattagt aaagatcaga
     3301 ataacaagcg gtagaaaaga cagaactccc attatggcac ccaattgatt ctggataaaa
     3361 aatcttgtct tatctttctc agaagcggga tccagtctat tggccctttt ccaaagaatc
     3421 gaacctacaa tagatagagc caggatgcct acgatcgtca cgattagcca ggtgagcata
     3481 tcatttcgca aaagcttcaa aatggcgaat acctcacctg ctattgcaac gatccaggca
     3541 agcatcgcaa aaagtcgcaa ttgttttgcc tgaccttttg cttcttcagt tgctacgaat
     3601 tgtttctgtt ctgtcataat acttttattt gaattttact tatatgaaaa gtaaagatat
     3661 atacttaaaa gaatttatca aaattttgtt ttgaatcaag caggaattca gattttgtac
     3721 cgatgttccc tttgaaaatg tgtctccctg ataaagcacc tgtcaacaaa atgtaatgaa
     3781 gcaatggatc aagatgtatc tataaatcaa atgcataatt tttaataaat ttgtgacaat
     3841 taaatgataa tcaatcatga aaagagcaat acatatcata ctttttttgt ttggccttac
     3901 ctctctggta ccgcttcagg cccagaccaa ggtagaatta aacataagcc accttttcgg
     3961 ccaccaacca tttgcccctg aaaatgtcgg tatcaacgac cttaacacgc ccttcaaaat
     4021 agagcgattg gaatactata tttctgacat tattctggtc catgatggag gtgttgagac
     4081 aaaggtcccg gattattgga ttctggcaga tatatcaaaa agggggagtt acgagttggg
     4141 aagctttgac atcaaacacc tggaaaaagt catcttccat ataggcgtgg atcccgaaca
     4201 caacaatgcc gacccttccg tctatccggc agatcatcca ctggcactga aggagccggc
     4261 aatgcactgg ggatgggcag caggctatat cttcatagca ttgaatggca atgggggaga
     4321 caaccttgat aatcccttcc aggttcatgc gctgggaaat gaaaactatt atgctgttga
     4381 attggagatg aatattgacg ctgtcaatgg cagtcttaca atccccatag atgccaatta
     4441 tgatctcgcc atgaagggaa tggatgcatc cattggaata ttctatcatg gccctacaaa
     4501 attcggtgcc atattggcga ctaactttgc caattatgta ttcagcaaga ggaagtctcc
     4561 tgcatctacc cattcagagg caaagacagg aacacaattt ctgctgtacc ctaatcaact
     4621 gacgggtgga agcgggcttt tacttattga aggtaaattt gatcatgccg ccgatctgat
     4681 cattaccaat ggattgggta aggtggtcag aaaatataca aaagttcatt ccggtgccac
     4741 tattgagata aatgacctta agccgggcat atattttgca tccctggtga aaggcggtat
     4801 gccttcgggc actcaaaagc ttttgatatt ctaaagtaat gtcagtataa ccaagattgc
     4861 gatgagcctg gtaagttgaa gtaatgacta tagaggaaca tgaatcatgt cagcaattcc
     4921 attcagaatt acaagtattg ttatgctgct cggggctctt ctttcctgtc aaaaagaaga
     4981 ggctgagaat gaagcctttc cggcattgat gcaggttccg acatattttc ctgaagttcc
     5041 acaacctgca gacaatcatt ttacggtcga tcgatggaat cttggcagaa agttgttcta
     5101 cgacccactt ttttcaaaag acagtacagt cagttgtggt tcctgccatc ggcaacaagc
     5161 tgcttttggt gatgataagg cattgagtga aggaatcaat ggtctggtga ccacaatcaa
     5221 tgtacccgct ctgattaata tcgcctacca gccctattat accggatcag ggggagtgcc
     5281 taccatcgaa atgcaagtat tggtaccaat tcaggaacac aatgaattca acttcaatat
     5341 gcctggtatc gtaaaaagaa taaaaagtat tcctgaatat gtgtcccttt ccatgaaagc
     5401 atatggccgc gaaccggatg catttgttgt cacacgggca ttagggtgtt ttgaacgtag
     5461 cctgatcagc ggacaaagca gatatgacaa ataccgggaa acgggagatc tctcgaaact
     5521 gtcttctgag gctatcagag ggatggcact attcaacagt gagaagtgtg ggtgttcaaa
     5581 atgccattcc ggaataaact ttacaaatta tgcatttgaa aacaacggaa tgtacacttt
     5641 caatcctgaa gatggaagat atcgtgtgac gctcaatgat caggatatgg gaaaattcaa
     5701 ggttcccacg cttagaaaca tcgaatactc ggcaccatat atgcacgatg gcacccttac
     5761 aacgttagag gatgtcatcc ggaaatatga agccggagga aacgattatg caacaaaaag
     5821 cccgctcatc aagggatttc aactctcttc caaagaaaga aacgacttga ttagtttctt
     5881 aaagagtctt tcagatcgta atttcataac aaatccggaa tttagccctg aagactttta
     5941 atggaattta ttctttacaa acacatccag gcgacaacta cataatgcgc tgaatatcca
     6001 tgtgttataa atattttatt aatcagaatt tagaatttct taccaattgg gataaaaaaa
     6061 cctcaatttt actttgaaat cacaaataca aaatatagtt ttgttctgct taaataggca
     6121 atatattaac ttaagatcaa aacgattgat ttatatataa ttttatttta ataagttcac
     6181 acacttacaa tattattatt tctcaaaagc tcaaaatgtt gggtttttat aaatataata
     6241 atttatctct tattaacttt ttaaattgta agtcatgtta actgtaaaac gctttaggaa
     6301 aagcttattt gtactgtttc tgttctgttc ggcatcgtta tgccatcacg gattttcaca
     6361 aaatctgaat ctttcattaa tcaagccaat caaccaaaag gtattgaatg attatggtga
     6421 gggagtatat tttgaaaatt ttgaaccgtt tcggcattcg gttgcatcaa gaacccggag
     6481 tattgaccgg aaggatttat cggactttta ttatgttgag ataaagtctg acgaattagg
     6541 taaattaaac aaaaaccgca gtgcttcagt caggatgacc atcccatttg gggacaggga
     6601 aatatctatg ttgttgacca gatctgaggt attgtctgag gatttcaggc tgttcacatc
     6661 cggcaccgga gaaatccccc aggactataa gcccggatta tattatcggg gtgttgtcga
     6721 gggtgaaaat aattctctgg tggcaattag tgtctttgaa gataacatca tcggaatggt
     6781 atccacagaa gcaagtggaa atatcgtcat atcacaaacc gagaatcgag gtgtatattt
     6841 gttatattcc gaccgcgatc taaaaatgca gaatccgaca tcctgcgctg ctgttgagcc
     6901 tgatcattat gcggaagaaa tagcacaact gcttaataac caattgcaga ccagggatga
     6961 aaaatgtttt aaactatatc ttgaatgcga ctatgccctc taccaaaaca aaggatcagt
     7021 atcagctgta acagattggg taacagcctt atataacaat gtcgccgctt tataccagaa
     7081 tgaaagtctt ctgactaaaa tttctgaaat atatgtctgg acaacaccgg acccctacag
     7141 taaaacaagc agttatactg ctctgactca attcaagaca caacgtccaa acttcaatgg
     7201 ggacctggct caacttgtag cccttggagg aaataatatt ggaggtatag cgtggatagg
     7261 cggactatgt acggcatata aatatagcta tgccaatatc cagaactact acaattccgt
     7321 accggtttat tcatggacag ttgaagtaat gacgcatgag atcggccata accttggttc
     7381 tccacatacc catagttgtt catggccagg aggtgcactg gacaattgct accctgtgga
     7441 agggaattgt aatccgggcc cgccaccgac cactggtgga accatcatga gttactgtca
     7501 cctgacaaat tatggcatca atttcaacaa tggttttggg caaaagccag gtgacctgat
     7561 tcgtgccaag gtcaatgcca gttcatgtct tgggtattgt gatgatgggg ggggacctat
     7621 gtgcggagct ccgcaaagtg tgactatttc caacatcacc aacaattctg ccatggtatc
     7681 ctggacgacg ggactcaacg gcatttacta tatcgtggaa tacaaggtgt ctacctccaa
     7741 tacctggact attctacctc aacaaagttc tacatcatta cttttatcag gattaaattc
     7801 aggcacaacc taccatgtaa ggataaaaac catctgcagt gaaggtgaat ccgaatactc
     7861 cgctgtatat acctttacaa caggaaatgc atgtggaatt ccctatgggc ctttcgtgca
     7921 aaatatcaca gaaaccagtg cctatgtata ttggaaaccg gttcccgggg ccacttccta
     7981 tgatttcagg tttaaattga cgtccagcaa caattgggct accatcaata catcttcaac
     8041 aagcatttcc ctttcaggat taacgccggg tacttcatac gatgtaggtg tcagggcgag
     8101 atgcggtact gtgtacagtg actataccgg gacctataca ttcgtaaccc ttggtaatcc
     8161 tcccggtggc gggtccggtt attgtgactc tcatggaaca agctctgctt ttgactggat
     8221 tcaaaaatta actttcggtt catttacaaa tgaaagcggt aataacaacg gatatggcga
     8281 ctttttgaat caaaccataa gtgcaaagaa aaatacgatg gtaattattt cagttacccc
     8341 gggtatgatc ggtaactata ccgaattctg gactgtttgg atcgacttta accagaatga
     8401 aagctttacc gatccgggag aacaagtagc aacatttacc acaacaaata ccaatccgaa
     8461 ttacactaaa atctttatac cggataatgc ttccttagga aatacaagaa tgagaatcca
     8521 aatgaagaga ggatcttttt cgacttcctg cgaagcattc ccttatggcg aggtagaaga
     8581 ttacacgctc agtataaccc agcaattgaa aggaggcacc tcagagtaca gttcatcaaa
     8641 taatacgacc ctcttcccca acccattcaa cgagaacttt actttgaatt tcgaatcaga
     8701 gaatgccgaa gatgccagaa tcgaaataag cgatatcacc agcagaatca tattctcgca
     8761 tcctatgagt gtccaaaaag gattgaacag cttccgttta gaagatactt cctccatgga
     8821 acccggaata tacatggtac gtattatagg acaaaatatc aatgaaactc acaaagcaat
     8881 aaaagtttct aacagaaact aaaattttta agttaagatg tttagttggc gggccttcag
     8941 ggcctgcctt ttttatataa gacaaatcaa aaacgcgcaa ccagcaagta taattcaact
     9001 caagggtaat aagagatcta tcacccccca taagtattga actcttcatg taccaggctt
     9061 caaaccatac tgtttgggtt cagagcaatc tgtgcagtca tttgcatcgc tattaataaa
     9121 gctttcattt agtaaaaagg aagcgtatcc aatgaacgtc ccgaaaaaat caaagattat
     9181 ccaagagcta ataactcaat catcgacatg tatccgctcc gacaggatat ttcatccaaa
     9241 atagaatggt caatagtcgg aaataccatg agagtcaaag cgacctgatc gcaggaagac
     9301 cttgaaggtg caatcactgt catcattttc ttcttttggt tgatagatac gttctgtcag
     9361 aacccattcg tcccagttga tcataggaaa aaaagtatct cccctggcca ccatatgcac
     9421 ttcggtataa tacagtttgt cccacaaccc tatggtctgc ctgaatattt ctgctccacc
     9481 gatgataaac acttcttcat taccggtgcg agctgccaga tccatcgctt cttccacgga
     9541 atgtgcaatc aaagctcctg aggcgagata aaacatatct cgggttatga taatattctc
     9601 tcttttcggc aaggggcgtc cgattgactc aaaggtggta cgccccatta tgatcggatg
     9661 gtgtagtgtt tttttcttaa agtttttaaa gtcagatttc agatgccagg acatggctcc
     9721 tctatcaccg atgacgccgt tttctgcaac ggctacaata gctgaaacga tcatttttta
     9781 tctaattttg gtggcccaag gagattaagc cgtgtcattt ccttttctct ttccttcctg
     9841 aatggagctt caatgtagcc ccgaagatat ttctccacga tcaatgaagc gattggtgca
     9901 gcagactgag ctccgaaccc ggcattttct atcaacacag caatggcaat cttcggatta
     9961 tcctttggtg cgaatgccat gaaaacagcg tgatctctat agggtggatt ttgagaagtc
    10021 cctgtcttac cacaaagcgt gataccgggt ataggtgtgc tccagccgca ccacttactg
    10081 gctacagccg catccatgcc atcaattacg ggcttgaaaa acttcgcatc cacacctgac
    10141 atgtgctttt cgcgaaactg ctcagggatc tccatttcct tatttttaaa gcctttgatt
    10201 aaatggggaa cataatagta accccgattg gcaataacag ctgcaagatt tgcaatctgc
    10261 aaagctgaaa gttgtatctc accctgaccg atcccaataa acatcatata ggtagatctc
    10321 cagtctttgc catatatttt ttcataataa gcaggtgtcg gaatattgcc ttttgtctca
    10381 tgcatcatgt cggtaccaag cgggtgaccc aatccaaagt caaacagatg tcgatcgagc
    10441 tctgtaagac cttttgaagg tctcgtgaac ccgtacttgt caacggcatc cctaagtaca
    10501 gtgataaaat aggtgttaca tgaatactgg agtgcatgtg agacattgac agaagccggg
    10561 tgagcatgac atttgacttt acctccccta taatagtaag cgccatggca gctgattgaa
    10621 cgattcggtg taaggacacc catctgcatt gccgaaagag ctacaacagt cttaaatatc
    10681 gagccgggag gatacttagc ttgagcgatc ctgttgtaca atggtttgtt ataatcatta
    10741 atcaactcac caaaagcttt gccccgacct tgattaatag taagcagatt agggtcataa
    10801 gagggagaac tgaccgctgc aagaatttcg cctgtctgag gctcaatcgc taccactgac
    10861 cctcttttac cttgcatgat gctttctgca tactgttgca agtctaaatc gatggaagat
    10921 atcaggtcct gacctgagac cgggatggaa tcttgtgcgc cattacgata tgggcctacg
    10981 ggtctaccca tattatcctt catgatatac tggatgcctt ttctacccat cagcaaacgt
    11041 tcatattggc cttccatccc cgaaatgccg atatagtccc caagcccgta aaggcctccg
    11101 ctacttgcta tctgattctg gtcaacctcc cggatataac ctaaaacgtt ggcagcatta
    11161 tgatgaggat agcctcttgc gatacggagt tgtggaaaaa atccaggaaa ttcccataga
    11221 gattcctgaa atcgggtata ggtatacggt gatatttttt taaggaaagg aaaaggaata
    11281 gactttgaaa atcttttgct cgcccaatct tttgatagat tggcaataaa agtctctttg
    11341 tctattccaa gaatattgca gaatttggcc gtatccatat ttgggtcaat ggcattatag
    11401 gtaaccatca agtcatacat cggatcgtta ttgaccaaaa gcttgccgtt tctgtcatag
    11461 atgattcctc tggcaggata aacaacatgt ttttcaatgg cggtcgcatc agccctaact
    11521 tgaaatgaag tgtcaaacaa ctggatctgc gcagctttca tcacaagcac catggcactc
    11581 atgataaata ccatttgaat gatacgaatc ctactatccg gtttggcgac cattattcct
    11641 tcggattaaa aagatacata taaaccagaa taaaaatcat agaagcaata aagcttaaaa
    11701 atgtccttgt tattatttcc aggaagtaaa caaatgtaaa tttctgaacg gaaaaaagga
    11761 aaaacaaatg cagaagtaat aaaatggagg aatagaacaa aaaccaacga ttgccataag
    11821 caatgatggt tggagaagaa tcaacggtat atcctccacg gggctctaac cgtgacaaaa
    11881 caaacggcct gataaaccct gtaaacacac atgctgatgc atggacaccc ggcgtattat
    11941 aaaacatatc cacaagtata cccaatacaa aggcaatcac caaaatcagg gcacccggag
    12001 tcctcatcgg caataacatt atgatgaccg ggtaaatgat aatgctaata ttgtccagaa
    12061 caggatagta aattgtcaga ctcctgaata ccaatacctg taacagcaat ataaaaacaa
    12121 aacggacaag gtttcttgct attgttcctc ccatggtcac tatcttaata ttgcattatt
    12181 caacgagtca agctggggct tggttttatc aagaataacg tatacatagg tcagattgtt
    12241 aaagtcagtg gtcaacttca ccttcaaatc ataaaaactg ctccccggat caaaaccgac
    12301 attttccacc cgacctatat aaataccacg tggaaatgta gtactgtagc cactggttat
    12361 cacgctgtcg cccttcagga tatcggcatg cttaggaata tcagtcagat agacgtactc
    12421 ggtggaagtg ttgtcccaga ccaaagaacc gaagtagcca tttcgtttga ttgcggcact
    12481 aattttggtc tggctaccca acaaggaggc gacagtagca taatttcggg aagtctggcg
    12541 aac
//
LOCUS       DAOQMC010000043        12172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2001_length_12172_cov_4.772386, whole genome shotgun sequence.
ACCESSION   DAOQMC010000043 DAOQMC010000000
VERSION     DAOQMC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2001_length_12172_cov_4.772386"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKV31_03050"
     CDS             <1..336
                     /locus_tag="PKV31_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNB91861.1"
                     /translation="IPTGIITESTEARSQIKNREIALQRLYQLIMEKNRNEVQNAMAS
                     QRKTQVGTGDRSDKIRTYNFPQNRLTDHRINLTLYNLDSVMEGNIGDIIEGLRVNDNA
                     EKLKAIEGI"
     gene            complement(843..1085)
                     /locus_tag="PKV31_03055"
     CDS             complement(843..1085)
                     /locus_tag="PKV31_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91862.1"
                     /translation="MISRNEGHQLLIFKAFSLVFVCTGPLLGVPFQINDLSSYKDLGR
                     QSSLKNLEEVYMLRFVYRPAYPGGTRLFIPIVVAVN"
     gene            1444..2253
                     /locus_tag="PKV31_03060"
     CDS             1444..2253
                     /locus_tag="PKV31_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB91863.1"
                     /translation="MENQDNYLEINRRLWDAKTAYHLNSEFYDVKTFLDGRNSLTEIE
                     LALLGDISGRSVLHLQCHFGQDTISLSRMGASATGVDFSSKAIETARQLAQESGSTAQ
                     FICSDIYDLPNQMDKKFDLVFTSFGVIGWLPDLKKWAEVIAHFLKPGGKFIFAEFHPV
                     AWMFDNNFSSIQYNYFNVEAIREEAVGTYADRDAPIRNESITWNHSLSETLNALIESG
                     LEIKHFEEFDFSPFNCFNEMILSDDQKFRIRQLDNKIPLTFALTAVKKIRN"
     gene            complement(2259..2870)
                     /locus_tag="PKV31_03065"
     CDS             complement(2259..2870)
                     /locus_tag="PKV31_03065"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB91864.1"
                     /translation="MSKFIGISIFLVPLVFSILAFRTYDNGAYTIGDTVEDFTLTNYD
                     GSMISLSGYNNAKGLIIVFTGNQCPFDQAYESRLIDLDRKYRADGFILLAINPNDAGM
                     SAVDNLEAIRARAQQKSFTFPFLFDESGSLRRKFGVVRSPHAFVLDGARKIVYSGAID
                     DSVTEPEKIRSRYVEEAIIALKEGKSIVKPLTKPVGCTVKADF"
     gene            complement(2952..3509)
                     /locus_tag="PKV31_03070"
     CDS             complement(2952..3509)
                     /locus_tag="PKV31_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91865.1"
                     /translation="MRTNIYLIIDLLFFRLVVCAIFLFSMNNVMGQRYLQVEKHHKKV
                     KLKYTAGDDITFRLRQSDQWLTSVLTDIDFDNNILFFTNLTVPLDSINGIKSHKPLVA
                     PELGMAVLRSGLSGLGTSLLYTLAFQPSNAKEFLLFFGGVSASGLGLTQLSKKRRVFD
                     IGNKFNLRITDLNIYIPQSPPVPGG"
     gene            complement(3499..3879)
                     /gene="rnpA"
                     /locus_tag="PKV31_03075"
     CDS             complement(3499..3879)
                     /gene="rnpA"
                     /locus_tag="PKV31_03075"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNB91866.1"
                     /translation="MKRPFSFPKSQRLKNPLSISILFQSGDNVFSYPVKMVFSFEADP
                     VCHPSFKAAVSVPKKSFKKAVDRNRLKRHMREAIRLNQSLIPVREGHTLRFIIIYAAR
                     EVLPAQQIHKAVRYLFEKLNKDEN"
     gene            complement(3948..4103)
                     /gene="rpmH"
                     /locus_tag="PKV31_03080"
     CDS             complement(3948..4103)
                     /gene="rpmH"
                     /locus_tag="PKV31_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNB91867.1"
                     /translation="MKRTFQPSRRKRVNKHGFRARMSTKNGRRVLASRRAKGRHKLTV
                     SDEMKTK"
     gene            complement(4287..4940)
                     /locus_tag="PKV31_03085"
     CDS             complement(4287..4940)
                     /locus_tag="PKV31_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91868.1"
                     /translation="MSSTIIRYSDEELEEFRVLIEEKLSVARNELEFMKEQITEMNEN
                     ASEKQGGNWFDDSSIHTELEMLNNMVIRQQQFIHNLENALIRIQNKTYGICSVTGQLI
                     DKNRLKLVPHATKSVVAKEIENAEKAKQEAIARAEKEAAKASEKSGESADVKPKIITK
                     IVKRSNKPATKTAPKEEDLEDLDLDLDLEEEESDYDDTLINGMDMDDIADDQDYFDK"
     gene            complement(5093..6280)
                     /locus_tag="PKV31_03090"
     CDS             complement(5093..6280)
                     /locus_tag="PKV31_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNB91869.1"
                     /translation="MKRNFSIYLLLAFMLGGFCLNAQDDLINKIKGNQSANAKFEFTK
                     IIDLENTSIKDQGSSGTCWSYSTVSFLESEMARIGKKPVDLAEMFIVRNQYIQKAENY
                     VRMHGGLLYGQGGAAHDVIKIYGQFGIVPQNVYTGLNYGTTRNNFNELEAVLKGFLAP
                     LVGDNVKMLTPVWKTAFTRIVDTYLGEVPEKFMYEGKEYTPKTFADQVVGLKAEDYVE
                     FSSFTTSPFYKPTVLMVPDNWSFDMVYNVPIDDLTAIVDYALKNGYTITWGTDVSDRG
                     FSWKNGVAYVLPMDYDAMSEEQKKDIFNGPQPEGKVTQEERQRQFDNYQTTDDHGMQI
                     VGLYKDQNGREYYKVKNSWGQRNDFDGYLFVTKNFLKLKTTDLLLHKGGVPKDLLKKC
                     GIQ"
     gene            7236..8267
                     /locus_tag="PKV31_03095"
     CDS             7236..8267
                     /locus_tag="PKV31_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB91870.1"
                     /translation="MVKIKYIYRLSLLIVFLGFIACKAEKKEEVQSKISNDPNLMSLN
                     DFIIKNPGNSEAYFNRAKYFYDLKAYDEAIVDLASAMKIDSMKPKYYHLLADTYMDYY
                     QSRMALMTMQKASGMFPDSIHTLLKLTEYEIILQKYDAAMQTIRSILEKDKQNADAYL
                     LMGTVLEESGDNKRALAAFKKSTEMNPFLIDAWIKAGEVADKMGEKDAKKYFETAIRI
                     DTSNYSALYATAMHYQNKNDRPEAIKWLKKINLIHPNIPDPYLNIGILYNEMDSLQKA
                     LEFVDIAINLDKVYSNAYFVKGTILERQGNLKEAQKNYSQASTLNPKFTKAMEESERL
                     RKQLEAVKK"
     gene            complement(8279..10234)
                     /locus_tag="PKV31_03100"
     CDS             complement(8279..10234)
                     /locus_tag="PKV31_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNB91871.1"
                     /translation="MSQVNNLADTLKERNRGHQIFQRIAAWPGRIFSTELNPMYQLGG
                     LAVLMFVIACISGIYIFIFYNINPHQAYESVEAISNNYFNGWMRNIHRYSSDLLVVFI
                     LLHLIHTLFTSKFKRLISWISGVISFLVVITIGVTGFILIWDQKAKLTGYLTAKLFSG
                     LPIFDPSIMGAFLLNDLSVVGGFFKVALFGHIALSLITVIIIWIHVLRISKPKIFPPG
                     KLILYASIALGIIAILFPVKSDPPAQMTNLPAETTFDWFYYFGYYLMKVFSVGQNWAI
                     MIGSGLLLSILPFMLRRKDRSPVHIDLDKCDACNLCSYDCPYGAIDMLIYNGERKAIL
                     SPDKCVGCTICIGSCKEHAITHPNFPVFDFSPDPKQDLTVFSCSFFPEVTFPSELNTT
                     QYKVPCLGSVMVKDVQKIMDGGTTKVAMLGCEDCYYRYGKNWAVERLLKKRPPAYSRR
                     LDTSGIRLFTINQFSPDRLLEFCREQPDENTGEAKIMDYGRARPILSTLMLAAFFALF
                     IPFSSTKVRFFNPADKTLVVNFKYISTPIEYEQSTSTMKHMQSATPVVKKRSPVVLKI
                     TDASDGKLLFEQEYHPRGLRNDIAMFIYTQMNVDADLVDIRLRETAGSGKTLSINDVK
                     VAKGDGTFVILKEGKLVVASRESAGSQ"
     gene            complement(10209..10979)
                     /locus_tag="PKV31_03105"
     CDS             complement(10209..10979)
                     /locus_tag="PKV31_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB91872.1"
                     /translation="MMDKQIFILFIFLWLILSGFRMPEEERHLGRKIPDITIMKADGS
                     RSNLYSLLRGKPLVLTPIYTKCPSICGLISNGTKEAVTALGTLGRDFNMVSFSFDSTD
                     TPDNLSYYQQRWKMDGETWQTVTSDGKSIRSLLASIGYEYDRDTVLKQFNHPALLVVL
                     TPSGRISRFIYGVNPTKRDIDLAVMAAMAEQTRPGLFKGFYLRCFGYDPLTRTYKIDW
                     RFIISTSAGIIIIFFVTSIFLKTFIFSKPEHVSSEQSG"
     gene            complement(11043..>12172)
                     /locus_tag="PKV31_03110"
     CDS             complement(11043..>12172)
                     /locus_tag="PKV31_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB91873.1"
                     /translation="MTAAIIAVITLASGALIYIPTFLWSLGLIENIDPAMYKLIWWGL
                     GHSSQQINVAAMVSIWYMVSFLTVGGTSINEKVSRTAFVLYILFICLASAHHLLTDPG
                     VSSAWKVWNTSYAMYLAVLASMIHAFAVPSSYESAQRRWGYNKGMFEWLSKAPWSNPA
                     FSSTMLAIIGFGFIGGTTGVIFGMEQTNIIVHNTIAIPGHFKGTVVIGTTLTFMGITY
                     YLIPLIFRRKIVGMGMARWQPWLFFIGIALLSISMIVLGQLGVPRRHWDSTFSGGPFT
                     YNFNPAVDFFWTLMMLGGTIAFISVMMWIGIVVVSVFFGPKVNGPGDMQLTLSPLAPP
                     AKTHKGFEAPGTLLLTFIFLLVFLALFFLNWKWLAATWYVK"
BASE COUNT         3786 a         2620 c         2342 g         3424 t
ORIGIN      
        1 attcctaccg gcatcattac cgaaagtacc gaagctcgct cacagatcaa aaacagggag
       61 atcgccttgc aacgactgta tcagctgatc atggagaaga acagaaacga ggtacagaac
      121 gccatggctt cacagcgtaa aacccaggta ggaacaggtg acaggagcga taagattcga
      181 acttataatt ttcctcaaaa tagacttaca gatcatcgga tcaatctaac cctttacaac
      241 cttgattctg tcatggaagg caatatcggt gatatcatcg aaggcctaag ggttaacgac
      301 aatgctgaaa aactaaaggc catcgaaggg atttgatttt aatcccctac cccgcccctt
      361 tagccatacg cattcaggga gtatccggat tgcattattt gacgctatcc ttaagtgatc
      421 cggcttcttt catccttaaa tattcttcgg ttatagatca cagtggatca caaaaacagg
      481 aagggtggaa tcaaatcgct gtatggttta tgagtttttg atcaaataat aacgaatgta
      541 ccttcggcat cccggaacaa cggattgttt cactcccggg acagagcaga gctctggtag
      601 catagctgtc cggacaatta taccaaaaat ttataaacta ttatataaat catcttggta
      661 taacaacgat taccatcaac cagatctgaa aatccggctt ttctaaaatc cggggccaac
      721 ctattaaccc gggtaccggt aaattgaatt ctccggaaaa gcatttcaaa aaaagcagct
      781 taacagacaa aattaatgct caaaatcatg ggagtccatt ctatgacatt ttttgaagca
      841 aatcaattga cagctacaac aatagggata aaaagccggg ttccacccgg atatgcaggc
      901 ctatagacaa atcggagcat atatacttcc tctaaatttt ttaaggacga ttgccggccc
      961 aggtctttat acgaagataa gtcgttgatt tggaatggaa cgcccaataa gggtcctgta
     1021 cacacaaaaa ccagactgaa ggctttgaat attagtaact gatggccttc atttctggag
     1081 atcatgagcc agggttcatc caaatcaaag gatatgatga ttgaaacaat tacgatttgg
     1141 caaaggcaag tactgatgat ggcagatcaa acattttttg atggataacc aattaaatac
     1201 ttttctttgc aggactaatc acgataaaag atttatttcc atatactcag tgtaaatatg
     1261 cctttttcaa tcgatggttg tgatgtcatg aagtgtgagc gtaaaaaatc tcctatcagc
     1321 ttgtaatggc gcactttatt tcattcctaa actaaaaaaa ttaaatcctt cgggtaaaaa
     1381 acaaacgtat taattattga gccggtaagt tagtgctcag aaaaaaaatc aaataaagtc
     1441 attatggaaa atcaagataa ttatttggaa atcaaccgaa gattgtggga tgccaagacc
     1501 gcgtaccatc ttaactctga attctacgat gtcaagacat ttcttgatgg aaggaattcg
     1561 ctgactgaga ttgagctggc attgctggga gatataagcg gtaggagtgt attgcatctt
     1621 cagtgtcatt tcggtcagga taccatctca ttgagcagaa tgggggcttc tgctaccgga
     1681 gttgattttt cctccaaagc cattgaaaca gcaagacaat tggcacaaga gtcaggttcg
     1741 actgctcaat tcatttgttc agatatctat gatttgccca atcaaatgga caaaaagttt
     1801 gatcttgtat ttacgagttt cggcgtgatt ggatggctac ctgatcttaa aaaatgggct
     1861 gaagtcatag ctcatttttt gaagcctggc ggaaaattca tttttgccga atttcatccg
     1921 gttgcatgga tgtttgacaa taatttttca agtattcaat acaattattt taatgtggaa
     1981 gcaatccggg aagaagcagt cggcacctat gccgataggg acgcgcccat ccggaacgag
     2041 agtataacct ggaatcatag tttgagtgaa acactcaacg ctctgattga aagtggtctt
     2101 gaaatcaaac atttcgaaga atttgatttt tcgccattca attgcttcaa tgaaatgata
     2161 ttatcggatg atcaaaaatt ccgtattagg caactggaca acaaaatccc acttaccttt
     2221 gctttgactg ccgtaaaaaa aataagaaac taaggggttt aaaaatcagc tttgacagta
     2281 catccaacag gtttggtcaa cggtttgaca atactttttc cctctttcag agcaataatg
     2341 gcctcttcaa catatcgact cctgattttt tcaggttcag tgacactgtc atcaatggcg
     2401 ccggaataaa caattttacg ggcaccatcc agcacaaagg catgcggaga ccttactact
     2461 ccgaattttc ggcgtagact tccgctttca tcaaaaagaa aaggaaaggt aaatgatttc
     2521 tgttgtgctc tggcacggat cgcttcaagg ttgtccacag ctgacatacc cgcatcattc
     2581 ggattaatag ccaacagtat gaaaccatcg gcgcgatact ttctatccag gtcaattaat
     2641 ctgctctcat acgcctggtc aaaaggacac tgattgcctg taaaaacaat gataagcccc
     2701 ttagcattat tataaccaga tagtgaaatc atgctgccat cataattcgt aagggtgaaa
     2761 tcttccacag tatccccaat agtataagcg ccattgtcat aggtccggaa agccaaaatg
     2821 gaaaaaacca gcggaaccaa aaagatagat atgccgataa acttagacat cgaattaata
     2881 tttctatttt taacattaaa acccatgata actcaacaaa taaaactata atcaagttct
     2941 cgaaagaaaa tttacccccc gggaaccggc ggcgattgag gtatgtagat gttgagatcg
     3001 gtgatccgaa gattaaattt gtttccaata tcaaatactc ttctcttttt ggataactgt
     3061 gttaaaccaa ggccgctggc ggatacacct ccgaaaaaaa gcaggaattc cttagcattt
     3121 gatggttgaa aagcaagggt ataaagcaag gatgttccca ggcctgacaa gcctgaccgc
     3181 aatacagcca taccgagttc aggcgccacg agtggtttgt ggcttttgat accattgata
     3241 gagtccaacg gcacagtcag atttgtaaag aaaaggatat tattatcaaa gtcgatatcc
     3301 gtgagaactg aagtaagcca ttggtcgctt tgtcggaggc ggaatgtaat gtcatcccct
     3361 gctgtatatt tgagttttac ctttttatga tgcttttcaa cctgaagata cctttgcccc
     3421 attacattgt tcattgagaa aaggaaaata gcacaaacta ccaaccgaaa aaacagcaaa
     3481 tcaataatca agtatatgtt agttctcatc tttattcagt ttctcaaata aataacgcac
     3541 tgccttatgg atttgctgag ccgggaggac ctctctggca gcataaataa taatgaagcg
     3601 gagtgtatgg ccttctctaa ccggaatcag ggactgattc aggcgaatgg cttcacgcat
     3661 atgtcttttc agtctattcc tgtcaaccgc ttttttgaaa gatttctttg gcactgaaac
     3721 tgctgccttg aaggagggat ggcagacggg atctgcttca aaagaaaaca ccatcttgac
     3781 cgggtaggag aaaacgttgt cgcctgactg gaaaagaatc gaaatggaca aagggttttt
     3841 aagcctttga ctcttaggaa atgaaaatgg tcttttcact gaaaataaaa ataggctatt
     3901 gtgctgactt gcgcaatagc ctatcaatag tttttataag gaataactta ctttgtcttc
     3961 atctcgtcag aaacagtcaa tttatgtctt cctttagctc ttctgcttgc cagcactctt
     4021 ctaccatttt tagtgctcat tctggctctg aatccgtgct tatttactct tttacgcctt
     4081 gaaggctgga acgtccgttt catagaatat ggttattttt tcaaaaagtg ggacaaaggt
     4141 attaaataga ctttgattaa aaaaacttta aatccatttt tttattactt ttttaagcaa
     4201 cattgttccg gaggatgatc ggacgaacta ttcacaaagt aaaaagcccc acgaaatgta
     4261 gggcttttgt atgtgattgc tctctattat ttatcaaaat agtcctgatc atccgcgata
     4321 tcgtccatgt ccataccatt gatcaaagta tcatcatagt cgctttcttc ttcttccaaa
     4381 tcaagatcca agtccagatc ctccaaatct tcttctttag gcgcagtttt ggtagcaggc
     4441 ttattggatc tcttgacaat ctttgtaatt atcttcggtt tcacatcagc agactctcca
     4501 cttttctcag aagcctttgc tgcttctttc tcagctctgg ctatagcctc ctgcttggcc
     4561 ttttctgcat tttcgatctc tttggccact acagacttcg tagcatgagg aaccaacttc
     4621 agcctgtttt tatcgattag ttgaccggtc accgagcaga taccatacgt tttgttctgg
     4681 attcgtatca gggcattttc aagattatgt ataaactgct gctgacgaat caccatatta
     4741 ttcagcattt ccagttccgt atgaatactg gaatcatcaa accagttacc cccttgtttt
     4801 tcactggcat tttcattcat ttcagttatc tgctccttca tgaattcaag ttcatttctg
     4861 gcaactgata acttctcttc aatcaatact ctgaattctt caagctcctc atcagaatat
     4921 cgtataatgg tagatgacat aatgtttggt ttttaacaat ttattcaatc tcagtttcgg
     4981 ataaagacaa aaacgtattt tacgtcttat aagttcatta aattttcatt cttaattgaa
     5041 agtatcattt ttgataaata caacgaaaga tacacatatc attatattta ttctactgaa
     5101 taccgcattt cttaagtaaa tctttgggaa ctcccccttt atgcaaaagc aaatctgtgg
     5161 tttttagttt caaaaaattc ttggtaacaa aaagatatcc gtcaaaatca tttctctgtc
     5221 cccagctatt ctttacttta taatattccc tgccattctg gtctttgtat aaccctacta
     5281 tctgcatgcc atggtcatcg gtcgtttgat aattatcaaa ctgcctttga cgctcttctt
     5341 gtgtaacctt tccctcaggc tgcgggccat taaaaatatc ctttttctgt tcttcactca
     5401 ttgcatcata atccataggc aacacatatg ccacgccatt tttccatgaa aaaccccggt
     5461 cactgacatc agtaccccat gttatggtat atccattctt caaggcataa tcaacgatgg
     5521 ctgtcaagtc atcgataggc acattgtaca ccatgtcgaa gctccagttg tcaggaacca
     5581 ttaatacagt aggcttataa aaagggctcg tagtaaacga tgaaaattcg acataatcct
     5641 ccgccttcaa gcccacgacc tgatctgcaa aggttttggg agtatattcc ttaccttcat
     5701 acataaattt ctcaggaact tctcccaagt aagtatctac aattctggtg aaggccgtct
     5761 tccacaccgg agtaagcatt ttcacattgt caccaacaag aggagccaga aatcccttca
     5821 gtactgcttc cagttcattg aagttatttc tggttgtacc ataattcaaa cctgtatata
     5881 cattttgtgg tacaatacca aactggccgt atatcttgat aacgtcatgt gcagcacctc
     5941 cctgaccgta aagcagccca ccatgcatcc tcacataatt ttcagctttt tgaatgtact
     6001 gattgcgtac aataaacatt tctgccaagt caaccggctt tttacctatc ctggccattt
     6061 ccgactctaa aaaagaaacc gttgaataac tccaacatgt tccggatgaa ccctgatcct
     6121 taatggaagt attttccaga tcgataatct tagtaaattc aaatttggca ttggcacttt
     6181 ggttcccctt gatcttattg atcaggtcat cttgtgcatt taagcagaat ccccccaaca
     6241 tgaatgccag caaaaggtaa atggaaaaat ttctcttcat tataatgttt tataggttat
     6301 tatccgattg gacaaacgat aattttccga ataatcaaac tcacattttg aaaattcggt
     6361 gcaatattaa taagtataaa taaaagtaca gaaaaaatta caaacttaat tgttaatttc
     6421 tccattccat agtgatgtca gtagattgcc aacagagaaa ttctttaaaa ataacagcat
     6481 cgatttaaaa tttatgacat tttaggttca caataatcac tgaggttatg agcaatgatt
     6541 ctccggaaaa gtctgtttaa ccggggccgg taacaggcca tttcagggat tgttgctcaa
     6601 tgggtgctgc agatcaaccc agagacagga ttatgtctac atttgacctt ttcttgtcaa
     6661 aactaaggat gaatccacgt cggcacacaa gaaaaaaaat gttgaggcat ccataagcaa
     6721 cccgagatct caatatctta tgcattccta ctaagaatct caaaactgta ccaggcttca
     6781 tgtcattttt attccaggct ccgggacttt atttaaccat gttcattcct aaagcgatac
     6841 tccccatctt cacttgttct accaaaatca tcggttctct ttccatttat ttcaatgggt
     6901 tacaacgcca taacaaaatc tgatagaaac cttaaaacag gtatttacct gcgccttgtc
     6961 taaagcaggt aagacaaatc tgtattgcta aaccttccat ctagagtaaa ggtagttgcg
     7021 acaggtcagg ccattattga ctgatacgtt ccaataaagg cttgattcaa tctattctca
     7081 catttggagt gaaagattac aaaggcctga ataattacct tgacaataca acagccgggc
     7141 aatgtgtatt gcatatcgta ctaataatgg aaatattcac ctgaatccat ctatctttgc
     7201 acttccttat acgttattca ggaaaaaaat gcattatggt taaaataaaa tatatatacc
     7261 gattgtcctt actgatagtc tttcttggtt tcattgcctg taaagccgaa aaaaaagaag
     7321 aggtacaatc caagatatcc aatgacccaa atttgatgtc gctaaatgac ttcatcatca
     7381 agaaccccgg gaactctgaa gcctacttca atcgagccaa gtatttttac gacctgaaag
     7441 cctatgatga agccattgtg gatctggcat cagccatgaa gatcgacagc atgaagccca
     7501 aatactatca tctgcttgcc gatacttata tggattatta tcaatcccgt atggcactca
     7561 tgacgatgca aaaagcgtca ggcatgtttc cggattctat tcacacattg ctgaaactca
     7621 ccgaatacga aatcatcctc caaaagtatg atgccgctat gcaaaccatc agaagcatcc
     7681 ttgaaaagga taaacagaat gccgatgcat atcttttgat gggtactgtc cttgaagaat
     7741 caggcgataa caaacgggca ttggctgcat ttaagaaatc gactgaaatg aacccatttt
     7801 tgattgacgc atggataaaa gcaggtgaag tggcagataa gatgggtgaa aaggatgcaa
     7861 aaaaatactt cgaaacagcc attaggattg acacaagcaa ctattctgcc ctttatgcaa
     7921 cagccatgca ctatcaaaat aagaatgaca gacccgaagc cattaaatgg ttaaaaaaaa
     7981 tcaatcttat acatcccaat atcccggatc cctatctaaa tattggcatc ctgtataatg
     8041 aaatggattc tttgcaaaag gcccttgaat ttgtagacat cgctatcaac cttgacaagg
     8101 tatattccaa tgcttacttc gtaaagggta ccatactcga gcggcaagga aatctaaagg
     8161 aagctcaaaa gaattacagt caggcttcca cgctcaatcc taaatttacc aaagcaatgg
     8221 aagaaagtga acgcttacgg aagcaactcg aagcagtaaa aaaataatgg actaatcttt
     8281 attgcgatcc tgcactttcc ctgcttgcga ctaccagctt accttccttc agaattacaa
     8341 aggtaccatc gccctttgcc actttgacat cattgatgga aagtgtcttg cctgagcctg
     8401 cagtctccct tagccggata tccacaagat ctgcatcaac attcatctga gtatagataa
     8461 acatcgcaat atcattgcga agccctcttg gatgatattc ttgctcaaac aataacttac
     8521 cgtcggaggc atcggttatt ttcaatacta ccggacttcg ttttttcacc acaggagttg
     8581 cagattgcat atgcttcatg gtagaagtac tctgttcata ttcgattggg gtagaaatat
     8641 atttaaagtt gacaaccaac gttttatctg ccggattaaa aaaccggact tttgtactcg
     8701 agaatggaat aaacaaggca aagaaggctg caagcatcag tgttgagagt atcggtctgg
     8761 ccctcccata atccataatt ttggcttccc cggtattctc atccggctgt tctctgcaga
     8821 attccaataa tctgtcgggg ctaaactgat ttatagtaaa taatcttatc ccggaagtat
     8881 ccaaccttct ggagtatgcc ggaggtcttt ttttcaataa tcgctccacc gcccagtttt
     8941 taccgtatcg gtaatagcag tcttcacaac caagcatggc taccttggta gtgccaccat
     9001 ccatgatctt ttgcacatcc ttgaccatga cgcttccaag acaaggcacc ttatattgag
     9061 tagtgttgag ttctgaagga aatgtaactt cagggaagaa gctacaagag aacactgtca
     9121 aatcctgctt tggatccggt gaaaaatcaa agacaggaaa gttggggtgt gtaatggcgt
     9181 gttccttaca agagcctata cagattgtac agcctacaca tttatcagga gaaagaatcg
     9241 ctttcctttc gccattataa atcaacatat cgatcgcccc atacggacaa tcataggaac
     9301 atagattaca cgcatcgcat ttgtcaaggt cgatatgcac cggcgacctg tccttccttc
     9361 tgagcataaa tggcaatata ctcagcagca ggcccgaacc tatcatgatg gcccaattct
     9421 gacctacact gaataccttc atcaggtaat acccaaagta atagaaccag tcaaaagtag
     9481 tctcagcagg caaattggtc atctgggccg gtgggtcgct ctttacagga aacaggattg
     9541 caatgatgcc caatgcaatg gaagcataca aaatcaattt cccgggtgga aaaattttag
     9601 gcttcgaaat cctcagaaca tgaatccata taatgatgac cgtaattaaa gaaagagcaa
     9661 tatgtccaaa tagagctacc ttgaagaaac ctccgactac agaaagatca ttgagcaaga
     9721 aggcacccat gatggaaggg tcaaagatgg gaaggcccga aaaaagcttt gctgtcagat
     9781 aacctgtcaa tttagccttt tgatcccata ttaaaataaa gcccgttaca ccgatagtaa
     9841 tcactaccaa aaatgatatc acaccactta tccatgaaat aagccttttg aactttgaag
     9901 taaacaaagt atggatcagg tgtaataata taaatacaac caacaaatcg gatgagtatc
     9961 tgtggatatt acgcatccag ccattaaaat aattattgga aatcgcctcc acggactcat
    10021 aagcctggtg cggattaata ttataaaaaa taaatatata aatgcccgat atacaagcta
    10081 ttacaaacat aagcactgct aatccgccca gctgatacat gggattcagt tcggtggaaa
    10141 atatcctccc cggccaggca gctatccttt ggaagatctg atggcctcta ttccgctctt
    10201 ttaaagtatc agccagattg ttcacttgag acatgttcag gttttgagaa aataaaggtt
    10261 tttaaaaaga ttgatgtaac gaaaaaaatg atgataattc cggcggatgt agaaatgata
    10321 aagcgccagt caatcttata agtgcgagtc aatggatcgt atccaaaaca tcggagatag
    10381 aatcctttga acaggcccgg cctggtttgt tccgccatag cagccatgac agcaagatca
    10441 atgtcgcgtt tagtggggtt tactccatag ataaatcggg agattctgcc ggatggtgtc
    10501 agaaccacca acaaagccgg gtggttgaat tgtttcagga ctgtatccct atcatactca
    10561 tatccgatgg atgcgaggag gcttcggatc gacttcccgt cggacgtgac cgtctgccag
    10621 gtctcaccgt ccattttcca ccgttgttga taatagctca gattgtccgg agtatctgta
    10681 ctgtcaaagg aaaagctgac catattgaag tcccgcccca gcgtacccaa agctgtcaca
    10741 gcctctttcg taccattgct gatcagaccg caaatcgaag ggcacttggt atagattggg
    10801 gtaagcacta atggcttccc tcttagcagc gagtaaagat tggacctact gccatcagct
    10861 ttcatgatgg tgatatcggg aattttcctg ccaagatgtc tctcctcctc aggcattctg
    10921 aatccggaaa gaatcagcca tagaaaaata aaaagtatga agatctgttt atccatcatg
    10981 aaaaaccggc ggctttatgc gggattggca ttccggaggc cgccggagga gttgttgttg
    11041 tactatttta cataccacgt cgctgcaagc cacttccaat tgaggaagaa cagcgcgagg
    11101 aataccaata agaatatgaa ggtgagcaac agggtgcccg gtgcttcaaa tcctttgtgt
    11161 gttttggccg gtggtgctaa aggggacaga gtcagctgca tatccccggg gccattgacc
    11221 ttaggtccga agaaaacaga tactacgaca atgcctatcc acatcatgac agagataaag
    11281 gcaatggtgc ctcccagcat catcaatgtc cagaaaaagt ccactgccgg attgaaatta
    11341 tatgtaaatg gccctccgga gaacgttgaa tcccaatgtc ttcgtggcac tcccaattgt
    11401 ccaagcacaa tcattgatat ggaaagcaag gcgataccaa tgaagaataa ccatggttgc
    11461 cacctcgcca tgcccatacc gacaatcttc cttctgaaga tcaaaggtat aagataataa
    11521 gtgataccca taaaggtgag tgtcgtacct atgactacag tgcccttgaa atggcccgga
    11581 atggctatcg tattatgcac gataatattg gtctgttcca taccgaagat aacaccggtc
    11641 gtacccccaa tgaatccgaa tccaatgata gcgagcatag tggaggaaaa agccggattg
    11701 ctccacgggg cctttgacag ccattcaaac atgcctttgt tatatcccca ccgacgttgt
    11761 gctgactcgt aggaagatgg aacggcaaat gcatgaatca tggaagccaa aacagccaaa
    11821 tacatggcat aacttgtgtt ccataccttc caggcactac tgacgcccgg atcagtcaat
    11881 aagtggtgag cagatgccag gcagataaag aggatataaa gcacaaatgc agtccgggaa
    11941 accttttcat tgatggaagt gcctccaaca gtaagaaagg ataccatata ccagatggaa
    12001 accatggcag caacgttgat ctgttgggaa gagtgtccaa gaccccacca aatcaatttg
    12061 tacatagccg ggtcaatatt ttcgatcaat ccaagcgacc acaggaaggt tggaatatag
    12121 atcagtgcac cggaagccag cgtaatgact gcaataattg ctgctgtcat ca
//
LOCUS       DAOQMC010000044        11957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2049_length_11957_cov_4.839019, whole genome shotgun sequence.
ACCESSION   DAOQMC010000044 DAOQMC010000000
VERSION     DAOQMC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_11957_cov_4.839019"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKV31_03115"
     CDS             <1..343
                     /locus_tag="PKV31_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539494.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HNB91874.1"
                     /translation="VEPRIMGVGPVPASLRALEKAGLAISDMDIIEINEAFAAQVLAC
                     LDSLKISDNDPRVNPQGGAIALGHPLGMTGSRILLSAALQLQRTGKRFALVTMCIGVG
                     QGYAVIIERAD"
     gene            689..2281
                     /locus_tag="PKV31_03120"
     CDS             689..2281
                     /locus_tag="PKV31_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91875.1"
                     /translation="MEKYFNKPILIVALILVTLNVVNAQRDLKSINVLSEKIQDRYSV
                     FIKWKKSNFRNLFINKTDLFPTGFDGRLNGSRIKAFSLLEGEDELLQTVDSTIFYWSG
                     DRDNTEKILFPVMNSLDITINQLLSDPENEFIPPVPLTDSILSLEFEESIGAFVPDDR
                     LVQTRNNQDLTELQLNYEHDGFAWIFDEKAEFEYDNRGNMILAREYDFNGTKWLQDDD
                     QSLEYNSSNRLTATQKIKHDSTTGGVYGQFKHAWSYDDKGRLTEVLNFDWSDAINGWE
                     DDEKELYTYDDLGNLTKAEFFSWNDDSKTWEGTERHHLLYNLNNLKEFVRSENFIGNQ
                     WVEVQQIYYTYDDNNRVVTMEIFHQDSSQWVKSQKAELTYPDINQVDITVLEWDKDAG
                     VYENLIKNWVVYNDLRQPLRIRYQIWDEDKWTYFTFLPNSNFYYEDYGPSGHTSGKER
                     EAEFTVFPSPARDIITVKCENVRVENIRILDIKGRVVRSYQSRLHENIIAIPVSDLTP
                     GTYFVRLEGAGCSGIKPFVVQR"
     gene            complement(2596..3270)
                     /locus_tag="PKV31_03125"
     CDS             complement(2596..3270)
                     /locus_tag="PKV31_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91876.1"
                     /translation="MENTELIGIWKTQNEKLEKTLTINEILLKELINNKARTSLRSLI
                     KLKTAGIIAFVLYLLLLGNALAFAIAHYSSAWNYFIVSVAVIALVNVKGFADYIKHLV
                     WANNINYNGSVMQIQQQLSKLQLSIIDHARMMCLQIPFYTTFYLSNSWFPHEVGLGYI
                     LFQVSMTTTFVVFSYWLYKNHKPENLDKKWFRNLIAGSGGKSVMEAMQFYKELEEFSK
                     DETASL"
     gene            complement(3272..3784)
                     /locus_tag="PKV31_03130"
     CDS             complement(3272..3784)
                     /locus_tag="PKV31_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB91877.1"
                     /translation="MNRSEQSELFLSVVHAHKGIIYKVVNSYCKNPEDREDLVQEIII
                     QLWKSFGQYDDSFKHSTWMYRIALNVAISFYRKESSRKRISNPMTEGIINFPEQDDFN
                     DTETEVGMLRSMIAQLKDLDKALILLYLDEKNHKEISRIMGISETNVATKINRIKNHL
                     KQKFAQLKKN"
     gene            complement(3771..3977)
                     /locus_tag="PKV31_03135"
     CDS             complement(3771..3977)
                     /locus_tag="PKV31_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91878.1"
                     /translation="MNKMKKGVKANLAFAIIAISLFATGCGITVNRNNYYYYPYQSEN
                     KSIMKTTYTDYLVKSQAYKCNEQI"
     gene            complement(4308..4991)
                     /gene="aat"
                     /locus_tag="PKV31_03140"
     CDS             complement(4308..4991)
                     /gene="aat"
                     /locus_tag="PKV31_03140"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNB91879.1"
                     /translation="MAPDGLLGLSDELRYEDLKTAYRFGIFPVNNPSEPILWWSPDPR
                     WILLSGQVFVSKSMRKILRDRPWRATCDTDFELTMHRCAEGKGRKDRHPSWITGEYID
                     VYKMFFLDGRAHSFEVWDHSGVMIGGLYGVLTGSVFVGESMFSDVSNTSKYAFILACN
                     FMHELGIEVIDCQMHSEHLESLGAIPVHRDWFLNYLKWNGFEVSGLMGSWEERFRVFM
                     ESTYGNNAG"
     gene            complement(5073..5363)
                     /locus_tag="PKV31_03145"
     CDS             complement(5073..5363)
                     /locus_tag="PKV31_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNB91880.1"
                     /translation="MNYSTFSGFDFEEDVLLKEATTIDQQELIVYNDDYNTFDWVINC
                     FVEILRHTAEQAEQLAYIIHFKGKASVKIGSYYALKPFKEALLDKGLSVVIE"
     gene            complement(5516..7282)
                     /locus_tag="PKV31_03150"
     CDS             complement(5516..7282)
                     /locus_tag="PKV31_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91881.1"
                     /translation="MHQHSKIQAWTAEVIKAFTLRISYILVFSICCSISIYSQVIPIT
                     QNGLVQPLVDRLDMKAGFDPEYHSSHKYLTRGELRRLLLRFDTLAQPEYQDKENIQYL
                     VDENLEGEIYACRKRDSTVLTNFLPLWNSRPILKLFYKTPANFLQADQKDFYVRLNPV
                     LNFGYGRVSGEGAGTTFQNVRGVEMRGGIANRLWFYANLHETQEAYPAYVNDWVKTMG
                     AVPGAGFLKTYQSSLFKGLTGKDYMNAEGIIGFNIIPQIGLQFGHGRNFIGDGYRSLF
                     LSDFAANYLYLKLNTKVWIFDYQNIFAELNATGQTGGDKLIPKKYFANHHLSLNITPW
                     LNVGLFESVVFSRDNHFELQYLNPIIFYRTIEQLVGSPDNAMVGADMKVRLGRSIKLY
                     GQIMLDELIIKNMLKQNGWWGNKYGIQAGIQYYDVAGIDHLDMRLEYNMVRPYTYSER
                     DSASNYSHHFQPLAHPLGANFYEIICQVRYAPTSRWTIEPYCMFAVTGEDHGDINNGS
                     NILKPYTTRTGDFGIGLPNGDRADIVHAGIDVSYMIYHNLFVELSAGYRSKKSVLEAY
                     NTRETWISAGVRMNMAKRKMLF"
     gene            complement(7282..7890)
                     /locus_tag="PKV31_03155"
     CDS             complement(7282..7890)
                     /locus_tag="PKV31_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91882.1"
                     /translation="MSRKQLLFVCVLLAGMAVFIQCKNDTLPTPKPRSFPRVIYPEKV
                     YRQFDTSFCEFTFEYPAYATIIQDTAFFGEKPVDACWFNIYVPQFQSLIFCTYYPLGK
                     KNTFFKVLNDAYKLAGVHNKKANFIDEIPIDLPGKVYGTIFSIEGPVASTYQFFVTDS
                     TKHFLRGALYFNARTNQDSLAPVAEFMRKDVQHMIKTFKWRD"
     gene            8297..9271
                     /locus_tag="PKV31_03160"
     CDS             8297..9271
                     /locus_tag="PKV31_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB91883.1"
                     /translation="MAKPLDLIKAPIREELEMFEKHFRDAMKSNVALLDRIMFYIVRR
                     KGKQIRPIFIFLSAKAAGGCTDSTYNAASLIELLHTATLVHDDVVDESMERRGFFSIN
                     ALWKNKVAVLVGDYLLSQGLLLSVNNNEFELLRIVAKAVKDMSEGELLQIEKARRLDI
                     QEEIYFDIIRQKTASLIAAACEAGSTSAGVSKEVAKQYRLFGEKIGIAFQIKDDLFDY
                     GSKDVGKPLGIDIKEKKMTLPLIYALQNTDPSNKRRIINLIKNHSKKQKAVREVIEFV
                     SQSGGLQYAEQKMQEYCDSAISILRTLSTNDGAIALEKLIAYVTQREN"
     gene            complement(9401..10240)
                     /gene="panC"
                     /locus_tag="PKV31_03165"
     CDS             complement(9401..10240)
                     /gene="panC"
                     /locus_tag="PKV31_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNB91884.1"
                     /translation="MHIFTKKAGLKAYLDKERKKGKSIGFVPTMGALHEGHLSLIIGS
                     RKDCDVTVCSIFVNPTQFNNADDFRKYPRVVEADRDLLEKASCDVLFLPDVEEMYAGT
                     KPIRPDLDGLDTRLEGEFRPGHFDGVVQIVYLLLKAVKPDRLYMGLKDYQQQLIVGRM
                     IREMKLPVTLVAEPTMREKNGLAMSSRNMRLTPVQREEAAAIYAMLTHAAQSIKEGLD
                     IPEVLQKAESELADFDCKPEYIVLCDAETLLPLERYDPDRKAILLTAVWWGDVRLIDN
                     LVF"
     gene            complement(10257..10784)
                     /locus_tag="PKV31_03170"
     CDS             complement(10257..10784)
                     /locus_tag="PKV31_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HNB91885.1"
                     /translation="MEKELVNRIANSPLITLDMEKHAPQWNYALFDIKDYLFHGLILK
                     EKDFRQALKELDWTQYEGKDLLVFCSNDAIIPMWAYMLIVAQASPYASNVIQEDEAAY
                     CSRIWKETIDTLDLSPYEGKKMIVKGCSDRPVPPSAYTTITGRLIKIAESVMFGEPCS
                     TVPVFKSKSVQARDK"
     gene            complement(10831..11394)
                     /gene="scpB"
                     /locus_tag="PKV31_03175"
     CDS             complement(10831..11394)
                     /gene="scpB"
                     /locus_tag="PKV31_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNB91886.1"
                     /translation="MNNLEQHIEVIIFTASEPVTAEFIGEMVSQIHGRDIGRDVVVGA
                     VEKINQRYESIHSVFKIFNIAGGYQLLTKKEYDPVIAHVQSNLSKKKLSTSALETLSV
                     IVYRQPVTKAVIESIRGVNSDYALQKLLEKGLVKIIGRSEDVGRPLLYAAGDAFLEHF
                     GLNDLNELPKLREFEEPEEAIGKPDTE"
     gene            complement(11391..>11957)
                     /locus_tag="PKV31_03180"
     CDS             complement(11391..>11957)
                     /locus_tag="PKV31_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HNB91887.1"
                     /translation="DNPNVFADEGLVISGGNFHAQPLALASDYLSIAVAELGSISERR
                     LYLLVSGQRGLSPFLVTKPGLQSGFMIAQYTAASIVSLNKQLASPSCVDSIVSSNGQE
                     DHVSMAANAGVKLWQIVNNVATVLSIEWMAACQAVEMKQYSISPVLKNLLDEYRKYVP
                     FVDEDRVLHNDFIRTQEFFDDIKWFKKS"
BASE COUNT         3361 a         2691 c         2552 g         3353 t
ORIGIN      
        1 agtggagccc cgcatcatgg gtgtcggacc tgtgcccgca agccttcggg cattggagaa
       61 ggccggcctg gcgatttcag acatggatat catcgagatt aatgaagcct ttgccgcaca
      121 ggttcttgcc tgcctcgata gtcttaagat cagtgacaac gaccccagag tcaatccgca
      181 aggaggtgcc atagcactcg gacatccgct tggaatgacc ggaagccgta tcttgctgtc
      241 tgcggcatta cagctccaac ggacaggaaa acgctttgca ttggtaacca tgtgcatcgg
      301 tgtaggtcag ggctatgcgg tgattatcga gcgcgctgat tgatccccaa ttcagcaata
      361 atcgatgttg tgatctgtca tcatgtatag atgaccttaa agaatcaaac caagaatgcc
      421 attttgttat ggacatatcg aagatataac ctaccctaca agccatttcc ggctcaggac
      481 tttcacgttt attatagaat aaataacttg tgaaaatttt taatttcaat gatttaagtt
      541 aagtaaataa caagatatag gcggaacttt aattttatta tttacctttt aatagataag
      601 caatcccgat taattacccg gattatttta atcattcaaa taactctaag caaataaaaa
      661 attcattttt cattttaaat agaatattat ggaaaagtat ttcaacaaac caatattgat
      721 tgtcgctttg attttggtga ccttaaacgt ggttaatgcg cagcgtgacc tcaaatccat
      781 caatgttttg tcggagaaga ttcaggaccg gtatagcgtt tttatcaagt ggaaaaaaag
      841 caatttccgc aatcttttca taaacaaaac cgacctgttt ccaaccgggt ttgatggccg
      901 actaaatgga tcaagaatta aggccttttc tttactagaa ggagaggacg aattgctgca
      961 aaccgttgat tctactatat tttactggag tggtgacagg gacaacacag agaagatcct
     1021 gtttccggtg atgaattctc ttgatataac cattaaccaa ctactttctg accctgaaaa
     1081 cgaattcatt cctcctgtgc ctttgaccga ctctatcctc tcgctggaat ttgaggaatc
     1141 aattggggcc tttgttccgg atgacaggtt ggttcagacg aggaataatc aggatttaac
     1201 agagcttcaa cttaattatg aacacgatgg ttttgcctgg attttcgatg aaaaggctga
     1261 gtttgaatat gacaatcggg gaaatatgat tctagccaga gaatatgact ttaacggaac
     1321 aaaatggctg caagatgacg atcagtcact ggaatataat tcatccaaca ggctcactgc
     1381 tacacaaaaa atcaaacacg attcaacaac aggaggagtg tacggtcagt ttaagcatgc
     1441 ctggagttat gatgataagg ggcgtttgac agaagtcctg aattttgatt ggagtgatgc
     1501 aatcaatggt tgggaagacg atgaaaagga actgtacacc tatgatgatt tgggcaatct
     1561 tactaaagca gaattcttct catggaatga cgattccaaa acatgggagg gaacagaaag
     1621 acatcattta ttgtacaatt tgaataacct gaaagaattt gtccggtctg aaaatttcat
     1681 cggtaatcaa tgggtagagg tacagcagat ttactatact tatgatgata ataacagggt
     1741 tgttactatg gaaattttcc atcaggacag ctctcaatgg gttaaatcac aaaaagcaga
     1801 attgacatat ccggatatta atcaggtgga cattaccgtc cttgaatggg acaaagatgc
     1861 cggagtgtac gaaaacctaa ttaaaaattg ggtcgtctat aacgatttga gacagccgtt
     1921 acgaattcgg tatcaaatct gggatgaaga taagtggact tatttcacat ttttgccaaa
     1981 ctctaatttt tattatgaag attatggtcc atccggccat acttctggta aggagagaga
     2041 agctgaattt accgtatttc caagtccggc cagggatata attaccgtga aatgcgagaa
     2101 tgtccgggtt gaaaatatcc gtattttaga tatcaaaggc cgggttgtta gatcatacca
     2161 atctcgtctt catgaaaata tcattgccat tccggtatcc gacctgacac ctgggaccta
     2221 tttcgtccga ctggaggggg caggatgttc cgggatcaag ccatttgtcg tgcaacgatg
     2281 atataatacc gaggttacag tcgtatagac caatttcatt gatttatacg actgtaacct
     2341 cggcattccg gaaccccgaa ttgtttcact cccgggaaag agcagagctc tagtagcata
     2401 gctgtccgga caagtatacc aaaaatttat ggacttttat ataaatcatc ttggtataac
     2461 atccttgaaa tggaaatcat cagagcttga tgctttccat ttctgttgta taacagatag
     2521 gaacaggcct cccgacaatt gcaacggcac catggcagca cgtcatgaag ctacccggag
     2581 caggatatct gctcattaaa gggatgcagt ctcatccttg ctaaattctt ccaattcttt
     2641 ataaaactgc attgcctcca tgactgactt tccaccggac cccgcaataa ggtttctaaa
     2701 ccatttctta tcaagattct ccggtttgtg atttttgtac aaccaatagc taaacacaac
     2761 aaaggttgta gtcatggaca cctgaaaaag tatatatccc aggcccacct catgtggaaa
     2821 ccagctattg ctcagataaa aggtagtgta aaaaggaatt tgaagacaca tcattctggc
     2881 atgatcaatt atcgataatt gtagtttgga caattgctgt tgtatctgca tgacacttcc
     2941 attgtagttg atattgttgg cccatacaag atgtttaata tagtctgcaa agcccttgac
     3001 attgaccaag gcaattacag caacggaaac aatgaaataa ttccacgccg aagaataatg
     3061 ggcgattgca aaggccagtg cattccctaa aagcaacaga tagagtacaa aggcaatgat
     3121 gcctgctgtt ttcagtttaa tcaatgatct taaagaagtc ctggccttat tgttgatgag
     3181 ttctttcaac aaaatctcat tgatggtaag cgtcttttct aatttttcgt tttgggtttt
     3241 ccaaatgccg attagttctg tattttccat tttaattctt tttaagttga gcaaattttt
     3301 gttttaaatg atttttgatg cggttgatct ttgtggcgac attcgtttcc gatattccca
     3361 taatacggga tatctcttta tggttttttt catccagata gagtaggatc aatgctttgt
     3421 caaggtcttt gagctgagca atcatgctcc tcaacatacc tacttccgtt tctgtatcgt
     3481 taaaatcatc ctgctccgga aagttgatga ttccttccgt catcggattg gatattctct
     3541 tcctgctact ctctttccga taaaaagaga tagcgacatt cagggcaatt cggtacatcc
     3601 aggtggaatg tttaaaactg tcatcatatt gaccaaatga cttccatagt tggataatga
     3661 tctcctgaac caggtcttca cgatcttccg gatttttaca atacgagttg acgaccttgt
     3721 agataatacc tttatgggca tgaacaacag aaagaaagag ttcggattgc tcagatctgt
     3781 tcattacact tgtatgcttg tgatttgaca agatagtcag tataggttgt tttcatgatt
     3841 gacttgttct cgctttggta aggataataa tagtaattat ttctgtttac tgtgattcca
     3901 catcctgtag caaataacga tattgcgatt attgcaaatg ccaagttggc ttttacacct
     3961 ttcttcattt tattcattga ttagctgttc aaatttttat ttcctgatta ttcgcatcag
     4021 catcgtaaat atcacatttc gggaattttt ttttggggca ttataattga ccggggtaga
     4081 tattttgtaa ctttttcgtt ctaatccagg caattattac caatgcaatc cccatcccac
     4141 ataccttcta aatggtagtg atggttgaat aaatagtcaa aaaaaagtaa tcagtactgt
     4201 ttttagctga cagctattct atccggatgt aggaagatgg atggtcatag cgatcttgga
     4261 ccaatcattg tttttaccgg gatagataat cttcatgggt taagaattca tcctgcatta
     4321 ttcccgtacg tactctccat gaacacgcgg aatctttctt cccagctacc catcaggccc
     4381 gacacttcga aaccattcca tttcagatag ttaagaaacc aatcccggtg tacaggaatg
     4441 gcacccaggc tctccagatg ctcggagtgc atctggcagt caatcacctc gatgcccaac
     4501 tcatgcataa agttacaagc caggataaag gcgtatttgc tggtattgga cacatcggaa
     4561 aacatgcttt cgccgacaaa gacacttccc gtcaatacac catacaagcc gcctatcata
     4621 acccctgaat gatcccagac ttcgaagcta tgtgctctcc cgtccagaaa aaacattttg
     4681 tatacgtcaa tatattcacc ggtaatccag gaaggatgcc tgtctttacg tcctttccct
     4741 tctgcgcacc tatgcattgt gagttcgaag tccgtgtcgc aggtggcccg ccatggcctg
     4801 tcccgaagaa ttttcctcat ggacttactt acgaaaacct gtcccgaaag taagatccat
     4861 cgcgggtcag ggctccacca cagtataggt tcgctgggat tgttgaccgg gaaaatacca
     4921 aatcgatagg cagttttcag gtcttcgtat ctcaactcgt ctgacaatcc caataaacca
     4981 tcgggagcca caacagacgg atgcctgaaa gttgagtcgg aaacgaaaaa attttcagaa
     5041 acgcccaagg aaaatcgtcc ggaacccgga ttttattcta ttacgacaga aaggcctttg
     5101 tccaaaagag cttccttgaa tggcttgagg gcataataag agccgatctt gaccgatgcc
     5161 ttacccttga agtgtatgat ataagcgagt tgctctgcct gttctgcggt atgacgtagt
     5221 atctccacaa aacagttgat aacccagtca aatgtattgt aatcgtcatt atagactatc
     5281 agttcctgtt ggtcaatagt tgttgcttcc ttcaacagga catcttcttc aaaatcgaag
     5341 cctgaaaatg tggaatagtt catatagtgt caaaaattgt caggattatt attgattctt
     5401 ccgttatcgg aaatattcaa aggtgcaaaa ttaatgttta tatcaccaaa gttaataaaa
     5461 ttttaactta aataacaaat gaattttatc ctaatgtatt ggagactcat attgttcaga
     5521 acaacatctt acgctttgcc atattcatgc ggacacctgc ggatatccat gtttcccttg
     5581 tattataagc ctccaacaca ctctttttgc tcctatagcc ggctgacaac tcgacaaaca
     5641 gattgtggta aatcatatac gacacatcaa tgcctgcgtg gacgatatct gcccgatcgc
     5701 cgtttggcag ccctatcccg aaatctccgg ttcgtgtcgt gtatggttta agaatattgc
     5761 tcccattatt gatatctcca tggtcttccc cggtaactgc aaacatacag taaggttcga
     5821 tggtccagcg tgacgttggc gcataacgca cttgacagat gatttcgtaa aaattggctc
     5881 ccaagggatg cgccagtggt tggaaatggt gtgaataatt agaagcactg tcgcgttccg
     5941 aataagtata tggcctgacc atattgtatt ccagcctcat atcaaggtgg tcgatgccgg
     6001 cgacatcata atactgtatg ccggcctgta tgccgtattt gttgccccac cagccatttt
     6061 gcttcagcat attcttgatg attagctcgt ccagcattat ctgaccgtac aacttgatcg
     6121 acctgcctaa ccggaccttc atatcggcac cgaccatggc gttgtcaggg ctgcccacta
     6181 attgttcgat tgtacggtag aagatgatcg gattgagata ttgaagctcg aaatgattgt
     6241 ccctgctgaa tacgacactt tcaaagagac cgacattgag ccatggtgtg atgttgaggc
     6301 tcagatggtg attggcgaag tatttcttgg ggatgagctt gtcgcctccg gtctgtccgg
     6361 tggcattcaa ttcggcaaag atgttctgat agtcaaagat ccacaccttg gtattcagtt
     6421 tcagataaag gtagttggcg gcaaagtcgg aaagaaaaag cgaacggtac ccatcaccga
     6481 taaagttgcg tccgtggcca aattggagtc caatttgcgg gatgatatta aagccgatga
     6541 tcccctccgc attcatgtag tctttacctg tgagaccttt gaagagagag gattgatagg
     6601 tcttcaggaa gccggctccc ggcacggcac ccatagtttt cacccagtca ttgacataag
     6661 ccggataagc ttcctgggtc tcatgaagat tggcataaaa ccacagtcgg ttggctattc
     6721 ctccccgcat ctctactcct cgtacatttt gaaaagttgt gccggcgcct tctcccgaaa
     6781 ccctgccata gccaaagtta agcaccggat tgagtctcac ataaaaatcc ttttggtccg
     6841 cctggaggaa atttgccggt gtcttgtaga agagcttcag aatcggtctg gaattccaca
     6901 gtggcagaaa gttggtcaag acagtcgaat ctcttttgcg gcatgcatat atttctcctt
     6961 ccagattttc atccacgaga tattggatat tctccttgtc ctgatattcc ggttgcgcca
     7021 acgtatcaaa gcgcaacaac agacgcctca gctcaccccg cgtcaggtat ttatgggatg
     7081 aatgatactc cggatcaaaa ccggccttca tatccagacg atcgaccagg ggctgcacaa
     7141 ggccattttg tgtaatggga ataacctgtg agtatatact tatagaacag caaatgctaa
     7201 aaaccaagat ataagatatc cgcagcgtaa aggctttaat tacttcggca gtccatgcct
     7261 gaattttact atgttgatgc atcaatctct ccatttgaat gtcttgatca tgtgctgcac
     7321 atcctttcgc atgaattccg ccaccggagc aagggaatcc tgattggtgc gggcattgaa
     7381 gtaaagagcc ccccgcagga agtgtttcgt actatcggta acaaaaaact ggtaagtaga
     7441 tgctaccgga ccctcaattg aaaaaatggt gccatatacc ttacccggca agtcaatggg
     7501 tatctcatcg ataaaattgg cttttttgtt gtgcactcct gctaatttat acgcatcatt
     7561 aagcaccttg aagaaagtat tcttcttacc caggggataa taggtgcaaa agatgagact
     7621 ctgaaattgc ggcacataga tgttgaacca gcaagcatcg accggttttt caccaaaaaa
     7681 tgctgtatcc tgaatgattg tggcatatgc cgggtattcg aaagtaaatt cacaaaaaga
     7741 agtgtcaaac tgcctgtaca ccttctccgg atatatgacc cgcggaaagg agcgtggctt
     7801 gggtgtaggt agcgtatcgt tcttgcattg tataaataca gccattccgg ccaacaggac
     7861 gcacacgaat aacagttgtt tccgactcat atctttttgt gaaattctat ctaaacagga
     7921 tcatcgacat cataatcgca acaaaagtaa taaaactatg gatttggatg agaatggatt
     7981 atggccgatc gtcagatacc tcagtcctat cttgtataaa acaacaggac catacatatg
     8041 ttggaccttg tggcacaggg aaatgatccc aaccctgttt tgagtaacat tgtaagagcc
     8101 taacatccaa tgggcaaggg cgtcaagggt caaaggccgg tctttacctt gttttttaag
     8161 cgggtttagg aagccatacg gacatctact attcggccgt aagcggagat ccggaataga
     8221 agtcgatatg gagaaaaaga aaactgccaa ggcatatgca tagtctccca aaatagcata
     8281 catttgcaag cagacaatgg caaaaccact tgatcttata aaagctccca tccgtgaaga
     8341 gctggaaatg ttcgaaaaac atttcagaga tgccatgaag agcaatgtag ctcttctgga
     8401 caggatcatg ttttacatcg tccgtcgaaa aggaaagcag atcagaccga ttttcatatt
     8461 tctcagtgca aaggcagcag gtggatgtac tgattcgacc tacaatgcag catctctgat
     8521 cgaactacta cacacggcaa cgttggtgca cgatgatgtc gtagatgaaa gcatggaacg
     8581 ccgtggattt ttcagtataa atgccttatg gaaaaacaag gttgctgttc ttgttggcga
     8641 ttacctgcta tctcagggac tgttactttc ggtcaataat aatgaattcg aacttcttcg
     8701 aattgtggcc aaagcagtga aggatatgag tgagggcgaa ttgctacaga tagagaaggc
     8761 gcgccgactg gacatccagg aggaaattta ctttgacatc atccgtcaaa aaactgcttc
     8821 cctcattgca gctgcctgcg aagccggctc cacttccgca ggcgtatcaa aggaagtcgc
     8881 caagcaatat cgattatttg gtgaaaaaat tggtatcgcc ttccagatca aggacgactt
     8941 gttcgattat ggatcgaagg atgtaggcaa gccattaggt atcgacatca aagagaagaa
     9001 gatgaccctt cctcttatct atgcgctgca gaatactgac ccttccaaca aacgtcgcat
     9061 catcaatctt atcaagaacc acagtaaaaa gcagaaggcc gtccgggaag tcattgaatt
     9121 cgtaagccaa tccggtggtc tgcaatacgc tgaacaaaag atgcaggaat attgcgattc
     9181 cgctatttca attctcagga cactatccac caatgatgga gccattgcct tagaaaagct
     9241 gatcgcgtat gtcacccagc gggaaaattg atcgttaccc aaagcccgat gaaaggaact
     9301 cttgaaggct ccgtgcccgc ctgacttatt ggccctcaat ctatttataa tctaaaggcc
     9361 ttcaccttct tccgattccc ttacacggat ttacccctcc tcaaaataca agattgtcta
     9421 tcaaccgcac atcaccccac caaaccgctg tgagcaggat cgccttcctg tccggatcat
     9481 aacgctccaa tggtaacaaa gtctccgcat cacaaaggac gatgtattcc ggcttacaat
     9541 cgaaatccgc caattcactt tctgctttct gcaatacttc gggaatatcc aggccctctt
     9601 tgatggattg agccgcatgg gtaagcatcg cgtatattgc ggctgcttct tccctttgaa
     9661 ccggtgtaag acgcatattc ctgctactca tggccagacc gtttttttcc cgcattgtag
     9721 gttcagcgac caacgtcact ggcaacttca tctcccggat catccgcccg acgatgagct
     9781 gctgctgata atccttcagt cccatataaa gtcggtcagg cttcaccgct tttagcagga
     9841 gatagacgat ctgcactaca ccgtcgaaat gtcccggacg gaattctcct tccagccttg
     9901 tgtccaggcc atccaggtca ggacgaatgg gcttggtacc cgcgtacatc tcctctacat
     9961 ccggtaggaa aagcacatca caggatgcct tttccagcaa atccctgtcg gcctcgacta
    10021 cccgcggata tttcctgaaa tcatcggcat tgttgaattg ggtaggattg acgaagatgc
    10081 tgcacacagt gacatcacag tcctttcggc ttccgatgat cagcgaaaga tggccttcgt
    10141 gcagggctcc catagtcggg acaaagccaa tagacttacc ttttttgcgt tccttatcca
    10201 gataggcctt cagaccggct ttctttgtaa aaatatgcat agcgagaggt ggatttttat
    10261 ttatcccggg cctgaacaga cttgctttta aatacaggaa cggtactgca tggttctccg
    10321 aacatcaccg attctgcaat cttaatcagt ctgccggtta tcgtggtata tgcagaaggt
    10381 ggcaccggcc ggtcactgca acccttgaca atcatcttct ttccctcata gggactcagg
    10441 tccagagtat caatcgtctc cttccaaatc cgggagcaat aggcagcttc atcctcctga
    10501 atgacattgc ttgcataagg cgatgcttgt gccacaatca acatataggc ccacatcggg
    10561 atgatggcgt cattggagca gaacaccagc aaatccttgc cctcatactg cgtccagtcc
    10621 aactccttca aggcctgacg aaaatccttc tccttcagaa taagcccgtg aaaaagatag
    10681 tctttaatat caaacaaggc gtaattccac tgcggggcat gtttttccat atccagcgtg
    10741 ataagcggac tgttggcaat tcgattgact aattcctttt ccattcgttc atgttgtgtt
    10801 ttttaataac agctattcag gtgaagtggt ttactccgta tccggctttc cgatggcctc
    10861 ttcgggttct tcaaattcac ggagcttagg cagttcgttc aggtcattca aaccgaaatg
    10921 ttccagaaaa gcatcaccgg cagcataaag gagcggcctg ccaacatcct cactgcgccc
    10981 gatgatcttg accagacctt tctccaggag tttttgcaag gcatagtcgc tatttacgcc
    11041 gcggatgctt tcgatcactg ccttggtcac aggttgtcga tatacgatga cggaaagggt
    11101 ctctaatgcg gaagtggaaa gcttcttctt gcttaagttg ctttgtacat gggcgatgac
    11161 cggatcatat tcctttttgg tgagcaattg gtaaccaccg gcgatgttaa atattttaaa
    11221 aaccgagtgg atcgactcgt agcgctgatt gatcttttca accgctccga ccacaacatc
    11281 gcgtccgatg tccctgccat gaatctggct gaccatctcc ccgatgaact cagcggtcac
    11341 cggctccgat gctgtaaata tgattacttc aatatgttgt tcgaggttgt tcaagacttc
    11401 ttaaaccatt ttatatcgtc aaaaaattcc tgagtccgga taaaatcgtt gtgcaatacc
    11461 cgatcttcgt ccacgaaagg tacgtatttc cggtattcat ccaacaggtt ttttaaaaca
    11521 ggactgatgc tatactgctt catctccact gcctgacagg ctgccatcca ttcaatcgac
    11581 aggacggtag cgacattgtt gacaatctgc caaagcttta cgcctgcatt ggccgccatg
    11641 ctcacatggt cttcctgacc attgctcgaa acgatactgt ccacgcagga tgggcttgcc
    11701 agctgtttgt tgaggctcac gatggatgcc gctgtatatt gagcgatcat gaaaccagac
    11761 tgcaagcccg gtttggttac caaaaatgga gaaaggccgc gctgtccgga caccaatagg
    11821 taaagccggc gctcggaaat actacccagt tcggccacgg ctatcgacag ataatcgctt
    11881 gccagagcca ggggttgcgc atggaaattg ccacccgaaa tgacaagacc ttcatcagca
    11941 aagacattgg gattatc
//
LOCUS       DAOQMC010000045        11838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2079_length_11838_cov_5.641772, whole genome shotgun sequence.
ACCESSION   DAOQMC010000045 DAOQMC010000000
VERSION     DAOQMC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2079_length_11838_cov_5.641772"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKV31_03185"
     CDS             <1..499
                     /locus_tag="PKV31_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HNB91888.1"
                     /translation="AALKKYAVARHPSFGKIYAFEVNAYGSANLMDDANVPSLLSLPY
                     LDTVSLKDKNYRNTRKYILSNENPFFFKGKAGEGIGGPHTGMDMIWPLGITMRGLTSS
                     DDHEIRFCIRQLLATHGGTGFMHESFHKDDATNFTRKWFAWSNTLFGEFLWKIYREKP
                     ALLDV"
     gene            complement(934..2841)
                     /gene="acs"
                     /locus_tag="PKV31_03190"
     CDS             complement(934..2841)
                     /gene="acs"
                     /locus_tag="PKV31_03190"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNB91889.1"
                     /translation="MGKYKIKNLEKYFKEYRKSIKDPTGFWEKIAAENFDWHSRWNKV
                     LDFDMKEARFEWFRNAKLNITQNCIDRHLQTQGDKTAIIFEPNDVNEPAQHITYRELY
                     ARVAKMANVLTDQGIRKGDRVCIYLPMIPELAVSLLACARIGAVHSVVFAGFSASALA
                     ARINDCDCKTVITSDGSFRGNKTIDLKHIVDEALHQCPGVERVLVVRRTGGDVVMKKG
                     RDQWLQPLLDEAAALHVAEVVDAEDPLFILYTSGSTGKPKGMLHTTAGYMVFMGYTFK
                     NVFGYKKKDVYWCTADIGWVTGHSYIVYGPLLNGATTLIFEGIPSYPDYGRFWQVIEK
                     HKVNIFYTAPTAIRSLAKESLDFVQRQDLSSLKVIGSVGEPINEEAWHWYNENIGHRR
                     CPLVDTWWQTETGGILISPLAFVTPTKPSHATLPLPGIQPVLMDELSNEIHGNQSGGN
                     LCIKYPWPGMARTIYGDHQRYKDTYFSTYPGKYFTGDGALRDETGYYRITGRVDDIII
                     VSGHNLGTAPIEDVINLHDDVAESAIVGFPHDIKGNALYGFVILADEAKGKKYDILTA
                     EINALIAEQIGPIAKLDKIQFVSGLPKTRSGKIMRRILRKIAENDLNNLGDTSTLLNP
                     EVVEEIKDNRI"
     gene            complement(2952..3578)
                     /locus_tag="PKV31_03195"
     CDS             complement(2952..3578)
                     /locus_tag="PKV31_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91890.1"
                     /translation="MICIIDTLPAQSESPSSATGQAALLKKGFLIVRLPSFDKQEKYY
                     NAIIGDSLSGKKERERAQAQLHALTTRRDGLIRVLRHTIDSVYRFSEVRYISQRDFNS
                     PDKDSINQSFLTPETFKDPSLSIGDRPYMVLAYMDRTSEAGTNMLNLRLLLADGNEAN
                     QAIQFPGVGNIINILLDNGKFDAAEKLLLKRMMKFQNRLQKQYANSVK"
     gene            4003..4548
                     /locus_tag="PKV31_03200"
     CDS             4003..4548
                     /locus_tag="PKV31_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB91891.1"
                     /translation="MSIIEFNKKIDNISQYLHSFALNLTKNSEDAKDLYQETTYRALV
                     NKDKFAPDTNLKAWLFTIMKNIFINNYRKKVKANTIMDSTANLYYLNAGGSEHNQGET
                     NIFLKELSDMIDQLDDSLRVPFILHYEGFKYQEIADELELPLGTIKSRIFFARKELKK
                     LIKDRYSNIFDIRLKDKKKAV"
     gene            complement(4994..7549)
                     /locus_tag="PKV31_03205"
     CDS             complement(4994..7549)
                     /locus_tag="PKV31_03205"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNB91892.1"
                     /translation="MGKTVIKKEKTTYTQDEAIEASLKYFGGDDLAARVWVNKYALKD
                     SHGNLYEKTPDDMHRRIASEIARVEKNYPNPLSEEEVFELIRNFKYIIPQGSPMTGIG
                     NPYQIASLSNCFVIGHDGPSDSYGGIMKIDQEQVQLMKRRGGVGHDLSHIRPKGSPVK
                     NSALTSTGIVPFMERFSNSTREVAQDGRRGALMLSVSIKHPDSESFIDAKLEEGKVTG
                     ANVSVRIDDEFMRAVQDQSAYQQQYPLMSTDPMYVKKIDAAALWKKIVHNAWRSAEPG
                     ILFWDTIIRESVPDCYADLGYKTVSTNPCGEIPLCPYDSCRLLAINLFSYVENPFTGE
                     ARFNWDLFKQHIHFAQRIMDDIIDLELEKIDEILRKVDEDPESDQIKSIERNLWLSIR
                     EKAHQGRRTGIGITAEGDMLAALGYRYGSPEGIDFSVEVHKTVALEAYRASVHAAKER
                     GAFGIYDAEREKNNPFIQRIKEADAQLYYEMLEYGRRNIAMLTIAPTGTTSLMSQTTS
                     GIEPVFLPVYKRRRKVNPNDKNVRVDFVDEVGDSWEEYAVFHHRFRQWMEVNGHDTKK
                     HYTHEELDNLVKASPYYKATSNDVDYLNKVRMQGAIQKWVDHSISVTINMPNDVSEET
                     VGQCYMEAWKAGCKGVTVYRDGSRSGVLVAAKDNKNAEAAADEPAPFPTTRPQVLDAE
                     IVRFQNNREKWIAFIGLIDGRPFEIFTGLADDEDGILIPRWVNQGLIIKNKEADGTSR
                     YDFQFINGRGYKTTIEGLSYKFNPEYWNYAKLISGALRYSMPIEKVVDLINSLQLDES
                     INTWKNGVARALKRYIPDGTEARKAKCSNCSSTNLHYQEGCLTCKDCGNSKCG"
     gene            7878..8990
                     /gene="mutY"
                     /locus_tag="PKV31_03210"
     CDS             7878..8990
                     /gene="mutY"
                     /locus_tag="PKV31_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNB91893.1"
                     /translation="MHNFSLSKNSYIDFRNKINAWYHPDFRKMPWKGEKDPYKIWISE
                     VILQQTRVQQGWEYYVNFLEKFPTLQVLASSDIDDVLKTWQGLGYYSRARNLHLGARR
                     VVEHFGGRLPANYHDLLKIPGIGPYTAAAIASFAFGLPYAVVDGNVVRVLARYFNIEA
                     AFDSTAGRKIFSIIAGNVLEPADPGRFNQAMMDLGATICTPTATRCGICPLRDGCMAF
                     KLDRIKQLPIKPKKIIRRERFFNYLIIRSTDSCLLFRRQKGDIYSGMYEFLLWENEKE
                     ASTMDMGQWLQDIGINGRAEPILKLPPQILTHQKIIATFYIIHVADLPTLESAIEVAF
                     NDLPQFAFPKFIQTAIDRIDLKKIEGVIQPQISHNS"
     gene            9436..9972
                     /locus_tag="PKV31_03215"
     CDS             9436..9972
                     /locus_tag="PKV31_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB91894.1"
                     /translation="MKKILLLCTFSFLTISISFSQKGSSFIIANIGVDNYKIVNNEYT
                     KTFKVGGGYGYSITEPMALGAEFSYSTFKIKNALTTSSSTSWSAGPFIRSTFPLSDMI
                     SFYSQLGAGYQTEGNKTASGFYAYLNPAAMLKVDKVTYITFTTGLIRYDNIKSFYSRF
                     RFLYGNPTLGVMFKINGK"
     gene            complement(10043..11377)
                     /locus_tag="PKV31_03220"
     CDS             complement(10043..11377)
                     /locus_tag="PKV31_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB91895.1"
                     /translation="MKVKLFVLIGLITSIQYSQGQLPSIPATLSLEEARQLAIKNNPT
                     ILNSNLNIAISQKQVEEARWKKIPQVYTNYDIRSNLIIPTTPVPARAFDPAASENVLL
                     PLKFASRWSSNIGLNAHYDLFNPQTRGAVREAEHQTLISTVEAAISASDLQFAINLDY
                     VACIIARKQVVMAALDTAIMTRILSVTSDRYKAGRSTDADINNAMANKNRSVSDYFRA
                     ENILAQAGARLLTDLGLDPAQSEMPILTDSIPVLMTLFVKDVHNAPEPLHLQRIAHQQ
                     TLSEIRLQNTRDGFLPTVSLNGFLGANYYDNGFNIFNESHWYGNSYLGLSVTIPITQG
                     LDRIKKADVLKLQILADRNRYLTEQNQHNFEVSQARNDYLFRKRDLDLMEQSMELASK
                     NFKLSLDQFKEGRVMVSELSTSELHYRQAKAEYLQAAYDLIVAGMRLEKAIR"
     gene            complement(11393..>11838)
                     /locus_tag="PKV31_03225"
     CDS             complement(11393..>11838)
                     /locus_tag="PKV31_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB91896.1"
                     /translation="GVKAWELQDLRQSTVRELLSSTNMGVSFGTLVVFAIITGFFIIG
                     LTLYSAVLDRLRDYGTYKAIGATNGYVRRLILAQAILFALTGYVIALTLLFFFKQGMA
                     KAGLIVQITPSLAGFLLFATLFISVGGSLFAISKINKLEPASVFK"
BASE COUNT         3267 a         2507 c         2548 g         3516 t
ORIGIN      
        1 agctgcactg aagaaatacg cagttgcaag gcatccctca ttcggtaaga tttatgcatt
       61 cgaggttaat gcctacggat cggccaatct gatggacgat gccaatgtgc caagtctgct
      121 gtcattaccc tatttggaca ctgtcagcct caaggacaag aactatcgta ataccaggaa
      181 atacatcctg tccaatgaaa acccattttt cttcaagggt aaggccggag aaggtattgg
      241 cggtccgcat accgggatgg atatgatatg gccgctgggc atcactatga gaggactcac
      301 gagctccgat gatcatgaga tacgattttg tatccgtcag ttgctggcaa cacatggtgg
      361 gacaggtttc atgcacgaat cgtttcacaa ggatgatgct acaaatttta cccgaaaatg
      421 gtttgcgtgg tccaataccc tgttcggaga attcctgtgg aagatttatc gggagaagcc
      481 ggcactgttg gatgtataag aagagagtag gcgcctacat tggcactaat tttatttgaa
      541 ttgttgtact gatctttcat gccggggaaa gttcatcctg atggaatttt ttcaaagatt
      601 gattaatagc ctcctcgcat cggcctcgcc cgacaatatg gttttggggg gagttgcaat
      661 attatcaaag aagggatgtt tttcacggta cccgggttgg acggattttg aaaacatact
      721 atcgaacccc atcgtagtaa agaaattctc atcgcactga catatgtaac atgccgcata
      781 tctcttctca gaagttggca tttattccag taactcttaa tgcaccgggt ttttgattcg
      841 cgcctgcaaa taataccgaa ggtacagcat agctatccgg acaagtatac caaaaattta
      901 tggactatta tataaatcat cttggtataa ctttcagatc ctgttgtctt tgatttcctc
      961 tacgacctca ggattaagca aagtactggt atctcccaga ttatttaagt cattttctgc
     1021 tattttccgg agaatacggc gcatgatttt accgcttctg gtcttaggca gccctgatac
     1081 aaactgaatt ttgtccaatt tggcgattgg cccgatctgt tcagctatga gggcatttat
     1141 ttcagccgtc aaaatgtcat atttcttacc ttttgcttca tcggccagaa tgacaaagcc
     1201 atatagtgca ttgcccttga tgtcatgtgg gaagcctaca atagcacttt cggcgacatc
     1261 atcgtgaagg ttgatgacgt cctcaatcgg ggctgtgcct aagttgtggc ctgaaacgat
     1321 gatgatgtca tcgactcttc cggtaattct gtagtaaccg gtctcgtccc ttagagcacc
     1381 gtcaccggtg aagtattttc cgggataagt cgaaaaataa gtatccttat atcgttgatg
     1441 gtctccatag attgttctgg ccatccccgg ccatgggtat tttatacaaa gatttccgcc
     1501 ggattgattt ccatggatct cattgctaag ttcatccata aggacaggct ggattcccgg
     1561 aagagggagt gtagcatgac ttggcttagt gggggtaacg aaggccaggg gactgatcag
     1621 gatgccgccg gtttctgtct gccaccaggt gtccacaaga gggcaacgtc tgtgcccgat
     1681 gttttcattg taccagtgcc aggcttcttc gttgatgggt tctccgaccg atccaatcac
     1741 cttcaggctg gataagtctt gacgctgtac aaagtcaaga ctttccttgg ccagtgatcg
     1801 gatagcagta ggtgccgtat aaaaaatatt gaccttgtgt ttttcaatca cttgccagaa
     1861 tcttccgtag tccgggtagg atgggattcc ctcaaagatc aatgttgtcg caccgttgag
     1921 caaaggacca tatacgatgt agctatgccc tgtcacccaa ccgatatcgg ctgtacacca
     1981 atacacgtct ttcttcttat aaccgaagac attcttgaag gtatagccca tgaagaccat
     2041 ataaccggct gttgtatgca gcataccctt gggcttgccc gtacttcctg aggtataaag
     2101 gataaatagc gggtcttcag cgtccaccac ctcagctaca tgtagagcag cagcctcatc
     2161 cagcaatggt tgcaaccact gatcacgtcc ttttttcatg acgacatcac ctccggtccg
     2221 cctgaccacc aggacacgtt ctactccggg gcattgatgc agagcctcat ctacgatatg
     2281 ttttaggtcg atggttttat taccccgaaa agatccatcg gaggtgatga cagttttgca
     2341 atcacaatca ttgatcctag ctgcaagagc cgatgcagaa aatcccgcaa aaacaacaga
     2401 atgaaccgcc ccaatccggg cacaagccag caacgaaaca gccagttcgg gaatcatagg
     2461 taggtaaatg cacacccgat ctccctttct gataccctga tcggtcaata cattcgccat
     2521 ttttgcaacg cgtgcataca attccctata ggtgatgtgt tgcgccggct cattgacatc
     2581 attgggttcg aagatgattg ccgttttatc gccttgcgtc tgcaggtgcc tgtcgatgca
     2641 gttctgagtg atgttgagct ttgcattcct aaaccattca aagcgagctt ccttcatatc
     2701 aaaatcaagt accttattcc aacggctgtg ccagtcaaaa ttttccgctg caattttttc
     2761 ccagaatccg gttggatctt taattgattt ccggtattcc ttgaagtatt tctcgaggtt
     2821 ttttatttta tattttccca tgatccatgt gcatttgaat cataaaatta aggaataatc
     2881 cactataatc aaaagggatc cttttttgac agataaaaac agaccagtga ttcgaaatta
     2941 atgaccgagt tttactttac tgaattggca tattgttttt gcaacctgtt ctggaatttc
     3001 atcatacgct tgagcagtaa tttctcggca gcatcgaact tgccattatc aagcaggatg
     3061 ttgatgatat taccaactcc gggaaactgg atggcttggt ttgcctcatt gccgtctgcc
     3121 aataaaagcc tgagattcag catattggtt cctgcttcag acgtgcggtc catataggca
     3181 agcaccatat atggccggtc accgatggaa agcgaggggt ctttgaacgt ttcaggagtt
     3241 agaaaggatt gattgatact atccttatca gggctgttga aatccctttg agagatatac
     3301 cgcacttccg aaaatctata tacactgtca atagtgtgtc taagaaccct gattaagccg
     3361 tcccttctgg tagttagcgc gtgcaattgc gcttgtgctc tttcgcgttc cttttttccc
     3421 gataagctgt cgccaatgat tgcgttataa tatttttctt gcttatcaaa ggatggtagc
     3481 cttacaatga gaaagccttt cttcagcagt gctgcctgcc ctgtggctga agaaggggac
     3541 tcgctctggg ccggcaatgt gtcaataatg catatcaacc aacatatcat tataaaaagg
     3601 cgaataccga tcataaaaga ttttgaataa gaggtaaaaa taagtgaatc cgggaagtaa
     3661 tttcggtaac agaaacatta agttaacaaa ttatggaaac taatgagtag attagaatcg
     3721 ttctaaatat gatcctgtaa aaagtggcag gggacactgt caaaatggct accatggtaa
     3781 tatggagcca ttattgcagt atttaggaca aactttcaat tatttttgaa actttaacaa
     3841 aacattaatt gtggaataat atactgatta tcaaaaagat gatggtttat tttttcggat
     3901 ttatatggtt aagtgacagg atattgagag gtttgtttaa cgcatagtgc attttcgtta
     3961 aacaaagtct tatatttacg gaagcaaact aaagtacaag ctatgtcaat tatagaattt
     4021 aataaaaaga tagacaacat atctcagtat ctgcattcct ttgcattgaa tctgaccaag
     4081 aattcggagg atgcaaagga cttgtatcag gaaacaacct accgagccct cgtcaataaa
     4141 gataaatttg caccggatac caacctaaag gcatggttat ttaccattat gaaaaatatc
     4201 tttatcaaca attaccgtaa gaaagtgaag gccaacacca tcatggactc tactgccaat
     4261 ttgtattatc tcaatgcggg cggttctgaa cacaatcaag gtgagaccaa tatctttctg
     4321 aaagaattga gtgatatgat agatcagttg gatgattcac tcagggtgcc tttcatttta
     4381 cattatgaag gatttaaata tcaggagatt gcggatgagt tggagttgcc gctgggtacc
     4441 atcaagagtc gtatattctt tgcgcgcaag gagttgaaga aattgatcaa ggatcgatat
     4501 agtaatattt ttgatatcag attgaaagat aaaaagaaag ccgtataggc ataatgggtt
     4561 tattacaaaa gcagagaaca aggcattgta atttgttctc tgcttttttt tacacccaag
     4621 tgtactttat tttaccatac tcatggtgaa taacaatggg ttgagccggt tgtgataagg
     4681 gctggcgaca atcaaatcct tcggcatgaa gtaggtagga aaataataat ctaaatcata
     4741 agaggggaag gagtcttgtt gataatggaa ataaattgat gcctaaatat gctggaaata
     4801 tttgggcaat tagaccagaa tgtccggaaa taaaggtaag taggttatta cttatctacc
     4861 taattcgctt agccccctaa acttcacttc tacttagata caattgattt tgtctgcttg
     4921 tcgcataacc tggcaatatc aaataaaaaa gccgatgctg aagacatcgg ctttttcgta
     4981 atatatttgg atattatcca catttgctgt taccgcaatc tttacaggtc aggcatcctt
     5041 cctggtaatg cagattggtg gatgagcagt tagagcattt agccttgcgc gcttccgtgc
     5101 catccggtat atatcgcttg agcgcacggg ccacaccatt cttccaggta ttgatggatt
     5161 cgtctaactg aaggctattg atcagatcca ccactttttc gatcggcatg gagtaacgca
     5221 atgctccgga tatcaacttg gcatagttcc agtactcagg gttgaattta taagataatc
     5281 cctcaattgt ggtcttgtaa ccgcgaccat tgataaactg gaagtcgtag cgagaagtgc
     5341 cgtcagcctc tttgtttttg atgatcaggc cttgattgac ccacctggga atcaggattc
     5401 cgtcctcatc gtcggccaat ccggtgaata tttcaaaggg gcgtccatct atcaaaccta
     5461 tgaaggcgat ccatttctcc cgattatttt gaaatctgac aatttctgca tccagtacct
     5521 gtgggcgtgt tgtagggaat ggtgctggtt cgtcagcggc tgcttcagca tttttattat
     5581 cttttgcggc aaccaggaca ccagaccttg agccgtcacg atagacagtt actcctttac
     5641 aacctgcctt ccaagcctcc atataacact ggccgactgt ttcctcgctt acatcattgg
     5701 gcatattgat ggtcaccgag atggaatgat ccacccattt ttggattgca ccctgcattc
     5761 ttaccttatt taggtagtca acatcattag aggttgcctt gtagtaagga gatgccttta
     5821 cgagattatc caattcttca tgagtataat gtttttttgt gtcgtggcca ttgacctcca
     5881 tccactgtcg gaatctgtga tggaatactg catattcttc ccaggagtcg cctacctcat
     5941 caacaaaatc cactctgaca ttcttgtcat tggggttgac cttgcggcgg cgtttgtaca
     6001 caggaaggaa tacaggttcg atacctgatg ttgtctgaga cattaagctg gtggttccgg
     6061 taggggcaat ggtaagcatg gcgatatttc tccttccata ctcaagcatc tcgtagtaca
     6121 attgggcatc cgcttctttg attcgctgga taaacggatt gttcttttct cgttcggcgt
     6181 catagatacc gaatgcaccc ctttcctttg ctgcgtgaac ggacgcgcga taggcttcaa
     6241 gggccacagt tttatgcact tcaactgaaa aatcgatgcc ttcaggactt ccgtaacgat
     6301 agcccaaagc cgccagcatg tcaccctctg ctgtaatgcc gattcctgta cgtcttccct
     6361 ggtgtgcttt ttcgcggatg gataaccaaa gattcctttc gatgctcttg atctggtcgc
     6421 tttccggatc ttcgtctact tttctcagta tctcatcaat cttttccagc tcgaggtcga
     6481 tgatatcgtc catgattctc tgtgcgaaat ggatatgttg tttgaataag tcccaattga
     6541 atcttgcctc accggtaaaa ggattttcaa catatgaaaa cagattgatg gccagtagtc
     6601 gacaggaatc gtaggggcaa agcggaatct cgccacaagg attggtcgaa accgtcttgt
     6661 atcccagatc ggcatagcaa tcaggtacgg attcccggat gatagtgtcc cagaaaagga
     6721 taccgggctc ggcagagcgc catgcattgt gcacgatttt cttccaaaga gccgctgcgt
     6781 caatcttctt gacgtacata gggtctgtac tcatgagcgg gtactgctgc tgataggccg
     6841 attgatcctg caccgctcgc atgaattcat cgtcaattct cactgataca ttggctcccg
     6901 tcactttgcc ctcttccagc ttggcatcaa taaaactttc agagtcaggg tgcttgatgc
     6961 tgactgacaa catcaatgcg cctctgcggc catcctgtgc gacttcccgt gtagaattgg
     7021 aaaaccgctc cataaagggc acgatacctg tggaggtcaa tgcggaattc ttgaccggag
     7081 accctttagg tctaatatgt gacaggtcgt gacctacacc gcctcttcgt ttcatcaact
     7141 gcacctgttc ctgatcgatc ttcatgattc cgccataaga gtcagatggg ccatcatggc
     7201 caattacaaa gcaattggac aatgaagcaa tctgatacgg gttgcctatt cctgtcatcg
     7261 gacttccctg agggatgata tatttgaaat ttctgatcag ttcaaaaact tcttcctctg
     7321 aaagggggtt gggataattc ttttctacac gtgcaatttc ggaagcgatc ctgcgatgca
     7381 tatcgtccgg tgtcttttca taaagattgc catgagaatc cttcaacgcg tatttattga
     7441 cccaaaccct cgcagcaagg tcatctccgc caaagtattt taggctggcc tcaatagcct
     7501 catcttgtgt atacgtagtc ttctcttttt tgataactgt ctttcccatt tgtcatttgt
     7561 tttatcatcg aaaggtttta ataaatttta aagaagcaaa tgtatgtaat ttaattataa
     7621 tacaaaattt ttaaggaaaa aataaatatg tttgcataaa ataaattata agtcattgaa
     7681 agacaaatgc aaataaaatt taggatcgaa aaaagtttac aattttgtgc attaatattt
     7741 caaaattaga tacttaacat tccaaaaata gtcaagtatc ataatagata tttaactaat
     7801 gtgttcaaaa tcagacaact tcttttccca aaaaaaattt ccggttttta aaaatttact
     7861 gtttactatt tttgctcatg cacaattttt cactcagtaa gaattcttat atcgatttta
     7921 ggaataaaat caatgcctgg tatcatcccg attttagaaa aatgccctgg aagggagaaa
     7981 aggatcctta caagatatgg atttcagagg ttatcctcca gcaaacacgt gtacaacaag
     8041 gttgggagta ttatgtgaat tttctggaga agtttcccac cttacaggta ttggcttctt
     8101 ctgatataga cgatgtgctg aaaacatggc agggcttggg ttattacagt cgtgcccgta
     8161 atctccatct gggggcgcgc cgggttgtag agcattttgg tggaaggcta ccggccaatt
     8221 accatgattt attgaagatc cccggcatcg gtccttatac agccgccgcc attgcttcat
     8281 ttgctttcgg actcccctat gcggttgtgg acggcaatgt ggttagggtt cttgcgcgat
     8341 atttcaatat cgaggctgca tttgattcaa cagcaggaag gaagattttt tcaatcattg
     8401 caggaaatgt cctggaacct gctgatcccg gtcgattcaa tcaggcaatg atggacctcg
     8461 gagccacaat atgtaccccg acagcaacac gatgtggcat ttgtcccctc agagatggtt
     8521 gcatggcatt taaattggac aggataaaac agctaccaat caaaccgaaa aagataatcc
     8581 gccgggaaag atttttcaat tacctcataa ttcgttccac agactcatgc ttgttgtttc
     8641 gtcgccaaaa aggagacata tattcaggta tgtatgaatt tcttttgtgg gaaaatgaga
     8701 aggaggcctc gacaatggat atggggcaat ggcttcagga cataggaata aatggccggg
     8761 cggaacctat actcaagctt cctcctcaaa tcctgactca tcagaagata attgccacct
     8821 tctatattat acatgtagct gatttgccca cgttggagtc agccattgag gttgctttca
     8881 atgaccttcc tcaatttgcc tttccaaaat ttatccagac tgccattgat cggatagatt
     8941 tgaagaaaat tgagggagtg attcagcctc aaatatctca caatagttga tattttgata
     9001 actgatcatt agatagcaag gatagaccat tccgacacaa tgagaccttc aatatcctgt
     9061 tagcccatcc agttgcatat taaccaaagt ttcaatttta caaaacaagg atttatctat
     9121 agtttgactg aaattgctat agatggaatg tgttgttcga tcctctgtgt ccatcgggaa
     9181 atattctatt tagagcatta catgttggca tattgattcg ccggatgagc aaagacaaag
     9241 atgaaggcga tgtgaaataa ctcctcctat gtattttata atagatactt tagggataaa
     9301 cggggtcttc tatacttatt tagagaccgg ttcttgtgat gttaactaag cgtttctgtc
     9361 aaaaaattca aatatttgac tgaatgtttg gttacaatgc aatatattta tcatttatta
     9421 aaaaaattaa gtattatgaa aaagattctc ttgttgtgta cattttcttt tctaaccatc
     9481 tccatatctt ttagccaaaa aggttcatcc ttcatcattg ccaatatcgg tgtagataat
     9541 tacaagattg taaacaatga atacactaaa accttcaaag ttggtggtgg atatggctat
     9601 agtattactg agccgatggc attgggtgca gagttcagct attcaacctt taaaataaaa
     9661 aacgcactca ctacgtcctc atcaaccagt tggtcagcag gaccctttat ccgttcaact
     9721 tttccactca gtgacatgat ttctttttat tctcaattgg gtgcagggta tcagacggaa
     9781 gggaataaga ccgcatcagg attttatgcc tatctgaatc cggctgccat gctgaaggtg
     9841 gacaaagtga catacattac cttcacaacc ggcctcatca gatacgataa tattaaatca
     9901 ttttatagtc gtttccgttt tctatatggg aatccaactc tcggcgtgat gtttaaaatc
     9961 aacgggaaat aaatattcga aggcaaattt tggttcacag aattgtattt tgggtaagta
    10021 aagggggcat taaaagcacg gcttaccgta ttgccttttc aagcctcatt ccggctacta
    10081 tcaggtcata ggctgcctga aggtattcgg cttttgcttg tctataatgc aactcactgg
    10141 tcgaaagttc ggaaaccatt acacgaccct ccttaaattg atctaaggat aacttgaaat
    10201 tcttgcttgc cagttccatt gattgttcca tgagatcaag atctcttttc cgaaacaaat
    10261 agtcattgcg ggcttgtgat acttcaaagt tgtgttggtt ctgctccgtg agatatctat
    10321 tccggtcggc caatatctgc aacttcagta catcagcctt ttttatacga tccaatcctt
    10381 gtgtgatagg tatcgtgacg gaaaggccca gatagctgtt gccataccaa tgactttcat
    10441 tgaagatatt aaagccattg tcataataat tggcgcccaa aaaaccattg agtgaaaccg
    10501 ttggcagaaa gccatccctt gtattttgaa gtcggatctc agagagagtc tgctgatgag
    10561 cgatcctctg cagatggagt ggctcgggag cattgtggac gtctttgaca aataatgtca
    10621 ttaataccgg aatactatca gtaagtattg gcatttcaga ttgcgcaggg tctaatccga
    10681 gatctgtaag caacctcgct cctgcctgtg ccaatatatt ttcggccctg aaatagtcag
    10741 acactgacct attcttattt gccatggcgt tgtttatatc ggcatccgta gatctacccg
    10801 ctttgtatct gtcacttgtc acggagagta tgcgggtcat aatagccgtg tcgagggcag
    10861 ccattaccac ttgcttacgg gcgatgatac atgccacata gtcgagattt atcgcaaact
    10921 gtagatctga tgccgatatg gcggcctcta ccgtactgat aagagtttga tgctcggctt
    10981 cacgtacagc accgcgtgtc tgtggattga acaggtcata atgggcattg aggccaatgt
    11041 tgctgctcca tcggctggca aacttcaagg gtaaaagcac attctccgaa gctgccggat
    11101 caaaggcgcg cgccggaaca ggagttgtcg gaataattag gttgctgcgg atatcgtaat
    11161 tggtatatac ttgcgggatt ttcttccatc gggcttcctc gacttgtttt tggcttatgg
    11221 cgatattaag attgctgttg aggatggttg gattgttttt aatagcgagt tgtcgtgctt
    11281 cttccaacga aagggttgcc ggtatggatg gtagttgtcc ctggctatat tgtattgagg
    11341 taatcaggcc aatcaggacg aataacttca ctttcatttt tagtacaatt tgttatttga
    11401 aaactgaagc aggctccagt ttattgattt ttgaaatggc aaacagtgag ccgcccacgg
    11461 aaataaacaa ggtagcaaac aagagaaatc ctgccagtga aggcgtaatc tgtacaatta
    11521 atccggcttt ggccatgcct tgcttgaaaa agaatagcaa tgtcaaggcg ataacatagc
    11581 cggtaagcgc aaataaaatc gcctgtgcaa ggatcaatcg ccgtacataa ccattcgtcg
    11641 caccaatggc cttataggtt ccgtaatccc gcaatcgatc caacacggcg gagtacaatg
    11701 tcaggcctat gataaagaaa cctgtgatga tggcaaaaac cacaagtgtt ccgaagctaa
    11761 cacccatatt cgtggaactg agcaattcgc gtactgtcga ttggcgcaaa tcctgcaatt
    11821 cccaagcctt gacaccat
//
LOCUS       DAOQMC010000046        11775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2096_length_11775_cov_5.386860, whole genome shotgun sequence.
ACCESSION   DAOQMC010000046 DAOQMC010000000
VERSION     DAOQMC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_11775_cov_5.386860"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1294
                     /locus_tag="PKV31_03230"
     CDS             77..1294
                     /locus_tag="PKV31_03230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNB91897.1"
                     /translation="MKVDYIALSIPIFFILIAIELAYTYSKKLRYFRLNDSLANLSQG
                     IGQQIIGVFMKTALFFCYLYIYEHWRFFTLPNTIWIWILLFFIVDFFYYWFHRKSHEI
                     NALWAAHVVHHQSEEYNLTVALRQSWFQGWFSWIFYLPVAFIGFNPIMFLTMTAFNTL
                     YQFWIHTRTIGNMGWLEYIFNTPSHHRVHHGSNPKYIDKNHGGTLIIWDRLFGTFQKE
                     EETVYYGITKPVNSWNPVWANFQNWADIVKLAGMMRTWKDKILVFINPPGWQPDYLGG
                     PLYPEEIDIENYSKYDKKSNVHLTAYSFFLFLIALAVGTALLFRFPQLNMLQCYATAA
                     FVMATLVSCGALFEQNFWLKAFEFSRILLSFLLVPLYLESSFSIIRWIFVAVQFISLA
                     WFISISKSIKSEV"
     gene            1284..1961
                     /locus_tag="PKV31_03235"
     CDS             1284..1961
                     /locus_tag="PKV31_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNB91898.1"
                     /translation="MKSEKFNLIFFLLTIVHLFAIEAEFGWLEVSTKPLLVAALMYWF
                     YINTALKNRFAQKVFAALIWSWMGDVLLMMVPINANFFLFGLGAFLIAQLAYASAFYE
                     DARLKGDRPVWLMPFAIAFFTTYCMLLFGYMAPKLGGLKIPVLLYAIVISIMAIMAWT
                     RYGTAKGRSYWLVALGAGLFVISDSVLAINKFSNPIPNAGIIIMLTYILAQYGITMGA
                     IARIRDR"
     gene            complement(2140..2364)
                     /locus_tag="PKV31_03240"
     CDS             complement(2140..2364)
                     /locus_tag="PKV31_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91899.1"
                     /translation="MEDKNMKFKTNINCSGCIEKVTPFLDLSAGAGNWEVDTTNKDKI
                     LTVQGLDKSEAEIMDSVKRAGYRIEVLEAP"
     gene            complement(2486..3160)
                     /locus_tag="PKV31_03245"
     CDS             complement(2486..3160)
                     /locus_tag="PKV31_03245"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB91900.1"
                     /translation="MKERIEISVGRDVSNDIVLNDPSVSLFHCTVSNETGGSVTISDL
                     NSANGTWVNNKRVVRKNITLDDKIGIGNFEPDPADFFKKIIELTDKRRKDFSEEFQEI
                     LRVFTIYQRKKDKITDTPKTGLILRLVFGVGLILILVFFKDLIPDFQTRYIIIMSIGL
                     LSFVSALFSAPQSKKNKMLDELRLEYEDKLVCPKCKSRLINHSLTYWQGKQQCPQPGC
                     DAVYHK"
     gene            complement(3157..3849)
                     /locus_tag="PKV31_03250"
     CDS             complement(3157..3849)
                     /locus_tag="PKV31_03250"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNB91901.1"
                     /translation="MDTIIFFNTKPEIKVAVEELVFSSQRPYTLYFPATYEQLGHLLV
                     KNTDALVFYSITAFTPDENMRLRYLLMNFNTVRFCLIGTVDLANLAWKLNVFHFEESP
                     VRYERLIFVYKKYVHQKTPLIDELIVKHNEEMLRIDYKRITHLSASGNYTLIHLNDGK
                     AILQTRQLGMFDFLTEKDLNFVRIHRSLMVNLRNISKIGNGKITFFGKENEIDISASL
                     ESKLKRTLLGRV"
     gene            complement(3909..4352)
                     /locus_tag="PKV31_03255"
     CDS             complement(3909..4352)
                     /locus_tag="PKV31_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91902.1"
                     /translation="MKYLHVMLLLMILGLLACQKNKPSQEDIIAAEAEMDTIRKIITE
                     DSTISKAIIKDKKVEAKHKEDAERINKEIEKSPLKDLPCEKIIAEFRISVDKYCTGKI
                     TQEEMIASIPDLQDTKVKVCINNLFQEELDKINAKLEKCLESKGD"
     gene            complement(4356..5837)
                     /locus_tag="PKV31_03260"
     CDS             complement(4356..5837)
                     /locus_tag="PKV31_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB91903.1"
                     /translation="MKEIRSIFIKNEIKKLFQHGRSHFILLGIVFSLLIIALNQSIFG
                     LKALNEKYNNPFANYIQVATPKNTDLDRETLAKTLIQKFSAEPLKGKLGISRINELSY
                     EFMPFCNPAIDKVKTYKGRTYSKDDPLYQELVKASGDNIITTNKIEEDDITGQYGVII
                     TENMVLDLGYDPRSIKTVPLKLGGKEFVLVPVIVVVKYLPQFSDFMITEDFSYINNQS
                     IEQTNFVDLGSSNFYSIASFNDTSKVLEDARKVLKKDFKDVQCKLIDIDQLNKVSVCE
                     FQLNEYLSAEDKEDMTASLNTGNNKDKLYNFYTLHYVNTENAEKFAPDYLEFQFASLD
                     SIKAFQEVCMQNSLDPDMQIIKSRHLFAEVGSISRIVSGGFILFSILMLFIFTNSFIS
                     RHIEEIKPNLGTLLAYGMSGKMIVNNYLIVIFIIYFAAFVIGLVLALFFGLILYFTFS
                     VPIIYNYRVVLLVFIGILITLIYSRSIIFRIFNNTPGDLIYKR"
     gene            complement(5834..6646)
                     /locus_tag="PKV31_03265"
     CDS             complement(5834..6646)
                     /locus_tag="PKV31_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB91904.1"
                     /translation="MRRILFSIKDLKCSYDSGKIVLDIDRLDIYDGQITFIVGKSGSG
                     KSTLLETLGFMNDTFSSRTPEQVVFHDAGSNDKTDLASLWLKPLDMHSAFRNKNYSFI
                     FQENNLLEHFNSSENILIPILLSGKDKSEGMKEIQQAFERIHLPPDIFNRNIRLLSGG
                     QRQRISFLRALLANKPVLFGDEPTGNLDPITAKTLMTMVREDISRSGSSAIIVSHDLN
                     LAHLFADRIVTLKVDENRQCGIIGGIFERKDNGWLDQLSHKNVEHAEITSLL"
     gene            complement(6649..9198)
                     /locus_tag="PKV31_03270"
     CDS             complement(6649..9198)
                     /locus_tag="PKV31_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91905.1"
                     /translation="MIKRYFSIFVFCFLVKGLSFGQAITLMEAPADYSPPLDYYFSGA
                     NNDLNLYINNQNLKPAGDKRSRRTWIIINDREGNIAYNGPSVTSGTKSTLGFLESFAV
                     SEVKGSFAHIFRIDGGVPHGKEIPAGAIAVDMGWVERSTVLQWGMGLVDSKSKISLKA
                     ILLNKAEDIKNLVGLSNKEIVRIYDSPYSKKTLGDRNIYEIFYILKKENGRALLSTEY
                     QFGSSPKTNIIGWVDLSRLSKWNTRIALEPNHNIMACEERKADENKRVSFFANSRDAN
                     QFLKTGDKDNILFSQDPVAISPEFLAKSNPRRYKGSILRYPILANPGNNLFQTGIIGK
                     VTTVGSGQESGSIDPAIYSKIVEAQRKRSEDNKIVNIFFLIEGSTEMNTYKSQLADLI
                     DKLPTNFPGANIVNVGCGIYRDFQSNQVPFTFRKLTQNRKDVAAFIDKEVFYRPLDDD
                     RETVFRFALKKTLEIHGGFQPGQRNILMIIGNAADFSNNTVRKLKTEKKEYLISGNEM
                     GSIYKALADLDMNIIFIQPHSEEGRFSRKFQSDGLELMGDVAGRIFSENEKISQKLKI
                     NLNGPEMPECQEGKLVEATGGVNYFGFYSAPISKELSPAKLMDEIKISLDACNKLRNS
                     HIKANADVIEKGKSLDVSPGEFKAYFMRFIKQELEDIDPASITKLLQEKYQLFIEAYL
                     PKHFNGAKYDSYSYVMFLPKNELYGFIQSLNDVAGKYNQGYSMSELRQSLKDAVTELV
                     LTVSSQNDRSRVGQLDMSSTLEIILGLKEEGVVLFQQYSNKKLADLTKKGVFPDEEVS
                     KLAKGFSDLKLKLEDIYKSGSRYEFCFRTQGDNDNIYFWIALEDIFDKSFF"
     gene            complement(9204..10745)
                     /locus_tag="PKV31_03275"
     CDS             complement(9204..10745)
                     /locus_tag="PKV31_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91906.1"
                     /translation="MKIRRIHIFWMIFGIILFSSNIFGQDCKLEKGDWSVIDRSGIEQ
                     MYKGKIRDALNQFLANYNRYADIYDEKYDWRESNFLKLLDKGNSTTPYLNDMLLHPTE
                     GMAIGYINQIAEYFPNGFKFCITGLTVKDIRALQSDLYFVKIQVVKRTENYVDAGQVV
                     NKERDITLDFDMIVFSMEDSEELTAAISSIELVRVSAAGAGGHDVAMEAPKVKKEPKQ
                     KQEKKEREPMAQPQDNYNSYEGKKKTRDFKMVYLDGGYGMPRNEVSAGIQESDFKSTG
                     FHIGLGGEYWKSFSPSNKVFYSIGLLFRYHNLKDEVVGSDVKLNTFSSHYNNNLSRAP
                     KTSEFRMQAGQISTNVVGVYAPIGLVYNFGRGNDPRVFMVQANIMPGYIFSSTRTVST
                     NSASIYSYYGDDSSIDNCEITAVKIDKNLTLKNRFTLAARLSPTYVYESKSWQGKRGF
                     GFVFGLDFTLGLMPVIVTENPQLIESYDSSNRTFTPISDASFFYQTKIYHSVGGRIGI
                     YYVLN"
     gene            complement(10768..>11775)
                     /locus_tag="PKV31_03280"
     CDS             complement(10768..>11775)
                     /locus_tag="PKV31_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91907.1"
                     /translation="DIPIDSIVNFCTQPTKTEVTFKLNGELGPKAYASATWWLDTDII
                     GNSKEIVIDFNKQTKNFKLKVKTFDQAGCEMSQTISFIQNEPTSDIFDEYKCGVYKLK
                     PGYSLEGSSLPSNIQDLGSGFYFITGKVNLEYTLKNDAGECRSKMDKDFVSKITSGEF
                     ADDQFVIDECGPFLLARHKEGFCYDWYRLDDQNKAIRIDSAGNSWLKVTQENLIKNKY
                     IAIGYDCKNSCDSTFISTRSDGDEVVIPCGGTDSKSLKAYPNPGSGHMYIQAAGLSPG
                     KQTLDIYNMMGTRIYSMTIGQAESNLLTEVSLLDIPNGIYLVRLGNDKAEVTGKIIVI
                     H"
BASE COUNT         3232 a         2588 c         2185 g         3770 t
ORIGIN      
        1 tttaatcatt ctcattcatt ttctcccaac gaaaattttc aatgttttta cctatttttg
       61 aacaaagtta aatatcatga aggttgacta tatagcactt tcaataccca tcttctttat
      121 cctgattgct attgagcttg cctataccta ttccaaaaaa cttcgatatt tcaggctcaa
      181 cgattccctc gctaatctca gtcagggtat tggccagcaa atcatcggtg ttttcatgaa
      241 gactgctttg tttttctgtt atctgtatat ctatgaacat tggcgctttt ttaccttgcc
      301 caataccatc tggatatgga tattgttgtt tttcattgtt gactttttct actattggtt
      361 ccacagaaaa agccatgaga tcaatgcttt gtgggcagca catgtcgtcc atcatcaatc
      421 tgaggaatac aatcttaccg tggcgctgag acaatcctgg tttcagggct ggtttagctg
      481 gatcttctac cttcctgttg cattcatagg gttcaatccc atcatgtttc ttacgatgac
      541 tgccttcaac accctgtatc aattctggat tcacacccgc accatcggca atatgggttg
      601 gctggaatat atattcaaca cgccttcaca tcaccgggta caccacggct ccaacccaaa
      661 gtatattgat aagaatcacg gaggcacact aatcatctgg gaccgactgt ttgggacctt
      721 ccaaaaggaa gaagagacag tatattacgg aattaccaag cctgtcaaca gctggaatcc
      781 tgtatgggcc aattttcaga actgggctga cattgtcaag cttgccggaa tgatgcgcac
      841 ctggaaagat aaaatacttg tcttcatcaa tcctcccggc tggcaacctg attacctggg
      901 cggtcctcta tatccggaag aaatagatat tgaaaattat agcaaatacg ataagaaatc
      961 taatgtacat ctcactgcat atagcttttt tctgttcctg attgccctgg ccgtcggtac
     1021 agccctgctg tttcgttttc cacagctcaa catgctacaa tgctatgcaa ccgcagcctt
     1081 tgtgatggcg acattggtca gctgtggtgc tttatttgag caaaatttct ggctgaaggc
     1141 gtttgaattc agtcggatcc tactttcctt tctattggtg ccattgtatc tggaatcatc
     1201 gttttctatc atcagatgga tctttgttgc ggtgcaattc atatctttag cctggtttat
     1261 ctcaatctca aaatcgatta aaagtgaagt ctgaaaaatt caacctgatt tttttcttac
     1321 tgacaatcgt ccaccttttt gctatagagg cagagttcgg gtggctggaa gtttcgacga
     1381 agcccctttt ggtagctgcg ttgatgtatt ggttctatat caataccgca ttaaaaaacc
     1441 gatttgcaca aaaagtattc gccgctctca tctggtcatg gatgggtgat gtcttgctga
     1501 tgatggtacc gatcaatgct aacttctttc tgttcggtct gggtgctttc ctgatcgccc
     1561 aacttgctta tgcatccgcc ttttatgagg atgcaaggtt aaagggtgac cgaccggtct
     1621 ggttgatgcc atttgccatc gcatttttta ccacctattg catgttactg ttcggttata
     1681 tggccccaaa gcttggaggg ctgaaaattc ctgttctgct ttacgccatc gttatttcca
     1741 tcatggctat catggcatgg acgcgttatg gcaccgcaaa gggacgttcg tactggcttg
     1801 tagctttggg agccgggctt ttcgttatat ccgattccgt tcttgccatc aacaagtttt
     1861 ccaacccgat acccaatgca ggcatcatca tcatgctcac ctatatcctg gcacaatatg
     1921 gcataacgat gggggcaatt gcccggatca gggacaggta atgcgaatag tgttcaacgg
     1981 tggatttcga tgtaatcctc cgggaaactg gtccgggtcg tacgcttccc ggaatttata
     2041 ttataaatca aactgaaggt acccgattgt gtactttagg cattatacca aaaatttatg
     2101 gactattata taaattatct tggaataaaa tcgcctgtct tatggcgctt ccagcacttc
     2161 gatcctgtag ccggctcttt tcacgctatc cattatttca gcttcagact tgtcgagacc
     2221 ttgcacagta aggatcttgt ccttattcgt tgtatcgact tcccaattgc ctgctccggc
     2281 agatagatca agaaaaggtg tcactttctc tatacatccg ctacaattga tgttggtctt
     2341 aaacttcata tttttatctt ccatagttat ttctcattaa ggtcatgcat gtatgataaa
     2401 cactaaaatt ccgggtttgt ttttgcccca aaatatttga aagggagtga ttgcagccgg
     2461 taaacgatgt ggttaaaata gttgtttatt tgtgatagac ggcatcacat ccgggttgtg
     2521 ggcattgttg cttaccctgc caataagtca ggctatgatt gatcagcctg gatttgcatt
     2581 taggacacac caatttatct tcatattcca gtcttaattc gtccagcatt ttattctttt
     2641 tgctttgggg ggcactgaat aatgcagaga cgaaggacag taatccgatc gacataatga
     2701 taatataccg tgtctgaaag tcaggaatca aatccttaaa gaagaccaga atcaaaatca
     2761 aaccgacccc aaatacaagg cgcagtatga gaccggtctt gggagtatca gtgattttat
     2821 ctttttttcg ctgatagatg gtgaatactc gcaaaatttc ctgaaattct tcgctaaagt
     2881 cttttcttct tttatctgtc agctcaataa tttttttgaa aaaatctgcc ggatccggtt
     2941 caaagttgcc tatgccaatc ttatcatcca aggttatatt ttttctcacg acccttttgt
     3001 tgttgaccca tgtaccattg gcagaattga ggtcagagat ggtgaccgat cctccggttt
     3061 cattgcttac agtacagtga aagaggctta cacttggatc attcagcaca atatcattgg
     3121 atacatctct accaaccgat atttctattc gttccttcat actcttccca aaagcgttct
     3181 tttcaactta ctctccagac tcgccgaaat atcaatctca ttttccttcc caaaaaaagt
     3241 aatctttcca tttcctatct ttgaaatatt cctgagattg accatgagcg agcggtgaat
     3301 gcgtacgaag ttcaaatcct tttccgtcag aaaatcaaac atacccaact ggcgcgtctg
     3361 gaggatagct tttccatcat tcaaatgaat aagcgtatag tttccggacg cactaagatg
     3421 ggttatccgt ttatagtcta tacgcaacat ttcttcattg tgtttcacaa tcaattcatc
     3481 tataaggggt gttttttgat gaacatattt cttatagacg aagatcagcc tttcatacct
     3541 tacagggctt tcctcaaagt gaaatacatt caatttccag gcaagattgg ccaggtccac
     3601 ggtaccaatc aaacaaaacc taactgtatt gaaattcatg agcaggtatc gcaggcgcat
     3661 attctcatct ggagtaaacg ctgtgattga ataaaaaacc aatgcatccg tattttttac
     3721 aagcagatgg cccaactgct cataagtagc aggaaaatac aaggtataag ggcgctgcga
     3781 ggaaaataca agttcctcca cagcaacttt tatttccggt ttcgtgttaa aaaatataat
     3841 ggtatccatt gacaaatact atatcagcaa ttactggccg gtgtcatgtt taatatccac
     3901 aatcttactt aatcaccttt ggactcaagg catttctcta gtttggcatt aattttatcc
     3961 aattcctcct gaaagaggtt attgatacag acctttactt tggtgtcctg taagtcaggt
     4021 atactcgcta tcatctcttc ctgtgtgatc tttccggtgc aatatttatc tacactgata
     4081 cgaaattctg cgattatctt ttcgcaaggc aaatctttaa ggggagattt ctcaatttct
     4141 ttattgatgc gttctgcatc ctctttgtgt tttgcctcta cttttttgtc cttaatgata
     4201 gctttggaaa tggtactatc ttcagtaata atcttccgga tggtatccat ttctgcttcg
     4261 gcagcaataa tatcttcttg cgaaggctta tttttttgac aggctaaaag gcccaaaatc
     4321 atcaaaagga gcattacatg caggtatttc attttctatc ttttataaat taagtcgccc
     4381 ggtgtattgt tgaatattcg aaaaatgatg cttcttgaat aaataagggt aatcaagatc
     4441 ccgataaaga caagcaatac aacccggtaa ttatatatta ttggaacact aaatgtaaaa
     4501 taaagaatca agccaaaaaa taatgccagg accagtccga tgacaaatgc cgcaaaatat
     4561 atgataaata ttacaatcaa ataattattt actatcattt tgcctgacat cccataagcc
     4621 agcaaggtac caagattggg ctttatctct tcaatatgcc tggaaataaa actattggta
     4681 aagataaaga gcatcaaaat cgaaaagagg atgaaaccac cggaaacaat tcttgaaata
     4741 gagcccacct cagcgaataa atgcctcgat tttataattt gcatatccgg atcaaggctg
     4801 ttttgcatgc acacttcctg aaatgccttg atactatcca ggcttgcaaa ctgaaattca
     4861 agataatccg gtgcaaattt ttcggcattt tccgtattta cgtagtggag cgtataaaag
     4921 ttgtagagtt tgtccttatt atttcctgta ttcagtgagg cagtcatatc ttccttatcc
     4981 tctgccgaca aatattcatt gagctggaat tcacatactg ataccttgtt gagttgatct
     5041 atgtcaatca acttacattg cacatccttg aaatcttttt tcagtacctt tcgggcatcc
     5101 tccaggactt tggaagtatc attgaaggag gctattgaat aaaaattgct actacccaga
     5161 tctacaaaat tggtctgttc aatactttgg ttattgatat aggagaagtc ttcggtaatc
     5221 atgaaatcag aaaactgagg cagatacttg accacaacga tgacagggac aagaacaaat
     5281 tcctttccgc cgagctttaa gggtaccgtt ttgatcgacc gggggtcata accgagatca
     5341 agaaccatat tctcagtaat gattactcca tattgcccgg tgatatcatc ctcttctatt
     5401 ttgttagtcg taatgatatt gtcacctgag gccttcacca attcctgata taagggatca
     5461 tccttggaat aagttctgcc cttataggtc tttactttgt cgatagccgg attacaaaaa
     5521 ggcatgaact cataactaag ttcgttgatg cgtgaaatgc ccagcttccc ctttaaaggc
     5581 tcggctgaga atttttgaat caatgttttg gcgagggttt ctctgtccaa atcggtattc
     5641 ttgggagtgg ctacctgtat ataattggca aatgggttgt tgtatttttc attcaatgct
     5701 ttcaggccaa atattgactg attcaatgct atgatcagga gagaaaacac aatacccaac
     5761 aggataaaat ggcttctgcc atgttgaaag agcttcttta tttcattttt gataaaaata
     5821 cttctgatct ccttcatagc aagctagtaa tttctgcatg ttccacattt ttatgagaca
     5881 actggtccaa ccagccattg tcttttcttt cgaagatgcc gccaataatt ccacattgtc
     5941 tgttttcatc cacctttagt gtcacaatcc tgtcagcaaa taagtgggca agattgaggt
     6001 cgtgagaaac aatgattgca gaacttccgg aacgacttat atcttcgcga accatagtca
     6061 taagggtttt ggcggtgatt ggatcaaggt tacccgtagg ttcatcgcca aagagaacag
     6121 gtttgttggc taagagtgcc cttaagaagg atatcctttg acgctgtccg ccggaaagca
     6181 acctgatatt tcggttgaag atgtccgggg ggagatgaat tctttcaaat gcttgttgaa
     6241 tttctttcat tccctctgac ttgtcctttc ctgacagtaa tatgggaatt aggatattct
     6301 cactggagtt gaaatgttcc agcaagttgt tttcctggaa aataaagcta taatttttat
     6361 ttctgaatgc tgagtgcata tctaaaggct tgagccacaa tgatgcaaga tccgtcttat
     6421 catttgatcc tgcgtcatgg aagactacct gttccggagt gcgggagctg aaagtatcat
     6481 tcataaagcc cagggtctca agtagcgtac tctttcctga cccggatttg ccgacaataa
     6541 aggtaatctg cccatcatat atatccaggc gatcaatatc aagtactatc ttgcctgaat
     6601 cgtagctaca tttcaggtcc tttatggaaa acaatattct tctcatgcct aaaaaaaact
     6661 tttatcgaag atatcctcta aagcgatcca aaaataaata ttgtcattat cgccttgtgt
     6721 tctaaagcag aactcatacc tactgccact cttataaata tcctccagtt taagtttcag
     6781 atcgctgaac cctttggcga gcttacttac ctcttcatcc gggaacactc cctttttggt
     6841 aaggtctgcc aacttcttgt tggaatattg ctgaaacagc acaacacctt cttccttcaa
     6901 gcccagaata atttcaagcg ttgagctcat atcaagttga ccaaccctcg atctgtcatt
     6961 ttgtgagctg acggtcagaa ccaactcagt aacagcatct tttaaggatt gcctgagttc
     7021 ggacatggaa tacccctgat tgtatttacc tgcaacatca ttgaggcttt gaataaatcc
     7081 atacaattca ttcttaggaa gaaacatcac ataagaatag gaatcatact tggctccatt
     7141 aaaatgtttg ggcaaatagg cttcgataaa aagttgatac ttttcctgaa gcaacttagt
     7201 gatggaagca ggatcaatat cttccaattc ctgcttgatg aatctcataa agtatgcctt
     7261 aaactctccg ggacttacat ccagactctt ccccttttct atcacatcgg cattagcctt
     7321 tatatgacta ttccttaatt tattacaagc atccagagag atcttgattt catccatcaa
     7381 cttagccggg cttagttctt tactgattgg agccgaatag aatccaaaat aattaacacc
     7441 ccccgtagcc tctacaagct tgccttcctg acattcaggc atttcaggac cattcaggtt
     7501 gatcttcaat ttttggctta tcttttcgtt ctcactgaat atcctccctg caacatcacc
     7561 cataagttcc aggccatcag actgaaactt tcgcgagaat ctgccttctt cagaatgtgg
     7621 ttggataaaa ataatattca tgtccagatc agccagtgcc ttgtatatgc tgcccatttc
     7681 attgcctgaa atcaaatatt ccttcttctc tgtttttagc ttgcgcactg tattatttga
     7741 aaaatcagca gcatttccta taatcatcag gatgtttctt tgaccgggtt gaaagccacc
     7801 atgtatctcc aatgtttttt tcaaggcaaa tctgaacaca gtttccctat catcatccaa
     7861 tggacgatag aaaacctcct tatcgatgaa agctgcaaca tcctttctgt tttgagttaa
     7921 ttttctaaag gtaaaaggga cctgattcga ctgaaaatcg cgatagatac cacatcctac
     7981 atttactata ttagctcccg gaaagttggt aggtaatttg tcaataaggt cagctaactg
     8041 ggatttgtag gtattcattt ctgtacttcc ttctatcagg aagaagatat ttactatttt
     8101 attatcctcg cttctttttc tctgagcttc tacaatctta ctatatatgg cggggtcaat
     8161 cgatccggac tcctgtccac tcccaacagt tgttaccttg cctataatgc cggtttggaa
     8221 aaggttgttc cccggattag ccaggattgg atacctcagg atgcttccct tatatctcct
     8281 ggggttagac ttggcaagaa attccgggga aatagccaca gggtcctgac taaagagaat
     8341 attatccttg tctcctgttt taagaaactg attggcatcc cggctattgg caaagaaaga
     8401 aaccctcttg ttttcgtcag cttttctctc ctcacaggcc ataatattat gattgggctc
     8461 cagagcgata cgcgtattcc acttggaaag tcggcttaga tccacccagc caataatatt
     8521 ggttttgggc gagctcccga attgatattc agttgaaagc agggcacggc cattttcctt
     8581 tttcaatatg tagaaaatct cataaatatt cctgtctccc agggtctttt tactgtatgg
     8641 actgtcatat atccttacta tttccttatt actcaatccc accagatttt taatgtcttc
     8701 cgccttattc aacaagatcg ccttcaagga tatcttggac tttgaatcaa ccagacccat
     8761 accccattgc aatacggtac ttctttctac ccagcccata tccactgcta tagctccggc
     8821 cggaatttcc ttgccatgag gtactccacc gtcgatccta aagatatgtg caaatgatcc
     8881 tttgacctca ctgaccgcaa aggactcgag aaagcctaag gttgacttag tcccggaggt
     8941 cacagaaggt ccgttatatg cgatattccc ttcccgatcg ttgattataa tccaggtccg
     9001 tcggcttctt ttatcccctg caggcttgag attttggttg ttgatataca gattcagatc
     9061 attattagcg cctgagaaat aataatccag aggtggcgaa taatcagccg gtgcttccat
     9121 taaggtgata gcctgaccga atgaaagccc tttaactaaa aagcagaaaa cgaatatcga
     9181 aaagtatctt tttatcattg atgtcaattt agtacgtaat aaattcctat ccttccgcct
     9241 actgagtggt aaatctttgt ttgatagaag aaggaagcat cacttatcgg ggtaaatgtc
     9301 ctgttgctac tatcatatga ctctatcaac tgtggatttt cagtaacgat caccggcata
     9361 agccccagtg taaagtcaag tccgaagaca aatccgaatc ctcgcttacc ttgccacgat
     9421 ttactctcat atacataggt agggctcaat ctggcagcaa gggtaaatct attttttaag
     9481 gtcaggttct tatctatctt gactgctgtg atctcacaat tgtctatact ggaatcatca
     9541 ccataatagg aataaattga agcagaattg gtgctaaccg tcctggtcga tgagaagatg
     9601 taacctggca ttatgttggc ttgtaccatg aatactcttg ggtcattacc tctgccaaag
     9661 ttatacacca agccaatcgg cgcataaaca ccaaccacat tggttgatat ttgtcctgcc
     9721 tgcattctga attccgatgt cttcggcgct ctggacagat tattgttgta atgggaactg
     9781 aaagtattca gtttgacatc tgaaccaacg acttcatcct ttaggttatg atatctgaat
     9841 aataaaccta ttgaataaaa caccttattg gaagggctga agcttttcca gtattcacct
     9901 cccagcccga tatgaaatcc cgttgatttg aagtcggact cttgtatgcc tgctgacact
     9961 tcgttgcgag gcatcccgta ccctccatcc agatagacca ttttgaagtc acgtgttttc
    10021 tttttccctt catatgaatt gtaattgtcc tggggttgtg ccatcggctc tctttccttt
    10081 ttttcttgct tttgctttgg ctcctttttc acttttggtg cctccattgc aacatcatgc
    10141 cccccggctc cggctgcaga aaccctaacc aactcgatgg aactaatggc agccgtaagt
    10201 tcttctgaat cttccattga aaatacgatc atatcaaaat ccaatgtaat atcgcgttcc
    10261 ttatttacaa cctgaccggc atccacataa ttttccgtcc ttttgacaac ctgaatcttg
    10321 acaaaataaa ggtcgctttg aagtgctctt atgtctttga cggtcaatcc ggtaatacaa
    10381 aatttaaatc cattgggaaa gtactcggca atttggttga tatatcctat tgccattcct
    10441 tcagtcggat gcaaaagcat atcgttgaga taaggggttg tggaattgcc cttatccaac
    10501 aatttgagga agttggattc acgccagtca tacttttcat catagatatc agcgtacctg
    10561 ttataattgg caagaaattg attcaaggca tctctaatct ttcctttata catttgttcg
    10621 atgcccgacc tgtcaataac ggaccaatct cccttttcca gcttacaatc ctgaccaaaa
    10681 atattggagg agaaaaggat gatgccgaaa atcatccaaa aaatatgtat tctgcggatc
    10741 ttcatattga atctctttta acttttttta atgtataact ataatttttc ctgttacttc
    10801 ggctttgtca ttccccaatc ttacaagata tataccattc ggaatgtcca acagactgac
    10861 ctccgtcagc aagttgctct ctgcttgccc aatcgtcata ctatatatac gcgtgcccat
    10921 catattgtat atatccagcg tttgtttgcc gggagacaaa cctgccgcct ggatatacat
    10981 atgcccggag ccgggattgg gatatgcttt cagacttttt gagtctgtac ctccacaagg
    11041 gattaccact tcatcaccat ctgaacgagt agagataaag gtagaatcac aagaattttt
    11101 acaatcgtaa ccgattgcga tgtatttatt ttttatcagg ttttcctggg ttaccttcag
    11161 ccaggagtta ccggccgaat caattctgat agccttgttc tggtcatcca gtctatacca
    11221 atcataacaa aacccttcct tatgccttgc aagtaaaaac ggtccacatt catcaatgac
    11281 aaattgatca tcggcaaatt ccccactcgt aatcttgctc acaaaatcct tgtccatttt
    11341 agatctacat tctccggcgt cattcttaag cgtatattcc agatttactt tgccggtaat
    11401 aaaataaaat ccactgccca gatcctggat gttagaaggc aatgatgaac cttcaagaga
    11461 atatcccggt tttagcttat agactccgca tttatattca tcaaatatat ctgaagtcgg
    11521 ttcattctgg ataaaagaaa tggtttgaga catttcacac ccagcctgat caaaagtttt
    11581 tacctttaat ttgaagttct ttgtttgttt gttgaaatct attacgatct ctttactgtt
    11641 tcctatgata tcggtatcaa gccaccacgt cgcactggcg taagctttgg gaccaagttc
    11701 tccatttagt ttgaaagtca cttctgtctt ggtaggttgg gtacagaaat tgactatgga
    11761 atcaataggg atgtc
//
LOCUS       DAOQMC010000047        11686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2119_length_11686_cov_4.599519, whole genome shotgun sequence.
ACCESSION   DAOQMC010000047 DAOQMC010000000
VERSION     DAOQMC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2119_length_11686_cov_4.599519"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKV31_03285"
     CDS             <1..198
                     /locus_tag="PKV31_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HNB91908.1"
                     /translation="VARAEGKPEDMLEKIAMGKLNKFYQESTLLNQAFVKGDGKKSVR
                     DYLASIDKDLTVTGFRHVALG"
     gene            320..1036
                     /gene="pyrH"
                     /locus_tag="PKV31_03290"
     CDS             320..1036
                     /gene="pyrH"
                     /locus_tag="PKV31_03290"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNB91909.1"
                     /translation="MELPYKRILLKLSGESLMGGQGFGIESERLEHYATEIRSAIDLG
                     VEVAVVIGGGNIFRGLQAHNSGIERVTGDYMGMLATVINGMAVQSMLETKGVYTRLLS
                     ALEIREVAEPYIRRRATRHLEKGRVVIFSAGTGSPYFTTDSAAALRANEISADVLLKG
                     TRVDGIFSSDPEKDPTATRYSEITFDEVINKNLNVMDMTAFTLCRENSLPIIVFDINT
                     PGNLARLLKGEKVGTLVRNR"
     gene            1114..1503
                     /locus_tag="PKV31_03295"
     CDS             1114..1503
                     /locus_tag="PKV31_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91910.1"
                     /translation="MKQSILLGMVILLYMVFSGGCTKDNMLCTDDGKFCRSIVNSGEE
                     KALAAINSYLSKQETNQADSTKLHLLKDWLECKSCIQEVRILCNSCIETYPTQSELSV
                     RLTGSEPDNVLVLDILMSKPLKAIRFH"
     gene            complement(1678..2703)
                     /gene="galE"
                     /locus_tag="PKV31_03300"
     CDS             complement(1678..2703)
                     /gene="galE"
                     /locus_tag="PKV31_03300"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNB91911.1"
                     /translation="MSKILVTGGCGYIGSHTIVELIESGFEVVCVDDLSNSDGSALQG
                     IKAITGKDVTFYNYDAGDINRMQEVFTREGRIDGIIHFAAFKAVNESVQLPLKYYVNN
                     VSGLANLLQTALKNGCNRFLFSSSCSVYGDADQLPVSESSPLGEIRSPYARTKLICEE
                     ILMDLASSGSPMKSIALRYFNPAGAHPSNEIGESPTINASNLVPVITETAIGKRPGFT
                     VFGQDYDTRDGTCIRDYIHVSDIAAAHALAMKLLLENHYTLPFDIYNLGSATGNTVLE
                     VIDAFVRVNEIRPNHVIGPRRAGDVVAVYADNSKAREQLSWKLRYNLDDIVRTAWNWE
                     KRRSGQN"
     gene            complement(2720..5188)
                     /locus_tag="PKV31_03305"
     CDS             complement(2720..5188)
                     /locus_tag="PKV31_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNB91912.1"
                     /translation="MRFFQTFFLFLLSLTAFSSNYNGEIKTFSQPDGSQVDVRLFGSE
                     FYMRAEGLDGYTLVRDDASKWICYARLSEDGRELIATEYRYFGSQSGGATLNVDPGVP
                     KHLDIRPEYVQEVMNHNAQSLFGLPTVEAVQNSYTTYQRSGEVLQGNLKGLCIIVDFS
                     DEVATLKSEDYIDFCNGLNYVRFGNNGSLRQYYLDISGGLLDYQNVVFGIFRAPRTFQ
                     EYDAMPYAVGAQQILDLALNWIKSTGFDFSTLSTNPDKTIQAINLMYTGNPPSWAKGM
                     WFHQGYYSGFSANGVRSGRYNTSPAKSPLTIGTVVHENGHMICKWPDTYKYDNKSGPD
                     GIGAFDVMCSGGPDANPTLPNPYFMHRIGWLRDTDVTGKNEILTDTANAYAAYSYRNP
                     DIPTEYYMFQNRLKTGRSEGIPDEGLVVWRINENGDNQTTNHQVYVVHQNNNINDHSG
                     ACFRKGRMAEYNDSTTPSSRWWISDIPSGLRLWDFSNKGPVMTYKIGRGPSLTANYLN
                     YDNDSNADGYITGGEEFDLNVRLFNVDAGLSSSSIVTCTPIGTNATKVNVLTASIQPG
                     EIASGDSLDITFRIVAEAALKDFETVEFKIDIEEDGRSFYTYASIVTGTVYIMDSTTI
                     IDCDYNFLDPGGLSTYNNGTTVVKTIFPADSTKKVYVEWLSFDLEESNGCKNDILRIY
                     NGENTSAPLIGKLCGSKLPGNIIADNPSGALTFLFKTDPLNAYQGWHAKVTCVERSAT
                     EQMIHADIRLFPNPAGNVLNISMGAPMSGNIIITDLLGNIIRNRELRGENLIQLSTDG
                     LHSGVYFASIRFGKNLITRKFIVE"
     gene            complement(5452..6558)
                     /locus_tag="PKV31_03310"
     CDS             complement(5452..6558)
                     /locus_tag="PKV31_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNB91913.1"
                     /translation="MRIAVNTRFLLKNKLTGLGHFTLEVVRQLVLNHPDDTFIFLFDR
                     PYDSSFIFAPNIKPIIVHPPARATLLFTIWFEWSIPAILRSSKADVFFSPDNFLSLRT
                     RLPTLLVIHDLAYLHTPDGFKGMQLLYYRYFMPKFARRADRIATVSEATRQDILQQYD
                     IAPDKVEVVGLGLNEGFEFEGITVRQDYFVHLGTIQPRKNVIGLLRAFELYKNRTGRL
                     TKLKFIGGKGWNDEEFYTILDKHSYKQDVELLGYRSNEEISHILNHASALLCVSYFEG
                     FGMPVIEAQRCGCPVIASNTSSLPEASGGAALLVNPSDDNAIADAMKKIMEDSLLRAE
                     LIEKGFNNIKRFSWEKTADAIYSLLQELYKQGVP"
     gene            6750..7937
                     /locus_tag="PKV31_03315"
     CDS             6750..7937
                     /locus_tag="PKV31_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB91914.1"
                     /translation="MKIFDFRTYWKTYVVLIGILVLTVSMLYTAFLAAKLKEGEEKSV
                     QLYALAIQDLAHNSSTDNISDVSLQLTQDFKIPAILTDSKDEIIDAVNFGEHENRDTV
                     FLRKQLHEIKRSGYKPIEIENQFIRQRVYYKNSLIYTYLTYFPYVQLLLLAVFVGMGY
                     LGLSAARRSEQNRLWVGMAKETAHQLGTPISAIVAWIEHLKSMTEGNQDQTEVLNELG
                     NDVKKLERVADRFSKIGSVPVLVPHNIYLLLENIRAYMSRRAPRKVIFTFPEFPGENT
                     LVVDVNAPLFDWVLENLIRNALDAMEGKGEIGAEVYEDADYVYIDVSDTGKGMTSGQA
                     SKIFRPGWTTKSRGWGLGLSLSKRIIEEYHGGKIFVKKTAPGKGTTFTIRLNKNKSSQ
                     PAT"
     gene            complement(8022..9821)
                     /gene="rho"
                     /locus_tag="PKV31_03320"
     CDS             complement(8022..9821)
                     /gene="rho"
                     /locus_tag="PKV31_03320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNB91915.1"
                     /translation="MQLDSMLVPELREVAEKNGLTGYKRLSKQELIYKILDQQALTSQ
                     ISEDKLKAMEAESAKSKPHQKEGLRAERRPVNPIKKENQPETASDETIQESKPAIQAG
                     KKPRIKKSTKVELTGTEAEQTPVSVVENEKTETPPEPPRKKFFTTNPESVTEKDAIHV
                     AVKEERKHKTTEPEASSGAKKFQKTEKAPSNQDTSRPNQVNSESETTVAPKQMEKPEP
                     EGPKFNIDFDGMIAGEGILEMMPDGYGFLRSSDYNYLTSPDDIYVSPSQIKLFGLKTG
                     DTVKGMIRPPKEGEKYFALLRVENINGLLPSQIRDRVPFDYLTPLFPIEKFNLTTNPT
                     GRDYGNRMIDLFTPIGKGQRGLIVAQPKTGKTFLLKDVANAIAANHPECFLIVLLIDE
                     RPEEVTDMKRSVNAEVVASTFDEPADNHVKVAGIVLEKAKRLVECGHDVVILLDSITR
                     LARAYNTVAPASGKVLSGGVEANALHKPKKFFGAARKIENGGSLTILATALIDTGSKM
                     DGVIFEEFKGTGNMELQLDRTLANKRLYPAIDLTKSSTRRDDLLLDKETLQRMFILRN
                     QLADMRPDEAMEFLRKYMFGTISNEEFLTSMNG"
     gene            10281..11561
                     /gene="serS"
                     /locus_tag="PKV31_03325"
     CDS             10281..11561
                     /gene="serS"
                     /locus_tag="PKV31_03325"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNB91916.1"
                     /translation="MLRVEILRNQRDKVVTGLKKRNWSDTDILIIDGIIDLDDKRKEI
                     QTLMDGELAEQNQLAKNIGDLMKNKQAAEAEGLKARVAELKESTKAREENLRDIKQEL
                     EDKLITLPNIPNEDVPFGVGADDNMVYQAWDKPLPVLPEGSLPHWELAEKYNLFDMKL
                     GVKLTGSGFILYRGQGAKLQRALINYFLEYNTAAGYEEILPPHLVNEDSARATTQLPD
                     KEGQMYYCEKDDLYLIPTAEVPVTNIYRDMILSEQEIPGMLTAYTPCFRREAGSYGAH
                     VKGLNRVHQFDKVEIVRVEKPENSYAALDGMIRHVSRLMENLGLPYRILRLCGGDMGF
                     ASAMTYDFEVWSAAQERWLEVSSVSNFETFQSNRLKLRYRDENNNTQLLHTLNGSSLA
                     LARIVAALLENNQSPEGIRIPEVLRNFFGADYIK"
BASE COUNT         3155 a         2448 c         2655 g         3428 t
ORIGIN      
        1 gttgcacgcg ctgaaggtaa gcctgaagac atgttggaaa aaattgccat gggcaaactg
       61 aataaatttt atcaggagag cacattgctc aatcaggcat tcgtgaaggg tgatggaaag
      121 aaatccgtaa gggattatct tgcctccatc gacaaagacc tgacggtgac cggcttcaga
      181 catgtagctc tgggttaaga aaattgaaaa aagcgaatgg aactccattc gcttttttta
      241 tacctgtttg catcctgttt tgaaagtttc gtctccccat tttatcattt tgttcttttt
      301 agtttaaaaa aatcttgaca tggagttgcc ttataagaga atattactga agctgagcgg
      361 tgagtcattg atgggcggac agggatttgg cattgaatct gagcgattgg agcattatgc
      421 cacggaaatc aggtcggcca tcgatctggg tgtggaagtg gcagtcgtca tcggtggtgg
      481 taatattttc agaggtcttc aggcccataa ttcaggtatc gaaagggtta ccggcgatta
      541 tatggggatg ctggccaccg tcatcaatgg tatggcagtg cagagtatgt tggagactaa
      601 aggtgtctat acgagattat tgtctgctct ggagatcaga gaggtggcgg agccttacat
      661 ccgacgacgc gccacacgcc atttggagaa gggtagagtg gttatttttt ctgccgggac
      721 cggtagtcca tactttacaa ctgattcagc agccgctttg cgggccaatg agattagtgc
      781 tgatgtcttg ttaaaaggca ccagggtgga cggcatcttt tcttcagatc ccgaaaaaga
      841 cccgactgct accagatatt ctgaaatcac cttcgatgaa gtgatcaata aaaatctcaa
      901 tgtgatggat atgacggcat ttaccctgtg ccgtgaaaat tcattgccga tcatcgtttt
      961 cgacattaat actcccggta accttgcccg actgctgaaa ggagaaaaag taggtacact
     1021 ggtgagaaac cggtaatgtg caggtttttc ttaacgacat ttccaaccag gtttcaagat
     1081 tgatcgttcc tataataaag gatatttgtg attatgaaac agtcgatatt gcttggaatg
     1141 gtcattttac tttacatggt gttttccggt gggtgcacaa aagataacat gctttgtact
     1201 gatgatggca aattctgccg ttctatcgtc aactccggtg aagaaaaggc tcttgctgcc
     1261 atcaacagtt atttgagcaa gcaggaaacg aatcaggccg acagcaccaa actacacttg
     1321 ctcaaagatt ggctggagtg taagagttgt attcaggagg tccggatatt gtgtaattcc
     1381 tgcattgaaa cataccctac tcaaagcgaa ttgtcggtca ggctgacagg ttcagagcct
     1441 gacaatgtgc tggtgctgga tatccttatg tcgaagccgt tgaaggctat caggtttcat
     1501 tgatttagtt ttcccggata tgggcacagt gcaaattatg gttacgaata aaagtcatgg
     1561 attgcagttt tgactgatgt aagtcgcctt tcgggatcaa aacacaccgt attagatcca
     1621 tatatcacct attggcataa aggtatcttt ttcacttttc aaacttctca ctccacatca
     1681 attttgtccg cttcttcgtt tttcccagtt ccatgcggta cgtacaatgt catcaagatt
     1741 gtatctaagc ttccagctta gttgctccct ggcctttgaa ttgtcggcat atactgccac
     1801 gacatcaccg gcacgccgag gtccgatgac atgattaggc ctgatttcat tgacccgaac
     1861 aaaggcatcg atgacttcaa gaacggtatt accggttgca gagcccaggt tgtagatatc
     1921 gaaaggtagt gtataatgat tttccagcag gagcttcatc gccagggcat gtgctgctgc
     1981 tatgtccgaa acatgaatgt aatcacggat gcaagtgccg tccctggtat cgtaatcctg
     2041 accaaagacc gtaaaaccag gtctcttgcc gatagcagtt tcagttatga ccggtacaag
     2101 atttgaggca ttgatggtgg gcgattcgcc gatttcatta gagggatggg caccggcagg
     2161 attgaaataa cgcaacgcga tggatttcat aggtgaacct gaagatgcca gatccatcag
     2221 gatttcttca cagatcaatt ttgttcgtgc atagggactg cgtatttctc caagaggact
     2281 ggattccgag accggaagtt gatcggcatc gccatagacg ctgcacgagg atgaaaacag
     2341 gaagcggttg cacccatttt tcagagctgt ctgcaggaga ttggccaagc ccgatacatt
     2401 gttgacatag tatttaaggg gcaattgcac ggattcattg accgccttga atgctgcaaa
     2461 gtggatgatg ccatcgattc ttccttcccg tgtaaaaacc tcctgcatcc ggttgatatc
     2521 tcctgcatca taattataga aagtgacatc ttttccggtg attgctttta tcccctgtag
     2581 ggcacttcca tcggaattac ttaaatcatc aacacatacg acttcaaaac ctgactcgat
     2641 gagctctacg attgtgtgac tgcctatata gccacatccg ccggtgacga gtattttcga
     2701 catagataga cctatacgtt tattcaacga taaattttct ggtgatcaaa tttttgccga
     2761 aacggatact ggcaaaatat actccgctat gtagtccgtc agtgctcaac tgaatgagat
     2821 tctctcccct taattccctg tttctgatga tatttccaag taagtcggtt atgatgatat
     2881 tcccggacat cggtgctccc atacttatat tcagcacatt acctgccgga ttagggaata
     2941 accggatgtc tgcatgtatc atttgctccg tagcagaccg ctctacacag gttaccttag
     3001 catgccagcc ctggtatgca ttcaaagggt ccgtcttgaa gagaaaggta agcgcgccgg
     3061 atggattgtc tgcaataata ttgccgggca attttgatcc gcacagcttt ccgataagcg
     3121 gagctgatgt gttttctcca ttgtatatcc tgaggatatc attcttgcat ccgttacttt
     3181 cctctagatc aaaactcaac cattctacat acaccttttt cgttgaatca gcagggaaga
     3241 tggttttaac aaccgtagtt ccgttattat aggtactcaa acccccggga tccaaaaaat
     3301 tataatcaca gtcaattatt gtcgtcgaat ccatgatata taccgttccg gtgacaatgg
     3361 acgcgtatgt gtaaaagctg cggccatctt cttctatgtc aattttgaat tcaaccgttt
     3421 caaagtcttt caaggctgct tcggcaacaa tcctaaaggt gatatcgagg ctgtctccgg
     3481 acgcaatctc tccgggttgg atcgatgctg tcagtacatt aaccttagtg gcgttggtac
     3541 ctatcggtgt gcaagtgacg atggatgacg aagacaggcc ggcatccaca ttaaataacc
     3601 ggacattcaa atcaaattcc tcaccacctg taatataacc atcagcgtta ctatcattgt
     3661 catagttcag gtaattggct gtcaatgaag gaccacgtcc tatcttataa gtcatgaccg
     3721 ggcccttgtt actgaaatcc cacaatctta gtccacttgg aatatcagaa atccaccaac
     3781 gtgatgatgg ggtggtgctg tcgttatatt ccgccattcg gcctttcctg aagcatgcac
     3841 ccgaatggtc gttgatattg ttgttctggt ggacaacata tacctgatgg ttggtggttt
     3901 gattatctcc gttttcattg atgcgccata caaccagacc ttcatcgggt attccctcac
     3961 ttcttccggt tttaagacgg ttttgaaaca tataatactc cgttggaata tccggatttc
     4021 tataggaata tgcggcatat gcattagcag tgtcagtaag gatttcattc tttcctgtga
     4081 cgtctgtatc tctcaaccag ccaatcctat gcatgaagta agggttgggc aatgtcggat
     4141 tagcatcggg tcctccggag cacatgacat cgaatgcgcc aatgccatcc ggaccggatt
     4201 tgttgtcata tttgtatgta tcaggccatt tgcatatcat gtggccgttt tcgtgaacta
     4261 ccgtaccgat ggtcaatgga gatttggcag gtgaggtatt gtatctgccc gatcttacac
     4321 cattagcaga gaaaccacta taatagccct gatgaaacca catgcccttg gcccatgatg
     4381 gcgggttgcc cgtgtacatc aggttgatag cctggatggt tttgtcggga ttggtggata
     4441 gggtggagaa gtcaaatcct gtacttttga tccaattcag tgccaggtcc aatatttgtt
     4501 gggccccgac agcataaggc atggcgtcat attcctggaa ggttctgggt gctctgaaaa
     4561 ttccgaagac gacattttga tagtcgagca gtcctcccga gatatccaga taatactgtc
     4621 tcaaagatcc attattacca aaccggacat agttaagacc gttacagaaa tcaatgtaat
     4681 cctcactctt taaggtagct acttcatccg aaaaatcaac aatgatgcaa agacccttca
     4741 aatttccctg caatacttca cctgatcgct ggtaggttgt gtaactattt tgaacggcct
     4801 ctacggtagg tagtccaaag agcgattgag cgttgtgatt catgacctcc tgcacatatt
     4861 ccggacggat atctaaatgt tttggtacac cgggatcgac attcagcgtt gcacctcctg
     4921 attgagaacc aaaatatctg tattcggtgg ctattaactc tctgccatcc tccgataatc
     4981 gggcataaca aatccattta ctggcatcat ccctgacaag ggtataacca tccaggcctt
     5041 ccgcacgcat ataaaattcg gagccgaaca gcctcacatc gacttgtgaa ccatcgggtt
     5101 gggaaaaggt ctttatttcg ccattataat tggaagaaaa tgcggttagt gaaagaagga
     5161 aaagaaaaaa agtctgaaaa aaacgcatct ggcaatcatt taatttagaa aaaataaaaa
     5221 ttgaaaggta aagatagaaa tgtatctatc aaattgaaag gatattcagt taatttgtaa
     5281 tatagtggtg tgcattgttt cctttcaccc aatgaatttg aaagcaatcg gaaggtcttt
     5341 ccgggtgaaa gaaagtgact cgtgtttcag agaaaatgat ttctgaacca ttgtccgatg
     5401 tgccatcttg tcaatgattg ttgaatgtca gtagcttggg tgtattgacc attatggaac
     5461 accctgtttg tatagctcct gtaatagtga atatatggca tcggctgttt tttcccagga
     5521 gaatctcttg atgttattaa atcccttctc aatcagttcc gctctcaata gactatcctc
     5581 cattattttc ttcatggcat cggctatagc gttgtcatcg gatggattta ccagtaaggc
     5641 agctcctcct gaggcttccg gcaaggatga agtgttggag gctattaccg ggcagccgca
     5701 ccgctgtgct tctatgaccg gcatgccgaa tccttcaaag taggatacac acaataaggc
     5761 ggatgcgtga ttgaggatat gggatatttc ctcatttgac cgataaccaa gcagttccac
     5821 atcctgttta taactatgtt tgtccaggat tgtatagaat tcttcatcat tccacccttt
     5881 gcctccgatg aatttcagtt ttgtcaacct tcctgttcta tttttatata attcaaaggc
     5941 tcttaataga ccgatgacgt tttttctggg ttggatggtg cccaggtgca cgaaatagtc
     6001 ctgcctgaca gtgattccct cgaactcaaa accctcattt aaacctaatc cgacgacttc
     6061 gaccttgtcc ggcgcaatat cgtattgttg aagaatgtct tgtcttgtgg cttcactgac
     6121 agtggctatc ctgtctgcac gacgtgcaaa cttaggcatg aaatacctgt aataaagcag
     6181 ttgcattccc ttaaatccat ccggggtatg gaggtaggcc aggtcgtgga tcacgagtaa
     6241 ggtaggtagt ctggtgcgta atgacaagaa gttgtccggg ctaaaaaaga catcggcttt
     6301 actactccta agaatggccg gtatactcca ttcaaaccag attgtaaaaa gtaaggttgc
     6361 cctagccgga ggatgaacaa taattggttt tatatttgga gcaaatatga aagagctgtc
     6421 atagggtcgg tcaaagagga agataaatgt atcatccgga tgattcaaga ccaattggcg
     6481 aacaacttcc agggtaaaat gacctagccc tgtcagctta ttttttaata aaaatcttgt
     6541 attgacagca atcctcattg taacgcaaag ctaatcagag tttactttaa ttatttaccc
     6601 gggaggatat tttatcacta agaaccaata cgaggtgctt gtggctacat ttaaaatgca
     6661 aataaaacaa aaataaagcc caatgataaa atatttcata catttggaat gttcataact
     6721 cctggtaatt aaacgttgat catcgtttgt tgaaaatatt cgatttcagg acatattgga
     6781 agacttatgt cgtgttgatt ggcatcctgg tgttgaccgt atcgatgctt tatactgctt
     6841 ttttggccgc caagctgaaa gagggtgagg agaagagtgt tcagttatac gctttggcta
     6901 ttcaggacct tgcacataat tcttccactg ataacattag tgacgtgtct cttcagctca
     6961 cacaggactt caagataccg gctattctta cggattctaa agatgagatt atagatgcgg
     7021 tcaatttcgg agagcatgaa aatagagata ccgtattttt aaggaagcaa ttgcatgaaa
     7081 tcaaacgaag tgggtacaag cctattgaga ttgaaaatca atttatccgt caaagggtat
     7141 attacaaaaa ttccctcata tatacttatc ttacttattt cccttatgtt cagttgttgt
     7201 tgctggcggt ttttgtcggc atgggatatc tcggcctgag tgcagcaagg cggtccgagc
     7261 agaacaggtt gtgggtaggt atggccaagg agacggcaca tcaactggga actccgattt
     7321 cagctattgt cgcctggatt gaacacctga agtccatgac cgaaggtaat caggaccaga
     7381 cggaagtatt gaatgaattg ggcaatgatg taaagaaact tgagcgagtg gcagacaggt
     7441 tctccaagat aggttctgtt ccggttcttg ttccgcataa tatttacctt ttgctggaaa
     7501 atatcagagc gtatatgagc aggcgtgccc cccggaaggt tatatttact ttccctgaat
     7561 tcccgggtga aaatactttg gtggtcgatg tcaatgcacc tttgtttgat tgggtactgg
     7621 aaaatctgat ccgcaatgca ctggatgcca tggaggggaa aggtgagatt ggagcagagg
     7681 tatatgaaga tgccgattat gtctatatcg atgtgtctga taccggaaag ggtatgacct
     7741 ccggccaggc atcgaaaata ttccgacccg gatggactac caaatcgcgg ggatggggac
     7801 tcggactgtc tctcagcaag cggatcatcg aagaatacca tggtggaaaa atatttgtca
     7861 agaaaaccgc accgggcaag ggaactacct tcaccatcag gctgaataag aataagtcct
     7921 cccaacctgc cacctgatat ggatttttat ttgccttgaa aagaaaaggc tccttcccgt
     7981 atcaggggca gaagcctttg tattatagac taaaactttt gtcaaccatt catagatgtc
     8041 agaaactctt cgttgctgat tgttccaaac atatactttc gcaggaattc cattgcttca
     8101 tcaggcctca tatcagcaag ttgatttctc aggataaaca tgcgttggag tgtctcttta
     8161 tccagcaaca agtcatcacg ccgcgtactg gatttggtga ggtcaatagc ggggtacaag
     8221 cgcttattgg ccagggttcg gtcgagttgc aattccatgt tacctgttcc tttgaattct
     8281 tcgaagatca ctccatccat tttggatccc gtatcaatca atgctgtggc aagaatggtt
     8341 agtgagccac cgttttctat cttacgggca gcaccgaaaa atttctttgg cttatgcagg
     8401 gcattcgctt ccacaccgcc ggataaaacc ttgccactgg ccggtgctac tgtattatag
     8461 gcacgggcca atcgggtgat ggagtccagg agaatcacta catcatgacc acattccaca
     8521 agtcttttgg ctttctccaa cacgataccg gcgactttaa cgtgattatc tgcaggttcg
     8581 tcgaaagtgg aggccaccac ttcagcattg acgcttcttt tcatatctgt aacctcttca
     8641 ggtctctcat cgatgagcag cacgatgagg aagcattccg gatgattggc ggctatggca
     8701 ttagctacat ccttaagcag gaaagtctta ccggttttcg gctgggcgac gataagtcct
     8761 ctttgcccct ttccgattgg tgtaaagagg tcgatcatcc tgtttccata atccctgccg
     8821 gtcgggttgg ttgtcaggtt gaatttttca attgggaaga gaggagttag atagtcaaat
     8881 ggtaccctgt ccctgatttg acttggcaac agaccattga tattctctac acgcaataat
     8941 gcaaaatatt tttcaccttc cttgggaggc cttatcatcc ctttaactgt atcccctgtc
     9001 ttgaggccga agagcttgat ctgggaagga gatacataga tatcgtccgg tgatgtcaga
     9061 tagttataat ctgaggacct tagaaatccg taaccatcag gcatcatttc gagaattcct
     9121 tctccggcta tcatgccatc aaaatctatg ttgaatttgg gaccttccgg ttccggcttc
     9181 tccatttgtt taggagccac agtggtctct gattctgagt ttacctgatt aggcctggaa
     9241 gtatcctggt ttgaaggcgc tttttccgtt ttttggaatt tttttgctcc tgaggatgct
     9301 tcaggttctg tagttttatg ttttctctct tcttttacgg ctacatgaat agcatctttt
     9361 tctgtaacac tttccggatt ggtagtaaag aacttctttc tgggcggttc tggtggcgtt
     9421 tctgtttttt cattttctac aacactgacc ggagtttgtt ctgcttctgt accagtcagt
     9481 tccactttgg tagatttttt aatccggggt ttctttcctg cctggatagc aggctttgat
     9541 tcctggatag tttcatcaga ggctgtttca ggttgattct cttttttaat cggattgacc
     9601 ggtcttcttt cagcccttag tccttctttt tggtgaggtt tgcttttggc gctttcggcc
     9661 tccatagctt tcagtttgtc ttcggaaatc tggcttgtaa gtgcttgctg gtcaagaatc
     9721 ttatagatga gttcttgctt actgagtctt ttgtagcctg tcaggccgtt tttttcagca
     9781 acctccctta actcgggcac caacatgctg tctaattgca taatgtcata catacgtaaa
     9841 gataaaagtt tttggatttt aacatttaga ggcaggtagc gccgcttaat gttaccggat
     9901 ttgactaccg gattaatttg ataatttaat tcaattaaaa tttctttcaa atacaggcgg
     9961 atcggtacag ggatgagacc tttagcaacg aacaatgtcg atactaagat tcattgccag
    10021 tactggaact ttctatattt ttaagatttt cagattatta caacactcat ccttgaaaca
    10081 atgactgccc atttaaaatg accgatatac gtagtattag attcgggaaa ttaagcggta
    10141 aatcaatatt gctacagtga tgggcgtctt aaagctcttt cagcttgaaa tgatatgcaa
    10201 atgtacatat ttttttttat tcaactatat ttgtgcctta attttaaatt taatataaat
    10261 atttattcaa atccttctct atgttacgcg ttgaaatact tagaaatcaa agagataaag
    10321 ttgttaccgg ccttaaaaaa aggaattggt cagatactga tattctcata attgatggca
    10381 tcattgacct tgatgataaa agaaaagaaa tccagacgct gatggatggt gaattggctg
    10441 aacaaaacca gttggcaaaa aatattggcg acctgatgaa gaataagcag gctgcagaag
    10501 cagagggatt gaaagcacgt gtagcagagc tgaaggagtc taccaaggcc agagaagaaa
    10561 atttgcggga tatcaagcag gaattggaag ataagctcat taccttaccc aatattccga
    10621 atgaggacgt ccccttcggt gtcggagcag atgacaatat ggtctatcag gcatgggaca
    10681 agccgttgcc tgtattgccc gaagggtccc tgccgcattg ggaattggct gaaaaatata
    10741 acctttttga tatgaagctc ggggtaaaac tgacgggaag tggcttcata ttgtatcgtg
    10801 gacaaggagc aaaacttcag cgggctctga tcaattattt cctggaatac aatacagccg
    10861 caggatacga agaaattttg cctccgcatc tggtcaatga agactctgcc agagcaacaa
    10921 cccaacttcc cgataaggaa ggccagatgt attattgcga aaaggatgac ttgtacttga
    10981 tcccaacagc tgaagtgccg gtcaccaata tttatagaga tatgatcttg tcagaacagg
    11041 aaattccggg gatgctgacc gcttatactc cttgttttag aagagaagca ggttcttatg
    11101 gcgcacacgt caagggactc aaccgggttc atcagttcga caaggtggag attgttcggg
    11161 tagaaaaacc ggagaactca tacgctgccc tcgatggcat gattcgccat gtatcccgac
    11221 tgatggaaaa cctgggcttg ccttatagga ttcttcgcct atgcggaggt gatatgggct
    11281 tcgcctctgc catgacctat gattttgagg tttggtcggc tgcacaggag cgatggctgg
    11341 aggtcagctc tgtatccaac ttcgagacgt tccaatccaa caggttgaaa ctacgttatc
    11401 gggatgaaaa caataataca caactcttac acacactcaa tggaagctca ctggcactgg
    11461 cccgcatcgt cgctgcactc ctggaaaaca atcaatctcc tgaaggaatc agaattcctg
    11521 aagtgctgcg gaactttttt ggggcagatt acattaaatg aaaaaaaata ttgaatgaat
    11581 agcggttatt tcatgtttat gctcctcggg gcagtgggaa ctgttgtggg catgattttg
    11641 agtgcaagat taaagtcaaa atttcaacac tatagtcagg aaggac
//
LOCUS       DAOQMC010000048        11642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2135_length_11642_cov_4.306896, whole genome shotgun sequence.
ACCESSION   DAOQMC010000048 DAOQMC010000000
VERSION     DAOQMC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_11642_cov_4.306896"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKV31_03330"
     CDS             complement(<1..402)
                     /locus_tag="PKV31_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB91917.1"
                     /translation="MENEKPIPKHIAIAGNIGAGKTSLSALLGKHFDWEVHYEDTEDN
                     PYLSDFYDDMTRWSFNLQIYFLNNRYRQILDIQNGDHTVIQDRTIFEDAHIFAPNLHE
                     MGLMSKRDFDNYFDLFSTMSSQVKAPDLLIYL"
     gene            595..1260
                     /locus_tag="PKV31_03335"
     CDS             595..1260
                     /locus_tag="PKV31_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB91918.1"
                     /translation="MGDQRLYALRDISFKVNRNEYVALMGPSGSGKSTLMNILGCLDT
                     PTSGKYLLNDHDVSHMEDKELAHVRNKEIGFVFQTFNLLPRVSALENVAVPLIYAGVS
                     RKERLERAKVAMDMVGLSDRMDHKPNELSGGQRQRVAIARAIVNDPAIILADEPTGNL
                     DSKTSLEIMAIFEKIHSSGNTIILVTHEPDIAEYAHRIIRLRDGLVENDEQNAHPKRQ
                     LSR"
     gene            complement(1322..2038)
                     /locus_tag="PKV31_03340"
     CDS             complement(1322..2038)
                     /locus_tag="PKV31_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNB91919.1"
                     /translation="MKKSALLFLYVFIGIVCLNAQDAKVKYLLSAEQFSDKLKENPQA
                     TLLDVRTPDEFAKSHLKDAINIDWRSKGFLNDASALPKDRQVYIYCLSGGRSHSAADK
                     LRTQGYTVYEMEGGILQWQKAGLPEVKGDGKKGKDGMSLNEFDKMVSSHPYVLIDFSA
                     PWCGPCKKMKLYIDAIEKEMNGALKVVVINTDENMTLVKKMKIYSIPYLQLYRDGKLK
                     WENMGFTDEKYLREVVNDFK"
     gene            complement(2043..2861)
                     /locus_tag="PKV31_03345"
     CDS             complement(2043..2861)
                     /locus_tag="PKV31_03345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB91920.1"
                     /translation="MKVFLWIGVLIASVPFSMKGEYNVRIYSEKENGEVRYYVDNNEW
                     CPVSVVFDFELKNMKSSKGPEFTVVIPAQSSKFPVTTLKPYKENELYGFKYKTTIILG
                     NVHAVHDQGYVYELPFPPGKTYKIFQGYNGRLSHVGQNALDFSMAVGDSVLAARSGVV
                     VDVVQHNHQSCMDPGCEKFNNYILVYHEDGTFGEYAHIRKDGSLVMPGDSISAGCAIA
                     LSGDVGFTSGPHLHFIVYLPGKKGKKSVKTLFRTQNNTSEYLVEGASYYRTKNN"
     gene            complement(2929..4641)
                     /locus_tag="PKV31_03350"
     CDS             complement(2929..4641)
                     /locus_tag="PKV31_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB91921.1"
                     /translation="MKNLCTFILFMMAYIVTAQKIVVSEIMYNPPESGEDTLEYFEIT
                     NLTANEFKLEGCIVTKGVQDTFEAMDVIAPFGHFVCVKKSSAFSAIFPNIDARQWKSG
                     ALSNSGETIEISDAQGNVLTSVTYGNSKNGWPPAADAGGASQELCDLSKDPAIPANWG
                     ASKTGTGRIVNGKEVFGTPGAANNTECGVVINPNTVIVKNFSFSPSELTIKEGETVTW
                     NFEEGHHNVNGKQSAFPDNPDSFFSGPAADAPFTYSFTFTKVGDYSYQCDPHATSMKG
                     KIHVLPKTPSVSYPVRTIGEISQVDGQGVADSLGRLCEVKGIVYGVNMRATGISTTII
                     DSKRDGIGLFSNSKTFGYTVNEGDEVTVRGKVDQFNGLTQIQIDTMWVNSSGNTIFTP
                     RTTTVLGEEEESDLVMLTGMSLKDPGQWKKGSTFNATITNGTNEFTLRVVNTTTLSQI
                     DAPSGSFTVVGIGGQFDSSNPYTDGYQLLPRKSEDLVPTVSTRNLIGDQVSVFPNPSQ
                     DQLFVSSSLNILKLEVLSETGKWISTESNNSRFVDIHNLLPGNYLLRITTAEGIGVKK
                     FIKL"
     gene            complement(4844..8131)
                     /locus_tag="PKV31_03355"
     CDS             complement(4844..8131)
                     /locus_tag="PKV31_03355"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNB91922.1"
                     /translation="MNFKSTLIAMLMFCVSGLMAQVTTSGMSGVVKDASGQLMIGASV
                     ILTHVPSGTVYGTVTLEDGRYSLLNLRVGGPYSLEVSSVGSETYRSSGIYLTLGDVLV
                     NNVVLQESATVLEGVLITDNRNPVINGDRTGASTNIDRRMMERLPSISRSLTDFTRVT
                     PQANGNSFAGRDGRMNNLQIDGANLNNAFGLSSDPLPGGGNQPISLDAIEEVQVNIAP
                     YDVRQTGFTGAGINAVTKSGSNEVHGSVYAYIRPKSFTGLNVADYKLDESARTSSNIY
                     GARVGGPIIKNKLLYFVSLEYENTETAGNNWLADNGSNTGQPNVTRVKEDDLKRVQDH
                     LKTKYGYDPGAYQGYDNTYNNGNYKALARLDWNINDKHTFTARYSYMLGSNEQGTNGN
                     SGPNPRSGNRRISDKSISFENANYSFKNTVSSVAAELNSRLSNKMSNQLILTYSRIQD
                     TRSTPGKLFPFVDIGDGTSSDKSFSNYISFGTELFSVNNDVINNNVIITDNLTYVLGK
                     NTLTAGLSYQLMSFANSYQRMGSSYYRYNTVDDFLSDAPPIAYGVTYVFDGKDPFARV
                     KFGLAGLYLQDRIALTDKFNLTLGVRADMALFHDDPVFNPSVDTLAFLSPSGKVANYT
                     TAKWPKSVPLFSPRLGFNYDIMGDRTLQLRGGTGIFTGNIPFVWFTNMPTNAGVIQNT
                     FEPVSSAVLAKIDHFEADPKYWPNTLASDFPKSPSNSIPGGLNLIDPDFKMPQVWRSN
                     LGIDIKIPNTPLILSADGTYTKDINGVYQYNVNRNPATQKMSYSGDTRDYWASKGDAQ
                     YNKTKGLNANVPMLTNINEGKAITGTFELKVPYYHGLSASVFYNYTYAVDVTGNPGSA
                     AGSAWSNNYSVNDPNEGLLGYSQFAVPNRVGANLSYKVEYGMFATTFGLYYNGSNGGR
                     YAWTYGNDINGDGVSLDLLYVPKNGNELTFAPLKVGDRTFTPEEQAAAFDKFISSVPE
                     LDDARGGYVKRNSGLLPWANRFDVKIMEDISFGGKNGGRKHTIQLGLDIFNVANLFNS
                     EWGVYQTLNGGSNFNYPLLKVSQSSPSAPTFTMLAVGNELATTPFRNNNSVVSTWAMQ
                     FSARYIF"
     gene            complement(8696..11515)
                     /locus_tag="PKV31_03360"
     CDS             complement(8696..11515)
                     /locus_tag="PKV31_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB91923.1"
                     /translation="MCLFAQKGTLRGNVTDAVTGEPIMFGSVYIHQIGNGMMTDLDGN
                     YSIDLQPGNYDIKFTYIGYKDTVWNDVTLKSDDTITINCVMFPSSVVLDDIVVKAKHN
                     KSTTFALAAIKSKSANVMDGISASTFKAIGDGNAASAVGRVTGVSVQEGKYVYVRGLG
                     DRYSKTTLNRMEIPGLDPDRNTIQIDIFPTQVIDNILVSKSYTADLPADFTGGLIDIA
                     TKDFPNRKYMTFSFGVSYNPRMHFNQSFLSYKGGKTDFLGFDDGSRVLPVKPTERIPF
                     LSEAIANPAVDGKRYTGILESFNSTLAAQRQKSSMDFNLNFSFGNQWTKGHRNLGYNV
                     GLTYQNKTEFFQRAVFNRYGKTADPKVYDLELRSETLGDYGVRDALLAGYAGLTLKTN
                     ASKHTLNFLHIRNGESKAGLFDVVSNNQGTAFTAVQHNLEYSQRSISNAHIQGVYEMD
                     DNRWKLEWNLSPSLAKITDPDIRYTRLRNNNGIWTINTESGIPERSWRFLTEYNYTAM
                     TAATLDYSASKMPGKVMFGGQYSFKDRSYEINNFQIFTNKIELDGNPDHIFLPENLWS
                     DENKGGVTFAPNFLPVNTNKYRSDMSNAAAFVSNELTIRQLKIIIGLRSEYFTQRYTG
                     LNQDRKVFDRQKVLEGIDFFPSANLVWSPVEKQKIRLSYSRTIARPSFKEASYATISD
                     PISGFTFIGGFFPDIDVVTKKIIWDGHLVNTDIDNFDLRWEIFGQGSDYLSVSSFYKL
                     FKNPIEMVQYVQAPNNFQPRNVGNGMVGGAELEYIKDLSFLGNMMTGLSFRSNFMYAF
                     SSIQMAESEYSSRIAYAREGEKIQRSRQMAGQAPYLINAVLSYKHPIHKAELTLAYNV
                     YGRTLQYVGIADKPDIYSRPFHSLNFNANMPVLRDKKATIGVRLTNILHSTRGYVYQS
                     FNTGDHYFSLTRPGMSISFRFSYNL"
BASE COUNT         3393 a         2532 c         2261 g         3456 t
ORIGIN      
        1 cagatagatc agaagatcag gtgccttcac ctgagatgac atagtagaaa acaaatcaaa
       61 atagttatca aagtctcttt ttgacatcaa gcccatctca tgaaggtttg gggcaaaaat
      121 gtgggcatcc tcgaatattg tccgatcttg aatgactgta tgatctccat tttgaatatc
      181 cagaatctgt cggtatcgat tatttagaaa atatatctga aggttgaagg accatcgagt
      241 catatcatca taaaaatcag acaaataagg attgtcttct gtatcctcat agtggacttc
      301 ccagtcgaaa tgcttaccca acagagcgga aagggacgtt ttaccggcac cgatattccc
      361 ggcaattgca atatgttttg gaattggttt ctcattttcc atctgtcaaa tttacttcca
      421 gaaaatgggt ttgcaaagca aaaggtgatt tttgacgata attttttgat tcacatcgaa
      481 taatcctggt tttattgaga tttcaaacta atattgcaga taaaagcaat caacttgaac
      541 gatttgaata cgcaaaatgt catagaatta gaacatcttt ccagggtcta tatcatggga
      601 gaccaacgac tttacgcttt gcgggacatt agtttcaaag tcaaccgaaa tgaatatgtg
      661 gcactcatgg gcccatcagg gtcaggtaaa tccactttga tgaatattct tggctgtctc
      721 gatacaccca catcaggcaa gtatctgctg aatgatcacg atgtaagcca tatggaagat
      781 aaggaactcg ctcatgtgcg gaacaaggaa ataggctttg tctttcagac tttcaatttg
      841 ctgcccaggg tttcggcact ggagaatgtt gccgtaccat tgatatatgc aggtgtatcc
      901 aggaaggaaa gacttgaaag ggctaaggta gcaatggata tggtagggct ttccgacagg
      961 atggaccaca agccgaatga gctttcagga ggacaaagac aacgggttgc cattgcccgt
     1021 gcgatagtca atgatccggc aattatactt gcagatgagc ctaccggtaa tcttgactca
     1081 aagacttctc ttgaaatcat ggctatcttt gaaaagatcc attcatccgg caacaccatc
     1141 attctagtga ctcacgaacc ggatatcgcc gaatatgctc atagaatcat tcgtttgcga
     1201 gatggattag tcgaaaatga cgaacagaat gctcatccaa aaagacagtt atcccgataa
     1261 gtaaatttac taataaaatt taaaaggtaa tctgtgactt aacaatcatt tggactgata
     1321 atcacttaaa gtcattgaca acctccctta ggtatttctc atctgtaaag cccatattct
     1381 cccatttcaa ttttccatca cgatacagtt gcaggtaagg gatggagtat attttcattt
     1441 tcttaaccaa agtcatattt tcatcggtat taatcacaac gactttaaga gctccattca
     1501 tttccttttc tattgcatca atatacagct tcattttttt gcaaggtccg caccagggag
     1561 cgctgaaatc aatcaggaca taagggtgtg aagaaaccat tttatcaaat tcattgagtg
     1621 acatcccatc ctttcctttc ttaccatctc cctttacttc cggaagtccg gctttctgcc
     1681 attgaaggat accgccttcc atttcataaa ccgtataacc ttgtgtccgc aatttatcgg
     1741 ctgcagaatg actacgaccg ccactaaggc agtatatata tacctgacga tccttcggaa
     1801 gtgcagacgc atcatttaaa aatcccttac tccgccaatc aatgttgatc gcatccttta
     1861 aatgactctt ggcaaattcg tcgggagtac ggacatccaa aagagtagcc tgtgggttct
     1921 ccttaagctt gtcggaaaat tgttcagcac tcagaagata ttttacttta gcatcctggg
     1981 cattcaggca gacaattcct ataaatacat aaaggaacag aagtgcggat tttttcattt
     2041 tatcaattgt tttttgtcct ataatatgat gctccctcca ccagatattc cgatgtatta
     2101 ttctgggtgc gaaaaagggt cttgacactt ttcttacctt ttttgccggg caaatataca
     2161 ataaaatgaa gatgtgggcc tgacgtaaat ccaacatctc cactcaaggc aatggcacat
     2221 ccggcgctga ttgagtctcc gggcatcaca aggctcccat ccttcctgat atgggcatat
     2281 tcaccaaaag taccatcctc atgatagaca aggatataat tattgaactt ctcgcaaccg
     2341 ggatccatgc aactttgatg gttgtgttgg acaacatcaa caacaacgcc tgacctggcc
     2401 gccagaacag aatcgcctac agccatagaa aaatccaacg cattctggcc aacatgtgat
     2461 aatctgccat tgtatccctg aaatattttg taggttttac cgggtggaaa gggtaattca
     2521 tatacatatc cctgatcatg cactgcatga acattgccta agataattgt agttttgtac
     2581 ttaaagccat ataattcatt ttctttgtaa ggtttcaaag tagttacagg aaacttactg
     2641 gattgtgccg ggatgacaac agtaaattca ggtcctttag acgatttcat attcttaagt
     2701 tcgaaatcga aaaccaccga caccggacac cactcgttat tatcaacgta gtatctgact
     2761 tccccatttt ctttttcaga atatattctg acattatatt cgcctttcat tgaaaatggc
     2821 acagaagcaa tcaaaacacc aatccaaaga aataccttca taaaacataa gctacattcg
     2881 aatgataacc cgaatgcagc ttaaaatatt attattctaa aaataattct atagttttat
     2941 gaatttctta acgccaatac cctccgcagt ggtaattctc agcaagtagt ttccgggcaa
     3001 caaattatga atgtcaacaa aacgggaatt gttactttct gtacttatcc attttccggt
     3061 ttcgctgagt acctctaatt ttaaaatatt cagactactg ctcacaaaca gctgatcttg
     3121 agaaggattg ggaaaaacac tcacttgatc gccaattaaa tttctagtac ttacggtagg
     3181 tacaagatcc tcactctttc gtgggagtaa ctgatatcca tcagtataag ggttggatga
     3241 atcaaactgt ccgccaatac ccactacagt aaaggatccg gatggagcgt cgatttgaga
     3301 aagggttgtc gtattcacta ccctcagggt aaattcattt gttccgttgg ttatcgtagc
     3361 attgaatgta gagcctttct tccattgacc gggatccttg agcgacatac ctgtcaacat
     3421 gacgagatca gattcttctt cctctcccag aactgttgtc gtcctgggcg tgaagattgt
     3481 attcccggat gaattgaccc acattgtatc aatctgaatt tgagtcaggc cgttgaactg
     3541 gtcgaccttc cctctgactg taacttcatc accttcattc acagtatatc caaatgtttt
     3601 gctgttgctg aataatccga ttccgtccct ttttgagtca ataatagttg tgctaatacc
     3661 ggttgcccgc atgtttactc catagacgat tccctttact tcacagagtc ttccaaggga
     3721 atcagcgaca ccctgtccgt caacctgact aatctcccca attgtcctaa ccggatagga
     3781 tacagaaggt gtcttcggca aaacgtggat tttacccttc atcgaagtcg cgtgcggatc
     3841 gcattgataa ctataatcac ctaccttggt aaaggtaaag ctataagtaa atggcgcgtc
     3901 agcagcagga ccgctgaaaa aggaatcggg attatccgga aaggcagatt gcttaccatt
     3961 gacattatgg tgtccttcct caaagttcca tgtaaccgtt tctccctctt taatagtcag
     4021 ttcagaaggt gaaaatgaaa aattctttac tataacagta ttgggattga ttacaacacc
     4081 acattctgta ttatttgcgg ccccgggtgt tccgaatact tctttaccgt taacaattct
     4141 accggtacct gtctttgaag cgccccaatt agccggaata gccggatcct tgctcagatc
     4201 gcataattct tgcgatgcac cacctgcatc ggccgcaggg ggccagccat ttttactatt
     4261 gccatatgtg acagaagtca gtacattacc ctgagcatct gaaatctcaa tagtttctcc
     4321 actgttgctt aatgctccac ttttccattg tctggcatct atattcggga aaatagcgct
     4381 gaaggccgaa gatttcttaa cacatacaaa atgaccgaag ggagcaatca catccatagc
     4441 ctcgaaggta tcctgcacgc cttttgtaac gatacatcct tctaatttga attcatttgc
     4501 ggtcagattg gtaatttcaa aatattccaa tgtatcctca ccggattcag gcggattata
     4561 cataatttcc gaaaccacaa tcttttgagc tgttacgatg tatgccatca tgaatagaat
     4621 aaatgtgcat aaatttttca taaaattaaa atttttgaat ttctgaaagt gataggtgaa
     4681 ttaccgggct atgcgaaatt tctaatgagt aaaaataaga caatttataa attagtttgt
     4741 taaaattatt acgctacccc aaaaaaaaga gaccatgcca attgcatggt ccctcaaatc
     4801 cattaatcta tatgaatgaa ctgacaagaa atttactctt atatcaaaag atatatctcg
     4861 cactgaactg cattgcccaa gtacttacta cactgttgtt gttcctaaat ggagtggtgg
     4921 caagttcatt ccctactgca agcatagtaa atgttggagc gcttggtgaa gactggctca
     4981 ctttcaacaa tggatagttg aaatttgatc caccattcaa ggtctgataa acgccccatt
     5041 cagagttgaa caggttagcc acattgaaga tatcaagccc aagttgaatg gtatgctttc
     5101 gtcccccatt cttacctccg aaggaaatat cctccatgat tttgacatca aacctattgg
     5161 cccaaggcaa caagccgctg ttacgcttaa cataaccacc cctcgcatca tcaagctcag
     5221 gaacagaact gataaacttg tcaaaagcag cagcttgttc ttctggtgtg aatgtcctgt
     5281 cacccacctt cagtggtgca aatgtcaatt cattaccatt ctttggcaca taaagcaaat
     5341 caagactcac tccatcacca ttgatgtcat tgccataagt ccaggcataa cgacctccat
     5401 ttgaaccgtt ataatacaaa ccgaacgttg ttgcaaacat accatactcc accttgtagc
     5461 ttaaattagc tccgaccctg tttggcactg caaactgtga gtatccgagc aagccttcat
     5521 tcggatcatt gacagaatag ttgttactcc atgcagaacc ggctgcagaa ccggggttgc
     5581 cggtaacatc cactgcatag gtatagttat aaaatacaga agcagaaaga ccatgatagt
     5641 aaggcacttt tagttcgaaa gtaccagtaa tagcctttcc ttcattgata ttggtcaaca
     5701 tcggcacatt agcattaagt cctttagtct tgttgtactg tgcgtctccc tttgaagccc
     5761 aatagtctct tgtatcgcct gaatagctca ttttttgagt ggccggattt ctgttgacgt
     5821 tgtattgata tacaccatta atgtccttgg tatacgttcc atcagcactt aagatcaatg
     5881 gcgtattcgg aatcttgata tcaataccca gattactcct ccatacttga ggcattttaa
     5941 aatcaggatc gatcaaatta agacctcccg gaatggaatt acttgggctc ttcggaaagt
     6001 cacttgccaa agtattcggc caatacttag gatcggcttc aaaatggtca atcttggcaa
     6061 gaactgctga actaacaggt tcaaatgtat tctggattac accggcattg gttggcatat
     6121 tggtaaacca tacgaacgga atgttacctg taaatatccc ggtcccacct ctcagctgaa
     6181 gagtcctgtc acccataata tcatagttga atccaagtct gggtgagaac aaaggaacag
     6241 atttcggcca cttagcagtg gtatagttag caacctttcc acttggactt aggaatgcaa
     6301 gtgtatccac agaaggatta aaaacaggat catcatggaa aagagccata tcagcccgga
     6361 cgcccaaagt cagattgaat ttgtcggtaa gtgcgatcct atcctgtaaa tacaaacctg
     6421 ccaaaccaaa tttgactctt gcaaatggat cttttccatc aaaaacataa gtcacaccat
     6481 aagctatcgg tggtgcgtca ctcaggaagt catccacagt attgtatcgg tagtatgatg
     6541 accccatacg ctgataacta ttggcaaaag acattaactg atagctaaga cctgccgtaa
     6601 gggtattttt acccaaaaca taagtcaggt tatcggtaat tatgacatta ttgttgatta
     6661 catcattatt gaccgagaac agttccgtac cgaaacttat atagttgctg aaacttttgt
     6721 cagaggatgt accatcccct atatcaacaa acggaaacaa tttgcccggt gtggatcgtg
     6781 tatcctggat acggctataa gtcaaaatca actggttgct catcttgtta gacagtcttg
     6841 agttcaattc tgcggctact gaactaactg tatttttgaa tgaataattg gcattttcaa
     6901 aagaaatgga tttatcggaa atccttctat taccggagcg tgggttgggt cctgaatttc
     6961 cgttggtgcc ttgctcatta gagcctaaca tataggaata acgcgctgtg aaagtgtgtt
     7021 tatcattgat attccaatct aatcttgcaa gagccttata attaccatta ttataggtat
     7081 tatcataacc ctgataggct cccggatcat atccgtactt tgtcttaaga tgatcctgca
     7141 ctcgcttcaa atcatcctct tttacccgag ttacgttagg ctggccggta ttggatccat
     7201 tatccgcaag ccagttgtta cctgcagtct cagtattttc atactccaaa cttacaaaat
     7261 atagcaattt atttttaatg ataggtccac ccacgcgtgc gccataaata ttggaactcg
     7321 ttctggcact ttcatccaat ttatagtcag ctacattgag accggtaaaa cttttaggcc
     7381 tgatgtaggc atatactgat ccatggactt cattacttcc acttttggta actgcattga
     7441 taccggcacc ggtaaaaccg gtctgtctta catcgtaagg tgcaatatta acttgaactt
     7501 cttcgattgc atccagcgaa attggctgat taccaccgcc cggcaaagga tcactactca
     7561 aaccaaaggc attattcaga ttagctccat caatctgaag gttgttcatt cggccatctc
     7621 gtcctgcaaa gctatttcca ttcgcttgtg gcgtaactcg ggtaaaatca gttaaacttc
     7681 tgctgatgga aggcaggcgc tccatcatcc ttctgtcaat attggtggac gctcccgtcc
     7741 tgtctccatt tataaccgga tttctgttgt ctgttatcaa tacgccttca agaactgttg
     7801 cactttcttg cagaacaacg ttattgacca atacatctcc caaggtaagg taaatgccac
     7861 ttgacctgta ggtttcagac ccaacggaag aaacttccag agaatacggg cctccgacac
     7921 ggagattgag caagctataa cgcccatctt ccaaggttac tgttccataa actgtcccgg
     7981 aaggaacgtg agttagaata accgatgccc cgatcataag ctgtccggat gcatccttta
     8041 cgacacctga catacctgaa gttgtcacct gggccatcag accggagaca cagaacatca
     8101 gcatcgcgat tagtgtactt ttaaaattca tcgttactat ttttggttca aaaaaatgct
     8161 taatagatgc caaatatact gactagagtt aagttaatgt taaataacct atatattttt
     8221 ttttcatata aagcaaatga tattatctca acgttttata tatattgatg tatttaaatt
     8281 tatagaaaga agcccccgga cctactatca tatcccatcg aagattttat tgaaatcaag
     8341 gtcagaaagc acttttaatc atgtaatcaa ttaactcacc tcgagatcat gcgatattga
     8401 gattaaaatt tgcccataat gctttggcat tctatatgac acaaagcttc ttatgtaata
     8461 aatgcccgca tattccctaa aagacattga tatagaaggc aaggtagaat ctatttcctg
     8521 tataataggc gactgtaggt atttgcaatt cacttttatc tcattttatg ctatatatat
     8581 tttttaaaag tgcctgattg gccaaatatt tcctatcata catgtagttt gtttatttgc
     8641 tctacaccta aataccataa actccggtca cgtttgacta tacatttttc caactttaaa
     8701 gattataact aaatctaaag cttatcgaca ttccgggacg tgtcaatgag aaataatggt
     8761 ctcctgtatt aaatgactga tatacatatc ctctggtgga atgcaggata ttggtcagtc
     8821 tgacaccaat agtcgccttc ttatctctga gtaccggcat attggcattg aaattgaggc
     8881 tgtggaatgg tcgggaataa atatccggct tatctgctat tcccacatat tgaagcgtcc
     8941 gcccatagac attatatgcg agagtcagtt cggctttatg gatgggatgt ttataggaaa
     9001 gaacggcatt aatcagatag ggagcctgac ctgccatctg tcttgaccgt tgaattttct
     9061 cgccttcacg tgcgtaagct atccttgaac tgtattcgga ttctgccatc tgaatagaag
     9121 aaaaggcata cataaaattg cttcggaagc taaggccggt catcatattg cccagaaaag
     9181 acaggtcttt gatatactcc agttcagcac ctccgaccat gccatttcca acattgcgag
     9241 gctggaagtt atttggtgcc tgcacgtact gtaccatttc gataggattt ttaaataatt
     9301 tatagaaact gcttacggaa aggtagtcag agccctgccc gaatatctcc catcgcaggt
     9361 caaaattatc aatgtcggta ttgaccaggt gtccgtccca aatgattttc tttgttacga
     9421 catcaatatc agggaaaaag ccacctatga aggtaaagcc ggaaatcggg tcagaaatcg
     9481 tagcatagga agcttcctta aaggaagggc gggcaatggt gcgtgaatag gataaccgga
     9541 ttttttgttt ctcgacgggt gaccagacaa gattagcaga agggaagaaa tctatacctt
     9601 ccagtacctt ttgtctgtca aacaccttcc tgtcctgatt gagtcctgta tatcgctgtg
     9661 taaagtactc cgagcgaagt ccgataataa tttttaattg tcttatcgtg agttcattag
     9721 atacaaaggc agcagcattg gacatatcag accgatactt attggtattg accgggagga
     9781 agttgggagc aaaggtcaca ccacccttat tttcgtcaga ccaaaggttc tcgggtaaaa
     9841 agatgtggtc agggtttccg tcgagttcaa ttttattggt gaagatctga aaattgttga
     9901 tttcataact cctgtcttta aaggaatact gaccgccaaa catgactttt ccgggcattt
     9961 ttgaagcaga ataatccagg gtagctgccg tcatggctgt ataattgtat tctgtcagaa
    10021 accgccagct cctttcgggg atacctgatt cggtgttgat ggtccatata ccgttgttgt
    10081 ttctcagtcg ggtgtatctg atatccggat ctgtgatttt ggcaagactt ggtgaaaggt
    10141 tccattcgag cttccacctg ttgtcatcca tttcataaac tccctgaata tgagcattgc
    10201 ttatggagcg ctgactatat tcgagattgt gctgtacagc tgtgaatgcc gtgccctgat
    10261 tgttgctgac tacatcaaac aaaccggctt ttgattcacc attccggata tgaagaaaat
    10321 tcagagtgtg tttggaagca tttgttttca atgtcagacc ggcatagcct gccaataagg
    10381 cgtctcttac accgtagtca cctaaagttt ctgacctcag ctccaggtca tagactttcg
    10441 gatctgcggt cttgccgtaa cggttaaaaa cagcgcgctg gaaaaattct gttttattct
    10501 gataggtaag tccgacgtta tagcccagat ttctatgacc tttagtccat tggttgccga
    10561 aggagaagtt cagattgaaa tccatgctgc ttttctgccg ttgagctgca agtgttgagt
    10621 taaaactttc taggattccc gtatatcttt taccatctac agccggatta gcaattgctt
    10681 ctgacaaaaa cggaatccgt tctgtcggct ttacgggtag gacccggcta ccgtcatcaa
    10741 atcccagaaa atctgtctta ccgcccttat aactcagaaa gctctgattg aaatgcatgc
    10801 gtggattata gctgacaccg aaagagaagg tcatgtattt tctattcgga aaatccttgg
    10861 tagcgatgtc tatcaatccg cccgtaaagt ctgccggcaa gtcagccgta taagatttcg
    10921 aaaccagaat attgtcaatc acctgagtgg gaaagatatc tatctgtatg gtatttctgt
    10981 cgggatcaag tccgggaatt tccattctgt tgagtgttgt tttggaatat cggtcaccca
    11041 gtccgcgaac gtacacatac ttgccttcct gaaccgaaac acccgtcaca cggcccacgg
    11101 cagaggctgc attgccgtca ccgatagcct tgaatgtaga ggctgaaata ccgtccatca
    11161 cattggctga tttacttttt atggctgcca gggcaaaggt tgtactcttg ttgtgttttg
    11221 ccttgactac aatatcatcc agaaccacgc tggacggaaa cattacacag ttgatcgtaa
    11281 tggtgtcatc cgattttaaa gtgacatcgt tccatacggt atctttataa ccgatatagg
    11341 taaacttaat atcgtaatta cccggctgaa gatcaatgga ataattaccg tcaaggtccg
    11401 tcatcatgcc gttgcctatt tgatgaatgt aaaccgagcc aaacataata ggttctccgg
    11461 tcactgcatc cgttacatta cctctcaggg tgcctttctg agcgaaaaga cacataggaa
    11521 gcagaattag tatacataaa ttataaatac ttttcactat aaaaaaattt taagaacaca
    11581 ttccctgcac tgtgaaagga gggaatgtgt gcatgaaaaa aaaataatat cgttattctt
    11641 tt
//
LOCUS       DAOQMC010000049        11605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2148_length_11605_cov_4.358961, whole genome shotgun sequence.
ACCESSION   DAOQMC010000049 DAOQMC010000000
VERSION     DAOQMC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_11605_cov_4.358961"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1777
                     /gene="pgi"
                     /locus_tag="PKV31_03365"
     CDS             125..1777
                     /gene="pgi"
                     /locus_tag="PKV31_03365"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNB91924.1"
                     /translation="MPLFKINPANTSSWKSLSSSQKRLEGVDIKTLFSADENRFDNYS
                     ISFGDIMFDYSKNRIDGQVMAELLGLAEECKVRESIDAMFGGEKINETEGRSVLHIAL
                     RNRSDRPILSNGNDVMPEIRRVLSKMKTFCHKIHSGAHKGFTGKKIKHIVNIGIGGSD
                     LGPVMVTEALRPYWLEGIQAHFVSNIDGTHIAEVLKVINPETTLFLIASKTFTTQETM
                     TNAATARDWFLSKGGSSQDIALHFAALSTNEKAVEAFGINTENMFEFWDWVGGRYSLW
                     SAIGLSIALTIGFDHFEQLLEGGYSVDEHFKNTVFEKNIPVIMSLIGIWNCNFLGAHT
                     EAILPYDQYMHRFPAYFQQGNMESNGKSVARDGNEVTVETGPIIWGEPGTNGQHAFYQ
                     LIHQGTRLIPCDFIAPAISQNPLPGHHDKLLSNFFAQTQALAFGKSLEQVKAEMQAAG
                     DSPERIEEIAPFRVFSGNRPTNSFLLKSITPYTLGQLIALYEHKIFVQGVIWNIFSFD
                     QWGVEYGKQLANMILPELQDNATVSQFDSSTNGLINTWKKMK"
     gene            complement(1778..2620)
                     /locus_tag="PKV31_03370"
     CDS             complement(1778..2620)
                     /locus_tag="PKV31_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB91925.1"
                     /translation="MKKLLQLEWLKIYNYKLARIFILLYCLFLFGLILLGTQKFKLGT
                     LEVDLVGQGIFDYPQLWNFTTYFTALIKIFLAFIIITTVVNEFQNRTFKQNIIDGLSR
                     KEFLLSKILTIVILSALSTMLVFVFSLIFGMKYNTATDATYRFAEVGFIVAYFVKLVA
                     FFMLCLFFSVIIRKSAFTFVLLFVWWFAEGIMSGVERAVTHNFTVEDTDNTHRLITSF
                     LPLQSMSNSIPAPIGRLTVIKAITASGGEVVQRIPWEYVATSAVYAVLFGLLSYLLIK
                     KKDW"
     gene            complement(2687..3577)
                     /locus_tag="PKV31_03375"
     CDS             complement(2687..3577)
                     /locus_tag="PKV31_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB91926.1"
                     /translation="MENVLEINNLTKRFGRLTAVDNLSFQVQRGNVYGLLGPNGSGKS
                     TTLGMLLTSINPTAGNWQWFGNVKPTDALKRIGATIERPNFYPYLNATQNLLITAKIK
                     GVAVKNIDEKLELVGLLERKKDKFATYSLGMKQRLAIASAMLNDPEVLILDEPTNGLD
                     PMGIIQIREIISTIATTGTTIILASHLLDEVEKVCSHVIVLRNGKSLYSGEVAGMTNS
                     QPYFELAAADMEALRNALSTIREIESVVLSGKIFHVEVREDLDPGTMNKLAFERGITL
                     THLVKKKNSLETQFLELVKK"
     gene            3958..6546
                     /locus_tag="PKV31_03380"
     CDS             3958..6546
                     /locus_tag="PKV31_03380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB91927.1"
                     /translation="MQGQKTPVIDRDLFFGNPEISSGQLSPDGKWITFMKEYEGIMNI
                     WLKKFDEPFDKARPLTDSKRPLYGYYWTDDAKYILYVKDKDGDENINIFAVSPYEKVE
                     NGKLPESRNLTPFKDIAAQIYATSQKNPDVIMIGINNRDKAWHDLYKLTISTGKLDLM
                     YTNTDRITGYDFDWDDNLRVFYRTDEKGNTSFLYKKGDQLTPIYETSVTEQAYISGWN
                     EDNSKFYLITNKGDLNFQTLYMMDPNTQKLTFIESDPNKKVDFGSLKLDRNTRKIIST
                     SYTADKTEYHWKNKAWEANYNFLKSKFPGREVDFQSSTLDYSKFLISVSGDRYVSEAY
                     FFDTKTKNLIFQYTPRPKLKKLEQYLAPMTPVTYPSSDGLMIPAYLTLPTGKTAKNLP
                     TVILVHGGPKGPRDYWGYNSYVQFLANRGYAVLQPNFRASGGYGKQFLNAGDLQWGKL
                     MQDDVTWGVKYLIENGISDKDKIAIMGGSYGGYATLAGLAFTPDVYACGVDIVGPSNL
                     FTLLESVPAYWESGRAFLYGMTGDPNTEAGKQRIRESSPLFHANNIVKPLLIIQGAND
                     PRVKKAEADQIVIALRDKGKQVEYLLADDEGHGFAKPVNNMAMFAAIEKFLANILGGN
                     YQSSMPEDVAKRLKELTVDINSVTYAPKEEVASAASLPKINNTLKEGTSQYDVNFEVQ
                     GQKIPMQMTRVISRENDLFVVTDEATSAMGNMKDQIKYNADFQPVSRSISQMGQTITM
                     TYTPGKIDVDMSGNKMSLDVKGALLNDGPGMDILVAGLPLKEGYTLVAEMPDITTMKT
                     KQVQLSVTGSEVINGVNHLKVEIASTENTADKTTLWINPATGDASKMTQVIPAMGNAV
                     ITTTRK"
     gene            complement(6635..7822)
                     /gene="kbl"
                     /locus_tag="PKV31_03385"
     CDS             complement(6635..7822)
                     /gene="kbl"
                     /locus_tag="PKV31_03385"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNB91928.1"
                     /translation="MFDSVRPSLREELNDLDAKGLYKRERVIITPQSATIRTDQGGEV
                     LNFCANNYLGLSSHPEVIRAAKEAIDTHGYGMSSVRFICGTQDIHKELEEKTAKFLGQ
                     EDCILYAAAFDANGGVFEPLLGEEDAIISDALNHASIIDGIRLCKAKRFRYINNDMND
                     LETQLKAASGSRRKLIVTDGVFSMDGYIAQLNHITELAAKYDAMVMIDECHATGFMGK
                     TGRGTHEYHHVMDKIDIITGTYGKALGGASGGFTAARQEIVDMLRQKSRPYLFSNSLA
                     PSITGASIKVLDLLSSSTHLRDKLEANTIHFRKGMSASGFDILPGEHPITPIMLYDAP
                     LAQQFANKLLDEGVYVIGFFYPVVPQGKARIRVQISAGHETEHLDRAIAAFTKVGKEL
                     GVI"
     gene            complement(7883..8824)
                     /locus_tag="PKV31_03390"
     CDS             complement(7883..8824)
                     /locus_tag="PKV31_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB91929.1"
                     /translation="MDNKILIIGAGGQIGTELTEALRQIYGVEQVIASDIRENIELPN
                     YLRVDVTDRTALFDCIRANGVTQIYHLAAILSARGEQNPLWAWDINMSGLINVLEAGR
                     ELKLDRVFVPSTIAVFGIEYDRENTGQFAPLIPETVYGISKVAGESWCNYYFKNYGLD
                     VRSVRYPGIIGYKSMPGGGTTDYAVDIYHQAVQGFNFVSFLNKGTILPMMYMDDAIRA
                     TIELMQAPVEKIKIRTSYNIAGCSFAPEDVTRAIQKFYPHFQTTYAPDFRQAIADLWP
                     KRIDDLRAREDWNWHAKYDLELMTADMIKHLAIKYQH"
     gene            8932..10128
                     /locus_tag="PKV31_03395"
     CDS             8932..10128
                     /locus_tag="PKV31_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNB91930.1"
                     /translation="MSKVLIIGAGGVGTVVAHKCAQHKDIFTEIMIASRTKSKCDAIA
                     NSIGADHITTARVDADNVEELNQLFNSFKPAIVINVALPYQDLTIMEACLQSNIAYLD
                     TANYEPKEEAKFEYKWQWAFHDRFAEKGLTAILGCGFDPGVTGVFTAYAAKHYFDEIH
                     ELDIIDANAGSHGKAFATNFNPEINIREVTQKGKYYQDGQWIETEPHEIYKDINYPDI
                     GPKRSYLIYHEELESIVKNYPTLKRARFWMTFSEEYLTHLRVIQNIGMAGIEPVNYMG
                     MEIVPLQFLKAVLPNPGDLGENYTGMTSIGCRIKGIKDGQTRTYYIWNNCSHEAAYKE
                     TGTQGVSYTTGVPAMIGAMMYLKGEWRGNGVFNVEEFNPDPFLEQLNLNGLPWHEKLD
                     IDLEMD"
     gene            10249..11391
                     /gene="nspC"
                     /locus_tag="PKV31_03400"
     CDS             10249..11391
                     /gene="nspC"
                     /locus_tag="PKV31_03400"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNB91931.1"
                     /translation="MTNNPDYSSLTGAAYVLHEDKLIRNLELIRSVKEEAGVSIILAL
                     KGFAMWRMFPLVAEYLDGATASSLFEARLIYEEMGCKAHTYCAAYTDREFDELASISH
                     HMVFNSVNQYLHFREKLKNQYPEISCGLRVNPGFSKVEVDMYNPTAPGSRLGIDAENL
                     KDGLPTGIEGLHFHALCENNSYDLEETLAVFEAKFGHLLHSLKWVNMGGGHLMTREGY
                     DTAHLVRLLRQFKEKYQVEVILEPGSAIAWQTGDLVAHVVDIVDNHGIKTLMTDVSFT
                     AHMPDTLEMPYRPSIVGTVDYKTPYRYRIGGTSCLSGDFMYEYNFENEVKIGSKLIFL
                     DMIHYTIVKTTMFNGVRHPDIYLLHQDGKLECQRRFSYEDYKNRMN"
BASE COUNT         3516 a         2530 c         2424 g         3135 t
ORIGIN      
        1 tatttttccg actaattggt tgtaccagat atcggatttc aatattatct ttatacatcg
       61 gagataatag cttagcttaa acaaccggta aaatagttac attcattttt ttaaatcaat
      121 aataatgcca ttattcaaaa taaacccagc aaatacctcc tcctggaaat ctttgtcttc
      181 atcgcaaaaa cgtttggaag gagtcgatat caaaactctt ttcagcgctg acgaaaacag
      241 gtttgacaac tattccattt ctttcggcga tattatgttc gactattcca aaaacaggat
      301 agacggacag gtcatggcgg aattattggg gcttgctgaa gaatgtaagg ttcgggaaag
      361 catcgatgct atgtttggcg gggagaaaat taacgaaacc gaaggacgaa gtgtattaca
      421 tatcgctctc cgaaacagat cggacagacc tatattgagt aacggaaatg atgtaatgcc
      481 ggaaattcga agagttttgt ctaaaatgaa gactttctgt cacaaaatcc acagtggtgc
      541 gcacaaggga tttacaggta agaaaatcaa gcatatcgtc aatattggca ttggaggtag
      601 tgatctggga cctgtaatgg ttaccgaggc acttcgtcca tattggttag aaggaataca
      661 ggcacacttt gtttctaaca ttgatgggac gcatatcgct gaagtattaa aggtaattaa
      721 tccagaaacg acgcttttct tgattgcttc caagacattt acaacacagg agaccatgac
      781 caacgctgca acagcacggg attggttcct gagcaaaggt ggaagcagtc aggatatcgc
      841 acttcacttt gcagctcttt ccaccaatga gaaagcagtg gaagcttttg gcatcaatac
      901 tgagaatatg ttcgaattct gggattgggt cggtggtcga tatagcctgt ggagcgccat
      961 cggcttgtcc atcgctctga ccatcggatt tgaccacttt gaacaattgc tggaaggtgg
     1021 atattccgtg gatgagcatt tcaagaacac agtgtttgaa aagaatatcc ctgtaatcat
     1081 gtcgcttatc ggtatctgga attgcaactt tttaggtgca cacacagaag ccatcctgcc
     1141 ctatgatcag tatatgcacc gcttccctgc atacttccag caaggtaata tggaaagcaa
     1201 cggtaaatcc gtcgcccgtg atggaaatga agtgactgtg gagacaggac cgatcatctg
     1261 gggcgaaccg ggtaccaacg gtcagcatgc cttctaccaa ctgatccacc agggaaccag
     1321 actcatcccc tgcgacttta ttgcaccagc gatctcacaa aatccattgc ccgggcatca
     1381 cgataagctc ctgagcaatt ttttcgctca aacacaggct ctcgcctttg gaaagagtct
     1441 ggaacaggta aaagcagaga tgcaggcagc aggcgactcc ccggaaagaa tagaagaaat
     1501 cgctcccttc cgcgtcttca gtggcaacag accaaccaac agcttcctct tgaagtcaat
     1561 cactccctac acgctcggtc aactcattgc cctctatgaa cataaaatat ttgtccaggg
     1621 tgtcatctgg aatattttca gttttgatca atggggtgtg gaatacggaa aacaactggc
     1681 aaatatgata ttgcctgaat tgcaagacaa tgcaacagtc agtcaatttg attcctcaac
     1741 caatggcctg atcaatacat ggaaaaaaat gaagtgatta ccagtccttt ttcttgatca
     1801 agagatacga taacaaaccg aataatacgg cgtaaacagc actggtagca acatattccc
     1861 agggaatacg ctgtacgact tctcctcctg aagctgttat tgccttaatc acggtcaatc
     1921 ttccaattgg cgcaggaata gaattggaca tcgactgaag tggaagaaaa ctggtaatca
     1981 atctgtgggt attgtcggtg tcttctactg tgaaattatg cgtaactgcc ctctccactc
     2041 cactcattat accttcggca aaccaccaga caaagagaag tacaaaagta aaagctgatt
     2101 ttctaataat tactgaaaaa aagaggcata acataaagaa agcaaccaac ttgacgaaat
     2161 aggctacaat aaatcccact tcggcaaatc tatatgttgc atctgtagca gtattgtatt
     2221 tcatgccaaa tatcaatgaa aagacaaaga caagcatggt tgataacgca ctcagaatga
     2281 ctatagtaag aattttggat aacagaaact ctttacgact aagcccgtca atgatatttt
     2341 gcttgaatgt gcggttttgg aattcattga ctaccgtggt aataatgatg aaagccagaa
     2401 atattttgat caaggcagta aaataagtgg taaaattcca caattgcgga tagtcaaaaa
     2461 ttccctgccc aacaagatcc acttcaagag tgcccaactt gaacttttgc gtacccaata
     2521 ggataagccc gaatagaaac agacaataca aaaggataaa tattctggcc aatttataat
     2581 tatagatttt cagccactcc aattgcaata attttttcat atatataata atttaataat
     2641 atgattttag ttgtaataca ataagccttc aactatccat gaagtttcac tttttgacca
     2701 attccaaaaa ttgggtttcc agactgttct ttttcttcac caggtgagtc agtgtaatgc
     2761 ctctttcgaa agcaagttta ttcattgtgc ccggatccaa atcttctcta acttctacat
     2821 gaaatatttt tccactcaag actacagatt ctatttcccg aattgtcgaa agtgcattac
     2881 gtaaggcttc catatcagct gctgccaact cgaagtaagg ttggctattg gtcatgccag
     2941 cgacttcacc tgaatacaaa gattttccat ttcgcaaaac gatgacatga gagcacacct
     3001 tttccacctc atccagcaaa tggctcgcca ggatgatggt cgtacctgta gtcgcaattg
     3061 tagaaataat ctctctaatc tgaatgattc ccatgggatc cagaccattg gtcggttcat
     3121 ccagaataag gacttcggga tcgttaagca tggcactcgc tatagccaac cgttgtttca
     3181 tcccaagtga ataggtggca aatttgtcct tcttcctttc taaaagtccg accagttcaa
     3241 gtttttcatc aatattcttt accgccacgc ccttaatctt agccgttatg agcagattct
     3301 gagtggcatt gaggtaggga tagaaattgg gacgctcaat ggtcgcacca atccgcttca
     3361 gggcatctgt aggtttgaca ttaccaaacc actgccagtt gccggctgtc ggattgatcg
     3421 aggtcagcaa cataccaagt gtagtggatt taccactgcc attgggacca agaagaccat
     3481 acacattgcc tcgctgcacc tgaaaggaaa ggttgtcgac tgctgttagc ctgccaaacc
     3541 tcttagtaag attatttatc tcaaggacat tctccataga aattcaaatt tgaaaaacga
     3601 agatactact ttattctccg ggcaactgtt tatacctaaa ttcaactttg ttattaatat
     3661 atataaataa ttattaattt ttataacttt aaatttctat tttttacaca ttataaatta
     3721 ctactatatt attgacacaa ttcctttact atcgataatt ttgctataag ggcataccaa
     3781 taaattcact taataccgaa taatatttat ttggtcgaat aaattgaaaa ttactcctca
     3841 aaatttcaat ttttacaaaa aattaaggga cttttattcc ctcttaaatt atttaatcca
     3901 aattatatga aaaatctttt tttaatctca cttgttttgt tacttcaaac taattttatg
     3961 caaggacaaa aaacgcctgt aatcgacagg gatttatttt ttggtaaccc cgagatttct
     4021 tcaggtcaat taagccccga cggcaaatgg ataaccttta tgaaggaata cgaaggtatc
     4081 atgaatatct ggctcaaaaa atttgacgaa ccctttgaca aggcgagacc gctgactgat
     4141 agtaagaggc cgctgtatgg ctattactgg actgatgatg caaagtatat tctttatgta
     4201 aaggacaagg acggtgatga aaacatcaat atctttgccg tctctccgta tgaaaaggta
     4261 gaaaacggaa agctacccga atcccgcaac ctgacacctt ttaaagatat cgctgcccag
     4321 atttacgcca caagccaaaa aaatccggat gtaatcatga taggtatcaa taatcgggat
     4381 aaagcatggc acgacctgta taaactgact atttccacag gtaaactgga cctgatgtac
     4441 accaataccg acagaattac gggatatgat tttgactggg acgacaatct ccgagttttc
     4501 taccgcactg atgaaaaagg gaacaccagt tttttatata aaaaaggcga tcaactcaca
     4561 cccatctatg aaacttccgt aaccgagcag gcttacattt caggttggaa cgaagacaat
     4621 tccaaatttt atctcatcac caataaaggc gacctgaact tccagacctt gtacatgatg
     4681 gacccaaata ctcaaaaact gacgtttatt gaaagcgatc caaataagaa agtagatttc
     4741 ggatccttaa agcttgacag gaataccaga aaaatcattt ccacgtccta cacagctgat
     4801 aagacggaat atcactggaa aaacaaggcc tgggaagcca actacaattt tctcaaaagt
     4861 aagtttcccg gcagagaagt tgattttcaa agttccacac tggattatag caaattttta
     4921 atctcagtga gcggtgaccg atatgtgtct gaagcctatt tttttgatac aaagaccaaa
     4981 aacctgatct tccagtacac gccacgccca aaactaaaaa aactggaaca atatctggca
     5041 cctatgacgc ctgtcactta tccgagtagt gacgggttga tgataccggc gtatctgact
     5101 ttaccgaccg gaaaaacggc caaaaatctt ccgacagtca tcttagttca cggtggtcca
     5161 aagggtccgc gtgattactg gggatataac tcttatgtac agtttctggc caacagaggt
     5221 tatgccgtcc ttcaacccaa tttccgtgca agcggcggct acggaaaaca atttctcaat
     5281 gccggagacc ttcagtgggg taaactcatg caggatgatg tcacctgggg tgtaaaatat
     5341 ctgatcgaaa atggcatctc cgataaagat aaaattgcca ttatgggtgg aagttacggt
     5401 gggtacgcta cattggcagg tcttgccttt acacccgatg tgtatgcctg tggggtagac
     5461 atcgtcggcc caagcaattt gtttacctta ctggaatctg tacctgccta ttgggaatcc
     5521 ggacgcgcat tcctgtacgg catgacgggc gacccgaata cggaagcagg aaaacaacgc
     5581 atccgggaat ccagcccctt gttccatgcc aataatatcg taaagccgct tcttatcatt
     5641 cagggtgcca atgacccgag ggtaaagaag gctgaagctg atcagatcgt catcgcactg
     5701 agagacaaag gcaaacaggt agaatatctt ctcgccgacg atgaaggaca cggttttgcc
     5761 aagccggtca acaacatggc gatgtttgca gctatagaga agttcctcgc caatatcctc
     5821 ggcggcaatt atcaatcatc tatgccggaa gacgttgcta agcgactaaa agaacttacg
     5881 gtggacataa attcagttac ttatgctcca aaggaagaag tggcatcagc tgcctcactc
     5941 cccaaaataa acaatactct taaggaagga acttcacagt atgacgtcaa ttttgaggtt
     6001 cagggtcaga aaatacctat gcagatgacg agagtcattt caagggaaaa tgacctcttt
     6061 gtcgtcacgg atgaagcgac aagcgcaatg ggcaatatga aggatcagat caaatacaat
     6121 gctgattttc aacccgtatc cagaagcatc tcacaaatgg gtcagacaat tactatgact
     6181 tatactccgg gtaaaataga cgttgacatg agtggcaata aaatgtctct tgatgtgaaa
     6241 ggcgcactgc tcaacgacgg tccgggaatg gatattctgg tagccggtct tccgctgaaa
     6301 gaaggttata ccctagtggc cgaaatgccc gacatcacta ccatgaagac caaacaggtt
     6361 caattgtcag tgacaggctc tgaggtcatc aatggtgtca atcacctcaa agtcgaaata
     6421 gcctctaccg aaaacacggc agataaaaca actctctgga tcaatccggc aacgggggat
     6481 gcaagtaaga tgacgcaggt tattcctgcc atgggcaatg ctgtcataac gaccacgcgc
     6541 aaataaatcg cattaaaaaa ggacagaccg ttaaatctgt ccttttttat tttataactt
     6601 ttatcggaat gtccgatatg cggctttaac taccctatat cacgccaagc tccttaccta
     6661 ccttggtaaa tgcagcaatg gccctgtcga gatgttcggt ttcatgcccg gctgatatct
     6721 gaaccctgat cctggccttt ccttgcggca cgacaggata gaagaatcca atgacataaa
     6781 caccttcatc cagaagctta ttggcaaatt gctgggcgag tggtgcgtca tagagcataa
     6841 tcggtgtaat cggatgttca cccggaagga tatcgaagcc tgaagcgctc atgcccttcc
     6901 tgaagtggat ggtattggct tccagcttgt ccctcagatg ggtggatgaa gacaagaggt
     6961 cgagaacctt gatagatgcg ccggtaatag acggtgcaag actattggaa aacaggtagg
     7021 gtcttgattt ttgtctgagc atatcgacga tttcttgtct cgcagctgta aatccgcctg
     7081 atgctccgcc cagtgccttt ccataggttc ctgtgatgat atctatcttg tccatcacgt
     7141 ggtgatactc atgtgtgcct cgtcctgttt tgcccataaa accggtagca tgacattcgt
     7201 ctatcatgac catcgcatca tacttagctg ccaactcagt gatatgattg agctgggcaa
     7261 tataaccatc catagaaaac acaccatcag taacgataag ttttcgtctt gatccggatg
     7321 ctgccttcag ctgggtctcg aggtcattca tatcattgtt gatgtacctg aatctcttag
     7381 ccttgcaaag tctgataccg tcaatgatag aagcatgatt cagggcatct gagataatgg
     7441 catcttcctc tccgagtaag ggttcaaaca caccaccgtt ggcatcaaag gcagcggcat
     7501 agagaataca atcttcctgt cccagaaatt tggccgtttt ttcttccagt tccttatgaa
     7561 tatcctgtgt accgcagata aaccggacgc tggacattcc gtagccgtgt gtatcaatgg
     7621 cttccttggc tgcacggatt acttccggat gtgaagaaag gccgagatag ttgttggcac
     7681 agaagttcag cacttcgcca ccttgatcgg tacggatagt ggccgattga ggcgtgataa
     7741 tgacccgttc ccgcttatat aagcctttgg cgtcaaggtc gttcaactct tctctcaaag
     7801 aaggtcttac actgtcaaac ataatattat ttggttttta attcatttta aaataagcat
     7861 tcgctgccaa catacattat attcaatgct gatacttaat agccaaatgc tttatcatat
     7921 cagcagtcat caactccagg tcgtatttag catgccagtt ccagtcctct cttgcccgta
     7981 agtcgtctat tctcttaggc cagagatcag ctattgcctg acggaaatca ggtgcataag
     8041 ttgtctgaaa atggggataa aacttttgga ttgcccgggt cacatcttct ggcgcaaagg
     8101 aacagcctgc aatgttgtaa cttgtacgga tttttatttt ttcgacaggt gcctgcatca
     8161 gctcaatggt agcccgtatg gcgtcatcca tatacatcat cggcaggatg gttcctttat
     8221 ttaaaaatga tacaaaatta aatccctgta ctgcctggtg gtagatgtcc actgcatagt
     8281 ccgttgttcc gccaccgggc atgctcttat agcctatgat gcccgggtat ctcaccgacc
     8341 taacatcaag gccataattc ttaaaataat aattacacca gctttctccg gctacttttg
     8401 aaattccgta aactgtctcc ggtatcagag gtgcgaattg cccggtattt tctctgtcat
     8461 actcaatacc gaaaactgca atggtagaag gaacaaagac ccgatccagc tttaactcgc
     8521 gtccggcttc caatacatta attagtcctg acatattaat atcccatgcc catagaggat
     8581 tttgttcgcc acgggctgaa agaatggctg ccaggtgata aatctgggtt actccattgg
     8641 cacggatgca atcaaacaag gctgtcctat ccgtcacatc tactcttaaa taattgggca
     8701 gttcaatatt ctcccggata tcactggcta tcacttgttc caccccatat atctgcctca
     8761 gtgcttctgt caactcagta cctatctggc ctcctgcacc tataattaaa atcttattgt
     8821 ccatagttcc attattggct gcaatataaa aaaaagcact gaaatatttg ataacacctt
     8881 gttgtaacta tctttgtacc gaataaacca gaaccattca aaaattaaaa tatgtcaaaa
     8941 gtattgatca tcggtgccgg aggtgttggc acggttgtag ctcataaatg cgctcaacac
     9001 aaagatatct tcacggaaat catgattgcc agtcgtacca agtccaagtg cgacgcgata
     9061 gcaaattcta taggtgcaga tcacatcacc acagcgcgtg tagatgccga caatgtggaa
     9121 gaactgaatc aattgtttaa tagttttaag cccgccatcg tcatcaatgt agccctgccc
     9181 tatcaggatc tgaccattat ggaggcttgt ctgcagtcca atattgctta tctggataca
     9241 gccaattatg aaccgaaaga agaggctaag tttgaataca agtggcaatg ggcattccac
     9301 gacagatttg ctgaaaaagg tctgaccgct atcctcgggt gcggatttga cccgggtgta
     9361 accggcgtat ttacggcata tgcagccaaa cattattttg acgaaattca cgaactggat
     9421 atcattgatg ccaatgcagg tagtcacggc aaggcatttg caaccaactt caaccctgaa
     9481 atcaacatcc gggaagtcac ccagaaagga aaatattatc aggatggtca gtggatagaa
     9541 accgaaccgc acgaaatata caaagacatc aattatccgg atataggacc taagcgctcc
     9601 tatctaatct atcacgaaga gctggaatct attgtcaaaa actatccgac actaaagaga
     9661 gctcgtttct ggatgacatt cagcgaggaa tatctgacgc acctcagagt aattcagaat
     9721 ataggaatgg caggaattga gccggtaaat tatatgggta tggagatcgt accgcttcag
     9781 tttttgaagg ccgtactacc caatccggga gaccttggcg aaaactacac agggatgaca
     9841 tccatcggtt gccgtatcaa gggaatcaag gacggtcaga cccgtactta ctatatctgg
     9901 aacaactgta gccatgaagc ggcttacaag gagaccggca cacagggagt cagctacaca
     9961 acgggtgtac ctgccatgat cggtgccatg atgtatctca aaggtgaatg gcgaggaaat
    10021 ggtgtgttta atgtcgaaga atttaatccg gatcctttcc tggagcagct caacctgaat
    10081 ggtctgccat ggcacgaaaa attagacatc gaccttgaaa tggattaata aaagccaaaa
    10141 gttgattgta tgaaagcaga atcctcattc tgagatattt cgaaggaatg aggattattt
    10201 tttattgaaa aatgaaatca gaatatcctg aaataaactt ccgtataaat gacaaataat
    10261 cctgactaca gttcgcttac cggtgcagcc tatgtactgc acgaggataa actgatccgc
    10321 aatctggaac ttatccggtc cgtcaaggaa gaggccggtg tgtctattat actggctttg
    10381 aaagggtttg ccatgtggcg gatgtttccc ctggtggccg aatatctgga tggcgctaca
    10441 gccagctcac tttttgaggc acggctcatc tatgaagaaa tgggttgcaa ggctcatact
    10501 tactgtgcag cgtatactga ccgggaattc gacgaattgg cctctatcag tcaccacatg
    10561 gtcttcaact ctgtcaatca gtacctgcat ttcagagaaa agctcaaaaa tcaatatccc
    10621 gaaatcagtt gcggcctaag ggttaatccc ggtttttcaa aggttgaggt ggacatgtat
    10681 aatcctactg cgcctgggtc ccgactgggt attgatgccg aaaatctaaa ggacgggctt
    10741 cccaccggaa tcgagggtct tcattttcat gctttgtgtg aaaataattc ctacgacctg
    10801 gaagagacat tagccgtctt tgaggcaaaa ttcggacact tacttcatag cttaaaatgg
    10861 gtcaatatgg gtggtggcca cctgatgacc agagaaggat atgatacagc tcatctcgtg
    10921 agattgctca ggcaatttaa agaaaaatat caggtagaag tcatcctcga accgggcagt
    10981 gccatagcct ggcagacggg tgatctggtc gcacatgtag tggatatcgt ggacaatcac
    11041 ggcatcaaaa cactcatgac ggatgtatca tttaccgccc acatgcctga caccctcgaa
    11101 atgccatacc ggccgagcat agtcggaaca gtcgattata agactcccta tcgttacaga
    11161 attggtggaa cttcctgtct aagcggtgat tttatgtatg aatataattt tgaaaatgaa
    11221 gtaaaaatag ggtcaaaact gatcttcctc gacatgattc attatactat tgtcaaaaca
    11281 acaatgttca atggtgtacg acatccggat atttatttgt tacatcagga tggaaagttg
    11341 gaatgtcaac gtcgattttc gtatgaggac tataaaaata ggatgaattg agaaacagaa
    11401 gcagaccggt agatttcttg tcaaagttgg acaaattgaa tcaggaaaag tcggcgtaaa
    11461 aacgaagtag gtagataatt aataactgac tcttcaataa atacaaccaa cctaaaaagt
    11521 tgatttagaa tttcagtttt caatcgcgta ttttatttct caatgatcca tgggacccct
    11581 atagaatatg gctttgaagc tatta
//
LOCUS       DAOQMC010000050        11544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2168_length_11544_cov_4.785621, whole genome shotgun sequence.
ACCESSION   DAOQMC010000050 DAOQMC010000000
VERSION     DAOQMC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_11544_cov_4.785621"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKV31_03405"
     CDS             <1..460
                     /locus_tag="PKV31_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNB91932.1"
                     /translation="AGLEYNFDHVLVANSLRAHRIIQKAKEYGLDDMAEEVFFKAYFI
                     DGKNLGDTVALMELGEQAGLTQEQVSDALENDLYAYAVNQDIKEADSLGIRGVPFFVF
                     DRKYGISGAQPVELFVKTLNQAYQEWDQGNTSESIAHTGENACSIDGTCE"
     gene            485..1078
                     /locus_tag="PKV31_03410"
     CDS             485..1078
                     /locus_tag="PKV31_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB91933.1"
                     /translation="MNRISLPADIVVQEKSLLSDNWYHLYRYRFTYTTATGRKINQTR
                     EVYDRGNGASVLLYNEEKNTVILVRQMRFPTWLNGNKEGYLLEAIAGLLDEDDPKECA
                     CRESVEETGYQPINLSKVCSVYVSPGAVTEILHCYVAEYNDILRQGTGGGIDDENIEV
                     LEMDFCDVWNMLLKGEIRDAKTVILLQHLKLSGKMKS"
     gene            complement(1394..2131)
                     /gene="mtgA"
                     /locus_tag="PKV31_03415"
     CDS             complement(1394..2131)
                     /gene="mtgA"
                     /locus_tag="PKV31_03415"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNB91934.1"
                     /translation="MKVKELIGKVRRIHYKRWVYTIVFTFLGLSLVSVVTLRFIPVPV
                     TPLMVIRSVEQLKDKERDFRWEKDWVSLDHISKNAVKAVLASEDQAFFDHNGFDFVAI
                     EKAIAHNKVSTKKIGASTISQQTAKNVFLWQGRNWVRKGLEAYFTVLIELVWPKERIL
                     EAYLNVIEFGDGIYGIEAASKHYFHKSASELNKKEAAMLAAVLPNPLKYKVNAPSDYL
                     SKRCRRIMNQMNTIDYIQSQAQYTLAK"
     gene            complement(2506..2865)
                     /gene="rplS"
                     /locus_tag="PKV31_03420"
     CDS             complement(2506..2865)
                     /gene="rplS"
                     /locus_tag="PKV31_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNB91935.1"
                     /translation="MDAIQFVNEQTLSVNNNMDFGPGDHIEVSYKIIEGAKERTQLFK
                     GDVIQIKGHGLTKSFTVRKISNGVGVERIFPFASPSISGIKIVKRGRVRRARLFYLRD
                     RVGKAARIKEKTSYAKS"
     gene            complement(2984..5206)
                     /locus_tag="PKV31_03425"
     CDS             complement(2984..5206)
                     /locus_tag="PKV31_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB91936.1"
                     /translation="MLFFIFTVIFSSLIITSVQAKHIIGGYISYEFISSTNNINKYQI
                     TLRVYRDCGNPQNGPFDRPAMLSIATSDGKYYRRDFNMGNPVITNLSNLFSNPCITKP
                     PQVCVQEGVYTQTVDLPVDPNRSYYIIYQRCCRNETIFNILNPGEYGATYYTEITPLA
                     QQLQNSSPYFKTIPPIAICAGFNVTFDHSAEDIDGDSLVYHMVTPLNGGGQGGFGAPN
                     CQQVDPQPDCLPPYDSVYYRPPFKGQNPMGGSPTVAIDYKTGLLTGTPNIVGQFVVGV
                     RIDEYRNGVLLSSSIRDFQFNVANCEKVVRAELSVETRKGKDIQFKFCGDQLLKIENI
                     SSLESSIFNYSWEVNYPFGKDTIFSTKDLDLAAFDYGNYFGKMILNKDLQCTDTATFE
                     FTKFPGLHADYTFSYDTCKDEGVRFVSQSYGDTNLPLTLSWADKDGAFGSTTEIDHGF
                     PGSGDYPVRLTITDTNGCVDSVETVIPYYPIPDKVLQWKQIDDCVPAQIVFPSLHPAI
                     DNSYNIEWDFGDGTTGNGLAPMHEYTRVADYDISVHVTDKVGCEYSGLFRRGVRIHDL
                     PTAGFDYSPKELSNIQPVMKISDDSKDAVKWIYYIGNLDSLTEQHPSYTFRDTGLITI
                     YQVVANQFGCEDTISTLVDVVPINTIYMPNAFLPASSGENSEFRPAGNGFGIVEYEMT
                     VFDRFGEIVFRTDEFNQGWSGKNKNGDYLPNGAYGVKVRLLGPRGELKKLSGKAMLIR
                     "
     gene            complement(5420..7657)
                     /locus_tag="PKV31_03430"
     CDS             complement(5420..7657)
                     /locus_tag="PKV31_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB91937.1"
                     /translation="MRHSYILLFVFLLLSVPIQAKHIIGGVMYYEFAGQSGNLITYRI
                     TLKMYRDCKPEANKANFDGLIGEIPALGTIYRGNGVLPIAEINFGTPIVKKLDVVVTN
                     KCLVVPPGICVEEGIYTTTIQLPVSTSSYYIVYQRCCRNNTITNIVRPGDVGSTFMVE
                     ITPSAQSLQNSSPQFKYFPPIAICANFPLEFDHSATDVDGDSLVYSLCAPFVGGGNDD
                     MGTNGCNVVAPQPDCPPPFEEVTFRNPYSPQNPLGGPTPLSIDPATGFLKGTPGISGQ
                     FVVGICLREYRNGNLISEIRRDFQFNVTSCEKTVDAVISATGMIGKDLDIKLCGDKYL
                     DVAFAGQVTNDIFDLQWSFSHNNNVTNGTGKDYRLNTEDLGQYFGTLYLNRGLPCSDS
                     TTIKVNVFPDIRADFTFDYDTCYGKVINFTNMSVSDAGPIISSEWKANGRIFANSFNA
                     QFNTLEPDFYDMILVVSDQNQCIDSVAERVAFFPIPRDELFDPGGVIGCEPFTYQFKK
                     PNSYITDKYIIDWKFGDGQSGSGVSPEHTYQNRGIYTVDVTVTNVFGCTTSATFNNTI
                     TVLQSPVAGFTFEPSNPSNLEPTITIHDESSHASSWLYEFGTGDNSTDRNPIYTYRDT
                     GIYHMVQWVSHENGCRDSLAIIVDVSPRYTLYLPNVINISSNSGNDLYGPVGIPYGVR
                     DFRMAIFDRWGNEVYSTEDFNGRWDGKNKVGDVLPKGVYSVKIDLTEPRGKKKTIYGK
                     AVLIQ"
     gene            7809..8387
                     /locus_tag="PKV31_03435"
     CDS             7809..8387
                     /locus_tag="PKV31_03435"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB91938.1"
                     /translation="MDSSQYEADITACIKTLQTGGLILYPTDTVWGIGCDAENEVAVR
                     KIIRIKNRPDTKSFVVLSQSQSTVREIADPISQLMLAFKNNALRPVTFVLSNARKVAP
                     SVINEDGTIAVRIPSDDFCSNLLKKFGGPIVSTSANISGQPTPSFFQEVNGKIRQEVD
                     YIVRYRQDDMTPRQGSIIIKESPKGQILILRS"
     gene            complement(8371..11163)
                     /gene="fdhF"
                     /locus_tag="PKV31_03440"
     CDS             complement(8371..11163)
                     /gene="fdhF"
                     /locus_tag="PKV31_03440"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNB91939.1"
                     /translation="MKITSSTITEKEIPAGLKGDTGSDEKIAYIDGLPYNMRDGETIL
                     SFIRRNHGYNLVPTLCDAPNLEPFGSCRVCTVDVALTANGSSKAQASCHTPVMANTYI
                     QTNNDRIQKLRKNIVELVLTDHPLTCSGCASNNNCELQDVAARVGISEVRYPDGENHF
                     HYEKDLSHPYMVSDMSLCINCFRCIRACDEVQGEMVLSMAGRAFESKVIKGSDVSYFE
                     SDCVSCGACAQACPTGAINDVFDSRAYAGTDITRTVCTYCGVGCNLEVSSVEGKVKSI
                     RAPYDAEVNAGHTCLKGRYAFSFYNHPDRLTTPMIRRNGELEPVTWDEAYDYIVEKMT
                     AIKTHYGPDAIAGISSSRCTNEENYLMQKFLRVIIGTNNIDNCARVCHSPTAIGMQRT
                     FGTGAATNSIEDLKHTDCIMVIGANPTSAHPVTGAKLKQFMMKDNPSIVIDPRRTVLA
                     RYATHHLQLRPGTNVALLNMMLYYIIKEGLEDKKFIESRTEGYEEFKEHILQLDIDKM
                     EAVTGVEREKVRQAAITYASAPNAMSFHGLGVTEHSQGTYAVMLIADLAMITGNIGRK
                     GVGVNPLRGQNNVQGAADMGCQPYQGAGYMDVTNPEFHKLYEDFYKVKLPIHRGLMIP
                     EMYEASMEDKLKCLWIMGEDVVQSDPNSNFVVEALKKMDLVIIQDIFMNETAKYAHVV
                     LPASSFLEKSGTFTNGERRVQRVNKVVEPLAGTKPDGQIVIELMNRMGGNQPDYNAAT
                     MLEEISQIVPFFAGIKWEDLGINGKQWPVKPDGTDTKILHTEIFKLGKGRFKSADFRE
                     TEELLEHRDKFPYILTTNRNLEHYNAGTMTRRTRNSIILTEDYLMINPVDAEKHFIAD
                     GDMVCVSSPRGKIDVRARVTEDVRPGILSSTFHFPEINMNDLTSDESDSEAMCPEYKV
                     VAVDIRKSKGRFKTEV"
     gene            complement(11221..>11544)
                     /locus_tag="PKV31_03445"
     CDS             complement(11221..>11544)
                     /locus_tag="PKV31_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNB91940.1"
                     /translation="LGHASIVCLPLDFSIMEYLQHLFEFTADESCGKCFPCRLGSKRG
                     AELIEKALKHNYKIDRQLFRDLIETMEIGSLCALGGGVPLPMRNALMYFDDELKTYFK
                     EEFAG"
BASE COUNT         3322 a         2639 c         2279 g         3304 t
ORIGIN      
        1 ggcaggcctt gaatataatt tcgatcatgt acttgttgct aattcgcttc gggcacaccg
       61 cattattcag aaagccaagg aatacggact tgacgacatg gcagaagaag ttttcttcaa
      121 agcctacttt atcgacggaa agaacctcgg cgataccgtg gccttaatgg aacttggcga
      181 acaggcaggg cttacgcaag aacaggtcag cgatgcactg gaaaacgacc tgtatgccta
      241 cgctgtcaat caggacatca aagaagcaga tagccttgga atccgtggag ttcctttctt
      301 tgtttttgac aggaaatacg gtatttcggg agcccagccg gttgaactct ttgtgaagac
      361 cctcaatcag gcttaccagg aatgggatca gggcaataca tccgaatcca ttgcccatac
      421 tggtgaaaat gcctgttcta ttgatggtac ctgcgaataa attatcgcaa taaaataaag
      481 acagttgaac aggatttcat taccagcaga tatagttgtc caagaaaagt cattactttc
      541 cgacaattgg tatcatttgt acagatatcg attcacctat acaaccgcta ccgggcgtaa
      601 aatcaatcag acaagggagg tctatgaccg tggtaatgga gcttctgtgt tattgtacaa
      661 cgaggaaaaa aacacggtca tccttgttcg tcagatgcgc ttccctacct ggctcaatgg
      721 caataaagaa ggatatttat tagaagccat cgcaggcttg ttggatgaag atgatccaaa
      781 agaatgtgcc tgtagggagt cggtagaaga aacaggctat caaccgatca atctttccaa
      841 agtatgttcc gtgtatgtat cgccgggtgc agtcaccgaa attctccact gctatgtagc
      901 agaatataat gatattctac gccagggaac aggaggaggg atagatgacg agaatataga
      961 agtgttggaa atggatttct gtgatgtctg gaatatgctt cttaagggag agatccgtga
     1021 cgcaaagacg gttattttgc tgcagcatct gaaattgtcc ggcaagatga aaagctaaga
     1081 cagttggttt attttcccat ccccgtattt cacagaaaag gcttgactac aatcaatatt
     1141 atctacaaaa gatagaaaag cataaaattt cctctaatgc cttttattgg cttcgaattc
     1201 cccgtctatt ccgaaccggc taacgaatat aattcactga tcaactaaaa aaccggaaaa
     1261 gtgatataac tcagcggact gacctcacta tgaaattcat tctgtcaatt caaattccta
     1321 aaagtattcc atcattcaat agaggaccct tacaatgatg tgatgcaatt tcccaaaggc
     1381 ctcaaaaggc atcttatttt gcaagtgtgt attgtgcctg ggattgaatg tagtcgatgg
     1441 tgttcatctg gttcataatc cttcgacatc ttttggatag atagtcagag ggagcattga
     1501 ccttgtattt aagcggattg ggcaggacag ctgccagcat tgccgcctct ttcttattta
     1561 gttcagaggc acttttatgg aaataatgtt tggaggcagc ctcaatcccg tatatcccat
     1621 ccccgaattc gattacattg agataagctt caagaattct ttccttaggc cacaccagtt
     1681 cgatcaatac ggtaaaatat gcttccagcc cttttctcac ccagtttctt ccctgccaga
     1741 gaaaaacgtt cttggctgtt tgctgggaaa tggtactggc tccgattttt ttggtgctga
     1801 ccttattgtg agcaatagcc ttttctatcg ccacaaagtc aaatccattg tgatcaaaaa
     1861 aagcttgatc ctcacttgcc agaaccgcct taacggcatt tttagaaatg tgatccagcg
     1921 atacccaatc cttttcccac ctgaagtccc gttccttatc tttcaattgt tcaacacttc
     1981 taatgaccat caacggtgtc accggaacag gtatgaatct aagtgtcacc acagatacca
     2041 aactcaatcc gagaaaagtg aacacaatag tatagaccca ccttttatag tgtatccgtc
     2101 taactttccc gatcaattct tttactttca tatgtcaatg ggactgcaat tttaatgcca
     2161 ttccaaacat attataatat tttttaatat attgtttgag ttaatttagg atcttagtca
     2221 tgattcaaag ctgagacatc aaaatattga catacttttc acaaaaaatt gacactttca
     2281 ggtaccaatt tttattatac aacatttcga aagaattgtc ctgtaatttc acccctttga
     2341 gtatggccgc attagctgta tgcacaattt ggaaatataa ctcagatcaa agcctgtttg
     2401 ctatagttct cataaaaaac aattccgggc aaaatataat tcaaaaaagc ataaaaaaaa
     2461 gctgtccccg ggggaacagc ttttataact ttatttacct cagttttaac ttttggcgta
     2521 agaagttttt tccttaatac gggcagcttt acctactctg tctcgcaggt aaaacaaccg
     2581 tgcacgcctc actctacctc ttttgacaat cttaattcct gaaatgctgg gtgaagcaaa
     2641 ggggaagatc ctttccactc cgaccccatt ggaaattttc cttactgtaa aactcttagt
     2701 aaggccatgt cctttaatct gaataacatc tcccttgaag agctgcgttc tttcctttgc
     2761 accttctatg atcttgtagc ttacttcgat gtgatcaccg ggcccaaaat ccatattatt
     2821 gttgacactg agagtctgtt catttacaaa ttgtattgca tccatgacgc taaaattttt
     2881 atatcctttc ttgaaatgag gcacaaaggt aaataattca attgaattaa cctacaatgg
     2941 ccctaaaaat ttgttatttt tttatattgg ctctttacct gaatcatctg atcagcatag
     3001 ccttgcctga cagcttcttc aactcccctc gtggccccag caatctgacc ttgacgccat
     3061 atgcaccatt gggcaaataa tcaccatttt tattctttcc actccaaccc tggttgaatt
     3121 catcggtccg gaatacgatc tctccgaatc ggtcaaacac agtcatttcg tactccacaa
     3181 taccaaaacc atttcccgcc ggcctgaatt cactgttttc gccactcgaa gccggcaaga
     3241 aggcattagg catatagatt gtattaatgg gcaccacatc caccagggtg gaaatcgtat
     3301 cttcacagcc aaactgattg gccactacct gataaatggt aatcaatccg gtatctcgga
     3361 aggtatagct tggatgctgc tcagtcagac tgtcaagatt gcctatataa taaatccatt
     3421 tgacggcatc cttactatca tccgatatct tcataaccgg ctggatattg gacaactcct
     3481 tgggtgagta gtcaaatccg gctgtaggaa ggtcatggat ccttacacct cgcctgaaca
     3541 atccgctata ttcacatccc accttatcag tcacatgaac actgatatca tagtcagcaa
     3601 ccctggtata ttcatgcatg ggggcaagtc cattcccggt cgttccatcc ccaaaatccc
     3661 attctatatt atatgaattg tcaatggccg gatgaaggga aggaaacaca atttgagcag
     3721 gtacacaatc atcaatttgc ttccattgca gcactttatc aggaatcgga tagtagggta
     3781 tgacagtctc tacactgtcc acgcaaccat tggtatccgt tatggtcagc ctcaccggat
     3841 aatctcctga ccccggaaag ccatggtcta tctccgttgt ggaaccaaag gcgccatcct
     3901 tgtctgccca tgaaagtgtc agagggaggt tggtatctcc ataggattgg cttacaaagc
     3961 gtactccttc atctttgcat gtatcataac taaaggtata atcagcatgt aagcccggaa
     4021 atttggtaaa ttcgaatgta gcggtatccg tacattgaag atccttattg agaatcattt
     4081 taccgaaata attaccataa tcaaaagcag caagatccaa gtccttggtg gaaaatatcg
     4141 tatccttgcc aaaaggataa ttcacctccc aggagtaatt gaaaatggac gactccagcg
     4201 aacttatatt ctcaatcttc aaaagttgat ctccacaaaa cttaaattgg atatccttac
     4261 cttttcttgt ctccaccgaa agttcagcac gtaccacttt ctcacaatta gccacattga
     4321 attggaaatc ccgaatggaa gaacttagta agacgccatt gcggtactcg tcaatcctca
     4381 caccaacaac aaactgtccg acaatattgg gtgttccggt caacagcccc gttttgtaat
     4441 caatggcaac agtaggagag ccccccattg gattttgccc cttaaatggt ggcctgtaat
     4501 aaacactatc ataaggaggc aggcaatcag gctgcggatc gacctgctgg caattgggtg
     4561 caccaaatcc tccctgacca cctccattaa gaggggtgac catatgatat acaagactat
     4621 cgccatcaat atcttctgca gaatgatcga aagtaacatt gaatcccgca caaatggcta
     4681 ttggaggtat tgtcttaaaa taaggacttg aattctgcag ttgttgcgcc agtggtgtaa
     4741 tttccgtgta ataggttgcg ccatattctc ccgggttgag gatattgaag atggtttcat
     4801 tgcgacaaca tcgttgataa ataatataat agcttctgtt aggatcgacc ggcagatcga
     4861 ccgtctgagt ataaactccc tcctggacac atacctgggg aggcttagtg atgcatggat
     4921 tactaaaaag attggacaaa ttcgtgatta ccggattccc catattgaaa tcacgtctgt
     4981 aatattttcc gtccgaggtg gcaatcgaaa gcatggccgg cctgtcaaaa ggaccattct
     5041 gagggttgcc gcaatcccga tacacacgta gtgtaatttg atatttattt atgttattcg
     5101 tggaggatat gaattcataa ctgatatatc cacctataat gtgtttggcc tgaactgagg
     5161 taataattaa tgaagagaaa ataactgtaa agataaaaaa aagcactctc ctcattttgt
     5221 taaacgaaat tgaacattga aataatttat aaagaataat tataagaatt tagccgacaa
     5281 gttacgaagt ttttatacga atgtatatca ggcctggatg aaataatcat ttttattatg
     5341 aataaaatca acggagtaaa gtatttataa tttattccct cctgtggaat gacatataat
     5401 gggtctggtt cttccctgat cattggatca atactgcctt gccatatatg gtcttcttct
     5461 tccctcgagg ttcggttaaa tcgatcttta ctgaataaac tcccttaggc agcacatctc
     5521 caaccttatt ttttccgtcc catctaccat taaaatcttc ggtggaatac acttcatttc
     5581 cccagcggtc gaaaatcgcc atcctgaagt ctctcacgcc atatggaata cctaccggtc
     5641 catacaggtc atttccggaa ttggagctaa tattgataac attaggcaga taaagggtat
     5701 atcgtgggga aacatctaca atgatagcaa ggctgtccct acatccattt tcatgcgaca
     5761 cccattgtac catatgatag ataccggtat cccggtatgt atagattggg ttgcggtcag
     5821 tgctattatc tcctgttccg aattcatata accaagatga tgcatgacta ctttcatcat
     5881 gaattgttat ggtaggttcc agattggagg ggttggatgg ctcaaaagtg aatccggcca
     5941 caggtgattg cagtacagtt atggtattgt tgaatgtagc agatgttgta caaccaaata
     6001 catttgtcac cgtcacgtcg accgtataga tgcctctatt ttgataagta tgctcaggac
     6061 tgacgcctga tcccgactgg ccatcgccaa atttccagtc aatgatatac ttatctgtaa
     6121 tatagctatt gggcttttta aattgatagg taaacggctc acaacctatc acgccccccg
     6181 gatcaaacaa ttcatctctg ggtattggaa aaaacgcaac tctctctgcc acgctgtcaa
     6241 tacactgatt ctggtcggat actaccagaa tcatatcata gaagtcaggt tctaaggtgt
     6301 tgaattgtgc attgaatgaa ttggcaaata tccggccatt ggccttccat tcgctcgaaa
     6361 ttatggggcc cgcatcactg accgacatat tggtaaagtt gataacctta ccgtagcagg
     6421 tgtcataatc aaaggtaaaa tcggccctga tgtccggaaa aacattgacc ttaatggtag
     6481 tagaatctga gcagggcaag cctcgattga ggtacagcgt accgaaatac tgtcccagat
     6541 cttcggtatt caaacgataa tccttgcccg tgccattagt cacattatta ttgtggctga
     6601 aggaccattg taaatcaaaa atgtcattgg ttacctgacc ggcaaaggct acatccaaat
     6661 atttatcacc acatagcttg atatcaagat ctttaccaat cattccggta gctgagataa
     6721 cagcatctac cgtcttttca cacgaagtga cattaaattg aaaatcacgc cttatttccg
     6781 aaataagatt tccatttctg tattcccgaa gacaaatccc aactacaaat tgtccggata
     6841 taccaggagt acctttgagg aaacctgttg ccggatcaat cgataatggt gtaggccccc
     6901 caagcggatt ttggggactg taaggattgc ggaaggtcac ttcctcaaag ggtggtggac
     6961 aatcaggctg cggtgccacc acattacaac cattggtccc catatcgtca tttccacccc
     7021 cgacaaaagg ggcacaaagt gagtatacca gggagtctcc atcgacgtca gtagctgaat
     7081 gatcaaattc caaggggaag ttggcacaga tcgcgatcgg aggaaaatat ttgaattgag
     7141 ggctgctatt ttgaaggctt tgagcggacg gagttatctc caccatgaag gtagatccca
     7201 catctcccgg acgtacaatg ttagtgatgg tattattacg acagcaacgc tggtacacga
     7261 tatagtaact ggaagtactt acaggcaatt gaatcgttgt tgtataaatt ccctcttcaa
     7321 cgcatattcc gggtggaaca acgaggcatt tattagtaac aacaacatca agtttcttta
     7381 caatcggagt gccaaaattt atttctgcaa tgggcaggac cccatttcca cgatagatag
     7441 tacccaaagc cggaatttca cctatgagtc catcgaaatt ggccttattg gcctcaggct
     7501 tacaatcacg atacatcttt agggtgattc gataggtaat caaattcccg ctctgaccag
     7561 caaattcata atacatcaca cctccgatga tgtgcttggc ctgtataggt acagaaagca
     7621 gcagaaacac aaaaagcaga atatatgaat gcctcatttg attttaccca gataatataa
     7681 acgtctgaca cctatataat atatatggaa tcctacgaat gtgacaaaga taaataaaaa
     7741 aaattgccaa aggtattaaa tgggtttatt gcaggtgaca acaagcattg atttattatc
     7801 tttgtcatat ggattcctct caatacgaag cggatattac agcgtgtatc aagacactgc
     7861 aaacgggcgg cctaatttta tatccaacag ataccgtgtg gggtatcgga tgcgatgcgg
     7921 aaaatgaagt tgccgtccgg aaaataatca ggattaaaaa cagaccggat acaaagtcat
     7981 ttgttgtctt aagtcaaagc caatcaacag tccgtgaaat cgccgatccc atctctcaat
     8041 taatgctagc atttaaaaac aatgccttaa gaccggttac atttgtcctt tccaacgccc
     8101 gcaaggtagc tccgtctgta atcaatgagg acggcactat agcagtgcga atcccttctg
     8161 atgatttctg cagtaatctg ttgaaaaagt ttgggggacc catcgtttcc acttcagcca
     8221 atataagcgg acaacccaca ccgtcttttt tccaggaggt caatggaaaa atcagacaag
     8281 aggtggatta catcgtgcga tatcggcaag atgacatgac acctcgtcaa gggtccatca
     8341 tcattaaaga aagtccaaaa ggacaaattc ttatacttcg gtcttgaagc gtcctttact
     8401 ctttctgata tccactgcta ccaccttata ttccggacac atggcctcac tatcactctc
     8461 atcgctggtc agatcattca tattaatctc agggaaatgg aaggttgaag aaagaatacc
     8521 gggcctcaca tcttcagtta ctctggcacg cacatctatc ttaccccgag ggcttgacac
     8581 acataccatg tccccgtcag ctataaagtg tttttctgca tccaccggat tgatcatcag
     8641 ataatcttcc gtcagaataa tactgttcct ggttcttcgc gtcatcgtac ctgcattgta
     8701 gtgctccagg tttctgtttg tagtcaagat ataagggaac ttatctctgt gttccagcaa
     8761 ttcctccgtc tcacgaaaat cggcactttt aaatcgacct ttgcccagct tgaagatttc
     8821 ggtatgtaag atcttggtat ctgtaccatc cggcttgaca ggccattgct taccattgat
     8881 gccgaggtct tcccacttta tcccggcaaa gaaaggcaca atctgtgata tctcttccag
     8941 catggtagca gcattatagt ccggttgatt gcctcccatc ctattcatca attcgataac
     9001 aatctggcca tcaggtttgg ttcctgctaa tggctccacc actttattga cgcgttgaac
     9061 gcgacgttca ccattggtaa atgttccact tttttcaagg aaggaagagg ccggcaaaac
     9121 cacatgtgca tattttgcag tctcattcat gaaaatatcc tggatgatga caaggtccat
     9181 cttcttcagt gcctccacta caaaattgga attcggatcg gactggacga catcttcacc
     9241 catgatccaa aggcatttga gcttatcttc cattgatgct tcatacatct caggtatcat
     9301 caaaccacgg tgaatcggta gttttacctt atagaaatcc tcatacagct tgtggaattc
     9361 cggattggtg acatccatat aacctgctcc ctggtaaggc tgacatccca tatccgccgc
     9421 accctgaaca ttgttctgac ccctcagggg attgactcct acgccctttc gtccaatatt
     9481 tccggttatc atagccagat cggcaatcag cattacagca tatgtcccct gcgagtgttc
     9541 tgtcacaccc agtccgtgga agctcatggc attcggtgct gaagcatagg ttatagccgc
     9601 ttgtcttacc ttttccctct ccacgcctgt caccgcctcc atcttatcga tgtcaagctg
     9661 caagatgtgt tccttgaatt cttcatatcc ctctgtacgg gattcaatga actttttatc
     9721 ttccaggcct tcttttatga tgtaataaag catcatattg agtagggcga cgttggtacc
     9781 aggtctgagt tgaagatggt gcgtagcgta tctggctaaa acagtacgtc ttgggtcaat
     9841 gacaatactc ggattgtcct tcatcatgaa ctgcttcaat ttagcccccg ttaccggatg
     9901 tgcactggtc ggattcgccc caataaccat gatgcaatcc gtatgtttca gatcttcaat
     9961 actattagtc gccgctccgg tcccgaaagt ccgctgcatt ccaattgctg taggcgagtg
    10021 gcacaccctg gcgcaattgt cgatgttgtt ggtacctata atgaccctta ggaatttttg
    10081 catcagataa ttctcctcat tggtacatcg tgacgaagat ataccagcga tagcatcagg
    10141 accgtaatgt gtcttaattg cagtcatttt ctcgacgata taatcatagg cttcgtccca
    10201 ggtaaccggt tccaactcac cgttgcgtcg tatcatcggc gttgtcagcc tatcaggatg
    10261 attgtagaat gaaaaggcat atcgcccttt gagacaggta tggccggcat ttacctcggc
    10321 atcatatgga gctcttatag acttaacttt accctcgacc gacgaaactt ccaaattaca
    10381 gccaacacca cagtatgtac atacagttcg agtaatatca gtacctgcat atgccctgga
    10441 atcgaacaca tcgttaatcg ctccagtagg acaagcttga gcgcaggcgc cgcaactgac
    10501 acaatcgctt tcaaagtaac ttacatcgga gcctttgatt actttgctct caaaagccct
    10561 gcctgccatg ctgagcacca tttctccctg cacctcatca caggcccgga tacacctgaa
    10621 gcaattgata caaagtgaca tatcggacac catgtaagga tgagacaaat ccttttcgta
    10681 atggaaatga ttctcgccat cagggtatct tacttcgctt attccgactc gtgctgcgac
    10741 atcctgcaat tcacaattgt tgttggatgc acaaccggaa catgtcaaag gatggtcagt
    10801 caatactaac tctacaatat tcttccttaa cttctgaatt cgatcattat tggtttgaat
    10861 ataggtattg gccatgaccg gggtatgaca actggcttgc gctttggagg atccattggc
    10921 cgtaagcgcc acatcaacgg tacaaaccct gcacgacccg aaaggttcca gattaggagc
    10981 gtcgcaaagt gtaggtacca gattatatcc atgattacga cggataaaag agagtatggt
    11041 ctcaccgtca cgcatattat atggaaggcc atcaatatag gctatctttt catcggatcc
    11101 tgtatcgcct ttaagtccag ccggaatttc cttttctgtg attgtactgc ttgtgatttt
    11161 catatcaagt ggtttgaaaa ctgtttaaaa tatttttcca atttcaaaaa aatcatcatt
    11221 ttatcctgca aattcttcct taaaataagt ttttaattca tcgtcaaaat acatcagtgc
    11281 attgcgcatt ggtaagggca caccgccacc cagtgcacaa agagaaccta tttccatggt
    11341 ttcgatcaag tcacggaata gttgccgatc aattttgtaa ttatgcttta gcgctttttc
    11401 aatcaattct gcacctctct tactgcccaa acggcaagga aagcatttac cgcaactttc
    11461 atccgcagta aattcaaaca aatgttgcaa atattccatt atggagaaat ccaggggtag
    11521 acagactatg gatgcatgtc caag
//
LOCUS       DAOQMC010000051        11477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2184_length_11477_cov_5.874803, whole genome shotgun sequence.
ACCESSION   DAOQMC010000051 DAOQMC010000000
VERSION     DAOQMC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2184_length_11477_cov_5.874803"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKV31_03450"
     CDS             complement(<1..344)
                     /locus_tag="PKV31_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNB91941.1"
                     /translation="MALYELVMPKMGESIMEATILNWAKNVGDHIDIDETILEIATDK
                     VDSEIPSPVEGTLSKILFNVNDVVPIGTVIATIETGAPVGSAVTDESASSEEHSASKV
                     PFVPEEGTKQSIT"
     gene            complement(1041..2519)
                     /locus_tag="PKV31_03455"
     CDS             complement(1041..2519)
                     /locus_tag="PKV31_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91942.1"
                     /translation="MKRLIFGILMYGFVLANSVSAQSSLDVLRLSGQNLLGTARAAGT
                     NTTMSGVGADFTTLSSNPAGIAQYRLSEIMVTPSVVDNRTKSRLADGSERPETSDSKT
                     KFRLPNLGLVIAKQNSDRMSYAFGFGINGYVDNSQNSLFRGRSKGSIVERFAALANGL
                     SLEQLDNYEAGLAWDTEALVQQRPDGSYSFDYEDYKDQELNKYQSISASNRYNDIAIS
                     GGINLNNKILVGASIGIPTFSYNERKIYQEQDDIGAVPYFDQLQFREYKSASGVGVNG
                     KLGIIFKPINNILIGAAIHTPSFISVTDNFSTDLRYVFTFNSEKKNNYKESPEGTIDY
                     KVTTPMRLLGSIGGIIGKFGFVNAEVEYQDYSGSKIRIDSDNLYDKQVEEQINGEIKA
                     NYTSTVKVNLGIEVAAIEHFRIRAGVGFAQTPFVGQRNFYPNFGLGLGYRSDVFFLDL
                     GFRQWRSENGYQPYYITQASQTLVNNKLTNSAVLLTIGFKLR"
     gene            complement(2822..3880)
                     /locus_tag="PKV31_03460"
     CDS             complement(2822..3880)
                     /locus_tag="PKV31_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91943.1"
                     /translation="MFLMFGTIAFAQFDDVYFNPKKDVLPGSYTSSSNSKATDSDYGY
                     SYNSGNTGAINGGYEGNNTINSDEYDDQSYEYYEDYTSRINRFHRYNVSGFGYYDPCY
                     TGGFGYNSWSFRPSVTFMYDPFFNYNRYYNNSFWGSPYYGWYNDPFFDYGYPYYGYGY
                     GYGYPYYGYGYPYYGYSPYYGGHNGWYGNGWYGDSDKNKVSRVYGARYGGAVVGGSDG
                     YRDRSKMTNGTINPNDSRSRLNNGSKPELEVRNTNTNRGRVDGSSSRPDRSNKTNNPD
                     RGTRINTERPRSTPQRETIPTRESMPSRTYEAPTRSRSTETYSPSRSGNSSGSYNSNS
                     NSSGTERGTSGSSSRSRG"
     gene            4551..6017
                     /gene="proS"
                     /locus_tag="PKV31_03465"
     CDS             4551..6017
                     /gene="proS"
                     /locus_tag="PKV31_03465"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNB91944.1"
                     /translation="MSKEITPRDENYSAWYNDIVIKAGLADHSAVKGCMVIKPYGYAI
                     WEKMKDQLDAMFKDTGHVNAYFPLLIPKSFLSKEAEHVEGFAKECAVVTHHRLMNDPN
                     GKGVVVDPSAKLEEELIIRPTSETIIWNTYRDWIKSYRDLPLLINQWANVMRWEMRTR
                     LFLRTAEFLWQEGHTAHATADEAIEEARTMLNVYADFAENYMAMPIIKGVKSANERFA
                     GALDTYTIEALMQDGKALQAGTSHFLGQNFARAFDVKFLTDKNTEELVWATSWGVSTR
                     LIGGLIMTHSDDLGLVLPPRLAPIHVVIVPIPKANDDIDKIALDILAQLKLKGITAKY
                     DNDEKRRPGFKFAEYEMKGVPIRIGIGARDLANGEVEMARRDTLEKSQVKLEGLVDTI
                     VNTLDLIQNNLLERSRQYRDQHITDVNSFDEFKDVLTNKGGFVRAFWDGTTETEEKIK
                     ELTKATIRCIPLDSKEEEGICVYSGKKATQKALFAIAY"
     gene            6030..6380
                     /locus_tag="PKV31_03470"
     CDS             6030..6380
                     /locus_tag="PKV31_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNB91945.1"
                     /translation="MSFEISGKLVKKYDVESKTATFQTREFVIVTEETYPQFIKFQLS
                     QERVNLIDAYNENDVIKVHFDLRGREWQGKYFTNLNAWKIEGVTASNSASGHPGNDVP
                     FEINDEPFENDLPF"
     gene            6440..6958
                     /locus_tag="PKV31_03475"
     CDS             6440..6958
                     /locus_tag="PKV31_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91946.1"
                     /translation="MALQAQTDSTELNISGLSIAREDDFTDWDFYGNTDDPIGYLRLR
                     WIFQKDWSEWDYRIGEETGSIKQRTKSDPNIWELRSGNVILTLKTIYTGDFNQWRITD
                     DSQTLTFSTLYENVYDGWKTSSKAGQFEIYTQWEMDPRDWIVYDKLKPEISNPFKIAM
                     IFLASYYSSPKY"
     gene            complement(7655..10687)
                     /locus_tag="PKV31_03480"
     CDS             complement(7655..10687)
                     /locus_tag="PKV31_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HNB91947.1"
                     /translation="MNTFTLSSMIGFWRKTFIVLIAFATWTGVPNESNAHNVPGILKE
                     NAEKIDISQWVICPPSWINVSCSQLDPGLNYGNPTIAYGCGCGAVIYGPYITDNRVGC
                     GSGTIIKTWNIWTMYGDYTCVQTINVTGGYNGYPVIQWPPDYTVTGCDASTAPESLPP
                     PYNKPTWWAPECSQLMYTWYDEVFYTYNVQGICKKILRHWKVIDWCVYDVDHPWYGGW
                     WACTQLIMVKDQYPPSINCPGDVVANAGPNCSGSYVHIPPAYSTDPCGNVNITNNSPY
                     SVYHGADASGYYPPGTTKVTFCARDACGNSTYCTINVTVTDKKKPSPVVYYGLSTSLM
                     CVNPQPMIEIQAKWFDAGSFDNCTPKSRLKFDVYPKVFTCADRGYNDVTVTVTDEAGN
                     KETVKTFIIVDDNTGCCGPDTTNPPVLTCPGDVVIDAQSADCSGAFVDLAPATATSDC
                     NGAVNITNNSPYATSHGANASGVYPIGTTVVTFTAVDYCNKKTTCKATIRVRDGKKPS
                     PVVFYGLAISLAEDTINGGGTITLVPEWFDAGSFDNCTSNNNLKFDVHPKTFNCDSLG
                     ERNVRITVTDESGNSEYVDTYIVIQDPSGVCVTDFTANVAGLIKTEDGEKVSGVDMFA
                     ATQDTMYQKRVDGDYVLLGLKGGKEYEIAPKKADDYRNGVSTRDLIILKNHILGKSLM
                     QSPYKLIAADVNNDKIINTADLILLRKLVLGTIDTLPGSFSWKFIDGNYIFDPFKPAL
                     TQNYPTYVKIQKLKDDKDGVSFIGTKMGDLNGSVLASMTSGSSVENRNNGNIKLYALA
                     SEKQRDVRVDFTPEMTVNADGIQMEMSFDPTILRFKGINPGMLTGFNSDVNMNLSGIA
                     NGIIKLSWNANNAVTVDPSAPMFSILFERLTESGEVKLKLNNTGFNAELYDQFENTFN
                     IELINEKVESNDGFALFQNRPNPLQSFTTIGFNLPEASDIELSIFNLNGDLVRQIKGH
                     YSKGTNNINVEWNNTRGVFYYTLKAGKNAATKKMIVVE"
BASE COUNT         3543 a         2440 c         2080 g         3414 t
ORIGIN      
        1 gtaatcgact gcttcgtacc ttcctccggt acaaaaggta ccttagaagc agaatgctct
       61 tctgaagagg cactctcatc agtaacggca ctaccgactg gtgcccctgt ttctatagtt
      121 gctatgactg taccgatggg aactacatca ttgacattga acaatatttt tgacaatgtt
      181 ccttcaacgg gagaaggtat ctcgctatct accttgtcgg ttgcaatttc aagaatagtc
      241 tcatcaatat caatatgatc ccctacattc ttagcccaat tgaggatggt tgcctccatg
      301 atactctctc ccattttagg cataaccaat tcgtacaaag ccatagttct gttttaattt
      361 cgaaaattca tgcaaaaata aggttttaac gcctaatatt tttacatttg aatccctcat
      421 tattacgaat atgtttattc ttatatatac aattttgggt tataatccat tacaatcaaa
      481 cccaccctgt tttctatcat aaataataat ggtaggaacc tcaagtatat tattcgatat
      541 ttcatttgac attattttat aagactccaa ttagataggg ccggggcgca atacagcgat
      601 tggaagatgg aggcttggca atttcaattg taatacttga aagtgcggat tattgacatt
      661 caaatgattt agttggggtg cccatttgaa gtgatcgggc ttaaaaaatg ttacatgtgc
      721 cttagaaacg gctttctggc ctttatactc gaaaatcgct gtagcggatc taccgattaa
      781 caaattcaaa ctactatcaa tactttaggt ataacatcaa tttcggctga ttaggcctat
      841 aaaactctta caactggtaa aagttagacg gccaagaaat cggcagaaaa ctcatcctac
      901 aaccaaatat gaataaatta ctataaaaca ggaataaata aagagaattt aaacataagg
      961 catttcacgg gtattgaatc acccaaagtc cttatatcac agccatatcg ggctccttag
     1021 agatcctgtg aatgcattgt tcaacgcaat ttgaatccta tagtcaacaa tactgcacta
     1081 ttggtcaatt tattattgac cagggtttgg ctggcctggg ttatatagta gggttggtag
     1141 ccattttcag acctccattg tctgaagcca agatccagaa agaatacatc actccgatat
     1201 cccaatccaa ggccaaaatt tggatagaaa ttacgctgac ccacaaaggg agtttgcgca
     1261 aaaccaacac cggcacgaat cctgaagtgc tcaatagcag ccacctcaat accaagattg
     1321 accttaactg tagaggtata atttgctttt atctccccat tgatctgctc ttcaacttgc
     1381 ttatcatata aattatccga atcgatcctg atcttacttc cagaataatc ctgatactcc
     1441 acttcagcat tgacaaaacc aaacttcccg ataataccac caatacttcc cagtaaccgc
     1501 atcggagtgg taactttata gtcaatggtc ccttcagggg attctttata attgttcttt
     1561 ttttcactat tgaaggtaaa tacatacctt aaatcagtgc tgaaattgtc tgttactgag
     1621 ataaagcttg gtgtatgtat ggccgctccg atgagaatat tattaatagg cttgaaaatt
     1681 atacccaatt tgccatttac cccaacacca gatgctgatt tgtactccct gaattgtaac
     1741 tgatcaaaat atggtacagc ccctatatca tcttgctcct gatatatctt cctctcattg
     1801 taactaaaag tcggtattcc tatcgatgct cccaccagaa ttttattatt cagattgata
     1861 ccgccggata ttgctatatc attataccgg ttgcttgcac taatggattg gtacttgttc
     1921 aactcctggt ctttatagtc ttcatagtca aacgaatacg agccgtcagg cctttgttgt
     1981 acaagagctt ctgtatccca ggccagccct gcctcataat tatccaactg ttcaagactc
     2041 agaccatttg ccaatgcggc aaaccgctcc actatcgacc ccttgctcct ccctctaaat
     2101 agcgaatttt ggctattgtc aacgtatcca ttgatgccga atccaaaagc atagctcatc
     2161 ctgtcgctat tttgcttggc tataaccaat cccaaattgg gcaaacgaaa ttttgtttta
     2221 ctgtcacttg tttccggacg ttcgcttcca tcggccaatc tcgacttcgt acggttatcc
     2281 actacagaag gagttaccat gatttcagac aatctgtact gagcaattcc ggccggattg
     2341 gacgataacg tggtaaagtc cgctcccaca ccgctcatgg ttgtattagt cccggctgca
     2401 cgggcggttc caagaagatt ttgtccggac aagcgaagga catcaagcga tgactgtgct
     2461 gatacggaat ttgctaaaac gaaaccatac atcaatattc caaaaattaa tcttttcatg
     2521 gctttactaa ataatattgt taaactattt gttgaggcaa tagtttattg gatcaaaaaa
     2581 ataccaggtt gcggtttgtc attgcgcctc attcaataga cacgcctccc tgattcatca
     2641 tcttcattta ttgcctgatg gatgattatt cgctttccat caaatgttta aacaccattt
     2701 atcatacccg actgtattaa tgaggacaga atcagtccgg tatagaataa aataaggtgg
     2761 caaaatatga tcgtaccacc ttatttgagt tataaaaata aaattgacat tatcaatact
     2821 atcaacccct tgacctggaa gacgaaccag atgtacctct ttcagttcca cttgaattgc
     2881 tgttggaatt ataagaacct gaggagttgc cggatcttga tggtgaatag gtttcagtcg
     2941 atcttgatct ggtaggcgcc tcataggttc tcgaaggcat tgattcacgt gtaggtatgg
     3001 tctctctctg tggtgttgat ctcggacgtt ctgtatttat ccttgtcccc cgatccggat
     3061 tatttgtttt gtttgacctg tctggcctgc ttgaacttcc atccacacgg ccccggtttg
     3121 tatttgtatt cctcacttca agttcaggtt tggagccatt gttaagtcgg ctacgactat
     3181 catttgggtt gattgtgccg tttgtcattt ttgacctatc ccgatatccg tctgagccac
     3241 ccactacagc accaccatat cttgctccat aaaccctgct aactttattc ttatcggagt
     3301 ctccgtacca accatttccg taccagccat tatgcccacc ataataggga gagtaaccat
     3361 aataaggata accataccca tagtagggat atccgtagcc ataaccataa ccatagtaag
     3421 gataaccata atcgaaaaac ggatcattat accacccata gtatgggctt ccccaaaaac
     3481 tattgttata atatctgtta tagttgaaga acggatcgta catgaaggtt acacttggcc
     3541 tgaatgacca actgttatac ccaaatcctc ccgtgtaaca agggtcgtaa tagccaaacc
     3601 cactgacatt atatctgtgg aatctgttga ttcttgatgt atagtcttca taatattcat
     3661 aactctggtc atcatattca tcggaattaa tggtattatt gccttcataa ccaccattga
     3721 ttgcccccgt attgccggaa ttataactat atccataatc agaatctgtt gccttactat
     3781 ttgaagatga ggtataagat ccgggcaaga catccttttt gggattgaaa tatacgtcat
     3841 caaactgggc aaaagctata gtcccaaaca ttaggaacat tatcatagga aataagaatc
     3901 gcaaagcttt cattttaatg aatttattat taatcacaac ttgtttatca attcaacagc
     3961 taaagtatta cttttatccc caagaattag ttaaattatt gataatattt attttcagtt
     4021 agttaaaact ttatttatcc aaaatcattt tcacgaaaat aaccactggt aaatgatttg
     4081 caactattcg ccgctccggt tctcaagtta ccgattttat tgctaatccc ccgcaatggc
     4141 tctatatact attgattccc aagtgaggca gtttgtcaga ttgtcatacg ccttgtcctt
     4201 tgtttatgta ttaatttctt tgttgcacaa ttcagaaaac acacgggcct cgcaattttg
     4261 tcaaaatatc acggttaaag tgttttatag ccgaaacaaa aagatgatat tatacgataa
     4321 ataattacaa agattttaca cgctgtgcaa ttcaatcagg acaaacttat taatatattt
     4381 attcgctttt atcaactaaa atttaaagaa aatacctaat ttaattttct tttaaggaat
     4441 taattaaatt tgcgcctgaa tacggaagga ttatttatct gcaatactgg aattaagaca
     4501 ttttcagtga agtaaagcga agtttcaatt tcaacatttt aaacaggaaa atgagtaaag
     4561 aaataactcc aagagatgaa aactactccg catggtacaa tgatatcgtg atcaaagccg
     4621 gcctggccga tcattctgcg gtcaagggat gtatggttat caaaccctat gggtatgcca
     4681 tctgggaaaa aatgaaggat caactggatg ccatgtttaa ggataccggt cacgtcaatg
     4741 cctactttcc tctcctaatc ccaaaaagtt tcttgagcaa ggaagcagaa catgtggaag
     4801 gttttgccaa ggaatgcgca gtggtcactc atcacaggct tatgaatgat ccgaatggaa
     4861 aaggggtagt tgtagatcct tctgccaagc ttgaagagga gcttatcatc aggcccactt
     4921 ctgagaccat catttggaat acctaccgag actggatcaa gtcctatcgt gacctgccat
     4981 tacttatcaa tcaatgggca aatgtcatgc gatgggaaat gcggacaaga ctttttctca
     5041 ggactgcgga atttctgtgg caagaaggac atacagcaca tgcaactgca gatgaagcaa
     5101 ttgaagaagc caggaccatg ctcaatgttt atgcggactt tgctgaaaat tacatggcaa
     5161 tgccaattat caaaggtgta aaatcagcca atgaacgatt tgccggcgca ctagacacat
     5221 acaccattga agctttgatg caagatggta aggcactgca agcgggcacc tcacatttcc
     5281 tgggacaaaa ttttgccaga gcttttgatg ttaagttcct tactgataaa aataccgaag
     5341 agttggtatg ggcgacttca tggggtgtat ccaccaggtt gataggtggg ttgattatga
     5401 cccattcgga cgacttggga ttggttctgc ctccacgttt agctccgatt catgttgtaa
     5461 tagttccaat acctaaggca aatgatgaca tcgacaaaat tgctctcgat attcttgctc
     5521 aactcaaact aaaaggaatt actgccaaat acgacaacga tgaaaaaaga agacccggat
     5581 tcaaatttgc cgagtatgaa atgaaaggcg tgccgatcag aatcggtatc ggtgccagag
     5641 atctggccaa cggcgaggtg gaaatggcca gaagggatac tttggaaaaa tcacaggtca
     5701 aactggaagg attggtcgac acgatcgtaa atactttgga tttgattcaa aacaatcttt
     5761 tagagaggtc cagacaatat cgagatcagc acattacaga tgtaaatagt tttgatgaat
     5821 tcaaggatgt tttgaccaac aagggtggtt ttgtcagggc attctgggac ggtactaccg
     5881 agaccgaaga gaaaatcaag gaactaacca aggctaccat tcgttgtatt cctttggact
     5941 ctaaggaaga agaaggaatt tgtgtctatt caggcaagaa agcaactcag aaagccctat
     6001 ttgcaatcgc ttattaaatc caaaaaaata tgagttttga aatttccggt aaactggtaa
     6061 aaaaatatga tgtcgaatct aaaactgcca catttcagac acgtgagttt gtgattgtca
     6121 cagaggaaac ctacccccaa ttcatcaagt tccagttgtc acaagaaagg gtcaacctga
     6181 ttgatgcata caacgaaaac gatgtaatca aggtacactt cgacttgcga ggacgggaat
     6241 ggcagggtaa atactttacc aatctgaacg cctggaagat tgaaggtgtg acagcaagca
     6301 acagcgcctc cggacatccg ggaaatgatg tcccgtttga gattaatgat gagccgtttg
     6361 aaaacgactt gccgttttaa agataacttc caacatcacg atgcgctata acctgttgct
     6421 cataatggtt tttatcggaa tggcattaca agcacagacc gattcgacag aattgaacat
     6481 ttccggattg agtattgcca gagaggatga cttcaccgat tgggatttct atggcaatac
     6541 agacgatcca ataggatacc ttcggcttcg atggatattt cagaaagatt ggtccgaatg
     6601 ggattaccgc attggtgaag aaacgggctc aatcaagcaa cggactaaat cagatcccaa
     6661 tatatgggag ttgcgttcag gcaatgtcat tctcacctta aaaaccattt ataccggaga
     6721 ctttaatcaa tggcgaatca cagatgattc acaaacccta acattttcta ccttgtatga
     6781 aaatgtatat gatggttgga agacaagtag taaagctggc caattcgaaa tatacaccca
     6841 atgggagatg gatcccaggg attggattgt ttacgacaaa ttaaaacccg aaataagcaa
     6901 cccctttaag atcgctatga tttttctggc aagttactat tccagcccca aatattgacc
     6961 gactccacgg ttcaagctgc cggtaagcat gattgccaga aggtgaatac tatgtaattg
     7021 cccatcttgt gaatgtcgtt cccttgtacc aaacattcct aaaccaaaag tcaataaatt
     7081 aattttgcac gtttgtggca gatgaatgtt gtcctaagta ccaatgattg caacagtaat
     7141 tcttggcact ttcattttat tcaaacagca tatagatttc acaatgggaa atttgtaaca
     7201 aatgagtcat tctccgttcc attctttgac cacatttcct acatatctcc aaagaaacat
     7261 ccatccttcc aactacagcc aaaacatatt tgctattgtg tctatagatt ttcaagtgtt
     7321 gaaaagataa tgatgttaaa taccgaaggt acattcgtta tagaccaatt tcattgatct
     7381 atacgactgt aacctcggca tcataccaaa aatttataga ccattttata aatcatcttg
     7441 gtataagata actgaagatc gggaaaaatc tacaagacag ataattccat ttgccaatca
     7501 aaaccaagac agaagcgatc cggatttcga cccttcattt aatccatggc agacacagac
     7561 cggtcaatca atctttacta ccaatctggg tttgtcacaa aaaaaagagg cttgccataa
     7621 tgccaagcct cttttttaat atttaatcag caacttattc tacaacaatc atcttctttg
     7681 ttgcggcatt cttacctgcc tttagtgtat aatagaatac accgcgtgta ttgttccatt
     7741 caacattgat attgttggtt cctttactat aatggccttt gatttgacga accaaatctc
     7801 cgttaagatt gaagatactc aactcgatgt cggatgcttc cggcaagtta aatccaatgg
     7861 ttgtaaagga ttgcaatgga ttaggtctgt tttggaacaa tgcaaacccg tcattgcttt
     7921 ctaccttctc attgatcaat tcaatattga aagtattttc aaactgatca tacaactcag
     7981 cattaaaacc ggtattgttt agcttcagtt tgacctcccc tgattctgtc aatctttcga
     8041 ataggataga gaacatcgga gcactaggat caacagtcac tgcattattc gcattccaac
     8101 ttaactttat aatgccgtta gcaattcctg aaagattcat atttacatca gaattgaatc
     8161 cagtgagcat tccaggattg attcccttaa atctgaggat tgtaggatca aaggacattt
     8221 ccatctggat tccatcagca ttgactgtca tctcaggtgt aaagtcaact ctgacatctc
     8281 tttgcttttc ggatgccaat gcgtataatt tgatattacc gttattcctg ttttcaacgc
     8341 tgctaccgga agtcatactt gcaagtacag agccattcaa atcacccatc ttggttccaa
     8401 taaacgaaac tccatcctta tcatccttaa gtttctgaat cttgacatat gtaggatagt
     8461 tctgagtaag tgcaggcttg aaaggatcga aaatataatt accatcgata aatttccaac
     8521 tgaaactacc aggaagagta tctattgttc ctaaaaccaa tttacgtaac aaaatcaagt
     8581 ctgctgtatt gatgatcttg tcattgttga catcagcagc aatcaactta tatggacttt
     8641 gcatgagtga cttacccaaa atatgatttt tcagaataat caaatcacgg gtagacactc
     8701 cgtttctata gtcgtcggct ttcttcggag cgatttcata ctcttttccg ccctttagac
     8761 ccagcaatac ataatctccg tccactctct tttggtacat tgtgtcctga gtagcagcga
     8821 acatatccac accggaaact ttctctccat cctccgtttt gattaaaccg gcaacattag
     8881 ctgtaaaatc ggtcacacaa acccctgaag gatcctgaat tacaatatag gtatcaacat
     8941 actcggaatt accggattcg tccgttaccg tgattctgac atttctttca cccagactat
     9001 cacaattaaa tgtcttagga tgaacgtcga atttcaggtt attatttgat gtacaattat
     9061 cgaatgaccc ggcatcaaac cattcaggga caagagtaat tgtaccccca ccattaatag
     9121 tatcttcggc taaagaaata gccaatccgt aaaataccac cggagaaggt ttcttaccat
     9181 ctctgaccct aatagttgcc ttgcaggttg tcttcttatt acaataatca acagcggtga
     9241 aagtcacgac agtagttcca attggatata caccacttgc attcgctcca tgagaagtgg
     9301 cataaggaga attgttggta atatttacag caccgttaca gtcactggtc gctgtggccg
     9361 gagccaaatc aacaaatgcg cctgaacaat ctgctgactg cgcatctatt acaacatctc
     9421 ccgggcaagt taataccggt ggattggttg tatccggtcc acaacagcct gtattgtcat
     9481 ctacaatgat gaaagttttc acagtttctt tattacctgc ctcatcggtt acagtgacag
     9541 tgacatcgtt atatcctcta tctgcacaag tgaatacctt tggataaaca tcgaatttta
     9601 atcttgattt tggagtacag ttgtcaaatg aacctgcatc aaaccactta gcctgaattt
     9661 caatcattgg ttgcgggttg acacacatca atgaagtgct caaaccataa taaaccacag
     9721 gacttggctt cttcttgtca gtcacagtaa cattaatggt acaataggtc gaattgccac
     9781 atgcatccct ggcacagaag gtaacctttg tagtacccgg aggatagtat ccactggcgt
     9841 cagcaccatg ataaacacta taaggggaat tatttgtaat attcacattg ccacaagggt
     9901 ctgttgaata tgccggagga atatgtacat atgaacctga acaattgggt ccggcattgg
     9961 cgacaacatc accgggacaa ttgatagatg gaggatattg atccttaacc ataattaatt
    10021 gggtgcatgc ccaccaacct ccataccatg gatggtccac atcataaaca caccagtcaa
    10081 taaccttcca atgacgtaga atctttttac atataccttg aacattatag gtatagaata
    10141 cctcatcata ccatgtgtac atcaattggg agcattcagg tgcccaccat gttggtttat
    10201 tatagggtgg tggaagtgac tcgggggcag tggaggcatc acatccggtg acagtataat
    10261 ccggtggcca ctgaatgacc ggatacccat tgtatccccc tgtaacattg attgtctgta
    10321 cgcatgtgta atcaccatac atcgtccaaa tattccaggt tttaataatg gttcctgaac
    10381 cgcaacccac tctgttgtca gttatatatg gaccatatat aacagctcca catccacacc
    10441 catatgcaat agtaggatta ccgtaattca atcccggatc caactggcta cagctaacgt
    10501 taatccagga aggcggacaa ataacccact gacttatgtc gattttttca gcattctctt
    10561 taagaatacc tggcacatta tgcgcattac tttcgttagg aactcccgtc catgtagcaa
    10621 aggcaattaa aacaataaat gtttttcgcc aaaaaccaat catcgaagaa agtgtaaaag
    10681 tgttcatgat catattttgt aatttagatt tcgttattga ttctgaatta gttactaaac
    10741 cagttattca ttattttgag agtggctgcc ggatgaacaa aaagaatttc atactaacct
    10801 gagcacgaag aaacttctga tcgatttcct tccgtccaag tatcttcaat taaatcatca
    10861 gcttaataag caatatagtc ttgaatttcc tgtctatttc tgttggacta aaccttattg
    10921 tccaataata gattacaaat atatttttat tggtgcaatt aacaaatttt tcaaactaat
    10981 aatttttttt taaatgtgtt aatacaagat atagaaacag taaaaaatta atcaccaatc
    11041 ctcaattttc tgtcaaaaca caaagtttgc cattccaaaa tgcagtccac gaggcacatg
    11101 atagcctcac aaatctctta tatattgaaa aatattcaat attaagtgcc ccaatttact
    11161 gtaataagga agccccaatt gccatcacca caatccaatg gattataaaa aatctataca
    11221 ggaggaatac ctgatccctg agccaaaatg actttgacca actaaataca tcacaaaacc
    11281 ctaatcacgt tcttcagagt ggctttctag ttggtgtgcc tataaagcaa aaatagtttt
    11341 tgttcttaca caggcaaaaa aaaagctccc gcttcgaatg aagcgggagc cgtcccaaaa
    11401 accgatttta aattatgtta tctcaacttt cctttaaaaa cggtctcccg tatttaatgg
    11461 gcttacctga ttactcc
//
LOCUS       DAOQMC010000052        11450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2191_length_11450_cov_4.957525, whole genome shotgun sequence.
ACCESSION   DAOQMC010000052 DAOQMC010000000
VERSION     DAOQMC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_11450_cov_4.957525"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="rsmA"
                     /locus_tag="PKV31_03485"
     CDS             complement(<1..761)
                     /gene="rsmA"
                     /locus_tag="PKV31_03485"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNB91948.1"
                     /translation="MSFSKFPSPKKSMGQHFLHRQDIAEAIVHACDMTSTQGRVLEIG
                     PGPGVLTEILMGRNLDLKCVELDRDMVILLNKKFPALSDHIIQNDVLRLDFNSVFDGK
                     SFNIIGNFPYNISSQIVFQIIHYKELIPQMVGMFQKEMAERIIAPPGGKDYGVISVLT
                     QAWYHGRKIIHVPPSAFNPPPKVDSLVIELLRRNEDIGIKDIKAFTTVVKQSFAMRRK
                     MLRNNLKTLFADESNLADSFFNQRAENLSVSDYVQ"
     gene            911..2158
                     /locus_tag="PKV31_03490"
     CDS             911..2158
                     /locus_tag="PKV31_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB91949.1"
                     /translation="MHISGRFIIPFLLFVLIDIYAFQAFRGSLRSLNPDFRRFLYYGY
                     WILSAIAYLAATAMITGILTERQHFLMVYGRTLLMILFTVKALSIPFLLIDDIRRGTL
                     AVIQYFSPANSYDPSRSRFLSTLSLAMGAIPFALLSYGAVRNPYRYKVRRHKVFIEDL
                     HPDLEGFKIVQFSDVHSGSFVFKEPIAHGIDLINKENGDLIVFTGDIVNTLAEEMDGM
                     YEIFSRVGGPGNTVYSILGNHDYGNYHQWNSKAEEQKNFEEVKNIHHKLGWDLLLNEH
                     RVLTHKAARIGLVGVENFSALPQFPKHGDLKRAVDGMPGVDVRILLSHDPSHWDFEVI
                     KEHKDIDLTLSGHTHGFQFGVEIPGWFRWSPSRFVYKQWAGLYQKGRQYLYVNRGFGF
                     LGYAGRVGILPEITTLVLTGKKE"
     gene            2284..4074
                     /locus_tag="PKV31_03495"
     CDS             2284..4074
                     /locus_tag="PKV31_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB91950.1"
                     /translation="MKAWIAFLLYFLLLGNCHILKAQAINDDCFSATELTNVRSWCSS
                     NGFFNNFSATPSGYGPATCFSSSGHDVWFSFTAIATDITILVKPGSLQKPEIALYAGI
                     CQGTIGELNCKSNLVTGGALELYKGGLFPGTVYLIRVQGEDASEGTFDLCLDNYFPPV
                     KPGSDIITASVLCDKSSFIVQNVTGSGSVPNEFKDAPCLADGFTNSETNSTWFKWTCK
                     DAGSLTFVLTPLNAPDDLDFALYELNGDLNTTNRTLLRCEAASCKGQTGLKEGATDIS
                     EPPNCDKGQDNWLAPVQLEAGKNYLLGINNFTIGGNGFRIEFGGTSTFEGPVADFEIL
                     SDTVFCREEQIGIKDLSHNSTGTIEKIQWIFGQDATPSIDSIQGDKTISYLKRGQKFI
                     VQTVTSDKGCVVSTIKPVEIQCCGPLHEMNLYSDTTITLGDSVDLHAEAFLEGNQIFY
                     FWEPPGLIQCQGCPANNIKTFHDQVIVVKATDQYNCEAKDSIQIRVDVKRPIFFPNVF
                     SPNYDGINDRFTGYGNFVVRTIDYLRIFNRWGALVWEGRNIPANDEKYGWDGTFKGRE
                     VNPDVYVYIAKVTFIDDQSFIYEGSITVVR"
     gene            4028..5878
                     /locus_tag="PKV31_03500"
     CDS             4028..5878
                     /locus_tag="PKV31_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB91951.1"
                     /translation="MINHLFTKVLLPWSGRLLRIVSVLALFLWPANLVFCQTINDECK
                     KAIFIPNVKEYCSGDTTFSFLGSTPSNNGFSLCQTDNGNDVWFSFHVTATDVVIKILC
                     KEANDLHASLYSGDCGSLNEETCGRREAGQNFLQLYKGGLVPGTRLYLRVDSENGTNL
                     KFGLCINNFFSPALDGSDCKTANLICSKESFTVRNVQGFGEVNELGNAPCFSHANESN
                     SIWFRWICRDPGTFTFKLIPLKEADDIDFVVYKVRGDAFGCDLELVRCMATGISPVNC
                     TNYPNCCGSTGLRDGETDESEGAGCGPDRNNFLKPLDMESGEVYAILINNYTSQNEGF
                     DFNMGGTATLKSLQADIDIIKPDALCREESLEVKDKIINDLGTIKSLLWTFTPDASIS
                     TSQGAGPVSVHFDRSGYKTISLIVENDAGCKAYAQDTVFVFCCGGTLRADILGNTFVD
                     YGEMAEFGVQFVLEGEDVKYQWTPAGQVNCDTCSLITLDPVYEDFPLWVTVSDENGCK
                     AENRVFIKTGKPEVFAPNIFSPNHDGINDRFMLSFAEGIHRIRSLRIYNRWGGLVYEG
                     RNMDKSDADGGWDGRFKDRDAAPGTYVYYAELETRGGKTMVIKGGLTLIR"
     gene            5918..6484
                     /gene="lptC"
                     /locus_tag="PKV31_03505"
     CDS             5918..6484
                     /gene="lptC"
                     /locus_tag="PKV31_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNB91952.1"
                     /translation="MNISFRTFLGLCLLALSFQACENDLAEVRRVTGVSVSGSYEEGV
                     DGEILYSDSARLKVVIKFDTLIRILDQSDPRDIFPRGLIVSFYDDNGQISSILTANRA
                     ERIVRKNMVVATDSVHLVSRNGEELLTNELIWDELKKEIYTNRFVRLTRPGEIIYSFG
                     FRSNQDFTKYELNALSGRLKKDNLDKDF"
     gene            complement(6523..7758)
                     /gene="porV"
                     /locus_tag="PKV31_03510"
     CDS             complement(6523..7758)
                     /gene="porV"
                     /locus_tag="PKV31_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNB91953.1"
                     /translation="MKKFIQFLVFAITLFMTSGATAQSSGTCVESPIGSNHWIEPDGS
                     PCRNSISTVVSFLRIVPDARTSGMGSVGLATSPDANAMHFNAAKLASSTKDGGLSMSY
                     VPWLKGLGLNDIFMAYLTGYYKFSKKEAIAASIRYFSLGDFTFTDTQGNIIGYGKPNE
                     FEIAVAYSRALGDKLFGALTGKFISSNLATGQQLNGVDIQTARTGAADISIFYRTPLK
                     TNTKSNLALGVAITNLGGRISYTKASPVRDLLPANLGIGMAWDVDLDQYNRLTLGVDF
                     NKLLVPSPDPALTDDNNNGVPDYREKALIPAIFGSFSDAAGGFKEELRECMFGIGAEY
                     WYNDQFSIRAGYQYESPLKGNQKYFTLGLGLKYTTFGLNLSYLVPSGNTRTALDNTLR
                     FTLMFDFGTFEVDDDPNIK"
     gene            complement(7852..>11450)
                     /gene="porU"
                     /locus_tag="PKV31_03515"
     CDS             complement(7852..>11450)
                     /gene="porU"
                     /locus_tag="PKV31_03515"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNB91954.1"
                     /translation="PYFNTQLPLDLPYREGLRNVEPILETQKGRVENRIEITFLPGRW
                     TSLTQFQLLDSIQFVLEVEDWPETRSNPPKFAEHSILQTIPTYKFRSADAGIYRIDNQ
                     FMADLGINTSGIDPSKIKIFTTNPGPLPEKNNLPRTDDLAEVAIQVIGGDDGKWDASD
                     YILFYSPGPHRWNKESDSTFTRVTNIYEDYKYFFIQVEGAPGKRIVLKDGGQAFDVES
                     DIYDHIEHYEVDKVNLLREYAKIGGGQLWLDELITGSNRTKDLSGLLKVDNLVAGETG
                     YVKMAFAVRSKSRSVISMIADNQAAEIQFETTRIEDSESRVASYNSKKSAFTQAGGPL
                     KISFDFPQVATESLGWMDYVEVNIKRYLSKPQSAMAFRLSNPGITGNIRYNLTGLSST
                     DAVWDISDLFNVTQCKISPTGGSGSIVAPYPQQREFICFGTNDIKTPEKIGLVTPQDI
                     HGMATPDMVIIYHAKFAEEARNYANHRSSISGIDVAIVDVDHIRNEFSGGGQDPTGIR
                     DMARMFYYRNPEKFRYILLLGDGSYDYRGLTFNATSKKENSNYVPVYEFPRAILEPIR
                     SIPSDDYFGMLDEEDGEIEKGTIDVYVGRFPVYDKSQAQSIINKIISYEKNKDSFGDW
                     RNYLMLISDDWDNVNSDDFIVQSEKIFNKIQSSNPGINFSKIYLDIFKQESNLGGQSY
                     PTAVSQMNDQYNKGVLIANYVGHGGVDGLAQERIIDRLTLNGMKNSNRLPLLITGTCS
                     FATYDDPDITSVGKLCITKPDAGMIGLMTTTRSVYITPNEVFINKLFQVIYQKENGRY
                     LTNGEILAKAKNLSSGADRLAFVLLGDPAMTLAFPRENILLETVNGAPIDTAKPDTIK
                     AMELVSLAGSVRAEDGRVIEDFNGTLTLTVFDKAYTLYSRGNDGNGANYPASFQNNIL
                     FKGKATVTAGKWNISFYLPKDINYAIDYGKLSFYADNGIYDAGGYSHQVKIGGESSNP
                     ITDNQPPVIDLYMNTLNFKSGEIVNTTPLFIARLSDDYGINVSSSSIGHEIVSTLNND
                     ATKEKIMNDFYVADKDEYNKGEVRYQFDQLDPGNYTLRLRAWDISNNKAEAEIEFIVT
                     NSEQNSISHLLNYPNPFTTSTAFMFEFDSQNNTIEAEIAIYSVSGKLVKTIREPLSPS
                     GKRFRSDLWDGKDQYGDQLAKGVYLYKVKIYNSFDTAKKVISSDFQKLVILK"
BASE COUNT         3063 a         2405 c         2595 g         3387 t
ORIGIN      
        1 aactgaacat aatcactgac agaaaggttt tctgctctct gattgaaaaa cgaatctgcc
       61 aggttggatt cgtctgcgaa aagtgttttc aggttgttgc gcaacatttt cctacgcatg
      121 gcaaagcttt gtttcaccac agttgtaaat gccttgatgt ccttaattcc tatatcttca
      181 ttcctccgta acaactctat gacgagcgaa tcgaccttgg gaggcggatt gaaggcggat
      241 ggcggcacgt ggattatctt ccgtccatgg taccaggcct gtgtaaggac acttatcaca
      301 ccataatcct tgcctcccgg tggagcaatg atccgctctg ccatttcctt ctggaacata
      361 cccaccatct gagggatcaa ctccttgtaa tgaatgattt gaaaaacaat ctgtgaagag
      421 atattgtagg gaaaattgcc tataatatta aagctttttc catcaaaaac gctattaaag
      481 tctagccgca atacatcgtt ttgaatgata tggtccgata atgccggaaa ttttttattc
      541 aacaagataa ccatatcgcg gtccagttcg acgcacttta agtctaggtt ccggcccatc
      601 agtatttcgg tcaacacgcc cggccccggt ccgatctcca ggactctccc ttgggtcgag
      661 gtcatgtcac atgcatggac aatggcttcc gcaatgtcct gtctgtgcag aaaatgttgt
      721 cccatggatt ttttgggaga tggaaattta ctgaaactca tgttttatgg aataaagagc
      781 gaagatacac aggaattgag agttattagg attgattttg cgagtcttct tcataagact
      841 gataaagagc cgaaacatta atgagcgtaa aatgttttac atcttcagaa acattttaga
      901 aataagtaaa atgcatatat ccggtcgatt tatcattcca ttcctcctgt ttgtcctgat
      961 agatatatac gcattccagg catttcgggg tagcttgcgg agtttgaatc ccgatttccg
     1021 aaggtttctt tattatggat actggatctt atctgctatt gcctatctgg cagccacagc
     1081 catgattacc ggtatactga cagaaagaca acattttctg atggtatatg gaaggacgct
     1141 tttgatgatt ctttttaccg ttaaagcatt atcgattccc tttctactca tcgatgacat
     1201 acgtcggggg acattggcag tgattcaata cttttctcct gctaattcat acgatccaag
     1261 ccgatcccga tttctttcta ccttaagtct ggcgatgggg gccattcctt ttgccctgct
     1321 atcttatggg gcagtgcgca atccctatcg atacaaggtt cgaagacata aggtttttat
     1381 cgaagatctg catccggacc tggagggttt caagattgtc cagttttccg atgtccattc
     1441 cggaagcttt gtttttaagg aaccgatagc ccatggaatt gatctgataa ataaggaaaa
     1501 cggtgacctg attgtgttta caggggatat cgtcaatacc ctggcagaag agatggacgg
     1561 aatgtacgaa atattcagtc gggttggtgg accgggtaat acagtctatt ctattcttgg
     1621 caatcacgat tatggtaact atcatcaatg gaacagcaag gctgaggagc aaaagaattt
     1681 tgaagaggta aagaatatac accataaatt gggctgggat ttgttgctca atgaacatcg
     1741 tgttttgaca cacaaagcag cacgaatagg acttgtagga gttgaaaact tttccgcact
     1801 acctcaattc cctaaacatg gggatttgaa aagggctgtt gatggaatgc ccggcgttga
     1861 tgtaaggatt ctactgtcac atgatccaag tcattgggac tttgaagtga tcaaggaaca
     1921 taaggacatc gatctgacac tgtcaggaca tacacatggt ttccaattcg gtgtggagat
     1981 tcccggctgg ttccgatgga gtccaagcag gtttgtatat aaacaatggg ccggattgta
     2041 tcaaaaaggc aggcaatatc tctatgtcaa ccgtggcttt ggttttcttg gttatgcagg
     2101 aagagttggc atcctccccg aaataaccac tttggtcctc accggaaaga aggagtaggc
     2161 gaaagcgtta aagtttaccc aaaatcctgc aatctcaccc tggatttctt ttaaatctca
     2221 ttggattgtg tatctttagc ttggttttat ccttgccgtc cacacatctt aaacctatgc
     2281 cctatgaaag catggattgc atttttgctg tatttccttc ttttgggaaa ttgccatata
     2341 ctcaaagcgc aagccatcaa cgatgactgc tttagtgcca ctgagctgac caatgtacgt
     2401 agctggtgtt catccaatgg ctttttcaac aacttttctg ccacaccatc aggatatggc
     2461 cctgcgactt gttttagcag ctccggacat gatgtatggt tttcatttac ggctatagca
     2521 acagatatta ccattctggt caagccgggg agtcttcaga agccggaaat cgccctgtat
     2581 gccgggattt gtcagggcac tataggcgaa ttgaattgta agtccaatct tgtgactgga
     2641 ggggcacttg aattgtataa gggaggtttg tttccgggaa cagtctattt gattcgggtc
     2701 cagggggagg atgcctcaga aggtacattc gacctgtgtc tggacaatta ttttccgcct
     2761 gtcaagcccg gaagtgatat tataactgca tcagtacttt gtgataaaag ttctttcatt
     2821 gtgcagaacg ttacaggatc aggtagtgtg cccaatgagt ttaaggacgc tccatgcctg
     2881 gcagatggat ttaccaattc cgaaaccaat tctacctggt ttaaatggac ttgtaaggat
     2941 gccggtagtt taacatttgt tctcactcct ctgaatgcgc cggatgatct ggatttcgct
     3001 ttgtatgagt taaatgggga tctgaacaca accaacagaa cactcctcag atgtgaggct
     3061 gcttcctgca agggtcagac aggtcttaag gaaggagcga cagatatcag tgagccacct
     3121 aactgtgata aaggtcagga caactggttg gcacccgttc aacttgaagc cggaaagaat
     3181 tatttgctcg gtatcaataa cttcacgatt ggaggcaatg gtttcaggat tgaatttgga
     3241 ggaacatcca ccttcgaagg tccggttgcg gactttgaaa tcctcagtga tacagtattt
     3301 tgccgggaag aacaaattgg cataaaggac ctctcgcata acagcactgg aacaattgaa
     3361 aagattcagt ggatcttcgg gcaggatgct acaccttcca ttgattccat tcagggagac
     3421 aaaacaattt cttacctgaa gcgcggtcaa aagttcatcg ttcagactgt taccagcgac
     3481 aagggctgcg tggtttctac catcaagcct gtcgaaatcc agtgctgtgg ccctcttcac
     3541 gagatgaatc tatacagcga tacaaccata acattgggag attcggttga tttgcacgct
     3601 gaagcctttt tggaagggaa tcagatcttt tatttctggg aaccgcctgg cctgatacaa
     3661 tgtcagggct gtcctgctaa taatatcaag actttccacg atcaggtgat tgtcgtcaag
     3721 gcaacagacc agtataactg tgaggctaaa gattctatcc agattagggt ggatgtcaaa
     3781 agaccgattt ttttccctaa tgtattctca ccaaactatg atggaatcaa cgaccgcttt
     3841 accggatatg gtaactttgt agtcaggacc attgactatc tgcggatatt caatcggtgg
     3901 ggtgctttgg tttgggaagg aaggaatatc ccggcaaatg atgaaaagta tggatgggac
     3961 ggtactttca aggggcgtga agtcaatccg gatgtgtatg tttatattgc caaagtgact
     4021 tttatcgatg atcaatcatt tatttacgaa ggttctatta ccgtggtcag gtaggttgct
     4081 ccgcattgta agtgttctgg ctttattcct ttggccggca aaccttgtat tctgccaaac
     4141 catcaatgat gaatgcaaga aagcaatatt tattcccaat gttaaggaat attgttccgg
     4201 ggacacaacc ttcagttttt tgggttccac cccttccaat aatggatttt ccttatgtca
     4261 gaccgataat ggtaatgatg tttggttttc cttccatgtt acagcaactg acgttgtcat
     4321 caaaatttta tgtaaggagg caaatgactt gcatgcttcc ttatattccg gagattgcgg
     4381 cagcctgaat gaagaaacat gtggaaggag agaggccgga caaaactttc ttcagttgta
     4441 caaaggaggc ctagtgcccg gcaccagact atatcttagg gttgattcgg aaaatggcac
     4501 gaatttgaaa tttggattgt gcatcaataa ctttttctca ccagctttgg atggtagtga
     4561 ctgcaaaact gccaatctga tttgctcaaa ggaatccttt acagtaagga atgtacaggg
     4621 cttcggagag gtcaatgaac tcgggaatgc accatgtttt agccacgcca atgagtccaa
     4681 cagcatttgg tttagatgga tatgcaggga tccgggcaca tttacattta aacttattcc
     4741 cttgaaagag gcggacgata ttgactttgt cgtgtataaa gtccgggggg atgcattcgg
     4801 gtgtgatttg gaattggtgc gttgtatggc taccgggata tcaccggtca attgcaccaa
     4861 ctaccctaat tgttgtggct ctacgggcct tcgtgacgga gaaaccgatg agtctgaggg
     4921 cgcaggttgt ggtccggacc gtaataattt cctgaaaccg ctggacatgg aaagcggaga
     4981 ggtatatgcc atattaatta ataattacac atcacaaaat gaaggctttg acttcaatat
     5041 gggcggaact gcaaccctga agtcattaca agccgatatc gacatcataa agcctgatgc
     5101 actctgccgg gaagagagtc ttgaagtgaa ggataaaatc atcaatgacc ttggtaccat
     5161 caaatcactt ctttggacat ttaccccgga tgcatccatc agtacttcac agggagccgg
     5221 acctgtatcc gtccactttg acagatcagg ttataagaca atctcgctta ttgtggaaaa
     5281 cgatgccgga tgtaaggcat acgcgcaaga taccgttttt gtattttgct gtggaggtac
     5341 acttcgggct gatattctgg gtaatacctt tgttgattat ggtgaaatgg cagaatttgg
     5401 tgttcaattt gttttggaag gcgaagatgt gaagtaccaa tggacacctg ccggtcaggt
     5461 caattgtgat acctgctccc tgataaccct tgatccggtc tatgaggatt tccctttatg
     5521 ggtaaccgta agtgatgaaa atggctgtaa ggcggaaaac cgggtgttca ttaagacagg
     5581 taagccggaa gtttttgctc caaatatttt ttcgcccaat catgacggta tcaatgatcg
     5641 gtttatgctg agcttcgctg aaggtatcca cagaatccgc tcattgagga tttacaaccg
     5701 atggggtgga ttggtgtacg aaggtagaaa tatggataag agtgatgccg atggaggatg
     5761 ggacggcagg tttaaggatc gagacgctgc acccggcacc tatgtttatt atgcagagct
     5821 ggaaacgcga ggcggtaaaa caatggtaat aaaaggaggc ttgacattga ttagataaac
     5881 atcagtctat gtcaatgaaa atttacaact ttgcagcatg aatatttcct ttcgtacttt
     5941 tttgggcctt tgtctgcttg ccttgtcttt tcaggcatgt gaaaatgacc tggcagaggt
     6001 gcgcagagtg acaggggtat ctgtatccgg aagttatgaa gaaggagtcg atggagaaat
     6061 tctttacagt gattctgcca gactcaaggt ggtcatcaaa tttgatacgc tgattcgtat
     6121 tcttgaccag agcgatccgc gtgatatctt ccctcgtggc cttattgttt cattttatga
     6181 tgacaatggt caaatatcaa gcatattgac tgctaacagg gctgagcgga ttgtaagaaa
     6241 aaatatggtg gtggcaacgg attccgtgca tcttgtcagt agaaatggag aagaactact
     6301 gaccaatgaa ttgatttggg atgaattgaa aaaggaaatt tacaccaacc ggttcgttcg
     6361 tcttaccagg ccgggagaaa ttatttacag cttcgggttc agatctaatc aggattttac
     6421 gaaatacgaa ctaaatgccc tttccggacg cttgaaaaaa gacaatctgg ataaggactt
     6481 ttaatttgga ttattcccga aagacttgcc tttgtccgat gcctacttta tattaggatc
     6541 atcatcgacc tcaaatgtgc caaaatcaaa catcagtgtg aagcgcaagg tattgtccaa
     6601 tgcagtcctc gtattgccgg agggcacaag ataggacaga ttcagcccaa aagtggtgta
     6661 tttcaatccc agcccaagtg tgaagtattt ttgattacct ttcaatgggc tttcatattg
     6721 ataaccagct ctgatagaaa actgatcgtt gtaccaatat tcggcaccaa taccaaacat
     6781 acactctctc aattcttcct taaatcctcc tgctgcatcg gaaaaagatc caaatatagc
     6841 cggaatcagg gctttttccc tgtagtccgg tactccattg ttattatcat cggtaagggc
     6901 aggatccgga cttggcacta agagtttatt aaaatcaaca cccaatgtaa gtctgttgta
     6961 ttgatccaga tcgacatccc atgccattcc gataccgagg ttagccggga gtaagtctct
     7021 taccggtgaa gctttggtat aggatatcct tccgcccagg ttggtgattg ctacgcctaa
     7081 ggccaggttg ctcttggtat tcgttttaag gggagtccgg taaaatatcg atatgtcggc
     7141 cgctcccgtg cgtgctgtct gaatatccac accattgagt tgctgaccgg tagcgaggtt
     7201 ggatgagatg aattttccgg tcaaagctcc aaataatttg tcgcccaatg ccctgctata
     7261 ggctacagct atctcaaatt cattcggttt gccataacca atgatatttc cttgtgtatc
     7321 cgtgaacgta aagtctccta aagaaaaata tctgatggaa gcagctattg cttctttttt
     7381 gctgaatttg taataaccgg ttaggtacgc catgaaaata tcattcaatc caagtccttt
     7441 tagccatggc acatagctca tggacaagcc gccatccttg gttgatgaag caagttttgc
     7501 agcattgaag tgcatcgcat tggcatccgg tgatgtggcc agccctaccg aacccatacc
     7561 tgaagttctt gcgtccggaa cgattctgag aaaggatact acagttgata ttgagttgcg
     7621 acaaggtgat ccatcaggtt caatccagtg gttcgaacct atgggactct ctacacaagt
     7681 acccgaggac tgcgcagtcg ccccgcttgt catgaagagt gtaatagcaa atactaaaaa
     7741 ttggataaat tttttcatat tgaaaaatgt aaatatgtat tttatacaaa atggccgcaa
     7801 atataaactt tatattcgtc attttattgt gtcgtaaggc ccttttctgg tttatttaag
     7861 aattacgagc ttctgaaaat cagatgaaat gactttttta gcggtgtcaa agctgttata
     7921 aatttttact ttatataaat acacaccctt tgccaactga tccccgtatt gatctttgcc
     7981 atcccaaagg tcggaccgga atcgttttcc actcggactc aggggttcac gtatggtctt
     8041 gaccagtttg cctgaaacgg aatatatggc aatctcagct tcaatagtat tgttctgaga
     8101 atcaaattca aacataaaag cagtggatgt tgtaaaagga ttgggataat tcagcagatg
     8161 agaaatcgag ttttgctcac tgttcgtgac gataaattcg atttcagcct ctgccttgtt
     8221 atttgaaata tcccatgctc tcaatcgaag cgtataattt ccggggtcaa gctgatcaaa
     8281 ctgatatcta acttctcctt tgttatattc atccttatct gccacataga agtcgttcat
     8341 aattttttct tttgtcgcat cattgttaag cgtggatacg atttcatgcc ctatactgct
     8401 gctggaaaca ttgatgccat aatcgtcact cagccgtgcg atgaacaaag gggttgtatt
     8461 gacaatttct cctgatttga aatttaaagt attcatataa aggtcgataa ccggtggctg
     8521 attgtcggtg attggattgc tgctttcacc accgattttg acttgatggc tgtagcctcc
     8581 cgcatcatag attccattat cggcataaaa tgataattta ccataatcaa tggcgtagtt
     8641 gatatcctta ggcaggtaga agctgatgtt ccacttacct gcagtgactg tggccttccc
     8701 tttgaaaagg atattgttct ggaaggaagc cggataatta gctccgttgc cgtcattgcc
     8761 acgagagtat aaggtatatg ccttatcgaa tacggtgagt gtgagagtcc cgttgaaatc
     8821 ttctattacc ctgccatctt cagccctgac actccctgca agagagacaa gctccatcgc
     8881 ctttattgta tccggtttag ctgtatctat cggcgcacca ttgacggttt ccaataagat
     8941 gttttccctt ggaaatgcca gtgtcatcgc cggatctcct aaaagtacga aggcgagcct
     9001 gtcagcccct gaagaaagat tcttcgcttt ggctaatatt tccccattgg ttaagtaacg
     9061 tccattttcc ttttggtaga ttacctgaaa taatttattg ataaacactt cattgggcgt
     9121 gatatatacc gagcgggtcg tagtcatcag gcctatcatt cccgcatccg gtttggtgat
     9181 gcacaacttg cccacagagg tgatgtcggg atcgtcgtaa gtggcaaatg agcatgtacc
     9241 ggttatcaac agaggcaacc tgttgctgtt tttcatccca ttgagagtaa gcctgtcgat
     9301 gattctttcc tgagcaagac catcaacacc accatgaccg acatagttgg ctatcaggac
     9361 gcctttgttg tattggtcat tcatctggct gacggctgtt ggatagctct gtcctcccag
     9421 attagattcc tgcttgaaaa tatccagata aatcttcgaa aaattgatac cgggattgct
     9481 gctttggatc ttattgaata ttttttcgga ttgtactatg aaatcatcac tgtttacatt
     9541 atcccagtcg tctgatatca acatcagata attgcgccaa tctccgaagg aatccttgtt
     9601 cttctcataa ctaatgattt tgttgatgat ggactgagcc tgacttttat catagacagg
     9661 aaaccggccg acataaacgt caatcgttcc tttttcaatt tcaccgtctt cttcatccag
     9721 catgccgaaa taatcatcgg atggaataga tcggatgggt tcgaggattg ccctggggaa
     9781 ttcatataca ggtacataat tggaattctc cttcttggag gtggcattga aggtcagccc
     9841 ccggtaatca tagctcccat cacccaacaa aagaatatat ctgaattttt ccggattgcg
     9901 gtagtaaaac atacgtgcca tatcccggat accggttggg tcctggccac cacctgaaaa
     9961 ctcattcctg atatggtcca catcgacaat agcaacatca ataccggata ttgagcttct
    10021 gtgattggcg taatttcttg cttcttccgc aaatttggca tgatatatga tgaccatgtc
    10081 cggggtggcc attccatgaa tatcttgcgg agttacaagg cctatcttct cgggtgtctt
    10141 gatgtcgttt gttccaaaac agataaactc ccgttgttgg gggtagggtg ccactatgct
    10201 gccacttccc cctgtggggc tgatcttaca ctgtgtaaca ttaaacaagt ctgagatgtc
    10261 ccaaactgca tcggtagaac ttaagcctgt cagattgtat ctgatgttgc cggtgatccc
    10321 cgggtttgaa agcctgaaag ccattgctga ttggggcttt gaaagatatc ttttgatgtt
    10381 gacctcaaca tagtccatcc atccaaggct ttctgtagcc acttgaggga agtcgaagga
    10441 gatcttcagt ggaccgcctg cttgagtaaa tgcgcttttt ttcgaattgt atgaagctac
    10501 ccggctttct gaatcctcaa tcctcgtggt ctcgaattga atttctgcag cctgattatc
    10561 ggcaatcatg ctgatgactg accgcgactt gcttctgacg gcaaaagcca tcttgacata
    10621 gcctgtttct cccgcaacca gattatccac cttcagcaag cctgacaggt ctttggttcg
    10681 attactcccg gttatcaatt cgtcaagcca tagctgccct ccaccaattt tagcatattc
    10741 ccgaagtaaa ttgaccttat caacctcgta atgttcaata tggtcataga tatctgattc
    10801 cacatcaaaa gcctgccccc catccttcag gacaattcgc ttacccggag caccttctac
    10861 ctggatgaaa aaatatttgt aatcttcata aatattggtc acccgcgtga aagtactgtc
    10921 actttcctta ttccacctgt gaggtccggg agaataaaaa agaatgtagt ctgatgcatc
    10981 ccacttgcca tcatctccgc ctatgacctg aatagccact tctgccagat cgtctgtcct
    11041 tgggaggttg tttttttcag gtaaaggacc tgggttggta gtaaatatct ttattttgga
    11101 tggatcgatt cccgatgtat tgatacccag atctgccata aattggttgt caatccgata
    11161 aattccggca tcggctgatc tgaacttgta ggtgggtata gtttgaagga tggaatgttc
    11221 tgcgaatttt ggaggattac ttcgggtttc cggccagtcc tcaacttcta agacgaattg
    11281 gattgaatcg agtaattgga attgggtcaa ggaggtccat cttcccggta aaaaagtaat
    11341 ttcaatacga ttttcaaccc tgcctttttg tgtttcaagg ataggttcaa cgttgcggag
    11401 gccttcccgg tagggcaagt caagaggcaa ttgggtgttg aaatatggct
//
LOCUS       DAOQMC010000053        11372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2209_length_11372_cov_4.704250, whole genome shotgun sequence.
ACCESSION   DAOQMC010000053 DAOQMC010000000
VERSION     DAOQMC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11372
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2209_length_11372_cov_4.704250"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKV31_03520"
     CDS             complement(<1..267)
                     /locus_tag="PKV31_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNB91955.1"
                     /translation="MLNNIVYSLRDKDMVTMIMYGIPNCNSVKKARVWLEHHNVEYTF
                     HDYKKLGISSEKIEAWLGQIPLERLLNKAGMTYRNLNSIEKESAS"
     gene            350..1114
                     /gene="map"
                     /locus_tag="PKV31_03525"
     CDS             350..1114
                     /gene="map"
                     /locus_tag="PKV31_03525"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNB91956.1"
                     /translation="MLIKTKEELDGIRKAGQTAGEILKKMRQYCKPGLSTKEIDLYGG
                     RLMKEYGVRSAPILAYNFPGFTCISVNDVVAHGVPSTQTILREDDLINIDVSIEQDGF
                     WADNGGSFTLGQQNSPYDHLIDASRDILKQAIFRIKHGMRVSELGGYIHDSAKKVGYT
                     VIKNLTGHGVGRSLHEEPRSIANYREAFNFRRFRENEVVAIETFISTKSEFTKTDNDG
                     WTLRGDKGGFVAQHEHTILVTRQEPEILTLSNEIWS"
     gene            1221..2147
                     /locus_tag="PKV31_03530"
     CDS             1221..2147
                     /locus_tag="PKV31_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNB91957.1"
                     /translation="MEYIDYYKILGLDKTATSQEIKSAYRKLARKYHPDLNAGDKEAE
                     KKFKEINEANEVLSNEENRRKYDKYGKDWKHAEEIERMRRQQGSRASSGNQGYGKGYE
                     GFSGFSDSGFEDDDFSDFFHSMFGRTSGGRSRTSTRFKGQDYNAVLHLNLTDVYHSKQ
                     QVIEVNGKKLRMTIPAGVEDGQTIKIAGKGGPGANGGPDGDLYIRFEIHNDTDFRREG
                     ANLYNQVEIDLYTALLGGEVTVKTFNGQVKLKIAAEMQSGTKVRLKGKGFPVYKEKEK
                     FGDLIITYLIKNPTNLSEQEKDLFRQLKKLRS"
     gene            2147..2446
                     /locus_tag="PKV31_03535"
     CDS             2147..2446
                     /locus_tag="PKV31_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HNB91958.1"
                     /translation="MADKYILIRTICTHYGLEQDFLDRLNETSLISYHIKDQDIYINK
                     DQIGNLEKIIRLHYDLGINFEGIDVIFNLMERITEMEAELKLLKSHFSSFNDNLL"
     gene            2762..3304
                     /locus_tag="PKV31_03540"
     CDS             2762..3304
                     /locus_tag="PKV31_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNB91959.1"
                     /translation="MEKDSEQLLSKEEIHLDKLRRIVQKSIEEENLIMENLLHPPSEI
                     LTIGQKISDKVARFGGSWAFIITFFIILTFWIILNYTLPKSDQFDPYPFILMNLLLSC
                     IAAFQAPIIMMSQNRQEEKDRKQNINDYMVNMKAEMEIKALHQKIDLLLEEQIKVLFE
                     SHAKQLAILESLQEKIEKQS"
     gene            3307..4506
                     /locus_tag="PKV31_03545"
     CDS             3307..4506
                     /locus_tag="PKV31_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB91960.1"
                     /translation="MPKLSEKATLMPPSPIRKLVPYAETAKRQGKHVIHLNIGQPDIE
                     TPEIAREAVKHADIKVLEYSHSAGIESYRKGLAAYYSANGISVTSDEIIVTTGGSEAL
                     LFAFMTCLNPGDEIIIPEPFYANYNGFAVSAGIHVVPVTASIDNGFALPPISEFEKKI
                     TPKTKAILICNPSNPTGYLYSREELEHLREIIQSHDLFLFADEVYREFCYDGATPFSV
                     MNLKGIENNVVLIDSVSKRYSMCGARIGALVSKNKEIMQAALKFGQARLSPPTYEQIA
                     AEAALDTPKSYFDDVIKEYVGRRDLLVDGLNKIKGVRCPKPNGAFYCIAEFPVEDTEH
                     FCQWLLESFEFEGNTVMMAPLSGFYATKGLGKKEARLAYVLKKEEIEAAVRCLEKALE
                     VYPYTSY"
     gene            complement(4742..5638)
                     /locus_tag="PKV31_03550"
     CDS             complement(4742..5638)
                     /locus_tag="PKV31_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNB91961.1"
                     /translation="MKKNIHGRREVLKKLSLGGLALTGVGKLSAFNNDSEESLAWKGN
                     IRHSVCQWCVSFLTIEEMALWIKSIGVGAIDLISPKDWNILKKYNLECSMCYIDGKVS
                     LTEGWAQRENHARLVEDYIKSIPMVKDAGYTNLICFSGNRKGMDDETGLKNCEEGLKK
                     IMSLAEKNNVIIQMELLNSKVDHHDYMADKTAWGVELCKRIGSPNFKLLYDIYHMQVD
                     EGDVIHTIRDNHQYIGHYHTAGVPGRNEIDDTQELYYPAIMKAIVETGFKGYVAQEFI
                     SKGKEKDEKLQAFKRGIEICDV"
     gene            complement(5635..6864)
                     /locus_tag="PKV31_03555"
     CDS             complement(5635..6864)
                     /locus_tag="PKV31_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HNB91962.1"
                     /translation="MKPTVRIQLNSMMFLNFFVWGIWFVTLGTYLLKGDIKASDAQVG
                     LAFGTQCLGATIAPFIIGLIADKYFAAQKILGILHLAGAVLMYLISGSKEFTGFYTLL
                     LIYMVIYMPTLALCNSIALNQVSDAEKEFGGIRLWGTIGWIVAGQVVGFLALEKNPGD
                     LVMTFKIAGAISLVFGIYSFFLPDTPPPKAGKDVSFREIVGLDALKVLKIKNYAVFFI
                     ASILICIPLAFYYGQANIFLNEIGMQGAAQKMSLGQMSEAVFLFLMPWFFRRLGVKYM
                     LLVGMLAWVVRYLCFAYGDLDSGLWMLYLGIILHGICYDFFFVTGNIYTDQVAGPQVR
                     SSAQGLITLATYGLGMYIGFWAAGAIAGANVILDAGGEVTNHHWDKVWLFPAMFAAGV
                     TVLFLLFFKKSEKQDIA"
     gene            7027..8172
                     /locus_tag="PKV31_03560"
     CDS             7027..8172
                     /locus_tag="PKV31_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB91963.1"
                     /translation="MFRKMRVAMIGGGRNAFIGSIHRIAIQMDGLAEIVAGALSANPE
                     NALASGKDLFLPEDRIYTDFRTMIKKESASPSEIRPDIICIVTPNHMHFEPAMLALQN
                     GFHVAIDKPITFSTEEAIRLKEVLESSGKQLLLTHVYTGYPMIKQAREMIQSGVLGRI
                     RKVFVEYPQGWLSAPLEQENNKQASWRTDPTKSGKAGAMGDIGTHAFNLAEYVSGLSV
                     YKICADVNTIVPGRLLDDDGAVLLRFEGGASGVLMATQVATGEENNLRIRVYGEKGGL
                     EWQQADCNSLRVMWPDKPAEIFRTGAGYSGAMAVHNSRTPAGHPEGYLEAFANLYRNF
                     FHTIAASDKQESEKYLDFPGIEEGIRGMKFVDKVIESGVSEQKWLTF"
     gene            complement(8332..10854)
                     /locus_tag="PKV31_03565"
     CDS             complement(8332..10854)
                     /locus_tag="PKV31_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB91964.1"
                     /translation="MLSQQVAIDNVKPVARAVQHAKSIKAFPKVDLFVEDLSLDVRDK
                     YPDMLSQFSYLKLRKEALNSFLNTRPDAINIQVPYLSQNIQLELVRVDVLSDDFKTYT
                     SDSPEKAVNYSPGLYYRGIVEGDVSSTVAISFFEDDVIGMISTEELGNITFNKMEDAD
                     RYLIYSDRDLLVKNPNFCNTEEPENYAEQVRQYMNNSIEVRDGKCVKVYIECDYALYQ
                     NKGSVTNVNNWISAVYNNVATLYANEQISTQISSTFVWTSSDPYSKTNSYTALTQFRN
                     NRPSFNGDLAHLAALGGQNIGGVAWVDALCTSYKYAYSNIQATYQTVPTYSWTVEVMT
                     HEMGHNLGSPHTQSCSWPGGALDNCYTTEGGCPPGPPPTNGGTIMSYCHLTSYGINFN
                     NGFGTLPGNLVRSRVNAASCLGTCSGGGTCNAPSGLVVTNITNTSAVVSWNTVSGATA
                     YQLQYRATGGSWTVLSQQTTTSVTLTGLAGSTTYETQVRSICSGGNSAYSAIVTFTTS
                     GGSGCSVPTGLAATNITNTSATISWNSVSGAVNYTLRYKLTSSGTWNTYTTSSTIVNM
                     TNMTPGTSYDVGVRTNCSNGSSNYSATVTFTTTGGGASYCTSKGNTSSYEWIDLVKLG
                     SINNVTGSNGGYGDFTALNTTLSAGSSYTMTLSAGMNGSYTEYWTVWIDYNRDGDFLD
                     PGEKVTSFSSSSKGNINRSFSVPTTASNGPTRMRVQMKYGSYGTSCETFSYGEVEDYT
                     VTISGGSNLPSAITNTITDYMVYPNPSSDVFTAQFEAGSSMEATISIMDANGRVVSDQ
                     LVNTIAGKNIVPLETSGYLVPGMYLIRIVTKFGHQAIRVVKM"
BASE COUNT         3596 a         2434 c         2230 g         3112 t
ORIGIN      
        1 cgatgctgat tccttctcaa tggaattaag gttccgatat gtcataccgg ccttattcag
       61 caatctttcc aaaggtattt gaccgagcca cgcctctatt ttttcgcttg aaatgccaag
      121 cttcttgtaa tcatggaaag tatattcaac attgtgatgc tcaagccaga ctctggcttt
      181 tttgactgaa ttgcagttgg gaattccgta cattatcata gtaaccatat ctttgtctcg
      241 aagtgaatat acaatattat ttaacattcc accatacaat cttactaacc caataacttt
      301 aaccggggat attttgttaa accatctccc aaaacatgta acctaaaaaa tgcttattaa
      361 aaccaaagag gaacttgatg gaatcaggaa ggctggccaa acagccggag aaatcctgaa
      421 gaaaatgcgc caatattgta agccgggact atcgacaaaa gagattgact tgtatggtgg
      481 caggttgatg aaagaatatg gcgtacgatc agctcctatc ttagcataca actttccggg
      541 ttttacctgt atcagcgtca acgatgttgt agcccacggc gtcccttcaa cccaaacaat
      601 cctaagagaa gacgatctca tcaacatcga tgtatccatc gagcaagatg gattctgggc
      661 agacaatggc ggctctttta cccttggaca acagaactcc ccgtatgatc atctgattga
      721 cgcatccaga gacatactca agcaggctat tttccgtatt aaacacggta tgagggtgtc
      781 agaactcggt ggttatatcc acgattcagc taagaaggtc ggatacaccg tcattaagaa
      841 tcttacaggc catggtgtcg gacgcagtct tcacgaagaa ccacgctcta tcgccaatta
      901 cagggaagca tttaatttca ggcgattcag ggaaaatgaa gtagtcgcga tcgaaacatt
      961 tatttccaca aaaagtgaat ttacaaaaac ggataacgac ggatggacac tcagaggcga
     1021 caagggtgga tttgttgccc aacatgaaca cacgattttg gtcacacgac aggagccaga
     1081 aattctcacc ctaagcaacg aaatatggag ctgaatgtgc aacaaaagtg taaattttgt
     1141 ggtttatatc actgatataa gtcataagac agatattatt ttcaacaatt tcatcattta
     1201 acccaaaatc actttacttc atggagtata tcgattatta caaaatactc ggattagata
     1261 aaaccgcgac atctcaagaa ataaaatcgg cttatcgcaa actggcaaga aaatatcatc
     1321 ccgacttgaa tgctggtgat aaagaagctg aaaaaaaatt taaagaaatc aacgaagcca
     1381 atgaggtcct gagcaatgaa gaaaacagaa ggaaatacga taaatatggt aaggactgga
     1441 agcatgcgga agaaatagaa cgtatgcgca gacaacaagg cagtagagca tcctccggaa
     1501 atcagggcta tggtaaggga tatgaagggt tcagtggatt cagtgactca ggctttgagg
     1561 atgatgactt ttctgatttt ttccattcta tgtttggcag gacttcaggt ggaaggtcaa
     1621 ggacttcgac acgatttaaa gggcaggatt ataatgccgt attacatctc aaccttactg
     1681 acgtgtatca ttcaaagcaa caagtaatag aagtcaatgg caagaagctc agaatgacca
     1741 tcccggcggg tgtggaagac ggacagacca taaagattgc tggcaaaggt gggccgggcg
     1801 ccaatggcgg accggatggt gacttgtata taagatttga aatccataac gatacagatt
     1861 tcagacgcga aggcgccaat ctctacaacc aggtcgaaat tgatctatat actgctttac
     1921 ttgggggtga ggtcaccgta aaaacattta atggccaggt aaaactgaag atagccgcag
     1981 aaatgcaatc cggaacaaag gtaagactca agggcaaagg atttcctgta tataaggaaa
     2041 aagaaaaatt cggcgacctt attataacct atctaatcaa gaatccgact aacctctctg
     2101 agcaggaaaa agatttattc cgccaactta aaaaacttag atcataatgg ctgacaagta
     2161 tattctaatt cgcaccatct gtacccatta cggtttggaa caagattttc tggatcgact
     2221 caatgaaacc agcctcatca gctatcacat caaggatcag gatatttata tcaacaaaga
     2281 ccaaatcggc aacctggaaa aaatcatacg gcttcactat gacctgggaa tcaatttcga
     2341 aggcatcgat gtcatcttca acctcatgga acgaattact gaaatggaag cagagttaaa
     2401 actcctcaag agtcattttt cttcctttaa tgacaatctt ctttgacctg cctgttactt
     2461 gaccaataag acctcccgac atatattggc gatagcagcc aatttaccgg gattctgcct
     2521 gtttatattt ccattgttgc acttttccaa tttcccggga aatagtcaaa tagatgagtt
     2581 cacctctgtt gtaggcttga ctttaaccat ttcgtccata ttttccttct tttcgatcaa
     2641 gagtgaatac gacaaaaaga acaaaaacca gaaaatattg ccgatatatt cttcataata
     2701 tccttaatag gcatattttt gattattgtt tttataaatt taatttctgg tttaaataaa
     2761 tatggaaaaa gattctgaac agttactttc caaagaggaa atacaccttg acaaactacg
     2821 caggatagtt cagaaatcca ttgaagaaga aaacctcatc atggaaaact tactccatcc
     2881 cccatctgag atactgacta tcggacaaaa aatatcagat aaggtagccc gatttggtgg
     2941 aagctgggcc ttcatcatca cttttttcat catattgact ttctggatta tactcaatta
     3001 tacactgccc aaatcagatc agtttgaccc ctaccctttt atcctgatga atttattatt
     3061 gtcttgtatt gctgctttcc aggctcccat catcatgatg agccaaaacc ggcaggaaga
     3121 aaaggacagg aaacaaaata tcaacgacta catggtcaac atgaaggctg aaatggaaat
     3181 caaggcatta catcagaaaa tcgatttatt gctcgaggag caaataaagg tattattcga
     3241 aagtcacgcc aaacaattag ccatcctcga atcactccag gaaaaaatag aaaaacaaag
     3301 ttaaatatgc ctaaattatc agaaaaagct accttaatgc caccatcacc catcaggaaa
     3361 cttgtgcctt atgccgaaac agccaaacgc caaggcaagc atgtaatcca cctgaatatc
     3421 gggcaacctg atatcgaaac tccagaaata gcgagggaag cggtcaaaca cgctgatatc
     3481 aaagtgttgg agtattcgca ttccgcaggt atcgagtctt acagaaaggg ccttgctgct
     3541 tattattcag ccaatggaat ctctgtcact tccgacgaaa tcattgtgac cacaggaggc
     3601 tcagaggcac tcttgtttgc cttcatgacc tgtcttaatc cgggcgacga aatcataata
     3661 ccggagccat tctatgccaa ttacaacgga tttgccgtgt cggccggtat tcatgtcgtt
     3721 ccggttactg caagtatcga taatggtttt gccttgccac caatatcaga atttgaaaag
     3781 aaaatcacac ctaagaccaa agcaattctt atttgcaatc caagcaatcc cacaggctac
     3841 ttatattccc gtgaagagct cgaacatctt cgtgaaatca ttcaatcaca tgacttattc
     3901 ctttttgctg atgaggtata tcgtgaattt tgttacgatg gggccacccc attttctgtg
     3961 atgaacctaa aggggattga aaataatgtt gtcctcatcg attcggtttc aaaaagatat
     4021 tccatgtgtg gtgccagaat tggtgccctg gtatcaaaga acaaggaaat tatgcaagcc
     4081 gctttgaaat tcgggcaggc tagactttca cctccaacct atgaacaaat cgcagccgaa
     4141 gctgctttgg atacacctaa aagttatttc gatgatgtaa tcaaggaata tgtaggccga
     4201 agagatcttt tggtggacgg ccttaataag atcaaaggag tgcgttgtcc gaagccgaat
     4261 ggtgctttct actgtattgc agaattcccg gttgaagata cggaacattt ctgtcaatgg
     4321 ttgttggaat cctttgaatt tgaaggaaat acagtaatga tggcaccttt gagcggattc
     4381 tatgccacaa aaggcctggg aaaaaaagaa gccaggttag cctatgtact aaagaaagag
     4441 gaaatagaag ctgcggtaag atgtctggaa aaagctctgg aagtatatcc ttacacgagt
     4501 tattaaacaa atcagttttg gaacaagctt tcttaaacag aaggcttgtt ccggacacct
     4561 aacttgattc cggtgatgac gtcctctaat caataatgtc tatctcaaat tccggacttc
     4621 acgatagaat tactaaaatt cagttcttta aatttttcaa ttcgtacgaa tacctatatc
     4681 caagataaat ggtgtgtttg tctactatgg taaggccact acaaggtgat tcattatcaa
     4741 atcacacatc gcatatttca attcctcgtt tgaatgcctg taatttttcg tctttctcct
     4801 ttccctttga tataaattcc tgggcaacat agcctttaaa tccggtttca acaatagcct
     4861 tcatgatagc cgggtaatac aattcctgtg tatcgtctat ctcatttctc cccggtacac
     4921 ctgctgtatg ataatgtccg atatactgat gattgtcacg aatggtatgg atcacatctc
     4981 cttcatcgac ctgcatatga taaatgtcgt ataacagttt gaaattcggc gaaccgatac
     5041 gcttgcacag ctctacaccc catgccgtct tatcagccat atagtcatga tgatctactt
     5101 tgctattgag tagttccatc tggatgatga cattattctt ttcggccaaa ctcataattt
     5161 ttttcagtcc ttcctcacag ttttttaatc ctgtctcgtc atccataccc tttctattcc
     5221 cactgaaaca aatcaggttg gtataaccgg catctttgac catgggtatt gacttaatat
     5281 aatcctccac tagccgtgcg tgattctctc gctgagccca gccttctgtc aggcttactt
     5341 ttccatcgat atagcacatg gagcattcga ggttatattt ttttaaaata ttccaatcct
     5401 tggggctgat caggtctata gctcccacgc caatagattt aatccataat gccatctcct
     5461 ctatggttag aaaagagaca caccattgac agacactgtg tcgtatattg cccttccagg
     5521 ccagagattc ttcagagtca ttattaaaag cagacaactt ccctacgccg gtcaaagcca
     5581 gtcctccaag cgaaagcttt tttaatacct ctctccttcc atgaatattc tttttcacgc
     5641 tatatcttgt ttttcggatt ttttaaaaaa taataaaaat aaaacagtca caccggctgc
     5701 aaacattgcc gggaacaacc agaccttatc ccaatgatgg ttggtaacct ccccgccagc
     5761 atccaggatg acattagcac cggctatggc cccggctgcc cagaatccga tatacatccc
     5821 caaaccgtag gtagcaagtg tgatgagacc ttgagcggat gatctgactt gaggaccggc
     5881 tacctggtct gtgtatatat ttccggtaac aaagaaaaaa tcatagcaga ttccatgcaa
     5941 aatgattccg agatacaaca tccacagacc actatccaaa tcaccgtatg caaagcataa
     6001 ataccttacc acccaggcca acatgccaac caacagcata tacttgactc caagtcgtct
     6061 gaaaaaccaa ggcatcagga ataaaaacac agcttcgctc atctggccaa gactcatttt
     6121 ttgagcagca ccttgcatcc ctatttcatt gaggaagata ttagcctgac cataataaaa
     6181 tgccagggga atacaaataa gaatggatgc aatgaagaaa actgcataat tcttaatctt
     6241 caatactttc aacgcgtcca gaccgacaat ttccctgaaa gaaacatctt ttccggcttt
     6301 gggtggtggt gtgtcaggta gaaaaaaact atatatcccg aaaacaagag agatagcacc
     6361 ggcaatcttg aatgtcatca ccaggtcacc aggatttttc tccaatgcca aaaagccgac
     6421 aacctgaccg gctacaatcc acccgatggt tccccatagc cggataccac cgaattcttt
     6481 ctctgcatca ctcacctgat tcaatgcgat tgagttgcat aatgccagag taggcatata
     6541 tatcaccata taaatcaata acaatgtata aaaaccagtg aattccttag acccgcttat
     6601 aagatacatc aaaactgcac ctgcaagatg gagtatgcct aaaattttct gtgccgcaaa
     6661 atatttatca gcgatcaaac caatgataaa aggagctatg gttgccccca gacactgcgt
     6721 accaaatgca agccccacct gagcgtcaga agcttttata tcacccttga gtaaatatgt
     6781 tcccaacgtt acaaaccaaa ttccccaaac gaaaaagttg aggaacatca ttgagttaag
     6841 ttgaattctt acagttggct tcattaatag agattggtcc gtttagttaa attaaaattt
     6901 gtgtttcaaa taaataattg tggcctgaat ttcatttttg taaaagtaaa acctaaaagt
     6961 aatacaatta tccaaaattt taattttctt tatcatatca tttcagtata taaaattatt
     7021 atcaatatgt ttagaaaaat gagagtggca atgataggcg gaggtcgcaa tgcatttatc
     7081 ggcagcatac atcgtattgc cattcaaatg gatggattgg cggaaatcgt tgcaggtgca
     7141 ttgagtgcca atcctgaaaa tgccctggcg tccggcaagg atcttttctt accggaggac
     7201 agaatctata cggatttcag aaccatgatc aagaaagaat cggcatcgcc ttcagaaatc
     7261 cggcctgata tcatctgtat cgtgaccccc aatcatatgc actttgagcc ggccatgctc
     7321 gcactgcaaa atggatttca tgtagcaata gacaaaccaa ttacattcag taccgaagag
     7381 gcaatccgac ttaaagaggt attggagagc tccggaaaac agcttttact cacccatgta
     7441 tataccggat atccgatgat caaacaagcc agggaaatga tacaatctgg tgttttgggg
     7501 cgtatccgta aggtatttgt cgaatatccc caaggatggt taagtgctcc gcttgaacag
     7561 gaaaacaata aacaagcaag ctggagaact gacccgacaa aaagtggaaa agccggtgcc
     7621 atgggggata taggcaccca tgccttcaac ctggcggaat acgtatccgg cttgtctgtt
     7681 tataagattt gcgcagatgt aaatacaata gtacccggca gattactgga tgatgacgga
     7741 gctgtattat tgcgttttga agggggggcg agtggagtcc tgatggcaac tcaggtcgct
     7801 accggtgagg aaaacaatct acgtatcagg gtttatggag aaaagggtgg tttggaatgg
     7861 cagcaggcag attgtaacag tctcagggta atgtggccag acaaaccggc tgaaatattc
     7921 cggacaggag cagggtattc aggggcaatg gctgtacata atagtcgcac acctgccgga
     7981 catcccgagg gctacctcga ggcgtttgcc aatctttaca ggaatttctt tcatactatc
     8041 gctgcatcgg acaaacagga atctgaaaaa tacctggatt ttccgggcat agaagaaggc
     8101 attcgtggaa tgaaattcgt agataaagtt atcgaatcgg gagtgtcaga acagaaatgg
     8161 ctaacattct gatgctggaa taggaatcaa taaagaaatg ccgctaacca cctgaatctg
     8221 cctgttataa gcagtgtttg aggtaatccg gctctataga atacctgaga ttaaaaagaa
     8281 gggaagtctt gcgacttccc ttctttcaaa ttaaaccgaa aaagaacagg tttacatctt
     8341 aacgactcgg atagcctggt gtccaaattt tgtcacgatg cgtatcaaat acataccggg
     8401 aacaagataa cccgatgtct ccaaggggac tatattttta cctgcaatgg tatttacaag
     8461 ttgatcagaa acaactctgc cgttggcatc catgattgaa atggtagctt ccatggatga
     8521 acctgcctca aactgagcgg taaatacatc agaggaaggg ttaggataca ccatgtaatc
     8581 cgttatggta ttggtaatag cggatggaag attactgcca ccagaaatcg taactgtata
     8641 atcttctacc tctccatagc taaaagtttc gcaggaagtt ccatatgatc cgtacttcat
     8701 ttgaaccctc atccttgtag gcccattact ggctgtagtt ggcactgaga aacttctgtt
     8761 gatgttacct ttactgctag aagaaaagct ggtaaccttc tcacccggat ccagaaaatc
     8821 accatcccta ttataatcta tccaaacagt ccaatattca gtatagctgc cgttcattcc
     8881 ggcactcagg gtcattgtgt atgaacttcc ggcagataat gtagtattaa gggctgtaaa
     8941 atctccatat cccccattgc tacctgttac attattgata gaacctaact taaccaaatc
     9001 tatccattca taggatgacg tattgccttt tgacgtacaa taggaagctc cccctccggt
     9061 tgtggtaaaa gtaacagtag ccgaataatt acttgaaccg ttactgcaat ttgtccttac
     9121 tccgacatca taggaagtac cgggtgtcat attcgtcata ttgacaattg ttgaggatgt
     9181 ggtataggta ttccaggtac cggaagaggt tagcttatac ctgagtgtat aattgaccgc
     9241 tccactgaca ctattccaac taatggtagc tgaagtattt gttatattgg tggcagcaag
     9301 gcctgttgga acactacatc cgctaccccc tgacgtggtg aatgtaacaa tcgcactgta
     9361 ggctgaattt ccacccgaac aaatgcttct cacctgggtt tcatacgtag ttgatcctgc
     9421 caatccggtc agtgtaactg atgtggttgt ctgttgactt aatactgtcc acgaaccacc
     9481 ggttgctctg tactgtaatt gataggctgt tgctccggaa acagtgttcc atgacactac
     9541 tgcgcttgta tttgtaatat tagtgacaac cagcccggaa ggtgcattgc aagtaccacc
     9601 acctgagcaa gttccaaggc atgaagcggc attaacccgg gatctgacca aattgcccgg
     9661 tagcgttcca aaaccattgt taaaattgat cccataactt gtcagatgac aataactcat
     9721 tattgttccg ccattggttg gaggaggtcc gggagggcaa ccaccttcag ttgtataaca
     9781 attatccaag gctccaccag gccatgaaca actctgtgta tgtggagagc caagattatg
     9841 gcccatctca tgtgtcatga cttcaactgt ccagctatag gttggtactg tctgataagt
     9901 agcctggata ttgctgtaag cgtatttgta tgacgtacaa agagcatcaa cccatgctac
     9961 accaccaata ttttgtccac caagggctgc aagatgagca agatctccat taaaactcgg
    10021 ccgattattc ctgaactggg taagggcggt ataactattt gttttactat acgggtccga
    10081 tgaagtccaa acaaaggtag aagaaatttg ggtagatatc tgctcattag catataatgt
    10141 tgcaacatta ttatatacag cagaaatcca gttgttaaca ttcgtaactg agcccttgtt
    10201 ctggtataat gcataatcac attcaatata taccttgacg catttaccat cccgtacttc
    10261 tattgaatta ttcatatatt gccttacttg ttccgcataa ttttcaggct cttcagtgtt
    10321 acaaaaattc gggtttttga ccaataggtc acgatctgaa taaataagat atctgtccgc
    10381 atcctccatc ttattaaaag ttatatttcc caactcttct gtggatatca ttcctatgac
    10441 atcatcctca aaaaaagaaa ttgccactgt tgaagacaca tcaccttcga caattcccct
    10501 ataatacaac cccggagaat aatttacagc cttttcagga gaatcgctgg tataggtttt
    10561 aaagtcatcg gaaagcacat ctacacgtac cagctccagc tgaatattct gtgacaaata
    10621 aggtacctga atattaattg catccggccg ggtattcaaa aaggaattca atgcctcctt
    10681 cctgagcttc aaatatgaaa attgcgaaag catgtccgga tacttatccc ttacatcaag
    10741 acttagatct tcaacaaaca aatcaacttt tggaaaagct tttatggatt tggcatgttg
    10801 tacagctcgt gcaacaggtt tgacattgtc aattgcaact tgctgggaaa gcatatctga
    10861 aggaaataaa atacaaagaa ggaaacctat taccaacgag gagaccttaa atgaggaatt
    10921 gtttgatagc atagccatta aagtttaata ttaaaaaatc aaaatcttta agtgtggaat
    10981 gcttaataat ctatggtaga tgatattctc ctaaaaggta tcaaagattg ggttgattat
    11041 caaagtttgt taaaaaatcc tgaccaaaga tacaaacaaa atggatcaac cagccattat
    11101 tgtaatacca attgataaaa acaatacaaa atacaacttt tcagggatgc ggtatggcag
    11161 gatcaaccta ctaaaacagg gcaaatgaaa ggtcatcagc cgggtcagat aacccggtga
    11221 tcattacggt aaattcaaaa atcttacaat taccatgtca aaaatatcgg gtcacatcat
    11281 actcagatgg cgtgaatcaa atcaactcaa tctggactca aaataaatca tttgacattt
    11341 acccaaatcc tgcaaaaaac aaaattgttt ta
//
LOCUS       DAOQMC010000054        11284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2237_length_11284_cov_4.752160, whole genome shotgun sequence.
ACCESSION   DAOQMC010000054 DAOQMC010000000
VERSION     DAOQMC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2237_length_11284_cov_4.752160"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKV31_03570"
     CDS             complement(<1..248)
                     /locus_tag="PKV31_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB91965.1"
                     /translation="MKQLISIFIFLLASMTISAQSLELARQYFEDGEYEKAAAIYKSL
                     WNKNKGNTTYLDALTDCYMALKDYDTAASVMSSAIEQNP"
     gene            452..1327
                     /locus_tag="PKV31_03575"
     CDS             452..1327
                     /locus_tag="PKV31_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNB91966.1"
                     /translation="MIFSMTGFGASKKAFYDKILSVEIKSVNSKIIDIRIRSPFNLRE
                     KENILRKEVADTALRGKIEVTVELSSANSTPLGMINEELLKSYAMSIKPIAADLELDT
                     SGLIAGLLRIPEVLAGGNNELSEEDWLILEATLDDALDRFQAYKQEEGKAIENDLLAC
                     INTIEESLVKIRDFETERIDRIRERITNGLEEFMPTESIDKNRFEQELIYYIEKIDFS
                     EEKSRLSQHCKYFRETLELDEVSKGRTLNFISQEMGREINTLGAKAYQSDIQRLIVVM
                     KDELEKIKEQTANIV"
     gene            1331..1921
                     /gene="gmk"
                     /locus_tag="PKV31_03580"
     CDS             1331..1921
                     /gene="gmk"
                     /locus_tag="PKV31_03580"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNB91967.1"
                     /translation="MEFEGKMIVVTAPSGAGKTTIVKYLLEKMDNLDFSISATTRDKR
                     PYEVDGKDYFFITKEQFEELIAKKAFAEYEIVYEGQYYGTLAAEIERIWQNGKHIIFD
                     IDVQGALTLKRKYMESCLTIFIRPPSKELLIERLTNRQTESPEALKKRIDKAEKELGY
                     ESLFDFVLINDQLEVACFEAETVVHLFLNPEIALNE"
     gene            1914..2360
                     /locus_tag="PKV31_03585"
     CDS             1914..2360
                     /locus_tag="PKV31_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB91968.1"
                     /translation="MSDIIFTTKVLWSQIDANMHMRHSAYADLGAQARLEVMQDKGFT
                     SEMMRKYMIGPILFREELLYKKEIRPNETVGVTCKLSRCRQDGSRWSIRHEIFKENGE
                     LAAIINADGAWIDQIRRKLAPLPEDFATRLLTLDKTEDYVEEVVTG"
     gene            2450..3817
                     /locus_tag="PKV31_03590"
     CDS             2450..3817
                     /locus_tag="PKV31_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNB91969.1"
                     /translation="MKYFFLITSFSILSLTITVAQGDIVSDSTRKIASTIAVPIDIAY
                     IDIEKMLNTSVNGLIYQDDSYTDNDNDQFKIKVWKKNNITIKPNAANQLKIAVPLKVW
                     AEKGVGALGLISYQSTEFELILNFKTIFSITQDWVIRTVTTPDGYTWITKPVLKYKYA
                     DVPITPIVANVLDKEHAGFARTIDEQVTKALDMKPYALMVWNLMNTPFQISEEYETWL
                     SVKPTAVQMTPLTALKDRITGTIGLNVISETRIGVKPVIPPPAIKPPNLKPVKTVPDN
                     FMVETIAEISYDNATEIANKQFRGMKLDFLNGKKSVTIEDIQVGRDGQKMTLTTKLSG
                     NIKGNVIIEGAPYYDSLAQRLALKNVDFQLKSKNFFQRTAAWLFNGKIEGMIEKDYGV
                     PVGDMITSAKTSLLETLNSNPHPGVRMKGMVTAMKPTAVILNDTGITIPILTAGKVAV
                     TIVDF"
     gene            complement(3821..4513)
                     /locus_tag="PKV31_03595"
     CDS             complement(3821..4513)
                     /locus_tag="PKV31_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNB91970.1"
                     /translation="MKLFSLIIIFLFGMYQMSMESGKWKPLFDGKSTSGWHKYGGGPV
                     GEAWKVKDGLLYLDTLAKDGWKIKGGGDIVTDKDYENFHLKLEWKISKSGNSGIMFYV
                     HEDPNQYPNSYETGPEMQILDNEGHPDAKIHKHRAGDLYDLIPCSTETVKPYGRWNKV
                     DIIANRGDLTFKLNGKVVVHTKMWDQGWNDMVAASKFHQWPGFGTYRKGRICLQDHGN
                     MVWFRNIRIKEL"
     gene            complement(4567..5619)
                     /locus_tag="PKV31_03600"
     CDS             complement(4567..5619)
                     /locus_tag="PKV31_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNB91971.1"
                     /translation="MRTIKGPGVFLAQFIGDKEPFDSLEGICGWAAELGFVAVQMPTL
                     DKRFIDLQLAAESKDYADELKGRIASFGLEISELSTHLQGQLVAVHPAYDELFDGFAP
                     PQLRGKPKERMDWAIGQLKYAARASQNLGLTAHATFSGSLLWHTFHPWPQRPAGLVEE
                     GFKELARRWTPILNTFDECGVDVCYEIHPGEDLFDGATYEMFLEAVHDHPRACLLYDP
                     SHFVLQQLDYIRYIDLYHDRIKAFHVKDAEFNPTGRQGTFGGYQSWADRAGRYRSPGD
                     GQVDFRTIFSKLAQYDYPGWAVMEWECCIKHPEVGAREGAEFIRKHIIPVTDKAFDDF
                     ASTVKEESLNKRVLGI"
     gene            5909..8008
                     /locus_tag="PKV31_03605"
     CDS             5909..8008
                     /locus_tag="PKV31_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91972.1"
                     /translation="MADIHLSLGFPWWYTMMCILVGIVFAWLMYRRFDFDHPWAGWAR
                     PFMFLLRALSVAIICFLLLSPVLKHVSYEEVKPTIVIGIDQSQSVGYALGKKQSEVLE
                     TVNALSDRLKGRYQVDIQSIGNDVNNKLSNDFNKPSTNLNQFFQNINEGVEYQALGAV
                     VMLSDGIFNTGPSPLFESSGIKAPIYTVGVGDTIPARDLSIRNVIHNEIGFLGDISKV
                     QVDIQAFNLKGHSARINISQDGHAFQGKPIMINRDDFFQTIEFEIPLSKAGMSRYIVS
                     VTQLPGEATTANNSKEFYIDVLDSKLNIDIVASSPHPDISAIKSVIDNNKNYDLKIHY
                     LYQPVKLREKVDLLILYQVPTVNNFASSFENLWKSIQHRPLPKLFVLGGQSNFSRFNQ
                     IQTTLQLIGNQGSLNDVYPLLRKGFQAFAARDEWQGIISGFPPLQTPFGTFVAGPNAQ
                     VLMEQRIGRVETQYPLWLIGQEDKNRVGIIAGEGLWRWRLSESAKTGRTTTFDDIVGS
                     TIRLLATREDKRKFKVRTNERVYEISDNISLTGELYNDNYELVNTPEVKLSLTDAANK
                     EYKFTLDRTDNAYRLDIGQLPAGNYRYMARVLFQGKELTSGGDFSVKNNDKELFDLMA
                     DFGLLRQISEDSGGRFISVNELDKLADMIKSNDAIKPIIRSDKRTDPLINLKWIFGLL
                     AFLLSAEWALRRYLGRY"
     gene            8010..8882
                     /locus_tag="PKV31_03610"
     CDS             8010..8882
                     /locus_tag="PKV31_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNB91973.1"
                     /translation="MDFGRLDDISEVDFSLPEIDHFYFRNNGNNHLELLLGAPGWSEP
                     KWKGSIYPAKAKPSEFAAYYSRQFATIEFNTTYYQVPKPALMERWYDLTEPDFVFCPK
                     MFAGISQSNSFALGDSILPDFLLRMGMLKEKLGLIYMQLPQSFSFVKLESLKKFIRQW
                     RPGIPLAIEIRHESYFKSQNAPVLAEEMAMANISWMITDVAGRRDASHSYITAPFMAI
                     RFVANNDESDFTRIDNWVDRLVVWQTNGVELVYFFLHTKGQPPIVLSEYLAKKWKEKS
                     THDIKKPTVIDREK"
     gene            8942..>11284
                     /locus_tag="PKV31_03615"
     CDS             8942..>11284
                     /locus_tag="PKV31_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNB91974.1"
                     /translation="MKNIYLLCAMLASTVMMGQTKEYMAFQRTSPDHKYKFSEVFGDP
                     AKARFYKLDNGLTVILAENHLEPQIMALIATKAGSKNDPADHTGLAHYLEHMLFKGTD
                     KFGTKDYAKEKVELDRIDELYELYGKTTDEAERKAIYHRIDSVSNVASKYAVANEYDK
                     MMSDIGSRMTNAFTSFENTTYMESFPSNNLEKYLKVQQERFRNPVLRLFHTELEAVYE
                     EKNISLDNGDEKIFEAMFGSLFKKHPYGTQTTIGTIEHLKNPSLKEIRNYYNKYYVAG
                     NMAIILAGDLDPDATIAIVEKYFGNMPAKNAPGFAFQAEDDVDTTTKITVYSPDEEKV
                     AIGYRLPGALEKEAPIANLVSSILYNGKSGLIDKDLVIDQKILEGYAFTYLLKDHGIF
                     WMQGKPKDGQSLDQVKELFLEEINKLKRGEFDTTIIQGTVNNMKLDLIRGTEKATNTA
                     FLLHESFVTGRPWVDYLNDMNVMSKITKADIMRFAAKWFQNNYTVIYKKTGNSDVPKV
                     VKPEIHPVELNRDAQSDFLKSIVEAPVSEIQPRFLDFDKDVQKSTLKKGIPLWYVPNQ
                     QNQLFSVYYKYDYGTNANPKLDLAMNYLNYIGSTTRSNSQINKELYNLGLNWGVSVGQ
                     EEMNIYLTGLEENFDKGIEIIEDLLNNPKPDKAALAKLINNQIKARQDNLLNKETILY
                     SGLQNYLKYGAKNPFNSTLTNAQLRQITAEELVAIIKANAGLEHRIMYYGARPLNDLT
                     KSMAARHKTTSMLKPAPKGMEFTPAPSPENTVYFVDYDMVQ"
BASE COUNT         3442 a         2558 c         2463 g         2821 t
ORIGIN      
        1 gggttctgct caatggcact cgacatcacc gatgcagcag tatcataatc cttgagggcc
       61 atgtagcaat cagtcagcgc atccaggtag gtagtgttac ccttgttttt attccaaagg
      121 gatttgtaga ttgctgcagc tttttcatac tcaccgtctt caaagtattg gcgtgcaagc
      181 tcaaggctct gtgccgatat tgtcatggaa gcgagcagaa atatgaaaat agaaatgagt
      241 tgtttcatga tgaaatttaa tgggcaaatt tacagattcg ccatggttgt attgacaatt
      301 gccgaagtaa ataaaacgaa aagggacgcg aattgtttta tccggctctc ttcattcata
      361 aaattaacta agtaagcctt tgcaatgctt tttgtgccta gatttgccca aattttacta
      421 tttttgcgtt tctcaaaaaa tcgcatatcc tatgatattt tcaatgaccg gttttggtgc
      481 atccaaaaaa gccttctacg ataaaattct atcggttgaa atcaaatcgg tcaactcaaa
      541 aataatcgat attcgcatcc gttctccctt caaccttcgg gaaaaggaaa atatcctccg
      601 caaagaagtg gcagacacag cactacgtgg caagatcgaa gtgaccgttg aattatcatc
      661 cgccaacagt acgccgcttg gaatgatcaa cgaagagttg ctcaagagtt atgctatgtc
      721 catcaaaccc atcgccgctg atctggaatt agacacatcc ggcctcatcg ccggattgct
      781 ccgtattccg gaggtattgg caggaggcaa caacgaactg agtgaagaag attggcttat
      841 attggaggcc actctggatg atgctcttga caggtttcag gcatataagc aggaggaggg
      901 taaggccatc gaaaatgatc tattggcttg cattaacacc attgaagaga gtcttgtgaa
      961 gatccgggat tttgagaccg aacggataga cagaatccgg gaacggatca ccaatggtct
     1021 cgaagaattt atgccgacag aaagtatcga caagaatagg tttgagcagg aattgatcta
     1081 ttatattgag aaaattgatt tcagtgaaga aaaaagcaga ctttcgcaac attgtaaata
     1141 tttccgggag accttagaac tggacgaggt gagcaagggg cggacactca attttatcag
     1201 tcaggagatg ggccgcgaaa tcaatacttt aggagccaag gcctatcaaa gcgatatcca
     1261 gcgcctcatc gtagtcatga aggatgagct ggaaaaaatc aaggaacaaa ctgccaatat
     1321 tgtctgatcc atggagttcg aaggaaaaat gatcgtggta acagcccctt caggcgccgg
     1381 aaagacgacc atagtcaagt atctgctcga gaaaatggac aatctggact tttccatttc
     1441 ggcaaccacg agggacaaaa ggccatacga agtagatgga aaagattatt tcttcattac
     1501 aaaggaacag tttgaggaat tgattgccaa aaaagcattt gcggaatatg aaatagtgta
     1561 cgaaggccag tattatggca cgctggctgc cgagatagaa cgtatatggc agaacggcaa
     1621 gcacatcata ttcgacatcg atgtacaggg cgcactgaca ctcaaaagaa aatatatgga
     1681 gagctgcctc accatcttca tccgaccgcc aagtaaggaa ttacttatag aaaggctcac
     1741 caacaggcag acagaaagcc ctgaagcact gaaaaaaagg atcgacaagg cagagaagga
     1801 gttgggttat gaatccctgt ttgatttcgt actaatcaat gaccagttgg aagtggcatg
     1861 ctttgaggct gagactgtgg tccatttatt tttaaatccg gaaattgcat tgaatgagtg
     1921 atataatatt tactaccaag gtcctgtggt cacagatcga tgccaacatg cacatgaggc
     1981 actcggccta tgccgatctc ggcgctcagg cgaggcttga agtcatgcag gacaaaggat
     2041 ttacatcgga aatgatgcgg aaatacatga tcggaccgat tttgttccgc gaagaacttc
     2101 tctataaaaa ggaaatccgc cccaatgaaa cagtcggtgt gacctgcaaa ctgagccgtt
     2161 gccgccagga tggcagccga tggtcaatcc gacatgaaat attcaaggag aatggtgaac
     2221 tggcagccat catcaatgcg gatggtgcct ggatcgatca gatcagaaga aaattagctc
     2281 ccctgccgga agattttgcc acccgtctgt taacccttga caagacagag gattatgtgg
     2341 aagaggtggt gacaggataa ggtccatgtc ggaatttgaa caaaaaaaac tgcctgcaca
     2401 ttgaattttt agttgtggaa attcgtttaa tgaaaaattt tcattcgagg tgaaatattt
     2461 cttcctgatt acttcgtttt cgattctttc tctcactatt accgttgctc agggtgacat
     2521 cgtgagtgac agcacccgga aaatagcatc aacgattgct gtgcccatcg acatcgctta
     2581 cattgacatc gaaaagatgc tcaacacctc cgtcaacgga ttgatctatc aggatgattc
     2641 ctataccgac aatgacaatg accagtttaa aattaaggtc tggaagaaaa acaacatcac
     2701 cataaagcca aatgctgcca atcagttaaa gatagccgtc ccactcaagg tatgggcgga
     2761 aaaaggcgta ggagcattgg gcctgatcag ctaccaaagt acagaatttg aattgatcct
     2821 caacttcaaa accatatttt ccattacgca ggattgggtc attaggacgg ttacaacacc
     2881 ggatggatac acctggataa caaaacctgt acttaaatac aaatatgccg atgtccccat
     2941 cacccccatt gtcgctaatg tattggacaa ggagcatgca ggattcgcca gaaccatcga
     3001 tgaacaggtc acgaaggctc ttgatatgaa accctatgcc ctcatggtct ggaacctgat
     3061 gaatacgcca tttcagattt cagaagaata tgaaacctgg ctttccgtca agccaacagc
     3121 ggttcagatg acacctctca ctgcacttaa agacaggatt acaggcacaa tcggcctcaa
     3181 tgtaatctct gagaccagga tcggcgtcaa gcctgtcata cctccacccg ccatcaaacc
     3241 gcctaatcta aaaccggtca aaacagtgcc ggacaacttc atggtggaga cgatagccga
     3301 aatcagctat gataatgcca ccgaaatagc caataaacaa ttcaggggaa tgaaactgga
     3361 ttttctcaac ggtaagaaat cagtcaccat tgaggatatc caggtaggaa gggacgggca
     3421 aaaaatgact ttgacaacaa agctatccgg aaacatcaag ggtaatgtca tcatcgaagg
     3481 tgcaccatat tacgatagtc ttgcgcaaag acttgcactc aagaatgtcg attttcagct
     3541 gaaaagtaag aatttctttc agcgaaccgc tgcctggtta ttcaacggaa agattgaggg
     3601 catgatcgaa aaggattatg gtgtcccggt tggcgatatg attaccagcg caaaaacttc
     3661 cttgctggaa acactcaatt ccaatccaca tccgggagtc cgcatgaagg ggatggtaac
     3721 cgccatgaag cccactgcag tgattctcaa tgacaccggc atcaccatac cgatattgac
     3781 agccggcaaa gtagctgtta caattgtaga tttctgaaac ctataattcc ttgatcctga
     3841 tattgcggaa ccagaccatg ttaccgtggt cctgtaggca aatacgcccc tttcggtatg
     3901 tgccgaagcc gggccactga tggaacttac tggccgctac catatcattc catccttgat
     3961 cccacatctt agtatggacc accaccttgc catttaactt aaaggtcagg tcgccgcgat
     4021 tggcaatgat gtccactttg ttccaccggc cataaggctt gacagtttct gtactgcagg
     4081 gtataagatc gtagagatca ccggcacgat gcttgtgtat tttggcatcc ggatgccctt
     4141 cattgtccag aatctgcatt tcaggcccgg tttcatagga attcgggtat tgattgggat
     4201 cttcatgtac atagaacata atgccgctat tgccactttt agaaatcttc cactcgagct
     4261 tcaaatggaa attttcatag tccttatcgg taacgatatc accaccgccc ttgattttcc
     4321 agccatcttt agctaaagtg tccagataca gcaggccgtc cttgaccttc catgcctctc
     4381 caacgggacc gccaccgtat ttgtgccaac ccgaagtgga cttgccgtca aagagtggct
     4441 tccatttgcc gctctccatg ctcatttgat acatgccaaa aagaaagata atgatcaacg
     4501 agaataactt catttatata agatttattg gattaaaaat ccggtttacg gatgatgtaa
     4561 ttgcaatcat ataccgagaa ctcttttgtt gaggctttct tcctttacgg ttgaggcaaa
     4621 atcatcaaac gccttgtccg tcacaggaat gatatgcttc cggataaatt cagcgccttc
     4681 ccttgctccg acttcaggat gtttgataca acactcccac tccatcacag cccagcccgg
     4741 atagtcatac tgcgctaatt tgctgaatat cgtcctgaag tccacctggc catcccccgg
     4801 tgacctgtac cttcctgcac ggtccgccca tgactggtaa ccaccaaaag ttccctgacg
     4861 ccctgttgga ttaaactcgg catccttgac atggaatgct ttgatccgat catgatacaa
     4921 gtcaatgtac cgaatataat ccagttgctg caggacgaaa tgcgaagggt cgtaaagcag
     4981 acaggctcgc ggatggtcat gcaccgcttc aagaaacatc tcataagtgg ccccatcaaa
     5041 caaatcctct cccggatgta tttcataaca gacatccaca ccacattcat caaacgtgtt
     5101 taagatcgga gtccaacgtc ttgccagctc cttaaatccc tcttccacca atcctgcagg
     5161 acgctgaggc cacggatgaa aggtatgcca cagtaaagac ccactgaaag tggcatgtgc
     5221 tgtgagtccc agattttgag aagctctggc agcgtacttc aattgcccaa tcgcccaatc
     5281 catgcgctct ttcggcttac cccggagttg tggtggcgca aatccatcga acaattcatc
     5341 atatgcagga tgcacagcca ccaattgccc ctgaaggtgg gtagacaatt ccgatatttc
     5401 aaggccgaag gaagcaatcc ttcccttcaa ttcatcggca taatccttgc tctctgctgc
     5461 cagttgcaga tcgataaagc gtttgtccaa tgtcggcatt tgtacggcca caaaacccaa
     5521 ttctgcagcc cagccgcata ttccctccag actgtcaaat ggctccttgt ctccgataaa
     5581 ttgtgccaga aatacacccg gcccttttat tgtacgcata atatttgtat ctaaaaatcc
     5641 gagtgaatgt aaaaaaaatc ttgcaattac cgtataaaga atgagaatgg taaattccct
     5701 caacaacagg aaggaggcat gatggatgac attcactcaa tgataccatt cattctcttc
     5761 attatgattt ttcaaaaagt gcagggaggg agttaactgt acccaataac tgccttttga
     5821 accaattgac ccggaatccg ttatcctctt tatccaaatc caaaatataa atcctatttt
     5881 tggcgttttt taacagactg gaaatttaat ggcggacata catctttctc tcggctttcc
     5941 ctggtggtac acaatgatgt gtatactagt cggcattgtc ttcgcctggt tgatgtacag
     6001 gcgctttgat ttcgatcatc catgggcagg atgggccagg ccatttatgt ttttactcag
     6061 ggcattgtct gttgccatca tatgcttcct gctcctttct ccggttttga aacacgtcag
     6121 ctacgaagaa gtaaagccca ccattgtcat aggtatcgac cagtctcaat cagttggata
     6181 tgccttggga aaaaaacaat cggaggtgct tgaaacagtc aatgccttaa gtgaccggct
     6241 taaaggaaga tatcaggtcg acatacaatc cattggcaat gatgtaaata acaaattgag
     6301 caacgacttc aacaaaccat caaccaacct taatcaattt ttccaaaaca tcaacgaagg
     6361 ggttgaatac caggccctgg gtgcggtagt aatgttgtcc gatggcattt tcaatacagg
     6421 gccgagcccg ctgttcgaat cttcagggat taaagcaccg atctatacag ttggtgtggg
     6481 tgatacaatt ccggccaggg acctaagtat ccgtaatgtc atccataacg aaatcggttt
     6541 tctaggtgat atctccaagg tccaggtgga catacaggca tttaacctca aaggacattc
     6601 cgccaggatt aacatctcac aggatggaca tgcttttcaa ggcaaaccga taatgatcaa
     6661 tagggatgat ttcttccaaa ccatcgaatt tgaaattcca ttgagtaagg caggtatgtc
     6721 cagatatatc gtctcagtga cccaactgcc cggagaagca actacggcca ataacagcaa
     6781 ggaattttac atcgatgtac tggatagcaa gttaaacata gatatagtcg catcatctcc
     6841 acatccggac atctcagcga tcaaatccgt catcgacaat aacaagaatt acgatctgaa
     6901 aatccattac ctgtatcagc cggtcaagct cagggagaag gtagacctgc ttattctgta
     6961 ccaggtacct accgtgaata attttgcatc ctcttttgaa aacctttgga aaagtattca
     7021 gcacagacca ttgccaaaac tgtttgtttt gggtggacaa agcaattttt ccaggtttaa
     7081 ccagattcaa accaccctgc aactgatagg gaatcaaggc agcctcaatg atgtatatcc
     7141 attgcttcgc aagggcttcc aggcatttgc ggccagggac gaatggcagg gaattatatc
     7201 cggctttcca cccctgcaga ctcctttcgg taccttcgta gcggggccaa atgctcaggt
     7261 attgatggaa caaaggattg gcagggtgga gactcaatac ccactatggc tcatcggcca
     7321 ggaagataaa aacagagtag gcatcatagc cggagagggc ttgtggcgat ggcggttaag
     7381 cgagtcagcc aagaccggaa ggacaactac ctttgacgac atcgtaggca gcaccatccg
     7441 tttattggcg accagggaag acaagcgcaa gtttaaagtc agaaccaatg aaagagtgta
     7501 tgaaatcagt gacaacatct ctcttaccgg tgaattatac aatgacaact atgaattggt
     7561 caacaccccg gaagtaaaat tgagccttac cgatgcggcc aataaggaat ataaattcac
     7621 cctggatcgt accgataatg catacagact ggacattggc cagcttccgg ccggcaatta
     7681 ccgctatatg gcacgcgtct tgttccaagg gaaggaactc acctcaggag gagacttttc
     7741 ggtcaaaaat aacgacaagg aactctttga cctgatggca gatttcggtc ttctgaggca
     7801 gataagtgaa gattcgggtg gccgcttcat cagtgttaat gaattggata agctcgctga
     7861 tatgatcaaa tccaatgatg ctatcaaacc gattatccga agtgataaaa ggaccgatcc
     7921 tctcatcaac ctgaagtgga tatttggcct gctggccttt cttttatcag ccgagtgggc
     7981 actgagaaga tatctgggca gatactgata tggatttcgg cagattagac gatatttccg
     8041 aagttgactt ttcattgccg gaaattgacc atttctattt cagaaataat ggcaacaatc
     8101 atctcgaatt attactcggc gcaccgggct ggtccgaacc caaatggaaa ggatccatat
     8161 atcctgccaa ggcaaagccc agtgaattcg ctgcttatta cagccgccaa tttgccacaa
     8221 tagaattcaa tacaacatac tatcaggtgc ctaagccagc gttaatggag cgttggtacg
     8281 acctcacgga gcctgacttc gtgttttgtc cgaagatgtt tgccggtatc agccaaagca
     8341 attcgtttgc actcggcgac tccattcttc ctgactttct gttacggatg gggatgttga
     8401 aggaaaagct cggactcatc tatatgcagt taccacaaag tttttccttc gtcaagctgg
     8461 aatcattgaa aaaattcatc cgtcaatggc ggcccggcat accattagcc atagaaatcc
     8521 gtcacgaaag ttattttaag tcacagaacg ctcctgtcct ggcggaagaa atggccatgg
     8581 ccaacataag ctggatgatt accgatgttg cagggagaag ggatgcaagc catagttaca
     8641 tcacagcgcc attcatggct atcagatttg tcgccaataa tgatgaaagt gatttcacaa
     8701 gaatagacaa ctgggtagac agattggtgg tttggcaaac aaatggtgtg gagttagtgt
     8761 attttttcct gcataccaag ggacaaccgc caatcgttct atctgagtac ctggcaaaaa
     8821 aatggaagga gaaaagcaca catgatatta aaaaaccgac tgttattgat agggaaaaat
     8881 aatcataaat tcccgaaatt ttcgacttaa acattagctt tgtcatttaa aaaattcata
     8941 tatgaaaaat atttatttgc tatgtgccat gctggcctcc actgtaatga tgggccagac
     9001 caaagaatat atggctttcc aacggacttc tccggatcac aagtacaaat tttcagaagt
     9061 attcggtgat ccggccaaag ccagatttta caaattggac aacggcctca ccgtcattct
     9121 ggccgaaaat catctcgagc cacaaattat ggcgcttatc gccactaaag caggttcaaa
     9181 gaatgaccct gcagaccata caggcctggc acactacctg gaacatatgc tgtttaaggg
     9241 aacagacaag ttcggtacca aggattatgc aaaggaaaaa gtggaactcg acaggatcga
     9301 cgaattgtat gaattgtatg ggaaaacaac ggatgaagca gagcggaaag ctatctatca
     9361 ccgcatagac agcgtaagca atgtagcttc aaaatatgca gtagccaatg aatatgacaa
     9421 aatgatgtcg gatataggtt cgcggatgac caatgctttc acgagttttg agaatacaac
     9481 ctatatggag agctttccat ccaataatct ggaaaaatat ctgaaagtac aacaggaaag
     9541 gttccgcaat ccggttttga gattgttcca tactgagttg gaggcagtct atgaagaaaa
     9601 gaatatttca ctggacaatg gagatgaaaa gatcttcgaa gccatgtttg gatcattgtt
     9661 taagaagcat ccatacggca ctcagacaac catcggtacc atcgaacatc tgaaaaatcc
     9721 ttcgctcaaa gaaatcagga attattacaa caaatattac gtcgccggca acatggccat
     9781 catccttgca ggcgatctgg accccgatgc caccattgcc atagtggaaa agtattttgg
     9841 caatatgcca gccaaaaacg cccccggttt tgcatttcag gccgaagacg atgtggatac
     9901 tacaacaaaa attacagtct atagtccgga tgaagaaaaa gttgcaatcg gctaccgtct
     9961 ccccggagca ttggagaaag aagcccccat cgcaaatcta gtttcctcta tcctatataa
    10021 tggtaaaagt ggcctaatcg ataaggatct ggtcatcgac caaaaaattc tggaagggta
    10081 tgcgtttacc tatctgttga aggatcatgg aatattctgg atgcagggca aaccgaaaga
    10141 tggccaaagt cttgatcagg taaaagaact tttcctggag gaaatcaaca aattgaaaag
    10201 aggtgaattt gacaccacca tcatacaagg tactgtcaac aacatgaaac tggatctaat
    10261 ccgaggcact gagaaggcaa ctaatactgc cttcctgctg catgagtcct tcgtcacggg
    10321 ccgcccctgg gtcgattacc tcaacgatat gaatgtgatg tccaaaatca caaaggctga
    10381 tatcatgaga tttgcagcca aatggttcca aaacaactat actgttatct acaaaaagac
    10441 aggcaacagc gatgtaccaa aggtagtgaa gccggaaatc catccagtcg aactgaatcg
    10501 ggatgcacaa tccgactttt taaagtccat tgtagaagca cccgtatctg agatccagcc
    10561 ccgcttcctg gattttgata aggatgtaca gaaatctacc ttgaaaaagg gaataccgct
    10621 atggtatgtc cctaatcaac agaatcaatt attcagtgtc tattacaaat acgattacgg
    10681 caccaatgcc aatccaaagc tggatctggc catgaactat ctgaactata tcggctcgac
    10741 cacccgatcc aattcacaaa tcaacaagga attgtacaat ctggggttga actggggagt
    10801 cagtgttggc caggaagaaa tgaatatcta cctgaccggt cttgaagaaa attttgacaa
    10861 gggcatagaa attatcgagg accttttgaa taatccaaag ccggacaagg ctgcattggc
    10921 caaattgatc aataatcaaa tcaaggcaag gcaggataat ctgctcaaca aggaaaccat
    10981 cctctatagc ggactccaaa attatctgaa atatggagcc aaaaaccctt tcaacagcac
    11041 cttgaccaat gcgcaattac gtcagatcac tgccgaagaa ctggttgcca ttatcaaagc
    11101 caatgccggc ttagaacata gaatcatgta ttatggtgcc cgaccactca acgatttgac
    11161 gaaatcaatg gcagcacggc ataaaaccac atcgatgctt aagcctgcac caaaaggcat
    11221 ggaatttact ccggcaccat cgcccgaaaa tactgtttat tttgtggatt atgacatggt
    11281 tcag
//
LOCUS       DAOQMC010000055        11232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2250_length_11232_cov_4.611792, whole genome shotgun sequence.
ACCESSION   DAOQMC010000055 DAOQMC010000000
VERSION     DAOQMC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2250_length_11232_cov_4.611792"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKV31_03620"
     CDS             complement(<1..215)
                     /locus_tag="PKV31_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91975.1"
                     /translation="MRKYIVLITITAIVAALFVAKFLFFPAQEEQKMGFMAGGKKSGP
                     LGVDIQVLKSEKANGTLQSTGTILANE"
     gene            complement(300..710)
                     /gene="mce"
                     /locus_tag="PKV31_03625"
     CDS             complement(300..710)
                     /gene="mce"
                     /locus_tag="PKV31_03625"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNB91976.1"
                     /translation="MIRLEHVGIAVSNLKEANELFEKLFGRKAYKEEHVDSENVTTSF
                     FQLGESKIELVGSDPEVETAISKYISKKGQGIHHLAFEVDDILESMQEMREKGFILLN
                     EEPKPGADNKLICFLHPKSTNGVLVELCQSIKSR"
     gene            complement(790..2961)
                     /locus_tag="PKV31_03630"
     CDS             complement(790..2961)
                     /locus_tag="PKV31_03630"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNB91977.1"
                     /translation="MNSKYFTLLLLIFCAMNVSAQRSVKNVSTSVGQCVVSPINKSGE
                     TEKTHLVKIETKKNQPIIRGLRKLGLVEEFTNASCGPCAAQNPAFNTLIDANEGKIIA
                     VKYQVWFPGFDPMFETNKEEVQAKWSTYEDYTVGLYGSDNGLGGVPTAFLDGYMGNGN
                     YGGGNWNSAYYGAPVGYNQAAFNYAAAQVSPLDIQVSHSYNNTIDSVNITVIVTNNSD
                     TTFNISGYNLHAYLLESKLNWPVAPGSNGEKDFSHVFRKAASSIFGDNVLPASFASGE
                     SATFTYTVALKNYFFALNQIEALAFIQKTTDLSVMNAALSEPVSFPAGTTVGDPGVAN
                     SSKSSSEKCPAEYTAEPIIKITNQNPDKSAITQVTVAYSLNGEEVEKSFDTNIEFGKS
                     ENISFGSIPVPGGTNQIDVWIVSFNNGAKDVNLINNLTETYSFTYLSEHGAPAPKSYD
                     MEGDGALGKTLFLDDANFDYIFTMTPNLTNPPANRGGYGKSANSIILFFFQWNPASTI
                     ASMSFISDKINMPSNPVYTYDWAYTGYLENGVKYDGDKVEMFYSTDCGATYKKFKTLS
                     GNTMKTAPDKTALFLPTASEWRTDTVALSELANTDDVIFKTTVTSNWGNAGYLDNIFV
                     GQGATNTVKGINSFENVSVYPNPTADQLNVRINAKNPSTATVTILDYTGKTVLTLNNS
                     QTIFNGSNNLSFDVSSLSSGTYLLKIENKEGVNIQRFTVFE"
     gene            complement(3234..6647)
                     /locus_tag="PKV31_03635"
     CDS             complement(3234..6647)
                     /locus_tag="PKV31_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB91978.1"
                     /translation="MQDINKEFKPTTWSIKNKTSIYLATLFITILGIATYFSLPKENF
                     PEVVIPQIFVATVYAGTAPADMENVVTKVLEKELKAISGVKKVTSNSFQDYSTIIVEF
                     NTGIKIDVAKQKVKDAVDKAKPDLPNDLTKEPDVIDINLSDLPIMNINLSGDMDLLQL
                     KEYAEDVKDRLESIKEINRIEIVGALEREIQVNVDLFKMAAAGASFMDIENAIKYENM
                     TISGGEINMDGTKWAVSVKGEYRNPLLMNDLMVRTSSGGTIYLRDVATVLDATAEQES
                     YARLDGKNVITLNVIKRAGENLISASHSIKQELENMRGRVLPANIKATITQDQSIETE
                     ITLHDLINTIILGFLFVTFVLMFFMGTSNALFVGLSVPISAFLTFLVLQGAGYTMNMI
                     VLFSFLLGLGIVVDDAVVVIENTHRIFANGKVNIFKAAKMAAGEVFLPVLTGTVVTLT
                     PFIPLLFWPGIIGDFMYYLPFTLIIMLLASLLVAYLINPVFAVDFMKPHKSHKDTIAL
                     DKSFIVTSAAFVAAAIFGYLMNVGFGNLVVTVYVFFVLNKFVFSWLIDKFQTNVWPKF
                     QNVYYNIVKSSIKGKRPVWVFLSGVVLLILSVIFTGIRAPKVDFFPSADPNYVITYLR
                     MPIGTHQSVTDSITQVLEKRINTVLGENNPIVKSVIANVALGAGSNEDMQTQAMPHLG
                     KVTVAFVEYAKRNGKSTVEYLDQIREIVKGIPGTEIVVDKESNGPPTGKPINIEIAGD
                     NFDELIDNASKLKLYLESKQIPGIEELRSDFQSNKPEIIVNIDRRRANQEGISTAQIG
                     SDLRTAIFGKEISKYRDDKDEYKVQLRAQPDQRNNINTILNMPLTFRDMAMMGRVRQV
                     PVSSVAGVEYSNTYAGIKRINKKRVITLSSNVLTGYTANEVVEKIKGQIAKFNHPESI
                     SIKMTGEQEDQAETMSFLMRAFMIAFGMIFLVLVLQFNSTTKPLIILSEIIFSITGVL
                     LGFSLFNMNIVIVMTGVGLFALAGIIVRNGILLVEFTDLLLHQGMELRAAAAEAARTR
                     MTPVLLTAIAAILGLIPLAIGFNIDFVSLFTTGDPKIFLGGDNVAFWGPLSWTMIFGL
                     IFGTFLTLIMVPCMLVMVEKGKERIYRLFGREYSAEKAAMKKKDLDSLT"
     gene            complement(6699..7850)
                     /locus_tag="PKV31_03640"
     CDS             complement(6699..7850)
                     /locus_tag="PKV31_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB91979.1"
                     /translation="MKNNILLSMIILTSLSLVSCSNEGNSSKSLAAKQKALDKKIALR
                     SQLDKEIEALEKEIAALDTTSIKEVTKIVGITNINTGSFSSQVEVMGKVDVANNASLS
                     AAQGGIVTHIFVKEGDHVKTGQTLAQLDNDVMNKSLIQARQAVAFTTDLFNKQKALWD
                     QKIGSEVQYLNAKNNMESAQNQLNIALQQNELFKIKAIYSGVVDLVDIKKGQVVAPGM
                     PAFRIVATKGLKLIANVPESYIGKIKQGNTVQVYFPDLNKEITGKVDYLSRVIDPLSR
                     TINAEILLPDNQSEIKTNMVGILRIKDYNNANAITIPVKNLLKNAEGYYVFIAEKSGE
                     KLVAKPRQIKTGAANGDKIEVTEGLKAGDQLITTGFQSIHEGDVLIMSE"
     gene            complement(7878..9209)
                     /locus_tag="PKV31_03645"
     CDS             complement(7878..9209)
                     /locus_tag="PKV31_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB91980.1"
                     /translation="MKRLFTYLLLIACWTSSLPISAQQSFSLNQAVEYGIKNAAATKK
                     DLLKIADAEQQIKAVRASGFPQFNGSVEYQYFAEVPEMVIPKEFDPTGQGGTVSFQKS
                     NNLNLGINTGFVLFDGAYLVGLKAAKFYRELVHAQAEITPINIRKNITDSYYTVLIAM
                     KNKEQLEKNITVLQKMRGEAEQIYKNGFAEKMDIDRLDLSISNLQSTMNNLMRNIEIL
                     KNILKFQMNYPLEQEITLTDEFDVLFLQASKDDGKSIEQFNINKRPEYRAMGRGIELA
                     ALDVKRQQQGYLPSLSFYASFTRGLQSDNIFKPGGFWIPTTVIGLKLNVPIYDSGMKK
                     AVIQRARIVVEQNTLDRSEFERAGNIEVTNTQKAYLNSIETVKEKQKSLDLAEEIFKV
                     AQTKYKEGVGTSLEISQAETDLYGAQAALIQAQGDVINARFAVEKALGNFN"
     gene            complement(9231..9842)
                     /locus_tag="PKV31_03650"
     CDS             complement(9231..9842)
                     /locus_tag="PKV31_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB91981.1"
                     /translation="MDTKDLLGYKAFEIFVRYGFRAVTMDDLAKEMSISKKTLYQHFN
                     NKEDVIRQALNVYHKRQKTKMENIQKEAQNPIEVIFLVFKILIDESKDVNIMNFLDLK
                     KYYFDIWQNSANSNEEYFCNIISTNLERGMAIGFYRPDLNRDIIAKLFYHLALALTSQ
                     DNNLLNEYPLRDFIRQIVTYHLNGVCTDEGRTILENYIKQYFD"
     gene            complement(10296..10709)
                     /locus_tag="PKV31_03655"
     CDS             complement(10296..10709)
                     /locus_tag="PKV31_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91982.1"
                     /translation="MYDIPIERLIPQRPPFVLVDGIKEHETAVTKAHFLIPKDHILIE
                     HGKLSAYGLLENMAQAAALRSGYESLEKGENPKTGFIGTITNAIVHALPEAGTEVITT
                     VHQTNQILNFILIECTAKSGDLVLAECAMKIVLLD"
     gene            complement(10764..>11232)
                     /locus_tag="PKV31_03660"
     CDS             complement(10764..>11232)
                     /locus_tag="PKV31_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91983.1"
                     /translation="KDEVDVEWIGHPNWFFKISKYTMPFIRNNPYIPETRFLNTIQHI
                     PEDLENFVLKPLFSFSGEGVKFHVTKKDIEGVKDPENWILQEKVNYYPAIKTPNPDEP
                     VKFEIRMMHTWEQGAPAPLLVNNLIRLSKGDMVGVKYNKDKDWIGASIGFFEQ"
BASE COUNT         3084 a         2286 c         2201 g         3661 t
ORIGIN      
        1 ttttcgtttg ccagaattgt tcctgtagat tgtagtgttc cgttagcttt ttcagatttt
       61 aatacctgga tatctactcc aagcggcccg gatttctttc cacctgccat aaatcccatt
      121 ttttgctctt cctgagcagg gaaaaagaga aatttggcta caaataatgc ggcgacaatt
      181 gctgtaatgg ttattagaac gatgtattta cgcataaaaa acgcttattt cttatagttt
      241 gatttcgttg aatccgacca tgaaactgac aaaattaaaa aaataagttg accaaagaat
      301 tatctagact ttattgattg acatagctca accaaaacac cgttggttga cttgggatgt
      361 agaaaacaaa tcaatttgtt atcagcaccg ggctttggtt cttcattcag taatatgaat
      421 cccttttccc gcatctcctg catggattca aggatatcgt ccacctcaaa agccaggtga
      481 tgtatgcctt gtcccttttt cgaaatatat ttggaaattg cagtttcaac ttccggatct
      541 gagccaacca gttcgatttt gctttctcca agctgaaaga acgatgtggt cacattttca
      601 ctatcaacat gttcttcctt atatgctttc cggccaaata atttttcaaa aagttcgttt
      661 gcttctttga ggttacttac tgcgatacct acatgttcca gacggatcat aattttaatt
      721 ttattctaat cttaatataa aaaaggctgc ctctaatgag acagcctata ttttttcaga
      781 aaggtcctat tactcgaata ctgtaaatct ttgaatattg acgccttcct tgttttcaat
      841 tttcaatagg taagtgccac tgcttaaaga actgacatca aaactaagat tattggaacc
      901 gttaaagatg gtctgactgt tgttaagagt cagaactgtt tttcctgtat aatccaaaat
      961 agtaacagtt gctgtcgatg gatttttagc gttgattctg acattcaact gatcggcagt
     1021 tggattcgga tatacactca cattctcaaa gctattgatt cctttgacgg tattggttgc
     1081 tccttgacca acgaagatat tatccaggta tcctgcgtta ccccagttgg atgtaacagt
     1141 agtcttgaat ataacatcat ccgtattggc caattcagaa agagctacgg tatcagtcct
     1201 ccattcagag gctgtaggta aaaacagggc cgtcttatca ggagcggtct tcattgtatt
     1261 tccggataag gttttgaact ttttgtaagt agcgccacaa tcggttgaat aaaacatttc
     1321 aaccttgtca ccatcgtatt taactccatt ttcaagataa ccggtataag cccagtcata
     1381 ggtatacaca ggattggatg gcatattgat cttatcgcta ataaatgaca tagaagcgat
     1441 tgttgatgcc ggattccact ggaagaagaa aaggattatt gaattagctg atttgccata
     1501 tccacctctg ttagccggag ggttagtcag attgggggtc atagtaaaaa tatagtcaaa
     1561 attcgcatca tcgaggaaca gtgttttacc taatgcacca tcaccttcca tgtcataaga
     1621 tttgggagcc ggagcaccgt gttcactcaa atatgtgaaa gaataggttt ctgtaagatt
     1681 gttaattaga ttaacatctt tagcgccatt gttgaatgaa acaatccaca catcaatctg
     1741 gttggtaccg ccgggaaccg gaatagaacc aaagcttata ttttctgatt tgccaaattc
     1801 gatattagta tcaaaactct tctctacctc ttctccgttg aggctatagg caacagtaac
     1861 ttgtgtgatt gcagacttat ccggattctg attggttatt ttaataattg gttctgcagt
     1921 atattctgca ggacactttt cagaacttga tttagaactg ttcgcaacac ccggatcacc
     1981 tacagttgta ccggcaggga aggaaaccgg ctcgctcaat gcagcattca taacggatag
     2041 gtcggttgtc ttttggatga aggccaaagc ctctatttgg ttcagagcaa aaaagtaatt
     2101 tttcaaggca acggtatagg taaatgtagc agactctcca ctggcaaatg aagcaggaag
     2161 cacattgtct ccaaatatag aacttgccgc ttttctgaat acatgcgaga agtctttttc
     2221 tccgtttgat cccggagcaa ccggccagtt tagcttagat tccaacaaat atgcatgcag
     2281 attataacca ctaatattaa atgtagtatc actattgttt gttacgatta ctgttatatt
     2341 tacagaatca atggtgttgt tgtaactatg agaaacctgg atatctaaag gactgacctg
     2401 agctgcggca tagttaaatg cagcttgatt gtatccgacc ggtgcaccat aataggcaga
     2461 attccagtta cctcctccat agttaccatt acccatataa ccatctagga aggcagtcgg
     2521 aacacctccc aagccgttat ctgacccgta aagtccgact gtataatctt cataggtaga
     2581 ccatttggct tgaacttcct ctttgttagt ttcaaacatg gggtcgaaac cgggaaacca
     2641 aacctgatat tttactgcta tgattttacc ttcattggcg tcaataagag tattaaaagc
     2701 agggttctgt gcagcacatg gaccgcagga ggcattggta aactcttcaa ccaatccaag
     2761 cttgcgtaag cctctgatta ttggttggtt tttcttcgtt tcaattttaa ccagatgggt
     2821 cttttcggtt tctccggatt tattaattgg agatacaaca cattgaccaa cactggttga
     2881 tacgttctta acagatcttt gtgcgcttac attcatggcg caaaaaatca aaagaagtag
     2941 tgtaaaatac ttactgttca taaatgaata tcttattttg ttaaatgaaa gcgcaaaggt
     3001 acgaattaaa ctactattga cagaatataa atttaattat ttaagaaata tttatcattt
     3061 gcgaaatgat gtccccaggg caacaaatat tatttcgatg gagtctaatt tattcgtttg
     3121 aacaatattt tattttgaat ttgtataaat aaactttgaa gatcaaccta tgcccatgat
     3181 atgctgatgt aattttttta agaactggtc aataaatctc tttcgggaaa taattatgtt
     3241 aaagaatcaa ggtctttttt cttcatagcg gccttttctg ctgaatactc tcttccaaac
     3301 aaccgataaa tcctttcttt tcctttctct accatcacaa gcatacaagg taccataatc
     3361 agggtcagga aggttccaaa tatcaatccg aatatcatag tccagcttaa aggtccccaa
     3421 aatgctacat tatcacctcc caggaatatt tttggatcac ctgtggtaaa tagtgaaaca
     3481 aaatcaatat taaatcctat agccaatggt attaaaccaa gaattgccgc gatagcagtt
     3541 agaagtaccg gtgtcattct tgtccttgct gcttcggccg cagcagcacg cagctccatg
     3601 ccctgatgaa gcagcaaatc ggtaaattcc accaatagaa ttccattcct tacaatgatt
     3661 ccggctaatg caaaaagtcc cacccctgtc atgacgatga ctatattcat attgaataat
     3721 gagaatccaa ggagcacacc ggttatactg aaaatgattt cagagaggat gatcaagggc
     3781 ttagtggtag aattgaactg aagcacaagc accaggaata tcattccgaa ggcaatcatg
     3841 aatgcgcgca tcagaaaact catagtttca gcctggtctt cctgctcacc tgtcatttta
     3901 atagaaatgg actcaggatg attgaatttt gctatttgac ctttaatctt ttctacaact
     3961 tcattagcgg tatatcctgt aagaacattg gaggacaatg taatgaccct tttcttgttg
     4021 attcttttga tacctgcata ggtattggaa tattcaacac cggcgactga agaaaccggg
     4081 acttgtcgga ctcttcccat cattgccata tctctgaatg tcaagggcat attcaggatt
     4141 gtattgatat tgtttctctg atcgggttgc gcccttagtt gtactttata ttcatcctta
     4201 tcatccctgt actttgatat ttctttacca aatatggctg ttcggagatc agatccaatt
     4261 tgtgcagtag atattccttc ctggttagca cgtcgtctgt caatatttac gatgatttct
     4321 ggtttgttgg actggaagtc agatcttaac tcttcgatac ctggaatttg tttggactcg
     4381 agatataatt tcaactttga tgcattatcg atcaattcgt caaaattatc tcccgctatt
     4441 tcgatattga tcggtttgcc ggtaggtgga ccattgcttt ctttatctac cacaatctca
     4501 gtaccgggaa ttcctttcac aatctccctt atttgatcga gatattctac tgttgatttt
     4561 ccattgcgtt tagcatattc aacaaaagcc actgttactt tacctaaatg tggcatagcc
     4621 tgagtctgca tatcttcatt ggatcccgca ccgagtgcca cattggcaat tacggatttg
     4681 acgatcgggt tgttttcgcc cagtacggta tttatccttt tctccaaaac ctgggttatg
     4741 gaatcagtta cagattgatg cgtaccgatc ggcattctaa gataagtgat tacatagttg
     4801 gggtcagcac ttggaaaaaa atctactttt ggggcacgaa tacctgtgaa tataacggaa
     4861 agaatcaata acaccactcc tgacaaaaac acccagactg gtctcttgcc tttaatggaa
     4921 ctcttgacga tattataata gacgttttga aactttggcc aaacatttgt ctggaacttg
     4981 tcgatcagcc aggagaacac aaatttattc aatacaaaga atacatagac tgtgacgacc
     5041 aggttgccaa aaccgacatt catgagatat ccgaatattg cagcggcgac aaaagctgcg
     5101 ctggtgacta taaaactttt atcaagcgca atagtgtcct tgtggctctt gtggggtttc
     5161 atgaagtcaa cggcaaatac cggattgatg agataggcta ccaaaaggga tgccagaagc
     5221 ataataatga gcgtaaaagg caggtagtac ataaaatctc ctatgatgcc aggccaaaac
     5281 aataacggta tgaaaggggt cagggtaacc actgttccgg tcaaaacagg taagaatact
     5341 tctcccgcag ccattttagc agctttgaaa atatttacct tcccatttgc aaagatcctg
     5401 tgtgtgttct cgatcacaac aacggcatca tcaacaacaa ttccgagccc aagcaggaat
     5461 gaaaacaaaa caatcatgtt catggtatat cctgctccct gaagtacaag gaaggtaagg
     5521 aatgcagaaa tggggacact aagtcctaca aaaagggcat tgctggttcc catgaaaaac
     5581 attaagacaa aggtcacgaa gagaaagccc agaatgattg tatttataag atcgtgcagg
     5641 gtaatttctg tttcgatgga ctggtcctgg gttattgttg ccttgatgtt ggccggcaat
     5701 acccgccctc tcatgttttc cagttcttgc ttaatggagt gggaggcgga aataaggttc
     5761 tcaccggctc tttttattac attaagcgta atgacgtttt tgccgtccag gcgggcgtat
     5821 gattcttgtt cggctgtcgc atccaacaca gtggctacat cgcgcaaata aatggttcct
     5881 ccgctgcttg tcctcaccat aaggtcgttc atcaacagag gattgcgata ctctcccttt
     5941 acgcttacag cccatttggt accatccatg ttaatttctc ctccggagat ggtcatattc
     6001 tcatacttga tggcattctc tatatccatg aaggatgctc cggcagccgc catcttaaac
     6061 aaatccacat taacctggat ttcccgttca agagctccta caatttctat ccgattgatt
     6121 tccttgatgg attctaacct gtccttgaca tcttctgcat actccttcaa ttgtagcaag
     6181 tccatatctc ctgacaggtt gatgttcatg atgggcaggt ctgacaggtt gatatcgata
     6241 acatccggtt ctttggtcag atcattgggc agatcaggtt ttgccttatc tacagcatcc
     6301 ttgacctttt gtttggctac atctatcttg ataccggtat tgaattcgac gatgatggtt
     6361 gaataatcct gaaaagaatt tgatgtaacc ttctttactc cgctgattgc cttcaattct
     6421 ttttcgagaa ctttcgtcac tacattctcc atatcagccg gagctgtacc tgcatagaca
     6481 gtggcaacga atatctgggg aatgacaact tcggggaaat tttccttggg aaggctgaag
     6541 taggttgcta ttcccaagat ggttataaac aaagtcgcaa ggtaaatgga cgttttgttc
     6601 ttgatggacc aggtggtagg tttgaattcc ttatttatat cttgcatatc tgtatttcta
     6661 aatgatattg aaaagtttgc cggatgattg gagccggatt attcgctcat gatcaggaca
     6721 tcgccttcgt gaatactctg gaatccggtc gttatcaact ggtcaccggc tttcagccct
     6781 tcagttactt caattttatc tccattggca gcaccggtct ttatttgcct tggttttgcg
     6841 accaattttt ctccggattt ttccgcaata aagacatagt atccctctgc attcttcaac
     6901 aggttcttta ccggtatcgt aattgcattg gcattattgt agtctttgat tctgagtatc
     6961 cccaccatat tggtctttat ctccgactga ttatccggta agaggatttc cgcgttgatg
     7021 gtcctgctca atggatcgat gaccctgctt agatagtcaa cttttccggt tatttcttta
     7081 ttaagatccg ggaagtaaac ctggacagta ttgccttgtt ttatcttgcc aatatacgat
     7141 tcagggacat tggcaatcag tttgagacct ttggttgcga caatacggaa agcgggcatt
     7201 ccaggagcta ccacctggcc tttcttaata tcgactaagt ccaccacgcc tgagtatatt
     7261 gccttaatct tgaaaagttc attctgttgt agtgcaatat tgagttgatt ttgcgcgctt
     7321 tccatattgt ttttggcatt gaggtattgc acttcgctgc ctatcttttg gtcccacaat
     7381 gccttttgct tattaaaaag gtcggttgta aaagctactg cctgtcgcgc ttggatcagg
     7441 cttttgttca tgacatcatt atcaagctgg gccagggtct gacctgtctt aacatgatct
     7501 ccttccttaa caaatatgtg ggtgacgata cctccctgag cggctgacag gcttgcattg
     7561 ttggccacat ctaccttacc catgacttct acctgagagg aaaagcttcc tgtatttata
     7621 tttgtaattc ctacgatttt tgtaacttcc tttatcgaag tggtgtccag ggccgctatt
     7681 tctttttcca gggcttctat ctccttatcc agttgacttc tgagagcgat tttcttgtcc
     7741 agtgcctttt gctttgcagc cagggactta ctactgtttc cttcgttgct gcaggacacc
     7801 agggatagtg atgtcaagat gatcattgaa agcaggatat tatttttcat gttatatcgt
     7861 tttttcttta ttttgaatta gttaaagtta cccagggctt tttccactgc aaatcttgca
     7921 ttgatgacat cgccctgagc ctgaatcaat gcagcctgtg cgccgtagag atccgtttca
     7981 gcctgtgaga tttccaaact cgtacctact ccttccttgt attttgtttg ggcgaccttg
     8041 aaaatttctt ccgcgagatc tagagatttt tgtttctcct tgacggtttc gatggaattt
     8101 aaataagcct tttgtgtatt ggtaacctca atatttccgg cacgttcaaa ttctgagcga
     8161 tccagggtgt tttgttcaac cacgattcta gctcgttgga tgacagcctt cttcatccca
     8221 ctgtcatata taggcacatt gagtttgagt cctatgactg tggtagggat ccaaaatccg
     8281 cccggtttga atatattgtc ggattgcaat cctcgggtaa atgaggcata aaacgaaagg
     8341 ctgggaaggt atccttgttg ttgtcttttt acatcaagag ccgctaattc tattcctctg
     8401 cccattgctc tgtattccgg ccgtttgttg atgttgaatt gttcaatgga ttttccatca
     8461 tccttactcg cctgaaggaa taatacatca aactcatccg tcaatgtgat ttcctgttcc
     8521 agtggataat tcatctgaaa tttcaggata tttttcagga tttcaatatt tcgcatgagg
     8581 ttgttcatgg ttgactgaag gttgcttatg ctcaggtcca gcctgtcgat atccattttt
     8641 tctgcaaaac cgtttttata aatttgttct gcttcgccac gcattttctg taacacggta
     8701 atgttttttt ccagctgttc tttgtttttc atggcgatca gaacagtata ataggagtct
     8761 gtaatatttt tacgaatatt gatgggtgta atctcggcct gagcatggac caactctctg
     8821 taaaatttgg cagctttcag cccaaccagg taggcgccat cgaaaagtac aaagccggta
     8881 ttgatgccca gattaagatt gttgcttttc tggaatgata cagtaccgcc ctgtcctgta
     8941 gggtcaaatt cctttggtat gaccatttcc ggaacttctg caaaatattg gtattccaca
     9001 gaaccattaa attgcgggaa gccggaagct ctcactgcct tgatctgttg ttctgcgtca
     9061 gctattttca gtaaatcctt tttagttgct gcggcattct ttataccata ttccaccgcc
     9121 tgattgaggc taaatgattg ctgggcagag atgggcaggc tggatgtcca gcatgcgatg
     9181 agtaacagat atgtgaacaa tcgcttcata tttactgatg tattatgttg ttaatcaaaa
     9241 tattgcttta tgtaattttc caaaattgtc ctcccttcat cggtacatac accattgaga
     9301 tggtaggtta cgatttgtcg aataaaatcc cgcaaaggat attcgttgag aaggttgttg
     9361 tcctggctgg tcagcgccag tgccagatga tagaatagct ttgctatgat gtcccggttc
     9421 agatcaggtc tgtaaaagcc tatggccata ccgcgctcaa gatttgtgct tattatgtta
     9481 cagaaatact cttcattgct atttgcagaa ttttgccaga tatcaaaata gtattttttg
     9541 agatccagga aattcataat attgacatcc tttgattcgt caatgagaat tttaaaaacc
     9601 agaaagatta cttcaatagg attttgcgct tctttctgaa tattttccat tttggtcttc
     9661 tggcgtttgt gataaacatt cagtgcttgc ctgatcacat cttccttgtt gttgaaatgc
     9721 tgatatagcg ttttttttga gatagacatt tctttagcga ggtcatccat tgtgaccgcc
     9781 cgaaatccgt acctgacaaa aatttcaaat gctttatagc ctagtaagtc ctttgtatcc
     9841 attgtgaatc ttaaaactct acttttgatg tgcaaaaata aactattttg ataaaatgga
     9901 aacaaaaaaa caaaaaacgt ttcctgtgta taaaaaacta aatattcatc aatttataaa
     9961 atcatataac aaaaatatta gaaatacaga ataaaatatt tatgtaaaaa taaataattg
    10021 gtatatattt cgttctgtag tgttatgctt ttcgatgaaa taaataaata ttcagattaa
    10081 tcttcaatac tgactgatga ttattaatta ttttttcaaa taaaagggaa aaatatcaca
    10141 cgcgtattat cttgacaatg tcatttttgt tgattaaaga ggtcgtactt tgtcaaccgt
    10201 tcttacagaa tagctttatt aagttattgt attgtgctaa aagtataatt tcagcaggga
    10261 agagcttatt taaaatcggg caaggaagca aaagttcaat ccaaaaggac aattttcatg
    10321 gcacattcag ccaatacaag atctccggat tttgcggtgc actcaatcag gatgaagttc
    10381 aggatttgat tggtttgatg gacggttgta attacttcag taccggcttc aggcagagca
    10441 tgtactattg cattggttat ggtgccgata aatccggttt ttgggttttc tcccttttcc
    10501 aatgattcgt agccggaccg gagtgctgcc gcttgtgcca tgttttccag gagaccgtat
    10561 gcagaaagtt tcccatgctc aatcaaaata tgatctttgg ggatcagaaa gtgtgctttc
    10621 gtaaccgctg tttcatgctc ttttattcca tccactaata caaagggagg cctttgagga
    10681 atcaatcttt ctattggaat atcatacatt tcattgttaa tttgtagtag ctactattca
    10741 atctgctcag cctggcatgt aagctattgc tcaaagaagc cgatgctggc gcctatccaa
    10801 tccttgtctt tgttgtattt gactcctacc atatcacctt tgctcaatcg tatcaggttg
    10861 ttgaccaaaa gtggggcagg ggctccctgt tcccaagtat gcatcatacg gatttcaaac
    10921 ttcaccggtt cgtccggatt cggggtcttt attgcagggt aataatttac tttttcctga
    10981 agaatccaat tttcaggatc ttttacacct tcaatatctt ttttggttac atgaaacttg
    11041 accccttcgc cgctgaagga aaacaatggt ttaagaacaa aattttcaag atcttccggg
    11101 atgtgttgga tggtattcag aaatctggtt tcaggaatat aaggattatt tcttatgaaa
    11161 ggcatggtgt acttactgat cttgaaaaac cagttgggat ggccgatcca ttccacatcc
    11221 acttcatcct tg
//
LOCUS       DAOQMC010000056        11141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2275_length_11141_cov_5.054483, whole genome shotgun sequence.
ACCESSION   DAOQMC010000056 DAOQMC010000000
VERSION     DAOQMC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_11141_cov_5.054483"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3135)
                     /locus_tag="PKV31_03665"
     CDS             complement(<1..3135)
                     /locus_tag="PKV31_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNB91984.1"
                     /translation="MSEPKEKLKYKSNNVLTDNDTVIESNVLGKFFPAVPFQAKSAEK
                     NDFEEEINNETEAQENHSLNFSSAAPPDDRITNHLFPNIVRTDSGIQLKANSNYTTPL
                     QPKPNKTKISSYSAPAFQLKTVEPTQNDSKEDDTSSVQNTSHSNSLPKDVLGNMEQMM
                     GADFSGVNIHQNSTNAHNVGALAYTQGEDIHFAPGQFDPTSKSGKELIGHELTHVVQQ
                     RQGRVQPTTQAKGLPVNDDEGLEAEADEMGRQAAQMKANPDAPPAQMSLFGNEQERIR
                     TKTGIKINNPTFDESRMRQELPRTSFTGAGDPRLAQAMTVLYSGATEGAEVEQSLRTI
                     AEIRGLDVSTVNEQYNRAIAIRTAGRQYANPNGAANDANDPSPDLDLDRHPDFTGSLS
                     QLRFGSTLGDVFGIDPVFGALISPTGGLVGPGNDSVSGDANNPTVLHGTVHDAAGYLL
                     NAHGVGPGYNYLKRSWELDATNPLAGQTSGTAYWADRNPIAQATATLGTFGVNAVMDN
                     PSILLGMIHPGLAVPGLMNAGRDYENNFIRSAVEVGGGNWASRGVDSISNTLNAGWNA
                     ASNLWDGVTGVASDVWDGARGVTSDVIGGMRGVASDVWSGARGVASDVWDGARGVTSD
                     VIGGMRGVASDVWSGARGVASDVWDGARGLASGVMGGIGGALKDSGIGQPLTAPGLGG
                     IASGLGRALSRAASGVGGALRDAGIGQPLTAPGVGGIVGGIGRALSGVTGGIGGALRD
                     AGIGQPLTAPGVGDLFDGLDRLLSGTTGGNGGALRDAGIGQPLTAPGVGGIVGGIGRA
                     LSGVTGGIGGALRDAGIGQPLTAPGVGDLFDRLDRLLSGTTGGNGGALKDSGIGQPLT
                     APGVGDLFDRLDRLLSSTTGGNGGALKDSGIGQPLTASGVGGIVGGIGRALSGVTGGI
                     GGALRDAGIGQPLTAPGVGGIVGGIGRALSGVTGGIGGALRDAGIGQPLTAPGVGDLF
                     DGLDRLLSGTTGGNGGALRDAGIGQPLTAPGVGGIVGGIGRALSGVTGGIGGALRDAG
                     IGQPLTAPGVGDLFD"
     gene            complement(3843..4274)
                     /locus_tag="PKV31_03670"
     CDS             complement(3843..4274)
                     /locus_tag="PKV31_03670"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNB91985.1"
                     /translation="MRIAIINGPNLNLIGIREKEIYGDKNFNDFLTELQQCYPNVDFL
                     YYQSNVEGELINDIQETGFLVDGIILNAGAYTHTSLAIADAVAAVKAPVIEVHISNIS
                     AREPVRHDSHLTAKCVGIISGLGLDGYRLAVEWFVEREKRK"
     gene            complement(4276..6474)
                     /locus_tag="PKV31_03675"
     CDS             complement(4276..6474)
                     /locus_tag="PKV31_03675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNB91986.1"
                     /translation="MEVKEGYLTAALQKYFGFNKFKAQQKEIIESLLAGNDTFVLMPT
                     GGGKSLCYQLPALLMEGTAIIISPLIALMKNQVDSIRGYSSRDNVAHFLNSSLNKGQI
                     KQVKQDIDEGKTKLLYIAPETLTKEENLEYFHNSKISFVAVDEAHCISEWGHDFRPEY
                     RRIRTMIEAIGQHIPVIALTATATPKVRSDIIKILDMDHENTFVSSFNRDNLYYEIRP
                     KVSKEQTMKSMLQFIKSQKKISGIIYVQNRKTTEDLAEVLNVNGIKAAPYHAGLDAKS
                     RARTQDDFLMENVEVICATIAFGMGIDKPDVRFVIHYDIPKSIENYYQETGRAGRDGL
                     EGNCIAYFSVKDLTKLEKFLRDKPVAEREMGALLLQEVEAYAESSACRRKFLLHYFGE
                     EFDASDCHKMCDNCRNPKELKDVTEEMQLALEITKELNEEYNIKVLVDFLTGNATQQL
                     KLYKFTEYDSFGCGKDYDDNFWNSIFRQALLRNLMYKDIENYGLIKISETGQEFMKTP
                     TSIKIPLNQVFPEDTTPDDEGNGGTAALDETLVKLLMDVRKKESKRHNVPPFVIFTDP
                     SIEDMATQYPISIDDMAGITGVSRGKAERYGKPFIDVIAKYVEENDIERPMDFLVKQV
                     ANKSKIKVNIIQSIDKKIPLEDIARANNLDRDEFYQELYGIVTSGTKLDLNYYTEDLI
                     DEYVKEDIFDYFMNSETDNLEDASKKLKELDITWEEIELMRIVFLSEMAN"
     gene            complement(7202..8233)
                     /gene="recA"
                     /locus_tag="PKV31_03680"
     CDS             complement(7202..8233)
                     /gene="recA"
                     /locus_tag="PKV31_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNB91987.1"
                     /translation="MSTEKEAKLKALQLTLDKLEKTYGKGTIMKLGDKPEVDVDAIST
                     GSLGLDIALGTGGFPKGRVIEIYGPESSGKTTLAIHAIAESQKKGGTAAFIDAEHAFD
                     KSYAERLGVDTTNLYISQPDNGEQALEIAENLIRSGAIDIIVIDSVAALVPKSEIEGE
                     MGDSKMGLQARLMSQALRKLTATIGRTGCCCIFINQLREKIGVMFGNPETTTGGNALK
                     FYASQRLDIRRSGSPIKDKDGNLLGNQVKVKVVKNKLAPPFRVAVFDIMYGEGISKSG
                     EIVDLGVDLDIIQKSGSWYAYENTKIAQGRDAAKQFIEDNPGVAEEIEAKIKDKIVAK
                     GGTASIEMD"
     gene            9313..10659
                     /gene="mtaB"
                     /locus_tag="PKV31_03685"
     CDS             9313..10659
                     /gene="mtaB"
                     /locus_tag="PKV31_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNB91988.1"
                     /translation="MATPRSVAFYTLGCKLNYSESSSISRMFENNGYSVNEFHEGADI
                     FVINTCSVTDSADKKCRNIVRQALRISPQAKVVVIGCYAQLKPEEITRIEGVDLVLGA
                     AEKFRIIEFVESIGNAPSKGMMMAGDVREANQFVSSFSMGDRTRSFLKVQDGCDYKCS
                     FCTIPMARGASRSDTTAQVIENARYIASQGIKEIVLTGVNLGDFGNGTEVIEGISHKK
                     NELFIDLIKKLDEVEGIDRFRISSIEPNLLTNEIIEFVAQSKRFVPHFHIPLQSGNNK
                     ILAAMRRRYKCELYADRVRYIKELMPHCCIGVDVIVGFPGETDEDFWETVRFIQNLDI
                     SYLHVFTYSERANTPAAEMGQVPVHIRRQRNDVLGQLSEVKRKLFYERHQGETRSMLV
                     EHGHSNMELTGYTDNYIKVSLPYDETLINQVVSVELGAFDVAGNLVGHIVSSEIIV"
     gene            10662..>11141
                     /locus_tag="PKV31_03690"
     CDS             10662..>11141
                     /locus_tag="PKV31_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HNB91989.1"
                     /translation="MCGRASLTKNESDLEESFGASFYTEDIARYIPLPAFNIGPVQKL
                     PFIAQDDPLHFQIGWWGWNVTIGNNTQLVINARLEEIDKKRTFVPHLNHGRCIIPLDG
                     YYEWQKCGSQKIPYRITVPDGGLFAVAGLWREEVANDGTVSKRIVIITRAAQKDIAGI
                     "
BASE COUNT         2992 a         2397 c         2370 g         3382 t
ORIGIN      
        1 gtcaaataaa tctcccacgc cgggtgctgt caatggttgt cctatgcctg catccctcaa
       61 agcgccacca atgccaccgg ttacaccaga caaggctcta cctataccgc caactatacc
      121 acccacgccg ggtgctgtca ggggttgtcc tatgcctgca tccctcaaag cgccaccatt
      181 gccaccggtt gtaccagaca agagtctatc taaaccgtca aataaatctc ccacgccggg
      241 tgctgtcaat ggttgtccta tgcctgcatc cctcaaagcg ccaccaatgc caccggttac
      301 accagacaag gctctaccta taccgccaac tataccaccc acgccgggtg ctgtcagggg
      361 ttgtcctatg cctgcatccc tcaaagcgcc accaatgcca ccggtcacac cagacaaggc
      421 tctacctata ccgccaacta tacctcccac gccggacgct gtcagtggct gtccgatgcc
      481 cgaatccttc aaagcgccac cattgccacc ggttgtacta gacaagagtc tatctaaacg
      541 gtcaaataaa tctcccacgc cgggtgctgt caggggttgt ccgatgccgg aatccttcaa
      601 agcgccacca ttgccaccgg ttgtaccaga caagagtcta tctaaacggt caaataaatc
      661 tcccacgccg ggtgctgtca atggttgtcc tatgcctgca tccctcaaag cgccaccaat
      721 gccaccggtt acaccagaca aggctctacc tataccgcca actataccac ccacgccggg
      781 tgctgtcagg ggttgtccta tgcctgcatc cctcaaagcg ccaccattgc caccggttgt
      841 accagacaag agtctatcta aaccgtcaaa taaatctccc acgccgggtg ctgtcaatgg
      901 ttgtcctatg cctgcatccc tcaaagcgcc accaatgcca ccggttacac cagacaaggc
      961 tctacctata ccgccaacta taccacccac gccgggtgct gtcaggggtt gtcctatgcc
     1021 tgcatccctc aaagcgccac caacgccact ggctgcacga gacaaggctc tacctaaacc
     1081 gctagctata ccacctaatc cgggtgctgt cagtggttgc ccgatgccgg aatccttcaa
     1141 agcgccacca atacctccca ttaccccaga ggctaaaccc cttgcaccgt cccaaacatc
     1201 agaagctacc cccctggcgc cactccaaac atcagaagct acacccctca tgccaccgat
     1261 aacatcagag gtaacacctc ttgcaccgtc ccaaacatcg gaagctactc ccctggcgcc
     1321 actccaaaca tcagaagcca ctcctctcat gccaccgata acatcagaag taacacctct
     1381 ggcgccatcc caaacatcgg aggcgactcc agtaacacca tcccataagt tcgatgcggc
     1441 attccatcca gcattcaagg tattggatat gctgtcaacg cccctggatg cccaattgcc
     1501 gccaccaact tcaacagccg atctaataaa gttattttca taatctctgc ctgcattcat
     1561 taatccgggt actgctaacc cgggatgaat catccctagc agaatagatg ggttatccat
     1621 gactgcattt actccaaatg ttcctaatgt cgcagtagct tgagcaattg gatttctatc
     1681 ggcccaatag gcagttccgg aagtctgacc ggctaaggga ttagttgcat ctaattccca
     1741 ggatcttttg aggtagttat atccggggcc cacgccatga gcatttaata aatagccggc
     1801 agcatcatga acagtgccat gaaggacggt cgggttgttg gcatcgccag aaacagaatc
     1861 attgccagga ccgaccaaac cacctgttgg gctgatcaaa gctccaaaaa caggatctat
     1921 cccaaaaaca tcgcccaaag tactgccaaa tctcagttga gaaagtgacc cggtgaagtc
     1981 agggtgtcta tctaaatcca gatcgggaga tgggtcatta gcgtcattgg cagcaccgtt
     2041 tggattggca tattgacgac cagctgtcct gatagctatg gcacgattgt attgctcgtt
     2101 gacagtagaa acgtctaatc ctctgatttc agcaattgtc ctgagtgatt gttccacctc
     2161 agcaccctca gtcgcacctg aatagagaac agtcatggct tgcgccaacc ttggatcacc
     2221 tgcacctgtg aaggaagttc ttggcaattc ctgtcgcatc cgactttcgt caaatgttgg
     2281 gttattgatt ttaattccgg ttttagtcct tattctttcc tgctcattgc caaataaact
     2341 catttgggca ggaggtgcgt caggatttgc tttcatttgt gctgcctggc gtcccatttc
     2401 atctgcctcc gcttccagac cttcgtcatc atttacagga agacccttag cttgggtagt
     2461 gggctggaca cgtccctggc gctgttgtac gacatgtgtc aattcgtgtc caatcaattc
     2521 ctttcctgat ttcgaagttg gatcaaactg gccgggagca aagtggatat cttcaccttg
     2581 ggtataagct agtgctccta cattatgagc attggtagaa ttttgatgga tattaactcc
     2641 tgaaaaatcg gcacccatca tttgttccat attgcccaga acatccttcg gtaggctgtt
     2701 tgaatgagaa gtattctgta cagaggaagt atcatcttcc ttactgtcat tttgcgttgg
     2761 ctctacggtt ttaagctgga aagccggggc agaataactg ctgatttttg ttttattggg
     2821 ttttggctga aggggagttg tataatttga attggccttt agttgaatac cggaatctgt
     2881 tcttactata ttcggaaata agtggttggt aattctgtca tcaggcggtg ctgcggatga
     2941 gaaatttaaa gaatggtttt cctgcgcttc agtttcgtta tttatttctt cttcgaaatc
     3001 atttttctct gccgattttg cttgaaatgg tactgcagga aagaatttac ccaaaacatt
     3061 tgactcaatg actgtatcat tatctgtaag tacattattg gatttgtact tcaacttttc
     3121 cttgggttca gacatgatgt tgttttaaat tgattgggtg tgtaaaaata gtgataaatc
     3181 cggataattc aaaaaaaaac gacttaatat agattggaaa aaataattca aaagtcatct
     3241 tgatgtcaag accgatgaca atattggtaa gccaggccat tttgcgtatt tgggttttca
     3301 agataagaaa atccacatcc tcattgcggg cctagatatt aagctatgaa tatacttctg
     3361 gataagcccg aaacacagag aatgattaat tttttatgtc ttgtagaagt tttaccttgg
     3421 ttttagacct ccaaatcggc taaaattgtg gattactttc gccggaatat tcagttattg
     3481 ggaaatttaa aaatactatt caagtttcaa gggtgccttg atgctggtaa atagtaaaaa
     3541 accagaaggt catcttctat tttgtttaaa tatgagatac gtatcttggt tttttagttt
     3601 gaatatcaat tttgcaaagt ctcggtttac aaattttgaa aatatggtgg ggtgtcttta
     3661 attcagctag aaaactttaa attgtttgta cctcaatatc cattggctta taaagcagga
     3721 ttaagttagc aagaaatgag atttttttta ctttgaatag attttcaaag ttaaaaaaaa
     3781 tgaaacttac ataatcaaat cgactgatta tactattttg gaaaaaataa aaaaggcaaa
     3841 atttattttc ttttttctct ctccacaaac cattcaactg ccagccggta accatccagt
     3901 cccagtccgc tgatgatgcc tacacatttt gccgtgagat gtgaatcatg acgcaccggt
     3961 tcacgtgctg atatgttgga aatatgtacc tcgatgactg gagccttcac cgctgccact
     4021 gcgtcggcaa tggcgaggga ggtatgtgta taagcacctg catttaggat tatgccatct
     4081 acaagaaagc ctgtctcctg gatgtcattg atgagttcgc cttcgacatt ggactgataa
     4141 tagaggaaat ctacattggg gtagcattgt tgcaattcag tcagaaaatc attaaaattc
     4201 ttgtctccat aaatttcctt ttcccggata ccgatcaggt tgagattcgg tccgttgatg
     4261 atggctatac gcatatcaat tggccatttc tgaaaggaag acaatacgca tcagctctat
     4321 ttcctcccag gttatgtcaa gctctttcag ttttttggag gcatcttcca ggttgtcagt
     4381 ttcgctgttc atgaaataat caaagatatc ttccttgacg tattcatcga tgagatcttc
     4441 ggtataataa ttcaggtcta gcttggtccc ggaggttaca atgccataca attcctggta
     4501 gaactcgtcc cggtcgaggt tgttggcacg agcgatatct tccagcggta ttttcttatc
     4561 tatgctttgg atgatgttta cttttatctt ggatttgtta gccacctgct tgacaaggaa
     4621 gtccatcggt ctttcgatgt cattttcttc gacgtatttg gctatcacat caataaatgg
     4681 cttgccgtat cgctcagcct tacctcggct gacaccggtt atcccggcca tatcgtcaat
     4741 agatatcgga tattgtgttg ccatatcttc gatcgaaggg tcagtaaata tcacgaaggg
     4801 aggtacatta tgacgtttgg attctttctt tctgacatcc atcagcagct tgaccagggt
     4861 ttcatccaaa gctgcagttc ctccgttgcc ttcatcatca ggggttgtat cttccggaaa
     4921 tacctgattc agtggaattt ttatcgaagt tggcgttttc atgaattcct ggcctgtttc
     4981 ggaaattttt atcaaaccgt aattctcaat atccttgtac attaggttgc gcaataaggc
     5041 ttgcctgaag atggagttcc agaaattgtc gtcataatcc tttccgcaac caaatgaatc
     5101 gtactctgta aatttgtata atttgagttg ttgggtagcg tttcccgtga ggaagtctac
     5161 taaaaccttg atattatatt cctcgtttag ctctttggta atctcaagtg ccagctgcat
     5221 ttcttcggta acatccttga gttccttagg attccgacag ttgtcacaca ttttgtgaca
     5281 gtcggaagca tcaaactctt cgccaaaata gtgcaggaga aatttacgtc ggcaggcgga
     5341 actttctgcg taagcttcta cctcctgaag caagagtgcc cccatttccc tttctgcaac
     5401 cggtttgtct cttaggaatt tttccagctt tgtcaggtcc ttgacagaaa aataagcgat
     5461 gcagtttcct tccagcccat ccctgcctgc tcgtccggtt tcctgatagt aattttcaat
     5521 gcttttgggt atgtcataat gaatgacgaa tcgtacatcc ggtttgtcaa tccccatacc
     5581 gaaagcgatg gttgcacaga ttacttccac attttccatc aggaagtcat cctgagtgcg
     5641 ggcgcgtgat ttggcatcca ggccggcgtg atatggggcc gccttgatac cgttgacatt
     5701 gagcacttca gcaaggtctt cagtggtctt ccggttttga acataaataa tacctgaaat
     5761 ctttttttga gacttgatga actgcagcat gcttttcatc gtttgctcct tagatacctt
     5821 gggccttatt tcatagtaca agttgtctct gttaaaagaa gatacgaagg tattctcatg
     5881 atccatatcg aggatcttga taatatccga acgaacttta ggagttgcgg tagctgtcag
     5941 ggcgatgacg gggatgtgtt ggccgatggc ctctatcata gttctgatac ggcggtattc
     6001 aggtcggaaa tcatgtcccc attcggagat acagtgtgcc tcatcaacag cgacgaagga
     6061 tatttttgaa ttatggaagt attctaaatt ttcttctttg gtgagagtct ccggagcgat
     6121 gtataagagt tttgttttgc cttcatcgat atcctgtttg acctgtttga tctgaccttt
     6181 attcagtgaa ctattgagaa aatgagcgac attatcccgg ctgctataac ccctaatgct
     6241 gtccacctga tttttcatca gagctatcaa tggtgatatg atgatggctg ttccttccat
     6301 taataaggca ggaagctgat agcaaagaga tttaccgcct ccggtaggca tgaggacaaa
     6361 agtgtcattg cctgccagca gactttctat gatttccttc tgttgtgcct tgaatttatt
     6421 gaaaccgaag tacttctgta atgctgcggt taaataacct tctttaactt ccataattca
     6481 ttacggatat gctattttaa taaataaaat ctatgatcac tgtttgactt aatcaaaatg
     6541 ccttttagaa aaagaaacaa aatcattctg ttttgcctca aatttgaatt ccatcttaat
     6601 tctttccagt taccaatttt tatttgagat tatcaaacca agataaggat ttttttaatt
     6661 taaaaagcaa ataattttat gagaatttta accttggatt aattagattt tcatttaatc
     6721 gtacattgac atgcaacacg ttgttttatt gatttgtata aatatatatt cagattatat
     6781 tatccgaaga taacccaaca aatcgccttg ggatgatggt agtaaatgca catttataaa
     6841 tatatgagat tgtgaaggat tgggtcattt gatatgtatt attggttgat tctccgataa
     6901 attgtattaa gcattgacaa tcaattggaa ggtatcggct ggaaatttgg caataagctg
     6961 agcaaatcat caaccctaat gcatcagttc ggaataagtg ataccaggat gatttatata
     7021 aaagtcctta aatttttggt atagtgccga ggttacagtc gtacagatca atgaatttgg
     7081 tctataacga atgtaccttc ggtgttaaat aatggtatta aacaaatgaa tggaagtaaa
     7141 aaaaaacagg cggttggagg tgatatctca accgcctgca aatgttgttt tcaggggatt
     7201 tttaatccat ctcaatggat gcagtaccgc ccttagctac gatcttgtcc ttgattttag
     7261 cttctatttc ttcagccact cccgggttat cctcaataaa ctgcttagca gcatctcgtc
     7321 cctgggctat tttggtattt tcgtaagcat accagcttcc tgatttctgt atgatatcca
     7381 gatccactcc caggtcaact atttcacctg atttggagat gccttcgccg tacattatat
     7441 cgaagactgc tacacggaaa ggcggtgcca atttgttttt gacgactttg acttttacct
     7501 ggttgccgag gaggtttcca tctttatctt ttattggaga gccacttctt cggatatcca
     7561 gacgttggga ggcatagaac ttaagagcgt ttcctccggt tgtcgtttcc ggattgccaa
     7621 acataacacc tatcttttca cggagttggt tgataaagat acagcaacac cctgttctgc
     7681 ctatcgtagc agtaagtttt ctaagagctt gagacatcag gcgtgcctgg agccccatct
     7741 tggaatcacc catctcacct tcaatttcac ttttaggtac cagggctgca actgagtcga
     7801 tgacaatgat gtctattgca cctgaccgaa taagattttc tgctatttca agcgcttgtt
     7861 caccattgtc aggttgcgaa atgtacaggt tggttgtatc tactcccagg cgttcggcat
     7921 atgatttgtc gaaggcatgt tcagcatcta tgaaggctgc cgtaccgcct tttttctgac
     7981 tttcggcaat ggcatgtatg gcgagtgttg tcttaccgga agattcaggt ccataaattt
     8041 ctataacacg acctttagga aatcctcccg tacccagggc gatatcgagg ccaagagaac
     8101 ctgtggagat ggcatctaca tcgacttcag gcttgtctcc aagcttcatg atggtgcctt
     8161 tgccataagt tttttcgagc ttgtccaggg ttaactgaag ggcttttaat ttcgcttctt
     8221 tttctgttga cataagattt tgatttaaaa aattaaagaa tgatttgaaa tcccgggtga
     8281 gttaaacatc ctaaaaaatg attaatcccc aagaatctgc aaatatattg aaataataat
     8341 aaattataat ctattaaatg tttatttttt cgtcattttt tgacgaaaaa atcagaatgc
     8401 tgcagaaatt tacttttctg atatagttat ttatcgatcc gagtattgcc ggagatggtc
     8461 gtaccacaat atttggttga tgacttattt tagtcaatgc atataatttt gcttttattg
     8521 ggctattatg aatcgagtat agagattttg aaattttctt caagcttgaa atccggtgtg
     8581 tcattttatt tagttgccaa gtaagttggt ataaaaaggt tggaatgagg gggcttggga
     8641 agtaggtagg aaaataataa tcatcagtca agccaatgaa gggtctattt tataatgtat
     8701 gattattttg ttttcattat cggtattaaa ttcaattcag gtggttaaag tatccgtgaa
     8761 tattgattgg atcactatta atcttctacc tacttagaaa ttgcataaca aaactatttt
     8821 ttgctcttaa cccaaggagc tttttgattt aaatgctgaa ttgaagaggg atgatttctt
     8881 aaaaattcga aataggtagt tgtttaatag tcgtctattc agttattatc aataatgatg
     8941 agatttcatt ctggaaaatt ttgattgaaa gccgataatt tgaataggca tactgtcctt
     9001 gcatgtatca cgtccgattt catagttatt aatcatctac ctacttgaat tttagatata
     9061 aataccaatc cggtcgtcaa tcctttacat gtattgtcac agttgcttga aggccccgct
     9121 tttaacgggt gaggcgtgtt cttttaagca aatgctttat ttgtgcccgg aatcagcata
     9181 gttttccaat caaatggaaa aatttagggt gctgccgatt tgctttatgg ccgaggtgtc
     9241 aatggttagc caccggaata accattctgt gccaaaaaaa atcactacct ttgcagcctt
     9301 aaatagaata ttatggctac tcctagaagt gttgcattct atacattggg ttgtaagctc
     9361 aattattccg agtcctcatc gatttccagg atgtttgaaa acaatggtta ttcggtcaat
     9421 gaatttcatg aaggagcgga tatttttgtg atcaatactt gtagcgtgac ggattctgct
     9481 gataaaaaat gcaggaatat tgttcgccag gcgctgcgta tttctccaca ggcgaaggtg
     9541 gttgtaatcg gttgctatgc gcaattaaaa cctgaagaaa tcacccggat agaaggggtg
     9601 gacctggttt tgggtgctgc tgaaaaattc cggattattg aattcgtcga gagtataggc
     9661 aatgcccctt ctaaaggaat gatgatggcc ggtgatgtgc gcgaggccaa ccagtttgtc
     9721 tcttccttct ccatgggtga tcgtactcgt tcttttctga aggtgcaaga tggatgtgac
     9781 tataaatgca gcttttgtac cataccgatg gctcgtgggg caagcagaag cgatactacg
     9841 gctcaggtca tcgaaaatgc cagatatata gcatcccagg gcattaagga aattgtcttg
     9901 acgggggtga atcttggcga tttcggtaat ggaacagagg tgattgaagg tatttcgcat
     9961 aagaagaatg agctgttcat tgatctgatt aagaagctgg atgaagtgga aggtattgac
    10021 agattcagga tctcctccat agagccgaac cttcttacta atgaaatcat cgaattcgtg
    10081 gcacagagta agcgctttgt tcctcacttc cacataccac ttcagtcagg taataataag
    10141 atccttgctg caatgcgtcg caggtataaa tgtgaattat atgctgaccg tgtcagatat
    10201 attaaggaac tgatgcctca ctgctgcatt ggtgtggatg tcattgtcgg cttccccgga
    10261 gagactgatg aagatttttg ggagacggtg cgttttattc aaaacctgga tatcagctac
    10321 cttcatgttt tcacatattc tgaaagagcc aatacgccgg ccgcagagat gggacaggtg
    10381 cctgtacata tcaggcgaca aaggaatgat gtgcttggac aattgtctga ggttaagcgt
    10441 aaattgttct acgagaggca tcagggtgaa acaaggtcta tgctggttga gcatgggcac
    10501 tcaaatatgg aactaaccgg ttatacggat aattacatta aggtatcgct gccatatgac
    10561 gaaacattga tcaatcaggt tgtctctgta gaattggggg catttgatgt tgccggcaat
    10621 cttgtaggac atattgtatc cagtgaaatt atagtctgac catgtgtgga agagcatcac
    10681 taaccaaaaa tgaaagtgac ctggaggaga gctttggcgc aagcttctat acggaagaca
    10741 tagcgaggta tattccattg ccggccttca atataggtcc tgtacagaag ttgcctttca
    10801 ttgctcagga tgatccactg cattttcaga tcggttggtg gggatggaat gttacaatcg
    10861 gcaacaatac ccagttggta attaatgctc gcctggagga aatcgataaa aagaggacat
    10921 ttgttccaca tctcaatcat gggcgttgca tcatacccct tgacgggtat tatgaatggc
    10981 aaaaatgtgg aagtcagaaa attccctacc gaattacagt gccggatggc gggttgtttg
    11041 cagtggcagg actgtggcgg gaggaagttg ctaatgatgg tacggtctca aaacgcatcg
    11101 tcatcatcac tcgagcagct cagaaagata ttgccggtat t
//
LOCUS       DAOQMC010000057        11100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2285_length_11100_cov_5.232866, whole genome shotgun sequence.
ACCESSION   DAOQMC010000057 DAOQMC010000000
VERSION     DAOQMC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_11100_cov_5.232866"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKV31_03695"
     CDS             complement(<1..636)
                     /locus_tag="PKV31_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNB91990.1"
                     /translation="MKKNRIILNTGLGLIFLAGVLISVMSGKTPVKKIKPIYQTQQQW
                     VDSIMKTMTMEEKIGQLFMVRAHSDKGPDHIRFIKHLISTYKIGGLCFFQGTAEKQVD
                     LTNEYQKLASPVPLMISMDAEWGPAMRFKTGVVNFPKQMTMGAVQDNRIIYQFGREVA
                     RELLRIGVHINFAPDVDVNNNPANPVIGTRSFSENKYLVAQKAYMYMLGLQS"
     gene            complement(633..2627)
                     /locus_tag="PKV31_03700"
     CDS             complement(633..2627)
                     /locus_tag="PKV31_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91991.1"
                     /translation="MAKFNKSERVKTQKVITAPFPGSDADKLGNLIPTRLMPFLIVVF
                     AILFYANSLGGSYVMDDAIVISENQFTIQGVAGWKGIFSHDTFYGFFRESGKNHLVSG
                     GRYRPLSLALFALEGQVFGFKPFVFHLFNLLYYALTCFLLFRLFHYLFESRYGRTNAT
                     LIAFFCSLLFTVHPIHTEVVANIKGRDEILTCLLGLAGTWYFIRSIDRGRLALSILAA
                     FLWFLSLLAKENGIVFIALAPLAVWFFKDVKMSDILRYSIPAVVMTILYLILRRTVLG
                     PAPDSGPIMELMNNPFLTWHINGYLPVTFSIKLATIAVTMLKYILLLIYPYQLTSDYY
                     PQQIPLSDWSNWRAILGFLLYATAIVICLIRLKKKEIITFGILFYLVALFPMSNILFP
                     VGTLMSERFLFIPSIGFALIISRLIHIYVVKGSLNSRLNLLILALILILSVKTIARNK
                     DWSDNYTLFTTDVKISDNSAKMLNAAGGALLDSCIRVKDPIVKKQMAEEAQRYLLRAI
                     ELHPSYSNAYLLLGNSYAYTDNLEKAAENYQLALQYNQNFMDAKINLASMYKELGRKA
                     GEKEGNLPKAKEMLGKSLQLNPKDPETNRLLGIAIGMSGDPKGALIYFKAALEAKPDD
                     AFYMFDLGSAYANAGDMEKANYYHAEAIKKDPSLQQRLNK"
     gene            2797..3363
                     /locus_tag="PKV31_03705"
     CDS             2797..3363
                     /locus_tag="PKV31_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNB91992.1"
                     /translation="MTRFVVFCCLLSMPIMSFAQLTVSPKAGINYLWVGSRPQGFDRA
                     GAEQGWQAGLDLRLGKRFYFQPGVYYVSTGATISSLEDIKNIKTGNASFMKIPVNVGY
                     KLINSQLFKLRVHGGGTANKLLATRDGGALSASDFEPWNYGANVGAGIDLLFLALDIN
                     YEWGLTNMYSNNKNSLPTMASISVGLKF"
     gene            3469..6006
                     /locus_tag="PKV31_03710"
     CDS             3469..6006
                     /locus_tag="PKV31_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNB91993.1"
                     /translation="MMTRYFLLISFCLMTVTSQAQWKKKSKEEAKPKVTAPADTSAKK
                     SKDGLQPYDKVIGKDAVSRWGLFGVHNVKDKWYFEINDTILNRSMLAITRYVKTAAGG
                     GIYGGEQIKKLMLHWEKGPGDKIFMRGTTLIVNSPDSSKPIFQAVQNSNSEPIVGSFS
                     VKSIKKGDKGNSYVIDVTDFFNGDEHSFSLGPINKQRFKLKSLQKNSSFINKISSYPI
                     NVEIRTTKTYDIVPPSMDDRSAPSTGSYLPSGGDAGFVTMELNTSMILLPEKPMRKRS
                     FDSRVGFFSGQWTVYGEESQRSNLDTYVTRWRLEPKNASDAEKQLRGELIEPAKPIVY
                     YIDPATPDKWKPYLKQGVDDWNAAFETAGWKNAIRGEYWPENDTTMSLEDARFSVIRY
                     FASDIQNAYGPNVHDPRSGEILESHIGWYHNIMQLLRNWYLVQAGATDPKAGKREFDD
                     ELMGRLIRFVSSHEVGHTLGLRHNMGASYATPVEKLRDKDWTTKNGHTSSIMDYARFN
                     YVAQPEDGVTNLYPQIGDYDKWAIKWGYSNVGGKDDKEDAVILNNMIKEAYKNPRLRF
                     GTEISQYDPRYQTEDLGDNAMKASAYGIKNLQRILPKLIEWSVKDGESYTQLEEMYES
                     LVGQFRRYMGHVAKNVGGVYDDPTTSDMDVQPFRPVPKSIQKEAVAFLNQNLFTAPLW
                     LVDQNIISKIRPNAGVDAINKIQTSTLHNVLSGDKLIRLMEFSGTTGYSVEDLLNDLE
                     ANIIQKQSGVDIYQRNLQKAYVSKLISLLNPGSASSVELKIGTSYGSSPMTVNLNDTD
                     VPSIARGHLEKIRQTAKLRADTAANSSVRYHYKDILARIDKALKGDK"
     gene            6168..6776
                     /locus_tag="PKV31_03715"
     CDS             6168..6776
                     /locus_tag="PKV31_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNB91994.1"
                     /translation="MIDTIVFDLGGVLIDWNPMYVYKDYFPSKEDEQYFFEKVATFEW
                     NEEQDAGKPLIQATEERIRLFPEWEQPLRDFYGRWEEMLKGPIRDTVEIFRSLKETQA
                     YRFYALSNWSAENFHVALDRFEFLHWFDGRLISGEEKMAKPNPAFFKLLETRYDVIPQ
                     NTVYIDDNFRNYKAAEALGFLAIHFISPEQLKGELGRYSIEI"
     assembly_gap    7124..7223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7380..9656
                     /locus_tag="PKV31_03720"
     CDS             7380..9656
                     /locus_tag="PKV31_03720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNB91995.1"
                     /translation="MRLIPVLLLIFGISVYAQQKTAYDYVDPMIGTQRMGHVYPGATV
                     PFGMVQLSPDTDTIPYSVDGRYNPDVYKYCAGYQYGDKTIVGFSHTHFSGTGHSDLGD
                     FLIMPTQGPLQLNPGIASNPKSGYRSGFSHATEMARPGYYKVDLADHDITAEMTASER
                     VGVHRYTFHKAGLSHIILDLMHGIYNYDDKNIWTFVQVKDDYTVVGYRQTKGWARTRL
                     VYFAMQFSKPIKNYGCVNYDKREVYRGFWGKFDQEHNFPELAGKKLRVYFDFDMAQDE
                     ALEIQVALSAVGTEGAMANLGGETSGKNFDEIMQEAKASWDKELNKITMTGTEEDKVN
                     FYTALYHTLLSPIIYQDIDGKYRGLDQNIHQARGFKNYTIFSLWDTYRALHPLFNIIQ
                     PQRNRDMIRSMLAHYNQSAMQMLPVWSHYANENWCMIGYHSVSVIADAIIKDEYRGDA
                     NAALDACVKTARNRKYEGLAYYMDLGFIPEDKSSSSVSKVLEYAYDDWCIMKMAEKLG
                     RLDIQQEFGKRSGNFRNVFDPTTGFMRPKMSDGKFKKEFDPMDTHGQGFIEGNAWNYS
                     LYVPHDVPGLIQLLGNKGRFEQYLDQLFGKDLPDKYFEKTEDITREGIIGNYVHGNEP
                     SHHVAYLYNWTDSPWKTQQKVRMILKNQYRNGPDGLSGNDDCGQMSAWYLFSSIGFYP
                     VCPGSDQYMLGSPLVESATIRLENGNIFSVKTINQAPDNVYVKSITWKGFPWKERYIT
                     HEMIKNGGELVFEMSAKP"
     gene            9816..>11100
                     /locus_tag="PKV31_03725"
     CDS             9816..>11100
                     /locus_tag="PKV31_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB91996.1"
                     /translation="MYRPVFFLFFVIFWQTNIYSQETWTLERCIQEAIANNIQIQQTD
                     LAVRRDEIGVTTARAGRFPSLNASTSFYESFGRQIDPTTNSFNSQNFGNQSYNLNSGI
                     TLFNFNYINNTIRQNVENLKASKADKSQMERDIALNVAQAYLVILVAKENLEVSRKSL
                     EQTNEQLTMTDKLIAAGSKNRNARLELLAQQANNEAQIITAENDVTIGFLNLKQLMNI
                     APDLSMDIAPPAIGLPMENPDEIDYQALISYSIKSQPKFEALEYKLRSALLGEKIARA
                     QGLPSIQAQASAGTSYSTLSQRILSTTLERQQIPGVLLNDEPVSLSFLNPTVTTGKNP
                     YFNQLNQNLGFGLGVGLSIPIYNRNQVKASVQNARLNTENVRLQESQAKQQLLSDILR
                     ALTDAQAARRNLDAANKNYDALNGAYNDTQKRFEIG"
BASE COUNT         3197 a         2366 c         2572 g         2865 t
ORIGIN      
        1 ggattgaagg cccagcatgt acatataggc tttttgggcg acaaggtatt tgttttcgct
       61 gaaggatctg gtgcctatga cgggattggc cggattgttg ttgacatcta cgtccggtgc
      121 aaaattgata tgcacaccta tacggagcag ctcccttgcg acctctcgtc caaactggta
      181 gatgatgcga ttgtcctgga cagcacccat ggtcatttgc ttaggaaagt tgaccacacc
      241 ggttttgaag cgcatagcag gcccccattc ggcgtccatt gaaatcataa gcggaaccgg
      301 tgaagcaagt ttttggtatt cattggtcag gtccacttgc ttttcggcag ttccttgaaa
      361 gaagcaaagg ccaccgatct tgtaagtgct gatgagatgt ttgataaagc gtatatggtc
      421 gggaccttta tccgaatgcg ctctcaccat aaacagctgg ccaatctttt cttccatcgt
      481 cattgtcttc atgatggaat caacccattg ttgttgtgtc tgatatattg gtttgatttt
      541 tttcacagga gttttgcctg acatgacgct tatcaggacg cctgccagga agatcaggcc
      601 gaggcctgta tttagtataa tccgattttt tttcacttat tcaatctttg ctgcaaggac
      661 ggatcttttt tgattgcctc tgcatgatag taatttgcct tttccatgtc tccggcattg
      721 gcgtatgcac taccgaggtc aaacatgtaa aaggcgtcat caggttttgc ttcaagagca
      781 gccttaaaat aaattaaagc accctttgga tcgcctgaca ttccgatcgc tattcccaac
      841 aatctgttgg tctcaggatc tttaggatta agttgcagcg atttgccgag catttcttta
      901 gcttttggca gattgccctc cttctcacct gcctttctgc ccaattcctt gtacatactt
      961 gccagattga tttttgcatc cataaaattc tgattgtatt gcaatgcaag ctgataattt
     1021 tccgctgctt tttcaagatt gtctgtgtag gcgtaggaat tacccagcag cagataggca
     1081 ttgctgtatg aaggatggag ctcaatcgcg cgtagaagat agcgctgagc ctcctctgcc
     1141 atctgtttct tcacaatcgg gtccttcact ctgatacagc tatccagtaa tgcgccaccc
     1201 gcagcattga gcattttagc actgttgtcg gatatcttga catcggtagt aaacagcgta
     1261 taattgtcac tccaatcttt attgcgagct attgttttaa cgctcaaaat taggattaaa
     1321 gccagaatta aaagattgag acgggaattt aaacttccct taacaacata gatatgtatc
     1381 aatcggctga ttatcaatgc aaaaccaatg gatggaatga aaaggaaacg ttctgacatg
     1441 agtgttccca ccggaaagag aatatttgac attggaaaca aggcaaccag atagaaaagt
     1501 atgccaaatg tgatgatttc cttctttttc aatctgatca gacagatgac aatggcagtg
     1561 gcatacaata gaaaacccag aattgcacgc cagttggacc aatcggacaa tggaatttgt
     1621 tgaggataat aatcactggt caattgatat gggtagatca gcagtaagat atatttgagc
     1681 atagttactg ctatggtggc gagttttatt gaaaatgtaa ccggcaggta gccattgata
     1741 tgccaggtaa ggaaggggtt attcatcaat tccatgatgg gaccgctatc cggagccggg
     1801 cccaaaactg ttcttcgaag gatgaggtaa aggatggtca tgacaactgc gggaatgctg
     1861 tatcttagga tgtcagacat ttttacatct ttaaaaaacc acacggcaag tggagctaac
     1921 gcgatgaaaa cgatgccgtt ttccttagcc agcaaggaga gaaaccataa aaaagccgcg
     1981 agaatggaaa gagctaaccg tcccctgtca atgcttcgaa taaaatacca cgtccctgca
     2041 agacccagca gacaggtcag tatctcatcc cgacctttga tattggcaac tacttctgtg
     2101 tgtataggat ggacggtaaa aagtagggag cagaagaagg caatcagcgt ggcattggtc
     2161 cggccatatc tgctttcaaa aagataatgg aatagcctga acaataaaaa gcaggtcaga
     2221 gcataatata acaggttgaa aagatgaaag acaaagggct tgaagccgaa tacctgtccc
     2281 tccagggcaa acaaggcaag ggaaagaggc cggtatcttc cccctgacac caaatggttt
     2341 tttccacttt ccctgaagaa gccgtaaaag gtatcgtggc tgaagatgcc tttccatcct
     2401 gcaacaccct ggatagtaaa ttgattttca gaaataacga tggcatcgtc catgacatag
     2461 gatccgccta aactgttggc ataaaatagt atggcaaaaa caacaatcaa aaaaggcatc
     2521 agtctggtgg gaatcaaatt gcccaatttg tctgcatctg atccgggaaa gggagcagtt
     2581 ataacctttt gggttttgac tcgttcggat ttgttaaatt ttgccatgat tgcccgttga
     2641 aaattaaggt ttctcttaat taaattttaa aaaattaaaa aatacagcag ttacaaaaat
     2701 aattatttca cttgaatcgc ttaaaaaatg tgcgactgga taaccacaat atcaggtgca
     2761 tccgacgatg atttattaac ctaaaaaatg cagatcatga cacgattcgt agtattttgt
     2821 tgccttttga gcatgccgat tatgagcttc gcccaattaa cagtgagtcc taaagccgga
     2881 atcaattatc tctgggtcgg aagcagacca cagggttttg accgggccgg tgcagaacag
     2941 ggctggcagg caggtctgga tctgcgactc ggcaagcggt tttatttcca acccggtgtc
     3001 tactatgtaa gtaccggagc tactatttcc tctctggagg atatcaaaaa catcaagacc
     3061 gggaatgcct ctttcatgaa aatacccgtc aatgtaggat ataaactgat taattctcaa
     3121 ttgttcaagc tcagggttca cggtggtggg acagcaaaca aactcctggc aaccagggat
     3181 gggggtgctc tttctgcgtc agattttgag ccctggaatt acggtgcaaa cgtaggtgcc
     3241 ggcatcgacc tgttgttttt ggctctcgat atcaactatg aatggggcct gaccaatatg
     3301 tattcaaaca ataaaaacag cctgccgacg atggcaagta tttctgttgg attaaagttc
     3361 tgacaagact cgagcagaag gtgtttcaaa tagccgtggt agtgtaatgc ctcggctatt
     3421 ttttatttca ggtaaaaatc cttagtttta cccgaaagag taaattcaat gatgacacgt
     3481 tattttttat tgattagttt ttgcttgatg actgtgacgt cgcaagcgca atggaagaaa
     3541 aaatccaaag aggaggctaa gcccaaggtg actgcacctg ctgatacttc cgcgaagaaa
     3601 tctaaggacg gattgcagcc ttatgacaag gtaataggaa aggatgccgt gagtcgctgg
     3661 ggcttgtttg gggtgcataa tgtcaaggac aaatggtatt ttgaaatcaa tgataccata
     3721 ctcaaccggt ccatgttagc cattaccagg tatgtcaaga cagctgccgg tggaggtata
     3781 tacggaggag agcagatcaa gaagcttatg ttgcattggg aaaagggtcc cggagataag
     3841 atctttatgc ggggtacgac cctgatagtc aacagtcctg acagctcaaa gcctatattc
     3901 caggcagtcc agaattctaa cagtgaaccg attgtaggtt cgttttcagt caaatccatc
     3961 aaaaagggag ataagggcaa ttcgtatgtc attgacgtga ctgacttttt caatggggac
     4021 gaacatagtt ttagtcttgg accaatcaat aagcaacggt ttaagctaaa aagcctgcaa
     4081 aaaaattcct ccttcatcaa caaaatttct tcatatccca tcaatgttga gatcagaacg
     4141 accaaaactt atgatatcgt gccgccctct atggacgatc gctcagcgcc ttccactggc
     4201 agctatttac cttcaggagg agatgctggt tttgttacca tggagttgaa cacatccatg
     4261 atacttttac cggagaagcc catgaggaag agaagttttg atagtcgggt aggcttcttt
     4321 tccggccaat ggacagtcta tggagaagaa tcccagcgat caaacttaga tacctatgtt
     4381 acacgctggc gtcttgagcc caaaaatgcc tctgatgctg aaaaacaact acgcggagaa
     4441 ctgattgagc ctgctaagcc catcgtttac tatatagatc cggctacacc tgataagtgg
     4501 aaaccttatc tgaagcaggg cgtcgatgat tggaatgcgg catttgagac agcaggctgg
     4561 aagaatgcca tccgcggaga gtattggccg gaaaatgata cgacaatgag ccttgaagat
     4621 gcacgttttt ccgtcatcag atatttcgca tccgatatcc agaatgctta tggacctaat
     4681 gttcacgacc cacgcagcgg agagatactg gaaagccaca tcggctggta tcacaatatc
     4741 atgcagctgt tgcgcaactg gtacctggtt caggccgggg ccaccgatcc caaagcaggc
     4801 aagcgtgagt ttgatgacga gttgatgggt cggcttatcc gatttgtttc atcacacgaa
     4861 gttggtcaca cgctcggtct gcgacataat atgggggcaa gttatgccac acccgttgag
     4921 aaattgcgag acaaagactg gacaacaaaa aatgggcata cttcctcgat catggactat
     4981 gctcggttca attatgtcgc tcaaccggaa gacggtgtca ccaacttata tccacagatc
     5041 ggagactatg acaagtgggc gatcaaatgg ggttacagta atgtaggggg caaggatgac
     5101 aaggaagacg ctgtcatcct caacaacatg attaaggaag catacaaaaa cccgagacta
     5161 cgattcggta cagaaataag ccagtacgat ccacgctatc agacagaaga cctgggggat
     5221 aacgccatga aagcctctgc ctacggcata aagaacttac agcgcatcct gcccaaattg
     5281 atcgagtgga gtgtaaagga tggcgaaagt tatacacaac ttgaagaaat gtacgaatca
     5341 ctggtaggcc agttcaggag atatatggga catgtggcaa aaaatgtcgg cggtgtgtat
     5401 gatgatccga cgacaagtga tatggacgtc cagccattca ggccggtacc aaagtcaatc
     5461 cagaaggaag cggttgcatt cctcaatcaa aacctgttta cagcaccatt atggctggtc
     5521 gatcagaaca taatttccaa gattagacca aatgcaggtg ttgatgccat caataaaatc
     5581 cagacatcga cactccacaa cgtgctttcc ggtgacaagc tcatccggtt gatggaattc
     5641 tcagggacaa ccggttattc ggtagaagat ttactcaatg atcttgaggc gaatatcatc
     5701 cagaaacaaa gtggagtcga catctatcaa cgtaacttac aaaaggcata tgtatcaaaa
     5761 ctgatctccc tgctcaaccc gggatcggcc tcatccgtag agttgaagat aggaaccagt
     5821 tacggctcga gccctatgac tgtcaatctg aatgataccg atgtgccttc catcgcacgc
     5881 gggcatttag agaaaatcag gcaaacggct aaacttaggg ctgacacagc agcgaattct
     5941 tcggtcaggt atcattataa ggatattctg gcgcgaatag ataaggcttt gaaaggcgat
     6001 aaataattaa tgaaaggccg gtcgggagat ccggcttttt taccacttgt atttccgtag
     6061 gcgttggcat caaagggaca acctttgtct tcggctgccg ttgtatacgt agtttcgaat
     6121 caccgaaaac aacatgattg atgtcctcgt atattaataa ttcaaacatg atagatacga
     6181 tagtctttga ccttggcggt gtcctgatag actggaatcc catgtatgtc tataaagatt
     6241 attttccttc aaaggaggat gagcaatatt tttttgaaaa ggtcgccacc tttgagtgga
     6301 atgaagaaca ggatgcgggt aagccactga tacaagctac tgaagagcgt atccgactgt
     6361 ttccggaatg ggagcagcct ttgcgtgatt tttatggccg atgggaggag atgcttaagg
     6421 ggcctatccg ggatactgtg gagatattca ggtcgctgaa ggaaacgcaa gcctatcggt
     6481 tctatgcatt gagcaattgg agtgcagaaa atttccatgt cgctcttgac aggtttgaat
     6541 ttttgcattg gtttgacggc aggctaatca gtggggaaga aaagatggca aagccaaatc
     6601 cggctttttt caaactactc gaaacaaggt atgatgtcat ccctcagaat acggtctata
     6661 tcgatgacaa tttcaggaac tataaggctg cggaggcctt aggtttcctg gcgattcatt
     6721 tcatttcgcc ggagcagtta aagggggagt tagggagata ttcgattgaa atatgagtat
     6781 ttcggatgcc cgacctgaag taggtagatg attaatagcc gagtgttccc tgttggtgtc
     6841 catgtggtga taatgtgatt gtcctgatgt tacaatattg cggaaattga tttgctaata
     6901 gtctgaaaag acaagcagca caaaggttct gacttattat tttcctaccc acttcggcac
     6961 gatctgcgtt ttctcccgac ttcagtaccg catccacctt agcctttatc cgggcctctt
     7021 aagacaacca ttctcaattg gggagttaga aaactattcg attgattttg agtatttcgg
     7081 atgcccgacc tgaagtaggt agatgattaa tagccgagtg ttcnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnngacttat tattttccta cccacttcgg cacgatctgc
     7261 gttttctccc gacttcagcg ccgcatccac cttagccttt atccggacct cttaagacaa
     7321 ccattctcaa ttcttgccaa ctctccttat attcgccaga caaatatgtt tttgccggta
     7381 tgcgtttgat tcctgttctg cttcttatct ttggtatttc tgtatatgct caacaaaaaa
     7441 cagcctatga ctatgtcgat cctatgatcg gcacacagcg catgggccat gtatatccag
     7501 gcgcaacggt gccctttggc atggttcagc taagtccaga taccgacacg attccctatt
     7561 cggttgatgg tcgatacaat cctgatgtat ataaatactg tgccggttat caatatggcg
     7621 ataaaaccat cgtaggcttt agtcatacgc acttctccgg taccggtcac tccgacctgg
     7681 gagattttct gatcatgccg acccagggac ccctgcaact caatccaggc attgcatcca
     7741 atcctaaaag cggctatcgg tccggctttt cccatgcaac cgaaatggcc aggccgggat
     7801 attacaaggt tgacctcgca gatcatgaca taaccgcgga gatgacggca agtgaaaggg
     7861 tcggagtgca cagatacaca ttccataaag ccggattgtc ccatatcatt ctcgacctga
     7921 tgcacggcat ttacaattat gatgacaaaa atatctggac ctttgttcag gtaaaggatg
     7981 actatacggt cgttggatat cgtcagacca agggctgggc gcgtacaagg ctggtgtatt
     8041 ttgccatgca attttccaag ccgatcaaaa actacggctg tgtcaattat gataaaagag
     8101 aggtctatcg gggcttttgg ggcaaattcg accaggaaca caacttcccg gagctggcgg
     8161 gcaaaaaact gagggtatat ttcgactttg atatggcgca ggatgaggcc ctggaaattc
     8221 aggttgccct gtctgccgta ggaaccgaag gcgccatggc taatctggga ggtgaaactt
     8281 ccggaaagaa ctttgatgaa atcatgcagg aagccaaagc atcctgggat aaggagctca
     8341 acaagataac catgacagga acggaggagg acaaggtcaa tttctataca gccctttacc
     8401 ataccttgct ttcacccatc atctatcagg atattgacgg aaaataccgg ggcctcgatc
     8461 agaatattca tcaggcaaga ggctttaaga actataccat attctccctg tgggacacct
     8521 atagggcatt gcatccactg ttcaatatca tccagccaca acgcaaccgg gatatgatcc
     8581 ggtcgatgct tgcccactat aaccaaagtg ccatgcagat gttgcccgtg tggagccatt
     8641 atgccaatga aaactggtgc atgataggct accacagcgt atccgtcatc gcagatgcca
     8701 tcatcaagga tgaatatcgg ggagatgcca atgcagcgct ggatgcctgt gttaagaccg
     8761 caagaaacag aaaatatgaa ggcctggctt attatatgga tctgggcttt attcccgaag
     8821 ataagtcgtc atcatctgtg tcaaaagtac tggagtatgc ctatgacgac tggtgcatca
     8881 tgaagatggc agaaaagctc ggcaggttgg atatccaaca ggaattcggc aagaggtccg
     8941 gtaatttcag aaatgtattt gatccaacaa caggcttcat gagaccaaag atgtcagacg
     9001 gtaagttcaa gaaagagttt gacccgatgg acactcatgg tcagggcttc atagaaggga
     9061 atgcctggaa ttatagcctt tatgtacctc acgatgtgcc gggactaatc caattgttag
     9121 gtaataaggg acgttttgag caatacctcg atcaactttt tggaaaggac ctgcctgaca
     9181 agtatttcga gaagaccgaa gacataaccc gtgaaggcat catcggcaac tatgtacacg
     9241 gaaatgaacc gtcccaccac gtagcctacc tttacaactg gactgatagc ccatggaaga
     9301 cccagcagaa agtaaggatg atccttaaaa atcaatacag aaacggaccc gacggcttga
     9361 gtggcaatga tgactgtggc cagatgagtg cctggtattt attctcttct atagggtttt
     9421 atccggtgtg ccccggttcg gatcagtata tgctgggcag tccattggtg gaaagcgcca
     9481 caattcggtt agaaaacgga aatattttca gcgtaaaaac catcaaccag gccccggaca
     9541 atgtatatgt caaaagtatc acctggaagg gcttcccctg gaaagagaga tacattaccc
     9601 acgaaatgat aaagaatgga ggggagctgg tatttgagat gagcgccaag ccatgatggt
     9661 tactttagga aaagtattgg gacaaaaata gccaaacggg gctaaattta tttcgtaata
     9721 taaaactggc atttcacgac atattaaatt gatttggtag attttttttt gataaacagc
     9781 tcttgtaact tatcattgtt gaaaaatttt aggttatgta caggcctgta ttcttcctgt
     9841 tcttcgtaat attttggcaa acaaatattt atagtcagga gacatggaca ttggagcgtt
     9901 gcatccagga ggcaatcgct aataacatcc agattcagca aaccgacctt gctgtcagac
     9961 gtgatgagat aggtgtcacc actgcccggg caggccggtt ccccagcctg aatgcaagta
    10021 cgtcctttta tgagagtttt ggccgacaga ttgaccccac caccaatagt ttcaatagtc
    10081 agaattttgg caaccaatcc tataatctga attccggtat aacgttgttt aatttcaatt
    10141 atatcaacaa taccatccgc cagaatgtgg aaaatctaaa ggcttctaaa gcggacaaga
    10201 gtcagatgga gcgtgatata gcccttaatg tagcacaggc ttaccttgtt attttggtag
    10261 cgaaggagaa tcttgaagtg tcacgaaaga gcctcgaaca gaccaatgaa cagttgacga
    10321 tgacggacaa gcttatagct gcgggatcaa agaatcgcaa tgcccgactg gaattacttg
    10381 cccaacaggc aaataatgag gcgcagatta tcactgccga aaatgatgtg accattggtt
    10441 ttctcaattt gaaacaactg atgaatattg ctcccgatct cagtatggat attgctcctc
    10501 cagctatcgg cctcccgatg gaaaacccgg acgaaatcga ctatcaagca ttaatctcat
    10561 attcaatcaa gtcacaacca aaattcgaag ccctggaata taagcttcgc agtgccttgt
    10621 tgggagaaaa aatcgccagg gcacaagggc ttccttccat tcaggcccaa gcatctgcag
    10681 ggacaagcta ttcgacatta agccaacgca tcctgagcac tacccttgaa agacaacaga
    10741 tacctggtgt cctgctcaat gatgagccgg tcagcctgtc atttttgaat ccgacggtga
    10801 caacagggaa gaatccctat ttcaatcagt tgaatcaaaa ccttggtttt ggtcttggtg
    10861 tcggcctaag cataccgatc tacaaccgca atcaggtaaa agcttctgtg caaaacgcca
    10921 gactaaatac cgaaaacgtc aggctccagg agagtcaggc taaacaacag ctgctttcgg
    10981 atatattaag agcattgaca gatgctcagg ccgccagaag gaatctggac gctgccaata
    11041 aaaactatga tgcgctgaat ggcgcttata acgacacaca aaagcgattt gagattggcg
//
LOCUS       DAOQMC010000058        10718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2376_length_10718_cov_4.644284, whole genome shotgun sequence.
ACCESSION   DAOQMC010000058 DAOQMC010000000
VERSION     DAOQMC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_10718_cov_4.644284"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKV31_03730"
     CDS             complement(<1..372)
                     /locus_tag="PKV31_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91997.1"
                     /translation="MRLFFKSIFLNNRFFYAGGVIVVIMFLGFFWPLLIGPAFTLMGL
                     LGLATLMDVFLLWRKSGITASRIMSEKFSNGDENIVKIPLVNKYGMDIGIRIIDELPE
                     QMQERNFKLTGKLKSGTSKTMV"
     gene            complement(377..1360)
                     /locus_tag="PKV31_03735"
     CDS             complement(377..1360)
                     /locus_tag="PKV31_03735"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB91998.1"
                     /translation="MEENLMEFHSRIDLAGVTDKLNRIKVEIGKVIVGQERNIDLILV
                     ALLANGHVLLEGVPGVAKTLLAKLFARTISAGFSRVQFTPDLMPSDILGTSVFDLKTS
                     AFTFKKGPVFSNIILIDEINRAPAKTQAALFEVMEERQITIDGQQFRMEPPFLVIATQ
                     NPVEHEGTYRLPEAQLDRFLFKIEMLYPELNEEVEILRKDHHNRAEQKQMVVDPVVTG
                     EEIVGMQQVIQGVIVEDNLLEYIATITKATRENQSLYLGGSPRASIALLKASKANAAL
                     HGRDFIMPEDIKMVAAPVLQHRVVVAPAQEMEGVTPAMIIKRIVDSIDVPR"
     gene            complement(1367..2551)
                     /locus_tag="PKV31_03740"
     CDS             complement(1367..2551)
                     /locus_tag="PKV31_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB91999.1"
                     /translation="MIRRSYIIIFMAAMIVAFLVARNTSRKPSKLDWNPYYNISTKKP
                     LGLYVLNKEASNLFSEKTITRVNGTPGSYFNKLIEKKEYAANTFVFIGDEMPWEEEEI
                     ENLLTAVDAGMNGFISLNEFPGSMLEKLELRMQEDNESAFVRLSIEEGDSVNIEPAQS
                     GQFNSYFMSYFLPIGRRSRNVLGRNGRFPNFIKVFYGEGTILLHCSPAVFSNYYLLKS
                     DIKPYTESVLSMLPGKKIVWFTGVQQSNISGSPLSFILANPPLRYALYMIVAGVMLFI
                     LFNAKRRQRVIPVRTPNRNTSVEFVRTIGNLYYQKISIPDLLKMRLGFLKERLKTDYQ
                     MSLDQNDAELIDRLSNKTGKPVDLITGFVDLIRDVEAGPPDSKQMLTQFEQYIYRIFI
                     LK"
     gene            complement(2548..3324)
                     /locus_tag="PKV31_03745"
     CDS             complement(2548..3324)
                     /locus_tag="PKV31_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNB92000.1"
                     /translation="MTRHLLILLLFFHCFSLSWAAGTNDGITEEYQEDYTEKKDYSGL
                     FQPDNSDTRAIEKARFDENLPEKYKSIIKSYETAPPKEVRKSWFDNLLRKIFGNLPSI
                     NFLGISKLFQILIYAGIGFLAIYLIRLIISYGFDWPFGTKKVEGPEQLEDTMEVTGSE
                     DLSTAIRKAVSEGNFRLAVRYWYIHLLRFSDRNAIISYHPEKTNSDYVHEITNPDFKA
                     KFTELSRIYDFVWYGELTIGERDYWHIAERFDHLVNMLSA"
     gene            complement(3321..4286)
                     /locus_tag="PKV31_03750"
     CDS             complement(3321..4286)
                     /locus_tag="PKV31_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNB92001.1"
                     /translation="MKETAFIQQNKEKWLAFEAFASGSLSVDAGELSDLYVSLVNDLA
                     YAQTYYPKSITVAYLNQLCAAAFQKIYRTRRRDSNRLVDFYKVEVPMLMVEYRRYLLL
                     ASIVFGLSVGIGVLSSIYNESFVRLILGDDYVNMTLDNIANGNPVAVYKSGSNWGSFL
                     GITFNNLGVSLKAFIYGITAGLGTVYIALSNGVMLGTFQYMFVREGVFWDSVRGIWIH
                     GSMEIFSIVISIMAGLILGAGILFPGTYSRVQSFRKSFEAGLKIFFSTVPFFIAAGFL
                     EGFMTRYAIEMPAFASFGIILTTLALISYYYLVYPYRLMKKITPI"
     gene            4310..5041
                     /locus_tag="PKV31_03755"
     CDS             4310..5041
                     /locus_tag="PKV31_03755"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNB92002.1"
                     /translation="MEYITINTTQNVNINFPLAGTGSRIAAFLIDLVIKYVYIIIVIQ
                     LLSHSDLEFSIDQWSASAIYIICLSPIFFYSLVWEYFNNGRTPGKMVVKIRVAKIDGY
                     QADFIDIFTRWAMRMIDIQLGFGLIGLITMTRNNKNQRLGDIAAGTSVISQAKTFKFE
                     QTIFEELHKNYTPTYPDVIKLSDNDIRIIKENLEKSRKFNDLSLILRLKYKVEDILGI
                     KSSEDPYIFINKVITDYNYYTQESD"
     gene            complement(5067..7508)
                     /locus_tag="PKV31_03760"
     CDS             complement(5067..7508)
                     /locus_tag="PKV31_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNB92003.1"
                     /translation="MRVFLSIWACLYFISLSAQPATISPFIKVDQFGYLCNSKKIAVI
                     SDPINGFNASESFSPGISTGAYQVRKWNGGDVIMSGTISPWNGGAVHTQSGDRGWYFD
                     FSSVTDPGIYYIWDNVNQVGSGQFFIGSNVYDANLRAVLRTYYYARCNYAKSMPYTEK
                     PEYNDVAAYEGSNQDKHARSSLDKNNPSTERDVSGGWFDAGDMNKYVTFAALPMLTLM
                     DSYYYFPQVFGDDNNIPESGNGIADILDEVKWELLWLVKMQEGTGTNGLFLKVGVDSY
                     NCSNSPPSADVCTRYYIPECTSSTIAGAAIFAAAHRIYSGVTGQGAFAADMLARAQSA
                     WARAIVTTSGWTNFQTSCDNGDIKSGDADWDSETQRLYALTAAIYLYAATGNNNYKSY
                     VENNYSTLNPIANGWWGPYNVAIGRAMLYYTTLPGASTTVKNNILSSLAGTYTGMGLN
                     EYISNTDLYRSHMPNAQYHWGSNEVKAYIGAQQSDFVNYNLNPGNHDQYKELAEQYLH
                     YFHGINPLGIVYLSNMYPLHAEACVNEIYHGWFGDGTPYDNALTSQYAAPPGFIVGGA
                     NASFTIPSISPPSGQPPQKSYKDWNTGWNTSTNMNENSWECSEVSIYVQAAYINLLAS
                     VLGLSVNPGCDPVILATHALEFAADRRGVEVLLKWQGNRDVVRYRVQMSNDGSLFNTV
                     FSTEGREGTFQWTDRPVIESAVVYYRLEITEINGRVSYSPVRAITWPDFSRIEVVTDN
                     GAGNVTLRMAENKAHQKFDIRICSTTGICLFHKKDNLATELVVDTAHWPSGMYFVYVT
                     GDDYPTEVFKVYVLK"
     gene            7701..8540
                     /locus_tag="PKV31_03765"
     CDS             7701..8540
                     /locus_tag="PKV31_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB92004.1"
                     /translation="MTIIIVKSFLVIVLAWKLWEASKEGYQLSIDREFFTFLMIGFLA
                     ELVDGSLGMAYGVISSSFLIFFGVSPVVASAGVHTSEVFTTGVSGLSHLHFKNVDKGL
                     FFRIVIPGVIGSLIGAIVLSKLVDGEIIRPFIAVYLLLVGIKLIAREMQGDKVHIKPL
                     KSVPIIGFFGGALDAIGGGGWGPLVTSTILSRSASNAKHTIGTVNTAEFFVTFASTGI
                     FMFLIGISFFNILLGLIIGGVVAAPLGAYFLKHVPHKVLMYVVGSVLIIVSAFSIYYY
                     IFK"
     gene            complement(8997..9251)
                     /gene="rpmA"
                     /locus_tag="PKV31_03770"
     CDS             complement(8997..9251)
                     /gene="rpmA"
                     /locus_tag="PKV31_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNB92005.1"
                     /translation="MAHKKGVGSSDNGRDSKSKRLGVKKFGGQAVIAGNILVRQRGTR
                     FHPGVNVGCGKDHTLFALTSGVVAFKKGKKDRTFVDVVMN"
     gene            complement(9283..9594)
                     /gene="rplU"
                     /locus_tag="PKV31_03775"
     CDS             complement(9283..9594)
                     /gene="rplU"
                     /locus_tag="PKV31_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNB92006.1"
                     /translation="MFAIVSIAGQQFKVEGGQKIFVHRLDAKEGDKLTFDQVLLTSDG
                     NNVAVGTPTVSGVAVTATVLSEMVKGDKVIVFKKKRRKGYRVKNGHRQQFTHLQIDSI
                     G"
     gene            9907..10161
                     /locus_tag="PKV31_03780"
     CDS             9907..10161
                     /locus_tag="PKV31_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNB92007.1"
                     /translation="MKKDIHPSSYRKVVFKDISTDEAFITQSCVNTKDTIVWEDGNEY
                     PLVKVEISSFSHPFFTGKMKFVDTAGRIDKFNKKFAKFMK"
     gene            10257..>10718
                     /locus_tag="PKV31_03785"
     CDS             10257..>10718
                     /locus_tag="PKV31_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNB92008.1"
                     /translation="MKIVLYDGPEWKDLLPLTFTKPVAGLRIGIDTIREKWEAALNTE
                     CLIKTQDYLQAIHPVATSDDFLFINASYIPHPVLTNHILSIRHNQSLAISGQVIAYRT
                     DSFHVASSEAKQFDIISINAPEDVIHICYPWDIFSNNNRVLQQDFDRITSGR"
BASE COUNT         3236 a         2368 c         1976 g         3138 t
ORIGIN      
        1 caccattgtt ttggatgtcc cggatttcaa ttttcctgtc aacttgaaat tcctttcctg
       61 catctgttcg ggcagttcat cgattatcct gataccgata tccatgccgt atttgttgac
      121 caatggaatc ttcactatat tctcatcacc attcgaaaat ttctcagaca tgatacggga
      181 agctgttata ccggatttgc gccacaaaag gaaaacatcc attagtgtag ccaaacccag
      241 cagccccatt aaagtgaagg ccggacctat caacaatggc caaaaaaagc ccaggaacat
      301 gattactaca atcacacctc cggcataaaa aaaccgatta ttcaggaata tgctcttgaa
      361 aaacaatctc attcagttac cttgggacat cgatagaatc tacaattctt ttgataatca
      421 tagccggggt cacaccttcc atctcctgcg caggggcgac gactacacgg tgctgcaata
      481 caggcgctgc caccatcttg atatcctcag gcatgatgaa atcccgtcca tgaagtgcgg
      541 cattggcttt agatgctttc aagagggcaa tagatgctct cggtgaacca cctagataga
      601 gcgattgatt ctcgcgggta gctttggtta ttgtggcaat atattcaagc aggttatcct
      661 ccacaattac accttgaatg acttgctgca taccgacaat ctcttcgccg gtgacaacag
      721 gatccactac catttgtttt tgttctgctc tgttgtgatg atcctttcta agaatttcca
      781 cctcttcatt caactcggga taaagcattt ctatcttgaa aaggaagcgg tcaagctgcg
      841 cctccggcag acggtaagtc ccttcatgct ctactggatt ttgcgtcgca atgactaaaa
      901 aaggtggctc catcctaaat tgttgtccat cgattgttat ttggcgctct tccattactt
      961 caaacaaggc agcttgtgtc tttgcaggag cacgattgat ttcatcaatc aggataatgt
     1021 ttgagaacac tgggcctttt ttgaaagtaa aagccgaagt tttaaggtca aataccgatg
     1081 ttccgagaat atcactaggc atcagatccg gagtaaattg cacccttgaa aagccggcac
     1141 taatcgttct ggcaaataat ttagccagta aggttttggc aacacccgga acaccctcaa
     1201 gtaagacatg accattggcc agtaaagcaa ctaatatcag gtctatattt ctttcctgac
     1261 caacaattac ctttcctatt tcaaccttta tccggtttaa tttgtcagta actccggcca
     1321 gatctatgcg tgaatgaaat tccattaggt tttcttccat tgtgatttat tttaatatga
     1381 atatcctgta aatatattgt tcaaactgag tcagcatttg ctttgaatcc ggaggaccgg
     1441 cctctacatc tcgtatcaag tccacaaaac ctgtaatcag gtcaacaggc tttccggttt
     1501 tgttggaaag tcgatcgatc aactcggcat cattttggtc taatgacatt tggtaatccg
     1561 tcttcagtct ttctttcaga aatccaagcc tcattttcaa taagtctggt attgaaattt
     1621 tttgataata aagattgcca atggtccgga caaattcaac cgatgtatta cgattcggtg
     1681 tcctgacagg aatgaccctt tgccttctct tggcattgaa caagataaag agcatcactc
     1741 cggcaacaat catatacaat gcatacctta atgggggatt agcaaggata aatgaaagcg
     1801 gcgaacctga aatattggac tgctgtacgc ccgtaaacca cactattttt tttcccggta
     1861 gcatacttaa tacactctct gtgtatggtt ttatatctga ctttaacaga taataattgg
     1921 aaaaaacagc aggagaacaa tgcaacaata tagtcccttc accataaaaa accttaataa
     1981 aattgggaaa tctaccattg cgcccaagta cattccttga tctcctgcca atgggcaaaa
     2041 agtaactcat aaaataagag ttgaactggc ctgactgtgc aggttcaata ttgacactat
     2101 ccccctcttc tatgctcaac ctgacaaatg ccgattcatt gtcttcctgc atacgcaatt
     2161 ccagcttttc aagcatacta cccggaaatt cattcaggct aatgaagcca ttcataccag
     2221 catccacagc agtcaacaaa ttctctatct cctcttcctc ccagggcatt tcatcaccta
     2281 tgaatacaaa agtattggca gcatactcct ttttttcaat gagcttgttg aaataactcc
     2341 cgggagtacc atttaccctg gtaatggttt tttccgaaaa caaattactt gcttccttat
     2401 tgagtacata taatcccaga ggcttcttag tactgatatt ataatatgga ttccaatcca
     2461 acttagaagg cttccttgat gtattcctgg ccacaagaaa agcaactatc atagctgcca
     2521 tgaagatgat gatataagaa cgcctgatca tgcagaaagc atatttacca ggtgatcaaa
     2581 acgttctgca atatgccagt agtcgcgctc tccaatcgta agctctccat accacacaaa
     2641 atcataaatt ctggataact ctgtaaattt tgccttaaag tctggatttg tgatttcatg
     2701 aacataatcc gaattagtct tctccggatg atatgaaata atggcgtttc ggtcggaaaa
     2761 cctaagcaaa tgaatatacc agtaccgtac cgccaaccta aagttacctt cactaactgc
     2821 tttacggatt gctgttgaaa gatcctcact ccctgtaact tccatagtat cttccagctg
     2881 ttcaggacct tcaaccttct ttgtcccaaa tggccaatca aatccgtacg aaataatcaa
     2941 ccggatcaaa taaatagcaa gaaagccaat ccccgcatat attagtatct gaaacaactt
     3001 tgaaataccc aagaaattaa tagaaggcaa attaccaaat atcttcctta acaaattatc
     3061 aaaccaggat ttcctgacct cttttggagg tgccgtctca taagacttga taatgctttt
     3121 atatttttca ggtaaattct catcgaaacg agccttttct atagcccggg tgtcagaatt
     3181 atccggctga aacaatccac tgtaatcctt tttttctgta taatcttcct gatattcctc
     3241 agttattcca tcgttggtac cagcagccca agaaagactg aaacaatgga agaataatag
     3301 taaaatcaag aggtgtcgtg tcatattggg gtgatttttt tcatcaatct atatggatag
     3361 acgagataat aatatgaaat caaggctaag gtagtcagga taataccaaa acttgcaaaa
     3421 gccggcattt caatagcata ccttgtcatg aagccttcta aaaatcccgc agcaataaaa
     3481 aatggcactg tgctgaaaaa aattttcaac ccggcttcaa atgactttcg gaaagattgt
     3541 accctggaat aggtccccgg aaacaatatt cctgcaccga gtataagacc ggccataatg
     3601 cttattacga ttgagaatat ctccattgat ccgtgaatcc aaatacctct gacactatcc
     3661 caaaacacgc cttcccgaac aaacatatac tggaaggttc ccagcataac tccgttggac
     3721 agcgcaatat agacggttcc cagtcctgca gtgatgccat aaataaaggc cttgagactg
     3781 actcccagat tattaaaggt tataccaaga aagcttcccc agttgcttcc cgacttatat
     3841 acagccaccg gattgccatt ggcaatattg tccaatgtca tattgacata atcatcgccg
     3901 agaataaggc gtacaaagct ttcattgtat atacttgaaa gcaccccgat gcccactgaa
     3961 agtccgaaaa caatagaggc aagtaaaaga tatcttcggt actccaccat aagcatcggc
     4021 acctctacct tgtaaaaatc aaccaatcga ttggaatccc ttctccgtgt gcgataaatc
     4081 ttctgaaatg cagcagcaca gagctgattg agatatgcca ccgttatgct cttaggataa
     4141 tatgtctgag cataggccaa atcattgacc aggctgacat ataggtcaga caattctccg
     4201 gcgtccacag ataaagatcc actggcaaat gcttcaaatg ccagccattt ttctttattt
     4261 tgttgtatga atgctgtttc cttcatttcg ttgtgaaaat aaatattaag tggaatatat
     4321 cactataaat acaactcaga atgttaatat taatttccct ttagcgggga ccggatcgcg
     4381 tattgctgca ttcttgatag acctggtgat aaaatatgta tatatcatca tagttattca
     4441 attacttagc cattcagatc ttgaattctc aatcgatcaa tggagcgcaa gtgccatcta
     4501 tattatttgc ctatcaccca tattctttta tagtttagtt tgggaatact tcaacaacgg
     4561 cagaactccg ggtaaaatgg tcgtcaaaat cagagtggcg aaaatagatg gttatcaagc
     4621 ggacttcatc gatatcttta ccagatgggc catgcgaatg atcgatattc agcttggctt
     4681 tgggttgata ggtctgatta cgatgacaag aaacaacaaa aatcaaagac tgggtgacat
     4741 agcagcaggt acttctgtaa tctcccaagc aaaaactttt aagtttgagc aaaccatctt
     4801 cgaagaatta cataaaaact atacaccaac ctatcccgat gtaatcaaac tttcagataa
     4861 cgatatccgc atcattaagg agaatcttga aaaatcaagg aaattcaatg atttgagcct
     4921 cattctaagg ttgaagtaca aagtagaaga tattttggga atcaaatcaa gtgaagatcc
     4981 ttatatcttc atcaacaagg taatcacaga ctataattat tatacccagg aatccgattg
     5041 atttccggac aattggatta gtaggctcat ttcaacacat acaccttgaa cacttcagtt
     5101 ggataatcgt ccccggttac atatacaaaa tacataccgg aaggccaatg agctgtgtca
     5161 acgaccaact ctgttgcaag gttatccttt ttatgaaata agcatatacc agtcgtgctg
     5221 catatccgga tatcaaattt ttgatgagct ttgttttcgg ccattctcaa tgtgacgtta
     5281 ccggctccat tatctgtaac aacttcaatc cgagaaaaat caggccaggt tattgcacga
     5341 accggtgaat aacttaccct tccattgatt tctgttattt caagacgata gtatactacg
     5401 gcagattcaa ttaccggtct gtcagtccat tgaaatgtac cttcacgccc ttctgtacta
     5461 aatactgtat taaaaagact tccgtcatta ctcatctgta cccgatatct cactacatcc
     5521 cgattacctt gccatttgag caatacttcc acacctcttc tgtctgcagc aaattcaagt
     5581 gcatgagtag caagtatgac agggtcacaa ccgggattta cggatagtcc taacacggaa
     5641 gctaacagat tgatataagc agcctgaaca tatatactca cctcggaaca ctcccaacta
     5701 ttttcattca tattggtaga ggtattccat cccgtgttcc aatctttata ggatttttgc
     5761 ggaggctggc ccgatggtgg ggagatcgaa ggaatagtaa aggatgcatt ggcccctcct
     5821 acaataaagc ccggaggagc agcatattga gatgtcaatg cattatcata gggtgtccca
     5881 tcaccaaacc atccatgata gatttcattg acacaggctt cggcatgcag gggatacata
     5941 ttggacaaat agacgatgcc caaaggatta ataccatgga aataatggag atattgctct
     6001 gccagttctt tatattggtc atggttaccc ggattgagat tgtaattcac gaaatcactt
     6061 tgctgtgcgc cgatataagc tttaacttcg ttgctacccc aatggtattg tgcattaggc
     6121 atatggcttc tgtataggtc tgtatttgag atatattcat tcaatcccat ccctgtatag
     6181 gtccctgcca atgaactcag aatattattt tttactgtag tacttgcccc gggcagagtg
     6241 gtataatata acattgctct gccaatagcc acattataag gcccccacca accattagca
     6301 ataggattca aggttgaata attattttcc acataggatt tataattatt attcccggta
     6361 gcggcataaa ggtatattgc agcagtcaaa gcatacaatc tctgtgtttc ggagtcccaa
     6421 tccgcatctc ctgatttgat gtccccatta tcacagctag tttgaaaatt ggtccatccg
     6481 gatgtggtta ctattgctct ggcccatgct gattgtgctc ttgcaagcat atcagcagca
     6541 aatgctccct gccccgtcac tcctgagtag atgcgatgag ctgcagcaaa gatggctgct
     6601 cccgcaatag tagatgaagt acattcaggg atgtaatatc ttgtacatac atcggccgat
     6661 ggcggactgt tgctacaatt gtagctatcg acgccaacct tgagaaatag tccgttggta
     6721 ccggtccctt cttgcatctt gaccaaccaa agcaattccc acttgacttc atcgaggata
     6781 tcagctatcc cattccccga ctccggaata ttattgtcat cgccaaatac ctgcgggaag
     6841 taataataac tatccatcag ggtgagcata ggtaatgcgg cgaaggtgac atacttattc
     6901 atatcaccgg catcaaacca tccaccactg acatcccttt ctgttgaagg gttgttttta
     6961 tccaaactgc ttctagcatg cttgtcctga ttgcttcctt cataagcagc tacatcatta
     7021 tactccggct tttcagtata tggcatcgat ttggcatagt tacagcgggc ataataataa
     7081 gtacggagca ctgccctcag attggcatca tacacattgg aaccgatgaa gaactggcca
     7141 cttcctactt gattgacatt gtcccatata taataaatac cgggatcagt aactgacgaa
     7201 aaatcgaaat accagccccg gtcgcccgat tgggtatgta cagccccacc attccacggc
     7261 gaaatagtcc cggacattat aacatctcca ccattccatt ttctgacttg ataggcacct
     7321 gttgaaatgc ccggactaaa ggactctgac gcattgaatc cattgatggg atctgaaatg
     7381 acggctatct tttttgagtt acatagataa ccaaactgat ccaccttgat aaatgggctg
     7441 atggtggcag gctgggcaga caaggatata aagtaaaggc atgcccatat tgatagaaaa
     7501 actctcatgg ctacaaataa aattaggaac aaaaaacaca cacgataaaa aaggggcgcc
     7561 gacaaagata aagaacattc tccttcaact taattttttt taaaaaattt tattcaatcg
     7621 ctgatggaaa aaatatcatt tattagtttt accaaaaaag aagatatatg tttcaaaata
     7681 tcaccaacaa acaaatttcc atgacaatta taattgtcaa aagttttttg gtgattgtat
     7741 tggcctggaa gctttgggag gcatcaaaag aaggatatca actttccatc gatagggagt
     7801 tttttacttt tttaatgatt ggatttcttg ctgaattagt tgatggctct ttggggatgg
     7861 cttatggagt catcagttcc agtttcctga ttttttttgg tgtttcaccg gtagtcgcct
     7921 cagcaggtgt acatacttca gaagtattca caaccggtgt ctctggtctt tcacatctgc
     7981 actttaaaaa tgtggataaa ggacttttct tccggattgt cattcccgga gtgattggct
     8041 cattaattgg agcgattgtt ttatccaaac tggtagatgg agagatcatc cgacctttca
     8101 tagcagtgta tcttttactt gtaggtatca aattgatagc acgggagatg caaggcgaca
     8161 aggtccatat caaacccctt aagtcagtgc ccattattgg ttttttcgga ggtgccttag
     8221 atgcaatcgg aggaggagga tggggcccgc ttgtcacttc tactatatta agcaggagcg
     8281 cctcgaatgc caaacacact attggcacgg tcaacacagc agaatttttt gttactttcg
     8341 ccagtaccgg tatatttatg ttcctgattg gaatttcttt ctttaatatt ttgctgggcc
     8401 tcattattgg tggtgtggtg gcagccccgc tcggcgcata cttcctgaag catgttcctc
     8461 acaaagtact gatgtatgtg gtgggctcgg tgttaattat agtttctgca ttttccatct
     8521 attattatat ctttaaataa gagaagacaa ttgtcagaaa ataaatcagg aatctcaaca
     8581 ggatacggaa agaggtatga acaatgttac cagaaatgtt aagctcatct ccaaataata
     8641 ttgcaggtgt cagcaattga aatccttacc tatggaatag ccagaaatta acaccttgct
     8701 tttgttttgc taaaaggaaa catttttctt attaagctat aaatatttac aataacccgg
     8761 acaattgcca tccggacatt tcatgttctt cgttgagaag caaaacctac ttagcgttgg
     8821 aaaatatgat ctttgttcta cgaaattcag aggcagtaag gactttagaa tgtggagtcc
     8881 tcctcaactt tcaggaacta tgagaaattg taatgggtgc aagcagatac aagtggtata
     8941 aaaaaaatcc cggaagctat ggccaccggg attctgtatc taataaaagt caggaatcaa
     9001 ttcatcacta catcgacaaa agtcctgtct ttctttcctt tcttgaatgc tacaacaccg
     9061 gaagtgagtg cgaaaagagt gtgatcctta ccgcatccga cattaacacc gggatggaat
     9121 ctggtaccac gctgacgtac gaggatatta cctgcgatta cagcctgtcc tccaaatttc
     9181 ttaacaccca atcttttgct tttactgtcc cgtccgttat ctgaactacc gacacctttt
     9241 ttgtgagcca tttcttaatt tttaaatgat taataaaaaa gattacccga ttgagtcaat
     9301 ctggagatga gtgaattgct gtctgtggcc attcttgaca cggtatccct ttcttctttt
     9361 tttcttgaag acgataacct tatctccctt gaccatttct gaaagtacgg ttgcagtaac
     9421 tgccacacca cttacagtag gagtaccgac tgccacatta ttgccatcac ttgtcaaaag
     9481 cacctggtca aatgtaagct tgtcgccctc ctttgcatcc aaccggtgga cgaagatctt
     9541 ctgacctcct tcaactttga actgctgacc ggctatagaa acaatagcga acatagttat
     9601 ttgatttaat ttttgataaa agtggggcaa aggtaaacat tattatctat tcaggcaaga
     9661 atttttatgc atttcatttc acgttctgaa aaataaatac ataaagcaac agtttccctt
     9721 gtattacaaa cattccccgg caatcatccg gatgttttac agcaaaccaa aaccatccaa
     9781 attccatccg gacaaaaatt ccaatccttc acaccataaa aaagttgaat atttttttat
     9841 aaaattttga atatttcaat atatacttta cctttgcacc cgcaaatcaa taaatctgat
     9901 aacaacatga aaaaggacat acatccttcc agctatagaa aggtagtttt caaggacatc
     9961 tctacggatg aggccttcat tactcaatca tgcgtcaata ccaaagacac aatcgtttgg
    10021 gaagacggta acgaatatcc actggtaaag gtggaaatat cttcattctc acacccgttc
    10081 ttcacaggaa agatgaagtt tgttgacacc gcaggacgta tcgataagtt caacaagaag
    10141 tttgcaaaat tcatgaaata agctgattct ttcagtccag aatatcaaaa agccatgtat
    10201 cccatggctt tttttatttg atttttctat cttgcatcat caaaagtccg caataaatga
    10261 aaatagtcct ttatgacggt cctgaatgga aagacctatt gcccctcact tttacaaagc
    10321 ccgttgccgg cctaaggata ggcattgata ccattcggga aaaatgggaa gcggcactga
    10381 ataccgaatg tctcatcaag acacaggact acttgcaggc tattcacccg gtggcaacct
    10441 ctgatgattt tctattcatc aatgcatctt acatccccca ccctgtcctg actaatcata
    10501 ttttgtcgat cagacataac caaagtttgg ctatcagcgg acaggtcata gcttatcgca
    10561 ctgactcatt tcatgtagcg tcttccgagg caaaacaatt tgacattata agtattaacg
    10621 cacctgagga tgttatacac atatgttatc catgggatat tttttccaac aacaaccggg
    10681 tcctgcaaca agattttgac agaataacgt caggtagg
//
LOCUS       DAOQMC010000059        10661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2394_length_10661_cov_4.653498, whole genome shotgun sequence.
ACCESSION   DAOQMC010000059 DAOQMC010000000
VERSION     DAOQMC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2394_length_10661_cov_4.653498"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PKV31_03790"
     CDS             <1..732
                     /locus_tag="PKV31_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNB92009.1"
                     /translation="KVIYNKLPLSVLLIVYNTPFILLGFRQVSKEFALKSIIAITGLA
                     IVIQFVHFPILTDDPLLIASFGGLFLGAGIGFAMRGGSVLDGTEVLAIYLSRKSMLSI
                     GDIILIFNIIIFLTAAYLLSIDIALYAILTYFVASRAVTYLIEGIEEYTGVTIISDNS
                     EEIREMLVHDLRFGVTLYSAKKGYGKRGHQSTEQIDVVYTVVTRLEISKLQDGVKAID
                     PSAFIIMSSVKDTVGGMIHRRGGVH"
     gene            1073..2848
                     /locus_tag="PKV31_03795"
     CDS             1073..2848
                     /locus_tag="PKV31_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB92010.1"
                     /translation="MDTTIEKFKGGEFLIKDYSFGSDYIPERMNEDQKMIRDTVREFV
                     EKELWTRGSKLEEQPALMDEAGELGLVGAHIPAEYGGQEFDTIINTILLEELGGGDAS
                     FSTTFAAHTGIGMLPILYFGTEEQKKKYLPELSSGKVRAAYCLTEPGSGSDALGAKTK
                     AILNDAGTHYLLTGQKMWISNAGFADVFIVFAQVDGDKFTGFIVEKGTEGMTLGEEEH
                     KLGIKGSSTRQIFFENAPVPKENVLGEIGKGHLIAFNVLNMGRFKLGVLVGGGCKVTT
                     TRAVNYAMEREQFKTPIGRFGAIQHKMAQMAINAYVLESTNYRIANLIEDWKASAIED
                     GVPYQQALLDAAEEYAIECAITKIYGSECIDYNVDELLQIHGGYGFSEEFVPAGLFRD
                     TRINRIYEGTNEINRLLLVNMLLRRVMKGQLDMVGPAWEVQKELASMPKMESFEGAYP
                     EETKALKNFKKILLMVAGAGAKYQMDGKVDLKEEQEILMNIADIMIDVFNAESVLSRL
                     KEVKNMDDGLLEEKEAMLKVFFHDAQDRIAKNAVDALASFATGDELKIMLMGVKRYSK
                     LEPVNVKNLRKKIAERIIEAGRYPF"
     gene            complement(2985..3701)
                     /locus_tag="PKV31_03800"
     CDS             complement(2985..3701)
                     /locus_tag="PKV31_03800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB92011.1"
                     /translation="MIDPQELKKRIVFEDNHLIAINKRPSWLVQGDKTGDMPLLDALK
                     DYIKKEYNKPRDIFLGSIHRLDRPVSGLVVFARTSKALERMNALFAGREVVKTYVAIT
                     EGKPPVSVGTLRHFLLKDNHNNTVNVVNPSRKGLKDVKEAILDYVEVNRHGLHTLIKV
                     VPHTGRPHQIRVQLASMGCPILGDVKYGARAPLSDQSIALHSFSLQFVHPVRKEALCL
                     VAPLPETAWWKPFDTDPLNK"
     gene            complement(3744..5690)
                     /locus_tag="PKV31_03805"
     CDS             complement(3744..5690)
                     /locus_tag="PKV31_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB92012.1"
                     /translation="MSRLFFITIALFYSLYLTAQDTDPNHIPCSKASAYRNPASLNNF
                     HNPLTENYDLKYYRFDWYIDPADYYIKGSATPYFEVLTNEFKEIYFDLSNQLQVDSVL
                     YHGLKINYSQPDQFVLKINFDVQIGIGTLDSLTIYYRGVPPAGGFGSFIQSKHGDNIP
                     VIWTLSEPFGAQDWWPCKNGLDDKIDSIDVFVTSPDQYRCASNGILVDEIRPGPGLMR
                     AHWKHRHPIAAYLVAIAVTDYTVYTDDVHLKDGTVMPMLNYVYPESLSKARKGTSDNV
                     RVLEFFDSLFVDYPFKKEKYGHAQFGWGGGMEHQTMSYVTGFDWGLLAHELGHQWFGD
                     HVTCGSWEDIWLNEGFATYLDGLSRERFFEPSYWYNWKFNLIKSITSRPDGSVWVANT
                     DDVSRIFHSRLTYNKGAYLVHMLRWKLGDEIFFKSLRTYLNKKGGGFARVADLKGILE
                     DVSGESLGEYFDDWFYGEGYPVYSVTWDSIHASPEQVLIRLDQTTSHTSVDFFEMPVP
                     IRLQGEGKEMTVRLDNTSSGQVFTIEPGFKVEKVEFDPDLWLLAKSSVAPGAVLADRE
                     VPEYGIQCFPNPAHNDLVINVNSLKFKGLSWRIIDVSGKLILKGELNAPYQRINIAGL
                     TAGAYRMLISDSQHVVSVHSFSRI"
     gene            complement(5812..6747)
                     /locus_tag="PKV31_03810"
     CDS             complement(5812..6747)
                     /locus_tag="PKV31_03810"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF009251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNB92013.1"
                     /translation="MEGIRSTLYNLPDGVTRYEGKVRDVFTRDNIFVSVASDRISAFD
                     HILPRSIPYKGQVLNQIAAYFLDSVRDIIPVWLDSTPDPNVSVGRKCTPIRIEMVVRG
                     YLSGHSWREYRSGKRFLCGVPLPEGLMENDPFPEPIITPSTKAMEGHDEDISKEEILE
                     RNLVDRQIYEEMEIKSRQLFERGTQMALKRGLILVDTKYEFGIYNGALTLMDELHTPD
                     SSRYFYLDTYFENQILHRPQNQLSKEFVREWLIANGFQGRTGDKMPLMDDSMVETVSK
                     RYIELYEKITGRDFIPASYDQVDERISRVLNGIFG"
     gene            complement(6826..7266)
                     /locus_tag="PKV31_03815"
     CDS             complement(6826..7266)
                     /locus_tag="PKV31_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92014.1"
                     /translation="MSIFDKAVLVIYRMAEKGLEVFLVKSEDEEIQSQLVEISEKFLT
                     DLPLRFSDNSKMIELDPIEDENMEKVRAVAIEGDWHDIPSLRAMLREDLQFMKVKMKK
                     YIPTIDSGNFILFKDAVKKAVRSEYRLLKELKDVLTDRNTINNI"
     gene            7747..>10661
                     /locus_tag="PKV31_03820"
     CDS             7747..>10661
                     /locus_tag="PKV31_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92015.1"
                     /translation="MNLRLLHILLFISISFVTNLDGQKLKSVGNQSEQNIEIRDCSTR
                     SPGTVSFGPIVGQSIDGRPDTIYLCYKDQIFIKNNGDGNFSTGDPNPGTPSAIGYLLY
                     GAPPTINGPDFNAIQGDAVLTNPPPPPGELWYFMDAPNGDITLYNDGSVNETFNSGKP
                     FPLYLAPVTFDAYYAAGGEYYGQWENGGSCVKVNTAAAFPVVYLNEIKINNFATNSPN
                     SLSGSFNVTGGFPEFRNGSTYSISMVKKGAPNVVANLFGGPFKHGDLVQFAVPSGGTY
                     ILTISDGKSCSVTKEIIMPQGNVSVLVQSGTVDLNDTICLEFTVKDFKDLLGGEFAIT
                     FNPLELKYVGLNFPQTNPLNLSPGGNFGLTEASNGYIVFAWTDANLNKKTLADGTVIF
                     SICFQAIGRPGTKTPVKVSPKRDGVIEFTDYDGTLYTVRTVDGIVTINNPTKLQVFFN
                     VCSTTGNTGSVTFTAYGPDAQYNYEFNNSGAPDFTNAGTPVTFSNLTPGPYKIDVYSI
                     SGVHVVHTVFIQNAPPINITGNLTNPTCFDTSDGNVQISLQGGVPPYAIEWSNFTFNT
                     NTVKDVGNGLYTVTVTDDIGCIFSQSFTLYTSPISINPPAVTDPACSGQKNGSITISA
                     SGGNPKTGNTYDFHWSYQNTSQIGVSIGSLTNIPEGTYYVTVTDNNGCNATSSFVVDP
                     GVVIDATVLKQLPVCFGDGKGRLTVTGTTNGTTSGPYTFIWSPNTGNVSSTSTTSIAS
                     GLNNGNYIVTVEDANGCFVKKELEINGPGEIKFLIANLKDDVCGTQPAGSALIYDGSG
                     NVTPFPDPNDPDNGFYRFTWSNGSNPTTVDAVSLNLTNLAGSASGTRYYVTITGADGC
                     TADTSFLIFKKDAPMITFDTLHSVSCAGGSDGSIRANITANGSDISTVTWSNNAGVPV
                     NSGDPKNVYTSTVNNLNGGQYIVTVVTLSGCIVTDTINLESAGSLVLDNNFIVPATCP
                     "
BASE COUNT         3266 a         2264 c         2131 g         3000 t
ORIGIN      
        1 aaagtgattt acaacaaatt acccctgtct gttttattaa tagtgtacaa tacgcctttc
       61 atcctactcg gtttcagaca ggtgagcaaa gaatttgccc tgaaaagcat catcgccata
      121 accggacttg caatcgtcat ccaatttgtc cactttccaa tactgacaga cgatccattg
      181 ttaattgcga gttttggtgg attatttcta ggagcaggca ttggctttgc catgcgaggt
      241 gggtcagtat tggatgggac ggaagtcctt gcaatctatt tgagcagaaa atcaatgtta
      301 tcaatcggag acattattct aattttcaac ataattatat ttttaactgc agcttattta
      361 ttgtcgatag acatcgcctt atacgccatt ctgacatatt tcgtcgcttc aagagcggtc
      421 acttacctga tcgaaggtat tgaagaatac accggtgtaa ccataataag tgataacagc
      481 gaagaaatca gggaaatgct ggtacatgac ctgcgcttcg gggttacttt atactctgcg
      541 aaaaaaggat atggcaaaag aggacatcaa tcgacagaac aaattgatgt agtctatacc
      601 gtagtgacac gtttggagat ctccaaattg caagatggtg taaaggcaat cgatccatct
      661 gcattcataa tcatgagcag cgtcaaagac acagtcggtg gtatgatcca cagacgaggc
      721 ggagtacact aacttttggt aggggaaaag gacatacctt cactatgaaa atgagaggag
      781 gtctaaggaa aaatccttat tataaaatta ctttttctaa tatttattct attctgttaa
      841 atattataca ttcatttaat taaaattcaa ctcggaatta atcactaatg tagactctca
      901 ttatactttg taaaatacaa gctaccgtat attattttgt ttattatata atatttcttt
      961 gttttcggta ttatgtattg cattgaaata gtcgattaaa taattttggc atttttttaa
     1021 ttatttacct ccaaaatgtg ttaactttac aaataagtaa tttaatcaac acatggacac
     1081 aactattgaa aaatttaaag gtggtgaatt tttaataaaa gattattcat tcggctcgga
     1141 ttacatccct gagcgcatga atgaagatca gaaaatgatt cgtgatacgg ttcgcgaatt
     1201 tgtagaaaag gaattatgga cccgaggctc aaaactggaa gaacaacctg cattgatgga
     1261 tgaagcagga gagttgggtt tagtaggcgc acatattccg gcagagtacg gcggtcagga
     1321 atttgatacc atcatcaaca caatcttact ggaagaactt ggtggcggag acgcctcttt
     1381 tagtacgacc tttgcagccc ataccggtat tggtatgctg ccaatcttat attttggcac
     1441 agaagagcaa aagaaaaagt atctcccgga attatcctcc ggaaaagtac gtgctgccta
     1501 ttgccttact gaaccaggat caggaagtga tgctttgggt gcaaaaacca aagctatttt
     1561 gaacgacgca ggaacgcatt acctgctaac cgggcagaag atgtggattt ccaatgcagg
     1621 atttgcggat gtcttcattg tatttgcaca ggttgatggg gataagttta ccggatttat
     1681 cgtagaaaaa ggaacagaag gcatgacact cggcgaggag gagcataaac tcggcatcaa
     1741 gggttcatcg acacggcaga tcttttttga aaacgccccc gtaccaaagg aaaatgtcct
     1801 gggagaaatt ggaaaaggcc acttaattgc attcaatgtg ctcaacatgg gaaggtttaa
     1861 gttgggcgtt ttggttggtg gaggctgcaa ggtcactaca acaagggcag tcaattatgc
     1921 tatggaaagg gagcaattca agacacccat cggtcgtttt ggagcgattc aacataagat
     1981 ggcacaaatg gcaattaatg cttatgtact cgagtctaca aactaccgaa tagccaactt
     2041 gattgaagac tggaaagcat cggctattga ggatggcgtg ccatatcagc aagcacttct
     2101 ggatgctgcc gaggagtatg ccatcgaatg cgccatcact aaaatatatg gaagtgagtg
     2161 tattgattac aatgtcgatg aattactcca aatccatggc ggatacggtt tctcagaaga
     2221 attcgtccct gcaggattgt tcagagacac acgaatcaac agaatctatg aaggaaccaa
     2281 tgagattaac cgccttttat tggtcaatat gctgctccgc agagtcatga aaggccaact
     2341 tgatatggta ggccctgcct gggaagtaca gaaggaactc gcttccatgc caaaaatgga
     2401 atctttcgaa ggagcctacc ctgaagaaac caaagcgttg aaaaatttca agaaaatttt
     2461 actgatggtt gcaggtgcag gcgctaaata tcaaatggat ggaaaagttg atctgaagga
     2521 ggaacaggaa atattgatga atattgccga tattatgatt gatgtattca atgctgaatc
     2581 cgtcttatcc cgcttaaaag aggttaaaaa tatggatgat ggtttacttg aagaaaaaga
     2641 ggcgatgctt aaagtattct tccatgatgc ccaggacaga atagccaaga atgctgtgga
     2701 tgcacttgcc tccttcgcta ccggtgatga gctcaaaatc atgctcatgg gggtcaagcg
     2761 ttatagtaag ctagaaccgg tcaatgtcaa gaacctacga aagaaaatcg ccgaaagaat
     2821 tattgaagca ggaagatatc ctttctaact gcagccagat aaagatgttt ttttaaggta
     2881 ttaagtttag attttacgtt gtagaaaact ccggaaattt ccggagtttt ttttatttaa
     2941 cgatgctgac cagcactata accatcaaaa tggatccaat gcttttactt attcaaggga
     3001 tctgtatcaa atggcttcca ccaggcagtc tcaggaagtg gagcgaccaa acacaacgct
     3061 tccttcctta ccggatggac aaattgaagg gaaaaactat gcagggcgat ggattgatcc
     3121 gacaaaggcg cccttgcccc gtacttgaca tctcccagta taggacaccc catagaagcc
     3181 agttgaaccc taatctgatg gggcctgcct gtatgtggga ctactttaat caaggtatga
     3241 agcccatgcc tgttaacttc tacataatcc aaaatagcct cctttacatc cttcaagcct
     3301 ttgcgggaag gattcacaac gttgacagta ttgttgtgat tgtctttcaa caggaaatgc
     3361 cggagagtac ctacggagac cgggggtttg ccttctgtta tcgcgacata ggtctttaca
     3421 acttcacggc cggcaaaaag agcattcatc ctctcaagtg ctttgcttgt acgcgcaaat
     3481 acaaccaatc ctgaaaccgg tctgtccagt cggtggatgc ttcctaaaaa aatatctctc
     3541 ggcttgttgt actctttctt tatataatcc ttcaaagcat cgagaagcgg catatcacct
     3601 gtcttatcgc cttgtacgag ccaagaaggc cgtttgttga tcgcaataag atgattatcc
     3661 tcgaagacga ttcttttctt taattcctga gggtcaatca cttttagaag atttatcttt
     3721 catattaccg gcccgtgaag caatcaaatc ctggaaaaag aatgcactga aactacatgc
     3781 tgagaatcac taataagcat cctatatgct cctgcagtca aaccggcaat attaattctt
     3841 tggtacggag cattcaactc tcccttaagg atcaacttac cggatacatc tatgatgcgc
     3901 caggataagc ccttgaattt aagagagttg acatttatga caagatcatt gtgggcaggg
     3961 ttcggaaaac attgaatgcc atattcaggc acttcccgat cggccagaac agcaccgggt
     4021 gcgactgaac tctttgccaa caaccacagg tcaggatcga attcaacctt ctccacctta
     4081 aaaccaggct caattgtaaa aacctggcca gaacttgtat tgtccaacct aacggtcatt
     4141 tctttgcctt caccttgcaa tcggatcgga accggcatct caaagaagtc aacagaagta
     4201 tgtgatgtcg tctggtcaag tctgataagg acctgttccg gagatgcatg aatactgtcc
     4261 caggtaacgg aataaaccgg atatccttct ccatagaacc aatcatcaaa atattcacct
     4321 aaactttctc cggatacatc ctccagtatt cccttcaaat ccgccaccct cgcaaagcca
     4381 cctcccttct tattcaggta ggtgcgcaaa cttttaaaaa agatctcatc cccaagtttc
     4441 caccttagca tatggaccaa ataagctcct ttattgtaag taagcctcga atggaagatc
     4501 ctgctcacgt catcggtatt ggccacccaa acggaaccat caggccttga tgtaatagat
     4561 ttgatcaggt taaacttcca gttataccaa taggaaggct cgaaaaagcg ctctcgtgac
     4621 aacccgtcaa gataagtagc aaacccttca ttcagccaaa tgtcttccca gctgccacag
     4681 gttacatgat ctccaaacca ttgatggcca agttcgtgag ccagcaaacc ccaatcaaag
     4741 cctgtaacat aactcatggt ttgatgttcc atacctccac cccacccgaa ctgagcatgt
     4801 ccatatttct cttttttaaa cggatagtcc acaaagaggc tatcgaaaaa ctccagtacc
     4861 ctcacattat ctgaagtgcc tttccgggct ttactaagag attccggata gacataattg
     4921 agcataggca taacagtgcc atcctttaag tgtacatcat ccgtatatac agtataatca
     4981 gtaaccgcaa tagccacaag atatgctgca atcggatgcc tgtgtttcca atgagctctc
     5041 atcaaacctg gtccgggcct aatctcatcc accagaatac cgttacttgc acacctgtat
     5101 tgatcaggag aggttacaaa aacatcaata ctgtcgatct tatcatctaa accattttta
     5161 cagggccacc agtcttgtgc gccaaatggt tcagaaagtg tccagattac aggaatatta
     5221 tctccatgct tggattgaat aaatgaccca aagcctccgg ccggcggaac tccccggtaa
     5281 taaattgtaa gactgtccaa agtccctatt ccaatctgta cgtcaaagtt tattttcaac
     5341 acaaactggt caggttgaga ataatttatt tttaatccgt gatataagac actatctacc
     5401 tgcaattgat tggacaggtc aaagtatatt tccttaaact cattggtcaa cacttcaaag
     5461 taaggtgttg cagaaccctt gatgtaatag tcagctggat cgatatacca gtcaaaacga
     5521 taatatttca aatcataatt ctcagtcaaa ggattatgaa aattattcaa agaagccgga
     5581 tttcggtatg cagatgcctt gctacatggg atatgattgg ggtctgtatc ctgagcggtc
     5641 agatacaagg agtaaaagag tgcgattgta atgaaaaaca gtctcgacat atctcaatat
     5701 ttaggaataa attgtccaaa tatatgccaa aaagggaatt taataaatag caactcctga
     5761 caacatttcg ttaatgttac caaaatcaca cacccaactc aaagtttagt attatccgaa
     5821 gataccgttg agtacccggc ttattctttc atcgacctgg tcataggatg cagggataaa
     5881 atctcttccg gttatcttct cataaagctc aatatagcgt ttactgacgg tttcgaccat
     5941 agaatcatcc atcaatggca ttttgtcgcc ggttcgaccc tggaaaccat tagctataag
     6001 ccattctctt acaaattcct tggaaagctg gttctgaggc cgatgaagaa tttggttttc
     6061 aaagtatgta tccagataaa aatacctgga actgtcaggt gtgtgcaatt catccatcag
     6121 agttaatgcg ccattatata tcccgaattc atattttgta tccacaagga tcaaaccacg
     6181 cttcaaagcc atttgggtcc ctcgctcaaa caactggcgg gatttaatct ccatttcctc
     6241 atagatttga cgatctacga gattcctttc cagaatttct tcttttgaaa tatcttcatc
     6301 gtgaccctcc attgccttgg tacttggtgt aatgataggc tcaggaaaag ggtcattttc
     6361 catcaagccc tccggcaggg gaactccgca aagaaatctt ttaccacttc tatattctct
     6421 ccatgaatga ccactgagat agccacgaac taccatctca atcctaatcg gtgtgcactt
     6481 ccttccgaca gaaacattcg gatcaggggt ggagtccagc cagaccggaa tgatatctcg
     6541 aacagagtcc aggaaataag ctgcaatctg attgaggacc tgacctttgt aaggaataga
     6601 cctcggaagt atatgatcaa atgcagaaat tcggtcagaa gcaaccgaaa caaaaatgtt
     6661 atctctggta aatacatccc ttactttacc ctcatatctg gtgacgccat cgggtaaatt
     6721 atataatgta cttcttatcc cttccataaa aataaattat ctgatccata atcgacatca
     6781 gccaagatag gctgattgta cactgacatc agatctactt aatgtctata tattattgat
     6841 ggtattcctg tcggtcagga catctttgag ttccttcaag agtcggtatt cggatctaac
     6901 ggctttttta accgcatcct tgaataaaat aaagttacct gaatcaatcg tcggtatata
     6961 ttttttcatc ttgaccttca taaattgaag atcttcacga agcattgctc taagtgatgg
     7021 aatatcatgc caatcacctt caattgcaac cgcccgtact ttctccatat tttcatcttc
     7081 aataggatcc agctcaatca tcttggagtt gtcagaaaag cgcaaaggca agtccgtcag
     7141 gaatttctct gaaatctcta ccaattgaga ttgtatctct tcatcttccg attttaccaa
     7201 aaaaacttcc aaacctttct cggccatccg gtatattacc aaaacagcct tgtcaaatat
     7261 cgacatatca actatttgaa aaattataat acaaagctaa acattaataa catataacaa
     7321 caaatatatt ttgatttttc atcactatcc taatcaaatc ttttgaatct ataaagtcca
     7381 ggcttaacta acccttaaat aaaaaagtaa tatctcaaac cgaattagag ctatgagtgg
     7441 cttaaaaaat caatgaaaat ctaataaaaa ttgcattcat ggtttttctg tattttggtc
     7501 gaatacagga tttaaatacg atggcaccca tataattcgt taaatttgta aataattttt
     7561 gtttcccaaa aaaagaaaaa caaatattaa aaatatatat attaattttg catcacatat
     7621 gaaagttgca ttcaagattg cagctaattg ataataacag atatatattt aataatgatc
     7681 aatttgggtt tttactttaa acatgcccag aaatcaggac gaactcaaca acacagaata
     7741 ttttgtatga acttaagatt actacacatc cttctattta taagcattag tttcgtcacg
     7801 aatttagatg ggcaaaagct taagtctgtc gggaatcaat cggagcagaa tatcgagatc
     7861 agggattgtt cgacaaggag ccctggcact gtaagcttcg gacctattgt cggtcaaagt
     7921 attgatggaa ggcctgacac gatatatctt tgttacaagg atcagatttt tataaaaaac
     7981 aatggcgatg gaaactttag caccggtgac cctaacccag gcactccttc agcaatcgga
     8041 tatttgttgt atggtgcgcc tcctactata aatggtcctg acttcaatgc aatacaggga
     8101 gatgcggtct taaccaatcc accaccgcca cccggggaac tatggtattt tatggatgca
     8161 ccgaatggtg acatcacctt gtacaatgat ggttcggtca atgaaacctt caactcaggc
     8221 aagccatttc ctttatatct ggcaccggta acctttgatg catactatgc agcgggtgga
     8281 gaatattacg gccaatggga aaatgggggt tcatgtgtaa aggtaaatac tgcagccgcc
     8341 tttccggtgg tgtatctcaa tgaaataaaa atcaacaatt tcgctaccaa cagtccgaac
     8401 tccctatccg gctcctttaa tgtgaccggt ggatttcctg aattcaggaa tggctcgaca
     8461 tattcgattt caatggttaa gaaaggagcg cccaatgtag tggcaaatct ttttggtggt
     8521 ccatttaagc atggggattt ggtacaattt gccgttccga gtggcggcac ttatatactg
     8581 actatttcag acggaaaatc ctgttcagtc acgaaagaaa ttatcatgcc acaaggcaat
     8641 gtatcagttt tggtacaatc cggcacagta gacctgaatg acaccatttg cctggagttt
     8701 accgttaaag atttcaagga tctgttaggc ggggaatttg ccatcacttt caatcccctg
     8761 gagttaaaat atgttggttt aaatttcccc cagaccaatc ctctgaattt aagtccagga
     8821 ggaaattttg gtctcacgga agccagcaat ggttatatcg tatttgcatg gacagatgcc
     8881 aatctgaata aaaagaccct ggctgatggt accgttatct tttccatttg ctttcaagct
     8941 attggcaggc caggaacgaa aaccccggtc aaggtttcac ccaaacgaga cggtgtaatt
     9001 gagtttacag attatgacgg cacgctctat acggtaagga ctgtagatgg gatagttacg
     9061 atcaataatc ccaccaaact gcaggtattc ttcaatgtgt gcagtacaac gggcaacacc
     9121 ggttctgtta cgttcactgc atatggacct gatgcacaat acaattatga atttaacaac
     9181 agtggagcac cagactttac aaacgcaggt acacctgtaa cattcagcaa ccttacaccg
     9241 ggaccatata agatagatgt atattcaatc agtggggtac atgttgtaca cactgtattc
     9301 atacaaaatg ctccaccaat caacattaca ggtaacctaa cgaaccctac ctgcttcgac
     9361 acttctgatg gcaatgtcca gattagtcta cagggtggag taccacctta tgcgattgag
     9421 tggtctaatt ttacctttaa taccaacacc gtgaaggatg tcgggaatgg actttacaca
     9481 gtcactgtta cagatgatat cggttgtata ttttcccaat cctttacact ttatacttct
     9541 cccatcagta ttaatcctcc ggcggtaact gatccggcct gttcaggtca gaagaatggg
     9601 tcgattacca tttctgcctc gggaggtaat ccaaagacag gcaataccta tgattttcat
     9661 tggtcatacc agaataccag tcagattggg gtatcaatag gctcattgac aaatattcct
     9721 gaagggacct attacgttac ggtaacagat aataacgggt gtaatgccac ctcgtcattt
     9781 gtggtagatc cgggcgttgt tattgatgcc acagtactca agcaacttcc tgtatgcttt
     9841 ggtgacggca aaggcagact tactgtcaca ggtacgacca atggcacgac ctcaggccct
     9901 tacactttta tatggagccc caataccggt aatgtctcaa gtacctcaac aacatctata
     9961 gcaagtggac taaacaatgg caactacatt gttactgtag aagatgcaaa tggctgcttt
    10021 gtcaaaaagg aacttgaaat taatggaccc ggggaaataa agtttctgat tgccaatctt
    10081 aaagatgatg tgtgtggcac acaacctgca ggttctgctt tgatatacga cggcagtggc
    10141 aatgtaacgc catttcctga ccctaatgat cctgacaatg gattttacag attcacatgg
    10201 tctaacggtt ctaacccgac gacagtcgat gcagtaagtc tcaatctaac taaccttgca
    10261 ggaagtgctt ccgggacaag atactatgtg actataacag gtgcggatgg ttgtacggct
    10321 gacaccagtt ttttgatttt caaaaaggat gcacctatga ttacttttga tacgttgcac
    10381 tccgtttcat gtgcgggtgg tagtgacgga tcgataagag ccaacattac cgccaatggt
    10441 tcagatatct ccaccgtaac ctggagtaac aatgccggtg ttcctgtcaa tagcggagat
    10501 ccaaagaatg tttatacctc gacagtcaat aatcttaatg gaggccaata catcgtcact
    10561 gtggttactt taagcggatg tattgtaacc gataccatca atctggagag tgcagggtct
    10621 ttggtactgg ataataattt cattgttccg gcaacatgcc c
//
LOCUS       DAOQMC010000060        10595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2415_length_10595_cov_5.526376, whole genome shotgun sequence.
ACCESSION   DAOQMC010000060 DAOQMC010000000
VERSION     DAOQMC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2415_length_10595_cov_5.526376"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..284
                     /gene="trxA"
                     /locus_tag="PKV31_03825"
     CDS             9..284
                     /gene="trxA"
                     /locus_tag="PKV31_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNB92016.1"
                     /translation="MGNDGLKVVDFWAEWCGPCKAIGPIIEELSKEYEGKATIGKLNV
                     DDNPEVSFKYGIRSIPTLLFIKNGEVVEKHVGAASRQVIASKIEANL"
     gene            443..1372
                     /locus_tag="PKV31_03830"
     CDS             443..1372
                     /locus_tag="PKV31_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB92017.1"
                     /translation="MSFFDTIDLKQHTNLELIARKVVEGFIIGLHKSPFHGFSVEFAE
                     HRLYQPGESIRNIDWKVYARSDKFFTKKYEEETNLRARIVIDISSSMHFPGKEVYNEV
                     RMNKLQWSALAAAALSYLLKKQRDAFGLTLFDEQPVYHSEAKSSSAHQQLIFSQLEPY
                     IYDQTFNKKTSAADSLHRIADMIHKRSLVIIFSDMLEDPARMEPMFDSLQHLKHNKHE
                     VILFHVVDASRELLFEFDNRPYEFIDLESGEKLRLQSNEIKREYADKMKQYEEELRLK
                     ALQYKIDFVSADINRDFDQVLQAYLIKRSRMRT"
     gene            complement(1741..2376)
                     /locus_tag="PKV31_03835"
     CDS             complement(1741..2376)
                     /locus_tag="PKV31_03835"
                     /inference="COORDINATES: protein motif:HMM:NF025028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HNB92018.1"
                     /translation="MKFLLLLLIFLIFIVIFAGCETGYVKKNGTWVWVTIDENYGKRN
                     HAIVGVDQGTFKVLKNKNYASDHMNVFFRGKKVNYADPKTFECLTFDDYSYAKDAGHV
                     YFRDAIIPGAEPSSFTTMQFPYSKDRKDVYCGNIPLRLSPEDVNTFKVTNEDKMMAGS
                     ISTMKLEHFLKYNPDYSWLAEMKDTMEMVITGESGTAVSQSKKFKGYKEVK"
     gene            complement(2390..2965)
                     /locus_tag="PKV31_03840"
     CDS             complement(2390..2965)
                     /locus_tag="PKV31_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92019.1"
                     /translation="MIFVDDVNNLVLGSVKLILASDWIHPGKYRMEILSRENIQHHIC
                     FTKRPDMLSSTDSGPLSEEKKEDQTPMEYRDGFGRTIKNSDLDMRKNAIEQTLGKIFR
                     SVEFVNQGKEGTVIYHEFSRTIKFYMELGGNDIVFYLNIPSASDWEHVTGFSLQERDS
                     IIEFVANETQKHQAPNCTYSIEEATIYFIHK"
     gene            3594..5201
                     /locus_tag="PKV31_03845"
     CDS             3594..5201
                     /locus_tag="PKV31_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNB92020.1"
                     /translation="MLRYLFLLCCFFSYDASARIFYIDPVNGSMDNDGSYDYPWRTLE
                     EVVAADLIQSYSYTPLPYDPNSSTLIIKNENGPVEPGDTLMLRSGMHGEFLLRGYINP
                     KMITIMADEGHKPILKQIRLQACKNWRFENLIISTEPYGYYLNDRLVFLETHGWHGPT
                     SGISILNCEIYSGEQPWETAEEWLANASSGIIIEGDSMIIENNYLKNVVMGITASGDH
                     IHAINNVIENFSADGMRMQGNDILFEGNTIMNCYDVDENHDDGIQSFIIGEGVFRNNI
                     IRSNIILNYVDQDQKLLGALQGIGCFDGFYENWLIENNVVIVNHWHGITLLGARNCTI
                     RNNTVLDPTPDIRPGGSWIMIDSHKDGRPSEGCIVANNVANSINANAQVNNNVVMDTY
                     EAYGSNFMDYKSFDFHLKEGSILIDSGSLQNAPETDIDGVVRPQGGGVDIGAYEWIVM
                     TSERHTIQNDIRIFPNPSTGHIHVEHLEGSGVVEVYDMRGHRLHQEKYTSQSLRLNLQ
                     DLKPGVYWIKIRSERIGTFHACRFIKL"
     gene            5755..6618
                     /locus_tag="PKV31_03850"
     CDS             5755..6618
                     /locus_tag="PKV31_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92021.1"
                     /translation="MKVKQIDRVELKRRGFISSFSGSSGIIYSDGFVYIIADKSNVLS
                     VFDVNDQTLKKEISLKADGSLEANVEKKYKPDFEAFSRHGNRYFIFGSGSAKNRFDLV
                     ILDERFDFVERLSIKNLYLAMMESTGIDRDNFNIEGVILEEDFALFFNRGNGPDRKNG
                     IIKVRNWLTDEPEVAGYSSIVLPDINGRHAAFSDAILHDGCICFLANGEDTQSVYDDG
                     AVAGSAFGMMDLSTFELLEFHIIDHSMKLEGLAVYKDLKEEHKMVFIVCDDNDFEHQT
                     IISQLTVFWRD"
     gene            7025..7882
                     /locus_tag="PKV31_03855"
     CDS             7025..7882
                     /locus_tag="PKV31_03855"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HNB92022.1"
                     /translation="MRNRSIACIFALFLGGIGVHKFYLGKVGQGVFYAIFSMTFIPVF
                     LGFIDFIRLATMSEDEFNRLYNDYIPFTTPKEWQTKHRYRNAKQDTVVFENRRNEPGR
                     DKDSFSQKERRKKRPMPLTANRAYKDGVKLLEDYRYREALEQFERARKASGDHPEILF
                     RMACCNAVLEQKAELLEHLQRAIKNGFKDFDRIRTTDALAYFRIQKEYEAIEVSGFVD
                     WPAISEDETEAVVETVEKTEVPNVEQSESVPLSTDELLFELKKLGEMRQNGVITEDEF
                     LKRKQKLYA"
     gene            7912..8577
                     /locus_tag="PKV31_03860"
     CDS             7912..8577
                     /locus_tag="PKV31_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB92023.1"
                     /translation="MFVNEIINQNIPCLEPEQTGEDGLRLMEEYMVRHLPVVKEDMVI
                     GLVSEDMIYDWDTNTPFSEYIFRGYPQVIHHDSHILEAVEVIVKNNLTCVPVTDRNFK
                     YLGLISLQDAFQALSMEFALDEPGAILVLEINRLDYSLTEISRIVESEKGAILSSFIS
                     HPLDSDSNLVHVTLKISLQDIQFVKATFERYNYKVVATFSSVEYIDTLAERYDALMHY
                     LNI"
     gene            9191..>10595
                     /locus_tag="PKV31_03865"
     CDS             9191..>10595
                     /locus_tag="PKV31_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNB92024.1"
                     /translation="MLPIKKADLTIEVGSRNISCFNLIDGIAWVTASGGTPPYTYLWS
                     NGATTDTIYNLPSNTYTITVEDFVGNKVAAEVYIAEPPLLHSGVLLSNPILDCTTTQL
                     TATATPIGGTPPCTFQWAFPQGVIDTGQTVNIVQPYNYVYTVTDSRGCTFIGDTTIGS
                     QNIPVVFLSLADTVSCNGLHDGSATSSVTGGAPPYTYLWSNGSTNPNLLDVGAGSYSL
                     TVTDTEGCSGQAYVFIPQPGALILSMDGNDVTCFGQTTGAIFTTLTGGSLPYSYLWSN
                     GETTPSLGNIGGGTYTLTVTDAHGCDVVGSVTINQGNEIHIYTNTFPVSCNGGSDGYA
                     TVFATGGAGGFQYNWSNGTSGSQATNLKAGTYLVYIIDKNQCVEERTLIITEPPLLEM
                     SLNSTPEILGGSDGTAWADVSGGTTPYTYNWSNGANTAIISGLTAGMYYLTVTDKKGC
                     LKLDSIEVAASNCAFSAK"
BASE COUNT         3139 a         2099 c         2364 g         2993 t
ORIGIN      
        1 agaaagcact gggtaatgac ggtttgaaag tggttgattt ttgggcagaa tggtgtggtc
       61 cttgtaaggc catcggtccg atcatcgaag agctctcaaa ggaatatgag ggaaaggcaa
      121 ccatcggcaa gctcaatgta gatgataacc ctgaggtttc tttcaagtat ggcattcgca
      181 gtatccccac cctgctgttt atcaagaatg gagaggtggt ggagaagcat gttggcgccg
      241 cttcccgaca ggtgattgct tccaaaatag aagccaacct ataaaattcg gcttagccag
      301 aagaaatatt cccttacgca gcaacgtttg tgtaagggtt tttttattgg aaggcgccgg
      361 aacagcgata tgatgtatag ataccccatg atgggagacc tctgtacacc ccaaaatact
      421 gacaacacat aacacatcga ccatgtcctt ttttgatacc attgacctga agcaacatac
      481 caaccttgag ctgatagccc gtaaggtagt ggaaggcttt atcatcggcc tgcataagag
      541 cccgtttcac ggcttttctg tggagtttgc cgaacacagg ctgtatcagc cgggagaaag
      601 tatccgcaat atcgactgga aggtgtatgc aaggtcagac aagttcttta ccaagaaata
      661 tgaagaggaa accaaccttc gcgcgcggat tgtcatcgat atatcctcct ccatgcattt
      721 ccccggtaaa gaggtataca acgaagtgcg gatgaataag ttacaatggt cggctcttgc
      781 cgccgctgca ctgtcctacc tcttgaaaaa gcaacgcgac gcctttggtc tcaccctttt
      841 tgacgaacaa ccggtgtatc attcggaggc caagtccagc agtgcacacc aacagcttat
      901 tttctcccag cttgagccat atatttatga tcagacattt aacaagaaga cgtctgctgc
      961 ggactctctg caccggatag cagatatgat ccacaagcgg tcactggtca tcatcttcag
     1021 cgacatgctg gaagacccgg cacggatgga gccgatgttt gattcactac agcatcttaa
     1081 acataataag catgaggtaa tcctcttcca cgtggtggat gcctcccgcg aattgttgtt
     1141 tgagtttgat aaccgcccct atgagttcat cgacctggaa agtggcgaaa aactacgctt
     1201 gcagtccaac gagatcaaaa gggaatatgc cgacaagatg aaacaatatg aggaggaact
     1261 acgtctcaag gccctgcaat acaaaattga ctttgtatcg gccgatatca accgcgattt
     1321 tgaccaggtg cttcaggcgt atttgatcaa gcggagcaga atgaggacat aaaagagaca
     1381 aggatacacg aagcttagag gacgagatag ggcgcaggac gattttcaaa ggacccaagg
     1441 aaaaacgcca acaatcagaa gagtccttaa caaaacagaa aaggaaatta tatcaattta
     1501 ggtcaaggca atccctttac tcacgtgcat gatatacatc attggcattg tttaagagag
     1561 aacacacaat gaggagtcat cagcgacttg taaaacttag caatacagac tgatacttcc
     1621 gggcaggcac cctcgtcagt atggtcagca actgtggcaa gagaagataa agccgaaatg
     1681 acttcatgct tttctgccat gtaaatatag gcgctaaaag ataagtgccg gagaaatcat
     1741 ctacttgact tctttatatc ccttaaattt ctttgactgt gaaactgcag tgccggactc
     1801 cccggtaata accatctcca tggtatcctt catttcagcc aaccaggaat aatcaggatt
     1861 atattttaaa aagtgttcca atttcatagt tgaaatagaa cctgccatca ttttatcttc
     1921 attggtaacc ttaaaggtat tgacatcctc cggggataat cttaagggaa tatttccaca
     1981 ataaacatcc tttctatctt tcgaataagg gaactgcatg gttgtaaaac tcgaaggttc
     2041 cgcaccggga atgattgcat cgcgaaaata tacatgcccg gcatcctttg catagctata
     2101 gtcatcaaaa gtcaggcatt caaaggtttt tggatcggca taattaactt ttttccccct
     2161 aaaaaataca ttcatgtggt cagatgcata gtttttattt ttcaacacct taaaggtacc
     2221 ctgatccaca ccaacaatag catggtttcg tttaccatag ttctcatcaa tggtaaccca
     2281 aacccaagtg ccattttttt tgacatatcc cgtttcacaa ccagcgaaaa tgactatgaa
     2341 aatcaggaag attaacagca ataacaggaa tttcattttg tcttaagttt catttgtgaa
     2401 taaaatatat tgtagcttcc tctatagaat aggtgcaatt gggggcctgg tgtttttgcg
     2461 tttcatttgc tacaaattca ataatagaat ctctttcttg taatgaaaaa cccgtgacat
     2521 gttcccagtc agatgcagag ggaatattta agtaaaagac aatatcattt cctcccaact
     2581 ccatataaaa tttaattgtt cgggaaaatt catgataaat gaccgtacct tccttacctt
     2641 gattgacaaa ctctacgctc cggaatattt ttccaagggt ttgctctatt gcattctttc
     2701 tcatatccag atcagaattt ttaattgttc tgccaaatcc atcgcgatac tccatgggtg
     2761 tttgatcctc ttttttttct tcagacaaag gtccggaatc agtggaagat aacatgtccg
     2821 gtctttttgt aaaacaaatg tgatgttgaa tattttcccg agacaatatt tccatcctgt
     2881 atttgccggg atgaatccaa tcacttgcaa ggattaattt cacactaccc aatacaagat
     2941 tattcacatc atcaacaaat atcatctcgc cattttcaat atttattatg ctgaccgata
     3001 aataatgtac atatgataca ggaagataaa caataatttg ttgacaatcg gagtaatggg
     3061 tagttgaaac atgttcaagg gaaaaattat tcaaaccctc cgtccctatt ggtccggcgc
     3121 taatattatc aacccggtca atttcgatct gacccggata tttagtatta tcatccataa
     3181 tacaccaaca gcaaccttgt acaaaaatag tgataatccc gaacctgaat tataatccgg
     3241 atatctctga tgacactttt tcagctatat ttagcggtag tggtgtgtgg ctcctaaaaa
     3301 gtattggcag atatagttgt ttataagtat tgggatagat accagttaat ttttactttt
     3361 agcttgataa gcaagtacat tcgtaataat cgggtttcat tgccttgttc aatatggtag
     3421 agaggtacac ggccagttct aagggaacgt aatttcggtg ggtatttgat ttaatttgta
     3481 tagtatgtat tacaacaaac ggacgtattt tcttaccgaa tcgttgtaaa tgtgttatat
     3541 tgcatggaag atgtagtggc aatatagttt tagtttaact aaccattttt ccaatgttac
     3601 gatacctctt tctgctctgt tgtttttttt cgtatgatgc ctcagcccgt atcttttata
     3661 ttgatccggt aaatggcagt atggataatg atggttcgta tgattacccc tggcgcaccc
     3721 tggaggaagt ggtggcggcc gaccttattc aatcctattc ttatacaccc ctgccttatg
     3781 acccgaatag cagtacctta atcatcaaga atgagaatgg ccccgttgag ccgggggaca
     3841 cgctgatgct tagaagcggc atgcatggag agttcttgct tcggggctac attaatccaa
     3901 aaatgatcac catcatggct gacgaaggtc ataagccaat ccttaagcag atccgacttc
     3961 aggcctgtaa gaactggcgc tttgaaaacc taattattag taccgaacca tatggatatt
     4021 atctcaatga caggctagtt tttctggaga cgcacggatg gcacggaccg acatccggaa
     4081 tcagcatcct gaactgtgag atatatagcg gggaacagcc ctgggagact gcagaagaat
     4141 ggcttgccaa tgccagtagc gggatcatca tcgaagggga ttccatgatt attgaaaata
     4201 attatttgaa aaatgttgtc atgggcataa cagcctccgg cgatcatatc catgctatca
     4261 acaatgtcat tgaaaatttt tcagccgacg ggatgaggat gcaaggcaat gacatcctgt
     4321 ttgaaggcaa caccatcatg aattgctacg atgtcgatga aaatcatgac gacgggattc
     4381 aatcctttat tattggagaa ggtgtattcc gaaacaacat catccgctcc aacatcatcc
     4441 tcaactatgt cgatcaggat cagaaactcc ttggcgcact tcagggaatc ggctgctttg
     4501 acggatttta tgaaaactgg ttgatagaaa acaacgttgt cattgtcaat cactggcatg
     4561 gaatcacttt actgggagca cgaaactgca ccatccgcaa caatactgtg ttggatccga
     4621 ctcccgatat acgcccgggg ggctcctgga ttatgataga cagccacaaa gatggcaggc
     4681 cgagtgaagg ttgtatcgtc gccaataatg tcgccaattc catcaatgca aatgcgcagg
     4741 taaataataa tgtggtgatg gacacctatg aggcgtatgg ttcaaatttt atggattaca
     4801 aaagttttga ttttcatctg aaggaaggca gcattttaat tgattcaggg tcgttgcaaa
     4861 atgctccgga aacggacatc gatggggttg tccggccaca aggaggtggc gtggacatcg
     4921 gcgcttatga atggattgtt atgacttcag aaagacatac aatacagaat gatatcagaa
     4981 tatttcccaa cccctcaacc ggtcatatcc atgtagagca tcttgaagga tcaggggtag
     5041 ttgaagttta tgatatgcgg ggacataggt tgcatcagga aaaatatacc tcacaatcac
     5101 tccggttgaa tctgcaagac ctgaagccgg gagtctactg gatcaagatc aggagtgaga
     5161 ggattggaac ctttcatgcc tgtcgtttta taaagcttta gaaataagcg aaggaggatt
     5221 agatatatta tgatttaggc cttgaattta cctcaaaagt tgattgataa tggcttttaa
     5281 tcaaagtgtg taaacaggca taacatatcg ttgatatggg tcgttgctgt aaaatttcct
     5341 tttaaatgga taaatcctta tccccctatt taaagaatat tcattccatt cattgtacct
     5401 acagcggcaa aacacagcca tttcaaagcc gtcccggaaa tattcgctcc gggcgtatga
     5461 tttagaagtg atggtgatat tgcagctatt attgtttaaa aaatggattg gtgattgaaa
     5521 tattttgact tctttcctcc aagaagcata ggcactctac atgtccacct cagacggaag
     5581 aaagtgaaaa cctatttatt tgtatcgttt ttggcaaaaa aatattttgg cagactccat
     5641 accggagagg agctatcagg ctgaaacaaa gaagtatcca tgcggttaga aaatgcagtt
     5701 ttagttccgg aaattttaat cagacagtta gaaaaacaca taattttgac cgctatgaaa
     5761 gtaaaacaga ttgacagagt agaattaaaa agaagaggct tcatttcttc cttcagtgga
     5821 agttcaggga ttatttattc cgacgggttt gtctatatta ttgctgacaa atccaatgta
     5881 ctatcagtct ttgacgtcaa tgatcaaacg ctcaaaaagg aaatcagcct taaagcggat
     5941 ggatcgctgg aagctaatgt ggagaaaaag tacaagccgg attttgaggc tttctctcgt
     6001 catggcaatc ggtattttat ctttggttcg ggatcggcaa aaaacaggtt tgatctggtc
     6061 atacttgatg aaaggtttga tttcgtcgaa cggctatcca tcaagaatct ttatctggca
     6121 atgatggaat cgaccggaat tgaccgggac aactttaata ttgagggtgt aatattagag
     6181 gaagattttg cccttttctt taatcggggt aacgggcctg atcggaaaaa cggaataatc
     6241 aaagtcagaa actggctgac cgatgagccg gaagtggctg gttattcaag tatagtgttg
     6301 ccggatatca atggccggca tgcagctttt tctgacgcca tcctccatga tggctgtatc
     6361 tgtttcctgg caaacgggga agacacgcaa tcggtgtatg atgacggggc ggtcgcggga
     6421 agcgcattcg gaatgatgga tcttagtaca tttgaacttc tggaatttca catcattgat
     6481 catagtatga agctggaggg gcttgcagta tataaggatc tgaaggaaga gcacaaaatg
     6541 gttttcattg tatgtgacga taacgatttt gaacatcaaa ccatcatctc ccaacttact
     6601 gtgttttggc gggattgatt ggtcagaatt aagccggcaa ttacggactg atcacatttt
     6661 cgatgaaaca ctaaaaatcc ggcaataagg taatcataaa attacctgtt atataactca
     6721 tacatatcat ttctttaaat gttccgatca ggtagatcga aaatgtcaga tcgggtcatc
     6781 acaatcccga ttgctttttc ggagctttcg ttcataacaa aatcggcttc cttgtttccc
     6841 ctcaccggtt atttttttta agctcctgaa ttcgattgtc tgaaaatact ttaattttat
     6901 cgaataaaaa gcgatatttg gaatattcta ttgtccttta ttgttgaaaa aagaaatagc
     6961 caggtaattt tacaacaagc tgtatttatt gaaaaagatg aatgtaccaa ataatacata
     7021 tccaatgaga aacaggagta tcgcgtgcat atttgccctg tttttaggag ggataggtgt
     7081 ccataagttt tatcttggca aggtaggaca gggcgttttt tatgcaattt tttccatgac
     7141 ttttatcccg gtcttcctgg gttttatcga ttttatacgc ctggcgacaa tgagcgagga
     7201 tgaattcaac aggttgtaca atgattacat tccgtttact acgccaaagg aatggcaaac
     7261 aaagcatcgt tacagaaatg cgaagcagga tacggtcgtc tttgagaatc gccggaacga
     7321 accgggcagg gacaaagaca gtttcagtca aaaagaacga agaaaaaagc gaccgatgcc
     7381 gttgaccgcc aaccgggctt ataaagatgg tgtgaagttg ctggaagact ataggtatcg
     7441 ggaggctctg gagcagtttg aacgtgcacg aaaggcaagc ggggatcatc cggagatact
     7501 ttttaggatg gcatgttgta atgcagttct ggagcagaag gcggaattgc ttgagcacct
     7561 ccaacgggcg attaaaaatg gattcaagga ctttgacagg attcggacaa cggatgctct
     7621 ggcatatttc aggattcaaa aggaatatga ggccatcgag gtgtccggct ttgtggactg
     7681 gcctgctatc agtgaggatg aaacagaggc ggttgtcgaa acggttgaga aaactgaggt
     7741 gccgaatgtg gaacagagcg aatcggtccc gttgtcaacc gatgaattgc ttttcgaatt
     7801 gaagaagctt ggagaaatgc ggcaaaatgg agtgattacc gaggatgaat ttttaaaaag
     7861 gaaacaaaag ctctacgcgt gacgttttag tataaataat actgattgca aatgtttgtc
     7921 aatgaaatca taaaccagaa catcccctgt cttgagccgg agcaaaccgg tgaagacggg
     7981 cttcggctta tggaggaata catggtcagg catttaccgg tggtaaaaga agacatggtc
     8041 attggattgg tatctgaaga tatgatctat gattgggata ccaacacacc attttccgag
     8101 tacattttca gagggtatcc tcaggttatt catcatgaca gccatattct tgaggccgta
     8161 gaagtgattg tcaagaacaa cctaacctgt gtgccggtga ccgacaggaa ttttaaatat
     8221 ctgggattaa tatctcttca agatgcattt caggcactaa gcatggagtt tgctcttgat
     8281 gagccgggtg cgattctggt actcgagatc aacaggctgg attattctct gaccgagata
     8341 agccgcatag ttgaatcgga aaaaggagcc atcctctcca gttttatttc gcatccgttg
     8401 gattcagata gtaacctggt ccatgttacc ttgaaaatca gcctgcagga tatacaattt
     8461 gtcaaagcca cttttgaacg ctacaactat aaggtggtag ccaccttttc cagtgtggaa
     8521 tatatcgaca cgctggctga gcggtatgat gctcttatgc attaccttaa catttaattt
     8581 gcggaggctg tttatttcga taaatatgat gcctgcggga atttttttac aaaaaaaaga
     8641 atttaaatat aacccggtca ttcataaggg atggttgcat ttatgtggcc tgattggcca
     8701 ttttgatatc taccggaacc ggagaacctg ccggtcagtc atctgcaccc tttccatggt
     8761 ttaggaggag aataattaag ggtatataat atttgattgg tcacctgctt tcatcaattg
     8821 attccaccgt tgccctttta atattgtaag ttctgatcga tacgtacatt atggaaatgg
     8881 gacacagcgg tcttagcgag gtataatttc aggtgtcatc aggtactttg cctgaatttt
     8941 tggaacagaa attgtatgta taattatgaa ttgtaatata tagaggatgg aacacaatat
     9001 tgtattcggt ggtattgcaa taaatattta attatcaagg attaataaat tgaaataatt
     9061 tgtttttgat atcgggtaaa cgaaaagaac aacgatgaga gaatgctaca ctaaaacatt
     9121 ttttcttaaa aatgtaatta ttttaacttt tctgggattt ttttcacacc agataaaggc
     9181 caacggacct gtgcttccaa tcaaaaaagc cgatttgacc atagaggttg gttcgcgtaa
     9241 tatcagttgc ttcaatctta ttgatggcat agcgtgggtt acagcttcag gtgggacgcc
     9301 tccgtatact tatctatgga gcaacggagc caccacagat actatatata accttccatc
     9361 caacacgtat accataacag tggaggattt tgtcggtaat aaggtggctg ccgaagtgta
     9421 tatagcagag ccgccccttc ttcactccgg tgtgttgttg tccaacccga ttctggactg
     9481 cacgactact cagctgactg cgacagccac acctataggt ggcactcccc cctgcacatt
     9541 tcaatgggca tttccacaag gggtgattga taccggacag actgtcaata tcgtgcaacc
     9601 ttataactat gtatatacag tgacagattc cagaggttgt acttttattg gagataccac
     9661 gattggatcg cagaatattc cggtcgtttt cttatccctt gcagatacgg tcagttgcaa
     9721 tggtttgcat gacggaagtg cgacctcatc ggtaacaggt ggcgctcctc cttataccta
     9781 tctttggtcc aatggatcaa ccaatccgaa tctccttgat gtcggtgccg gatcatactc
     9841 tctcacggta acagatacag agggatgttc ggggcaggca tatgtattca taccgcaacc
     9901 cggagcgctt atattatcta tggatggcaa tgatgttacc tgctttgggc aaacaacagg
     9961 agctattttc acaacgctga ccgggggctc attgccctat tcctatttgt ggtccaatgg
    10021 agagacaaca ccatcattgg gcaatatcgg agggggaact tatacgctta cggtaaccga
    10081 tgcgcatgga tgtgatgtgg tcggatcggt gactatcaac cagggtaatg aaatacatat
    10141 ttataccaat acgtttccgg ttagttgtaa tggtggaagt gacggttatg caacggtttt
    10201 tgccacagga ggagccggag gatttcagta taactggtct aacggcactt caggatcaca
    10261 ggctaccaac ctaaaagcgg gaacatatct tgtatatatc attgataaaa accaatgtgt
    10321 tgaagaaaga acactgataa tcacagaacc tccacttctg gagatgagtc tgaacagcac
    10381 cccagaaata ttgggtggca gtgatggaac cgcatgggcg gatgttagtg gcggtacgac
    10441 gccatatact tacaattgga gtaatggtgc caacacggca attatttccg gtctgactgc
    10501 cggcatgtat tatctgacag tgacggataa gaagggatgt ctcaaactgg attccattga
    10561 agtggcggcc agcaattgtg ccttctcagc gaaac
//
LOCUS       DAOQMC010000061        10519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2438_length_10519_cov_4.522458, whole genome shotgun sequence.
ACCESSION   DAOQMC010000061 DAOQMC010000000
VERSION     DAOQMC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10519
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2438_length_10519_cov_4.522458"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="der"
                     /locus_tag="PKV31_03870"
     CDS             complement(<1..1121)
                     /gene="der"
                     /locus_tag="PKV31_03870"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNB92025.1"
                     /translation="MSNIVAIVGRPNVGKSTLFNRLIGKKQAIIDDVSGVTRDRQYGE
                     AVWNGKSFIVVDTGGFVQGSDDVFESAIRDQVRIAVAEASAIIFIVDVETGETDLDEK
                     VANMLRQSGKNVILVVNKVDNSNRYIEAQEFWGMGFKHVHFISSMTGSGTGELLDELI
                     PMIGEAEQLDTDLPKIAIIGQPNVGKSSYTNVLLGEERNIVTEIAGTTRDSIHTRYNL
                     FNKDFYIIDTAGIRKKQRVNEDLEFYSVMRSIRAIEEADVCALLVDAQTGIEAQDMSI
                     FRLVQKRNKGIAIFVNKWDLVEKETNTARDFEKAIKAKLAPFNDVPVVFISALEKTRI
                     FKSIEVLLRVFDNKIKKIATSKLNEIMLPIIEQTPPPAYR"
     gene            1257..1913
                     /locus_tag="PKV31_03875"
     CDS             1257..1913
                     /locus_tag="PKV31_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB92026.1"
                     /translation="MSKPKIQVLVVDDHKMFVEGVQAIFANSPEIHIAGTIFDGKEVM
                     AAIENTSGLDIILLDINLPNINGLELTRMISKKYPNIKILILSMYNNAEYIREVLKEG
                     ASGYVLKNTDHEELLNAIQSVYAGNSFYSQSVTQTMMNSFAKKQQGTNMDIMQVKISK
                     REKEILGLIIKEYTAQEIANMLFISLHTVETHRSNLMSKLGVRNSAGLVRVALEHGLV
                     "
     gene            1930..3801
                     /locus_tag="PKV31_03880"
     CDS             1930..3801
                     /locus_tag="PKV31_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB92027.1"
                     /translation="MAELGCNIILIKNKLLSLLPVLMIVSVTYYPSWGNHENLLLTDS
                     LKNSLITGLNSIKKSGSDEQKASFYQKLAEYYEGQSRQDSAISYYQKSSIHYNQAGND
                     IMYYEILAKLGSLNYNVFNYKFAIEYFREVTDYYKRKKMYLPYVKAMNQLGLAYSKIN
                     DDINARQCFIATLEINNNILRDTHLILENKLLIIQNDIKTGNLDRALSLANHNERVAK
                     RYGDRNLLAQTLFQLGRINFKSDNFQKAETYLHIADSMMVETGSLQALPELYKILSQT
                     YIELGKKEMLLPTLEKYYSVVDKIKNTEIVKSSQEIAQRFDSVNKDQMIKQLENENEL
                     KTINSRQQRIMIYILIFAFLATILAIYLIYRGYTSRLKSNQIIARQKSELQDKQLKQI
                     EQEGQIKAMESMLIGQEAERNRIGKDLHDSLGAMLSTIKLQMSADANKNGSIDSPAMK
                     SAKEMIDEACEEVRKISRDMMPITLSKYGLHTALEELVDKYTFEGGPTVIYQSFGFNR
                     VSDKDLDLFVYRIIQELINNSIKHAEAQEIIVQINYLEDLMMITVEDDGKGFEYNSTE
                     YSGMGLKNVEYRTQYLKGKVTFDSGPGSGTIVVVEIPVTQFVKNKEVISGQTDPPRV"
     gene            3952..4524
                     /locus_tag="PKV31_03885"
     CDS             3952..4524
                     /locus_tag="PKV31_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HNB92028.1"
                     /translation="MKHLLLYLSLIAVISCKTIEPNRSQKRVERRNERKANKGILDKE
                     MANTMVALADSRLMGISCSKLAQKKAGSEYYTTYASHAMENETFFLKEIKSISKSNNI
                     SLPRKIGAENEIKYDELKALSNKSFDKKYRKIEKKRLKEDRKLLNRLSASDDPIIREF
                     ATKWSAPVNEWRSNTEGTFVIAQFHWSIRL"
     gene            complement(4608..5408)
                     /locus_tag="PKV31_03890"
     CDS             complement(4608..5408)
                     /locus_tag="PKV31_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92029.1"
                     /translation="MKDKNQWIEVVAFALPLLIASSLFFSCKQDKLVNDWTRMNLSGY
                     VMTIKEHSFKAIDTLGEIVQGERMSPSWRRDSYIVFNRAGNKVEENFYRNDGKMWSKS
                     VFSYDKNRKKIEEVVYRADRALWYTKITRFDSAGHIVEEEMFKPDNSLGVRMTFVYDG
                     SGNKIEEWKYTDNGKLSIRSVFKYDKNRNKIEEIMYNSEGGQIVRWVSKYDAKGRQIE
                     EDYYNAKNQLEARETYQYQFDSKGNWIQQIIFEDDSPKYVIRRELIYY"
     gene            complement(5798..6418)
                     /locus_tag="PKV31_03895"
     CDS             complement(5798..6418)
                     /locus_tag="PKV31_03895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HNB92030.1"
                     /translation="MERREMLKLIALYTGAAMIGGEFLLAGCKAPTGEDITFSDKVKS
                     LLNEVGETILPRTDTPGAKDADVAKVMEVVVRDCYWPEHQKVFVEGIRQIDEEAKGRF
                     GKTFMEISPEQRKELLIALEEEAKKFNEKVEEKDKPVREKMDKESKGYDFIASPRHYY
                     TMMKQLTMLAYFSSKECLTEAFEYIPVPGRYDGAYKIKPGDKLFSG"
     gene            complement(6428..8107)
                     /locus_tag="PKV31_03900"
     CDS             complement(6428..8107)
                     /locus_tag="PKV31_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNB92031.1"
                     /translation="MADNIYDAIVIGSGIAGGWAAKELAEKGLKVLMLERGRNIEHIK
                     DYVNANKDPWVFEHRGERTREMINTHPVLKRDYPLNEKVLDMWVNDLESPYIEDKRFD
                     WYRGYHLGGRSLLWGRQSYRWNKWDFEANGKEGIAVDWPIRYEDLAPWYSYVERFAGI
                     QGSYEALEVLPDSEFLPAMEMNIVEKDLARNLKEKYKGSRHMFIGRSANITKPMPEQN
                     RVNCQYRNKCWLGCPYGAYFSTQSSTLPAAKATGNLSLEVNKIVTRLLYDKDSKKATG
                     VETLDAINNQTYEYKAKIIFVCASAFNSTWILMNTATDIWPDGLGSSSGELGHNVMDH
                     HFRVGAGGSVEGYEEYIEYGRRSTGIYIPRFQNIYGDKRNYLRGYGYQGGAGRGRGVP
                     VAEMLGAEFKDAQFKPGKWYMGITAFGEMLPYHENTIKLDKEKKDKWGLPVLRMDVEL
                     KENELNMRKDMMAEGKAMLEAMGVKDAYEYDWGYAPGMGIHEMGTARMGRDPKTSVLN
                     GNNQVWDAPNVFVTDGACMTSASCVNPSLTYMAMTARAADFAVKELKKMNL"
     gene            complement(8282..9034)
                     /locus_tag="PKV31_03905"
     CDS             complement(8282..9034)
                     /locus_tag="PKV31_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB92032.1"
                     /translation="MNNLQLLLVEDEVIIAENIRQSLVDLGYECVTVCNTFDDAIEVI
                     EKGEIDLAIMDINLDDAEGRTGLDLASRLKKVKPTPFIFITAYSDKETVESASKLGPS
                     AYLVKPVNDKTIFSTIQIAMQNFNSNQTPVHSDSPESPAQYFFTKIGNKTFKIFWSEV
                     YLIRAIKNYVEIKAFGYTQTFLIRTSLTNCLESILPDNQKNYFVRINRAEAIRSEIVR
                     SYDNQKIYTNYGEFKLSSDHVIELQGKMRNIN"
     gene            complement(9031..>10519)
                     /locus_tag="PKV31_03910"
     CDS             complement(9031..>10519)
                     /locus_tag="PKV31_03910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB92033.1"
                     /translation="LKSSTTAIIDSKVYYDSAALIAHGLADSSLLATNYLSLGRMYYD
                     WGTQPDMAIEYIEEAGKLFEEDKDYENHYYESKLLLCKSLLERSESSKVLTILEQLLE
                     KISNSSLDMTTLSGIYLDLGMVALMCHDTILVKKALQGIEILKLKQPDLAVPDPMKYY
                     LLHSEAGFFAKEDNLQPLDSLDKLYSTLGIMQDSLFYAGRLTSLMERSGRFEKAFHYQ
                     TILTNLLLHENARYDKLAKQKIEFAERSLDHTRNIEKKTDQLWVIIICWLVLFAIASS
                     YIIIFLRRGSTKYYKLSKDLEFANNNAAILYKELHHRIKNNLHLIFSLLQMQERKTDD
                     KVTKLNLRDARLRIESIAIMHEEMMNLDGKIDFKDFIFRLIQLIINCFSLSTHIISEI
                     NFEKVEIPHKYNFPIALIINEWISNSIKHAILGDKYLEITLNVFQENDQVVIEYYDNG
                     DQKAETEPSSGLGTNIVQLLMKQIKAELFKGHRGLYHYKIKMGRI"
BASE COUNT         3154 a         2378 c         1917 g         3070 t
ORIGIN      
        1 cgatatgcag ggggtggcgt ctgctctatg atgggtagca tgatttcatt aagctttgaa
       61 gtggcaatct tcttaatttt attatcaaat acgcgaagca aaacttcaat tgatttgaaa
      121 atccttgttt tttccaaagc tgaaatgaat actacgggca catcgttgaa tggagccaat
      181 ttagccttga tggccttctc aaaatcgcgt gcagtgttgg tttccttttc tacaagatcc
      241 catttgttga caaagatggc gatgcctttg ttcctttttt gaacaagacg aaaaatactc
      301 atatcttgag cctcgatgcc agtctgagca tcgacgagta aggcgcagac atcagcttct
      361 tcaatagcac ggatagagcg catcacggaa taaaattcaa gatcttcatt gacacgttgc
      421 ttttttcgaa ttccggccgt atctatgatg taaaaatcct tattaaatag attgtatctt
      481 gtgtgaatag agtccctcgt cgtacctgca atctccgtta cgatgttgcg ctcttcgcct
      541 agcaagacat tggtgtagct tgactttccg acattgggct gaccgatgat ggctatttta
      601 ggcagatccg tatccaattg ctccgcctcc ccaatcatgg gaatcagttc gtccagtaat
      661 tcaccggttc cactcccggt cattgaacta ataaagtgga catgcttaaa tcccatcccc
      721 cagaattcct gcgcttcgat gtaacgattg gaattatcca ccttattgac caccaggata
      781 acattttttc cggattgcct caacatgttg gcgacttttt catccaggtc agtttcaccg
      841 gtctccacat ccacgatgaa tatgattgcc gaagcctcag caaccgcaat tcttacctga
      901 tcacggatcg cactttcaaa cacatcatcc gaaccctgaa caaagcctcc ggtatctact
      961 actataaatg acttaccatt ccagactgcc tccccatatt gcctgtcgcg tgtaacacca
     1021 ctcacgtcat caatgatggc ttgtttctta ccgatcagac gattgaacag cgttgattta
     1081 cctacattcg gacggcctac tatggctact atattactca ttaaaaatat ttatcaaagt
     1141 gcgaaagtat aataaattaa gcgaattaat atatgagaat caaaaatttg acgtaatttg
     1201 gcacttattt aggcctttcc ctgtccaata ttatactcaa atagttgatt tgatctatgt
     1261 caaaaccaaa aatccaggtt ctcgtggttg atgaccataa aatgtttgta gagggagtac
     1321 aggcaatttt tgccaattct cctgaaattc atattgcagg gactatattt gatggaaagg
     1381 aagtcatggc agccattgag aatacatcag gactggacat catcctcctg gacatcaatc
     1441 ttcccaacat taacgggctg gaacttaccc gaatgatcag taaaaaatac ccgaacatca
     1501 agatactgat cctgagcatg tacaacaacg ccgaatacat cagggaagta ctcaaggaag
     1561 gagcatcggg ttatgtcctc aaaaacaccg accacgaaga attactgaat gccattcaat
     1621 ccgtatatgc cggcaattcc ttttacagtc aatccgttac tcagaccatg atgaatagtt
     1681 ttgccaaaaa acaacaaggt acaaatatgg atatcatgca ggttaagatt tcaaaacggg
     1741 aaaaagagat cctcggcctg atcatcaaag aatataccgc tcaagagatc gctaatatgt
     1801 tgttcatctc actgcatacc gtggagacgc atcgcagcaa tctaatgtcc aaactgggag
     1861 tccgcaatag tgcaggactg gtcagagtgg ctcttgaaca tggattggtt taatcagtga
     1921 tgggacgata tggcggaatt gggctgtaat attattctta taaaaaacaa gcttctctcg
     1981 ttgttgccgg tattgatgat tgtttcggtg acctactatc cctcatgggg taatcatgaa
     2041 aatttacttt tgacggattc gttgaaaaat tcactcatca caggattaaa ttccatcaag
     2101 aaatcgggaa gtgatgagca gaaagcatca ttctaccaaa agctcgctga atattatgaa
     2161 gggcagtcgc gccaggacag tgccataagt tattatcaga agagctccat tcattataat
     2221 caggccggaa atgatataat gtactatgaa attctggcaa aactaggatc tctgaattat
     2281 aatgtattca actacaaatt tgcaatcgaa tatttcaggg aggtaacgga ttattacaag
     2341 agaaagaaga tgtatctccc ttatgtcaag gcgatgaatc aactggggct ggcttatagt
     2401 aagatcaatg atgatatcaa tgccaggcaa tgttttattg ccacacttga aatcaataat
     2461 aatatcctca gggatacgca cctaattctc gaaaacaaac tgcttatcat tcaaaatgac
     2521 atcaagacag gcaatcttga ccgtgcacta agcctggcca atcataatga aagggtcgca
     2581 aagaggtatg gcgacaggaa tcttctcgct caaacgcttt ttcaactggg gcgcatcaat
     2641 ttcaaatccg ataattttca aaaggctgaa acctatctac atatagccga ctccatgatg
     2701 gtagagactg gcagtcttca ggcattgccg gaattataca aaattctttc tcaaacctac
     2761 attgagctgg gtaagaagga aatgctgtta cctacactgg agaaatatta ttccgtcgtg
     2821 gataaaatca agaataccga aatcgtcaag tcaagtcagg aaattgccca acggttcgat
     2881 tcagtaaaca aggaccagat gatcaaacag cttgaaaatg aaaacgagct taaaaccatc
     2941 aactcccgcc agcaaagaat tatgatctat atcctgattt ttgccttcct ggccacaatc
     3001 ctggctatct acctgatata cagaggatat accagcaggc taaaatccaa tcaaatcatt
     3061 gcccgtcaaa agtctgaatt acaggacaag caactcaaac aaatagagca ggaaggccaa
     3121 atcaaggcca tggaatccat gctcattgga caggaagccg aacgcaacag aataggaaag
     3181 gacctccatg atagtcttgg agctatgttg agtaccataa aattgcaaat gagtgcggat
     3241 gccaataaga atgggagtat agatagcccg gcaatgaaaa gtgcaaagga aatgatcgat
     3301 gaagcatgtg aggaagtgcg aaagatttcc agagacatga tgccgatcac cttatccaaa
     3361 tacggtctgc ataccgcctt ggaggaattg gtcgataaat ataccttcga agggggaccc
     3421 actgtcatct atcaatcatt cggcttcaac cgggtcagtg ataaagatct tgatctcttc
     3481 gtgtatcgta tcatccagga attgatcaat aacagtataa aacacgcgga agcgcaggaa
     3541 atcattgtac aaatcaacta tctggaggat ctgatgatga taacagtcga ggatgacggc
     3601 aaaggatttg aatacaactc cactgaatat tcaggtatgg gactgaaaaa tgtcgagtac
     3661 cgaactcaat atcttaaggg caaagtaact tttgattccg ggccgggctc gggtaccatt
     3721 gtcgtcgtcg aaattcctgt aacacaattt gtcaaaaaca aagaagtcat ttccggacag
     3781 acagatccac ctcgagtgta aggacacaca gctatcaagg tatttatacc cggcaataag
     3841 tgttacaatg ttttgaatac tcctgattga tttgtttcca ttcttacaat tttcaaggat
     3901 aagaaacaat aattgaacac tttcggggtt tattatacaa accattgtaa agtgaagcat
     3961 ttactcctat acctgtcatt gattgctgtt ataagctgca aaaccattga acccaacaga
     4021 tcgcaaaaac gtgtagaacg aagaaatgag cgaaaggcaa acaaagggat actggataag
     4081 gaaatggcca ataccatggt ggcattagct gattcaaggc ttatgggaat aagttgctcc
     4141 aaactggcac aaaaaaaagc aggttcggaa tattatacga cttatgcgag ccatgcaatg
     4201 gaaaatgaaa cgtttttcct gaaagaaatc aaatccattt caaagtcaaa caatatatca
     4261 ttacccagaa aaatcggcgc tgaaaatgag atcaaatacg atgagttgaa ggcactgtcc
     4321 aacaaaagtt tcgataagaa ataccgaaag attgaaaaga agagattaaa ggaggacagg
     4381 aaacttttga acaggctatc agcttccgat gatccaatca ttcgtgaatt tgctacaaaa
     4441 tggagtgccc ctgtcaatga atggcgcagt aataccgaag gtacattcgt tatagcccaa
     4501 tttcattggt ctatacgact gtaacctcgg cattatacca aaaatttatg gactattata
     4561 taaatcatct tggtataacc taaaacaaaa ataaatagct tattgggtta ataataaata
     4621 agctccctgc ggatgacata tttgggcgag tcatcttcaa aaataatctg ttgaatccaa
     4681 ttacccttag agtcaaattg atactgataa gtttccctcg cttccagctg attcttagca
     4741 ttgtagtaat cttcctctat ctgccgaccc ttggcatcat attttgagac ccatctcacg
     4801 atctgtccac cttcggaatt atacatgatt tcttcaatct tattcctgtt cttgtcatac
     4861 ttaaaaacgg atctgatact caattttccg ttgtccgtat atttccactc ctcgatctta
     4921 ttccccgatc cgtcatatac aaaggtcatt ctgaccccta atgaattatc gggcttgaac
     4981 atttcctctt ccacaatatg tccggcactg tcaaatctgg taatcttggt ataccataaa
     5041 gcgcggtcag ccctatacac tacctcttct attttttttc tgttcttgtc atacgagaac
     5101 acgcttttgg accacatttt tccatcgttt ctgtaaaagt tttcttccac tttgttcccc
     5161 gctcgattaa atacaatata agaatcacgt ctccagctgg gactcatacg ctctccctga
     5221 acaatttccc ccagggtgtc gatcgccttg aatgaatgct ccttaatggt catgacatat
     5281 ccggaaagat tcatcctggt ccaatcgttt actaatttgt cttgcttgca actaaagaac
     5341 aaactgcttg ctatgagcag aggcaaagca aaggcaacaa cctcaatcca ttgatttttg
     5401 tctttcattt aagcttactt aaaatcaggc aaccgaatat tatcctattt cttactgaat
     5461 taataattaa aaatattgca ttgatatttg aatcctgaag caaatcttat accattatta
     5521 catataagcg ccaaacataa taaattgatt tgtcattttc aagacgttca tcaatatttt
     5581 ctaaaaatca tgaccccgtg ccggcatact gataaacaaa tgtggattca aacctgttca
     5641 aaatgggagg caatgattcc ggttgacagc attcaacatc ctcgcgaaga caaagtaaag
     5701 acctacggct aaaacattag tagagtactg acgatgagat acttacatat caaataaaat
     5761 aaacattact gacgtaaaca ttaaatcatg aaaaacatca gccgctaaat agtttatcgc
     5821 cgggttttat cttataagct ccgtcataac gacccggaac aggaatatat tcgaaggctt
     5881 cggtgaggca ttctttcgag ctaaaataag ccagcatggt cagttgcttc atcatcgtat
     5941 aataatgtcg tggagaagcg ataaagtcat accctttgct ttccttatcc atcttctccc
     6001 gaacaggctt atctttttcc tccacctttt cattgaactt cttagcttcc tcttcaaggg
     6061 caataagcaa ttccttccgt tgttcagggc ttatctccat gaatgtctta ccgaatctgc
     6121 ctttggcttc ttcatcaatt tgcctgatgc cttccacaaa cactttctgg tgttccggcc
     6181 aataacaatc acgtaccact acttccatga cttttgccac atcagcatct ttggcaccgg
     6241 gagtatccgt acgaggaagg atagtctcgc ctacctcgtt gagcaacgac ttgaccttat
     6301 cagaaaaggt gatatcctct ccggttggcg ctttacagcc cgccaaaagg aattcaccgc
     6361 ctatcatggc agcacctgta tatagcgcta tcaatttcaa catttctcgt ctttccatag
     6421 ccttgtccta taaattcatt ttctttaatt cctttactgc aaaatcagca gcccgtgctg
     6481 tcattgccat gtaggttaat gaaggattga cgcatgaagc tgaggtcata caagccccat
     6541 cggttacaaa aacattgggt gcatcccaaa cctggttgtt accattaagt acagaagtct
     6601 ttggatccct ccccatgcgg gcagttccca tctcatggat acccatgccg ggagcataac
     6661 cccaatcata ttcataggcg tccttgacgc ccatagcttc cagcatggct tttccttcag
     6721 ccatcatatc cttccgcata ttaagctcgt tttcttttaa ttcgacatcc attctaagca
     6781 ccggaaggcc ccatttatct tttttctcct tatccagttt aatggtattt tcatggtatg
     6841 gcaacatttc tccaaaagcg gtgatgccca tataccattt gccaggcttg aattgtgcgt
     6901 ccttgaactc agcgcccaac atctcagcca cgggtacacc acgaccgcga ccagcacctc
     6961 cctgatatcc atatccacgg agataattgc gcttatctcc ataaatgttt tgaaatctgg
     7021 gaatatagat gccggttgaa cgacgtccat attcaatata ttcttcatat ccttcaacag
     7081 agccgcctgc tccgactctg aaatgatggt ccatgacatt atgccccaat tcgccgctac
     7141 tactgcccag accatccggc caaatatccg ttgcggtatt catcaggatc catgtagaat
     7201 tgaaggctga ggcacacaca aagattattt tagccttata ttcataagtc tgattattga
     7261 tcgcatcgag cgtctcgacc ccggttgcct ttttgctgtc tttgtcataa agcagcctgg
     7321 taacgatttt atttacctcc aaactgagat taccggtagc ttttgctgca ggtagtgtgg
     7381 aggattgagt agagaaatat gctccatatg gacagcccag ccaacattta ttgcgatact
     7441 gacaattgac ccggttctgt tcaggcatag gcttggtgat gttggcactc cgcccaataa
     7501 acatatgccg tgagccctta tacttttctt tgagattgcg ggcaagatcc ttctccacga
     7561 tattcatctc catggcgggc aggaattcac tatccggcaa tacttccagt gcttcataag
     7621 agccttgaat acctgcaaaa cgctccacat aactatacca tggcgcgaga tcttcatacc
     7681 ggatcggcca gtcgacagca atgccttcct tgccattcgc ctcaaaatcc catttattcc
     7741 agcgataact ttgtcgtccc cacaacagag aacggccgcc cagatgatat ccacgatacc
     7801 aatcaaaacg tttatcctca atatatgggc tttcgaggtc attgacccac atatccagaa
     7861 ctttttcatt caacgggtaa tcacgcttaa gcaccggatg tgtattgatc atctccctgg
     7921 tgcgctcgcc acgatgctca aacacccagg gatccttgtt cgcattgaca tagtccttga
     7981 tgtgctcaat gttacgccct ctttcaagca tcaacacttt gagccctttc tcggccagct
     8041 ccttagctgc ccatccgcca gctattccag agccgatcac tatcgcatca taaatattat
     8101 ccgccattcg ccatttgatt taatttccca aatttagtat aaaaattctt ctgccagaca
     8161 aaaaataatg atatcgatgt ccactattct taaaataaaa ccacgaattg gccttattgc
     8221 caaacaaacg ccccatcttc caaaaccttt ctatcaaaaa caatgtcaga aaatagccaa
     8281 ttcaattgat atttctcatc ttaccttgca actcaatgac atggtctgag ctaagtttaa
     8341 attcaccata attggtataa attttttgat tgtcgtaaga tctgactatc tctgaccgga
     8401 ttgcttccgc gcgatttatc ctaacaaaat aatttttttg attgtcagga aggatggatt
     8461 caaggcaatt ggtcaaagag gtccttatta aaaaagtctg agtatatcca aaagctttta
     8521 tctccacata atttttaatg gcccttatca ggtacacttc cgaccaaaat attttaaaag
     8581 tcttgtttcc aatcttggta aagaaatact gtgccggcga ctctggtgaa tctgaatgga
     8641 ccggagtctg gttggagttg aagttctgca tagcaatctg aattgtacta aagatggtct
     8701 tgtcattgac cggcttgacc agataagcgg acggtcccag cttagaagca ctctcgacgg
     8761 tttccttatc actatatgcc gttatgaaaa taaaaggtgt aggttttact ttcttcaatc
     8821 tagaggcaag atcaagccct gtccttcctt cggcatcatc cagattgata tccataattg
     8881 caagatcgat ttcgcctttc tcaatgactt ctattgcatc atcaaaagta ttacataccg
     8941 tcacacactc atatccaaga tccaccagac tctgacgaat attctctgca ataatcacct
     9001 catcttctac taacaacaac tgaaggttat tcatatccta cccattttta ttttataatg
     9061 ataaagacct ctatgtcctt taaacaattc tgccttgatt tgtttcatca atagttgcac
     9121 aatatttgtt cccagcccgg aagatggctc tgtttctgcc ttttgatccc cattgtcata
     9181 atactcgatt actacctggt cattttcttg aaaaacattc aaagttattt ccaaatattt
     9241 gtcacccagg attgcatgtt taatgctgtt ggaaatccac tcattgataa ttaaggctat
     9301 cggaaaatta tacttatgtg gtatttctac tttttcaaaa ttaatttccg aaataatatg
     9361 tgtgctcaat gaaaaacagt tgatgatcag ctgaataagc ctgaaaataa agtctttgaa
     9421 atcgatctta ccatccaaat tcatcatctc ctcatgcata atggcaatac tttctattct
     9481 tagtctggca tctctgaggt tgagttttgt taccttatca tccgtctttc tttcctgcat
     9541 ctgcaacagg ctaaaaataa gatgcaggtt attcttgatc ctatggtgga gttccttgta
     9601 aagtatggcc gcattgttat tggcaaactc cagatccttt gaaagtttat aatacttggt
     9661 cgaacctctc cttaaaaaaa taatgatgta agaagacgca atggcaaaca ataccagcca
     9721 acatatgatt atcacccaca gctgatctgt ttttttctca atatttcttg tatgatcgag
     9781 tgatcgctcc gcaaactcaa tcttctgttt cgccagtttg tcataccttg cattctcatg
     9841 taagagcaag ttggtcaaga tagtctggta atgaaaggct ttttcaaacc tccccgacct
     9901 ctccattaga ctagtgagcc ttcctgcata gaaaagacta tcctgcataa taccgagtgt
     9961 actgtacaac ttatccagtg aatccaacgg ttgaagatta tcttcttttg caaaaaaacc
    10021 ggcttcactg tgtaaaagat agtacttcat cggatcaggg acagccaggt ccggttgctt
    10081 caatttcaat atttcaatac cttgaagcgc ttttttgacc agtattgtgt cgtgacacat
    10141 caatgccacc atgcccaagt caaggtaaat tccggacagg gttgtcatat caagactgct
    10201 atttgaaatt ttctccaata attgctcgag gatagtcagg actttgcttg attcactcct
    10261 ttccaagagt gatttacata acaataactt tgattcataa taatgatttt cataatcttt
    10321 atcctcctca aacagtttcc cggcttcttc aatatattcg atagccatgt caggttgtgt
    10381 tccccagtca taatacatcc tacccagaga aagataattg gtggccagta agcttgaatc
    10441 agccaggcca tgagcaatca aggcagctga atcataatat accttactgt caataatggc
    10501 tgttgttgat gatttaagc
//
LOCUS       DAOQMC010000062        10492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2445_length_10492_cov_4.516528, whole genome shotgun sequence.
ACCESSION   DAOQMC010000062 DAOQMC010000000
VERSION     DAOQMC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_10492_cov_4.516528"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..438
                     /locus_tag="PKV31_03915"
     CDS             55..438
                     /locus_tag="PKV31_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB92034.1"
                     /translation="MIDELSELAEFQGKTIEMEISGKFKHTMDHELASILVVNLVKNA
                     IIHSLRGTHITIRIFDQQFSVENEGAHGPLPDEQIFTHFFKTENSAPGLGLGLAIVQS
                     ICRHEQFKIEYSFEGKHKFTVTGTK"
     gene            793..1242
                     /locus_tag="PKV31_03920"
     CDS             793..1242
                     /locus_tag="PKV31_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92035.1"
                     /translation="MKIKWIYIIALLLMAPGYAWSQKLSSGEVPSIVLNNFKLAYPKA
                     YDVSWKKSGESYSAEFCVEKKCNLKVWFNKYAQLTKEMEELVFNTVPDRIKTKVKENY
                     GKFKIDEITKETVGKTTKYILKLESKFEDLKIIFNEDGKELSKEEDD"
     gene            1335..1769
                     /locus_tag="PKV31_03925"
     CDS             1335..1769
                     /locus_tag="PKV31_03925"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNB92036.1"
                     /translation="MKKILFLAFMISTISLIGCDDKETLLDADKIPAEIKTYVEKHFP
                     GKNILQCIKDVDGLEVSYDVVVEGGYKLEFNRDKKIDSVKGVSKLPDSVVPEKILAYV
                     ASNYPGNYIIEWDADKTTQEVKLDNRLELVFNSAGDFLRIDD"
     gene            complement(2105..3307)
                     /locus_tag="PKV31_03930"
     CDS             complement(2105..3307)
                     /locus_tag="PKV31_03930"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNB92037.1"
                     /translation="MAYSESLSQSAFYRYLIPAFLILLSFTGCSQNSVSGHIVLSDVD
                     FKPVVYLLEPTGFSSLVSSYECKVVDSAEINEAGDFRFDKFSAPEKEGIFLLAIQKKG
                     EKYLNRLENNHPDSGNYIPFLLKKGYPVEIRSESGAFLRNGQIASTLEGNRDILVLIA
                     NRSRLFKQFLSDQAEVNEENLLENEKSLYNFQQQLVESVMGSQDLYVLALALQWAGIK
                     GDYERIPELVKATCKGMSAIEPEHVWTQQVCKKSGGLPLTPGDAFPDFMMPMTDGDTI
                     SLRSLLGSQLTIIDIWASWCAPCRKENRNTLVPLWDNFHDSGLQIIGYALESERVVWL
                     NAIKRDGADRWPHASHMEGDTSPFLDLLKITTIPANYILDRNGVILAKNLHGKELEEW
                     VTGKLGKE"
     gene            complement(3362..4396)
                     /locus_tag="PKV31_03935"
     CDS             complement(3362..4396)
                     /locus_tag="PKV31_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HNB92038.1"
                     /translation="MIRFNIYPFLSILLMFLMSCCNNQSGVNQLEQYFAYGDSIESAG
                     VKMIPIHTPYGDFRVWTKRFGTNPDVKVLLLHGGPAMTHEYMECFETFFQRQGFELYE
                     YDQLGSFYSDQPENDSLWTIQRFVDEVDQVRQAIGADSNNFYLIGNSWGGLLAMEYAL
                     KHQDHLKGMVVSNMMASIPEYIQYFNNTLAPMMDPGALAEIKALEASKDYTNPRYEEL
                     LQEHYYNLHLCRLPEWPEPVTRSFSHGNNHIYVLMQGPSEMGIPPEARLYGWDIKSRL
                     HEITVPTLMVGARYDTMDPGAMEEQSKLVQRGRYLYCPNGSHLAMWDDQKVFMDGVIR
                     FIKDVDEGKM"
     gene            complement(4422..4667)
                     /locus_tag="PKV31_03940"
     CDS             complement(4422..4667)
                     /locus_tag="PKV31_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92039.1"
                     /translation="MVNMCNLPGVIGVLFTLCPVTFGGGAFSGITFIILILSFKRKCH
                     FSGYFEGFLAAFDHHSHGSECIETVRILRATEPKRNH"
     gene            complement(4754..6778)
                     /locus_tag="PKV31_03945"
     CDS             complement(4754..6778)
                     /locus_tag="PKV31_03945"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNB92040.1"
                     /translation="MEMQDSEFARAIRQGIPSTLPEPVLRDPLVAHAPVRNLIGVLTP
                     EERKLAIANALRYFQPEWHPVLAPEFADELDTYGRIYMYRFRPQYEMKARPVEEYPAL
                     SPQAAAIMLMIQNNLDYKVAKYPHELITYGGNGAVFQNWAQYLLTMKYLSEMTDEQTL
                     ALYSGHPMGLFPSHKEAPRVVVTNGMMIPNHSTKNDWNKYNALGVTQYGQMTAGSFMY
                     IGPQGIVHGTTITLLNAGRLMNLGDTLRGKVFVTSGLGGMSGAQALAGIITHAVTVLA
                     ETDGDAIRQRTDDGYILKENIYADLEACVARMQSARSSEEVITLVYHGNVVDLWEYFV
                     IHNIDIELGSDQTSLHNIDDLGYTPVGYTFEEAKALMLRDHKKFREAVSASLIRHVAA
                     INALHDRGMYFWDYGNAFLKEAGVAGADVFDHDKKSGYRYPSYVEDIMGPLCFDFGFG
                     PFRWVCLSGLQEDLDMSDQIAGDVITSLMKTSAKDIMPQLQDNLQWIRNAKEQIPVVG
                     SKSRILYADAEGRIAIARAFNDAIRSGVLKGPVVLGRDHHDVSGTDSPYRETSNIYDG
                     SSFTADMAVHNVIGDSFRGATWVSLHNGGGVGWGEVINGGFGMVLDGSPEAERRLTMM
                     LHWDVYNGIARRSWARNPHAMSTIQRARDLYPHMVITEVNLVDSKVLDNL"
     gene            6920..7513
                     /locus_tag="PKV31_03950"
     CDS             6920..7513
                     /locus_tag="PKV31_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92041.1"
                     /translation="MKNTLLLTTILSFILIVPAIGQKVKSEGNLDFLKGVKEVTLAYK
                     YDKMAVGKYKDGNEYVAKKTKELNEKEKGKGDDWAKKWVSDRSERFEPSFENLLSEYC
                     KGIDFDQNNKSEYTMTLETVFTEPGFNVGVMRQNAYINVIVHFTDKAGKELATLSIDK
                     IPGRDVFGYDFDTGQRIEEAYSKAGKTIGKYLTKKVE"
     gene            complement(7717..8655)
                     /locus_tag="PKV31_03955"
     CDS             complement(7717..8655)
                     /locus_tag="PKV31_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNB92042.1"
                     /translation="MRKIATIESIGSSTRIEGATLTDQEVKDLLKDLKITELKTRDEQ
                     EVIGYHEVLELIYSNYSEIKLSENYIKQLHQMLLKYSNKDERHRGTYKFLSNKVMATY
                     PAGEQRTIFATTDPALVASEMKELVGWTNETLKQKTIHQLIVIGSFIYDFLSIHPFQD
                     GNGRLSRLLTTLCLLQNDYSFIQYISFENHIEQNKKAYYEALMNGQKCRGTDNERIDL
                     WLIFFLESLKTLTEKLEKKYDVFKSKGGYLNERQKLIKDFIMSNQPNKVSDLANHFPE
                     IRLSTLKKDLQYLRVEQVLIMIGKGKGSVYVLTEKE"
     gene            complement(8656..8790)
                     /locus_tag="PKV31_03960"
     CDS             complement(8656..8790)
                     /locus_tag="PKV31_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92043.1"
                     /translation="MDKFIQKLNFDFVTNQQIIQLIGYIDGFKGKWNIAEKQENRYLK
                     "
     gene            complement(9457..10284)
                     /locus_tag="PKV31_03965"
     CDS             complement(9457..10284)
                     /locus_tag="PKV31_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92044.1"
                     /translation="MKWDFIKGIYEPFIGIDSFCFAFTKKGIHHSGLLCSGISNENLW
                     EMILFYGKCRHGEYIKMLSDIRIKAYQNEGGFSKMSKFANMVEKNLYIIAGCNGADKT
                     TASFTILPEIIECKEFVNADEIAKGLSPFQPEKVAFEAGRIMINRINELLINGETFAF
                     ETTLSTRSYRQKIETAKKKGYTTTLLFFWLKNVDLAKERVITRVLEGGHNIPPDVIER
                     RYLKGIQNLFNLYLPIVDGAFIFDNSYGYPELIAQQTLGEKVEIINTEKYNELKSYL"
BASE COUNT         3105 a         2269 c         2024 g         3094 t
ORIGIN      
        1 aactttttga tcgatggatg cgggtaaaac cactttgaaa ttatttttaa caatgtgatt
       61 gatgaattat cagaattggc agagtttcag ggcaagacaa tagaaatgga aatttccgga
      121 aaattcaaac acaccatgga tcacgagctg gcatcaattc tggttgtcaa tctggtcaaa
      181 aatgccatta ttcattcact acgtggaact catataacca tccggatttt cgaccaacaa
      241 ttctcagtgg aaaatgaagg agcacatggg ccactgcctg atgagcaaat atttactcat
      301 tttttcaaaa cagaaaattc agctcccggc ctcggacttg gattagcaat agtacaatca
      361 atatgcagac atgagcaatt taaaattgag tatagttttg aggggaaaca caaatttacg
      421 gttacaggaa ctaaatgaat caccgagata cctattgaaa aacttccttc cggaattgac
      481 tcctatgatg tctaatagga tgtatcaaac taccctatta tacttcggtg acaacccaat
      541 cgctttccga cagcattcct acatgttatc ccctatggtt ataaaaaaat aagggcctga
      601 tactgttgtc cattcaaaat ggtaataatg aaaataatac cgaatatttt tggttaattt
      661 tataaatcat cttggtaaaa cataccgatg gtttactcca aggaatgaca tccgggaaaa
      721 ttattcaatc aggtatgatt cccatttttt tccaaagaga cggatttact ttgcaatgaa
      781 tttatgaatc agatgaaaat aaaatggatt tacattattg cccttttgct gatggcaccc
      841 gggtatgcat ggagtcaaaa actcagttca ggtgaggtac catccattgt actcaacaat
      901 tttaaattag catatcccaa ggcttatgat gtcagttgga aaaaatcagg agaatcttat
      961 agtgccgagt tttgtgttga aaaaaaatgc aatttaaagg tctggttcaa caaatatgct
     1021 cagttgacca aggaaatgga agagctggta tttaataccg tcccagatag aatcaagaca
     1081 aaagtcaaag aaaattatgg caagtttaaa attgatgaaa tcacaaagga aactgtcggc
     1141 aagacaacaa aatacatcct gaaacttgaa tctaaatttg aagacttaaa aatcattttc
     1201 aatgaagatg gaaaagaatt atcaaaagaa gaggatgatt gattacttaa taccattacc
     1261 tattaaaata ttatatcata ttcagttata tatcataaaa aacattaatt tttaaaccaa
     1321 ataattaaat caacatgaaa aagattttgt ttttagcttt tatgatctca accatttcgc
     1381 tgattggctg tgatgacaag gagacattgc tcgatgcaga taaaataccg gcagaaatta
     1441 aaacatatgt cgaaaaacat ttccccggga aaaatattct acaatgtatc aaagatgttg
     1501 atgggttgga agtaagctat gatgtggttg ttgaaggagg ctataaactt gaatttaacc
     1561 gggacaaaaa gatcgacagc gtcaaaggtg tctctaaatt gcctgattcc gttgtacctg
     1621 agaaaatttt agcgtacgtg gcaagcaact acccgggcaa ttatatcatc gagtgggatg
     1681 ccgataagac gactcaggaa gtcaagctgg acaacaggct cgaactggta ttcaacagtg
     1741 ccggagactt tcttcgcatc gatgactaat caagaaaagt attacattga accggcaagt
     1801 tggcatttcg gcataattcg gtgattttat tgctcaaatc ctacatcggt ttcgcataac
     1861 gaatacaagt aggcgtgaat ttttcaattt caatctaata aaattccgga ttggcataag
     1921 ttgcttatgc tgaattcaaa cggaagaaac aggtcctccg gccgggtaaa atattcagga
     1981 tatcataaga caaaatccat aatgatgagc cctgtcagta ttttcctctt gcttcggcag
     2041 aattcaagta cctaagtcaa tccggattta ttttttcaaa tgaaaaaaca ggctccaggt
     2101 tggattattc ttttcctaat ttcccggtca cccattcctc caattccttg ccatgaagat
     2161 ttttggccag gatgacacca tttctgtcca ggatataatt ggccggaatt gttgtgatct
     2221 ttaacaagtc cagaaatgga gatgtatctc cctccatatg agaggcgtga ggccatcgat
     2281 ctgcaccatc ccgcttgatg gcattcaacc acactacccg ctcggattcc agtgcatagc
     2341 ctataatctg aaggcctgag tcatgaaagt tatcccagag tggaacaagt gtatttctgt
     2401 tttcttttct gcaaggtgca caccaggaag cccaaatatc gattatggtc aactgagacc
     2461 ctaatagcga acgcagagaa atggtgtctc catctgtcat tggcatcata aaatcaggaa
     2521 atgcatcccc cggagtcaag ggcaatccgc cggacttttt acacacctgt tgggtccaca
     2581 catgctccgg ttctattgct gacatgccct tgcaagtggc ttttaccaat tcgggaattc
     2641 tttcataatc acccttgatg cccgcccatt gcaaggctaa tgcaagtacg tacaaatcct
     2701 gactacccat caccgattcc accaattgtt gctgaaagtt gtatagagac ttttcatttt
     2761 ccaaaagatt ctcctcgttt acctcagcct gatcggaaag aaattgttta aaaagcctac
     2821 ttctattcgc tatcagtaca agaatgtccc tattgccttc caaagtactc gctatctgac
     2881 catttcgaag aaaagccccg gattcggacc gaatctctac cgggtaaccc ttcttcaata
     2941 aaaatggaat ataattcccg gagtccggat gattattctc cagtcggttg agatatttct
     3001 cgcctttttt ctggatggca agcaggaaaa tcccctcttt ttcaggcgct gaaaatttgt
     3061 cgaaccggaa atcacctgct tcatttatct cagcagaatc gacaacctta cattcatagg
     3121 aagacaccaa agaactgaat cccgtaggtt caagtaaata aacaaccggc ttgaaatcaa
     3181 catcactcag gacaatatgt ccggaaacac tattctgact gcatccggta aatgacagta
     3241 agataaggaa tgcaggaatc aaatacctgt agaaagcact ttgtgataat gattcagaat
     3301 aagccatctc atcaaatttt agccaacaat ctatgtcctt acgatggatt cggttagtat
     3361 attacatttt cccttcatcc acatctttaa taaatctgat cacgccgtcc ataaatacct
     3421 tctggtcatc ccacatggcc aggtggctgc cattggggca atacaggtat cgcccccttt
     3481 ggaccaattt gctttgctct tccattgccc cgggatccat ggtatcatac cgtgctccca
     3541 ccatcaaggt tggaacggta atttcatgca gccgcgactt gatatcccaa ccgtataatc
     3601 tggcttccgg cgggatgccc atttcactcg ggccctgcat caatacatat atatgattat
     3661 taccatgact gaaggaacga gtcacaggtt caggccattc tgggaggcgg caaaggtgca
     3721 ggttgtaata atgttcttgc aataattcct catatcgcgg attggtgtaa tcctttgaag
     3781 cctccagtgc tttgatttca gccagtgctc cgggatccat catgggagcc agggtattat
     3841 taaaatattg aatatattca ggaatgctgg ccatcatatt agacaccacc attcctttta
     3901 gatgatcctg atgcttgagt gcgtattcca tagccagaag tccgccccag gaattgccaa
     3961 tcagataaaa gttgttgctg tccgcaccaa tagcctgtct gacctgatcc acctcatcta
     4021 caaatcgttg aatagtccat aaactgtcat tctcaggttg atcgctgtag aaggagccca
     4081 actggtcata ttcatacagt tcgaagcctt gccgttgaaa gaaggtttcg aagcattcca
     4141 tatattcatg cgtcatcgcc ggcccaccat gcagcaagag aacctttaca tcaggattgg
     4201 tgccgaatcg cttggtccac accctaaaat caccataggg cgtatggatg ggaatcatct
     4261 tcacgcctgc cgattctata gaatcaccat aagcaaaata ctgctcaagc tgattgacac
     4321 ctgattgatt attgcaacat gacattaaaa acatcaacag gatgctcagg aaagggtata
     4381 tattaaatct tatcataata tttttagttg agcacagtta ttcaatggtt tctttttggt
     4441 tctgtcgccc taagtatccg gacagtttcg atacattctg agccatgact atggtggtcg
     4501 aaagcggcaa gaaatccctc aaaataaccg gagaagtgac acttcctttt aaaagacaag
     4561 atcagaataa tgaaagtaat acctgaaaat gcgccacctc cgaatgtgac tggacaaaga
     4621 gtaaacaaga ctccgatgac tcccggcaaa ttacacatat ttaccatcct ttccattcga
     4681 attgaagggt ctctgatcca aacatcaggc gatttttcgt gcaccaaaat ggttccggca
     4741 ccatggattg ttattacaag ttgtccaaaa ccttgctatc cacaagatta acttcggtga
     4801 tgaccatgtg aggatatagg tctctggcac gctgaattgt actcatagcg tgaggattcc
     4861 ttgcccagga acgccttgca atgccattgt acacgtccca atgtaacatc atggtcagac
     4921 gtcgttctgc ttcagggctt ccatccagca ccatgccaaa accgccgttg atgacttcgc
     4981 cccaacctac cccacctcca ttatgcagcg acacccaggt tgctccccga aaactgtccc
     5041 cgataacatt atgcactgcc atatctgccg taaatgatga tccgtcatag atattggagg
     5101 tctcccgata gggtgaatct gtccctgaca catcatggtg gtcacggcca agaaccacag
     5161 ggcctttcag cacaccggaa cgtatcgcat cattaaatgc gcgggcaatg gcaatcctgc
     5221 cttccgcatc agcgtaaaga atccgggatt tggagccaac gacaggaatc tgctctttgg
     5281 cattgcgaat ccattgtaga ttgtcctgca attgtggcat gatatccttt gccgatgtct
     5341 tcatcaacga agtaataaca tcacctgcga tttgatcgct catatcaagg tcttcctgca
     5401 gcccacttaa gcaaacccac ctaaacgggc cgaatccgaa gtcaaaacaa agaggaccca
     5461 tgatatcttc tacataggat gggtaccgat atccactttt cttgtcatga tcaaatacat
     5521 ctgcacctgc gacaccggct tccttcagga aggcattgcc ataatcccaa aaatacattc
     5581 ccctgtcatg gagggcattt atggcggcaa catgtcgaat caacgaagct gaaaccgcct
     5641 cacgaaattt tttatgatca cggagcatca aggccttggc ctcttcaaag gtatatccga
     5701 caggtgtata tcccaggtca tcaatattgt gcaacgaagt ctgatcagac cccagttcga
     5761 tatcgatgtt gtgaatcacg aaatactccc acagatcaac cacatttcca tggtaaacca
     5821 gagtaattac ctcctcggat gaccgtgccg actgcattct tgcaacacaa gcctccaaat
     5881 cggcatagat attttctttc agaatataac catcgtcagt ccgttggcgg atagcatcac
     5941 catctgtttc agccaacaca gttactgcat gggtaatgat gccggcaaga gcctgagcgc
     6001 ctgacatgcc tcccaggcct gaggtcacaa agacttttcc acgcagcgta tctccgagat
     6061 tcatcaacct tcctgcattg agcaaggtaa tagtcgtccc atggacaata ccctgcggac
     6121 caatatacat aaaggaaccg gccgtcatct gcccatattg agtcactccg agcgcgttat
     6181 acttattcca gtcattttta gtcgaatggt tagggatcat cataccattg gttacgacga
     6241 ctcttggcgc ttctttgtga ctggggaaca gacccatcgg gtggccggaa tacagggcca
     6301 gggtctgttc gtctgtcatt tcagacagat acttcatagt aagcaggtac tgggcccagt
     6361 tctggaagac tgctccattc ccgccataag taatcaactc atgaggatac ttggccacct
     6421 tataatccag attattctga atcataagca taatagcggc tgcctggggc gaaagtgccg
     6481 gatattcctc caccggccgg gccttcattt catactgagg cctgaagcga tacatgtaga
     6541 ttcgcccata agtatccaat tcatcggcaa attcaggtgc aagaaccgga tgccattccg
     6601 gttggaaata gcgcaaggca tttgctatgg caagttttcg ttcttccggg gtcaataccc
     6661 cgattaagtt tctcacgggg gcatgagcga ccaatggatc ccgaaggaca ggctccggca
     6721 atgtcgaagg aataccttgc cggattgccc gcgcaaattc tgaatcttgc atttccattt
     6781 attccaaatt tttatgttgc aagttaagca atttttaaaa agtcggaatt tagaataaga
     6841 tcagagtata atttaatagg gctattcaaa gaaaatattt atctttaata cttgaaaaac
     6901 ttttaattaa taaatgctta tgaaaaatac acttttattg acaactattc tgtcttttat
     6961 ccttattgta cctgccattg gccaaaaagt aaaatcggaa ggcaatcttg attttttgaa
     7021 gggtgtgaaa gaagtgacgc tcgcttataa gtacgataag atggctgtag gcaaatataa
     7081 agacggcaat gagtatgtcg caaagaaaac gaaggagttg aacgaaaagg aaaaagggaa
     7141 gggcgatgat tgggccaaaa aatgggtctc tgaccgctca gagcgttttg aacccagttt
     7201 tgaaaatctg ttgagtgaat attgcaaggg gatcgatttt gatcaaaaca acaagtcaga
     7261 atacaccatg actttagaga cggtgttcac tgagcccgga ttcaatgtcg gagtgatgcg
     7321 tcagaatgca tatatcaatg tcatcgtaca cttcaccgac aaggccggaa aagaattagc
     7381 caccttgagc attgataaaa ttccgggacg cgacgtgttc ggatacgatt tcgacaccgg
     7441 acaacgtata gaagaggctt attcaaaggc gggcaagacc attggtaaat acctgactaa
     7501 gaaggtagag taattgattc ttaccacgca aaagaccatc cggtcatcac ttcttgcgag
     7561 gtaatctgaa tggctatcgg aatttcactt tgctgattct gcaaaagtaa tttcggtagc
     7621 catttttttg gaatcacatt tttcctgaaa gtttcaaacg aagctcttaa cccgctcgct
     7681 tgtaaaaccc ttgttaacgg ttcgtgcagt gtttagctac tctttttcag tcaatacata
     7741 cacgctccct tttccttttc caatcattat cagcacctgt tcaactcgta agtattgcag
     7801 gtcttttttt aaggtgctca acctgatttc gggaaagtga tttgctaaat cacttacttt
     7861 attaggttga ttgctcatta taaaatcttt gattaacttt tgtctctcat tcagataacc
     7921 tcctttggat ttgaaaacat catatttttt ctccaatttt tcggttagtg ttttcaaact
     7981 ttccagaaaa aaaatcaacc acaagtctat ccgttcgttg tctgttcccc gacatttctg
     8041 tccgttcatc aaagcttcgt aataggcttt tttattttgc tcgatatggt tttcaaaaga
     8101 aatgtattga ataaacgaat aatcattttg gagcaaacaa agcgttgtaa gcaatctcga
     8161 aagtctgccg tttccgtctt gaaatgggtg tatgctcaaa aaatcataga taaaactgcc
     8221 aattacaatg agttgatgaa ttgttttttg tttcaaagtt tcgtttgtcc atccaactaa
     8281 ttccttcatt tctgatgcta ccaatgccgg atcggtagtg gcaaaaatcg tccgctgttc
     8341 gcctgctgga taagttgcca taactttgtt agaaaggaac ttgtaggttc ctctgtggcg
     8401 ctcatcttta ttgctgtatt tcagcaacat ttggtgtagt tgtttgatat agttttccga
     8461 aagtttgatc tcggaataat tgctgtaaat caattctaaa acttcgtgat agcctataac
     8521 ctcctgttca tcacgggttt ttagttctgt aattttaagg tctttcaata ggtcttttac
     8581 ttcttggtca gtaagcgttg ctccttcaat tctagtagat gagccgatac tttcaattgt
     8641 ggcaattttc cgcaattatt tcagataacg gttttcctgc ttttcggcaa tattccattt
     8701 acctttgaag ccgtcaatgt agccaatgag ttgtataatt tgctgattgg taacgaaatc
     8761 aaaattgagt ttctggatga acttatccat atactatctg tattttatcc aaaaaacaag
     8821 tgaaaaatgg gtattccgga gcaaagtgag ccactgattc cggaacaacg agagccacaa
     8881 tgaagcattc gataatttgg aacgtgcaaa gcaaaaggtg ttaaccccta tgaatggctc
     8941 gcagatgccc tcaataaaat cctatctcag cctgtcaacc ggatacatgg actgctccaa
     9001 ggatatatac cgaaaaatga agtgtagttg cccggtgggt tacaataata tatgtgaggt
     9061 ttttgtgtaa atagaagaaa tgtcactaaa ttaactttct gcggattgct tacgcctaac
     9121 cggcaaattg tatagggagc gccattttaa catcatggac gctaagtgta agcgcagtca
     9181 tggtcgtcca tggtccatga ttgaatagaa ttaaaatcat tcatttttgt atcctggatc
     9241 tccgggtttc gatcgaagcc gtcaacctgc tctcttgcca atttgtttgt tgggcgattg
     9301 tgcctaattc tcacggttta attttcacga ttttatcgcc tttcataacc acaagtacac
     9361 tatttttttg ccgtttatat tcaataaggt tcgtgtacat tttttccaaa ccttttagta
     9421 gtttgtcctt aaattctatc tgtttttctt tcttggtcat aagtaacttt ttaattcatt
     9481 atatttttcc gtgttaataa tttctacttt ttctcctaat gtttgttgag caattaattc
     9541 aggataacca tacgaattat caaatatgaa tgcgccgtca acaatgggta agtataaatt
     9601 gaaaaggttt tgtatgcctt tcaagtacct tctttcaata acgtcagggg gaatattatg
     9661 tcctccttct aagaccctgg ttatcactcg ttctttggcc agatcaacat ttttaagcca
     9721 gaaaaacaat aatgtcgtag tatatccttt tttcttagct gtttctattt tttgtctata
     9781 acttcgggtt gagagtgttg tttcaaaagc aaaggtttct ccgtttatca aaagttcatt
     9841 aatccgattt atcattattc gccctgcctc aaatgcaact ttttcaggtt gaaatggaga
     9901 aagacctttt gctatttcat cagcgtttac aaattcttta cattctatta tttctggcaa
     9961 aatcgtaaaa gaagctgttg tcttatcggc tccgttgcaa cctgcgatta tgtaaagatt
    10021 tttttctacc atattcgcaa atttactcat tttactaaaa ccaccttcgt tctgatacgc
    10081 ttttattcgt atatcgctca acatctttat atactctcca tgccgacatt ttccgtaaaa
    10141 aagtatcatt tcccacaagt tttcgttgga tattccggag caaagtaagc cactatgatg
    10201 tattcctttc ttggtaaatg caaagcaaaa ggaatcgatc cctatgaatg gctcgtagat
    10261 acccttaata aaatcccatt tcatcctatc aaccggatac atggattagt cccgggagat
    10321 atactgaaaa atgaagagta gttgccctgt gggttacctt ttgctaaaaa atctgtagta
    10381 cacaaatcag aacatccccc tccggaaact tccaatctgc gcaggatctc atctgcgtaa
    10441 gcgcaacccg ggctaaacag ctaagacctt acatttcaat aataaattat ac
//
LOCUS       DAOQMC010000063        10249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2532_length_10249_cov_5.223955, whole genome shotgun sequence.
ACCESSION   DAOQMC010000063 DAOQMC010000000
VERSION     DAOQMC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2532_length_10249_cov_5.223955"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1271)
                     /locus_tag="PKV31_03970"
     CDS             complement(57..1271)
                     /locus_tag="PKV31_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB92045.1"
                     /translation="MNISAKLAVRYIFGKKSSNVINIISAIAVFGIAVGTSALILVLS
                     VFNGFEDLISGLFQKFNPDIKITPAEGKTFEPDSSSVYKLQALNGINRVSATLEELAL
                     FDYHNSQTFGIVKGVDTNYAFVTAIRQSIVEGSYNLNGQDPPSVVIGAGVAQRLGVDI
                     EDKLELLRVYTPKRHNRSPMESPFTTRFINPAGIFAIQQEFDQEYMLSSLDFARELFQ
                     YEKEVSALEIRLDSTKNVKNIKTAISKIMGDNFVVKDRFQQDEAFMRLMNIEKWMSYA
                     IVCLTLLLVSFNMIGALWMIVLDKKRDIAILKSIGLGDRGVKAIFLRSGMLLSILGLL
                     IGFVIAVVLYTLQKTVGLVTIPEGFVVTTYPVSLRFIDFMVVGATVLLIGWLSSIPAA
                     RKASRIKTTFSD"
     gene            complement(1416..2720)
                     /locus_tag="PKV31_03975"
     CDS             complement(1416..2720)
                     /locus_tag="PKV31_03975"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNB92046.1"
                     /translation="MFTEVTTTIRSEQLMHYLWRTKQLERTNLRLTDGQRLEILDFGN
                     MNTNSGPDFLNGKIRIGNTILAGHIEMHINSSDWYAHGHQNDQAYKNVILHVVCRNDL
                     TVKTPNEVPIPVLLLMDYIDPAIVEKYARLHSDLIGIPCNRLHPEKVSPQVWNIWKER
                     LNIERLQNRVAWVEQEIEISAQDWEKIFYKQLFISMGTNVNKEAFALLADRLDFKIFG
                     RLSDSPDLLHAYVFGMSGMLDQDMDDPYFNCLKSEFKFLRTKYNLHSIPRVVWKYSRM
                     HPQNFPDIRIAQVAELIRANPHLFSTIMECSTTKQLKKLLSVKDLPVYWNFHYRLGGK
                     EGGRKKVMGDSMKNLIIINCIIPVMFIYGKYQNRSELRETALGLLDQLPPEDNYITRM
                     WQELGVPSHSALDSQALIQLKNHYCDRLQCLSCSIGHELLKK"
     gene            complement(2838..3524)
                     /gene="radC"
                     /locus_tag="PKV31_03980"
     CDS             complement(2838..3524)
                     /gene="radC"
                     /locus_tag="PKV31_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNB92047.1"
                     /translation="MKPLIIKEMNGEDQPRQKLQTLGSSSLSDAELLAIILRTGNRKL
                     SAIGLAQHVLHSCGGSISNLARKSIADLTAIEGIGPAKAIEIKAALELGKRKIAENNQ
                     LSEVISSSKDAYWAIAPQLMDLNHEEFWVILCNRAHKITDKVCISRGGMHGTVVDMKN
                     LMKIVVQSNAAAIVIAHNHPSGNRSPSREDRNLTTRIKEACHLMDVQLLDHIIVAGQQ
                     YSSFIDEGWL"
     gene            complement(3707..4861)
                     /locus_tag="PKV31_03985"
     CDS             complement(3707..4861)
                     /locus_tag="PKV31_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB92048.1"
                     /translation="MKNIYFALIFLSTVFANAQKTPAVELAVGDSLIKSYQFDRAREV
                     FYSVIREFPDNVDALYQLAYCHNRTGNYFDSRIYLKNATALDPSRADILILLAQTYDQ
                     LGFRDSSLTTYQRLLEIDTANGYYCRIVAEQMIPQKKFSEAINLLDRAIRLNDLDMES
                     YNLLAKVLYNLSLFDFAGQVANTGLQKNPRNPDLIFINMRIAYALKNYTEAAELGTRG
                     MSINSDSSRWNLFTGYAYYYLEDYKSCIPWLLSAVNDNSIGEQGLTLLANSYLNTGNQ
                     EKALEYIDIAIELAKDNNLESNLQNKAIILTREGKIREATKLMEQAYALNWNPKILFR
                     HASMQINNKHYASARSLLKKYLASGDAEFKKEATTVLKEIEQTMRTKQKK"
     gene            complement(5046..8441)
                     /locus_tag="PKV31_03990"
     CDS             complement(5046..8441)
                     /locus_tag="PKV31_03990"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB92049.1"
                     /translation="MRLLNHFTLTLAFCFSCINLFSQNIYLAYDGMSGAAGSPLHGGS
                     GGTGWADTWTVQNDNTDGYLYYGTGLTFAGLQQSDMAITGGESYLTSGRALDCNPAGP
                     FASYISENNGIGSNTGTTLWVSALVQKSQNNDQNISYTLHGSSIAWCDNCSQDNRVSF
                     GYFGNESNVGGERRWSLKIGNDVFTSTVPVIIGSTALLVARIDFNEGNTVINMYINPS
                     ELGYAGPPSMPSISQTTNMLLRVRSLSAYLGDVAGNGLMDEFRFASSYAVAAPDSGVD
                     ISLPPIADFTVTPASGQAPLSVSVDGSASFDPDGGSLSYSWNWGDGSPESSGVSSSHT
                     YPSNVYGQISVTLTVTAPSGLTGVLTKQITIFNSNGSFPCLSSITMINEASCLQDNGR
                     IRINAGQAGNAQYTFQSSAGITWTPTNGNEFHNLANGVYYATITGSNGCEDTYTLNMT
                     TDSTTCTGWSAYQCGLEMGMNVTGIADWNGEHPFLNRAKHIRDITTFHEGCNCWDSDV
                     ASQIIRDANHYPLYLPQPTTASPNTLVRFVLSTNNGNLRANEQYVFLYEGNGTFTLNG
                     AQTDQQNPGRVLFTVPATSGNIWIDVSFSDQANHMRNFKILKASEENVNLNEHTFNPV
                     FLSRLTPFSTIRFMDWGATNGSPLESWNQRPLPGDVTYSTSRGVPYEMMAALGNELGK
                     DIWVCVPHRADDDFVTQMAILFRDNFSPDRKIYLEYSNEVWNWIFEQAHYNNNTRPPN
                     LNYGRAYAEKAKRIFNIWTNVFAGQTHRLKRVLGIQGGFNYLNEEIMAQLSANEWDMG
                     SPTHYFGLNHSPSGNPPLSAGSTGEDVNLNARNNYFGSSGSPGFFNDVLQDYRNIKVF
                     GKTIVSYEGGQHYADFNDHPYQQAMYDAQYLQSMYTLYNDVLKSIRDHGNKLAMAFTL
                     SGVQESVYGSWGHLPDMYLQPPFTNSAPKYLACINNSCLPYKPETDFPLPVFQLISFD
                     VNVTGGEKVSIQWITGSSSDISEFVLERSTGDDAFLPITKLSPNKSQKIYRYEDKGLP
                     AGRYYYRLRSNNANGSFLYSQIKSAEIHPENELFISPNPGFDKIYLLYKKKSHASPAT
                     LFDANGKILQSFRQIPEYIDLSNEPSGMYFLHYREKTYKIVKR"
     gene            complement(8628..9716)
                     /locus_tag="PKV31_03995"
     CDS             complement(8628..9716)
                     /locus_tag="PKV31_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB92050.1"
                     /translation="MGFLFIDMKPADYTSLNLNGTSLEGPEILEFCNAQSEEHYTQLS
                     QFLSQWLDDREDLEVKTSGSTGTPKAICIPKARMLQSAAATARFFGFQPGETALLSLP
                     VSHIAGKMMVVRALYSHLNLKYIRPSHHPITELGTQDKINFAPFTPMQLSEANDTGNI
                     DTILLGGGPVSPDLELKLQMWQAAIYHGFGMTETLSHIALRRVNGPDASGVYKALPGV
                     ELTVDERSCLVIRAPFVEGPVVTNDIVDLQGNDAFVWRGRIDHVINSGGIKLFPEEIE
                     KKLNTCIDRDYFIAGIPDPIFGEKICLFIEGEAWSYEQQDKLSNIMDQCLTKYERPKK
                     LYFVLNFSRTLSGKIQRKTTIEYVLQNR"
     gene            complement(9692..>10249)
                     /locus_tag="PKV31_04000"
     CDS             complement(9692..>10249)
                     /locus_tag="PKV31_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNB92051.1"
                     /translation="IRYIRREFSADEVEIRLDANGAFTPTEAKEKLDRLAKYDIHYME
                     QPIRAGQWQETARLVEDSPIKIALDEELIGINTANRQRELIETIRPAILILKPALLGG
                     FKACDDWTALLEDIGGYRIITSALESNIGLNAIAQYAALTASPHAYGLGTGMLYSNNF
                     PSPYHVDAQGLHYRRDQTWDFSSLT"
BASE COUNT         2707 a         2186 c         2290 g         3066 t
ORIGIN      
        1 ccttaatctg atgtcgggct ttcaaccagt gaaagtccag ttcccaatct tccattttaa
       61 tcactgaatg ttgttttgat gcgggaggct tttcttgccg ccggtatgct tgaaagccag
      121 cctattaaga gcacagtcgc ccccacaacc atgaaatcaa taaaacgcag ggaaacggga
      181 taagttgtga ccacaaaacc ttccggtatg gtgaccagac ccacagtctt ttgaagagta
      241 taaagcacaa ctgcgattac aaaaccaatc aacaagccga gtatagaaag aagcattccg
      301 ctcctcagga atattgcctt tactcctctg tcgcctaatc cgattgactt aagtatggcg
      361 atgtcccgtt ttttgtccag tacaatcatc cacagcgcac caatcatgtt gaatgatacc
      421 agcaggagcg ttaggcagac tatggcatat gacatccatt tttcgatatt cattagtcgc
      481 atgaaggctt catcctgctg aaaccgatct ttgacaacaa aattatcgcc cattatcttg
      541 cttatagcgg tctttatatt ttttacattt tttgttgaat ccaatctgat ttccagcgct
      601 gaaacctctt tttcatactg gaataattca cgggcaaaat ccagagacga aagcatatat
      661 tcctggtcaa actcctgttg tatggcgaaa atcccggccg gattaataaa tcgtgttgta
      721 aatggtgatt ccatgggtga gcggttgtgc ctcttcgggg tatacaccct gagcaattcg
      781 agcttgtcct cgatatccac gccaagtcgt tgtgctacgc cggctccaat aaccacggat
      841 gggggatcct gaccgttcag gttgtaagat ccttcaacga tgctttggcg aatagcagtg
      901 acaaaagcat aatttgtatc tacccctttt actataccga aggtctggct gttgtgatag
      961 tcaaacaagg ctaactcctc aagtgtcgca cttaccctgt tgataccatt cagagcttgt
     1021 agcttgtaga cgctgctgct gtccggttcg aaggttttgc cctcagcagg agttatcttg
     1081 atatccggat tgaatttttg gaacaatcca gaaatcagat cctcaaatcc attgaatacg
     1141 gacaagacaa ggataagggc agaagtaccc acagcgatgc cgaatacagc aattgctgaa
     1201 atgatattga tgacattgga ggattttttt ccaaagatgt atctgacggc cagttttgca
     1261 gaaatgttca atacccggta tttgctccaa agataaaatg attggacggt tttcaaaggg
     1321 agaaattatt tcaaagtgat agggatctct ctgagagggc cttatcaaaa gccatcttat
     1381 ggtacaatca tctatgatga gtgagggctc tgatactatt tttttaatag ttcgtgaccg
     1441 atgctgcaac tcagacactg caatcggtca caataatgat ttttcagctg tatcaaagcc
     1501 tgtgagtcaa gagccgagtg acttggcacg ccaagttcct gccacattcg ggtgatatag
     1561 ttatcctcgg ggggcaattg gtccagcagt cctaaagccg tttcccggag ctcacttcta
     1621 ttctgatatt tcccataaat aaacattacg gggatgatac aattgataat aatcagattt
     1681 ttcatcgagt cgcccatgac ttttttccgg cctccttcct tgccgcccaa cctgtaatgg
     1741 aaattccaat acaccggtaa atctttgacg gaaagcaatt ttttcaattg ttttgtagtc
     1801 gaacattcca ttatggttga aaagagatgt ggattagccc tgattaattc agcgacttgt
     1861 gctattctga tatccgggaa attctgtggg tgcattcggg aatatttcca tacaactctg
     1921 gggatagaat gtagattgta tttggttcgc agaaatttga attccgattt caggcaatta
     1981 aaataaggat catccatatc ttgatccaac atgccggaca tgccaaatac ataggcatga
     2041 agcaggtccg gagaatcgga caatcttcca aatattttga aatccaatcg gtctgccagc
     2101 aaagcaaagg cttccttatt gacattggtg cccatactta taaacaattg cttataaaaa
     2161 atcttttccc aatcctgtgc tgatatttca atttcctgct ctacccaggc aacgcggttt
     2221 tggagccttt ctatattgag tcgttccttc cagatgttcc atacctgtgg actgactttt
     2281 tccggatgaa gtcggttgca cggaattcca ataagatccg agtgcaggcg agcgtatttt
     2341 tcaacaatag ccgggtcaat atagtccatg agcagcagta ccggaatcgg tacttcatta
     2401 ggagtcttta cggtcaggtc attgcggcag accacatgaa ggattacgtt tttgtaagcc
     2461 tgatcgttct gatggccatg ggcataccag tccgatgagt tgatgtgcat ttcgatatga
     2521 ccggccagga tagtattgcc tattcgtatc tttccattga gaaagtcagg gccactgttg
     2581 gtattcatat tcccaaagtc cagaatttct agcctttggc catcggtaag cctcagattg
     2641 gttcgctcca attgtttggt tcgccacaaa taatgcatca attgctcgga gcgaattgtg
     2701 gtggtaactt cagtgaacat aatgagaaat ttataaaatt aaaaaatgat aattgattgg
     2761 tgatatatgc atggatcgaa cagaaaagat gcttcaaacc caatattaca aaccggcaat
     2821 agactggaat ctcctcttca aagccacccc tcatcgatga aactagagta ctgctgtccg
     2881 gcaacgatga tatggtctag cagctgaaca tccataagat ggcatgcctc cttaatcctg
     2941 gtggtcaggt tcctgtcttc ccgactgggg cttctattgc cactcgggtg gttgtgggcg
     3001 atgacgatag cggcagcatt ggattggact acaatcttca tgagattttt catgtcaaca
     3061 acggttccat gcattcctcc ccttgatata cacaccttat cagttatttt atgtgccctg
     3121 ttgcacagta ttacccaaaa ttcctcgtga ttgagatcca ttagttgcgg agctatggcc
     3181 cagtaggcat cttttgaaga tgaaatgact tcagaaagtt ggttgttttc tgctattttt
     3241 cgtttaccga gctccagggc ggctttgatt tctatggctt ttgctggccc gataccttcg
     3301 atggcggtta ggtcggcaat gcttttgcgg gccagattgc tgatgcttcc accacaacta
     3361 tggaggacgt gttgtgccag gccgatggca gaaagtttcc gattgcctgt cctgagaatg
     3421 atggcaagta gttcagcgtc acttaatgaa gatgagccga gtgtttgaag tttttggcgg
     3481 ggttgatctt ccccattcat ttctttgatg atgagtggtt tcatatggat gattttgtgc
     3541 acgctaagtt aatactctct ctcggaatgg aaaatatatt tgtggattaa tttttcagaa
     3601 aggataatat cagccggaga tatcgttaaa ttaaaagtac taaaagagca atgttaaaga
     3661 cctgttgtaa ttcggatacc gtaacttcag attaatcggg cactctttat tttttttgtt
     3721 ttgtacgcat agtttgttct atttctttca aaacagttgt ggcttctttt ttgaattccg
     3781 catcaccact tgctaaatat ttttttaaca aagagcgtgc agaggcataa tgcttgttgt
     3841 tgatttgcat ggaagcatga cgaaacaata tcttaggatt ccaattcagg gcgtatgctt
     3901 gttccatgag ctttgttgct tcacgaattt ttccttccct ggttaagatg attgccttgt
     3961 tctggagatt gctttccaga ttgttgtcct ttgccagctc gattgcgatg tcgatgtatt
     4021 ccagggcttt ttcctgattt ccggtattta ggtaggagtt ggccaataat gtcaggcctt
     4081 gctcgccaat ggaattgtca ttgactgcac ttaacaacca gggaatacat gacttatagt
     4141 cttcgagata ataataggca tatcctgtaa atagattcca ccggcttgaa tcactgttga
     4201 tactcatacc tcgggtacct agttctgccg cttctgtgta gttcttcaga gcataagcaa
     4261 tacgcatatt gataaagatt aagtcaggat tgcgtggatt tttctgtagc ccggtattag
     4321 ccacctgtcc ggcaaaatca aacaaggata agttatacaa tactttagca agaagattat
     4381 aactttccat gtcaaggtca ttgagccgga ttgcccgatc aagtaaattg atagcttccg
     4441 agaatttctt ttgtggaatc atttgttctg caacaattcg gcaataataa ccgttggctg
     4501 tatcgatttc aagtaggcgt tgatatgttg ttagtgagct gtctctgaag cccaactggt
     4561 cataggtttg agcgagaaga atgagaatat ccgctcttga ggggtctaat gccgtcgcat
     4621 tttttaaata aattctgcta tcaaaataat tgcctgtccg gttgtggcaa taggccagtt
     4681 ggtacaaggc atccacattg tcggggaatt ccctgattac cgaatagaaa acctcacgcg
     4741 cccgatcgaa ctggtacgat ttgatcaatg aatcgcctac agctaattca actgccggtg
     4801 ttttttgtgc gttggcgaaa accgtggaaa gaaaaatcag agcaaagtat atatttttca
     4861 tagcgaacaa atatataact aaatattaag ttatattaga atctttacat acaatttgcc
     4921 tttattccgc aactttatta gttcctgatg caattatgca gacaggatct cggtatctct
     4981 tgcatcatgc aaagggacaa tcggttggaa agtccggttg atttactatg tggataggcg
     5041 taagtttagc gttttactat tttataagtc ttttcacgat agtgcaaaaa atacattcct
     5101 gaaggttcgt tactcaagtc gatgtattcc ggtatttgtc tgaaagattg caaaattttc
     5161 ccattagcat caaacagtgt ggccggagat gcgtgacttt tctttttata tagcagataa
     5221 atcttatcaa aacccggatt gggtgagata aacagttcat tttccggatg gatttcggcg
     5281 gatttaatct gcgaataaag gaaactacca ttggcattgt ttgacctcag ccgatagtaa
     5341 taccgccctg ccggaaggcc tttatcttca tacctgtata ttttttgcga tttgttgggc
     5401 gataatttgg ttattggcag gaatgcatca tccccggtac tacgttccaa tacgaattcc
     5461 gaaatatctg aagatgagcc tgttatccat tgaattgaca ccttctctcc gcctgtaacg
     5521 tttacatcaa agctgattag ctgaaatacc ggtaacggga agtctgtctc tggtttataa
     5581 ggaagacatg aattgtttat acaggccaga tatttaggcg cggagttggt aaatggtggt
     5641 tgcaagtaca tatccggcag gtgtccccat gagccataaa cactttcctg tactccggat
     5701 agtgtaaatg ccatggctag tttgttgccg tgatctctta tggacttcaa cacatcattg
     5761 tataaagtat acatgctttg gagatattgt gcatcgtaca tggcttgttg ataggggtga
     5821 tcattgaagt ctgcgtagtg ttgtccccct tcatagctga ctatggtttt accgaatact
     5881 ttgatattgc gatagtcctg gagaacatca ttaaagaatc ccggagaccc tgatgaaccg
     5941 aaataattgt tccttgcatt aaggttgaca tcttcgcctg tcgagccggc cgataatggc
     6001 ggattgccgc tcgggctgtg attcaatccg aaatagtggg ttggtgaccc catatcccat
     6061 tcatttgcgg ataattgggc cattatttct tcattcaggt agttgaatcc tccttgtata
     6121 cccaatacgc gcttcagccg atgtgtctga cctgcaaata cattggtcca gatattgaat
     6181 attcgttttg ctttttctgc gtaggctcta ccatagttta ggttgggggg gcgtgtgttg
     6241 ttgttgtagt gcgcttgctc gaatatccag ttccagacct cattgctgta ttcaagataa
     6301 atttttcggt caggactgaa attgtcgcgg aaaagaattg ccatttgagt tacaaagtca
     6361 tcatcagccc gatgaggtac gcatacccaa atatccttcc ccagttcatt gcccagcgct
     6421 gccatcattt catagggcac gccccttgaa gttgaataag tcacatcgcc cggcaggggt
     6481 ctttgattcc aactctccaa tggacttcca ttagtggctc cccaatccat aaaccggatg
     6541 gtcgagaagg gcgtcaatcg gcttaaaaaa acagggttga aagtatgttc attaaggttg
     6601 acattttctt cagacgcttt taaaatttta aaattgcgca tgtggttggc ctgatcggag
     6661 aaggatacat caatccagat attaccggaa gtggcaggta cggtaaataa taccctgccc
     6721 gggttttgct ggtctgtctg agctccattg agcgtaaatg tgccgtttcc ttcatacagg
     6781 aagacatatt gttcattggc gcgcagattg ccattgttgg ttgacaatac aaatcttacc
     6841 aaggtattgg gagaggctgt cgtcggttga ggtaagtata aaggatagtg gttggcatcc
     6901 cggataatct gtgatgctac atcactatcc caacaattac atccttcgtg aaaggtggtg
     6961 atgtcgcgga tatgtttggc gcggttcaag aagggatgtt caccattcca gtccgctatt
     7021 cctgtgacat tcatccccat ttcaagacca cattgataag cgctccagcc ggtgcaggtt
     7081 gttgagtcgg ttgtcatgtt caatgtatag gtatcttcac aaccatttga tccggtgatg
     7141 gtggcgtaat ataccccatt tgcgagattg tgaaattcat tgccattcgt tggtgtccac
     7201 gttatgcctg cgctactctg gaaagtgtat tgagcgttgc ctgcctgtcc tgcattgatc
     7261 cttattcttc cattgtcttg caggcaggag gcttcattga tcatggtgat tgagcttaag
     7321 cagggaaaag aaccattgct attaaaaatg gttatttgtt tggtcaatac gccggtaagc
     7381 cctgaaggag ctgtgactgt aagagtcacc gatatctgac catatacatt ggaagggtat
     7441 gtatgggagg atgaaacacc ggatgattcc gggcttccat cgccccaatt ccaggaatag
     7501 ctcaggcttc ctccgtccgg atcaaaggat gcgctgccat cgactgaaac gctgagtggc
     7561 gcctggccgg atgccggggt cacggtaaaa tctgcaatag ggggtagaga aatatcgact
     7621 ccactgtccg gagccgccac ggcgtaggaa gatgcgaatc tgaattcatc catcaggcca
     7681 ttcccggcaa catcgcccag ataagcggaa agtgaacgaa ccctgagcag catgttggtt
     7741 gtttgggaaa tggaaggcat ggatggaggt ccggcataac ccagttctga agggttgata
     7801 tacatattga tgaccgtgtt gccttcatta aagtcaatac gtgcaacaag taaggctgtt
     7861 gagccaatga ttacagggac tgtagaagta aacacatcat tacctatctt caacgaccat
     7921 cttctttcac cacctacatt ggattcgttg ccaaaatatc caaaactaac tctattgtct
     7981 tgactacaat tgtcgcacca cgctatggaa gacccatgca gcgtatagga aatattttgg
     8041 tcattattct gacttttttg gaccaaggca cttacccaaa gtgtcgtacc ggtgttgctg
     8101 ccgattccgt tattttctga aatataagag gcaaaaggac ctgcggggtt gcaatctagg
     8161 gctctcccac tagtcagata actttcacca cccgtaattg ccatatcgga ttgttggagt
     8221 ccggcaaaag tgagtccggt tccataataa aggtagccgt cggtattgtc gttctggaca
     8281 gtccaggtat ctgcccatcc ggtcccaccg gagccaccat gcagcggaga tcctgcagcg
     8341 ccagacatgc catcataggc caggtagatg ttttgagaaa atagattaat gcaggaaaag
     8401 cagaatgcca gagtcaaggt aaaatgattt aaaaggcgca ttgtgttaaa gatttaggta
     8461 gtaacaatga ttattcacgg atgatgagca aaaaggatcg attaatccgg gtcaatatta
     8521 agtatattta ctaaaatatg gaaattttca aattactttt aatcaaatta aaaattttaa
     8581 catcaaactg aacttccggt aaacaaagcc tcggtgtaat acaataatta tcggttttgt
     8641 aaaacatatt caattgttgt cttcctctga attttaccgg ataatgtcct ggaaaaatta
     8701 agtacgaaat ataacttctt gggacgttca tattttgtca gacattgatc cattatattg
     8761 gacaatttgt cttgttgttc gtaactccag gcctctcctt caatgaagag gcaaattttt
     8821 tctccgaaaa taggatccgg aatgccggca atgaaataat ctctatcgat gcaggtattt
     8881 aattttttct cgatctcctc cggaaacaac ttgattcctc cactattgat gacatggtca
     8941 atcctacccc gccatacaaa ggcatcatta ccctgtaaat cgactatatc attggtcacg
     9001 acggggccct ccacgaatgg tgccctgatg accagacaac ttctttcgtc cacggttaat
     9061 tccactccag gcaatgcttt atacacgccg gaagcatccg gcccgttaac ccttcttagg
     9121 gcgatatggc tcaatgtttc tgtcatgccg aatccgtggt atatcgccgc ttgccacatc
     9181 tgaagcttta gttccaggtc cggactgaca ggcccgccac ccaataggat ggtatcgata
     9241 ttccctgtat cattggcttc gctgagttgc ataggtgtaa atggtgcaaa attgatcttg
     9301 tcctgagtgc ccaattctgt aatcggatga tgagaggggc gaatatattt tagattcaga
     9361 tggctgtata gcgctctaac caccatcatt ttgccggcta tatggctgac tggcagactc
     9421 aacaaggctg tctctcccgg ttgaaaaccg aaaaaacgag ctgttgcagc agcactttga
     9481 agcattctcg cttttgggat acagattgct ttaggagttc cggtggagcc acttgttttg
     9541 acttccaaat cctccctatc atccagccat tgggataaga attggctaag ttgggtgtag
     9601 tgttcctcac tttgtgcatt acaaaattcg aggatttccg ggccttcgag tgaggttccg
     9661 ttcaagttga gagaggtata atcagcaggc ttcatgtcaa tgaagagaaa tcccatgttt
     9721 ggtcacgacg atagtggagc ccttgtgcat cgacatgata gggtgacgga aaattattgg
     9781 aatagagcat gcccgtgcct aatccataag catgtggcga tgccgtcaac gccgcatact
     9841 gagcgattgc gttgaggcct atattacttt ccaatgcaga ggtgatgatg cgataacctc
     9901 ctatatcttc caacagtgct gtccagtcat cacatgcctt aaagccgcca agaagtgcag
     9961 gcttaagaat caggattgcg ggtcggatgg tctctatcaa ttctctttgt cgattggctg
    10021 tattgatgcc gattagctct tcatccagcg caatcttaat cggggaatcc tccaccaggc
    10081 gtgctgtctc ctgccattgg cctgcccgga ttggttgttc catataatgg atatcatatt
    10141 tggccagtct gtctagtttc tctttagctt ctgtaggagt aaaggcccca ttggcatcga
    10201 gacgtatttc cacttcatct gccgaaaatt cgcgccggat atacctgat
//
LOCUS       DAOQMC010000064        10126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2585_length_10126_cov_5.534704, whole genome shotgun sequence.
ACCESSION   DAOQMC010000064 DAOQMC010000000
VERSION     DAOQMC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_10126_cov_5.534704"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1134)
                     /locus_tag="PKV31_04005"
     CDS             complement(181..1134)
                     /locus_tag="PKV31_04005"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HNB92052.1"
                     /translation="MLIAVAVLSTVIMSGCKTATYTHVDQVIAPRFSIPVDMRSVNLL
                     DRSVSPYAASKTTLPTPTDKARTTMLNSVLSGLPVSSKRILQPLKEGRENGPATEIKK
                     TGIREVASGAEGLLSLEQYEFSETRTYKDITKETRDPSGRKVTIPAVQGTRQVYLRTY
                     WRLYDVRSGSVIYKIPQFSENIYETEGLTRAAVNAQMDTTNIVTVATLSQKLSSELIR
                     DINPEHIKSYWTYYIKGHDIISRSGRMIEMEDFAGAVSFLSQNIRVIESEKLRLRASY
                     NLIVAYYFNGQKDKALQLAGHEYNKTGKYEFKSLYDKIYSR"
     gene            1820..2449
                     /locus_tag="PKV31_04010"
     CDS             1820..2449
                     /locus_tag="PKV31_04010"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB92053.1"
                     /translation="MKKFFFLFLTAGFLLTSCTSNPEGDKAETTEAQQAPTEAVGTAY
                     TVNTQESTLRWEGKKVSATHHGVVNIKSGKLMMDGDNLAGGEFAVDMNTIVNEDLQGE
                     WNQKLVNHIKSEDFFHVQQHPEAIFQITEVKATDNPNQVVVSGNLTIKGIAKNITFNA
                     DVVEKSADKIQMKADFNIEREQWGITYTGMADDLISKEINFKVNIVANS"
     gene            3566..5002
                     /locus_tag="PKV31_04015"
     CDS             3566..5002
                     /locus_tag="PKV31_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB92054.1"
                     /translation="MNLEEITARLNQLRELSGQLDVSESQRKGMIGLVAEYTNQFING
                     LSTVSGFSEKQPGSLAIGNGGIPLEQLLSSYQSEVVETGINAASGTHLGYIPGGGIFA
                     AALADFIAAVTNPYAGVYYASPGAVTIENEVIHWLKSIFSFPADAVGNLTSGGSVSML
                     IAFNAARDKYGVKNEKVRQSVVYLSQQVHHSAQKALRILGLEDVIIRYVPLDDRHRMN
                     ADILALLVAEDKSRGLHPFLVVATAGTTDTGAVDPLDAIADITEMHNLWFHVDGAYGG
                     FFILTSKKELFRGIERANSIIVDPHKSLFLPYGLGAVLIRDKDAVLHSNRYAANYMQD
                     ALGDELVKSPSNLSPELTKHFRGLRMWLPLQLHGLAPFIACLEEKLLLTAYFRAKMVA
                     VGFKVGPEPDLSVSYFYYPFESDADKKNSLLMKEIHADGDVFLSSSVIDGRYVIRMAI
                     LAFRTKRETIDRAITMILRCLDKVRHVG"
     gene            complement(5007..6611)
                     /gene="nadB"
                     /locus_tag="PKV31_04020"
     CDS             complement(5007..6611)
                     /gene="nadB"
                     /locus_tag="PKV31_04020"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNB92055.1"
                     /translation="MAQTSDLVIVGSGIAGLSLAIQVAQRRKDLKITVLAKTTEKESN
                     TNFAQGGIATVWDVEVDNFEKHIADTLDAGDGLCDKEVVEIVVKSAPERVRELIDWGA
                     NFDKTQTGEYDLGREGGHTENRILHYKDITGREIQRTVVEKAAQFDNIEILEHYFAID
                     IITQHHLGYNVSRLTPNIECYGVYALDRKTNEIKTFLARQTVLASGGSGQVYQTTTNP
                     LVATGDGMAMFYRAKGRVCNMEFVQFHPTALYNPGGDKPDFLISEAVRGFGAILKDAQ
                     GREFMQKYDERLSLAPRDIVARAIDNEMKILGADKMFLDCRHLDKDGFLRHFPNIYEK
                     CKSIGIDPFVQMIPIVPACHYICGGIKIDHEGRSSINRLYAAGECTNSGLHGANRLAS
                     NSLLEGLVFGYRAAMDILATIDKYVICKGIPDWNARGTSDPKEMVLITQSRKEVNEIM
                     SNYVGIVRSNVRLKRADDRMYLLYKETEELYNTTTISPQLCELRNLITISYLIIKSAT
                     MRHESRGLHYTTDYPDKLPFLQDTLM"
     gene            complement(7079..8512)
                     /gene="pyk"
                     /locus_tag="PKV31_04025"
     CDS             complement(7079..8512)
                     /gene="pyk"
                     /locus_tag="PKV31_04025"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNB92056.1"
                     /translation="MADRSFQNTKIVATLGPASDSYETMVELVKAGVDVFRINMSHGS
                     HEVAARQIETISKLNKDYKINVGILADLQGPKLRVGQIENNVMELNAGDILTFVNEKC
                     VGNAEKIYMSYKNFAADVEVGEKVLIDDGKVGLEVIETNNVDTVRLKVLYGKELSSNK
                     GVNLPDTLVSLPAMTEKDLNDLEFLMTQPVNWIALSFVRGPKEVKALKDKIKQHKHHA
                     LVIAKIEKPQAVEKIEKIVKATDGIMIARGDLGVEMPIEKLPGIQKRIIALCVQFGKP
                     VIVATQMMDSMITNPSPTRAEVTDVANAVLEGTDAVMLSGETSVGAYPIETVLAMNRI
                     IAEAEKNYPKNHIRPYPSRKSETFMSDVVCFNAAKTAEELGASAIIGMTAMGYTGFKI
                     SSYRSPAEIFIFSDRKEIAGMLNLVWGVRCFHYDGFNSTDETITEVLQILKSKGRVRS
                     GETVVFVGSMPLKSKGMTNMMKVALVE"
     gene            complement(8852..9538)
                     /locus_tag="PKV31_04030"
     CDS             complement(8852..9538)
                     /locus_tag="PKV31_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92057.1"
                     /translation="MVNKTVIVSGASGNLGTAVVNFYLDRGDQVIGLVHRSGGQPRNI
                     ANYVECAVNLLDELAVEECVKQLLEKFGKIDIAVMTAGGFAMGDLSDTGISELEHQYR
                     LNFETAYNLTKPLYEHMKLNHGGRLFFVGSGQGLDTSKGKHVVAYSLSKSLLFQLSNI
                     INADTAHTGIRAHVIVPSIIDTPRNRESMPDADFSKWEKPADIAAIIAKYADNHQSDK
                     QTIVVKDELK"
BASE COUNT         2699 a         2277 c         2160 g         2990 t
ORIGIN      
        1 gagctaatcc ttggttatat acagacaaat gacgtgtagt tgctcggtcg gttaccattg
       61 gatgacctgc cgatacgcat caatattccg ttttgatgaa tggtgcccca aaagtgaatc
      121 atcaaaatac ttcctcatgc cctttagctt tgttgaatat tgacgatttg gctccggaat
      181 ttatctggaa taaatcttgt cataaagact tttgaattcg tactttccgg tcttgttgta
      241 ctcatggccg gccaattgca gagccttgtc cttctggcca ttgaagtagt aggcaacgat
      301 caggttgtag ctggcccgca gccttaattt ctcactttca atcacgcgaa tattctgact
      361 cagaaagctt accgctccgg caaaatcttc catttctatc attcttcccg aacgactgat
      421 gatgtcatgg cccttgatgt aataggtcca gtatgacttg atgtgttccg gattaatatc
      481 ccggataagt tcactgctga gcttttgaga caaggtagcc accgtgacta tgttggttgt
      541 atccatttgt gcattaactg cagccctggt caatccttct gtctcatata tgttttcact
      601 aaattgagga atcttataga ttaccgaacc ggatcttaca tcatacaatc gccaataggt
      661 gcgtaggtac acctggcgtg tgccctgaac tgcaggaatg gtgacttttc tgccggatgg
      721 atcgcgggtt tccttcgtga tatctttata ggtacgggtt tccgaaaact catattgttc
      781 caacgatagc aacccttctg ctccggaagc gacttccctg atgccggtct ttttgatttc
      841 agtagccggg ccgttttccc gtccttcttt caacggctgt aaaattcgtt ttgatgaaac
      901 aggaagtccg cttaatacgc tgttgagcat ggtggtgcgt gccttgtctg tcggtgtagg
      961 aagggttgtt ttactcgctg catatgggct caccgatcta tcaagcaagt tgacagatcg
     1021 catgtcgaca gggatggaaa accggggtgc aataacctga tccacatgag tatatgtggc
     1081 agtcttacat ccggacatta ttacggtgga caaaacagca acagctatta acattcgttt
     1141 taccggaaaa aattgcatgt aagagttatg tttattgatt aaatattgac ttcggcctaa
     1201 aaatacaaaa aaggatgaat cctcatctct ttggggcgtt gtccaaccaa caaatatcct
     1261 ttcgattaat attgatggat aaaaaaacta ttgtcccgga ttttgttttc gccattgtgt
     1321 ggccgggtat tttcttcatc cgggggacat agagctgtgt cgctggcatg tcactttctc
     1381 ttcagtataa cagcatcaaa gctcctcggt ttttatcatt tactttcggg agaaatcatt
     1441 tatttatcta aatacaaata ttatctgact cattgattga ggactgtcgt ctcaactaca
     1501 atatactcta catcagctaa atcattgact ccatcactct ttccctcgtc attgggggtg
     1561 taggcattgg aaaacatgaa ggctgttgtg gtgcaagcga tggatataat gtcttccccg
     1621 ccacaaactc cttgccctca atgtatgaat ctgtttactt tcatcgtttt caatgcaact
     1681 gacaattgcc ggagcggtct ctattcaggc aaaaaaaaac aacttgccat gtattttctt
     1741 aatgtagctt aactgccgcc tattacaata tccatacctt tgctcccgtt attaatttat
     1801 aaattatcat aattaaaaca tgaaaaaatt ctttttttta tttctgaccg caggtttttt
     1861 actgacctct tgtactagta atcctgaagg cgacaaagct gaaactaccg aagcacaaca
     1921 agcacctact gaagctgtcg gaactgctta taccgtgaac acccaggaat caacccttcg
     1981 ttgggaagga aagaaagttt ctgccacaca ccacggtgtc gtcaacatta aatcaggcaa
     2041 gttgatgatg gatggagata atctggccgg tggtgaattt gccgtggata tgaacaccat
     2101 tgtaaatgag gatctacaag gcgaatggaa ccaaaaattg gttaaccaca taaagtctga
     2161 ggatttcttt cacgtgcaac agcatccgga agctattttc cagattacag aggtaaaggc
     2221 aaccgacaat cccaatcaag tggttgtatc cggaaacctg accatcaagg gcattgctaa
     2281 aaacatcaca ttcaacgccg acgtagtcga aaaatctgcc gataaaatcc agatgaaggc
     2341 cgacttcaat atcgagcgtg agcagtgggg tattacttac accggcatgg ccgacgacct
     2401 gatctctaaa gaaattaact tcaaggtcaa tatcgtagcc aacagctaaa ataacccaaa
     2461 aaagacatga agaagggagg cataggcttc ccttttttgt ttggagatga ttgatttttg
     2521 aggcaaatcg aaactgattt acgtggaata ttctgctatg aaacaataat ccggtacggt
     2581 gaagtagcca caggaaaaat tgaagtgaat tcagtatcaa agacatgttt caatctcaca
     2641 ttatcattga gtattgaaat gcagatagaa gaaataaaaa tagttgtgac attgggtagg
     2701 ccttctatta tgtctctttc atcacatttt gcaaaaactt atcaacacga tacatggcag
     2761 ggaaaatctc cggattaaaa ggatagatgt tacttaatca accatctgct acggggtcat
     2821 tactaaatca actatctgcc aaaaccaaat aactttcttc tgaaaaccac agctatgcct
     2881 ttgccttcca ggacgattac ctgaataaag tgtgtatatg ggagactttt gttattatgc
     2941 aagcatcggt aacaaatcat tgcctgcctt tgtagttcgt ggaaattatc ttggtgcacc
     3001 cttatatgat aaatgtcgct gctcctgaga atcaaaacaa cgaattgaag ggatccattg
     3061 ggcattgata gttgtaaatg ttaactattc cgacaaccaa aacgaaattc cattatggct
     3121 tttggtatgt taaatgcatc atcggggaaa taaataactg cggtttcaca actctttaca
     3181 tcgtcatccc ttcttgctac cctttgctga tatccggatg gtcggattga caaagaatac
     3241 gctcttgaag tgggtggtac aatggtcagc atcgtcttcc atccctgtcc tcaaacatta
     3301 aagggtcatt ttttacttca aacccttttg gtgtttattt cccaggtttg tagctaaact
     3361 gttggcaata tagatgatgg accgctattc ggcctggagg aacagggaat aggcaaaagg
     3421 cttcggctgc acgaatctat aatgcacatt gaaagtgatt tccgggctga acatggtcct
     3481 ctggtgtgtt ttgaattttt ataaaatact ttaatttagc gtgctcaata cctgcctttt
     3541 tttgaatatc aacgactaac aaatagtgaa tttagaagaa ataacagcaa gactaaatca
     3601 attgcgtgaa ttgtccggcc aattggatgt gtcggaaagc caaagaaaag ggatgatcgg
     3661 ccttgtagct gaatacacca atcaatttat caatggtttg agcacggtaa gcggattcag
     3721 cgagaagcaa cccggctcat tggcgattgg caatggtgga attcccttag agcaattgtt
     3781 gagctcctat cagagcgaag ttgtcgaaac gggcatcaat gcagcctccg gcacacatct
     3841 gggttatatt cccggtggcg gaatctttgc tgctgcactg gcagatttca tcgccgcagt
     3901 aaccaacccc tatgcagggg tctactatgc ttcaccgggt gccgtcacta tcgaaaacga
     3961 agtaatccac tggctgaaat ccatattttc ctttcctgcc gatgctgttg gcaatctgac
     4021 gtccgggggc tctgtctcca tgctcattgc cttcaatgcc gcccgggata aatatggtgt
     4081 gaagaatgaa aaagtcaggc aaagcgtggt ctatttaagc caacaagtgc accattctgc
     4141 ccagaaggca cttcgaattc taggattgga agatgtcatt atacgatatg ttccgctgga
     4201 cgacaggcac aggatgaacg cagatatatt ggcattgctt gttgcggaag acaagtcaag
     4261 aggtctgcat cctttccttg tcgttgctac agcagggact accgatacag gtgcagtaga
     4321 tcctcttgat gcaattgcgg atattactga aatgcacaac ctgtggttcc atgtcgatgg
     4381 ggcatatggg ggatttttta tcctcacttc caagaaagaa ttgttccggg gtattgaaag
     4441 ggccaattcc atcattgtgg atccccataa aagtcttttc ctcccatatg ggcttggtgc
     4501 tgtcctaatc cgtgacaagg atgccgtgtt gcactccaac cgttatgctg ccaattacat
     4561 gcaagatgct ctgggtgacg aacttgtcaa aagcccatcc aacctgtcac ccgagctgac
     4621 caagcatttc agggggctgc gtatgtggtt gccattgcaa ctccacggcc ttgctccttt
     4681 tatagcctgt ctggaggaga aacttctgct tacggcatac ttcagggcga aaatggtggc
     4741 agtgggcttt aaggtcgggc cggagccgga tctttccgtc agttatttct attatccgtt
     4801 tgaatctgat gcagataaga agaacagcct gttgatgaag gaaatccatg ctgatggaga
     4861 tgttttcctt agttcgtccg taatcgatgg ccggtatgtt attcgtatgg cgattcttgc
     4921 cttccgtacc aaacgtgaaa cgatagaccg tgcaataaca atgatactcc gatgtctcga
     4981 taaagtccgg catgtcggtt aagtgcttac atcagggtat cctgcaggaa gggcaactta
     5041 tccggatagt cagtcgtata atgcagtccc cgactctcat gcctcatcgt tgcactcttt
     5101 ataatgagat aggaaatggt gatgaggttg cgcaactcac aaagctgagg gctgatggtt
     5161 gtcgtgttgt aaagctcttc tgtttccttg tacaacagat acattctgtc gtcggccctc
     5221 ttcagacgga catttgagcg tacaataccc acatagttag acatgatctc gttgacctct
     5281 ttacggcttt gtgtaatgag gaccatttcc ttcggatcgc tggttccgcg tgcattccag
     5341 tccggtattc ccttgcagat cacatacttg tcgatggtgg cgaggatatc catcgctgca
     5401 cggtaaccaa acaccaggcc ttccagcagg ctgttgctgg cgaggcggtt ggctccatgc
     5461 aagccggaat tggtacactc accggctgcg taaagccgat taatggagct tcttccctca
     5521 tgatctatct tgattccgcc acatatatag tgacatgccg gtacaatggg tatcatctgg
     5581 acaaagggat ctatgccgat gctcttgcat ttttcataga tattggggaa atgtcgtaag
     5641 aaaccatcct tatccaggtg tcgacaatcg aggaacatct tatccgcccc cagaatcttc
     5701 atttcgttgt ctatcgccct ggcgactata tctcttggtg ccagactcaa ccgttcatca
     5761 tacttttgca taaattccct gccctgggca tccttgagga tggctccaaa gccccggacg
     5821 gcttcactta tcaggaagtc cggcttgtca ccgcccggat tgtacagcgc agtgggatgg
     5881 aactgcacaa attccatgtt acaaaccctg ccctttgccc ggtaaaacat ggccatccca
     5941 tcgcctgttg ccaccagggg attggtcgtt gtctgataca cctggcctga tccgccggat
     6001 gccaatacgg tctgtctggc caaaaaggtt ttaatctcgt ttgtctttct gtccaatgca
     6061 tagactccgt agcattcgat attgggcgtc aggcgggata cattgtatcc gaggtggtgc
     6121 tgcgtgataa tatcgatggc gaaataatgc tccagaatct ctatgttgtc aaattgggca
     6181 gctttctcca cgacagtgcg ctggatctca cgtccggtaa tgtccttgta atggaggatt
     6241 ctgttttcgg tgtgtccgcc ttccctgcca agatcatatt ctccggtttg agtcttatcg
     6301 aaattggctc cccagtcgat caattcccgg actctttcgg gagcagattt tacaacaatc
     6361 tccaccactt ccttgtcgca tagaccatcc cctgcatcca gggtgtcggc gatatgtttt
     6421 tcaaagttgt ccacctccac atcccataca gtagcaattc caccctgagc aaagttggta
     6481 ttgctttctt tttcggttgt cttggcaaga accgtaatct tcaggtcctt gcgtcgctgg
     6541 gcaacttgaa tggcgaggct gagcccggct atcccggagc ctacgatgac cagatcggaa
     6601 gtttgggcca tgtcggtttg atttttaaaa aaatgatgac aaagatacaa atatagaagt
     6661 tatggaaata ccgccaaaat tcaacctttt tagtcgccgc tgtgtcgcaa ttaacgattt
     6721 ttgcatcttg aaacaacgcc ccattcgggc ttttgtgatg ccggagtatc cgactgtaat
     6781 gactccattc gaagttgaca gataatagaa ggcctcaaag gcttcgtgaa gtaggtagtt
     6841 tttatttcag gctgtacttt gtgttcaaat agagggcatg cttttgtcaa tatacagaat
     6901 gaaatttttc atttcgatag cgatattcat tatgcacatt atttcttaaa taggttcgat
     6961 acttgaatgg caaatattta cctatatttt tcatgccact tggcgaatcc ggatcactcg
     7021 cctgccttga tttttatttt ttggcagtaa aaaaaggatg gtatcaacaa acaatctact
     7081 actccaccaa agcgaccttc atcatattgg tcataccttt ggactttaaa ggcatggagc
     7141 caacaaacac taccgtctca ccactccgta ccctgccttt ggatttcagg atctgcaata
     7201 cttcggtaat ggtttcatcc gtgctattaa agccgtcgta gtggaaacaa cggactcccc
     7261 acacaaggtt gagcatgccg gctatctctt tacgatccga aaagatgaat atttctgccg
     7321 gggagcgata ggacgaaatc ttgaatcctg tatatcccat ggctgtcata ccgatgattg
     7381 cagaggctcc cagttcttct gctgttttag cggcattgaa acacacaaca tctgacatga
     7441 aggtttctga cttgcgcgaa ggataagggc ggatatgatt cttcggatag ttcttttcag
     7501 cttcggcaat gattctgttc atggcgagga ctgtttcaat cggataggcg ccgacactgg
     7561 tctctccact cagcataaca gcatcagtgc cttcgagcac ggcattggca acgtccgtga
     7621 cttcagccct tgtcggtgac ggattggtaa tcatactatc catcatctga gtggccacga
     7681 tgacaggctt tccaaactgg acacataggg ctatgatcct tttttgtatg cccggcagtt
     7741 tttctatcgg catctctaca ccgaggtctc cccttgcgat catgatgcca tctgtcgctt
     7801 tgactatttt ctcaattttt tcgactgcct gaggcttttc aatcttggca atgacgagag
     7861 catgatgttt atgctgtttg attttatctt ttaaggcctt tacctcttta ggccctcgta
     7921 cgaatgataa tgcaatccag ttgaccggct gagtcatcag aaactccaga tcattgagat
     7981 ctttttctgt catcgccggc agggatacca gtgtatcagg aaggtttact cccttattgg
     8041 acgacaattc cttaccatag agaaccttta gtcgtacagt atcgacattg tttgtttcta
     8101 tcacttccag gcccactttg ccatcatcga ttaatacttt ctctcctact tcgacatctg
     8161 cggcgaagtt tttatacgac atgtatatct tttctgcatt accaacacat ttttcattga
     8221 caaaagtcag gatatccccg gcatttagct ccatgacgtt gttctctatc tgcccgacac
     8281 gtaatttagg gccttgaaga tctgcaagga tgccgacatt gattttatag tccttattca
     8341 attttgaaat cgtctcaatc tgccgggctg caacttcgtg agaaccgtgg gacatattaa
     8401 tcctgaatac atccactccg gctttaacca gttctaccat ggtttcataa gaatcacttg
     8461 ccggtccgag tgtagctact attttggtgt tttgaaaact tcgatctgcc ataagacaat
     8521 gataaatgat ttaaggggac aaaattagtg tactttttac aataattact caactcctgt
     8581 aagtatatta taaaaccaag ggacatatca tccctgaatg ggcttttcca ataacttatc
     8641 gggaagtaac tatcctttaa aaagcatcga gtcccgattc taattccata gaaataaaag
     8701 taaccccaag tatgagatga aaaaaatagc agccaattat acaatccggg aaaatgttgc
     8761 tttaagtatt tggtaaaaat acggtggagt gctctcaaac cgccgggcta ttcaatttca
     8821 ggctgacgaa tcagataaaa attctaaact tttactttaa ttcatcttta accacgatgg
     8881 tctgcttgtc gctttgatga ttatcggcgt atttagcaat gatggctgcg atatctgctg
     8941 gcttttccca tttgctgaaa tcggcatcag gcatcgactc tcgattccgg ggggtatcaa
     9001 tgatgcttgg gacgataaca tgggcccgga tgccggtatg cgcagtatct gcattgatga
     9061 tgttggaaag ttggaaaagc agggatttgg aaaggctgta ggctacaaca tgtttaccct
     9121 tcgatgtgtc aagtccctgg ccggaaccca caaaaaacaa cctgcctcca tgattgagct
     9181 tcatatgttc atacaaaggt ttggttagat tgtaggctgt ttcgaaatta aggcgatatt
     9241 gatgctccag ctcactgatg cctgtatcag aaaggtcgcc catggcaaat cctcctgccg
     9301 tcataacagc aatatcgatt ttgccaaatt tttccaacag ttgctttaca cattcctcca
     9361 cggccaactc gtcgagtagg ttgaccgcac actccacata attggcaata tttctgggtt
     9421 gtcctcccga acggtggacc aggccgatta cctgatcccc ccgatccaga tagaaattaa
     9481 ccactgccgt tcccagattg ccggaggcgc cacttacaat cactgtttta tttaccatct
     9541 tttcgtagga gctacttaaa ttccaactac gtaaacaaaa aagaaggatt aaagttatgg
     9601 caatacaaga ggaatcataa cttttggcca ttctccacga tttgagttcg aaaactgaac
     9661 tgggtagaag aataataatt gaccctccag ccggaatcag ggtctcaaaa catcccgaat
     9721 gcaataccat ccatttaaac cattattttg gaatttgtaa tccgggtgtg cctgacatca
     9781 tgctattccg gattattaat ttcctaccta cttcatactg tgggaagatg cggcagaatt
     9841 gaccgatttt atagaaaaaa tgactgtgtt gatggtccct caatttttgt atatccttta
     9901 attaataaca gaccaacaat gcctcgaacg gacttctaaa tacactgttt ctattgctga
     9961 caagcgattg ctccggatgg attgacacga tccggatttt cttccttgtg taatctatga
    10021 atcccgacaa aatttatccg gctcagaatt tgatttttta tataaaatat ctcaaaatca
    10081 atgtgatata tattttattc aataaaacaa tccttggaat tcttgt
//
LOCUS       DAOQMC010000065         9861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2686_length_9861_cov_3.833571, whole genome shotgun sequence.
ACCESSION   DAOQMC010000065 DAOQMC010000000
VERSION     DAOQMC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2686_length_9861_cov_3.833571"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3313
                     /gene="sprA"
                     /locus_tag="PKV31_04035"
     CDS             <1..3313
                     /gene="sprA"
                     /locus_tag="PKV31_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNB92058.1"
                     /translation="EDTRVGIFKNTNIDMRRYKRLKMFVHAEPISEEAPKIPDGDLSI
                     FLRIGSDFTDNYYEYEIPLKVSDLEYLKSVKNDLLVYSEGVWLKDNAFDFPLHILTDL
                     KEERNKVSGAGSYYSKADPEKQSNTITVKGNPNLGYVKGLMIGIRNKQGGIPRCGEVW
                     VNELRMTGFDERGGVAAVSRIDFQLADLGTLTLSGAYSSLGWGGLDQRVQQRAITTNY
                     QYDISTNLELGKFIPGKTGIKIPFYYQYTTNVKTPEYDPYDLDIKLRDKLNQYDGSER
                     DSLREQAIEYENIQSYSFNNVRKERTNKTGTPMPWDIENFSFTYGHSRTKRTDPIIAR
                     DQTDQYKGGLDYNFTMKPLYITPFSKMIKKDKYLKFITDLNFNPLPNTFAFNTQLNRM
                     YSTKLYRFTDPFQSTWRTRNFLWDRSYTLNWDLTKSLKFDFNAVNTSVIDEMNDRYVD
                     SGLPNPNFNSSANRAVIWENIRHLGRNKNYRHTFNLNFNVPFKNIPMMEWVTMRALYG
                     GSYTWSAAAINVDTLGNVIQNTQIRQLNADFSFDRLYSKWNYLKKIEKPESKSTSKIS
                     QPKQKGSNTKPVTDDPGNKDALGKQDSTDAKLSKAERLKLRKEKEKEKRKLAREERRK
                     NYQPSLAERILIRPLLSLRKARLTYTENLNSTIPGYVPSTSSMGMDQWSAPGFGYIAG
                     WQPDDAYFERAVAPNNNWITKNIFQNQLVILGQNQAIDAKINLEPFNDFKIDLNANRT
                     YQNTHTELFKVKAIGEDFGRYAGRDIGSYTTTYFTLNSFFDDMRSVFKRFESNRTVIS
                     NRLGTTPDPNVPGYTTGYGKIQQDVLIPAFLAAYSNTDPTKQNLDIFSTFPKINWQLT
                     YNGLTKLELFKKYFSSVNLTHAYKSTLNVNSFNTSPNYDPEDPYIFNPITNNYYSRLE
                     IPAVSITEGLSPLIRLDVKTKNDITFNIDYKKVRNLSLSFVDNLVNETKSSDITIGAG
                     YTIHSVKMPFMKEFQLKKNDKKKKDKGKDPSKTSTGPGPKGGNKAAENDLIIRLDVSY
                     RDDATVSHFIDQDLTRTSRGAITLRLAPNIEYQYNKNLTLRLFYDYNLTNPKTSQQFK
                     TISQRGGITFRFLLN"
     gene            3631..5499
                     /locus_tag="PKV31_04040"
     CDS             3631..5499
                     /locus_tag="PKV31_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNB92059.1"
                     /translation="MKRFLISTFVIIICWVGTNAQDSLDSLWFRNNYTKMEKMIPMRD
                     GTKLFTSIYIPRDLSGPHPIIMRRTPYSCRPYGLDNYAQFYNGYFRNYLRSNYIIVTQ
                     DVRGRFMSEGDFIDIRPLLAERKSDQDIDEATDTYDTVEWLVNNLENNNGKVGIMGIS
                     YPGFYSTVAALSGHPAIKAVSPQAPVTDWFRGDDFHHNGALALIDAFTFYLNFGRPRP
                     KPTMGSGWSNTQFGPDAYRFFLDAGPLSDIKHKYFGDSIQFWNDLFAHPNLDKFWEDR
                     NPLHHIKDVKPAIMVVGGHFDAEDCYGAWETYKSFARKSPQTNTTLVMGPWFHGNWGG
                     RGSGEQFGPIRFGSKTAEWYQNEFEIPFFKKYLEDNPTPTIGDKVNVFITGSNHWAAF
                     NQWPPEGVEEFRLFLSENNNLRNDKSTQSGVYDQYTSDPSHPVPYRPDISWNRNREYM
                     IEDQRFASQRPDVLTYQTDVLTKDVTMLGPVLADLFVKLTTTDADFVVKVIDVFPDDF
                     NEYTSRDVPMGGYQMLVRGEIFRGRYRKSFSHPEGFKPGKIEQVNFSLPDIAHTFKKG
                     HRIMVQIQSSWFPLFDLNPQQMVDIYRCKKKDFISSTIQIIRSKEYPSSVSFYVKK"
     gene            5766..6605
                     /locus_tag="PKV31_04045"
     CDS             5766..6605
                     /locus_tag="PKV31_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HNB92060.1"
                     /translation="MILEPIERRTNLSREEFKKEYLDPGKPVVIEDLAKDWAGLQKWS
                     FGFFKEKYGDLMVPLVDQSFHDPGARYMEHRNIPFRDYIEAIEKGPTDLRLFLFNIFD
                     KAPELREDFIWPTIMDGFVKSLPFMFFGGAGSITPMHFDIDCPSNFLTHFHTRKKIIL
                     FDRNQSRYLYHQPFTVQSQVDINNPDYDQFPALRKARGIEAILHHGDTVYIPPHWWHY
                     IEYVDSGFSLALRAHDRISNKFVAVQNLIQHSLIDKGMNKILGKKWKNWKVNAARKRA
                     ESA"
     gene            6655..7125
                     /locus_tag="PKV31_04050"
     CDS             6655..7125
                     /locus_tag="PKV31_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNB92061.1"
                     /translation="MIEINNLASQSVRLAVENVLNEGGPFGCIIVRNGEIIATGVNRV
                     TFDNDPTAHAEVMAIRAACTKLHTYQLDDCDLITSCEPCPMCMGAAYWARVKAVYYVA
                     TKADAASAGFDDSFIYEEMEVPYHNRKIPFIRVELNENSAPFEAWLKKENKKLY"
     gene            complement(7152..7958)
                     /locus_tag="PKV31_04055"
     CDS             complement(7152..7958)
                     /locus_tag="PKV31_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB92062.1"
                     /translation="MSYDSRGLFYFARYLPGDMWKLAKSPFQKENLEGLGLVLISTAA
                     LLPFDQYFLDKVRQASRSINLADNTSFGVAVGKGEIILVKYPRNVNSALYQLGEGGTS
                     MYLAGGLWIYGKIARDYRAVQTAGDLVETFIVMGVTTQILKRISGRESPYVSTQPGGA
                     WRPLPSLKEYQSHVPRYDAFPSGHLATMMATVTVLHNNYPEKKWILPVGYSLMGMSAW
                     AMMNTEVHWISDYPLALALGYISGKISTMRHNRSQSEVMKNRVLNPEMTP"
     gene            8356..8985
                     /locus_tag="PKV31_04060"
     CDS             8356..8985
                     /locus_tag="PKV31_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB92063.1"
                     /translation="METLAAISRFKANYGFKKGDIIIKEGQISDGVYFIKRGVVKIEK
                     NGPNGRNFIINICGAGNIIGHRGMQGNTIQKSTVVALSEVTCCFIPVNFFNEILKNAS
                     DLEKQITEDYLCQLEQLEKKSISLAYKSVKEKVAEVILVLATIYNYEPKKQSFSIDLS
                     RQDLADLTGATKEQVSSVLKEFERNSIIRYSGKHFYSLDIGTLKTIAEA"
     gene            complement(8997..9581)
                     /locus_tag="PKV31_04065"
     CDS             complement(8997..9581)
                     /locus_tag="PKV31_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92064.1"
                     /translation="MACKSDHSGHEEELMEISDDTAGHNVGDLVTTDEILDVPEIHRG
                     RIEQIDGKFVIRATALISATLKEDYNNIDGVMDSYPETFTVTVKKTGDTPSSQKDSVV
                     TMKYKTSYIEFRKRKGMQDRLLIASLQNENFMLQNGLHTGIDRAEALAKFGIREQIPQ
                     DLIEVVSSENNDKILLFLNKDTLVKIEIIPSLKE"
BASE COUNT         3185 a         2057 c         1941 g         2678 t
ORIGIN      
        1 ggaagatact cgtgtcggta tctttaaaaa tactaacatc gacatgcgcc gttacaaacg
       61 cctgaaaatg tttgtacatg cagaacccat atctgaagaa gctccaaaaa ttccggatgg
      121 cgacctgagt atcttcttgc gaattggctc cgacttcacc gacaattatt acgaatatga
      181 aattccgctt aaggtttctg atctggaata tctgaaatcc gtgaagaatg acttgctggt
      241 gtacagtgaa ggtgtatggt tgaaagacaa cgcatttgac ttccctttgc atatccttac
      301 tgacttgaag gaagaacgaa ataaagtaag tggagcaggt tcttattatt caaaggccga
      361 tcctgaaaaa caaagtaata ccattactgt aaaagggaat ccaaaccttg gttatgtaaa
      421 gggactgatg attggtatca gaaacaaaca aggaggtata ccacgatgtg gtgaagtatg
      481 ggtcaatgaa ctaaggatga ctggattcga tgagcgagga ggtgtcgcag cagtttcacg
      541 cattgatttt cagttggctg acttagggac ccttaccttg tcaggagcat attcgtcact
      601 gggatggggt ggattggatc agcgtgtaca acaaagggcc atcactacca attaccagta
      661 tgatatctct accaacctcg aactgggcaa gttcataccc ggaaagacag gaataaagat
      721 tccattttat tatcaataca caacaaatgt caaaacaccg gagtatgatc catatgatct
      781 ggatatcaaa ctccgcgaca agctgaatca gtatgatggt tcagagcgtg atagtcttcg
      841 tgaacaggcc atagaatatg aaaatataca atcatactct ttcaataacg taagaaagga
      901 acggactaat aaaacaggca caccgatgcc ttgggatata gaaaacttta gttttaccta
      961 tggtcattcc aggacaaagc gtactgaccc gattattgca cgtgaccaga ctgatcaata
     1021 caaagggggg ctggactaca actttacaat gaagccattg tacattactc cgttttccaa
     1081 aatgatcaag aaagataaat atctgaagtt tatcactgat cttaacttta atccgctgcc
     1141 gaacactttt gcattcaata cgcagctcaa cagaatgtat agtacaaagc tataccgatt
     1201 tacagatccg ttccaaagta cctggagaac caggaatttc ctttgggaca gatcatacac
     1261 actcaattgg gatctgacga aatcgttgaa atttgatttc aatgcagtaa atacctctgt
     1321 aattgacgaa atgaatgata gatatgtcga ttccggcttg cctaacccca actttaattc
     1381 cagtgccaac agggctgtaa tatgggaaaa tatacgccat ctcggaagaa ataaaaatta
     1441 tagacatacc tttaacctga actttaatgt tccattcaag aacattccaa tgatggaatg
     1501 ggtaacaatg agggcattgt atggtggaag ttatacctgg agtgctgcag caatcaatgt
     1561 agacacctta gggaatgtca tccagaatac acagatcaga cagttgaatg ctgattttag
     1621 tttcgatcgg ttatattcaa aatggaatta cctgaaaaaa attgaaaaac ccgaaagtaa
     1681 atcaacgtca aaaatttcac agccaaaaca aaaaggctca aacaccaagc ctgtaaccga
     1741 tgatcccgga aataaggatg cattgggcaa acaggattct accgatgcca agctttcaaa
     1801 ggcagaaagg ctaaaactca ggaaagagaa agaaaaggaa aagagaaagc ttgcccgtga
     1861 agaacgaaga aaaaattatc aaccctctct agccgaaagg attttgataa gaccactgct
     1921 aagcttgcgc aaggcacgtt tgacctatac ggaaaatctg aactccacga ttccgggcta
     1981 tgtcccttcg acttcctcga tgggaatgga tcaatggtcg gcaccgggct tcggttatat
     2041 agcgggctgg caaccggacg atgcttactt tgaaagggct gtagcaccca ataacaactg
     2101 gattaccaaa aatatcttcc agaaccaact ggttatactt ggccagaacc aggccattga
     2161 tgctaaaatc aatctcgagc cattcaatga tttcaaaatc gacctgaatg ccaatcgtac
     2221 ctaccagaat actcatactg agctattcaa ggtaaaggca attggtgaag actttggtag
     2281 atatgcaggt cgggatatcg gttcatatac aacgacatac ttcaccctca actccttctt
     2341 cgatgatatg agaagtgttt tcaaacgatt tgaaagtaac agaactgtga tttccaatag
     2401 attgggaaca acaccggatc caaacgtacc gggttatact acaggctatg gtaaaattca
     2461 acaggatgta ctcatcccgg ccttcctggc cgcatactcc aatacggacc cgacaaaaca
     2521 gaatctggat atcttcagta ccttccctaa gatcaactgg caattgacct acaacggtct
     2581 taccaagtta gagttgttta aaaaatactt ctcaagtgtc aacctgaccc atgcatataa
     2641 gtcaacctta aatgtcaaca gtttcaacac ttcgcccaac tatgatccgg aggatcctta
     2701 tatcttcaat ccgataacaa acaattatta cagtaggctg gagattccgg cggtatccat
     2761 cacggaaggg ttgtctccat tgattcgttt ggatgtaaag acaaagaacg acatcacatt
     2821 caatattgat tacaagaaag tccgtaatct ttcactctca ttcgttgata atctggtcaa
     2881 tgagaccaag tcatccgata tcacaattgg tgccggatat accatccata gtgttaagat
     2941 gccttttatg aaggaatttc agcttaagaa aaatgacaag aaaaagaagg ataaaggaaa
     3001 ggacccatcc aaaacatcta caggaccagg ccctaaaggt ggtaataaag cagccgaaaa
     3061 tgacctgatt atccggttgg atgtatctta tcgggatgat gcaacagtca gtcattttat
     3121 agaccaagat cttaccagaa catcgcgcgg agctataaca ttaaggctcg ctccaaacat
     3181 cgaatatcaa tacaataaga acctgaccct ccggctgttc tacgattata accttaccaa
     3241 cccgaaaact tcacaacaat tcaaaacaat ctcccagcga ggtggtatca cgtttaggtt
     3301 cctgctcaat tgacaactct gaatattaat atccaaaggc tgcaaaattg atttgcagcc
     3361 tttagtattt tttggagccg cacccaaaca agagacttga ttgtgacaaa agccacctta
     3421 tgctaagacc tgttagtatt gaaagatcta caggtaaaat ttacacaaaa tcaaatattc
     3481 cgtaattccg aaagaaatcc tatttttgaa aatcattggt ttcttataag acgaacagaa
     3541 ggaattgaat ctgcaagcca ctactgccca ttcacaggct atgcagttat ttccgaacat
     3601 ttatctatat aaatttcaat ttctgtattc atgaaaagat tcttgatttc gacttttgta
     3661 atcattatat gttgggttgg gacaaatgcc caggattcat tggattcact ttggtttagg
     3721 aacaactaca cgaagatgga aaaaatgatt ccaatgaggg atggaaccaa actttttaca
     3781 agtatctata tccccaggga cctttccgga ccacatccca tcatcatgcg tcgtactccc
     3841 tattcctgca gaccttatgg cttagacaac tacgctcaat tttataacgg ttacttcagg
     3901 aattatctaa gaagcaacta catcattgtc acacaggatg taagaggaag gttcatgagc
     3961 gaaggcgatt ttatcgatat ccgtccccta ctcgctgaaa gaaagtctga tcaagatatt
     4021 gatgaagcaa ccgatactta tgacaccgtc gaatggcttg tcaataatct ggaaaacaat
     4081 aatggcaaag ttggaataat gggaatttca taccccggct tctactccac tgtagccgca
     4141 ctgtcaggac atcctgccat caaggctgta agtccacagg ctccggtcac tgactggttt
     4201 cgaggagatg acttccatca taacggagct ttggcattaa tcgatgcatt taccttttat
     4261 cttaatttcg ggcgaccaag acctaaacca accatgggca gtggatggtc caatacccag
     4321 tttggaccgg atgcttatcg attctttctc gatgcgggac ctctttcaga tattaagcat
     4381 aaatattttg gagattccat tcaattttgg aatgacctgt ttgctcatcc aaatctggac
     4441 aagttctggg aagacagaaa ccccttacat catattaagg atgtcaaacc ggccatcatg
     4501 gttgtaggag gacatttcga tgctgaagat tgttatgggg cttgggagac ctacaaatct
     4561 tttgctcgaa aaagtcccca gactaatact accctggtca tgggcccttg gttccatggc
     4621 aattggggcg gacggggcag tggagaacaa ttcgggccaa ttcgtttcgg ttcgaaaacg
     4681 gcagaatggt accagaatga atttgaaatt cccttcttta aaaaatattt ggaagataat
     4741 ccgactccta ccattggaga caaggtcaat gtatttatca ccggctctaa ccattgggcc
     4801 gcctttaacc aatggcctcc cgaaggtgtt gaagaattca gattatttct ctcggaaaat
     4861 aacaatctcc gcaacgacaa atcaacccaa tcaggagttt atgaccaata tacaagcgac
     4921 ccatcacacc ctgtacctta caggcccgac atcagttgga acagaaacag ggaatacatg
     4981 attgaagatc aaagatttgc ttctcaacgg ccggacgtac tgacttatca aacagatgtc
     5041 cttaccaaag atgtaactat gttgggaccc gttctggctg atctttttgt taaattgact
     5101 acgacagatg cagatttcgt cgtcaaggta attgatgtat ttccggatga tttcaatgaa
     5161 tatacgagca gagatgtacc gatgggaggt taccagatgc tggtcagagg tgaaatattc
     5221 agaggtagat accgcaaatc attctcccac cctgaaggtt tcaaaccggg aaaaatagaa
     5281 caggtaaatt tcagtttgcc tgacattgct cataccttca aaaagggaca tcgcatcatg
     5341 gtacagatcc aaagtagctg gttcccactc ttcgacctca atccacaaca aatggttgat
     5401 atatatcgat gtaaaaagaa agacttcatc tcatctacta tccagatcat ccgtagcaaa
     5461 gaatatccgt catcggtctc tttttacgtc aaaaaatgaa ttcgcccggg gaaaagtatc
     5521 acatagtctg acagaaatat atttcaaacg tagtatctct cccgaagtgg acacaaaaag
     5581 acagctaatc gaagtaaaga atggtcgata attggcaata taactttgaa tgaatgatgc
     5641 aattaatgca atttaacata ttgtttaata aaataaaaaa cctgaacaat ttgaaattcg
     5701 ttacatcaaa aggatgcttg acatcaaata ggtaatcgtt ttgtttaacc ctattactaa
     5761 aagaaatgat tcttgaacca atagaaagaa gaacaaatct atccagggaa gaattcaaga
     5821 aagaatacct ggatccaggg aagcctgttg taatagagga tcttgccaag gattgggcgg
     5881 gtctgcaaaa atggtcgttc gggtttttta aagaaaaata tggagatttg atggtcccat
     5941 tagtggacca gtccttccat gacccaggtg ctcgctacat ggaacacagg aatataccgt
     6001 tcagggatta cattgaagcc atcgagaaag gcccaacaga cctgcgtctt tttttattca
     6061 atatcttcga caaggctccg gaacttagag aagatttcat ctggcctact atcatggacg
     6121 gatttgtcaa gtcactgccc tttatgtttt tcggaggagc aggaagtata acgccgatgc
     6181 attttgatat agattgccct tctaattttc tgactcattt tcataccagg aaaaaaatca
     6241 tcctctttga cagaaatcaa tccaggtatc tttaccacca gccgtttacc gttcaaagtc
     6301 aagtggacat caacaatccg gattatgatc agtttccggc acttcgcaag gcacgtggaa
     6361 ttgaagccat actccaccat ggtgatacag tgtacatacc ccctcattgg tggcattata
     6421 tcgaatatgt agatagtggc ttttcccttg ccctgagagc acatgacagg atatccaata
     6481 aattcgtcgc cgttcaaaat ttgattcaac attcgttgat cgacaagggc atgaataaaa
     6541 ttctgggtaa aaaatggaaa aactggaagg tcaacgctgc acgtaagaga gcggaatctg
     6601 cttgattaat atcaggacat attgtccaaa accaattgaa gaacaaaata aaaaatgatc
     6661 gaaatcaaca atctggcatc ccaatcggta cggttagcag ttgaaaatgt tttgaatgaa
     6721 ggaggaccat tcggttgtat tattgtcagg aatggtgaaa tcatagcaac aggtgtcaac
     6781 agggtgacct tcgataatga tcctactgca catgcagagg ttatggctat cagagcagcc
     6841 tgcacaaagt tacataccta ccagttggac gattgcgacc tgataacctc ttgtgagccc
     6901 tgtcccatgt gcatgggagc tgcatattgg gcaagggtaa aagctgttta ttacgtggct
     6961 actaaggcag atgccgcctc tgccgggttt gatgactctt tcatttacga agaaatggag
     7021 gtcccttatc acaatcgcaa aatcccgttt atccgggtgg agctaaatga aaatagtgcc
     7081 ccgttcgagg cttggctaaa gaaggaaaat aaaaaactct attgaaaata tattgccttt
     7141 agaacttgaa atcaaggtgt catctccgga ttaagaaccc gatttttcat aacttccgat
     7201 tgcgacctgt tgtgtcgcat ggtagaaatt ttgccggata tatatcccaa tgccaaggct
     7261 aaaggataat cactaatcca gtgtacctct gtattcatca tagcccaggc ggacataccc
     7321 attaatgaat atccaacggg taaaatccat ttcttctcag gataattgtt atgtaaaacc
     7381 gtcactgtag ccatcatggt agccagatgg ccggatggga aagcatcata ccttggcaca
     7441 tgagattgat actctttcag ggatggcaaa ggcctccatg ctcctcccgg ttgagtcgat
     7501 acgtaggggc tttcccttcc tgaaattcgt ttcaggatct gagttgtaac tcccatgaca
     7561 ataaatgttt ccaccaaatc acctgctgtc tgaaccgctc tgtaatccct ggcgatctta
     7621 ccataaatcc acagcccccc ggcgagatac atacttgtac caccctctcc aagctgatat
     7681 aaagccgaat tgacatttcg aggatacttt acaagtataa tctctccctt accaactgca
     7741 acaccaaagg aagtgttgtc tgccaggttg atactccggg atgcctgccg aaccttgtca
     7801 aggaagtatt ggtcaaatgg aagaagagca gcggtcgaaa tcaaaaccag tccaagacct
     7861 tccaggtttt ctttttgaaa aggagattta gccagtttcc acatatcgcc ggggagatat
     7921 cttgcaaagt aaaaaagacc gcgtgaatca taagacatca ccttgtcatt gtgattgata
     7981 tataaagcat tcgaagaagt atcaaaatgt ctggaaaatc cattctgatt gaaatttgaa
     8041 attaccctcc cctggcccac tgattttata tgaagcaaac agagcattcc cagaatgaag
     8101 cacgtccaaa aattgttata aacccgcata tccaaatatg tcattttgaa attatacgtc
     8161 aaaaataatc tataaaaaaa ataagtcaat ctgaatattt tcgtaaattt aagccgatga
     8221 cattaatgtt gtatcatact ttgtatggat gacctgtcat tttttaaaat ataaacctgt
     8281 caaaatatga ccccctcagg taatacttgt ctgacttgta gagtaaaaca ttgctcgatt
     8341 ctcaaacctt gttcgttgga gactttggca gccattagca ggttcaaagc caattacggt
     8401 tttaagaaag gagatataat cattaaagaa ggtcaaatct ccgatggggt atattttatt
     8461 aagagaggag ttgtgaaaat tgaaaaaaac ggaccaaatg ggcgtaattt cataattaat
     8521 atatgcggag ccggtaatat cataggtcat cgcggtatgc aaggtaatac cattcaaaaa
     8581 agcacagttg tcgccctaag tgaagttaca tgttgtttca tcccggtcaa tttttttaat
     8641 gaaatcctta aaaacgcatc tgatcttgaa aaacagatta ccgaagatta tctctgccaa
     8701 ttggaacaac tggaaaagaa atcaattagc cttgcttata agtctgtgaa ggaaaaggtg
     8761 gctgaagtaa tacttgttct ggcaactatc tataattatg aacccaaaaa acaaagtttc
     8821 agcatcgact tgtccagaca ggatctggca gacctgacag gagcaacaaa agaacaggtt
     8881 tcatccgtac tcaaggaatt tgaacgaaac tcaatcatca gatattcagg caagcatttc
     8941 tattccttag atatcggaac cctgaagacg attgcagaag cataatttat agctttttat
     9001 tcctttagag aagggattat ctctatcttg accaaagtat ctttgttgag gaacaaaagt
     9061 atcttatcat tattttcaga acttaccact tctattaaat cctggggaat ctgctctctg
     9121 attccaaatt tggcaagagc ctcagcacga tcaatacctg tatgaagccc attctgcaac
     9181 atgaaatttt cattctgaag acttgctatc aacaacctat cctgcatacc tttcctcttt
     9241 ctgaattcaa tataggaagt cttgtatttc atcgtgacca cagagtcttt ctggctggaa
     9301 ggagtatcgc ctgttttctt tactgttaca gtgaaagttt ccggataaga atccattaca
     9361 ccatcgatgt tattataatc ttccttcaaa gtcgcagaaa tcaatgctgt agcacggata
     9421 acaaactttc cgtcaatttg ctctatcctt ccccggtgaa tctcgggcac atcaagtatt
     9481 tcatctgttg ttaccaggtc acctacatta tgccctgcag tgtcatcact gatctccatc
     9541 aattcctctt catgaccgga atgatccgac ttacatgcca taattagcat aagtccacaa
     9601 aaaatgaaat atttatacat taatcttaaa gattacgtga tgcaaaaata caattttccc
     9661 cttgaataaa tctgatattt ttcaccgcct actatttaag tacaaacatc tagccatttt
     9721 tattgttgta agttgcatga gtagagtagc gatagatctt caatcaggag atattaaaaa
     9781 ggaagaacgg ttgattttgg gtatcgacct gggtacaaca aacagcctta tcacatatat
     9841 caaagatggg aagccattgg t
//
LOCUS       DAOQMC010000066         9856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2689_length_9856_cov_4.213244, whole genome shotgun sequence.
ACCESSION   DAOQMC010000066 DAOQMC010000000
VERSION     DAOQMC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2689_length_9856_cov_4.213244"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2577)
                     /locus_tag="PKV31_04070"
     CDS             complement(<1..2577)
                     /locus_tag="PKV31_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNB92065.1"
                     /translation="MFKNLLFIRFRYLFCFVSLLSLNIPNQATGQIFEDFSGAEFNSS
                     GLWTGDLSNFTVSNGQGRLNAVVAGSSYFSSPIVWQDSFVFSFYVKLNFAPSTQNKIS
                     IYLWNNQADLNGGSGLMMNIGKNGDDDGVTLVYGNQTSSTTISESGSGEFASGVDGIF
                     SGIITEERLYIQYKKVGQTSSVLYDGPKPDAFMLDQSAYFGFLMKYSSGNIKNFFFDD
                     IEIDRYKADTVGPVVVSVVPTGQSSVRLIVNEEIVEEDFRNPENYKMYDSFGRELTIQ
                     HVATSGLTADITITETFKPDVYFLTISGLHDLAGNLMNKQTLKFNYFAYATPERYDVL
                     INELMADPSPVVTLPESEYIELWNTSSKILLISQLKIVKGITFYDLPKRALAPDEYLI
                     IVPSGDVDKWNGYNNVVGISPFPTLNNDGAMLTLIDSAGNLIHHVSYDLSSYKDNSKK
                     DGGWSLEHTVKEGICDGNDVWRASTDASGGTPGRVNSNTVIASENVLLTGMELLDDRN
                     LQLCFSKYLNFSDSVTQAAFSSVNTPSFQIFFSQEVDNCCIIEFEESLMDAVWYELGI
                     SGYIVNCLGNYIEADTLIGFGWGFPKPGPNDIIINEILFDPNTGGSDFVEFYNRTSTT
                     FDLRGLNIVNTQGNKISTINKTALLKPNDYLAFCPDKNWLAANYTLRNPQNIIEIGIP
                     SFNSDQGNVSLMDGISLIDSFRYNQKLHNVFLTKVKGVSLERVSADGDSNQKSNWQSA
                     ASDAGGATPGYVNSQTRTLKNVDKILTKPEDHFSPNNDGFLDQFIVQYLLDKPGYILR
                     WRVFDAAGRLIKSDQSGVLTGTDGFITWDGSTDDLKKALPGIYVFKFQFTHPDGTTKS
                     EHI"
     gene            2744..3757
                     /locus_tag="PKV31_04075"
     CDS             2744..3757
                     /locus_tag="PKV31_04075"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNB92066.1"
                     /translation="MKVAVCGVTGLVGSVMCKVLEERNFPVDEFIPVASDRSIGKEVV
                     FKGKSYKVVGMSDAVAMRPDLALFSAGGATSLEWAPKFAAVGTTVIDNSSAWRMDKDI
                     KLIVPEVNANTLTAQDKIIANPNCSTIQMVVVLKPLHDKYGIRRVVVSTYQSVTGTGV
                     KAVSQMMNERKGIEGEMAYAHRIDMNVIPQIDVFLDNRYTKEEMKMVNETKKILSDEN
                     IRVTATTVRIPVVGGHSESVNIELKNEFRLDDIFEILRNTEGVIVQDDPFNAVYPMPK
                     DSHEKDEVFVGRIRRDESQPNTLNLWVVSDNLRKGAATNAVQIAEYLMEKQLLGKQMA
                     TTL"
     gene            4003..5478
                     /locus_tag="PKV31_04080"
     CDS             4003..5478
                     /locus_tag="PKV31_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92067.1"
                     /translation="MKSKLVIWGQQDDRKVLLALSLRALDNKVDVYVFPEEIATEELF
                     LNLSNNWKNGTEIQFPEGHQFFERELTASGNLLPEGITTGSEDLISRAQTEWQFFVLS
                     ARLSASYEAELESIKDKIEQLKEFSQPIWNELKTFWDKVQGQIKEKNIFADHISSLRK
                     ATNQAFDDLKEKRKELDRIFNEKSGLVKENFSKSLNEIEEKISKGLSLHPIFEELKDL
                     QNKFKTAALNNADRKSIWDKLDSLFKQVKEKRFGGSDKGSSDSAKERLDNRYNGLMAA
                     IAKMEQSIQFDKNDLEFQTKKMDGQVGQLELQIRQAKTKMIEERIQSKQAKLNDMLST
                     KAELEQRKLKLERSEAMRTEKEEAKKLVQEKIAAEIKAASESRAENSEDLEKAAKMIS
                     EQKNQKKEEKKDESILSSIATNLSETLEDAVDTIAAVSSVIGEKLGDIAESISEKAED
                     ILENITASKEEEEEAKTETEENDDIAADSLTDNTEQEKTES"
     gene            complement(5479..7875)
                     /locus_tag="PKV31_04085"
     CDS             complement(5479..7875)
                     /locus_tag="PKV31_04085"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNB92068.1"
                     /translation="MTQLEQGSNKKYICIHGHFYQPPRENAWLEEIQSQPTASPFSNW
                     NERIAYECYSPNRAARILDNEGNILYIVNNYEKISFNFGPTLLSWLEEHDADTYLEII
                     EADRKSVKENNGHGNAIAQIYNHIIMPLASEAEKELQVFWGVRDFVFRYGRNPEGMWL
                     SETAVDTATLIALRRNGILFTILAPRQADAVKEKNGEWILCSEKAVDTGKPYKVILPD
                     GDSIAVFFYDGSISQKVAFEGLLNDGVAFASMLMQAADQKDYDALISIATDGESYGHH
                     HRYGEMALSSCIYHIGQHPDYILTNYAAYLAMFPPVDEVRIKENTSWSCYHGVERWRA
                     DCGCNTGSHPRWNQKYRQPLRDSLNWLSNQLSRLFNDKNKLFYNAHDALLNYIDVVLD
                     RSEVSTEYVLRKYVIRPDSESNGVDALKLLEMMRHANLMFTSCGWFFDEISGLETTQI
                     LQYARRAITHAEYFGLFLEDGFRQRLELCPTNLPEYSSGKDVYETVVRPSEIRLLQVA
                     MHMSVLSVFEEVPSEMTLYKFFANHETFVREEKGDQKLVIGHAVMKSVLTRAELEYNF
                     SVLVYKGIDLIGYVKQGAPDEGFNYFIRTAKELFQQDELDKLSGYFRDYFKTGSFDFS
                     NLFKDEKINLLNIISKRNIKNAVASFRNIFNVNYPLLYEMEKVGFPISKGFLEIVAFV
                     FNADIIDELSVSGDVDIAKLRQLTADLKKWKVPIEDPERIAFLAERQIFAQIRRLNEN
                     IKNPIPHELIDFLLDWVYGLNLPINLWQSQNEFYEISKQISMHPDIKLEQIAKKLKFA
                     "
     gene            complement(7872..9821)
                     /gene="glgB"
                     /locus_tag="PKV31_04090"
     CDS             complement(7872..9821)
                     /gene="glgB"
                     /locus_tag="PKV31_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNB92069.1"
                     /translation="MNGGVVPFSLFTEYDIHLFRNGLHYKLYNLLGSHLVVVNGVKGV
                     YFAVWAPSASNVTVMGDFNYWRTSQYRLNPRWDNSGIWEGFVPGVKQGAIYKYNITGP
                     DGKVYEKGDPYAFRWEVPPGTGSIVWDIKDYSWRDKSWLEGRKKFNKLQSPISAYEVH
                     LGSWKKPERKETSFLTYKQLKDELVPYVKEMGFTHVELMPVMEHPYYPSWGYQIIGYY
                     ATTSRFGSPQEFMELVDAFHQEGIGVILDWVPSHFPSDGHSLSYFDGTHLYEHGNPQL
                     GYHPDWKSLIFDYGKKEVKSFLISNAVFWCEMYHADGLRVDAVASMIHLDYSRKDGEW
                     TPNKHGGRENLEAIDFIKEMNQAVHAYHPDVITIAEESTSFPFVTAPVNDGGLGFDEK
                     WMMGWMHDTLKYFALDPIHRQFHHDQITFSMVYAYSEKFVLPFSHDEVVHGKGSLLTR
                     MPGDEWQKFANLRAMLGYMFTHPGAKLLFMGGEFGQSWEWSHINGLEWHLLRFAYHSG
                     IQGFVKELNRLYTTYNALYYHNFHPAGFEWLSVNDRENSVLIFLRKGKTEVDKLIIVL
                     NLTPVIREHYRFGVPDKGSWRELLNSDDKKWGGSGVHNKSAIRATKKAEHNRDYSISI
                     TCPPLSIVVFEPVRVVKKTVKKGKP"
BASE COUNT         3164 a         1996 c         1916 g         2780 t
ORIGIN      
        1 gatatgttcg cttttggttg taccatccgg atgtgtaaac tggaacttaa agacgtagat
       61 tcccggcaat gctttcttca gatcatcagt gcttccatcc caggtgataa agccatctgt
      121 cccggtcaac actccggact gatctgattt aatcagccgg cctgctgcat caaatacgcg
      181 ccaacgtaat atatagccgg gcttatcaag aaggtattga acaataaatt gatcgagaaa
      241 accatcatta ttgggggaaa aatggtcttc aggctttgtc aatatcttat caacattttt
      301 caaggtcctt gtttggctgt tgacatatcc cggagtggca ccaccggcat cggaagcagc
      361 ggattgccaa ttggattttt ggtttgaatc tccatccgca gaaactcgtt caagggaaac
      421 acccttaact ttagttagga atacattatg caacttttgg ttgtacctaa aactatcgat
      481 aagtgaaata ccatccatta aagaaacatt gccttggtca ctattaaacg aagggatgcc
      541 aatttcaata atattttgtg ggtttctgag tgtataattt gcagccagcc aatttttatc
      601 aggacaaaat gcaagataat cgttgggttt aaggagagct gttttgttga ttgtagaaat
      661 tttatttccc tgggtattta caatattcaa ccctcttaga tcaaaggtag tagaagtcct
      721 gttatagaac tccacgaaat cggatccacc ggtatttgga tcaaacaaaa tttcattgat
      781 aataatatca tttggtccag gttttggaaa accccagcca aaccctatca aagtatctgc
      841 ttcaatgtaa tttccaagac aattgactat atatccggaa atccctaatt cgtaccatac
      901 cgcatccatc aaagattctt caaattcaat gatacaacaa ttatccactt cctgagaaaa
      961 gaatatctgg aatgatggtg tattgaccga agaaaaggca gcttgggtca ctgaatctga
     1021 aaaatttaaa tatttagaaa agcacaattg aaggtttctg tcatcaagta attccattcc
     1081 tgtcaataaa acattttcag aagcgataac agtgttggag ttgactctgc cgggcgtacc
     1141 acctgaagca tccgttgaag cccgccagac atcattacca tcacaaattc cttccttaac
     1201 ggtatgctca agactccagc ctccatcttt tttcgaatta tccttgtagg acgataagtc
     1261 ataacttaca tggtggatca aattaccggc agaatctata agggtgagca tcgctccgtc
     1321 attattgagt gtagggaagg gtgaaatgcc gacaacattg ttatatccat tccacttatc
     1381 aacatctccg gaagggacga tgatcagata ttcgtcaggt gctaaagccc tcttgggcag
     1441 gtcgtagaag gtgatacctt tgacaatttt aagttgactg atcaacagaa tcttactact
     1501 cgtgttccac aattcaatat attcgctttc aggcaaggta acaaccggcg atggatctgc
     1561 catcagttca ttgatcagga catcatatct ttctggtgtt gcataggcaa aataattgaa
     1621 tttcaatgtc tgcttattca taagatttcc tgccaggtca tgcaaacctg agatagtcag
     1681 aaaatagaca tccggcttga atgtttcagt aattgtaata tccgcagtca aaccagaagt
     1741 ggcaacatgc tgaatagtta attcccttcc gaaactatca tacattttat aattttcagg
     1801 atttcgaaaa tcttcttcaa caatttcttc attaactatt aatcttaccg aggactgtcc
     1861 ggttggaact actgaaacaa ctaccgggcc aacggtgtcg gctttatatc tgtcaatttc
     1921 aatatcatca aaaaagaaat tcttaatatt accggatgag tatttcataa gaaagccgaa
     1981 ataagcagac tggtccagca taaaggcatc cggcttcggg ccgtcataca gtactgatga
     2041 ggtttgtcca acctttttat attgaatata caggcgttcc tcagttatga tgccggaaaa
     2101 aatcccatca acgccggaag caaattcccc ggagcctgat tcagatatgg tagttgaaga
     2161 ggtttggttc ccataaacca aagtgacgcc atcgtcatct ccattcttac caatattcat
     2221 catcaaacca gatcctccgt tcaaatcggc ctgattgttc caaagataga tgctaatctt
     2281 attttgagtg gatggggcaa aattcagttt aacataaaat gaaaaaacaa aagagtcttg
     2341 ccatacaata ggtgaagaga aataggatga accggcaaca actgcattca ggcgcccctg
     2401 tccgttactg actgtaaaat tgcttaaatc cccggtccaa agtccgcttg aattaaattc
     2461 cgctcctgaa aagtcttcaa aaatctgacc tgtcgcctga tttggtatat tcagtgaaag
     2521 taaggaaaca aagcaaaaca aataacgaaa acgtataaaa agtaaatttt tgaacatcag
     2581 tagtttaata aaattattgg ttgcaaatca aaccgattat ctatctttac aggttgtaaa
     2641 tttaattttt ttatgatcaa aattggtctt tgaaaggaat taaatattcc ggaaaggctg
     2701 attttataaa agtaatcatt caaattataa aattatcgaa tatatgaaag ttgctgtttg
     2761 tggtgtaaca ggattggttg gaagtgtgat gtgtaaagtc ctggaggaaa gaaatttccc
     2821 ggttgatgaa ttcataccgg ttgcaagtga ccgttccata gggaaggaag ttgtgttcaa
     2881 gggaaagtca tacaaggtag ttggaatgtc tgatgctgtg gcaatgagac ctgacctggc
     2941 tttattttca gcaggggggg cgacatcgct ggaatgggcg ccaaagtttg cagctgttgg
     3001 cactactgtt attgacaact cttcggcctg gcgaatggat aaagacatca agctcattgt
     3061 cccggaagta aatgccaata cattgacagc ccaggataag atcattgcca atcccaactg
     3121 ctcaaccatt caaatggtag ttgtattgaa accacttcat gacaaatatg gtatcaggcg
     3181 ggtagttgtt tcaacctatc agtctgtaac aggtaccggg gtaaaagcag tctctcaaat
     3241 gatgaatgag cgcaagggca ttgagggtga aatggcttat gcacacagga tagatatgaa
     3301 tgtcattcct cagatcgacg ttttcctgga caatcggtat accaaggagg aaatgaaaat
     3361 ggtcaatgaa accaaaaaga tattatccga cgaaaatatt cgcgtaactg caactacggt
     3421 gaggatccct gtagtcggag gacacagcga gtctgttaat attgaattga aaaatgaatt
     3481 ccggttagat gatatatttg aaattcttcg gaatactgaa ggtgtaatcg tccaggacga
     3541 cccattcaat gctgtgtatc ccatgcccaa agattctcat gaaaaagatg aagtttttgt
     3601 aggcaggatt aggcgagatg aatcccaacc caacacactt aatctttggg ttgtatcgga
     3661 taatttgcga aagggtgcag ctaccaatgc cgtccaaata gctgaatatc tgatggaaaa
     3721 acagctttta ggtaaacaaa tggcaacaac gctctaacct attgaaaaat caggtatggt
     3781 taaattgctt aaaaactatt taagatacat ttaatcaaat aattaaaaat aaagaattaa
     3841 taaaaagtca ggaatattat ttctcaatcg aaataaattt acgtttcttt gtgttaagat
     3901 acctgtaata tctttaatgc aatgaacgaa aagttgcatt attacgtcaa taattataca
     3961 aatgttaata tcaatcgcaa gattgtagaa aattaggtac gaatgaaatc aaaattggtt
     4021 atttggggtc aacaggacga ccgtaaagta ttacttgctt tgtcattaag agcactggac
     4081 aacaaagtcg atgtgtatgt atttccggaa gaaatcgcaa cagaagaatt gtttctgaat
     4141 ctatccaaca actggaaaaa cggtacagaa attcaatttc ctgaaggtca tcagtttttt
     4201 gaaagagaac tgactgcatc gggcaatctt ttacctgaag gaataactac aggaagtgag
     4261 gatttaattt caagagcaca gactgaatgg caattctttg tactttctgc cagacttagt
     4321 gctagctacg aagctgaact tgaatctatc aaggacaaaa tagagcaatt gaaagaattc
     4381 agccagccta tatggaatga gctaaaaaca ttctgggata aggttcaggg acaaataaaa
     4441 gaaaagaata ttttcgcaga tcatatttca agtctaagaa aggccaccaa tcaagctttt
     4501 gatgatttga aagaaaaacg taaggaactg gaccgaatct tcaatgaaaa atcaggatta
     4561 gtaaaggaaa atttttccaa atccttaaac gagattgaag agaaaatatc caaaggtctt
     4621 agcctccatc caatatttga agaactaaaa gacttgcaaa acaagttcaa gacggccgca
     4681 ttgaacaatg ctgatcgtaa gtcaatatgg gataagctgg attccttgtt caaacaagtt
     4741 aaagagaaac gattcggagg ctcggataaa ggttcatcag actcagcaaa agaaaggctt
     4801 gataaccgat ataatggatt aatggcggca atagctaaaa tggaacaatc catccaattt
     4861 gacaagaatg atttggagtt ccagactaaa aagatggatg gtcaggtagg acaacttgaa
     4921 ttgcagatta ggcaggccaa gaccaaaatg attgaagaaa gaatccagtc aaagcaagca
     4981 aagctaaatg acatgttgtc cacgaaagcc gaacttgagc aaaggaaatt aaagcttgaa
     5041 agaagtgagg ctatgcggac cgaaaaggaa gaggctaaga aattagtaca ggaaaagata
     5101 gcagctgaaa taaaagctgc cagtgaatca agagctgaaa attctgaaga cctagagaag
     5161 gctgccaaaa tgatttctga acaaaaaaat cagaaaaaag aagagaaaaa ggatgagtcc
     5221 atattgtctt ccatagcaac taacttaagt gaaactcttg aagacgcagt agatacaata
     5281 gccgccgtca gttccgtcat tggagagaaa ctgggagata ttgctgaaag catctctgag
     5341 aaagcagaag atatacttga aaatattaca gcgtctaaag aggaagagga agaggcaaaa
     5401 acggaaacag aggagaatga tgatattgct gctgattccc tgactgacaa tactgagcag
     5461 gaaaaaactg aaagctgatc aggcaaattt taattttttc gctatttgtt ccagctttat
     5521 atcagggtgc attgaaatct gtttgctgat ttcataaaat tcattctggc tttgccacag
     5581 attaatagga agatttaatc cataaaccca atccaacaag aaatcaatca attcatgtgg
     5641 gattgggttt tttatattct catttaatct cctgatttga gcaaatatct gacgttctgc
     5701 caaaaaggct attctttccg gatcctcaat tggcactttc catttcttca aatcagctgt
     5761 caactgtctt aattttgcaa tatcaacatc accggatacg gataattcgt caatgatatc
     5821 cgcattgaag acaaaggcaa ctatttccag gaaacctttt gaaatcggga atccgacctt
     5881 ttccatttca tacaataatg gataattgac attgaatata tttctaaaac tggcaacggc
     5941 attttttata tttcttttgg aaataatatt taataaattg attttctcat ctttaaataa
     6001 attcgagaaa tcaaaacttc ccgttttaaa ataatcccta aaatatccgg atagtttatc
     6061 caattcatcc tgctgaaata attccttggc tgtccggata aaataattga atccttcatc
     6121 gggcgcccct tgtttaacat aaccaatcag atcaatacct ttataaacca atacggaaaa
     6181 attgtattcc aattcggccc ttgtaaggac cgatttcatc acggcatggc caatgaccag
     6241 tttttggtct cctttttcct cacggacaaa ggtctcatga ttggcaaaga acttgtacaa
     6301 ggtcatctcc gatgggacct cctcaaatac tgataataca gacatatgca tggccacctg
     6361 cagcaatcgg atttcagaag gacgtacaac agtttcatat acatccttgc cggaactata
     6421 ttccggcagg ttggtaggac aaagttcaag cctttggcga aagccgtctt caaggaataa
     6481 tccgaaatat tccgcatgag taatggctct gcgtgcatat tgaaggatct gtgtggtttc
     6541 cagaccactg atttcatcga aaaaccagcc acaactggtg aacatcaggt tggcgtgtcg
     6601 catcatctca agaagtttga gtgcatcgac tccattactt tctgaatcag gccggatgac
     6661 atattttcga agaacgtact ctgtacttac ctcactccta tccaaaacaa cgtcgatata
     6721 gttcagcaag gcatcatgag cgttataaaa aagtttgttt ttatcattga ataatctgct
     6781 taattgattt gaaagccagt tcaggctatc gcgtagtggc tgtctgtatt tttgattcca
     6841 cctgggatgg ctgcctgtat tacatccaca atctgcacgc catcgttcta ccccgtgata
     6901 gcaactccaa ctggtatttt cctttatcct gacttcatcg acaggaggaa acatggctaa
     6961 gtaggcagcg taatttgtca aaatataatc cggatgttgc ccgatatgat agatacagga
     7021 cgaaagtgcc atttcgccat agcgatgatg gtgaccataa gattcaccat ctgtagcaat
     7081 actgataaga gcatcataat ctttttggtc tgctgcttgc atcaacatag aagcaaatgc
     7141 aacaccgtcg ttgagcaaac cttcaaatgc aaccttctga gaaatggacc cgtcataaaa
     7201 aaatacagca atagaatcgc catccggcag aataaccttg taaggtttcc cggtgtcaac
     7261 ggccttttca ctacaaagta tccattcacc gtttttttct ttaacagcat cagcttgtct
     7321 tggtgcaaga atggtaaata agattccgtt tctacgcaat gcaattagtg ttgcagtatc
     7381 gacagctgtt tcactgagcc acattccctc cggattcctc ccatatcgga atacgaaatc
     7441 acgaacaccc caaaatacct gcaattcttt ttcagcctca cttgccagag gcataatgat
     7501 atggttgtat atctgtgcta tagcattgcc atgtccatta ttctctttga cactcttacg
     7561 atcagcctcg atgatctcaa gataagtatc agcatcatgt tcttccagcc atgataacag
     7621 agtcggtccg aaattaaaac tgattttttc ataattattt acgatatata agatattacc
     7681 ttcattatct aaaattcgtg cagctctatt gggagaataa cattcataag cgatcctttc
     7741 attccagttg gaaaatggag atgcggttgg ttgagactgg atttcttcaa gccaggcatt
     7801 ttctcttgga ggctgataga aatgcccgtg aatacagata tactttttgt tggagccctg
     7861 ttctaactgc gtcacggttt ccctttttta acagtttttt tgacaactct taccggttca
     7921 aaaacaacta tagacaatgg aggacatgtg atagatatcg aataatcccg attatgttct
     7981 gcttttttgg tggctctaat agcggatttg ttatgaactc cactcccgcc ccacttcttg
     8041 tcatcactgt tgagcaattc cctccagcta cctttatctg gaactccaaa acgataatgc
     8101 tcccggatca caggagtcag gttaagaacg atgattagtt tgtcgacttc cgtttttcct
     8161 tttcggagga atattaggac actgttctcc cggtcattga cactgagcca ttcaaaaccg
     8221 gcagggtgaa aattatgata atataatgca ttgtaagtcg tataaagccg attcaattct
     8281 tttacaaagc cttgtatgcc tgaatgatag gcaaatcgaa gcaaatgcca ttccaggccg
     8341 ttaatgtgac tccactccca gctttggccg aattccccac ccataaacaa cagtttagct
     8401 ccgggatgtg taaacatata gccaagcata gctctcaaat tggcaaattt ctgccattca
     8461 tcacccggca tacgggtcaa tagacttccc ttgccatgaa ctacctcatc gtgcgaaaat
     8521 ggcaggacaa acttttcaga gtaggcatat accatactga aagtaatctg atcatggtga
     8581 aactgacgat ggataggatc aagtgcaaaa tacttaagag tatcatgcat ccagcccatc
     8641 atccactttt catcaaaacc gagaccacca tcattgaccg gtgcagtaac aaagggaaac
     8701 gaagtcgatt cttcggcaat cgtaattaca tccggatggt aggcgtgtac tgcctgattc
     8761 atctccttga tgaagtcaat cgcttcaaga ttctcacgtc caccatgctt attgggtgtc
     8821 cattctccat cctttctgga ataatcaagg tgaatcattg aagcgacagc atcaaccctc
     8881 aaaccatctg cgtggtacat ttcacaccaa aaaacagcat tactaatcaa aaaggattta
     8941 acctctttct ttccataatc gaatatcagg ctcttccaat caggatgata gcctaactga
     9001 ggattgccat gctcatacaa gtgagtgccg tcaaaataac tcagtgaatg tccgtcactc
     9061 ggaaaatggc tgggtaccca gtcaaggata acaccaatac cctcctgatg aaaggcatcg
     9121 accagttcca taaactcttg cggacttcca aatcgtgagg ttgtagcata atacccgatt
     9181 atctggtatc cccaggaagg atagtatgga tgctccatta ccggcatcaa ttcaacatgt
     9241 gtgaagccca tttccttgac atagggcacg agttcatctt tcagttgttt ataggtaaga
     9301 aaactggttt cctttctttc cggcttcttc cagcttccta aatgaacttc gtatgcagag
     9361 atcggagatt gcagtttatt aaactttttt ctcccttcga gccatgattt gtccctccag
     9421 gaatagtctt ttatatccca gacaatactg ccggtgccgg gaggaacttc ccaacggaaa
     9481 gcatagggat cgcccttttc atagacctta ccatcaggtc cggtaatgtt gtatttgtaa
     9541 attgcacctt gctttactcc ggggacaaac ccctcccaga taccgctatt atcccaacgt
     9601 ggattaagcc tgtactgtga agtacgccaa taattgaaat cgcccattac agtaacattt
     9661 gacgcagaag gcgcccatac agcaaaataa acgcccttta cgccattaac aacaacaaga
     9721 tggctcccca gtaagttata caatttataa tggagcccat tcctgaataa atgaatatca
     9781 tattcagtaa aaagagaaaa gggtacaaca ccaccattca tagcaacacg gttttattga
     9841 taaatgtaag aataaa
//
LOCUS       DAOQMC010000067         9583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2777_length_9583_cov_4.436398, whole genome shotgun sequence.
ACCESSION   DAOQMC010000067 DAOQMC010000000
VERSION     DAOQMC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2777_length_9583_cov_4.436398"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKV31_04095"
     CDS             complement(<1..434)
                     /locus_tag="PKV31_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB92070.1"
                     /translation="MKLSRRSLIRKLSIGMGVMSINLPGFANEFRDETREKKVNSSNS
                     FNMSGYAAPKMDRVRVGIIGLGNRGEEAVRRLCYIDGLDIVALCDKYSDRTEKCRTAV
                     VDKKLPDPKTFSGSEDAWKGLCDMDLDLVYICTPWHWHTPMA"
     gene            complement(457..1449)
                     /locus_tag="PKV31_04100"
     CDS             complement(457..1449)
                     /locus_tag="PKV31_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92071.1"
                     /translation="MFLFPWALFAQEDPEALVLRGVALYDQGKYPEALKVLERARDIW
                     PRSSTILYEIALTRYSLRDYEGCHRLTDSIIVMRDNNMTQGYLLKASALKQLGKTIES
                     SEVLIDGIRDTGGDEQLFYNLGINFITEKKFNEAEICFLNAINFKPTHSSSYLQLAEL
                     NQDVNPTKALLAACQFLLLEPNSNRSKPAFELIEQILNGKSKSPSMKIQKEDAENHII
                     RLDSSYIFSNGIIRLDAETNFNNNGIVDFDYFSAELKKVSLNCIDRIEYKEHDVWRGY
                     LIPFFADIFSSGFGDVFCNYIAQSRWPACSLWLNNRRSEFDRFTAFLKSEEEKK"
     gene            complement(1573..2811)
                     /locus_tag="PKV31_04105"
     CDS             complement(1573..2811)
                     /locus_tag="PKV31_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB92072.1"
                     /translation="MNQIENIRQAFKNIRSNKVRSILTILIIAFGIMALVGILTAIDS
                     ILNSMVTNFSGLGANSFSITQKNADFRSNRRGFREKPGKEINFAQAMMLFEKNDMPTT
                     ISISATGINGATVVYNGKKTDPVISIAGINENYMKVNALDLATGRAFSANEFLYGENV
                     CIVGNEVIKRLFDGRESSALDRVISAGGNRFRIVGVMTTQGSSMTSNMDNQIEIPLLT
                     QKKLYGYSDQNYNIAVSVPQGLNLDEAIDYTTGLFRNIRQLRVGQDNDFEIQQADAIM
                     NIIQENTVTIRIATIGIGLITLLGAAIGLMNIMLVSVTERTREIGILKALGATRNNIL
                     IQFLTEAIVICQLGGLVGIVLGILAGNSIALFLDGGFIIPWAWITLGIITCLLVGLAS
                     GLYPAMRAARLDPVESLRYE"
     gene            complement(3244..4566)
                     /locus_tag="PKV31_04110"
     CDS             complement(3244..4566)
                     /locus_tag="PKV31_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNB92073.1"
                     /translation="MKRIVYFLVMIMTSLQPSMSFTQETVNDKMQWFRDAKLGIFIHW
                     GIYSVDGVDESWSFYNHKLPYEKYMEQLKGFTARNYKPADWVKLIKETGARYTVLTTK
                     HHDGVALWDTKANDLSVVKLTPSGRDVLTPFTKEVRKAGLKLGLYFSILDWSHPDYPG
                     FLKDSNRYAIKDDSVRWNRYLRFYQKQIKELSNDYNPDLFWFDGDWEHSADEWQADKV
                     RANILEHNPRTIINSRLTGRGDYDTPEQNFPVTRPERVWELCMTCNNNWGYHPDDTNY
                     KTPYEVITIFADAISNGGNLLLDIGPREDGWIPDEQVHILKELGKWNKKHAEAIFGTV
                     AGLPQGHYYGASTLSKDSTTIYLFVPAKDAGKICIKGLVNPIKDIRIIGSDIHPGHKI
                     VGKISWSHVPGLVFIDNLPETALDEYMTVVEIKLDGKLKLYRGTGGFQ"
     gene            4762..5262
                     /locus_tag="PKV31_04115"
     CDS             4762..5262
                     /locus_tag="PKV31_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92074.1"
                     /translation="MDEKILFKSFLSFFAEADFPLILSYDIDEPESFSDDPIPEGLAR
                     IFIAEEEGEDAFNEYVPVVQFRLNKNYLACIVWKASLQGYEFILIIYDNEGNIQESDS
                     VAGTYHIHEPMIYKIAEFASPNRAVVVEGNIDTRNKDANLPPARKFILNISDIGEINY
                     SLVGNL"
     gene            5264..7426
                     /gene="rnr"
                     /locus_tag="PKV31_04120"
     CDS             5264..7426
                     /gene="rnr"
                     /locus_tag="PKV31_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNB92075.1"
                     /translation="MTKKYPKSFKGRKLNTLELKIIVNRELKNLNGKSYSAAGLIKKL
                     KISNNRDEVQLILEKLVKEGRVRQTTGGVYQSNNKSSIPEKLFIGKVDLIRSGAAYII
                     VEGKSDDIYIAPGDLMGALDGDTVEVSVVRRKGRQRSDGRVIRIIRRSREIFMGRIFV
                     NKKYSVVTPFKTPDGFEILVFNEGLNGAEDGDIVTVRVISWPDGKNKMIKGEITEVLS
                     ADNASDMEMKSILIGQGFDLHFSKETLKEAHAIPDSIPEQEIMQRRDMRDIATFTIDP
                     ATAKDFDDALSYFLLEDGNMEIGVHIADVSHYIKPGGPMDKEAYERSTSVYLVDRVLP
                     MLPEKLSNELCSLRPNEDKLTFSAVFVFDKDTKVVSRWFGKTIIHSDRRFTYEEAQEI
                     IESGVGEYVEELMQLNTIAKKLRDIRYAKGAISFESDEVQFRLDENGVPVEIFIKERK
                     DAHLMVEEFMLLANRSVAEYIHQKGASGEIPFIYRVHDRPDNQKLSEFATFAAELGFK
                     MDLSKPAAVIHSFNLLAKKAEEDETLRLITPLAIRTMAKAEYSTNNIGHFGLGFDDYT
                     HFTSPIRRYSDVLAHRILFRNLNSAIYREDKSLLDVFCKHISAQERKAMEAERESVKY
                     KQVEFMSRFVGTEFNGIISGMHDRGVFVELDDNHCEGMIRFDRFDESFILDDSKMKAF
                     GRISGRTIKIGDRIRIKVLNADLDNRQLDFAPVMNQEA"
     assembly_gap    8067..8166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8665..9147
                     /locus_tag="PKV31_04125"
     CDS             8665..9147
                     /locus_tag="PKV31_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB92076.1"
                     /translation="MSKLFIAISNLLIIVMFMSACAGGSDSSNATEAATEPKTFDTES
                     YDPKRGEGKFNAENVKVEGLDAAMAAKGKSISETKCFSCHKTTDERLVGPGWKGVTAK
                     HSPFWIMNFITNPDPMIDKDPELQAQLEICLVRMPNQNLNDQEAREILEFMRENDGAK
                     "
     gene            9351..>9583
                     /locus_tag="PKV31_04130"
     CDS             9351..>9583
                     /locus_tag="PKV31_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92077.1"
                     /translation="MSSIKLSIVSTLFLALMVVSCKPKGTTNAISGNAAQKTYVAPGK
                     YDEFYNFVSGGFSGQLSVYGLPSGRLLRVIPVFS"
BASE COUNT         2813 a         1907 c         1880 g         2883 t
ORIGIN      
        1 atagccatag gcgtatgcca gtgccatggt gtacatatat acacaagatc aaggtccata
       61 tcacagagtc ccttccaggc atcctcgctt ccactgaagg ttttgggatc cggcaatttt
      121 ttatcgacga ccgctgtcct acacttttca gtgcgatctg agtatttatc acataaggca
      181 actatatcca gtccatctat gtagcacaga cgtctcaccg cttcctcgcc acgatttccc
      241 aggcctatga tgcctacacg tactctgtcc atcttagggg ccgcatagcc cgacatatta
      301 aaactatttg aactgtttac tttcttttca cgagtttcat cccgaaattc attcgcaaac
      361 ccgggcagat tgatgctcat gacacccatt ccgattgaaa gtttcctgat tagacttcta
      421 cgtgataatt tcatctttct ttattttaaa aatgatttat tttttttcct cctcactttt
      481 caaaaatgcc gtaaatcggt caaattcact acgccggttg ttgagccaca gggaacaagc
      541 tggccatcgt gattgggcta tataattaca aaatacatct ccgaatccac tgctgaatat
      601 atctgcaaag aaagggatca gatagccgcg ccacacatca tgctccttgt attctattct
      661 atcaatacaa ttcaaggaaa ctttttttag ctcagcacta aaataatcaa aatccactat
      721 tccattatta ttaaaatttg tttctgcatc aagtcgtatg attccattgg aaaaaatata
      781 gctactgtct aacctgataa tatgattttc agcatcttct ttttgaattt tcatggaggg
      841 cgatttggat ttgccattca ggatttgttc aatcaactca aaagctggtt tggatctatt
      901 ggaattcggt tcgagtaata aaaattgaca agctgccaat aatgccttag tgggatttac
      961 gtcttggttc aattcagcta attgaaggta tgaagaggaa tgagttggct tgaaattgat
     1021 tgcatttaaa aaacatattt ccgcttcatt gaattttttt tcagtaataa aattgattcc
     1081 aagattgtaa aaaagttgtt catctccccc ggtatccctg atgccatcaa tgaggacttc
     1141 cgaagactct atggtcttac caagttgctt taaggctgat gctttgagga gatagccctg
     1201 tgtcatatta ttgtctctca tgactattat gctgtcagta aggcgatgac aaccttcata
     1261 atccctgagg ctatatcttg ttagagctat ttcataaaga atagtcgatg atcttggcca
     1321 gatatcgcgg gcccgctcca ataccttcaa tgcttcgggg tattttccct gatcatagag
     1381 ggcgactccc cgaagcacca aagcttcggg atcttcctga gcgaaaagtg cccatggaaa
     1441 gagaaacaat aaattgagaa tatacttcat taccaatgaa aacgaagcgg aaagttaaca
     1501 tatttcttcc gaccattgaa ggttcagaaa caatctttac tatccttttt ctttttctta
     1561 agagaagttt ctttattcat atctcagact ctccaccgga tcaagcctgg cggctcgcat
     1621 cgcagggtaa agccctgatg ccaaaccgac taaaagacaa gtgatgatac cgagtgttat
     1681 ccatgcccaa ggaatgatga agccaccatc gagaaataac gctatggaat ttccggccaa
     1741 aattccgagc acaataccga ccaggccacc cagttgacag atcactattg cctcagtcaa
     1801 aaattgtatg agaatattat tacgtgttgc acctaatgct ttgagaatgc caatttctcg
     1861 ggttctttcc gtgacagaga cgagcataat gttcatcaag ccgatcgctg cacccaataa
     1921 ggtgatcaac cctatgccga tggttgctat tcttattgta accgtatttt cctgaatgat
     1981 attcatgatg gcatcagcct gttgaatctc aaagtcattg tcttgcccaa ctctgagctg
     2041 cctgatattt ctgaataatc ctgtggtgta gtctatagcc tcatcaagat tcaagccttg
     2101 gggaacactg acagcaatat tgtaattttg gtctgaataa ccgtagagtt ttttttgtgt
     2161 aagtaacggt atttctatct ggttatccat attggaggtc atggaacttc cttgtgttgt
     2221 catcactccg acaattctga atctgtttcc tcctgccgaa atcactcgat caagtgctga
     2281 tgattccctt ccatcgaaaa gcctttttat tacctcattc ccaactatgc acacattttc
     2341 gccataaagg aattcattgg ctgaaaatgc tcttccggtt gcaagatcca acgcatttac
     2401 cttcatatag ttctcattaa tgcctgcaat agagatgacc gggtctgttt tctttccgtt
     2461 ataaactact gttgcaccat tgatgccggt agcagaaatg gaaattgtag tcggcatgtc
     2521 gtttttctcg aataacatca ttgcctgagc gaagttaatt tccttaccag gtttttccct
     2581 gaatcctcta cgattgcttc ggaagtcagc atttttctgg gtgattgaaa aactgttagc
     2641 tcctaatcct gaaaagttag tcaccatgga attcaatatg gagtcaatag ctgttaggat
     2701 tccaaccaat gccataatcc cgaatgctat gatgagaata gtcaatatgg accgaacctt
     2761 gttggaacga atgtttttaa aagcttgcct tatattctca atttgattca aatcttgttt
     2821 ttcctctttt gacgttctaa aggtaaaatt ataagatgga actataaatt acttttgccc
     2881 aaatgtcaat tttctccgat aaatgttcat tcatttagcc tttaaatttc ttttacagtt
     2941 taatcggatt taaaataatc attcggaaag ccgggtacaa aaagctttga tttagtattg
     3001 aaagcttctg attccgctgt gtcgtgcagt ttagtgtagg gggaaatcga agtaggtatg
     3061 aaattaatag acagaaatcc agaaatgaaa agcattatac tttgggtata gatcagatta
     3121 agcagaaaaa cccgaagtaa gataaaatga attcactgaa actttctttc cctatggtca
     3181 atcgattatt agttttctac ctaattcatc ataagccgca aatctctctc ccctcatgct
     3241 gtatcattga aagcctcccg tgccccggta taattttaac ttcccatcga gcttaatttc
     3301 gacaactgtc atatactcgt cgagcgcagt ctccggtagg ttgtctataa aaacaaggcc
     3361 cggcacatga ctccaggata tttttcctac aattttgtgg ccgggatgaa tatcagagcc
     3421 tatgattcgg atatccttga ttggattgac aaggcctttt atacatattt tacctgcatc
     3481 ctttgccggc acaaataaat aaatcgttgt agagtccttg gaaagcgtgc ttgcaccgta
     3541 ataatgtcct tgtggcaaac ctgccacagt cccaaagatc gcctccgcgt gctttttatt
     3601 ccacttgccc agttccttca ggatatgcac ttgctcatca ggtatccagc catcttcccg
     3661 tggaccaatg tccaacaaaa gattaccgcc attggatatg gcatcggcaa atatagtgat
     3721 gacttcatag ggtgttttgt agttggtatc atccggatga tatccccaat tgttattgca
     3781 ggtcatgcat aactcccaga cccgctcagg tctggtgaca gggaagtttt gctcaggagt
     3841 atcgtaatca ccacggcctg ttagccttga gttgatgatg gtccggggat tatgctccaa
     3901 aatatttgcc cttaccttat ctgcttgcca ttcgtcagca ctatgctccc aatcaccatc
     3961 aaaccaaaac aaatccggat tgtaatcatt actcaactcc ttaatttgct tctgatagaa
     4021 ccttagatac cgattccatc gcacactgtc atccttgatc gcatatctat tactatcttt
     4081 cagaaaaccc ggatagtcag ggtgactcca gtctaagatt gaaaaatata accccagttt
     4141 cagcccggct tttctcactt cctttgtaaa tggagtcagg acatccctgc ctgatggagt
     4201 gagcttgact acactaagat cgttggcttt tgtatcccac aaggcaactc catcatgatg
     4261 cttggttgtg aggacagtat aacgcgcacc ggtttccttg atcagcttta cccaatcagc
     4321 cggtttatag ttccgtgcag taaacccctt gagctgctcc atatattttt cataaggcag
     4381 tttgtgatta taaaacgacc aactctcatc cacaccatcg acagaataaa ttccccaatg
     4441 aatgaagata cctaatttag catcgcgaaa ccattgcatt ttatcattta cagtttcttg
     4501 agtaaaggac atggaaggct gaaggctggt cataatcatg actaaaaaat aaactattct
     4561 tttcatgaat aagtcaaaca ttacttggga gttaataatt cggctaaaat aactattttt
     4621 ccttacagga aataatttag tcaattatta cctatatatg gtcaggaatg tcaaataatc
     4681 aattttttcg caacttttgc caatggaaat ttgttcctcc tcaatgattt aggtgtaaaa
     4741 cttaaaattc tactttctac catggacgaa aaaatacttt tcaaatcatt tctttctttt
     4801 tttgccgaag ccgattttcc tttgatttta tcttacgata tagatgaacc ggaatcattt
     4861 tctgatgatc cgatacctga aggactagcg agaatcttca tcgcggagga ggaaggcgag
     4921 gacgcattca atgaatatgt tcctgttgtg cagtttcgac tgaataagaa ttatctggcg
     4981 tgtattgtat ggaaagcaag cttacaaggc tatgagttta ttctaattat atatgataat
     5041 gaaggcaata ttcaggaatc cgattctgtg gctggcacct atcacatcca tgaacctatg
     5101 atatacaaaa ttgctgaatt tgcttctccc aaccgcgctg tagtagtcga aggcaatatt
     5161 gataccagaa ataaggatgc caatttgcct ccggcaagaa aatttatctt aaatattagt
     5221 gacatcggag aaatcaatta ctcccttgtt ggaaatctgt aatatgacaa aaaaatatcc
     5281 caagtccttc aaaggaagga agttgaatac actggaacta aagatcatcg ttaatcgtga
     5341 attgaaaaat ctcaatggca aatcatactc tgctgcgggc ttgataaaaa aacttaaaat
     5401 cagtaacaac cgcgatgaag tccaactaat tttggaaaaa ctggtaaagg aaggcagagt
     5461 tagacagaca accggaggtg tttatcaaag taataacaaa tcatccattc ctgaaaaact
     5521 ttttattgga aaggtggatt tgattcgttc tggagccgcc tatataatag tagaaggaaa
     5581 atccgatgat atctacatag ctccgggaga cctgatgggc gcacttgatg gcgatacagt
     5641 tgaggtatca gtggtaagaa ggaaaggccg acaaagatcc gacggcagag ttatacgtat
     5701 catacgccgc tcaagggaaa tattcatggg ccgaattttc gtcaataaaa aatatagtgt
     5761 tgtcacccct ttcaagacac ctgatggttt tgagatatta gtcttcaatg aaggattaaa
     5821 tggggcggaa gatggtgata ttgtgactgt tcgtgtcatt agctggcctg atggtaaaaa
     5881 caaaatgatt aagggggaaa tcacggaagt gttaagtgct gacaacgcct ccgatatgga
     5941 gatgaaatct attttgatag gccaaggctt tgatctgcat ttttcaaaag aaacgctgaa
     6001 agaggctcac gccataccag acagcatccc ggaacaagaa ataatgcagc gcagagatat
     6061 gcgcgatatt gcgacattta ctatagaccc tgctacagcg aaagattttg acgatgctct
     6121 ttcatatttt ttactggagg atggtaacat ggagatagga gtacacatcg ctgatgtttc
     6181 tcattatatc aagccgggtg gcccgatgga taaggaagca tacgaacgca gcacatccgt
     6241 atatcttgtc gatcgtgttt tgccgatgct gccagaaaag ttatccaatg aattatgttc
     6301 cctgcgaccc aatgaagaca aactgacatt ttctgctgta tttgtttttg acaaggatac
     6361 aaaggttgtt tcccgatggt ttggtaagac aattatacat agtgacaggc ggtttactta
     6421 tgaagaggct caggaaatca tagaatccgg tgttggggaa tatgtagagg agttgatgca
     6481 acttaacaca atcgccaaaa agctacgtga tatacgatat gccaaaggag ccatatcgtt
     6541 cgagtcggat gaagttcaat tcagattgga tgaaaatgga gttccggtgg agatctttat
     6601 taaggaaaga aaggatgccc atctgatggt tgaagagttt atgttgcttg ccaatcgatc
     6661 tgtagctgag tacatccatc aaaagggagc aagtggcgaa attccattta tttaccgcgt
     6721 acatgaccgc ccggataatc agaaattgag tgaatttgcc acatttgctg cggaattggg
     6781 attcaaaatg gacctttcca aacctgcagc cgtcatccat tcatttaatc tcctggctaa
     6841 aaaagctgag gaggatgaaa cgctgagact gattacacct cttgccatac gcacaatggc
     6901 gaaagccgaa tattcaacca acaatattgg ccatttcggt cttggctttg atgattacac
     6961 ccactttact tctccaattc gtagatatag tgatgtcctg gcacaccgaa tcctgtttcg
     7021 gaatctgaat agcgccattt atagagagga caaatcgctg ctggatgtat tttgtaagca
     7081 tatttcagct caggaacgaa aggcgatgga agcggaacgt gaatccgtta aatacaagca
     7141 ggttgagttt atgagtcgat ttgtcggtac tgaattcaat ggaatcattt ccggaatgca
     7201 cgaccgtggt gtgttcgtgg aactagatga caatcattgt gaggggatga tacgattcga
     7261 ccggtttgat gaatctttta ttctggacga cagtaaaatg aaggcatttg gtaggatttc
     7321 aggccgaacc atcaagattg gtgatcggat cagaattaag gtgcttaatg ctgatcttga
     7381 caacagacag ttggatttcg cgcctgtaat gaaccaggag gcttaaaaat agacatcaac
     7441 cagggagggt gcatcgccgt aggttatact ttatcaaaga tttgaatggt ccaagagtaa
     7501 atttttaaag attaattcag atggtcaacg gatagcaaca gagattgaca ggttaccatt
     7561 cctcgatatc ggaggattgg aggaaagaca tcagggtaaa ggtattttgt ggtttcccaa
     7621 agtaggtaga tgattaataa ttaccttgtc aatagaattg ttcttctcca ggtaccttta
     7681 tgtaaattct attcaagatt gagcatattt tttaatcgtg ccgggtgtat ggtgcattat
     7741 acctcagtcg acggttatta aacatgtaca tacttcattt tttttccaaa aaataaccaa
     7801 acacccacta ctgcaacact cacctgaagt aggtagatga ttaatagtca ccgtgtcaat
     7861 aaaattgctc ttttccaggt gcctttaagt aaattctatt caagattgag catatttttt
     7921 aatcgtgccg ggtgtatggc gcatgatacc tcagtcgacg gttattaaac atgtacatac
     7981 ttcatttttt tccccaaatg taaccaatca cctactgcaa cactcacctg aagtaggtag
     8041 atgattaata gtcaccgtgt caataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnntatg gcgcatgata cctcagtcga cggttattaa acatgtacat acttcatttt
     8221 tgcttttctg cctaatctca ttttctattt ttctttttga agctatccgg ccaatccccg
     8281 tttaattatc tttaattcgt aaattttttg gacaaagccg tcagttattc cctgtttcca
     8341 acacaaggac taatggactc aggaagaaat aattcctgaa aaatattgcg ccttgtaaaa
     8401 aaaatattga cggagtttga aaaaacccca aaaaaatcaa acccaaaata tgttggacgg
     8461 tatggccttt agggttatat tatcttgttt ttcacaaatt agcacaatat aattgcatct
     8521 tttgcctaaa tgataaaata tttacaagta agtttttttt gactgtaaat ctctgcagtt
     8581 actaattttt catttatgac tgtggtcata aaaattgacc caaaagaaga gtcaattttg
     8641 acaaaaattt aaaacttgtt aaagatgtca aaacttttta ttgcgatttc caacctgctc
     8701 attattgtga tgtttatgag cgcttgcgca ggtggatccg acagcagcaa tgcaacagaa
     8761 gctgctactg aacccaagac tttcgatacc gagagttatg acccaaaaag aggggaaggt
     8821 aagttcaacg cagagaatgt gaaagttgaa ggtcttgatg cagcaatggc cgccaaggga
     8881 aaatccattt ccgaaactaa atgcttttct tgtcataaga ctactgatga gcgtttggtg
     8941 ggaccaggat ggaagggagt tacagccaag cattcgccgt tctggatcat gaactttatc
     9001 acaaatcctg atccgatgat tgacaaagac cctgaacttc aggcacaatt agagatctgt
     9061 ctggttagga tgccgaacca aaatctcaat gaccaggagg caagggagat tcttgagttt
     9121 atgcgtgaaa acgacggcgc aaaataagtt aaggagccgg tattgatacc ggtgaattca
     9181 tatagattta ttccatattt cggcttttaa tttgccgtat ccgggtggta gcacaactcg
     9241 aaatgggaat gcattgtgcc caaagtatgg gttttatgaa tcttttcaat tatgtttaga
     9301 acggtatcaa tttcccgata atatttcaaa actcattaat attttttaat atgtctagta
     9361 tcaaattgtc aattgtatca acacttttcc ttgcgttgat ggttgtttct tgtaaaccaa
     9421 aaggaactac caatgctatt agtggaaatg ctgcacaaaa aacttatgtg gcgcccggta
     9481 agtacgacga attttataac ttcgtatccg gtggattcag cggccagctg agtgtttacg
     9541 gccttcctag tggtaggctt ttaagagtta tccccgtttt ttc
//
LOCUS       DAOQMC010000068         9571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2781_length_9571_cov_4.942413, whole genome shotgun sequence.
ACCESSION   DAOQMC010000068 DAOQMC010000000
VERSION     DAOQMC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2781_length_9571_cov_4.942413"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PKV31_04135"
     CDS             <1..394
                     /locus_tag="PKV31_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92078.1"
                     /translation="ISDILVEGYKLSSKGKEVSKRFAKNIDGLKICFKTNQNELVEAG
                     REKFFIRIINPTGETVSVLNAGSGVIKASEDGAEIPYSLLKECNYENADAVACANWQP
                     NFQFIKGSYEIHVYNKGYMAGKSTFTLK"
     gene            complement(564..1451)
                     /locus_tag="PKV31_04140"
     CDS             complement(564..1451)
                     /locus_tag="PKV31_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNB92079.1"
                     /translation="MKTDFKKIFLYLMGVALISIASITLHDAFEQEPLPQAIKSPSIN
                     RPFDFAGELVPVANQDVRDRLDKELVMNTYLHGSTLLNLKMAGRYFPLIERILMEENI
                     PDDFKYLVPVESNFSNATSPAGAKGFWQLMPSTAKELGLIVNDKIDERYNVELATRAA
                     CKFIKTLYNRFGNWTLVAAAYNGGGGRIDRELKAQKVNNFYDLYLNPETSKYIFRILA
                     IKEVMSSPSNFGYDIDQVELYKPLTTTRQIEVTGAIPDLTAFAIENGSTYRMLRYLNP
                     WIIDRDLPATGKKLIILLP"
     gene            complement(1470..1880)
                     /locus_tag="PKV31_04145"
     CDS             complement(1470..1880)
                     /locus_tag="PKV31_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB92080.1"
                     /translation="MKDFLDLLGRIFIATIFIYEAYDSIYYFHMTKATMSMYGLTWRQ
                     DLLLVGAIILLVLGGLMVLIGYRSSFGAILLILYYLPVTLIVHSFWNDPVKVQRIQAI
                     MFMKNIAIMGGLLMIIVNGSGRYSIKRLLATLKV"
     gene            2303..2956
                     /locus_tag="PKV31_04150"
     CDS             2303..2956
                     /locus_tag="PKV31_04150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB92081.1"
                     /translation="MYLVKTPSFVKRFIPLALWGIRPGEKKIFLTFDDGPIPEVTPWV
                     LDRLDEFDAKATFFCVGENIHKHPEVFNDVIERGHGVGSHTYNHLNGWETDNLEYLLN
                     VKKAAQMSHSVLFRPPYGKLKPKQAQFLHKHYQIVMWDVLSGDFDQNISKEQVYKNVV
                     NNAVDGSIIVFHDSLKAKERMEYALPRVLEYFSEKGFSFAAIQENEAYSPALVPQYA"
     gene            complement(3080..3619)
                     /locus_tag="PKV31_04155"
     CDS             complement(3080..3619)
                     /locus_tag="PKV31_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92082.1"
                     /translation="MSRQHLKEYTEVGKVRKSYGLEGVLRAVLYPATVKAAVEAEYFF
                     VEEYGQKLPLFIQWMEGEGDDFHIKFEDIDDPQTAGQLATAPLFMDKRIVEELLPPDE
                     DTLPFDQKDLIGYRIKDLTSGYLIDIQDISQYPEQLMAHIVINNREEMVPLVDDLIEG
                     IDHQQRLITVRFPEGMFGL"
     gene            complement(3619..4410)
                     /locus_tag="PKV31_04160"
     CDS             complement(3619..4410)
                     /locus_tag="PKV31_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92083.1"
                     /translation="MNFNNIVLKTQPTSLLLLSLAFIAIFSACEKELDTGASEKYKEQ
                     LKESYRYYPLELNKSWIYSIDSIYYSDNLGIIEVDTVRGIYRETLVDTFRNENDQLVY
                     RCLKEKKNVNQTWSVLRVYSLTVLTNQLLRTEDNVTLVDLIFPIRSGSRWDTRVFIDS
                     DANYLIRGKAINLFKDWPDTYVANEYNGEYNGTATPMIDIIDVEADNNIILYKSSHRT
                     YARGVGMIRKEQAFFTTQRTEDPDLPWEQKAEIGFKAIQTLLEYK"
     gene            complement(4751..6037)
                     /locus_tag="PKV31_04165"
     CDS             complement(4751..6037)
                     /locus_tag="PKV31_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB92084.1"
                     /translation="MARILLIDDESSIRRTLKEILSFEKYEVVEAVDGLEGIEKVKQQ
                     KFDAILLDIKMPRMDGIEAFEKINAIAPETPVIMISGHANIDTAVDAVKKGAFDFVSK
                     PPDLNRLLITIRNAIDKNNLVVTTKTLQSKIARCKVPEMIGNSPAIRKIHETIDRVAP
                     TEARVLITGNNGTGKELVARWIHAKSNRNSAPFVEVNCAAIPSELIESELFGHEKGAF
                     TSAVKQRIGKFEQADGATLFLDEIGDMSLEAQAKVLRALQEKRITRVGGEKDICVDVR
                     VIAATNKDLLKEIEAKRFREDLYHRLAVIIVHVPSLSDRIEDIKLLSEHFINNICADY
                     GIPPKAITNDAFKVLEKYNWTGNIRELHNVIERLIILSGDKITADDVINFVQNDPHRK
                     SRKVTDIFEQFSTIDEVQKFIEVAFTDYKSGKGVKK"
     gene            complement(6256..7062)
                     /locus_tag="PKV31_04170"
     CDS             complement(6256..7062)
                     /locus_tag="PKV31_04170"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB92085.1"
                     /translation="MATYIYPTFDEPKKIIDLRGVAQSYNNGATYIIKDLNFAVTEVS
                     DNGRFVGLLGASGSGKSTLLRYIAGLQTPTMGKVLINGKEPGADNRVSVVFQEYSSMP
                     WYTVLENVALALQYKGMSKKEREEKAMEMIQLVGLDGHQHKYAQIPGLSGGQLQRVAI
                     ARSLLSNSKILLMDEPFGALDINTRLQMQDLLIKLQEQFKMYIIFVTHDISEAVYLSD
                     NIYMMKKAPSLIAEEVDIDLPAKRDRELKRDRHFIDLVYQVEDIMVNLSK"
     gene            complement(7108..7800)
                     /locus_tag="PKV31_04175"
     CDS             complement(7108..7800)
                     /locus_tag="PKV31_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNB92086.1"
                     /translation="MTVDLLKEMEKDLLVKIEKILNHPVITKIHNGELTKEQLRLFAI
                     QYSIYCGYFPRFLCACAANIPDDNTRMAIIENLWEEHGSGNLAGSHRVLFEKFAAAVG
                     ASSEDLRNAKPIPSTLINVEYLLNMCQDDSFIKSLGALGPGTEFFTSREYELIYRGLQ
                     KVEGLTESDLEFWSIHISLDDHHYSDMLNAVKTWLDSEETIDLFREGAERAIDLELLF
                     WNGLEEHLFGIS"
     gene            complement(7802..8872)
                     /locus_tag="PKV31_04180"
     CDS             complement(7802..8872)
                     /locus_tag="PKV31_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB92087.1"
                     /translation="MNLFELRGALPPKVKLILEIAGILLLLFVWFLLTLGTEPIVPAG
                     ILPSPSKVLNAIGLVFIENELVVNLCRSLGLNICGYLEAIIISVVFGFLVGLIPLFNG
                     LFAKPIDAFRFVPLTGVIGLFIVWFGLGVPMKVHFLAFGILIYMLPVIVTRINDVEDV
                     YLKTAYTLGATKWQIVRTVYIPHVMSKFSDDIRVLTAISWTYIIIIESIGNEGGLGAL
                     MWRTGIRQGNIAKLFAMLFIIIIIGFVQDKLFAMLDRRLFPFKYLKESVKASGKLTED
                     NKLRLILSIIKPYFVLLVAGIIIILLANEFIGFLGRKGILSYAFGETYPIIAVLMSVI
                     SLWIVFDFVNFRKVPKKSLEKA"
     gene            complement(8961..>9571)
                     /locus_tag="PKV31_04185"
     CDS             complement(8961..>9571)
                     /locus_tag="PKV31_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB92088.1"
                     /translation="NHGDNINYFGLNNEYKGITGEALYSKMGDKYHSLNLAPANRPNW
                     RALSNSYAVSNSTLQPTGDNAGEGNARFSPATASAKTAPAVATKTVSITFPTASYTLD
                     ENAKSLIDLQFADVAKGFGNSRVRIEGNTDNVGDKLKNVELSRKRAEAVASYLENTYN
                     MDKNRFVIIGNGMDKPVVGCESNASPECRAKNRRTEFQLIGE"
BASE COUNT         2902 a         2043 c         1815 g         2811 t
ORIGIN      
        1 aatttctgat attctcgtcg aaggttacaa attgtcttcc aaagggaagg aagtttcaaa
       61 aaggtttgca aaaaatatcg atggattgaa aatttgtttt aaaaccaacc aaaatgaact
      121 cgtggaggca gggcgtgaaa aattcttcat taggataatc aatccaacag gcgaaacagt
      181 cagtgtttta aatgcaggct caggggtaat caaggcatcc gaagatggcg ccgaaatccc
      241 gtattcgcta ctcaaggaat gtaactacga aaacgcagat gctgtagcat gcgccaactg
      301 gcaacccaat tttcagttta taaagggttc ctatgagatt catgtatata ataaagggta
      361 tatggcaggg aaaagtactt tcactctgaa gtagatgctt cagtgcgaat attgaaggac
      421 gtaaaggcat aaagattgga tccaaatgct ttgtatcttc ccgtcattgc agcccaaaat
      481 tacttcaatg attgaatgta gtgtattctt aaaagcgtga tcggttcatt tccgggataa
      541 atttatcctg ataagcaaga gacttatgga agaagaatga tcaacttctt ccctgttgcc
      601 ggaagatccc gatcaataat ccatggattt aaatacctga gcatccggta agtcgaacca
      661 ttttcgatgg caaatgctgt caggtcgggg attgccccgg ttacttctat ctgccttgtt
      721 gtagtcaatg gtttataaag ttctacctgg tcaatgtcat aaccgaaatt ggaaggacta
      781 ctcatcacct ctttaatagc cagaatacgg aagatgtatt tagaggtctc cggattgaga
      841 tacagatcat agaaattatt gactttctgt gccttcaact ctctatcaat cctaccacct
      901 ccgccattat atgcagcagc aaccagtgtc cagttgccaa atctgttgta cagggttttg
      961 ataaacttgc aagcagcccg ggtcgcaagc tctacgttgt atcgttcatc tatcttatca
     1021 ttgacgatta agcccagctc ttttgccgtg gagggcatca attgccaaaa accttttgca
     1081 cctgcagggg aagtggcatt actgaaatta ctttcgacag gaacaagata tttgaaatca
     1141 tccggaatat tctcctccat caatattctt tctatcaatg gaaaatacct tcctgccatc
     1201 ttgaggttga gcaaggtgga gccatggagg taagtattca ttaccagctc cttatcaagt
     1261 cgatccctta catcctgatt ggcaacagga acaagttctc cggcaaaatc aaaaggtcga
     1321 ttgatggatg gagatttaat agcctggggt agtggctcct gttcaaaggc atcatgaagt
     1381 gttatggatg caatcgaaat caaagcaaca cccataagat ataagaaaat ctttttaaaa
     1441 tctgttttca ttcctgagat attaaaaaat caaaccttca aagtagctaa taatcttttg
     1501 atactataac gacctgaacc attgactatt atcatcaaca agccgcccat gatagcaata
     1561 ttcttcataa acataatagc ctgaatacgc tgaaccttca ccggatcatt ccaaaaagaa
     1621 tggacaatca aagtaaccgg cagataatac aaaatcaaaa ggattgcacc aaaagatgat
     1681 ctgtaaccta tcaacaccat caaaccaccc aacacaagca atataatggc acctacgagc
     1741 aagagatctt gtctccatgt cagaccatac attgacatgg ttgcttttgt catatgaaaa
     1801 taatatatgg agtcataagc ttcataaata aaaatagtcg ctatgaaaat acggcccagc
     1861 aggtccagaa aatccttcat tgtgattgaa ttaggctaat gataaaaatc accatttcaa
     1921 ttaagcaaaa tcctctgaat cctgcctaat tacaaaaata catttttatc cttaaacagc
     1981 atccttcctc aggaaataac aatctttcac acaacaacac aaactattga atatcatgtt
     2041 tttacgaatt agctttaact gagatcaaaa aatgagcaaa ttttattaaa aatatttgta
     2101 tagcatacta tattgtattt caataatata cgctaaattg ccgctcaaat tatttaacaa
     2161 ataattaaaa tcggcataga atttgctata tattaattac tgataatctg tgattttttt
     2221 tctgaacact cacaaacgta atcgttgcaa ttatcgttaa taatattatc aacaatttaa
     2281 tccactttta gaacattttt ttatgtattt agtaaaaact ccttcatttg tcaaaagatt
     2341 tatccctttg gcattatggg gaatacggcc aggagaaaaa aaaatatttt taacatttga
     2401 tgatggaccg ataccggaag tgactccttg ggtgctggat aggctggatg agtttgatgc
     2461 caaggctact ttcttctgtg ttggagaaaa tatccacaaa catccagagg tgttcaatga
     2521 tgttattgag cgcggacacg gtgtaggcag tcacacatac aaccacttga atggttggga
     2581 aactgacaat ctggaatatc tgctgaacgt aaaaaaggca gcacaaatga gtcattcggt
     2641 cctgttcagg cctccatacg gaaagctaaa gcctaaacag gcacaatttc tgcataagca
     2701 ttatcaaatc gtgatgtggg atgtgttgag tggtgatttt gaccaaaata tctcgaaaga
     2761 gcaggtttat aaaaatgttg tcaataatgc tgttgatggt tccattattg tctttcacga
     2821 tagtttgaag gcaaaggagc gaatggagta tgcattgcct cgtgtactgg agtatttttc
     2881 cgaaaaagga ttcagttttg cggcaatcca ggaaaatgaa gcctacagtc cggcccttgt
     2941 gcctcaatat gcctgacgat tgcaacattg gacaggggca ttaagttgaa aaatacctcc
     3001 ccaggataat ctcaacaatt tctgaataat aaaaaagccg tgataggatc ccggcttttt
     3061 ttatttcaca ctgaccaaat caaaggccaa acataccttc aggaaaccgt acggtgatca
     3121 gtctttgttg atgatcaatt ccttcaatca aatcgtcgac caaaggcacc atttcttcgc
     3181 gattgttgat tacaatgtga gccatcagct gctccggata ttgagaaatg tcttggatgt
     3241 caataagata tcccgatgtc agatccttga tacgatagcc tatcagatcc ttttgatcaa
     3301 aaggaagtgt atcttcatcc ggcggcagta attcctctac aattcgctta tccatgaata
     3361 atggagctgt cgccaattga ccggctgtct gaggatcatc gatatcttca aatttgatat
     3421 gaaaatcatc accttctcct tccatccatt gaatgaagag tggcagtttc tgcccgtact
     3481 cttccacaaa gaagtactca gcttccacag cagctttgac cgttgcagga tagagtacag
     3541 cgcgcaagac tccttcaagg ccataactct ttcttacctt tcctacttcc gtatattctt
     3601 taagatgttg acgggacatt acttatattc caatagtgtc tggattgctt taaaaccgat
     3661 ctcggctttt tgttcccatg gcaaatcagg atcttcagtc cgctgtgttg taaaaaatgc
     3721 ctgttccttt ctgatcatgc ccacacccct ggcataggta cgatgtgatg atttgtacaa
     3781 gataatatta ttatccgctt ccacgtcaat aatatcaatc atcggtgtgg cagtgccatt
     3841 atattctccg ttatattcgt tggcgacata ggtatcaggc caatccttga ataaattgat
     3901 agctttgcca cgaatcagat agttggcatc ggagtcaata aatacccggg tgtcccatct
     3961 gcttcctgat cggatcggga aaataagatc aacaagggtt acattgtctt cggtcctgag
     4021 cagttgattg gttaagacgg taaggctata gacacgcaag acagaccagg tctgattgac
     4081 atttttcttt tccttgagac accgatatac caactggtcg ttttcatttc tgaacgtgtc
     4141 tactaaagtt tcacgatata taccccggac tgtatcgact tcaataatcc caagattatc
     4201 cgaataatag atcgagtcaa tgctgtatat ccagctctta ttcaattcca atggataata
     4261 tcgataggat tccttcaatt gctctttata tttttcactc gctccggtat ccaattcctt
     4321 ctcacaagcc gaaaatatgg caataaaggc caaactaagc agtaataaag atgtaggttg
     4381 ggttttcaat acaatattat taaaattcaa atgacacaaa attacgggtt cgatattcaa
     4441 atctaagtca tatccgaatc gtcagacgct tatagcttat acacaaacac agaaatcatt
     4501 tcgccacaaa agtatataac attgaagaca atgtcgaaaa ttttatagag atctaacaaa
     4561 aggctaaaaa caatagttta cgacgaaagc ccatcttctt tgaaaactct tacattatcc
     4621 tgtcctatcc aatccattgt aaatgcgata ctttcccggc ctttagccgg agaaggtagt
     4681 atgaggtcga catccggata aaggtacagt tgtcaacaag gataaaaatc tggtgtcctg
     4741 atttatcaga ttacttctta acccctttac ctgatttata atccgtaaag gccacttcaa
     4801 taaatttctg tacttcgtca atagtagaga actgctcaaa gatgtcggtt accttccttg
     4861 atttacgatg cgggtcattt tgaacgaaat taatcacatc atcggcggtt attttatcgc
     4921 cactcaggat aattagtcgc tctatcacgt tgtgcaattc ccttatgttg cctgtccaat
     4981 tgtatttttc aagtactttg aaagcgtcgt tggtaattgc tttgggcgga ataccataat
     5041 cagcgcaaat attgttgata aagtgctcac taagcaactt tatatcttct atccgatctg
     5101 aaagggatgg tacgtgtacg attattactg caagccgatg ataaaggtcc tcgcgaaatc
     5161 tctttgcttc aatttccttc aataaatcct tgttggttgc tgcaattacc cgcacatcga
     5221 cacaaatatc cttctcccct cctactcttg tgatgcgttt ttcctgaaga gctcttaaga
     5281 ccttggcctg tgcttcaagg ctcatatctc cgatttcatc caggaaaagc gtggcaccgt
     5341 cggcttgctc aaatttacca atcctttgct tcacagcaga tgtaaaggcg cccttttcat
     5401 gcccaaacag ctcactctca atcagctccg aaggaattgc agcacaatta acttcgacaa
     5461 aaggagccga atttctgtta ctcttggcat gaatccagcg cgcaacaagc tcttttccgg
     5521 ttccattgtt tccggttata agtacccttg cttctgtagg cgccacacga tctatggtct
     5581 catgaatttt tctgatggcc ggtgagttac cgatcatttc aggtacctta catcttgcta
     5641 ttttactttg tagcgtcttt gtagtcacca caagattatt tttgtcgatt gcatttcgaa
     5701 tcgtaatcaa caatctgttg aggtcgggtg gctttgatac aaagtcgaat gcgcctttct
     5761 tcacagcatc gactgccgta tcaatattgg catgtccgga aatcatgatg acgggagtct
     5821 cgggagctat tgcattgatc ttctcaaatg cctcaatacc atccatcctt ggcattttaa
     5881 tatccaaaag gatagcatcg aacttctgtt gtttgacctt ttcaatacct tccagaccgt
     5941 caactgcctc tacaacttca tatttctcaa acgacaggat ttcctttaat gtccggcgga
     6001 tactactttc atcatcgatg agtaaaattc ttgccataat ggttctattc tttttaaaaa
     6061 gggttgaata tatcaggcaa agttaaaata caaattacac attaaaaaat tatttaatat
     6121 gtaatttgta attttccaat tacacatgtt aacatttttc taatatgtca ccgaaaaagt
     6181 gataaaataa gtttacacta agtcaggtaa gactgaggct acatagggga aatgtgtatc
     6241 taagtaagta tgaatttatt tggacaaatt gaccataatg tcttccactt gatagaccaa
     6301 atcgataaaa tgcctatcgc gcttcaattc ccggtccctt ttagcaggca ggtcgatatc
     6361 cacttcttca gcgatgagtg atggtgcctt tttcatcata taaatgttat ctgaaaggta
     6421 gactgcctct gaaatatcat gggtgacaaa aatgatatac attttaaact gctcctgtaa
     6481 tttgatgagc aggtcctgca tctgaagtct ggtattgata tcaagagctc cgaatggttc
     6541 gtccatcaac aggattttgc tattggagag aaggcttctg gcgatggcaa cgcgctggag
     6601 ctgacctccg gaaagaccag gtatctgggc atatttgtgc tggtggccgt ctagacctac
     6661 gagctggatc atctccatgg ccttttcctc gcgttccttt ttggacatac ctttatattg
     6721 cagtgccagg gccacattct ccaaaacagt ataccagggc atcgaactat attcctggaa
     6781 caccacactg acgcggttat cggcaccggg ttccttacca ttgataagta ccttgcccat
     6841 ggtgggagtc tgcaggcctg caatgtatct taacaatgta gacttgccac ttcctgacgc
     6901 ccccaacagt ccaacaaatc taccattgtc agaaacttct gtaaccgcaa aattcaaatc
     6961 tttaatgata taagtcgctc cattattgta ggattgggcg acaccacgga ggtcgattat
     7021 cttcttaggt tcatcaaaag taggataaat ataagttgcc attcagtgca tataattaat
     7081 caaaatataa tcaaaatccg gaatctgtca gctgatccca aagagatgtt cttctaaacc
     7141 attccagaac aacagttcaa ggtcaattgc ccgttcggct ccctcacgga acagatcgat
     7201 cgtctcttca ctatccaacc aggttttcac ggcattcaac atatcagaat aatgatgatc
     7261 gtcaagagaa atatgaattg accagaattc caaatcggat tcagttagtc cctccacttt
     7321 ctgaagtccc cgatagatga gttcatattc ccttgatgta aaaaattcag tccccgggcc
     7381 caaagcgccc aagcttttga taaagctgtc gtcctgacac atattcagca gatattccac
     7441 attgatcaat gtggatggaa taggtttggc attccgcaga tcctcagaag acgctccgac
     7501 agcggcggcg aatttctcga acaaaacacg atgtgatccg gcaagattac ctgagccatg
     7561 ttcttcccat agattttcaa tgatagccat ccgagtatta tcatcaggta tatttgcagc
     7621 acaagcacac aggaatcttg ggaagtatcc gcaatatata ctgtattgaa tggcaaacaa
     7681 ccgaagctgc tctttagtca attcgccatt atggatcttc gtaatcacag gatgattcag
     7741 aatcttttca attttaacca aaagatcctt ctccatctct ttaagtaagt caacagtcat
     7801 atcaagcttt ttccaggctc tttttgggaa ccttcctaaa atttacaaaa tcaaagacaa
     7861 tccaaaggga aataacagac atcaatacgg caattatcgg gtaagtctca ccgaatgcat
     7921 atgacaatat accttttcta cccagaaagc cgatgaattc attagccagt aagataataa
     7981 taattcctgc caccaaaagc acaaaataag gcttgatgat cgacagtatt aatcgcagtt
     8041 tgttatcttc cgtgagcttt cccgaggcct taactgactc tttcagatat ttgaaaggga
     8101 aaagtcgtcg gtccaacata gcaaataatt tgtcctgaac aaaacctatg atgataataa
     8161 tgaagagcat ggcaaacaat ttggctatat tgccttgtct gattccggta cgccacatca
     8221 aggcgccaag cccaccctcg ttgccaatac tttcaatgat gatgatatat gtccaggaaa
     8281 tggctgtcaa cactctgata tcatctgaaa atttcgacat tacgtgtgga atatagactg
     8341 tacgtacaat ttgccatttg gttgccccga gtgtataggc tgttttcaaa taaacatctt
     8401 cgacgtcatt gattctggtt acgatgacag gcagcatgta tatcaggata ccaaatgcca
     8461 ggaaatgtac tttcatgggt acgcctaacc cgaaccaaac aataaaaagt cctataacac
     8521 ctgtcaaagg gacgaaacga aaagcatcaa taggtttggc aaaaagtccg ttgaacagag
     8581 gaataaggcc taccagaaat ccaaagacta cagatatgat gattgcttcc agatatccgc
     8641 aaatatttaa tcccaatgac ctgcataagt tgacgaccaa ttcattctcg ataaacacca
     8701 ggcctatcgc gtttaatacc tttgaaggag aagggaggat accggcaggt acaattggtt
     8761 ccgtacccaa agtcagcaaa aaccacacaa acagcaacag tagaataccg gcgatttcca
     8821 ggatcaactt tactttgggg gggagagcgc ctcgcaattc aaaaagattc atcaaaagtg
     8881 tattttattg accacaataa gggattgaat gcaagaaaaa aggcaggagg atcaactcct
     8941 gccttttgac tatagatctt ttactctccg attagttgaa actcagtgcg tctgttcttt
     9001 gcacggcatt caggagaggc attactctca caacctacaa caggcttgtc catcccgtta
     9061 cctatgatga caaagcggtt tttatccata ttataggtat tttcaagata gctggcaaca
     9121 gcctctgcac gtttacggct caattctaca tttttcagtt tatcaccgac attgtccgta
     9181 tttccttcta tcctgactct tgagttaccg aaaccttttg ccacatctgc aaattgcagg
     9241 tcaatcaaag atttggcatt ttcgtcaagg gtataactgg ccgtaggaaa ggtgatgctt
     9301 acagttttgg tagccactgc aggagcggtt ttggcggaag ctgttgcggg agaaaatctg
     9361 gcatttcctt ctccggcatt gtcaccggtt ggctgaagag tagagttaga cacagcgtag
     9421 ctgtttgaca atgctctcca attaggtcgg tttgcagggg cgagattcag tgaatgatat
     9481 ttatcaccca ttttggaata gagtgcttcg cctgtgatgc ccttatactc attgttgagg
     9541 ccaaaataat tgatattgtc tccatggttg g
//
LOCUS       DAOQMC010000069         9550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2792_length_9550_cov_5.006214, whole genome shotgun sequence.
ACCESSION   DAOQMC010000069 DAOQMC010000000
VERSION     DAOQMC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_9550_cov_5.006214"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1752
                     /locus_tag="PKV31_04190"
     CDS             22..1752
                     /locus_tag="PKV31_04190"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB92089.1"
                     /translation="MIDSNPLLSIRDLRISFETEGRVNSVIKGINLDIGKGEIVGLVG
                     ESGSGKSVTGLSLCGLLPENNARITGSIQFDHFGNAKELVGMTDNAFRQLRGSNISMI
                     FQEPMSSLNPVLTCGFQVDEMTRLHLKYNKEQARNTTLELFDKVGLPDPHRIYRSYPH
                     QISGGQIQRVMIAMAISCHPQLLIADEPTTALDVTIQKKILELLVKIRKDFGISILFI
                     SHDLGVIKDITSNINVMYRGRIVEAGSTESVLDNPQHPYTRGLIACKPPLRYKKERLP
                     VVSDFVTIEETSDDYVFKPVVKSEDRPIPKKDPVCFDQAPLLEVDNLVVKYPIASNLW
                     GKTTSWLRAVDDMSFSVYRGETLGIVGESGCGKSTLGRTIVHLEKASSGVVFFEGRDL
                     LKQRESDWKSTARQVQIIFQDPYSSLNPKMNIGAAIMEPMMVHNLYNSHKGRREKMLT
                     LLEQVGMLPEHADRYPHEFSGGQRQRISIARALGLNPQLLICDESVAALDVSVQSQVL
                     NLLKDLQAEYGLSMLFITHDMSVVNFIADRILVMHKGKVVEAGSVEEVIRNPKEDYTM
                     RLIESIPQWS"
     gene            1868..2239
                     /gene="rbfA"
                     /locus_tag="PKV31_04195"
     CDS             1868..2239
                     /gene="rbfA"
                     /locus_tag="PKV31_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNB92090.1"
                     /translation="MSIRKLQVAELLRRNLSHIFQADGRYIYGTEPMVTVTSVQVTPD
                     LAQAKVYLSIFNTDNKQAVLLEIEAAIVEIKRNLYNRIRKHVRRVPDVIFFVDDTLDE
                     MVKVDEMMRKLRADNQMGEEE"
     gene            complement(2246..2878)
                     /locus_tag="PKV31_04200"
     CDS             complement(2246..2878)
                     /locus_tag="PKV31_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92091.1"
                     /translation="MKNYILLIIFIHFSIFALSQESSRWQVMPALIGGVFPNYEYSLK
                     NYNPLLIPYLPFSKGGFYGELRYNYDRNGTFGIYGGKSWSLGENTIHLITPQLGAIFG
                     DYQGISLQLYYNLINPHVEFNLTNNYALIFNNRPNFYFNWSDIQFPVWKKGRIGASTQ
                     IFIDSAVKTYDFGPMIGYRNEKWYFMLTSFNPWQQEKHYLFFAIQRTFQL"
     gene            complement(2962..3252)
                     /locus_tag="PKV31_04205"
     CDS             complement(2962..3252)
                     /locus_tag="PKV31_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HNB92092.1"
                     /translation="MRSIMMVGIGSAPGSLSRYGIVLMGKIFQWQLPGIPTFIANIIG
                     CFLFGILITLALKENIIIEEIKLFLSVGFCGGLTTFSTFTAENFTYLNQKII"
     gene            complement(3311..4177)
                     /locus_tag="PKV31_04210"
     CDS             complement(3311..4177)
                     /locus_tag="PKV31_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB92093.1"
                     /translation="MMGRLHSLNYRKSSCIYLVRQYSILKVIRTTNRTFHVLLYSIGF
                     LFFSYKSNGQSEQKMNVYFISGLGADQRVFQKLTIDPEFNPVPIEWVKPGRKESMPSY
                     ARRLSTQIDTTKPFQIVGLSFGGMIAAEIADQIHPSQIILISSKSTSEPIGKFYRGLI
                     HLGLLSPFASLLLRSANRFTYKAFGAKTTEEKTMLRSILKDTDPKFMKWAIKTISSWS
                     HPKKSPIAYQINGSADRMIKANMVQYDYLVLGGEHLMIYSMASQISKILNERLKYGLH
                     QSMSKSISNELK"
     gene            complement(4342..5082)
                     /locus_tag="PKV31_04215"
     CDS             complement(4342..5082)
                     /locus_tag="PKV31_04215"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNB92094.1"
                     /translation="MNFNKLILLTFLTLCNIHSVISQNDFFSKAKKAAKEITSGNTTS
                     LSNDEVIAGLREALTIGAQNSAGLASKVDGFYKNPAIFIPWPSEARNMRDKLIALGMS
                     KQIEEFERSLNRAAEEASKSAIPIFTDAIKKMSLNDGFNILKGADTAATHYLRVNTTD
                     GLTEAFSPVVANAIDKVQVTSYWTPLVNAYNKIPFVKKQDPDLKMYVTHKAIDGLMKL
                     LADEETKIRTNTSARVTDLLKKVFGNNN"
     gene            complement(5184..6455)
                     /locus_tag="PKV31_04220"
     CDS             complement(5184..6455)
                     /locus_tag="PKV31_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92095.1"
                     /translation="MKIINRSMLLIFALMVGVISINGQDIQKDSTGLPGDNFNLQGTL
                     DLFSKANDLESFEKSINDEKNNVNNLDLNGDGQIDYIKVIDRTDGNAHAIVLQAVLGN
                     NDNQDIAVIEIEKKGNEDAVAQIVGDEDIYGKEVYVEANSDNSSIDDDGNQIRKGPAA
                     YSPNTFRHIYVNVWFWPCVQYIYSPRYIVYVSPWHWHSYPMWWRPWRPWHWHTFYHGG
                     WHYHHHYVVVSHPRVYGARKVYAPHRVVSNNVEVRNKTVINNYRIKNNIVIDKNDSRH
                     RGSNNNDNPAIRENMNSRTRQSDPGNMQNGSGATKSDRNTTDQPRRDNINRNNSSDRP
                     TRVDRPTENGTLSPSRDQQPAKREERYTPMKNTEKSMPDKQSNQPSHRQPAPQRMQRD
                     AQPGKPMNSKMERQRSRSSDSRQGIPSSRRG"
     gene            6680..8281
                     /locus_tag="PKV31_04225"
     CDS             6680..8281
                     /locus_tag="PKV31_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92096.1"
                     /translation="MNWKFNYRLLSRITAAILLGIVLIRFFLINIVDTALRNQLEKIR
                     QPDLSIDYNGLSIGWYSPSVYIDHMEVVGMGQSDTAMKISVERIHFQDIRLRKLFFHK
                     EIIVGRISLDQTVLSTKTGQRMEWKNFPKQGNLNSGLKLFSKEFRFKNFQWNHFDERD
                     ELAMIAGTQRFSIFGIQYRSKALPGDGVLFDSIGCRDFSFVSPAIQHKITIGSVDYSR
                     NLKELNIGEVRVVPLLDKFEFAQYFKRQVDRIECHSREIKLTDVTYKDKGSMYLKAGK
                     MAFDFHMETYRNKTYPFNRKKASPMPRELLKKTGFIFDIDTFELHDGFIAYEELDSLY
                     NNPGRIYFKGLNIRANHFSNDSKYNGNTLRATSQFMESGVIEAEFFFPEDSAKKYRVQ
                     GSLSPMDLSEINGILKAASLFEVKTGTLDTMRFLFDYDRVEASGKVEFAFNDLKLVAY
                     KKKSPEKISIMKTLVINQVIRMNKVKNANLIGHIGFKRDPVRSVVSYWWRSLFSGIKS
                     AFVKDGSFNPTRNESGTLGPKFKDE"
     gene            complement(8294..>9550)
                     /locus_tag="PKV31_04230"
     CDS             complement(8294..>9550)
                     /locus_tag="PKV31_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92097.1"
                     /translation="NKIRYRFAQHSDRSATLTIPLKQPKSTLIKILMEENSRLTDSLT
                     YDITAWNLPKAFGLNYSITANPMDIKNPPKTIKPTATAYGYAIAWQGGNVSKALSWML
                     QQGIRLRFSENEFSLEGTKFDRGTLLILDSGSNRDKNIREVMDSVVNKFDIAIKPISR
                     GMVDTGNDMGSESIRVINAPKVILLSGEGVFSLSLGEIWFHMDMELDFPVSLVNTSQL
                     RNFNWSDADIIILPDGNYSFLRDKELTDQLENWIRRGGKLIAFENAVNQLTGLNWVHL
                     KKKDSPADSTSANKYSSLKKFGDRERAELKNYIPGSIYRIDLDNTHPLAFGYPSYYYS
                     LKLNSTFYNYIKDGGWNVGVIKGGTEKSGFIGSNVRSEFKDAFIFGVEQVGKGNIVYF
                     TDNILFRNFWENGKLLFDNALFLVGQ"
BASE COUNT         2703 a         1800 c         2036 g         3011 t
ORIGIN      
        1 aatattacag acaaataaaa aatgattgac tcaaatcctt tacttagtat ccgcgatctc
       61 aggatttcct tcgagactga aggcagggtc aactctgtga ttaaggggat aaatctggat
      121 attggtaagg gtgaaatcgt tggacttgtt ggagaatccg gaagtggaaa atccgttaca
      181 ggattatccc tctgcggtct tttaccggag aacaatgccc ggattaccgg ttcaattcaa
      241 ttcgatcatt tcggcaatgc taaggagctg gttggcatga ccgataacgc gtttcgacaa
      301 ctccgtggaa gtaatatttc catgattttt caggagccca tgtcatctct gaatcctgta
      361 cttacttgtg gatttcaggt agatgaaatg acccgactcc atctgaagta caataaggaa
      421 caggcgcgaa atacgacgct tgagttattt gacaaagttg gcctccctga ccctcatagg
      481 atttatcgtt cttatccgca ccagatttcc ggtggacaga tccaaagagt gatgatagct
      541 atggccatta gttgtcatcc acaattgttg attgctgatg aacccacaac tgcattggat
      601 gtgacaatcc agaaaaagat acttgaattg ctggtgaaaa tccgtaaaga ttttgggatc
      661 tctatccttt ttatttcaca tgatcttggt gtaatcaagg atattacctc aaatatcaat
      721 gtgatgtacc gagggaggat agtagaggcg ggttctacag aatctgttct tgataatccc
      781 caacatcctt ataccagagg tctgatagcc tgcaagccgc cattgagata taagaaagaa
      841 cggttgccgg ttgtaagtga ctttgtgact atagaagaga cgtcagatga ttatgttttc
      901 aagcctgtag ttaaatcaga ggatagaccc ataccaaaga aagacccggt atgtttcgat
      961 caggcacctc tcttggaggt ggataatctt gtcgtaaaat atcctatagc ttcgaatttg
     1021 tggggtaaga cgacttcgtg gctcagagct gttgatgaca tgagtttttc tgtttataga
     1081 ggagagaccc tcggcatcgt gggcgagtcc ggctgtggca agtcaactct ggggagaacc
     1141 attgtccatc tggaaaaggc ttcatcaggg gttgtctttt ttgaaggcag ggatttactg
     1201 aagcaacggg agtctgattg gaagtccaca gcacgccaag tccagattat attccaggat
     1261 ccatactctt cactcaatcc aaagatgaat atcggagctg ccattatgga gccgatgatg
     1321 gtacataatc tgtacaatag tcataaaggc aggcgtgaaa agatgttgac attacttgaa
     1381 caagtgggta tgctaccgga gcatgctgat cgttaccctc atgaattcag cggcgggcaa
     1441 cgtcagcgta ttagcatcgc cagagcgttg gggcttaacc cacagttgtt gatctgtgat
     1501 gaatctgtgg cagccctgga tgtaagcgta cagtctcagg tgctcaacct tttaaaggat
     1561 cttcaggcag aatatgggct ttccatgctg ttcattactc atgatatgtc ggttgtcaac
     1621 ttcattgctg ataggatttt ggtgatgcat aaaggtaagg ttgtggaggc aggttcagta
     1681 gaggaagtca tccgtaaccc aaaagaagat tatacgatgc gacttattga gtctattccg
     1741 caatggagtt gaaaagtcat tgatcgtgat tggatggggt caccaggtat tgacttgctt
     1801 tcgacccgga tattcatata tttcattacc tttgtacagc tacgaatatc gaaataatta
     1861 tcagaccatg tccatccgta aattacaagt tgccgaattg cttcgcagaa acctaagcca
     1921 catttttcag gctgacggta gatatatcta tggcacagag cctatggtta cggtaacgtc
     1981 ggttcaagtg acgcctgatc ttgctcaggc caaggtatat ttaagtatct ttaatactga
     2041 taacaaacag gccgtactat tggaaattga ggcagcaatc gttgagataa agcgtaatct
     2101 ttacaaccgg atcagaaaac acgttcgacg cgtaccggac gttattttct ttgtcgacga
     2161 taccttggac gaaatggtta aggtggatga aatgatgcgc aagttgaggg ctgacaatca
     2221 gatgggcgaa gaagaataac ccggatcata gctgaaaggt cctctggatt gcgaaaaata
     2281 aataatgctt ctcctgctgc catggattga atgaagtcag catgaaatac catttttcat
     2341 tcctgtatcc gatcatgggg ccaaaatcat atgtcttaac ggccgaatct atgaaaattt
     2401 gagtggaagc tccgattcta cctttcttcc atacagggaa ttgaatgtca gaccagttaa
     2461 aataaaaatt ggggcgattg ttgaaaatca gtgcgtaatt attagtcagg ttgaattcga
     2521 catgtggatt gatgaggtta taatacaatt gcaatgaaat tccctgatag tcaccaaaga
     2581 tggcgccaag ttgaggggtg atcaggtgga tggtattttc cccgagtgac caagattttc
     2641 ctccataaat accaaaagtc ccatttctgt cataattgta ccgcaattct ccataaaaac
     2701 ctccctttga aaatggcaaa tacggaatca atagtggatt gtaatttttg aggctgtatt
     2761 cataattggg aaagacaccg ccgatcaaag caggcattac ttgccatctg cttgattcct
     2821 gacttagggc aaatatggaa aaatgaataa agatgataag taatatgtaa ttcttcatat
     2881 tctaattttt aatacaaatt gagccacgcc aaggagcaac caaactgaaa aataaacttg
     2941 ctgcggtata caaggcaaat attatattat tttttggttt aggtatgtaa agttttctgc
     3001 ggtaaatgtc gagaaggtgg tcagacctcc acagaatcca accgaaagga agagctttat
     3061 ttcttcaatt attatattct cctttagtgc cagcgtaatc aggatgccga atagaaaaca
     3121 accaattata tttgctataa aggtgggtat tccaggcagt tgccattgga atatctttcc
     3181 cattagtacg attccatatc ttgaaagact tccgggtgca ctgcctattc caaccatcat
     3241 tattgatctc atggagtaat attaagaaaa aattcatatt gccaagtcaa aattaaatta
     3301 gcggataatt ttatttcaat tcattagata ttgacttgga cattgattga tgcaatccat
     3361 atttcagtct ctcattgaga attttagata tttggcttgc cattgaataa atcatcaggt
     3421 gttctccacc aaggacgaga taatcatatt ggaccatgtt ggctttgatc atcctgtcgg
     3481 cacttccatt aatctggtat gcgataggtg attttttagg atggctccaa gaagaaatcg
     3541 ttttaattgc ccatttcatg aacttaggat ccgtatcttt cagaatagag cgcagcatgg
     3601 tcttttcctc tgttgttttt gctccaaagg ccttatatgt gaatctattg gctgatctca
     3661 gaagtaaaga ggcaaatggg ctgagcaggc ccaggtggat aagtccgcga tagaattttc
     3721 ctatgggctc actggtactt ttgctagata taaggatgat ttgactggga tgaatttggt
     3781 cggcaatttc agctgctatc attcctccaa aggaaaggcc tacgatctgg aatggcttgg
     3841 ttgtgtcaat ctgagtactt aaccttcgcg catatgaagg catcgattcc ttgcgtccag
     3901 gcttgaccca ttcaattggg accggattaa actcagggtc aatagtcagt ttttggaaca
     3961 ccctttgatc ggcgccaaga ccactgatga aatagacatt cattttttgt tcagattgtc
     4021 cattggattt gtaggaaaag aacaagaaac caatagaata aagcaaaaca tgaaatgtcc
     4081 gattggttgt tcttataacc ttcagtatgg agtattgccg gacaagataa atacagcttg
     4141 attttctgta atttaaacta tgtaatctgc ccatcatatg tgagttttca caatattacg
     4201 catacaatgg aaaaaattat caaagttttg agtttcttcc aaatatttgt tgatgaagat
     4261 taatttttca aaaatattta attcgtcctt tatttttctt ccagagttgt gtggataaga
     4321 tatttcgatg gattgttccg cttaattatt atttccaaag acttttttga gcaagtcggt
     4381 tacccgggcg gatgtattag tcctgatctt ggtttcttcg tcggccagaa gcttcattag
     4441 accgtcgata gccttgtgtg ttacatacat tttcaaatca ggatcctgct tttttacaaa
     4501 cggtatttta ttgtaggcat tgaccaatgg agtccaatag gatgttacct gtactttgtc
     4561 gattgcgttg gcgacgactg gactaaaggc ttcagtaagg ccatcagtcg tatttaccct
     4621 taagtagtgg gtggcagctg tatcagctcc tttcagaata ttgaatccat cattgagact
     4681 cattttttta attgcatccg taaatattgg gatcgcgctt ttggatgctt cctcagcagc
     4741 tctgttaagt gatctctcaa attcctctat ttgtttgctc atacctaagg ctattagttt
     4801 atctctcata tttcttgcct cggaaggcca ggggataaat atggcaggat ttttataaaa
     4861 gccatcgact ttagaggcta atccggcaga attttgagcc cctattgtca gtgcttctct
     4921 aagacctgca attacctcgt cattagacag ggaagttgtg ttaccggacg taatttcctt
     4981 ggcagctttt tttgccttac tgaaaaaatc attttgtgaa attactgaat ggatattgca
     5041 cagggtgaga aatgttaata gtattagctt attaaaattc attctggtat attttgctat
     5101 ctaagatcaa taaatgatgg gaacggtctc attattaaaa ccgttccctt gtattcaata
     5161 attatttaaa ccttttgttt catttagcca cgcctggatg acggtattcc ttgtctcgag
     5221 tcagaggacc ttgatcgttg tcgttccatt ttgctgttca ttggttttcc cggttgtgca
     5281 tctctttgca ttctttgagg tgcaggttgc ctgtgtgagg gttgattaga ttgcttgtcc
     5341 ggcatacttt tttctgtatt tttcattggg gtataccttt cttccctttt tgcaggttgc
     5401 tgatcccttg aaggtgacaa ggtgccgttt tcagtcggac gatccactct ggttggtcga
     5461 tcggagctgt tgttccgatt gatattgtcc cttcgtggtt gatccgttgt atttctatca
     5521 gattttgttg caccagaccc gttttgcata ttgccggggt cggattgcct ggttcgacta
     5581 ttcatattct cccggattgc cggattatca ttgttgttgc ttccgcgatg gcggctatcg
     5641 tttttatcaa taactatatt atttttgatt ctgtagttat tgattaccgt tttatttcta
     5701 acctctacat tatttgatac aaccctatgg ggggcataga ccttacgtgc accataaact
     5761 ctggggtgac taacgacaac gtaatgatgg tgataatgcc accctccatg gtagaatgta
     5821 tgccaatgcc atggacgcca tggacgccac cacattgggt aggagtgcca gtgccagggt
     5881 gaaacataga caatatatct cggtgaatag atgtattgga cacaaggcca aaaccatacg
     5941 ttgacatata tgtgccggaa tgtattcggg ctgtatgctg ctggaccctt cctgatttgg
     6001 ttcccgtcat catcgatact tgaattgtct gaatttgcct caacataaac ttcctttccg
     6061 taaatatctt catctcctac tatttgagcg actgcatctt catttccttt tttttcgatt
     6121 tcaataacgg caatatcctg attgtcattg tttccaagca ctgcttgtaa tactatagca
     6181 tgtgcattcc catcagttct atcgattact ttgatataat caatttgtcc atcaccgttc
     6241 aggtcgaggt tatttacatt gtttttttca tcgttgatgg atttttcgaa tgattcaagg
     6301 tcattggctt tactaaacaa atccagggtc ccctgaaggt tgaaattatc acccggaaga
     6361 cctgttgaat ccttttggat atcctggcca ttgattgaaa tgacacctac catcaaggca
     6421 aaaatcaaaa gcattgatct atttattatt ttcatggcat aaattggttt atgattgaat
     6481 aattgacttt ttttattact ttgatactta aaaaacgtat tggtttatcg gaaagtaacc
     6541 aggttttcat taaagttgag ttaaagattt ttcaataatg ttaaaaaaaa tcaaagaata
     6601 aatatacagg ctctttaaat gttaaacctt tattttaagc cgatatgcac caagatgaaa
     6661 agcttatctt tacctttaaa tgaattggaa gttcaattat cgattactca gcaggatcac
     6721 tgcagccatt ctcttaggaa ttgtcctcat taggtttttt ctaatcaata tcgttgatac
     6781 ggcactccga aatcaactgg aaaaaatcag acaacctgat ttgtccattg attataatgg
     6841 actgtctatc gggtggtaca gtccatcggt atatattgat cacatggaag ttgtagggat
     6901 ggggcagtcc gatactgcaa tgaaaatatc tgttgaacgc atacacttcc aggatatcag
     6961 actccgtaaa ttgttttttc acaaggaaat tattgttggc cgtatttcac tggatcaaac
     7021 agttctttct accaaaacgg gccaacgcat ggaatggaag aatttcccga aacaaggaaa
     7081 tttgaattcc gggttgaaat tgttcagtaa ggaatttagg tttaaaaatt ttcaatggaa
     7141 tcattttgac gagagggatg aattggccat gattgcaggg actcaaaggt tttcaatatt
     7201 tgggattcaa tacagatcaa aggcgcttcc gggcgatgga gttctttttg atagtatcgg
     7261 ttgtagggat ttttcttttg tatccccggc aattcaacat aagattacga ttggcagtgt
     7321 tgattattca agaaatttaa aagaactgaa tattggggaa gtaagggtgg ttcctttatt
     7381 ggataaattt gagtttgcac aatactttaa aaggcaggtt gaccgaattg agtgtcattc
     7441 cagggaaatt aaacttacgg acgtaaccta taaagacaag ggtagcatgt atcttaaagc
     7501 gggaaagatg gcttttgatt ttcatatgga aacctacaga aataagacct acccctttaa
     7561 cagaaaaaag gcaagcccaa tgccaaggga attgctgaag aagacgggtt ttatattcga
     7621 tattgacacc tttgagctac atgacggttt tattgcctat gaagaattgg attccttgta
     7681 taataatccg ggaagaatat actttaaggg attgaatatt agggcaaatc acttttccaa
     7741 tgattccaaa tataatggaa atacactccg ggcaacaagc caatttatgg aatcaggtgt
     7801 cattgaagct gagtttttct ttccggaaga ttccgcgaag aagtacaggg tgcaagggtc
     7861 attgtcaccc atggatttga gtgaaatcaa cggaatcttg aaggctgcat ctctttttga
     7921 agtaaaaaca ggtacactgg atacgatgag atttcttttt gattacgata gggtggaagc
     7981 atcaggaaag gtggaatttg ccttcaatga cttgaagctt gtggcataca aaaagaagtc
     8041 tccggaaaag atttccatca tgaagacttt ggtaatcaat caggtcatca ggatgaataa
     8101 ggttaaaaat gctaatctga ttgggcatat tggatttaag agggatccgg taagatccgt
     8161 agtgagctac tggtggcgct cattattttc tggaattaag tctgcctttg tcaaagatgg
     8221 gtcatttaat ccaaccagga atgagtcggg gaccttaggg ccaaaattta aagacgaatg
     8281 agctgtctct tgcctactga cctaccagaa acagcgcatt gtcaaacaac agtttcccgt
     8341 tctcccaaaa attcctgaat agaatattgt ccgtaaaata aacgatattg cctttcccaa
     8401 cctgctcaac gccaaatata aaagcgtcct taaactctga ccggacattg gatcctataa
     8461 atccagactt ttctgttcct ccttttatga cgccgacatt ccatccgcca tccttgatat
     8521 aattatagaa tgtactgttg agtttgagtg agtagtaata ggatggatac ccgaaggcta
     8581 gtggatgggt gttgtccaga tctatcctgt atatcgatcc gggaatataa ttcttcaatt
     8641 cagcccgctc tctgtctcca aattttttca aagaagaata cttgttggca cttgtggaat
     8701 cagccgggct atccttcttt ttaaggtgta cccaatttaa tccggtgagt tggttgactg
     8761 cattttcaaa tgcaattaat tttcctcctc gccggatcca attttccaac tgatccgtta
     8821 attccttgtc tctcagaaaa gaataattcc catcaggcaa aatgataata tcggcatcgg
     8881 accaattaaa atttcgcagt tgagatgtat tgaccaggct tacaggaaag tctaactcca
     8941 tatccatatg gaaccaaatt tcacccaacg acaaggaaaa gacgccttcg cctgacagca
     9001 ggatgacttt cggggcattg atgactctaa tggattcgct acccatatca ttccctgtat
     9061 ctaccattcc acgggatatt ggttttatag caatatcaaa cttattcact accgaatcca
     9121 tcacttcacg gatatttttg tcgcgattgg atccgctatc taggatcaaa agagtgcctc
     9181 tgtcaaattt tgttccctcc agactaaatt cattttctga gaatcttaaa cggattccct
     9241 gttgaagcat ccatgacaag gctttactta cattgcctcc ctgccaggcg attgcatagc
     9301 cataagcagt ggctgtgggc ttgattgttt tgggtggatt tttaatgtcc atcggatttg
     9361 ctgtgattga gtaattgagg ccaaatgcct ttgggagatt ccaggctgta atgtcataag
     9421 ttaaggaatc ggtcagcctg gagttttctt ccataagaat cttgattaat gtcgatttgg
     9481 gttgtttaag gggaatggtt aaagtagctg atctgtctga atgttgagca aatcgatatc
     9541 gaattttgtt
//
LOCUS       DAOQMC010000070         9542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2794_length_9542_cov_5.075366, whole genome shotgun sequence.
ACCESSION   DAOQMC010000070 DAOQMC010000000
VERSION     DAOQMC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2794_length_9542_cov_5.075366"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PKV31_04235"
     CDS             complement(<1..507)
                     /locus_tag="PKV31_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNB92098.1"
                     /translation="MTREDFSHKASEWAAAGQPFVFLMDFEMEKPVICKPEDAATHGL
                     HFDIAGISNTDNIPISAIPDLKSSPVSREWYSERFRLVKEHIRHGDSYLLNLTFPTPI
                     DCNLSLSDIYHTARAPYRLLYRDEFVVFSPECFVRIKDDEIFTYPMKGTIDARLEDAE
                     NKLLDNLKE"
     gene            complement(504..1079)
                     /locus_tag="PKV31_04240"
     CDS             complement(504..1079)
                     /locus_tag="PKV31_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNB92099.1"
                     /translation="MRILLIDNNDSFTYNIVELLRSLDDVLYGVLPSKGLNIAVLEDY
                     SHIVISPGPMTPSDFPELRDVISYCEKLDKPLLGICLGHQAICEYFGGRLVQMDTIVH
                     GHRERIAIDNRSSIYRYLPDRIEVGLYHSWKIDHLNLPDELAVTGMSQEDCLMSVQHN
                     KKQIFGIQFHPESFLTAKGRQILENFVNIGK"
     gene            1564..2568
                     /locus_tag="PKV31_04245"
     CDS             1564..2568
                     /locus_tag="PKV31_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNB92100.1"
                     /translation="MRKIFIVILILCITGVVFSQSKQNTMTKADKNNPLLCNPQSGTC
                     EVQDSMVADSVRINISPSVTDKIRVVYFTDPICSSCWGIEPQLKKLKLEYGEVIDVSY
                     HMGGLLPDWSYNSGGISKPSDVASHWDEVSLHYDMPIDGDVWLEDPLSSSYPPSIAFK
                     AAQLQDKDKALDFLRIIREMVFLEKKNITRLQYMSEAASMAGLDTVRFKNDIEGRAVS
                     LFEEDLQLARTYGVRGFPTIFLEGKGGAREMVYSARPYAFYEVAVLKLAPNVVKQEYS
                     KDWQFLFSKYSSLTVKEFAELSGMRRSECEAFLNNLVADGHLTRLTTKNGSIWRRTAK
                     "
     gene            complement(2647..5103)
                     /locus_tag="PKV31_04250"
     CDS             complement(2647..5103)
                     /locus_tag="PKV31_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92101.1"
                     /translation="MKNALLLLIFCSVNTFIYAQNRTISGTIMDADTKGPIEFCNVFQ
                     SKDQFATISDENGRFNLLISASVHKSFLIFHSIGYRPDSIPIRPDKDNYQIFLHAENL
                     LLEDIVITGATRATLIKENPVSITAISRKQIEKATESNIIDALVKNVPGLNAVKTGPN
                     ISKPFIHGLGYNRVLTIYDGMRQEGQQYGDEHGLEVDHYNIDKAEVIKGPAGLLYGSD
                     AIAGVISLFPVIPTGNEGTFYGKYLSEYQTNNNLIGNGVTLNFSRERLLFALRGSYRT
                     AKNYRNPTDGRVYLTNFNESNFSAFAGFKSKKGYTHLNFTLYDNNQGIPDGSRDSLTR
                     KFTKQIFEGEYDDLKTRPIVSDKELNTYKVPDLAQHIQHYRIYVQGFYQIGKGEVDYL
                     LGGQQNTRREYTHPSMPQQAGMYMRLNTLNYGIRYNAPKFANIETSLGVNGMLQNNKN
                     KDATDFPIPDYHLYDGGMYLYARIKKGKWSVSGGVRYDLRLVQWNDFYVGINPETGFE
                     HQTHANDSKANLQFPEYQNSFHGISGSIGTTYKISPNINLKANIGRAYRSPNITEIGS
                     NGLDPGAHIIYLGNRNFKPEFSLQEDFSISLRYKNISAEAGFFNNNIQNYIYMATVAD
                     AQGFPITDAQGNRTYQYQQSSAHLFGSELWFAVHPERLVGFRWDNSLSIVYGLNKNPE
                     FKGKGNEGEFLPLMPPLKVTSRIEYEVKPHTGRLLSITPGFEMEYNHAQNRYLALNGS
                     ESFTPEYTLFNLGLNAEVKYNNSKAIQIILQLNNMLDKAYRSHLNRLKYFEYYSSSPN
                     GHDGIYDMGRNLSLKIIVPL"
     gene            5192..5848
                     /locus_tag="PKV31_04255"
     CDS             5192..5848
                     /locus_tag="PKV31_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNB92102.1"
                     /translation="MVSQTEENYLKALFHLTYETENKSEAGTNELATYLEVKPATAND
                     MLKKLKDKGLVSYEKYGKITLTNKGSNIAIEIVRKHRLWETFLYEKMNFTWDEVHEVA
                     EQLEHIKSQKLVEQLERFLDFPEIDPHGDPIPNARGEVRKIVRKSLSEVCIGDVCKLV
                     AVKDGSVAFLQYVAKLGLGLSTKIKVVSRQEFDGSMEIEVNNQHFTVSQKFANNLYVV
                     "
     gene            complement(6033..7028)
                     /gene="tsaD"
                     /locus_tag="PKV31_04260"
     CDS             complement(6033..7028)
                     /gene="tsaD"
                     /locus_tag="PKV31_04260"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNB92103.1"
                     /translation="MPVTILAIESSCDDTSAAVVRDGKVLSNVVSSQDQHALWGGVIP
                     EVASRAHLRLIMPAVNKALKDASVDLKDIDAIGITRSPGLLGSLHVGVTFAKSLALAL
                     NIPLIDVHHMDAHIMANFIEAPVPSFPFLCLTVSGGHTQISIVRDYLDVEVIGATIDD
                     AAGEAFDKTGKILGLDYPAGPIIDKLAAQGNPVFSFPEPNIPGLDFSFSGLKTSILYF
                     LQKATTDNPAFIRENITDLCASVQSRIVSILISKLQKAVHQTGIREVALAGGVAANSG
                     LRKAMETMHEQEGWNIYIPRIAFCTDNAAMIGQNAYYKYLAGIFAGQDMEASARG"
     gene            7092..7955
                     /locus_tag="PKV31_04265"
     CDS             7092..7955
                     /locus_tag="PKV31_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNB92104.1"
                     /translation="MSSEPYLIIVPQLITEYKHPVSGVISTYGSNLLLLSENNYLAYC
                     IVAEDGRYELLREYRIATGEKFTDFHRAVLATDEYLGNAFRSVSIGISNKRMVLVPGA
                     VFNREESVRYLESGAPVWSTDEVKADYLEKQDIHLVYAFRSAIYQAYRNAFPNMKLEN
                     ALSTYIKRLEAIVALHDLGYKVFVNTLHDTMYIVVYRNRQLCYANVYEFATAEDFLYY
                     IMLIYKEFQLDPESVPTYLSGKIDINSTLHDLLKRYIRQPEFIQALAEPSLVIDNLPA
                     HVYFDLSSLNV"
     gene            7952..8488
                     /locus_tag="PKV31_04270"
     CDS             7952..8488
                     /locus_tag="PKV31_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNB92105.1"
                     /translation="MRIISGRFGGRHFYPPADKWPTRPTTDIAKEGLFNVLNNRLDWE
                     SVKMLDLFGGTGNHCFEFISRGCENTTYVDKFVGCIRFVHDLKKKLLLDKELTVVKSD
                     VFKYINSCTERYDYIFAGPPYPLHNIPDIPDLIFEKELLNPEGLFVLETNQMHNFRDH
                     VHFKELRKYGTTYFWFFE"
     gene            complement(8616..>9542)
                     /locus_tag="PKV31_04275"
     CDS             complement(8616..>9542)
                     /locus_tag="PKV31_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92106.1"
                     /translation="VTSSNFYPVGPSYTLYMPATVTMTAGMANDSFDVRIVPLGEHGV
                     MPIDANKCIKYQWEISHKNATGSANLKLQWASGTEGTTFNPNVGLYIGHWKTSANKFT
                     PIVEATYTSGDPSATSNTPFSEFSPFVIASEFIALPVQWLDIKAMRRDSRVLLQWKTA
                     SERDVKNFEVERRSADGSFTTIGTVMPQNKPEINAYSFVDERPSSGINYYRVRQVDAN
                     GQYSYSKVVQISFVDGVSFGIRNLGQQSFGFTGINGKAVMKVYDINGRLLLTKSVTND
                     DREFLLGFSSGSYAVTLEEGDNFYSGIFLLSR"
BASE COUNT         2635 a         1989 c         2143 g         2775 t
ORIGIN      
        1 ttctttaaga ttgtcgagca atttgttttc tgcatcttct aatctcgcat cgatggtgcc
       61 tttcatcgga taggtaaata tttcatcgtc ttttatcctg acaaaacatt caggagaaaa
      121 aaccacgaat tcatcccgat agagcaaccg ataaggcgca cgggcagtat ggtaaatatc
      181 tgacaagctg agattgcaat ctatgggtgt tggaaatgtt aggttgagga ggtaggaatc
      241 cccgtgtcgg atatgttcct tgaccaaccg gaatcgctct gagtaccatt ccctgctaac
      301 agggctgctt ttcagatcgg gaattgctga aatgggaata ttatccgtgt tgctgatgcc
      361 ggcgatatcg aagtgtaatc catgagttgc tgcatcttcg ggtttgcaga taacaggctt
      421 ttccatctca aaatccatga ggaagacaaa tggctgtccg gcagcagccc attcagaagc
      481 cttgtgcgaa aaatcctctc ttgtcatttt cctatattta caaagttctc caggatttgc
      541 cttcctttag cggtcagaaa ggattcaggg tggaattgta taccgaaaat ctgcttttta
      601 ttgtgctgga cagacatcag gcaatcctcc tgggacatgc ccgttactgc caactcatcc
      661 ggcaaattaa gatgatctat cttccaggaa tgataaagac ccacctctat cctgtccggc
      721 aaataccggt agatggatga acgattgtct atcgcaattc tttccctgtg gccatgtacg
      781 atcgtatcca tctgcacaag tctgcctcca aaatattcac agatggcctg atggccgaga
      841 cagataccga gcaacggttt atcaagtttt tcacaataag aaatgacatc tctaagctca
      901 ggaaagtccg aaggagtcat tgggccggga gatatgacga tgtggctgta atcttccaat
      961 acagcaatgt tgagaccttt tgaaggcaaa accccatata acacatcatc aagactgcgc
     1021 aataactcta caatgttgta ggtaaaggaa tcgttgttat ctatcaacag aatacgcatc
     1081 ttaagtttgc cattgctatt tagaagggca aaaataatct aaataacatt caggcaaata
     1141 ttcatttcgc gttgaggggg aatgaatatc aattcgtctt tgcatacaga cgcgatgagc
     1201 cttcatcgac cgtgtcaggt caatgataca gggaattact aatacattgt tgggcatttt
     1261 ataatattgg accgtgtaca aaatttttac tttaatgaga agggaagtaa aagggataat
     1321 ttcggcttga tctttagtct gccttgccat taacatgttg gaaaagcagt atctatacat
     1381 gagggtctga cggataagaa tggatctctt ccttaacata cattcaatga acattgtgcc
     1441 cgggttgagt tgaaatatgg atttacaggt ttattataag cacgatggtg gattcatttt
     1501 tgcctgattg cataactttt cctttcggtc gttgttttaa cccgaaacaa atccattgta
     1561 ttggtgcgta agattttcat tgttatactt atcctgtgca tcacaggtgt tgtttttagc
     1621 caatccaaac aaaataccat gacaaaggct gacaaaaata atccattatt gtgcaatccg
     1681 cagtccggga cttgtgaggt gcaggacagt atggttgcag attctgtccg gattaatatc
     1741 tcgccgtccg ttaccgacaa gataagggtg gtttatttta ccgatcctat ttgttcttct
     1801 tgctggggca ttgaaccaca gcttaagaaa ctgaagctcg agtatggcga ggttatcgat
     1861 gtgtcttatc acatgggtgg tctgttgccg gactggagtt acaacagtgg cggaataagc
     1921 aaaccttcgg atgttgcaag ccattgggat gaagtgagtc ttcattatga tatgccgatc
     1981 gatggtgatg tttggctgga ggatccattg tcgtcatcct atccgccttc cattgctttc
     2041 aaggcggccc agctgcaaga caaggataag gctttggatt ttctccggat aatcagagaa
     2101 atggtctttc tggaaaagaa aaacataacc cggctacaat atatgtccga ggcggcatcg
     2161 atggccggat tggatacggt acgatttaag aatgacatcg aagggagagc ggtgagcttg
     2221 tttgaagaag atctgcagtt ggcccgaacc tatggagtga gaggatttcc gaccatcttt
     2281 ctggaaggca agggtggcgc gagggaaatg gtatatagtg ccaggcccta cgccttttac
     2341 gaggtggcag tgctgaagct ggctcccaat gtagtcaagc aggagtatag caaagattgg
     2401 cagtttttat tttccaaata ttcttcactt accgttaaag agtttgctga gttgtcaggc
     2461 atgcggcgct ccgaatgtga ggcatttctc aacaatctgg ttgccgatgg acacttgacc
     2521 agactaacca ccaaaaacgg gtccatctgg cggcgcacag cgaaatgatt taaacacagg
     2581 attgtaggct gagtccgttg tagcgagaat gtttcccgga tttgatgaat tttcaaacta
     2641 ttgcgtttat aggggcacta ttattttcag gcttagattc cgtcccatat catatatacc
     2701 atcatgtcca ttaggcgaag aactataata ttcgaaatac tttaagcggt tcaaatgtga
     2761 tcggtaggct ttgtccagca tattattcaa ttgcaagata atttgaatgg ctttcgaatt
     2821 attgtattta acttctgcat ttaagcccag attgaaaagc gtatattccg gtgtaaaaga
     2881 ttcggatcca ttcagcgcta aataccgatt ttgggcgtgg ttatattcca tttcaaatcc
     2941 cggcgtaatc gaaagaagtc ttccggtgtg aggttttact tcgtattcaa tcctgctcgt
     3001 tacctttaaa gggggcatta atggtaaaaa ttcaccttca ttgcctttgc ctttaaattc
     3061 cggattttta tttaatccgt atacaatact caaactattg tcccaccgaa atccaacaag
     3121 cctttccggg tgaacagcaa accatagctc agatccaaat aagtgtgcag aagattgttg
     3181 atattggtac gttctgttgc cttgcgcatc agtaatcggg aagccctgtg catcagctac
     3241 ggttgccata tagatataat tttgaatgtt gttattgaag aaaccggcct cggccgaaat
     3301 gtttttatac ctcaggctga tactgaagtc ttcctgaagt gaaaactcag gcttgaagtt
     3361 tctgttacct aaatagatga tgtgtgcccc gggatcaaga ccattacttc caatctccgt
     3421 gatattgggc gaacggtagg ctctgccgat gtttgcttta aggttgatgt ttggggatat
     3481 cttataggtt gttccaatgc ttccgcttat cccatgaaaa gaattttgat attcagggaa
     3541 ttgcagattg gcttttgaat cattggcatg ggtttggtgt tcgaaccctg tttcaggatt
     3601 tatcccaaca taaaaatcgt tccactggac aagtcgtaaa tcatagcgaa ctccgccact
     3661 cacactccat tttcctttct ttatcctggc atataaatac atgcccccgt catataaatg
     3721 gtagtccggg atcggaaagt cagtcgcatc tttgtttttg ttgttttgca acattccatt
     3781 cacgccaagc gaggtttcaa tatttgcaaa ttttggtgca ttgtagcgga ttccataatt
     3841 tagggtattt aggcgcatgt acattccagc ctgttgaggc attgagggat gcgtatattc
     3901 tctacgggta ttttgttgtc cgcccaataa ataatctacc tctccttttc caatttgata
     3961 gaaaccttgt acataaattc tgtaatgctg aatatgttgt gccaaatccg gcactttgta
     4021 ggtattcagc tccttgtcag atactattgg ccttgtcttt aaatcatcat attccccttc
     4081 aaaaatttgt tttgtgaatt ttcgggtcaa tgaatccctg cttccatccg gtattccctg
     4141 attattgtcg taaagggtga aattcaaatg cgtataacct ttttttgatt tgaatccggc
     4201 aaaagccgaa aaattacttt cgttgaaatt agtcaaatag acccggccat ccgttggatt
     4261 tcgataattt tttgctgttc gatacgagcc acgcagtgca aacaatagac gttcgcggga
     4321 aaagttgagg gttacaccat tcccgatcag attattgttg gtttgatatt ctgagagata
     4381 ttttccataa aacgtccctt cattaccggt gggaattaca ggaaataaac taatgactcc
     4441 ggcaatagcg tcacttccat ataataaacc ggccggcccc ttaataactt ccgctttatc
     4501 aatattataa tgatcgacct ctaagccatg ttcatctccg tattgctggc cttcctgacg
     4561 catgccatca tagattgtga gtacgcgatt gtagcctaaa ccgtgaatga acggttttga
     4621 aatattaggc cctgttttta ccgcattcaa tcccggaaca ttttttacca atgcatctat
     4681 aatattgctc tcagtagctt tttctatttg tttgcgggaa atggcagtga tagagacagg
     4741 gttttcctta ataagtgttg ctcttgtagc accggtaatg acaatatctt ctaaaagcag
     4801 gttttcggcg tgtaaaaaaa tctggtaatt atccttatcg ggccttatcg gtatgctgtc
     4861 cggcctataa ccaatgctat gaaaaatcag gaaagactta tgtacagacg ctgaaatcaa
     4921 gaggttgaat cttccgtttt cgtcagagat tgttgcgaac tgatctttgg actggaaaac
     4981 attacaaaac tctatcgggc ccttagtgtc agcatccatt attgtccctg aaatggttct
     5041 gttttgggcg taaataaaag tattcacaga acagaagatc agcagaagca gagcattttt
     5101 catatttgta tttatatgtt tcatttgcaa aattataaaa gttagatata tctaactata
     5161 aatattagaa aaatgttttt atttttgtac tatggtatca cagacggagg aaaattattt
     5221 aaaggctttg tttcatctaa cttatgagac tgagaacaaa tcggaggccg gtacaaatga
     5281 gctggccaca taccttgaag tcaagccggc aactgccaat gacatgctca agaaattaaa
     5341 agataaaggg ttggtttctt acgagaaata cggaaagatt actttaacca acaaaggaag
     5401 caacattgca atagaaattg tgagaaagca ccgattgtgg gaaacattct tgtatgaaaa
     5461 aatgaatttt acttgggatg aagtccacga agtagcggaa caactggagc acattaaatc
     5521 tcagaaactg gtagaacaat tagaacgatt tttagatttt ccggaaatag atcctcatgg
     5581 agaccccata ccgaatgcaa ggggagaggt aaggaagatt gttcgtaaat cactttcaga
     5641 agtctgtatt ggagatgtat gtaagttagt tgcggtaaaa gatggtagtg tggcattttt
     5701 acaatatgtc gctaagttag ggttagggct gagcacaaaa ataaaggttg tcagtcgcca
     5761 ggaatttgat gggtcaatgg agatcgaggt caacaatcag cattttacgg ttagtcaaaa
     5821 gtttgcaaat aacttgtacg tcgtctgaat gttaagtagg tagtgtaatt gccattacaa
     5881 taagatgaag aagcgggaga tgttttagtt gtgtaaatgc aagtatgttg ggctgttatc
     5941 cgggatattt cggcagcctc tttatggggg atgaattgcc ataagtgaat ttgcaaaaaa
     6001 gttaatttta tttccggcga taaagtccct gctcaacccc gggcgctcgc ctccatatcc
     6061 tgacctgcaa agattcccgc aaggtactta taataagcgt tttggccgat catagcggca
     6121 ttgtctgtgc agaaagcaat ccgcggtata tagatgttcc agccttcctg ctcatgcatt
     6181 gtctccattg ccttcctcag tccgctattg gcagccacac ctccggccag ggctacctcc
     6241 cggatgcctg tctgatgtac ggccttttgg agtttgctga tcaggatgct tacgatacgg
     6301 ctttggacag aagcacaaag atcggtaatg ttttcccgga tgaaggcggg gttgtcagtt
     6361 gttgcctttt gaagaaaata aaggatggat gttttcaatc cgctgaagct gaagtccagg
     6421 cccgggatgt tgggttccgg aaaggaaaac accgggttgc cctgtgctgc gagcttatcg
     6481 atgatggggc cggcgggata atcaaggccc aggatcttac cggtcttgtc aaaagcttca
     6541 cctgcggcat cgtcaatcgt tgcaccgatg acttccacat cgagataatc ccgcacgata
     6601 ctgatctggg tatgcccgcc gcttaccgtc agacagagga atggaaatga aggtacaggt
     6661 gcttctatga aattggccat gatgtgagca tccatatggt gtacgtctat caggggtata
     6721 ttcagtgcca gtgccaggga cttggcaaag gtgaccccca catggagtga gcccagcaag
     6781 cccggagagc gggttatgcc gatagcgtcg atatccttca ggtccacaga tgcatccttc
     6841 aaggccttgt tgactgccgg catgattagt ctcaaatgcg cccgggaggc cacctcggga
     6901 atcacaccac cccaaagtgc gtgctgatcc tggctggaca cgacgttgga caataccttt
     6961 ccatcacgca caacggctgc tgatgtgtca tcacaggagc tttcaatcgc taaaattgtt
     7021 accggcataa caaaaaaatg aagtgcaaat ttacaagatt gattataagt acgccaaatt
     7081 gtattaatat tgtgtcttcg gaaccatatt tgatcatagt gccacaactc attacagaat
     7141 acaaacatcc tgtttccggc gtcataagca cttatggcag caacctcctt ttgctcagtg
     7201 agaacaatta cctggcttat tgtatagttg cagaggatgg acggtatgag ttactcagag
     7261 agtacaggat tgccacagga gaaaaattta ccgacttcca tcgggcagtt cttgcaacgg
     7321 atgaatatct gggcaatgcc ttccggagcg tatccatcgg gatttccaac aagcgaatgg
     7381 ttttggtgcc cggggcagtt ttcaaccggg aagaaagtgt ccgatatctc gaaagcggcg
     7441 cccctgtatg gtcaaccgat gaggtcaagg ccgattatct ggaaaaacag gacattcatc
     7501 tggtatatgc ctttcgttcc gctatttatc aggcataccg caatgccttc cccaacatga
     7561 aacttgagaa tgccctaagc acctatatta agcgacttga agccatcgtt gccctgcatg
     7621 acctcggcta caaagttttt gtcaatacct tgcatgatac catgtatatc gtagtgtatc
     7681 gcaatcgaca actttgctat gccaatgtat atgagtttgc aacggctgaa gactttcttt
     7741 attatatcat gctcatatat aaggaatttc agcttgaccc ggaaagtgta ccgacctatc
     7801 tttccggcaa aattgatatc aattcgacct tacacgacct gttgaaacgc tatattcgcc
     7861 aaccggaatt tatccaggca ttggctgagc cttcgcttgt cattgacaac cttccggcac
     7921 acgtgtattt cgacctgtca tcccttaatg tatgaggata atttccggaa gatttggcgg
     7981 cagacatttt tatccacctg cagataaatg gcccacgagg ccgacaaccg atattgccaa
     8041 agaaggattg tttaatgtgc tcaacaatcg actggactgg gaatcggtaa aaatgcttga
     8101 ccttttcggt ggtaccggaa atcattgttt tgagttcatc tcccgaggat gtgagaatac
     8161 cacttatgtc gacaaatttg tgggatgtat ccgttttgtc cacgatctga agaaaaaatt
     8221 gctattggac aaggaactca ccgtggtgaa atccgatgta ttcaaatata tcaacagctg
     8281 tacggaacga tatgactata tctttgccgg tccgccttat cctctgcaca atattccgga
     8341 tattccggat ctgatctttg aaaaagaact cttgaatccg gaggggctgt ttgtcctgga
     8401 gaccaatcaa atgcacaact tccgggacca tgtgcatttt aaggaactta gaaaatatgg
     8461 gacgacctat ttctggtttt ttgaatgaga ggagtcagaa tagttgacca ggcccctttt
     8521 gaatttaccg taaaatcgat gagacagcca aaacaaaaaa gggaacctgc aggctccctt
     8581 tcttgtttgc ttatatcgat cgcgagatag ccctgttatc tgctcaacag gaagatcccg
     8641 gaatagaaat tatctccttc ttccagagta acggcgtatg atccgctgct gaaccctaaa
     8701 aggaactccc tgtcatcatt ggtgacagat tttgtaagca ataggcgacc attgatatca
     8761 taaaccttca taacagcctt accatttata ccggtaaatc cgaaggactg ttgtcccagg
     8821 ttgcgtatgc cgaaggacac tccatccaca aaactgatct ggacaacttt ggaataacta
     8881 tattgtccat ttgcatccac ctgacgaacc ctgtaatagt tgattccgga ggatggacgc
     8941 tcatcgacga agctgtacgc attgatttca ggcttgttct gtggcatgac agtaccgata
     9001 gttgtaaaac tgccgtcggc tgacctgcgc tccacctcaa aatttttgac atctcgctca
     9061 cttgcagtct tccactggag aaggacccgg ctgtctcgac gcatggcttt gatatccaac
     9121 cattgaaccg gcaaggcgat gaattcagat gcaattacaa atggtgagaa ttcgctgaac
     9181 ggtgtattgg atgtcgcaga agggtcaccg gaagtatatg ttgcctcaac aattggagta
     9241 aatttattgg cactggtctt ccaatgaccg atgtagagcc cgacatttgg attgaaggtt
     9301 gttccttctg ttccggatgc ccactgcaat ttcagattgg ctgaaccggt cgcgttttta
     9361 tgactgattt cccattgata cttgatacac ttatttgcat cgatcggcat tacaccgtgc
     9421 tctcccaatg gcacaatacg tacatcgaat gagtcattgg ccataccggc agtcatggtg
     9481 acagtagccg gcatataaag agtgtaggat ggtcctaccg gatagaaatt ggaggaagta
     9541 ac
//
LOCUS       DAOQMC010000071         9529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2801_length_9529_cov_5.533777, whole genome shotgun sequence.
ACCESSION   DAOQMC010000071 DAOQMC010000000
VERSION     DAOQMC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2801_length_9529_cov_5.533777"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..1030)
                     /locus_tag="PKV31_04280"
     CDS             complement(392..1030)
                     /locus_tag="PKV31_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB92107.1"
                     /translation="MIKVHSFIFNSFLENTYILYDETKECIIIDPGCCEINENRTLSA
                     FIEKQGLKPVRLINTHCHIDHILGNDFVCVKYGLLPEYHKGEIPLVEAAPKIADMYGM
                     NYSPFPAAEKYLDEGDVIHFGNTSLEILFTPGHSPASLCFYSSSAGILIAGDVLFYGS
                     IGRTDLPGGDYDTLIRSINEKLMILPDETIVFPGHGERTTIGYERKSNPFLQ"
     gene            1131..4421
                     /locus_tag="PKV31_04285"
     CDS             1131..4421
                     /locus_tag="PKV31_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92108.1"
                     /translation="MYFVKTYRSYIIILIFTLLWQTWTFAQTNQVQFGKNRVQFHDDF
                     DQWLYYETNLFTVYWYGKSRNAGHKVVRIAEREFNDIIKTIDFKTNEKTEFLVFADIT
                     DLKQSNIGISDAFEYDGDITKTVDNKVFIAFDGNHNHLRRQIREGIAAVYLNNLLFGS
                     NLQEMVQNSLMLSLPQWFKTGLISYFGEEWSSHYDLRVAKVLSNKRIKDFEQLAAADP
                     RLAGQMFWNYVGFNYGKNAIGNLVYVTRINRNLDKGFQYVLGNSYDNIIQSVFKYYKS
                     RSDEHIKELKNIDRQWKPVTRMRTKEVVTAMELSPDGKSLLMVTNQISKIRVRLVDLK
                     TGKSKTLLKYGSKNKIQAPDPNYPCIAWKNDGKNILICYERFDKINLREYDVGTGKHR
                     SQLIPGRYQRLYRIAYWDDRNIVINGTTDGYGNIFLYKLSTRESTEITTDPFDKLDMT
                     VAGKGSSRSIYFSSNRPDASLTKVSGDSILPVGSFNIFSLQQVTADDGTSRWSMAQHT
                     FNDNFSERLPYVDEQGMLHFLTNEYKNNAIVRVGPDEKYMAGTSNVNLINLPVVIDQY
                     ALSGKTRKIYFNIFDGEQYRLTSMDADRWVASDVGLSKSPKEFDYTVPGDRQFQDSSA
                     VINARHTVFRKVETEIPENMRFITKYGDITEEETSETVRPERANEPKVSGANKMMPLN
                     PLNIVPYRLRFKITDSGVNFDNSMLFEGMNSYIGGGESNLFPPFGLLLKAQLRDMFED
                     YRLEGGVRIPVALNGNEAYLVYDDLKYRLDKRVALYRKASNERLNIGNISTQVRKNII
                     LMGQYDVKYALDQFNAVKVGLTLRQDRSFYKDTDLNSLDKPNETDQRVGIRVTYILDN
                     TLDKGLNLMAGTRIRVFGEWVKQFEISFDKRFNFNFNKGHMWVGGFDARNYMELGRHS
                     ILATRVAGQVSFGTERILYYLGGSDGWLFPGFNNETQTASDVNFAYQVPAPSLRGFQY
                     NIRNGNNYGLISNELRVPFLRYLSRRPISSTFLNNLQLVGFFDVGSAWTGWNPFTKYN
                     PLNTVYISQPGIDVKVNYFRNPIVFGYGAGIRFLLFGYFIRLDYARGWDTGNLTPPRM
                     YISLGTDF"
     gene            4844..5284
                     /locus_tag="PKV31_04290"
     CDS             4844..5284
                     /locus_tag="PKV31_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNB92109.1"
                     /translation="MKVLNLFAIILLITTSSFANTNENTDSGVSTKENYIHTMSHHGD
                     VISNPPGNITTTFKVAGNCGMCKKRIEAAAKSVKGVKAANWDGITEMITITYNEQKTD
                     LMEVHKKIAAAGYDTELVQATDESYNSLHGCCHYDRLQYDKKEE"
     gene            complement(5926..6900)
                     /gene="pfkA"
                     /locus_tag="PKV31_04295"
     CDS             complement(5926..6900)
                     /gene="pfkA"
                     /locus_tag="PKV31_04295"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNB92110.1"
                     /translation="MIKRVGVLTSGGDSPGMNAAIRATVRTAIYYDLHIYGFMNGYEG
                     LIDGSIKRLEKGDVANIIQRGGTILKTSRSARFMTKEGRQMAYETLLANDIDALVTIG
                     GNGTLAGAALFAEEFNIPIIGIPGTIDNDLSGTDYTIGFDSAINTAIEAVDKIRDTAD
                     SHNRLFFVEVMGRHSGYIAMFTGIGSGAGNVLVPEVDTNIDDLVKTLKLAQKRDKLFS
                     IIIVAEGSQLGSAHDVANKVLEHIKDVDAKVTVIGHLQRGGSPSCQDRVLSSRLGYAA
                     IYGLMHGESGVMAGVINNKVIFTPIKEALAQPKVFTDDFIKLANILAI"
     gene            complement(6919..7755)
                     /locus_tag="PKV31_04300"
     CDS             complement(6919..7755)
                     /locus_tag="PKV31_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92111.1"
                     /translation="MRIIVDLGGTKGDWCFILRDGRMESYVTRGFNPVLQSMWDLEQI
                     IKSELPHLTEPVEVMHYYGAGVHGETIYNSLYTTLKHLMHTDEVEIHNDLMAACRATC
                     QHESGIVSILGTGSASCLFNGTDIEDKIPSLGFIAGDEGAGSRIGKRVLQAYFYREMP
                     TDIQAEYEENYPLGREEILENIYKRPGANSYLASFANFALTQRRHPFMENIISEEFDR
                     FVVRHLLKYNNARQLKCHFVGSIAWFAQDILKASLERHNLTLGSVIRKPIDDLVRYHS
                     HS"
     gene            complement(8398..9093)
                     /locus_tag="PKV31_04305"
     CDS             complement(8398..9093)
                     /locus_tag="PKV31_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92112.1"
                     /translation="MAFRIFILLLFCSVAVSAQEVIVREEKIIRDGFHIAPVGFTSKG
                     YTVGVGYSRPIAPALMLHGTAGYMLSSYFEQEECSASGVQITPSLLYFPDNSDNYKGI
                     CLGLELPIFYYKVSRSWWVSNTSLNEISTFEYQKYHTANATAIQGGVAARFGFRTHKL
                     NHKFFWQPNISGGILFQKLNGYKEQYNYSSDNLPLNESLHRQQSGMAPYFRFEIAFGL
                     YRYKAETVKVIEM"
     gene            complement(9093..>9529)
                     /locus_tag="PKV31_04310"
     CDS             complement(9093..>9529)
                     /locus_tag="PKV31_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92113.1"
                     /translation="KSNPRFQNTVFHWPARDPVVVEGNYAYSTTRWGDEFQNPSGRLT
                     VLDVKDKANAFVIGTYDQKFPFGLGVSNGYLYVCNGHFGLNIFRIINGGQQLEFVKNI
                     ATDDIYDCIPSEELLVCQTKTGIWVFNIENPGDPVLTHKINN"
BASE COUNT         2667 a         2136 c         1997 g         2729 t
ORIGIN      
        1 cacaatttga aataattgtc aaatcctttg aaaatggtgc atctatcgat tttatttcca
       61 acattaccgg acttcctgaa agtaaaatta aagaaatatt gaatttgcga taatcgtttt
      121 gatggaactt tattgtcatt tatctattca aattcctaca cctccggtaa aaaattttag
      181 attttaaaat aaatcattca gagggtccta ttattaaatg tagctatgat atctgaatct
      241 ggcggacctt gctttgagta tcatcgtcca cttcagaaat tggatacctt atccacctgc
      301 ctccccaaag atagattttg agtcagtgtt ttgtagaacc ggatctgtcc tcttggtttg
      361 gctctcgcct gaaggaaata cagagatgcg cttactggag aaaaggattg ctttttcgtt
      421 catagccgat ggtagtcctt tctccgtgac cgggaaacac aatcgtctca tcaggcagta
      481 tcatcagttt ttcgttaatg cttctgatta gtgtatcata gtctcctccg ggaaggtctg
      541 tcctgcctat actaccgtag aacagtacat cacctgcaat gagaataccc gctgatgatg
      601 aataaaaaca caggctggcc ggggaatgtc cgggagtaaa gagtatttca aggcttgtgt
      661 ttccaaagtg aatgacatct ccttcatcaa gatatttttc tgcggccgga aaaggactat
      721 agttcattcc atacatatcg gctatctttg gcgctgcttc taccagagga atctctccct
      781 tgtgatattc cggcagcaaa ccatatttga cgcatacaaa gtcattgccc aggatgtggt
      841 caatgtgaca atgggtattg atcaggcgaa ccggcttcag tccttgtttt tcgataaatg
      901 cggataatgt cctgttttca tttatctcac aacaacccgg atcgataata atacattcct
      961 tagtttcgtc ataaagaatg tacgtatttt caagaaagga attgaaaatg aaagagtgta
     1021 cctttatcat accatttatt tacgacgatt tctcgtctcc tacatttact acaacacaaa
     1081 tatgtcaaaa aagtcataac tttagagtcg ataaatgata atttatttta atgtattttg
     1141 tcaaaacata tagatcttat atcattatat tgatattcac cctgttgtgg cagacgtgga
     1201 ctttcgcaca gaccaatcaa gttcaattcg gcaagaacag agtgcagttt catgatgatt
     1261 ttgaccagtg gctgtattat gagacgaacc tttttacggt atattggtac ggtaaaagtc
     1321 gaaatgcagg acataaggtt gtcagaattg ccgaaagaga atttaatgac atcatcaaga
     1381 ccatcgattt caaaaccaat gaaaagactg aattccttgt atttgccgat ataacggatc
     1441 tgaaacagag caatatcggt atttcagatg ccttcgaata tgatggtgat ataacaaaaa
     1501 ccgttgataa taaagtcttc atcgcatttg atggcaatca caaccatctg cgtcgtcaaa
     1561 tcagagaggg catcgcggca gtatacctca acaacctgct cttcggttcc aatctgcagg
     1621 aaatggttca gaacagtctt atgctcagct tgcctcaatg gtttaagacc ggattgattt
     1681 cgtacttcgg tgaggagtgg agctcccatt acgacctgag agtcgccaag gttctgtcca
     1741 acaaaagaat aaaggatttc gaacaactgg cggcagctga tcctcgtctg gcgggacaga
     1801 tgttttggaa ctatgtcgga tttaattacg gtaagaacgc catcggtaat ctggtatatg
     1861 taacccgaat caatcgcaat ctggacaagg gttttcaata cgtgttaggc aactcatacg
     1921 acaacatcat ccaaagtgtg ttcaaatatt ataaaagcag aagtgacgaa cacattaaag
     1981 agttgaagaa tattgatagg caatggaagc ctgtaacccg tatgcgtacc aaagaggtcg
     2041 tgactgcgat ggaactgtcg cccgatggta agagcctctt gatggtcacc aaccagatca
     2101 gcaagataag ggttcgcttg gtggacctga agaccggcaa atccaaaaca ttgctcaaat
     2161 atgggtctaa aaacaagatt caggcccctg accccaacta tccctgtatt gcctggaaaa
     2221 atgatggcaa aaacatcctg atctgttacg aacgatttga caaaatcaat cttagagaat
     2281 atgatgtcgg gaccggaaaa caccgtagcc agttgatacc gggcaggtac cagcgtcttt
     2341 atcgcatcgc ctactgggat gaccgaaata tcgtgatcaa tggaactaca gacggctatg
     2401 gaaatatttt cctgtacaaa ctctctaccc gggaatcaac agaaattacc accgacccat
     2461 ttgacaaatt ggatatgacc gttgccggaa agggcagcag tcgtagtatt tatttttcat
     2521 ccaatcgacc ggatgcatcc ttgaccaagg tttcgggcga ctccatcctg cctgtggggt
     2581 cattcaatat cttttcattg caacaagtga ccgcagacga tggcacatcg agatggtcca
     2641 tggcccaaca taccttcaac gacaacttca gtgagcgcct tccttatgtt gatgaacaag
     2701 gcatgctcca ctttctgacg aatgaataca aaaacaatgc catcgtcagg gtcgggccgg
     2761 atgaaaaata catggcaggc acctccaatg tcaacctgat caatctgccc gtcgtcatcg
     2821 accaatatgc actctccggt aaaacgcgga aaatatattt caacatattc gatggagaac
     2881 aatatcgcct tacttcgatg gatgcagatc ggtgggttgc ttctgatgtc ggcctgtcca
     2941 aaagccccaa ggaatttgat tatactgtcc cgggtgaccg acaatttcag gactcctctg
     3001 cagtgatcaa cgcacggcac acggtattcc gaaaagtaga gactgagatc ccggaaaaca
     3061 tgaggtttat caccaaatac ggcgatatca ctgaagagga aacatccgaa actgttcgtc
     3121 ccgaaagggc aaatgaacca aaagtctccg gagccaacaa gatgatgcca ctcaatccac
     3181 tcaatatcgt tccttaccgg ttacggttta agataactga ttccggagtt aattttgata
     3241 attccatgct ctttgaaggc atgaatagct atatcggggg tggtgagtct aatctcttcc
     3301 cgccattcgg cttgcttctg aaggcacaac tgcgggatat gtttgaagat tacagactgg
     3361 aaggaggtgt aaggattcct gttgccctca atggcaatga agcttatctg gtttatgacg
     3421 acctgaaata cagactggac aaaagggtgg cattataccg taaggcctcc aacgaaagac
     3481 taaacatcgg caatatttcc acacaggtca gaaagaacat catcctgatg ggacaatatg
     3541 atgtcaaata tgcccttgac cagttcaatg ctgtcaaagt gggcttgaca cttcggcaag
     3601 acaggtcttt ctataaggat acagacctca attcgctcga taaacccaat gagacagatc
     3661 agcgggtggg catcagggtt acctacatcc tggacaatac gctggataag gggttgaacc
     3721 taatggccgg aacaaggata cgggtctttg gagagtgggt caagcagttt gagatcagct
     3781 tcgacaagag gtttaatttc aatttcaata aaggccatat gtgggttggg ggttttgatg
     3841 cccgcaatta tatggagttg ggccgtcatt ctatcctggc gacccgggta gccggacagg
     3901 tgtcttttgg cactgaaagg attctctatt atctcggtgg aagtgatggc tggctttttc
     3961 cgggtttcaa caacgagacc cagactgcca gcgacgtcaa tttcgcctat caggtccctg
     4021 caccaagctt gcgcgggttt cagtacaata ttcgcaatgg taacaattac ggactgatca
     4081 gtaacgaact tcgtgttcct ttcctccgat accttagtcg acgccccatc agctctacct
     4141 ttttgaacaa tctacaattg gtgggcttct tcgatgtcgg ttcagcgtgg acagggtgga
     4201 atccatttac caagtataac ccactcaata cagtatatat ttcacagcca ggcatcgatg
     4261 tcaaggtcaa ttatttcagg aatcccatcg tttttggcta tggtgccgga atccgcttcc
     4321 tgctgttcgg ctacttcatc aggttagatt acgcccgggg ctgggacaca ggaaatctga
     4381 cgcctcctcg aatgtatatc agcctgggca cggattttta aacctgctga atcgtaacta
     4441 ttcacaacct atcagcctgg ccacatcgat aagctataga aaatggagaa cttaatatct
     4501 tgcactcata gaagcgtcat taaaaattat actaccttga tttgttggcg tttttttagc
     4561 ttggattatg caccattatc acataagaca tgaattataa tttgtttata accatccaac
     4621 tattcttccg gatagaaaat aagtatattt ggtgatatct tcgataaata ttggttcctg
     4681 aaagtctact tcagatccta ttattctgcg ctgaaagctt ggtctaactt gaatttaatg
     4741 gcaattaaac gattccctgt tcttttttgt taactttgta ttggttggat agtaacatgt
     4801 atcacaagac cgtttcttta ttttttattt aaataattat gtcatgaagg tattgaatct
     4861 ttttgcaatt attctattga ttacaacttc atcctttgct aataccaatg aaaacactga
     4921 ttccggagta tcaaccaagg aaaattacat acatacaatg tcgcatcatg gggatgtcat
     4981 ttcaaatcct ccgggcaata tcaccaccac attcaaggtg gccggaaatt gcggcatgtg
     5041 taaaaaacgg attgaagctg ctgccaaatc cgttaaaggg gttaaggctg ccaattggga
     5101 tggcattact gaaatgatca ctatcacata caatgagcag aaaaccgact tgatggaggt
     5161 acataagaag atagctgctg caggttatga caccgaactg gtacaggcaa ccgatgaatc
     5221 ctacaactca ttgcatggat gctgtcatta tgatcggctg caatatgaca aaaaagagga
     5281 atgatgtgcc tgtgaaaaaa catttgatta ccgcaatgga tgtctatagg ggtctgcccg
     5341 ctcatttttg cgaaatctga aagcacggtg tatgcatcta tatttttttg atggtttgtc
     5401 ttcaaaataa ttttgctact ccggaatgga gtcgcatatt atagctacag gcttcaacca
     5461 tgtcatctgt tttcgaactg aaactttatc atgtcgggac tatactcggg cattttgtgt
     5521 agtacttccg gtttttttca cccctggacc cgctcatgac caaatggcta ttccggtcct
     5581 ttacctcctg agtgcgtctg tatttgtttg tcatgaccaa cctgaatgta tgctttgatc
     5641 cataataacc atggttataa aatggcttct ggatggcttt cactacccgg gtgtggtgtc
     5701 ttcgggttat tgttgtagaa aattccatta cctcatagca tcaagctgct attggtcaaa
     5761 aaaatagctt tgaaaatatt taaattatgc tttcaggcaa cctttgtaaa tatcacaatg
     5821 caataaaggc cccgttcaca gatttgacat cagattctaa tcctacattc agtagttgac
     5881 acatctcaca ccaaccgcta caaccttcat tgaagtatct tttaattata tcgccagaat
     5941 attcgccagc ttaataaagt catcggtaaa taccttgggt tgtgccaacg cttccttgat
     6001 aggcgtgaaa ataaccttgt tgttgataac tcctgccata acacccgact cgccatgcat
     6061 caatccatag atggcagcat acccaagtcg actgctcagg acacggtcct ggcatgatgg
     6121 agatccgcct cgctggagat ggccgataac ggtaaccttg gcatcgacat ccttgatatg
     6181 ttcaagtact ttgttcgcta catcatgagc actgcccaac tgactgcctt ctgcaactat
     6241 gatgatagaa aacaatttgt cccgcttctg tgccagtttg agtgtcttta ccagatcatc
     6301 gatgttggta tctacttctg gaaccaatac gttgccggct ccgctgccga taccggtaaa
     6361 catggcgata taaccggaat gtcgtcccat cacttctaca aaaaagagcc tgttgtgtga
     6421 gtctgccgta tcgcgtattt tatcgacggc ctcaatggcc gtgttgatgg cggagtcgaa
     6481 gccaatggta taatccgtac ctgacaagtc attgtcaatc gtccccggaa ttccaatgat
     6541 ggggatgtta aactcttcgg cgaaaagggc tgcccccgca agagtcccgt taccaccaat
     6601 ggtaaccagg gcatcgatgt cattggccaa cagggtctca taagccattt gcctgccctc
     6661 cttcgtcatg aatctcgcac tgcgggaagt tttcaggatg gtaccgccac gttgaatgat
     6721 gttggcaacg tcgccctttt ccaatcgctt gatgctgcca tcaatgaggc cttcataacc
     6781 attcatgaag ccatagatat gcaggtcgta atagatggct gtcctcaccg ttgcacggat
     6841 ggccgcattc atgcccggtg aatctccgcc tgatgtcaac actccgactc ttttgatcat
     6901 gggtttgctt tttaaatgtt atgagtgact gtggtatctt acaagatcat caatcggttt
     6961 gcggatgaca ctgcccaggg tcagattatg gcgttccagt gaggccttca ggatatcctg
     7021 ggcgaaccat gcgatactgc ctacaaaatg acattttaat tgccgggcat tgttgtattt
     7081 aagcaaatgc cgcaccacaa aacgatcaaa ttcttcagaa atgatatttt ccataaatgg
     7141 atgccggcgc tgtgtcaatg caaaattggc aaatgaagca agataactgt tggcccccgg
     7201 ccgcttatat atattttcca ggatttcttc tctccccagt gggtagtttt cttcatattc
     7261 tgcctggata tctgtcggca tctcccggta gaaataagcc tgcaatacgc gcttgccaat
     7321 acgcgaaccg gcaccttcgt cccctgcaat aaaacccaat gacggaatct tgtcttcgat
     7381 atccgttcca ttgaagagac aggacgcgga tcctgtacct aatatcgata caatgccgct
     7441 ttcatgttga caggtagccc tgcatgctgc catgagatcg ttgtggatct caacctcatc
     7501 ggtatgcatc aggtgcttta gcgtagtgta cagggaattg taaatggtct ctccatgtac
     7561 tccggcacca tagtagtgca tgacttccac cggttcggta agatgcggca actcggattt
     7621 gatgatctgc tcgaggtccc acatgctctg caagacagga ttaaatcctc gcgtgacata
     7681 actttccatc cgtccatccc tgaggatgaa gcaccagtcg ccttttgtgc cacccagatc
     7741 aactatgatt ctcaatgccg ctaattttaa cgtgcaaaat tagtgttttt cttacactaa
     7801 gcaaagatta ttgacaaatt catagtgaag gcaatagcaa acttctttcc atctattagc
     7861 atttagtcct tcctgactcg gaatatccct gaatttctat ccattattcc ggattgacct
     7921 tttccactta tagtaggtat gacttgatga tgaatgattt ttgggtaagt ttgatatgta
     7981 cgtacaataa atcctttact tcctaaagta ttatggtccc attcgcgtca aattggcgct
     8041 atttcccccg gacgctttaa gctcccggag ctttaatgcc tgactaatac tgatgtttta
     8101 cagagccgct ctgccggccg cgttagcttc tgcaaatgcc gctacccggt tatgcataca
     8161 ctaattgact accaaaatat tcccacaatt gggttgaatc acgactaagc cgaataactc
     8221 gaattttatt gtgctttaca aatatagctt gccacaagag aaaatcaagt tcttgttaag
     8281 gcaaggtata tcttcccgtt ccaatgacct ccggtttttg tatgtgtacg ccatttacct
     8341 gtcaaataaa ggccctacat catttttgaa taggtgaata attacacccc ccggctccta
     8401 catctcgatt accttgactg tttccgcctt ataacgatac aggccaaagg caatctcaaa
     8461 cctgaaatac ggtgccatac cggattgttg cctatggaga ctttcattca aaggaagatt
     8521 atccgatgaa tagttgtatt gttctttata tccattcagt ttttgaaaca ggatacctcc
     8581 ggaaatattg ggttgccaaa aaaatttatg attcagttta tgggtgcgaa acccaaatct
     8641 ggctgccact ccgccctgta ttgcggtagc atttgccgtg tgatacttct gatattcaaa
     8701 ggtcgatatt tcattgagtg atgtgttgct tacccaccag gaccgactga ctttataata
     8761 gaatatcgga agttccaggc ccaggcatat tcccttataa ttgtcgctat tgtcaggaaa
     8821 atacaacaac gatggtgtta tctgtactcc ggatgccgag cactcctctt gttcaaaata
     8881 actgcttaac atatagcccg cggtaccatg cagcatcagg gctggtgcaa ttggcctgga
     8941 atacccgaca cccaccgtat aacctttgga agtgaagcct accggggcaa tatgaaaacc
     9001 atccctgatg attttttctt ccctgacaat gacctcctga gcgctgaccg caacggaaca
     9061 gaacaaaagc aggatgaaaa tacgaaaagc catcaattgt tgattttatg agtcaggaca
     9121 gggtcccccg gattctcgat attgaagacc cagatgccgg tttttgtttg acataccaac
     9181 agctcttcgc ttggaataca atcgtagata tcgtcggttg caatattctt gacaaattcg
     9241 agttgttgac caccattgat gattctaaag atgttcagtc caaaatggcc attacataca
     9301 tagagataac catttgaaac acccaggcca aatgggaatt tctgatcata ggttccgatg
     9361 acaaaagcat tggctttgtc tttgacatcc agcactgtaa gtcttccgga tggattctga
     9421 aattcatcac cccatcttgt tgtggagtat gcataattcc cttcgacaac gacagggtcc
     9481 cttgccggcc agtggaatac tgtattttga aatcttggat tcgacttgt
//
LOCUS       DAOQMC010000072         9205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2928_length_9205_cov_5.728306, whole genome shotgun sequence.
ACCESSION   DAOQMC010000072 DAOQMC010000000
VERSION     DAOQMC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2928_length_9205_cov_5.728306"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..889)
                     /locus_tag="PKV31_04315"
     CDS             complement(224..889)
                     /locus_tag="PKV31_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNB92114.1"
                     /translation="MKTKKLLVELVECVSLFESENPHLERYDLQGFMAFLQSRSVTGK
                     QEHFAPRKIAGHRKPHGFHLHENPANILSRLIGLIYRYAREYTKKALEGSSLQTVEEF
                     SFLVVLMTYDSLTKTELILKNAMVKTSGTEVVKRLLKKKLIRQFDDINDKRSQLVAIT
                     GQGLAEMKIVFPRMHLASDIIAGNLDPAELHTLVFLLQKLEAYHNELFLGRKDADLED
                     LMK"
     gene            1197..2654
                     /locus_tag="PKV31_04320"
     CDS             1197..2654
                     /locus_tag="PKV31_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB92115.1"
                     /translation="MKRLFLSNILILLVLNLLVKPLYIFGIDVQVQNSIGPEQYGLFF
                     SIFNYTLLFQFVIEYGITNYVKREVSLDNRRAPTVFSGILLLKLGLALLYFLLIIAFA
                     WAFNYTGIEIKLVLFVGLIQFLLTMVSFLRGIIIGMGRYRADSVFSVIDKAIMIVLLG
                     WKLYWPGTTLPDLNFFMMTYLLSVAITFFLALIFLFFVLKVQWARPDRAMIRQLLIAS
                     TPYALITFLMGAYGRLDAVLLHSLLADGDFQAGIYAAGYRMLDAYMMFALLFANLLLP
                     MLSSKQSDEEAFTDLMRFVTGLVMAATIIIGVGGFVFRREISELLYHHSDEAWYNTLG
                     LLFLTMIPVGLSTLYGTAILSIGRLKRMNILFSTGILLSIVLNLILIPEIKSEGAAVS
                     ALCVNSIIALGETIIFQRESGFKPGVSFLLKLMALLIISFAIFSGIYLIDTSFWLKIG
                     LGAIAMFLLAIALGMIQLNPRRIRAILSEKWTGTS"
     gene            complement(2697..3068)
                     /locus_tag="PKV31_04325"
     CDS             complement(2697..3068)
                     /locus_tag="PKV31_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB92116.1"
                     /translation="MPHKKFICAVIGLSDNPERYAYKAAERLLLHGYPVIGYGLRPCT
                     VMGISVITNKHAVTGIDTVTLYVGPARQDAWLPLILDLAPRRVIFNPGTENHIIEQKL
                     IDSGIEVIEACTLVMLATGEF"
     gene            3909..5024
                     /locus_tag="PKV31_04330"
     CDS             3909..5024
                     /locus_tag="PKV31_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92117.1"
                     /translation="MLFLLLFSLSGGEVIAMTFQRQLAIDTIRCDSASTLFADTINSL
                     IRKAEYREAFFMLDHWQEDCGENEINSRTSILLSMALGVHQDDAARQYFNNDFHFVFR
                     YRMEYSEMDDYQYNFWNHVTYFNLVPLRHPLDSALMGLAGRLYDKSSLLPNEKLVCRL
                     FMGDLRQFDKEVKKKGNSNTFISQYVKDDKRYISKQFLGVQFFSGIYASNDQGRFLGN
                     NASIGMGLYSPLKYRWQGNLQSRFRIMTHDNEFVFKALGNEYTVNSKLAFDFNINFMY
                     RYFENNHIAILPKAGIGLEYIGTGIKFYDEETEEPSSYNVSTMHTNIGVMALIPVFLR
                     NYAGIEVSYHYCPYGWSKGLISKFNNQAFTVELQFRF"
     gene            complement(5046..5945)
                     /locus_tag="PKV31_04335"
     CDS             complement(5046..5945)
                     /locus_tag="PKV31_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNB92118.1"
                     /translation="MDKKRVLIAGGSGFIGKNLYVELWNSKYDVFILSRNRKLCTRPD
                     FIYWAPENEIIEADGPLHFDCLINLCGEGIATKRWTAARKKVLMESRITPTRFIDSLV
                     RSGRLSITDYIGSSAIGIYGDRPGTSPCNETDVIEPKSFLADLCSQWEAAHLSLPESV
                     RKVILRIGLVLADEGGLLDQYRPLLSMRMVPYFGGGIPYMSWITAKDLCRMILFIMEH
                     PKTSGIYNAVADQPVISRDFAKAFIKSSGKKAFARGIPGFALKWMMGEQALLVLESQY
                     VSNKKIRMEGFRCLSTDISTAFG"
     gene            6020..6586
                     /locus_tag="PKV31_04340"
     CDS             6020..6586
                     /locus_tag="PKV31_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92119.1"
                     /translation="MLDLNVDNLLNKVHNYNAVFENTQKYRNVWRDSLKNQIISDVTE
                     ICKAVGLKIEIEATNQFSNLEAIICSLGNVKSGIHEIVEPDIEKHLIRFNGSLVYQQL
                     YNGKVLVVINYPIIENYMEPRPPKTIAIYRPEEIKTPFVLRHVETLVSEITAWEDYDD
                     DETVLPARIGFTHNLTPETTLKEDTQQQ"
     gene            7141..7398
                     /locus_tag="PKV31_04345"
     CDS             7141..7398
                     /locus_tag="PKV31_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92120.1"
                     /translation="MTWLASGLESACFYEKLLTITERKIGVNQAIAIKSTMPIHVPCF
                     RAKKPVVLSLVNEDIHVKMTPGFKQENIIFLLETFLIIFSN"
     gene            complement(7444..7779)
                     /locus_tag="PKV31_04350"
     CDS             complement(7444..7779)
                     /locus_tag="PKV31_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HNB92121.1"
                     /translation="MGKFEVKTRKNGEFQFSLLAGNGQTILSSEGYSTKAACMNGIES
                     VRKNSANDALFERKVGANGKDFFVLKAANHQVIGQSQLYESKASMENGIESVKKNAPD
                     ATVDDQTGA"
     gene            8150..>9205
                     /locus_tag="PKV31_04355"
     CDS             8150..>9205
                     /locus_tag="PKV31_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92122.1"
                     /translation="MKSYYLKNSIILMLFLITSSIYLIAQCPEGAITPTPGAALTIKS
                     GEMYCISSDISLLSVSIEEGGELILAPKVTVEANGIFNIDGYLTLYDYAGLKLTGAMN
                     QGSRYPADTKISIGNNAFISMTGSFTQFDPGANPAHPNAQAKIRLSNNAVMEICGTYT
                     QFSGNYPAVDYAGDMDHVAYFITKAPASGVGSSILSGDEETTWIAIGQVANLNPGDAS
                     YCGPNASPDKCDLWPSWLSAEYYNCFDAENNRDISLPIVLVSFDVVKNGLIASLTWAT
                     AVEYNHSGFEIQRSSDGINWSNLGFIAASQGFGVNTVTTRYAFDDLNPLPGNNFYRLK
                     QVDTDGSFSFSPVRTVGF"
BASE COUNT         2572 a         1995 c         1888 g         2750 t
ORIGIN      
        1 atttttggta tacttgtccg gatagctatg ctgctagagc tctgctctgt cccggaagtg
       61 aaacaatccg gggttccggg atgccgaggt tacagtcgta tagatcaatg aaattggtct
      121 ataacgaatg taccttcggt attaatatgg tcaggcattg aaatggtgaa ctttattttc
      181 ttccaaacaa taaattgtac aacataccga atttttactt caatcacttc atcaaatctt
      241 caaggtcggc atccttcctg cctaaaaaca gttcattgtg gtaggcttcc agtttttgca
      301 acaaaaagac cagtgtatgt aattcggcag gatccagatt ccctgcgatg atgtcggaag
      361 caagatgcat tctgggaaag actattttca tctcggccag gccctgccct gtgatggcga
      421 caagctggct ccgtttgtca ttgatgtcat cgaattgtcg gattagcttc ttcttcagca
      481 atcgtttgac tacttccgta ccggaagtct tgaccatggc gttcttaagt atcagttctg
      541 tcttggtcag actgtcatag gtcatcaaga cgacgagaaa ggaaaactcc tccacggttt
      601 gcaaggaact tccctccagt gcctttttgg tgtattcacg tgcatagcgg tatatgagtc
      661 caataagccg ggaaaggatg ttggcgggat tttcatggag atggaagcca tggggtttcc
      721 tgtgtccggc tattttgcgt ggggcaaaat gttcctgttt gccggtcaca ctgcggcttt
      781 gaagaaatgc catgaagccc tgcaagtcat atcgctccag atgaggattt tcagattcga
      841 ataggctgac acactctacc aattcaacca gcaatttctt tgtcttcata caactgacac
      901 attatttaca caaagatagg acaaatggta tgttttcgta ctaaatttga taagataaag
      961 gaagcttagc tggtgacagc cctcatttaa aaaactttct gactcgtcat caacaagtgc
     1021 aaattgacat ccattccttt ctacatgtgt taaaaaattt acctgagcca attaagtatt
     1081 ttgaagagta tatctatctt gatgcgaagc atgtcattca aattcacatc ttatcaaccc
     1141 ttgattttta tttggaatta ccgttcaatt catccgtgcc aactatcttt gaggtgatga
     1201 aaaggctttt cctgtccaat attcttattt tactggtgct taacctgttg gtcaaacctt
     1261 tgtatatatt cggtatcgat gtccaggtac agaatagtat cgggcccgaa caatacggtt
     1321 tatttttcag catcttcaac tataccctgc tgtttcaatt cgtcatagaa tatggcatca
     1381 ccaattatgt aaagcgggag gtcagtttag acaaccgtcg ggcgccgaca gtcttttcag
     1441 gtatattgtt attgaagctc gggttggcgc tgttgtattt cctgttgata atagcctttg
     1501 cgtgggcttt taattatacc ggtatcgaaa tcaaattggt tcttttcgtt ggtttgatac
     1561 agtttctgct tacgatggtc tccttcctca ggggtattat cattggcatg ggccgatata
     1621 gggcggatag tgtgttttcg gtcattgaca aggccatcat gatcgtcctg ctgggatgga
     1681 aactgtattg gcccggaacg acacttccgg atctcaattt tttcatgatg acgtatttgt
     1741 tgtcagttgc aataaccttc ttcctggcgt tgattttcct tttctttgta ttgaaagtcc
     1801 agtgggcaag gccggatcgt gctatgatca ggcaattatt gattgcatct acaccatacg
     1861 cactgatcac ttttttgatg ggtgcctatg gaagactgga cgctgtgttg ctgcatagtc
     1921 tgttggcaga tggtgatttt caggcgggaa tttatgcggc aggttacaga atgctcgatg
     1981 cttacatgat gttcgcatta ttgtttgcca atttactatt accgatgtta tcttccaaac
     2041 agagtgatga agaagctttt acagatctga tgcgtttcgt caccgggctg gtgatggcag
     2101 caacaataat catcggtgta ggaggttttg tttttcgacg tgaaatctcc gagttgttgt
     2161 atcaccacag cgatgaagcc tggtacaaca ctttggggtt gttgttcctg acgatgattc
     2221 cggtgggact aagcacactg tatggcaccg ctattctgtc tattgggcgc ttgaaaagaa
     2281 tgaatatcct tttttccacc ggaatcttgt tgagtatagt cctcaatctt atactgattc
     2341 cggaaataaa gtctgaagga gctgccgtca gtgccctgtg cgtcaattcg atcatcgcat
     2401 tgggggaaac cataatattc caaagagaat ccggatttaa gccgggagtt tccttcctgt
     2461 taaaattgat ggcattattg ataatcagtt ttgcaatatt ctccggaata taccttatcg
     2521 atacttcctt ctggctaaaa atcgggttgg gagcaattgc aatgttccta ttggcaatag
     2581 cattgggaat gattcaactg aacccccgta ggattcgggc catcctatca gaaaaatgga
     2641 ctggaacctc ttgaacattt gatcactgca ttagtttcgg aaaatgcacc gatgattcag
     2701 aattcgccgg tggcaagcat gaccagcgtg caagcttcaa taacttcaat gccagagtca
     2761 ataagttttt gttcgatgat atgattctcg gtgcccggat tgaagataac acgtcgtggt
     2821 gcaaggtcca aaatcaaagg gagccaggca tcctgacgtg caggaccgac atagagggtc
     2881 actgtgtcga taccggtgac ggcatgctta ttggtgatta cggatatgcc cattacagta
     2941 catggtctca ggccgtaacc aattacaggg tatccatgca aaagcagtct ttcagcagcc
     3001 ttgtatgcat acctctcggg attgtccgaa aggccgatga ctgcgcagat gaattttttg
     3061 tggggcatat gttacttaaa acacaggatg gcctaaatgg ttttgggaca gaaagggatt
     3121 gagaataaag aggtttgccg gccggggaag acggggtttg gggtgtcgtc acgaagtagg
     3181 tagaagaata ataaccgctc cggtaaacac agcaagtaat cccggggatt caatttgaaa
     3241 atttgcttta tcaggcaggc tttatttatt tcatccttct gaagtgcaga cataccgatg
     3301 gtttcccgct attatttatg tacatacttc attttttccg gcccccaacc cgacaatcgc
     3361 ttcccgacat gatcaaaatg attccggcaa aaacggcttc accgaagtag gtagaagaat
     3421 aataaccgcg ccggtaatca cagcgagtaa tcccggggat tcaatttgaa aattagcttt
     3481 atcgggcagg ctttatttat ttcatccttc tgaagtgcag acataccgat ggtttccggc
     3541 tattatttat gtacatactt cattttttcc ggaccccaac ccgacaatca cttcccgaca
     3601 tgatcaaaat aattccggca aaaacggctt caccgaagta ggtagaagaa taataaccgc
     3661 gccggtaatc acagcgagta atcccgggga ttcaatttga aaattagctt tatcgggcag
     3721 gctttattta tttcatcatc ctgaagtgca gacatactga tggtttccgg ctattattta
     3781 tgtacatact tcatttttcc cgcacaccaa cccgacaatc aatagataat cattccccct
     3841 atcctccaat tattactgcc gcaattctca ccttttcgtt aattttggac atgatgaaaa
     3901 attggatttt gcttttttta cttctgttct ctctttccgg tggagaggtt atcgcaatga
     3961 cgttccaacg acaattagcc atagatacga tacgttgcga ctcggcttcc accctgtttg
     4021 ccgataccat caatagtttg attcgcaagg cggaatatcg agaggctttc ttcatgcttg
     4081 accactggca ggaagattgt ggtgaaaacg aaatcaatag ccgcacttcc atccttcttt
     4141 ctatggctct tggagtccat caggatgatg ctgcacgaca atatttcaac aatgattttc
     4201 actttgtttt cagatatcgc atggaataca gtgagatgga cgactaccaa tacaatttct
     4261 ggaatcatgt gacctatttc aacttagttc cgctgcggca tccgttggat tcggcattga
     4321 tgggcttggc tggcaggctg tacgataaat catccctcct acccaatgaa aagttggtgt
     4381 gccgattgtt tatgggtgat ttgagacaat ttgacaagga ggttaagaaa aagggaaact
     4441 ccaacacctt catcagccaa tatgttaagg atgacaagag atatatttca aaacagttct
     4501 taggcgtaca gtttttctcc ggaatttatg ccagcaatga ccaaggtcgg tttttaggca
     4561 acaacgcttc catcggcatg ggcctttact cgccattgaa atacagatgg caaggcaatt
     4621 tacaatccag attcagaatt atgacccatg acaatgaatt tgtcttcaag gcattgggca
     4681 atgaatatac tgtcaattca aagctggcat ttgacttcaa tatcaacttc atgtaccgct
     4741 atttcgaaaa caatcatata gccatacttc ccaaagcagg tattggtctc gaatacattg
     4801 gaacgggaat aaaattttat gatgaggaaa cagaagagcc ttccagctac aatgtatcca
     4861 ccatgcatac caatataggg gtgatggcct taattcccgt cttcctgcgc aattatgccg
     4921 gaatagaggt gtcataccat tattgtcctt atggctggag taagggatta attagcaaat
     4981 tcaacaatca ggccttcaca gtcgaattgc agtttcgatt ttaagaattt attttaacct
     5041 aatggtcaac caaaggcggt actaatgtcc gtactcaggc accgaaatcc ttccattcta
     5101 attttcttgt tggaaacata ttggctttcc aacacaagca atgcttgttc tcccatcatc
     5161 cacttcagtg caaatccggg aatacctctt gcaaatgcct ttttaccact acttttaata
     5221 aatgcctttg caaaatcacg acttataaca ggctgatctg ccacggcatt ataaattccg
     5281 ctggtcttag gatgctccat tatgaagaga atcatccgac ataggtcctt tgccgttatc
     5341 caggacatat aaggaattcc tccaccgaaa taagggacca tacgcataga gagaaggggt
     5401 cggtattggt ccagcaggcc gccttcatca gctagtacaa gcccaattcg taaaatgact
     5461 ttacgaaccg attcagggag cgatagatgc gcggcctccc attgactaca caaatctgcc
     5521 aagaaacttt tcggttctat gacatctgtc tcattacaag gagatgtccc cggtcggtct
     5581 ccatagatcc cgattgcaga agatccaata taatccgtaa tactgagtct tcctgaacga
     5641 accaacgaat caatgaagcg tgtcggtgtt atcctgctct ccatcagaac cttcttccgg
     5701 gctgcagtcc acctttttgt ggcaatgccc tctccgcaaa gattaattag acaatcgaag
     5761 tgaagtggac catccgcttc gatgatctca ttttctggtg cccaatagat gaagtccggt
     5821 cgggtacaca gttttctatt acgcgacaaa atgaaaacat catacttgct gttccacaat
     5881 tcaacataga ggtttttccc gataaacccg ctgccccctg caatcaagac tcttttttta
     5941 tccattttgg caaaattcac aaaattggat aaatatatgt acattagtcc aataatttaa
     6001 aaactataaa acgaaagtga tgctcgacct caatgtggac aatctgctca acaaggtaca
     6061 caactacaac gcagtattcg aaaataccca aaaataccgc aacgtttggc gggacagcct
     6121 caaaaatcaa atcatctcgg atgtcactga gatatgcaag gcggttggct tgaaaatcga
     6181 gatcgaagcg accaatcaat tcagcaacct ggaggctatc atttgctccc tgggtaatgt
     6241 caagtccggc atccacgaaa tcgtagaacc cgatattgaa aagcatctga ttcgcttcaa
     6301 tggaagtctg gtataccagc aactttacaa tggaaaagtt cttgtagtaa tcaattatcc
     6361 catcatcgaa aactatatgg agccccgacc acccaagacc attgccattt accgtcctga
     6421 ggaaatcaag actccctttg tgctgaggca tgtggaaact cttgtttcgg agataaccgc
     6481 ctgggaggat tatgatgacg atgaaaccgt attgccggca cgtattggtt ttacgcataa
     6541 tcttactccg gaaacaactt tgaaggaaga tacgcaacag cagtgatgat aagatgttcc
     6601 ttgcatttta atcacatttc ctgaaatctt cgaatgccgg gttaaatcca ctcgcgcatc
     6661 gcagttgagg tctgcaggca aagtaatcaa tacttcaaaa taaaatttaa ttgcagtcgg
     6721 gtgtggatag gtagcctggg acattggtca ttaaagatac agaaacagcg tggcagtaca
     6781 cgggaactgc cggttagata ttgtcgattg ttggacaacc accattctcg caggaatttc
     6841 attcgtgatc ggttgaataa gtcttatccc aaaaggcaat cgttttcaac cttattacgt
     6901 aaaacaatga tgactcaacg ggatttctaa gtatggtatt tttatgcctg tttgatgaat
     6961 tacctttgaa tcgggtcttt tcagaaaatg gctcaatgga aataggatcc caaaccttac
     7021 aaaatgtgat attaaaaaac aaaacagctt taatctttca cctggcaatg attcttctta
     7081 ctattaccat catcagcgtg tcaaaaagtc attcctaggg cgctctactt aaaatctcag
     7141 atgacttggt tagcctcagg attagaatct gcttgctttt atgaaaaatt gttgaccatc
     7201 acagagagga agattggtgt aaaccaagca atagccatca aaagcacgat gcctattcac
     7261 gtcccgtgtt ttcgtgctaa aaaaccggtt gttttatctt tggtcaatga agatattcac
     7321 gtaaaaatga cgcctggctt taagcaagag aatatcattt ttctcttaga aacctttctg
     7381 attatcttct ccaattaggt atatagatgt aaaaaaagcc cgggagaaaa cccggacttt
     7441 ttatcatgca cctgtctgat catcgaccgt ggcatcagga gcatttttct taacagactc
     7501 aatgccattt tccatggaag ctttgctttc atacaattgg ctttgtccta tcacttgatg
     7561 attggctgct ttcaatacaa aaaaatcctt tccattggct ccgaccttcc gctcaaaaag
     7621 ggcatcattc gcagaattct ttcttacaga ttcaatgccg ttcatgcaag ccgcttttgt
     7681 agaatatcct tcgctactca gtatggtctg gccattacct gcaagcaaac taaattgaaa
     7741 ctctccattt ttccttgtct taacttcaaa ttttcccata gattattctt ttgtttaaat
     7801 gataaaaacg aaaaagatac aatattatga cgacattgca ttcataaagt catcatttat
     7861 ttcaaaacaa gcttatcata cttttgtttt tattcgttca aattgcccgg ttttccaaaa
     7921 tataataagg ggtacatccc ggttctttcc acattaacaa atagttattt ataaatttat
     7981 tgctcatata attatgatta tcatgcgttt ataacacata attcaattgt ttaaattttt
     8041 ttctcgtttt tactgtaccc cttatcaaaa taaatcgtct aatagggctg ataaacaaaa
     8101 taatgttttg aaacaaaaat actattcata aagcaaaatt atttttgtca tgaaatccta
     8161 ctacttaaag aactctatta tcctgatgtt atttctgatc acctcttcca tctaccttat
     8221 tgcccaatgc cctgaaggag cgataactcc aacacctgga gcggccctga caatcaaatc
     8281 cggtgaaatg tattgcattt catccgatat ttcattgcta agtgtatcca ttgaagaggg
     8341 tggcgagctt atacttgcgc caaaagtaac cgttgaggcg aatggcattt tcaatattga
     8401 cggctatctg accctgtatg attatgccgg tttgaagctc acaggcgcca tgaatcaggg
     8461 cagcaggtat cctgccgata ctaagatttc catcggcaac aacgccttca tctccatgac
     8521 aggatctttt acacagtttg accctggagc gaatcctgcc catcctaatg cacaggctaa
     8581 aatccgactg agcaataatg ccgtaatgga gatttgcggt acctatacac agttctccgg
     8641 caattacccc gcagtggatt atgccggtga tatggaccat gtcgcttact tcatcaccaa
     8701 agctccagca agtggtgtcg gctcatccat actttccggg gatgaagaaa ctacctggat
     8761 tgccatcggc caagttgcaa acctcaaccc gggtgatgct tcctactgcg ggccaaacgc
     8821 ttcaccggat aaatgcgacc tatggccatc ttggttatca gccgaatatt ataattgctt
     8881 tgatgccgaa aataatcgag acataagcct gcctattgtt ttggtcagtt ttgatgtagt
     8941 gaagaatggt ttaatagcat ccttgacctg ggcgactgct gttgaataca accatagtgg
     9001 ctttgagatt caaaggagtt cagacggaat aaattggtct aaccttggct tcatcgctgc
     9061 ttcacaaggc tttggtgtca atacagtcac gacacgctat gctttcgatg atttgaatcc
     9121 actgccgggc aacaattttt acaggctaaa acaagtggat actgacggta gcttctcttt
     9181 cagcccggtc agaactgttg gtttt
//
LOCUS       DAOQMC010000073         9147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2959_length_9147_cov_4.754839, whole genome shotgun sequence.
ACCESSION   DAOQMC010000073 DAOQMC010000000
VERSION     DAOQMC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2959_length_9147_cov_4.754839"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKV31_04360"
     CDS             complement(<1..434)
                     /locus_tag="PKV31_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB92123.1"
                     /translation="MVQESTFCVVTPVFNDWDCFERLYVELKAMFAEQGHRFMVLAVN
                     DGSTLPFPEHFKVIQNIDIEDRVSWCEECILVLDLQRNLGHQKAIAIGISFVERHITP
                     DYCIVMDSDGEDRPSDIPHLIKADHDGSKIIFARRRSRQESR"
     gene            complement(813..1397)
                     /locus_tag="PKV31_04365"
     CDS             complement(813..1397)
                     /locus_tag="PKV31_04365"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNB92124.1"
                     /translation="MINLILFGPPGSGKGTQAVRIAEKYNLVHISTGDLFRYELNNMT
                     ELGKLAKSYMDKGQLVPDEVTVDMLKSKMDRHPDANGFIFDGFPRTLNQADALMSLFK
                     ARKTSLTAMLALEVDEDEIVRRIKMRGETSGRADDNDEDIIRNRFAVYKNETQPVFNY
                     FKNLGLATSINGIGDVDKIYHLLVNRIDRALNRL"
     gene            complement(1733..2989)
                     /locus_tag="PKV31_04370"
     CDS             complement(1733..2989)
                     /locus_tag="PKV31_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB92125.1"
                     /translation="MKGLRIHKPKPLSIPSVEKVVLNNGLKLYFLEGGAQEVVKFEWC
                     FKAGRSSEKSRLASVLCHSLIKEGCEGYSGFQIAEHFDYFGSSLNHQAGFDYCSFSFY
                     CLNRFFDPLLDMFLKVISKPSFPENELSLLKSKHISRLRQERDDADFSAYKILTEKIF
                     GTEHPYGYNSTEELVHHMHTDLLRLHFESNYLTSEDFVMITGRLMPGSKQELTDRLSS
                     VKLHSPPENNTRLVEKSEAEKYLDNSFSKNQCAFKLGQRTIKRDHEDFPAFYIFNHLL
                     GGFFNARLSRIIREQKGLTYNILSTHDTYVHDGIFYISAESSWKNLPELRNEVYRQLD
                     NVLKKPPSKAELERVRAFLIGVLITSFDGVFATADTFRSCLVEEVDLISLQPLMDAIV
                     KMSAEEVFERSHRHLDPARMIEVVTG"
     gene            complement(2986..3624)
                     /locus_tag="PKV31_04375"
     CDS             complement(2986..3624)
                     /locus_tag="PKV31_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92126.1"
                     /translation="MNFYLNMFSRKTFLLGLMVLTFAACKRDSNTDEVNTDVVELQGV
                     KTSTPDTSFGFSKKLATENLHISIKTKDFEGGKMINLIISQRDGGNINKKIKIDGEII
                     DAFTADLNGDGKDEVYIFNQGEGSGSYGNLYGYQAETSGLDSISMGDLPAQYRDKYMG
                     HDSFAIDGKQLLRFIPLYNEIDPTCCPTGGKATIKYKLANVKGKLVLVAEQK"
     gene            3804..4730
                     /locus_tag="PKV31_04380"
     CDS             3804..4730
                     /locus_tag="PKV31_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92127.1"
                     /translation="MINFSPEYDILFYTFAHSFEMRFHITGTYLLTTILLTTLAGCSK
                     EESSVPTYLSFGQANIIFQQNAGSNHQNIEDYWLIINGQTIAVASPDRTVPVIGNGIQ
                     EITVQPGIRVNGQRDEAQIYSFLEPYKTTVSLCACADTIHLIPEFKYKPTALFSFIED
                     FENSNRFSVDLDDNIATSSTTQTQTVAEGSFSLALNVDATNKINSVTTQDFYTDFESN
                     GRDLFLEFEYKSDFTFLIGLQANTGSGHFTEYAAGAYATPTWKKMYVNFTDLALASKS
                     TGGYRPVFTINHAGGTQGGGNLYLDNIKLVHY"
     gene            4737..6137
                     /locus_tag="PKV31_04385"
     CDS             4737..6137
                     /locus_tag="PKV31_04385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNB92128.1"
                     /translation="MNNTRKRDLLVYGICDALMAFTAWAIFSYKFVINRNFVLLENPT
                     LQAWGTAGLIVVGFILLYTIFDFYTDLYRQSRLATLTRTIFISLLGCLLVVLLIRDVF
                     MGNTISLWFHFFLWHFFLTALSRMILLTRASRRLKAGSVTFNTIIIGGDENALELYTE
                     IKSRQKGLGYKFIGFIDSNGSSRNLLEGHLPLLGKLDQLAAIIVQHKVEEVIIAIETQ
                     EHDKLRDILNVLFDFSSNILIKVIPSMYDIMLGTVKMNHVYGAVLIEIEQVMMPKWQV
                     LVKRGLDIVISIIVIVLLSPMYLYIYIRVKMSSPGPAIFLQERIGLNHRPFSIYKFRS
                     MYTNAEEDGPQLSNENDARCTPWGAVMRKWRLDELPQFFNVLKGEMSIVGPRPERRFY
                     IEKIMERAPHYKHLLKVRPGITSWGQVKYGYASNVEQMIQRLKFDILYIENMSLALDF
                     KIMFYTLLVLFQGKGK"
     gene            complement(6386..6739)
                     /locus_tag="PKV31_04390"
     CDS             complement(6386..6739)
                     /locus_tag="PKV31_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92129.1"
                     /translation="MERIQAQRVAYITNELSLTPAESEKFWPVYKEYKQKVHQLNQSQ
                     RPSKPIEQMSDAEKTSFINTMLDNEIKAAEIKRDYYVSFQKILPVAKVAKLEMAERSF
                     KKELIGKMAGENRRR"
     gene            complement(6845..7285)
                     /locus_tag="PKV31_04395"
     CDS             complement(6845..7285)
                     /locus_tag="PKV31_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92130.1"
                     /translation="MEKDIKNLRKDNFRIPDGYFDEVQMKISARLEQGNGRILPLESG
                     KGLSPGIVKIRKLVFIMGVAAACLAGFIIFKPQSVPAHEINLNGISNEDIATYLKENV
                     MDIDLEMIANTDPTLTATSNNPDNLSDEEYDLLIDEISLDDIID"
     gene            complement(7290..7832)
                     /locus_tag="PKV31_04400"
     CDS             complement(7290..7832)
                     /locus_tag="PKV31_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB92131.1"
                     /translation="MTDEQLVEMLLDKARQEQGFRMLMHQYRERLYSVIRKIVLTHEN
                     ADDVFQNTMIKIFTHIGQFGNKSSLFTWMYRIATNEALSFLRRQKKVGADGEVKPELT
                     EMWLQADPFFDGDELQIELMKAVALLPSKQKEVFNLRYFDEMKYSDLSELLGVSEGAL
                     KATYYHATKKIEAYFSQLNK"
     gene            complement(8251..9051)
                     /gene="pyrF"
                     /locus_tag="PKV31_04405"
     CDS             complement(8251..9051)
                     /gene="pyrF"
                     /locus_tag="PKV31_04405"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNB92132.1"
                     /translation="MNQRQLTELIRSKGSFLCVGLDPDMSRIPAPYSDADDSLFEFNK
                     MVIDATRDHCVAYKPNTAFFEAHGASGWLSLEKTIRYIGSSHFVIADAKRGDIGNTAN
                     MYAEAFFKELDVDAITLSPYMGHDSVSPYLKYPDKWAIILVKTSNTGSSDFQTLVTEN
                     DSPLYIDVLRKCLAWGDIDNTMFVVGANNLSELKGIREIAPDHFFLVPGFGAQGGQLK
                     DIQPYLSSDTVGILANYSRQVIYAGDFAQINEAAAAIHRQMREMLKTL"
BASE COUNT         2710 a         2001 c         1676 g         2760 t
ORIGIN      
        1 atacggcttt cttgcctgct tctcctgcgt gcaaatataa tcttcgaccc atcgtgatcc
       61 gctttaataa gatgtggaat gtctgaaggt ctgtcttctc catctgaatc cattacaata
      121 cagtaatccg gagtaatatg tctttcaacg aaggagatgc caatagcgat ggccttctgg
      181 tgaccaagat tccgttgaag atcaagaact aaaatgcatt cttcacacca ggagactcta
      241 tcctcaatgt ctatgttttg tattaccttg aaatgctcag ggaaaggcag tgtgctgcca
      301 tcattgacag ccaataccat aaacctatgg ccttgttcgg caaacattgc tttcaactca
      361 acatacagtc gttcaaagca atcccagtca ttgaagacgg gcgtaacaac acaaaatgta
      421 ctttcctgta ccatctatcc ttaaaaaaaa gcacaaaata agcaaaactc tcggattgac
      481 gtcatagaaa tgccaaaggc ataaggtgga aatattgcaa gtcaaagacc gggtcggagc
      541 cacttgtcac tgaaattatc tgtcggatga tgataaaacc actatagaaa aagttgctga
      601 atgaaactgg ccaggacaac gaatgcacta tcctacattc aacataatgc atccattaat
      661 tactataaac ttggtgtatc cttaaaatgt ttgttgggaa agctttatta tccaatgttg
      721 ataaaacttt ttcaaatttg tcttcctaaa ttctagttag ggcatcaaat ataaattcac
      781 cccattaagc ccaacaatgt cggaaacaat aattacaacc tatttagtgc ccgatctatc
      841 ctgttcacca gcaaatggta aatcttatcc acgtcaccta tcccattgat ggaggtggcc
      901 agtccgagat tcttgaaata attaaacacc ggttgagttt catttttata aacagcgaac
      961 cggtttctga taatgtcctc atcgttgtca tctgcgcgac ccgatgtctc cccacgcatc
     1021 tttatcctcc gaactatctc atcctcatca acttccaaag caagcatagc ggtcaaagat
     1081 gtttttctcg ctttaaacaa tgacataagg gcatcagcct gattcagggt acgcggaaat
     1141 ccgtcaaaaa tgaagccatt ggcatccggg tgcctgtcca tcttactttt cagcatatca
     1201 acagttactt catccgggac caactgacct ttatccatat aactctttgc caacttgccc
     1261 aattctgtca tgttgttcaa ctcatatctg aataaatctc cggtggagat atgcacaagg
     1321 ttgtattttt ccgcaattcg cactgcctgt gtccctttgc cggaacctgg aggaccaaaa
     1381 agtatcaaat ttatcatgat aacaggatgt tatttaaagc cgaaaataga aaaattattt
     1441 gaaagtttac aattctttga aaatttagaa tatatcacaa attgcatctc acttatttca
     1501 gaaagaacag tttggcagct tttcatcagc tgatgtgcaa tcaatcctgt gacccaattg
     1561 atgctattaa ttggcaagtt cggttgaagg aaggtcctta ataaatgcgt gtttggcaat
     1621 tgcttgtagc atttccatta gtttagtttg tgatcaagaa aaaaatctat cattctccaa
     1681 attaaaattt gaaaatttat gtttaaccgg gttcccttga ttatcatatt attcaaccag
     1741 tcacaacctc gatcatcctc gccggatcaa gatgtcgatg tgagcgttca aaaacttcct
     1801 ctgccgacat ttttacaatg gcatccatca atggttgcaa tgatataagg tcaacttcct
     1861 cgaccaggca cgaacggaat gtatcagccg tggcaaatac tccatcaaaa gatgtgataa
     1921 gaactccgat gagaaatgcc cgaacacgct ccaattcagc cttggaaggt ggtttcttaa
     1981 ggacattatc cagctgtcta tatacctcat tgcgcaattc cggcaagttt ttccatgatg
     2041 actcggccga tatatagaaa attccatcat gaacatatgt atcgtgggta ctcaaaatat
     2101 tgtaagtcaa ccccttttgt tctcgaatta tccgactgag tcttgcattg aagaagcctc
     2161 ctagcaaatg attaaaaatg taaaaggcag ggaagtcctc atgatctctt ttaattgtcc
     2221 gctgtcctag tttaaaggca cattggttct ttgaaaaaga attatccaaa tatttttcag
     2281 cctcactttt ttcaaccaac cttgtgttat tctccggcgg cgagtgaagc ttaactgagc
     2341 tcaacctgtc agtaagttct tgctttgatc cgggcatcag ccttccggta atcatcacaa
     2401 agtcttctga tgttaaataa ttcgactcaa aatgtagtct aagcaagtca gtgtgcatat
     2461 ggtgcaccaa ctcctctgta ctgttgtacc cataaggatg ttcggtaccg aaaatctttt
     2521 cagtcagtat cttataggca gaaaagtctg catcgtccct ctcctggcgc aatctgctaa
     2581 tatgcttact tttaagtaga gataattcat tctccggaaa ggaaggtttt gatatcactt
     2641 ttagaaacat gtccagcaat ggatcaaaaa acctattcag acaatagaaa gaaaatgaac
     2701 aataatcaaa gcctgcctgg tgattgagtg aagaaccaaa gtaatcgaaa tgctcggcaa
     2761 tctgaaatcc ggaataacct tcacagcctt ccttgatcag gctatggcat aacactgaag
     2821 ccaaccggct tttttctgaa gaacgtcctg ccttaaagca ccattcaaat ttaacaacct
     2881 cttgtgcccc tccttccaga aaatatagct tcagcccgtt gttcaggaca actttctcaa
     2941 ctgaaggaat ggaaagaggc ttgggtttgt ggattctcag tcctttcatt tttgctctgc
     3001 tacgagcaca agttttcctt tgacatttgc caacttgtat ttgatggtcg ctttccctcc
     3061 cgttggacaa caagtaggat cgatctcatt ataaagcggt ataaatctta gcaattgctt
     3121 cccatcaatg gcgaaactat cgtggcccat atatttatcc cggtattggg ccggcaaatc
     3181 acccatggaa atggaatcaa gtccggatgt ttcagcttga taaccataga gattaccata
     3241 acttccactg ccttcaccct gattgaagat atatacctca tccttaccat caccattcaa
     3301 atcagctgta aaagcatcga tgatctcacc atcgatctta atctttttat tgatattgcc
     3361 tccatccctc tgactgatga taagattgat catcttgccc ccttcaaaat ccttagtctt
     3421 aatggaaata tgcagatttt cagtggcaag ttttttggaa aaaccaaagg aagtatccgg
     3481 agttgatgtt ttcacccctt gtaattccac cacatcggta ttcacttcat ccgtattgct
     3541 gtctctcttg caggcagcaa aagttaaaac catcagcccc aaaagaaagg tcttcctgct
     3601 gaacatatta agataaaaat tcatttaata tctttttaca aattttcatt ttttgattgt
     3661 ttcccactca ttcttaacct cattgtcaaa tgactttagc catataatac aattggaaag
     3721 aaacaaggtt tcaaaaatcg gctgcaaagt tacaaataat aatattagtt acaatataca
     3781 agaaaaatta gcacaaagat ggaatgataa attttagtcc ggaatatgac attttatttt
     3841 atacttttgc gcattcattt gagatgagat ttcatattac aggaacatat ttgctaacca
     3901 cgatactcct cactaccctg gcgggatgca gtaaggaaga aagttcagta ccaacctatc
     3961 ttagttttgg ccaggcaaat atcatttttc aacaaaatgc aggaagtaat caccaaaata
     4021 tagaagatta ctggttgatt attaatggcc aaacaatcgc tgtggcatca ccggatcgta
     4081 ccgtacctgt tattggcaac gggatacagg aaatcacggt tcagccgggg atcagagtga
     4141 atggtcaaag ggatgaagct caaatatatt ccttcctgga gccatataaa acaactgtgt
     4201 cattgtgcgc atgtgccgat accatccatt taattccgga attcaaatat aagcctactg
     4261 cattgtttag cttcatcgaa gattttgaga acagcaaccg gttttcggtt gatcttgatg
     4321 acaacatagc gacatcgtca acgactcaaa cacaaactgt agcagaaggt agctttagtc
     4381 ttgccttgaa tgtagatgct accaataaga tcaactcggt cacaacccag gatttttata
     4441 ctgatttcga atctaatgga cgtgatcttt ttctggaatt cgaatataaa agtgatttca
     4501 cctttctgat cggattgcaa gccaataccg gttccggaca ctttactgaa tatgctgccg
     4561 gtgcttatgc cacaccaaca tggaagaaaa tgtatgtcaa ttttaccgat ctcgccctgg
     4621 catccaagag tacgggtggg tacaggccgg tatttaccat caatcacgcc ggtggcacgc
     4681 aaggtggagg gaatttatac ttagacaaca ttaaactagt tcattattaa tcggttatga
     4741 acaatacgag aaaacgggac ttattggttt atggcatttg cgatgccttg atggcattca
     4801 ctgcctgggc tattttctcg tacaaatttg tgatcaaccg taactttgtt ttactggaaa
     4861 atccaacatt gcaagcttgg ggcactgcag gcttgattgt tgtgggtttc atcctgctat
     4921 acaccatttt tgacttctat accgatcttt accggcaatc aagactggca actcttacac
     4981 gtacgatatt tatttccctg cttggttgtc ttcttgtggt tttgctgatc agagatgttt
     5041 tcatggggaa tacgatttcc ttgtggttcc attttttcct ttggcatttt ttcctgacgg
     5101 cgctgagtag aatgattctg cttacccgtg ccagcagaag actcaaagca gggtccgtta
     5161 ccttcaatac catcatcatt ggaggggatg aaaacgcgct ggaactatat accgaaatta
     5221 aaagcagaca aaaaggatta ggttataagt tcataggttt tattgacagt aatggctcct
     5281 ccaggaatct gttagaaggc cacctgccat tactgggcaa gttggatcaa ttggcagcca
     5341 tcatcgtgca gcataaggta gaggaagtca tcatagctat tgaaactcag gaacatgaca
     5401 agctgcgaga tatcctcaac gttctgttcg atttcagttc taatatcctg attaaggtca
     5461 tccctagcat gtacgacata atgttaggca ctgtaaaaat gaaccatgta tatggcgcgg
     5521 ttctgataga aatagagcag gttatgatgc ctaagtggca ggtacttgtc aaacggggcc
     5581 tggacatcgt catcagcatc attgtcattg tactgctcag tcctatgtat ttgtatatat
     5641 atattcgggt aaaaatgagc agtccgggcc cggcaatatt cttacaggaa aggataggtc
     5701 taaaccatcg tcctttttct atctataaat tcaggtcgat gtacaccaat gccgaagaag
     5761 acggccccca actcagtaat gaaaatgacg cccgatgcac accatgggga gctgtcatgc
     5821 gaaaatggcg tcttgatgag cttccacagt tcttcaatgt cctcaagggc gaaatgtcca
     5881 tcgtcggccc acgacctgaa aggcgttttt atattgaaaa gattatggaa agggcacctc
     5941 actataagca tctgctaaaa gtcagaccgg gtatcacttc ctggggacag gtcaaatacg
     6001 gctacgcatc caatgtggaa cagatgatcc aaagactcaa attcgacatt ctatacattg
     6061 agaatatgtc tctggccctg gattttaaaa tcatgttcta taccttgctg gtactttttc
     6121 aggggaaagg gaaatagtca gtagagtgaa attggaaaat ttcttattca aaaactgaag
     6181 agaagagtcc actccgtaat tttaatttca caacgacttg tgatccggat ggacaaccat
     6241 aaaatttata aatcctatgg atgagttgta acggtgatac cgattgcggc ttgaaatgac
     6301 aaacggtcgg ttgcaacaaa aaaacggagc acaagatctc ccgaccctat gctccattat
     6361 tcattgcatt tcaataaaat aagaattatc ttcttcgatt ttctcctgcc atcttgccaa
     6421 tcaattcttt cttaaaggaa cgttctgcca tttccagttt cgcaacttta gctacaggaa
     6481 gaattttttg aaaacttacg tagtaatccc gcttgatttc ggctgcttta atttcattat
     6541 ccagcatagt gttgataaaa cttgtctttt cagcatcact catttgttct atcggcttgg
     6601 aaggtctttg gctctgattc aattgatgta ctttttgctt gtattcctta taaaccggcc
     6661 agaatttttc tgattcagcc ggagtcaatg ataactcgtt ggtgatatag gcaactctct
     6721 gtgcctgtat cctttccatt cctttacctt tctgtgcata aacagaaaaa gaggtaaata
     6781 aaatgcaaat cacaaaaaat gatagtttgg ttttcaattt aataaaattt aaaagttaag
     6841 aaatttagtc aataatatcg tcgagtgaaa tttcatctat cagaagatcg tattcttcat
     6901 cggaaagatt gtccggattg tttgatgtag ctgtcaaggt tggatcggtg tttgcaatca
     6961 tctccagatc gatgtccatc acattttcct ttaaatatgt agcaatatct tcattggaga
     7021 taccattcaa attgatttca tgtgccggga cactctgtgg tttgaaaatt ataaacccgg
     7081 ccaggcaagc agctgctacc cccataataa aaaccaactt ccgaatcttt actattcccg
     7141 gacttaaacc cttcccggac tctaagggca atatcctgcc gttaccttgt tcaagacgtg
     7201 cagatatctt catctgcacc tcatcaaaat acccatcagg tatcctgaag ttatcctttc
     7261 tcaaattttt tatatctttt tccatattct catttattta gttggctaaa ataagcctct
     7321 atttttttag ttgcatggta ataagtagct ttcaaagcac cttcacttac tcccaacaat
     7381 tcacttaaat cgctgtattt catctcatca aagtatctca ggttgaaaac ctctttctgc
     7441 ttactcggca ataatgcgac tgccttcatc aattcaatct gaagttcatc accatcgaaa
     7501 aacggatctg cctgtaacca catttcggtc aactccggct taacctctcc atcagcaccc
     7561 acctttttct gccttcttaa aaacgacaat gcctcattag tcgcaatccg gtacatccat
     7621 gtaaaaagtg acgatttatt tccaaactgg ccgatatggg tgaatatctt gatcatcgta
     7681 ttctgaaata catcatcggc attttcatga gtcaacacta tcttacgaat gacactgtac
     7741 agtcgctctc tatattgatg catcagcatc cgaaaccctt gttcctgccg ggccttatca
     7801 agtagcattt ctaccagctg ctcatcagtc attgtattct ctaatagatt tatcaactca
     7861 atctcgcttt ttgattgtca ctttttcttc agacgcacaa ccatttgatg cggatttctc
     7921 taaaaggttt aattattaaa aataaatgta agaaaatagg tgttaaaaca gagggcaaat
     7981 tcaatgataa cagcctttaa tccacaattg gaagaaaata aaaccgtaga agtcctcctc
     8041 atctaaaata aattagccca tattaataca gataaaagat acacaacacc caataacatg
     8101 tgtcatgatc tcttaaaatt tcttggctca acgatacacc aggaaaacga aaaggtttct
     8161 tgtatttcca gaccaattct tgcatagtag aagaattctt caatatccta aagattccta
     8221 ttaaattaat gtattacggg gttgctgctt tcaaagagtc ttgagcatct ctctcatttg
     8281 tctatggatg gcggctgcgg cttcattgat ttgtgcaaaa tctccggcat aaattacctg
     8341 acggctgtaa ttggccaaaa tgcccactgt atctgaagaa agatacggct ggatgtcttt
     8401 taactgcccg ccttgtgcgc caaatccggg gaccagaaaa aaatggtcag gtgcaatctc
     8461 cctgatccct ttcagttctg acagattgtt cgctccaacg acaaacatcg tattgtcgat
     8521 atcgccccat gcaaggcatt tcctcagtac atcgatataa agtggtgagt cattctcagt
     8581 gactaacgtc tgaaagtcac tgctaccggt attggatgtc ttgactagaa taatggccca
     8641 cttgtccgga tacttcaggt aaggactcac agaatcgtgg cccatgtaag gcgaaagtgt
     8701 gattgcgtca acatccaact ccttgaaaaa cgcctctgca tacatatttg cagtattgcc
     8761 tatgtcgcca cgctttgcat cggcaattac aaagtgtgaa ctgccaatat accgaattgt
     8821 cttttcaaga cttaaccaac ctgaagcccc atgagcctcg aaaaaagctg tgttaggctt
     8881 gtaggccacg caatggtctc ttgtagcatc aatgaccatt ttgttaaact caaataaaga
     8941 atcatctgca tctgaataag gtgcgggtat tctggacata tccggatcca atccaacaca
     9001 aagaaaactc cctttgcttc gtatgagttc ggttaattgc cgttggttca catcacattt
     9061 ttattcttga atcagagcgc gccttctact tctatactta tgatctcggg aacccgggtt
     9121 ttaaggacct gttccacccc acctttt
//
LOCUS       DAOQMC010000074         9144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2960_length_9144_cov_4.417868, whole genome shotgun sequence.
ACCESSION   DAOQMC010000074 DAOQMC010000000
VERSION     DAOQMC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_9144_cov_4.417868"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..290
                     /locus_tag="PKV31_04410"
     CDS             30..290
                     /locus_tag="PKV31_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92133.1"
                     /translation="MSTQLNTFDKSQPTLQVFPNPAHEAVSIKFPGNFQYELFNVNGF
                     TARNGTGNDELRLDVSGFTSGIYLLKVKSGVNTQNATIVILR"
     gene            359..3328
                     /locus_tag="PKV31_04415"
     CDS             359..3328
                     /locus_tag="PKV31_04415"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB92134.1"
                     /translation="MKKIILHLLLVAFSSFNLYSQSEYWQQKVDTRINVLLNDSTHFL
                     HGNIAISYQNNSPDRLDFIYMHLYPNAYRNVNTAFAKQQLEARSTNFFFSEKWQRGYI
                     DSLDFKVVSNGNTESAKIENTQNIDIIKLMLPHALAPGETITIKTPFRVKIPYVFSRM
                     GHNDQSYQISQWFPKPAVYDASGWHPMPYLDQGEFYSEYGDYEVSITLPDNYIVMGTG
                     NIQEATENEWLDRLSQVKVDSMYISQHKDKPKSSPNQKTITFRESNIHDFAWFADKNW
                     VVRKDTVMQPDGSGIVTAYSAFYPKHYDAWKNSVNSIKKTIRGYGSKVGIYPYETVKV
                     VEGSLKAGGGMEYPTVTVIEASNNANTVDKVIVHEVGHNWFYGILGSNERDYPWMDEG
                     INSFYEGKFTGAGNRLQIFDKMDENNMAYTALGASRKLYPATMKSTMYPTINYGVDIY
                     MKVPLYLNYLEAYLGVEEFDRNMHEYFRTWQFKHPQPLDFESLFKKNSTKDIQWFFDM
                     MNSGEMVDLAIVNTQKTGNLTQVTIRNNNSFALPVHVYAESGGLIAAQQWTEPFVGEK
                     VLAFDNIAEPARFYIAPSIPNGNLKRNSSERKTRLKPLVNVNFNEYKTIWYSPAIGYN
                     YYDGFMAGLLLHNVTLPENKVRFYIAPMYGFKSGNVVGSGVINYTTHLKNKTIDNLDF
                     YLYFKKFGYNRTFYEDRSNPSLDYFKIAPEMVINFTKPNLRSPASSSLSLKGYYIGEQ
                     QFIFDTIPGTEPKKGAYGSKYYGKITYRFVNNKVINPSNYTVEAQAGAAFVKLSAEGN
                     WKFNYTVPKKAFHLRAYAGKFIKLTDSYFDYYRYRLSNTYTGSNDYLYDYTFGGRHEQ
                     HGFWSHQVGIREGGFKFATPLANNAPGTNDDYLIALNMKTDIPIKGVPIRLYCDVSKY
                     GGPVDKEYSGFLYEAGVEVHLLDYITVHIPIFMSKEYKEYKKFNLGNNFFKAISFSHN
                     LNSINWVRPTDIIYNLF"
     gene            3338..4192
                     /gene="nadC"
                     /locus_tag="PKV31_04420"
     CDS             3338..4192
                     /gene="nadC"
                     /locus_tag="PKV31_04420"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNB92135.1"
                     /translation="MTDIALINDFITRGLQEDTRGFDHTSLACIPADDTSDAWILIKD
                     NGVLAGVELAKMILLHVDPHAKVDILIQDGTAVEYGDIALTVTANSRSLLMAERLLLN
                     VMQRMSGIATLSRRFASEVSDLPVKILDTRKTTPLLRYFEKWAVRIGGCNNYRDGLYD
                     WIMIKDNHIDAAGSITGAIRSVNEYLKTNNLDLGITVEVRDFEEIREVLAVGNVTRIM
                     FDNFGLADLSKAVELVGGRFETEASGGVRLNTVRSIALTGVNFISVGALTHSATSLDI
                     SLKFRKGS"
     gene            4271..4948
                     /gene="pgmB"
                     /locus_tag="PKV31_04425"
     CDS             4271..4948
                     /gene="pgmB"
                     /locus_tag="PKV31_04425"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HNB92136.1"
                     /translation="MKLKFNEVGFIFDLDGVIVDTAVLHYKAWRRLANSLGFDFSEVR
                     NEELKGVGRMDSLDKILSWGGITMTQEQKLQAAERKNEWYGELITHLGPKDALPGAIN
                     FLEKCADKGIAIALGSASKNAMPVLNSLEITDYFDIIVDGHSTTKNKPDPEVFLKGAS
                     GLNLVPSLCAVFEDAAVGIEAACLAGMTAIGIGKKENLPKANQVYCCLAEANMDTILD
                     LIALNHM"
     gene            4995..7313
                     /locus_tag="PKV31_04430"
     CDS             4995..7313
                     /locus_tag="PKV31_04430"
                     /inference="COORDINATES: protein motif:HMM:NF010380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HNB92137.1"
                     /translation="MKKYIEIHPWKIIETDFSLNDNMVTESLLAIGNGRMGQRAVFEE
                     TFTGETLRGSYLAGIYYPDKTRVGWWKNGYPEYFAKVLNAVDWLGLNIQLDSTIIDLG
                     KLQPLDYRSELDMEKGLLSRTFGLQLPNDVYVRIHSERFYSKTHPDSALLHYRIKVEK
                     GEVDFTLDSGLEADVRNQDTNYGEAFWENFKSEITGNSGIVTAQTKKTGFRIAASTAH
                     KVMVNGWAINGGLDTPSRSSINANFSTFLSQDDEVIIEKYVSITTSFFYPHDDLTIKS
                     LENLDEITQLGYDELRDKQVEAWRKSWDQNDIVIEGDDEAQQGIRFNIFQLNQTYTGE
                     HALLNIGPKGFTGEKYGGSTYWDTEAYCLPYYLSTAGKQVGNQLLQYRYNHLTKAIEN
                     AGKLGFKDGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAYAIYHYTEYTGDKDYVL
                     NYGIEVLIGIARFWKQRVNWSDARNKYVMLGVTGPNEYENNVNNNWYTNYIARWCLKY
                     TIEILEELKATDPAHYAEIIQKTVFRDEEINDWKEVVDNIYLPYDEDLKIFLQQDGYL
                     DKEQIMVKDLDRDSLPLNQKWSWDRILRSCFIKQADVLQGLYFFEDQFDTDMIRRNFE
                     FYEPRTVHESSLSPCVHSILAMRTGNYEKAYEMYLRTARLDLDNYNNDTEDGLHITSM
                     AGSWLSIVHGFGGLKVREGKLRFSPSLPGKWNAYSFRILFRQNALEVRVEEHEIRFRN
                     IKGDEIELMVYGEAITIGRESTLIYPRKTYDA"
     gene            7341..8726
                     /locus_tag="PKV31_04435"
     CDS             7341..8726
                     /locus_tag="PKV31_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNB92138.1"
                     /translation="MKKWAQIIWLFLLPCCLHAQQTTGNMANFGMAAPEWTLRANIYE
                     VNIRQYTPEGTLKAFSAHLPRLKAMGVDIFWLMPVFPIGEVNRKGSLGSYYSIQDYKA
                     INPAFGTMADFDSLVARIHDLGMYVILDWVANHTAWDHPWTRQHPDWYNRDVQGNILV
                     PADNNGNLTDWTDVADLNYNNSEMRKEMISEMLFWAKEHHVDGFRCDIAGFVPLNFWQ
                     QAKAELDKAGHFFMLAEDENPDFHTGAFHMTYAWGVHHKMVDVAKGKANAKDLAKTLS
                     DEQVKFAKDAYRMQFIDNHDENSWQGPVSSRFGPGYKAFAVLTYIIPGMPLIYSGQES
                     SLNKSLRFFEKDTIAFDGFYDADFYTRLNMLKHKQAALANGVFGGDFTIIPNSSPEEV
                     LCFVRKKGNSELISLFNLSGKEAKVKYSGDGKITEGRYTEYFSGNKADIQSTGRLSLS
                     PWEYKIYIRDL"
     gene            8747..>9144
                     /locus_tag="PKV31_04440"
     CDS             8747..>9144
                     /locus_tag="PKV31_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB92139.1"
                     /translation="MTLTKPRLSFFQIFNMSFGFLGIQMGFALQNANASRILQSFGAD
                     VHSLSWFWIIPPLMGLIVQPIIGHYSDKTWTRMGRRKPYFLAGALLSALGLILMPQAG
                     IFASIMPALWIGAGFLMIMDASFNIAMEPF"
BASE COUNT         2772 a         1994 c         2028 g         2350 t
ORIGIN      
        1 ataacgattt tttttattta tagttctaaa taagtacaca attaaatact tttgacaaga
       61 gccagccaac attacaagta tttcccaatc cggcgcatga ggcggtgtcc atcaagtttc
      121 ccgggaattt ccaatacgaa ttatttaatg tgaatggctt tactgcacga aatggaacag
      181 gcaatgacga actacgtctt gatgtatctg gctttacgtc cggcatctac ctactgaaag
      241 tcaaatccgg agtgaacaca cagaatgcaa ccatagtcat cttacgataa tgataatttc
      301 ttacgaccga caaattcatt catatttacg tattatgctt aaagagaaaa atttaccaat
      361 gaagaaaata attctccact tactactcgt tgcttttagt tcgttcaatc tatattcaca
      421 atccgagtat tggcaacaga aggtggacac ccggattaat gtattgctga atgattccac
      481 ccattttctg catggcaaca tcgccattag ttaccaaaac aacagcccag accgcctcga
      541 tttcatctat atgcacctct acccaaatgc ctacagaaat gtaaacactg catttgccaa
      601 acaacaactt gaagcccgat ctaccaactt tttcttctct gaaaaatggc aacgtggtta
      661 catcgacagc cttgatttta aagtcgtatc caatggcaat accgaatcag ccaaaatcga
      721 aaacacgcaa aacatcgaca tcatcaagct catgcttcca catgccttag ctccgggcga
      781 gactatcaca ataaaaacac cattccgtgt caagatccca tatgttttct cacggatggg
      841 gcacaatgat cagagctatc agatatccca gtggtttccc aaaccagcgg tatacgacgc
      901 ttccggctgg catccgatgc cctacctcga tcagggagaa ttctacagtg aatacgggga
      961 ttatgaagtc agcatcacac ttccggataa ttatattgtc atggggaccg gaaatattca
     1021 ggaagctaca gaaaacgaat ggctagaccg actatctcaa gtcaaagttg acagtatgta
     1081 tatcagtcag cataaggata agcccaaaag cagcccgaac caaaaaacga ttacattcag
     1141 ggaaagcaac atacatgact ttgcgtggtt tgcagacaaa aactgggtcg taaggaagga
     1201 tacggtaatg caaccggatg gttcaggtat cgtgaccgcg tattctgcct tctatccaaa
     1261 gcactacgac gcctggaaaa actctgttaa ttccatcaaa aaaaccatcc ggggctatgg
     1321 aagcaaggtg ggaatatatc cctacgaaac agtgaaagtc gtggaaggct cactcaaggc
     1381 aggtggagga atggaatatc cgaccgtaac cgtcatagag gcttccaaca atgcgaatac
     1441 ggtggataaa gtgatcgtgc atgaagtcgg ccataactgg ttctatggaa tcctcggatc
     1501 caatgaaagg gattatccct ggatggacga aggcatcaac agcttctatg agggaaaatt
     1561 caccggcgca ggcaatcgat tgcagatctt tgataaaatg gatgaaaaca acatggcata
     1621 tactgccctt ggcgcttccc gcaagctata tccggccaca atgaagagca caatgtaccc
     1681 gaccatcaat tatggagtgg acatatatat gaaagtgcct ttatacctca actatcttga
     1741 ggcatatctc ggagtggaag aatttgacag gaatatgcat gaatatttcc gcacatggca
     1801 attcaagcat ccccaaccgt tagatttcga atccttgttc aagaaaaatt ccaccaaaga
     1861 tatccaatgg tttttcgaca tgatgaactc aggtgaaatg gtggatctgg ctattgtcaa
     1921 tactcaaaaa acaggaaatt taactcaggt tactatcaga aacaacaatt cctttgctct
     1981 tccggtacat gtatacgcag agagtggtgg gctgattgcc gcacagcaat ggacagagcc
     2041 gtttgtaggt gaaaaagtcc tcgcatttga caatatcgcc gaaccggcca gattctatat
     2101 agccccctcc ataccgaatg gcaatctgaa acgaaattct tccgagcgaa agacacgatt
     2161 aaaaccattg gtcaacgtca atttcaacga atacaaaacc atctggtatt cacccgcgat
     2221 aggatataat tattatgatg gattcatggc cgggctgttg ctgcacaatg tcacccttcc
     2281 tgagaataaa gttcggttct atatcgcacc tatgtatgga tttaaaagtg gtaatgtcgt
     2341 ggggtcaggc gtaattaatt ataccacaca tttgaaaaac aagaccatag acaacctcga
     2401 tttttacctg tattttaaaa aattcggtta taacaggact ttttatgaag atagaagcaa
     2461 tccatcactg gattatttta aaatcgcccc tgaaatggtt atcaatttta ccaaaccgaa
     2521 tttgagaagc ccggctagtt catccttgtc cctcaaagga tattacatcg gagagcagca
     2581 atttattttc gatacaattc ccggaactga gcctaaaaaa ggtgcttatg gcagcaaata
     2641 ttatggaaaa attacctata gatttgtcaa taacaaggta atcaacccat ccaattatac
     2701 cgtagaagcg caagcaggag cagcatttgt caaactttcc gctgaaggaa actggaagtt
     2761 taattataca gtacccaaaa aagcattcca tctgagagcc tatgccggaa aatttattaa
     2821 gctgacagat tcgtattttg attattatcg gtaccgattg tctaatacat acaccggaag
     2881 caatgactat ctgtatgatt atacctttgg gggtcgacat gaacagcatg gattctggtc
     2941 tcatcaggta ggtatcagag aaggcggctt taagtttgcc actccattgg ccaataatgc
     3001 tccgggtaca aacgacgatt acctgatcgc tctcaacatg aagactgata tacctatcaa
     3061 aggtgtcccg ataaggttat attgcgatgt ttccaaatac ggcggcccgg tggataagga
     3121 atactccggc ttcctatacg aagcgggtgt agaggtgcat ctgctcgatt atataaccgt
     3181 gcatattcct atattcatga gcaaggaata taaggagtat aaaaaattca atcttggtaa
     3241 taatttcttc aaagccatta gcttctccca taatctgaat tctatcaatt gggtgagacc
     3301 gacagatatc atttataatt tattctaatc aagaaaaatg acagatatag cattgatcaa
     3361 cgatttcata acccgtggct tacaggaaga taccaggggg tttgaccata cttcattggc
     3421 atgtattccg gctgatgaca caagtgatgc ctggattctg atcaaagaca atggcgtgct
     3481 ggccggcgtc gaacttgcca aaatgattct gcttcatgtg gacccacatg ccaaggttga
     3541 tattctgata caagacggta ccgcagtcga atatggtgat atagcactta cggtaactgc
     3601 caactccaga tcattactca tggcggagcg gctcttgctc aatgtgatgc aacgcatgag
     3661 cggcatcgcc acgctcagtc gtaggtttgc ctctgaggtt agtgaccttc cagtaaagat
     3721 cctcgatacc agaaaaacga caccattgtt gaggtatttt gaaaaatggg cggtccgcat
     3781 cggtggatgt aacaactacc gcgatggttt gtacgactgg atcatgatca aggataatca
     3841 tatcgacgca gccggaagta taacaggcgc gattcgcagt gtaaatgaat atttgaagac
     3901 caataatctc gatcttggca ttaccgtgga ggtccgtgac tttgaagaaa tcagagaggt
     3961 gctggctgtc ggaaacgtga cacgcatcat gtttgacaac tttggactgg ccgacttatc
     4021 caaggcggtc gaactggtag gcggtcggtt cgagaccgaa gcttccggag gggtcaggct
     4081 taatacggta agaagcattg cattgaccgg tgtcaatttc atatcagtcg gtgccctcac
     4141 tcattcagcc accagtttgg atatcagttt gaagttccga aaaggatcct gattttttat
     4201 tgtgtacttt ttacacaata tgtatatata attcatacct tagcaaggcc acttgataat
     4261 aacggcctga atgaagctaa aatttaacga agtaggattt attttcgacc tggatggagt
     4321 aatagttgac acggcagtgc tccattataa ggcatggcgc aggctagcca atagcctggg
     4381 atttgacttt tctgaagtcc ggaatgagga attaaaaggc gtaggccgta tggattctct
     4441 cgacaaaatc ctctcctggg gcggaatcac catgacacaa gaacaaaaat tacaagccgc
     4501 cgaacggaaa aatgaatggt atggtgaatt gatcactcac ctgggtccga aagacgcctt
     4561 gcccggagca atcaatttct tagagaaatg tgcagacaaa ggaatcgcaa ttgctcttgg
     4621 ctctgcttca aaaaatgcca tgcctgtcct gaacagcctg gaaattacgg attatttcga
     4681 tatcatcgtc gatggacatt ccaccacaaa aaacaaacct gaccctgaag ttttcctaaa
     4741 aggagcatcc ggcctcaatc ttgtgccctc attatgcgcg gtattcgaag atgcagctgt
     4801 aggtattgag gcagcctgcc ttgcaggaat gacagctatc ggtatcggta aaaaagaaaa
     4861 tttaccaaaa gccaatcagg tgtactgttg tcttgcagaa gcaaatatgg acaccatcct
     4921 ggatttaatc gctctcaacc atatgtagtg tccgtttcac caaaattcca atcttattta
     4981 tgaatgaatt agcaatgaaa aaatatatag aaattcaccc ctggaagatt atagaaaccg
     5041 acttcagcct caacgacaat atggtcactg aatccctgct ggccataggt aatggcagaa
     5101 tgggccaacg agctgttttc gaggaaacat ttaccggtga gacattgcgt ggctcttatc
     5161 tggcaggcat atattatccc gacaaaaccc gggtaggctg gtggaagaat ggctaccctg
     5221 aatatttcgc caaggtattg aacgctgtgg actggctggg actcaacatt cagcttgata
     5281 gtacaattat tgacctgggc aagttgcagc ctctggatta cagaagtgag ctggatatgg
     5341 aaaaaggcct tttgtcaaga acgtttggcc tacaattgcc caatgatgta tatgtacgta
     5401 tccactctga acgattttat tccaagactc atccggattc tgccttgttg cattacagaa
     5461 ttaaggtaga aaaaggagag gtagatttca cgcttgactc aggattggaa gcagacgtca
     5521 ggaatcagga caccaactac ggagaagcgt tttgggaaaa ttttaagtct gaaatcacag
     5581 gcaattccgg cattgttact gcacaaacca agaaaacggg attcaggata gcagcatcaa
     5641 ctgcccataa ggtgatggtc aatggctggg ctatcaatgg aggtctcgac acgccctccc
     5701 gcagtagcat caatgctaat ttctccacct ttctatctca ggatgatgag gtgattatcg
     5761 aaaaatatgt atccattacc acttcgtttt tctatccgca tgatgatctt accatcaaat
     5821 ctcttgaaaa tctggatgaa atcacacaac ttggctatga tgaattgcgc gataagcaag
     5881 tagaggcatg gcgcaaaagt tgggatcaga atgatattgt gatagaagga gatgacgagg
     5941 ctcagcaggg cattcggttc aatattttcc agcttaatca gacatatacc ggagagcacg
     6001 ctcttctgaa tattggtccc aaaggcttta ccggtgaaaa atacgggggt agtacatact
     6061 gggataccga agcctattgc ctcccatatt atttgtcgac agcaggcaaa caggtgggca
     6121 atcaattgct ccaatacagg tataaccacc tgacaaaagc gatagaaaat gccgggaaat
     6181 taggatttaa ggatggagct gccctttatc ccatggtcac catgaacggt gaagaatgcc
     6241 ataatgaatg ggaaatcacg ttcgaagaaa tacaccgaaa tggtgccatt gcctatgcaa
     6301 tatatcacta tacagaatat accggcgaca aggactatgt cctgaattac ggtatcgaag
     6361 tgctcatcgg cattgcccga ttctggaaac aacgggtcaa ttggtctgat gcacgcaaca
     6421 aatatgtcat gctcggcgtg accggtccca acgaatatga aaacaacgtc aataataact
     6481 ggtacaccaa ttatattgcc aggtggtgcc tcaaatacac cattgaaatt cttgaagaac
     6541 tgaaggccac tgatcctgcc cattatgccg aaatcattca gaaaacagtc ttccgtgatg
     6601 aagaaatcaa tgactggaaa gaggtcgtgg acaatattta cctaccctat gatgaagatc
     6661 ttaagatatt tcttcaacag gacggttatc tcgacaagga acaaatcatg gtaaaagacc
     6721 ttgatcgtga cagtttacct ttgaatcaga aatggagctg ggatagaata ctccgatcct
     6781 gctttatcaa gcaggctgac gtattgcaag gcttgtattt cttcgaagat caattcgaca
     6841 cggacatgat ccgtcgcaac tttgaattct acgagccccg tacagtgcat gagtcatccc
     6901 tgtcaccttg tgtgcactca attctggcga tgcgcaccgg aaattatgaa aaggcctatg
     6961 aaatgtatct tcgaactgcg aggttggacc tcgacaatta caacaatgat acggaagacg
     7021 ggctgcacat tacctctatg gcgggctcct ggttatcaat tgttcacgga ttcggaggcc
     7081 tgaaggttcg ggaaggaaag cttcggttta gcccatcatt accgggaaaa tggaacgcct
     7141 attcattcag gatattattc aggcaaaatg ctctggaagt tagggttgag gagcatgaaa
     7201 tccgtttcag gaacattaag ggtgatgaga tcgaattgat ggtatatggc gaagcgataa
     7261 ccataggcag ggaaagtaca ttgatctatc cacggaaaac atacgacgca tagtagaaaa
     7321 accatttaca aaaaacagat atgaagaaat gggctcaaat aatttggttg tttttacttc
     7381 cctgctgcct tcatgcccaa caaacaacag gaaatatggc gaatttcggg atggccgcac
     7441 ctgaatggac cctgcgagcc aacatatatg aagtcaatat ccggcaatat actccggaag
     7501 gtaccctaaa agctttttct gcccatctgc cacgactcaa ggcaatggga gtagacatct
     7561 tttggttgat gcccgtcttt cccatcggcg aggtcaacag aaaagggagc cttggcagct
     7621 attattccat tcaggattac aaagcgatca acccggcctt tggcactatg gccgattttg
     7681 attcgctggt agcaaggatc catgacttgg gcatgtatgt cattctcgat tgggtcgcca
     7741 atcatacagc ctgggatcat ccttggacaa gacagcatcc ggattggtac aatcgggatg
     7801 tccaaggaaa tatcctcgtt cctgccgaca acaatggcaa tctgaccgat tggacggatg
     7861 ttgccgatct gaactataat aattccgaaa tgcgcaagga aatgatatcc gaaatgctgt
     7921 tttgggccaa ggaacatcat gtggatggat tcaggtgcga tatagccggt ttcgtcccac
     7981 tcaatttctg gcagcaggcc aaggcagagt tggataaagc tggccatttc ttcatgctgg
     8041 ctgaggatga aaatcccgac tttcacacag gggccttcca tatgacctat gcttggggcg
     8101 tgcatcacaa gatggtggat gtggccaagg gcaaggccaa tgcaaaagat cttgcaaaaa
     8161 ccttgtccga cgaacaggtt aaatttgcaa aggacgcgta caggatgcag tttatcgaca
     8221 atcatgatga aaacagctgg cagggaccgg tcagttcgcg gttcggtccg ggatacaagg
     8281 catttgctgt cctgacctat atcatccccg gcatgccact gatatattcc ggccaggaat
     8341 cctccctgaa taagtcactg cgattttttg aaaaagatac gatcgccttt gatggatttt
     8401 acgatgctga cttttatacc aggctcaata tgttgaaaca caagcaggcg gcgctggcca
     8461 acggcgtctt cggaggagat tttacgatca tccccaattc atcgcccgaa gaagtcttat
     8521 gctttgtgcg aaagaaaggc aacagtgaat tgatatcatt attcaacctg tccggcaaag
     8581 aagccaaagt aaaatattca ggggatggaa aaataacaga aggtagatac acggaatatt
     8641 tttccgggaa taaagcagac attcagtcca cgggaaggct cagccttagc ccttgggaat
     8701 acaagatata catcagggat ctctaacctt tttacaaaaa ataatcatga cactcaccaa
     8761 accacggctt tcgttcttcc agatattcaa catgagtttt ggctttctcg gcattcagat
     8821 gggctttgca ctacaaaatg ccaatgccag ccggatactt cagtccttcg gtgccgatgt
     8881 acacagcctt tcctggtttt ggatcatccc gcctttgatg ggtttgattg tccagccgat
     8941 catcggtcat tatagcgaca agacctggac gaggatgggc agacgaaagc cttacttcct
     9001 ggcaggtgct ttgctatccg cattaggact aatattgatg ccgcaagcgg gaatttttgc
     9061 ttccataatg cccgcacttt ggataggagc aggcttcctg atgatcatgg acgcctcttt
     9121 caatatagcc atggaacctt tcag
//
LOCUS       DAOQMC010000075         9093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_2981_length_9093_cov_5.436490, whole genome shotgun sequence.
ACCESSION   DAOQMC010000075 DAOQMC010000000
VERSION     DAOQMC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2981_length_9093_cov_5.436490"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1016)
                     /gene="truB"
                     /locus_tag="PKV31_04445"
     CDS             complement(279..1016)
                     /gene="truB"
                     /locus_tag="PKV31_04445"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNB92140.1"
                     /translation="MQKTTLDNLPRELVAYQEGVLLLIEKPLNWTSFDVVNRIRVALC
                     KYFGIKKLKVGHAGTLDPLATGLLIICIGKFTKRIDEIQAQEKRYSGIIRVGESTPTY
                     DLESEIDKIHPIDHVTPIMIEQVRKQFVGEIMQLPPIFSAIKKDGKRAYELARKGINA
                     GLEARPIVIHDLKFDDTSWPDLFFEVTCSKGTYIRSLAHDVGVALDSGAHLKSLHRAG
                     IGEYDNKDAIRLEDFLEVFGKRKVAIQ"
     gene            complement(1019..2098)
                     /locus_tag="PKV31_04450"
     CDS             complement(1019..2098)
                     /locus_tag="PKV31_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNB92141.1"
                     /translation="MKILRNIFIILNIVAAMLLALSYFSPVINPYEHWQFGILGLMYP
                     FMVFTNLAFAAFWLFRKKWWFLISMVTILVGWNSFNGIIGFKQPQDTKDKDHTIRIMS
                     YNIRALSNLVKYPTPSLKENREKFAFFIEENKLPHVLCVQESTIPNINWFKANLAYPY
                     TCRYPGPNSNTAILSVFPIEENGVVKFDRGNGDCVWADINFNGQIIRFYSIHLHSNTI
                     SVQADTLLTMHKLEGEETLKGVEGMFKKYKLATKVRYRQAMKIKNHAESSPYPVVLCG
                     DFNDTPQSFIYHNLATGLKDAFKERGIGFGTTWAGNIPGLKIDYILVDPGIAVLSHDI
                     YRRPFSDHYPVKATILLPEKAKTKK"
     gene            complement(2100..3005)
                     /locus_tag="PKV31_04455"
     CDS             complement(2100..3005)
                     /locus_tag="PKV31_04455"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB92142.1"
                     /translation="MFDSIIRDIRYELTRGNTITKLILVNIGVFIVFLLTKVISMAIT
                     GGDESLYGQILSWFALSGDFRKLIIHPWTIITYSLVHLSIWQILFNMLLLYWFGQIAG
                     DLVGDRRIFPIYLYGAIVGGLLAVSAGSLLPTFNGKDFLILGAMAPVMAIIVAAATLA
                     PDYSMRLILIGPVRIKFIVLIIIVFLVAGLSTKFAAGSSYAGLGGALTGYLFIIALRN
                     GYDPTGLFRKPSRVSIGIKRQSDKKSKIISIFDTIKKHETELRENHPDHTAKRLDLIL
                     EKIKKNGRSSLSQDELDFLDKMSKS"
     gene            complement(3054..3659)
                     /locus_tag="PKV31_04460"
     CDS             complement(3054..3659)
                     /locus_tag="PKV31_04460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB92143.1"
                     /translation="MIRITNVVKQLIILNVIVFIIVSFSPELKGMLSLFFPTSPNFRV
                     WQPLSHMFNHNDIGHIFFNMFGLYIFGSLLESVWGEGKFLRFYLFSGIGAFIMQWAFW
                     YFTAGPNLNYVSMLGASGAISGCLMGVAVLAPNMQVMLLFPPIPLKMKYLAIGYFCLD
                     LFMGLGGGGNVANFAHIGGALTGLAISFYWKSRGFLHRRGN"
     gene            complement(3656..5497)
                     /gene="mutL"
                     /locus_tag="PKV31_04465"
     CDS             complement(3656..5497)
                     /gene="mutL"
                     /locus_tag="PKV31_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNB92144.1"
                     /translation="MSDRIKLLPEVLANQIAAGEVVQRPASVVKELMENAIDAGATNI
                     SLILKDGGKMLISVIDNGVGMSPIDARMSFERHATSKLSSVKDLFAIRTMGFRGEALA
                     SIAAVAQVEMTTKRAEDEVATRIVNEGNKITTQEATQAPTGTTIAVRNLFFNIPARRA
                     FLKSTDVERRHAYEEFIRLALAHPDIRFQLVHNDEIIHFLEPSNLKLRVVNLFKNDID
                     KRLLSVEEETDKLKIFGVIGTPQQSRKTRGDQYLFVNHRFIKSPLLNHAITSAYDKII
                     PSDHFPFYILFLELDPATIDVNVHPTKQEIKFDDEHIIYTYLKAAVKHTLSYNLVAPT
                     LDFEKDEQLSRAWNNEARSPHIPPANPVNQRPGENYRIPQEFIKENVGRWKDLYENIQ
                     INPEPEPKSDQPLIFNSSINEEKSISEDEEMPSVTYLQVHRSYIIFQVKSGLMIIDQH
                     NAHHRILYEQFYTKTREAQVITQTNLFPTTLYFDVVKADLLKTILDQIGTIGFDIEIS
                     GTNEFIVHGYPSFLAEGLDLQGWFDELIDTLYQGVEVNFDMHHVMAMAYADRKALPRG
                     KELKQREISELVDQLFACTEPSYDARGNSCFGRCTLLELEKIIINKK"
     gene            5722..6375
                     /locus_tag="PKV31_04470"
     CDS             5722..6375
                     /locus_tag="PKV31_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92145.1"
                     /translation="MNKLILFLCTFLPLLAAAQVTESSKVMSQGSNNALTVSLTDISV
                     SDAEKVWKDFAKTFGGKIKYNKKEKEYFVDDASVPSVSTNTIDLYSKAEKVGTEVAYS
                     VWFDLGGGYLSSTSNAAMYSNAVSFMKDFLREVERFKINEQLKIEMKSLEKLNDNLKS
                     LTRDKEGYEKDIKKAEEKITESKAKIEQNIKDQASKQSEINAQQSKIDKIKSDLSKL"
     gene            6524..7519
                     /locus_tag="PKV31_04475"
     CDS             6524..7519
                     /locus_tag="PKV31_04475"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNB92146.1"
                     /translation="MDFTQEQISELLLTIANGKDGYRMLMQQSLEAIMRAERKEFNEV
                     HSDYGNGYRFSKICVHNGKMELMIPRSRHHNFYPLILALIKDQNKESEALAFELYKSG
                     LTTEQVGKLFSKLYDHHYSKSAISQMMKSARQDVFSWLERKGLDEIYPVVFIDATFWP
                     TRRENEVSNEAYYTILGVKQDTTREVLSLVNHPTEGASNWEDALIQLKQRGVKRIELV
                     VVDGLNGIEDVINRVFPEAKIQLCTVHLTRNIVSKVKPVDKLQVVKDLKEVLDPELPD
                     DNPSKGIIRFNEFTRKWQSKYPSFKQYLRPKYALYFTYLNYEKEIRRMIYTTNWI"
     gene            complement(7915..8733)
                     /locus_tag="PKV31_04480"
     CDS             complement(7915..8733)
                     /locus_tag="PKV31_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNB92147.1"
                     /translation="MMKMKRLLLAAIYLVFSISGVMATDAPGLFPPKPEPAVYVHDYA
                     QWLTGEQANTLEQQLRAMHDTTSSQIVVMIRSDIGDYDRVTYATELGNRWGIGRSEKD
                     NGVVMLIVTEGNQRGIFISPGYGLEGALTDLQATQIARDMVPYFRAGNYYSGIQYGVS
                     AVISAIHGEYTADSTSNDATGSGGAWVILTIFLGIIILMVIIALFGKYDTQDLSGTGT
                     FGSGSRRSSSGGWYSGGSWGGGSWGGGSSSSGGDWGGGSFGGGGFGGGGGGASW"
     gene            complement(8758..>9093)
                     /locus_tag="PKV31_04485"
     CDS             complement(8758..>9093)
                     /locus_tag="PKV31_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNB92148.1"
                     /translation="DSCNGNLHDRAIEVFVEHGMQHTLNRNAVLIYIAVRSRKIYVWG
                     DEGIHAKAGQGLWDQILADLTADFKDSMFEAGILRAIEAIGSKLAEFFPRDGTEGNNE
                     LPDDIIYGF"
BASE COUNT         2656 a         1937 c         1961 g         2539 t
ORIGIN      
        1 aatcgatatc aaagaatttt atcaattcat gaccaccatt tttcaatagg tctatttttt
       61 cgatggcgca atacttcttt tgatacaagt aatgcacaag ttgtgagtaa gcaccggatt
      121 aatggcgttg tagcatctgt tgtatcatca attcatcaat agagtaatga ttggttgtaa
      181 ataaacagtt tcaccgaaaa tgtgattgct tcattttgat gcattgtacc aatgtgctta
      241 aaacttatgg caacgattaa tccctatccc gccgggggct attgaatagc gacttttctt
      301 tttccgaata cctcaaggaa atcctccagt ctgattgcat ccttattgtc atactcgccg
      361 attccggcgc ggtgcaatga tttgagatga gcaccgctat ccagcgcaac acctacatca
      421 tgggccaacg atcggatata tgtccctttg gagcaggtaa cctcaaaaaa taaatccggc
      481 catgatgtat catcaaattt caaatcatgg atcacaatag gtctggcctc cagacctgca
      541 ttgataccct ttctcgccag ttcataagcc cgcttgccat ctttcttgat ggcactgaag
      601 atcggtggaa gctgcatgat ctctccgaca aattgttttc tgacctgttc tatcatgata
      661 ggggtgacat gatctattgg gtggatcttg tctatttcac tttccaggtc ataggtggga
      721 gtggattcgc caacgcggat gatacctgaa tatcgctttt cctgtgcctg aatctcatcg
      781 attcgtttgg tgaattttcc aatacatata atgagtaagc cggttgccag gggatccagt
      841 gtgcctgcat ggccaacctt cagttttttg atgccgaagt atttacacaa agccacccga
      901 atgcgattga ccacatcgaa tgaagtccag ttgaggggtt tttcgatgag taataaaaca
      961 ccttcctggt aagcaactaa ttcgcgtggc aggttgtcca gagtcgtttt ctgcataatt
     1021 attttttagt cttggctttt tcaggaagta aaatggtggc cttgacagga taatggtcag
     1081 aaaatggcct tctgtagata tcgtggctta ggacagcaat ccccggatca actaagatgt
     1141 agtctatttt gaggccgggt atgtttcctg cccaggtggt gccaaatcca ataccgcgtt
     1201 ccttgaaagc atctttcaag cctgtagcaa ggttgtgata tataaaggat tgtggtgtgt
     1261 cattgaagtc accgcacaac accacaggat agggtgaact ttcagcatgg ttctttatct
     1321 tcatcgcctg acggtatctg acttttgtgg caagtttata tttcttaaac atgccttcta
     1381 cacctttcaa ggtctcctcc ccctccagct tatgcatggt caatagtgta tctgcctgga
     1441 cactgatggt attggagtgt agatgtatgc tgtaaaaacg gatgatttga ccattgaaat
     1501 taatatcggc ccatacacag tctccattac cacgatcaaa ctttaccacg ccattctcct
     1561 caatgggaaa tacactcaat atggcagtat tgctgttggg acccggatat cgacaagtat
     1621 aaggatatgc caggttggcc ttaaaccagt tgatgttggg aatggtgctt tcctgaacac
     1681 aaaggacatg tggcagcttg ttttcctcaa tgaagaaggc aaatttttcc cggttttcct
     1741 tcagactcgg tgtcggatat tttaccagat tggataacgc cctgatgttg taggacataa
     1801 tacggatggt atggtcctta tccttggtgt cctgaggttg cttaaaacct atgatgccgt
     1861 tgaaagagtt ccagccgaca agaatggtaa ccatcgatat gagaaaccac catttctttc
     1921 taaacagcca gaatgcagca aaggcaagat tggtaaacac catgaatgga tacatcaggc
     1981 cgaggatgcc aaattgccag tgttcgtaag gattgatgac tggagagaaa taggaaagtg
     2041 cgagcagcat cgccgccact atatttagaa tgatgaatat gtttctgagt attttcaccc
     2101 tatgatttac tcattttgtc cagaaagtcc agctcatcct ggcttaggct ggatcggcca
     2161 tttttcttta ttttctcaag aatcagatcc agtctttttg cggtatgatc aggatgattc
     2221 tctctcaatt ctgtttcgtg tttttttatt gtatcaaaga tgcttataat tttagacttt
     2281 ttgtcagatt gacgcttaat tcctatgctc acacgacttg gctttctgaa caatccggta
     2341 ggatcataac cgtttcgtaa ggcaataatg aaaagatatc cggtcaaggc acctcccagg
     2401 ccggcatagg aactgcctgc tgcaaatttt gtcgaaaggc cggccacaag aaagactatg
     2461 ataatcagta cgataaattt tatcctgacc ggaccgatca gaatcaatct catggaataa
     2521 tccggagcca aagtggctgc tgcaacaata atcgccatga caggagccat tgctcccagt
     2581 ataaggaaat ctttaccatt gaaggtcggc agaagactac ctgccgaaac cgccagcaag
     2641 ccacccacga ttgcaccgta aaggtagatt gggaagatcc tccggtctcc gaccaaatcc
     2701 cctgctatct ggccaaacca atacaacaaa agcatattga acaggatctg ccagatgctt
     2761 aagtggacga ggctataggt gatgatggtc cacggatgta taatcaattt gcggaagtct
     2821 cccgaaaggg caaaccagga taagatttgg ccgtacaggc tttcgtcacc accggttatg
     2881 gccatgctga tcactttggt aagtaagaat actataaaca ctccgatgtt gaccaatatc
     2941 aacttagtaa tcgtgttgcc ccgagtcagt tcataccgga tatccctaat tattgaatca
     3001 aacatggatc gaaatggtta atggtcttga tcatccttga tattcctgat tttttaattg
     3061 ccacgtctgt gtagaaaccc tcttgacttc cagtaaaaac taatggcaag acctgtcagt
     3121 gctcctccta tatgagcaaa attggctaca tttcctcctc ctccaagtcc catgaaaagg
     3181 tccaggcaaa aatatccgat ggcaaggtat ttcatcttca atggaatggg cggaaacagg
     3241 agcatgacct gcatattggg ggccagtaca gcaacaccca tcagacatcc gcttatcgct
     3301 cctgatgctc cgagcatgct gacgtaattg agatttggac ctgccgtaaa gtaccaaaag
     3361 gcccactgca ttataaaggc accgattcca ctgaacaagt aaaaccgaag aaacttaccc
     3421 tctccccaca cactttccag cagggaacca aatatataca atccaaacat attgaaaaag
     3481 atatgcccga tgtcgttgtg attgaacata tggctcaaag gttgccagac tctgaaattg
     3541 ggcgatgtag gaaagaaaag gcttagcata ccctttagct ccggtgaaaa gctgacgatt
     3601 ataaatacga tgacattgag tatgatcagt tgcttgacaa cattcgttat tcgaatcatt
     3661 ttttatttat tataattttc tctaattcta gtaaggtaca tctgccaaaa cacgaattgc
     3721 cccgcgcatc ataagagggt tccgtacagg cgaaaagttg gtcaaccaac tcgcttattt
     3781 ctctttgttt caactccttt cctcttggaa gagctttgcg atcagcatag gccattgcca
     3841 taacatggtg catatcgaag ttgacctcca ccccttgata caaggtgtca atcaactcat
     3901 cgaaccagcc ttgcaaatcc aggccttcag caaggaagga tggatagcca tgcacaatga
     3961 attcattggt gccgcttatc tctatatcaa agccgatggt gccgatctga tccaaaattg
     4021 ttttaagcag atccgccttg acgacatcaa agtatagtgt tgttggaaaa agattagtct
     4081 gggtaatgac ctgggcctcc ctggtttttg tataaaactg ctcgtacagt atcctgtgat
     4141 gcgcattatg ctggtcgata atcatcaacc cggattttac ctgaaagatg atatatgacc
     4201 tgtggacttg aagataggtc acggaaggca tctcttcatc ttccgatatc gatttttcct
     4261 cattgatgct ggaattgaat ataagtggtt gatctgattt gggctctggt tccgggttga
     4321 tttggatgtt ttcgtacaaa tccttccaac gacccacatt ttccttgatg aattcctggg
     4381 gaattcggta attttccccg ggtcgctggt ttacagggtt ggccggtggt atatgtggtg
     4441 atcgggcttc attgttccag gcccgggaca gttgttcatc tttctcaaaa tccagcgtcg
     4501 gagccacaag gttgtaggac aaggtatgct tgacggctgc tttgagatag gtatagatga
     4561 tatgctcatc atcaaattta atctcttgtt tggtgggatg gacattgaca tcaatggtcg
     4621 ccggatccag ctccagaaag aggatgtaga acgggaaatg atccgatggt atgatcttgt
     4681 cataagctga agtaatcgca tgattgagaa ggggactctt gataaatcgg tgattgacaa
     4741 agagatactg atcgcctctg gtttttcgcg attgttgagg ggtgcctatg acaccgaata
     4801 tttttagttt atcggtttct tcctcaaccg agagcaggcg tttgtcgata tcgtttttga
     4861 aaagattgac tacacgcagt ttcagattgg acggttcaag aaagtgaatg atttcatcgt
     4921 tgtgtaccag ttgaaaccga atgtcgggat gtgcgagcgc caggcgaatg aattcctcgt
     4981 aggcgtgacg gcgttcgacg tcggtggact tgagaaatgc ccttctggcc ggaatattga
     5041 agaacaggtt acgcaccgca atggtggtgc cggttggggc ctgagtcgct tcctgtgtgg
     5101 taatcttatt gccttcattg acgatgcgtg tggccacttc atcctctgcc cttttggtag
     5161 tcatctccac ctgggccact gctgcaatgg aggccagtgc ctcaccacgg aatcccatgg
     5221 ttcggatggc aaacaggtct ttgacactgg acaatttaga ggtggcatgt cgttcgaagc
     5281 tcatccttgc atcaatcgga gacataccca caccattgtc gatcacggaa atcaacattt
     5341 ttccaccatc tttcagaatg agactgatat tggtcgcacc ggcgtcgatg gcattttcca
     5401 tcaactcctt gactacggag gcagggcgct gcactacttc tccggcggca atctggttgg
     5461 ccaatacttc aggcaataat ttgattcgat ccgacatggg tatcaattaa ttgatgggtc
     5521 aaaggtagtc aacatcaact gtaaatttaa acgttggcgt tttgcaatgt aagattttat
     5581 aaaatgaata ttgctaaaac aatggaaaaa gataatgaaa acaataattt tgtaaatatt
     5641 gtttcaaaag ttgaacatag gttttgttac ctttttgatc aacaaaggtc taaatattta
     5701 taatgataaa aaccagatga catgaataaa ttaattcttt ttttatgtac atttctaccc
     5761 ttgctggcag ctgctcaggt aaccgaaagc agtaaagtga tgtctcaggg aagcaataat
     5821 gccttgacgg tttccttgac tgatatcagt gtttcggatg cagaaaaagt ttggaaggac
     5881 tttgcaaaaa cattcggcgg aaaaatcaaa tacaacaaga aggaaaaaga gtactttgtc
     5941 gatgatgcaa gcgtaccttc tgtaagtacc aacaccattg atctctattc aaaagccgag
     6001 aaggtaggaa ctgaggtggc atatagtgtg tggttcgatc tgggtggagg ctatctttcc
     6061 tctacctcca atgctgccat gtattccaat gcagtatcct tcatgaagga tttcctgcgg
     6121 gaggtagaac gcttcaagat caatgaacag ctcaagattg aaatgaaatc acttgagaag
     6181 ctgaacgata atctgaagag cttgactcgc gataaggaag gatatgaaaa ggatattaag
     6241 aaagctgaag aaaaaataac cgagtctaag gctaagatcg aacaaaacat aaaagaccag
     6301 gcaagtaagc aatcagaaat aaatgctcag cagtcgaaaa tcgataagat caaatccgac
     6361 ttgagcaaac tgtaaatctc aattataatc tgccatgcca ctggcctggc gaactgaagg
     6421 gagcataatg ccggatggat attttatgct cgatactgtt taaaaaagtg tgtaaagcca
     6481 aaaattaaga tttttgtaga taacaaaaca aaatcagaaa aaaatggact ttacacaaga
     6541 acaaatttcg gagctattat tgacaatagc aaatggaaaa gatggatatc gtatgttaat
     6601 gcaacaatca ttagaagcaa ttatgcgagc ggaacggaaa gaatttaatg aggtacattc
     6661 tgactatggc aacggatacc gattcagtaa gatatgcgta cacaatggta agatggaatt
     6721 gatgattccc aggagtcgac atcacaattt ttatccattg atattggcgc tgataaagga
     6781 tcagaataaa gagagcgagg cattagcatt tgagctatac aagtcaggat taactacaga
     6841 gcaagtaggt aagttgtttt ccaaactata tgatcatcat tatagtaaat cggcgattag
     6901 tcagatgatg aaatcggcca gacaagatgt atttagttgg ttagagagga aaggcctgga
     6961 cgaaatatat cctgtggtat ttatagatgc cacattttgg ccaacacgta gagagaatga
     7021 agtgagtaat gaagcttatt acactatatt aggcgtaaag caggatacaa cacgagaagt
     7081 tctgagtctg gtcaaccacc caactgaagg ggcatctaat tgggaagatg ccttaattca
     7141 actaaaacag aggggagtaa aaagaataga attggtagtt gtggatggat taaatggcat
     7201 agaggatgtt attaaccggg tatttccgga agctaagata cagttgtgca cagtgcatct
     7261 gacaagaaac attgtatcca aagtgaaacc ggtagacaaa ctacaggtag taaaagattt
     7321 aaaagaggtc ttagatccgg agcttccaga cgataaccca tccaaaggaa taatcagatt
     7381 taatgaattt acaagaaaat ggcagtctaa atatccatca ttcaagcaat atctaagacc
     7441 aaagtatgca ctctatttca cctatttgaa ttatgagaaa gaaataagaa gaatgattta
     7501 taccacaaac tggatttagc gattaaaccg aaattataaa agaacactga gaatgagaag
     7561 ctcaatgcca aatccggaat ctgttctttt tttattaggt agcgtagcga tggacagacc
     7621 agaatttaac taccctattt accaatttaa attaactaaa aaactgagtt ttataaatac
     7681 ttaattttaa acatgaatta caggattctt ttttttgggc tccttgaatt tttcataatt
     7741 aaaaaaaata cacacttttt tgaacattac cttatgctcc tttcttttac ttttccggga
     7801 atgaatattg ggcaaatagt ttatgagaaa tgatggactg gtttaattct ttttgataat
     7861 caacccgtct tccggagata gtctcatcgt ataagtgttt aaagataatc cgggtcacca
     7921 gctggctcca ccgcctccac cgccaaagcc tccgccacca aaacttcctc ctccccagtc
     7981 ccctccggaa gaggagctgc cacctcccca actgccacct ccccagctgc caccactata
     8041 ccatccacct gaggagctcc ttcgtgaacc actcccaaat gtaccggtac ctgataggtc
     8101 ctgggtatca tacttgccaa ataaagctat aatgaccatc aggatgatga tacctaaaaa
     8161 aattgtcagg ataacccagg cacctcctga gccggttgca tcgttggatg tactgtcagc
     8221 cgtgtattct ccatgtatgg cagaaatgac ggcagatacg ccatactgta tacccgaata
     8281 ataattgccc gctctgaagt agggcaccat atcccttgca atttgagtag cctgaaggtc
     8341 agttaaggct ccttcaagac cataacccgg tgaaatgaag atgccgcgtt gattgccttc
     8401 cgtgacgata agcatcacta cgccgttatc cttctcgctg cgaccgattc cccagcgatt
     8461 tcccagctcc gtagcatagg tcacacggtc ataatcgccg atatctgatc tgatcatcac
     8521 cacaatctgt gaagaggtag tatcgtgcat ggcgcggagc tgttgttcca gagtattggc
     8581 ttgctctcct gtcaaccatt gtgcataatc atgcacataa accgccggtt ccggcttggg
     8641 tggaaacaag cccggagcat cggttgccat aacgcccgaa atggaaaata cgagatatat
     8701 ggcagctaaa agtaatcttt tcattttcat catcctagac atcattactg atatggttca
     8761 aaatccgtag ataatatcgt ccggcaattc attattaccc tccgtgccat cccggggaaa
     8821 aaactcggca agtttactgc cgatcgcctc aatggccctt aagatgcctg cctcaaacat
     8881 gctgtcctta aagtcagctg tcagatccgc caaaatttga tcccaaagac cctgtccggc
     8941 tttggcatgg atcccttcat cgccccagac ataaatcttc ctggaccgca ccgctatgta
     9001 tatcagcacc gcattcctgt ttaaggtatg ctgcatacca tgctcaacaa agacctctat
     9061 ggcgcggtcg tgaaggtttc cattacagct atc
//
LOCUS       DAOQMC010000076         8979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3036_length_8979_cov_4.501457, whole genome shotgun sequence.
ACCESSION   DAOQMC010000076 DAOQMC010000000
VERSION     DAOQMC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3036_length_8979_cov_4.501457"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="PKV31_04490"
     CDS             complement(<1..1815)
                     /locus_tag="PKV31_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNB92149.1"
                     /translation="MFRRFTSYLHIAFMSILSYGALGQNVDTVDFVYFEEAKHGLIYT
                     KEKVSIFIGEVSFNHKGVYFYSDTVYRSDQLIRAIGNVLIQQGDTLSIFADSLHYNAT
                     TKQCELFGNVSFTKGEQRLYTEKLSYNTETKIAAFDEYGTFTNGPTWVKSKSAVYDTR
                     SEDIVLNEHVFVQDPDFDIKTSKLKYNSRTRLVTFLAPTLVAQKDGTRLYCESGFYNI
                     DKKYGEFVGNPQYQKGKDKAMARKKMIYDGRNEYYFMHGDARYQDSLRYVQGESVKYF
                     QKREYFEVDGGDGFAFLQDSSQMLKGKKIQYDKKKKILKTTGRSRIEDKDNIIEADNN
                     DFNDETGTGMSRGNVILHDKANNSTIHAECVDIDRKKENFKAFGKRAMFTVVNEEDTV
                     HISADTLISFKKDSLTGDTSRIILAYYHVKIFSKSFQGVSDSMYYTTLDSIFRLYRNP
                     VLWSDTTNQYFGDTILIFQKDKKLSSIMLQNKAMIITTPEEVYFNQISGKEIRAIMAD
                     DKIHALTVDGNARTVFYNRNDQEEYTGVNTLDCSHLRAVFDDGELSSIKFYKKNDGKY
                     YPMNKANHAEIQLSGFKWDISRRPVSLQEMRADGANVMM"
     gene            complement(1823..2950)
                     /locus_tag="PKV31_04495"
     CDS             complement(1823..2950)
                     /locus_tag="PKV31_04495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB92150.1"
                     /translation="MINRSYWEDISFFREPDFLIIGAGFVGLYTAYFLKKARPDSDVV
                     VVERGPFSNGASTKNAGFACFGSASEIWNDVAAYGRQKVVDTVRMRFEGLEMTKRILG
                     SQSIDYVECGGVDVFTEGDHAVYEDCVDNLLEINALAEEATGIPDIYSAHTGNPIGCK
                     GFTHFIRNRAEGSLNTASLYTSLLDLCRQEGVRLLFGLNVVNIDTDFRTVEFDFFDKI
                     SCPNIIVTTNGFASRLLPVLDVQFVRNQVLVTERIPGLDLLECVHYNSGFIYFRQVDG
                     RILMGGGRNVLGSAEVTDEMSTTNEAYELLRHVMINHIAVDTIPQVEYHWAGILGVGS
                     DKKPIIEQINPGIFVGVRMGGMGVAIAPVVGRELAELVVNS"
     gene            complement(2947..3285)
                     /locus_tag="PKV31_04500"
     CDS             complement(2947..3285)
                     /locus_tag="PKV31_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92151.1"
                     /translation="MRTQIPEIEAIIATGVIEAFVQFGPNESDRLTYKVKFDDSSALN
                     IEFHDREIIMHVPEATGRQWAESDEVGVYGSQDLGDGKTLRILLEKDFKCLDNTDEDQ
                     SMMYPNPRQA"
     gene            complement(3345..5003)
                     /locus_tag="PKV31_04505"
     CDS             complement(3345..5003)
                     /locus_tag="PKV31_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNB92152.1"
                     /translation="MSEEKNESLNFLEEIIEKDIAEGTHGGRVLTRFPPEPNGYLHIG
                     HAKSICLNFGLAEKYGGKTNLRFDDTNPVTEDVEYVNSIKEDIRWLGFEWANELYTSD
                     YFPTLYSYAIDLIKKGLAYVDDSTSEEMAALKGSPTVPGQPSPYRDRSIEENLRLFEE
                     MKEGKYGDGTKVLRARIDMASPNMLMRDPIIYRIKHAHHHNTGEQWCIYPMYDFAHGQ
                     SDSIEQITHSICTLEFIHHRPLYDWFIEKLEIFPSHQYEFARLNLNYTVMSKRRLLQL
                     VNEGYVSGWDDPRMPTISGLRRRGYTPASIRDFATRVGIAKRENVIDYGLLEFCIRED
                     LNKTAIRRMAVLDPLKVTITNLPDDFREALATDNNPEDESAGSRELSFSRQLFIEHED
                     FMAEPIPGFFRLTPGGLVRLRNAYIIRCDDMIRDEDGRVIELKCTYFPESKSGQDTSG
                     LKVKTAIHWVDANDSIDIEVRLYDRLFKVENPQNEEGDFKSYLSEDSLKILPNAKGER
                     ALIDTKPGDHFQFLRLGYFCTDKDTISKPVFNRTVSLKDGFKKK"
     gene            complement(5286..8582)
                     /locus_tag="PKV31_04510"
     CDS             complement(5286..8582)
                     /locus_tag="PKV31_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92153.1"
                     /translation="MKTIDNAVYRHSFCSLKTFNILQIFDVRLRTPFLLFLLISALVV
                     TSQAQTCPANQGTAVNIASLPYNGTGLTTCGAGNDFTSGNTTTCGSSTYLGGEDLVFL
                     FTPTVTGLISISLTSGSSWVGLKLYNGCPFNGQGGACVANAGSSSGNQSINTSVVAGV
                     TYYLIVDTWPSPTCIPSFNLTISAPTPPPTCPGGLGSYTNIIVPFSATGLTTCGKGND
                     LTSSNVSPICGSSSYLGGEDEVFVFTPSVSGSYTINLTSGSSYVGIMLYNGCPLSGNG
                     GTCVGYAQGSSGNQSITNILTAGVTYYLIVDSWPSPTCHSSYNLSIVLNGDPCSSITP
                     LACGTSTTANLSGAGVWSGGSSCGYGVPGQEKIYSFTPANTGTATLQVTSTNSGGYVD
                     YQYKLASGGCNSGGWTCIADVYSPGSYSFSVTGGQTYYLLLDAESYGSIIHTFQVNCP
                     VACVPSITVTETSGIVNDGTICVGGSATLSVGAGTSYLWSNGATTQSITVSPLLTTTY
                     SVTVTGGACPGTATKVVTVSPLPSASAGSNSPVCAGATISLTASGGTSYSWSGPGGWT
                     STAQNPTRPNATAAMAGSYTVTVTNAAGCSSMVTVDVTVNSLPSASAKADPNPVCTGH
                     FAQLAAAGGGTYSWSGPNGFASTLQNPGLGIVQMNMAGTYTVTVTSAEGCSSTASINL
                     VVNQSPNGSASVNPSSVCVGNTAQLSASGGQSYLWKGPGGFTSTQQNPVINVTSHVQG
                     GTYWVTISNSNGCYVQLSVTLTVNYPPAVTATHDQSTACTGSTLKLFGTGIGTYLWTG
                     PAGFTSSLQNPVIPNVTPANSGVYTLKVTNGSGCFAVASTTVSVVAPPNVTATVNDNT
                     VCEGTNVYLYSGGASTYQWTGPWGWVSTFEDPIIYTIPAYMSGVYSVTGTGPTGCKSS
                     ASVTVNVYQLINGTVNAIPNPVLLGGTLQLNATGGTSYLWTGPGGFYSTQSNPVIHNF
                     NYKNVGTYTVIITNEGGCEMTLFVTVDLVLPLQGDNVNNFTVSTTKLVEGHIYPNPSS
                     NYITLQRHETASVVYSIIDMKGNVLIKNATTDSGQVNIESLTPGSYHVLWSQPGVARD
                     AFKGSFIKVK"
BASE COUNT         2674 a         2034 c         1672 g         2599 t
ORIGIN      
        1 catcatgaca ttggccccat cagcccgcat ctcttgaagg gagaccggac gtcggctgat
       61 atcccattta aaaccggata attgaatttc agcatggttg gctttattca tgggataata
      121 ttttccgtca tttttcttgt agaatttgat agatgacaac tcaccatcat cgaaaaccgc
      181 acgcaaatgt gaacaatcca acgtatttac tccggtgtat tcttcctgat cattacgatt
      241 ataaaaaaca gttcgtgcat tgccatcaac tgtcagggca tgtatcttgt catccgccat
      301 aattgcccgg atttccttac cactgatttg attgaaataa acttcttcgg gcgttgtaat
      361 aatcattgcc ttgttttgca gcataatgga agacaatttc ttatctttct gaaagatgag
      421 gatggtgtct ccaaaatact gattggtagt atctgaccaa agtacgggat tcctgtatag
      481 tctgaaaatg ctgtccaacg tcgtataata catcgagtca ctcactccct gaaagctttt
      541 actaaatatc ttaacatggt agtaagccag aatgatcctg gatgtatcgc ctgtaagact
      601 atccttcttg aaggaaataa gagtgtctgc actgatatgc accgtatctt cttcattaac
      661 cacagtaaac atcgctcgtt tgccgaatgc cttaaaattc tccttcttcc tgtcaatatc
      721 cacacattcg gcatgtattg ttgagttatt cgccttatca tgcaagatca cattaccacg
      781 agacatccct gttcctgttt cgtcgttgaa gtcgttatta tcagcctcga tgatgttatc
      841 cttgtcctcg atacgactac gtccggtagt ttttaggatt ttcttctttt tatcatactg
      901 aatttttttt cccttgagca tctgactgga atcttgcaga aaagcgaagc catcacctcc
      961 atcgacttcg aaatattcgc gcttttgaaa atatttcacc gattcgccct ggacataacg
     1021 caaagagtct tgatatcgag catccccatg catgaaatag tattcatttc tgccatcata
     1081 gatcattttt ttccgggcca tggctttatc cttccctttc tgatattgtg gattcccgac
     1141 aaactcacca tactttttat cgatattgta aaaaccggac tcgcaataca atcgcgtccc
     1201 atccttttgg gcaacaagtg tgggagccaa aaaagtaacc agtctcgttc tggaattata
     1261 tttcaattta ctggttttga tgtcgaagtc aggatcctgg acaaaaacgt gttcattgag
     1321 aactatatct tccgacctgg tatcatagac ggcggattta cttttaaccc aggtagggcc
     1381 attggtgaat gtgccatatt cgtcaaaggc agcaatctta gtttctgtat tgtaggataa
     1441 cttttcagta tataagcgct gctccccttt ggtaaaagag acattaccaa acagctcaca
     1501 ttgtttagta gtagcattgt agtgaaggga atcggcaaat atgctcaagg tatcaccttg
     1561 ctggataagt acgttaccta tagcccgaat cagttgatcg gaacgatata ccgtgtcaga
     1621 ataaaaatat acacccttat ggttaaacga gacttcgcca ataaagatac tgaccttctc
     1681 tttggtataa atcaatccat gtttggcctc ttcaaaatag acaaaatcaa cggtatcgac
     1741 attttgaccc aatgcgccat aagacaaaat gctcatgaag gcaatatgga gatagctggt
     1801 aaaccttcta aacatcaaaa tcttaagaat ttacaactaa ttcagccagt tcacgaccaa
     1861 caacaggagc aattgcaacg cccataccac ccatacggac accgacgaag attccgggat
     1921 tgatttgttc aataattggt tttttatctg acccgacacc aagaatacct gcccagtgat
     1981 attccacttg aggtattgta tcaaccgcga tatgatttat cataacatgg cgcaacaatt
     2041 cataagcttc attggtcgta gacatctcgt ctgtcacttc ggcactaccg aggacatttc
     2101 gcccaccgcc cattaatatc cttccgtcaa cctgcctgaa ataaataaaa ccactattat
     2161 aatgaacaca ttctaacaga tcaagacccg ggattctttc cgttacaaga acctgattcc
     2221 gaacaaattg aacgtccaaa acaggcaaca accgtgacgc aaatccattg gtagtcacaa
     2281 tgatattcgg acaagaaatt ttatcaaaaa aatcaaactc aacggtccga aaatccgtat
     2341 caatattcac tacattaagg ccaaacaaca acctgacgcc ttcctgtctg caaagatcca
     2401 gcaaagaggt gtacaaggaa gctgtattga ggctaccttc cgccctattc cggataaaat
     2461 gtgtaaaacc cttacatcct ataggattgc cggtatgtgc agagtatata tcagggatgc
     2521 cggttgcctc ctcagctaaa gcatttattt ctaaaagatt gtccacacaa tcctcatata
     2581 ctgcatgatc gccttctgtg aacacgtcta ccccaccaca ttctacataa tcgatacttt
     2641 ggctgcccaa tatccttttg gtcatttcga ggccttcaaa tctcattcgc acggtatcca
     2701 caaccttttg acggccatat gcagccacat cattccatat ttccgaagca cttccgaaac
     2761 aggcaaaacc ggcatttttt gtactggctc cattgctgaa tgggcctcgc tctaccacga
     2821 caacatcgga gtccggacgc gctttcttca gaaaatatgc cgtataaagc ccgacaaaac
     2881 cggcacctat tatgagaaaa tcaggttctc taaagaagct gatgtcttcc caatagcttc
     2941 tgtttatcat gcctgacgcg ggttaggata catcatactt tggtcttcgt cggtattatc
     3001 cagacattta aagtctttct ccaaaagaat tcgaagagtc ttaccatcgc ccagatcctg
     3061 agatccgtat actcctactt catcgctttc cgcccattga cggcctgttg cttctggtac
     3121 atgcatgatg atttcacgat catgaaattc aatgttcaag gctgatgagt catcaaattt
     3181 taccttataa gtcagcctat cgctctcatt cggaccgaat tgtacgaagg cttcaatgac
     3241 acctgttgct atgatggcct ctatctcggg aatttgagtc ctcatacgaa tggagttttg
     3301 atacaacctt aatttcatgg caaaatctta taaatgatta aatcttactt cttcttaaat
     3361 ccatctttca gtgataccgt tctattaaac accggtttac tgattgtatc cttgtcagta
     3421 cagaaataac ccaatctaag gaattgaaaa tggtcacccg gtttagtatc tataagcgcc
     3481 ctttctcctt tggcattggg taatatttta agcgaatctt cactcaggta ggacttgaaa
     3541 tctccttctt cattttgcgg attctctacc ttgaacaatc ggtcataaag ccttacttca
     3601 atatcgatgc tatcattggc atctacccaa tgtatggcag tcttaacttt gagaccggat
     3661 gtgtcctgac cgcttttact ttccgggaaa taagtacatt tcagctcgat gacccttccg
     3721 tcctcatccc taatcatgtc atcacaacga atgatataag cattacgaag tcttaccaaa
     3781 ccaccgggtg tcagcctaaa aaaaccggga attggttcgg ccataaaatc ttcatgttcg
     3841 atgaaaagtt gtcttgaaaa actaagttcg cgactacctg cgctctcatc ttccggatta
     3901 ttatcagtgg caagtgcttc tctgaaatcg tccggcaaat ttgtaatggt aactttcaag
     3961 ggatccaaaa cagccatcct tcttatagca gtcttattca aatcttcgcg aatgcaaaat
     4021 tccaaaaggc cataatcgat tacattctcc ctcttcgcta tgcctaccct agtcgcaaaa
     4081 tcacgaatac tggccggcgt atatccacgc cttctcaaac ctgaaatagt tggcatccga
     4141 ggatcgtccc atccactgac atagccttca ttgaccaatt gcaacaatcg tcgcttactc
     4201 atcacggtat aattaaggtt gagtctggca aattcgtact gatgggaagg gaagatttcc
     4261 aatttctcta taaaccaatc ataaagtggt ctgtgatgaa taaattccag ggtacagatt
     4321 gaatgggtaa tctgttcgat gctatcactt tgcccgtgag caaagtcgta catcgggtat
     4381 atgcaccatt gttcacctgt attgtgatga tgagcgtgtt tgatccggta tataatcgga
     4441 tcgcgcatca acatatttgg ggaagccatg tctattctgg ctcttaaaac cttggttcca
     4501 tcgccatatt taccttcttt catctcctca aaaagtctca aattttcttc gatggagcga
     4561 tccctgtacg ggctgggttg gccgggcact gtcggacttc ctttcagggc agccatttct
     4621 tcagaagtag aatcatcgac ataggcaagg ccctttttga ttaaatcgat ggcataacta
     4681 tagagagtcg ggaagtaatc actcgtgtat aactcattag cccactcaaa accaagccac
     4741 ctgatatctt ctttgatgga gttgacgtat tccacatctt cagtcacagg attggtatca
     4801 tcgaaacgaa gattggtctt gccgccatac ttttcagcaa gtccaaaatt cagacatatc
     4861 gatttggcat ggccaatgtg aaggtagcca ttgggttcag gagggaatcg tgtcaacact
     4921 cggccaccgt gggtaccttc agcaatgtcc ttctcaatga tctcttccaa aaaatttaaa
     4981 ctttcattct tttcttcact catggattgt atctataatc aactaatcat caatactgca
     5041 gaatatcaac agcaaattag aaaacgcttt gtgtaaaaca ataaaacgga aatttcctaa
     5101 taatagtttg ccgtaaattt cattaataag tcggcaaaat tactctaaaa aatgaacaat
     5161 gccaacttgt attattccta ttcattacat tacaaaccta atgactttat ctcagaatta
     5221 tagatataaa taaaaaagag gcagtcaaca gactgcctct ttctagttac tttcacaatt
     5281 caaagttatt taaccttgat aaagctgcct ttgaatgcat ccctggcaac tcccggttga
     5341 gaccatagaa catgatatga tccgggagtg agtgactcta tattaacctg accgctatcc
     5401 gtagttgcat tcttgatcag aacattaccc ttcatatcga tgatggaata aaccaccgat
     5461 gcagtttcat gtctttgtaa tgttatataa ttactggaag gattaggata tatatgaccc
     5521 tctactagtt ttgttgtact gacggtgaag ttatttacat tatctccttg aagtggtaat
     5581 acaagatcaa cagttacaaa taatgtcatc tcacatccac cttcattggt aatgataacc
     5641 gtataagtac ctacattttt ataattaaag ttatgaatca caggatttga ttgagtagaa
     5701 tagaatccac ccgggcctgt ccatagataa ctggtacctc cggttgcatt aagttgtaat
     5761 gtcccaccta acaagacagg atttggtata gcatttacag taccattgat caactgatag
     5821 acattaacag tcactgaagc actggactta cagccggtag gacctgtacc tgtgactgaa
     5881 tagacgccac tcatataagc aggaatagta taaatgatag gatcctcaaa agttgaaacc
     5941 catccccaag gacctgtcca ctgataagtt gacgccccac ctgaatacaa gtaaacattg
     6001 gtaccttcgc aaactgtatt atcatttacc gttgccgtta catttggcgg agcaacaaca
     6061 gaaactgtgg ttgaagctac tgcaaaacaa ccggatccat tggttacctt cagggtataa
     6121 acaccactat tagcgggtgt cacattagga atgacaggat tttgcagtga actggtaaat
     6181 cctgctggac cggtccacaa ataagtacct attcctgtac caaacaattt caaagtactg
     6241 ccggtacatg cggtactctg atcatgagtt gctgtgacgg caggtggata attgactgta
     6301 agtgtgacgc tcaattgaac ataacatccg ttggagttag agatagttac ccagtatgtg
     6361 ccaccttgta catgtgaggt aacattgatt accggattct gctgagtaga agtaaatcct
     6421 cccggaccct tccataggta gctctgacca ccggaagcag ataattgagc agtattacct
     6481 acgcagactg aagacggatt cacacttgct gatccgttag gactttggtt gacgaccaaa
     6541 ttgatggaag ccgtggaaga gcagccctcc gcactggtaa cagtaactgt ataagtacct
     6601 gccatattca tttgaacgat gcccagtccg gggttttgta gtgtactggc aaatccattg
     6661 ggtccgctcc aactgtatgt accaccccct gctgcagcta attgtgcaaa atgacctgta
     6721 caaacagggt tcggatcagc ttttgcactt gcactcggca aactattgac tgtcacgtcc
     6781 actgtaacca tggaagaaca tccggctgcg tttgtaactg taaccgtata tgaccctgcc
     6841 atcgcagcag ttgcatttgg tctggtagga ttctgggcag tgctggtcca tcctccgggg
     6901 ccactccagc tgtatgatgt tcctccggag gcagtgaggc ttatggttgc acctgcacat
     6961 acaggactgt tggagcctgc tgatgcgctt ggcaatggac tgacagtaac cactttagtc
     7021 gctgtgccgg gacaagctcc tcctgtaaca gttacagagt atgtggtggt caagagaggg
     7081 cttacagtaa ttgattgtgt agtggctcca ttactccata aataagatgt accggcacct
     7141 actgataaag tagcacttcc tcctacacat atcgttccat catttactat acctgatgtt
     7201 tcggtgactg tgattgacgg cacacaagca accggacaat tcacctggaa agtatgtata
     7261 atactgccat aagattcagc atccaacaac aaataatacg tctgcccacc ggtaacacta
     7321 aatgaatatg aaccgggaga ataaacatca gctatgcacg tccatccacc ggaattacaa
     7381 ccaccggaag ccaatttgta ttgataatcc acataaccgc cactgttggt ggaggtaacc
     7441 tgcaatgttg ctgttccagt attggcgggt gtaaaggagt aaatcttttc ttggcccggc
     7501 acaccatacc cacatgaact accaccgctc catactccgg cgcctgataa attggccgtt
     7561 gtagaagttc cgcaagccaa aggagttata ctagaacaag ggtcaccatt tagtactatg
     7621 gaaaggttat aactggagtg acaagttggt gacggccacg aatcaactat caggtaatag
     7681 gttactcccg cagtcaatat attagttata gactgattgc ccgaggatcc ttgtgcataa
     7741 ccaacacaag tacctccatt tcctgatagt ggacaaccat tatataacat aataccaaca
     7801 tagctacttc cactagtcag gtttatcgtg taactacctg atacagaagg tgtgaaaaca
     7861 aatacttcat cttcaccgcc caggtatgaa gaactgccac agattggact gacattgctg
     7921 gaggttaagt cattaccttt accacatgta gtcaaccctg ttgcagaaaa aggcacaatg
     7981 atatttgtat atgagcccaa tcctccggga caagtcggag gcggtgtagg agctgagata
     8041 gtcaggttaa aactgggaat acatgtaggc gaaggccagg tatctactat caaataatat
     8101 gttactccgg caacaacact cgtatttatt gattgattac ccgaggaaga tccagcattt
     8161 gccacacaag ctcctccctg cccgttgaaa gggcaaccat tataaagctt caatccgacc
     8221 caagaagacc cgcttgttag cgaaattgat atcaaaccgg tgactgttgg agtaaacagg
     8281 aatacaagat cttcaccgcc caaataagtg ctgctaccac aagtagtagt attgccgctg
     8341 gtaaaatcat taccggcgcc acaagtggtt agccctgtac cattatatgg caaagaagca
     8401 atattgactg cagtcccctg atttgcgggg cacgtctggg cttgagaagt aacaaccaga
     8461 gcactaatca acaaaaaaag taagaaaggg gtacgtaaac gtacatcaaa aatttgtaaa
     8521 atgttaaatg tctttaaact acagaaagaa tgtctgtaaa cagcattgtc aatcgttttc
     8581 ataagattaa tttaagttat tagggaataa ttcactttgt tttcatcgtt tttatatgga
     8641 attataaatc tattgcaaaa gaaaatccat tatcccatat cgaaactaac aaaaacttca
     8701 tgacaaattt acaatattta atttaaatta taaaaccttc aataaataca ttttatttaa
     8761 atttttaaat gttaattaaa taaatccaaa taaacattaa tggatattat tattacaacg
     8821 caatgttcac atttaccata ttttatatgt tatattttca tataatcaca cttttaatca
     8881 tacaatatta tttttcaaaa aatataaaat tatttttcac ctgttttaac ccaagttgcg
     8941 cattattcaa caaattatgt taaaaaacgg tcaaagaac
//
LOCUS       DAOQMC010000077         8976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3037_length_8976_cov_5.559130, whole genome shotgun sequence.
ACCESSION   DAOQMC010000077 DAOQMC010000000
VERSION     DAOQMC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_8976_cov_5.559130"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1236
                     /locus_tag="PKV31_04515"
     CDS             130..1236
                     /locus_tag="PKV31_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB92154.1"
                     /translation="MKRFFSFVRKEFYHVFRDGKTMLMLFGMPVMQILLFGFALTNEI
                     KNSQIVICDYSRDDATRKIISKLEASNIFHIRESLLSHMQIEESFKSSNVKLVVIFPN
                     NFNHDLLHSNKAQVQIIADASDPNTATTLTNYVTSIIMTYQQQLMAINAFPMSITPEI
                     RMYYNPELKGATNFVPGVIALVLMLICVMMTSVSIVREKENGTMEILLVSPFNPMLVV
                     ISKAIPYLFLSIVNLLIILILSVSLLDMPINGSIFLLIAESILFIITGLSLGLLISNL
                     VESQQAAMLISMMGMMVPTILFTGFMFPIENMPIPLQIISYFIPSKWFYTIVKAIMIK
                     GLGFYDVWKETLVLAGMVVVLLTISFKKFKTRLA"
     gene            1233..2354
                     /locus_tag="PKV31_04520"
     CDS             1233..2354
                     /locus_tag="PKV31_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB92155.1"
                     /translation="MRTLKFLLQKEFKQIFRNRAILPLIFVVPVVQLLILPLAADYEV
                     KNINISVVDGDHSTNSRQLIAKITSSGYFRLTGYGTSFDEAYGLMESDDSDLILEIPA
                     GFERNLVREGEQKVFVAVNAINGVKASLGGAYLMRIISNYNDDIRLDWIQKPRFNPSP
                     QIEVTSSNWFNPHLDYNFFMVPAILVILVTMVGSYLCALNIVKEKEMGTIEQINVTPI
                     RKYQFVLGKLIPFWVIAMFVFTLGLFVVAYWVYGIVPLGSWVVLYSYLAVYLIAMLGL
                     GLLISTFSETQQQAMSISFFFMMIFMLMSGLFTPIDGMPEWAYVIARSNPVTYCIEVV
                     RMVVLKGSGFNDLKQHFLITAVFAVALNSFAIWNYKKTS"
     gene            complement(3144..3782)
                     /locus_tag="PKV31_04525"
     CDS             complement(3144..3782)
                     /locus_tag="PKV31_04525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNB92156.1"
                     /translation="MGKIKISAAEKADPLIHPFEAGSGIEAGVDEVGRGCGAGPVVAA
                     AVILPPKAKIPGLRDSKKLTASKRKELSGKIRELALDYSIAISTVEEIDQINILEATY
                     LAMTRAIAQLKIKPDLLVIDGNRFKNQTGINHRTVVGGDDKVLSIAAASVLAKTWRDA
                     YMEELHIEFPQYDWINNKGYLSTKHRQACFTHGLTPHHRKSFKGMTRQDDET"
     gene            complement(3770..6055)
                     /gene="bglX"
                     /locus_tag="PKV31_04530"
     CDS             complement(3770..6055)
                     /gene="bglX"
                     /locus_tag="PKV31_04530"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNB92157.1"
                     /translation="MTVLRSLYLLCFLLISQVSFGQKGKPDRMKVFIDELMSKMTLTE
                     KLGQLNLPVAGDITTGQASSSDIGKKIREGNVGGLFNIKSVEKIREVQRIAVEESRLK
                     IPLLFGMDVIHGYETVFPIPLGLSCSWDMDAIRRSARIAAIEASAAGINWTFSPMVDL
                     CRDPRWGRISEGNGEDPYLGSAIAQAMVRGYQGTLTENNQILACVKHFALYGAAEGGR
                     DYNTTDMSRIRMYNEYFPPYQAAVIAGVGSVMASFNEVDGIPATGNKWLLTDVLRKQW
                     GFKGFVVTDYTGINEMIDHGIGDLQTASAMALNAGIDMDMVGEGLLTTLPKSLKEGRV
                     TMAKIDAACRLILEAKYKLGLFEDPYKFCDEKRSKTEIFTPEHRAEARKIAAESFVLL
                     KNNGDLLPLRKSGTIALVGPLAHAQSNMTGTWSVAAVNDKSITLRQGLERALAGKATL
                     RYAKGSNLTEDAALEERGTMFGKSLDRDNRSDEELLREALEAAHSSDLIIAALGESAE
                     FSGESSSRTNLNIPDVQKKLLMELVKTGKPVVLVLFNGRPLTLTWEDAHVPAILDVWF
                     GGSEAGDAIADVLFGDVNPSGKLTTTFPQNVGQIPLYYNHKNTGRPLQEGHWFQKFRS
                     NYLDASNDPLYPFGFGLSYSKFRYSDIKVSSTSLKGNQKLTASVEITNVSDFNAKEVV
                     QLYIRDVVGSVTRPVKELKGFKKVEIGARQTVTVSFEITPEDLKFYNYDLQFDWESGD
                     FDIMIGANSRDVSKTRIYWEK"
     gene            6458..7465
                     /locus_tag="PKV31_04535"
     CDS             6458..7465
                     /locus_tag="PKV31_04535"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HNB92158.1"
                     /translation="MSVSKKFFCVDAHTCGNPVRLVAGGGPALIGANMSEKRQHFLSE
                     YDWIRKGLMFEPRGHDMMSGSILYPPHDPANDVAVLFIETSGCLPMCGHGTIGTITIA
                     IEEGLIRPREAGKVRMETPAGLVFVTYHQEGKKVKSVKLINVPAYLDSEGLEAECPDL
                     GMLRVDVAYGGNFYAIVDVQENFAGIEHYTADKLVAWARALRRNINERYSFVHPEDDT
                     IRGCSHILWTGKILDPNSTARNAVFYGDKAIDRSPCGTGTSARMAQWYTKGLLKPGDR
                     FVHESIIGSKFVGTIEEVTTVAGRPAIRPGIEGWARIYGYNTISIDDEDDPFAFGFQV
                     I"
     gene            7514..8761
                     /locus_tag="PKV31_04540"
     CDS             7514..8761
                     /locus_tag="PKV31_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB92159.1"
                     /translation="MKAVIIGGGIIGLSSAFFLKSSGWDVIVVDQGDMTHNCSYVNLG
                     MVVPSHFVPLAAPGMVSQGIRWMFDRKSPFYVRPTLSGRLISWGLKFMRSANDSHVSN
                     AAEPLRDLSLLSRSIFDTWAAMPELDFGYERKGIIMYFNSSKTGDEEVHLAEKAVKLG
                     LDAIVLGPQELSRLEPAVQPNVMGGVHYRCDGHLNPDRLMRQLMGLLADKGVLIERNR
                     EVIGFTQNHGRITSVKTKEGAMDTDLVVLANGSWLPDLAKMVGMNIPLMPGKGYSFMQ
                     ANPPAQLNIPAILCEARVAITPMGGGIRFGGTMEIDRIREGGVNMNRVAGIVESLPRY
                     FDNFRVEMPDMHQVSYGYRPCSPDGMPYIGSSRRHDNLLVAGGHAMMGLSLGPVTGKL
                     IAGLANGEKPEIDLKPFDPNRFD"
     gene            complement(8785..>8976)
                     /locus_tag="PKV31_04545"
     CDS             complement(8785..>8976)
                     /locus_tag="PKV31_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92160.1"
                     /translation="RFQKIVKNKFIEILQNIINKARNELSTLDSTLLNIYILHSKHPP
                     YQSSPGPISPAFYQPATPC"
BASE COUNT         2377 a         1760 c         2148 g         2691 t
ORIGIN      
        1 tagcgagagg agccaaaaga tcggtagatt gatgagaagc aagatgagtt aataatggga
       61 aacattcagt gaatttggat caaaagcggg aaaaataggg tcgtttgccc tacaaacaat
      121 agattgaaca tgaagagatt tttctcattt gtcaggaaag aattctatca cgtttttcgt
      181 gatggaaaga cgatgttgat gttgtttggg atgcctgtga tgcagattct actatttggt
      241 tttgcattga ccaatgaaat caagaattca caaatcgtca tctgcgacta ttcccgggat
      301 gatgcaacca ggaagatcat ctctaaactc gaagcgagca atatattcca tatccgggag
      361 tctctccttt cccatatgca gattgaggaa tcatttaagt cgtcaaatgt aaaactggtg
      421 gtaatcttcc cgaataattt taatcatgac ctgttgcatt ctaacaaagc tcaggtgcag
      481 atcatagccg atgcctccga tcccaacaca gctacaacgc tcaccaatta tgttacaagc
      541 atcataatga cttaccaaca acaattgatg gcgatcaatg cttttccgat gagtatcaca
      601 ccggagatca ggatgtatta caatccggaa ttgaagggag ccaccaactt cgtgcccggt
      661 gtcatagcat tggtgctgat gttgatttgt gtgatgatga cttcagtgtc cattgtgcgc
      721 gaaaaagaaa acggaacgat ggagatactg ctggtgtctc ctttcaatcc gatgctggta
      781 gtgatctcca aggccattcc ttacttattt ctttctattg taaatctcct gatcattctg
      841 attctgagtg taagtctgct ggatatgccg atcaatggaa gtatattctt actgatcgct
      901 gaaagcattc tgtttatcat aaccgggttg tcattaggat tgttgatttc caatttggtg
      961 gaatcgcaac aagcagcgat gttgatttcg atgatgggta tgatggtgcc gaccatctta
     1021 tttaccgggt ttatgtttcc aattgagaac atgcctattc cgttgcagat catcagttat
     1081 ttcattcctt cgaaatggtt ttataccatc gtcaaggcta tcatgataaa aggtcttggg
     1141 ttttatgacg tatggaagga aacactggtg ctggccggta tggttgtggt ccttttgacc
     1201 ataagtttca agaaatttaa aacacgtttg gcatgaggac attgaagttt ttactacaaa
     1261 aggaattcaa acagattttt cgcaacagag ctatattgcc attgatattt gtggttcctg
     1321 ttgtacaact tctcatcctc ccactcgctg cggattacga agtgaagaac atcaatattt
     1381 cagttgttga cggggatcat tcaaccaatt cccggcaatt gattgcgaag attacttcgt
     1441 ccggatattt caggctgacc gggtatggta cttcatttga cgaggcttac ggacttatgg
     1501 aaagcgatga ttcggattta atacttgaga ttcctgccgg atttgaacga aatttagtcc
     1561 gggagggaga acaaaaggtt ttcgtggcgg taaatgccat caacggtgta aaagcaagtc
     1621 tgggaggtgc ctatctcatg aggattatat cgaactataa cgatgacatc aggcttgact
     1681 ggatacagaa gccacggttc aatccatcgc ctcagatcga agtgacatct tccaattggt
     1741 ttaatcctca tttagattac aatttcttca tggtgcctgc catactcgta attctggtta
     1801 caatggtggg ttcgtacttg tgtgcgctaa atattgttaa agaaaaggag atgggtacaa
     1861 ttgagcaaat taacgtaaca ccgatcagaa aatatcagtt cgttttgggt aagctcatac
     1921 cattctgggt gattgcgatg ttcgtgttta cgctggggtt gtttgtcgtc gcctattggg
     1981 tctatgggat cgtccctctg ggaagttggg tggtgttgta tagttacctg gctgtctatc
     2041 tgattgcaat gttggggttg ggtttgttga tttctacatt ttcggaaaca caacagcagg
     2101 cgatgtcgat ctcgtttttc ttcatgatga tatttatgct gatgagtggt ctgtttacac
     2161 caatcgatgg tatgccggaa tgggcgtatg tcattgcccg cagtaatccg gtgacctatt
     2221 gtattgaagt ggtgagaatg gttgtattaa agggcagtgg gtttaatgat ttgaaacagc
     2281 atttcctgat aacggcagtt tttgcagttg ccctcaacag ctttgcgatt tggaactata
     2341 agaagacgag ttgattgaat gatatatccg gaataattga caatgcataa acttgtcaat
     2401 atgaaaatat ttaataccta atgtacattc gtaatagacc aatttcattg gcctaaacga
     2461 ctgtaacctc ggcattatac ctgataatta aggactatta tataaatcac cttggtataa
     2521 catcggcatc ccggaacccc ggattgttta actcccggga cagagcagag ctttagtagc
     2581 acagctatcc ggaccagtat acctgtaact accggattat tttataattc atccaggtat
     2641 aacctcgcgg gaaaacaaat attgataata aatggatatt ttggatatgt ctggtaatga
     2701 ttcaattttt ttattgtgtg aatttcaacg gttggtgctt taaaaaaagt gtatggtatt
     2761 gtattgcctg ttggattttc ggatactaaa tatttaaata gagaaataac gaaaaggtca
     2821 aattaaattc tttcatccta ctatgctcaa aagtgatatt atttgtattc aggcatatca
     2881 attcagagat atcgactgag gcaaatatta atgatctgaa tgaatggatc tgatttgaat
     2941 aaagcaatca aactttcatg ttaactttta taacagggta tcaaaacaag gaaactttgt
     3001 tcaagaagac cgtacaaact tcagtcagaa tgcgagggtg ctgagaattc ggttgctatt
     3061 aaggataaaa taaagcaata taaagtttta tttattttgg attacctttt tgttttattc
     3121 aggacgatca attcatcctt ttgtcatgtc tcatcgtcct gccttgtcat tcctttgaat
     3181 gactttctat ggtgaggggt aaggccgtgt gtgaaacaag cctgacggtg tttggtggac
     3241 agataccctt tgttattgat ccagtcatat tgtggaaatt cgatgtgtag ttcttccata
     3301 taggcatccc gccaggtctt ggccaatacc gatgccgcag caatgctaag aaccttatca
     3361 tcaccaccta caacagttcg gtggttgatt ccggtttgat tcttgaagcg atttccatct
     3421 atgaccaaca ggtcaggctt tatctttagt tgtgcaattg ctcttgtcat ggcgagataa
     3481 gtagcttcca gaatattgat ttggtctatc tcttcgactg tagaaatggc tattgaataa
     3541 tccaacgcca attcccgaat ttttccactt aattcctttc gtttagaagc cgtcaatttt
     3601 ttggaatcgc gtaatccagg gattttggcc tttggtggca gaataacggc tgcagccact
     3661 acgggcccgg cgccgcatcc gcgaccgact tcatctacac cggcttcgat acccgatccg
     3721 gcttcaaatg gatggatcag gggatctgcc ttctctgcgg ctgatatttt tatttttccc
     3781 aataaatcct tgttttgcta acatccctgc tgtttgcacc gatcatgatg tcaaaatcgc
     3841 cgctttccca atcaaattgc aggtcataat tgtaaaattt caaatcttca ggagtgattt
     3901 caaagctcac ggtaactgtc tggcgggctc ctatctccac ctttttgaag cctttcagct
     3961 ctttcacagg ccgggtgacg ctgccgacga catcccggat atagagctgg accacttcct
     4021 tggcattgaa atcacttaca ttggtgattt caacggaagc agtgagtttc tgattgcctt
     4081 tcaggcttgt tgatgaaacc ttgatgtctg aatatctgaa tttactataa ctcaatccaa
     4141 aaccaaacgg atatagcgga tcattgctgg catcgaggta attggatctg aatttctgga
     4201 accagtggcc ttcctggagt ggcctgcctg tgtttttatg gttgtagtag agtggtatct
     4261 ggcctacatt ctgagggaag gtggtggtca acttgccact tggattaaca tctccaaata
     4321 atacatcagc tatggcatca ccggcttctg atcctccaaa ccaaacatct aagatggccg
     4381 gcacatgggc atcttcccag gtcagcgtca gggggcgacc attgaataaa acgaggacga
     4441 ccggtttgcc tgttttgact aattccatga gcaatttttt ctgaacgtcc ggaatattga
     4501 gatttgttcg gctgctgctt tcaccactga attctgcgga ttctcccagt gcggctatga
     4561 tgaggtctga tgaatgtgcg gcttccaggg cttcccgaag caattcttca tccgagcgat
     4621 tatcccggtc taaggatttg ccaaacattg tgcctctttc ttctaatgcg gcatcttcag
     4681 ttaggttgga ccctttagca tagcgcagag tggctttacc tgctaaagcc ctttcaagac
     4741 cctggcgcag ggtaattgat ttgtcattga cggctgctac cgaccatgta ccggtcatgt
     4801 tgctttgagc atgagccagt ggaccgacga gggcgatggt accggatttt ctcagtggta
     4861 gtaagtctcc gttatttttc aagaggacga aactttctgc ggcgattttt ctggcttcag
     4921 cacgatgttc cggcgtgaaa atttcggttt tggatcgttt ttcatcgcag aatttataag
     4981 gatcttcgaa gaggccgagt ttgtattttg cttcaaggat taggcggcat gctgcatcga
     5041 ttttagccat ggtcactcta ccttccttga gtgactttgg cagggtagtc aacaggcctt
     5101 ctccaaccat gtccatatct atgccggcat tgagtgccat tgcactggct gtctgcaagt
     5161 ctcctatgcc gtggtcgatc atctcattga taccggtgta atctgtcacc acaaatccct
     5221 tgaaccccca ttgcttgcga aggacatccg tcagtaacca cttattaccg gtagcaggta
     5281 taccatccac ttcattgaac gaagccatca cgcttcctac gccggcaatg actgcagcct
     5341 ggtaaggagg aaaatattcg ttgtacatcc ggatgcggct catatcagtg gtattatagt
     5401 cccgtcctcc ttctgcggca ccgtataggg caaaatgctt gacgcatgcg aggatctgat
     5461 tgttttcggt caaggttccc tgataacctc ttaccatagc ctgagcaatt gcacttccca
     5521 ggtaaggatc ttcaccattg ccttccgata ttcttcccca tcgaggatcg cgacacaggt
     5581 ctaccatagg agagaaggtc cagttgattc cggcagcact tgcctcgatg gcagctatcc
     5641 gtgcacttct gcggatggca tccatatccc aactgcatga tagccccaga ggaatgggaa
     5701 acaccgtctc ataaccgtga ataacatcca taccgaacaa cagtggtatc ttcaaccggc
     5761 tttcttccac agcgattcgc tgaacctctc ggattttctc tactgatttg atattgaaca
     5821 ggccacctac gttgccctcc ctgatttttt taccgatatc cgagctggag gcctggcctg
     5881 tagtgatatc gcctgcgaca ggcaagttca actgacccag tttttcagtc aatgtcattt
     5941 tcgacatcaa ttcatcaata aagaccttca tcctgtcggg cttgcctttc tggccaaagg
     6001 aaacttgaga gatgagcaaa aagcaaagta gatacagact gcgcaatact gtcatggttt
     6061 gaatttttta aggataaata taggagattt cacatggaga ggtggaaata tttttgaaag
     6121 tataactgat cattgatgta aagatattgc tcatcaactt actattacta tttgatttag
     6181 ggaaatgtgt actttcatat ccctgatttt tggtcccttt ttaatagatt catttatgtc
     6241 aagctgaaag tcaaaaggag atgcccttgt ttttttaatt tgataaaggg acattattta
     6301 atgtacggta atgtcaataa ttacctgacg gattgatagt ccgaagattt ggggaggggt
     6361 agggaaatgc tggatggaaa tttataatca ggattgacaa ctatttattc aaattccctt
     6421 aagcctgtta tttttgttgt gaaatcaaga aagtaacatg tctgtttcaa agaaattctt
     6481 ttgtgtggac gcccatacct gtggtaatcc ggttcgcctg gttgcaggtg gagggcctgc
     6541 attgatcggg gccaatatga gtgaaaaaag gcagcatttc cttagcgagt atgattggat
     6601 tagaaaagga ctaatgtttg agccgcgggg tcatgatatg atgagcggga gtatccttta
     6661 tccgccgcat gatccggcaa atgatgtcgc agtacttttt attgagacaa gcggttgctt
     6721 gcctatgtgt ggccatggca ccatagggac tatcactatt gccatagaag agggtttaat
     6781 aaggcccagg gaagccggaa aggtcaggat ggagacacct gccggtctgg tattcgtgac
     6841 ttatcatcaa gaaggtaaaa aggtcaaaag tgtcaagttg ataaatgtgc ctgcttatct
     6901 agattcggaa gggttggaag cagaatgccc ggatctgggt atgttgagag ttgatgttgc
     6961 atatggcgga aatttttatg ctatcgtcga tgttcaagaa aactttgccg ggatcgagca
     7021 ttatactgca gacaagctgg tagcctgggc cagggcatta cggcgcaata tcaatgagag
     7081 atacagtttt gttcatcctg aagatgatac tatccgtggt tgctcacata tcctgtggac
     7141 aggaaaaatc ctcgacccaa actctacagc tcgcaatgca gtgttttacg gggataaagc
     7201 gatcgacaga agtccgtgcg gaaccgggac ttcggctcga atggcacaat ggtataccaa
     7261 gggcttactg aaacccggtg atcgttttgt acacgagagc atcatagggt cgaaatttgt
     7321 tggtacgata gaggaggtaa caacggttgc aggaaggcct gccatacggc ccggaatcga
     7381 aggttgggca cgtatatacg gctacaacac catcagtatt gatgacgagg atgatccatt
     7441 tgcttttgga tttcaagtaa tttaaccaat tgttataacg aattaggtca cctgttttaa
     7501 atagattgcg gaaatgaaag cagtaattat aggtgggggc attatcgggt tgagctcagc
     7561 atttttcctg aaaagcagtg gatgggatgt catagttgta gatcagggcg acatgacgca
     7621 caattgttcg tatgtaaatc ttgggatggt cgtcccgagt cattttgtgc cattggcagc
     7681 tccgggcatg gtgtcgcaag gcattcgatg gatgtttgac cggaagagtc ctttttacgt
     7741 caggccgact ctgagtggga gattgatttc atgggggtta aaatttatga gaagtgccaa
     7801 tgatagtcat gtgagtaatg ctgctgaacc tctgagagat ctgagtcttc tgagtcgcag
     7861 tattttcgac acatgggccg caatgccgga attagacttc ggatatgagc gcaaaggcat
     7921 aattatgtat ttcaattctt caaaaaccgg tgatgaagaa gtgcatttgg ctgaaaaagc
     7981 tgtcaaactt ggtttggatg cgattgttct agggccgcag gagcttagtc gacttgagcc
     8041 tgcagtccag cctaatgtca tggggggcgt acattatcgt tgtgatggtc atctgaatcc
     8101 ggacaggctc atgaggcagt tgatggggct attggccgat aaaggggtat tgatcgaaag
     8161 aaatagggaa gtaataggtt ttactcaaaa tcacgggagg atcacttcgg taaagaccaa
     8221 agaaggggcg atggatacgg atctggttgt cttggccaat ggttcatggt tgcctgacct
     8281 ggctaaaatg gtagggatga atattccttt gatgccgggc aaaggttatt ccttcatgca
     8341 agcaaacccg ccggcgcagc tgaatatacc tgctattctt tgtgaggcca gagtagccat
     8401 tacaccgatg ggagggggaa ttcgttttgg tgggacaatg gaaattgacc ggattagaga
     8461 gggtggtgtc aacatgaacc gtgttgcagg gattgtggaa tccttgccgc gatactttga
     8521 taacttccga gttgaaatgc ctgatatgca tcaggtttca tatggataca ggccttgttc
     8581 tccggatggg atgccttaca tcggttcatc tcgcaggcat gacaacttgc ttgttgcagg
     8641 tggccatgcc atgatggggt tgagtcttgg gcctgtaacg ggaaagctga ttgctggttt
     8701 ggccaatgga gaaaagcccg aaatagattt gaagcctttt gatccaaaca gatttgattg
     8761 agtaaaaata gcaatattgg taatttagca gggagtggct ggttggtaaa aggcaggtga
     8821 gattggacct ggagaggatt ggtaaggggg gtgttttgaa tgaagtatgt agatgtttaa
     8881 tagcgttgaa tcaagtgttg ataattcatt ccgggctttg ttaataatat tttgcaagat
     8941 ttcaataaat ttattcttga caattttttg aaacct
//
LOCUS       DAOQMC010000078         8976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3038_length_8976_cov_4.561484, whole genome shotgun sequence.
ACCESSION   DAOQMC010000078 DAOQMC010000000
VERSION     DAOQMC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_8976_cov_4.561484"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1294)
                     /locus_tag="PKV31_04550"
     CDS             complement(8..1294)
                     /locus_tag="PKV31_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92161.1"
                     /translation="MTNIKNLLFVFLACIPVLFTACDKDECIKTCAPNEVLTDNCECV
                     PVGDNSTVIVSGNISSDVTWTADKVYVLAQRVTVLPGVKLTIMPGTVIKGQAGSGANA
                     TALLIAKGAKIDAQGTVDKPIIFTSVADEIKSGEIVSPNLDQGINGLWGGVLILGDAP
                     ISANAPSVQIEGIPPSDINGLYGGNNPQDNSGIMKYVSIRHGGTNIGEGNEINGLTLA
                     GVGSGTIIENIEIVSNQDDGIEIFGGTVNPKNIMVWNTGDDMFDCDQGWSGTMDNIIG
                     IAGDQTDSALELDGGEGVENPTYTISNGTLKGHTAATSGKYADLRAKVSVSLKNLYFF
                     NFSEGSEFRLNDQGVSDSWFSGKILFANLEFNVSHMNTQPSLADIFRDKGSHDEDLNN
                     AAPGFAKISTANTVGARVSEFEKWTLVAALGQLAQL"
     gene            1805..1972
                     /locus_tag="PKV31_04555"
     CDS             1805..1972
                     /locus_tag="PKV31_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92162.1"
                     /translation="MVRMYGMGQFMDDYLVGSLKGCKEEPVGEYNVVLGYTGSPVRDI
                     ITEGVYPYLKF"
     gene            complement(2378..3238)
                     /locus_tag="PKV31_04560"
     CDS             complement(2378..3238)
                     /locus_tag="PKV31_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92163.1"
                     /translation="MKYILLYILCFGMVFGLHAQNTTWEQYDEELSTMGKIILTDTNS
                     TERKAAVEHVNDLFNTLLQDPATFKKKFVKSDFFSVLYPTDSSFRILTGQHFIKDDEY
                     RYYGGIQKASGGFIPFTDRSYENFSEGITNEELRADEWQGALYYRLFDCKLKGDKYYL
                     LLGYNAHSFFNKQKVIEVLSFDQDGRPLFGKDVFIRDSTDYGDDRLRYIYTYSADVAM
                     KLNYDNEEKMIVLDHLIPMREIFVGQGDTYVPDGTYEGFKYKRGRWRHISFLPQRFMK
                     ESEMKSGKKK"
     gene            complement(3426..4523)
                     /locus_tag="PKV31_04565"
     CDS             complement(3426..4523)
                     /locus_tag="PKV31_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB92164.1"
                     /translation="MFGWQFRKYDGEPELSLFDELLNLFKDLLMYAGGNVATAIQWLM
                     EMDREYNLTRDDYGIQDFLDELMQKGYLKADPEENALRPAEKMNISLRRKALKEVFGN
                     IKKATKGNHTTKKDGKGDELTSDLRPYVFGDSPEQIAFVESIKNAQINHGPGSFSLEY
                     EDLEIRETYHQSSMSSVLLIDISHSMILYGEDRITPAKKVAMALSEYISTRFPKDTLD
                     IVVFGDEAWKIEKDELPYLEVGPFHTNTLAALELALDILRKRKNTNKQVFMITDGKPT
                     CIKRGNKLYKNSFGLDRMIVNRTLNQAGKFRRLGIPITTFMIAKDPYLMEFVELFTET
                     NGGKAFYSDLQGLGDFVFKDYSRSRRWNNKW"
     gene            5104..5712
                     /locus_tag="PKV31_04570"
     CDS             5104..5712
                     /locus_tag="PKV31_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92165.1"
                     /translation="MVLMYQDHHTIQVIESDFGAFKIYLDDISPDTLYPRSSMEGMRI
                     KYTKSSKRIFINAPIQGITTTYIGKFRYRDSGDLERQKAAYDSIMRHKSKEVKTYGTY
                     YGTIFNSKTNKRQSDTIKVFDFKIDQTEGNFQVKYKMARIIFSGADSQPYEIRVEYNP
                     NNKTIRQSGKSSIKLFIDLENKVIRFEDKVKKIKFEGIRIEH"
     gene            5718..6659
                     /locus_tag="PKV31_04575"
     CDS             5718..6659
                     /locus_tag="PKV31_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92166.1"
                     /translation="MAGSVPYNIAILYYTQTGQLKMLAEKVSEGFSGDCRVDFIEIIP
                     VKQFPFPWNTIEFFDAMPECVLEEGSEIRLSGYEEGKHYDLVILAYQPWFLHPSLPIT
                     GFLQSKWADKLLKDVKVITLTGARNMWLNALEKIKRDLHKFGSEHVGNIAFVDSSPNL
                     ISTLTVIRWAFKGRKEASGLLPEAGVQVAEINRGPLFGKLIASALQEGKWQTLQQELV
                     KAGAVKLKPGLILLEETAIGQFKMWARKIKAKGERGSPARRPLVRLFKNILIVGIFIL
                     SPIKFVVSKIQAMLNRKRLVAAAKYFQSVNYEENKFS"
     gene            6675..7103
                     /locus_tag="PKV31_04580"
     CDS             6675..7103
                     /locus_tag="PKV31_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB92167.1"
                     /translation="MKKNEVPQDDEAIYQQKFGDGLLKYATDEQNEYVTVQSVGWEPE
                     IVALKQAWELVEEKVEMARQAVKNNQKSPIYYFMEKKLMDTGILAGYMGLWRWQVARH
                     FKPGVFARIKPSTLEKYAKVFDIDLKQLTDFDPDHPDTPK"
     gene            7121..8119
                     /locus_tag="PKV31_04585"
     CDS             7121..8119
                     /locus_tag="PKV31_04585"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HNB92168.1"
                     /translation="MNLQHIQAAHCESGVCVNLLSHYGISLSEPMIFGSGSGIFFIHI
                     PFLKVNGVPATSYRIWPGAIFNRSMKLLGAKVVTKKFKSPEKSMKALDDLLEQKIPVG
                     IMCSVYHLPYLPDAYRFHFNAHNIVVWAKDGDEYIVGDPVLPDMKRIHKDDLMKARYA
                     KGFPEPSGKLYYVKSLPNGEINYRKLVRRGLKRTFFLINSAPLSFIGVKGIRYLAGKI
                     ARYPEKMGSRKSMLALGNLIRMQEEIGTGGGGFRFMYAAFLKEASHRLDNEELGHLAD
                     EMTAIGDMWRNFAYEVAKVIKNRSSSENAFDEISTLLGKIGDCEVAFFKKLARVKI"
     gene            8116..8895
                     /locus_tag="PKV31_04590"
     CDS             8116..8895
                     /locus_tag="PKV31_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB92169.1"
                     /translation="MNSIPAADHISNNPDARGITIHHVTKRYSGQPAAAVDNINLTFE
                     GGAINGLLGPNGAGKTTLILMICGLMPPDKGQIGRMKNGILQNLDKRKIGFVPQQDGL
                     FGELTLQENLEYFGGLYCLSRKELKTNIERLSSYLQLSNHLGKKIKYFSGGMNRRANI
                     LASLLHDPEDIIFDEPTAGVDVQSRALIHRLIMDLKEAGKTILYTSHLLAEAEELCTE
                     IYIIDHGKLILSGKPKHLLRQHGKAGLDELFLSLTGKQVRD"
BASE COUNT         2771 a         1814 c         1846 g         2545 t
ORIGIN      
        1 attgtaatta cagttgagcg agctgaccca gagctgctac cagggtccat ttttcaaatt
       61 cacttaccct ggcaccaacg gtattagctg tgctaatttt agcgaatccg ggagcggcat
      121 tgttcagatc ctcatcgtga gaacccttat cccggaaaat gtcagccagg gaaggttgtg
      181 tattcatgtg gctgacatta aactccaggt tagcgaaaag tatttttcct gaaaaccatg
      241 agtctgagac tccctggtca tttagcctga attctgaacc ttcagaaaaa ttaaagaaat
      301 ataaattttt caaagaaaca gagaccttgg ctcttaggtc agcatactta cctgaggtgg
      361 cggcagtatg tccctttaat gtgccgtttg agatggtgta tgtaggattt tcaacacctt
      421 cacctccatc cagttcaaga gcgctgtcgg tctggtcacc tgcaattccg atgatgttgt
      481 ccatggtccc ggaccatccc tgatcgcagt caaacatatc atcgcctgta ttccagacca
      541 taatattctt agggttgacc gtacctccga atatttcgat gccatcatcc tgattggaaa
      601 ctatttcgat gttttcgatg atggtgcctg atcctacacc agccagcgtc aggccgttga
      661 tttcattacc ttcgccgatg ttggttccac cgtgacggat tgaaacatat ttcattatcc
      721 ctgaattgtc ctgcgggtta ttgccaccat aaaggccatt aatgtcggaa ggtggaatac
      781 cttcaatctg aacagaaggc gcattggctg atataggagc atctccgagg atgagcactc
      841 cgccccagag gccattgatt ccctgatcca gattagggct tacgatttcg ccggatttta
      901 tttcatcggc aacagaagtg aagatgatgg gcttgtcaac ggtaccctga gcgtcaatct
      961 tcgccccttt ggcaattagg agggcagtag cattggctcc acttcctgcc tgacctttaa
     1021 taacagttcc gggcatgatg gtcaatttga cgccgggtaa aacagttact ctctgtgcca
     1081 ggacatagac cttatcagct gtccaggtca catcagacga aatatttccc gagacgatta
     1141 cagtactatt gtcgcccaca gggacacact cacagttatc ggtcagtact tcattggggg
     1201 cacaggtttt gatacattca tctttatcac atgctgtaaa taaaaccggg atacatgcaa
     1261 gaaatacgaa taacagattt ttaatgtttg tcatataaat tcatttttgg ttagacattt
     1321 tcttcaaata ttccgcaaaa atgaatcggc aacattaagg aaatattaat tctatatgac
     1381 gatattgtta agtcctccaa atggttaata ttaagtcttc agaggaaaag aatcattgaa
     1441 atccggtgaa atatggagtg agaaaacaaa ataaagtgtt tgatggatga atttattatc
     1501 caaaggaatg atgggtggga tgtgaattaa ggatttatgg aaataagtgg tgataaagga
     1561 gacttgttgt atttccagat agccaggcca tacgggaaat gacaagggat tcgacaccat
     1621 ggtaggcatt gtaaaaaggt gtcctccagg ttatctttta cacgaggatt cattaaagtg
     1681 cggttgaaat tcaggtattc attttgaatt aaaacactaa ggtatttccg tattgttttg
     1741 agccgggatg gtggtgcctc atgtcgggat cgtgctgtag caccagtcgc cggaatttga
     1801 atgaatggtc cgtatgtacg gtatgggcca gttcatggat gattacctgg tcgggagtct
     1861 caaggggtgc aaggaggagc cggttggaga gtataatgtt gttttgggat atacaggatc
     1921 cccggtgaga gacatcatta cggagggtgt atatccgtat ctaaaatttt aactcaagtt
     1981 gtcttttaat caacaaattt tattaaaaaa cgataaaaaa ttggcaaatc tcaacttgaa
     2041 aagggacatc gctctgtatg aaaaatcaca aattacagca gttcaatcga tgtttggtta
     2101 ccggatttcc acgggaaatc ggatgttata agaaataaga aatatctgtt cgccaataaa
     2161 tctgtagtac acaaagccga gcaccttctc ccggaaagtc ccaaactgtg cagaatatca
     2221 tttgcctatg cgcaattggg ttaactaatt gccctatcta ttcaaaggca attcaaaacc
     2281 taaataaaat atttatctga taccacgaaa gagcaaaatt cacagggtga aattatagaa
     2341 gcttcaaaca gctatgtatt tatacactcc aaagacctca tttctttttc cctgacttca
     2401 tttcgctctc tttcatgaat ctttgaggga ggaatgaaat atggcgccat ctgcctcttt
     2461 tatatttaaa gccttcatag gttccatccg gcacatacgt atcgccctga ccaacaaaga
     2521 tttctcgcat tggtatcaga tgatccagga caatcatttt ttcttcattg tcataattaa
     2581 gtttcatagc gacgtcagcc gaataggtat aaatatatct aagccgatca tccccataat
     2641 cggttgaatc tctgataaat acatccttac caaataaggg ccgtccatcc tggtcgaaag
     2701 aaagtacttc aatgaccttc tgcttgttga aaaaagaatg agcattgtat ccaagtaata
     2761 aataatattt atcccccttc aatttacaat caaaaagccg ataatacaag gctccttgcc
     2821 attcatcggc cctcagttct tcattagtaa ttccttcgga aaaattctca taggaccgat
     2881 ccgtaaatgg gataaaacct cccgaagcct tttgtattcc accataataa cgatattcgt
     2941 catccttgat aaaatgttgg ccggtcaata tcctgaaaga tgaatctgtc ggataaagca
     3001 ctgaaaaaaa atctgacttg acgaatttct tcttaaaagt tgcagggtct tgaagcaatg
     3061 tattgaataa gtcattgacg tgttctacag cggcctttct ttctgttgaa ttagtatccg
     3121 taaggattat tttacccatt gtactaagtt cttcatcata ttgctcccag gttgtatttt
     3181 gggcatgcaa accgaaaacc attccaaagc atagaatgta taacaggata tatttcacgt
     3241 cagatttaat taattgaggg ttaagttaat caagttttaa gttgtattgc gattggcatt
     3301 aatagaaacc tttgtaaaat tattcaatca tcgtccataa cgtcaattcc caggcaatcc
     3361 atacttataa atttgttaat tcattttatt agggtaaaat taataacaat gctgaacaaa
     3421 gaatttcacc atttgttatt ccatcttctt gatctactgt aatccttgaa gacaaaatca
     3481 cctaatcctt gcaagtcact ataaaatgcc ttgcctccat tcgtttcggt aaaaagttca
     3541 acaaattcca tcaaataggg atctttagca atcatgaagg tagtgatagg tatgccaagc
     3601 cgtctaaact tgccggcttg attgagggta cggttgacaa tcatcctgtc gagaccaaag
     3661 ctatttttgt acagcttatt acctcgtttg atacaagtcg gctttccatc tgtaatcatg
     3721 aagacctgtt tgttggtatt ttttctcttt cggagaatgt ccagtgccaa ttcaagtgca
     3781 gccaatgtat tggtatgaaa cgggcctacc tccagatagg gtaattcatc cttttcgatc
     3841 ttccaggcct catccccgaa cacaacaata tcaagagtat cctttggaaa tctggtcgat
     3901 atatattccg acaaagccat ggctaccttt ttggccggag taatcctgtc ttcaccatac
     3961 aatatcatcg agtgcgaaat atcgataagt aagacgctac tcatactact ctggtgatag
     4021 gtttcacgga tttccagatc ctcatactca aggctgaaag agcccggtcc atggttgatc
     4081 tgtgcattct ttatcgattc gacaaatgcg atctgttcag ggctgtctcc gaatacatat
     4141 ggccgcaagt ctgaagtcaa ttcatcaccc ttgccatcct tttttgtagt atgattgcct
     4201 ttagtggctt tctttatgtt gccaaaaact tccttcaacg ctttacgacg taaggaaata
     4261 ttcatcttct ctgcaggtct caaagcattt tcctcgggat cggctttcag ataacctttt
     4321 tgcatcaatt cgtctaaaaa atcctggata ccgtagtcat ccctcgtaag attatactcc
     4381 cgatccatct ccatcaacca ttgaatggcg gtcgctacat tacctccggc atacatcaat
     4441 agatccttaa acaggttgag cagctcatca aaaagagaca actccggttc accatcgtat
     4501 tttctaaatt gccaaccaaa cattttccaa tatttgaatt attaaattcc ccttgtcaac
     4561 atccacaatc cattgtggtt atattattct actttacctt gttcgtggac atctttacat
     4621 caaacttaaa agaatacaac cttcatgcgc tgtcaaatac atttttaaag aaaataaaat
     4681 ttcatgtaga taaaatcaaa caaatacaac aattataaaa cgcttttcgt gcttgtcaaa
     4741 tagtttaaaa gcttaccaat aaccatactt ttactcttca gataaaatgt ataaccgaaa
     4801 atggattact tttgttgctg ttcgggaaac ttgtaccaaa ttcaattgta aaacaaatta
     4861 taccgtaact ttgttagtgt cggaattgat tttttcaata gtttcaccat caaagttgta
     4921 tactgtcgga aataaattat gtgccaaaaa ataaaatatc tttttgtccc cgtctgggta
     4981 ttaatctcat cctcattact ttttgcgcaa gataaaaatc atgatgaatt tgtaaaaagt
     5041 atttgcggaa cgtattccgg gaattacatc accaaaaaag gagttcctaa aaacggagcc
     5101 gagatggtac ttatgtatca ggaccatcac actatccaag taatcgaatc cgacttcgga
     5161 gcattcaaaa tatatttgga cgatatctct ccggacacct tgtatccaag gtcgagtatg
     5221 gaagggatgc ggattaaata taccaaaagt tcaaaaagga tatttatcaa tgctcccatc
     5281 caaggcataa ctacgaccta tatcggcaag ttcaggtaca gggattcagg tgatcttgaa
     5341 aggcaaaagg cggcatacga ttcaattatg cgccataagt cgaaggaagt caagacctat
     5401 ggtacttact acggtacaat tttcaattcc aagactaaca aaagacaatc agatacgatt
     5461 aaggtgtttg attttaagat agatcagaca gaaggaaatt ttcaagtaaa gtataagatg
     5521 gccagaatca tattttccgg tgccgattct caaccttacg agatccgggt ggaatataat
     5581 cccaataata agaccatcag gcaatccggc aaatcaagta ttaagctctt tatagatctt
     5641 gaaaacaaag taattcgttt cgaagacaag gtaaagaaaa taaaattcga aggcatcaga
     5701 atcgaacatt gaaattcatg gcaggatccg ttccctataa tattgctatt ctttattaca
     5761 ctcagaccgg gcagttgaag atgcttgcgg aaaaggtatc agaaggattt tctggtgatt
     5821 gtcgggttga tttcattgag attatcccgg ttaagcaatt tccctttcca tggaatacaa
     5881 tagaattttt tgatgctatg cctgaatgcg tattggaaga aggctcagaa ataagactat
     5941 ccggttatga agaggggaag cattacgatc ttgtcatttt ggcctatcag ccatggtttc
     6001 tgcatccatc tctccctata accggttttc ttcaaagtaa atgggctgac aaactcttga
     6061 aggatgtcaa ggtcattact cttaccggag caaggaatat gtggttaaat gccttagaaa
     6121 agataaaacg cgacttgcat aagttcggat cggagcatgt aggcaacatt gcatttgtag
     6181 attcatcacc caatctcatc tccacgctga ctgttattcg ttgggcgttc aaaggccgca
     6241 aggaagcgtc cggattgcta ccggaagcgg gtgtacaggt agctgaaatc aacagaggtc
     6301 ctttgtttgg caaattgatt gccagtgcct tacaagaagg caaatggcaa actcttcaac
     6361 aggaattggt caaagccggg gcagtaaaat tgaaacccgg tctgatcctg ctggaagaaa
     6421 cggcaattgg ccagtttaaa atgtgggccc ggaaaatcaa agcaaaaggt gagcgcggat
     6481 cacctgcccg aaggcccttg gtcagattat tcaagaacat actgattgtg gggattttta
     6541 tcctttcgcc cattaaattt gtagtttcga agatacaagc catgctcaac cggaaaaggc
     6601 tcgttgctgc ggcaaaatat tttcaatccg taaattatga agaaaataaa ttttcctgat
     6661 aactagtcaa agctatgaag aaaaacgaag tgcctcaaga cgacgaagct atctaccagc
     6721 aaaaattcgg agacggactg ctgaaatatg ccactgacga acaaaatgaa tatgtcacgg
     6781 tgcaatcggt tggctgggag cctgagatag tagcattaaa acaagcctgg gaattggtgg
     6841 aggaaaaggt ggagatggca agacaagcag tgaagaataa tcaaaaaagt ccgatctatt
     6901 atttcatgga aaagaaattg atggatacgg gaattttggc aggatatatg ggattatggc
     6961 gatggcaggt ggcaaggcat tttaagccgg gtgtatttgc aagaataaaa ccttcaaccc
     7021 tggaaaaata cgccaaagta tttgatattg acctaaaaca attaacggat ttcgacccgg
     7081 atcatcctga cacacccaaa taaagcatga aaaaaaattg atgaacttac aacatataca
     7141 agctgcacat tgcgaatccg gagtatgcgt caacttgctc agtcactatg gtatatcact
     7201 cagtgaaccc atgattttcg gatccgggag tggcatattc tttatccata ttcccttcct
     7261 gaaggtaaat ggagtccctg ccaccagtta taggatctgg ccgggagcaa ttttcaacag
     7321 atcgatgaaa cttcttggag caaaggtggt tacgaaaaaa tttaaatcac cagaaaaatc
     7381 catgaaggcc ttagacgatc tgctggagca gaaaatcccg gtcggcatca tgtgcagtgt
     7441 ctatcaccta ccctatctgc cggatgcata cagatttcac ttcaacgccc acaacattgt
     7501 cgtttgggca aaggatggag acgaatatat agtgggcgat ccggtcctgc ctgatatgaa
     7561 acgtattcat aaggacgatt taatgaaagc tcgctatgct aaaggcttcc ccgaaccttc
     7621 cggcaaattg tactatgtca aaagcctgcc aaacggagag attaactatc gtaaattagt
     7681 ccggcgaggt ttaaagagaa ccttcttcct catcaacagt gcaccattat ccttcatagg
     7741 agtaaaaggt atccgttatc ttgccggtaa aatcgcccga tatccagaga agatgggcag
     7801 tagaaagagt atgttagctc ttggcaatct tatcaggatg caggaagaaa taggcaccgg
     7861 agggggcgga tttagattta tgtatgcagc cttcctcaag gaggcatcac atagattgga
     7921 taacgaagaa ctgggtcatt tggcggatga gatgacggca atcggtgata tgtggagaaa
     7981 tttcgcttat gaggtggcca aagttataaa aaaccgatct tcatcggaaa atgcatttga
     8041 tgaaatctct actttattgg gtaaaatcgg tgattgcgag gttgcattct ttaagaaatt
     8101 ggccagagtc aaaatatgaa ttcaatcccg gcagcagatc acatttccaa taatccggat
     8161 gctcgcggaa taaccatcca tcatgtcacc aaacgatatt caggacagcc tgccgcagcg
     8221 gttgacaaca taaaccttac atttgagggt ggcgccatca acggacttct tgggccaaat
     8281 ggcgcaggaa aaactactct gatactcatg atttgtgggt tgatgccacc tgataaaggg
     8341 caaattggaa ggatgaaaaa cggcattctc caaaacctcg ataaacgcaa gatcggattt
     8401 gtgccacaac aggatggcct tttcggagaa cttacactgc aagaaaatct ggagtatttt
     8461 ggaggattat attgcctgag caggaaagaa ctgaaaacca acattgaaag gctttcttcc
     8521 tatcttcaac tatcaaatca tctcggtaag aagattaaat acttttccgg aggtatgaac
     8581 cgaagagcga atatacttgc atcattgctg catgatccgg aggatatcat atttgacgag
     8641 ccgaccgcag gtgtagatgt gcaaagcaga gctttgatac ataggctcat catggatttg
     8701 aaagaagcag gaaaaaccat cctttacacc agtcatttac tggccgaggc agaagaattg
     8761 tgtacagaaa tatatatcat tgaccacggc aagcttattc tctcaggtaa gccaaaacat
     8821 ttgcttcgtc agcacggaaa ggcagggctt gatgaattgt ttctatcatt aacaggtaag
     8881 caggtaagag attgaatttt caaagaatca aatcgacatt caggaaggag cgaatcctgc
     8941 ttttccggga catgggtggc atagccatgc tgttgg
//
LOCUS       DAOQMC010000079         8940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3055_length_8940_cov_4.451210, whole genome shotgun sequence.
ACCESSION   DAOQMC010000079 DAOQMC010000000
VERSION     DAOQMC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3055_length_8940_cov_4.451210"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1544
                     /locus_tag="PKV31_04595"
     CDS             159..1544
                     /locus_tag="PKV31_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB92170.1"
                     /translation="MDTNSQAEEILSRITDDQDFFKALRSKEYSRLDTPVHQIYLDFT
                     GGNLYPSSLIKKHHDALLNGVFGNPHSINPTSARSSILVESAREKVIEFFNAEEYLCV
                     FTPNATGALKIVGECYPFTSDSHYVLLSDNHNSVNGIREFCKNHGGSVNYVPVQYEDL
                     QINDEILKKALTDNAGQSNSLFSFPAQSNVSGVKHSLDWIKFAQNLGYDVLLDAAAFV
                     PTSRLNLKEVQPEFVSISFYKIFGYPTGLGCLLIKKSVFDKLKKPWFAGGTVTMVSVT
                     SQHSFLAGNHERFEDGTLNYLDIPALQNGLEFIESIGMERINARIKGLTQYLLDNLHA
                     LHHKNGKPLVKVFGPHDMEQRGGNLVMNFFDENGNTIDFESIDVVARDKNISIRSGCF
                     CNPGIDEINNCITNDEMAQYFTSRDKGDYHDMVKYLGKMRGATRVSVGIATSKEDLDA
                     FIEFLKEHFKI"
     gene            complement(1559..2068)
                     /locus_tag="PKV31_04600"
     CDS             complement(1559..2068)
                     /locus_tag="PKV31_04600"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNB92171.1"
                     /translation="MEGTSDVFTVENYLYFTRLIIGVVLCYLLYINFPELPFQWSLVS
                     VVVAISPDNSTQLAKDRMIANLLGCVVGLVMFFIHPPNLLMLSIGVMLVVIIGLYFNM
                     QGSIRSALAALVILMLYNNQSADWTLALDRLVCVIAGCLVALLVTLAFNYLFKVCRFK
                     SPQPKDQAT"
     gene            complement(2126..2767)
                     /locus_tag="PKV31_04605"
     CDS             complement(2126..2767)
                     /locus_tag="PKV31_04605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNB92172.1"
                     /translation="MVELYKSASPIVTVIMVTYNRAEYLDRSIGSFLNQTFKNTELLV
                     IDDGSEDNTFEVVLKYMMAHDNIRYFRHSNRNISLSKNVGLKAAAGRYITFLDSDDAL
                     KPDYLESRVDYMEHHPEMDMIEGGVIIIGNPYVKDRNNPERLIHLSECHIGATYFGKT
                     EVFLSSGGYDKSITYSEDSQFWTKVEKKYKTGKFDYPGYIYYRDTPGSVCNTV"
     gene            complement(3097..4368)
                     /locus_tag="PKV31_04610"
     CDS             complement(3097..4368)
                     /locus_tag="PKV31_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HNB92173.1"
                     /translation="MSKELKRKMKPESLMMSYGYKPELSEGAVKCPIFQTSTFVFKNA
                     EEGKAFFEVAYGHRNPNEGEELGLIYSRINNPDLEILENRLTLWDEAEDCAVFESGMS
                     AITTVLLEFLKPGDLLLISSPLYGGSDHFIRKILPKFGIHVAEFRPGQSKSEILRIVD
                     QSGHASNLALVYIETPANPTNDMVDINLSRQVADHFQEKYGSKVFLAVDNTYMGPLWQ
                     HPLKHGADLVLYSATKYIGGHSDVIAGACLGSFELIKRVKGLRTFLGNMASPNTGWLL
                     LRSLETLKARMDIQSFNANKVAQYLNGHPMIEKVYFPGNLTEADGEQFEIKSRQCMTN
                     GGMISFDIKGGEKEAFKFLNALDLIKLAVSLGSTESLAEHPATMTHVDVDPKLRRELF
                     ITEKMIRLSIGVENYEDIIYDIGQALEQAKS"
     gene            complement(4402..5076)
                     /locus_tag="PKV31_04615"
     CDS             complement(4402..5076)
                     /locus_tag="PKV31_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNB92174.1"
                     /translation="MIPWEEILLRLVLASLFGAAIGLERERKTLAAGLRTHMMVCVGA
                     CLIMLVSSLGFADILGTPNVMLDPSRIASQVVSGIGFIGAGTILFMKQGTVHGLTTAS
                     GLWTVAAIGLATGGGMYFAAGVTTAIALIILWGLQQLEHIYSRRYKNKILKIVIPSEN
                     HTPEVFARLFEDDTLGIQSLSFEKDAKDTIYIVKFEEAEDSRFVPLMNQLKTISEIKS
                     VFWENA"
     gene            5288..6085
                     /gene="proC"
                     /locus_tag="PKV31_04620"
     CDS             5288..6085
                     /gene="proC"
                     /locus_tag="PKV31_04620"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNB92175.1"
                     /translation="MKKTIAIIGCGNLGSAIAKGILEKSSDKYNVIATRNKVELLTPL
                     QALGVEVSNNNIDAVAKSEIILLAVKPYLVATILNEIKNVLNPAKVLVSLATGITIAD
                     MKLILGDTIPVLRAMPNTATGVGESLTCLCSADSNSEKRIGEVKNLFDLIGESIVIRE
                     DLMDGATVLGACGIAYVMRFIRAMVQGGIEIGFDAATASKIANQTVKGAAQLLILDQT
                     HPEQEIDKVTTPRGCTIVGLNEMEHQGFSSSLIKGIITSYKKIENTK"
     gene            complement(6245..8872)
                     /locus_tag="PKV31_04625"
     CDS             complement(6245..8872)
                     /locus_tag="PKV31_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB92176.1"
                     /translation="MSQVEMPQPKKKYKFKELKCYASTEWLIDNQKKYRQVFDRYEVS
                     YIYAELSFINKMYDEEDWSVRIDLKCYQVIDNNQRNEICSLHFDRTIYKYDSIVYLRE
                     GWGAKQEGHFWKQGSYYWEVYINGEKEGTKHFYVIDADRPMSDEDNPFFSLLSVKLYE
                     GPYGDVPEDERTYYTEFSGSESQLIYMEMCIDNFLPKTWQCEVFIRIYTSSRELKGQI
                     VRLVNVKPNDSEIKITAGWGTNVKGNWKRGNYTYEVVFMDRLMAIVPFTVGEFFIEGH
                     PAVFLQDPLIPLEFKEIEHDEENFESIMSKIDRYIGLEDIKRAIRQHAAYINFLNIRK
                     QKGLESDAKLNIHSVFIGNPGTGKTTIAGFIGRLYKKMGLLSKGHVHEVDRVDLVGEY
                     IGQTAPKVKQAIDTARGGVLFIDEAYSLARTNDDSKDFGREVIEILVKEMSSPTCDFA
                     VIVAGYPKEMKHFLNSNPGLNSRFKMYYEFPDYLPQELSEIADYVAGDLKVRFTPKSK
                     AMLDEIILEAFRKKDKTFGNARFVYDLVEKGKINLGIRTMSRANPERLSDEQLSTITE
                     QDILKLKQVIRPNLPEIPIDEILLAEARNELESLIGMDNIKKDILEVVEIVRYKRRHG
                     ENLLHKFFFHTILIGNPGTGKTTVSRILAKLFKALGILERGHLVETDRQGLIAGFVGQ
                     TAIKTTEKIDEAIGGVLFIDEAYAIGGSGFNDYGNEAIQTLLKRMEDDRGKFYVFAAG
                     YPDNMDAFIKSNPGLASRFDKTLKFYDYSPEELIEIARKMLADQHYRLSPKALEYLAQ
                     YINFLWQYRDKYFGNARTIRLITDELIKKHNLRLVRENEGHKERQRTNIITMDDVSFL
                     EMKTEDLNFVRKKIGFGSR"
BASE COUNT         2673 a         1915 c         1613 g         2739 t
ORIGIN      
        1 tgaacattct ttgtagataa aaggttgcaa acgaatcatc gcaataccgg taaattaact
       61 tatggaatac tgaagacaat ggggaattat tctatcgtaa taatgtatat tgatattata
      121 tccaaatatt gctaattttg tggtaattct aaaatactat ggacaccaat tctcaagcag
      181 aagaaatact ttcaagaatt acggacgatc aagatttttt taaagccctg aggagtaaag
      241 aatattccag actggatacg cctgtccatc agatctacct tgactttact gggggcaatt
      301 tatatccttc gtctctgatc aaaaagcatc atgatgcatt attaaatgga gtctttggca
      361 atccacattc catcaatcca acatcagcaa gatcttccat tctggttgaa agcgccagag
      421 aaaaagttat tgaatttttc aatgccgagg aatatttatg tgtttttact ccaaatgcta
      481 ccggtgcact caaaattgta ggtgaatgtt atccatttac ctctgacagt cattacgtac
      541 tcctttctga taatcataat tcagtcaatg gaatcagaga attttgcaaa aaccatggag
      601 gctcagtgaa ttatgttccg gtccaatatg aagatttgca aattaatgat gaaatcctca
      661 aaaaagcttt gaccgataat gccggacaaa gcaatagcct cttctctttt ccggctcaga
      721 gtaatgtaag tggtgtgaaa cactcgcttg actggattaa atttgcccaa aatcttggct
      781 acgatgtact tcttgatgcc gcagcatttg taccaacatc cagattgaat ctgaaggaag
      841 tgcaaccgga atttgtcagt atttcatttt ataagatttt cggatatccg acgggattag
      901 gatgtctctt aattaaaaag tctgttttcg ataagttgaa aaagccctgg ttcgccggag
      961 gcactgtaac tatggtatca gttacttctc agcacagttt tttagccggc aatcacgagc
     1021 gctttgaaga tggcacactg aattacctcg atattcctgc cctccaaaat ggtcttgaat
     1081 tcattgaatc tatcggtatg gagagaatca atgccaggat aaaaggtctg actcaatacc
     1141 tattagataa tttacatgca ctgcaccata aaaatggcaa acccttggtc aaggtctttg
     1201 gaccgcatga tatggagcaa cgcggtggca acctggtaat gaatttcttc gatgaaaatg
     1261 gcaatactat tgattttgaa agcattgatg tggtagcaag ggataaaaat atctccatcc
     1321 gatcaggttg tttttgtaat cctggaattg atgagatcaa caactgtatt accaatgatg
     1381 aaatggctca atactttacc agcagagata aaggtgatta tcatgacatg gttaaatatt
     1441 tgggtaaaat gcgcggtgca actagggttt ctgtaggtat tgccacttcg aaagaggatt
     1501 tagatgcttt cattgaattc cttaaggaac atttcaagat ttgatatcac tgtaaaaatc
     1561 aggttgcctg gtccttcggt tgaggtgatt tgaaccgaca aaccttaaat aggtagttaa
     1621 atgccaaagt taccaataat gcgactagac atccggcaat cacacatacc aaacgatcca
     1681 gggccagtgt ccaatctgct gactgattgt tgtatagcat caaaatcaca agtgcggcta
     1741 aagcactccg aatagagcct tgcatattga aatataggcc tataattacc accaacatca
     1801 ctccgatact taacatcaac aggttcggag gatgaatgaa aaacataacc agccctacca
     1861 cacagcctaa taaattggca atcatcctgt cttttgccaa ttgagtactg ttatccggcg
     1921 aaatagcgac aacaactgaa accaaagacc actgaaaagg caactcagga aaattaatgt
     1981 aaagaaggta acataataca accccaatta taaggcgagt gaaatacaag taattttcaa
     2041 ctgtgaaaac atcggatgta ccttccattc gttatattta tattttattt aatatataaa
     2101 acaatcaaat tgatatatat gcatatcaca cagtattaca tacacttccc ggagtatcgc
     2161 gataatagat ataacccgga taatcaaatt ttccagtctt gtacttcttt tccactttag
     2221 tccagaactg actgtcttca ctataggtaa ttgatttatc ataaccaccc gaagaaagga
     2281 aaacttccgt tttaccaaaa taagtggcgc caatatggca ttctgacaag tgaatcaatc
     2341 gctccggatt gttccggtcc ttgacatatg gattcccgat aataataact ccaccttcta
     2401 tcatatccat ttccggatga tgttccatat aatcgaccct ggactccagg tagtcaggtt
     2461 ttagtgcgtc atcactatca aggaaggtta tatatcgacc ggcagctgct ttcaggccaa
     2521 cattctttga cagtgagata ttgcggttac tatggcggaa atatctaata ttatcatgtg
     2581 ccatcatata cttcaaaaca acttcaaaag tattgtcttc acttccgtcg tcaataacca
     2641 gcaactccgt atttttgaaa gtttgattga gaaaagatcc gatacttcga tcgaggtatt
     2701 ccgccctgtt ataagtcacc ataattacag taacgatagg ggaggcgctt ttgtacaatt
     2761 caaccatttc attcatttta tttcacattc ggaaataata ccgaaggtac attcgttatt
     2821 gaccaatttc attgatctat acgactgtaa catcggcatc ccggaacccc ggattgtttc
     2881 actccctgta cagagcagag ctctagtagc atagctatcc ggacaagtat accaaaaatt
     2941 tatggactat tatataaatc atcatggtat aacaactgca aaccattcaa taaacctact
     3001 aacttcagtg gatattttcg atagtcaatt gtaaaattgt ttttctgatc aggaacctga
     3061 accgtgttgt tattgtatca ccattcttct gttatatcag cttttggctt gttccaaagc
     3121 ttgtccaata tcgtaaatga tatcctcgta attttcaaca ccaatagaaa gtctgatcat
     3181 tttctctgtg ataaacagtt cacgacgcaa cttcggatcc acatccacat gtgtcatggt
     3241 agcaggatgc tcagccaggc tttccgtact acccaagctg accgccaact taattaagtc
     3301 aagggcattg agaaatttga atgcttcctt ttctccgcct ttgatatcga atgaaatcat
     3361 tcccccattg gtcatgcatt gtcttgattt aatttcaaat tgctccccat ccgcttctgt
     3421 taagttgccc ggaaaatata ccttttcaat cattggatga ccattcaggt attgtgcgac
     3481 cttatttgca ttaaaggatt gtatgtccat tcttgctttt agcgtttcaa ggctcctcaa
     3541 taacaaccat cccgtattag ggctcgccat attaccaaga aatgtccgga ggcctttgac
     3601 tctttttatc agctcaaaag agcccaaaca ggctccggct atgacatcac tgtgcccccc
     3661 aatgtacttg gtcgctgaat aaagaaccag atcggcacca tgcttcaagg gatgctgcca
     3721 taacggcccc atataggtgt tgtcaacagc aagaaagact tttgagccgt atttttcttg
     3781 aaaatgatca gctacctgcc tgcttaggtt gatatctacc atatcattgg ttggattggc
     3841 aggagtctca atatacacca atgcaaggtt agaagcatga ccactttggt ctacgatacg
     3901 aagaatttca gacttacttt gcccgggcct gaattctgca acatgaattc caaattttgg
     3961 caatattttc cggataaaat gatcacttcc gccataaaga ggactgctga taagcaataa
     4021 atcacccggc ttgagaaatt caagtaaaac cgtggtgatt gcagacattc cgctttcaaa
     4081 aaccgcgcaa tcttctgctt catcccacag ggtcaatctg ttttccagga tttcgaggtc
     4141 cggattattg attctgctgt atataagacc caattcttct ccctcatttg gatttctgtg
     4201 tccataggca acctcaaaga aggcctttcc ttcttcagca ttcttgaaaa caaaagtaga
     4261 tgtctggaag atagggcatt taacagctcc ttcagaaagt tccggcttat agccataaga
     4321 catcataaga ctctccggct tcatttttcg ttttaattct ttactcatgt attataatgt
     4381 taaatgatat aattgggtat ttcaggcatt ttcccaaaat acactcttga tttcggaaat
     4441 cgtttttaat tgattcatca atgggacaaa ccgactgtct tcagcttctt caaatttgac
     4501 gatataaata gtgtccttag catccttttc aaaggaaagt gattgaattc ccaatgtgtc
     4561 atcttcaaac aagcgggcaa atacttcagg agtatggttt tcagaaggaa tgacgatttt
     4621 tagaatctta tttttatacc tcctggaata aatatgctca agttgctgca acccccacaa
     4681 aataatcaaa gctattgctg tggtaactcc ggctgcgaaa tacatgccac cacccgttgc
     4741 taatccgatg gcagccacag tccaaagacc cgaagcagtc gtaagcccgt gaaccgtacc
     4801 ttgtttcata aacaagatag ttcccgcacc aataaagcca atcccactta ctacctgtga
     4861 agcgatgcgg gaagggtcaa gcattacatt aggtgtacct aaaatatcag cgaagcctaa
     4921 tgaagaaacc agcatgatga gacatgctcc tacacatacc atcatatgcg tccgcaatcc
     4981 tgcagcaagt gttttccgtt ctctctcgag accaatggca gcaccaaaga ggctggccag
     5041 gaccagacgc aatagaattt cctcccaggg gatcataatt tgcaatattt attacaccta
     5101 ttgatttcag aataaatctt tagaaatgtt aatttcctaa tttgtccggc agtgaaggta
     5161 gacatatttt ttgaatcctg caatattgga ttagcaccat cattcttgac tttgtgactg
     5221 gtaaaaaagg gattaaattt tacaagatcg attttttact acctttgcca ttgaaaattt
     5281 cgtataaatg aaaaaaacta ttgcaatcat tggttgcggc aatcttggaa gcgccattgc
     5341 caaaggaata ttggagaaga gctctgacaa gtataatgtt atcgcaacca gaaataaggt
     5401 tgagcttctt actcccttgc aagcacttgg tgtcgaagta tccaataata atatagatgc
     5461 agtggccaaa tcagaaatta ttctactggc ggtcaagccc tatttagttg caaccattct
     5521 aaatgagata aagaacgttt tgaatccagc taaagtattg gtttctctgg ctacaggtat
     5581 taccattgcc gatatgaagc taatcttggg tgatactatt cccgttttaa gagcaatgcc
     5641 caatacggct accggtgtag gagaatccct cacatgccta tgctctgccg atagtaactc
     5701 tgagaaaagg ataggggagg tcaagaactt atttgattta attggtgaat ctattgttat
     5761 cagagaagat ttaatggatg gcgcaactgt tcttggagca tgtggtattg cctatgtcat
     5821 gcgctttatc agagcaatgg ttcagggcgg tattgaaatc ggctttgatg cagcaacagc
     5881 cagtaagata gccaatcaga ccgtcaaagg tgctgcacag ttgctaatat tggaccaaac
     5941 tcatccggaa caggaaatcg acaaagtcac cacaccacgt ggttgtacca ttgtaggatt
     6001 gaatgaaatg gaacaccagg gcttcagttc ttctctcatc aaaggaataa ttacatccta
     6061 caaaaagatt gagaatacca aataaaattg aaaccggtgt ttagttgtgt tcattaacct
     6121 acgcagaaat tcactttcta agtatatcag cacttataat ctataacagg acaagaaaac
     6181 cggtctatac aaataagtat caaatcaaac ctgaatgaag gagatgctgg aatccatctg
     6241 gccgctatct gctgccgaag ccaatttttt tacgaacaaa gttcaaatct tctgttttca
     6301 tttcaaggaa tgaaacatca tccattgtaa tgatgttagt ccgttgacgt tctttatgtc
     6361 cttcattctc cctgaccagt ctcaaattat gctttttgat caactcatcc gtgataagtc
     6421 gtattgttcg ggcattgcca aagtatttgt cacgatattg ccacagaaag tttatgtatt
     6481 gagcaagata ttccaatgcc ttgggagaca gcctgtaatg ttggtctgcc aacatctttc
     6541 ttgcaatttc aatgagttct tcaggagagt agtcataaaa tttcaaggtt ttgtcgaacc
     6601 gactggccaa gccgggattt gatttaatga atgcatccat gttgtcagga taacctgctg
     6661 caaaaacata aaattttcca cgatcatctt ccattctttt gagtaaggtc tggatggctt
     6721 catttccgta atcattgaag cctgacccgc caattgcata tgcctcatcg ataaacaata
     6781 ctcctccaat cgcttcatca atcttttctg tggtcttaat agctgtctga ccgacaaacc
     6841 ccgcaatcag gccttgtcgg tcagtctcca ctaaatggcc tctttctaat attccaagag
     6901 ccttgaacag tttggcaaga attctggaaa cagtcgtttt tcctgtcccg ggattaccaa
     6961 tgagtatggt gtggaagaaa aatttatgaa gaagattttc accgtgtctt cttttgtatc
     7021 ttacaatttc tactacttca aggatatcct tcttgatatt gtccatacca atcagcgact
     7081 caagttcgtt ccttgcctct gctaaaagaa tctcatcaat tggtatttca ggcagattcg
     7141 gccggataac ctgtttaagt tttagaatat cttgttcggt aattgtcgac agttgttcat
     7201 cactaagtct ttcaggattc gcacggctca tagtccttat cccaagatta atttttcctt
     7261 tttctaccag gtcataaaca aaacgcgcat tgccaaatgt cttatccttt tttctgaatg
     7321 cttccaggat tatttcatcc aacatagcct ttgacttggg ggtaaatcta actttcaaat
     7381 caccggcaac ataatcagca atctcactta actcctgcgg caaataatca gggaattcgt
     7441 aatacatttt gaatctggaa ttcaagccgg gatttgaatt gaggaagtgt ttcatttcct
     7501 ttggatatcc ggctactatc acagcaaaat cgcaggtagg gctggacatt tccttcacca
     7561 ggatttctat gacctccctg ccaaaatctt tagagtcatc attggttctc gcgagtgaat
     7621 atgcttcatc tataaagagg acgccccctc tcgccgtatc aatagcctgt ttgaccttag
     7681 gtgctgtctg accgatgtat tctcccacca gatctactct gtccacttca tgcacatgac
     7741 cttttgacaa aaggcccatt tttttgtaca accggccaat aaatccggcg atggtcgttt
     7801 ttcctgtccc cggattacca atgaagacgg aatgaatgtt tagcttggca tccgactcga
     7861 gacctttttg cttccgaata ttcaagaaat tgatataagc agcatgttgc cttattgctc
     7921 ttttaatatc ttccagtcca atataacgat caatttttga catgattgat tcaaagttct
     7981 cctcatcatg ctcgatttct ttaaattcca gtgggattaa aggatcttgc aggaagacag
     8041 ccggatgtcc ttcaatgaaa aattcgccga cagtaaatgg tacaatagcc atcaacctgt
     8101 ccataaagac aacctcgtaa gtgtagtttc cgcgcttcca gtttcctttt acattggtcc
     8161 cccaaccggc ggtaattttt atttcactat cattcggctt gacattgact aaccgcacta
     8221 tttgcccttt caactcccgg ctactggtat atatccttat aaacacctcg cattgccagg
     8281 ttttgggcag aaagttatca atacacatct ccatgtagat caactgtgat tcactacctg
     8341 agaattcagt gtagtacgtt ctctcatctt ccggaacatc accataagga ccttcatata
     8401 gcttaacaga caaaagtgag aagaatggat tatcttcatc actcataggc ctgtcggcat
     8461 caatcacata aaaatgctta gtaccttcct tctccccatt gatatatact tcccaataat
     8521 aacttccttg tttccaaaaa tgaccttcct gcttcgcacc ccaaccttct ctaagataaa
     8581 caattgaatc atacttgtat atcgttctgt caaaatgcaa tgagcaaatt tcatttcttt
     8641 gattattatc aataacctga taacacttca gatcaatcct taccgaccaa tcttcctcat
     8701 catacatctt attgataaat gacagctcag catagatata actgacttca tatctatcaa
     8761 atacctgacg atattttttc tggttatcaa taagccattc cgtagatgca tagcatttca
     8821 attccttgaa tttatacttc tttttaggct ggggcatttc gacctgggac atctttcagt
     8881 ttaatttgtt ggttgtgggt taattgttgt gcaatatatg cattttttaa aattattaat
//
LOCUS       DAOQMC010000080         8931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3061_length_8931_cov_5.465525, whole genome shotgun sequence.
ACCESSION   DAOQMC010000080 DAOQMC010000000
VERSION     DAOQMC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3061_length_8931_cov_5.465525"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1993
                     /gene="gyrB"
                     /locus_tag="PKV31_04630"
     CDS             32..1993
                     /gene="gyrB"
                     /locus_tag="PKV31_04630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNB92177.1"
                     /translation="MSENTEALQNNGNYDASNIQALEGLEAVRKRPGMYIGSTDTKGL
                     HHLVYEVIDNSIDEHLAGYCDRITTIIHPDNSISVTDNGRGIPVGMHEKLQKSALEVV
                     MTVLHAGGKFDKNTYKVSGGLHGVGVSCVNALSTYLKAEVHRNGHIYQQEYSEGKPTS
                     EVEVVGDAEDTGTIITFRPDAKIFETLEYKFETLSNRMRELAYLNKGLTLILIDERPD
                     ENGKTRNETYHSDGGLAEFVTYLDQSRTSIISKPIHVIGKEDHIEVEVALQYNTSYAE
                     NIYSYVNNINTREGGTHVRGFRMAVARLFKQYGEDNGFFKKLKFTVSGEDFREGLTAI
                     VSVKVPEPQFKGQTKGELGNSEVTSVVSQIVGETLRHFLEENPNEAKRILEKVVLAAT
                     AREAARKAREMVQRKNVLTGGGLPGKLADCSNRHPEESEIFLVEGDSAGGTAKQGRDR
                     HFQAILPLRGKILNVEKAMEHKIYENEEIKNMFIALGVSIEEKDGEKILNIEKLRYHK
                     IVIMTDADVDGSHITTLILTFFFRFMRPLIDNGYIYIATPPLYQVKKGQQAIYAWTET
                     DRKEAVAALSRKENDDSVKIQRYKGLGEMNAEQLWETTMDPSRRTLRQVTIEDAIECD
                     RVFSMLMGDEVPPRREFIEANATYAKIDA"
     gene            complement(2082..3629)
                     /locus_tag="PKV31_04635"
     CDS             complement(2082..3629)
                     /locus_tag="PKV31_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNB92178.1"
                     /translation="MNKELIINSLPSEVEIALMENHRLVEIHKQKTSNSYNVGDIYLA
                     KVSKLMPGLNAAFLDIGKRKDAFLHYTDLGPKLPSVIECTQQLVNKTPGFNIHNMDLS
                     KEINKNGKIQEVMDKKSMLLVQILKEPISTKGPRLSCEITIPGRYLVLTPFSNAVTIS
                     KKIINPEERKRLQLLIESIKPKNFGIIARTAAEGRKVADLHDEISNLAQKWQEIEQNL
                     SNAKGPKKVLSEADKTTSLLRDFLSDDYHSVVVNDKEVFNNIKSFMSSIAPEKVKIVE
                     HYNGKLPIFEHFGINKQIKASFGKTATMNSGAYLVIEHTEAMHVIDVNSGPKMSKSEM
                     NNTSLTVNLEAAEEITRQLRLRDLGGLIIIDFIDMKNPDHKKALNNRMVELMAQDRAQ
                     HTILPLSKFGLMQITRERVRPALKINTDEVCPACNGSGKVQPSILLTDEIERDLVYLL
                     TNKSISKVNLSVHPYVHAYLTKGLLNIRMKWYFRHKKWVNIRPNSNFALTEYRFFDIA
                     EDEIRFT"
     gene            complement(3874..4677)
                     /locus_tag="PKV31_04640"
     CDS             complement(3874..4677)
                     /locus_tag="PKV31_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92179.1"
                     /translation="MANNRPTGISRQKKIALGSAILLFCILYFSFSLTDKKAKARKDK
                     ERSLITEATGPEALLKEARLSASGDTLELVERLQHATEGKDTANILDNLKRISGIWYS
                     QRRYGLAGYYAEKVAELEKTATAWSIAGTSYGMGVKVLSEGSEREFCAGRAAKAFEKA
                     ISIEPDATIHRLNLAAIYADAPPKDDVMKGIKMLLDLNKKEPGNTAVINLLGELAIKT
                     GQFDKAIERLEESYRIDPDNPQTPCLLSMAYEGAGQHEKAHELAEKCRN"
     gene            complement(4679..4987)
                     /locus_tag="PKV31_04645"
     CDS             complement(4679..4987)
                     /locus_tag="PKV31_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB92180.1"
                     /translation="MRKADLVATIAEKTGVAKVDVLVSIEHFFTEVKNTLSSGENVYI
                     RGFGSFIIKKRAKKIGRHIKKNEAIEIPEHFIPAFKPAKTFTEQVKNRVTVAPEVEED
                     "
     gene            complement(5174..5944)
                     /locus_tag="PKV31_04650"
     CDS             complement(5174..5944)
                     /locus_tag="PKV31_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNB92181.1"
                     /translation="MLHINLRHLRRKKQMSQQNFADVLGIPRTTYSEYEKGNTEPNLK
                     MLGRMCEALDVTLDQLVKADLTMDNLEVFRNKELRVLAMTVDTEGRNQIELVETRAEA
                     GYMSGMADPEYISDLPRISVPSLQGGRYRAFEISGDSMLPMESGSIIICRFVESLRDI
                     RDDKTYIIITHKDGVVYKRVRKADEEARLIAISDNERFMPYTIAYEDIQEIWQYHAHI
                     GFSDVKHTFRTMMEEKIEDIARKVNELHAASIKKFDKN"
     gene            6422..7621
                     /gene="lysA"
                     /locus_tag="PKV31_04655"
     CDS             6422..7621
                     /gene="lysA"
                     /locus_tag="PKV31_04655"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNB92182.1"
                     /translation="MNNKDLLKIAEQFGTPTYVYDAESIKAQYDKLVGAFSTDTRFFF
                     ACKALSNINILKYIRSLGAGLDCVSINEVMLGLRAGFGPEKILFTPNCVDLAEIEEAI
                     CHKVHINIDNISILEQFGNKYGGSYPIFIRINPHIFAGGNYKISTGHIDSKFGISIHQ
                     LRHIERLVKSTGVNIEGLHMHTGSEIKDADVFIEGLEIMFELAEHFPNLKYLDMGSGF
                     KVAYRDGDMETDVAELGQKVAAAMDRFASDTGKKPELWFEPGKFLVSKSGHFLVRTNV
                     IKHTTATVFAGINSGFNHLIRPMFYDAYHEIQNLSNPSGPERIYTVVGNICETDTFAW
                     DRKLNETREGDILVFRNSGAYGFEMSSNFNSRLKPAEVLFLDGKIHLIRRRDAFEDLL
                     KNQIEVL"
     gene            7780..8466
                     /locus_tag="PKV31_04660"
     CDS             7780..8466
                     /locus_tag="PKV31_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92183.1"
                     /translation="MQQDLHLAGLIISEPTTFLTNLLLAVTGIYLGSRFLSGRHAGPE
                     FIRQYGLFLMLTGISALWGGVFSHGLKVYFAVEYSIPGWITAFVAAYYMAGGAISHLQ
                     YCNLAPFDNDIVVRRIKMVNLLILLSAIIFIFIKPHFIVVAVNAAIALVAAGLFCEWI
                     AYKNTGDKGSFLFLVAVTISILTILLFLFKINAEPWFNTNDLSHLVMIGCMFIFAKGF
                     EQMKINIVSA"
     gene            8463..>8931
                     /locus_tag="PKV31_04665"
     CDS             8463..>8931
                     /locus_tag="PKV31_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92184.1"
                     /translation="MIRVDDSYVENLKVQGLRKPRLNTLYSMSRIFREVYRIPAYIPV
                     SYVCFLEHGVNFQFEHFYNRLLESHGEIIFLDNSHRVANYEARTGKKAYALGPLYPKY
                     RKAHDIRPLGTASGTLAFPSHSSSEFDFSSGYSRYAQDLLQLPERFHPITVCVY"
BASE COUNT         2353 a         2007 c         1970 g         2601 t
ORIGIN      
        1 atctttgtgc tcttttataa agagaatttt tatgtcagaa aatactgaag ccttgcagaa
       61 taatggaaat tacgatgcga gtaatatcca ggcactggaa ggtcttgaag cggtcagaaa
      121 aaggccgggg atgtatatcg gaagtaccga cactaaggga ttacaccatc tggtatatga
      181 agtaatcgac aactcgatcg acgagcatct ggccggatat tgtgacagaa taactaccat
      241 cattcatccg gacaattcca tctctgtaac tgataatgga cggggaatac ccgtgggaat
      301 gcatgaaaag ctccaaaaat cagctctcga ggtggttatg accgttcttc acgccggtgg
      361 taaattcgat aagaatacct acaaagtttc cggcggtctt catggtgtgg gggtgtcgtg
      421 cgtaaatgcc ctttcaacct acctgaaagc tgaggttcac aggaatggcc atatatatca
      481 gcaagaatat tctgaaggga agccaacctc ggaagtcgaa gttgtgggcg atgctgagga
      541 taccggaacc atcataactt tcagaccgga tgctaagata tttgagacgc tggaatataa
      601 gtttgagacg ctgtccaaca ggatgcgcga actggcctac ctcaacaagg ggctcacgct
      661 aattcttatc gatgaaaggc cggatgaaaa cgggaagaca agaaacgaga cgtaccatag
      721 tgatggtggc cttgcagagt tcgtcaccta tctggatcag tcccggactt ccatcatctc
      781 caagcccatc cacgtcattg ggaaggagga tcacatcgag gtcgaagtgg cactacagta
      841 caacacttct tatgctgaaa acatatattc ctacgtcaac aacatcaaca ccagagaggg
      901 ggggacacat gtaagaggat ttcgcatggc cgtagccaga ttgtttaagc aatatgggga
      961 ggacaacggt ttcttcaaga agttgaaatt tacggtttcc ggtgaggact tcagggaagg
     1021 tttgaccgcc atcgtgtcag tcaaggtgcc cgagccgcag tttaaggggc agaccaaagg
     1081 ggagttgggc aactctgaag taaccagtgt cgtcagtcag atagtcgggg agaccttaag
     1141 acacttcctg gaagagaatc ccaatgaggc caagcgaata ctggagaaag tcgtacttgc
     1201 cgccacagcg cgcgaagctg ccagaaaggc gagagagatg gtacaacgaa agaatgtgct
     1261 gaccggaggg ggactgcccg gcaagctggc ggactgcagc aacagacacc ctgaggaaag
     1321 tgaaatcttc cttgtcgagg gggactccgc gggtggtacg gcgaagcagg gtagagaccg
     1381 tcattttcag gccatcctgc ccttgagagg taaaatcctc aatgtggaaa aagcaatgga
     1441 gcataagatc tacgaaaatg aggagatcaa gaacatgttt atcgctctcg gtgtaagtat
     1501 cgaggaaaag gacggtgaga aaatcctaaa catcgaaaag ctccgatatc ataagatagt
     1561 catcatgacg gatgccgatg tcgatgggtc tcatattacg acattgatac tgacattttt
     1621 cttccgattt atgagaccat tgattgataa tggctatatc tatattgcaa cgccaccgct
     1681 ttatcaggtc aagaaaggcc agcaagcgat atatgcctgg acagagacag acaggaaaga
     1741 agcagtagca gcactttcca ggaaagaaaa cgatgacagt gtgaagatac agcgctataa
     1801 aggtcttgga gagatgaatg ccgagcagtt gtgggagacg acgatggacc catccagaag
     1861 gactctccgt caggtgacaa ttgaagacgc aatcgaatgc gaccgcgtct tctccatgct
     1921 gatgggtgat gaagttcctc ctcgcaggga atttatagag gctaatgcca catatgccaa
     1981 gatagacgcc tgatctccgg ataaatttgt ttcatttata tataaaaaac gccccatctc
     2041 ccgatgaggc gttttttctt ctctgaagtt gaaagtaatt ctcaggtaaa tctgatttca
     2101 tcttcggcta tatcgaaaaa acgatattcc gtaagggcaa aatttgagtt tggtctgatg
     2161 tttacccatt tcttatgcct gaaataccat ttcatccgga tattcagcaa gccttttgtc
     2221 aggtaggcgt gtacatatgg gtgcacactc aggttgacct ttgaaattga tttgttggtc
     2281 aaaagataga cgaggtctct ttcgatctca tcagtaagca ggatggacgg ctggaccttt
     2341 ccgcttccat tacaagccgg gcaaacctca tcggtattga tcttcaatgc cggtctaacc
     2401 ctttctctgg tgatctgcat caaaccaaat ttactcaaag gcaaaatggt gtgttgtgcc
     2461 cgatcctgag ccatcagttc aaccattcgg ttgttgaggg ctttcttatg atccgggttc
     2521 ttcatatcga tgaagtcgat gatgataagg cctcccaggt ctctgagtcg caactgcctg
     2581 gttatttctt cggcagcttc aaggttgacc gtcagtgatg tgttattcat ctctgacttt
     2641 gacattttgg gtccgctgtt gacatcgatg acatgcatag cttctgtgtg ttctatcacc
     2701 agatatgcac cactgttcat cgtagctgtt ttgccgaagg atgccttgat ctgcttattg
     2761 attccaaaat gttcaaaaat cgggagctta ccattgtaat gttctacgat cttgaccttt
     2821 tcgggagcaa tgctcgacat aaacgacttg atgttgttga agacctcctt atcattgacc
     2881 accacactgt gataatcatc agacagaaaa tcccggagca ggcttgttgt cttgtccgct
     2941 tcgctcaaaa ctttttttgg gcctttggca ttggatagat tttgttctat ctcctgccat
     3001 ttctgggcaa gattgctgat ttcatcgtgc aggtcagcga cttttctacc ttctgctgca
     3061 gtcctggcga tgataccgaa atttttgggc ttgatgcttt ctatcaataa ttgtagcctt
     3121 tttctctcct ccggattgat aatcttttta gagatggtca ctgcattgga aaatggtgta
     3181 aggaccagat acctgccggg aattgttatc tcacaactga gacggggtcc ctttgtcgaa
     3241 ataggttctt tcaggatctg aaccagcaac atggatttct tatccataac ttcctggatc
     3301 tttccgtttt tgttgatctc ctttgaaaga tccatattgt ggatattgaa tcccggagtc
     3361 ttgttcacca gctgctgtgt acattcaatt acagaaggca gctttggtcc cagatcggta
     3421 tagtgcagga aggcatcctt cctcttgccg atatccaaaa atgcagcatt gagtccgggc
     3481 atcaatttac tgacctttgc cagataaatg tccccgacat tgtaagaatt actggttttt
     3541 tgcttgtgaa tctccaccaa tcggtggttt tccatcaagg caatttcaac ttcagaaggt
     3601 aaagaattaa taatcagctc tttattcaca tctgaaagtt ttagtaaaat ttctttttca
     3661 aagtatttac cggcatgccg attttattca acatcccggt aaatactgcg gttgaggtac
     3721 ctaagatacc tttatcgatt cttcttctta tgtcgatttt tccttagtct cttttttctc
     3781 ttgtgcgtag cgatcttgtg ccttttacgc tttttaccac atggcataat cgtagatttt
     3841 tttgttaact aatatattac ttgtgcatgt tcttcaattg cgacattttt ccgcaagttc
     3901 atgcgctttt tcatgttgcc ctgccccttc ataagccatg ctcaacaaac acggcgtttg
     3961 aggattatcg ggatcaatcc tgtaagactc ctccagtctc tcgattgcct tatcaaattg
     4021 gccggtcttt atcgccagct caccaagaag attaattacc gcagtgttcc cgggctcttt
     4081 tttattgaga tccagcaaca ttttgatgcc tttcatcaca tcatcctttg gaggagcatc
     4141 ggcatatatg gctgccagat taagccggtg gattgtggca tcaggctcaa tggaaatcgc
     4201 cttttcaaag gcctttgctg cgcgtcctgc acaaaactcc cgttcactcc cttcagaaag
     4261 caccttgacg cccataccgt aacttgtccc cgctattgac caggcggttg ccgttttttc
     4321 caactcagca actttttctg cgtaataacc agccaggcca tacctccttt ggctatacca
     4381 gatcccggaa attctcttca gattgtccag gatatttgcc gtatccttgc cttcagtagc
     4441 atgctgcaat cgttccacca gttctaacgt gtccccggat gctgataacc gagcttcctt
     4501 caacagcgct tccgggccgg ttgcttctgt gatcagagag cgttctttgt cttttctcgc
     4561 ctttgccttc ttgtctgtca ggctgaagct aaaataaagg atacaaaaaa gtaaaatggc
     4621 ggaacccagg gcgattttct tttggcggct gattcccgtc ggcctattat ttgccatatt
     4681 aatcctcttc tacttccgga gcgacagtca ccctgttttt cacctgctct gtaaaagtct
     4741 tagccggttt gaaagcagga atgaaatgct ctggaatttc tattgcctca ttctttttga
     4801 tatgacggcc tatttttttt gctcttttct tgatgataaa tgatccaaaa ccgcggatat
     4861 atacattttc tccgctggac aatgtgtttt ttacttccgt aaagaagtgt tctattgaca
     4921 ccagtacatc cactttagcg actccggtct tctcggcaat cgtggcaact aagtcagctt
     4981 ttctcattat ttgttggttt ataataagtt atgaaataaa aattcaggct cttataaagc
     5041 gtggcaaatt tcgtattttt ttcattttaa aaaaatttct gactactttt tttcataatt
     5101 tgttgaaatt cacagagtag gataaaatca catacgtcgc acaggccgcg caatacaggc
     5161 aattaatgga cctttaattt ttgtcaaact tttttataga agcggcatgt agctcgttga
     5221 ctttcctggc aatatcctcg attttttcct ccatcatggt cctgaatgtg tgctttacat
     5281 cagaaaatcc aatgtgtgca tgatattgcc atatctcctg aatgtcttca tatgcaatgg
     5341 tatagggcat aaaacgctca ttgtccgata ttgctatcag ccgggcctcc tcgtccgcct
     5401 tcctgactct tttatatact acgccatcct tgtgggtgat gatgatatag gttttgtcgt
     5461 ccctgatatc cctgaggctt tctacaaaac gacatatgat tatagagccc gactccatcg
     5521 gtaacatgct gtctcctgaa atttcaaatg cacgataacg accgccctgc aacgatggaa
     5581 ccgatatcct cggcaaatcc gagatatatt ccggatctgc catgccagac atataaccgg
     5641 cctctgccct ggtctccacc aactctatct gatttctccc ctctgtatcc accgtcatag
     5701 ccaatacgcg cagctctttg ttcctgaata cttcaagatt atccatcgtc agatcggcct
     5761 tgaccagttg atccaaagtc acatccaacg cttcacacat cctgccgagc attttcagat
     5821 taggctctgt atttcccttc tcgtattcgg aataggttgt ccgcggtatg ccgagcacat
     5881 cggcaaaatt ttgctgggac atctgtttct tccgtcgaag gtgtctcaag ttgatatgca
     5941 acattctttg gggcttttgt cgccaaaggt atgtattttt ttacacaatg acaaatatta
     6001 cggcattgtt gacgaaaaat gcggcatctg cttttaaata attcttctat tcaaaaaggg
     6061 cgacaatatc attgtcaaat atccggaaat ttttgcccgt ccggcctcct cctctttatc
     6121 catcaatagc tatagacctt tccaccacag gcttcaacat atgcctctcc atataatgag
     6181 aatgtcatat ccgcctgtct tgccaaagat ctgtcctgct acaatattga ttgctctccg
     6241 ataaaatcct tcttatttaa tgttgaattg cattaaatgg tctaaatatc cctttctttt
     6301 tcataaatga attatatccc tctttggggt gtccgaaacg gctattgtga tttaatcata
     6361 ttattatcct tgtgtttgcg ttacctttgg gcaaatttta ccatcaatct gtaacaggga
     6421 aatgaataac aaggatttac tgaagatagc tgaacaattt gggacaccta cctatgtgta
     6481 tgatgccgaa tccatcaagg cacagtatga taagctggtg ggggcgttca gcacagatac
     6541 acgatttttc tttgcctgca aagcattgtc caatatcaat atccttaaat atatccgaag
     6601 cctgggagcc ggcctcgatt gtgtttcgat caacgaagtt atgctggggc tgagggctgg
     6661 ctttgggccg gagaagatac tgtttactcc caattgtgtc gacctggctg agatcgaaga
     6721 ggcgatttgc cacaaggtac atatcaatat agacaacatc tccatcctgg aacaattcgg
     6781 aaataagtat ggcggctctt atccgatatt tatccgtatc aatccgcata tttttgcggg
     6841 aggcaactat aagatttcaa ccggacatat tgacagcaaa ttcggtatct ctatccacca
     6901 gctgagacat attgaacgac ttgtcaaatc gacaggagtc aatatcgaag gcctccatat
     6961 gcacacggga agcgagatca aggacgccga tgtcttcata gaagggctgg aaatcatgtt
     7021 tgaactggcg gaacattttc ctaatctcaa atacctcgat atgggaagtg gctttaaggt
     7081 ggcataccgg gatggtgata tggaaaccga tgtggctgag ctgggccaaa aagtagctgc
     7141 ggcaatggat cggtttgcct cggatacggg aaagaagccc gaactatggt ttgaacccgg
     7201 caagttcctg gtgagtaaaa gcggccactt cctggtcagg accaatgtca tcaaacacac
     7261 cacggcaact gtattcgccg ggatcaattc aggcttcaac caccttatac ggccgatgtt
     7321 ctacgacgcc taccacgaaa tccagaacct gtccaatcca agtggtccgg aaagaatcta
     7381 taccgtagta ggaaatatct gtgagacgga tacctttgcc tgggaccgta aactgaacga
     7441 aaccagagaa ggtgatatcc ttgtattccg aaattctggt gcttacggct tcgagatgag
     7501 ctccaacttc aactcgagat tgaagccggc agaagtgctg tttttggacg gcaagattca
     7561 tctgatccga agaagagatg cgtttgaaga tctgctgaag aatcagattg aagtgcttta
     7621 gatactgatc aaatactgtt tggttaaatt tcgatctata taagaattga gacggatggc
     7681 aagacaacac aatcacctga ggggcaaaac cgacaatcgg aatacgtaac caaagagaaa
     7741 gagatctgtt tttcaaaacg tattaactat ctttggccga tgcagcaaga tttacacctc
     7801 gcaggactga ttataagcga accgacaaca tttctgacaa atctgttgct tgctgttacc
     7861 ggaatttatt taggatctcg ttttttatcc ggacggcatg ccgggccgga attcatccgg
     7921 caatacggat tattcttgat gcttaccgga atatctgcct tatggggcgg ggtcttcagc
     7981 catggactca aggtctattt cgcggttgaa tattcaattc cgggctggat tactgctttt
     8041 gtagctgctt attatatggc cggaggggca atttcccacc tccaatattg caacctggcg
     8101 ccttttgata atgatatcgt cgtccgacgt attaaaatgg taaacttgct aatcttgtta
     8161 tcagcaataa tattcatttt cataaagcct cattttatag tagtggctgt caatgcagcc
     8221 atagcattgg ttgcggcagg cttgttttgc gaatggattg cctacaaaaa caccggcgac
     8281 aaaggcagct ttttgttttt ggtggcggtg actatcagta ttttgaccat tttgttattc
     8341 ctattcaaga tcaacgctga gccttggttc aacaccaatg acctgtcaca ccttgtcatg
     8401 attggatgta tgtttatttt tgcaaaaggg tttgaacaga tgaaaataaa tatagtcagc
     8461 gcatgatccg ggtcgatgat agttatgtcg aaaacctaaa agtacagggc ctcagaaagc
     8521 cccgattaaa taccctttac agtatgtcgc gcatcttcag ggaggtttac cggattccgg
     8581 cttatattcc cgtgtcatac gtctgttttc tggagcatgg tgttaatttt cagtttgaac
     8641 atttctacaa cagacttttg gagagtcatg gggaaatcat ctttctggac aattcacacc
     8701 gggtggccaa ttatgaggcc cggaccggca agaaggccta tgcgctgggg ccactttacc
     8761 ctaaatacag aaaggcacac gatatccggc cactcgggac agcttcggga accctggcct
     8821 tcccttctca ttcgtcgagt gagtttgact tcagctccgg atatagtcgt tatgcacagg
     8881 acctgctgca acttccggaa cgatttcatc ccatcaccgt gtgtgtgtat t
//
LOCUS       DAOQMC010000081         8912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3075_length_8912_cov_4.696398, whole genome shotgun sequence.
ACCESSION   DAOQMC010000081 DAOQMC010000000
VERSION     DAOQMC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3075_length_8912_cov_4.696398"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="PKV31_04670"
     CDS             <1..1611
                     /locus_tag="PKV31_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92185.1"
                     /translation="YGNNAAGGVINIITTDSQSKKWSLGGSSQIESIGTQNHQLDLGL
                     QTKKWYVDMHGRFFNYDKYPIDSLRLLEKYTLPDGSISTRSKYPWNPKLQMSAGTTVK
                     YKVSADNSLVFKYDLSKESLLDYGPVKRPAFKPYAFDDRFLTSREDISFMYDGLLFHK
                     LYVELKSSYNRFGRHVDNLLFQFQENQYDETQTKSDTTRFRSFFNKLNLSMRITPQLE
                     VLGGVNHTFDAGKGGRIKVENPNEHEAIMSEWAYFSEVRYSLFKRFTFSYSGRFLTHS
                     EFGNNYTSGIKAKADLGKNIVLRASYAQGYRTPDLKERYIEFIDVNHNIIGNPNLQPE
                     KSNDIQLTIQYNPYQWMNAKLNAFRTEINDKISLFQYAPLKYNYSNVDIYTVSGLKWD
                     MSGSWKQLNWALGASLGFWNTIIESEEKPYKGKVLDYNATLNYTIPKVKIAAMLNYRY
                     NGPQPLFSVDFGDVNISEISAASFLDFSLNRYFWKERLQLVLGCKNILNTTYASVSGA
                     DNSGSHRVSGFQFINPGRNYFVKLGINL"
     gene            complement(1732..2304)
                     /locus_tag="PKV31_04675"
     CDS             complement(1732..2304)
                     /locus_tag="PKV31_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNB92186.1"
                     /translation="MNTTEVFYPKSLADWRGWLTENHLSKQSVWLVFYSRTSDKKSIT
                     WSGAVDVALCFGWIDSKKIKIDHETSHQFFSKRKEKSTWSRINKEKVQQLIDQGLMTV
                     AGYESIEIAKQNGSWTILDEVEKLIIPADLNEAFGKHKGSKAYFQGLSKSSQKMMLSW
                     IVLAKRPETRLKRIEEIAILAGQKQKPKQF"
     gene            complement(2307..2459)
                     /locus_tag="PKV31_04680"
     CDS             complement(2307..2459)
                     /locus_tag="PKV31_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92187.1"
                     /translation="MDIASGNHWESMDTDVPCVRKHGIKLATTLIDLKELFSTWTVGV
                     EPTEAK"
     gene            complement(2689..3267)
                     /locus_tag="PKV31_04685"
     CDS             complement(2689..3267)
                     /locus_tag="PKV31_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92188.1"
                     /translation="MINRLTELIWLRWPAVIVSLLLLSCKAEVKQSGAASNPFFQVSD
                     YILDDYRRERSEKALFKTITINGQTDTLLLTDSLNAGETIIMDKLNLNNPDWIPKYKA
                     DTVFGVYGDADRYQYRALDEDLPVQSIEVNMEKGTVSNIRVHNRRRSLINRHDQFLTY
                     IPHTGYRLLSLQRMWNGDTLRMEVSAEFRMMR"
     gene            complement(3264..4793)
                     /gene="gpmI"
                     /locus_tag="PKV31_04690"
     CDS             complement(3264..4793)
                     /gene="gpmI"
                     /locus_tag="PKV31_04690"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNB92189.1"
                     /translation="MEGKKTILTILDGWGIGPVHSSDAIYNAQTPNFDRLWTIYPHST
                     LITFGEDVGLPEGQMGNSEVGHLNIGAGRVVYQALARINKEIREDAVRYNPAVIDMVD
                     YCVRQNKPLHIFCLASDGGVHAHINHLNAFVHLLSEMGVQEGYVHAFTDGRDTGPTTG
                     EGFIRDILEFSKDTSFRLASIIGRYYAMDRDHRWERVKKAYDLLVHGIGDHNPDPLAF
                     VRSSYIANITDEFIEPWMAEGIRHGEAPTVREDDAVLFLNFRTDRPRQLTEVLTQHAQ
                     ESYGLMPMKLYFTTMTQYDEEYKDLHIIYQKDNLTQTLGEIVSEAGLTQLRTAETEKY
                     PHVTFFFSGGREEPFSGEFRTLIPSPKVATYDLQPEMSAQGICQAVLDAMDDKKPDFI
                     CVNFANTDMVGHTGVFEAAVKAAETVDGCLGRITEKALALDYRMIIIADHGNSDYMIN
                     EDGSPNTAHTKNPVPCIFVGNDTQGLTLGEGRLADVAPTILFSMGIKQPEIMTGQNLV
                     Q"
     gene            5154..5531
                     /locus_tag="PKV31_04695"
     CDS             5154..5531
                     /locus_tag="PKV31_04695"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNB92190.1"
                     /translation="MVRTLALFYFLLLTTLVFGQQRITTAIRNGDAKALSGFFNKQVE
                     LTFDDRGDLMTRAEAERMLESFFKTNKVISYTPSHSGKAPDHSGSYTLGNIRTENRHF
                     RIFIKFRPGLGLDSIREVRINSL"
     gene            6063..6929
                     /locus_tag="PKV31_04700"
     CDS             6063..6929
                     /locus_tag="PKV31_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB92191.1"
                     /translation="MKGEYYIKPKNGFLFLLILFLLLGATILLPVMFGDSAISLFAIV
                     PGILFLLVLPGFKVIYPNEAIAFTFFGKYAGTAKENGFFWVNPFYLSKKLTLRARNLN
                     GDRLKVNDGVGNPIEIAAVIVWQVADTFKAAFEVDNYVNYVMVQSEAAVRALANKYPY
                     DQFEDENAQLTLLSGAEKVNDELERALHERLDQAGIRIIEARISHLAYAPEIAGAMLQ
                     RQQAIAIVAARQKIVEGAVGMVDLALTKIKEGSIIDLDDHTKAAMVSNLMVVLCSDRA
                     AQPIINTGTLNH"
     gene            7035..7238
                     /locus_tag="PKV31_04705"
     CDS             7035..7238
                     /locus_tag="PKV31_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92192.1"
                     /translation="MSKKPFILRIDKHTQKLLEKWAADEFRSLNGQIEYLLHRALVLS
                     HRWKEEEIPPEKEEPTNDNSNCD"
     gene            complement(7572..>8912)
                     /locus_tag="PKV31_04710"
     CDS             complement(7572..>8912)
                     /locus_tag="PKV31_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92193.1"
                     /translation="RGFQFRFVNYNYRLGVYSTWHLDYVKLIANQLPGLNQQDIAFTD
                     PPNRILRTYSAIPYRQLSGFEDKELADTTKIRLYNHFLEVAEDLGDPRLEIQEITTGT
                     EIYNGQLVNTVPQLNPPAGFNAFDNPFDPQSIAASVASVPAGQLPLIFRTKYHFDQDQ
                     EVSFLEENNTVTSEAEVGYVLAYDDGSAELNIAAEANNSVKSQIAVKFRLNTGDTLRA
                     VQFHFPRLYDDVSHQLFNLKIWVGSLKEEPDYYYQLQRPVYADTYYDTLQGFTTYPLV
                     NDLTQEPLPLYIPPGDFYIGWQQFSVSSTGQFIPVGFDRNYQGGEALTYYKSTGDWKP
                     MTELTASPLLKGIPMIRAKFQDAWKTSSTTQSKATGFYVFPNPVSSVLNIGASEEMSD
                     NATYHIIDITGKVVMAGDVIKPIDTGGLSEGIYFLLVRNKEGKNLGNARFVKVR"
BASE COUNT         2464 a         1970 c         1911 g         2567 t
ORIGIN      
        1 tatggaaaca atgctgccgg aggtgtcatc aacataatca ctactgacag tcagtccaaa
       61 aagtggagtt tgggcggaag cagccaaatt gaatccattg ggacgcagaa tcatcaattg
      121 gatcttgggt tgcagacaaa gaaatggtat gtggatatgc acggaaggtt tttcaactat
      181 gacaaatacc ccatagacag tcttcgtctc ttggaaaaat atacccttcc ggatggctcg
      241 ataagtacac gctccaaata tccctggaat cccaaactcc aaatgagtgc cggaactact
      301 gtcaagtata aggtcagtgc agataattca ctggtattta aatatgattt gagcaaggag
      361 tctctgctgg actatggacc agtcaaaaga cctgccttca aaccatatgc gtttgacgat
      421 cgttttttga ccagtcggga agatatctcc tttatgtacg atggactcct gtttcataag
      481 ttgtatgttg aattgaaatc atcctataac aggtttggcc gccatgtgga caatctgttg
      541 tttcagtttc aggaaaatca atatgacgag acacaaacca aatctgacac gacaagattc
      601 agatcttttt tcaacaagct aaacctctcc atgaggataa ccccgcaact ggaagtatta
      661 ggcggtgtga atcatacttt tgatgccgga aaaggagggc ggatcaaggt cgaaaaccct
      721 aatgaacatg aagcaattat gtcagaatgg gcctattttt cagaagtccg gtattcattg
      781 tttaaacgct tcactttttc ttattcgggc aggttcctga cccatagtga atttggaaac
      841 aattatactt ctggcatcaa ggccaaggca gatctgggaa aaaatatcgt cctcagggca
      901 tcttatgctc agggatatcg gactccggac ctaaaagaac gttatataga gtttattgat
      961 gtcaaccata atatcatagg caatccaaac cttcaaccgg aaaaatcaaa cgatatccag
     1021 ctgaccattc aatataatcc ttatcagtgg atgaatgcca aactaaatgc atttcgaact
     1081 gaaatcaatg ataaaatttc actttttcaa tatgctcccc taaaatataa ttacagcaac
     1141 gttgatatct atacggtttc cggtttgaag tgggatatga gcggctcatg gaagcagctg
     1201 aactgggcct taggagcaag tctcggattc tggaacacca ttattgagtc ggaggaaaag
     1261 ccgtataaag gaaaggtact ggactacaat gctactttaa actataccat acccaaagta
     1321 aaaattgccg ccatgctcaa ttacaggtac aatggtcctc aaccattatt cagtgtcgat
     1381 tttggtgatg tcaatatatc agaaataagt gctgcgtcat ttttggattt ttctctcaac
     1441 cgttattttt ggaaagaaag gttgcaatta gtccttgggt gtaaaaacat cctgaataca
     1501 acttatgcct cagtatccgg cgctgacaac tcgggtagtc acagagtgtc cggcttccag
     1561 tttatcaatc cggggcgtaa ctattttgta aagcttggca tcaacttgtg aaaccgcaat
     1621 aagcatcttc ttactaacca cccggataaa gatttggcga aagaaactgc ttagcgtgta
     1681 tccgcagtta caaatttgtt ttcacactaa tagtacttgc gtgatttgtt ttcaaaactg
     1741 tttgggtttt tgtttttgtc cggcaagaat agctatttct tcaattcgtt taagtcgggt
     1801 ttcgggtcgt ttcgccaata caatccaact taacatcatc ttttgggacg atttgcttaa
     1861 accctgaaag tacgcctttg aacctttgtg tttcccaaat gcttcgttca aatcggccgg
     1921 aatgatgagt ttttcaactt catccagaat tgtccacgaa ccgttttgct ttgctatttc
     1981 aatactttca taccccgcga ctgtcattaa tccctggtca ataagctgtt gtactttctc
     2041 tttatttatt cttgaccagg tgcttttctc ttttctttta ctaaaaaatt gatgtgatgt
     2101 ctcgtgatct attttaattt ttttgctgtc aatccacccg aagcagagtg caacatcaac
     2161 agctccgctc caggtaattg attttttatc tgatgtccgg gaataaaaaa ctaaccatac
     2221 agattgttta gacagatggt tctcggtcaa ccatcccctc caatctgcaa ggcttttagg
     2281 gtaaaatacc tcggttgtat tcatgattat ttagcttctg ttggctccac cccgaccgtc
     2341 catgtcgaaa aaagttcttt gaggtcaatc aatgttgtgg ctagtttgat accatgcttt
     2401 cgtacacatg gcacatccgt gtccatagat tcccaatggt tgccggaggc tatatccatt
     2461 ttaccttatc tttttattat ctgcaaatgt attcaggatt cttctctggt tgtccatatg
     2521 tttgagattt taacaacatt cccaaatgta aaaggccggt attgggtaga tctctccata
     2581 cttgatttgt tcttcaatct tcagatacgg accaaacata ccagaaagat tttatgtgca
     2641 ccaatccggc gaataaatat ctgggggata atgaataaat accgaatttc atctcatcat
     2701 ccgaaactcc gcactgactt ccattcgcag tgtatcgcca ttccacattc gttgtaagga
     2761 caataacctg tatcctgtat gtggaatata tgtgaggaac tggtcgtggc ggttgatcaa
     2821 actacgtcgg cgattgtgca ctcggatgtt ggacaccgtc cctttttcca tgttgacttc
     2881 gatgctttgc accggcaaat cttcatcaag cgcccggtat tggtatctgt cagcatcgcc
     2941 ataaacgccg aaaacggtat ctgctttata tttgggtatc cagtccggat tgttcaggtt
     3001 gagtttgtcc ataattatgg tttcaccggc atttaagctg tcggtaagca agagcgtatc
     3061 tgtctgcccg ttgatggtaa tggtcttgaa taaagccttt tcgcttcgtt cccttctgta
     3121 atcatccaag atataatcag acacctgaaa gaacgggttg gatgccgcac ctgactgttt
     3181 tacttccgcc ttgcagctta gaaggagcaa cgatacaatc acggcaggcc atcgcaacca
     3241 gatcagttcg gttagccggt taatcattgt accagatttt ggccggtcat aatctccggt
     3301 tgcttgattc ccatagaaaa caggatggta ggagctacat cggccagtct cccttcacct
     3361 agtgtaaggc cctgtgtatc atttccgaca aagatgcatg ggaccggatt cttagtatgg
     3421 gctgtattgg gacttccatc ctcattgatc atgtaatcac tgttaccgtg atcggctatg
     3481 atgatcatcc tgtaatccag tgcaagggct ttttctgtga ttcttcccag acatccgtcc
     3541 accgtttccg cagccttgac cgcggcctca aagacaccgg tgtgccccac catatcggta
     3601 ttggcaaagt tgacacagat gaagtccggc tttttatcat ccatggcgtc caggacagct
     3661 tgacatatac cctgtgcgct catctccggc tgcaggtcgt aggtggcaac cttgggagat
     3721 ggaatcaatg tgcggaattc tccggaaaac ggctcttccc ttccgcctga aaagaaaaag
     3781 gtgacgtgtg ggtatttttc agtctccgcc gtgcgaagtt gtgtcagtcc ggcttcagaa
     3841 acaatttcgc cgagggtttg ggtcagatta tccttctggt agatgatgtg gaggtctttg
     3901 tattcctcgt catactgcgt catggttgtg aaatacaact tcatcggcat cagcccataa
     3961 ctttcctgag catgttgggt cagcacctct gtaagctgtc gtggccgatc ggttctgaag
     4021 ttgagaaaaa gcaccgcatc gtcttcacgg acagtcggcg cctctccgtg tcgtattcct
     4081 tcagccatcc acggctcgat gaactcgtcg gttatattgg caatgtaact actccgaaca
     4141 aaggccagcg ggtccggatt gtggtcgcca attccgtgga caagcaggtc ataggctttt
     4201 tttacacgct cccaacgatg atcccgatcc atcgcataat atcgaccaat gatgctggca
     4261 agccggaagg aagtgtcctt tgaaaactcc aggatgtcgc ggataaagcc ctcccccgta
     4321 gtcggcccgg tatctcttcc gtcggtaaat gcgtgaacat atccttcctg tacacccatc
     4381 tcagagagca agtgaacaaa ggcgttgagg tgattgatgt gtgcgtggac accgccatcc
     4441 gaagcaagac aaaagatgtg caatggcttg ttttgtctga cacaataatc gaccatatca
     4501 atgacggcag gattataccg aactgcatcc tcgcgaatct ccttattgat tctggccagt
     4561 gcctgataca ccacacggcc ggcgccaatg ttgaggtggc ccacttccga attgcccatc
     4621 tggccctccg gtagaccgac gtcttcacca aaggtaatca atgtcgaatg cggatagata
     4681 gtccacaacc ggtcaaaatt gggtgtttgg gcattgtaaa tggcatcact ggaatgcacc
     4741 ggcccgatgc cccagccatc caaaatggtc aatattgttt tctttccttc catgatgtaa
     4801 aaataatcag cttttatcag aaactgtcaa atccactaaa attggccgga ttttgcagga
     4861 aaaatattgc gtcatctgtg atgaggcggg atggcggatg ttattcatga ttgtaggatt
     4921 ataacatggc tccgggaatc tattacctaa ttattatctt gagtctgtac ccctaaatac
     4981 aggcatccaa tgattgattt gcagtattaa aacaaagcaa cctcttctcc cgggggcctt
     5041 cgcgtcatcg aatattttcc taaaaatttc cacttattca ataaaacaaa gttttctcct
     5101 attataatat tggcataaaa tttgtgttat atatctaaag agaatcacaa aaaatggtta
     5161 gaacacttgc tttgttttat tttttgctcc tgactacact tgtgttcggg cagcagcgta
     5221 tcacgactgc catcaggaat ggtgatgcta aggccctgtc cggttttttt aataagcaag
     5281 ttgaattgac ttttgatgat cggggagatc ttatgactcg cgccgaagca gaaaggatgc
     5341 tcgagtcttt cttcaagacc aacaaggtca tttcctatac tccatctcac tccggaaaag
     5401 cccccgatca ttccggcagt tatacccttg gaaatatcag aacagaaaac cgtcacttca
     5461 ggatattcat taagttccgt ccaggcttag gtttggattc catcagagag gtccgcatca
     5521 attccttgtg acaaaaacga ggccttccac tacttaaata actatttccg gtaattggtt
     5581 tttcacgatg cacccaaaaa tgcagctgta atacccgaag actctgagat ttgattaata
     5641 gaggatcttc tttcaagtgg gtccgtacta catcatttga ccaacttcat tgcctacatg
     5701 acccaaacag aaaaccgggc aggtcaaagc aggtcaaggg atgggttatc tttcgtctga
     5761 tttcggacat acgttcagaa aagaggaaat ataactggtt ggccctgcat ttttttgatc
     5821 ccaaaaagaa taaatattaa cgaatcttaa tcaaatgttc atgcggctat aaacaactct
     5881 tgaacaacaa agaatatgct ataaaaacaa atattccatc attggtatat gtcaattttt
     5941 tacagattcc tatacatttt ttaaaaaaag acaacacgat tggaactttt gaccccaaat
     6001 ttcaatttga tattataatg atatcatttt aataatctat cactaaataa acatttgaaa
     6061 ccatgaaagg cgaatattat ataaagccca agaacggatt cctgttcctg ttgattttgt
     6121 tcctattgtt gggagcgaca atactacttc ctgtcatgtt cggtgattct gccatttcat
     6181 tatttgctat tgttccgggc atattgtttt tgcttgtcct gccgggattt aaggtcattt
     6241 accctaatga agcaattgct tttacttttt ttgggaagta tgcagggacc gccaaggaga
     6301 acggtttttt ttgggtcaac ccgttttact tgagtaaaaa gctcacacta cgagcacgca
     6361 acctgaatgg agacagacta aaagtcaatg acggggtcgg caatccaatt gaaattgctg
     6421 ctgttattgt atggcaagtg gctgatactt tcaaagccgc attcgaagta gacaattatg
     6481 tcaattatgt catggtccaa agtgaagccg ctgtacgagc cctggcaaac aaatatccat
     6541 atgaccagtt tgaggacgaa aacgctcaat taacactact ttccggagca gaaaaggtca
     6601 atgacgaact tgagcgagcc ttgcacgaga ggctggacca ggccggcatc agaatcatag
     6661 aagcaagaat cagccacctt gcctatgctc cggagatagc gggagctatg ttgcagcgac
     6721 aacaggcgat tgccattgtt gccgcaaggc aaaaaattgt agaaggagct gttggtatgg
     6781 tagatttggc gctaaccaag attaaggagg gcagcattat tgatctggac gatcatacga
     6841 aagccgcgat ggtcagcaac ctgatggtcg ttttgtgttc cgaccgtgcc gctcagccta
     6901 tcatcaatac aggcacgctg aatcattgat tttaaccata ccccaacccg gcaatgcctt
     6961 tagagggcag cgccttctat gcaaatatca tggcgcttta ccatggtatt gccgggttaa
     7021 ataaaattca gatcttgagt aaaaagccat tcatattaag gatagataag catacgcaaa
     7081 agcttctgga aaaatgggct gcggacgaat tccgtagcct aaacggccag atcgaatatc
     7141 tgttgcatag agccctggtc ctgtcacatc gttggaagga agaagagatt ccccctgaaa
     7201 aggaagagcc aaccaacgac aattcaaatt gtgattaact gtccatggcg caagagttcc
     7261 tctgcggggg gaattcccgg tttactatat tcacaagggt ggggaagagg ttgttgagcg
     7321 gtagtttcga agtgggtaga taaataataa tcacttcgcc tgacgctatg gagcttctgc
     7381 agagaatgct ccaaaaaaaa tgtcatttcg aggtggtttt tacaatgaaa ttatacagag
     7441 atccggtatc cactgatatt cagcctatta atcatctacc tagttcgttt tttgcaagtc
     7501 tttaccaacc accttgccgt attttgttga ctttgagagc caaaaaccca ctaaatattt
     7561 acaaactgta attaccggac cttgacaaac cttgcattgc cgagattttt tccctcttta
     7621 ttcctgacga gcaggaagta aataccttcg gacaaccccc cggtatctat tggctttatg
     7681 acgtcacctg ccataacaac ttttcctgta atatctataa tgtggtatgt ggcattatcg
     7741 gacatttctt cacttgcgcc tatattaagt acacttgaga caggattggg aaaaacataa
     7801 aaaccagttg ctttggactg tgtcgtggaa gatgttttcc aggcatcttg aaattttgcc
     7861 cgaatcatgg gaatgccctt gagcagcggc gatgctgtca gctcggtcat cggtttccaa
     7921 tctccggtcg acttgtaata ggtcaaggct tctcctccct gataattcct gtcaaacccg
     7981 accgggataa actgcccggt ggaagaaaca gaaaattgtt gccagcctat gtaaaaatca
     8041 cccggtggaa tatagagtgg caaaggctcc tgggtcagat cattgaccag cggataggtt
     8101 gtaaatccct gaagcgtatc gtagtatgta tccgcataga ccggacgttg cagctgatag
     8161 taataatccg gctcttcttt caagctgccg acccagatct taagattgaa caactgatgg
     8221 gagacatcat catataatct aggaaaatga aattgaacgg cacgaagtgt atctcctgta
     8281 tttaagcgaa acttgaccgc aatctgtgat tttacactgt tgttggcctc ggcggcgatg
     8341 ttgagctcag cactgccatc atcgtatgcc agaacatatc cgacttctgc ttcggaagtg
     8401 acggtattgt tttcttcgag aaaggatact tcctggtcct gatcaaaatg gtatttagtc
     8461 ctgaaaatca aaggcaattg acctgcggga acagaagcca cagaagcggc aatcgattgt
     8521 gggtcgaagg gattgtcaaa agcattgaag ccggcgggtg ggttcagctg tggcactgta
     8581 ttgacaagct gtccattgta tatttccgta ccagtagtta tttcctgtat ttccagccgt
     8641 ggatcaccca aatcttcagc aacttccagg aaatgattgt agagcctgat ttttgttgta
     8701 tctgccaact ccttatcctc gaaaccggac agctgccggt aaggtatcgc cgaataagtc
     8761 cttaatatcc tgttgggtgg atctgtaaag gcgatatcct gttggttaag gcccggcaac
     8821 tgattggcga taagtttgac ataatccagg tgccatgtac tatatacccc aagccggtaa
     8881 ttgtaattaa caaacctaaa ttgaaatccc ct
//
LOCUS       DAOQMC010000082         8892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3085_length_8892_cov_4.959489, whole genome shotgun sequence.
ACCESSION   DAOQMC010000082 DAOQMC010000000
VERSION     DAOQMC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3085_length_8892_cov_4.959489"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKV31_04715"
     CDS             complement(<1..182)
                     /locus_tag="PKV31_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNB92194.1"
                     /translation="MKIEIWSDIQCPFCYIGKRHFEQALDQFNHKDNLQIEWKSFQLD
                     PTIPEAMEQIPTVYQYL"
     gene            590..1981
                     /locus_tag="PKV31_04720"
     CDS             590..1981
                     /locus_tag="PKV31_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB92195.1"
                     /translation="MTIRKRDIIIIVFSVLLSNGIMYMIQPAGRPVSLSLSNGWSRFV
                     NNEFAQGSGSNFIKAAKIGTPAVVRITVRNKAIPIWDIGSLVSSGSGVVITADGYIAT
                     NHHVLAPGNLYEVTFNDGHKYKATLTGSDQSTDVAVLKIDRDDCPYLTMANSDSVQVG
                     QWVIAIGNPYQLNSTVTSGIVSAKGRTIDLLKGEYPLEYFIQTDAIFNEGNSGGALLN
                     EEGELIGINTAIFTRSGKFEGYSFAIPSNIVRKMVGDIISYGKVQRAVLGVGIQDITD
                     RLARRIGEPIGSGVYINKVNPDSPASEAGLRTGDIIISINGTNIRTYPALQEQVALYQ
                     PGDKVNIRYRRGGTIHIAEVELMPLKIESKKTVVRTDAPLKGIGLELRDDEGIPDPGI
                     IVQDVIPGSSAARAGIKPGFKISEINGVVVTTIDQFINQLSKNQYRILLRGIEPESGL
                     VTEYSIVKPVSGL"
     gene            2579..3001
                     /locus_tag="PKV31_04725"
     CDS             2579..3001
                     /locus_tag="PKV31_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB92196.1"
                     /translation="MNNNLKSNMTTSGLLITSMFIVLLTVTGLLIRPSEGVVNGKVVY
                     MDKTEVQDAVINVLAKNDLSLVSSQLTDDKGTFSIKGLAPGEYYISVQMEAQRQKVYG
                     PVFVAKGKQKVVIKPIIIPVQANASQAVVTASVITAAS"
     gene            3140..3769
                     /locus_tag="PKV31_04730"
     CDS             3140..3769
                     /locus_tag="PKV31_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNB92197.1"
                     /translation="MDISTIFKNNEAWITNKLRNDPDYFGKLSSGQSPQLLYIGCSDS
                     RVSAEEIMGLGPGEVFVHRNIANLVNNIDLNTQSVIDYAVRALEIPHLVVCGHYKCGG
                     VEAALRPQNLGLLNPWLRNIRDVYRLHEKELNSIENEDQRYDRFVELNVIEQCINLIK
                     IASVQEAYNKGLVTIHGWVMDLKTGRIIDLGIDIEKEMTHIKEIYDLGT"
     gene            complement(4176..5675)
                     /gene="zwf"
                     /locus_tag="PKV31_04735"
     CDS             complement(4176..5675)
                     /gene="zwf"
                     /locus_tag="PKV31_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNB92198.1"
                     /translation="MANNKIPTTVFVIFGATGDLTYRKLMPALYNLELDNMLPENFSI
                     LGVGRSELTKDNLMEKLKEGTVKFSRRAVDDAVWNRLCSNFQYINGDFADQNLYDRVN
                     SAILDLKTAAQDKLNVIIYLAVSPTFFGPITKGLASFPFIKKRDQTRFVFEKPFGHDL
                     KSALDLNKEIMSVLRENQVYRIDHYLGKETVQNILAFRFANSFFEPLWNKNYIEWIQI
                     SSLEQVGLEGRGGYYDNSGALRDMIQNHMLQLMCMIAMEPPINFNADEIRNKKADVLH
                     AVRKYEGNEVFQNTVRGQYSAGKVSDELIPAYRDEANVRKDSNTETFAAALMYIDTWR
                     WEGVPFYIRTGKNLSQKATTIAVKFKPAPSYSFPREATGNWQSNLLLFNINPRMDIHL
                     KFQAKVPGQLMQLSQVNMEFDFDKELSANTPEAYEHLIYDVLEGDATLFMRSDQVEAA
                     WQIITPILDAWQNTKALEFPNYTPGSNGPVKADELLARYGHTWLNPGNI"
     gene            complement(5704..7101)
                     /gene="gndA"
                     /locus_tag="PKV31_04740"
     CDS             complement(5704..7101)
                     /gene="gndA"
                     /locus_tag="PKV31_04740"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HNB92199.1"
                     /translation="MNTYSIGMIGLGTMGRNLLLNIADHGHTIIGYDKDATKADQVNA
                     IPGYDKISATSDITTFVSSLEKPARIILLVPAGPIVDYVINDLTPLLSEGDIIIDGGN
                     SYYKDTDRRYAALKSIGIHFMGMGVSGGEDGARFGPSIMPSGDKVAYDHVKQILDDVA
                     AKYKGMPCSKYMGNASAGHYVKMVHNGIEYGMMQLIAEAYDLMKNGLGYTNSRMADIF
                     EIWSRNKLEGFLVESAVKVLREKDTLTPMDLIDNIKDVARQKGTGKWTSQDALDLQVP
                     VPTIDLAVVARDLSGFEAERKAIHTLISGQKNKISGNITANELGKALRAAFLITYNQG
                     LHLIHAASTVYRYETSMVDVVNNWKAGCIIRSSLLTNLEKVYTNNPETALIISDPDIF
                     AIIRESQTALKKVAVFVIKNDFGASGFTSALTYLNMFSTERSPMNMVQGLRDLFGAHT
                     FERTDRPGSFHQQWK"
     gene            complement(7425..8822)
                     /locus_tag="PKV31_04745"
     CDS             complement(7425..8822)
                     /locus_tag="PKV31_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92200.1"
                     /translation="MDQNTDIKGSIEALKAIASEIQASDALATYQEEEDEDSYKELID
                     EFESRLMDIHVDVANHFPLQLEAFEREFIDPDLEGLLLPRLLGFSVLRGYHDKAYKYL
                     TPQDHFKNLLIAITGSPNFEFIKNRIGQTVQIGFALSSDIWITNLLEGIENKRVHQWL
                     LSQKLDKFYDPEVRKIAYVKYRNQFSSHNYFTAKIPTNMGELKLFYPSLKHFLFQRQR
                     LRLDNSNIKPAITTMLLNPIFSDTEEQHQILFILLNFFNLTGDDRESVKKLFNKLRKE
                     NKDSSDEYFDFLIEMYENRTILGPDSDKRVKDFLDTSIKDNILTYYELVDQLHSKGFM
                     HVDVIEAIKNYLNNYKGLSNEAEAIRQAVLRYFRQWIDNADVKDYPDYFELFKTFTIY
                     MDLFGNEHFNQQLEYSSLAYIRRLLKHYTDKRGKDYQDIKKFVQHTFTEVDFLKDKDA
                     VEMFKTKRKSKEIKK"
BASE COUNT         2458 a         1730 c         1899 g         2805 t
ORIGIN      
        1 agatactgat ataccgtagg aatttgttcc attgcttcgg gaatggttgg atccaactgg
       61 aaggatttcc actctatctg caaattgtcc ttatgattga attgatccaa tgcttgttca
      121 aaatgtctct tgccgatata gcaaaaagga cattgaatat cgctccagat ttctattttc
      181 atcgttcctg attatttatc gtattggaac actttaattg tcaaatggtt gtggtaggat
      241 attttcggtc cttttctact tggtatttta aaatcaaacc ttataatggg tctttataaa
      301 tgatgattga ttggctgaaa ctattgatga acagattgtt tcttttccgg ggatttaatc
      361 acttcctttg tttatttttg gggaattgta attgcgatgt ctatctcgta tgtcatccgt
      421 ggtaaaatat ttctcaaggt atttgaatga attttattat aattttgacc attgaatagg
      481 cggttttatt ggaatttgaa aagattcgag ctttccggga gcaacaaaat caggtgggat
      541 tcgttttatt tgaataaatt tggatgattt aaatcgttga ttatcattta tgaccattag
      601 aaagcgggat ataataataa ttgttttttc agtgctttta agcaatggca taatgtatat
      661 gatccaaccc gcagggcggc cggtttcact ttctttaagc aatggttgga gtcgatttgt
      721 caacaatgaa tttgcacagg gatcaggtag taattttatc aaagctgcca aaatcggcac
      781 acctgccgtc gtaagaatca cagtcaggaa taaggctata ccgatctggg atatcggttc
      841 tctggtttcc tccggttcag gggtggtgat tactgctgac ggctatattg cgaccaacca
      901 tcacgtcctt gcacccggta atctttatga ggtgacattt aatgatggtc ataaatataa
      961 agctacgctt acaggcagtg atcagtcgac cgacgtcgcc gtccttaaga tagacaggga
     1021 cgactgtcca tatctgacca tggccaatag cgactctgtc caggtgggtc agtgggtgat
     1081 tgccataggt aatccttacc agctcaattc aacggtgacc tctggcattg tttcagcgaa
     1141 aggacgtacc attgacttac ttaaagggga gtacccgctt gaatatttta ttcagacaga
     1201 cgccatcttc aatgaaggaa atagcggtgg ggcattgctc aatgaggaag gagagttgat
     1261 tggaattaac acggccatat ttacccgaag tggtaaattc gaaggctact ccttcgccat
     1321 tcccagcaac attgttcgaa aaatggttgg ggatatcatc agctatggca aggtgcagcg
     1381 tgcagtactc ggtgtcggaa ttcaggatat tacagatagg cttgcgcggc gaatcggaga
     1441 gccaattggt tctggagttt atatcaacaa agtcaatccg gattcgcccg cctcagaagc
     1501 cggacttcga accggggata tcatcatatc tatcaatggg accaatataa gaacctatcc
     1561 agccctccag gagcaagttg ccctgtacca accgggtgat aaagtcaata tcaggtacag
     1621 aaggggtggc acaatccata ttgccgaagt cgaattgatg ccacttaaga ttgaatcaaa
     1681 gaaaacagtg gttaggactg acgcccctct gaaaggaatt ggattggagc tgcgtgatga
     1741 tgaggggatt ccggatccgg gcattattgt gcaggatgtc atccccggta gttctgccgc
     1801 acgtgccgga ataaaaccgg gcttcaaaat cagtgaaatt aatggcgtcg ttgttactac
     1861 cattgatcag tttatcaatc agctatcgaa aaaccagtac agaatcttgc tgcgaggtat
     1921 tgagcctgag agtggtctgg tcactgagta ttccattgtc aagccggttt cagggctata
     1981 aactattatc attttctttc ctgtaattaa ttgaattgca tactcccggt gggtaggtaa
     2041 tgatcagtta tcaggtcctt cagattattg ttgctaatgt gtccatcctt ttcagggtgc
     2101 agcggggagt tttcatatga cctgggttga cgaataccaa taggtacttt ctttattttg
     2161 aaagagcaaa attcctcatc caaatttgag tcaaaatata attttgttat atgccataaa
     2221 actataatcc gatagaaatt taccaaaaat ttatcatatt gttattaata taaatataac
     2281 atataatata tatgtaaata tcagaatggc aataaaatga atcttgatat ttgaatattt
     2341 tgctttttat tccaaacaag atgcattaag acagcagacg tgtgagtgat ggcttatttg
     2401 caggtgtttt tatatttaaa ttttttattt caaaataata ttttaatgac gtaaaacacg
     2461 gaataacagt aatataaaga tttttttaaa aaaatatatt aataaataaa ctacttagtg
     2521 tcaaaatacg ttaactttgt ggaacaaaaa tttaatcact tacaaaatta tatttgccat
     2581 gaataacaat ttaaaatcca acatgaccac atccggtctt ttgattacct ccatgtttat
     2641 cgtcctattg actgtaactg gtctattgat caggccatcc gaaggggttg tcaacggaaa
     2701 ggtggtgtat atggacaaga ccgaggttca ggatgcagtt atcaatgttc tggccaagaa
     2761 tgacctttca cttgtttcgt cacaattgac ggatgacaag ggaacgtttt ccatcaaagg
     2821 tcttgcgccg ggtgaatatt acatttcagt acagatggaa gctcagaggc aaaaggtata
     2881 tggacctgta tttgttgcaa aaggtaagca aaaggtggtg atcaagccga ttatcattcc
     2941 ggtacaagcc aatgcatccc aggctgttgt tacggcttct gtgattacgg ccgcatcctg
     3001 atagttgcat ttgaaaaaat tataactacc atgttccctc aagcctgatg tgacactggt
     3061 agttatttct gttttagagg taatttggac tgagagttat acgtctgatt cgttatattt
     3121 gttgaataat ctctccttta tggatatttc gacaattttc aaaaataatg aagcgtggat
     3181 aacaaacaaa ttacggaacg atcctgacta cttcggaaag ctatcatccg gacaatctcc
     3241 gcagttatta tatataggtt gttctgatag tcgtgtcagt gctgaagaaa ttatgggact
     3301 tggtccgggt gaagtgtttg tacatcgtaa cattgccaat ctggtcaaca atatcgacct
     3361 gaatacccag tcagttatcg attatgctgt acgtgctctt gaaatacctc atctcgttgt
     3421 ttgcggtcat tataaatgcg gtggtgttga agctgccttg cgcccgcaaa acctgggtct
     3481 attgaatcca tggctcagaa atattcgaga tgtatatcgt ctccatgaaa aagaactgaa
     3541 ttcgatcgag aatgaagacc aacggtatga ccgattcgtt gagcttaatg tcattgaaca
     3601 gtgtattaat cttatcaaga ttgcttctgt gcaagaagct tacaacaaag gtcttgtcac
     3661 aatccacggc tgggtgatgg atctcaagac cggtcgcatc atcgatcttg ggattgatat
     3721 tgaaaaagag atgactcata ttaaggagat ttacgatctg ggtacctaag atggagtgac
     3781 tacctttacc cattcgaaaa acaccggtaa aagcttcctg aggatccttt catagtccct
     3841 tttggtagat ttcattaata tgcctgcctg taaagaaacc tgatttacct taacgaggtt
     3901 tgttggaaaa atacagaaat accatgaaaa attattttag ccggatacac tgaaggcagg
     3961 aaatatttat tgtctcatct ttacatcatc aaaagcacga taatttaatt cgatgtgatt
     4021 gaatcctctg aaatgcggag gatataacaa cagaaagggt gatgcttaat aaatttctaa
     4081 aagggaagcg gtaaaagtat cgtttccctt ttttcttgta tagcaggaac gtttttattt
     4141 tattgcctta ccgacttttt actcaggggt tatggttata tattgccggg attgagccat
     4201 gtatgaccat atctggcgag taactcgtct gccttgaccg gtccattgga gccgggtgtg
     4261 taattcggaa actcaagcgc cttggtgttt tgccaggcgt ccaatattgg tgtgatgatc
     4321 tgccaagctg cttccacctg atcagaacgc atgaataaag tggcatcccc ttccaacaca
     4381 tcataaatca aatgttcata cgcttccgga gtattggccg aaagctcctt gtcgaaatca
     4441 aattccatat tgacctgact aagctgcatc aactgacccg gcaccttggc ctggaactta
     4501 agatggatat ccatccgggg attgatattg aataacagga gatttgattg ccagttgccg
     4561 gttgcttccc tgggaaagct gtatgaaggt gccggtttga atttgacggc tattgtagtg
     4621 gccttttgcg agaggttttt acctgtcctg atataaaaag gtactccctc ccaacgccat
     4681 gtatcgatgt acatcaaggc ggcagcaaat gtctccgtgt tggaatcctt cctgacattg
     4741 gcttcatccc gatatgccgg aatcagttcg tcactgacct tgcctgctga gtactgaccg
     4801 cggaccgtat tctgaaatac ttcatttcct tcgtacttac gtacagcgtg cagtacatcg
     4861 gcttttttgt ttctaatctc atctgcattg aagttgatgg gtggctccat ggcaatcata
     4921 cacatcaact gtagcatgtg attctgtatc atatccctca aggcaccact attgtcataa
     4981 tatcctcctc ttccttccaa tcccacttgc tccagggatg aaatttgaat ccattcgata
     5041 tagttcttgt tccacaacgg ctcgaagaag gaattggcaa aacggaatgc caggatattc
     5101 tgaactgttt ctttgcccag ataatggtcg atgcgataga cctgattctc ccgaagtact
     5161 gacataatct ccttattgag atccagggca ctcttcaggt catggccgaa tggtttctca
     5221 aagacaaatc gtgtctgatc tcttttcttg ataaatggaa atgatgccag tcctttagtt
     5281 atcggaccaa agaatgtcgg ggagacagca agatatatga tgacatttaa cttgtcctga
     5341 gctgctgtct tcaggtcaag gatggctgag ttgaccctgt cgtaaagatt ctgatcggca
     5401 aagtcgccat tgatatattg aaagttggaa caaagccggt tccagactgc atcatcgacg
     5461 gcacgtcttg agaatttgac ggtaccttct ttcagttttt ccatcagatt gtctttggta
     5521 agttcggacc taccaacacc taatatcgaa aaattttccg gaagcatatt gtccagttcc
     5581 agattatata atgcaggcat cagtttgcga tatgtcaggt ctccggtagc tccaaagatg
     5641 acaaagacgg ttgtgggtat cttattgttg gccattcaga caattattta atattgataa
     5701 gtattatttc cattgctggt ggaaggatcc cggccggtct gtcctttcaa aggtatgtgc
     5761 accgaaaaga tctctaagcc cttgtaccat gttcattgga cttctttctg ttgaaaacat
     5821 attcaaataa gtaagggcgg atgtaaatcc ggatgctccg aaatcatttt taatcacgaa
     5881 tacagcaact ttctttaatg ctgtttggct ttccctgata atggcaaata tatcagggtc
     5941 actgatgata agagcggtct caggattatt ggtatatacc ttttcaaggt tggtaagcag
     6001 ggaagacctg ataatacaac cggccttcca gttgttgaca acgtctacca tggaagtttc
     6061 gtaccggtag acggttgaag ctgcatgtat cagatgcaat ccttggttgt aggtaatgag
     6121 gaaggcagct cgcaatgctt tacccaactc atttgcagtg atgttacccg agatcttatt
     6181 tttctggccg gatatcaagg tatggattgc ctttcgttct gcttcaaatc cactcaggtc
     6241 tcgtgccaca acagccagat cgatggtagg aaccgggacc tggaggtcga gggcatcctg
     6301 gctggtccat ttgcctgtac ctttttgcct tgctacatcc ttgatattat caattaaatc
     6361 cattggagtg agggtatcct tttctctaag tacttttacg gcagattcaa ccaggaaccc
     6421 ttcaagctta tttcgtgacc atatttcaaa gatatcggcc atccggctgt tagtgtatcc
     6481 caggccattt ttcatcaggt cataagcctc tgcgatcaat tgcatcatgc catactcaat
     6541 gccgttgtgc accatcttga cataatgtcc tgcagaagca tttcccatgt atttactaca
     6601 tggcattcct ttgtattttg cagctacatc atcgaggatt tgttttacgt gatcataagc
     6661 cactttgtcg cctgaaggca taatgcttgg tccgaaacgc gctccatctt caccgcctga
     6721 tactcccatg cccatgaaat ggattcctat tgatttcaat gctgcatatc tccggtcagt
     6781 gtccttatag taagaattcc caccatcgat gataatgtct ccttctgaaa gcaatggggt
     6841 aagatcattt attacatagt caacaatggg gcctgccggt accaggagga ttattctggc
     6901 gggtttttcc aggctggaaa caaaggtagt gatatccgaa gtggcagaaa tcttgtcata
     6961 acctggtata gcatttactt ggtctgcctt tgtagcatcc ttatcgtagc cgataatggt
     7021 gtgcccatga tcagcgatat tgagtaaaag attgcgcccc attgtcccca agccaatcat
     7081 gccaattgaa tatgtattca tgaatctagt cagttagatg tgttaaattc agaagtatga
     7141 atttgaattt tcgataagga aacaattaat tctatgcagg gtttattctg tattgatatt
     7201 tttcaaaaaa atttcctgta gattcaaatt tttctaaaat aagatagcat gggaagttaa
     7261 atggaaaaat agcgtttgga ggtatggtcg tcttgataca ggtgattttc caattattag
     7321 tcggataatg atattccttc cattcatcaa aggatggtga atgagggcat taaaaaagcc
     7381 gtcattgtca caatggcagc ttttaatttt atatagggat caggttattt cttgatctct
     7441 tttgatttcc gtttggtctt aaacatttcc accgcatctt tatcttttaa gaagtccact
     7501 tcggtaaagg tgtgctggac aaattttttg atatcctgat aatccttacc tctcttgtcg
     7561 gtataatgct taagcaatct tctgatatag gcaaggctgg aatactccag ttgttggttg
     7621 aaatgttcat tgccaaacaa atccatgtat atagtgaatg tcttgaataa ttcgaaataa
     7681 tcaggatagt ccttgacatc tgcattgtcg atccattgcc tgaaatacct caatactgct
     7741 tgtcgtattg cttctgcttc attagaaagg cctttataat tgttaagata attcttgatt
     7801 gcctcaatca catctacatg cataaagccc ttgctgtgaa gttgatcgac cagctcataa
     7861 tatgtcagga tattgtcctt gatggaagta tccaggaaat cttttacgcg cttatcacta
     7921 tcaggtccaa ggatggtcct gttttcgtac atctcaatca ggaagtcgaa atactcatcg
     7981 gaggaatcct tattttcctt cctaagcttg ttgaatagtt ttttgacaga ttctctgtcg
     8041 tcaccagtca gattgaagaa attaagcagg ataaagagta tttggtgttg ttcttctgta
     8101 tctgagaaga tggggttgag caacatggtt gtaatagccg gcttgatgtt gctgttgtcc
     8161 aatctcagtc gttggcgttg gaaaaggaaa tgcttcaatg atgggtagaa caacttaagt
     8221 tcacccatat tggtaggtat cttggcggtg aagtagttgt gggagctaaa ttggttacga
     8281 tacttgacat atgcaatctt tctaacttcc ggatcgtaaa atttatccag tttctgggaa
     8341 agcaaccatt ggtgtactct tttattttcg atgccttcca gtaggttcgt aatccagata
     8401 tcgctactca gggcaaatcc aatttggaca gtctggccga ttctgttttt gataaattca
     8461 aagttgggtg atccggtgat agctatcaga aggttcttaa agtggtcctg aggtgtcaga
     8521 tatttatatg ccttgtcatg atatcctctc aggacactga agcccagcaa gcgtggcaat
     8581 agaaggcctt ccaggtcagg gtcgataaat tccctttcga aggcttcgag ttgtaaagga
     8641 aaatgattgg ccacatcgac atgaatatcc atcagcctac tttcgaattc atcaatcagc
     8701 tctttgtaac tgtcttcgtc ttcttcttcc tggtatgttg cgagagcgtc cgatgcctgg
     8761 atctcggaag caatggcttt tagcgcttct atactgcctt tgatatctgt attctgatcc
     8821 atttatattt aaaattttgg gttaaaaaat aggaacctgg tagaattttt aggtcctacc
     8881 aggttcattt ca
//
LOCUS       DAOQMC010000083         8861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3101_length_8861_cov_5.496707, whole genome shotgun sequence.
ACCESSION   DAOQMC010000083 DAOQMC010000000
VERSION     DAOQMC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3101_length_8861_cov_5.496707"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="PKV31_04750"
     CDS             complement(<1..92)
                     /locus_tag="PKV31_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNB92201.1"
                     /translation="MFNKILIANRGEIALRIIRTCKEMGIKTVA"
     gene            complement(152..634)
                     /gene="accB"
                     /locus_tag="PKV31_04755"
     CDS             complement(152..634)
                     /gene="accB"
                     /locus_tag="PKV31_04755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNB92202.1"
                     /translation="MTYKEIQELIRQVAKSDLAEVKVKDNESEVTIRTRYYSEGKTSS
                     VTYSQAPSPVQQPAAMTQTVAPSTPKSEAEAPIETKSTGLKEIRSPMVGTFYRSPGPD
                     KAAYVKPGDTISAGTVVCIVEAMKLFNEIESEVSGTIVKVMVEDATPVEYDQVLFLVE
                     "
     gene            complement(678..1241)
                     /gene="efp"
                     /locus_tag="PKV31_04760"
     CDS             complement(678..1241)
                     /gene="efp"
                     /locus_tag="PKV31_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNB92203.1"
                     /translation="MADTGDIRKGLCIDYNNDIYVVTDFQHVKPARGAAFVRTTLKSM
                     TTGKSLENTWPSGHKLDVVRVERRKFQFLYKDDNGLNLMDNESYEQITISEDMVDSPQ
                     FLKESMDVDILYHAEKDIPLRVELPQYINVQVTYSEPGAKGDTATNASKPATIETGAV
                     IKVPLFINEGDVIKIDTLTGAYIERAK"
     gene            complement(1371..2354)
                     /locus_tag="PKV31_04765"
     CDS             complement(1371..2354)
                     /locus_tag="PKV31_04765"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNB92204.1"
                     /translation="MIRAGITGVEAYVPETVLTNADLEKMVDTNDEWIRTRTGIKERR
                     IFNSPGKGASDLALPAVLKLLETTNTRPEEVELVIVATVTADHTFPDTANILCDKAGI
                     KNAYGFDINAACSGFLFALTTAAQFVMTGRHKKVILVGVDVMSSIVDYTDRATCIIFG
                     DGAGAVMLEPVEDGTGLIDSVLKGDGAGRHVLHMKAGGSVKPASHETVDAREHFVYQE
                     GKHVFKHAVNGMASTVAEIMKRNDLSKEDVAWIVPHQANMRIITSVAEQLDFPIEKVM
                     VNIEKYGNTTSATIPLCLWEWKDKLHKGDNIILTAFGGGFTWGSTLIRWSI"
     gene            complement(2419..3393)
                     /locus_tag="PKV31_04770"
     CDS             complement(2419..3393)
                     /locus_tag="PKV31_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNB92205.1"
                     /translation="MLITEDTNIIIDHARKVFDAEIHCLEAVRENIDAHFAESVRAIL
                     DCQGRIVLSGIGKSAIVAQKITATLNSTGSPALFMHAADAIHGDLGIIQPNDIVILLS
                     KSGNSPEIKELIPYLKKMGNKIIAIVSNLHSVLAHDADIVLHIPVEREADPNNLAPTA
                     STTAQMAMGDALAVSLLMSRGFTEKDFARFHPGGNLGKQLYTVVGEVCRYNNKPSVPT
                     NAGIRDIIVTISAGRLGITAVEEDGKVVGVITDGDLRRMLEKDADTTSIMARDIMTEK
                     PKTIDSQALAINAMQRLKELNITQLLVMDGDKYAGVIHIHDLLREGFV"
     gene            complement(3718..7038)
                     /locus_tag="PKV31_04775"
     CDS             complement(3718..7038)
                     /locus_tag="PKV31_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNB92206.1"
                     /translation="MTETLTHTSAENIIERLSTDENDPRQMAEICDSLFQHLLDHATE
                     AFRMPFNTVFSQLAFITSQFKVEHTRTRALHYYRLNHLHHPDKSDEENLKIRRGILSL
                     LNDMVFGTKTERFADLQTYALRYARSPKNYSGVVREMRFSITEAHLEEGFFLGWNEDD
                     PDAIRKVVIDEASREALLTELMEDLNYFSFPMTVTLINVNISGNEYHPECIILLPDFL
                     IDVTAIAECYSYDGVNPRLHFIKKLMPSHPSDSLFLGLCANSFLDELVFRPNLDFKEL
                     IPRLFKISPLYLASLSDDKVIELVNQAKTHYHNILDFVRNYREGSREELQRALIEPSY
                     CSPTYGLQGRLDLYIDSEDNRSIIELKSGKLFRENKYGINQSHYIQTLLYDLLVRKNE
                     KSDKDLRAFILYSRLEGDRLRFAPPVRSLQLDALMTRNRILTMEYRFALIGANGNDPT
                     DVFGLCLELMREGQQGYIAQNLKFLHDTLNALTSVELKYLKAFTGFVAREYFTSKSGL
                     MRDRSTTGQAALWLNTAEEKAKDHLIMAGLLPESVEITHRDTLITFRKSPMTDLLADF
                     RIGDIVVLYAPDKEGNLRWEGRQLFRGTLVENNSFDVTIRLRARQNRKDLIFADRHWN
                     MEKDVIDSGITGQFRSLISWAASDVYNRKLLLGLIPPAKPERRIEIADNDISPYHREL
                     LENALSAPDYFLLWGPPGTGKTRYMVKNLIQLVIANTEKPVLVLAYTNRAVDELCNAI
                     ESISPEYCEKYIRIGSRYGTDPSFVGNLLDEKLLEISNRKQLLELLHTRRIIIGTASA
                     LLGKTELFSLLSFEWALIDEASQILDPQIIELLSYVPKFIMIGDHKQLPAVVTQHRSR
                     CLVTDREMNDCGIFDLSQSLFERLYLRAIQQGWDWAYGVLHRQGRMHSDIMTFPARHF
                     YDNRLETLFEHQIAQDFLRPATEAGHSGRKAELSRRRMLFFDVRQENFGFQKMNNAEA
                     TLIRGLLLDLHDLYRECGRRLEDTTIGIITPFRAQIVNISTLIESLPFKLDIIVDTVE
                     RFQGGARDIIILSMVVSSPSHAASIISANIEGIDRKMNVALTRARERLIMIGNQEVLE
                     QLDYYREFVQEYGVSAMN"
     gene            7672..7745
                     /locus_tag="PKV31_04780"
     tRNA            7672..7745
                     /locus_tag="PKV31_04780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7706..7708,aa:Pro,seq:ggg)
     gene            complement(7727..7882)
                     /locus_tag="PKV31_04785"
     CDS             complement(7727..7882)
                     /locus_tag="PKV31_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92207.1"
                     /translation="MLIGHCCILDLIGTWQASENIRRLIYKVMATAKEKGFDQNRSLL
                     TGRDDRI"
     gene            8106..>8861
                     /locus_tag="PKV31_04790"
     CDS             8106..>8861
                     /locus_tag="PKV31_04790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB92208.1"
                     /translation="MSNKFLLVLFITFVSNYVFGQKDEVLFTVNKTPVTVSEFKYIYE
                     KSNNKNADYSEKSLQESLDLYTRFKLKVAKARDLKMDTLPSLRSELDSYKRQLADSYL
                     MDKEINEKLAKELYDRMQTDARVAHLFIAAGTDDTIKPLLKINEIKVRLNKGDKFEDL
                     VKLYSEDESSKKNGGDLGFLAPMYPGGFYSLENLVYSLKKGETGGPVKTSAGWHFVKI
                     LDTRTARGEMEIAQILVRITKDFDEGFAKDRIDS"
BASE COUNT         2273 a         1975 c         2005 g         2608 t
ORIGIN      
        1 atagccaccg tcttgatacc catttcctta caggtacgga taatcctgag agcgatctca
       61 cccctgttgg caatcaatat tttattaaac ataagaacaa ttctgttgat gaatagatgt
      121 gaaggcgggt taaaataaat cccgaaatcc tttactcgac caagaaaagc acctgatcgt
      181 actctacagg ggtagcatct tcgaccatga ccttaacgat ggtaccggaa acttccgatt
      241 cgatttcatt gaacagcttc atcgcttcaa cgatacacac tacggtgccg gctgaaatgg
      301 tgtcgcccgg tttgacataa gctgctttgt caggtccggg ggagcgatag aaggttccaa
      361 ccatgggaga acgaatttcc tttagtccgg ttgacttcgt ttctatcgga gcttcagcct
      421 ctgattttgg tgtagaagga gcgacagtct gtgtcattgc ggccggttgc tgaaccggtg
      481 acggagcctg agagtaggta accgaactcg tctttccttc agaataataa cgggtcctga
      541 tggttacttc agattcatta tccttgactt taacttctgc cagatcggac tttgccacct
      601 ggcgaatcaa ctcttgaatt tctttgtagg tcatgtcaac aaatttgttt gttagggtta
      661 gcgggtgagt tccgttatta tttggctctt tcgatatatg caccggtcaa tgtgtcaatc
      721 ttgattacat cgccttcatt gataaacaat ggaaccttga tcacagctcc ggtttcaatg
      781 gtcgccggct tacttgcatt ggttgccgta tcacctttgg cgcccggttc tgagtacgtt
      841 acctgcacgt tgatatattg aggtaattct accctcagtg gaatatcttt ttcggcatgg
      901 taaaggatat ccacatccat actttctttc agaaattgtg ggctatcaac catatcttca
      961 gaaatagtga tttgctcata tgattcatta tccatcagat tgaggccatt atcatcctta
     1021 tataagaact gaaattttcg tctttccact cgcacaacat ccagcttatg ccctgagggc
     1081 catgtgttct ccagggactt tcctgttgtc atactcttaa gcgtggtccg gacaaatgct
     1141 gcgcccctcg ctggtttgac atgttgaaaa tccgtcacaa catagatgtc gttgttgtaa
     1201 tcgatgcaaa gcccctttct gatatctcct gtatctgcca taaattatag tttttaattc
     1261 gggcgcaaag ctataaaatt aatgcttttt tattatctcg ttaaaaaaaa ttgattggtt
     1321 cggttattta aatttatgag taagaaactt tatcgaaagc ttcatgctgt tcaaatagac
     1381 catcgtatca aggttgatcc ccaggtaaag ccaccgccaa atgcagtaag aataatgttg
     1441 tcccccttgt gtaatttatc tttccattcc cacaggcata gaggtatggt tgcggatgtt
     1501 gtatttccgt atttctctat attgaccatg actttttcaa ttggaaaatc cagttgctct
     1561 gctacggagg ttatgatacg catattagcc tgatgcggaa caatccatgc aacatcctct
     1621 tttgacaagt cgtttcgttt catgatttca gccacagtag aagccatgcc attgactgca
     1681 tgcttgaata catgcttacc ttcctgatat acgaagtgct cacgggcgtc gacagtttca
     1741 tgagaggccg gcttcacgga gcctcctgct ttcatatgca agacatgtct gcctgctccg
     1801 tcacccttta aaacagaatc aataaggccg gtaccatctt ccaccggctc cagcatgacg
     1861 gcacccgcgc catcgccaaa aattatacaa gttgctctgt cggtataatc tacaatggaa
     1921 gacatgacat ctacgccgac gaggatgacc tttttgtgcc gaccggtcat aacaaactgt
     1981 gctgcggtgg tcaaggcgaa aagaaatcca ctgcatgcgg catttatatc aaaaccgtag
     2041 gcatttttga ttcctgcttt atcacaaagg atattggcag tgtccggaaa tgtatgatct
     2101 gctgtcacgg tggccacaat taccaactcc acctcttccg gcctggtatt agttgtctcc
     2161 agcaatttca agacagccgg caatgcaaga tcactggctc ctttgccggg actattaaag
     2221 attcttcttt ccttgatgcc ggttcgggta cggatccatt catcattggt gtctaccatt
     2281 ttctcaagat ctgcattcgt gagaacagtt tctggcacat aagcctccac tccggtaatt
     2341 cctgctctta tcatatctat tattttgtct ttgacgttgt gaatttattg aaatttaatt
     2401 tctcggacat catttttatt atacaaaacc ttcccttagt aaatcgtgaa tatgtatcac
     2461 cccggcatat ttgtcaccat ccatgacaag cagttgtgtg atattgagtt ccttgagccg
     2521 ttgcatggca ttgatggcga gtgcctggct gtcgatggtc tttggctttt cggtcatgat
     2581 atccctggcc ataatagaag tggtatcagc atctttttcc aacatcctcc tcagatctcc
     2641 atcagtaata acgccaacga cctttccatc ctcttcgact gctgtgatgc cgagtctgcc
     2701 tgctgaaata gtgacaataa tatccctgat gccggcattt gttggtacac tcggtttgtt
     2761 gttgtatcgg cacacctctc cgacaacggt atatagttgc tttccgagat tgccaccggg
     2821 atggaaccgc gcaaaatcct tttcggtaaa gcctcgtgac attaacaggg acacggcaag
     2881 tgcatccccc attgccattt gggctgttgt gctggcggtt ggtgcaagat tgttgggatc
     2941 agcctctctt tcaacgggga tatgaaggac gatatccgca tcgtgagcta acactgagtg
     3001 cagattggac acgatagcaa tgattttgtt gcccatcttt ttcaggtatg gaatcagttc
     3061 cttgatctcc ggtgaattcc cgctcttgga taacaaaatg acgatatcat ttggttggat
     3121 gatacccagg tcgccatgta tcgcatcagc tgcatgcata aacagggcgg ggctgccagt
     3181 ggagttgagt gttgctgtaa tcttctgtgc gacgatggcg ctcttgccga taccggataa
     3241 gactatgcgt ccttggcagt cgagaattgc ccggacggat tcggcaaaat gcgcatcaat
     3301 attctcccta acggcctcca gacaatggat ctcggcatca aacaccttac gtgcatgatc
     3361 aataatgata ttcgtgtcct cggttataag cattacatgg ctatgagggt gcaaatatag
     3421 tcatagcttg tggcttttgt gaaaaaatac ctaatattgt tgggtgtcaa tgaccggacg
     3481 cttacgcgat ggcaaattat ctaatgcaac cgcattagga atgctttgta catcaaatga
     3541 cccaaaaact tcttccggat aacttctttt taacccagat taggcaattg atcccatgga
     3601 ttatgtatag ttcggagttg tttttatgct tgtttttgcc agattacggg agacgcactt
     3661 tcagtacaca ttttttccca actatctcta ttaatcagtt ttcattgtca cgtcacccta
     3721 attcattgca ctaacaccat attcctgcac aaattccctg taataatcca gctgctccag
     3781 cacttcctga tttcctatca tgattagtcg ctcgcgggca cgcgtgaggg cgacattcat
     3841 cttacggtca ataccttcga tattggccga tatgatcgaa gcggcatggg atggggagga
     3901 cacgaccatg gacaggatga tgatgtctcg tgccccaccc tggaagcgct ccacggtatc
     3961 cacgatgatg tcaagcttga agggaaggct ctcgatcagg gtagagatat tgacgatctg
     4021 agcccgaaaa ggtgtgatga tgcctatagt ggtatcttcc agtcggcggc cacattcgcg
     4081 gtacaggtca tgaaggtcca gcaggagtcc gcggatcagt gtagcttctg cattgttcat
     4141 tttctgaaaa ccgaaatttt cctgcctcac atcgaaaaac aacatccttc tccgggatag
     4201 ctctgccttc cggcccgaat gcccggcctc tgttgcaggc ctcaggaagt cctgtgctat
     4261 ctggtgctca aatagggttt ccagtctgtt gtcgtagaaa tgtcttgccg gaaaagtcat
     4321 gatgtcactg tgcatccgcc cttgccggtg tagaacacca tatgcccaat cccaaccctg
     4381 ttgaatggcg cgcagataaa gccgttcgaa gagagattgt gacaggtcga atatgccgca
     4441 atcattcatt tcccggtctg taaccagaca cctgctcctg tgctgtgtca ctactgcagg
     4501 caattgctta tggtcgccaa tcatgatgaa tttcggcaca taactcaaca attcaatgat
     4561 ttgtgggtcg agaatctgac tcgcctcatc gatcagtgcc cactcgaagg atagtaggct
     4621 gaacagctct gtcttgccca gcagtgcaga agcggtgccg ataatgattc tcctggtatg
     4681 cagtaattct agaagttgct ttcggttact gatttccagt aatttttcat cgagcaggtt
     4741 gcccacgaag gatgggtcgg ttccataccg cgaaccaata cggatgtatt tctcacagta
     4801 ttccgggctg atggattcta ttgcgttgca caattcgtct acggcccgat tggtataggc
     4861 aaggaccaac acgggttttt cggtattggc gatgaccaat tggatgaggt tcttgaccat
     4921 atatctcgtc ttacctgttc ccggtgggcc ccacagcagg aagtaatccg gtgcggacag
     4981 ggcattttcc aacaattcgc gatgataggg agaaatatca ttatcagcaa tctcaatccg
     5041 tcgttcaggc ttagcaggag gaatcaatcc cagcagcagt ttacgattgt agacatccga
     5101 agctgcccac gaaatcaggc ttctgaactg accggtaatg cccgaatcta tgacatcttt
     5161 ttccatgttc cagtgccggt cagcgaatat gagatccttc ctgttctgcc tggctctgag
     5221 ccggatagtc acatcgaagg aattgttttc gaccagggtt cctctaaaca attgtctgcc
     5281 ttcccacctc aaattaccct ccttgtcagg agcataaagg acgacgatgt ctcctatcct
     5341 gaaatcagcc agtaagtccg tcataggcga ctttctaaag gtgatcaggg tatcccggtg
     5401 ggtaatttct acactttccg ggagcagtcc tgccataatc agatgatctt tggccttttc
     5461 ctcagccgta ttcaaccaaa gtgctgcctg cccggtcgta cttcgatcgc gcataaggcc
     5521 tgacttggaa gtgaaatatt cgcgggcgac gaaacctgtg aatgctttga ggtatttaag
     5581 ttccactgag gtcagtgcat tgagggtgtc atgcaggaac ttcaggtttt gtgctatgta
     5641 cccctgctgg ccttcacgca tcaattccaa acacaagccg aagacatccg ttgggtcgtt
     5701 gccattggcc ccgatgaggg caaatcggta ttccattgtg aggattctgt tgcgggtcat
     5761 tagtgcatcc agctggagtg agcgtacagg gggagcaaaa cgcagcctgt ccccctcaag
     5821 cctgctgtac aggatgaagg cccgtaagtc tttgtccgat ttctcatttt tccggaccag
     5881 caggtcgtaa agaagggtct gaatgtaatg gctctggttg attccgtatt tgttttcgcg
     5941 gaaaagcttc ccggatttca attcgatgat ggaacggttg tcttcgctgt caatgtatag
     6001 gtccagacgg ccttgtaatc catatgtcgg actacaataa ctcggttcta tcagtgcgcg
     6061 ctgcaactcc tcccggctgc cttccctgta atttcgtaca aaatcgagga tgttgtgata
     6121 gtgtgtctta gcctggttga ctaattctat tactttatcg tcactgaggg aagccaggta
     6181 aagcggactt atcttgaata atcgggggat taactcttta aagtccagat taggccggaa
     6241 taccaattcg tctaagaatg agttggcgca aaggcctaaa aacaaagaat cggaaggatg
     6301 ggaaggcatc agcttcttga tgaaatgcaa cctgggattg acgccgtcat aactgtagca
     6361 ttcggcgatt gcggtaacat cgattagaaa gtcaggaagg aggatgatgc attcaggatg
     6421 atattcattc ccacttatat tgacattaat cagggtgacg gtcatcggga agctgaaata
     6481 gttcaggtct tccatcagtt cggtaagcaa agcttcgcgg cttgcctcgt cgataaccac
     6541 cttgcgtata gcgtccgggt catcttcatt ccagcccaga aaaaagcctt cctccagatg
     6601 agcctcggta atggaaaagc gcatttcccg aactactccg gaatagtttt tgggagaacg
     6661 ggcatagcgg agtgcatatg tctgcaggtc ggcgaatctc tcggtttttg taccaaaaac
     6721 catatcattg agcaggctca gtataccacg tcggatcttc aggttttctt catcagactt
     6781 atccggatgg tggagatggt tcagccggta gtaatgaagt gcccgggttc gagtatgctc
     6841 gactttaaat tggctggtta tgaaagcaag ctgtgaaaat actgtattga aaggcattct
     6901 gaaggcctct gtcgcgtggt ccagcaagtg ttggaacaga ctatcgcaga tttcggccat
     6961 ctgacgcgga tcattctcat ccgtcgaaag cctttcgatg atattttccg cgctggtgtg
     7021 ggttagtgtc tcggtcataa ttgtgaaaat aatatattaa ctggaaatta aattaaattg
     7081 aataaattac ccggatattg actccaccat ctctaatcat tgaaatttta ttaaggtaaa
     7141 atcctggctt gaaataaaaa cgagaaaata tccggataag cataacgggt aaatttgaaa
     7201 ttgtaaaatg cgaagctacg gaaattttat tgaaatgtat cttcgatttg aatcaatttg
     7261 ttattcatac caaaggttat aactatatct gaaatcattg atccccgcta attatgctga
     7321 gaaacaaatg atgttttcct tgtgtttatt atattttatc atgtacacag ctttacggga
     7381 tacttttgaa ctgttataaa acaaattgca atttacagag tgtttatgta taagttataa
     7441 agccgcattt gggggctgaa tactgcataa tattaaaatg atactattga gtcgtgccac
     7501 cggtactttg ggttggctat ctgtaaatga taatcagctg gcagagggta aggaattgaa
     7561 ctatacttca agggtgtgaa tgattgcccg ggtgcattat cattcactct attgtactga
     7621 ttggttagaa ttttcttgct tatagtggaa gatttattat tttcgcctca tcgggatgta
     7681 gcgcagtctg gtagcgtaca cgtctggggg gcgtgtggtc gcaggttcaa atcctgtcat
     7741 cccgacctgt tagaaggctt cggttctgat cgaagccttt ttcttttgct gttgccatga
     7801 ctttgtatat cagtctccga atattttctg atgcctgcca tgtgccaatg aggtcgagta
     7861 tgcagcagtg tcctattaac aaatctttat tatttgaatt tggcaaaaaa agtatctttg
     7921 cactcgccaa gtaagggcca aaatgcaagt gtagcaagga ttttaataaa acgatcttcc
     7981 ttttgctgtc tacaatggcc tgattggtcc ggtaatttta tgaatattca ggcgtttttt
     8041 tcgtttattg aaatagcttt gggaatccat gatattattt ttttcgatta accattacaa
     8101 ttcgtatgtc taataaattt cttttagtgc tttttatcac ctttgtctcc aactatgttt
     8161 ttggtcaaaa ggatgaagta cttttcacag taaacaaaac tccggtaacg gtcagtgaat
     8221 ttaaatatat ctacgagaaa tctaataata aaaacgctga ctattctgaa aagtcactac
     8281 aggagagcct tgatctgtat acacgtttca agctcaaggt agccaaggca cgcgacctca
     8341 agatggacac ccttccgtcc cttcgttcgg aactggattc ttacaagcgc caattggctg
     8401 actcctatct gatggacaag gaaatcaatg aaaaactggc taaggagttg tacgacagga
     8461 tgcagaccga tgcccgggtt gcgcatctct ttatagcagc aggaacagat gataccatca
     8521 aacctttact aaaaatcaat gaaatcaagg tcaggctcaa taagggcgac aaatttgaag
     8581 atctggttaa attatattct gaagatgaat catcaaagaa gaatggtgga gacctcggtt
     8641 ttcttgctcc gatgtatccc ggcgggttct actcattgga gaatctggtg tacagtctga
     8701 aaaaggggga gacaggaggc ccggtaaaaa cttctgcagg ttggcacttt gtcaagatat
     8761 tggatacacg aactgcacgc ggtgagatgg agattgcaca gattctggtg cggattacca
     8821 aggattttga cgaaggtttt gccaaagaca ggatagactc c
//
LOCUS       DAOQMC010000084         8855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3106_length_8855_cov_4.321250, whole genome shotgun sequence.
ACCESSION   DAOQMC010000084 DAOQMC010000000
VERSION     DAOQMC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3106_length_8855_cov_4.321250"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PKV31_04795"
     CDS             complement(<1..955)
                     /locus_tag="PKV31_04795"
                     /inference="COORDINATES: protein motif:HMM:NF023578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNB92209.1"
                     /translation="MPKASTTIEKQVSCSHCGTECTGDIRAADAVFCCQGCKIVFGLL
                     NEKGLCDYYEYNEHPGLTIRPIENNSKFDYLNNDEITDKLILFKNNDQSHVRFYLPQM
                     HCSSCLYLLEHLNKLNDGILNVRVDFVKREAFIVFDHNLLALSDLAGLLTSIGYEPHI
                     SLSGDDTEILKKADRKKIYKVGIAGFCFANIMMMSIPSYLAGGVEIERPIALFLRYGM
                     LILSIPVVFYSGAEFFISAWKSARERYLNIDAPIALAIAITFIRSLYEIFLGSGEGYL
                     DSLSGIIFFMLVGRLLQDQVYKSLSFDRDFRSFFPIAVRQIT"
     gene            complement(957..1442)
                     /locus_tag="PKV31_04800"
     CDS             complement(957..1442)
                     /locus_tag="PKV31_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92210.1"
                     /translation="MSVSIEKQCQLLDRQLYPAVDEAIDAALMNCSDQDSDPELQEHL
                     FTVLRIRNEFESLSNFERKLVFNTLIRAGKNNPAKNVGPTANIIELIRLIRYKDHKIE
                     GYIQSLEPVSRGKYPCLQKLYDLLSRLFTIEKTKLYDLVDQIIPLNSPVVFDNGSFNN
                     N"
     gene            complement(1653..3884)
                     /locus_tag="PKV31_04805"
     CDS             complement(1653..3884)
                     /locus_tag="PKV31_04805"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNB92211.1"
                     /translation="MNATNEIHFLGIRHHGPGCAKSVQAFLDEYKPDLILLEAPENTQ
                     ELLEKTDISTFKLPVAILLFKEGEAENNYVLPFAKFSPEWIAIQYAKATAVPIHAIDL
                     PLGVKFNYDDEQIEPEDVTFPIKNTVDAFRYLAQLQGIEDVEQWWEINFEQTSSPSEN
                     FKQIQSIIDAIRPAIEAGSDQYNKAREANMRQHIRNAVKSGLYKRIVVITGAAHLPFV
                     RDYKKFSAKEDKALFKPQSKAKLTCNWIPWSYQRLSSINHYDAGVQYPLYYEHIFEYG
                     ASAPVTITSSLSLHLRKKGYDISLAHTLESVNLAQTLANLRSTDIPGIDTIIEACSAV
                     YGLDQKFKTDELLKEGLIGDTLGEVPVNITNQALIVDFERKIKDCKLKKYTQDTKSHD
                     LTLDLRNPVQLEASRLIWQLRLLGITWGKEAKGRTSAKGSFNEHWSLKWKSEFLILLF
                     HQCLYGSSIDQAAAIKTKDELNRKDNVLFYLDILTPVINSQIADLTEFLLKQIENTLF
                     KQEEAESWMQLIPQLCGYFKYGSVRSLDLSQVKNLIEILVTRLTINFENILISIPEQD
                     LDSTLDTIIRLHHAILQTGEESLTLLWMGHIERLLYTFEIPAIFRGWITGVCLEKKII
                     DLDEALGLMTDEMSDRDNLEPSVAWLLGIMSSQVFTAFAVPSIVKIIDLWLADIDYER
                     FREILPGLRKAFSKNSTKLKASFRMHSGKEKPALDTWTREKIIDEDLTKIFSGYIKSF
                     IPT"
     gene            complement(3881..5377)
                     /locus_tag="PKV31_04810"
     CDS             complement(3881..5377)
                     /locus_tag="PKV31_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNB92212.1"
                     /translation="MRSLSVFILLMNVTTYSFTQCLSGDCEDGQGVLKFKSGTVYIGE
                     FSGGEFDGTGIMISANGDRFFGKWKDDMRTGYGKLLIGGGEQRYEGEFRNNKFEGYGT
                     FQYRDKSIYTGMWESSSVNGPGRFRTSGGDIVEGIWQNGNLTHRQDITELMKGKSKKL
                     RDCNLSNCNSGIGIFNYPDGSRWEGNMRNGKPTRLGVCYYSNGDIYLGEWLDDRPQGY
                     GVFYYNDGKKIESFWEAGKAVQLTEPQITESPITQTQFDKQVKIYSVIIGIGRYPDLP
                     MLKYTDDDAYLLYSFLKSPEGGAVPDDQVKILIDEAANRENIIKTLKTTFAEADANDV
                     ILLYFAGHGINGYYLPFDSDGYKNRISYDEIKRILESSQAKHKLCIADACYSGSLLAQ
                     RSAFGESLDLFYSTFEKTKGGTAFLLSSKQEETSLESTGMRQGIFSHYLINGLKGASD
                     SNKDRIITIKELYDYISYQVKKSTAFAQNPVIAGNYDNNMPVGVVRER"
     gene            complement(5651..7147)
                     /locus_tag="PKV31_04815"
     CDS             complement(5651..7147)
                     /locus_tag="PKV31_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNB92213.1"
                     /translation="MFNSPGKQSVVRILISVAGYIIGALSTLFIYPLDMKLHGLSTFL
                     LTASLMFAPFILVGSGAVAINNYNEFKTEDKRDNGFLGVIISMYLLGSLIFALLFFVG
                     GDWLSHFFESRDADYNKYFMYILPLTILQAGCALIDVYLNNFTKVIIPALFKEFLLKL
                     TLPLLIGLHILGYISIEHVVWGLVGNYVLALVLLLIYTAYLRKESFVINFSFLTGDRV
                     RRIMHYAFFAILSAFGNQLVLYIDTVMIKAILPDFKYVSIYNMHSSISNFINIPVIAI
                     ISVASPIVARYLSENDMGKLAGLYRSTSSIMLLLGTFLLMGILINMESVYAFMSNGDA
                     YSLGFMIIVFLGLTKIVDMGTGLNGQIIGYSQYYRINFYTLIVLGLSNAILNYFLIHT
                     MGVTGAALATLISMVLYNLFKLYYVRKKFGIWPFSNKDILIIMFAILSALAAYMAPNT
                     GNPYIQILIRTTLFTVLFWAAALYFNLSPRVTIIIKDNWKKFRKGLGK"
     gene            complement(7151..>8855)
                     /locus_tag="PKV31_04820"
     CDS             complement(7151..>8855)
                     /locus_tag="PKV31_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92214.1"
                     /translation="YSEETIRGKSLIDKPGKADDGLDKDYAFSWSLSIPESMTHVIPN
                     FMGGESSSFFVSDPESHSLRALQNMNNQELAQQLAQTTSKYFGQQPFTAGAWYWGITV
                     VLFFFLGFFSLRSWIRWWGLCSLIFLVLLSWGNHFSALNYFLFDYFPLFNKFRAVSMT
                     INLAHLVLMLIGFLGLREFLTMDQAGRMKALKMTMIPAGVILILGFYQGWLHTLTSPI
                     DEKLAKYPELISALYQDRAVAVRGDVWRTFFFIAAFFGLLYLFVKYQWNNVVALSVLA
                     ALIFIDLVTVDKRYLPNSKFVMQSDLNASEEPRPVDKEIMQDKQLSYRVADFTSNMFN
                     DAFLSNFHKNVGGYHAAKLAIYQDVIDKYLSNPRDYFHVYGMLNTKYIIGSGADNRLF
                     AQPNPEAMGNAWFAREVKWVDTPQQELDALGDTANIHNAVVNKSFKSSIKSDSYTPDP
                     AAKIEMTKYIPDHMTYTYEAASPQFVVFSEVYYPEKKGWHVYIDGKRKEGLVRTNYVL
                     RGLEVPEGKHTLEMKFEPESYYAGQKFGRIGSILLLLLLAGAIVTEFRDEKNKDKNVK
                     E"
BASE COUNT         2547 a         1811 c         1826 g         2671 t
ORIGIN      
        1 ctgttatctg tctgactgca atcggaaaga aggaacgaaa atcgcgatca aaggaaagtg
       61 acttatatac ctgatcctga agtaatcgac ctactaacat gaagaaaatg atgcctgata
      121 ggctgtctag ataaccttcg cccgagccca ggaatatctc gtacaggctt ctgataaagg
      181 ttatggctat tgccagtgct ataggtgcat cgatattcag atatctttcc ctggctgatt
      241 tccaggctga tataaaaaat tctgcccctg agtagaatac gaccgggatg gaaagtataa
      301 gcattccata tcggaggaaa agtgctatgg gccgctcgat ttctacacct cctgccagat
      361 aggatggaat gctcatcatc atgatgttgg caaagcagaa tcccgctata ccaactttgt
      421 aaattttttt acggtctgcc ttcttcagga tttctgtatc gtcacctgac aagctgatat
      481 gaggttcata accgatagat gtcagtaatc ctgccaggtc agataatgct aagaggttat
      541 ggtcaaatac aatgaaagct tctctcttaa caaagtccac ccgcacattg aggatgccat
      601 cattcagttt gttaagatgc tccaaaagat agagacaact actgcagtgc atctggggca
      661 gataaaacct gacgtgactt tgatcattat tcttaaatag gattaactta tctgttattt
      721 catcattgtt taggtaatcg aattttgaat tgttttctat agggcggatg gttagaccgg
      781 ggtgctcatt gtattcataa tagtcacaca aacctttttc gttgagcagt ccaaacacta
      841 ttttacagcc ctgacaacag aaaacagcat cggctgccct tatatctccg gtgcattcag
      901 ttccacagtg agaacagctg acttgtttct ctattgttgt acttgctttt ggcatgtcag
      961 ttgttgttaa atgaaccgtt atcgaaaaca accggagaat tcagaggaat gatttgatct
     1021 acaaggtcgt atagcttcgt tttttcaatg gtaaaaagcc ggcttaataa atcatatagt
     1081 ttttgcaaac acggatactt acctcttgat accggttcta gtgattggat atatccttca
     1141 attttatgat ccttgtacct gattagccgg atcaattcaa tgatatttgc ggtaggacca
     1201 acattttttg ccggattatt tttacctgcc ctgattaggg tattgaaaac cagttttctt
     1261 tcgaagttgg atagactttc gaattcattt ctgattcgca ggacagtaaa taaatgttcc
     1321 tgtaattcag gatcggaatc ctgatccgaa caattcatca atgctgcatc aattgcttca
     1381 tccacagccg gatataattg acggtcaagt aattggcact gtttttctat agataccgac
     1441 attgcaacga ttttcagtca aatgaatgta caaagatgct tcgcgccttt taagtaaaca
     1501 atgacttaag aaaggataaa acatgacaaa agtcatattt tatattgtat ttatatatag
     1561 ggctaagata taaataatat tcaatagatt ggccctggat ttaagcaata tttaaaaaac
     1621 tgaaattaat aaaaacaatt aaatgtaata ttttatgtag gtatgaatga tttgatatag
     1681 cctgaaaata tttttgtcaa gtcttcatct atgatttttt ctcttgtcca tgtgtccaat
     1741 gcaggtttct ccttgccgga atgcatcctg aagctggcct ttagcttggt cgagtttttt
     1801 gaaaaagctt ttcgaagtcc cggcaggatc tctctgaatc tctcataatc tatatctgca
     1861 agccacaaat caatgatttt cacaatggaa ggtaccgcaa aggctgtaaa aacctgactg
     1921 ctcatgatgc ccagtaacca ggcgacactc ggttcgagat tatcccgatc actcatttcg
     1981 tctgtcatca gaccaagtgc ttcgtccagg tcgattatct tcttttccag acagacacct
     2041 gtgatccagc ccctgaagat tgccggtatc tcaaaggtat aaagtagcct ttctatatgt
     2101 cccatccata ggagagtcag gctttcctct ccggtttgaa gaatggcatg atggagtcgt
     2161 ataatggtgt ccagggtact gtccaaatct tgttccggaa tacttatcag gatattttca
     2221 aagttgatgg tcagtctggt gactaaaatt tctatcagat ttttcacctg actaaggtcc
     2281 agagagcgaa ctgaaccata tttgaagtag ccacacaatt gagggatgag ttgcatccat
     2341 gactcagcct cttcttgttt gaatagtgtg ttttctattt gtttgagtaa aaattccgtt
     2401 aggtcggcga tctgggagtt gatgacaggc gtaaggatat ccaggtaaaa taatacgttg
     2461 tccttcctgt ttagctcatc tttggttttg atggcagctg cctgatcgat gctggatcca
     2521 tacaggcatt gatggaagag caggatgagg aattcagact tccatttcag actccagtgt
     2581 tcattaaaac ttccctttgc agaggttcgt cccttggctt ctttgcccca ggttattcca
     2641 agcagtctga gttgccagat gaggcgggag gcttccagct gtaccggatt ccgaaggtct
     2701 agcgtaaggt catgactttt agtgtcctgg gtgtattttt ttaacttaca gtccttgatt
     2761 ttacgttcga aatccactat taaggcctga tttgtgatat taacaggtac ttcacccagt
     2821 gtatctccaa tcagcccttc cttgagtagt tcgtcggtct tgaatttttg atccaaacca
     2881 tacactgccg agcaagcttc aatgatggta tcgatgcccg gaatatctgt acttcgcaaa
     2941 ttggccaaag tctgggcgag attgactgat tcaagggtat gtgccaagct tatgtcatag
     3001 ccttttttac gcagatgcag cgatagggaa gaagtaatgg tgacaggtgc agaggcgcca
     3061 tattcgaaaa tatgctcgta gtatagagga tattggaccc ctgcgtcata atgattaatt
     3121 gagctaagac gttgatatga ccacggaatc cagttgcagg taagtttggc ttttgattgc
     3181 ggtttgaata gtgccttatc ctctttcgct gaaaattttt tataatcgcg aacaaaaggt
     3241 aggtgtgctg cccctgttat cacaacgatt ctcttatata gtcctgattt gacagcattt
     3301 cgaatatgtt gtcgcatatt ggcctcccgt gctttattgt actgatcact gccggcttct
     3361 atagccggtc tgatggcatc aatgatggat tgaatctgtt tgaagttttc ggagggactg
     3421 gacgtctgct caaaattaat ttcccaccat tgttccacat cctcgatacc ttgtagttga
     3481 gccagatacc ggaaagcgtc aacggtgttt ttaataggaa aagtcacatc ttcgggttca
     3541 atctgctcgt catcataatt gaattttacc cctaagggaa ggtcaatcgc atggattggg
     3601 acagcggttg ctttggcata ttggatagca atccattcgg gagaaaattt ggcaaatggt
     3661 aatacgtagt tgttttcggc ctctccctcc ttgaacaata agatagcaac aggcaattta
     3721 aaggttgaaa tatccgtctt ttcaagtaat tcctgcgtat tttccggcgc ttccaatagg
     3781 atcaggtccg gcttgtattc gtccagaaat gcctgtacag acttcgcaca gcctggacca
     3841 tgatgacgga tgccgaggaa gtgtatttca ttggttgcat tcatctttcc ctaactacac
     3901 ctacaggcat gttgttgtca taattgccgg ctataaccgg attttgggca aatgctgtag
     3961 atttttttac ttgatagctg atatagtcat acagttcttt gatcgtgatt attctgtctt
     4021 tatttgaatc agaagcgcct ttcagcccgt taatgaggta atggctgaat ataccctgac
     4081 gcattcccgt ggattccagg gatgtttctt cttgttttga agatagcagg aaggcagttc
     4141 cgcctttcgt tttctcaaat gttgaataaa acagatcgag ggattcgccg aaagcacttc
     4201 tctgtgccaa aagactaccg gaataacagg catctgctat acaaagttta tgtttagctt
     4261 gactggattc gagtattctc tttatttcgt catagctgat acgattttta taaccgtcgg
     4321 aatcaaaagg gagataatat ccattaatgc cgtgacctgc gaaatacaac aggatgacat
     4381 catttgcatc tgcttcggcg aaggttgtct ttaatgtttt gatgatattc tctctgttgg
     4441 ctgcttcatc aataagtatt ttgacctgat cgtcaggaac agcaccacct tcagggcttt
     4501 tgagaaatga atacagcagg taagcgtcat catcggtata tttcagcatc ggtagatctg
     4561 gatatcggcc aattcctatg attactgaat atattttgac ttgcttatcg aattgggttt
     4621 gcgtgattgg actttcagtt atttgtggtt ctgttaattg aactgctttc cctgcttccc
     4681 aaaaggattc tattttcttt ccatcattat aataaaatac accatatccc tgaggtctgt
     4741 cgtccaacca ttctcccaga tagatgtcac cattagaata atagcaaaca cctaatctgg
     4801 tgggcttacc atttcgcata ttgccttccc atcttgatcc gtccggatag ttgaaaattc
     4861 ctatgcctga attgcagttt gataggttac aatcacgtag tttcttggac ttacccttca
     4921 tcaattctgt aatatcctga cgatgggtaa gattgccgtt ttgccaaatc ccttctacaa
     4981 tgtctccgcc agaagtgcgg aaacgaccgg ggccattgac agatgatgac tcccacatgc
     5041 ctgtgtaaat cgatttatct ctgtattgaa aggttccgta cccttcaaac ttgttattgc
     5101 ggaattctcc ttcatatctt tgttctcctc ctcctatcag gagcttgcca taaccggttc
     5161 gcatatcgtc tttccatttg ccgaaaaatc gatcaccatt ggctgatatc atgatgccgg
     5221 ttccatcaaa ttctccaccg gaaaattctc ctatatagac agttccgctt ttaaatttca
     5281 agactccctg accatcctca caatcacctg ataggcactg cgtaaatgaa taagtggtga
     5341 cattcattaa caggataaaa acagacaagg atctcatgat ttctttttgt atattcttct
     5401 gcgtaccgga gatcatattg gaataattat aatgttaatg aacgatagag tggtagaaaa
     5461 agttgctgat ttaaagtaga tgcttaacca gtgacataat aacgtttttg atgaattgaa
     5521 atattgtggg tatgggagaa tggcatatac attgtattgg ttgaagatta gtatgaatgc
     5581 cggtatttga tgttaataat gatctgatat tcaaatttta gaatagggaa taaatatttt
     5641 ttggagggaa tcatttgcct aaaccttttc taaatttctt ccaattatct ttaataatga
     5701 ttgtcacacg gggagataaa ttaaagtaca acgctgcagc ccagaaaaga acggtaaata
     5761 gcgtagttcg aatgagaatt tggatatagg gatttcctgt atttggagcc atataggccg
     5821 ctaatgcaga caggattgca aacattatga tgaggatatc tttgtttgaa aaaggccaaa
     5881 ttccaaattt tttcctgaca tagtacaact tgaagagatt gtaaaggacc attgaaatca
     5941 aggtcgccaa agcagctccc gtcacgccca tggtgtggat aagaaagtaa ttcaggattg
     6001 cgttggagag gcccagtaca atcagggtgt aaaaattgat ccgataatat tggctgtacc
     6061 ctatgatctg cccattaaga cctgtgccca tatccacgat ctttgttagt ccaagaaaca
     6121 caataatcat aaaccctaag ctgtaagcat ctccattgct cataaaggca tacaccgact
     6181 ccatattgat cagaattccc atgagcagga aggttccaag caataacata atagaagagg
     6241 tcgaacgata caggcctgcc agcttaccca tatcattttc ggaaagataa cgcgccacaa
     6301 tcggactggc aactgaaatg atagctatta cgggtatatt gataaaattg gatatgctcg
     6361 aatgcatgtt gtatatggaa acatacttga agtccggtag aatggccttg atcatgacgg
     6421 tgtcgatata cagtaccaat tgattgccaa aagcgctcag aatggcaaag aaggcgtaat
     6481 gcatgattcg tcttacacga tcacccgtga ggaagctaaa attgataaca aagctctctt
     6541 tcctgagata ggcggtataa atcaatagta aaaccaatgc caggacataa ttacccacta
     6601 aaccccatac aacatgctct attgagatat aaccaagaat atgcaggcct attaacaacg
     6661 gcagcgtcag cttcagtaaa aattctttga acagtgccgg aatgatgacc ttggtaaaat
     6721 tgttgagata aacatcaatc aatgcgcatc ctgcctgtaa tattgtgagt ggcaggatgt
     6781 acataaaata cttgttgtag tcggcgtccc tggattcaaa aaaatgactg agccagtcac
     6841 caccaacaaa gaataacaaa gcaaatatta agctgcctaa cagatacatg gatatgatta
     6901 cacccaggaa accattgtcc cgcttgtctt ctgtcttgaa ctcgttataa ttattgatgg
     6961 ctacagcacc cgaaccgact aagatgaatg gtgcaaacat cagagaggca gtcagcagaa
     7021 aggtgctgag gccgtgaagc ttcatatcca aaggataaat aaatagcgtg ctcagggctc
     7081 caataatgta gcctgctaca ctgattaata tccttacgac cgactgctta cccggagaat
     7141 taaacaaaag ttattccttt acgtttttgt ccttattttt ctcatctctg aattccgtta
     7201 caattgcccc tgcaagcaat agaagcagaa ggatggaacc gatccggcca aatttctgcc
     7261 ctgcataata ggattccggt tcgaatttca tctccagtgt atgtttgcct tcaggcactt
     7321 caagtcctct cagcacataa ttggtacgca ctaatccttc cttgcgctta ccatcgatat
     7381 acacatgcca tcctttcttc tcaggataat agacttcgga aaagactacg aattgtggcg
     7441 atgcagcttc gtaggtataa gtcatatgat ccggaatgta tttggtcatc tcaatctttg
     7501 ctgcaggatc aggcgtataa ctgtctgatt ttatggaaga tttgaaagac ttatttacca
     7561 cagcattgtg aatattggca gtgtcgccta atgcatcaag ttcctgctga ggtgtgtcca
     7621 cccactttac ttcacgtgca aaccatgcat tacccatcgc ttcgggattt ggttgtgcaa
     7681 aaagtcgatt gtcggcacca cttccaatga tgtatttcgt gttcaacata ccatatacat
     7741 ggaaataatc tcttgggtta ctaagatatt tgtcaatcac atcttgatag atggctagtt
     7801 ttgcagcgtg atatccacct acatttttat ggaagttgct aagaaaggca tcattgaaca
     7861 tatttgaagt aaagtcagcg acgcggtaac ttaattgttt gtcctgcata atttctttat
     7921 ccaccggcct cggttcttca gaagcattta aatcagattg cattacaaat tttgaattag
     7981 gcagatagcg cttgtccaca gtcaccagat caatgaaaat taaagcagct aagacagaga
     8041 gggcaactac attattccat tgatacttta cgaacagata tagcaaacca aagaaagctg
     8101 cgataaagaa gaatgtacgc catacatcac ctctgacggc aactgccctg tcctgataca
     8161 atgcggaaat taattcagga tactttgcta atttttcatc tatcggtgag gttagggtat
     8221 gtaaccatcc ttgataaaaa ccaagaataa gaataactcc ggccggaatc attgtcatct
     8281 taagcgcttt cattctgcct gcctgatcca tcgtcagaaa ctctctgagg ccgaggaacc
     8341 cgataagcat caggaccaga tgggccagat tgatggtcat tgagactgcc cggaacttat
     8401 tgaagagcgg gaaataatcg aaaaggaagt aatttaaggc tgaaaaatga ttaccccagc
     8461 tcaataagac taaaaatatc agggagcaaa ggccccacca tcgaatccag cttctcaagc
     8521 taaagaatcc aaggaagaaa aataaaacta cagtaatgcc ccaataccag gcaccagcag
     8581 taaagggttg ttgtccaaag tattttgaag tagtctgagc cagttgctgt gccagttcct
     8641 gattgttcat gttctgcaag gcacgcaggg agtgagattc ggggtcactg acaaagaaag
     8701 aagagctttc tccgcccatg aaattgggga ttacgtgtgt catggattcc gggatgctga
     8761 gtgaccagct gaaagcatag tccttatcaa gaccatcatc tgccttgcct ggtttgtcta
     8821 ttagtgactt accgcgtatg gtttcttcac tgtat
//
LOCUS       DAOQMC010000085         8848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3112_length_8848_cov_4.767201, whole genome shotgun sequence.
ACCESSION   DAOQMC010000085 DAOQMC010000000
VERSION     DAOQMC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3112_length_8848_cov_4.767201"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..1006)
                     /locus_tag="PKV31_04825"
     CDS             complement(308..1006)
                     /locus_tag="PKV31_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92215.1"
                     /translation="MTLIAKPFHHLFFIIAVTQSILLTSCGSYSSMQTAKTVETGTGE
                     IGLNLYYPYGVINAIAQVADEDKKNFTIPYIQATGKYGIMDRLDAGINLSTFGQIGLE
                     AKYQFYGDQQSIFAAATGLGFNTFFFYYYDFQIPLHLSVHPLENLGIYATPKYIGQFV
                     NSFGLANTYFDYGGISGGFIYGNRVQFCVDITAAFPLRKIYSIDVFPNLYNFGAGVKW
                     KIGGKTNKRSPYRF"
     gene            1224..1751
                     /locus_tag="PKV31_04830"
     CDS             1224..1751
                     /locus_tag="PKV31_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92216.1"
                     /translation="MRKLVFLLMVVATGLNAQDGIKTFRHEIKINTLNLLLGNPDLSY
                     EYLPDSRSGVGFFANINVDKNRLWDYDYMLGPYYRFYTGKKHASGFFIEGGVVVFGER
                     VVTGYYYDPETFTGHDIYVKEIGFGPSLAIGGKFIIAERTVFEFFGGLGRNFRGTEHG
                     SDIFSRFGISFGKRF"
     gene            2491..3378
                     /gene="tatC"
                     /locus_tag="PKV31_04835"
     CDS             2491..3378
                     /gene="tatC"
                     /locus_tag="PKV31_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNB92217.1"
                     /translation="MPLDQVDVDNMNTEEKEMTFFEHLEALRWHIIRSLIAVLVIATY
                     LFMQKDFLFKTIVYGPRDPGFFTYRLFCSFGEMMCIQPTHFDVITRDLAEKFTTHITV
                     SMVLGLIVAFPYVFWEFWRFVKPGLYPKERKSARGIVLVCSILFIAGVLFGYYFIAPF
                     ALSFLAGYELADTLATPTLNSYINYMIMFTLPVGIIFEMPVVSFFLTKAGVLSPGFLR
                     KGRRYALIIILIVAAILTPSPDVVSQLILATPLYILYEASIGVSARVYKGIEKRQKIE
                     EAELEAREKALLQKGRPLE"
     gene            3413..3850
                     /locus_tag="PKV31_04840"
     CDS             3413..3850
                     /locus_tag="PKV31_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92218.1"
                     /translation="MERYSSNLTLFYVVFLPVFSWTFLIFGVVGVFVGPIVNDDIYNS
                     IYIKMAFVVLLVVISIINIFFSLRLKRIEADDNRIYISNYFKHTTMSLDNIKSIRIRD
                     LWVMRIVRIRFHNKSMWGQSVLVLERENLLEKFCHAHGIAVES"
     gene            complement(3869..4981)
                     /locus_tag="PKV31_04845"
     CDS             complement(3869..4981)
                     /locus_tag="PKV31_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB92219.1"
                     /translation="MAQPRYRNIYVAATNQHIGKTTSTLGLVAAFLNRGLNVGYCKPV
                     GQQSLNFHDLIVDKDTILFADLIRFPIRQEVHSPVILGTGATTEFIDFPEKYNYPEKI
                     RYAQGILQEEHELTIYEGTGHPGVGSIVNLSNADVASMVNAGVIMVVDGGIGKTIDRL
                     NMSLSLFREKKVDLIGVIVNKVEETKIDKIRHYLGKAMSRMDLPLLGLMPYDKTLGYP
                     IMKSIMDAIKGEVLYNSDMLNNKVEDILAGSLMDVEQLKSDKGLLLVVSTGRVNEAIN
                     KLELISKIAGIRVSPLAGIVATGEGNINAKSIKYIEKNRIPLISTSLDTYGSVIKISR
                     IEVKINLNTPWKVQRAIELISNNVDLDAILDYATGK"
     gene            5167..5520
                     /locus_tag="PKV31_04850"
     CDS             5167..5520
                     /locus_tag="PKV31_04850"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNB92220.1"
                     /translation="MDQPFRKQDSVYSNKLRAGKKRTYFFDVKQTKGNDYYLTLTEST
                     RKFNSDSQERHKILVYKEDFNRFLAALQDTIDHIKTNLMPDYDFDEFARHQEELEKEY
                     EKRRASESTEDDMNW"
     gene            5670..6176
                     /gene="ribH"
                     /locus_tag="PKV31_04855"
     CDS             5670..6176
                     /gene="ribH"
                     /locus_tag="PKV31_04855"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNB92221.1"
                     /translation="MAKHTPLELPDLSEFVTEIKLGIVDTEWNPEIIRSMKVSARNTL
                     TEAGLSEDQWTELTVPGAFELPMGAKILLENGKYDAIICLGCVIKGETSHDQHINRSV
                     SSALMQLGLMSDIPIIFGVLTPNTEEQARERAGGSKGDKGREAAITALKMISLKFSFK
                     KHKKKIGY"
     gene            6493..8331
                     /locus_tag="PKV31_04860"
     CDS             6493..8331
                     /locus_tag="PKV31_04860"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB92222.1"
                     /translation="MSPLKFSLLALFCSFYFCVGAQQCYWQQHADYKIRVTLDASKHT
                     LAGVEDLVYTNNSPDTLYEVFYHLYFNAFQPNSQMDVRSRTITDPDGRVMDRISQLKP
                     HEQGEYKIRGVVQDKDKVKGVTVDGTILKVTLNKPIYPGEKTRLIMDYECQVPVQIRR
                     TGRYNKEGVAYSMSQWYPKMAEYDRNGWATDPYVGREFYGVWGRFEVFLTVPDSFKIA
                     GTGTPVSDTKSRKEKASVPSEYTMHHYVAENVHDFMWAADPHYRIDRITAAPGLDLVF
                     AYKDNPKVAETWKALQPIMKEAINYASERFGKYPYSRFSFIQGGDGGMEYPMATLVMG
                     ELSLNSLISVCVHEMMHSWYQGVLGFNESLYAWMDEGFTSYSEELVKEHLRSKGMYPG
                     KVSANPFERDAISMINFTLAGKSEPLSTHADHFTTNAAYSVASYVKGSLFLSQLGYVI
                     GEDALRSGLLEFYNIWMFKHPTGQDFMNLMEHRTGMELDWYYQYMINTTMLPDYAVDN
                     VKADGTGTAIMLGKKTQMPMPVDVTVEMKDGQKRFFTIPVDLMRMAKKTDQAMPGLTP
                     LKAWNWTNPDYFFHLDIPFAEIHSITIDDSNRMLDSDRKNNKWANE"
     gene            8427..>8848
                     /locus_tag="PKV31_04865"
     CDS             8427..>8848
                     /locus_tag="PKV31_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNB92223.1"
                     /translation="MRKVVSIVYPALLLLFLTSCKTSSAVSSYGSGTAVADTHVGAVS
                     NRKVHDVALNGKVYSAVWQQNAGEFKALCYQAYNAARQMVDQEKAKPHSKPLAIITDV
                     DETILDNSPYAVRQAELGNEFDIKSWTEWTAKGQAVAYP"
BASE COUNT         2558 a         1573 c         2016 g         2701 t
ORIGIN      
        1 aaaaaatcgt gtgaatgacc aaataagctc attgttgata ttatccaatt taagccccgc
       61 cccgttcaat atgaattcaa aaattgctgt ctttgtgttc atatccaagc tctgatcaca
      121 gaggatttat taatggcgtt tttctggaag gttatacaac tagaatgtga ttgctgccga
      181 tattcagatt tgtcagaggc ccggccaatg atttttatct catcgttcgg atttatacct
      241 gtgacgtaaa ttcgtttttt ttgaaatgcg aaattgatgg agtaggtaca tatggttttc
      301 cttttgatca gaacctgtat ggagaccttt tgttagtttt accgccgatc ttccacttga
      361 caccggctcc gaagttgtat agatttggaa aaacatcgat ggaatatatc ttgcgaaggg
      421 gaaaggctgc tgttatgtca acgcaaaatt gtacgcggtt accgtaaata aaccctccgg
      481 atatgccccc ataatcgaag taggtgtttg caagtccgaa agagttgaca aactggccta
      541 tatattttgg ggttgcatag atgcccaggt tctctaaagg gtgtaccgat aggtgaagag
      601 gaatctggaa atcataataa taaaagaaaa aagtattgaa gccaaggcct gttgctgcgg
      661 caaagattga ttgctgatct ccgtaaaact gatatttggc ttccagccca atttgaccga
      721 aggtactcag atttatacct gcatccaacc tatccatgat gccatacttt cccgttgctt
      781 gtatataagg aatcgtgaaa ttttttttgt cctcatcggc aacctgagca atggcgttta
      841 ttactccgta aggatagtat agattgagcc caatttcgcc tgtccctgtt tcgactgttt
      901 tggctgtctg catgctgctg tatgatccgc acgaagtcag aaggatggat tgggtgaccg
      961 caataataaa gaataggtgg tgaaaaggtt tggctattaa agtcatggta attatcttat
     1021 tagaattttg tatcaaaagt ataacgaaat atgaatagtc aggaaaggtt aaataaaacc
     1081 taaaagaaaa taaaaattat ctggtttagg ttattcaatg gaagatacaa taacataggc
     1141 ttgaaagagg aagctactaa tttgatgaat attgaatttt tggggtaaaa taatattatc
     1201 tttatataac aaaaatagaa tgtatgcgaa aactggtatt tcttttaatg gtggttgcta
     1261 ccggattaaa tgcacaggac gggattaaaa cattccgcca tgagattaaa atcaacacac
     1321 tgaatctatt gttgggcaat ccggatttgt cgtacgaata tctgccggat agtaggtcgg
     1381 gtgttggatt ttttgcaaat atcaatgtag ataaaaatcg tttgtgggat tatgattaca
     1441 tgttgggtcc ctattatcgg ttttataccg gaaagaaaca tgcgtccggg ttttttattg
     1501 aagggggtgt ggttgtattc ggcgaacggg tagttaccgg atattactat gatccggaga
     1561 catttaccgg gcatgatatt tatgttaagg aaattggctt tgggccttct ttggcaattg
     1621 gtgggaagtt tataatagca gagaggactg tatttgaatt ttttggaggg ttaggcagga
     1681 actttagagg aacagaacat ggatcagata ttttcagccg gtttggaatc tctttcggca
     1741 agcgttttta atataaaaat gccggaagat gattggcctt tggtgtgttg acgagatcga
     1801 tggattaaaa agtcagtaca ccgggttgga ttgttaactg gacaaatcga attggtaaaa
     1861 ttgattaata gctataagtc tattgatact aacttaggta cacattacta tatttataat
     1921 gtgtataaaa tgtttagaaa agtgttaatg agttgcctga aaataaataa aagaattatc
     1981 tttgtgtaaa agttacacaa ggtgacttga aagccaattg atgtattggt agcaaataaa
     2041 aaagaaaaaa taatttttat tatttgaaac ttttattata tttgcaatgt tataagtatg
     2101 acttgtgagt ttgcaagttg cttttcaaaa aagtatgaaa agttttccgg cgggaataga
     2161 aacacgccac aaacacgggg tgatttgaag ttttcataat tgggccctgt attcatctct
     2221 gagtacaggg ttttttttag aaacatcacc cggtggagat tccggcggga atagaaacac
     2281 gccacaaaca tggggtgatt tgaagttttc ataattgggc cctgtattca tctctgagta
     2341 cagggttttt ttattgccag attttttaat aagatgattc tattgaattt ccccgataaa
     2401 tattgtcttt tgcatgtttt tcaaattatt tgcaattagg gaatgcgcaa aactttacct
     2461 ttgccaggtt gataaataaa ggaattgaat atgccattag atcaggttga tgtagataac
     2521 atgaataccg aagagaagga gatgactttt tttgagcatc ttgaagcctt gagatggcat
     2581 attatccggt ccttgattgc agtacttgtt attgccacat atctcttcat gcagaaggac
     2641 ttcctgttca agaccattgt gtatggaccc cgggatcccg gtttttttac ctatcgcctg
     2701 ttttgctcgt ttggggaaat gatgtgtatc cagcctaccc attttgatgt tatcactcgg
     2761 gatctggcgg aaaagtttac cacgcatatt acggtttcca tggtacttgg acttattgtg
     2821 gcttttcctt atgttttctg ggagttttgg cgatttgtca aaccgggact gtatcccaag
     2881 gagagaaaat ctgcccgcgg catcgtattg gtatgttcta tcctgtttat tgccggggtt
     2941 ttatttggtt attattttat tgcccctttt gctctttctt ttcttgccgg atatgaattg
     3001 gcggatacgc ttgccacgcc aaccctcaat tcctatatca actatatgat catgtttacc
     3061 ctacctgtag gaattatttt tgaaatgcct gtagtttctt tttttcttac aaaagcgggt
     3121 gttttatcgc ccggatttct aagaaagggc aggaggtatg ccctgattat catattgatt
     3181 gtcgctgcca tcctcactcc atcgccagat gtggtctcgc agttgatcct tgccacaccc
     3241 ctctatattc tttatgaggc tagtataggt gtttcggctc gtgtgtacaa ggggattgaa
     3301 aaaagacaaa aaatcgaaga agccgagctg gaagcgcgag aaaaagcatt gcttcaaaaa
     3361 ggcaggccat tagaatagtg cttcttttcc ttacctttgc aaaaatcttt ctatggagcg
     3421 atactcttcc aatcttactt tgttttatgt tgttttttta ccggtttttt cgtggacatt
     3481 cttgatattt ggcgttgttg gagtttttgt aggtccaata gtcaatgatg atatatataa
     3541 ttcaatatac atcaaaatgg cctttgttgt actattggtc gtcatctcga tcatcaatat
     3601 tttcttttcc ttgaggttaa aaaggataga agcggatgat aacaggattt atatatccaa
     3661 ctatttcaaa catacgacta tgtctttgga taacataaag agcatccgga tcagggatct
     3721 ttgggtgatg aggattgtcc gtatccgatt tcataataag agcatgtggg gtcagtctgt
     3781 ccttgtactg gaaagagaga atttattgga gaagttttgt catgctcatg gtatagccgt
     3841 tgaaagctaa gtcctgaatc aggcttgatt atttcccggt tgcataatcc agaatcgcat
     3901 caaggtcgac gttattgctg attagttcta tcgcccgctg tactttccat ggtgtgttca
     3961 ggttgatttt gacttcgatt cttgagatct ttattacact gccgtaggta tcaagcgagg
     4021 tacttataag agggattctg tttttttcaa tgtatttgat actcttggca ttgatatttc
     4081 cttcgcctgt tgcaacaatt cccgccaggg gagatacgcg gatgccggca atcttactga
     4141 ttagctccag tttgttgatg gcttcattga cccggcctgt actcacgaca aggaggaggc
     4201 ctttgtcgga tttcaattgc tccacatcca tgagggatcc tgctaatata tcttcgactt
     4261 tattgttgag catgtcacta ttgtacaata cctcgccctt gatggcatcc ataatagact
     4321 tcataatcgg atatcccagg gttttatcat aaggcatcag accaagcaac ggaaggtcca
     4381 ttctggacat ggctttaccc aggtaatgtc ttattttatc aatttttgtt tcttccacct
     4441 tattgacaat cacaccaatc aaatctactt ttttctcccg gaatagtgaa agggacatat
     4501 tgagccggtc gatggttttg ccaattcctc catctacaac catgatgaca cctgcattga
     4561 ccatggaagc aacgtcggcg ttgctcagat tgacaatact ccctacaccc ggatgcccgg
     4621 tgccttcata gatggtcaac tcgtgctctt cttgcagtat tccctgagca tacctgattt
     4681 tttcagggta gttgtatttt tcagggaaat caataaactc cgtggtcgct ccggtaccca
     4741 gaatgaccgg gctgtggact tcctgtcgga ttggaaaacg aatcaaatca gcaaataaga
     4801 tagtatcctt atccacgatc aggtcgtgaa agttgagtga ttgttgtcct acgggcttac
     4861 aatatccgac attcaaacct cggttgagga atgcagctac cagccccagg gtggatgttg
     4921 tcttgccgat atgctggttg gtcgcggcga catagatatt tctgtaacgg ggctgtgcca
     4981 tacttttttt tctacaagta tataaatttc aattgaaatc gacaagatac tttttacaga
     5041 tacggaggct gaaaaaaggc aatctgcaat ttatactttt aaaaaataat gaattattaa
     5101 aaagataatt tataatcaaa taattatata tttgcttgaa ttatagaatt attaaaatta
     5161 aatacagtgg atcaaccatt tagaaaacaa gacagtgtct attccaataa actcagagcg
     5221 ggcaagaaaa ggacatattt ttttgatgtc aagcaaacga agggaaatga ttattacctg
     5281 acactgacgg aaagtaccag aaaattcaac tccgattctc aggaaagaca taaaatcctc
     5341 gtttataagg aagacttcaa cagatttttg gctgccttgc aggataccat tgatcacatc
     5401 aagacaaatc ttatgccgga ttatgacttt gatgaatttg caagacacca ggaggaattg
     5461 gaaaaagaat atgaaaaacg aagggcaagc gaatccacgg aagatgatat gaactggtaa
     5521 gcaagtgctc cggtattatt gttgcttttt ctccttactc gtttaaccgg acaccttgaa
     5581 cctgagggat tggaggaaat tagtaattgt aattgaacta accggcctat tgttcattat
     5641 caggataaat caatcgtata aatgtaatca tggcgaaaca tacaccactg gaattgcccg
     5701 atttatctga atttgttaca gaaattaagc ttggaattgt agatacggaa tggaatcctg
     5761 agattatacg ttccatgaag gtaagcgcca ggaacacatt gacggaagca ggtctgagtg
     5821 aggatcagtg gaccgaacta acagtgcccg gagcttttga attgcctatg ggtgcaaaga
     5881 tcctgctgga aaatggaaag tatgatgcca tcatttgtct gggctgtgtc atcaaaggtg
     5941 agacgagtca tgatcaacac atcaacaggt ctgtatcttc tgctctcatg cagttgggct
     6001 tgatgagcga tatccctatc atattcggtg tacttactcc aaatactgaa gaacaggccc
     6061 gggaaagggc aggaggctca aagggcgaca agggaaggga agctgctatt acggcattga
     6121 agatgataag cttaaaattc agttttaaaa aacacaagaa aaagattgga tattaagtac
     6181 aaatccaaat ggatggaatg tccttttgag tgtcttttcc gggagttatt gtccgaaggt
     6241 atttgtttta ggaaggaata atacaatcat gtgcaatgtg aatattgcag gggagacgat
     6301 aaaaatcccc gtgatgaaga agatgggtgg atgagagata aaggatagtg gccaggtagg
     6361 aaacaggtat ggcgaggagt gaaacgaaaa agagccaaat aatgattaaa cctgacattg
     6421 agttagtttc tatatttttg atcatcataa tttaaaattt ttccaacgaa atagctttta
     6481 atgaaattaa tcatgagtcc attgaaattc agtctattag ctttattttg ttctttttat
     6541 ttttgcgttg gggcgcagca atgttattgg cagcaacatg cagattataa gatacgtgtt
     6601 acgctggatg cgagtaagca caccttggcc ggggttgaag atttagtgta caccaataat
     6661 tctccggata ctctgtatga agtgttctat catctttatt ttaatgcctt ccagcctaac
     6721 agtcaaatgg atgttcgtag cagaaccatt accgacccgg acggtagggt catggacagg
     6781 atttcacaac taaaacctca cgaacagggt gaatacaaga ttcggggagt ggtgcaggat
     6841 aaggataagg taaaaggcgt tacggtggat gggacgattt tgaaagtaac cttgaataag
     6901 ccgatttatc ccggtgagaa gacgaggttg attatggact atgaatgtca ggttcctgtg
     6961 cagatcaggc gaactggaag gtacaacaag gagggtgtgg cttattccat gtcccaatgg
     7021 tatcccaaga tggcagaata tgatagaaat ggatgggcaa ctgatcctta tgtaggaagg
     7081 gagttttatg gagtctgggg tagatttgaa gtttttctta ctgtccctga ttcatttaag
     7141 attgccggta caggtacacc ggtgtctgat actaaaagca ggaaagagaa agcatctgtt
     7201 ccttccgaat ataccatgca tcactacgtg gctgaaaatg tccacgattt tatgtgggct
     7261 gctgatcctc attaccggat cgataggata acggcagctc ccggacttga tttggtattt
     7321 gcctataagg ataatccaaa ggtggccgaa acatggaagg cactgcagcc tataatgaag
     7381 gaagccatca actatgcatc agaaaggttt ggtaagtatc catattcccg cttttccttc
     7441 atacaaggag gtgacggggg gatggaatac ccaatggcaa cactggtcat gggtgagctg
     7501 agtctcaata gtctgatcag cgtatgtgtg catgagatga tgcattcctg gtatcagggc
     7561 gtgctgggtt tcaatgagtc cctatacgcc tggatggatg aaggttttac ttcgtattca
     7621 gaggagttgg tcaaggaaca tctgcgatcc aaaggaatgt atcccggaaa ggtttctgcc
     7681 aatccatttg aaagggatgc gatatcgatg atcaacttta ccttggcagg taagtcagag
     7741 ccgctttcga cgcatgccga tcattttacc accaatgctg cctattcggt ggcatcttat
     7801 gtcaagggat ccttgttctt gtctcaactt gggtatgtaa taggggaaga tgcattaagg
     7861 tctggcttgt tggaattcta caacatatgg atgtttaaac acccaaccgg tcaggatttc
     7921 atgaatttga tggagcatcg taccgggatg gaattggact ggtattatca atatatgatc
     7981 aacacgacaa tgttgcccga ctatgctgtg gacaatgtga aggcagatgg tacagggaca
     8041 gcgataatgc ttggaaagaa aacgcagatg cctatgccgg tggatgtaac agtagagatg
     8101 aaggatggtc aaaaacggtt tttcacaatt ccggtagact tgatgaggat ggctaagaag
     8161 acggaccagg ctatgcccgg acttacacct ctgaaggcct ggaactggac caatcctgat
     8221 tatttctttc atttggatat accgtttgcc gaaatccatt cgataactat tgatgattcc
     8281 aaccgaatgt tggatagtga caggaagaac aataagtggg caaatgagta gaaactttga
     8341 ttcaaaatcc tatctttgga caagaatcat taattattga ttgttattgg gagttgatcc
     8401 tcccccttaa attaagataa acgaatatga gaaaagtagt ttcaattgta taccctgcct
     8461 tgctgctttt gtttcttacc tcctgtaaaa cctcgtcagc agtatcttcc tatggttcag
     8521 gcactgcagt ggctgatacc catgttggtg ctgtgtccaa ccgaaaggtc catgatgtag
     8581 ctttgaatgg taaggtgtat tcggctgtgt ggcaacaaaa tgccggagaa ttcaaggcat
     8641 tatgttacca ggcttataat gcagccagac aaatggtcga tcaggaaaag gctaaacctc
     8701 attccaaacc attggccatc atcacggatg tggatgagac aatcctggac aattcgccct
     8761 atgcggtccg tcaggcagag cttggcaatg aatttgatat caaatcatgg accgagtgga
     8821 ctgccaaggg tcaggctgtt gcctatcc
//
LOCUS       DAOQMC010000086         8756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3165_length_8756_cov_4.225836, whole genome shotgun sequence.
ACCESSION   DAOQMC010000086 DAOQMC010000000
VERSION     DAOQMC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3165_length_8756_cov_4.225836"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PKV31_04870"
     CDS             <1..744
                     /locus_tag="PKV31_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HNB92224.1"
                     /translation="SILSEAMRQSMGRAACDAARACNYSGVGTVEFLVDDENNFYFLE
                     MNTRLQVEHPVTELITGLDLVEQQIRIAKGEKLSFVQEDLKINGHAIELRLYAEDSFN
                     NFLPSTGVLNEYIEPTGKGIRVDAGYRKGDEISVYFDPLIAKLIVWGEDRNLAIQVMK
                     AAIQQYQVSGLQTTLPFGAYVMNHPAFVTGNFDTGFVDKYYNSEEKAELEKSEAIVAA
                     IVAEYLSKDRHSVRPVHIERPSAWRINTW"
     gene            complement(909..2183)
                     /locus_tag="PKV31_04875"
     CDS             complement(909..2183)
                     /locus_tag="PKV31_04875"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNB92225.1"
                     /translation="MSGKYIDVIIGLQWGDEGKGKIVDYLAEQYDIVCRFQGGPNAGH
                     TLKIDGNKFVLHTVPSGIFRKNIINIIGNGVVIDPITLVKELDNLKAAGIAYKDRLKI
                     SRKAHLILPSHRLLDLASESSKGREKIGSTLKGIGPTYMDKTGRNGLRMGDLADPDFL
                     GKYQKLKEKHLALARQYDEVDFDLDGEENKWMECISQIKELAQIDCEYYINSALESGK
                     KILAEGAQGSMLDIDHGTYPYVTSSNTITAGVCSGLGVAPNKIGEVIGITKAYCTRVG
                     SGPFPSELNDESGEKLRSEGHEFGATTGRPRRCGWIDLPALKYSVILNGVTQLIMTKL
                     DVLNSFEKVYAAIAYEIDGQSTDQVPFNMETINRLTYKEYKGWNSSLENIRETDNLPV
                     EAWVFITDIEKRLGVPFSIISTGPEREKLILR"
     gene            complement(2328..3272)
                     /locus_tag="PKV31_04880"
     CDS             complement(2328..3272)
                     /locus_tag="PKV31_04880"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB92226.1"
                     /translation="MYPDLSYVFHDLFGTTPDNWLSLIKMFGLLLAIAILCSSYFLSL
                     EFKRRENEGSLHQLTVKKDGKTTKMWPHEKVGDITIIAAISGVVGAKIFAIFESIDSI
                     KSFLRDPIHTLFSGSGLAIYGGLIIAFIVVSRYVKRMGIKVLDMCDATAPSLMIGYGV
                     GRIGCQLSGDGDWGIVAGPKPDWFILPDWLWSQTYPRNVAEEGIKMAECAGKYCMELS
                     QGVFPTPIYETFFAFVIFGILWSLRKKLVISGQLFFLYCMLNGIERFFIEKIRVNDTF
                     DFGAFKASQAEIIAFILFLTGALGFYFVTWRSKKPIPE"
     gene            3497..4321
                     /locus_tag="PKV31_04885"
     CDS             3497..4321
                     /locus_tag="PKV31_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNB92227.1"
                     /translation="MRYTVILLVVMVLTSACNPAKKAKVHETVVVVPPSRDTVLIEHE
                     PEKVNRSDSAQIRSLVEGINMDFQSALLTGSMVFENTTDRYNFNFNIRMVKDSVIWMQ
                     LKKIGLEGARMLIRRDSLFLVDRIHRTYLKKSWNEIRTQLNGPVDFDALYSILTGNVE
                     YFDKGMDTLKTSENMKWIYSSDGNNRTVIGINAVFNTLVSYMVQDLRKDNLIRIGLAN
                     HKILYDKKYFSYLRDLEIFSNNAKLLYINFSFDEVRRNAVFNLPFEIPSSYKPMEM"
     gene            4350..5684
                     /locus_tag="PKV31_04890"
     CDS             4350..5684
                     /locus_tag="PKV31_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB92228.1"
                     /translation="MLIGVDQVIAQNTSELEKKKKKVEQNIKHTQDKLQKTKESKAST
                     LKDYNEIQNKIDSRKNIISGFQSEILTLQSSLQSKVRDISLLEERIDKLKSEYGRIMR
                     SAYKASLLTNEWMLILSSNSMNQAFNRWMYLRKIKKDRKAKANTIIAKQLELETELAN
                     LENIRSGKQVLLNKEQKQSTLLLNDLNKKNQLIASLNADEKKLIKTLNRQKKEQQAIT
                     REIENVIRKEIARKEKEAREKAERERKKEAERLAAEEARRNAGKKNESADASDISSEE
                     KSSEIAEKKVSAKVKEKKAIVVTETPESAALSSDFQSNRGRLPWPVKRGVIIRGFGTQ
                     AHPELSHVTIQNNGIDIKTDPDAAVEAVFNGEVVHIAFLSGYKNTVMVNHGKYYTIYS
                     NLESVGVSRGQKVKKGDLIGHAAHNADSGNTEVHFELWSKQSPLNPSLWLKR"
     gene            complement(5673..6071)
                     /locus_tag="PKV31_04895"
     CDS             complement(5673..6071)
                     /locus_tag="PKV31_04895"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNB92229.1"
                     /translation="MKKILVITSALGGLTVILGAFGAHGIKPLISEANIEVYKTAVSY
                     QFFHVLAIIGCGILYQLSGNKLFKIGSYAFLLGILLFSGSLYLLSTREATGLNSLAIL
                     GPITPLGGLSFITGWVMMMMGIIKEKKLSF"
     gene            complement(6072..7454)
                     /locus_tag="PKV31_04900"
     CDS             complement(6072..7454)
                     /locus_tag="PKV31_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB92230.1"
                     /translation="MTIAKNPMNYRKMLFPKIIDITRIQVDISILWIVLFVILLPGFS
                     HSQHSSKLKEYEVRVLEANLSKKADELNIQIAENNLNKGLAGLTPSVGLTAGDNISMT
                     SNITESATTVNKNGFRVNNSVNAGIFANMALYNGGRNKNLYERYKILIDQANLNQREL
                     AEQLIFQIRSLYYQAIRQNQLIKATEKAISYADQRLQLATNKLDIGKGNKVDVLQAEV
                     DRNQYASEIEMYKNDLDNIYVQMNALMNLPLGNRPEITDSSFSIQSIDKEEIRRSMLS
                     QHTEIIRNKINYNLSINKLQDAKGMWKPSLNASLGYSFSRSDNGGGFFRVNSSNGIAG
                     GVTLNVPIYDGKAIKTQIKNAELTTKQVEIQQEGLVLNLTTELDRYYNNYKNAMKLLE
                     IQQANVLNADENIKLGFSRFQAGLTDGFEFKQIQQSYTDANFRYIDILYQAKLNELQL
                     NYISGQILEN"
     gene            complement(7405..>8756)
                     /locus_tag="PKV31_04905"
     CDS             complement(7405..>8756)
                     /locus_tag="PKV31_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB92231.1"
                     /translation="DSIPELDVALSVTAPGFMGSGAPNTGFVRIRLVEPAFRIRTQQQ
                     VVDHVNRLVKRFTNAKVFVLQDQTISAGGGPRGGLPVSFIIQAPNFEKLEAIIPEFLN
                     KVNADPTFQGNDINLKFNKPELNVTIDREKAKTMGISVGDVAQVLQLAFSGQRYGYYI
                     QNGKQYSVIGQFDRINRDAPLDLKSLYLKTSSGQIVQLDNVVKVEELSSPPQLYHHNL
                     YQSATISAGLAPGKTIAEGIQAMDKIADQVLDESFSTDLSGASRDYAESSSNIMFALV
                     LALILVFLILAAQFESFIDPFIIMFTVPLAFAGAVFSLWYFNQTMNIFSQIGIIMLIG
                     LVTKNGILIVEFANQIRERGNSKMDAIIEGATARLRPIIMTSLATALGALPIAVAFGA
                     GAASRKGLGVVIVGGILFSLILTLFVIPALYSYFSRKKPYVVDHSKESHELQENVIS"
BASE COUNT         2605 a         1744 c         1823 g         2584 t
ORIGIN      
        1 agcatccttt cagaggcgat gcgccaatca atgggcaggg ctgcgtgtga tgctgcaagg
       61 gcttgcaact acagtggggt aggtacggta gaatttctgg tggatgacga aaataatttt
      121 tactttttgg aaatgaacac caggttgcag gttgagcatc cggtcacaga acttataacc
      181 ggtttggatc ttgtggagca acagatcagg attgcaaaag gtgaaaaatt gtcctttgtt
      241 caggaggatt tgaaaatcaa cggccatgca attgagctac gattatatgc ggaagattca
      301 ttcaacaatt tcctgccttc gacaggcgta cttaatgagt atattgagcc gaccggtaag
      361 ggtataagag tggatgcggg atatcggaaa ggagatgaga tttcagttta tttcgatcct
      421 ttgattgcca aattgattgt gtggggcgag gatcggaatc tggcgattca ggtgatgaaa
      481 gcggcgattc agcaatatca ggtaagcggc ttacagacaa cacttccctt tggtgcttac
      541 gtcatgaatc atccggcttt tgttacaggc aactttgaca ctggctttgt cgataagtat
      601 tataattctg aggaaaaagc ggaattggaa aaaagtgaag ctattgttgc agccattgtg
      661 gcagaatatt tgtcgaaaga tcgacattcg gtcaggccgg ttcatattga gaggccttca
      721 gcttggcgta tcaatacctg gtaggaagat tctaaagaca ttagattggc acccggtata
      781 gcggatgtat ctttaatagc tgatcataga taaaatggaa aaacacaatc ctcagaaatc
      841 caaaaacatg tataattctg tcaaggcata cttataagtg cctatttttg acagattctg
      901 agtcccattt atcttaaaat gagcttttcc ctttcgggtc ctgtagatat gatggagaat
      961 gggacgccca gtcttttttc aatatcagtt ataaaaaccc aggcctctac cggcaggtta
     1021 tcggtctccc taatgttttc aagggatgag ttccagcctt tatattcctt ataagttagc
     1081 cggttgatgg tttccatatt aaaggggacc tggtcggtag attgcccgtc aatttcgtag
     1141 gcgatggcag cgtatacttt ttcaaaactg ttcaacacat ccagtttggt cataatcagc
     1201 tgagttacgc cgttgagtat gacactatat ttcagagcag gcaggtctat ccagccacat
     1261 cttctgggtc ttccggtggt agccccgaac tcgtgtcctt cactgcgcaa tttttcacca
     1321 gattcatcat tcaattcaga aggaaagggg cctgatccga cacgggtaca gtaagccttt
     1381 gttataccta tcacttcacc gatcttattg ggagcaaccc ccaatccact acatacaccg
     1441 gctgttatcg tgttggaaga agtgacgtaa gggtaagtac catgatcgat atccaacata
     1501 gaaccctgtg ctccctcagc aagtattttt ttaccacttt caagggcgct gttgatataa
     1561 tattcacaat caatctgagc aagctcttta atttgactga tgcattccat ccatttattt
     1621 tcttcgccat ccagatcaaa atctacttca tcatactgtc tggctaaggc aagatgcttt
     1681 tcttttaatt tctgatattt acccaaaaag tccggatcgg ccaaatctcc catccttaga
     1741 ccgtttcgac ctgttttgtc catgtaggtc ggcccgatac ctttgagcgt tgatccgatt
     1801 ttctccctcc cttttgaaga ttccgacgca aggtcgagca gacggtgact cggcaatatg
     1861 agatgcgcct tcctgctaat cttcaatcgg tctttgtagg cgattcctgc cgctttcaaa
     1921 ttgtccagtt ccttaaccag cgttatcgga tcgattacca caccgttgcc gataatattg
     1981 atgatgtttt ttctgaaaat accgctagga accgtatgca aaacgaattt attgccatca
     2041 atcttgagcg tatgacctgc attcggcccc ccctgaaacc tgcatacaat atcatattgt
     2101 tctgcgagat agtcgactat ctttccctta ccttcatctc cccactgcag acctatgatt
     2161 acatcaatat atttaccgga catgtctcat gtttattatg gacaaaaata acaaatatta
     2221 ttacttttca tcaacttatg ttatacggat tgcaatacgg ggtaagttga tatatatatc
     2281 atttacttaa tatatgtaga tacttattta acagatagtt ggatttatta ttccggaatt
     2341 ggctttttac tgcgccaggt tacgaaataa aatcctaaag ctcctgtcaa aaacaggata
     2401 aaggctatta tttcagcctg ggaagctttg aatgcgccaa agtcgaaggt atcgttgacc
     2461 cggatttttt caataaagaa ccgctcgatt ccgttgagca tacaataaag aaagaataat
     2521 tggccggata tgaccaactt cttcctcaat gaccaaagaa tgccgaaaat tacaaaagca
     2581 aaaaatgtct catagatagg agtaggaaat acaccctgag acaattccat acagtatttt
     2641 ccggcacatt cagccatttt aattccttct tcggctacat ttcttgggta agtctgagac
     2701 cagagccagt caggaagaat aaaccaatcc ggttttggac cggctacaat accccaatct
     2761 ccatcaccgc tcagttgaca tccgatacgc cccacaccat atccgatcat cagtgaaggg
     2821 gcagtcgcat cacacatatc aagcacttta atgcccattc gtttgacata tcgtgaaacc
     2881 acaataaaag caatgattag tccaccatag atagcaagac cacttccgga gaaaagtgta
     2941 tgtatcggat ctctgagaaa agactttatt gaatcaatgc tttcaaatat tgcaaaaatt
     3001 ttagcaccca caactcctga tattgcagcg atgattgtga tatcgccgac cttttcatgt
     3061 ggccacattt tggtggtttt accatctttt ttcactgtaa gctgatggag agaaccctcg
     3121 ttttcccttc ttttgaattc caaactcaaa aaataggaac tgcatagaat tgcaattgcc
     3181 agcaaaaggc caaacatttt gatgagcgat aaccagttat ctggtgtcgt gccaaataag
     3241 tcatgaaaaa cgtaggataa atccggatac atagttattg tttttaatcg tcagaacgtc
     3301 ctcgagtcgt ccattaaatt gtaaaggtca ataaaattta tgatttcctt aatccatata
     3361 ttgtaatatt tttccaactt tggcgataat aattctgtag ggttattccg atgggaatta
     3421 ctctttctga ttaaatacca gatattgaac ggaaataata ttatggttat tgataatgta
     3481 ttttaaaacc aatcctatga gatacacagt tatattgctt gtggtgatgg tcctgacatc
     3541 agcctgtaat cctgcgaaga aagccaaggt acatgagacc gttgtcgtcg tgcctccctc
     3601 cagggatacc gtgttgattg agcatgagcc tgaaaaagtc aacaggtctg attctgcaca
     3661 gatccgaagt ctggtggaag ggatcaatat ggattttcag tccgccttat tgaccgggag
     3721 catggttttt gaaaacacca ccgatagata taatttcaat ttcaatatcc ggatggtaaa
     3781 ggatagtgta atatggatgc aattaaagaa gataggactt gaaggcgcca ggatgcttat
     3841 aagaagggat tccttatttt tggttgatag aattcatcgg acttatttaa agaaaagttg
     3901 gaacgaaatc aggacccaat taaacggtcc tgttgatttt gatgcactct attcaatatt
     3961 gaccggaaat gttgagtatt tcgacaaagg catggatacc ctgaagactt ccgaaaatat
     4021 gaaatggata tattccagtg atgggaataa caggactgta attggtataa atgccgtctt
     4081 caatactttg gtctcatata tggttcagga tttgagaaaa gacaatttga taagaattgg
     4141 cttagcaaat cataaaatat tatatgataa aaaatatttt tcttaccttc gtgatttaga
     4201 gatattttca aataatgcaa aactactata tatcaacttc tcgttcgatg aggtcagaag
     4261 aaatgccgta ttcaatctcc cgtttgaaat tccatcgtcc tacaaaccca tggaaatgta
     4321 gcatttcaaa aatcttcctt tgtattttct tgctgattgg agtagatcag gtaattgctc
     4381 agaatacttc ggagcttgag aaaaaaaaga agaaagtcga acaaaatatc aagcacacgc
     4441 aggataagct acagaaaacc aaggagagta aagcgtccac ccttaaagac tacaatgaga
     4501 ttcaaaataa aattgattcc aggaaaaata taatctccgg atttcagagt gagattctaa
     4561 cccttcagtc atccttacaa tcaaaggtcc gggatatatc attattggaa gaaagaattg
     4621 ataaactgaa atcagaatat ggcaggatta tgcgaagtgc ctacaaggct tctctgctta
     4681 ctaatgaatg gatgttgata ctttcgtcca acagtatgaa ccaggcattc aacagatgga
     4741 tgtatctccg aaaaatcaag aaagatcgaa aagcaaaggc gaacaccatt attgcaaaac
     4801 aattggaatt ggagactgaa ctggctaatc ttgagaatat caggtcaggt aagcaggtat
     4861 tgttaaataa ggaacagaaa caatctactt tactattaaa tgatttgaat aaaaagaatc
     4921 agttgattgc cagtctgaat gccgatgaaa agaaactaat caagaccctc aatcgtcaga
     4981 aaaaggaaca gcaagcaatt accagggaaa ttgaaaatgt catccggaaa gaaatagcta
     5041 gaaaggaaaa agaagctcgg gaaaaagcag agcgtgaaag aaaaaaggaa gctgaaagat
     5101 tagcagcaga agaggccaga aggaatgccg gcaagaagaa tgaatcggcg gatgcttcgg
     5161 atatatcttc tgaagaaaaa tcatcagaaa ttgcagaaaa gaaagtttcg gccaaagtaa
     5221 aagagaaaaa agcaattgtg gttaccgaaa cacctgaaag cgcggccttg tcttccgatt
     5281 ttcaatccaa tagggggcga cttccctggc ctgtaaaacg aggtgttatc atccgaggat
     5341 ttggtacaca agctcatcct gaactcagtc acgttacgat acaaaacaat ggaattgaca
     5401 tcaagacaga tcctgatgca gcggtagaag ccgtgtttaa tggagaagtc gtgcacatcg
     5461 cctttctgtc aggctataag aatacagtta tggtcaatca tggcaagtat tataccatct
     5521 attccaactt agaatcagta ggagtcagca gaggtcaaaa agtgaagaaa ggtgacctta
     5581 tcggacatgc agctcacaat gccgattcag gaaacacgga agttcacttt gagttgtgga
     5641 gcaaacaaag ccctctgaat ccttctcttt ggttaaaaag ataatttttt ttcctttata
     5701 atgcccatca tcatcatgac ccagcctgtg atgaaggaga gccctcccaa gggtgttatt
     5761 ggtccgagta tcgcaagcga gttgaggcct gtggcttctc ttgtggataa taagtataat
     5821 gagcctgaaa acagtaatat acctaggaga aaggcatagc taccaatttt gaacaatttg
     5881 ttgccgctca attggtatag aattccacaa ccaataatag ctaatacatg aaagaactga
     5941 tagctcacag ctgttttgta tacttcgata ttagcttctg atataagtgg tttgatacca
     6001 tgtgctccga atgcgcccaa aataacggtc aatccgccca gtgctgatgt aatgactaag
     6061 atttttttca tttagttttc gagtatttgt ccggaaatat aatttaattg taattcattc
     6121 aatttagcct ggtacaggat atcaatgtat ctgaagtttg catccgtata cgattgttgg
     6181 atttgtttaa actcaaaccc atcggtcagt cctgcctgga atcttgaaaa ccctaattta
     6241 atattttcat cagcgttcag gacattagcc tgctgtattt ccagcaattt catcgcattt
     6301 ttgtagttgt tgtaatatcg gtcaagctct gttgtgagat ttaaaacaag accctcttgc
     6361 tggatttcaa cttgtttggt tgtaagttct gcatttttga tctgcgtctt gatggctttt
     6421 ccgtcataga ttggcacatt taaggttact ccaccggcaa taccattgga tgaattaacc
     6481 ctgaaaaatc cacctccatt atctgatctg gaaaatgaat agcctaatga tgcattaagt
     6541 gatggtttcc acataccttt tgcatcttgc aatttgttga ttgacaggtt gtagttgatt
     6601 ttattccgta ttatttcggt gtgttgacta agcatggacc tgcgtatttc ctccttatcg
     6661 atggattgta ttgaaaaact agaatctgtg atttcaggtc tattacccaa aggaagattc
     6721 atcaatgcat tcatttgaac atagatattg tccaaatcat ttttatacat ttcgatttcg
     6781 gaagcatact ggtttctgtc aacctcggcc tggagtacat cgactttatt tccctttcca
     6841 atatccagtt tgtttgttgc caattgcaat cgttggtcag cataagagat tgctttttcg
     6901 gttgctttta tcaattgatt ttggcggatt gcctgataat aaagagatct gatttggaag
     6961 atcaattgct cggccagttc tctttgattc agattagcct gatcgatcag aatcttatag
     7021 cgctcataca gatttttatt ccggccaccg ttatatagtg ccatatttgc aaagatacct
     7081 gcattgaccg agttgttgac ccggaaacca ttcttattga cagtcgttgc agactctgta
     7141 atattagatg tcatagagat attatcacct gcagtcaatc caacagatgg agttaagccg
     7201 gccaggcctt tgttgagatt gttttccgca atctggatgt ttaactcgtc tgctttcttt
     7261 gacaaattgg cttccaatac tcttacttca tattccttta gttttgaaga atgctggcta
     7321 tggctaaagc ccggaagcaa gatcacgaat agtactatcc ataaaatcga aatatctact
     7381 tgtattcttg tgatatcaat gatcttagga aataacattt tcctgtagtt catgggattc
     7441 tttgctatgg tcaacaacat atggtttttt tcgtgaaaaa taggaatata gagccgggat
     7501 tacaaagagt gtcaggatga gtgaaaacaa aatccctcca acgataacga cacctaagcc
     7561 ttttctgctg gcagcgcctg ctccgaaggc aaccgcgatt gggagtgcac ctaaggctgt
     7621 cgccagactg gtcatgatga taggcctcag ccttgctgtt gctccttcga tgatggcgtc
     7681 catcttactg ttaccgcgct cccggatttg attggcaaac tctacaatca ggataccgtt
     7741 tttcgtaact aagccgatga gcatgataat tccaatttga ctgaagatat tcatcgtctg
     7801 gttgaagtac caaagagaaa aaacggcacc agcaaaagcg agcggtactg tgaacatgat
     7861 gatgaaaggg tcaatgaaac tttcgaattg tgctgctaat attaggaaaa ccaatatcaa
     7921 ggccaggacc agggcaaaca taatattgga cgaagattca gcgtaatctc tggaggcacc
     7981 gcttaggtca gtcgagaatg attcatccag cacctgatcg gcaatcttgt ccatggcctg
     8041 gataccttca gcaatagtct ttcccggggc gagtcctgcc gatatcgttg ctgactgata
     8101 gagattgtgg tggtatagct ggggcgggct gctcagctct tctactttga cgacattatc
     8161 caattggacg atctgtcctg aacttgtttt gaggtaaagt gatttcaggt ccaaaggagc
     8221 atcccggttg atacggtcaa actggccgat tactgaatat tgctttccat tctggatata
     8281 gtatccatat cgttgtcctg aaaatgctaa ttgtaatacc tgtgcaacat cgccaacaga
     8341 aatacccata gttttggctt tctcccgatc gattgtcaca ttcagttccg gcttgttgaa
     8401 tttcaggttg atatcatttc cctgaaaggt agggtctgca tttactttgt tgaggaattc
     8461 aggtattatg gcttccagct tttcaaaatt aggtgcctga atgatgaatg atacgggcag
     8521 gccgcctcgg gggccaccgc ctgcggaaat tgtttggtcc tgtaagacaa agactttggc
     8581 attggtgaag cgttttacaa gccgattgac atgatctacg acttgttgct gagtccgtat
     8641 tctgaatgct ggttctacaa gtctgatcct gacaaaaccg gtgtttggag cgccgctacc
     8701 cataaatccc ggtgctgtca ccgataaggc tacatccaac tcaggaattg aatcct
//
LOCUS       DAOQMC010000087         8630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3233_length_8630_cov_5.744373, whole genome shotgun sequence.
ACCESSION   DAOQMC010000087 DAOQMC010000000
VERSION     DAOQMC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_8630_cov_5.744373"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKV31_04910"
     CDS             <1..779
                     /locus_tag="PKV31_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92232.1"
                     /translation="KLVLDSITTIPGFELSDEKEWVERKTQMSKILTKEMMFTAFEPG
                     DYEFPAVPFFYDYQNTEKKGFSRPWRLKVLPMLNKSEDIAPNKDIVVVITFWDRFGVY
                     IIISLAVILLGVLSALLVSYLKRRSKYTPQAPVMQISPAQKARKELALLRKNELWKAE
                     DHKMFQTKLSLILRIYLQEAFDIPALKSTSHEIVSQLKKTGLPYNVIDTTDKALNIAD
                     LVKFAKAQSREEINFSFIGKVETLIDEAESFKGKIPDRYA"
     gene            772..1782
                     /locus_tag="PKV31_04915"
     CDS             772..1782
                     /locus_tag="PKV31_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB92233.1"
                     /translation="MLELGNIAFRFAWVLLFIPLLWWMFIILRRQRRTSGVYLPTAAA
                     VKDFVTLRTKLFRYLPWIKFAGLSFILISLAGPQRLEKNETINDSGIDISLVIDLSSS
                     MLARDFKPNRLEVTKTLAEQFVKERPNDRISVSVFAGEALAQCPLTYDHRMVVNILRN
                     LNAGALDDGTAIGMGLVSAVNTLKDSKAKSKVVILLTDGVNTAGYIDPLTAAKLAADY
                     HLRVYVIGVGSEGEAETPISRTPSGQVIYGYVPVEIDEPLMTEIANLTNGQYFRAKDE
                     NSLQQIYNRINLLEKTKIEKTVLKKRYDEYAWFLLIGIGLILTETILKNTWVRTPLFI
                     KY"
     gene            1783..3555
                     /locus_tag="PKV31_04920"
     CDS             1783..3555
                     /locus_tag="PKV31_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB92234.1"
                     /translation="MGQLTLQRPEYLLLLLVVPILGISMSLWRKRRRIIAADFAGKEV
                     YQKIAEPPKDNYRTRITLMLTALILAILALAGPSLPGPPEKTIKRASDIFLAIDVSRS
                     MQAQDLAPDRLTKTKLWAAELVQILKGQQIGLVVFAGAAYMYMPLTDDYNAAIDFING
                     ITTEMASYQGTAIGDAIETAQKAFGDETNKSRGLVIISDGEDHDSNAIQAAEKAAKKG
                     IVITTIGAGTEKGALIPEQDDLGFKYKLDEEGQPVRSKLNKEELIKIAAAAHGRYYSL
                     DNPRSALEGVKNMVDNLDKGTYDVHETSNNISLFPWLLWPAFFLFLIEMIMSMNISIK
                     TKSNNRTKVTTVTALMVFLLHNASYGQSAHTIMKEGNIAYQAKDYKKAAEHYDAAAKK
                     KPDFNSLYNEANALYQLGKYPDAAEKNMEAYQYAKTPQQKNMVTYNLGNSFFQTKDYE
                     KAIDAYKQALRNDPGDLDAKANLTRALVEKNKQDEQKKNNQKNQDKNQDQRQDQDQKD
                     NQNQNNQNQNNQNQQKNEPGNQAQNANQSNRQDKQNNQGKPQHLSPQEAESLLRIMKE
                     EEKNTRGKVMKRQQPEYNRSGKDW"
     gene            3679..6174
                     /locus_tag="PKV31_04925"
     CDS             3679..6174
                     /locus_tag="PKV31_04925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNB92235.1"
                     /translation="MERIKSIVRKMWIGLGVLVLFGFAYIIFLSLSGLPTFEELENPK
                     YDFASDIIASDNSVLGRLYIENRVPITYSQISPNIVKALIATEDVRFEKHSGVDPEAV
                     ARVIAKTVLLARKSSGGGSTITQQLAKLLYSDRDFSNMGRIRKSFALVNIKLKEWITA
                     IKLERRYTKEEIIAMYLNKFNFIYGAYGIEAAAETYFSKNNKDLTISEAAVLVGMLKN
                     PSLYNPVRRREQATLRRNTVLKRMYSNGILSESDYEKLSAEPIQLDFKPKTHIDGDAT
                     YFRMEVGKEVSQIIRKMDLQKSDGSLYDIYRDGLKIYTSIDADMQRIAEQVMLKHMKT
                     VQTNFWREWKGKDPWKYNADAKQLAIREASMKNLVRSSDRYINMRQNIFEQELDEINA
                     KYPEISFSDIEFDLWTAAEKEGLDKTAKRYRITDEQKRVYEQIMADPEYKITVAKWQQ
                     FRSTVRKAFNEKYKMKVFAYNAEGQKDTVMTPYDSIRYHRMFLQTGIVAIEPTTGQVK
                     VWVGGINHKYFKFDHIRTKRQVGSTFKPFVYASAIAFRGISPCMRVVDQQYTIEPGEA
                     SFGLIKPWSPGNAEGKFSGKSMTLYEALRESRNSVSVYLMKQLQSTDQVLELVNNMGI
                     DKSKIPAQPSICLGSADLTVLEMTGAYASFANNGTYVVPSFISRIEDKNGKVIYKNAS
                     DEKQALAPDYNYVMVDLLRYATRGSAGFQGIKSEVGGKTGTTNDYVDGWFMGITPSLV
                     VGTWVGGDDRWIHFNSLTYGQGSKMARPFFSEFIRQLELQKVSDYDPNARFIVPAGSE
                     NIVTDCSQYSNPNVIDQPIDTVKRKPGEDDFFQ"
     gene            complement(6358..8385)
                     /locus_tag="PKV31_04930"
     CDS             complement(6358..8385)
                     /locus_tag="PKV31_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNB92236.1"
                     /translation="MKKHVHIYDAAGNQLCCTLEQKINQKSDVTLVAAEPACCNHDHA
                     DNHVEEHDHIASVDGGWWHLFRPAIISFVLLISGIILDHYEPSFYDQWVRIGLYLLAY
                     IPVGWPVLKEAWESIAKGEVFSEFMLMGIATLGAMGIEQYPEAVAVMLFYSIGETFQT
                     LAVQRAKANIKMMLDQRPDEVTIVNGDKFETVKAATVPPGTIVRLNPGEKAALDGILL
                     SASGSFNTAALTGESVPATKFKGEEILAGMISVNESCRMEVSRPYEDSKLSGILRMVQ
                     DASSRKAPTELFIRKFAKVYTPIVVFLAVAICLLPFFFVSDYIFSQWLYRALIFLVIS
                     CPCALVISIPLGYFGGIGAASRNGILVKGGNYLDTLAGIRHVVVDKTGTMTRGNFEVR
                     EVEISEGFNRIEVLTDLKALQGFSTHPIAGAIQRYVQEEVVGIRVEDVLEIAGKGMSG
                     TVNGKHLLAGNLALMQVNKIPYDVSLEEIEYTLVAIAVDGKFAGYVTIADEIKPNAAA
                     VVRELADMGVELTMLSGDSNKVVAAVAREIGIRKAYGGLLPEDKVAKANEIKEASPPI
                     AFVGDGMNDAPVLALSDVGIAMGGLGSDMAIESADVVIQDDDIKKIPLSIRIGKKTKT
                     IVWQNILFAFGVKAAVLILGASGEATMWEAVFADVGVALIAVANAVRIQRM"
BASE COUNT         2618 a         2020 c         1870 g         2122 t
ORIGIN      
        1 agaagctggt tctcgactcc atcactacaa ttccaggctt tgaactaagt gatgaaaaag
       61 aatgggtgga gaggaagacg caaatgtcaa agatacttac aaaagaaatg atgttcacag
      121 ccttcgaacc gggggattac gaatttccgg ctgtaccatt cttttatgac tatcagaaca
      181 ccgaaaaaaa aggcttttcc agaccctggc gcctgaaagt actgcctatg ctcaataaaa
      241 gtgaagacat tgctcccaat aaggatattg tcgttgtgat taccttttgg gacaggttcg
      301 gagtgtacat catcatatcc ttggcagtaa tcttgctggg ggttctaagc gccttgttgg
      361 tcagctatct gaaacgaaga agtaaatata cgccacaagc gcctgtgatg caaatcagtc
      421 cggcacagaa ggccagaaag gaactggcgc tgttgagaaa aaacgaactt tggaaggccg
      481 aagaccacaa aatgtttcag acaaaacttt ccctcatcct acggatttat ttacaagagg
      541 cattcgatat acctgcctta aaatccacat cacatgagat agtatcccaa ttgaaaaaaa
      601 cagggctacc ttacaatgta atagatacaa cggacaaggc actaaatata gcagacctgg
      661 tcaaatttgc caaagcgcaa agcagggagg aaatcaattt ctcctttatt ggcaaagtgg
      721 agactctaat cgatgaggca gaatcattca aaggcaaaat tccggaccga tatgcttgaa
      781 cttggtaata tagcctttcg ttttgcctgg gtgctgttgt tcattccact gctatggtgg
      841 atgttcatca ttttgcgtcg acaacgaagg acttcaggtg tttatttgcc aactgcagca
      901 gcagtcaagg acttcgtcac cttgcgcacc aaacttttca ggtatttgcc atggatcaaa
      961 ttcgcaggtc tttcctttat cctgatatcg ttggccgggc ctcaaagact cgaaaagaat
     1021 gaaaccatca acgacagcgg aatagatatt tctttggtga tagacttgtc gagtagtatg
     1081 ctggctagag atttcaagcc caatcgcctt gaggtcacca agacgcttgc agagcaattt
     1141 gtcaaagaac ggccaaacga cagaatcagc gtttccgtct ttgcaggaga agcacttgca
     1201 caatgccctc ttacttatga tcatcgtatg gtagtcaata tcctgcgcaa cctcaatgcc
     1261 ggtgcattag atgatggaac cgccattggg atgggactag tgtcggcagt gaacaccctg
     1321 aaagattcta aagcaaaaag taaggtcgtc atcctgttga ctgatggtgt caatactgcc
     1381 ggttatatcg atccacttac tgcagctaag ctggctgctg attatcacct acgggtatat
     1441 gtgataggtg tcggcagtga aggtgaagca gaaacaccta tcagcaggac accaagcgga
     1501 caggtcatat acggttatgt acctgtcgaa atcgatgagc ccctgatgac agaaatcgca
     1561 aacctgacta atggccagta ttttcgggct aaggatgaaa attccttaca acagatttat
     1621 aacagaatca atctattgga aaaaaccaag atcgaaaaga ccgtgttgaa aaaaagatat
     1681 gatgaatacg cctggttcct attgataggc attggactga ttttgacaga gactatattg
     1741 aaaaatacct gggtgcgcac acctttattt attaaatact aaatgggaca attgacactg
     1801 caacgacctg aatatctcct cctcctcttg gtggtaccta tattggggat cagtatgtcc
     1861 ttatggagaa aaagacggcg aataatcgca gctgactttg ccggaaagga ggtatatcaa
     1921 aaaatagcag aaccaccaaa agacaattat cggacacgaa taaccctgat gttgactgca
     1981 ttgatcttgg ccatcctggc attggcagga ccaagtttgc ccggtccacc ggaaaagacg
     2041 atcaagaggg catcagatat tttcctggcc attgatgtca gccgaagtat gcaggcacag
     2101 gatttagcac ccgaccgact caccaagacc aaactttggg cggcagaact ggtccaaatc
     2161 ctcaaagggc aacagattgg ccttgtggta tttgcaggag ctgcttatat gtatatgcca
     2221 ttgaccgacg attataatgc cgccatagat ttcatcaacg ggatcacgac agaaatggca
     2281 agttatcagg gcacagcaat aggtgatgcg atagagaccg cacagaaggc atttggcgac
     2341 gaaacaaata aaagccgagg ccttgtcatc atttccgatg gggaggacca cgactccaat
     2401 gccattcagg ctgccgagaa agccgcgaaa aaagggattg taattaccac catcggagca
     2461 ggcaccgaaa aaggcgctct catccctgaa caggacgatc tgggcttcaa atataagttg
     2521 gacgaagaag gtcagcccgt tcgttctaaa ctgaataaag aggaactaat aaaaattgcc
     2581 gctgcagctc acggccgata ctatagcctt gacaatcccc gatccgccct ggaaggtgtc
     2641 aaaaatatgg tagataacct tgacaaggga acatacgatg tccacgaaac atcgaataat
     2701 atcagtcttt ttccatggct tctctggccg gcatttttcc ttttcctgat tgaaatgatc
     2761 atgagcatga atatttctat taagacaaaa agtaacaacc ggacaaaggt aacaactgta
     2821 accgccctga tggtcttcct gcttcataac gcctcttacg gccaaagtgc tcacaccatt
     2881 atgaaggagg gaaatatcgc ttatcaggct aaggattata aaaaagcagc agaacattat
     2941 gatgccgctg ccaagaaaaa gccggatttc aacagtcttt acaacgaagc gaatgccctg
     3001 tatcaactgg gtaaatatcc ggatgctgct gagaaaaata tggaagccta ccaatatgca
     3061 aagactcctc agcaaaaaaa tatggtcact tacaatctcg gcaactcatt tttccaaacc
     3121 aaggattacg aaaaggccat agatgcctac aagcaagcct tgcgcaacga cccgggagat
     3181 ctggacgcca aggccaacct gacccgtgcc ctggtagaaa aaaacaagca agacgaacag
     3241 aagaagaata accaaaagaa tcaggataag aatcaggacc aaagacaaga tcaggatcaa
     3301 aaagacaacc agaatcaaaa taatcagaat caaaataatc agaatcaaca aaaaaatgag
     3361 ccgggtaatc aagcccaaaa tgcaaatcag tccaatcgac aagacaagca aaacaatcag
     3421 ggcaaacctc aacacctcag cccacaggag gcggagtctt tgcttcgcat catgaaggaa
     3481 gaagaaaaaa atacccgcgg caaggtgatg aagcgccaac aaccggaata taaccgctcg
     3541 ggtaaggatt ggtgagcata tgcactatct cacggatgcc ttgccatatg gccatattac
     3601 atttccatgc tatatatttt tttcttcgta ctttcgtttt ataaaaacac actttttatt
     3661 ctgattatgg aagaaaacat ggaacgaata aaatcgatcg tcaggaagat gtggataggc
     3721 ctgggagtat tggtcctttt tggatttgct tatatcatat tcctatctct ttccggccta
     3781 cctactttcg aagaattgga aaacccgaag tatgattttg caagtgacat aatcgcttct
     3841 gacaactcgg ttctgggacg actgtacatt gagaacaggg taccaataac ctacagtcag
     3901 atctctccca atatagtcaa ggcgttgatt gcaactgaag atgtccggtt tgaaaaacac
     3961 agtggtgtag accccgaagc tgtggcaagg gttatcgcca agacagtttt gttggcccgt
     4021 aaaagctcgg gaggcggtag taccatcact caacagctgg ccaaattgtt gtattccgat
     4081 cgggatttca gcaatatggg ccggattaga aagtcgttcg ctctggtcaa tatcaagctc
     4141 aaggagtgga tcactgccat caagctcgaa cgccgttata ccaaggaaga gatcatcgct
     4201 atgtatctca ataaatttaa cttcatctat ggggcatatg gaatcgaagc tgccgctgaa
     4261 acatattttt caaaaaacaa caaggatctg accatatccg aagcagcagt cctggtaggc
     4321 atgcttaaaa atccatccct gtataatccg gtgcgaaggc gtgaacaagc aaccctcagg
     4381 cgcaataccg tcctcaaacg tatgtacagc aatggcatcc tgtctgagtc agactatgaa
     4441 aaactttccg cagagcccat tcaacttgat tttaaaccaa agacccatat cgatggtgat
     4501 gctacctact tccggatgga agtgggcaag gaagtatcac aaatcataag aaagatggat
     4561 ttgcaaaagt cggatggctc actttatgac atctacaggg atggattgaa gatctatact
     4621 tccatcgatg ccgatatgca acggatcgct gaacaggtca tgctcaaaca catgaagacc
     4681 gtccagacaa atttttggcg ggaatggaaa ggtaaagacc cctggaaata caatgctgat
     4741 gcaaagcagt tggccattcg tgaagcttcg atgaagaacc tggttcgctc atccgatcga
     4801 tatatcaata tgcgacagaa catattcgaa caagaactcg atgaaataaa tgcaaaatac
     4861 ccggagatca gtttttcaga tatcgaattc gacctttgga ctgctgccga aaaagagggc
     4921 ctggataaaa cagccaaaag atataggatc acagatgagc aaaaacgcgt gtatgagcaa
     4981 ataatggctg atccggaata taagatcacc gtagccaagt ggcagcaatt ccgatccacg
     5041 gtacgcaaag cattcaatga aaaatataaa atgaaagtat ttgcttacaa tgctgaaggc
     5101 cagaaagata ctgtgatgac accgtacgac agcatccggt atcatcggat gttcctgcag
     5161 acaggcattg tagccatcga accgacaaca ggccaggtca aggtttgggt gggcggcatc
     5221 aatcacaaat attttaagtt tgaccatatc cgtactaaaa gacaggtagg ctccacattc
     5281 aaacccttcg tctatgcctc tgctattgca tttcgtggca ttagcccctg catgcgtgtt
     5341 gtagaccagc aatataccat cgaaccggga gaagcgagtt ttggcctcat caagccatgg
     5401 tcacccggca atgccgaagg aaaattcagc ggtaaaagta tgacacttta tgaggcgctc
     5461 agagaatccc gcaactccgt atccgtctat cttatgaaac aactccagtc aaccgatcag
     5521 gttttggaac tcgtcaacaa tatgggtatt gacaagtcta aaatacccgc ccagccgtca
     5581 atttgtcttg gttctgccga tctcaccgtc ctggaaatga ccggagccta tgcatcattt
     5641 gccaataacg gcacctatgt cgttccgtcc tttatcagca gaattgaaga caaaaacgga
     5701 aaagtaatct ataagaatgc ttctgatgaa aaacaggctt tggcaccaga ctacaactac
     5761 gtcatggtcg acctacttcg atatgccact cgtggatcag caggatttca gggaataaag
     5821 agcgaagtgg gcggcaaaac cggtacaacc aatgattatg tcgatggctg gtttatgggc
     5881 attaccccga gcctagttgt cggtacttgg gtgggcggtg acgataggtg gattcatttc
     5941 aacagcctta catacggaca gggaagtaag atggccaggc cattcttttc tgaatttatc
     6001 cgccagctgg agttacaaaa agtttcggat tatgacccca atgcaagatt cattgtgcct
     6061 gccggatccg aaaatattgt gacggattgc agccagtaca gcaatcccaa tgttatagac
     6121 caacccatag acaccgtgaa aaggaaaccc ggtgaggatg atttctttca gtgatcgata
     6181 aaaaaataat tgctcctggc tgaaataacc caggttctgt ccattggaga ttttcaccgg
     6241 attcacaaac ccccgttttc ccaaccttag tgcggtgaaa tattgggtct ataatattga
     6301 tagtctgcca cttatatatt tttggtgcga caaagggaca atttttccaa gtctgcacta
     6361 cattcgttga atcctgacag cattggccac ggcaatcagg gcaactccga catccgcaaa
     6421 tactgcttcc cacatggtgg cttcaccgga tgcccctaaa atcaatactg cggccttgac
     6481 tccaaaggca aacaggatat tctgccatac gatagttttg gttttctttc caatacggat
     6541 ggagagcggg attttcttga tgtcatcatc ctgaatgacg acatctgcgc tttctatggc
     6601 catatcgctg cccaaacctc ccatggcgat gcctacatca ctgagtgcca ggacaggagc
     6661 atcgttcatg ccatcaccta caaaggctat tggtggtgat gcctctttga tctcattggc
     6721 tttggcaacc ttatcttcag gcaggagacc accataggct ttgcggatac caatttcccg
     6781 cgccacggct gccacaacct tgttactgtc gccactcaac atcgtcagtt ctacacccat
     6841 atcagccaat tcgcggacga ctgctgctgc atttggtttg atctcatcag cgattgtgac
     6901 atatccggcg aatttgccgt ccacagcaat tgccaccagc gtatactcga tctcttcaag
     6961 gcttacatca taaggaattt tattgacctg catcaaggcg agattgcccg cgagtaaatg
     7021 cttaccattg actgtgccgg acataccctt ccccgcgatt tccagaacat cctctaccct
     7081 gatgcctacg acttcctcct gaacatacct ttggatcgct ccggctatgg gatgtgtact
     7141 gaacccctgc agagccttta ggtcggtcag gacttcgatc ctgttaaaac cttctgagat
     7201 ttcaacctca cgaacctcaa aattaccacg ggtcatggta cccgtcttgt ccaccaccac
     7261 atgacgtatg ccggccagtg tgtccagata attacctccc ttgactaaga ttccgtttcg
     7321 gctggccgct cctattccac caaaataacc aagtggaata ctgatcacaa gtgcacacgg
     7381 acaggaaata actaaaaata tcaatgcgcg gtacaaccat tgactgaaga tataatcaga
     7441 gacgaagaaa aaaggcaaca gacaaatagc cactgccaga aacaccacta ttggggtata
     7501 taccttggcg aattttctta taaacaactc cgtaggggcc ttgcgtgaag aggcatcctg
     7561 taccatccgg agaatgccgg acaatttact gtcctcataa ggtctgctca cctccatcct
     7621 gcaagattcg tttacgctaa tcatccctgc caggatttct tcgcccttaa atttcgtggc
     7681 aggtacgctc tctcctgtca gtgcagcagt attaaaactc ccgcttgctg aaagcagtat
     7741 cccgtccaat gcagccttct cgcccggatt gagcctcaca atggtgcctg gtggcacagt
     7801 agctgccttg actgtctcaa atttatcacc attgacgatg gtgacttcgt cgggtctttg
     7861 atccagcatc atcttgatat ttgccttagc acgctggaca gcaagtgtct ggaaagtctc
     7921 cccaatcgag taaaaaagca tgaccgcaac agcttcaggg tattgctcta tccccattgc
     7981 tccaagtgtc gcgataccca tgagcataaa ttccgaaaac acctcaccct tggctatact
     8041 ctcccatgcc tccttcaata caggccaacc taccgggata taggcaagaa gataaaggcc
     8101 aattctaacc cattgatcgt aaaaagaggg ctcgtaatga tccaggatga taccggatat
     8161 caatagcacg aaactgatta tcgcaggtct gaacaggtgc caccatccgc catctacaga
     8221 ggcaatatga tcatgttctt ccacatgatt atctgcatga tcgtgattgc aacaggcagg
     8281 ctcagcagca accagtgtga catctgactt ctgattgatc ttttgttcca gcgtgcaaca
     8341 caactgattt cccgctgcat cataaatatg cacatgtttc ttcatatccg gattatttat
     8401 cggatgaata tcattacatt gaatgagaca aagatacgtc ttccattttc aacgattaaa
     8461 aaaatacatt ccttctcacc ctgattttgg tattcttttg catacaacaa tttattcttg
     8521 atattaatag cgttgtttga ttcagacaaa atgcaaatga ggcttaatag acaaaattaa
     8581 cccaagttgc gcttatgcgg atgaaattct gcgcagattg gaagtttccg
//
LOCUS       DAOQMC010000088         8567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3276_length_8567_cov_4.525493, whole genome shotgun sequence.
ACCESSION   DAOQMC010000088 DAOQMC010000000
VERSION     DAOQMC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3276_length_8567_cov_4.525493"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..618
                     /locus_tag="PKV31_04935"
     CDS             1..618
                     /locus_tag="PKV31_04935"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNB92237.1"
                     /translation="MPVDGKFSLPLLGYDYNALEPNIDAQTMEIHYSRHHQGYVNNLN
                     AAISGKPESSYSLEKICAMASNKDMAVRNNAGGHYNHSFFWKLLTPGGSNKPTGDLAS
                     AIERDFGSFDAFREKFNDAATKRFGSGWAWLVKGANNKLYVYSSPNQDNPLMTSLGYK
                     GIPIIGIDVWEHAYYLKYQNKRGDYIKNFWNIVNWDVANKNYSEK"
     gene            703..787
                     /locus_tag="PKV31_04940"
     tRNA            703..787
                     /locus_tag="PKV31_04940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:737..739,aa:Ser,seq:tga)
     gene            complement(1413..2807)
                     /locus_tag="PKV31_04945"
     CDS             complement(1413..2807)
                     /locus_tag="PKV31_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNB92238.1"
                     /translation="MNHTYHDLVNQTFYFPQEGFGLEDNKLLFHGIKLYELIEKYGTP
                     LKLVYLPKISDQIKKAKNFFDTAIFQLQYTGKYNYTYCTKSCHFRYVLEEVLKNDVHL
                     ETSSAFDIDLIWKLYADGLLHKDKIIVNNGFKNDAYIRRICGMIQSDFTNVLPVMDNW
                     EELDKFSQYLGDREFNVGIRVATEEEPHFEFYTSRLGIRKSEILNFYHEKIAQRPNIS
                     LKMLHFFVDTGIKDNVYYWDELRKGIKAYCELKKACPTLEAINIGGGLPIKNSLGFEY
                     DYGYMILEIVRQIQDACAENGIPEPDIYTEFGKYTVGESGAHFFSVIGQKQQNDNECW
                     YMVDNSLMNTLPDAWGINEHFILLPINHWDHEYKRVNIGGLSCDNSDYYNSEAHSNQV
                     YLPAVGKEEKLYLGFFHSGAYQDALSGYGGIKHCLLPSPKHVLIDRNPEGELVDWVWA
                     NDQDSESMLKILGY"
     gene            complement(2843..4240)
                     /gene="lpdA"
                     /locus_tag="PKV31_04950"
     CDS             complement(2843..4240)
                     /gene="lpdA"
                     /locus_tag="PKV31_04950"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB92239.1"
                     /translation="MKYDILVLGSGPGGYVAAIRASQLGFKTAVIERESLGGICLNWG
                     CIPTKALIKSAKVFEYLNHAGDYGITVDGGKADFGAIVKRSRGVAEGMSKGIQFLLKK
                     NKVDTIYGNGVLKKGKKIEVAAADGSKTTYEANHIIIATGGRAKELPGIAIDHKNIIG
                     YREALALESLPESMVVIGAGAIGVEFAYFYHTLGTKITVVEFMENGLVPREDAEISKE
                     LGKIYKKKGMNILGNTAVEKVDISGSKKKVTVKDRKTGATQVIECDIVLSATGVTPNV
                     ENIGLEEVGIQLDRGHIKVDSFYKTNVDGYYAIGDVIPTQALAHVASAEGITCIENIA
                     GMNPHSIDYNNIPGCTYCSPEVASVGYTEAAAKEAGYELKVGKFPFSASGKAAASGAK
                     EGFIKLIFDAKYGELLGAHFIGENVTEMIAEIVVARKLETTGHEMIKAIHPHPTMSEA
                     IMEAAAAAYGEVIHI"
     gene            complement(4886..5638)
                     /locus_tag="PKV31_04955"
     CDS             complement(4886..5638)
                     /locus_tag="PKV31_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92240.1"
                     /translation="MITYFTSFIYGWIGLAIFIFFILLFITAPYGRHTTKTWGPLIDN
                     RSAWIIMEIFVLIVINYFVFTGRLVQTPVNYIILGLFSLHYLNRSLIFPFRLRTSGKK
                     MPVIVMLMGMTFNLANGFIIGYFLGNIKTYELEWLTTPQFIAGIIIFFTGMIVNWRAD
                     GILIRLRAPGEIGYKIPKGDMFRFVSCPNLLGEIIEWGGFCILTWSLPGLAFFIWTFA
                     NLVPRAISHHKWYKQRFNDYPPERRAVIPFVI"
     gene            complement(5648..6625)
                     /locus_tag="PKV31_04960"
     CDS             complement(5648..6625)
                     /locus_tag="PKV31_04960"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB92241.1"
                     /translation="MKVAVTGASGHIGASLCRELLHHGYEVVALIHRDISALKGLPIS
                     MVQGDILNTESIKQLLGQCDMAIHAAAAIELAYKFDKNVYDTNVLGTINVLETAQEMG
                     VRRLVHFSSIHAFSQACYDQPLDETRAFVNDNSIFYDQTKRDSHRLALNACEAGLDVV
                     ILNPTSAIGPPDQKPSLLGKAVIDLYKGSLPFVVQGGFDFCDVRDIAFGAVKALEKGR
                     KGEAYLLSGHYHSIKELADFVMEAKNSKKRLVELPLYIANIAFPFVKFYSWLTKKPPL
                     FDKTYIDILQDGNKNVSSAKAEAELGFTSRDLRESVEDTVKWFKDNGRL"
     gene            7098..>8567
                     /locus_tag="PKV31_04965"
     CDS             7098..>8567
                     /locus_tag="PKV31_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNB92242.1"
                     /translation="MSYSKAKSVKELSVNGKSYHYSSLKDLDVNIDHLPFSIRILLEN
                     VLRNYDGFSITDEHIDTLLNWKPATEDKDIPFKPGRILMQDFTGVPAVVDIASLRAEF
                     VRHGKDGQKINPAIPVDLVIDHSVQVDYFGTDYAYDKNVEIEFERNKERYELLKWAQK
                     GMSNFTVVPPGMGICHQVNLEYLAKGVTARDGWLFPDTLVGTDSHTPMVNGIGVIAWG
                     VGGIEAEAAMLGQPIFFTLPEVVGLKLTGKIPDHCTATDMVLTITRLLRDAGVVGKFV
                     EVFGDGLNNLTVTDRATISNMSPEFGCTVTYFPVDNRTLEYMHSTNRSEEQIRIVEAY
                     CKENMLWREGEDKIKYSSIVELDLTTLEPTVSGPKRPQDKIFVKDLSGKFCEILKKEF
                     NRNYHPQSERAEYAMLIEGGSGTEFTRGKVPMNGESSYEVVADQDHRSVRIKQPNKEF
                     VLSDGSIVIAAITSCTNTSNPAVMVGAGLLARNAVERGLR"
BASE COUNT         2481 a         1676 c         1747 g         2663 t
ORIGIN      
        1 atgcctgtcg atggtaaatt ttctcttcct cttttagggt atgattacaa tgcattggag
       61 ccgaatattg atgctcagac catggagatt cattattcca ggcatcatca gggatatgtc
      121 aataacctta atgctgctat ttccggaaaa ccggagtctt cttattcctt ggaaaaaatt
      181 tgcgctatgg caagcaataa ggatatggca gtacggaaca acgccggcgg tcattacaac
      241 catagttttt tctggaaatt attgactcca ggtggatcta acaagcctac cggggatttg
      301 gcaagtgcca ttgaaaggga tttcgggtct tttgatgctt tccgggaaaa gttcaacgat
      361 gctgcaacaa aaagattcgg ttcaggttgg gcatggctgg tcaaaggtgc taataataaa
      421 ctgtatgtct attcttcgcc taatcaggat aatccactta tgacatcatt aggatataag
      481 ggaatcccga tcatcggaat cgatgtatgg gagcacgcat attatcttaa gtatcagaac
      541 aaacggggag actatattaa gaacttttgg aacatcgtca actgggatgt agctaataaa
      601 aattacagtg aaaaataatt taggatattt ggatttttaa aatattaata tacctttgcg
      661 aaccctttta cagaaaaggg taatattttg agtatttttt caggagagat ggcagagcgg
      721 tttaatgcgg cggtcttgaa aaccgttgac tgtaacaggt ccgggggttc gaatccctct
      781 ctctccgcag ggaaagcgtt gtaagttgat tcttgcaacg cttttttatt ttatggcata
      841 ggccagcagc actgcaataa ctaataacgc taagccaggt ccaggggctg gtgtggagaa
      901 aattattttt gcagggtcaa attccaagta agtagatgat taatagtctc aaattattgc
      961 tatgatggag tttttagctg acaagaaaaa taaaattgca gatatcaact aaatattgca
     1021 tacaaggtct ctgaaaacaa ggctagctat gatttagtcg ttattaaaca tctacctact
     1081 tcccgagaat ccacctcctt cctacgtatc caattgctgc cgattgaaaa attcaaagat
     1141 gcagatgttt aatagtcatc ataaatttat gccatctgct gactttcgat cttgattaat
     1201 tttttacgat tgcattcaga tagtttttat cctcaaggta ccattaagga tattctggag
     1261 ttgcatggga atttatgaaa atctataggt gaattcattg tcccaagacg caattccgtg
     1321 aatgttaacc agcttgcata cctcaagtac ctggcagctg aaaaaaaaga agttaaatca
     1381 aataacaatc ttcggcgtat gcttttatga tcttagtaac ctaatatctt cagcatagac
     1441 tctgaatcct gatcattggc ccatacccaa tctactaact cgccttcagg gttacggtct
     1501 atcaatacgt gctttggtga aggcagaagg caatgcttga tacctccata accactcaag
     1561 gcatcctgat aggctccgct gtgaaagaat cccagatata gtttttcctc tttacctact
     1621 gcaggaagat atacctgatt actgtgagct tcggaattgt aataatcgga attgtcgcaa
     1681 ctaagtccac cgatgttgac tcgtttgtac tcatggtccc agtgattgat gggtaacagg
     1741 atgaagtgtt cattgattcc ccaggcatcc ggtaaggtgt tcataaggga gttatcgacc
     1801 atataccagc attcgttgtc gttttgttgt ttctggccta tgactgagaa gaaatgtgct
     1861 ccgctctcac caacggtgta tttgccaaat tccgtatata tatcaggttc agggatgcca
     1921 ttttctgcac atgcatcctg aatctgacgc acaatctcca ggatcatgta tccgtaatcg
     1981 tattcaaaac ccaaactgtt cttgataggc agaccgcctc cgatattgat cgcttcaagg
     2041 gtggggcagg ccttcttcaa ttcgcaatat gcctttatac ccttgcgaag ctcgtcccaa
     2101 taatacacgt tgtctttgat gcctgtatct acgaagaaat gtagcatctt caggctgatg
     2161 tttggacgtt gggcgatttt ttcatggtag aaattaagaa tctctgattt tcggatgcca
     2221 agcctggagg tataaaattc aaaatgtggt tcttcctccg tcgcaacacg gattcctaca
     2281 ttaaactccc ggtctccgag gtattggctg aatttatcca attcttccca gttgtccata
     2341 acaggaagga cgtttgtgaa gtcggattga atcattccgc agattctacg gatgtatgca
     2401 tcattcttaa atccgttatt tacgatgatc ttgtccttat gcagcagccc atctgcgtat
     2461 agcttccata ttaagtcaat atcaaatgct gaagaggtct cgagatgtac atcattcttc
     2521 agtacttctt caagaacata gcggaagtgg cagcttttag tacaataggt ataattgtat
     2581 ttgccggtat actgcaattg aaaaatggca gtatcgaaaa agtttttggc tttcttgatc
     2641 tgatcagata ttttgggcag gtacacgagt ttaagtggtg taccatattt ttctatcagt
     2701 tcatatagct taattccatg gaaaaggagt ttattgtctt caagaccgaa tccctcctgt
     2761 ggaaaataaa aggtctgatt gaccaggtcg tggtaagtgt ggttcatccc cccagccctt
     2821 gtattctttt tttatgaata aattaaatat gtattacttc tccgtatgca gcagcagcgg
     2881 cctccatgat ggcttcgctc attgtcggat gtggatggat tgccttgatc atttcatgtc
     2941 ctgttgtttc caacttacgc gcgacgacga tttcggcaat catctcagtg acattttcac
     3001 ctatgaaatg ggcaccaagt aattcgccgt attttgcatc gaaaatcaac ttgatgaatc
     3061 cttctttcgc accggaggca gctgctttcc cggatgctga aaatggaaat ttgcccactt
     3121 ttaattcata accggcttct tttgcggcgg cttcggtata tccaacagat gccacttcag
     3181 gtgaacagta tgtacaaccc ggaatattgt tgtagtcaat tgagtgtgga ttcataccgg
     3241 caatgttttc tatacaagtg atgccctcag cgcttgctac atgtgccagg gcttgtgtcg
     3301 ggatgacatc acctattgca taataaccgt ccacattggt cttgtaaaag gaatccacct
     3361 tgatatgtcc tctgtccaat tggatgccga cttcttcaag acctatgttc tctacattgg
     3421 gcgttactcc tgtcgctgaa agcactatat cgcattcgat aacctgtgtg gcgcccgtct
     3481 ttcggtcctt gacggttacc ttctttttag aaccggaaat atcgactttt tcgacagctg
     3541 tgttacccag gatgttcatg ccttttttct tgtatatttt gccgagttcc ttcgatattt
     3601 ctgcatcttc ccgagggaca agaccgtttt ccataaattc tacaacagta atcttcgtgc
     3661 ccagcgtgtg ataaaaatag gcaaattcta caccgattgc acctgcgccg atgacaacca
     3721 tggattcggg gagggattca agagcaagtg cttcgcggta tccgatgata tttttatggt
     3781 ctatggctat acccggcaat tcttttgcac ggccaccggt tgcaatgatg atatgattgg
     3841 cctcatatgt tgtctttgaa ccgtctgcgg cagccacttc gattttttta ccttttttca
     3901 ggacgccatt gccgtatatt gtatcgactt tatttttttt caaaaggaat tgaatgcctt
     3961 ttgacatgcc ctctgcaacg ccacggcttc ttttgacgat agccccaaaa tctgcctttc
     4021 ctccatcaac tgtaataccg tagtcaccag cgtggttgag atattcgaat accttggcac
     4081 tttttatcaa cgctttggta gggatacatc cccaattgag gcagatgcca cctaaggatt
     4141 ccctttcaat gactgctgtt ttaaatccga gttgagaagc tctgattgct gctacataac
     4201 ctccgggacc acttcccaag accaaaatat cgtatttcat gctgaatttc gtttagtatt
     4261 ttagctaatt aagtcgtttt tcaaaaaatc gtgcaaatat atgtaattgt gaggacttcc
     4321 tcagggacat tttctaaatt gatactattg gaacaatacg ttaatccgtg aagtcaggac
     4381 ttaaataggt tcctttgcct gggttatacc cggatacatg caccgtaatg aggctataga
     4441 tttctttttc ttgtatcctt accggcaagt tgactggtat attttcaatt tatccataaa
     4501 tagatacccg ggaagcaatt acatttccat ccaacgactt gaatatttct ttgaaatatt
     4561 attgatatca ttggcttcct gttgcattga tgtggttgta ttaactgact gtcgggcata
     4621 attgagagct attagtttga atgaatggag tttggtaatt caattcatgt ttttcaatgt
     4681 taacaaaaca ggggcactaa taagaatttt ttacgagtcg tccgcgtcca atatactaaa
     4741 caaaaaatga aaatggaata atataaatgc aacaagtacg aaatcaaaga tgagttatcg
     4801 atcagctcct acaaataagg ggtatcgtgt cactattatt atcaatagca tattattcta
     4861 atgtctataa ctctgctctg tacaattaaa tgacaaatgg aatcacagcc cttctttccg
     4921 gtgggtaatc attaaatcgt tgcttgtacc atttgtgatg ggatatggct ctcggcacga
     4981 gattggcaaa agtccaaatg aaaaaagcaa gccccggaag actccaggtg agaatgcaaa
     5041 atcctcccca ttctataatt tcacctaaca aattagggca actaacaaat ctgaacatgt
     5101 cacctttagg aatcttatat ccaatttcgc ccggagctct cagacgaatt aaaatcccat
     5161 cggctctcca attgacgatc attcctgtaa agaatatgat gatccctgca ataaattgtg
     5221 gtgtagttaa ccattctaat tcataagtct tgatgtttcc aaggaaataa cctattataa
     5281 agccattggc aagattgaag gtcatgccca tgagcatgac tataacaggc atcttttttc
     5341 cggaagttct gagtctgaat ggaaatatga ggcttctatt gaggtagtgt aaggaaaaca
     5401 atcccagtat gatataattg acaggtgttt gaaccaatct gcctgtaaaa acaaaatagt
     5461 tgataactat taaaacgaaa atctccatga tgatccatgc tgaccgattg tcgatcaaag
     5521 gaccccaggt ttttgtcgtg tgtcttccgt aaggggctgt aataaataaa agtataaaaa
     5581 aaatgaagat tgccaaccct atccatccat aaataaatga agtaaaatat gttatcatag
     5641 aattagatta gagtcgacca ttatctttaa accatttgac cgtatcttca accgactctc
     5701 ttagatccct tgatgtgaag cccaattccg cttctgcttt tgctgagctc acatttttat
     5761 taccatcctg aaggatgtcg atataggttt tatcaaataa aggtggtttt tttgtaagcc
     5821 atgaataaaa cttaacgaaa ggaaaagcaa tattggcaat atataaaggt aattcaacca
     5881 atcttttttt tgagtttttt gcctccatga caaaatccgc caattccttg attgagtgat
     5941 aatgtccgct aagaagatac gcttcacctt tacggccctt ttccagggct ttcacagctc
     6001 cgaaagctat atcccgcaca tcacaaaagt caaatccacc ttgaaccacg aaaggcagag
     6061 atcctttgta caaatcaata actgctttgc ctaacaatga tggtttttgg tcaggtgggc
     6121 caattgcaga agttggattc aggatgacaa catcaagtcc ggcttcacag gcatttaaag
     6181 ccagcctatg gctatctcgt ttggtttgat cgtaaaaaat cgaattgtca ttaacaaatg
     6241 cccttgtttc atcgagtggc tgatcatagc aagcttgaga aaaggcgtga atagaactga
     6301 aatggaccaa ccgtctaact cccatctctt gggctgtttc tagaacattg attgtaccca
     6361 gtacatttgt gtcgtataca tttttgtcaa acttgtaggc taattcgatg gcagcagcgg
     6421 catgaattgc catatcacac tgtcctagaa gttgttttat tgattcagta ttgagaatat
     6481 cgccctgaac cattgaaatt ggaagtccct ttaaagcaga aatatctcta tgtatcaatg
     6541 caactacttc ataaccatga tgtaataatt cccgacagag ggaagcgccg atatggccgg
     6601 aagctcctgt tacagctact ttcatcagcc gaagttataa ttttttgaaa attttttgaa
     6661 attaatgatg aagaattatt aaattatata tatgtactat atttattttt ataaacataa
     6721 caaaaccaat acaaaatact ttaattatat atattatgta ttaaatatga actatattca
     6781 ttttgaataa aatggagaaa gggtaaagcg atgtagctaa tgaataattt tcatgtatta
     6841 gttattctga tcttgcatta ttttgttttt tgtcattcat taacagtata ataccttgaa
     6901 gttattatca ttaggtattg atttatggtt ttggggctgg taaattcttg ttattaacaa
     6961 cctttcttct gatttttcat tatgatataa cattatatcc attaagtgta attttgaaat
     7021 tttcggctta ccaactccgg gagtttttat agatttacgt ttttttagca attcattaaa
     7081 tttcaataaa tagagatatg agttattcga aagcaaaatc agttaaagag cttagtgtca
     7141 acggaaaatc ataccattat tcctctttga aggatttaga tgtcaatatc gaccatcttc
     7201 ctttcagtat aaggattctt ttggaaaatg tacttagaaa ctatgacggt ttcagtataa
     7261 ccgacgagca catagatact ctgctcaact ggaagccggc gactgaggac aaagatattc
     7321 cattcaaacc gggacgtatt ttgatgcaag actttacagg tgtacctgca gttgtggaca
     7381 tcgcttcact tagggctgag tttgtccggc atggtaaaga tggacagaag atcaatcctg
     7441 cgatcccggt tgacctggtt atcgatcatt cagttcaggt cgattatttc gggactgatt
     7501 atgcctatga caaaaacgtt gaaatagagt ttgaaagaaa taaggagcgt tatgagttgt
     7561 tgaagtgggc tcaaaagggt atgagcaatt ttaccgttgt tcctcccggt atgggcatct
     7621 gccaccaggt caatctggaa tatcttgcca aaggggtcac tgcacgtgac ggatggttgt
     7681 ttccggatac attggttggc actgactcac atacaccgat ggtcaacggt atcggtgtca
     7741 tagcctgggg tgttggagga attgaagcgg aggcagccat gttgggacaa ccaatctttt
     7801 ttactttgcc tgaagtagtt ggcctgaaac tgacagggaa aattcctgat cattgtacag
     7861 ctactgacat ggtgctcacg attacgagat tgcttcgtga cgccggagta gtagggaaat
     7921 ttgtagaagt attcggcgat ggacttaata acctgactgt gactgacagg gcaacaattt
     7981 ccaatatgtc tccggaattc ggatgtaccg taacctattt tcctgtcgac aacaggacac
     8041 tggaatatat gcattccacc aatcggtctg aagagcaaat caggatagtt gaagcctatt
     8101 gcaaggaaaa tatgttgtgg cgtgaaggag aagataagat taagtattct tcgattgtag
     8161 agcttgatct tacaacactc gagcctactg tttcgggtcc aaaaaggcct caggataaaa
     8221 tatttgttaa ggatctctca ggaaagtttt gtgaaattct aaaaaaggaa ttcaatagaa
     8281 attatcatcc acagagcgaa agagctgaat atgcaatgct gattgaaggg gggtcaggaa
     8341 ctgaatttac gagaggaaag gtaccgatga atggcgagtc cagctatgag gtggttgctg
     8401 atcaggatca tcgttcagtt aggatcaaac agcccaacaa ggaatttgtg ttaagtgacg
     8461 gaagcattgt cattgcagct attaccagtt gcacaaacac atcaaatcct gcagtgatgg
     8521 tcggagctgg tctccttgcc agaaatgcag ttgaaagagg ccttcgg
//
LOCUS       DAOQMC010000089         8402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3381_length_8402_cov_4.405056, whole genome shotgun sequence.
ACCESSION   DAOQMC010000089 DAOQMC010000000
VERSION     DAOQMC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3381_length_8402_cov_4.405056"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKV31_04970"
     CDS             complement(<1..821)
                     /locus_tag="PKV31_04970"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNB92243.1"
                     /translation="MTKSNKEQVGNKNDGVTPLMRQYNDIKSKYPDALLLFRVGDFYE
                     TFGDDAIKVAGILGIILTSRNNGGSDTELAGFPHHSLDIYLPRLVKAGYRVAICDQLE
                     KPSKEKKIVARGVTNVVTPGLTLDDSILQSRTNNYLCAIDIRQTDHFGIAFLDVSTGE
                     FMVAEGGEAVIESLFQRFKPAEVIFNKKDKKNIQQLIKSQFPVFPLDDWVFSHESSYN
                     KLTTHFQSNTLKGFGIEELHSAQIAAGAILQYLEMNENKEISHINRIARIPQESY"
     gene            943..1419
                     /locus_tag="PKV31_04975"
     CDS             943..1419
                     /locus_tag="PKV31_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92244.1"
                     /translation="MTKYFDSISSDEKKVLKDTFVYITLLIASAENKLTLAEKEAAIK
                     TIKVRGYEANSLFNEFYDEINNTFAEELEQASAEHKISRDEIDYFSRKISQVNEVLEK
                     LPSYVARQLYNDYKRFSHRIANADGGILGFAKVSIEERNVVDLPMIHQIEFDDEEE"
     gene            1519..2364
                     /gene="prmC"
                     /locus_tag="PKV31_04980"
     CDS             1519..2364
                     /gene="prmC"
                     /locus_tag="PKV31_04980"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNB92245.1"
                     /translation="MNTPSRDLPSLVSALTGHYGNRESENILRILYEDYLILPRKTAL
                     EDIPPSKVIKLKAALDLLLKDYPIQYITGKVWFYGYLFLVGPGVLIPRPETEELVELA
                     LKNINKSDSCRVIDIGCGSGIIPIVIKRQRPGVMVDAVDISQQAIEFTRKNAELLQVE
                     LNIFEANILHPEWTFFEDYDFIISNPPYILEDEIPEMSVSTVLYEPHIALFSGEAMTF
                     YKAILFFGQKKLKIGGKILLECSEFHAEEVRNLALSMGYSEAYLHKDLQGKDRMIEVV
                     NSNSF"
     gene            2673..4058
                     /locus_tag="PKV31_04985"
     CDS             2673..4058
                     /locus_tag="PKV31_04985"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNB92246.1"
                     /translation="MSNNEYIAANRDRFLNELVEWLKIPSISSDSRAKEDMGRAAEYL
                     KARLVEQGVDRAEIYPTAGHPIVYAEKITDPAKPTVLVYGHYDVQPPDPLDLWDSPPF
                     EPVIKITDEHPEGAIFARGSCDDKGQVYMHVKAFEVMAKNNDFPCNIKFMIEGEEEIG
                     SVHLGKFCEDHKDMLKSDVILISDTSIIDNQTPSITTGLRGLAYMEVEVTGANRDLHS
                     GVYGGAVANPINVLAKMIAGMHDENNHITIPGFYDAVENLSREERDAMAEAPFSLEGY
                     QRDLGIDSIYGENGYTTLERASIRPTLDVNGIWGGYIGEGAKTVLPSKAFAKISMRLV
                     PHQNSTVISKLFSDYFTSIAPKGVKVKVTPHHGGEPVVTPVDSLEYKAAAMAMKDTFG
                     TEPIPQRSGGSIPIVALFESVLGTKSVLMGFGLDSDDIHSPNEHYGVFNFFKGIETIL
                     RFYPNYAALKQ"
     gene            4099..4830
                     /locus_tag="PKV31_04990"
     CDS             4099..4830
                     /locus_tag="PKV31_04990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014701238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB92247.1"
                     /translation="MIKNLKFLFLMTLLLSLLSNDASAQGGKAKTKSEKTEKTDSKTD
                     SKTTKMDSLSYGLGVLLGQNIKQLDIENIDAKVIAQAIQDVLDNNQLKVTPEQANMLV
                     SNEMAAAAERKAGPAIEEGRKFLAENAKKPGIKTTASGLQYEVIQSGTGATPKLTDKV
                     TTHYRGTLINGNQFDSSYDRGEPATFPVNGVIKGWTEALQLMKEGDKWRLYIPYNLAY
                     GERGAGRDIPPYATLIFDIELIKVN"
     gene            4928..6439
                     /gene="cysS"
                     /locus_tag="PKV31_04995"
     CDS             4928..6439
                     /gene="cysS"
                     /locus_tag="PKV31_04995"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNB92248.1"
                     /translation="MSIRPLYIFNTLSGEKELFKPILDDRVGMYVCGPTVYNDVHLGN
                     CRTFVSFDVVFRYLLHLGYKVRYVRNITDVGHLVGDQDTGSEDKIGQRARLEQLEPME
                     IVQKYSNGFHHVMKLLNALDPSIEPSATGHIIEQMEMVQQILDNGLAYEVNGSVYFDT
                     VKYASQSDSYGKLSGRVIEELFSETRDDLKNQDEKRHPSDFALWIKADDTHLMRWQSK
                     WSVGFPGWHLECSAMSTKYLGKTFDIHGGGQDLKFPHHENEIAQNHGACGCQPAHYWM
                     HGNMLLLNGRKMSKSEGNSILPMELIAGSNPIIPESFSPMAIRFFMLQSHYRSTLDLT
                     HDALKAAEKGLSRLRDAYGLLAKLQVNPGREDLKVNQTIQSEIDGMYEDMSNDFNTSK
                     TIARLFELASIINIYYNNPQTDAGLNEESIELLQKAFDHFLGSILGIRLKDDVVGDSE
                     NFTDGLMEVILELRAKARENKDWPTSDLIRDKLSRLDIQVKDGKDGVTWSIVK"
     gene            6467..7090
                     /locus_tag="PKV31_05000"
     CDS             6467..7090
                     /locus_tag="PKV31_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNB92249.1"
                     /translation="MNEAIIAGAISGMILGTLPGGVVFAVLQQSIEQGWRKGVGIATG
                     VVASDFMLILFVNLSTHGLAFVNKYDAYISMAGVALLLILGIGNLFIKSSLPISSRST
                     KGGAFYLFSTGFLLNTLNPVNFFMWMTITTQIQTRDFFNFTNMIWYFGTTLTMIFTCL
                     TMISFFSNRLRKWMTPEHLRYLNIVIGIIFIGLAIKLGYDAYMKHFF"
     gene            7285..7890
                     /locus_tag="PKV31_05005"
     CDS             7285..7890
                     /locus_tag="PKV31_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNB92250.1"
                     /translation="MAGNNPPTLKFALLKYAGGGDWYNDVNSLHNLAQFCNQKMGTNF
                     DLDYATVEPSSAELFAYPITYMTGHGNVVFSDADAQNLRKYLIGGGFLIINDDYGMDP
                     FIRPALKKVFPELSLMEIPLNHPVYTSLFEFKGGLPKIHEHDGKPAQAYGLFWEGRLV
                     ALYIFETDLGDGWDDVHNDPFELRLKALQMGANIVKYAFER"
     gene            7902..8279
                     /locus_tag="PKV31_05010"
     CDS             7902..8279
                     /locus_tag="PKV31_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNB92251.1"
                     /translation="MDIDIFREYCLSKPFVTEECPFGPDTLVYKVLGKIFALTGLDEV
                     ELSLNLKCDPEYAIELRERYASIIPGYHMNKTHWNTVHTGALNNDKLLKELIDHSYLE
                     VVKKLPKKLQAAFKNPGHNDENK"
BASE COUNT         2470 a         1624 c         1856 g         2452 t
ORIGIN      
        1 atataggatt cctgaggaat tcttgctatt ctgttgatgt ggctgatttc cttgttctca
       61 ttcatttcaa gatactggag gatggcacct gccgctatct gggctgaatg taattcttca
      121 atgccgaatc cttttagtgt gttgctttga aagtgagtcg tcaacttgtt gtatgaagat
      181 tcatgactga aaacccaatc gtctaacggg aaaacaggaa attgagattt gatcagttgt
      241 tgaatattct tcttgtcttt cttgttgaaa ataacctcag caggtttgaa tctttgaaaa
      301 aggctttcta tcactgcttc acccccttct gctaccatga attcgccggt tgatacatcc
      361 agaaaagcga tcccaaaatg atcagtctgg cggatatcaa tggcgcataa gtaattattg
      421 gtacgggatt gaagaatgga atcgtctaac gtcaatccgg gtgtgacaac attggttacc
      481 ccacgagcga caatcttctt ttccttactg ggtttttcaa gctgatcaca gatggcgacc
      541 cgatagcctg ccttgacaag cctggggaga tatatatcca gcgaatgatg tggaaatcct
      601 gccaactcag tatctgaacc gccattattg cggcttgtca agatgatgcc aagaatgccg
      661 gcaactttta tggcatcatc tccgaaagtc tcataaaaat cacccacacg aaacaacagc
      721 aatgcgtccg gatatttgga cttgatgtcg ttatattgcc gcattaatgg tgtcacacca
      781 tcattcttat ttccgacttg ctctttgttt gattttgtca aaatttttat tttaaacggt
      841 taaaattaga aacaacacta tatatttgcg aaaaatttat ataaatttat tcattcgaat
      901 aagtgatttt tcattaattc atttagtcat aaatagttaa acatgacgaa atattttgat
      961 tcgatttcct ctgatgaaaa gaaagtttta aaggacactt tcgtttacat taccctgctt
     1021 attgcctctg cagaaaataa gttgacgctt gctgaaaagg aagctgctat caagactatt
     1081 aaggtgagag gttatgaagc aaatagctta ttcaatgagt tttatgatga gatcaataat
     1141 acatttgcag aagaattaga gcaggcaagc gcggaacata agataagcag agatgaaatt
     1201 gattattttt cccgtaagat cagtcaggtc aatgaagtac ttgaaaaatt gccttcttat
     1261 gttgcaagac aactttacaa tgactacaaa cggttttctc accgtatagc aaacgctgat
     1321 ggcggaattc ttggatttgc caaggtgtcc atcgaagaga gaaatgtagt ggatctgcct
     1381 atgatccatc agatcgaatt tgacgacgaa gaagagtaac tgagattgac aggattcatc
     1441 ctttgtcagg acttgtattt cggtggagat gcctggccgg acaatattct ttgagtaatt
     1501 gccttacgaa gcaaaagaat gaacactcct tcccgtgact taccttcact tgtatcggct
     1561 ctgaccgggc attatggcaa tcgggaatca gaaaatatcc ttcggattct ctatgaggat
     1621 tacttgattc tgccacgtaa aacggcatta gaggatattc ccccatccaa agtaattaag
     1681 ttgaaagcag cattggactt gttgttgaag gattacccaa tccaatatat taccggaaaa
     1741 gtctggtttt atggctacct ttttctagtg ggccccggtg tattgattcc aagaccggaa
     1801 accgaagagt tggtcgaatt agccctcaaa aacatcaata agtctgattc ctgccgggtt
     1861 atcgatatcg gttgtggtag cggcatcatt ccgatagtca tcaagagaca aaggccgggt
     1921 gtcatggttg atgctgttga tatatctcaa caagcgattg aatttacccg aaaaaatgct
     1981 gaattacttc aagtggagct caatattttc gaagcaaata ttcttcatcc tgagtggaca
     2041 tttttcgaag attacgactt cattattagc aatccaccct atatactgga ggacgagatc
     2101 cctgagatgt ccgtatctac tgtcttgtat gagcctcata tagccctatt ctcaggggag
     2161 gcaatgactt tctataaggc aattctgttt tttggacaaa aaaagcttaa aatcggcgga
     2221 aagatactgc ttgagtgcag cgaatttcat gcagaagagg ttaggaatct tgccctgagc
     2281 atggggtatt ccgaagccta tttgcataaa gatctgcagg gaaaggacag gatgatcgaa
     2341 gtggtgaatt caaacagttt ttgaacacga aatttccttt caccttaaaa ttggaaatat
     2401 tcaattattg acctcaatga gagtaaggat aaaattatga tcaacaattt attaaataaa
     2461 atatattaat tatatttata aatttaaata ttttagtaaa ataaacatgt aataaagata
     2521 ttatattgtc ggactatatt tatctccctt tttgcaataa ttattcatcc ctgaaaattt
     2581 ccatatcttt tttccgttat aactttttaa tagccacaaa acattagcta ttttttgtac
     2641 ttttgcactc tttattataa aaatttaata atatgagtaa taatgaatat atagcagcca
     2701 atcgcgaccg ttttctcaat gaattggtcg aatggttgaa aattccgtcc ataagctcag
     2761 attcaagagc aaaagaagat atgggacgtg ctgccgaata cttgaaagca agattggttg
     2821 agcagggagt ggaccgggca gagatctatc caactgccgg acatcccatt gtatatgctg
     2881 aaaaaattac cgatccggct aaaccaacgg ttctggtata cggtcattat gatgttcagc
     2941 caccggatcc gttggatcta tgggattcac caccatttga gccggttata aagattacgg
     3001 atgagcatcc ggaaggcgct atctttgcac ggggatcttg cgatgataaa ggacaggtgt
     3061 atatgcatgt caaggctttc gaggtaatgg caaagaacaa cgacttcccg tgtaacatca
     3121 aatttatgat tgaaggagag gaggagatcg gctccgtaca tctcggcaaa ttttgtgaag
     3181 atcataaaga catgttaaaa tctgatgtta tcctgatttc tgacacgagt attattgata
     3241 atcagacgcc ttcaatcacg acaggtttac gaggccttgc atatatggag gttgaagtga
     3301 caggagccaa cagggatttg cactccggag tatatggcgg ggcggtagcc aatccaatta
     3361 atgttctggc caaaatgatc gccgggatgc acgatgaaaa taaccatatc actatacccg
     3421 gattctatga tgcggtcgag aatctgtccc gcgaggaacg agatgcaatg gcggaagcac
     3481 ctttttcttt ggagggctac cagcgtgacc tcggtattga ctcgatatat ggtgagaacg
     3541 gatatacaac tttagaaagg gcgtctatta ggccgactct cgatgtcaat ggcatctggg
     3601 gtggatatat cggtgagggc gccaagacgg tactgccgtc caaggcattc gccaagatca
     3661 gcatgaggct ggttcctcat cagaattcta cggtaatcag taaattattt agtgattact
     3721 ttacctccat agccccaaaa ggcgtgaagg tcaaggttac tccacaccat gggggagaac
     3781 cggttgttac accggtggac tcactggagt ataaggctgc cgcgatggcc atgaaggata
     3841 cttttggtac ggaacctatt cctcagcgta gtggtggaag tattccgata gttgcccttt
     3901 tcgagtcggt attaggtact aaatcggtat tgatgggctt tggactggat tcagacgata
     3961 tccattcgcc caatgaacat tacggagtat tcaatttctt caagggtatt gagacaatcc
     4021 tgcggtttta tccaaactat gcagcattga agcaataaat tcattaataa ttttactaat
     4081 caaacaattt tcagaaagat gattaaaaat ctgaaattcc tttttttgat gaccttattg
     4141 ctctcattgt tgagcaatga tgcctcagca caaggaggaa aagctaaaac caagtctgaa
     4201 aagactgaaa aaactgattc taaaactgat tctaaaacaa ctaaaatgga ttcattaagt
     4261 tatggactgg gcgttctgct tggtcagaac atcaagcagt tggacattga gaatatcgat
     4321 gccaaagtaa ttgcccaggc cattcaggat gtattggata acaaccagct aaaagtaact
     4381 cctgaacaag ccaatatgct ggtaagtaat gaaatggcag ccgcggctga gcgtaaagcc
     4441 ggtccggcta ttgaagaagg gaggaagttc ttggccgaga acgcaaagaa acccggcata
     4501 aagactactg caagtggttt gcagtatgaa gtaattcaat ccggtacggg tgcaacacct
     4561 aagttgactg ataaagtgac aacacactac cgaggtacat taatcaatgg caatcaattc
     4621 gacagttcct acgacagggg tgagccggct acctttccgg ttaatggcgt aatcaagggc
     4681 tggactgaag cactgcaatt gatgaaggaa ggtgacaaat ggagacttta cattccttat
     4741 aaccttgctt atggggaaag aggtgccgga cgtgacattc caccatatgc tacattgatt
     4801 ttcgatattg aattgattaa ggtcaattaa tgaccttttt tagatagata ccggagtatc
     4861 tcactggata ttccggtatc atttttttaa cccgtagtta agcctggtat tttaaattcc
     4921 tgattctatg tcaatacgcc cattatatat ctttaataca ctttccggag agaaggaatt
     4981 gttcaagccc atcctcgatg acagggtcgg tatgtatgtg tgtggtccaa ccgtgtacaa
     5041 tgatgtgcac ctgggaaatt gcaggacctt cgtgagcttt gatgtcgtgt tccgatacct
     5101 tctgcacctc gggtataaag ttcgctatgt gcgaaacatt accgatgtcg gtcatttggt
     5161 gggtgatcaa gatacgggtt ctgaagataa aatcggccaa agagcacggt tggaacaact
     5221 ggaaccaatg gagatcgttc agaaatattc caatggtttt catcatgtta tgaaattgct
     5281 gaatgccttg gatccaagta tagagccctc tgcaaccggt cacatcatcg agcaaatgga
     5341 aatggttcaa cagattctgg ataacggcct ggcttacgaa gtcaatggtt ccgtctattt
     5401 cgataccgta aaatacgcat cacagtctga ttcctatggt aaattatccg gaagagtaat
     5461 tgaggaatta ttttcggaaa cacgtgatga tcttaagaat caggatgaaa agcgtcatcc
     5521 cagcgatttt gcattgtgga tcaaagcgga tgatactcac ctcatgcgct ggcagtcaaa
     5581 gtggtctgtg ggtttccccg gttggcatct tgagtgctca gccatgagta caaagtattt
     5641 aggcaagacc tttgatattc atggaggagg gcaagatctt aagtttcctc atcatgagaa
     5701 tgaaatagcc caaaatcacg gtgcatgcgg ttgccagccg gcgcattatt ggatgcacgg
     5761 aaatatgctt ctgctcaatg ggcgtaaaat gtcaaaaagc gaaggaaatt ccatcctgcc
     5821 aatggaactg atagcaggta gtaatccaat tatacctgag tcattttctc cgatggcaat
     5881 tcgttttttc atgctgcaaa gtcattatcg cagtacactg gacttgaccc acgatgccct
     5941 gaaggctgca gaaaagggcc tgtcaagact tagagacgcc tatggtctgc tggctaagct
     6001 ccaggttaat cccggaaggg aggacctgaa ggtcaatcaa accatccaga gcgaaataga
     6061 tggtatgtat gaggatatga gcaatgactt caatacatca aaaaccattg caaggctgtt
     6121 tgaacttgct tccatcatca atatttatta taataatcca cagactgatg ccggtcttaa
     6181 tgaggagtcc atcgaattgc tccaaaaggc attcgaccat tttttgggta gtatcctcgg
     6241 aatccgcctt aaggatgatg tagtaggaga cagtgaaaat tttacggatg ggttgatgga
     6301 agttattctt gaactgaggg ccaaagccag agaaaataag gattggccta catctgacct
     6361 tattcgcgat aaattgtcaa ggttggacat tcaggttaag gacggcaagg atggtgttac
     6421 ctggagtatt gtcaagtgat ctttcagcag ataaagaacc cgggaattga atgaagccat
     6481 cattgcaggt gccatttctg gcatgatatt ggggaccttg cccggaggcg tcgtatttgc
     6541 cgtgctccag caaagcattg aacaaggttg gcgtaaaggc gtcggaatcg ccacaggggt
     6601 agtagccagt gacttcatgc tcattctctt tgtcaacctg agcacacatg gactggcttt
     6661 tgtcaacaaa tacgatgctt acatcagcat ggccggagtt gcactgttgc tgatactcgg
     6721 tatagggaac cttttcatca aaagcagcct gcctatctca tctcgttcaa ccaagggcgg
     6781 agcattctat cttttttcaa caggcttcct gcttaataca cttaatccgg ttaatttctt
     6841 catgtggatg accatcacaa cacagatcca gacacgtgat ttcttcaatt tcacgaatat
     6901 gatttggtac tttgggacta ccttgaccat gatatttacc tgtcttacca tgatcagctt
     6961 tttttccaac agattgcgta aatggatgac acctgagcat ttgcgttatc tgaatattgt
     7021 catcgggatt atttttatcg ggttggcgat aaaattgggc tatgacgcat atatgaaaca
     7081 cttcttttag tttccggtat tcactattga attcttcttt gaataatata cttgtaatgc
     7141 tgattgccgc tttgccattc aaaaccatta tctttgtccg gtcgtaaata cgatttttat
     7201 aacacaattc cttttacggc gttatattac catatgaaga gttttattag ctatttattt
     7261 ttattaacat tgccgtttca atcgatggca ggcaacaatc ctccaacact gaaattcgca
     7321 ttgctgaaat atgccggcgg aggtgactgg tacaatgatg tcaacagcct gcataatctg
     7381 gctcaattct gcaaccagaa aatgggtaca aattttgatc ttgattatgc taccgtagag
     7441 ccttcaagtg ctgaattatt tgcttatccc attacttata tgacaggcca tggtaatgtg
     7501 gtcttttctg atgccgatgc tcaaaatttg cggaaatatc tgataggagg aggtttcttg
     7561 atcatcaacg atgattacgg catggacccc ttcattcggc ctgcactgaa gaaggtcttt
     7621 ccggaattgt cactcatgga gatcccgctt aatcatcctg tctatacttc actttttgaa
     7681 ttcaaaggag gattgccgaa gatacacgaa catgatggta aacctgctca ggcttacggg
     7741 cttttctggg aaggaagact tgtggccctt tatatttttg agacggatct aggcgatgga
     7801 tgggatgatg tacacaatga cccgtttgaa ctaagactca aagctttgca gatgggagcc
     7861 aatattgtca aatacgcttt tgagcgttaa ttacctgttc aatggacatt gatatattca
     7921 gggaatattg tctaagtaaa ccatttgtga cagaagaatg tccatttggt ccggatacac
     7981 tggtatacaa agttttgggt aaaattttcg cacttacagg gcttgatgag gttgaactaa
     8041 gtcttaacct gaagtgtgac cctgaatatg ctattgaatt gagagagcga tatgcttcca
     8101 ttattccggg gtaccatatg aataagacac attggaatac tgttcataca ggagcattaa
     8161 acaatgataa gctactgaag gaattgatcg atcactctta ccttgaggta gtgaaaaaat
     8221 tgcctaaaaa gctgcaggca gcatttaaaa atcctggaca caatgacgaa aataaataag
     8281 ttgctgattg tcgggcaagg attggccggt actgtgtttc attttacttt gtttcagcga
     8341 ggaatccatg ctgatatcat cgattgcgat tcgccgggaa aagcatcatc agcatctgca
     8401 gg
//
LOCUS       DAOQMC010000090         8375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3403_length_8375_cov_4.924760, whole genome shotgun sequence.
ACCESSION   DAOQMC010000090 DAOQMC010000000
VERSION     DAOQMC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_8375_cov_4.924760"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..679
                     /locus_tag="PKV31_05015"
     CDS             89..679
                     /locus_tag="PKV31_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB92252.1"
                     /translation="MCHSGSAVFFMKNKGRYQSLEEIIKACAENDRIGQEQLYRLYFT
                     SMVVMCRNYMTNEEDILSVVNDGFLKVFVNLTKSPVLTNVEGWIRRIIYNTMIDFHRS
                     LKSYKANISLVDTIFDSTVSQSPDEDTDYIFRLIDKLPDVTKTVFTKYAIEGYSHREI
                     SEYLNIRENTSKWHLFEARKKLREWLGVHYLNNQAG"
     gene            640..1908
                     /locus_tag="PKV31_05020"
     CDS             640..1908
                     /locus_tag="PKV31_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92253.1"
                     /translation="MAWSSLFKQSGRMKGLEERGWEDMLQRLNSTLPTHTKTGGSFRY
                     VAVFVMILIFFSTSSYLTFKHFMLDDTSDTILPGSAAPERLAGHVDLYSSTNGIDLAV
                     KTADESNNKDGIAVSKFAIEANYGVNNASKSARDVSNRINKSLKAHPDYTESISNVSF
                     DGSMENGFTQNTHKEENAGIRTHQEGITKRSSGIEDIEKLLMAETELLSSVEQNSPVI
                     ALKSRSTDRRLAFSSILGAYSDIHGRYFGMDIAPQVDFKISKGTSVFFRLPIRFKHTL
                     DNEIVRDVSNELNHNFEQKNLFLPDNLDIQSGKVNANTMDVNMVLGVSQRLGNKVALS
                     TYGLCGYENVLGLIQAFSSIPEFSSAANIAYRTANTSIAAKRDGWVYGGGVEAEIRLG
                     SRHSLIGGMNYYFNSDKSFEAGLGYRFRIY"
     gene            complement(2362..4314)
                     /gene="dnaG"
                     /locus_tag="PKV31_05025"
     CDS             complement(2362..4314)
                     /gene="dnaG"
                     /locus_tag="PKV31_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNB92254.1"
                     /translation="MIPSKTVEEIFQKARIEEVVGDFLVLKRRGVNMIGHCPFHNEKT
                     PSFVVSPGKNIFKCFGCGRSGTPVGFIMEHENMAYGDALRYLAKKYNVTVEEEARTPE
                     QNEVARQRESLLLINEFAMKHYISNLTETEYGRNIGLSYFRERGFHDVTIEKFGLGFA
                     FPDGHNLINEAKIKAFNIDLMKQIGLVTQNDRDFFVNRVMFPIFNLSGRIIGFGGRSL
                     TISEKAPKYLNSIDSDIYNKSKILYGFFQGKNAIRKEDNCYLVEGYTDVISLHQAGIE
                     IAVASSGTALTGEQAQLIKRFTDNVTLLYDGDAAGIKAAQRGLDILLQQNLNVKIAVL
                     PDGHDPDAYLKSHGAQALKDFVTRESRDFILFKIAGVAEEAKNDPIKKVTLAQDILQS
                     IARIPDNLKREVYVNQTAKILDIREEVLQSELNKAVRKFSDQQRLEAIREQRLDNSQQ
                     KEYTNKPSARQDASITPPTDNMPLHSILKVLIIYGNKTFEYDGEELVKDRIFKDIAEE
                     MDYIDHQLYLELLNEYQNREKGGENNSPEYYMNHPRQEIRDMVLRWVDPGYEFSQNWI
                     DKYQHPLQYQLMPDENYRNEYLQAFDLLKMNNLNKLAEDNKNKIKEFIENGDQEGLQV
                     QLQAQILINKERNKLANKLGMVIIPK"
     gene            complement(4451..6643)
                     /locus_tag="PKV31_05030"
     CDS             complement(4451..6643)
                     /locus_tag="PKV31_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNB92255.1"
                     /translation="MSLKRSNAIDIIHNRFDIDFPVNTPDKVKISGFYGENVFHDEAM
                     KKYLSESAYFSVKAAIQSGTNLTKEVANSVAEAMKNWAMDKGATHYAHWFQPLTGKTA
                     EKHDSFFTITADGKAIEEFGGEQLVQQEPDGSSFPGGGLRSTFEARGYTSWDPSSPAF
                     IIEVGNGKTLCIPTIFVTYSGESLDYKLPLLKSVAYLENAALEVCKIFDESIERTHAT
                     LGWEQEYFLVDESFLIARPDLMITGRTLLGRQSHKGQQLEDHYFGSIPERVLNYMRDL
                     ETECHKLGIPVRTRHNEVGPGQYELAPMYEEVNIAIDHNQLLMDIMERVAKRHKLAVL
                     LHEKPFNGVNGSGKHNNWSIATNTGKNLLSPGKNPGKDLKFLTFFVNTIRAVKEHADL
                     LRASVASASNDFRLGANEAPPAIISVYVGKALTEVLHFIAEGKESDKTMVKEVLSLLK
                     KSPNLELDNTDRNRTSPFAFTGNKFEIRMVGSSMNCSAPMTIMNTIVGNQLTEFKKEL
                     DLLTSKGESMDTAILSILRKYINESLPILFEGDGYSEEWQKEAEKRGLSNTPNTPDAL
                     KVYKMDKTSELFQKTGIFSEIELEAHYTVMVENYNLKLEIEARVLSDLCKSYVLPACY
                     KYQTGIAENIYTLKQLGLQEDAFRPQSNLLNEITHHINNVAEKVETLNQSVNKAVSEA
                     EPEEEAELFCHLVKPIMAEIRESVDHLEEIVEDGLWPLAKYREMMFIR"
     gene            6882..>8375
                     /locus_tag="PKV31_05035"
     CDS             6882..>8375
                     /locus_tag="PKV31_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92256.1"
                     /translation="MLILFCLPLEAQVDGSTVDIEVIDEVIESILASNPDLTEAQIND
                     LTDRLSVLAQSKVDLNESDLKVFEELLLLNELQIREILLHREVYGDFIALYELQTVPS
                     LTLSDIRRIWPFLKGIGTPGISPFSWSMLYKGSKTIISRYSRVLEQASGYVGDSSKYL
                     GSRDQISFRMRHVVPGRVSIGIGVEKDAGEPLNTNYNPFLFDFVTFHAYLENVAKQIK
                     QLALGDFQVSAGQGLIMFSGYGFGRNAFTTAVKRNERAIRPSTSLNEINALRGAAVGI
                     ELAPGWQMIAFASLKKSDGTLIIPDSLSGDSDVLISSLQMSGLHRTESEIRNGKHISM
                     FTTGGSLKYNGNRFQFGANAVYDQLDKRVEPADRVYNRFYFKGKNALNASVDYTYYFR
                     NLIFYGETAINPENAVATVNGLLVGLHPKVNLALLFRHLPKEYYSLHGKVFSDQSQTT
                     NETGFYAGLEVKPSPRFLVNIYADFFRHGWPTFSANGPSEGSAYLFKA"
BASE COUNT         2321 a         1640 c         1731 g         2683 t
ORIGIN      
        1 aaattcaaaa taaaattcca acgaatcaaa tgttaaattc gtctttagag tgaaacaaat
       61 attgaaagat aggtaaaagc atgtaaattt gtgtcattcg ggttctgccg ttttttttat
      121 gaagaataaa ggaagatacc aatctcttga agaaataatc aaggcatgtg ctgagaatga
      181 ccgaattggc caggagcagc tatatcgcct ttatttcact tcgatggtgg ttatgtgcag
      241 aaattatatg accaatgagg aagatatcct gagtgttgtc aatgacggct ttctgaaggt
      301 attcgtcaat ctgaccaaat ctccggttct taccaatgtg gaaggatgga ttaggaggat
      361 tatatataat accatgatag attttcatcg gagtcttaaa tcatataagg cgaatatttc
      421 tttggtggat acaatctttg acagcactgt gagccagagt cccgatgaag atactgacta
      481 tattttcaga ctaatcgaca aattgccgga tgtgaccaaa actgttttta ccaaatatgc
      541 gatagaaggt tattcacata gagaaatttc ggagtatttg aatattcggg aaaatacgag
      601 taaatggcat ctttttgaag ctaggaaaaa gcttcgggaa tggcttggag ttcactattt
      661 aaacaatcag gcaggatgaa aggattggaa gaaaggggat gggaagatat gctgcaaagg
      721 cttaattcga ctttgccgac acacacaaag accggaggct cctttaggta tgttgctgtt
      781 ttcgtaatga ttttgatatt tttttcaaca agtagttacc tgacttttaa gcatttcatg
      841 cttgatgata cctcggatac tattctaccc ggttctgctg ctcctgagag attagctggg
      901 catgttgatc tttattcttc aaccaatggt atcgatttgg ctgtcaaaac tgctgatgag
      961 agtaataaca aagatgggat tgctgtaagc aaatttgcca ttgaagcaaa ttatggtgta
     1021 aataatgctt caaaatcagc gcgtgatgtc tccaatagaa taaacaagag tctaaaagca
     1081 caccccgatt acacagaatc catttcgaat gtttcttttg atgggtcaat ggagaatggt
     1141 tttactcaaa atacccacaa ggaagagaac gccgggataa gaactcacca agagggcata
     1201 acaaaaaggt cttctggtat tgaggatatt gagaagttgc taatggcgga aactgaattg
     1261 ttgtcttcag tagaacagaa ttctccggta atagctttga aatcaaggtc aactgaccga
     1321 aggttagctt tttcttctat acttggagca tattcagata ttcatggaag gtattttggg
     1381 atggatattg cgccccaggt tgattttaaa ataagcaaag gtacatctgt cttctttagg
     1441 ttgcccataa ggtttaaaca tacacttgat aatgaaattg tgcgggatgt aagtaatgag
     1501 ctgaaccata attttgagca gaaaaatctt ttcttgccgg ataatttgga tatacaatca
     1561 ggtaaagtaa atgccaacac catggatgtc aatatggtat tgggggtaag ccagagactg
     1621 ggaaacaaag tagccttaag tacctatgga ctttgtggct atgagaacgt cctcggcctt
     1681 atccaggcct tttcaagtat tcctgaattc tcctcagctg caaacatagc ctatcgaact
     1741 gcgaatacgt ctattgcagc aaaaagagat ggctgggtct atgggggagg agtcgaagct
     1801 gaaatcaggt taggaagccg acacagcctg attggtggta tgaactatta ttttaattca
     1861 gataaaagct ttgaggccgg gttaggctat cgatttagaa tttattgata atcagcctag
     1921 tagaaggatt gggtttgcga agggaggagc cgcatataat gaggaggagt ttcctgttga
     1981 attaggtagt tgagtaatac cgatggactt attattgctc agtactatct gtttcgactt
     2041 ttgataatag gtcttgaatt atgtaatttg tattacagat tatctcacta atagccgggc
     2101 gccaagaatg gcaggtatta gccaactacc tacttcgatt tttccatatt ctacctaccc
     2161 atttccagat caattatacc caacccttcc gatacaaatg gcgtaaagga tattatgatg
     2221 tgaaagttat accaggatga cttatataat agtccaataa atttgaggta tacttgtccg
     2281 gatagttatg cgactagagc tctactctgt cccgggagtg aaacaatccg gggttccggg
     2341 atgccgaagg tacattcagt attacttcgg aataatcacc attccaagtt tattggccag
     2401 cttgtttcgt tccttattga tgaggatttg tgcctgcagt tggacttgaa gaccttcttg
     2461 gtcgccattt tcaataaatt ccttgatctt attcttattg tcttctgcaa gtttgttcag
     2521 attgttcatc ttaagaaggt caaatgcttg taaatattca ttccggtaat tttcatccgg
     2581 catcaattgg tattggagtg gatgttgata tttatctatc cagttctgac tgaactcata
     2641 tccgggatca acccacctaa gcaccatatc tctgatctcc tgcctgggat ggttcatata
     2701 atattccggt gagttattct ctccgccttt ttccctattt tgatattcat tcaataattc
     2761 aagatataac tgatgatcaa tataatccat ttcttcagca atatccttga agattctgtc
     2821 cttgaccagt tcttcgccat catactcaaa ggttttgttg ccgtagataa tcaatacctt
     2881 caatatggaa tgcaatggca tattgtctgt gggaggggtt atacttgcat cctgtcgggc
     2941 tgatggctta ttggtatatt ctttttgttg gctgttgtcc aggcgttgct ctctgatcgc
     3001 ctcaaggcgt tgttgatcgg agaatttacg tacggccttg ttaagttctg attgcaggac
     3061 ttcctcccgt atatcgagta ttttagcggt ctggttgaca taaacctccc tcttgaggtt
     3121 gtcaggtatc cttgctatgg attggaggat gtcctgtgcg agggtaacct ttttgattgg
     3181 atcattttta gcttcctcgg caacaccggc tattttaaaa aggatgaagt ccctggattc
     3241 ccgggttaca aaatccttca atgcctgtgc tccatgactc ttaaggtagg catccgggtc
     3301 atggccatcc ggcaggacgg caattttcac attgagattc tgctgcaata agatgtcgag
     3361 tccacgctgg gctgccttaa tgcctgctgc atccccatca tataataggg tcacattatc
     3421 cgtgaatctt ttgattagtt gggcctgttc tcccgtcaaa gctgtgccgg aggaggcaac
     3481 tgcaatctca attcctgctt ggtggagaga aattacatct gtgtaacctt ctacaaggta
     3541 acagttgtcc tccttccgta tagcgttctt gccctgaaag aaaccataca ggatcttgct
     3601 tttgttatag atgtcggaat ctatcgagtt gaggtatttc ggagccttct cgctgatggt
     3661 caatgacctg ccgccaaagc cgattattct tccggaaaga ttaaatattg ggaacatcac
     3721 ccgattgacg aaaaaatccc tgtcattttg ggttacaagc ccgatttgct tcatcaaatc
     3781 aatattgaat gcctttattt ttgcttcatt gattagatta tgaccatccg gaaaagcaaa
     3841 tcccaaaccg aatttttcaa ttgttacatc gtgaaaaccc ctttccctga agtaagataa
     3901 gccaatgttt ctgccatatt cagtttccgt aagattggaa atgtagtgtt tcatagcaaa
     3961 ctcattgatg agcaagaggc tttctcgctg tcttgccacc tcattttgtt ccggcgtccg
     4021 tgcttcctct tccacggtca cattgtattt tttggcgaga tacctcaatg catcaccata
     4081 ggccatgttt tcgtgttcca tgatgaagcc aaccggtgtg cccgagcgac cacagccaaa
     4141 gcatttgaaa atattctttc cgggagaaac tacaaatgat ggcgtcttct cattgtgaaa
     4201 ggggcagtgc ccaatcatat tgactccccg tcgctttaat accagaaaat cacctaccac
     4261 ctcttcgatg cgtgctttct ggaatatttc ttcaacggtc ttgcttggta tcatacaatt
     4321 ctaaagacat tcaactctgt atagttcaac tatcaaaggt aaaactttag aattggttga
     4381 aaaaaggatt ctatggaata ttgtgcttta attctccttt ctttcaaaaa ataagtataa
     4441 aagataggtt ctagcggata aacatcatct cccggtattt tgccagtggc caaagtccat
     4501 cttccactat ttcttccaaa tgatctactg attccctgat ctcagccatg attggcttaa
     4561 ccaaatggca gaacaattca gcttcttctt caggttcagc ttcagagacg gctttattaa
     4621 ccgactggtt tagtgtttcg accttttctg caacattatt aatatggtga gtaatttcat
     4681 tcaataagtt actttgaggc ctgaaagcgt cctcctgtag gccaagttgt tttaaggtat
     4741 aaatgttttc agctattccg gtctgatatt tataacatgc tggcaataca tatgacttgc
     4801 aaagatcaga aagcaccctg gcttcgattt ccagctttag attataattt tcgaccatta
     4861 cagtataatg agcctctagt tctatttcgc tgaatatgcc tgtcttttgg aagagctcac
     4921 ttgttttgtc catcttgtac actttcaaag catccggcgt attgggcgtg ttgctcaatc
     4981 cccttttctc tgcctctttt tgccattctt cactataacc atcgccttca aacaatatag
     5041 gaagggattc attgatatat ttccttaaaa tggacaaaat tgcggtgtcc atggattctc
     5101 ctttggatgt aagtagatcc aactcctttt taaattcagt caactgattt cctacaattg
     5161 tgttcattat ggtcatcggt gcagaacagt tcatggaaga tcctaccatc cgtatttcaa
     5221 atttattacc tgtaaaggca aatggagatg ttctgtttcg gtccgtattg tcaagttcaa
     5281 gattaggaga tttcttaagc agtgaaagca cctccttcac cattgttttg tcactttctt
     5341 tgccttccgc aataaaatgc aacacttctg ttagagcttt tccgacataa acggaaataa
     5401 tggcaggtgg agcttcattg gctcccaatc tgaaatcatt ggatgcagat gcaacacttg
     5461 ccctaagaag atctgcatgc tctttaactg ctctgatcgt attcacaaag aaagtcagaa
     5521 acttaaggtc ttttcccgga tttttacccg gagaaaggag gttcttcccg gtattggtcg
     5581 ctattgacca attgttgtgc ttgccggatc cattgacacc attgaatggt ttttcatgca
     5641 acaataccgc tagtttatgc cttttagcga cacgttccat gatgtccata agtaattgat
     5701 tgtggtcaat ggcaatattg acctcctcat acattggggc caattcatat tgaccgggac
     5761 ctacttcatt gtggcgtgtc ctgaccggaa tgcctaattt gtgacattct gtttccagat
     5821 ccctcatata gttaaggact ctttcgggaa tagatccaaa atagtggtct tccaattgct
     5881 gccctttgtg gctttgccgt cccagtaatg ttcttcctgt tatcataagg tcaggtctgg
     5941 caataaggaa agattcatca accagaaaat attcctgttc ccaacccaga gtggcgtggg
     6001 ttctttcaat gctttcatca aatatcttgc atacctccaa agcagcattc tccagataag
     6061 ctactgattt tagcaggggt aatttgtaat caagggattc accactgtag gtcacaaaaa
     6121 tagttggaat acacagtgtc ttcccattcc caacttctat aataaatgcc ggtgatgatg
     6181 gatcccatga agtatagcct cttgcttcaa aagtacttct cagtccaccg ccgggaaaag
     6241 aagaaccatc cggttcctgt tgaaccaatt gttcacctcc aaattcctca atggcttttc
     6301 catctgctgt aatggtaaag aatgaatcgt gtttctcggc agtcttccct gtcaaaggct
     6361 ggaaccaatg ggcataatgc gtcgcacctt tgtccatggc ccaattcttc atggcttcag
     6421 ctaccgaatt agctacttcc ttggtgaggt tagttcctga ttgaattgct gccttcacac
     6481 taaaataggc tgattcagaa agatatttct tcatcgcttc atcatggaat acattctcgc
     6541 cataaaatcc tgagatttta accttgtcag gcgtgtttac aggaaaatcg atatcgaatc
     6601 tattgtgtat aatatcgatt gcatttgacc ttttaagact cattttgttt gaaaattgat
     6661 tatttggaaa ataacattag gaatgaccgc aaagataatt acaaaaattt ttttctaacc
     6721 tgttatatat aataaatttt ataacttatc ttgccgttat tatggacaag ttttgaaatg
     6781 gcttgcagga cttgattttc tgtattttca acttaactta tccaggttgc attaattaaa
     6841 ctttaacaaa gttgaggtat ttaaaacttc tgatttttag tttgctcata cttttttgtc
     6901 ttcctttgga ggcacaagtg gacggttcaa ctgttgatat tgaggtgata gatgaagtga
     6961 ttgaatcaat acttgcttcc aatccggacc ttacggaagc ccaaatcaat gatcttaccg
     7021 atcgcttgtc tgttcttgcg cagagtaaag tcgatcttaa tgaatccgat ctgaaggtat
     7081 ttgaagaact acttttgtta aatgaacttc agatacgtga gattttgttg catcgggaag
     7141 tgtatggtga ctttattgca ttatatgaac tgcagactgt cccttctttg acactttccg
     7201 atatccgcag aatatggcct tttttgaagg gaataggaac accgggcata tcgccgtttt
     7261 catggtctat gctttacaag ggaagtaaaa ccataatatc ccgttattcc agagtgcttg
     7321 agcaagcttc aggatatgtt ggtgattctt ctaaatatct tggaagcagg gatcagataa
     7381 gttttaggat gcgtcatgtt gttcctggac gtgtgagcat tggaatcgga gttgaaaaag
     7441 atgccgggga gcccttgaat acaaattata atcccttttt attcgatttt gttacctttc
     7501 atgcctatct tgagaatgtg gcgaagcaaa ttaaacagct ggctttaggt gactttcagg
     7561 tttctgccgg gcaaggatta atcatgtttt cagggtatgg ttttggaaga aatgctttta
     7621 ctacagccgt aaaaagaaac gaaagggcga tcagaccttc tacttcattg aatgaaatta
     7681 atgctctgcg tggcgctgct gtgggaattg agttggcccc cggatggcag atgatagcct
     7741 ttgcaagcct taaaaaaagt gatggtactt taataattcc tgattcatta tccggtgatt
     7801 ctgatgtgct tatttcttct ttgcagatgt cgggattgca tcgtactgaa agtgaaatta
     7861 ggaatggcaa acatatcagt atgtttacga ccgggggatc tttgaaatat aacggtaacc
     7921 gatttcaatt tggagcaaat gccgtttatg atcaacttga taaaagagtt gagccagccg
     7981 atcgagttta taaccggttt tattttaaag gaaaaaatgc cctgaatgcc agtgtcgatt
     8041 atacatatta tttccgcaac ctgatcttct atggtgagac agcaattaac cctgagaatg
     8101 cagtagcaac tgtcaatggc ttattagtgg gtttgcaccc gaaggtcaat ttagccttat
     8161 tgtttcgaca tttgccaaag gaatattaca gtcttcatgg aaaagtattt tccgaccaaa
     8221 gccagacaac caatgaaaca ggattttatg ctggactgga agtaaagcca agtccgcgat
     8281 tcttagtcaa catatatgct gacttctttc gacatggatg gcctaccttt agtgcaaatg
     8341 gcccctctga aggaagcgct tatctattta aggca
//
LOCUS       DAOQMC010000091         8343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3426_length_8343_cov_5.384653, whole genome shotgun sequence.
ACCESSION   DAOQMC010000091 DAOQMC010000000
VERSION     DAOQMC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_8343_cov_5.384653"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..74
                     /locus_tag="PKV31_05040"
     tRNA            1..74
                     /locus_tag="PKV31_05040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Arg,seq:tcg)
     gene            complement(109..564)
                     /locus_tag="PKV31_05045"
     CDS             complement(109..564)
                     /locus_tag="PKV31_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB92257.1"
                     /translation="MNDPLFIENEIEINAPKEKVWQALTDPTVTKKYMFGCEVISSFK
                     EGDSMVWEGDVEGERIMFVKGNIVKCDINHELVYTAFDPTSGAEDIPDNYLTITYRLT
                     ESAGKTTLFVSQGDFAKVHDGEVRFQSSMDNGGWIGQLMSIKQLLEEGE"
     gene            complement(569..1168)
                     /locus_tag="PKV31_05050"
     CDS             complement(569..1168)
                     /locus_tag="PKV31_05050"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNB92258.1"
                     /translation="MFLEPHHKGQRSGWIEVICGSMFSGKTEELIRRLKRAQFAYQKV
                     MVFKPSRDIRYDEVNVVSHDSNIIGSTPVRFAREIIELSEDYEVIGVDEAQFFDNDIV
                     DVCQRLAFIGKRVIVAGLDMDFAGKPFGPMPALLAVSEYITKVHAICVHCGNLATHSY
                     RLSDDAEQVLLGAKDLYEPRCRVCYSEGNLINFNNPVKD"
     gene            1282..2304
                     /locus_tag="PKV31_05055"
     CDS             1282..2304
                     /locus_tag="PKV31_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92259.1"
                     /translation="MHNSKSAEYLDKINKAFAQLNNPPHEIEIDSLLGYIRIFYSSIK
                     QLPIDDSLPIDAEKEISSIDPFEKKAEVNDLPPIIKPERETTQINESKEDLVEPPAEV
                     ESSVPSDEASNDSVVQKEAGPEIKPEELEIADEPQVPEEIGQVNDTHEDTLVIEDIPY
                     VEIEAVSQQNQANAPDHIKKVSPPVEEQPFVRDNTTVEVPIIQTKAPVKEESMLTRIE
                     TQQNEPRYIPANERDFDTSDAVESIFRDKSPTGLVDFLGLSPLSDLNKAWGLNERMLI
                     IKDLFGDDLNMFNQTIEAINKCTSFAEAKSWLIQNIVNQFEWTDLARYKKAINFVTQV
                     KRLFVK"
     gene            2399..3109
                     /locus_tag="PKV31_05060"
     CDS             2399..3109
                     /locus_tag="PKV31_05060"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB92260.1"
                     /translation="MEREKSALLSSIKFSLKLGFVLFLTYLITLVLPIDIGYLGIIPR
                     EKYGLLGILTGPMVHGSWSHLLSNLPPLLVSVFLIHYFYKRQFWSIFLLSYILTGVMV
                     WSFGRDVSHVGISGVVYALIAFIFWSGIFVRNLTSIILSLLVLVVYSGMFAGILPSPE
                     IMAKNISWESHLLGGLVGIAVAWWQKESIIAEHKAKEVPRSYSVDEPRQPYFPQDTFE
                     KTRWQRYLEEQEASRREE"
     gene            complement(3348..7544)
                     /locus_tag="PKV31_05065"
     CDS             complement(3348..7544)
                     /locus_tag="PKV31_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB92261.1"
                     /translation="MDGYCADMQGGAWGNNTPFCVGGSNGYTLQNPNWFAFVANSTTI
                     DLEIAMSNCTGSSNPSVQWGLYNQCTNLLGALICNGTGTGNGGVVNIVYNNASPGTIY
                     YIVLDGFSGATCHYEVTVNSGIGVTLVAPALNTDLTGMANVCVGATVDYSFAGFGFAT
                     QYDWTLPDGANVSTTGPSTSVSFANVAPGTYQLCVTGTNECDDIGQSLCWSIEVEAKP
                     ELEEYGEVCPGSTFNFRGGNYPAGTYALIYADVNGVSCDTTINLHVTEYIPTTTPNQQ
                     IFICPGEEFAFIGGVPITEGQMGNILTFQDQHGCDSLVTYFVNELEVYGMIDAVPASL
                     PCTGGTSTMTLNPNWGFFTTDTYGIEWYDSNDALLGTGNSIQITKPGYYYAIVTWYKT
                     NNVLPGTNLDPMTLLCDDRFEITIPAENSTLAEPVVTGSTVLCAGEAYTFDTDTQTGT
                     TSYNWIHTGGTVNSGGNNEHIELTYATPGQYEICVNAVDGCGPGPDNCFQIQVVPSAT
                     VDLGSDRTVCGNIDTLRAVLGGVPDVNDPNMQYIWTVVSGPDVNGVTFSAGNKAETQV
                     DVVQAGTYTFQFGADYNGQGCGEADTIEVIFEKALVLTPKDIEVCNDENQPLPNEINL
                     DTLLTGNGVAYTGTWTMVSGPGTPGGTLPVQNYKGMTQTGVYVYRFTPNQSGVCGVSA
                     VEVKIDLKNCICPPLSINSDGGTTCNDAGSVNLGTMVQGATGPGKWAVTTQPTGGGVV
                     LSGSLADFKGQPAGAYVFTYTLDDTMTGCPSSATTTVNVVSAPQAQLKVAEQACNSNF
                     SPDYVNEVNFDTLIVSGDKTGVWTYSGVGVDPGSASFYPKDFNGAVPGTYEYTYTITG
                     DPSCAPVSYKINIVVEDCKCPSVAITPFASFCNTVATVDLDSYRVTTKPGKWYIESVP
                     AGSNATITGDRMFNGAGSVFGTYVLYYKLDEVVPLGCEDTSQLVIRLDSTASAGIDGL
                     IGRCEDYSGSIDLDTVLTQQQAGGTWQYTGSVNIGSSFNAGAGILDVSGIPTGLGYTF
                     KYTVKSPLGLCPDDNATITVDRNELPNADAGVDGFIDCVTTAVDLGGSGTSSGAEYSY
                     EWTNLTTGSKVGTTAVLTGVQDGGVYELKVTNATTGCVKVDEVEVRKDDKAIDGISIE
                     LDSISCYNKGDGEVRIIGVTGGTPDFRYSLDGSAYTAQSVYSNIGPGSHIIRVEDVNG
                     CKYDLPILLDNPKEVKVELGNNKVIVEGDAVTITPDVSIGVNGAAIDWTSNPPGTSCV
                     GCAELTVQPLITTTYIATVRDKNGCTAVDSMEVRVKSVIRVFIPNVISPNNDGINDVF
                     YVQTDQNIVRVKSMTIYNRWGDVQYSVKDVPPNVESQGWNGRYGEQRSNTPAVFVYQI
                     VLVTREGREITYKGDVTVVR"
BASE COUNT         2354 a         2220 c         1461 g         2308 t
ORIGIN      
        1 ggctccgtag ttcaattgga tagaatatca gatttcggct ctgagggttg agggttcgaa
       61 tccttccggg gtcacacaaa agcctgccaa tttatatggc gggctttttt attcaccttc
      121 ctcaagcaac tgcttgattg acatcaactg accaatccat ccaccattat ccatggagct
      181 ttgaaaacga acctctccat catgcacctt ggcaaaatct ccctgcgaga caaataaagt
      241 agtcttccct gctgattctg taagtcgata ggttatcgtg agataattgt ccggaatatc
      301 ctcggcgcct gaagtcggat caaaagcagt atagaccaat tcatggttga tatcacactt
      361 gacaatattg cctttgacaa acatgatacg ctcgccctcc acatctcctt cccaaaccat
      421 agaatctcct tccttaaaac ttgagatcac ttcacaaccg aacatgtatt ttttggtcac
      481 tgtaggatcg gtcagcgctt gccaaacttt ttcttttggc gcgttgattt ctatttcatt
      541 ctcaatgaaa agtggatcat tcatgatatt aatcctttac cgggttatta aaattgatta
      601 aattaccttc cgaatagcag acacggcatc ttggttcata caaatccttg gctccgagca
      661 acacttgctc tgcatcatca gaaagtctat aggaatgtgt cgccagattg ccgcaatgta
      721 cacatatggc atgtaccttt gtaatatatt cagaaacagc aagtaatgca ggcatcggac
      781 caaagggctt gcctgcgaaa tccatatcaa ggcctgctac aatcaccctc ttcccaataa
      841 aagcaagcct ttggcataca tccacgatat cgttgtcaaa aaactgggct tcatccacac
      901 caatgacttc gtaatcctca ctcaactcaa taatttccct ggcgaatcta accggagtgg
      961 aaccgatgat attcgaatca tgactgacta cattgacctc atcatatcgg atatcacgac
     1021 tcggcttaaa caccatcacc ttttgataag caaattgagc tcttttcaat ctccggatca
     1081 attcttccgt ttttccggaa aacattgacc cacatattac ttcaatccaa ccgctgcgct
     1141 gacctttatg atggggctca agaaacattt atcctataat ttatatataa ttattttatt
     1201 taatgtattt ttgtaacgat aaaaatggaa tatgatttta ccattctttt tcgtttaata
     1261 aagcataaaa ttaagcaaag tatgcacaat tcaaaatcgg cagaatatct ggataaaatt
     1321 aataaggctt ttgctcagct taataaccct cctcatgaga tagaaattga ttccttgctg
     1381 ggttatatcc gaatattcta ttcatcaata aaacaattgc cgattgacga ttcattgcca
     1441 attgatgctg aaaaggaaat atcttccatc gatccgtttg aaaaaaaggc tgaagtcaat
     1501 gatctgcctc caattatcaa gccggaaaga gagactaccc aaataaatga aagtaaggaa
     1561 gacttggttg agcctccagc agaggttgaa agcagcgtcc catctgacga agcatcaaat
     1621 gacagtgtgg tacagaagga agcaggtcct gaaataaagc cggaagaatt ggagatagca
     1681 gatgagccgc aagtaccgga agaaatcggg caggtcaatg atacacacga agatacactc
     1741 gttattgaag atatacccta tgtggagatc gaggctgtaa gtcaacaaaa tcaggcaaat
     1801 gcgcctgatc atattaaaaa agtatctccg ccggttgagg aacaaccatt tgtccgtgat
     1861 aacaccacgg tggaagttcc aatcatacaa acaaaagcgc cggtcaagga agagagtatg
     1921 ctgaccagga ttgaaacgca acaaaacgaa ccacgataca tacccgccaa tgaaagggac
     1981 tttgatacct ctgatgctgt ggagtctatt ttcagggata aaagtcctac aggcctggtg
     2041 gacttccttg ggttaagccc actcagtgat ctgaataaag catggggact gaatgaaaga
     2101 atgctgatca taaaggactt gtttggtgat gatctgaaca tgtttaatca aaccatcgaa
     2161 gcgatcaata aatgtacaag ttttgcagag gcaaaatcct ggcttattca aaacatagtt
     2221 aatcagtttg aatggactga tttggcaagg tataagaaag cgataaattt tgtgacccag
     2281 gtaaaacgat tattcgttaa ataaacagaa cataccgact ttggatcaaa acctgtttca
     2341 gtcaagagca aataaacttt tttggcatga atttcgtttt cgtaaaaaat gattagttat
     2401 ggaaagggaa aaatctgccc tcttatcaag tatcaaattt tctctgaaat taggatttgt
     2461 tctttttttg acttacctga taacattagt tttgcctatc gatattggtt atttaggcat
     2521 tataccaaga gaaaaatacg gcttgctggg aattctgaca ggacccatgg tacatggaag
     2581 ctggagccat ttgctttcaa atcttccacc cttgctggtt tcggtatttt tgattcatta
     2641 tttctataaa aggcaattct ggtctatttt tttgctgagc tatatcctta ccggagtaat
     2701 ggtatggtcg tttggaaggg atgtttccca tgtaggcatt agcggtgtag tgtatgcctt
     2761 gatcgctttc atcttctgga gcggtatctt tgtcaggaat ttgacatcaa ttatcctttc
     2821 tttactggtt ttggtggttt atagtgggat gtttgccgga atcctaccat ctccggaaat
     2881 catggcgaaa aatatcagtt gggaaagtca tctattaggc gggttggttg gcattgccgt
     2941 agcatggtgg caaaaggaat caataattgc agaacataag gcaaaggaag ttcccagaag
     3001 ttatagtgtt gatgagccaa ggcaacctta cttcccccag gatacttttg aaaagaccag
     3061 atggcaacga tatctcgagg aacaagaagc ttcccgacgg gaggaataag gtcgaaatat
     3121 acaatactcc ctactgatac aggacatatc cctaaaaata aaaagagcct gtttggctct
     3181 ttttatttta tttacaccga ctaagtcggt caccatttat ccaatcataa agtattccaa
     3241 accatcaccc tcctctgcag atccttcatc gcacaggatt gccgcttcga tcacctcttg
     3301 aaggcctctt ttacattcgc cttcctcttt tctcttgcta tcctttctta acgcaccaca
     3361 gtaacgtcgc ccttgtacgt tatctcccgc ccctcgcgtg tcaccaatac tatttgatac
     3421 acaaacaccg caggcgtatt cgaccgctgc tctccataac gaccattcca cccctgtgac
     3481 tccacattcg gtggcacatc cttcacagaa tattgaacat ctccccacct gttgtatatt
     3541 gtcatactct ttaccctcac tatgttctga tccgtctgca cataaaatac atcattgatc
     3601 ccatcgttgt tcggactaat cacattcgga ataaacaccc ggatcacact cttgaccctt
     3661 acctccattg aatctactgc cgtacaccca ttcttgtccc gaaccgtagc tatataggtc
     3721 gtcgttatca atggctgtac cgtcaactca gcacaaccaa cacaactcgt ccccggagga
     3781 ttcgacgtcc agtctatcgc cgctccattg acccctatcg acacatccgg cgtaatcgtc
     3841 accgcatctc cttccactat caccttgttg ttgcccaact ctacctttac ctccttcgga
     3901 ttgtccagca atatcggcaa atcatacttg cagccattta catcctctac ccggataata
     3961 tgactgcccg gccctatatt gctgtatacc gactgtgccg tatatgcact gccatccagt
     4021 gaatacctga agtccggcgt gcctcctgtt acccctatta tccgcacctc tccatctccc
     4081 ttattataac aactgatgct gtccaattca atgctgatcc cgtctatcgc cttatcatcc
     4141 ttcctcactt ctacctcgtc cactttcaca caacccgtcg tcgcgttcgt cacctttagc
     4201 tcatacactc ccccatcctg aactcccgtc aacaccgctg tcgttccaac cttactccct
     4261 gtcgtaagat tcgtccactc ataactgtac tccgccccgc tcgacgtacc gctacccccc
     4321 agatctaccg ccgtcgtcac acaatcgata aacccatcca ctcccgcatc ggcattcggc
     4381 aactcattcc tgtcaaccgt tatcgtagca ttatcatccg gacacagccc caacggactc
     4441 ttcaccgtat atttgaacgt atagcccaat cccgtcggta ttccgctcac atccagtata
     4501 ccggctcccg cattaaacga actgccgata ttcactgacc ccgtatattg ccacgtaccc
     4561 ccggcttgtt gctgtgtaag taccgtatcc agatcaatcg atccggaata gtcctcacat
     4621 cgcccaatca aaccgtctat ccccgcactc gccgtactgt ccaagcgtat caccaactgt
     4681 gaagtgtctt cacagcccaa tggtaccacc tcgtccagct tataatataa cacatacgtc
     4741 ccaaacacac tgcccgcccc attgaacatc ctgtctcctg taatcgtcgc attgctgcca
     4801 gcaggaacac tctctatata ccacttgccc ggctttgtcg taacccggta actgtccaga
     4861 tccactgttg ccaccgtatt gcagaatgac gcaaaaggcg tgatcgctac cgatggacac
     4921 ttacaatcct ctaccactat gtttatcttg tagctcaccg gagcacaact cggatcgccc
     4981 gttatcgtat aggtgtattc ataggtgccc ggtaccgccc cgttgaaatc cttcggataa
     5041 aacgatgcac tgcccggatc aaccccaaca ccactatacg tccacacccc cgtcttgtct
     5101 ccgctaacta tcaatgtgtc aaagtttact tcatttacat agtccggcga aaaattactg
     5161 ttgcacgcct gctctgccac cttcaactga gcctgtggcg cagacaccac attcaccgtc
     5221 gtcgtcgccg atgatggaca tcccgtcatc gtatcgtcaa gtgtataggt aaacacatat
     5281 gcccccgcag gctgaccctt gaaatccgcc aacgagcccg acagcaccac tcctccaccg
     5341 gttggctgtg tcgtcactgc ccatttaccc ggccccgtcg ccccttgtac catcgttccc
     5401 agatttacac tgcccgcatc attgcacgtc gtacctccat cactgttgat gctcaatggc
     5461 ggacatatac aattcttcag atctatcttc acttctacag cactcactcc acatactcca
     5521 ctctgattcg gcgtaaaacg atatacatat actcccgtct gtgtcatccc cttataattc
     5581 tgcaccggca acgtgcctcc gggcgttccc ggtccactca ccatcgtcca tgtacccgta
     5641 taggcaacgc cattcccggt caacaacgtg tcaagattaa tctcattcgg caatggctga
     5701 ttctcatcat tgcacacctc tatatccttc ggcgttagta ccagcgcctt ctcaaatatc
     5761 acctctatcg tgtcagcctc tccacatccc tgcccattgt aatccgcccc aaactgaaac
     5821 gtatatgtcc cggcctgtac tacatcaacc tgtgtctcgg ccttattccc agcactaaac
     5881 gtcactccgt taacatcagg ccccgatacc accgtccaga tatactgcat gttcggatca
     5941 ttcacatccg gcactccccc caacactgct cgcaacgtat caatattgcc acatacggta
     6001 cgatcactcc ccaaatcaac cgtcgctgat ggtacaacct gtatctgaaa acaattgtcc
     6061 ggcccgggcc cacatccatc taccgcattc acacatatct catactgccc cggcgttgca
     6121 tacgtcaact cgatatgctc attattacct ccactgttta cagtacctcc cgtatgaatc
     6181 cagttatacg aggtcgtccc cgtctgcgta tccgtatcaa acgtgtaggc ctctcccgca
     6241 cacagtaccg tcgaccccgt taccacaggc tctgccaatg tcgaattctc cgccggtatc
     6301 gttatctcaa accgatcatc acacaataag gtcatcggat ctaaattcgt ccccggcaat
     6361 acattgttcg tcttatacca tgtcactatc gcataataat atcccggctt cgttatctgg
     6421 atagaattac ccgtccccaa caatgcatca ttactatcgt accactctat cccataggta
     6481 tccgtcgtga aaaatcccca attcggattc aacgtcatcg tcgatgtccc tcctgtgcac
     6541 ggcaatgacg caggcacagc atcaatcatc ccatatacct ccaactcatt gacaaaatac
     6601 gtcaccaaac tgtcacaccc atgctgatcc tgaaacgtca atatattacc catctgccct
     6661 tcagttatcg gaacccctcc gataaatgca aactcctccc caggacagat aaatatctgc
     6721 tggtttggtg tagtcgttgg tatatattcg gtgacatgaa gattgattgt tgtatcacaa
     6781 cttaccccat tgacatcggc ataaattaat gcataagtac cggcaggata attgccaccc
     6841 ctgaaattaa aagtgcttcc cgggcagact tcgccatatt cttctaattc aggtttagcc
     6901 tcgacctcta tggaccaaca taaactctga cctatgtcat cacactcatt ggtaccggtg
     6961 acacataatt gataagttcc gggtgctaca ttcgcgaagc ttactgaggt tgaaggcccg
     7021 gttgtagaga cattagctcc atcaggcaag gtccaatcat actgtgtggc aaaaccaaag
     7081 cctgcaaaag agtaatcaac tgttgcacct acacagacat tagccattcc tgtaaggtca
     7141 gtattcaaag ccggagcaac caatgtcact cctatgcccg aattgacagt aacctcataa
     7201 tgacaggtag ctcccgaaaa cccatcgagt acaatataat aaatggttcc gggcgatgca
     7261 ttattatata cgatattgac aactccacca tttcctgttc ctgtaccatt acatatcagc
     7321 gcacctaaaa ggttagtgca ttgattatac aatccccatt ggactgaagg attagaacta
     7381 cctgtgcaat tactcatagc aatctccaga tctatcgtcg tactattagc tacaaaggca
     7441 aaccagtttg gattctgcaa ggtatagcca tttgagcccc ctacacaaaa tggcgtatta
     7501 ttcccccatg cccctccctg catatcagca caatatccat ccatatcgca aacaaatgga
     7561 gctgttgggc atgtctgacc agggttaata tttaaatcag ggttgcaatc agttattgca
     7621 atcggcccgg caaaagaagg atttgttata gggatcagct cttcaatccg aatttgagcc
     7681 gaaagtgata aggatttaat taacaataat tgaagtataa ataaataatt cttcataaac
     7741 aacgataacc ttataataat caacaaatat actgaagatg atgaatattg aaaaacaagt
     7801 tcttccttat acgctcaaaa tcgacataaa acactccact cagccattaa attatctatt
     7861 tattcgcaac tattcacaaa tcactgaccc acattcttct aaataattcc aggattagta
     7921 aagcctgaaa aacttgcact actgaacctc ttctaaacta gttctgccaa ttgagttagc
     7981 tatgacaaaa gcctttatac cccttatgcg ttttgaacta ccaaaaaaat gaaacttcta
     8041 ttttattaac cttcgataag gtaaaaaaca ttatcctctt tgtgtcactt tgtgtattcc
     8101 gagttgacaa aaaaaataaa ctgctgcatt tgtcgtaaaa agaattaccc ctgtaaaatt
     8161 gaaatagatc atgacattgc aatcttttgt ccgatttaat aaacctctga taatcttttg
     8221 aagaagtgaa tagttcgatt tactgataaa ataaaaagag cctgttgggc tctttttgtt
     8281 ttattgatac cgactaagtc ggtcaccatt tatccaatca taaagtattc caaaccatca
     8341 ccc
//
LOCUS       DAOQMC010000092         8329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3436_length_8329_cov_4.636693, whole genome shotgun sequence.
ACCESSION   DAOQMC010000092 DAOQMC010000000
VERSION     DAOQMC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3436_length_8329_cov_4.636693"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="pepT"
                     /locus_tag="PKV31_05070"
     CDS             complement(<1..720)
                     /gene="pepT"
                     /locus_tag="PKV31_05070"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripeptide aminopeptidase PepT"
                     /protein_id="HNB92262.1"
                     /translation="MSLPKFNYTVTDRFLHYVTIDTQSDPGSPTTPSTQKQKDLGRVL
                     VSQLLEMGLTDAHMDEHGYVYATLPSNVDHEVPVICFCSHMDTSPDCSGTDVKPIIHR
                     NFDGGKIVLPLDNSQVITIDGHPALADQIGNDIITASGDTLLGADNKSGVAEIMDAVF
                     QMIQHPEIKHGKLRILFTPDEEIGRGVDKVDMVKLAADFGYTMDGETVGHVEDENFNA
                     KAAVVKITGVAAHPGFAKGKME"
     gene            1291..2055
                     /locus_tag="PKV31_05075"
     CDS             1291..2055
                     /locus_tag="PKV31_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNB92263.1"
                     /translation="MKIYSWNVNGIRASMGKSFLEKFNAFDADIFCLQETKAQEDQVE
                     KALEEVQGYHLYANSAEKKGYSGVAILTKQKPLSFQKGIGVDVHDMEGRVLTLEFDNF
                     YMVNVYVPNSGDQLVRLEYRAEWDDAFSSFLAALRRQKPVVVTGDFNVAHTQIDLARP
                     KENYNKTSGYTQVEIDGMDLILNAGFSDAFRKLYPEKIQYTFWSMRFGARAKNMGWRI
                     DYFLVDKDIDSKITECTIHDQVMGSDHCPISLNIDL"
     gene            2096..2596
                     /locus_tag="PKV31_05080"
     CDS             2096..2596
                     /locus_tag="PKV31_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92264.1"
                     /translation="MTLGEFIDWIGRDPKYIICFFLLMPVGAALMWFIAKDKGHISPW
                     KYIYSILIYSVCIPGVSAVAFNLYLFVFQRQDVFAMDIFTQVMPILSMVLTLWLVRRN
                     VAFESIPGFDKLSQLITIIFAVLMLLWLGDRVRIYSITFVPLWMFILIFLGLILLIRY
                     AWSKLF"
     gene            complement(2676..2918)
                     /locus_tag="PKV31_05085"
     CDS             complement(2676..2918)
                     /locus_tag="PKV31_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNB92265.1"
                     /translation="MSQSIEEKVRKIIVERLGVDESEVTLEASFTNDLGADSLDTVEL
                     IMELEKEFNISIPDEQAEQIQTVGDAVSYLESQINA"
     gene            complement(3170..5002)
                     /gene="uvrC"
                     /locus_tag="PKV31_05090"
     CDS             complement(3170..5002)
                     /gene="uvrC"
                     /locus_tag="PKV31_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNB92266.1"
                     /translation="MKKEAFREISPSIPREPGVYRFMDKDDNIIYVGKAKNLKNRLTS
                     YFNSTNKAGKTRIMVDHADHLEYTVVETEHDALLLEATFIKKFQPRYNVMLKDGKSYA
                     YICIRKEPFPRVFFTRKVIKDGSTYFGPFTSKFRVQQILDLIKNLFPLRTCNLNLTPE
                     NIRKGKFKVCLEYHLKNCMGPCEGLESEEAYNAKIDQIKNIMRGNFAPVKAYLREQMD
                     AFAENLEFERAAEMKNKLSLFEDYQAKSMVVSTTIRDVDVFAYHAEENEFYINYIKVV
                     NGMMINTFLQEGIRNLDDDAFNFSYGIRQLRERFNSIAPEVLVNMEGVILDEKELKVQ
                     IPSIGDKRKLMEMAEKNIHFYLLQKRKESINAPRVTPTQRIMETLQKDLNMDRLPNHI
                     ECFDNSNIQGTNPVSSCVVFKNAKPAKSEYRKFNVKTVDGPNDFATMEEVVTRRYTRL
                     LEEGQPLPDLIIIDGGKGQLGVAAEVLTKLGILGKVTLIGIAKRLEEIYFPGDSLPLY
                     INKKSESLKLIQHLRNEAHRFAITFHRKKRSMNFTVSELTQIDGIGKATAEKLLQHFK
                     SVKRIAAASPEELAGVTGKRAANIVFKYYNRAEEEGEEEVPEVG"
     gene            complement(5018..6064)
                     /locus_tag="PKV31_05095"
     CDS             complement(5018..6064)
                     /locus_tag="PKV31_05095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNB92267.1"
                     /translation="MSESIQRAIEHFFPETGSFTLLPIKSGLINHTWKLTFDSSSFVL
                     QQVNKDVFTDPELIHGNLDLLRTYLARTVKDVHLVLPLSDQDDRSLFFDGESYYRIFP
                     WVEDSVTVSVVNSPEQAYEAARQFGRFTAAFHGFDHGLLKPVLPDFHNLRLRFDQFHH
                     ALKSGNPERIESSKEIIDQLLQKNRLVDIHDRMVASEQFRVRVMHHDAKISNVLFDRN
                     DKGICVIDLDTVMAGRIFSDWGDMVRTYVCPVSEEETELDRITIRKEYLEAIENAYLE
                     ELGDVMTEPEKKSLRISGMILIYMQALRFMTDHLHNDVYYGAAYPGHNFNRAVNQFML
                     LTALENSGDSTEWL"
     gene            6539..>8329
                     /locus_tag="PKV31_05100"
     CDS             6539..>8329
                     /locus_tag="PKV31_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92268.1"
                     /translation="MSGKGRQATILLSTAFLLLASIGIWTYVSQKNIEKRQIADIALR
                     FQKLERKAQESIDNKEIIDFLSQWSEGTYLTDKASEKLITKLATSPVVLGMEAGGRLT
                     AWTGNIHSQETGKSLLNGYYLIDDSIFQKKYPIATGQDIKVVVEFGLPGKFYKEKKDG
                     LTELKSGDGNLTAYFLPSPKQKSLSGDWKILPFYLAAYTLLLIGLSKRLKRKTKVPFI
                     WSIGSTLLIAIAYRLLDWFLGAGMSIDIGLINSGLTPKPILSEWFANWILILLIYFLV
                     IENVIDNLPPIKASKRKRNPLLLYTTGLYALDYLGFVGILVFCRELVLRSGINFDFSN
                     VLNLGVGPLISLVLLALLLFRYFVFAYKINLVLYKNRIKLNNKLIGMIGGFLLMLPWL
                     LLLRLDLPLFILILVVLLFALLFDIFISTPSPSLGWLVIWIFFFSSFATIALFKFNRD
                     KDHDDRLALINILKNKEDSLLLRQFGEFVNNLKNDRQNNAAMTEALLQGSSISSSNVI
                     QNVLDEFEYIRQFYNVEFFVAQNDSLLQESIPGLKNNFVSQGVKFSEAGPYLIAGRRK
                     TFHGYWLKIYWQGYPQRPSPTITFSVTKKLY"
BASE COUNT         2306 a         1852 c         1677 g         2494 t
ORIGIN      
        1 ctccatctta ccttttgcaa aaccgggatg cgcagccact ccggtaatct ttaccacggc
       61 cgctttagca ttgaagtttt cgtcttcgac atggcctaca gtctcaccat ccatcgtgta
      121 accaaagtct gcggccaatt taaccatatc gaccttatcc actcccctgc cgatctcctc
      181 atccggcgta aaaaggattc taagtttgcc gtgcttgatt tccggatgtt gaatcatctg
      241 aaatacagca tccataattt ctgcaactcc cgacttgttg tcagcaccca acagggtgtc
      301 accactggca gtaatgatgt cattacctat ctggtctgca agagccggat gtccatcgat
      361 ggtgattacc tgactattgt ccaatggcag gacaatctta ccgccatcga aatttctatg
      421 gatgatgggc ttcacgtcag tccctgaaca atccggactg gtatccatat gcgaacaaaa
      481 acagatgaca ggcacttcat gatccacatt ggatggaaga gttgcatata catagccgtg
      541 ttcatccatg tgagcgtcag tcaatcccat ttcaagcaac tgactcacca aaaccctacc
      601 gagatccttt tgcttctggg tgcttggagt cgtcgggctg cccggatccg actgcgtatc
      661 aatagttaca taatgaagaa atcggtctgt gacagtataa ttaaacttcg gcaatgacat
      721 atttatcaga attttgattt gatcggtgaa attaagataa tgttgagaat aatgagggaa
      781 aaatgtattc ttaaaaaaag tataaccata gacacaagtt gcacacaaac caatcgatta
      841 aatgaacaaa ctgaccaaaa acgcccatta tcccgataaa aaaggcatac ctgacaaaat
      901 aaaattacct accacacaaa atattatcag aaatcctgat ttgagatctc tccttctctg
      961 acctgatatt ccttttatcc ttagttaatc gtttgaatat tggtcttata attcctgttg
     1021 tgccggtgaa atcatccatt gtataatcgg attcatcaaa ccatctttta tttttaagtg
     1081 tcctgttaca gctaaaattt tttacatata tactctacct tatgaccggg gtaaagaagg
     1141 gtttcaatag ttgcttatgt attggtttgg acaagttgcc cggccccatc accactttga
     1201 tgcctatgga atattgatcc ggatacttct atttttgact acccaatttt catcaacttt
     1261 caccatattt acataacaat acaatcgaac atgaaaatat acagctggaa tgtcaacgga
     1321 atccgcgcct caatgggcaa gtcgtttttg gaaaaattca atgcctttga tgccgatatc
     1381 ttttgtcttc aggagaccaa agctcaggaa gatcaggtag aaaaggcttt ggaagaagta
     1441 cagggatatc atctgtatgc caattctgcc gaaaagaaag gctatagcgg agttgccatc
     1501 ctgacaaaac aaaaacccct ttccttccaa aaaggcattg gagtggatgt ccatgatatg
     1561 gaaggccggg tgctgacctt ggaattcgac aacttttaca tggtcaatgt ctatgtcccc
     1621 aacagtgggg atcagcttgt ccggcttgaa tacagagctg aatgggacga tgccttctca
     1681 tcctttctcg ccgctcttag gaggcaaaag ccggttgtgg tgaccggtga tttcaacgtc
     1741 gcacacaccc aaatcgatct ggcacgcccg aaagaaaact ataacaaaac cagtggttat
     1801 acccaggtag agattgacgg catggacctg atcctcaacg cagggttttc tgatgccttc
     1861 cgcaagcttt atccggaaaa gatacaatat accttctgga gtatgcggtt tggagccaga
     1921 gccaagaata tgggctggcg tatagattat tttttggtgg acaaagacat agattcaaag
     1981 attactgagt gtaccattca cgaccaggta atggggagtg accactgccc tatttcattg
     2041 aatattgatt tataacaata aaactaattt atataaccct attaataagc tagttatgac
     2101 tcttggagaa tttattgatt ggataggtcg cgatccgaag tatatcatat gctttttctt
     2161 actgatgccg gttggagcag ctttgatgtg gtttattgcc aaggacaaag gacacatcag
     2221 tccctggaaa tatatctaca gcattctgat ttactccgta tgcatccccg gtgtgtcggc
     2281 cgtcgccttc aatctctacc tttttgtgtt tcaacgccag gatgtatttg ccatggatat
     2341 attcacccag gtcatgccga ttttgtctat ggtcctgaca ctatggctgg tacggaggaa
     2401 tgtggctttt gagtcaattc caggctttga taagcttagt cagctgatca ccatcatttt
     2461 tgctgtgctg atgttattat ggctgggaga cagggtgagg atatattcga tcactttcgt
     2521 accgctctgg atgtttatat taatttttct gggtttgatt cttttgatca ggtatgcgtg
     2581 gtcaaagcta ttttgaagtt ttaggttgat taaatcatta taaaaacaaa aggctgcccg
     2641 atcggacagc ctttcttttt tctctatatt aagatttaag cgttgatctg tgactcgagg
     2701 taagacacag catcccctac agtttgaatt tgctcagcct gctcgtcagg aatggagata
     2761 ttaaattcct tttccagctc cataattaac tcaactgtat ctaaagaatc tgcgccaaga
     2821 tcgttggtaa agcttgcttc cagagttact tcagactcat caacaccaag tctttcaaca
     2881 atgatttttc tgactttttc ttcaatactt tgtgacattt tacgtttgtt taagttagat
     2941 ttattaaata aagatgcaaa gtaaatcatt cctacacctt taaacaaaga tttgaggcat
     3001 tattttttca ggagagaatg attttccttt ttttcataca tatataggac gtcggcaatt
     3061 ctttgggtca ctttttccaa caatattttt ttagattttt cgccggctgt tctcaaatct
     3121 tttcttcaac actcatcact caattgaatt tttagattat aacacatcat tacccgactt
     3181 ccggcacttc ttcttctcct tcttcttcag cccggttata gtatttgaaa actatattgg
     3241 ctgccctttt cccggtgaca ccagccagtt cttccggaga ggcagctgca attcgcttga
     3301 cgcttttgaa gtgttgcaac agtttttcag ctgtggcctt tccgatgcca tcgatttggg
     3361 tcaattcaga tactgtaaaa ttcatcgacc ttttcttgcg gtggaaagtt atggcaaacc
     3421 gatgggcctc attcctcaga tgctggatga gcttgagcga ttcggatttc ttattgatgt
     3481 acaatggcag ggaatcaccc ggaaaatata tttcttccag cctcttcgct attccgatca
     3541 gcgtaacctt acctaagatg cctaatttgg taagtacctc cgcagccacc cccaactggc
     3601 ctttgccacc atcgatgatg atgagatcag gcaatggttg cccttcttca agaagccttg
     3661 tatatcttcg ggtcactact tcctccatcg tcgcgaagtc attggggcca tcgactgtct
     3721 taacattgaa cttgcggtat tcactcttgg caggtttggc atttttaaat accacgcagc
     3781 tggagacagg attggtaccc tgaatattac tgttatcgaa gcattcaatg tgattgggca
     3841 gcctgtccat attgagatcc ttctgaaggg tctccataat tcgctgagtg ggagtaacac
     3901 ggggcgcatt gatggattcc tttcgtttct gaagcaagta gaaatggatg ttcttctcgg
     3961 ccatctccat cagtttgcgc ttatcgccta tgcttggtat ctgcactttc agctcttttt
     4021 catcgagaat cacaccttcc atattgacca agacctccgg agcgatactg ttaaatcttt
     4081 cccttagctg ccggatacca taggaaaaat tgaatgcgtc atcatccaga ttacgaatac
     4141 cttcctggag aaatgtattg atcatcatgc cgttgaccac tttgatataa ttgatataaa
     4201 actcattttc ctcggcatga taggcaaaga catctacgtc cctgatggtt gtactgacta
     4261 ccatggactt agcctgatag tcttcaaaca gcgatagttt gtttttcatc tcagcagcac
     4321 gctcaaattc caggttttca gcaaatgcat ccatctgttc cctcagatac gcctttaccg
     4381 gtgcaaaatt gccgcgcatg atgtttttaa tctggtcaat cttggcattg tatgcctctt
     4441 cactttccaa tccttcacaa ggtcccatac agtttttcaa atgatattcc agacaaacct
     4501 tgaattttcc tttcctgata ttttcgggag tcaggttgag attgcatgta cgcagtggaa
     4561 acaggttctt gatcaggtcc agaatctgct gtaccctaaa tttggaagta aatggcccga
     4621 aataggtcga accgtcctta atcacctttc gggtaaagaa aaccctcgga aaaggctctt
     4681 tcctgataca tatataggcg tagctcttgc catccttgag catgacattg taccgtggtt
     4741 gaaacttctt gataaacgtg gcctccagca gcaatgcatc gtgctccgtc tcgacaaccg
     4801 tatattccag atggtccgca tgatctacca tgatccgcgt ctttccggct ttattggtgg
     4861 aattgaaata ggaggtaagt ctgtttttca gatttttagc cttgccgaca taaatgatgt
     4921 tgtcatcctt gtccataaac ctgtagactc cgggttctct cggaatagaa ggagatattt
     4981 ccctgaatgc ttctttcttc atccttgtca aacaaattta gagccactca gttgaatcgc
     5041 ctgaattttc caatgcagtc aggagcatga attgattgac tgcgcggtta aaattgtgac
     5101 ccggataagc cgcaccgtaa tagacatcat tgtgcaggtg atccgtcatg aatcgcaatg
     5161 cctgcatgta tatcaatatc attcctgaaa tcctcaggga ttttttttcg ggctcagtca
     5221 tgacatcgcc gagttcctcg agataggcat tttcaatagc ctcaagatat tcctttctta
     5281 tcgtgatcct gtccaactcg gtttcctctt ctgacaccgg acatacatag gtacgcacca
     5341 tatctcccca gtcgctgaaa atgcgtccgg ccatcacggt gtccagatcg atgacgcata
     5401 tacccttgtc atttctgtcg aaaagcacat tgctgatctt ggcatcgtgg tgcataacgc
     5461 gcactctgaa ctgttcggat gcaaccatcc gatcgtggat atctaccaat cgattcttct
     5521 gaagcaattg atcgataatt tccttcgaag attcgattct ttccggattg cctgacttaa
     5581 gtgcatgatg gaattgatca aaacgcaacc ttagattgtg aaaatccgga aggacgggct
     5641 tcaataagcc atgatcaaat ccatggaatg ctgccgtaaa cctcccaaac tgcctagctg
     5701 cttcgtatgc ctgttccgga gaattgacga ctgacacagt cacactgtcc tccacccaag
     5761 gaaagattct gtaataactt tctccgtcaa aaaaaaggga acggtcatcc tgatcagaca
     5821 aaggcaggac caggtggacg tccttaacag tccttgccaa ataagttctc agtaggtcta
     5881 aattaccatg aatcaattca ggatcagtaa aaacatcctt gttgacctgt tgaaggacga
     5941 aggaagaaga gtcaaaggta agtttccatg tatgattaat caagcctgac ttgataggta
     6001 ataaagtgaa actgcctgtt tccggaaaga aatgttcgat tgcacgctgg atggattctg
     6061 acataaatta cagtctgttg tcaaaataaa aataatacaa ttatcgaaag ttccggatta
     6121 aaaaataaaa agcccttttc aattactctt gtctttgaca ccgctacccg gatacgccgg
     6181 ctttattaaa tttataattc ggttccccag aagtcactta acagggtcga ttttgtaacc
     6241 gggaaatcaa agtaagaaaa acaatgtgtc aggcccgtcc ttttgtaatc actccgatga
     6301 caccactacc aaagattaaa taaaattata atatatatcg atatattttg taccttgcaa
     6361 gcgcattgcg aggcagttat gtattgcaac aaaagggttg aatcatgatc caacacttca
     6421 ctaaacgaaa agctggggca tgacgatgat tcaataccac cgcatttgag gtttcagaaa
     6481 aaagaaccat gcataagcag gtcaatgggc ttttactaac aatttgatgt acagatccat
     6541 gtctggcaaa ggaagacaag ctacaatctt attaagtaca gcgtttttat tgctggcatc
     6601 aatcggcatt tggacatatg tttctcaaaa gaatatcgaa aagcggcaaa ttgctgatat
     6661 tgctctccgg ttccaaaaac tagaaagaaa ggcacaagaa agcattgata acaaggagat
     6721 aatcgacttc ctgagccaat ggtcagaggg cacctaccta acggataagg catctgaaaa
     6781 actgattacc aagctggcca cttcgcctgt tgtactcggc atggaggccg gtggcagact
     6841 caccgcatgg acaggcaaca ttcatagtca agaaaccgga aagtccctac tcaatgggta
     6901 ttacctgatt gatgattcca tattccaaaa gaaatacccg atcgccacag gacaggatat
     6961 caaggtagtt gttgagtttg ggttaccggg aaagttttac aaagagaaga aggatgggct
     7021 tacagaatta aaatcgggtg atggcaacct gaccgcctat tttttgccat ctcccaaaca
     7081 aaaaagtctc agtggtgact ggaagatact gcctttttat cttgcagcct ataccctgtt
     7141 gctgatcggt ctatccaaga gactgaaaag gaaaacgaaa gtaccattta tctggtcaat
     7201 cggatctact cttttgatag ccattgctta caggttgctg gattggtttt taggagcagg
     7261 catgtccata gatataggac tgatcaactc gggtctcaca cccaaaccca tactgagcga
     7321 atggtttgcc aattggatac tgattctatt gatctacttc ctggtcattg aaaacgtcat
     7381 tgacaatttg ccacccatca aagcaagcaa aaggaaaaga aatcccctcc ttctgtacac
     7441 taccggactt tacgcactcg actatctagg ctttgtaggc atattggtct tttgccggga
     7501 attggttttg cgcagcggaa tcaattttga cttcagtaat gtgctaaatc tgggtgttgg
     7561 gcctttgata agtcttgtat tgctggcttt gttgctattc aggtatttcg ttttcgccta
     7621 taagatcaac cttgtcctgt ataaaaacag gatcaaactc aacaacaagc tcattggcat
     7681 gataggcggc tttctgctca tgcttccctg gctgctttta ctccgtctgg atttgcctct
     7741 ttttatcctc atattggtag tattgctttt tgccttacta ttcgatatct tcatcagcac
     7801 accctcgcca tcattgggct ggctggtcat ctggatattc ttcttttcgt cctttgccac
     7861 cattgccctc ttcaaattca atcgggacaa ggatcatgat gacaggctcg cgctgatcaa
     7921 cattttaaaa aataaggagg atagcttatt attacgtcag ttcggagaat ttgtcaacaa
     7981 cttgaaaaat gacaggcaga acaatgctgc tatgactgaa gcgttgctgc aaggcagctc
     8041 catttcatcc agcaatgtta tccagaatgt gttggatgaa tttgaatata tccgccaatt
     8101 ttacaatgtt gagttttttg ttgcacaaaa tgattctcta ttacaggagt ccataccggg
     8161 cttgaaaaac aattttgtaa gtcagggagt caaattcagt gaagccggac cttaccttat
     8221 agccggccgg agaaagacct ttcatggata ctggctgaag atctattggc aagggtatcc
     8281 ccagcgacca tcacctacta taacattttc cgttactaag aagctgtat
//
LOCUS       DAOQMC010000093         8321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3443_length_8321_cov_4.598234, whole genome shotgun sequence.
ACCESSION   DAOQMC010000093 DAOQMC010000000
VERSION     DAOQMC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3443_length_8321_cov_4.598234"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1047)
                     /locus_tag="PKV31_05105"
     CDS             complement(157..1047)
                     /locus_tag="PKV31_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB92269.1"
                     /translation="MTGFDFPYSPMESVDENINRVSIYLKRDDLVHPVIEGNKWRKLK
                     YNLEFSLQNGIRTIISVGGAFSNHLLALSQAGKLYNLNTRGYLNGENVDLNNPTLSVC
                     RANGMEVILIPKVQFGYYKTGEFLCRSDEYFVPEGGSNEFALKGCADLYYEIEHQLPD
                     VTHIVLPFGSGGTTAGIFRVKRADVEILSVPVLRLNPIEHLGRTYHSDFAGLQYSLDY
                     HFGGYARFNDKLIQFIRDWYARTGIKIDPIYNAKGLYALYDLAGKGYFPEGSKVVYVH
                     TGGSQGIKGFNRRFGLSLPE"
     gene            complement(1044..3353)
                     /locus_tag="PKV31_05110"
     CDS             complement(1044..3353)
                     /locus_tag="PKV31_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNB92270.1"
                     /translation="MNFKIFLLIPWMVIYGFFAFAQSPGKLNIIPQPVKVHELKGSLN
                     LRNYPAYLYGPGIEKDPMFDFNGWASKEFGLEMKRVMQPGNAIKFVKVDTFSHPDAYL
                     LTIDDNGVLIQFKDEAGAFYATQTLKQLCPVESSDIGTNSGVPMSVPYCTIYDYPRFS
                     YRGMHLDESRHFFGKDAVKKYIDMLAFQKYNYFHWHLTDDQGWRIEIKRYPKLTEISS
                     CRDETLIGHYSDKPVRYDGNKYCFYYTQEEIKEIVVYAARKYITIVPEIEMPGHASAA
                     LAAYPELGCTEKQIRPATTWGIFDDIFCPKEGTFFFLQNVLDEVMELFPSKYIHIGGD
                     EVPKAQWENCDYCQKLIRNFRLKDEHGLQSYFIQRIEKYVNSKGRTIIGWDEILEGGL
                     APNAVVMSWRGMEGGFEAAGQDHDVIMTPGAYCYFDYYQARGPEEPVAIGGLVTLKKV
                     YSFEPIPDKLPSSKHKYILGGQANIWTEYIATPKHLQYMAYPRSFALSEVLWSPKMER
                     NYEEFVQRLTTQLPRLDAWDINYARHFYNIYINTKHIDGVERIVLSCDNPDAKIEMRV
                     SNGKTVTAWQEYSAPVPFPKGNGAVEARVQIKGSKSEMPATVKEYETHSAMGAKVKLV
                     NPAHEKYNAGGSEALVNGFKGPENDYGGDEWLGFSGKDIIAILDMESSRTIDRVEARF
                     FVSQGQWIYPPRDMKVYTIDAMGKEIEVIAKSTITENGKIHKIVLNFEKPIETDKLKI
                     KVNRYGLIPAGAQGAGNEAWLFCDEVFVR"
     gene            complement(3583..6033)
                     /locus_tag="PKV31_05115"
     CDS             complement(3583..6033)
                     /locus_tag="PKV31_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNB92271.1"
                     /translation="MFQSLCFKRMAIAWIAMWLMINLNAQKTVVIVRDYAGKGVEDAS
                     VSVNGYGAITDHEGRVEGEFRFPLHVVVTYVGTNGLDTIISEQTSKVELILLGTGILL
                     KEGLVTASWANESSAVTQKTLSKSRIYSDNPADMPSILESLPGSVSTSDAGNGIGYSA
                     LRIRGSDQTRINVTIDGVPVNDAESQDIFWVDLPDIIEDVESVQIQRGVGVSTAGPGA
                     FGANINLRTGMLSKIPLLTANVGYGSFNSQKYGLALSSGVLGKYFRFKIRGSRISSDG
                     YMDRASSQLWAGSFQSQYVRQRFSLAANVYWGKEETYQAWEGTPIQFYKADPGTTFNP
                     AGLKSDGTYYSDQKDFYRQVYSRLIGRWHIKQNSGLQFTLYNTLGKGYYNQYREENIS
                     KYFDDVPSMDVALIRERWLDNVLNGLNLVFDHQNNRWFLQTGGNLQLYRGKHFGIIKN
                     YQGLPDWVPRYYYDNIGEKQEASVFARIEKTLDRIGAFVDLQGRRIAYTYEGLDNEWN
                     PEAITQRFLFFNPKVGATIFLNENKDRNIYIFGGVANKEPNRRDFTDAAPGKLPKPER
                     MYNGEMGYRHSKQNFDLQSNIYFMFYQDQLVLTGKINDVGAYTRFNVDKSYRMGWETD
                     LRWQPFQQLTILGNISLSKSKIIDFTEYSDVSILTDEGVEYPGQVATFKGNTDIAFSP
                     FVVAYGKMSYQPFMQSKQLKGLSFHYTYKFVGSQYIDNTSDIHSKLPSYNLHGMGFSL
                     PLRRGKNGFVLSGNIDNLLNIHYVNNGWIYRFKAIGYDASKDDPYIQSEGNDYYSSVG
                     YFPQAGRRVFVSLRYMLK"
     regulatory      complement(6079..6171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6332..6883
                     /gene="hpt"
                     /locus_tag="PKV31_05120"
     CDS             6332..6883
                     /gene="hpt"
                     /locus_tag="PKV31_05120"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNB92272.1"
                     /translation="MESIKIHDKSFVPFILKDDIDNRIAGIASELENKFKNKQPVFLI
                     VLNGASFFGVDLLKKYRQPCSIQFLRLKSYEGTDSTGNVKSIGNMPENLKGRDIVILE
                     DIVDTGLTMNFLKYKLEEEGVGSITIVTLLDKPSRRVQDVKVEVVGFTIPDVFVVGYG
                     LDYDDLGRNTEHIYKLSDNIKSE"
     gene            complement(6947..7438)
                     /locus_tag="PKV31_05125"
     CDS             complement(6947..7438)
                     /locus_tag="PKV31_05125"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNB92273.1"
                     /translation="MIQVFVTGGTFDKEYNFITGELYFKDTHLNTMFVRGRSSLEIDV
                     KTLMMVDSLEMTEEDRNIIVYQCKKSPAEKILITHGTDRMTATARAIAEAEIEGKTIV
                     LTGAMVPYAFGNSSDGFFNLGAALAFVQVLPPGVYIAMNGKYFPWNNVRKNYDTGFFE
                     EIE"
     gene            complement(7486..>8321)
                     /locus_tag="PKV31_05130"
     CDS             complement(7486..>8321)
                     /locus_tag="PKV31_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNB92274.1"
                     /translation="PELEDIPFLPITFFKNFRITSCDWQEQTVFRSSSTTGKGRSSHY
                     IRDLQIYKTVSLKIFEKYFGPISHLRIMALLPGYLEREDSSLVYMVNYLMGCTAVDQR
                     YFFLDRYHDLMEVVQGNLDEHIILFGVPYAFIRCFRELGYQFWPNVTMIETGGMKGKE
                     AEMTKAELHLFLEESFRPKSIYSEYGMTELLSQCYSKGEGKFEVSDTMYMIVKELNDP
                     FSIQKRGKPGVLNVFDMANVHTCSFIETEDMAILHENGEFEILGRVDNREIRGCNLLA
                     G"
BASE COUNT         2499 a         1945 c         1362 g         2515 t
ORIGIN      
        1 cccggaatga attatcaaca cttgattcaa cgctattaaa catctacata cttcagggcg
       61 gccacccaat tctcccgctt tgcgctacct cctcatttaa atgcatcttt ccaattagaa
      121 tgcctccgct ttaagatttc ttagacccaa atcctatcac tccggcaaac tcaatccaaa
      181 tcgccgatta aatcctttta tcccttgact accaccggta tgcacataaa caactttaga
      241 accctcggga aaatagccct ttccggccaa atcatataat gcatataacc ctttggcatt
      301 gtaaatggga tctattttaa tgcctgtcct tgcataccaa tctctgatga attggatcaa
      361 tttgtcatta aatctggcat aaccgccgaa atgataatcc aggctgtatt gtaacccggc
      421 aaaatcggaa tgataagtcc tccctagatg ctcaattgga ttaagcctca gaaccggaac
      481 tgataagatc tcaacgtcag ctcgtttcac tctgaaaata cctgcagtcg ttccaccact
      541 tccaaatggc aacacgatat gcgtcacatc aggcaattga tgttctattt cataataaag
      601 gtctgcgcaa cctttcaaag caaactcatt actaccacct tcaggaacaa aatattcatc
      661 agatctgcaa agaaattcac cagttttgta atatccaaat tgaaccttgg ggatgagaat
      721 cacttccatc ccatttgcac gacacacact taatgttgga ttattcaagt ctacattttc
      781 tccatttagg tagccgcgtg tattcaaatt atataatttt cctgcctggc taagtgcaag
      841 aaggtgattg gaaaaagcgc ctcccacact tataatggtg cgaattccat tctgaagaga
      901 aaactcaaga ttatacttca acttccgcca tttattccct tcaatgaccg gatgcacgag
      961 atcatcgcgc ttgaggtata tacttactct atttatgttt tcatctacac tttccattgg
     1021 actatacgga aagtcaaatc ccgtcatctg acgaatactt catcacagaa aagccaggct
     1081 tcattaccgg caccctgagc accagcgggt attaagccat acctattgac ctttatcttc
     1141 aatttatctg tttcaattgg cttctcaaaa tttaatacaa tcttatgtat tttcccgttt
     1201 tctgtaatgg tggatttagc aatcacctct atttccttac ccatcgcatc gatggtatat
     1261 accttcatat ctcgtggtgg gtaaatccac tgtccctgac taacaaagaa acgagcttct
     1321 accctatcaa ttgtacgaga agattccata tcaaggatag caatgatgtc cttacctgaa
     1381 aatcccaacc attcatcacc accataatca ttttcaggac ctttgaaccc atttacaagt
     1441 gcttcgcttc ctccggcatt gtatttttca tgggcaggat tgactagttt tactttagcg
     1501 cccattgcgg aatgtgtttc atactccttt acagtggctg gcatttccga tttgcttcct
     1561 ttgatctgca cccgagcttc aacagcacca ttaccctttg ggaaaggcac cggtgctgaa
     1621 tactcctgcc atgcagtcac cgtcttacca ttggagacac gcatttcaat ctttgcatca
     1681 ggattgtcac aagacagcac aattctttca actccatcaa tatgtttcgt attaatgtaa
     1741 atgttataaa aatgccttgc ataattaatg tcccaggcat cgagtcgagg caattgggta
     1801 gtcagccttt ggacaaactc ttcataattc ctttccattt taggagacca tagcacttct
     1861 gacaaagcaa atgagcgtgg gtaagccata tattgcaaat gtttcggtgt tgcaatatac
     1921 tctgtccata tatttgcctg tccccccaat atgtatttgt gcttcgagga aggtagctta
     1981 tccggaattg gctcaaatga atatactttc ttcaaagtta ccaaaccacc gatagcaacc
     2041 ggttcctccg gaccccgggc ctgataatag tcaaagtaac agtatgcccc tggagtcatt
     2101 ataacatcat gatcctgacc agccgcctca aatccacctt ccatcccccg ccatgacata
     2161 actactgcat ttggagcaag ccctccctca aggatttcgt cccaaccaat aatcgtccgg
     2221 cctttgctat ttacatattt ttcaattcgc tgaataaaat aggactgtag gccatgctca
     2281 tctttaagcc ggaaattcct tatcaatttc tgacaataat cacaattttc ccactgagcc
     2341 ttaggaactt catcaccgcc gatatggata tattttgatg gaaataactc catcacttca
     2401 tccaggacgt tttgcaaaaa gaaaaatgta ccttcttttg ggcagaagat atcatcgaaa
     2461 ataccccaag tggtggcagg tcttatttgc ttttctgtgc aacccagttc aggatatgca
     2521 gccagtgcag ctgatgcgtg gccgggcatt tcaatctcag gtacaatcgt aatatatttc
     2581 ctggctgcat atactacgat ctcttttatt tcttcctgtg tataataaaa acaatattta
     2641 ttcccatcat agcggacggg tttatcgctg taatgcccga ttaaagtttc atcacggcaa
     2701 gaagaaattt ccgtcaattt gggatatctt tttatttcta tcctccagcc ctggtcatcc
     2761 gttaaatgcc agtgaaaata attatatttc tggaacgcaa gcatatctat gtactttttg
     2821 acggcatcct ttccgaaaaa atgacggctc tcgtcaaggt gcattcctct ataggaaaat
     2881 ctggggtaat catatatggt acaatatggt accgacatgg ggacaccgct atttgtacca
     2941 atatcagaag attctaccgg acatagttgc ttcaatgttt gtgtagcgta aaacgccccc
     3001 gcttcatctt taaactgaat aaggactccg ttatcatcta tcgtgagcag atatgcatca
     3061 ggatggctga acgtatctac cttgacaaat tttatggcat tgccgggctg catcacacgc
     3121 ttcatctcta aaccaaattc tttacttgcc catccattga aatcaaacat cggatccttc
     3181 tcaatgccag gaccatatag gtatgccggg taattacgaa gattaagact tccttttaat
     3241 tcatgcacct tgacaggttg agggataata ttaagttttc caggactttg cgcaaaagcg
     3301 aagaatccgt atattaccat ccatggaatt agtaagaaaa ttttgaaatt cacggcaaat
     3361 gtctgttttt atttatcaat caaattatta ctcacaaggt taatagtcgt aaagatatgt
     3421 ttatttcggg aggaaaatac acagttactt catttggtat aatatttacc atatcatttt
     3481 gataataata ttattaaata accgcatata acttaacacc aaaaatacat cttgttatcc
     3541 tacatatatt caatatttta ggaataataa ttcccgggaa aatcacttca gcatatatct
     3601 caaactcaca aatacccttc tgccggcttg aggaaaatac cctacagaac tataataatc
     3661 atttccttct gattgaatat aaggatcatc tttactggca tcataaccaa ttgccttaaa
     3721 tcggtaaatc cacccgttat tgacataatg aatgtttagc aaattatcaa tgtttcctga
     3781 caatacaaat ccatttttac ctcttcttaa aggtaatgaa aagcccattc catgaaggtt
     3841 gtatgatggc aatttactat gaatgtctga agtattatcg atatactgac ttcccacaaa
     3901 tttgtaagta taatggaatg acaatccttt caactgcttt gattgcataa aaggttgata
     3961 ggacattttt ccataagcaa caacaaaggg actgaacgct atatccgtat tccccttaaa
     4021 tgtggcaacc tgtccgggat attcaacacc ctcatccgtt aaaatactca catcgctata
     4081 ctcggtgaag tcgataatct tgcttttact caaggagata ttccccaaaa ttgtaagctg
     4141 ctgaaacggc tgccaacgca gatcagtctc ccatcccatc ctataacttt tatctacatt
     4201 gaatcgtgtg tatgcaccaa catcattaat cttgccggta agtaccaact gatcctggta
     4261 gaacataaaa taaatattgg attgcaaatc aaagttttgt tttgaatgcc tgtatcccat
     4321 ctcaccatta tacattctct ccggtttggg caatttgcct ggtgcggcat ctgtaaagtc
     4381 cctacgatta ggctctttat ttgcaacgcc tccaaaaatg taaatattcc tgtctttgtt
     4441 ttcattaagg aaaatggttg caccgacctt tggattaaaa aagaggaatc gctgggttat
     4501 agcctccgga ttccactcat tatcaagtcc ttcataagta taagcaattc gccgaccttg
     4561 caaatcgaca aaagctccta tgcggtcaag tgttttttca atcctggcaa agacactggc
     4621 ttcctgcttc tctcctatgt tatcataata atatctaggg acccaatcag gcaagccttg
     4681 ataatttttt atgatgccaa aatgctttcc gcgatacaac tgaaggtttc caccggtctg
     4741 tagaaaccat ctattattct gatgatcaaa caccagattc aacccattga gcacattatc
     4801 aagccatctc tcccttatca aagccacgtc catggaaggg acatcatcaa aatacttaga
     4861 aatattctct tcccgatact ggttataata acctttaccc agcgtattgt ataatgtaaa
     4921 ttgcagcccg ctattttgtt tgatatgcca tcgaccaatc aacctgctgt acacttgccg
     4981 atagaaatcc ttctggtcac tataatatgt cccatcactt ttcagaccgg ccggattaaa
     5041 tgtcgtccct ggatctgcct tataaaactg aatcggcgtt ccctcccatg cctgatatgt
     5101 ttcctccttg ccccaataca cattagctgc aagactgaat ctttgacgca catactgaga
     5161 ctgaaacgaa ccagcccaca attgactcga tgccctgtcc atatatccgt cagatgaaat
     5221 ccggctaccc cgtatcttga atctgaaata cttccccaaa acacctgagc tgagggccaa
     5281 tccatatttt tgactattga atgaaccata acccacattg gcagttaaaa gtggtatctt
     5341 actcagcata cctgtccgga gattaatgtt ggctccaaaa gcaccgggac ctgcagttga
     5401 cacacctaca cccctctgta tctgaacact ttcgacatcc tcaattatgt caggcaggtc
     5461 cacccagaat atatcctgac tctcagcatc attgaccggc acaccatcaa tagtaacatt
     5521 tattcgtgtc tgatcgcttc ctcttattct caatgccgaa tacccaattc catttccagc
     5581 atctgaagtt gaaacactcc ctggtaagga ttctaatatg gacggcatat cagcaggatt
     5641 atctgaataa atgcggcttt tagaaagcgt cttttgtgtt acagccgaag actcattggc
     5701 ccaggaagct gtaaccagac cctcttttaa aagaattcct gtacccaata atatcaattc
     5761 tacctttgat gtttgttccg atattattgt atccagtcca ttagtaccaa catatgtaac
     5821 tacaacatgt aatggaaagc gaaattcacc ttccaccctt ccttcatggt ctgtaatggc
     5881 tccatagccg ttgacagaaa ccgatgcatc ttcaacaccc ttccctgcat aatcccgaac
     5941 aattacaacc gtcttctggg cattcaaatt aatcattaac cacatggcaa tccaggcaat
     6001 cgccattcgt ttaaaacaca aagattgaaa catttttatt taatttatga atgctggaac
     6061 atggggttaa tcccaacttc tatccacaaa ttaccatccc ttccactatt accatggcag
     6121 gttcaatggg ttttatctca gccggttaca ccagcacccc agttaataag atgatgcaaa
     6181 tttaccgtac ttttacttat ttacaaattg ataaaacaac attttctaag tatctgaatg
     6241 aaatttgtat ttgtatcttt gccgggattg attcagttga tctcatcagg gagctttatt
     6301 cattgatcac aaacaattta aaatactaac attggagtct attaaaattc acgacaaatc
     6361 ttttgttccc tttatcctca aagatgatat cgacaacagg attgccggga ttgcttcaga
     6421 attggaaaat aaatttaaga acaagcaacc ggtttttctg attgtattga atggcgcctc
     6481 cttttttgga gttgatttat tgaaaaaata cagacaacca tgctccattc agtttctccg
     6541 cttaaaaagc tatgaaggaa ctgattcgac aggtaatgtc aaatcaatag ggaacatgcc
     6601 ggaaaattta aaggggcgtg atatcgtcat cttagaggat attgtggata caggcttgac
     6661 catgaatttt cttaaatata aacttgaaga ggaaggtgtt ggttctatta ccatcgtgac
     6721 actattggac aaaccgtcca ggagagtgca agatgtaaag gttgaagtcg taggctttac
     6781 cataccggat gtatttgtcg tgggttatgg tctcgattat gatgacctcg gaaggaatac
     6841 tgagcatatc tacaaattgt cagataatat caaaagtgaa taatccgcct aataatttcc
     6901 ggaaaatttt aaaattaatg gaaaataggc accccatccg ataatattat tcaatttcct
     6961 caaaaaaacc tgtgtcataa ttcttacgga cattgttcca tggaaagtat ttaccattca
     7021 tcgcaatata aacacccggc ggtaagacct gaacaaaggc aagtgcagct ccgagattaa
     7081 agaaaccatc actggaatta ccgaatgcat aaggcaccat cgctcccgtc aggactatcg
     7141 tcttaccttc aatttcagct tcagcaatag ctcttgccgt tgcagtcatc ctatccgtcc
     7201 catgtgtaat aagtattttc tcagcgggtg attttttaca ctgatatact atgatattcc
     7261 ggtcttcttc cgtcatttca agactatcca ccatcatcaa tgtctttaca tcgatttcca
     7321 gactgcttcg gcccctaaca aacatagtat taagatgggt atccttgaaa taaagctcac
     7381 ctgtaataaa attatactcc ttgtcaaaag tccctcctgt aacaaaaacc tgaatcatta
     7441 ttgaaatatt taatgcaaac atattaaaaa aaaacggtaa aagccctatc ctgcaagaag
     7501 attacagccc cttatctccc gattatctac ccgacccagg atttcaaatt caccgttttc
     7561 atgaaggatg gccatatcct ccgtttcgat aaaagaacag gtatgcacat tagccatatc
     7621 aaagacattc aaaacacctg gcttacccct tttttgaatt gaaaaagggt cattcaactc
     7681 cttcactatc atatacatag tatcactcac ttcaaatttc ccttcgcctt tcgaataaca
     7741 ttgggaaagt aattccgtca ttccatactc cgaataaatt gattttgggc gaaaggattc
     7801 ctcgagaaat aaatgcaact cggcttttgt catctcagct tctttgcctt tcatcccccc
     7861 ggtttcgatc atggtcacat tcggccaaaa ttgatagcct aattccctaa agcaccgaat
     7921 aaaagcatag ggaacgccga aaagaataat atgctcatct aaattccctt gtaccacttc
     7981 cattaaatca tgatatcggt caagaaaaaa atacctttga tccacagccg tgcatcccat
     8041 taaataattt accatataga ccagactact atcctctcgc tcaagatacc cgggtaaaag
     8101 tgccataata cgcagatggc tgattggccc aaaatacttt tcaaatattt tcagactgac
     8161 tgttttatat atttgcaaat ctctaatata atgactactt ctcccctttc cggttgtaga
     8221 gctggatctg aacactgttt gctcctgcca atcacaggat gttatacgga aattcttgaa
     8281 aaatgtaatg ggcaaaaaag gaatgtcttc caattcggga t
//
LOCUS       DAOQMC010000094         8238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3492_length_8238_cov_5.180496, whole genome shotgun sequence.
ACCESSION   DAOQMC010000094 DAOQMC010000000
VERSION     DAOQMC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3492_length_8238_cov_5.180496"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..2587)
                     /locus_tag="PKV31_05135"
     CDS             complement(419..2587)
                     /locus_tag="PKV31_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB92275.1"
                     /translation="MAEYFERLMHGFELPLKSPVLVFSLILLIILLSPILMRKLNIPG
                     IIGLIISGVIVGPHGLKLLEKNSAIELFSTIGLLYIMFIAGLELDLNEFKANKNKSLL
                     FGFFTFSIPLAIGFPVVYYFLDYNFNASFLTASMFATHTLVAYPIVSRLGVSKNQAVA
                     ITVGGTILTDTAVLIILAVVMENSKGSLNQEFWIRLAISLAIFSVIMFLLIPRIAKWF
                     FRKLESEKHSHYIFVLSVVFFAAFLAELAGIEAIIGAFVAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDISVILSGPQALIIAATLSVVAISGKWIAALFTQLAFR
                     YSGAQRQLIFGLSSAHAAATLAVILVGFNAGVLDENILNGTVILILITCIIASFATEK
                     ASKKIILDSGNDSLDLDSSSNTNDEHILLPIANVEKMDQLLDFAIFLKDAKSVNPISI
                     LTVVSNNDEAEINIKKARSKLEDFVKQASASETNISIITTIDHNPGSGIARISKEIMS
                     DIIVLGWPQKTGLIDKLIGEKVANIMNSTDKCAFICHIDKPTNTHKRIIVAAPPLAEH
                     EKGFEMWMRKVSKLAQEFSIPVHIYCNQNTGKMVEKCSKKIKTSANIQVILFDDWEDF
                     LVLSRNIHQDDLIILVSSRRGAPSYMPVLESLPAKLEKHFKTNSRIIIYPQQYNIEQS
                     LEGFEDVNAETLNKGLETVQRIGRKIGGIFRKEESKVTSE"
     gene            complement(2802..3440)
                     /gene="recR"
                     /locus_tag="PKV31_05140"
     CDS             complement(2802..3440)
                     /gene="recR"
                     /locus_tag="PKV31_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNB92276.1"
                     /translation="MKYSSQLIGKAVESFSSLPGIGKKTALRLSLHLLSKDAMWVQAF
                     SESFIKLRNDLKECDECHNLGDEVLCSICTDKRRDNGILCIVETIRDLMAIEETGHFQ
                     GFYHVVGGLISPIDGIGPDQLNIEDIEQRIINKNIREVVMAISPNIEGETTMFYIAKR
                     LQHLPIRISTISRGISFGGELEYADEVTLGRSIMARIPYMQFEPSTAEHGDR"
     gene            3810..6758
                     /locus_tag="PKV31_05145"
     CDS             3810..6758
                     /locus_tag="PKV31_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92277.1"
                     /translation="MTRYVLKQGLVLLLLFLFGNLHGQISGTVRDASGQGLVGVTILA
                     IGENNGTVTDIDGNYSLRVNTANALEFSYVGYQTQKINIDGRKMIDVVMQEDVKALKE
                     VVVVGYGVEQKKTLTSSVGIVNSDAIKDLPVPTIDGVLQGQASGVQVVQNSGTPGGGM
                     SVRIRGVSSISGSSQPLYVIDGIPVTTGDFAQVGYEGQGVNQLSDLNPGDIESITILK
                     DAAAATIYGARASNGVVLITTKRGSSKKSTINLSASYGWQQPWRKLEMLNAHDWKLYR
                     NDYFGTEVYTPEQINDNTIDTKWQDYIFRTAPISNYELSTSGGDEKTRFYISGNLFNQ
                     TGTLIGSDYRRMNGRVNLDHKINNSVTIGTSIGLSYAKTNRIEGDQSLHGVLPNGIST
                     PAVFPVYNSDGSYNQDGPYSNPVSIANEATNENFSYRGLGNIYLDWKIWKGLTFSTKW
                     GIDFMNFREHAFEYNTVQGRKYNGLGFETYTNVGNLVSNNTLKYSFDLQDNRFEVLAG
                     YSFERKNVASSYIRGQDYADASLQYINSASTITSASSGASNEGIRSLFGRINYNLKDK
                     YLVSLTGRMDGSSRFSENNRNGFFPAVSAAWRLGEEDFFKSNVISELKVRGSFGLTGN
                     DDIPQFLYAELYGNTTYGGKPAVYPSNIPNPDLKWETTTQVNAGLDIGLFKDRIYLNI
                     DLYRKFTKDLLLSRPLPLSSGFSTITENIGEVENKGIELGLTTENLADRKLEWNTRIN
                     FSLNRNKITKLYNNQPIDDLGRGSNRIEVGQPIGIFYSYEWLGVDPSTGDVVFADKNF
                     DGNITSDDRTIIGNPHPDFIYAITNNFKYGRFDLSIFFQGSQGNDVMNGSRLYLESLQ
                     GGDNQLAAINRRWKNVGDITDIPRATNDPVAAASNKRLSSRFLEDGSYLRLKNLTFGY
                     NFNTKNIMKGKISSLRIYFTGQNLLTFTNYTGLDPEVNYVGDDNARMGTDFFTYPQAR
                     SFSFGLNAGF"
     gene            6760..8088
                     /locus_tag="PKV31_05150"
     CDS             6760..8088
                     /locus_tag="PKV31_05150"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNB92278.1"
                     /translation="MKRILILLVLVYSFSSCNVLDVEPTNSIPASEAFKTKADLNRGI
                     LGAYNSLQGLSYYGRAYLTVSDLSADNLLYPPSATTADYLDIDNNHILPENGVIEGIW
                     GVAYDGLNVANNVIAKIPEMSFLNQDEKNVALAELYFLRALNHFNLLNYFGGIPIKTK
                     PTIGLSEINAARNSPEEVYATIIQDLTFASTYLPKSSASKIRASQGAANALLARVYLY
                     KKDYADAVIYASKVIDDNNYSLMSNYSDIFAADESKESIFEIDFTSLDRNRIAEYNFP
                     LTLNGRGSVGPDPGIVSAYEANDKRKDASIAFAGTIPYAIKYHDLATGSDNVIVLRLA
                     EMYLIRAEANARLNGNINDIRKDLNTIRSRAGLEEAVANSIDELLLAIEAERRVELAF
                     EGHRWFDLVRTDRAIDLLPNVTQKYQYLFPIPQSELLANTNPGMKQNPGY"
BASE COUNT         2600 a         1633 c         1584 g         2421 t
ORIGIN      
        1 catcgtcttc gacctgatga tcatcatgca aacactgcgt gtggtgatat ttcctgaccc
       61 ttcatccaca ctgcctttcc aaattgcgtt tgctacatgc tttttcatta tctgattttt
      121 tatgatttaa attatttgag cttaatttga tgcacacaaa tcttatttag aataaatcaa
      181 tataaaattt gttcatgaaa ttagttactt tgaatattta aagacaaaat ctttaccacc
      241 agatataata aaaaacggct aaaagacgat ctacttattt tcaataaatt ttcaacgaaa
      301 gtaacttgaa tacatatttt ttaaatatga caatcctatg ggatagtacc caaggctgga
      361 tccaaagaac tcattcaatc aaacattaaa caaagagcta gaagaaatga cccaacaatt
      421 attccgaggt cactttggac tcttctttcc tgaagattcc accgattttc cttccaattc
      481 tttgcacggt ttcaaggccc ttattcaaag tttcggcatt gacatcttca aaaccttcaa
      541 gactctgttc tatattatat tgctgtgggt aaattatgat cctgctattt gttttgaaat
      601 gcttttccaa tttggcagga agactctcta atacgggcat ataggaagga gctcctcttc
      661 tggaagacac aagaataatc aagtcatcct gatggatatt ccgggacagt accagaaaat
      721 cttcccaatc atcaaacaaa atgacctgaa tattggctga tgtctttatt tttttactgc
      781 atttttcgac cattttaccg gtattttgat tgcaataaat atgaacaggg atactgaatt
      841 cctgggcaag tttggatacc ttgcgcatcc acatctcaaa tcccttttca tgctcagcca
      901 aaggaggagc tgcaacaatt atccttttat gggtattggt tggtttatcg atatggcata
      961 tgaaggcaca tttatctgta ctgttcatga tattggcaac tttttcaccg ataagcttgt
     1021 caatcaatcc tgttttttgc ggccagccca atactataat atcggacatg atttcttttg
     1081 aaatacgtgc gattccgctt cccggattat gatctatcgt agtaataata ctaatattcg
     1141 tttcggatgc cgaagcttgt ttgacaaagt cctccagttt acttctggct tttttaatat
     1201 tgatctccgc ttcatcatta ttggacacga cagtaagtat agaaattggg ttgaccgatt
     1261 tggcatcttt aagaaaaatg gcaaagtcaa gcaattggtc cattttttcc acattagcga
     1321 taggaagtaa aatatgttcg tcattggtgt tgctggatga atccaaatca aggctatcat
     1381 tgcctgaatc cagaataatt tttttggaag ctttctcagt agcaaaagaa gcgatgatac
     1441 aagtgatcaa tataaggatt actgtaccat taagtatatt ttcatctaat actccggcat
     1501 taaaccccac taaaattact gcaagagtag ccgcagcatg agcactgcta agaccaaata
     1561 ttaattggcg ttgagccccg gaatatctga atgctaattg ggtgaataac gctgcaatcc
     1621 atttaccact aatggctact accgatagag ttgcagcaat gatcaacgcc tgtggcccac
     1681 tgagaatcac actgatatcc accaacatac cgacactgat caggaaaaat ggtatgaaaa
     1741 gagaattacc gataaattca attctgttca tcaaggctga agaatggggg atgagtttgt
     1801 tgagtgccaa tcctgctacg aaagctccga ttatggcttc tatcccggcc aattctgcca
     1861 gaaaggcagc aaagaaaaca acagatagta caaagatata atgtgaatgt ttttcacttt
     1921 ccaatttacg aaaaaaccat ttcgctattc ttgggatgag caaaaacatg atcacagaga
     1981 aaattgcaag ggatattgca agcctaatcc aaaactcctg attgagactt cccttactat
     2041 tttccatgac tacagctaaa ataatcaaca cggcggtatc ggtcagaatg gtccccccca
     2101 cagtgattgc aactgcttga ttttttgaaa cccctaaccg gctcactatt ggataggcaa
     2161 ccagggtatg tgtagcaaac atacttgctg taagaaaact tgcattgaaa ttgtaatcaa
     2221 gaaaataata gacaaccgga aatccaatcg caagaggaat agagaaggta aaaaatccaa
     2281 aaagaagact cttgttttta tttgctttaa attcattcaa atccagctca agcccggcaa
     2341 taaacatgat atacaacaaa ccaatggttg agaataattc tatcgctgaa ttcttttcaa
     2401 gaagttttaa tccatgtgga ccaacaataa ctccggaaat aatcaatcct atgatgccgg
     2461 gaatatttaa ttttctcatc aatatcggag aaagaagaat aatcagaagt atcaatgaaa
     2521 aaacaagtac agggcttttc aaaggcaact caaatccatg catcaatctc tcaaaatatt
     2581 ccgccataca tcacaatttt ttaggcagtc tgtcaatatt ataaatatca caatcctgaa
     2641 gaaaaacaat actcaacgca aacttattcg ctttccgaag ccaaattaaa gcgtgtaaat
     2701 tatgtaaatt ttctgtaaat ggtcaattaa gtaccgattt ttttgcccaa taaaaaatta
     2761 aaaagaaggc aatctgcaga tatctaacgg ccaacaattc ttcaacggtc accatgctca
     2821 gcagtagaag gttcaaactg catataaggt atcctggcca tgatcgacct tccgagtgtt
     2881 acttcatcgg cgtattccaa ctctcctccg aaacttattc ctctggaaat agtcgaaatc
     2941 cgaataggca gatgttgcaa cctcttagct atataaaaca tcgttgtctc accttcaata
     3001 ttagggctta tagccattac cacctcccta atgttcttat tgattattcg ctgttcaata
     3061 tcttcaatgt tcagttgatc aggaccaatt ccgtcaatcg gggaaatgag accaccaact
     3121 acgtgataaa atccctgaaa atgaccggtt tcttctattg ccataagatc gcggatggtc
     3181 tcaacaatgc acaggattcc attgtctctt ctcttgtcgg tacatatcga acacagaacc
     3241 tcatccccca gattgtggca ttcgtcgcac tccttcaaat catttcgaag ttttatgaaa
     3301 gattccgaaa aagcctgtac ccacatggca tccttgctta acagatggag agaaagacgt
     3361 agtgcagtct tcttaccgat accgggtaat gaactgaatg attccacagc tttccctatt
     3421 aattgagacg aatatttcat tggcaaaagg ttccttaact ctgtatatat atatatttgc
     3481 tacaaagata ttaaaatatt atatatattt aagttggata tggtttataa aaacgctcat
     3541 ttattaaagg ccataatgac tatattggat gggacattga attaaagtcg aaatttctac
     3601 cttggcactc cctaatatag agccaaccaa ggttacatta aaatgagata aaaccaaaca
     3661 gtgaggtgtg catggttaat tatttttaag ttaaggaaat taatttttat aattgggcga
     3721 tagttataat tttgtaggtg aaagtttttt cctttcattt tgcaaatgat agaaataatg
     3781 attgctaatt tatttacaaa ttcagttgta tgaccagata tgtacttaaa caaggattgg
     3841 ttttactgct ccttttctta ttcggtaacc tgcatggaca aataagcgga acagtacgtg
     3901 atgcttccgg ccagggcctg gttggtgtta ctattctagc aataggagag aataatggta
     3961 ccgtaaccga tattgatgga aattactctt tacgagtgaa tacagcaaat gctttggaat
     4021 tttcctatgt aggataccag acacaaaaga tcaatataga tggcagaaaa atgattgatg
     4081 tggtaatgca ggaagacgtg aaggccttga aggaagtggt tgtagtaggt tatggggttg
     4141 aacaaaagaa gactcttact tcatcagtag gtattgtcaa ttcagatgct atcaaagatc
     4201 ttcccgtacc taccattgac ggtgtgttgc aaggccaggc ctccggtgtt caggtagtac
     4261 agaattccgg cacacccggt ggaggcatgt ccgtaaggat aagaggtgta agttcgatca
     4321 gcggcagttc ccaacctttg tatgtcattg acggtatccc tgtcacaaca ggtgattttg
     4381 cgcaggtagg ctatgaaggc cagggagtaa accaactgtc agacctgaat cccggcgata
     4441 tcgaatcaat caccatcctc aaagatgcag cggctgcaac catctatggt gccagagcct
     4501 ccaacggagt tgttctgatc acaacaaaac gaggaagcag taagaagtct accattaatt
     4561 tatctgcctc ttatggttgg caacaaccct ggaggaaact ggaaatgctg aatgcccacg
     4621 attggaaact gtacaggaat gactatttcg gaacagaagt ttatacccct gagcaaatca
     4681 atgacaacac tatagataca aaatggcagg actacatttt ccgtaccgct ccaatctcca
     4741 attatgaatt atcaacatca ggcggtgatg aaaaaacacg attttacatc agtggaaact
     4801 tattcaatca aacaggtacc ctgataggaa gtgattacag gcgtatgaac ggaagggtca
     4861 atctggatca taaaatcaac aattctgtta ccattggtac aagtatcggc ttgtcctatg
     4921 ccaagactaa cagaatagaa ggagaccaat ccctgcatgg tgttttgccc aatggcattt
     4981 caacacccgc agtattcccc gtatataaca gcgatggttc ttataatcag gatggccctt
     5041 attctaatcc ggtttcgata gccaatgagg caaccaatga aaacttttct taccgtggat
     5101 tggggaatat atatctggac tggaaaatct ggaaagggct taccttttct actaaatggg
     5161 gtattgactt tatgaatttc agggagcatg cctttgaata taatactgtt cagggtagaa
     5221 aatacaacgg gctgggattc gaaacatata ctaatgttgg taatctggtt tccaataata
     5281 ctttgaaata cagttttgat ttacaggata acaggtttga agtactggca ggttattcat
     5341 tcgaaagaaa gaatgtagct agttcctata tcagaggtca ggattatgca gatgcaagtc
     5401 ttcagtacat caacagcgcc tctacaatta catctgcaag ctccggtgcg tctaatgaag
     5461 gtataagatc attgtttgga cgaatcaatt acaacctgaa ggataaatat cttgtatcac
     5521 tgacaggtcg tatggatgga tcttccagat tcagcgagaa caatcgtaat ggcttttttc
     5581 cggctgtttc agctgcctgg agacttggtg aggaagactt cttcaagtcc aatgtaattt
     5641 ctgagttgaa ggtccgtgga agcttcggac taacaggcaa tgatgatatc ccgcaatttc
     5701 tctatgccga attatatggc aacactactt atggaggcaa acctgctgtc tatcccagca
     5761 atattccaaa tcctgatctg aaatgggaaa caaccactca ggtgaatgca ggtttggaca
     5821 taggcttatt taaagacagg atttatctga atattgattt atatcgtaaa ttcaccaagg
     5881 atttgttgtt aagcagaccg ctgccattat cttccggttt ttctacaatc acagaaaata
     5941 tcggtgaggt ggagaacaag ggtattgaat tgggattaac taccgaaaac cttgcagatc
     6001 ggaaattaga atggaacacc agaatcaatt tctctttaaa caggaataaa ataaccaaac
     6061 tttacaacaa tcaacctata gacgatttgg gtcgtggaag taaccggatc gaagttggac
     6121 aacccatcgg tattttctat agctatgaat ggttgggagt agatccctca accggtgatg
     6181 tagtctttgc ggataagaat tttgatggta atattacttc cgatgatcga accattattg
     6241 gaaatcctca tcctgatttt atatatgcca tcaccaataa tttcaaatat ggcagatttg
     6301 atctttccat tttctttcaa ggttcccagg ggaatgacgt tatgaacggg tcgaggcttt
     6361 atctggaatc tttgcaaggt ggtgataatc aattggcagc aatcaacaga cgatggaaga
     6421 atgtaggtga cattaccgac attcccagag cgacaaacga ccccgttgca gctgccagca
     6481 acaaacgact ttcttccaga ttcctcgaag acggatccta cctcagattg aagaacctga
     6541 cattcggtta taattttaat acaaagaata tcatgaaagg caaaataagc tcattgagaa
     6601 tatacttcac gggtcaaaac ctgcttactt ttactaatta tacaggtctt gaccccgaag
     6661 tcaactatgt aggtgatgat aatgcacgaa tgggaacaga ttttttcact tatcctcagg
     6721 ccagaagttt ctcctttgga ttaaacgccg gtttttaata tgaaaaggat tttgatttta
     6781 ttagttctgg tatactcatt ctcatcttgc aatgtactcg atgtggaacc aaccaattct
     6841 atacctgcat ccgaagcatt taagactaaa gctgatttaa accgtggaat tttgggggct
     6901 tataattcat tacaaggtct aagttactac ggaagagcct atctgaccgt ttcagactta
     6961 tcggcagaca atctgcttta tcccccgagt gctaccactg ccgattacct cgatatcgac
     7021 aataaccata ttctgcctga aaatggagta attgaaggaa tttggggtgt agcatatgac
     7081 ggtttaaatg ttgctaacaa tgttattgct aaaattcctg agatgagttt tttgaatcag
     7141 gatgaaaaaa atgtagcact tgccgagtta tactttttgc gggctctgaa tcatttcaat
     7201 cttttgaatt atttcggggg aattcccatc aaaaccaagc ctaccatagg cctttccgaa
     7261 ataaatgcag cacgtaatag tcctgaagaa gtctatgcca ctatcatcca ggatctaact
     7321 tttgcttcta cttatctacc caaatcatcc gcatcaaaga tccgtgcttc acagggagca
     7381 gcaaatgcgt tgttggcaag agtgtacctg tataagaagg attatgccga tgccgtcatc
     7441 tatgccagta aggtaatcga tgacaataat tattccctga tgagtaatta ctcagatatc
     7501 tttgccgccg atgaatcaaa ggagtcaatt tttgagatcg attttacctc tcttgacaga
     7561 aacagaattg cagaatacaa ttttccgtta acccttaacg gaagaggcag tgtcggtccg
     7621 gaccccggaa ttgtcagtgc ctacgaagcc aatgacaaac gcaaggatgc ttccattgcc
     7681 tttgccggga ctatcccata tgcaatcaaa tatcatgacc ttgccacagg atcggacaat
     7741 gtaattgtct tgagattggc cgaaatgtat cttatacggg ctgaagccaa tgcacgttta
     7801 aatgggaata tcaatgatat tcggaaagat cttaatacca tacgaagtcg tgcagggtta
     7861 gaggaggccg ttgctaatag catagatgaa ctgcttcttg ccatcgaagc agaacgaaga
     7921 gtcgaactgg catttgaagg ccaccgttgg tttgatttgg tcaggacaga cagagcaatt
     7981 gatcttctgc ctaacgttac ccaaaagtac cagtatcttt ttccgattcc acaaagtgaa
     8041 cttttagcga ataccaatcc cggaatgaag caaaatccgg gttattaaat catatttaaa
     8101 attttaacac atgaaatcat taaaaaattt gttaccattg tttttacttg cagtacttgc
     8161 ggttaccgga tgtaaaaagg atgaccctgt caaaccaaaa ccggttgctt cttttacagc
     8221 cagtcaaact actgtatt
//
LOCUS       DAOQMC010000095         8220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3504_length_8220_cov_5.002817, whole genome shotgun sequence.
ACCESSION   DAOQMC010000095 DAOQMC010000000
VERSION     DAOQMC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3504_length_8220_cov_5.002817"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /gene="alaS"
                     /locus_tag="PKV31_05155"
     CDS             <1..1737
                     /gene="alaS"
                     /locus_tag="PKV31_05155"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNB92279.1"
                     /translation="ISFTIADGQLPSNAGAGYVIRRILRRAIRYYFSFLDCQEPLLAS
                     LVPVIAEQFKDVFPELHQQIDFVQRVIREEELSFLKTLDSGIRRFEQIVAESAGTLPG
                     EKVFELMDTFGFPVDLSVLMAQEKGRVIDMAGFEAALQLQKERSRASAQIENEDWVVV
                     NEVPEMSFVGYDNLNVETSIVKYRSFKAKDAIGHQVVLKETPFYAESGGQVGDTGFLM
                     FGHERVNVLNTKKENDLIIHTVDHLPQNAEQEVVAQVDEFRRNKITVNHSATHLMHAA
                     LRQILGPHVVQKGSLVNDVHLRFDFSHFAKVTDEEIAQIEDIVNEKIRENIPVIIKYM
                     DKDEALRTGAMALFGEKYGNIVRVVIIDPAYSIELCGGCHVGYTGEIGLFKITSESAV
                     AAGVRRVEAMTGQAALQYLNAYVEEVRQVRQLMGNPKEVITKLNDLIAENKSLRKEME
                     KMEADKALRLQHELSESVRRNSAGVNYIISRTELSDNKLLKNIGFNLARESGDALVAL
                     INCYDEKVTIQINISESLADKKIYNAGELVRQLAPLIGGGGGGQVGYATAGGNNVSGI
                     DSVLEQLTQLVE"
     gene            1953..2387
                     /locus_tag="PKV31_05160"
     CDS             1953..2387
                     /locus_tag="PKV31_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNB92280.1"
                     /translation="MNIFVAKLSGATTADGLKRLFEQFGEVSDVKIIIDRETNMSKGY
                     GFVEMPNVAEGHTAIAELNEAEVDGSIIVVKESQPRTGEKSGFSGGGNRGGNRSFGGG
                     NKFKKPGFGGGRSFDKKPGFGRSGDSDHRKGGYHSREDRNSY"
     gene            2675..4138
                     /locus_tag="PKV31_05165"
     CDS             2675..4138
                     /locus_tag="PKV31_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB92281.1"
                     /translation="MKIKTFIVLAIFCICTLPKHVFSINYTETKLERVAEDENEFDES
                     QIKIRLRKLNSSLDIRYTPEVKKFLQSYIKSYRSTSEQLLGRATMYFQYFDQALQKYN
                     LPSELKMISVLESSLNPNAISPAGAIGLWQFMPATAKDMGLDFNRVVDERRDPDKSAD
                     AAARYFLKLYSIFEDWNLTLAAYNCGPNRVQNAIDAAGGVKDFWAIRKYLPKETQNYI
                     PKFIAINYLFNYYNVHKLKPSFPELDLQITDLTSLYDKVDLKALSSKLGVSYDVMKVL
                     NPMYRQGYIPASNNPMNIILPMRYMNSFKSSIPRPDSKMLSVNESPLPSMSVGGNDYY
                     EMTYIAGPGENLERIAGRLGINSHLIRMWNGMTMNTIKEGQSLKLYILRNKQLDNSFA
                     KAELHPLTEQFVQVRTMMEDENFISLSHLDYTNDELVFTASSDQFVYHRLDERESIAD
                     LVSQYTGTTIQSILELNGFNRDNLPKPGDLIKVKKKQ"
     gene            complement(4234..4677)
                     /locus_tag="PKV31_05170"
     CDS             complement(4234..4677)
                     /locus_tag="PKV31_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB92282.1"
                     /translation="MRHISKKEMRPFNPYRPFEQFFNNELANFWSGDNFLESPAFNII
                     EENDKYVIELAAPGLEKEDFNIEIKDQILTIKVQKQESKDAETTDNNTKYIKREFSFQ
                     QFTRSFTLDNTIAQNEIEATYDKGILKVSLKKVSNPEIIKKIEIK"
     gene            complement(4710..5099)
                     /locus_tag="PKV31_05175"
     CDS             complement(4710..5099)
                     /locus_tag="PKV31_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB92283.1"
                     /translation="MTVIFKNVATPTMGQQTKASTRFIPLSNLINTGKGYILEMMVSG
                     FSKDDIEIDLKEDKLTIKGQMPKASADTIKFIRRGLKSGDFEATYTLSERIDKSAIDA
                     ECHNGLLRIVLPLKEEVIATPRKINIQ"
     gene            5484..6257
                     /locus_tag="PKV31_05180"
     CDS             5484..6257
                     /locus_tag="PKV31_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92284.1"
                     /translation="MNRFFVFLLSILFSFGAKAQVDSIHFQFQDTILKAGTTHTFLMT
                     VRENIQDALGFQFCLQFDPQNIGFKSIPKYYISGWTEDNMNIYPLGHPDYIACAVDVI
                     STPYTGKKLLEIQFDVFHDCRVKDVIRILSAYRFKEGDSYSKSEMFFENDTYPIATEY
                     LPFKLAGSKNIAPEKVESFPNPASNEVNMSFSSNISGKFPVTIYDNAGKSVGETIVEV
                     KSGDNKLTFNKALFGSAGSYIIQLNLGNRILTSRATFVN"
     gene            6966..>8220
                     /locus_tag="PKV31_05185"
     CDS             6966..>8220
                     /locus_tag="PKV31_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNB92285.1"
                     /translation="MRYLSALLFLVLFHFSGKGQCVFNLNDTIVADADFYIQGSVSGL
                     VNDDLSANQALCGVKLQFRHQFIGNLQIELVSPAGQKILLVGPPLAGNLTNGTLWDIT
                     FVRCIDIPSPDPAIPPQYDNGSPWAIGATYSGSYFPYAGCLEDFNVGSANGIWQLHII
                     NGSFYEGNLVSFSLIFCDPTGVDCNPCKANPGKFTLNERSFCQNDVNLLSFQPPIDYT
                     PDGQNTTLYNYEYVVTFNGIIQRIEYVNDWSVLSPGTYLIYNIAYLKEYESVVKSYIG
                     DPYSVLLNKINSTQICADVTGSAMQLNIFRVFDNPVRNILLCSGDSLVVSGKTYLADT
                     FIRDTLKSLYGCDSIINIDLRFTNIHINIEADTIDCINFLASIDTSGFRMDVPGGLVL
                     GYEWRDNGGNFISNAGGITVNTPGLY"
BASE COUNT         2422 a         1425 c         1730 g         2643 t
ORIGIN      
        1 atatcattta ccattgccga tggacagctt ccctccaatg ccggtgcagg ttatgttata
       61 agacgtattc tgcgtagagc tatcagatac tatttttcct ttcttgattg tcaggagcca
      121 ttgttggcaa gcctagttcc agtaattgcg gagcaattta aggatgtttt tccggagctt
      181 catcagcaaa ttgattttgt ccagcgggta attcgcgagg aagagctttc atttctgaaa
      241 accctcgaca gcggaattcg caggtttgag cagattgtag cagaatcagc aggtacattg
      301 cccggtgaga aagtatttga actaatggat acatttggtt ttccggtaga tctttcagtg
      361 ttgatggctc aggaaaaggg tagggtgatt gatatggcag gttttgaagc agctcttcag
      421 ttacagaagg aacgaagcag ggcttctgct cagattgaaa atgaggattg ggtagtggtc
      481 aatgaagtcc ctgaaatgag ttttgtaggg tatgacaatc tgaatgtcga gacatccatc
      541 gtaaagtata gaagttttaa ggcgaaagat gccattgggc atcaggtggt gttgaaagaa
      601 acaccattct atgctgaaag tggtggccag gtgggtgata cgggattttt aatgttcggc
      661 cacgaaaggg tcaatgtatt gaacactaaa aaagaaaatg acctgattat tcacacggtc
      721 gaccatcttc cgcaaaatgc tgagcaggag gttgtcgctc aggttgatga gttcaggcgg
      781 aataaaatta ccgtcaatca ctctgccacg catttgatgc atgctgcctt gaggcagatc
      841 ctgggcccac atgtagtcca aaaaggtagt ctcgtcaatg atgttcatct tcggtttgac
      901 ttttcccatt ttgccaaggt aacagatgaa gaaattgctc aaattgaaga cattgtcaat
      961 gagaagatac gggaaaatat tcctgtgatc atcaagtaca tggataagga tgaagcactc
     1021 cggacaggtg ctatggctct cttcggtgag aagtatggca acattgtccg agtagtgata
     1081 attgatcctg catacagcat tgaattgtgt ggcggttgtc atgtaggata tacaggagag
     1141 atcggcctgt tcaaaatcac ttccgaatcg gctgtcgctg ccggtgtgag aagagtagag
     1201 gcgatgaccg gccaagctgc attgcaatac ttgaacgctt atgtggaaga ggttaggcag
     1261 gtacgccaat tgatgggcaa tccaaaagaa gttatcacaa agcttaatga tttgattgca
     1321 gagaataagt cactcagaaa agaaatggag aaaatggagg cagacaaagc actccgatta
     1381 caacatgaat tatcggaatc agtgcgaagg aacagtgccg gagttaatta tataataagc
     1441 aggactgaat tgtcagacaa taagttattg aagaacatag gattcaattt ggctcgtgaa
     1501 tcgggcgatg ctttggttgc tttgataaat tgctatgatg agaaggtcac aatccagatc
     1561 aatatatccg aatcactggc agacaagaag atctataatg cgggcgagtt agtgaggcaa
     1621 ttggcgccat tgattggtgg aggcgggggt ggccaggtag gctatgctac cgccgggggg
     1681 aataatgtgt caggaatcga ttccgttttg gaacaactta ctcaactggt agaatgattg
     1741 aaaaaccgtt gggaagtatt gatatttaga atatttccaa agaagaaagt attttttaat
     1801 aaagcataat taaatgtttt ataatcaaat aaagcactta tctttgcatg gaattaatca
     1861 ccggcttgct attaaatcga cgttttcgtt tatttcaaaa tcattccggg tcgattttat
     1921 taatgtttta actaattttt taattttttc aaatgaacat ttttgttgct aaattaagcg
     1981 gtgctacaac tgctgatgga cttaagcgcc tttttgaaca atttggtgag gtcagtgatg
     2041 taaaaattat tattgatcgt gaaaccaata tgtccaaagg atatggcttt gtcgaaatgc
     2101 ctaatgttgc tgaaggtcac acggcaattg cagaacttaa cgaagctgag gtggacggca
     2161 gtattattgt ggtaaaggaa tcccaaccaa gaaccggtga aaaatcagga ttttccggag
     2221 gtggaaatcg tggtggaaac agaagtttcg gcggtgggaa taagtttaaa aaaccgggat
     2281 ttggtggtgg aagatccttc gacaagaaac ctggttttgg ccgtagtggt gatagcgatc
     2341 acagaaaagg cggatatcat agccgggaag acagaaactc ttattaatcc gcattaaaga
     2401 aagaaagaag cctgccataa tttatggcgg gcttttcttt tttaacatag aatagtagat
     2461 atattgatct ttaaatgtaa atcctgtaaa tgcctggaaa gcaggcaggc gtttctgcga
     2521 ctgtaggtca atgtgtttca tattctaaaa atataatata tgttaatatt ttttcttttt
     2581 ctgctttaaa ctcgtacgga gactgaattt gtcctacttt tgcctttgca atacgttgca
     2641 ctaggaattt attttgttaa ctaaattact gacaatgaaa ataaaaactt tcattgtact
     2701 agcaattttc tgcatttgta cattaccaaa gcatgtattc tcgataaatt atactgaaac
     2761 aaaactcgaa agagttgcgg aagatgaaaa tgaatttgat gaaagtcaaa tcaaaatacg
     2821 gttaagaaag cttaattcct cccttgatat cagatatacc ccggaagtta agaaattcct
     2881 tcagtcctat ataaaatctt atcgaagcac gtccgagcaa ttgctgggta gagcgacgat
     2941 gtattttcag tattttgatc aggctctcca gaagtataat ttgccctctg agttaaagat
     3001 gatttctgtt ctggaatcct cgcttaatcc taacgcaatc agtcccgctg gtgcaattgg
     3061 actatggcag tttatgcccg caaccgcaaa agatatgggt ctggatttca atcgggttgt
     3121 tgatgaaagg agagatcctg ataaatccgc cgatgctgca gcccgttatt ttttaaagct
     3181 gtactctata tttgaagatt ggaatcttac ccttgcagct tataattgcg gacctaacag
     3241 ggtacaaaac gctatcgatg ctgcaggagg tgtcaaggat ttttgggcaa tccgaaagta
     3301 tcttcctaaa gagactcaga attatattcc aaagtttatt gcaatcaatt acttatttaa
     3361 ctattacaat gtccataaat taaaaccttc cttcccggag cttgatctgc agattacaga
     3421 tctgactagt ttgtatgaca aagttgacct gaaggctctt tccagtaagc tgggtgtatc
     3481 ctatgacgtt atgaaggtac tgaatcctat gtatcgacag ggttacatac ctgcttccaa
     3541 caatcctatg aatatcattt tacctatgcg gtatatgaat tcttttaaaa gttcaattcc
     3601 acgtccggat agcaaaatgt tatcagtaaa tgaatccccc ttgccctcta tgtccgtggg
     3661 tggaaatgat tattacgaaa tgacctacat agccggtcct ggtgagaatc tggaaagaat
     3721 tgccggtcga ctcggcatca atagtcatct gatcagaatg tggaacggta tgactatgaa
     3781 taccatcaag gaaggtcagt cgttgaagtt atatatactt agaaacaagc aattggacaa
     3841 ttcgtttgct aaagctgaat tacatccatt gactgagcaa tttgtccagg taaggactat
     3901 gatggaggac gagaatttca tttcgttatc gcacttagat tacacaaatg acgaattggt
     3961 ctttactgct tcatctgatc agtttgtata tcatcgactt gatgagcgag agtcaatagc
     4021 tgatttggta agtcaatata ccggaactac catccagagt atccttgagt tgaatggttt
     4081 taacagagat aatctgccaa aacccggaga tttaattaag gtgaaaaaga aacaataaaa
     4141 aaagcccgga agtccgggct ttttcattat tacttcaagg tttgttgcta catccaattt
     4201 tatagattga tagcatatcc gggatatcaa tgcttatttg atctcaattt tcttgatgat
     4261 ttccggattg gaaacctttt tcaacgaaac ctttaatatt cctttgtcgt aggttgcctc
     4321 tatctcattt tgagctattg tattatcgag ggtaaagctg cgtgtaaatt gctggaaact
     4381 gaattccctc ttgatatact tcgtattatt gtcagtggtc tcagcatctt tcgactcttg
     4441 tttttggacc ttgatggtca gaatttggtc tttgatttca atattgaaat cctctttttc
     4501 aagtcccggt gccgccaatt caattacata cttgtcattc tcctcaataa tgttgaaagc
     4561 gggagactca aggaaattgt cgcctgacca gaaattggcc agttcgttgt tgaaaaattg
     4621 ttcgaaaggc ctgtaaggat tgaaaggcct catctctttt ttgcttatgt gtctcatgat
     4681 tattaatgat ttaagaaatg aatgaattat tattgaatgt taatttttct gggagttgcg
     4741 atgacttcct ccttcagggg gagaacgatt ctaagcaatc cattatggca ttctgcgtca
     4801 atggcgctct tgtcaatcct ttctgaaaga gtataagttg cttcaaaatc tcctgatttg
     4861 agtcctcgtc taataaattt gatggtgtca gctgatgctt taggcatctg gccctttatg
     4921 gtaagtttgt cttcttttaa gtcaatctct atatcatcct tagaaaatcc gctgaccatc
     4981 atttccagga tatatccctt cccggtgttt ataagattgc ttaatggaat aaaccgagta
     5041 gatgccttag tttgttggcc cattgtcgga gttgcaacat ttttgaatat tactgtcatt
     5101 tttttattat tttgttacac tcacaatatt gcaatatttg ttcctttgat ttaaaagtgc
     5161 ctaaaagtca gtttgttata ccaaaacagt gacataatga cacttaataa gaaaacagtg
     5221 cgccaaaatg acgtattttt tttagttgtt taatatttaa atgaaattta cccaatatta
     5281 cgaccaggag gctctaagct cattcgtatt atcattaaaa ttaatgtgta tttatataat
     5341 taacttatat ttgcaagttg gattatcgaa agatttttat atttttgcat ttattttcgc
     5401 atcttgaggc agcacattta taaaaatcat agtatatcta agcgccctta gtattgaatt
     5461 aatttatacg caaaaattta gttatgaaca gattttttgt ttttttactt agtatcttgt
     5521 tttctttcgg agctaaagcc caggtagatt ccattcattt tcagtttcag gatactatat
     5581 tgaaagccgg taccacccat acttttttga tgactgttcg ggagaatatt caggatgcat
     5641 tgggatttca attttgtctt cagttcgatc cgcagaacat cgggtttaag agtatcccga
     5701 aatattatat ttcaggatgg acagaagata atatgaacat ttatcccctg ggtcatccgg
     5761 attatatagc ttgtgccgtg gatgtgattt ctactcctta cacgggtaaa aaactacttg
     5821 agatacaatt tgacgttttt catgattgtc gcgttaagga tgtaatcagg attctttctg
     5881 cctatcgttt taaggaaggg gattcctata gtaaaagtga aatgttcttt gaaaatgata
     5941 catatccgat tgcaacagaa taccttccgt ttaagttagc cggaagcaag aatatcgctc
     6001 cggaaaaagt ggaatcgttt ccaaatcctg catccaatga agttaatatg agtttttctt
     6061 ctaatatatc aggaaagttt ccggtgacta tttatgataa tgccggcaaa tcggtaggag
     6121 agacgatagt tgaagttaaa tcaggggata ataagctgac attcaacaaa gcactgtttg
     6181 gttctgcggg gagttatatt attcagttga atcttggtaa tcgcatcctt acctccaggg
     6241 cgacctttgt gaattaatca gatcaacttg taggaataat ctgaattttt aatcaaatct
     6301 gttagaaaag gaactgccat ttggaagttc cttttttata tttctcctgt tttacacttt
     6361 accaattaca ttttactaat tcaagatctt taaggttgac ggggcaaaag tgttttatcc
     6421 gacaacaatt taaaccggtc aactaatgta ccctgtcttt ttatctgcta atggaaaatt
     6481 cttgcaggtg ccagttttta ttgtaggaga aattctctta ggcccgcaaa cgacacccat
     6541 atctctcaac cgtataagga attcaatgac atgacaaggc cttgttttgt tattcctttt
     6601 tctgaaatta agtaaatagt gagtattcaa aaaaatacat tggttgtcat gggatcatga
     6661 tcccttgaag ttggaaccat tcctttgaca ttatctcctt gctataggaa caatttatca
     6721 ggtgattcag gtctcatgaa aggaacataa ttgatcagta acatagtcag agtaatgtga
     6781 aaacttaagt tattttttat aaggaaaagc agcctaatct atctttggga tatacattat
     6841 tatattgatg cttttgaaaa atgataattt aaaggctgtg gataaaagtc attaacaggg
     6901 ttgctttacc caaatcgttg aaattagatg tatatttgct tattaatccc ataatcatac
     6961 aaaagttgag atacctttct gcacttttgt ttttggttct ttttcatttt tccggaaaag
     7021 gccaatgcgt attcaacctc aatgacacta ttgtggcgga tgcggatttt tatatccagg
     7081 gaagtgtcag cggactggta aatgatgatc tttctgccaa tcaggcgctt tgtggggtca
     7141 agcttcaatt cagacaccaa ttcatcggga accttcaaat tgaattagtt tctcctgccg
     7201 gacaaaagat actactggta gggccacctt tggcgggcaa tttgaccaat ggtacattat
     7261 gggacattac ctttgtcagg tgtattgata tcccatcacc ggatccagca attccaccgc
     7321 aatatgacaa tggttcaccc tgggcaattg gggcaactta ttccggttca tattttccat
     7381 atgccggatg tcttgaggat ttcaatgtgg ggtctgcaaa tggtatttgg cagttgcata
     7441 ttattaatgg gtctttttac gaaggtaacc ttgtcagttt tagtcttatt ttttgtgacc
     7501 ctaccggcgt tgattgtaat ccatgcaaag ccaatcctgg aaaatttacc ttgaatgaaa
     7561 ggtctttctg tcaaaatgat gttaatttac tttcatttca gccgcctatc gattatacgc
     7621 ctgatggcca gaatacgaca ttatacaact atgaatatgt tgttaccttc aatgggatta
     7681 ttcaaaggat agaatatgtg aatgactggt ctgtcctaag tcccggtacc tacctcattt
     7741 ataatattgc ttatctgaaa gaatatgagt cagtagtaaa atcctatata ggagatcctt
     7801 acagtgtttt attgaataaa atcaattcta cccaaatttg tgctgatgtt accggcagtg
     7861 caatgcagtt gaatatattc agggtctttg ataaccctgt aaggaacatc ctgctttgta
     7921 gtggggatag tctggtggta tcgggtaaga cttatttggc tgataccttc ataagagata
     7981 cgcttaagtc attatatggt tgcgacagta tcattaatat tgatttgagg tttaccaata
     8041 tacatattaa tattgaagca gacactatcg attgcatcaa cttcctggct tcgattgata
     8101 cttccggatt tcgaatggat gtgcctggag gcttagttct cggatatgaa tggagggata
     8161 atggcggcaa cttcatctcc aatgccggtg gcatcacggt caatacaccg ggattatatt
//
LOCUS       DAOQMC010000096         8179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3525_length_8179_cov_4.995938, whole genome shotgun sequence.
ACCESSION   DAOQMC010000096 DAOQMC010000000
VERSION     DAOQMC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_8179_cov_4.995938"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PKV31_05190"
     CDS             <1..894
                     /locus_tag="PKV31_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92286.1"
                     /translation="KKGDIREVNPGIWFQQEIRLDNGLEVDLGARLDYFRNEYIDHLE
                     NNRKLTASSMIFSPKVRLNYSVNSHWKIYWYGGKGFHSNDTRVAVEKEGKEVVTPAWG
                     SDLGTIVKLGEKALLQSALWYLYMQQEFIYVGDEGIVEPSGRTQRLGLALSLRYQPTQ
                     RLYFDIDLNLSRPKAIDEAQGEDFLPLAPIFTSTGGVSYKTPNGWNGSLRYRWMGNRP
                     ANEDYSTVARGYFITDLSVNYSKPRWEVGLVVQNAFNRRWKETQFDTESRLKGEIEPV
                     TEIHFTPGSPFMARLNFSCFF"
     gene            complement(943..1677)
                     /locus_tag="PKV31_05195"
     CDS             complement(943..1677)
                     /locus_tag="PKV31_05195"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HNB92287.1"
                     /translation="MKHFIPMIALMAIFAIGCKTANKVAEPTVTTGEKPKVHKYFSVD
                     QGPCFGRCAVYDIVLLSDSTLRLNGKNYTNYIGHYSKKLSGREFDSLMDLYKAVKLDT
                     FRSEYNNGIVDLSSVTYYFYDDYNKVVKKIFTQGLYPDPLNSLSSAMSMHISKLGWER
                     DNNADTVNPDELIVQVNEGVNIQDIVDENWRYRLFIKEELSRGKIYLLGFDKSTIEQD
                     RLINVLKTNKGVMFVEKNNRVELRNR"
     gene            complement(1687..2949)
                     /locus_tag="PKV31_05200"
     CDS             complement(1687..2949)
                     /locus_tag="PKV31_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNB92288.1"
                     /translation="MSVYYKLCSIVILLIIGQVGVIGQERCGEDWISMQAASMVPDYE
                     QRVTNNTKRLREAIRSEGLTIREPMVIPIVVHVVYNNDNEKISESQVLSQIEQLNADY
                     EHLIIPSTIPAEFAHTWQDAGIRFCLAARDPEGNPTNGIVYRQTSVKNIGIKANGQGR
                     KFIHHNYLGGSDAWPTSRYLNIWVCRLDEYLGYATGPDNIAFSDEDGVVINYSNFGNI
                     GTVDPKSRYNMGRTATHEVGHYLGLLHLWGKNASGCGDDLVEDTPQQGTPHYECPDYP
                     QLSCFTSNMFMNFMDYVNDPCMYFFSKGQGERMRATVSTFRPGLLSGGLTACDTSGIA
                     TTPLKDRIRLKSTLVDGFITLLQTRPGNEVLNIELFDESGRLIKKYTKIPQSEFNLYY
                     DRMLSPGMYFLRISTKDEHTTLKVFIKG"
     gene            complement(3041..4285)
                     /gene="hutI"
                     /locus_tag="PKV31_05205"
     CDS             complement(3041..4285)
                     /gene="hutI"
                     /locus_tag="PKV31_05205"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNB92289.1"
                     /translation="MANTLDIIRASFIWGTYITTPGKPVSGHSLNELPYLENGFISIV
                     DGRIRRIGLMSEYSPVAGRTVMDASGAHILPAWCDSHTHLVHAASREKEFEMRLRGKS
                     YEEIAAAGGGILNSADVLRAMEEEELFDLSYRRFQAVQKLGTGAIEIKSGYGLDFDSE
                     VKMLRVIRRLKEKNEIPVKSTFLGAHAFPREFKDNHPGYISLIIDRMLPYIADHGLAD
                     YIDVFCDRGFFTPEQTARIMDAGAKYGLKPKIHANELAVSGGVEIGVAHNAVSVDHLE
                     EMDEHSIEALSGSDTIGTMLPGCAFFLGIPFPKARTMIEADTKLALATDYNPGTAPSG
                     NMNMVVSLACIRMKMTPQEAINAATINGAFAMESQQMVGSITPGKLANLIITGPMPSL
                     AYFPYSFGDHPMDKVLINGRVI"
     gene            complement(4285..5292)
                     /locus_tag="PKV31_05210"
     CDS             complement(4285..5292)
                     /locus_tag="PKV31_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB92290.1"
                     /translation="MRLSIILFLFLTGAGISSAQSPSYFEMSYLEAKQKASKSNKLCA
                     VVFTRDLSKSSAYFTEVAFEDPEVKKAVTDSYLIAASNVRDFDSKSLLKKWGLDQAPS
                     IAIVNAKGDVVGTANYGMSKSELARFLQYYSIPANAGKKSSMDDGGFEGEVAAWEGFK
                     GKDMDGKMAVNNPVTAASEAKMGIVPAATQEKPVENVQPPKDVEKEAIAVRGEVPVVA
                     ENNQAVAEKPKQEPKAVAPQTHTQPATQAKNPEPTHHDAGEYKFLVQAGIFSVETSAR
                     TLVSKIEAQGGNAYIEPVVQPDKTVYKVIAGKYYTEKEARDFIAKLKDAGIASFIKPV
                     N"
     gene            complement(5435..6070)
                     /locus_tag="PKV31_05215"
     CDS             complement(5435..6070)
                     /locus_tag="PKV31_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB92291.1"
                     /translation="MFSGKKIFILAPHTDDGELGCGGTIARAIEEGAEVYYIAFSTAE
                     QSVPKEFPSNQLEVEVREAVTLLGIRPEHLFVYKYEVRKLNYVRQEILELLMELKKRI
                     NPDIIFVPSMRDIHQDHSTVAQEAIRAFKTKTIYGYELIWNNIDFRTNAFIVLEQRHI
                     DQKINALSAYHTQAGKDYMDPDFIRSLARVRGTQIGEVYAEAFELVRTVIS"
     gene            complement(6251..7345)
                     /gene="wecB"
                     /locus_tag="PKV31_05220"
     CDS             complement(6251..7345)
                     /gene="wecB"
                     /locus_tag="PKV31_05220"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB92292.1"
                     /translation="MKKVYFVVGARPQFIKAAPILVAMRQYPVAMKLIHTGQHYDYNL
                     SDIFFDELEMPKPDYNLDCGSGTSTEQYLSVLSRLTQVLMHDRPDLIVVFGDTNSTGA
                     AALSARFNGIAVAHVEAGLREFDKNIPEEINKLVADALADLYFCPSTTAVEQLEGCKA
                     DGRIIHSGDPVIDLLVTGSKYTDRPELPAEYGLTPGKYIFATCHRQSNTNHQSALENV
                     LRIFANAHLPVLFPVHPRTMKAIHRFGLEHYLRDKNIKVIEPIGFWVTQALISQCERV
                     LTDSGGIIKEAYFHGKYVLVLDNQTEWVEVIREGKGIVCGTDYKMAIDNLKNKPDTSS
                     PNTALGDGTASIKIAKGIMEFLEGIHSPSV"
     gene            complement(7342..>8179)
                     /locus_tag="PKV31_05225"
     CDS             complement(7342..>8179)
                     /locus_tag="PKV31_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92293.1"
                     /translation="GNRLKKKFPHLVWIADFRDLPVEPIYKLQFFPHFHHRLYHRLFC
                     RVDVMTTISQGLADQLKRYDRPVFSVMNGVDVDFRFPQPNTTNYFTLVYTGSMYSDER
                     NPWPVFAVLNDLIREGKVDRDKIRIEYAGKDSAFWREMAQKAQLGDILEDYGLVGGDQ
                     AGRLQQNACINLLLTMASDQLSGILTGKYIEYLQAGSPILCIVKNQHDDFLTQELSRL
                     NAGLSVSDNEEEYPVIGQFILEKYLSWQVTGMNEKSSNQSAILQHYDSDRLISAIEPY
                     LK"
BASE COUNT         2216 a         1943 c         1719 g         2301 t
ORIGIN      
        1 aaaaaaggcg atatcaggga ggtcaatccg ggaatctggt ttcaacaaga aatcagattg
       61 gacaatggtc tggaagtaga tctcggagcc agactggatt atttccgcaa tgaatacatc
      121 gatcatcttg agaacaaccg aaagcttaca gcctcatcca tgatcttcag cccgaaggtt
      181 agattgaatt attcggtcaa tagtcactgg aaaatatact ggtacggagg gaagggattt
      241 cacagcaacg acacgagggt agctgtggaa aaggaaggta aggaagtcgt cacgccggct
      301 tggggtagtg acctgggtac catcgtgaag ttgggtgaaa aggctctatt acaatctgca
      361 ttatggtacc tgtatatgca acaagaattt atctacgttg gagatgaagg tattgtggag
      421 cccagtggca ggacccaacg attaggcctg gcgctttcac tgagatatca accaacgcaa
      481 cgactctatt ttgatatcga tctcaacctg agtaggccaa aagcgatcga cgaagcccaa
      541 ggtgaagact ttctccctct ggctccaatt tttacaagta ccggtggggt ctcctataaa
      601 accccaaatg gctggaatgg gagtcttcgg taccgatgga tgggcaaccg tcctgccaat
      661 gaagattatt caacggtagc caggggttac ttcattaccg atctttcggt caattattca
      721 aagccacgat gggaagtggg tttggtcgtt caaaatgctt tcaacagacg gtggaaggaa
      781 acccagtttg acacagaaag tcgcctaaag ggcgagattg agcctgtaac tgaaattcat
      841 ttcacgccgg gcagtccatt catggcccgg ttgaatttct cctgtttctt ttgaaatttt
      901 tacctcaaaa gacattagtt acaacgaggc tgcagccctg aatcaacgat tccgcaactc
      961 cacccgattg tttttctcaa caaacatcac tcctttgttt gtcttgagca cattgatcag
     1021 ccgatcctgt tcgatggttg acttgtcgaa tccaaggaga tatatctttc ccctggacaa
     1081 ctcttctttg atgaaaagcc tgtaacgcca gttttcatca acaatatcct gtatattcac
     1141 accttcattg acctgaacga taagttcatc cggattgact gtatctgcat tgttatctct
     1201 ctcccaccct aatttactga tgtgcatact catggcagat gaaagactat tgagcggatc
     1261 aggatacaat ccctgtgtaa agatcttctt caccaccttg ttataatcat cataaaaata
     1321 ataagtcacg gatgacaaat caactatacc gttgttatat tcgctgcgga aagtatccaa
     1381 tttgactgct ttgtaaaggt ccatcaacga gtcgaattct ctaccggaca attttttact
     1441 gtaatgaccg atatagttgg tataattttt accattgagc ctaagtgtgg agtcactcaa
     1501 cagaacaata tcgtacactg cacatcgccc gaagcagggt ccctgatcaa cagaaaaata
     1561 tttatgtacc tttggcttct cgccagttgt aactgtcggt tctgccacct tattagcggt
     1621 tttgcaccca attgcgaaaa ttgccatcaa agctatcatc ggaataaaat gtttcatctt
     1681 catgatttat ccttttataa atacttttaa cgtcgtgtgt tcatcttttg tgcttatcct
     1741 taggaaatac atacccggag aaagcattct gtcgtaatac aaattaaact cactttgggg
     1801 aattttagta tattttttaa tcaatcttcc cgattcatca aacaattcga tgttcagaac
     1861 ctcattgccc ggccgggttt gcaaaagagt aatgaatcca tccacaaggg tacttttcaa
     1921 cctgatcctg tctttcaacg gtgttgttgc tattccggac gtatcgcagg ctgtcaaccc
     1981 gccggataaa agcccgggcc tgaaagtact gaccgtagca cgcatccgtt cgccctggcc
     2041 ttttgagaag aagtacatac aaggatcatt gacgtagtcc atgaaattca tgaacatatt
     2101 ggaggtaaaa catgacaatt gtggatagtc aggacattca taatgcggag tcccttgttg
     2161 tggtgtatct tccaccaaat cgtccccgca accagaggca tttttgcccc aaagatgcag
     2221 cagccccaga taatgaccca cttcatgggt tgctgttcta cccatattat acctgctttt
     2281 cggatcgaca gtaccgatgt ttccaaaatt ggaataattg atcacgacac catcctcatc
     2341 actaaatgct atattatcgg gtccggtggc atagccgaga tattcatcca atcggcatac
     2401 ccagatatta agatatctgg acgtcggcca tgcatcactg ccccccagat agttgtgatg
     2461 aatgaattta cgcccttgcc cattcgcctt aattccgata tttttgacgg aagtttgccg
     2521 gtagacaata ccgttggttg gattgccttc cggatccctg gcagcaaggc aaaaacggat
     2581 gcctgcatcc tgccacgtgt gtgcaaattc tgcaggtatg gtggatggta tgataagatg
     2641 ctcataatcg gcattgagtt gttcgatctg cgataaaact tgtgattccg atatcttctc
     2701 attgtcatta ttgtatacca catggacgac aatcggaatg accatcggtt ctctgattgt
     2761 caggccttcg gagcgaatgg cttcccggag acgttttgta ttattcgtca ctctttgttc
     2821 gtaatccgga accattgatg ctgcctgcat ggagatccaa tcttcaccac atctctcctg
     2881 cccaataacc ccgacttgcc cgataataag caggattaca atggaacaca atttataata
     2941 tactgacatc agaacacgca atattgaata aagaaacgaa aagaagtgat aaatggttgc
     3001 atcgcattga gcagttggac ttcttttccg actatgaaac ttaaatcacc cggccattga
     3061 tgagcacctt gtccatagga tgatcgccaa aactatacgg gaaataagcc agggagggca
     3121 tgggcccggt aatgatgagg ttggcaagct tgccaggcgt tatgctacca accatctgtt
     3181 gcgactccat agcgaatgct ccattgatgg ttgcggcatt gatggcttcc tgaggagtca
     3241 tcttcatccg gatgcatgcc agtgacacca ccatgttcat attgccggat ggtgcagtac
     3301 cgggattgta gtccgtggca agggccagtt tggtatctgc ttctatcatg gtacgtgcct
     3361 tagggaaagg tatgcctaaa aagaaagcgc aaccgggcag catggtgccg atggtatccg
     3421 agccggacaa tgcttctata gaatgctcat ccatctcctc caggtgatcg acgctgacgg
     3481 cattgtgcgc cacacctatt tcaacgccac cggaaacagc gagttcattg gcatggattt
     3541 tcggcttcag accgtacttt gcaccggcat ccatgatcct tgctgtctgc tctggtgtaa
     3601 agaacccacg atcacagaag acatcaatat aatcagccag accatgatca gcgatgtatg
     3661 gcaacatcct gtctataatg agtgaaatat atcccgggtg gttgtccttg aattcacgag
     3721 gaaaggcatg cgcaccaagg aaagttgact tgacaggaat ctcgtttttc tccttcaggc
     3781 gacgaatgac acgtaacatc tttacctcgc tgtcaaaatc aagaccatac ccactcttga
     3841 tctcaatcgc tccggtaccc agtttttgca ctgcctgaaa gcgccggtaa ctaaggtcga
     3901 aaagttcttc ttcttccatt gctctgagca cgtcagccga attgaggata cccccgcctg
     3961 cagcggctat ttcttcataa gattttccac gcaggcgcat ctcgaattcc ttttcccggc
     4021 tggcagcatg caccaggtga gtatgactgt cacaccatgc cggcagaata tgggcacccg
     4081 atgcatccat aaccgtacgt cctgcaacag gtgaatactc ggacatcaga cctattctcc
     4141 ggatcctgcc atccacgatg cttataaagc cgttctccaa ataaggaagc tcattcaggc
     4201 tgtgtccgga aaccggcttc ccgggtgttg ttatgtacgt cccccatatg aagcttgccc
     4261 gaatgatatc gagagtattt gccatcaatt gacaggtttg ataaaggagg cgataccggc
     4321 atctttcagt ttagcgatga aatcacgtgc ctccttctcg gtataatatt taccggcaat
     4381 taccttatag acagtcttat caggctgaac gaccggttcg atatatgcat tacccccctg
     4441 agcttcaatc ttggacacca atgtccttgc actggtctcc acactgaaaa ttcccgcctg
     4501 aaccagaaac ttatattctc cggcatcatg atgtgtcggt tcgggatttt tcgcctgtgt
     4561 tgcaggttgc gtatgtgtct gcggggcaac tgcctttggt tcctgctttg gcttttctgc
     4621 tactgcctga ttattttcag caacaacggg tacctctcct cttacagcga tggcttcctt
     4681 ttcaacatcc ttcgggggtt gaacattctc aaccggcttt tcctgagttg cggcaggcac
     4741 tatgcccatc ttcgcctcag aagcagcggt aacagggttg ttgaccgcca tcttgccatc
     4801 catgtctttg cctttgaatc cttcccaggc agcaacctct ccttcaaatc caccatcatc
     4861 cattgaggat tttttaccgg cgttggcagg gatgctataa tactgcaaga acctagccaa
     4921 ttcggatttt gacatgccat aattggcagt tccgacaaca tcacctttgg cattgacaat
     4981 ggcgatagaa ggcgcctgat ccagacccca tttcttgagc agagatttgc tatcaaagtc
     5041 acgtacattg gaggcagcaa tgagataact atcagttacc gctttcttga cttcgggatc
     5101 ttcaaaagct acttcagtaa aataggccga acttttgctc agatctctcg taaaaacaac
     5161 ggcacataac ttattactct tagatgcttt ttgcttagct tccaggtaac tcatttcaaa
     5221 ataagaagga gactgggctg acgaaatgcc tgcacctgta aggaaaagaa acaaaataat
     5281 ggagagacgc ataatgtggt tttataaaaa atggtcaaat tccgttccga aaacaattcg
     5341 gtatcaagtg caaatataaa ttaccggatt gatttaaaga aaattttaac aaatatatta
     5401 catatatatt aaatatgtaa ttttcaaata atctttatga tataacagtt ctgaccaatt
     5461 caaatgcttc ggcatagacc tctccgatct gtgtgccgcg tacccttgcc agtgaccgta
     5521 tgaaatcggg gtccatataa tctttacctg cttgcgtatg ataggcactt aatgcgttga
     5581 ttttctgatc aatatgtctt tgttccagga caataaaggc attggtccga aaatcaatat
     5641 tattccaaat caactcataa ccgtatatgg ttttggtctt gaatgcgcgt attgcttcct
     5701 gcgccacggt actgtgatcc tgatggatat cgcgcatact cggtacaaag atgatatccg
     5761 gattgatcct ttttttcaac tccatcagca attcaaggat ttcctgacga acataattca
     5821 gtttgcggac ttcatattta tatacaaata aatgctccgg cctgatgcct agcaaagtaa
     5881 ctgcttctcg gacttctacc tccagttgat tggaaggaaa ctcctttgga actgactgct
     5941 cagccgttga aaatgcaata taatatactt cggctccctc ttcaatggcc cgtgcgatgg
     6001 tgccaccaca gcccaactca ccgtcatcgg tgtggggagc aaggatgaat attttctttc
     6061 cggaaaacat ggtattaaca aagataatta aaataacaga aactgaaata gcgtgaattt
     6121 gttttgaagc atcatgattt gtacttatca ggccacttca ggtataatgg aaagtttgaa
     6181 aaattgagga tcaaatgtct gacaataatg acctgtcctt tggcatagtc actttggtct
     6241 tcagtaaata ttacactgaa ggactgtgta tcccctccag aaattccatt attccttttg
     6301 cgattttgat cgaggcagtt ccgtcaccca aagcagtatt gggagaggat gtatccggct
     6361 tgttttttag attatctatg gccattttat aatcagtgcc acatacaata cctttacctt
     6421 ccctgattac ttccacccat tccgtctgat tgtccagaac aaggacatat tttccatgga
     6481 agtaagcctc cttgatgata ccgcccgaat ccgtcaatac tctctcacat tgactgatga
     6541 gggcctgtgt aacccaaaag cctattggtt cgatcacctt gatattcttg tcccggagat
     6601 aatgttccag cccaaatcta tggatagctt tcattgtcct gggatgaacc ggaaagagaa
     6661 ccggcaggtg ggcattggca aagattcgca atacattttc caaagccgat tgatgattgg
     6721 tattactttg cctgtggcag gttgcaaaaa tatatttgcc cggtgtcaga ccatattcag
     6781 ccggcaattc aggcctgtca gtgtatttgc tgccggtaac aagcaggtcg atcaccggat
     6841 ctccgctatg gatgatcctt ccatccgcct tacatccttc caactgctct actgccgttg
     6901 tgctcgggca gaaatacaga tcagccaagg catctgccac caatttgttt atttcttccg
     6961 gaatgttctt gtcaaattcc ctcaatcctg cctctacatg ggccacagca atcccattaa
     7021 atctggcgct taaggctgct gcaccggtcg aattggtatc cccgaataca acaatcagat
     7081 caggcctgtc atgcatcaac acctgtgtca accgcgacag gacactcagg tattgctctg
     7141 tggaagttcc ggagccacaa tccaggttat aatccggctt aggcatctcc aattcatcaa
     7201 aaaatatatc ggacagatta taatcataat gctgaccggt atggatcagt ttcatcgcca
     7261 ccggatattg tcgcattgcc accaggatag gtgcagcctt gataaactgt ggcctggcac
     7321 ctacgacaaa gtaaactttt ttcatttcaa ataaggttca atcgctgaga tcagccggtc
     7381 cgaatcataa tgttgcagga tggccgattg gttggaagat ttttcattca ttcctgtcac
     7441 ctgccaggac agatatttct ctaagataaa ttgcccgatg acaggatact cctcttcatt
     7501 gtccgaaaca cttaatcctg catttaatct tgataattcc tgtgtcagga aatcatcatg
     7561 ctgattttta actatacaca atattggact gccagcctgc aggtattcga tgtattttcc
     7621 cgtaaggatg ccactcaact gatcagacgc catggtgagc agcaggttaa tacaggcatt
     7681 ttgctggagc cggcctgctt gatctcctcc gaccaggcca taatcctcca ggatatcacc
     7741 caattgagcc ttttgcgcca tttcacgcca gaatgcactg tctttaccgg cgtattcaat
     7801 ccgaatctta tcccgatcaa cctttccctc cctgattaga tcgttcaaca ctgcgaaaac
     7861 cggccatgga ttgcgctcat cagaatacat cgaaccggta tagaccaagg tgaaataatt
     7921 tgtcgtattg ggttgaggaa atctaaagtc gacatccaca ccattcatta ctgaaaaaac
     7981 aggcctgtca tagcgtttca gctgatcagc cagaccttgg gagatagttg tcattacatc
     8041 taccctgcag aatagtctat gatacaggcg atgatgaaaa tgaggaaaaa attgaagttt
     8101 ataaatgggt tctaccggta aatcccgaaa gtcggcgatc caaaccaaat gaggaaattt
     8161 ctttttgagc cgattgccg
//
LOCUS       DAOQMC010000097         8127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3552_length_8127_cov_5.364470, whole genome shotgun sequence.
ACCESSION   DAOQMC010000097 DAOQMC010000000
VERSION     DAOQMC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3552_length_8127_cov_5.364470"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /gene="infC"
                     /locus_tag="PKV31_05230"
     CDS             complement(<1..352)
                     /gene="infC"
                     /locus_tag="PKV31_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNB92294.1"
                     /translation="MAKKERQDKGAEKAQFRINEQIRIPEVRLIGEDFEVISDVAGHN
                     VEAGIYSTPLLLDWARQAGLDLVEISPNATPPVVKIIDFQKFLYNKKKKERELKAKTV
                     KTVIKEIRFGPNTDD"
     gene            775..1386
                     /locus_tag="PKV31_05235"
     CDS             775..1386
                     /locus_tag="PKV31_05235"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNB92295.1"
                     /translation="MPDPSVTDITTPAPPEEKKKDDTEILFETCGLNGIVSLDAFKEA
                     MVGYTRFQPMNSILTIVDFSLPSNEKRFFVIDLANKKLLLSSLVAHGQKSGELMATKF
                     SNKPNSHQSSLGFYRVGAKIFSPKHGDALSLDGLDKGLNDNARAREIIIHGADYVSDD
                     FIKKFGRLGRSFGCPALPRDIMAEVLPIIRDGSILFIYRGTRV"
     gene            complement(1800..4307)
                     /locus_tag="PKV31_05240"
     CDS             complement(1800..4307)
                     /locus_tag="PKV31_05240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNB92296.1"
                     /translation="MQKLIPEYLKGLDATEVEDSRQRFGFNSLRSGSEAAWYDLLVDI
                     FKEPMLILLVAVSIIYLIIGDWGEAAFMFVAIVAVSAISFYQDNRSRKALQELEKLNE
                     PLSTVIRNSEILKIPTREIVVGDLCVVEEGKMVNADGRIVHSNDFSVNEASLTGESMP
                     VFKRADTADSSVYSGTIIVSGLAVFEVEKIGAETRIGRIGTSMKDIGDEASPLQLQIR
                     TFVKNMAIWGIIVFVMICIYRFYQTGDIIESLLSGLTLAMSVLPEEIPVAFTTFMALG
                     AWKLINEGIIVKRSSVVETLGSTTVICTDKTGTITQNSMHLHMMYDFSSGQTYLAHEF
                     SDAALTDLISYAMWASEPVPFDPMEKELHRIYGETANEDLRPQFHMAHEYPLGGIPPM
                     MTHIFENDNGNRIVAAKGAPEAILEVSELDMEQLEDMRAMVRKFSGQGFRVLGVGASD
                     FAASGFPPAQQDLVFRFLGLLIFQDPPKPGIRNVFSKIYQAGIKVKMITGDNTDTAVA
                     IAQQAGIINDAKPVEGQFISSLGEEELRSIVRDTVLFTRMFPDAKLEVVKTLKADGEV
                     VAMLGDGVNDGPALKAAHIGVAMGHKGTEIAKSAASLVITDDDLDKLITGIAAGRRIY
                     TNLKKAVQYIISIHIPIILTVSLPLFLGWVYPAIFTPVHVIFLELIMGPTCSIVYENE
                     PIERHAMSGPPRRMADTFLSWRELAVSILQGLMITGGVLTAYQWMAQNGASEEKTRAM
                     VFATLVLANIFLSFANRSFYYSLFECFMRKNLLFPVITLATLIFLLMILYLPAVSGFF
                     HVTGLTVKEISISVCIAFGSILWFEIYKIGRRVSKKY"
     gene            complement(4390..5247)
                     /locus_tag="PKV31_05245"
     CDS             complement(4390..5247)
                     /locus_tag="PKV31_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB92297.1"
                     /translation="MKHILIPTDFSEPADNAINFAVNSNKYFAESITLLHSLEVGGNM
                     YVDYMGVNKEFNSKMIGEAEARLRNVKQDLAVQYGVAVDTVVSTTGLKEAIDEAVQSL
                     GVDLVVMGTLGASGSLQRIWGSRTSSVIGNTKVPVMTIPVDYTWKKPGKILFTTNFFE
                     KEKAVLDFIFELAELYMAEVQVAVFTNQIDDSAAKFIRHSESLEAYQSFLKTEYREDT
                     LSGAHLYGDDFEETIQKYIEEHEIDILVMVTYQDNIWARFFNPSKTRAMSYQTRVPLL
                     AIPADFGKK"
     gene            5855..>8127
                     /locus_tag="PKV31_05250"
     CDS             5855..>8127
                     /locus_tag="PKV31_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNB92298.1"
                     /translation="MRKLNIFWTFFFALLSFSIHAQIYDPVKWTSSFKQIGDDEYELT
                     WTAKIDNGWSIYSQYIDPNVGPNPTSVVFDNKKAIELIGKNVEGGHIKTVYDEIWEAK
                     ITKFTETAILKQKIKVLDDSKPISGYVNYQTCDDTKCLPPADFEFSFKVKNKKKDQSA
                     ETGVGAVDSKGEASVEVLAGAPNQDDNKEMIQPVSWSATVKKESITKTTIIFNASIEK
                     GYKIYSKDIKGDGPIPTSITLDKNSKGNITGIKEQSTGAKTAFDPYFKINVTTLSKTA
                     SFDVSLDGLEAGKPVSGTIDYMACDDSKCSPFLAYWKTDANLENVVISFEPFPEDAGP
                     AISGSEAGFFPINNIDLNNPLSTCSADSEANVKESTGLFTIFLLGFIGGLIALLTPCV
                     FPMIPLTVSFFTKSNKDKKKGITNAFLYGFFIFLIYILLSVPFHLLDSINPNILNDIS
                     TNAWLNVAFFLIFMVFAFSFFGYYEITLPSSLSNKVSSAEGVGGVIGIFFMALTLALV
                     SFSCTGPILGSLLAGTLSSNGGAWNLTAGMGGFGIALALPFALFAAFPSWMNTIPKSG
                     GWLNTVKVVLGFIEVALAFKFLSNADLVKHWGLLKIEPFLLIWILVALGLFAYLMGWI
                     RFPHDSKLKKLSPTRVVLALISLVFAIYLATGFIYNKETKTFTSLSLLSGLAPPVGYS
                     ILYPNDCPNNLNCFHDLEEGLAYAKSRNLPVFLDFTGYACVNCRKMEEHVWPTNKVKN
                     LLTNEFVVISLYVDDKTP"
BASE COUNT         2384 a         1821 c         1590 g         2332 t
ORIGIN      
        1 ggtcatcggt attcggtccg aatcggatct ccttgataac agtttttact gtctttgcct
       61 tgagctctct ttccttcttc tttttattgt aaagaaactt ctggaagtca ataattttca
      121 caacaggggg agtagcattg ggggatattt ccaccaaatc caaaccggcc tgtctggccc
      181 agtctaaaag caatggcgtg gaataaatcc cggcttctac attatgacct gcgacatcac
      241 ttatcacttc aaaatcttct ccgataagcc ttacctcagg aattctgatt tgttcattga
      301 ttcggaattg agctttttcg gctcctttat cctgtctttc tttttttgcc aaattaaaaa
      361 ttaagattta aaataattat ctgtatcgtt ttgtttgtgg ttgtaacaga gctgattaac
      421 gtaaaacagc ggtattcagt tcctttccgg cacaaatgta atttattttt atttatcagg
      481 tatttatagg tatgggaaaa gtgcatatac cgttgatata ttttaattcc ggggaaagaa
      541 ttgtggcgaa gccaagcctg agcttgataa agagtataag gggtgtatca ggaaggccaa
      601 atgagctaaa aaggaggatg tagtttttaa atctgaattt ttttaacaaa ccatttcaaa
      661 aaaatgacaa tctttgcgtc aactttttca tttttaaaga attgtaaaag gcattcaaga
      721 aactaatcta tgtatttatt cattcggctc ctatctcttt ttttaattgc aacaatgccc
      781 gacccttctg taacagatat cacaacaccc gcccctcctg aagaaaagaa aaaagatgat
      841 acggagattc tttttgaaac ctgcggattg aatggtatag tttcactgga tgcatttaag
      901 gaagcgatgg ttggctatac gaggtttcag ccgatgaatt caattctcac catcgtagat
      961 ttttccctcc cctctaatga aaaaaggttt tttgtcatcg atcttgccaa taaaaaatta
     1021 ttgttgtcat ccctggtcgc tcatggccaa aaatccggtg aattgatggc cactaaattt
     1081 tccaacaaac ccaattctca tcaaagtagc cttggattct accgggtagg agcaaaaata
     1141 ttcagtccca agcatggaga cgctttaagc ctggatgggc tggataaggg tcttaatgac
     1201 aacgcccggg ctagggaaat tataatccac ggggcagatt atgtcagtga tgactttata
     1261 aaaaaatttg gcaggctggg tcgcagtttt ggctgtccgg cattgcccag agatattatg
     1321 gccgaggtac ttcccattat ccgcgatggt tcgatccttt ttatttatcg tggaacaaga
     1381 gtctgatcga atagattttt cgctacagaa gtttttgaaa tattacaaaa aggggattaa
     1441 catagaacgt tcccggatta cgctgcctga tatttacact gacgaagcat agcagtagta
     1501 ataccgaagg aacattcgta aaagaccaat ttcattggcc tgaacgactg taatatcggc
     1561 atccccgagc cccggattgt atcacttcct ggacagagca gagctctggt cgttgtactc
     1621 tcgggacaag catacctaaa actatcggat tattttatca ttcatccggg tataagatca
     1681 atacatctaa tggagaaatg attcacccct ataaaaaacg attttcaaaa actacgcatt
     1741 ccaagctaaa agactcatgc acaggtaaac aatttttttc cttcacccac aacattcttt
     1801 caatacttct tactgactct tctgccaatc ttataaattt caaaccacaa tatcgaacca
     1861 aaggcaatgc atactgatat actgatctcc ttaaccgtta agcctgtcac gtgaaaaaac
     1921 cctgaaaccg caggaagata aagaatcatg agaaggaaga tcagggttgc aagcgtgatc
     1981 acagggaata ggaggttctt cctcataaaa cattcaaaca gactataata aaatgatcga
     2041 ttagcaaagc ttagaaagat atttgctaat accaaagtgg caaacaccat agccctcgtt
     2101 ttctcctctg aagcaccatt ttgagccatc cattgataag ccgtcagaac acctcctgta
     2161 atcatcaaac cttgcaaaat actgacggcc agctcacgcc aactcaggaa ggtatctgcc
     2221 atcctgcgag gcggcccgga catagcatgt cgttctatcg gttcattctc atacacgatg
     2281 gaacaggtag gtcccataat caactccaaa aaaatgacat gtacaggggt aaagatcgcc
     2341 ggataaaccc aacccaggaa caaaggcaag gatacagtca agataatcgg tatgtggatg
     2401 gagataatgt attgaaccgc tttcttgaga ttggtataga tacggcgtcc ggcggcaata
     2461 cctgtgatga gcttatccag gtcatcgtcc gtgataacca aagacgctgc agacttggct
     2521 atttcggtac ctttatggcc catggccaca ccgatatgcg cagccttcaa cgcaggtccg
     2581 tcattgacac catcccccaa catagccact acctccccat cagcctttaa ggtcttgact
     2641 acttcaagtt tggcatcggg aaacattctg gtaaacaata ctgtgtcacg tactatcgac
     2701 ctcaactctt cctctcccag ggaagaaatg aattggccct caacaggttt tgcatcgttt
     2761 atgataccgg cttgttgagc aatagccaca gccgtatcgg tattatcacc ggtaatcatt
     2821 ttaaccttta tgcctgcctg atagatctta ctgaaaacgt tccggatacc gggcttggga
     2881 ggatcctgaa atatcaaaag gccgagaaat cgaaatacca gatcctgttg tgcgggagga
     2941 aatcccgacg ctgcaaaatc agatgcaccg acgcccaaga cccgaaaacc ttgtccacta
     3001 aacttcctaa ccatcgcccg catgtcttcg agctgctcca tatcaagttc ggatacttca
     3061 agaatggcct ccggcgctcc ttttgcggca actattctat tgccattatc attttcgaag
     3121 atatgcgtca tcatgggagg tattccgccc aatggatatt cgtgcgccat atgaaattgt
     3181 ggccttaaat cttcattggc ggtctctcca tagatcctgt gtaattcctt ctccatcgga
     3241 tcaaaaggca caggttcact ggcccacatg gcataagaaa tcaggtcagt caatgcggca
     3301 tccgaaaact catgggccaa ataagtctga ccggatgaaa aatcgtacat catatgcaga
     3361 tgcattgaat tttgcgtgat ggttcctgtc ttatcggtac aaatcacggt tgtgcttccc
     3421 aatgtttcca cgacactaga ccgtttgacg atgatgccct cattgattag tttccaggcg
     3481 cccagggcca taaatgtagt gaatgccacc ggaatctctt ccggcaatac tgacatggca
     3541 agcgtcagac cgctaagtaa gctttctatt atgtcaccgg tctgataaaa ccgataaata
     3601 caaatcatca caaaaacaat gatgccccaa atggccatat tcttcacgaa ggtgcggatc
     3661 tgcaactgaa gtggtgaagc ctcatcccct atatccttca tcgaagtacc aatcctgcct
     3721 atccgtgttt ccgcgccaat tttctccact tcaaataccg caagtccgga aacaataata
     3781 gtgccactat atacgctgga atcagccgtg tctgctcgct tgaataccgg catactctca
     3841 ccggtaagcg atgcttcatt gactgaaaaa tcattactat gaacaatgcg cccatcggca
     3901 ttgaccatct taccttcttc aacgacacac aggtcaccta caacaatctc gcgggtagga
     3961 atcttcaata tctctgagtt tctaattacc gtactcaggg gttcattcaa tttttcaagt
     4021 tcctgtagcg cttttctact cctgttatcc tgataaaacg aaatagctga tacagccact
     4081 atggccacaa acataaatgc agcttcaccc caatcaccaa taatcaggta aataattgaa
     4141 accgcaacca acaaaatcag catcggctct ttgaagatat ccactaaaag atcataccag
     4201 gcagcttcgc tgccactcct gaggctattg aatccaaatc tttggcggga gtcctcaact
     4261 tccgttgcgt ctagaccttt gaggtattcc gggataagtt tctgcatcct actttcattc
     4321 tgctatgact acaagctaca gcctgaaagc gactgtatgc tgtccgatga tttttcatta
     4381 tccggtttac tacttttttc cgaaatctgc cggaatagca agcaggggaa cccttgtctg
     4441 atagctcata gcacgcgtct tactcgggtt gaagaatctt gcccatatat tatcctgata
     4501 agtcaccatg accaagatat caatttcatg ctcttcaata tatttctgga tagtttcttc
     4561 aaaatcatca ccatacagat gggcgccgga taaggtatcc tccctgtatt cagtcttcag
     4621 gaaagactgg tatgcttcaa ggctctcaga atgtctgata aatttagccg ctgaatcgtc
     4681 aatctgattg gtaaagactg ccacctgaac ctccgccata tataattcag ccaactcaaa
     4741 aataaaatca aggacggctt tctccttttc aaaaaaattg gttgtaaaca gaattttgcc
     4801 cggttttttc caggtataat ccaccggaat ggtcataaca ggcacctttg tattgccgat
     4861 gacagaagaa gtcctgctac cccaaatcct ttgtaaagag ccacttgcgc ccaatgtgcc
     4921 catgacaacc aaatcaactc ccagcgactg aactgcctca tcgatggcct ccttcagtcc
     4981 tgttgtagaa actaccgtat ctacagccac accatattgt actgcaaggt cctgcttcac
     5041 gtttcgaagc ctggcttcag cctcaccaat catcttgctg ttaaactcct tattgacacc
     5101 catgtaatct acatacatat tgccacctac ctccaaggaa tgaaggaggg taatactttc
     5161 ggcgaaatat ttattggaat tgactgcaaa gttaatggca ttatcggccg gttccgaaaa
     5221 atctgtggga attaaaatat gtttcatata ctttgatttt atttgtttcg gtcataaaac
     5281 tataatggta gcaatatgaa tgattgaata tctccattaa ggtaaatata taaaaatttc
     5341 cgtaaataaa aaactaataa accgaatagt gcaaaaaaca aattgcattt cttatctata
     5401 tttcactaca atggtgcatg actccgaaaa gaataatcag atagaattgg ttaagcaatt
     5461 agatataacc aatctgatag ttattttcta ttatttactt ttttctcgat aaaaagttac
     5521 aaaaaatcaa ggctgcaccg cattctaaac gcttcttcaa cggtggttta tacaaggccg
     5581 ctctcaacag aatttacaaa gtcgtcaagg actaatcagt attcagtatt gatttgccct
     5641 caaaaaagtc gcaagttttg gttgaaaata cgttaatatt tagtcaaaat aatcaattga
     5701 gccaatgaat gttgctgcaa acatagcccc aggccttgat ttacaagata aaagaagcat
     5761 taattactat atttgccatt ctgaacaaat ctttataaaa agcattatat aatttcgagt
     5821 aaataattaa ctaattcaga caatcctgca tttaatgaga aagttgaaca ttttttggac
     5881 atttttcttt gcacttcttt ctttttcaat tcacgcgcag atttatgacc ctgtcaaatg
     5941 gacgtcttca ttcaagcaga taggcgatga tgaatacgaa cttacctgga cagcaaaaat
     6001 agataatggc tggtctattt acagccagta tatcgacccc aatgtaggtc cgaacccaac
     6061 ctctgttgtt ttcgataata agaaagccat tgagctaatc ggcaaaaacg tcgaaggcgg
     6121 acatatcaag actgtttatg atgaaatctg ggaggcaaaa attaccaagt ttactgaaac
     6181 agctattctg aagcagaaga tcaaggtttt ggatgactcc aagcctattt caggatatgt
     6241 caattatcag acctgcgatg acacaaagtg tctcccacct gcagattttg aattttcctt
     6301 caaggtaaaa aacaaaaaga aggatcagtc ggctgaaaca ggtgtaggtg cggtcgactc
     6361 caagggcgaa gcatcggtgg aggttttggc aggggcaccg aatcaggatg acaataaaga
     6421 gatgattcag ccggtatcct ggtcggcaac cgtcaagaaa gaatcgatta ccaaaacgac
     6481 aataatcttc aatgcctcta ttgaaaaggg atacaagata tattccaagg atatcaaagg
     6541 cgatggtcct atacccacct ccataacgct ggataaaaat tcaaagggca atatcacagg
     6601 gataaaggaa caatcgacag gtgccaagac agcattcgac ccttatttca agattaatgt
     6661 caccacccta tccaaaacag ctagctttga tgtttcactg gatggattag aagcgggaaa
     6721 gccggtatca gggacaattg actacatggc gtgcgatgat tccaaatgct cgccattcct
     6781 ggcatattgg aaaacagatg ccaatctgga aaatgtcgtc atcagttttg aaccatttcc
     6841 tgaagatgcc ggtccggcca tctccggaag tgaagcgggc ttctttccga tcaataatat
     6901 cgacctgaat aatccattat ctacttgttc ggcagactcc gaagccaatg taaaggaaag
     6961 caccggatta tttaccatct ttctgctcgg ctttatagga ggtctgatag cgttgcttac
     7021 cccttgtgtt ttcccgatga ttccccttac tgtaagcttc ttcacgaaga gtaacaagga
     7081 taagaagaaa ggaatcacca atgcttttct atatggattt tttatttttc tgatttatat
     7141 attgttgagt gttccgttcc atttgctgga cagcatcaat ccgaatattc tgaatgacat
     7201 ctcgaccaat gcatggttga atgtggcatt ctttttgata ttcatggtgt tcgcattttc
     7261 attttttgga tattatgaaa tcacgcttcc cagcagtctt tcgaataaag tttcatctgc
     7321 cgaaggtgtt gggggcgtaa tcggaatttt cttcatggct ctgacccttg ccttggtttc
     7381 gttttcctgt accggtccaa tccttggatc tttactggca gggacattaa gttctaatgg
     7441 tggggcgtgg aatctgactg ccgggatggg aggatttggc atcgcactgg cattgccttt
     7501 tgctttattt gcagccttcc ccagttggat gaataccatc ccgaaatccg gtggttggtt
     7561 gaataccgta aaggttgtac tcggctttat tgaggtggcg cttgccttta agttcctttc
     7621 caatgcggat ctggtgaagc actggggctt attgaagatc gagccgttcc tcctcatctg
     7681 gattctggta gcattaggtt tatttgccta cctgatggga tggattcgtt tcccccacga
     7741 cagcaaactt aaaaagttaa gccccacacg agttgtactg gctttgatat cactggtatt
     7801 tgctatttat ttagctaccg gtttcatcta caataaagag accaaaacct tcacttcact
     7861 ttcattactg agcggcttag cgcctccggt aggttacagc atcctgtacc ctaatgattg
     7921 tccaaacaac ctgaattgct tccatgatct ggaggaagga ctggcctatg ccaaatcccg
     7981 caacttaccg gtatttctgg attttacagg atatgcatgt gtcaattgtc gaaagatgga
     8041 agagcatgtc tggccaacca acaaggttaa aaatcttctg accaacgaat ttgtggtgat
     8101 ttcgctttat gtggacgata aaacacc
//
LOCUS       DAOQMC010000098         8114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3559_length_8114_cov_5.060678, whole genome shotgun sequence.
ACCESSION   DAOQMC010000098 DAOQMC010000000
VERSION     DAOQMC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3559_length_8114_cov_5.060678"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKV31_05255"
     CDS             complement(<1..715)
                     /locus_tag="PKV31_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNB92299.1"
                     /translation="MKSLKIVSLLCIALILSCGKDQPVINRGDLEHISYDPHATEYKR
                     PPFYPKMEYPADNPPTLEGIALGRRLFYDPILSGDSTMGCFSCHRQELAFTDGGATSV
                     GIDGINGRRSSMTLVDIGFNYKGMFWDGRAATLEEQALGPVTDPVELHTTWPEVEMKL
                     RRHNAYPQLFRKAFGIRDRKEITKELVAKAIAQFERTIVSSGQAKFDKFRLNQDFLDY
                     DEAIGLDMFFDRQPFVLPDA"
     gene            complement(718..1479)
                     /locus_tag="PKV31_05260"
     CDS             complement(718..1479)
                     /locus_tag="PKV31_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92300.1"
                     /translation="MERSLIIALLMLVLGAASCTREDDTPVGDIRLAFTLHYDGAPIV
                     FNQNCDYNGEFIRMTTFTYFISNIKLWSGSGAVNLKDIEMIDFSKSNVSDQGALEGFV
                     LTIPGVRAGTYDALEFGVGVPKDMNATIPADYPSNNPLSDNGYYWAPWNSYFFSKMEG
                     KFDALSTGTFESGFMFHTGLDENYKVVRFNNPIVINAGNGANVLHVSLDLSKLFVLNG
                     KPINLHEINKAHWPMNKDVIIAITNNYTAAFKLDN"
     gene            complement(1661..2416)
                     /locus_tag="PKV31_05265"
     CDS             complement(1661..2416)
                     /locus_tag="PKV31_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92301.1"
                     /translation="MKNFLIILIGLALFMTSCNKEDAVVNTDLKIVFTLNYDGKPLVF
                     NQNQAYADKFIRMTRFNFFMTNLKLRSGQDSVKLADLSFVDFTLSNVNEGGAKAGLAL
                     TVPSIKSGKYTSLEFGVGLPKDLNATKPIDYPSSNPLSDVTYYWTAWGGYIFSKMEGK
                     FDSLATGLFSEAFTFHTGLDDNYKIVKLVSPINIEGSKTENVLNVSLDVKKLFVMNGQ
                     QIDLKLVNQAHGPSNEEVIRALAQNYQAAFKLE"
     gene            complement(2666..3502)
                     /locus_tag="PKV31_05270"
     CDS             complement(2666..3502)
                     /locus_tag="PKV31_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92302.1"
                     /translation="MRKYWERSKFGIGGIILPLLALMLLSSCGNVKEGCMDANASNFD
                     VSADKDCCCTYPRMGLQFEYYNGDNLLSQAPLIDVDGDTITLSKLTFYLSDLHWKDAE
                     DKEIRSDKRLWLFLSPDNFNDSTRSIDDYYLIEAHNARIQDFTFTRPFSLKSLALTIG
                     VADSALANQPWYMKNANHPLAGKKDNMYDFDAGQYRTLRMAYSIKNSQTDPDTVYVDS
                     GQSRFKTTLTLNYHTVLGFDNYIIIRLYLNRLIEGIRFRTDDPEVISDKLAANLENLL
                     LP"
     gene            complement(3477..4298)
                     /locus_tag="PKV31_05275"
     CDS             complement(3477..4298)
                     /locus_tag="PKV31_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB92303.1"
                     /translation="MNIELKRDLCFFDLETTGLNVIRDRIIQIAIVKISKKDHARTEY
                     YRMVNPGPVFISDEAAAVHGITNEMVRNKPTFPDLAEEIYNFIGDADLGGYNSNRFDV
                     PLLMEEFARAGIDFDMEKRRTVDVQQIFYKMEPRTLKAAYRYYCGKEMSDAHDALADV
                     KATIEVFESQLDFYEGKDLIDEDSGVTKNPIVRDIEALHLFTTNLNTLDATQRLKLNK
                     DGLVVFNFGKYVDQPVVEVFKKERGYFSWMMSKDFSQQVKNIITKIHREEILGKK"
     gene            complement(4295..6109)
                     /locus_tag="PKV31_05280"
     CDS             complement(4295..6109)
                     /locus_tag="PKV31_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNB92304.1"
                     /translation="MEKKEERPKIKFDKSTLNSIVKIMRYMKPYRGYFITGMICLALS
                     SIFFMLFPAMAGEMANAAAGKSKYPFTLSQYGVFFIILLILQSVLSYGRAVTLAVVSE
                     KGVAQVRRDLYAALITQPVAFFEKNKVGELSSRITGDAEQLQNVFSVTLAEFIRQVIT
                     LIVGVAILAYVTPKLSLIMLATFPVIVIAAIFFGKYIRKLSGQRQAEVASSNHAAEES
                     LYAFHVVKAFTNELFEVKRYNNILDHVVDISIRYARVKGLFFVFLITILFGGIFFILW
                     QGALLVQHGEMTIGDLFSFIIYTGIIGGAIASLGTFYTQLAGALGATDRILEIMESTP
                     EIVVPDVLQGDPVSLSGDIVFRDVTFRYPSRPDTNVLKGIDMEIGKGQKVALVGASGA
                     GKSTIVSLLMRFHEVDTGAILINGQDVRGFDLFQYRKNFATVPQDVILFGGSIRENIA
                     YGSPEASDEAIEEAARKANAMEFISRLKDGMDTIVGDRGLRLSGGQKQRIAIARAILK
                     NPGILILDEATSSLDAESESLVQEALHTLMEGRTSIIIAHRLATIVNTDRIFVVNDGR
                     IIETGTHQELMSNPEGWYRYLAGMQFNLTRLSTEQIQS"
     gene            complement(6529..6891)
                     /locus_tag="PKV31_05285"
     CDS             complement(6529..6891)
                     /locus_tag="PKV31_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92305.1"
                     /translation="MAVRKCKLCKKPFEGRTDKIFCGQSCKAVYHQKLKKVTNLVADP
                     IDTILHRNRSTLLEIMGKSASQKKVPRILLDAKKFNWKYITAYHINSRQKMVHYVYDF
                     SWMIFSDQEILIRRLHPS"
     gene            7079..>8114
                     /locus_tag="PKV31_05290"
     CDS             7079..>8114
                     /locus_tag="PKV31_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNB92306.1"
                     /translation="MNDPVELFKVVDSQDKIYFGLKTVKDSREMHLIKSCFGRIWCAE
                     ARIWLLPYGKESWKEISTKLPSIKTNTHHGTIRIRSDVLYHSTSKPEKANTTTKEVKR
                     LSDTHEYAILQMQEQLIVKRYQPATQKNYLSNFRSFLAYFHDVNVREIDKNQIRNFLL
                     HKIKSDKIAESTQNGLINAIKFYYEQVEKRERFTIYDLRPRRPKQMPGFLSKEEVVRL
                     IASIANEKHRLIIKLIYSAGLRLGELIRLKKNDIQVEKNYILIRCSKGKKDRLVTLSE
                     KIKADLVSYIERYKPHYYLIEGQDGGPYSPRSVQNIFANALQKAGIRTHATVHTLRHS
                     YATHLILDGTD"
BASE COUNT         2456 a         1832 c         1603 g         2223 t
ORIGIN      
        1 gagcatccgg cagtacaaat ggttgtctgt cgaagaacat atccaatcct attgcctcat
       61 cataatcaag aaaatcctga ttaagtctaa atttatcaaa tttggcctgg ccgctggaga
      121 ctatagtccg ttcaaattgc gcaatcgctt ttgctaccaa ttccttggtt atttctttgc
      181 gatccctgat gccaaaagct ttcctgaaaa gttggggata tgcattatgt cttctgagct
      241 tcatctctac ttcgggccat gtagtgtgca gttcaaccgg atccgttacc gggcccagag
      301 cttgttcttc gagtgtagct gcccgaccat cccagaacat ccctttatag ttgaatccaa
      361 tatcaacaag tgtcatgctg cttcttcgac cgttgattcc gtctatcccc acgctggttg
      421 cacctccatc agtaaaggcc aactcctgcc tgtgacagga aaaacatccc attgtactat
      481 ctcctgaaag aataggatca taaaataaac ggcggcctaa agcgatgccc tccaatgtag
      541 gcggattgtc ggccggatac tccatcttag ggtagaatgg cggtctcttg tattcggtcg
      601 catgtgggtc ataactgata tgctccaggt caccccggtt gatcacaggt tgatccttac
      661 cacaggatag gataagagct atacaaagaa gagatactat ttttaaactt ttcacgatca
      721 gttatctaat ttaaatgccg ctgtataatt attagtaatg gcaataatga catctttgtt
      781 catcggccag tgagccttgt tgatttcatg aaggttgatc ggtttgccat tcaatacaaa
      841 tagtttgctg agatccagtg aaacatgcaa tacattcgca ccatttccgg cattgatgac
      901 aataggatta ttgaatctta ccaccttata attttcatcc aggcctgtat ggaacataaa
      961 gcctgattca aaggtaccgg tagataatgc atcaaatttg ccttccattt tgctgaagaa
     1021 ataactattc caaggtgccc agtagtagcc attatcggac aatgggttgt tgcttggata
     1081 gtctgcagga attgtcgcat tcatatcttt gggcacaccc acaccaaatt caagggcatc
     1141 ataagtaccg gccctgactc cgggaatagt gagtacgaaa ccctcgagag ctccctgatc
     1201 agaaacattg gatttcgaaa aatcgatcat ctcaatatcc ttcagattga cagcgccgct
     1261 ccctgaccat aatttaatat ttgaaataaa atatgtgaaa gtagtcatcc ggataaattc
     1321 tccattgtaa tcacaattct gattaaatac tataggagct ccatcgtaat gcagcgtgaa
     1381 ggccaacctt atatcaccta caggtgtatc atcttccctg gtacaggaag cagcgcccaa
     1441 tacaagcatc aacaaggcga tgatcaaact tcgttccatt tcattttaat ttattacaaa
     1501 taaaactaaa actaagccaa cgaaataaag ccttacatta aatatttatt aaaaatcaag
     1561 tagatacaca ctgctcccgg ctttattctt tccaatcatt cacgtgcaat caccattgga
     1621 aagaataaat aacaacatca gaaccggacc ggttgggtca ctactccaat ttgaatgcag
     1681 cctgatagtt ttgggcaagc gccctgatca cttcttcatt ggatggacca tgcgcctgat
     1741 tgacaagttt aagatctatt tgctgaccat tcattacaaa gagcttcttc acatcgagtg
     1801 agacattcag cacattttcg gttttactgc cttcgatatt gattggtgac acaagtttga
     1861 caatcttata gttgtcatcc aaaccggtat ggaaggtaaa ggcctctgaa aacaaccctg
     1921 tggccaatga atcgaacttg ccctccatct tgctaaagat ataaccaccc caagcagtcc
     1981 agtaataagt tacatcggag agcggattgg aactcggata atcaatcggt ttggtagcat
     2041 tgaggtcttt tggcaatcca actccaaatt ccagggaagt atatttaccg ctttttatgg
     2101 acggcactgt cagcgcaagt cccgctttag caccaccttc gttgacgttg gagagcgtaa
     2161 aatcaacaaa actcaggtcg gcaagcttaa cagaatcctg tcctgaccgt agtttcagat
     2221 tagtcataaa aaaattgaac ctcgtcattc ggatgaattt gtcagcatat gcctgattct
     2281 ggttaaacac caatggcttt ccatcataat tcaaggtaaa tacaattttc agatctgtat
     2341 ttacaacagc atcctcctta ttacaagagg tcataaacaa agccaaacct attagaataa
     2401 ttaaaaaatt cttcatgtaa aataaaatct ttttaaattg tacaattcaa ttgatataaa
     2461 taatcttcaa aatatcaata attcaatatt ttttaaataa atattatttc gaaaacggta
     2521 gatttttttt gcaaaaatat tgaattatgc ctactgatat tctataataa cgaatatttt
     2581 tgggtaaggt tagcaactaa tgaaaatttc ttttaaaatt ttccattaac aaaaaggttg
     2641 ttcatccaag gttaatttta taaaatcaag gtagtaataa attctccaga tttgctgcca
     2701 atttatctga aataacttcc gggtcatctg tcctgaagcg gattccctct atcagcctgt
     2761 tgagataaag ccggatgatg atatagttat caaaaccgag taccgtgtga taattcaggg
     2821 ttaatgttgt cttaaaccgg gactgaccac tatcaacgta gaccgtatcg gggtctgtct
     2881 ggctgttttt gatgctgtag gccattcgaa gtgtccgata ttgaccggcg tcgaaatcat
     2941 acatgttgtc cttcttgcct gcaagtgggt ggttggcatt cttcatgtac cagggttgat
     3001 ttgcaagggc cgaatcagct acaccaatcg tcaatgccag gctcttgagc gaaaaaggac
     3061 gggtaaaggt aaaatcctgt attcttgcat tatgcgcttc aatcaaataa taatcgtcga
     3121 tgctgcgcgt gctgtcattg aaattatcgg gtgagagaaa aagccacaat cgcttatccg
     3181 accggatttc cttatcttca gcatccttcc aatgcaggtc cgataagtag aaagtcaact
     3241 tgctgagcgt aatcgtatca ccatcaacat caatcagagg cgcctgagac aaaagattat
     3301 cgccattgta gtattcaaac tgaagtccca tcctgggata ggtacaacag caatctttat
     3361 ctgccgatac atcaaaatta gatgcattgg catccatgca gccttccttt acattgccac
     3421 acgaagatag caacatcaaa gccagcaaag gcaaaattat tccccctata ccaaatttac
     3481 ttctttccca atatttcctc acgatggatc ttggttatga tattctttac ttgttgtgaa
     3541 aaatcctttg acatcatcca ggaaaaatac cctctctcct ttttgaagac ttcgacgaca
     3601 ggctgatcaa catacttgcc aaaattgaag acaacgagcc catccttatt taatttgagt
     3661 ctttgcgttg catccagggt gttcagattg gtcgtaaaca ggtgcaacgc ttcgatatca
     3721 cgaacgatcg gattcttggt cacaccgcta tcttcatcaa tcaaatcctt gccctcatag
     3781 aaatcaagct ggctttcaaa cacctctatc gttgccttga catctgcaag tgcatcatga
     3841 gcatcgctca tctccttacc gcaataatac cgatatgcgg cctttaaagt gcggggctcc
     3901 atcttataga aaatctgctg tacatccacc gtccgccttt tttccatatc gaaatcaatc
     3961 cctgcacggg caaattcttc catcaacagc ggcacatcaa agcggttgga attgtatcca
     4021 cctaaatcag catccccgat aaaattgtaa atctcctcgg ccaggtccgg gaaagtcggt
     4081 ttattacgca ccatctcatt ggttatgcca tgcacggcgg ccgcctcatc gctgataaag
     4141 acagggccgg gattgaccat acgataatat tccgtacgtg catgatcttt cttgctgatc
     4201 ttgactatag caatctgaat gattctgtcc cggataacgt taagacctgt cgtctccagg
     4261 tcaaaaaagc agaggtctct ttttaattct atattcatga ttgaatttgt tctgttgata
     4321 atctggtcag gttgaactgc attccggcca aatatctgta ccagccttcc ggattggaca
     4381 tcaactcctg atgtgtgccg gtctcaatga ttctgccgtc attgacgaca aatatacggt
     4441 cagtattgac aattgtggct agtcggtgag caataataat ggaagtcctg ccttccatca
     4501 gcgtatggag cgcttcctga acgagacttt ccgactccgc atcgagggaa gaagtcgctt
     4561 catccaggat caatatcccg ggattcttga ggatagcgcg tgcaatggca attcgttgct
     4621 tctgaccacc ggagagcctc aagccacgat ctccgacaat ggtatccata ccatctttca
     4681 gcctggagat aaactccatc gcattggcct ttcttgcagc ttcctcaatg gcctcatcac
     4741 tcgcttcagg gctgccgtat gcgatgtttt cccgaatgct tcctccaaaa agaatcacat
     4801 cctgaggcac cgtggcaaag ttttttcgat actgaaacag atcgaagccc cggacatcct
     4861 gcccattgat cagaattgcg cccgtatcga cctcatggaa tcgcatcagc agggaaacta
     4921 tggttgactt gcccgcacca cttgccccga ccaaagcgac tttctgtccc ttgccgatct
     4981 ccatgtcgat acctttcaac acgttagtat caggcctgct cggatagcgg aaagtgacat
     5041 ccctgaagac gatgtcacct gaaagggaaa caggatcacc ctgaagcaca tcaggcacaa
     5101 cgatctccgg cgtactctcc ataatctcca ggatacggtc cgtcgcaccc aaggctccgg
     5161 ccaattgagt atagaaggta cccagacttg cgatagcacc accaatgata cccgtatata
     5221 tgatgaatga aaaaagatcg cctatggtca tctccccgtg ttgcaccagc aaagcaccct
     5281 gccacaggat aaagaagatg ccgccgaaca ggatagtaat caaaaaaaca aaaaacaagc
     5341 ctttgaccct ggcatatcgg atggaaatat ctacaacatg gtccaggata ttattgtacc
     5401 tttttacttc aaataattcg ttggtaaatg ccttcacgac atgaaacgca tacagagatt
     5461 cctccgcggc atgattggaa gaagcgacct cagcctgtcg ctgcccggac agtttgcgga
     5521 tatattttcc gaaaaatatg gcagcaatga cgattaccgg aaaggtagcc agcataatca
     5581 gactcaactt cggtgtgacg taagcaagga tagcaactcc gacgatcagg gtaatcacct
     5641 gtcggataaa ttccgccaaa gtgacactga atacattctg caattgctca gcatcgccgg
     5701 taatgcgtga actcaactct ccaactttat ttttctcaaa aaatgctacc ggttgtgtaa
     5761 tcaaggctgc ataaagatct cgcctgactt gagcaacacc cttctcactc actacagcca
     5821 gggtgaccgc acgaccatag gacaagacac tctgcaaaat caacaggatg ataaagaaca
     5881 cgccgtattg gctcagcgta aaaggatact tgcttttgcc ggctgcggca ttggccatct
     5941 cccccgccat tgccggaaac aacatgaaga aaatacttga caaagccaga catatcatcc
     6001 cggttatgaa gtatccccga taaggcttca tgtatcgcat gatcttgaca atactgttca
     6061 aggtagactt gtcgaacttg attttcgggc gctcctcttt cttctccacg actataaaat
     6121 taggttgcaa aggtagcgag aaaggaagga aaaaaagtta atgaagtgaa aaagaagaaa
     6181 atgcccctta acagctaaac aacaaagagc agaaaggcga ttgaaaatgg gtagcatcta
     6241 aaatagactt actctaatat tccgtaacaa atattctgcc gggcgaatcg ctgtaatcga
     6301 ttgaatcatg agaaaccaca tgattgatag taaaacagga acaatatgag agcaataatt
     6361 taaaagatga cgaggtgtta tactgaatac tcagccatgg tcagcatggt gatccaaatg
     6421 gcgttttagg catgattttt tttatctaaa attactaacc atgaagctga ttatggggaa
     6481 gatcggggca agttctcagt ccggatgaac ccacagcttg ttctgcaatc aggatggatg
     6541 cagtcttctt atgaggatct cctgatcgga aaaaatcatc caggagaaat catagacata
     6601 gtgaaccatt ttttgccggc tgttgatgtg ataggcagtg atgtatttcc agttaaattt
     6661 tttggcatcg agcaggatgc gggggacttt tttctgtgaa gcagattttc ccataatttc
     6721 gaggagggta gatcggtttc ggtggaggat ggtatctata ggatcagcaa ccaggtttgt
     6781 tacctttttt agtttttggt ggtaaacggc tttgcagctt tgtccacaaa atattttatc
     6841 cgttctgcct tcgaaaggct ttttacacaa tttgcatttt cgtactgcca tagttgacgt
     6901 tattttacag aataccggga attagtatgg aggactctcc ctaaaataaa agtaattaac
     6961 ggtaaactta cggctatttt tttacataag agtaagttta cggttaaatt acgattaata
     7021 tcgtataagt gactaaataa caggcatata attctatttt tggtgtaaaa aaacaactat
     7081 gaatgaccct gttgaactct tcaaagtagt ggacagccaa gataagattt attttggatt
     7141 gaaaacagtc aaggatagtc gggaaatgca tctgattaag tcctgctttg ggagaatctg
     7201 gtgcgcagaa gccaggattt ggttacttcc ttatggaaaa gaaagctgga aggaaatcag
     7261 caccaaacta ccttccatca aaacaaatac ccatcacgga actatccgta ttcgttcaga
     7321 cgtcctctat catagtacaa gcaaacctga aaaggcaaac actacgacca aagaggtaaa
     7381 aagattgagt gatacccatg agtatgcaat attacaaatg caggaacaac tcatagtcaa
     7441 gcgttaccaa ccggcaacac agaaaaacta tctgagcaat ttcagatcat ttttagcata
     7501 ctttcatgat gtgaatgttc gggaaataga caaaaaccaa attcgcaatt ttctattaca
     7561 caaaatcaaa tcggacaaaa tagcagaatc cacccagaac gggcttatca atgctataaa
     7621 gttctactat gagcaagtag aaaaaaggga gcgtttcacc atttatgatc tccgtccacg
     7681 acgtccaaaa caaatgcccg gatttctgag caaggaagaa gtagttagac tgattgcatc
     7741 cattgccaat gaaaagcacc gcctcatcat caaactcatt tactcagccg gattacgact
     7801 gggagagctt atccgtttaa aaaagaatga catacaggta gaaaagaatt acatcctcat
     7861 cagatgctct aaaggaaaaa aagatcgctt ggtgacctta tcggaaaaaa tcaaggcaga
     7921 tctggtttca tacattgaac gctacaagcc tcattactac cttatagaag gacaggacgg
     7981 aggcccatat tcgccgcgga gtgttcaaaa catctttgca aatgcccttc agaaagccgg
     8041 aatccggacg catgccaccg tccatacact cagacacagc tatgccactc atctgatctt
     8101 agatggcaca gata
//
LOCUS       DAOQMC010000099         8109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3563_length_8109_cov_4.581326, whole genome shotgun sequence.
ACCESSION   DAOQMC010000099 DAOQMC010000000
VERSION     DAOQMC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3563_length_8109_cov_4.581326"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..2095)
                     /locus_tag="PKV31_05295"
     CDS             complement(206..2095)
                     /locus_tag="PKV31_05295"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNB92307.1"
                     /translation="MNLRTGLIFVFLAFYLARANAIQGVLPEYERILSFRSEIVIDSS
                     GKLIVEETILVNALNISINRGIFRALPTERNVNDHTIQVSYQIISIKKNGVEEPYHDQ
                     YENGYKIIYIGDEDIYLSPGIYQYKITYSTERQIGFFDHYDELYWNITGNKWAFPIDT
                     AIATVTLPEAAQILQSSCYTGSYGSTSRNCNAEQKTPNSMTWTAFNLGTNEGLTVAVG
                     FTKDIVSRPVIPDSMKPIALMKWFLYVGLFLFVIMAWLWYRHGVDLPAPAVYPQFDTP
                     EGLSPAAIGYLEGGKYRNTLMAASLINLAIKKLIKIEEVPKSGFFGRSKYIITRLDTQ
                     DVPLSTEESNVLYNMVGYKGSTLELDGTYDSKVQQTVQSFKDSIKQQYNSIIKEGSNR
                     KYVFFVFLFISLAYLPVLNLIHKTYYYAGEQSNGAGFFVVYIILYFIAHFSGYRTSRW
                     IWLIPLLLTIALFYFILTSRHYINSYWVLILFYGMIMTGISFFSYAIRQPGKRFLELQ
                     SLIAGFRMYLGAAENQLIKFHNPPKMTPEVFEKYLPYALVLGVDNIWGRKFETALKEQ
                     GKEYQNQWYVGPNSFSNSFAGSFSQNLSSRMTSASTQPSSSSSGSGGGGFSGGGGGGG
                     GGGGW"
     gene            complement(2100..2654)
                     /locus_tag="PKV31_05300"
     CDS             complement(2100..2654)
                     /locus_tag="PKV31_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNB92308.1"
                     /translation="MKFLLFFFLLTVVILFFFISIYNKLVRAKNLMLEGWSGIDVFLK
                     KRHDLIPNLVETVKGYATHERETLDSVVQARAQALNAGSISEKQAAENNLGKSLVNLF
                     AVAEQYPDLKANANFQALQGQLVNIEGDIEKARRYYNGTARDYNIAIESFPGNILAGA
                     FTFGPASYFEIEDSGERSVPQVKF"
     gene            complement(2967..3188)
                     /locus_tag="PKV31_05305"
     CDS             complement(2967..3188)
                     /locus_tag="PKV31_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HNB92309.1"
                     /translation="MEELNMKDVLEKSAFGVCTYIGEKVGIATSRVRLYFIYLSCATL
                     GSSMILYLFMAFWVNIRKSLKRNDRTAWE"
     gene            4029..4111
                     /locus_tag="PKV31_05310"
     tRNA            4029..4111
                     /locus_tag="PKV31_05310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4063..4065,aa:Leu,seq:gag)
     gene            complement(4192..6309)
                     /locus_tag="PKV31_05315"
     CDS             complement(4192..6309)
                     /locus_tag="PKV31_05315"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNB92310.1"
                     /translation="MKYFASLLGILFLFLFSTTGFSQDDLPRIELVKVFSGLSKPVSL
                     QSCNDNRIFIVEKGGKIKIGFPDGSVLDSNFLNISDKVLSGGERGLLGLAFPPDYKTS
                     GMFYVNYTEKDEGNTIIARYRISSDSNRAVKASEEILLEIDQPFANHNGGNIEFGHDG
                     YLYIGMGDGGSGGHPQDNSQNKQKLLGKMLRIDVSSSPGYDIPADNPFVGDSEYRPEI
                     WSLGLRNPWRFKFDELTGDLWIADVGQNKWEEIDFEEAGDGGRNYGWRCYEGYVPYNT
                     AGCGDILGYTFPVAAFSHDEGHCSISGGVVNRKQPNSSLYGYYYSVDYCSGVFWGTKR
                     NEDKSFTTIELARTNIDGFVAFGYDKDKNIYAARDNGQIYRIDTFLLCAPELVIEGTD
                     TEVGCKIDSVVLSTQEISNGQYTWFLDDEPINGSNASTIVVTKPGKYSVQFDSEECSA
                     TSNVDYVLEQNKEVDVTILDVPENYCFNGAPLVFHGQPAGGGFFGTGMVDSVFYPSIS
                     DIGTFFITYSYEDEEGCNGFDVGVITVNHPPGTKILSTPPQLCQFATPYVLEATPAEG
                     SWSGPGLTGSTFYPYLAGVGVHKIKYTYNPWPGCFAYDSIYITVIDCTNGVGEADSPE
                     ISISPNPVKDKVVVALKNRNYNPENISIINSLGKEVIRKKLDTHNSAEKLVELSVEGL
                     TPGVYHVIINVGEKRLQRKIIKI"
     gene            6815..7921
                     /locus_tag="PKV31_05320"
     CDS             6815..7921
                     /locus_tag="PKV31_05320"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNB92311.1"
                     /translation="MNINQPEKMTLLEKIKVKRPFIIAGPCSAETEQQVIDTAVNLSR
                     DGNVDVLRAGIWKPRTRPGSFEGVGTVGLPWLQKAKEITGLPVAIEVANAKQAEEALD
                     HEIDIVWIGARSTTNPFSVQEIANVLKGVDIPVWIKNPINPDLELWTGAVERIQQSGI
                     RTIGLIHRGFHSYGDNEYRNTPMWNLAIEMRRRYPELLFINDPSHICGNRHMLAEVAQ
                     TAVDLAYDGLMIECHIDPDHAWSDSAQQITPDGLSKLLKKIHWVQFIENGETDEQLIR
                     LRHQIDHMDDQIIELLSQRMVISDAIGAYKKEHNLSILQMKRWNEIMDRISRKGKDLM
                     LSNEFIHQYFNAIHMESIRRQNKVINKKIDHLNN"
BASE COUNT         2554 a         1796 c         1486 g         2273 t
ORIGIN      
        1 tatcgatgaa tgccagaaag gggtttaact tattatttct tttgtgcata atgaattgtt
       61 ttaatacacg actgatacaa aaccattaca tgagtaagaa tcaatcgaac catgcaaacc
      121 aatttttccg ccaattataa tccaaagtaa aataaaaaaa ttggttttcc caatattaaa
      181 accaattttt ccaaatatta tagggtcacc agccaccacc gccaccaccg ccgccaccgc
      241 cgccggagaa gcccccacct cccgatccgc tggatgaaga acttggctgg gtactggcag
      301 acgtcatcct gcttgaaaga ttctgactaa aactgccggc aaaactattg gagaatgaat
      361 ttggaccaac ataccattga ttctgatact cctttccctg ctctttgagt gcagtctcga
      421 acttcctgcc ccagatatta tcgacaccca gaactaaagc atagggaaga tacttctcaa
      481 aaacttcagg cgtcatcttc ggaggattat gaaatttgat gagttgattc tctgcggcac
      541 caagatacat cctgaaaccg gcaatcaagg attgaagttc aaggaacctc ttaccgggtt
      601 gacgaatggc ataactaaaa aaggaaattc ccgtcataat catcccataa aataaaatca
      661 atacccaata agaattgata taatgccttg atgtcaggat aaaataaaat aatgcaatag
      721 tcaacaacaa aggtattagc caaatccatc gcgatgtgcg atatcccgag aaatgggcaa
      781 tgaagtaaag gataatatat actacgaaaa aaccagcacc attactttgt tctccggcat
      841 aataataagt tttgtggata agattcaaaa ccgggaggta cgcaagacta ataaacagaa
      901 agacaaagaa cacgtatttc ctgttggatc cttctttgat aatcgaattg tattgctgtt
      961 tgattgaatc cttgaatgac tgtactgttt gctgtacttt ggaatcatag gttccatcga
     1021 gttccaatgt cgagcccttg tatcccacca tgttatacag aacattcgat tcttctgtac
     1081 tcaaaggaac atcctgcgtg tccaatcttg tgataatgta tttacttcta ccgaaaaagc
     1141 ccgatttggg aacttcttcg attttaatca actttttaat ggcaagattg atcagagaag
     1201 cagccatcaa ggtatttctg tattttccgc cctcaagata gccaatcgct gccggactca
     1261 ggccttcagg agtatcaaac tggggataga ctgcaggagc aggcaaatct acaccatgtc
     1321 ggtaccacaa ccaagccatg ataacaaaca agaacagacc gacatacaaa aaccacttca
     1381 tcaatgcaat gggtttcatg ctgtccggga taacaggtct ggatacaata tccttagtga
     1441 agccaacagc aactgtcagt ccttcatttg ttccaaggtt gaaggctgtc catgtcatag
     1501 aattgggagt tttttgttct gcattgcaat tcctggaagt actgccataa gatcctgtat
     1561 aacaggatga ttggagaatc tgtgccgctt cgggaagcgt aactgttgca atagcagtat
     1621 caatcggaaa tgcccattta ttgccggtta tgttccaata caattcgtca taatggtcaa
     1681 aaaaaccgat ttgtctctca gtactatagg ttatcttata ttgataaata ccaggactta
     1741 agtagatatc ctcatcgccg atataaataa tcttgtaacc gttttcatac tgatcgtgat
     1801 aaggctcttc aacaccgttt ttctttatgg agatgatctg ataacttact tgtatggtat
     1861 ggtcattgac attccgctca gtgggaaggg ccctgaatat accccgattg atgcttatat
     1921 ttaaagcatt gacaagaatc gtttcctcta caatcaattt tccggaagaa tctatgacaa
     1981 tctccgacct gaagcttaaa atacgctcat attccggcaa gacaccttga atagcattag
     2041 ctctggcaag ataaaatgcc agaaagacaa atatcaatcc tgtccggaga ttcataggat
     2101 caaaacttaa cctgcggtac gcttctttct cctgaatctt caatctcaaa ataagaggcc
     2161 gggccaaatg tgaaagctcc tgcaagaata ttccccggaa aggattcaat agcgatgttg
     2221 taatcgcgtg ctgtaccatt gtagtatcgc cttgccttct caatatcacc ttcaatattg
     2281 actaactgcc cttgcagggc ctgaaagttg gcattggctt tcaggtccgg atattgttcc
     2341 gctacagcaa aaaggttaac aagagacttt cccaggttat tctctgccgc ctgcttttca
     2401 cttatggatc ctgcattaag tgcctgtgct ctggcctgca ccacggaatc cagtgtttcc
     2461 cgctcatggg tagcatatcc cttgaccgtc tcaacaagat tgggaatcag atcgtgcctc
     2521 tttttcaaaa acacatcgat gccgctccaa ccttccagca ttaggttctt ggcacgcacc
     2581 aacttgttat agatactgat gaagaaaaag agtatcacaa cggtaagtag gaaaaaaaag
     2641 agtagaaatt tcataacact aaagtttaag caaccaaata taaacaaaaa tcgtgtaatt
     2701 ctcggtttta tacattggaa tatcattttt ccgtgcttac aagctttaaa ggacttagat
     2761 tgcctgataa agctccggat gtgccgagta aaaatttttg aaatgggtct tggataagaa
     2821 agattcaagt cggctacctt accgggctaa atggcaggta aaataattac aaaaagggag
     2881 aaatgcgggg catttaatca tagggaataa aaccgccata tacaggtaat ttcaagattc
     2941 cttatcgtat aattcaaaat agcaacctat tcccaggctg ttctgtcatt tctttttaag
     3001 gacttgcgta tattgaccca aaacgccatg aaaagatata gaatcatgga ggaacccagg
     3061 gtagcgcaac taagataaat aaaatatagt cgaacgcgtg aagtagctat ccctactttc
     3121 tctccgatat aagtacagac accaaatgcc gacttctcaa ggacatcctt catgttcaat
     3181 tcttccattg ctatgtattt aatccatgca tagaaactac aaatttacca taaatcacag
     3241 aatttccaat aaataacgca gattgtgtgg ataaaaaatg tactttttac tcacgtttag
     3301 atgtaaagat gcctggaccc taaaagcaaa atcaaatagt atcagatatt caattggtca
     3361 taaccaatcg tagagtacca tattgcaatt tattttttct tgctataaaa ataatgccat
     3421 atgacattcc caatagtcca atttcatcga cccaaacgac tgctacaacg gcatctccgg
     3481 accccggatt gtttcacccc cccggacaga gcaaagttct ggcgcttagc tctcgggaca
     3541 agtatccaag tattttttat actgtttcat gattctcctt ggtataacaa gaatcaaggc
     3601 catgtagaat actttgtgct tttatgaaaa taaatcattc aaaaaaaact taacgcactg
     3661 tatatatgac tccacgctca cttcagggta tatccttttg ttattttttt gaccatttac
     3721 ctacgcatcc gttaccgcag gaaccaatta tggtagggtt tattttacct ttagttcaat
     3781 ctccggtgaa attgtaggct tgttataagg ccggatccat gcagaactta ctttcggaca
     3841 atgcttgatt tttatagttg tgcaagatat cgtacacatc tttttaattt aattatggtt
     3901 gaaataaaat agattttcta tcttcacgcc cgtttttgaa taaatcattt caaaaaccta
     3961 ccggaaaaag tcaccggcag aaatacgata aatagcatcc tattatcgtg aaacattgac
     4021 accatcacgc ccacgtgatg aaattggtag acatgcaatc ttgagggggt tgtggactat
     4081 gtccgtgccg gttcgaatcc ggccgtgggc actacttaga aaagaaattc cataaaacaa
     4141 aacaggcccg gaaagattaa accggacctg ttttgtttta tggaatacag attaaatctt
     4201 gattatcttt ctctgcagcc tcttttctcc tacatttatg atgacatgat agactccggg
     4261 cgtcaatcct tctacggata actctacaag tttttcagca gaattatggg tatcaagttt
     4321 tttcctgata acctcctttc ccagactatt gatgattgat atattttccg gattgtaatt
     4381 tctatttttc aaggctacca ccaccttgtc cttaacagga ttgggcgaaa tactgatctc
     4441 gggtgaatca gcctctccca ctccattcgt gcaatcaatg actgtgatat agatactatc
     4501 ataagcaaaa caaccgggcc atggattgta ggtatacttg attttatgca caccgacacc
     4561 tgccagataa ggatagaatg tgcttccggt taagcccggc ccactccacg aaccttctgc
     4621 cggagtagcc tccagtacat aaggcgttgc aaactgacat aattgaggtg gggtagacag
     4681 gatcttcgta ccgggcggat ggtttaccgt aatcacaccc acatcaaaac cattgcagcc
     4741 ctcctcatcc tcataggaat aggttatgaa aaaggtcccg atatcgctga tggaaggata
     4801 aaacaccgaa tcaaccatac ccgtaccaaa aaatccgcca cctgcaggtt ggccatgaaa
     4861 tacaagtggc gcaccattaa aacaataatt ctccggcaca tcaagaatcg tcacatcaac
     4921 ttccttgttt tgctcgagca cataatctac atttgatgta gcactgcatt cctccgaatc
     4981 gaattgtacc gaatatttgc ctggtttagt aacaacaatc gtacttgcgt tgcttccatt
     5041 gataggctca tcatccaaaa accaggtgta ctgcccattg ctaatctctt gtgtggaaag
     5101 aacgacggaa tcaatcttac atccaacttc agtgtcagtg ccctcaatga caagttccgg
     5161 ggcacacagt aagaaagtat cgattcggta gatctgtcca ttatctcttg ctgcataaat
     5221 attcttatcc ttatcataac caaatgccac aaagccatcg atattggtcc gggccagttc
     5281 tatggtcgta aaggatttat cctcattgcg cttggtcccc cagaatactc cggaacagta
     5341 atctaccgag taataataac catacaagga actattcggt tgcttcctgt tcacaacccc
     5401 acctgagatg ctacaatgac cttcatcatg actaaatgct gctacaggaa aagtatagcc
     5461 taatatatca ccacaaccag ccgtattata aggcacataa ccctcataac accgccatcc
     5521 gtagttacgt cctccatccc cggcttcttc aaagtctatc tcctcccact tgttttgccc
     5581 gacatcggcg atccaaaggt cgcctgtcaa ttcatcaaat ttgaatctcc acggattcct
     5641 cagacccaat gaccagatct ccggcctata ttccgaatct cctacaaacg gattgtcggc
     5701 gggaatatca tatcccggac tgctactcac gtcaatccgc aacattttgc ctagcaattt
     5761 ctgcttattc tgagagttgt cctgtggatg accgccactt cccccatcac ccataccaat
     5821 gtacaaatac ccatcatgac caaactcaat atttcctcca ttatgattgg caaatggttg
     5881 atcaatctcc aggagaattt cctctgaggc tttgaccgcc ctattggaat cagatgaaat
     5941 acggtaccgg gcgatgatgg tgttaccttc atccttctcg gtataattga catagaacat
     6001 gccactcgtc ttgtagtccg gtggaaaggc aagaccaagc agcccacgct caccacctga
     6061 caacacctta tctgaaatgt ttagaaagtt agaatccagc actgaaccat caggaaagcc
     6121 aatctttatt ttgccgccct tctctacaat aaaaatcctg ttatcattgc agctctggag
     6181 ggatacaggt tttgaaagcc ctgaaaatac tttaaccaat tcaatacgtg gcaaatcatc
     6241 ctgagaaaat ccggtcgtac tgaataagaa caaaaaaaga attcctaata gactagcaaa
     6301 atatttcata tttttctgag tttaaagctt taaaaatatt tcattttttc aaattttcac
     6361 aatttattac tcattttgag tgcaaaattt aggtaacata tatttactca ttttatataa
     6421 aactttaaat caaacgttta aatatcacat attttaacca accgttgatt aacaccctgc
     6481 acccaatacc attgtcatga tttacaatga attagtgtag ttattattga actttaggtg
     6541 atgattcatc ctgttaactc tcttttgtca accggctttt tctgccaaaa atcccaatcc
     6601 gcctcgcttg atgtccaaaa tgaatatcat ccctattaaa ccctccatca ttcatccttt
     6661 attaaaaatt atagcagaat atttcagcgg atgataactt gactttggag agtatccttg
     6721 acaactccac tcaaactaaa aaattcatgc gaatccttta accttaagta cctttgttcc
     6781 atttaaatat taactccacc gctaaggtta agtaatgaat ataaaccagc ctgagaagat
     6841 gacgctcctc gaaaaaatca aggtgaaaag accttttatt atagcaggcc cttgtagtgc
     6901 cgaaactgaa caacaggtca ttgacactgc ggtaaatcta agccgggatg gcaacgtcga
     6961 cgtattaagg gcaggaatat ggaagcccag gacaaggccc ggcagctttg aaggagttgg
     7021 aactgttggt ttaccatggt tacagaaggc aaaagaaatt acaggtttgc cggtggcaat
     7081 agaggtggca aatgcaaagc aggcagaaga ggcacttgac catgaaatag atattgtgtg
     7141 gatcggagcc agaagtacga ccaatccttt cagtgtccag gaaattgcca atgtcctgaa
     7201 aggagtcgac attcctgtct ggatcaagaa tcctatcaat ccggacctgg aactctggac
     7261 cggcgccgta gaacggatac aacaatcggg tattcggaca atagggctta tacaccgagg
     7321 tttccattct tatggagaca atgaataccg gaatacacct atgtggaatc tggcaattga
     7381 aatgagaagg cgataccctg aattgctttt catcaatgat ccgtcacaca tctgcggcaa
     7441 cagacatatg ctggccgaag tggcccagac tgccgttgat ctggcttatg atggcttgat
     7501 gatagaatgt catatagacc ccgatcatgc atggagtgat tcagctcaac aaatcacgcc
     7561 tgatggcctg agcaaattat tgaaaaaaat ccactgggta cagtttattg aaaatggaga
     7621 gacagacgag caattgatta gacttcgtca ccagatcgac catatggatg atcaaatcat
     7681 agagttgctt agccaaagaa tggtgatctc ggatgcaata ggcgcgtaca agaaagaaca
     7741 taacttatcc attctccaaa tgaaaagatg gaatgaaatc atggaccgaa tcagcaggaa
     7801 aggaaaagat ctcatgctga gcaatgaatt catccatcaa tatttcaacg ccatacatat
     7861 ggaaagcatc cggcgtcaaa acaaggtgat taataaaaaa attgaccatc ttaacaatta
     7921 aaaggaaact aattccggct ctctgcacat tgactaaata atcaggggcg taaaaccaaa
     7981 cccgaaaatt tcagaatgaa ttgaccaatc aattattttt aaggcaaatt tttgaagtaa
     8041 atattacata ttatatacaa ttatttatct ttgattatta atattatata aatgcataga
     8101 atattcaat
//
LOCUS       DAOQMC010000100         8079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3585_length_8079_cov_4.405035, whole genome shotgun sequence.
ACCESSION   DAOQMC010000100 DAOQMC010000000
VERSION     DAOQMC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3585_length_8079_cov_4.405035"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..1317)
                     /locus_tag="PKV31_05325"
     CDS             complement(388..1317)
                     /locus_tag="PKV31_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92312.1"
                     /translation="MAEKVNRDKWLFLICVIVAIVFWAMQKLSNNYSFTQVYSVSYII
                     PDGKVFKKPPVTSVEVTLTGIGWDIVNKSAKNIGDKIEVELSADNSQLIPATAISEKI
                     NSLLASDEISVAQIKPSNISISLVDQAKKKVPVVSKLNIEFLPSFYIKSPIHFTPDSV
                     VITGSYQAIKNITKWNTEILNINKLNRNIYDMPIQLEAPKTDLIRLDHDMVRMNLEVE
                     EFTEKSFTLPIKIKNADNKVISIPNTVDVKCVVGISKYDILTEDDIELYINLDESLVS
                     NSINVVPISVSRMPFYVRNISYQPSSAQFFYLK"
     gene            complement(1324..1620)
                     /gene="yajC"
                     /locus_tag="PKV31_05330"
     CDS             complement(1324..1620)
                     /gene="yajC"
                     /locus_tag="PKV31_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNB92313.1"
                     /translation="MEGLYSPLLLLGMVAVMYFFMLRPQIKKQKEQQKFGEALAKGND
                     VVTSSGIIGRISKIEGNIVTLQVDPKTYIKVTKGSISKELTEAMGSSIQDSINN"
     gene            complement(1642..2127)
                     /locus_tag="PKV31_05335"
     CDS             complement(1642..2127)
                     /locus_tag="PKV31_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNB92314.1"
                     /translation="MMKLIWILILGLGILTVGCKRDVESDEKKVEQILGDGNDYQELI
                     RNPITADENMDTVNIAVAKFDEVSHDFGDIKAGDIVTHTFHFVNAGKVPLLIKDATSS
                     CGCTVPEIPKQPIAPGEKGEILVRFNSENKSGYQSKVVTVFTNGYPSKYLLTIKTNIL
                     K"
     gene            complement(2293..2883)
                     /locus_tag="PKV31_05340"
     CDS             complement(2293..2883)
                     /locus_tag="PKV31_05340"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNB92315.1"
                     /translation="MIIESQYFPPISVLAAFKRRGKVIIDTGDFFRKGSFRNKCYINA
                     SQGPLLLSVPLQKGKNNLQPFSDVRISYDEDWVSQHLSSLQTCYGKSAYFIYFFDEIT
                     ALLKERPVYLLDLNRNILKMIATYLIPEVTIIYSDSYVDEYLGEDKRNQILLKNYTLF
                     ESPRYLQVFEDRTGFVPNLSCLDLLFCLGKYGHDIL"
     gene            2996..4321
                     /locus_tag="PKV31_05345"
     CDS             2996..4321
                     /locus_tag="PKV31_05345"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNB92316.1"
                     /translation="MLNTFSDAEAFLYSSLPMFQRQGPAAYKADIGNISRLMETVGNP
                     HSCGIRMIHIAGTNGKGSVTHLTAAYLQACGYRVGVYTSPHYTDIRERIKINGTLIGE
                     DDFVICLQAFLPFIEEIKPSFFEIICAMAFLYFRDQQVDFAVIETGLGGRLDSTNIIF
                     PILSVITNISPDHQSMLGDTLEKIAYEKAGIIKKNTPVVIGQKISETIQVFQDKAASE
                     DASISFAEDNWQCIYKYNDSHYTHLQIRDRISNTSATHKTALMGIYQTQNIITFLEIC
                     RQLSLYNNIPFNWEYISIALKDIVKLTYYIGRWQIVSQKPLIILESAHNVAGIEAVRK
                     QLSGLEVGQVTIVFGTVSDKDVVPVLKLLPKKARYIWTKADIPRAMDEVELSEKAAFI
                     GLAGMTSPTVKDALQLALDHTSEDDLILVTGSIFVVGDAMTGLKEKGIL"
     gene            4868..5824
                     /gene="metF"
                     /locus_tag="PKV31_05350"
     CDS             4868..5824
                     /gene="metF"
                     /locus_tag="PKV31_05350"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNB92317.1"
                     /translation="MKVTDYIHEAGGKTLISFEILPPLKGGSIQTIFKAMDPLMEFKP
                     PFIDVTYHREEFLYQMQPTGYYQKTSIRKRPGTVGICAAIMHRYGVEAVPHLICGGFN
                     REDTENALIDLHFLGIQNVLALRGDARRFEDKFSPEPDGHHYAVDLIHQITGMNNGSY
                     LDTNIEKGSRTDFCIGVAGYPEKHFESPNLELDIKHLKAKIDAGGNYIVTQMFFDNKV
                     YFDFVKLCRENGITVPIIPGLKPLTKLYQLNSIPRNFYVNLPNDLVREAQKAKTDEDL
                     KQIGIEWCIAQSRELVENNVPCLHFYTMSDAQVVKKICSKIV"
     gene            5830..7065
                     /gene="fahA"
                     /locus_tag="PKV31_05355"
     CDS             5830..7065
                     /gene="fahA"
                     /locus_tag="PKV31_05355"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNB92318.1"
                     /translation="MKSWLEVISDSDFSIYNIPFGIAEIGGEKLIVTRLGDKVIVLDM
                     LQDAGVFAELDLPYGIFVADFLNDMIGLGKPITTSIRSAIQKYFTDEAYAQQFGLDAG
                     QYLIDYQLVKMAMPIRVGDYTDFYSSREHATNVGIMFRGPDNALMPNWLHLPVGYHGR
                     SSSIVVSGTDFKRPAGQMLDKDTGKPFYGKSHELDIEVEMAFIVGRNTHLGETIDVNE
                     AEDFIFGMVLFNDWSARDIQRWEYVPLGPFLGKNFASTISPWVVTMDALEPFRTAGPV
                     QDPEVLNYLATRGSKSFDIPISATLTTKEGVETVICETNYKFLYWNPSQQLAHHTVNG
                     CNVNIGDLMASGTISGSDPSSYGSMLELAWKGTKPIQLADGSERTFLIDGDSITLRAK
                     AVSGEVVVGFGEAKGTVLP"
     gene            complement(7103..>8079)
                     /locus_tag="PKV31_05360"
     CDS             complement(7103..>8079)
                     /locus_tag="PKV31_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB92319.1"
                     /translation="VHPGFSLGYIVRRSNPASITEYPDTQILRSTEALIAKPDIDLIV
                     VNTPVQTHYEIAKQALATGKHVLVEKPFTVTYRQALELIDIAREHNAKLSVFQNRRFD
                     RDYLRVKTILESGNLGQIHEATIRFDRYRTGSSGKLHKEGNFPGSGALYDLGSHLIDQ
                     ALQLFGPPENIYADLMQLRPDVESDDYFELLLYYQQLRVRLISNVMVTLPEPGYVLNG
                     ELGSLSQLRSDMQEKELGVGICPSSENWQKPLSEPDSILTYHEQNNIKRKESFSDPGN
                     YFWFFDALHQFLNGRGPNPVSPEQAAEVIRIIEIAKESSTAGKKISCK"
BASE COUNT         2306 a         1539 c         1722 g         2512 t
ORIGIN      
        1 atcgtctgtg gcatccctgt actcgactcc cagatcataa tatttgatat cgatgtcaag
       61 gtagggaagg ataagcttgt ccttaataaa cttccatata atgcgtgtca tctcatcgcc
      121 atccagttcc acaaccggat tggccacttt aattttttcc aatactaatg atttttatgt
      181 tgaacaaata ttgatttcag agcacaaaaa tataaaaaca atgcgaaagg gtaggagaat
      241 caaggataat tttaatattc caatagcttt tgagaattat attcagaaaa gctgcacgac
      301 atgtaagaaa gtaataaaca aataaaggat gattaatttt aatttaatat ctttagccat
      361 gctataagac ctgaaatgag gtccaaatta tttcagatag aaaaattgtg ccgaagaagg
      421 ttgataggaa atattcctga catagaacgg cattctcgat acgctgatgg ggactacatt
      481 gattgaattg gaaaccaggc tttcatccag attgatgtac aactcgatgt catcttccgt
      541 caagatgtca tatttgctga ttccgactac acattttacg tccactgtat taggtatgga
      601 gatcacctta ttatccgcat tcttgatttt gattggtagg gtaaagctct tttcggtgaa
      661 ttcttccact tcaagattca tccttaccat gtcatggtcc agtcggatca ggtcggtctt
      721 tggagcttcc aattggatgg gcatatcgta aatatttcgg tttaatttat tgatatttaa
      781 gatttcagta ttccatttgg tgatgttctt tatggcttgg taagaacctg tgataaccac
      841 tgaatccgga gtgaaatgta ttggactttt gatataaaaa ctgggaagaa attcaatatt
      901 caattttgaa acaacaggta ctttcttttt agcttgatct accagtgaga tggaaatatt
      961 ggagggcttg atttgagcta ctgatatctc atcggaggca agtaatgaat tgattttctc
     1021 tgaaatggca gtagccggaa taagctggct gttatcggca ctcaactcaa cttcaatctt
     1081 atcaccgata tttttagctg atttgttgac aatatcccaa cctattccgg ttaatgtaac
     1141 ttcaaccgaa gtgacgggtg gcttcttgaa taccttgccg tcaggaatga tgtaactgac
     1201 ggaatacact tgtgtaaaac tataattgtt ggacaatttt tgcattgccc agaaaacaat
     1261 ggctacaatc acacaaatga gaaaaagcca cttatcccgg ttgacctttt ctgccattgt
     1321 ttatcaatta ttgattgaat cttgaattga acttcccata gcctcggtaa gctcttttga
     1381 gatggaacct ttcgttacct ttatatatgt ttttggatca acctgtaggg ttacgatgtt
     1441 tccctctatc ttgctgattc gaccgatgat tccacttgat gtgacgacat cattgccttt
     1501 cgcaagtgct tctccgaatt tttgctgctc tttttgtttt ttaatttgag gcctcaacat
     1561 gaaaaaatac attactgcga ccattccaag taataataat ggagaatata gaccttccat
     1621 aagtaattga ttatttttgt tttatttaag aatattggtt tttatcgtca gaagatattt
     1681 gctgggatat ccattggtaa atacggttac gacctttgac tgatagccgg atttgttttc
     1741 actgttaaat ctcaccagaa tttcgccctt ttcacccggt gcaatcggtt gtttggggat
     1801 ttccggaacg gtacatccac aactggaagt tgcatctttt ataagaagag gcacttttcc
     1861 ggcgttgaca aaatgaaatg tatgtgtcac gatgtctcct gccttgatat ctccaaaatc
     1921 atggctgacc tcatcaaatt tggctactgc tatattcacc gtgtccatat tctcatcggc
     1981 agtaatcgga ttgcgtatca attcctgata gtcattacca tcaccgagaa tttgctctac
     2041 cttcttttca tcggattcaa catcccgctt gcaacctact gttagtatgc ctaagcccaa
     2101 aataaggatc caaatcagct tcatcataac ttgaatgttg ttgtttaaaa tatatgattt
     2161 ttttatttat tacaacgacc caaaggtagt aaaatttaaa tgttggaact tattttttga
     2221 ttttacaaat atacttatct aataatcagt tgattatcgg gaggaatgag agtttgaata
     2281 aaatagtcct ggttatagga tgtcatgacc atactttcca agacagaata aaagatcaag
     2341 gcagcttaaa tttggcacga agcctgttct atcttcaaat acctgcaagt atcgcggact
     2401 ttcgaagagg gtataatttt ttaaaaggat ttgattcctt ttatcttccc ccaaatattc
     2461 atctacatac gagtcggaat atataatggt tacctccgga attaggtagg ttgcaatcat
     2521 tttcagaata ttccgattca gatcgagaag atacaccggt ctttctttta aaagtgcggt
     2581 gatttcgtca aagaaatata taaaataagc ggacttgcca tagcaggttt gtaaggagga
     2641 tagatgttga cttacccaat cttcgtcata gctgatcctg acatctgaaa atggttgcag
     2701 gttgtttttc cctttctgaa gtggtactga aagtaacaag ggcccttgtg aagcattgat
     2761 gtaacatttg tttctgaagc ttcctttcct gaaaaaatca cccgtgtcaa ttattacctt
     2821 tccccggcgc ttgaaggcag caaggacgga aatgggtgga aagtactggg actcgatgat
     2881 catcttcctt ttttggtctc aaagataata agcgctttaa attatatttt tgaggctaac
     2941 ctatgtttca taaaatatac ctttacatct tataattggt caaatgaata ttgaggtgtt
     3001 aaatactttc tccgacgctg aagctttttt atatagtagt ctcccgatgt tccaacggca
     3061 gggccctgcc gcttacaagg ccgatatcgg caatatttcc agattgatgg agactgtggg
     3121 taatccgcat tcctgtggca tcagaatgat ccatatagcc gggactaatg gcaagggtag
     3181 tgttacccac ctgactgctg cctacctgca agcgtgcggt tatagggtgg gtgtctatac
     3241 ttcacctcat tataccgata tccgggaaag aattaaaata aatggtacgc tgattggtga
     3301 agatgatttt gtcatatgcc ttcaagcatt tttaccattc attgaagaaa taaaaccctc
     3361 cttttttgaa atcatctgtg cgatggcctt cctgtatttc cgggatcaac aggtggattt
     3421 tgctgtaatt gaaaccggtc tgggtggcag actggatagt accaatatta tttttccaat
     3481 attgagtgtt attacgaata ttagtcccga tcatcaatcc atgcttggag acacgcttga
     3541 gaagatcgct tatgaaaagg caggaatcat taagaaaaat actccggtgg ttattggaca
     3601 aaaaatatcg gagacaattc aagtctttca agacaaagct gcatcagaag acgcttcgat
     3661 ttcttttgcg gaggataatt ggcagtgtat atataaatac aacgatagcc attataccca
     3721 ccttcaaatc agggatagaa tttccaacac ttccgccacg cataaaactg ccttgatggg
     3781 catttaccag actcagaata ttataacatt tctcgagata tgccgccaac tcagtcttta
     3841 taataatatt ccctttaatt gggagtatat atcaatagct ttaaaggaca ttgtgaagct
     3901 gacttattat atcggaaggt ggcagattgt tagccaaaag ccgctcatca tcctcgaaag
     3961 cgcccataat gttgcaggaa ttgaagcagt ccggaaacaa ttgagtggac tggaggtggg
     4021 tcaggtaacc atcgtctttg gaactgtttc ggataaggat gtagtacctg tactcaaatt
     4081 gttgcctaaa aaggcccggt atatctggac aaaagcggat atccctcgag ctatggacga
     4141 agtcgaattg tcggagaaag ctgcatttat cggtcttgca ggtatgacta gtcccaccgt
     4201 taaagatgcc ctacaacttg ccttagacca tacatcggag gatgatttga ttcttgttac
     4261 cggctccata tttgttgtgg gcgatgccat gacagggttg aaagaaaagg gaattttgtg
     4321 attctgacag agaaatagct gaaaaggtta tcttccggtc aacaatcgat ggattgattg
     4381 ccatttcatt tttattaaaa cctgaggcaa ttccgaatag tggatgataa gagaatcatg
     4441 aaagtttttg attatcaatc atagcttgtt taagcctgat tgtgaatcta tgttgccggt
     4501 accatgtatt ttaaggggat acgcacccat caaagaaaat aatctcgatg tggacatgtt
     4561 gccggtacca tgtatttaaa ggggatacgc accataggaa gtgccaacat atgttttctg
     4621 agggatatat tcacaacgtg ggaagtccgg aggaaaatgt ggtagcctga aggattttac
     4681 tacaattttg taatagatat gttttcggct tataaaggct gctattattg gccatcgcaa
     4741 ataaaatagt cattatcgat tatatatttg gcatgatata atattataaa tatttataat
     4801 gtgttggaaa tacgttctat atttttctaa acatgactta cctttgcctc atatttggaa
     4861 taagtaaatg aaagttaccg attatatcca tgaagccggg ggtaagaccc tgatttcctt
     4921 cgagattctg ccgcctttga aagggggaag tattcagaca attttcaagg ctatggaccc
     4981 tttgatggag tttaagcccc cctttattga tgttacctat catcgggaag agttcttata
     5041 ccagatgcag cctacaggtt attaccagaa aacatccata cgtaaacggc ccggtacggt
     5101 tggtatttgt gcggcgataa tgcatcgtta tggagtggag gcagtacccc acttaatctg
     5161 tggaggcttt aacagggaag ataccgagaa cgcactgatc gatctgcatt ttctgggtat
     5221 ccagaatgta ttggccctcc gtggcgatgc ccggcgattt gaagataagt tctctcctga
     5281 gcctgatgga catcattatg cagtagatct gattcaccaa attactggta tgaacaatgg
     5341 aagttatctt gataccaata tcgaaaaagg aagcagaaca gatttttgta taggcgtagc
     5401 cggatatcct gagaagcact ttgaatcccc gaacctcgaa ttggatataa aacatttaaa
     5461 agctaagatc gatgccggag gtaattatat tgtcacacaa atgttttttg acaataaggt
     5521 gtacttcgat tttgtgaaat tgtgtcgtga aaatggaata actgttccca tcattccggg
     5581 tctgaaacct ttgactaagc tttatcaact gaattctatc ccaaggaact tttatgtcaa
     5641 tctgcccaac gatcttgtca gggaagctca aaaagcgaaa acggatgagg acttgaagca
     5701 aattggcatc gaatggtgta ttgctcagtc cagggagttg gttgagaata atgtaccatg
     5761 tcttcatttt tacacaatga gtgatgctca ggtagtcaaa aagatttgtt ctaaaatagt
     5821 ataaggaata tgaaaagttg gttggaagtc atatcagatt ctgatttcag tatttataac
     5881 atcccttttg gcatcgctga gatcggcgga gagaagctga ttgtaaccag gctaggtgat
     5941 aaggtcattg tacttgatat gcttcaggat gccggtgtgt ttgctgagct ggatctgccc
     6001 tatgggatct ttgtagctga ttttctcaat gatatgatcg gcttaggcaa gccaatcaca
     6061 acatctatca gatcagcgat tcaaaaatat tttacggatg aggcctatgc acagcaattc
     6121 ggacttgatg ctggtcaata tctgattgat taccaattgg taaagatggc tatgccaatt
     6181 agagtgggtg actataccga cttttattct agtcgggaac atgctaccaa tgtcggcatt
     6241 atgttcaggg ggcctgacaa tgccctgatg cccaattggc tccatcttcc tgtcggctat
     6301 catggtcggt catcatccat tgttgtttcg ggaactgatt ttaagcgccc agccggccag
     6361 atgctggaca aggataccgg aaaaccgttt tatggaaaga gtcatgaatt ggatatcgag
     6421 gttgaaatgg cctttattgt aggtagaaat acgcatcttg gtgaaacaat cgatgtgaat
     6481 gaagctgagg attttatatt cgggatggtt ttattcaatg attggtcagc gcgtgacatc
     6541 cagcgttggg agtatgtgcc tttgggtcct ttcctgggga agaactttgc gtcgaccatt
     6601 tcaccctggg tggttacgat ggatgctctt gaaccattca ggactgcggg acctgtccaa
     6661 gatccggaag tattaaacta tcttgctacc cgtggatcaa aaagttttga cattccaata
     6721 agtgcgactc tgaccacaaa ggagggggtc gaaactgtga tctgcgagac aaattataaa
     6781 tttctttact ggaatcccag tcagcaattg gcgcatcata ccgtcaatgg ctgcaatgtt
     6841 aatatcggtg atttgatggc atccggtacg atttcaggct ctgatccctc gtcttatggt
     6901 tctatgctcg aactggcctg gaagggtacc aaaccaatcc agttggcaga cggtagtgaa
     6961 agaaccttcc tgatcgatgg tgattccatt actttaaggg caaaagcagt cagcggtgaa
     7021 gttgttgttg gtttcggaga agcgaaagga acggtattgc cataaaactg gcctcccaat
     7081 tctttcaggc aattgttgaa atttatttac agcttatctt ttttccggct gttgaagatt
     7141 cttttgctat ctcgatgatt ctgattactt cggcagcttg ttctggtgag accggattag
     7201 gaccccttcc attgaggaat tggtgtaatg catcaaaaaa ccaaaagtaa ttaccagggt
     7261 cggaaaatga ttctttccgt tttatattat tctgttcgtg ataggtcagg atgctatccg
     7321 gttctgacaa aggcttttgc cagttttcgg atgaaggaca tattccgact cccaattctt
     7381 tttcctgcat atctgaccga agttggctga gggaaccaag ctccccattt aatacatatc
     7441 cgggttccgg aagcgttacc atcacattgc tgataagccg gacccgcaat tgttggtaat
     7501 acagtaataa ttcaaaataa tcatcggatt cgacatccgg tctcaattgc atcagatcgg
     7561 cataaatatt ttctggaggt ccaaacaatt gtaaagcctg atcgatcaaa tggctcccca
     7621 gatcatacaa agcgccggat ccggggaagt tgccttcttt gtgtaatttt ccacttgatc
     7681 ctgtccggta acggtcaaat cttatcgttg cttcgtgaat ctgaccaaga tttccgcttt
     7741 caagaatagt cttcaccctc aggtagtccc ggtcgaatcg gcgattttgg aaaaccgaaa
     7801 gctttgcgtt atgttctctg gcaatatcaa tcaattccag cgcctgacga taggtaaccg
     7861 taaagggttt ctcaaccaaa acatgctttc cggttgcaag tgcttgtttt gcaatttcat
     7921 aatgagtctg aacaggagtg ttgactacaa tcaagtcaat gtccggcttg gcaatcaaag
     7981 cttcagtgga ccggagtatc tgcgtatcgg gatattccgt gattgatgcg ggattagatc
     8041 ttcttacgat gtaacccaaa gaaaatcccg gatgaacgg
//
LOCUS       DAOQMC010000101         7929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3679_length_7929_cov_4.154305, whole genome shotgun sequence.
ACCESSION   DAOQMC010000101 DAOQMC010000000
VERSION     DAOQMC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3679_length_7929_cov_4.154305"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..155)
                     /locus_tag="PKV31_05365"
     tRNA            complement(84..155)
                     /locus_tag="PKV31_05365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(121..123),aa:Thr,seq:ggt)
     gene            complement(223..295)
                     /locus_tag="PKV31_05370"
     tRNA            complement(223..295)
                     /locus_tag="PKV31_05370"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Gly,seq:tcc)
     gene            complement(346..431)
                     /locus_tag="PKV31_05375"
     tRNA            complement(346..431)
                     /locus_tag="PKV31_05375"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Tyr,seq:gta)
     gene            complement(445..518)
                     /locus_tag="PKV31_05380"
     tRNA            complement(445..518)
                     /locus_tag="PKV31_05380"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Thr,seq:tgt)
     gene            complement(851..1165)
                     /locus_tag="PKV31_05385"
     CDS             complement(851..1165)
                     /locus_tag="PKV31_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HNB92320.1"
                     /translation="MEGSRNKELDWLRIIAWGEGTSFLLILFVSMPLKYIWEMPMANK
                     IIGYVHGVLFILYIITVWLIAEKREWSTRVRVISLVASLIPFGAFWADRRIFARKVDS
                     GK"
     gene            complement(1261..3006)
                     /locus_tag="PKV31_05390"
     CDS             complement(1261..3006)
                     /locus_tag="PKV31_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNB92321.1"
                     /translation="MKRSFFLAVLCLIGWRGSSQETFPIYNFKQEVEENRSVTYDKAI
                     ELWKIMSLYDKRLVVRETGKTDVGRPLHEVIVDGDSDFDPVKARRRTKNVIFIINGIH
                     PGEPDGIDATLWLVQDLLTDPKKGELLKNVVLVIIPVYNVGGALERNSTLRVSQNGPE
                     WYGFRGNGQYLDLNRDFIKCDSKNALSLVSIFTKWDPEILIDNHVSNGADYTYVMTML
                     VSQPDKLGHGQDAFLRQKMLPDLYRRMEKKGFEVPPYVNVWRSTPDKGLPGFMDTPRY
                     SSGYGSLFQTYCFVPESHMLKPYDQRVKATYAFMMSMLEYHAKNFIDIKISRSKAINE
                     VRDAARLPVLWSFDRSRADTFLFKGYDYEYIASEVSGLLRLKYRRDKPVTWKVPFWNY
                     ADAVVEVDVPKAYIVPQAYARVVERLDANRVKYLPIRQDTIIKGKMYRIVSTDSPKEP
                     YEGHFGHSNTRVEEFEAEKKFLRGDLIVYTDQRTRRYLVETLEPQGHDSFFNWNFFDG
                     ILDHKEGFSDYVFEDVAAEMLRNDPDLKKSLEQAKENDPKLRESAYGQLNYIYEHSKY
                     KEPWQNVYPVMRVMN"
     gene            3736..4815
                     /gene="pdxA"
                     /locus_tag="PKV31_05395"
     CDS             3736..4815
                     /gene="pdxA"
                     /locus_tag="PKV31_05395"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNB92322.1"
                     /translation="MTLPKIGFSIGDTNGIGLEVIIKSLLSNRFSNRFIPVIFGSPKI
                     INYYRKMLSIDDNIFQFLKVGENPRPKKVNIFTCWEEEATISHGEVSREGGKFAKLSL
                     EQAIESLKSKYIDSLVTGPIHKKSMELAGFGFPGHTEYLEKSFPPQQSLMLMVYEDLR
                     IGVVTGHIALHEVASGINPKAIITKAKIMLQSLKVDFGIEKPVIAVLGLNPHAGDKGM
                     FGNEEENIIIPAINDLKKDGHIVMGPYPADGFFGSGLFKKFDGILAMYHDQGLVPFKT
                     IAFGGGVNFTAGLPVVRTSPDHGTAFDIAGKNIANPSSLRHATYLALDILRNRNEYSN
                     LVANRLISRKDKYEGEDEIIEDLEG"
     gene            complement(5013..6653)
                     /locus_tag="PKV31_05400"
     CDS             complement(5013..6653)
                     /locus_tag="PKV31_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB92323.1"
                     /translation="MFLNICIILQSLFFPWLNNEHYQTPTLEKLNTRINTDAYDETNP
                     VVSKDGLTIYFTRVGHPDFNRTLMDDNTDLSATLSFADYQQELSSIYQTLSGRPVRDI
                     SQNSFNQDIWIATWAGGDFNNIEHPDYPLNSALPNSICSLTPDPDKFIIVNEFYKDGS
                     MYRGFSTIQDLGNGNWSFPKPLFIYDYFTQGNEVNLTMDEDGRIIIMSLKRPSGKGDN
                     DLYVSMKVADNLYSTPLPLNGVINTAYRESTPFISRDGQFLYFSSNRPGGYAGNDIYV
                     SRRLDETWQNWSEPALLPKPINSESDDSQPFVNEASGYIYFTSRRDGTSDIFRTSFHP
                     VKEEERKKLLIVHVVNALTGQPLDAKIALGKQKDGFFTRTIEAKTGKANYSFVEEDNL
                     IMKPKKSGYKAEKVEVNLPEMYKDDATISEITLYMDPEGDSERFTLDNIYFVQSMAII
                     KDESYPVLDRIIPIFKRRPDLKITIYGHTDNIGRENDLVELSQQRADAIKNYLVQKGG
                     IAADRIKTVGKGRSMPVNDNSTEEKRAKNRRVEFAVYK"
     gene            6823..6978
                     /locus_tag="PKV31_05405"
     CDS             6823..6978
                     /locus_tag="PKV31_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92324.1"
                     /translation="MNIVKQQMLMFELAPKGNPEILFDMSDKKDLNVGFKLTKCKRFE
                     VANQVAE"
     gene            7024..>7929
                     /gene="lpxK"
                     /locus_tag="PKV31_05410"
     CDS             7024..>7929
                     /gene="lpxK"
                     /locus_tag="PKV31_05410"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNB92325.1"
                     /translation="MAFKVKRKNPLLSITFLLLPFAILFGIIVYLRNLLYDLKVIRPV
                     RFSVPTIGVGNLAVGGTGKSPHIEYLVKTLTPYIHVASLSRGYGRKTTGTIEVMNNAT
                     SEQVGDEPLVLKMKFPDISVFVGSNRAFAIPELMGRKPQTQVVLLDDIFQHRSVIPHE
                     NILLTEYSLPYFGDFLLPVGALREMPMGAKRADTIIVTKCPPEITLEQEEYFRKKIKP
                     GPNQQVYFSTFSYGDPYRLFQPTQRIKLQESMEIILFSAIAGTDYLMEYLKTQTKMIH
                     SIEFQDHHFFTGDEILQLKNTYTVLN"
BASE COUNT         2316 a         1781 c         1473 g         2359 t
ORIGIN      
        1 actgtttact gatagtgaaa atggccgttg ccacttttgt gtcaattatc aattatcact
       61 aatcaataat agttcatttt tagagccgtt gatgagaatc gaactcgcga cctcttcctt
      121 accaaggaag tgctctacca ctgagctaca acggcattgc tcagttattg gcctcggcca
      181 acgatatgca attacgttaa ccgtttacct ataaccaaca tgagcgggag acgaggctcg
      241 aacccgcgac ctacagcttg gaaggctgtc gctctaccaa ctgagctact cccgcaaatg
      301 gttgatttat cttgagtcat tgaaagggac accctgcaaa aaccttggtg ggtagagaag
      361 gattcgaacc tccgtactcg tgagagaaca gatttacagt ctgtcgcctt taaccactcg
      421 gccatctacc ctacacataa acagagccag tggagggatt cgaacccccg accagctgat
      481 tacaaatcag ctgctctggc caactgagct acactggcat tgaatttcgt tctactttta
      541 tcggagcttt ccggccaaag tacaaggatt tcaacttact ttattttcaa gcgcaatatt
      601 agcgccaatt cggtcttttc aatcacttcc tatgaacttt ttcataagcg actgcaaaga
      661 tacgactaac tttgatattt caaatatcct cttgaaaaaa aatcaatttt tttttatttt
      721 ttttggccag acaactttat taatacacaa catcatgatt atcaacataa taccatttca
      781 tgaattatcg attgcctgaa attcctgtcc aacatttgta ataggattgc agaacaaatc
      841 catacatcca tcatttgccg gaatcgactt tccgggcaaa tatacgccta tccgcccaaa
      901 atgccccaaa aggaatcaag gatgcaacca aagatatgac tctaaccctg gttgaccatt
      961 cacgtttttc agcaatcagc cataccgtga ttatatataa gataaagagt acaccgtgta
     1021 catagcctat gatcttattg gccattggca tttcccaaat atatttcaac ggcatggata
     1081 caaagagtat aagcagaaat gatgtacctt ccccccaagc tatgatcctc aaccagtcta
     1141 attctttatt tctacttcct tccattaaat cttagtttca ctgccctccc ttaacaaaga
     1201 ggatggcatg aagttcaata tttatgtctt tttttgagct tttcataaac aaatacaagt
     1261 ctaattcatg actctcatca ccggatagac attctgccag ggctccttat atttggaatg
     1321 ctcgtaaata taattcaatt gtccataagc gctttctctc aattttggat cattctcctt
     1381 agcctgctcc aaggattttt tcaaatcagg atcattccgc aacatttcag ccgctacatc
     1441 ttcaaataca taatccgaaa atccttcttt atggtctaat ataccatcaa aaaaattcca
     1501 gttgaagaat gagtcatgcc cctgaggctc caacgtctca accaaatacc ttctggtacg
     1561 ctgatccgta tatacgatca ggtctcctct caaaaacttc ttttctgcct caaattcttc
     1621 tacccttgtg ttcgagtggc caaaatgccc ttcatacggc tcttttgggc tgtccgtgct
     1681 tacaattcgg tacattttgc cctttatgat tgtatcctgc cttatgggca ggtatttaac
     1741 ccgattagca tcgagccttt caacgactct tgcatacgcc tgtgggacaa tatacgcttt
     1801 cggcacatca acctccacaa cagcatcggc atagttccag aatgggacct tccaggtaac
     1861 gggtttatcc ctgcgatact tcaatcgcaa caaccctgaa acttcactgg cgatgtattc
     1921 ataatcatat cccttgaaca ggaaggtgtc tgccctgctt ctgtcaaaac tccacaacac
     1981 cggcagcctg gcagcatcac gcacctcatt gattgctttg gaacggctta tcttgatgtc
     2041 aatgaaattt ttagcatgat actccaacat ggacatcata aaagcataag tagccttgac
     2101 ccgctggtca taaggcttca acatgtgcga ctccggaaca aaacaataag tctgaaataa
     2161 agatccatag cccgacgaat atctgggtgt atccatgaat cccgggagac ccttatcggg
     2221 tgttgatcgc cacacattga cataaggtgg aacttcaaat ccttttttct ccatacgcct
     2281 atacaggtca ggtaacattt tttgacgaag gaacgcatcc tgtccatgcc ccagtttatc
     2341 gggctggcta accagcatgg tcatcacgta tgtatagtca gctccattgg aaacatggtt
     2401 atcaatcagg atctcagggt cccacttggt aaaaatggac accaggctta gcgcattctt
     2461 cgaatcacat ttgataaagt ctcgattcag atccagatat tgtccattgc ctctgaagcc
     2521 ataccattca ggtccgtttt ggctgacacg aagagtcgag tttcgctcca gagcaccacc
     2581 gacattatac accggaatga tcaccaatac aacattttta agcaattcac ccttcttagg
     2641 gtcggtcaaa agatcctgaa ccagccacaa agtagcatca attccatccg gctctccggg
     2701 atgaatacca ttaataataa aaatgacatt cttggtcctg cgtcgagcct tgacaggatc
     2761 aaagtcgcta tcaccatcga cgatgacctc atgcaggggc cggcctacat ctgtcttgcc
     2821 tgtttctctt accaccaacc gcttatcata taaagacatt atcttccaca attcaatagc
     2881 tttatcataa gttaccgacc tgttttcttc tacttcctgc ttaaaattat aaatcgggaa
     2941 agtttcctgg ctgctaccac gccatccaat caagcaaagt actgctaaaa aaaaacttct
     3001 tttcattttt agagaattta aggctgcaat ttatccaaaa atcatttact tcaaaagtat
     3061 tatctgtgca gcactgttaa acgacgttcc atcattgagt cataattatt caatatactg
     3121 acaagcatac ttagcgactc ctccaatagt gaccttgtcc gatacagttg caatcattgg
     3181 taaattgaat ttctctaaaa gcaaatattt cacaggaatc atagaaattg tgccgctaaa
     3241 aaacgctacc cgggaatttt tcttgttttt attataacta catatcaaat tctctatgga
     3301 atactgttcc ccaatccatt ctcccccttc gaacagtgaa taaattaatc attccggatt
     3361 accccatcca ttttcggcaa caagctcatg tgtttctttg atcatagata tagattgcat
     3421 gctccgcttg agctaaattc catctttgag cagatcaaac gaatgcaggc ccacctgaat
     3481 acatcaattt tttatatctc gaataatttt gctcttttgg gtccgtgccc tgttcaacag
     3541 ctcaaatcag aatagggctg gtcatcaatg aagtttgatt gagttagccc ttgtttacat
     3601 ttattacatc tactttgacc tgccttccca accccattat tttcaattga ttattgattg
     3661 aatttggaaa tgttacggat acatcttaac ctatacggag gaaacttcta tatttgcgac
     3721 acaaaatttt tccggatgac acttcctaaa atcggatttt ctataggtga tacaaatggc
     3781 atcggccttg aggttattat caaatccctc ctcagcaacc gtttttcaaa ccgttttatt
     3841 cccgttattt tcggctcgcc caagataatc aattattatc gcaagatgct atccattgat
     3901 gataacatat ttcagtttct taaggttgga gaaaatccaa ggccaaagaa agtaaatata
     3961 tttacttgtt gggaagaaga ggcgactatc agccatggag aggttagccg ggaaggtgga
     4021 aaatttgcca aactttcact tgagcaggcc attgaaagtc ttaaaagtaa atatatagat
     4081 tcactggtta ccggtcccat ccataaaaaa tctatggagt tggcaggatt cggttttccg
     4141 ggacatacag aatatctgga aaagtccttc cctccccagc aatcactgat gctgatggta
     4201 tacgaggacc ttcgtatcgg tgttgttacc ggacacatag ccttacacga agttgcttcc
     4261 gggatcaatc cgaaagcaat tatcaccaag gcaaagatta tgcttcaatc cctaaaagtt
     4321 gacttcggaa tcgaaaagcc tgtaatcgct gtattgggtt taaatcctca tgccggagac
     4381 aagggaatgt ttggcaatga agaggaaaac attatcattc ctgctattaa cgatttaaaa
     4441 aaggatggtc atatcgtgat gggaccatat ccggctgatg ggtttttcgg ttcgggactt
     4501 ttcaaaaaat ttgatggtat acttgccatg tatcatgacc aggggctggt tccattcaag
     4561 accattgcat ttggcggagg agtcaatttt actgcaggcc tgccggtcgt aaggacgtct
     4621 cctgatcatg ggaccgcttt tgatattgcc ggtaaaaaca tcgccaaccc ttcatccctc
     4681 cgtcatgcaa cttatcttgc gctggacata cttcgcaaca ggaatgaata tagcaatttg
     4741 gtggctaatc gccttatctc cagaaaagac aaatatgaag gtgaagacga aatcatcgaa
     4801 gatctggaag gataaaaaag tccaaccgat ggtgtcagaa tggttttgtg catgatattc
     4861 tgtttttggg actatcggtt ggacagtaaa agactaaagg tttacattct gcatatatac
     4921 atgcaaaaca tatgccaaaa atttttaatt taagattttt aaggaaaatt tagtaaaaat
     4981 gaatttatat attgataata taaatatata aattacttat aaacagcaaa ttctaccctt
     5041 ctgttttttg ctcttttttc ttcggtagaa ttatcattta caggcatact tctacctttt
     5101 ccgaccgttt taatccgatc agcggctata cctccctttt gtaccagata attcttgatg
     5161 gcatctgccc tttgctgaga taattcaacc aggtcatttt cccgacctat attgtctgta
     5221 tggccataaa tggtgatctt caagtcaggc cttcttttaa atatcgggat gattctgtca
     5281 agtacagggt aggattcgtc cttgattatt gccatagatt ggacaaaata aatattgtcc
     5341 aaagtaaagc gctcactatc cccttccgga tccatataaa gagttatttc cgatatagtt
     5401 gcatcatctt tatacatttc cggaagattg acttcaacct tttccgcctt gtaccctgat
     5461 ttcttgggct tcataataag gttatcttct tcaacgaaag agtaattggc cttgccggtt
     5521 ttagcttcga tagtcctggt gaagaatcca tccttttgct ttcctaaggc aatctttgca
     5581 tcaaggggct gtcctgtcag ggcatttacc acatgaacaa tgagaagttt ctttctctct
     5641 tcttccttga cgggatggaa tgatgtcctg aaaatatcac ttgtcccatc tcttctggat
     5701 gtaaaataaa tatatccgct ggcttcattg acaaagggct gagaatcatc ggactctgag
     5761 ttgatgggtt tgggcaataa agccggttca ctccaattct gccatgtctc atccaatctc
     5821 ctggagacat agatatcatt gcctgcatac cctccgggtc tgttggacga aaaataaaga
     5881 aactgcccgt cacgtgatat aaatggcgtg gattctcgat atgccgtgtt tatcacacca
     5941 tttaaaggaa gaggggtaga atacaggttg tctgccacct tcatacttac atacaaatca
     6001 ttatcccctt tgccgctcgg tcttttcaat gacatgataa tgatacgtcc gtcttcgtcc
     6061 atggtgagat tcacctcatt gccctgagta aaataatcat agataaacaa tggtttggga
     6121 aaagaccaat tgccattgcc caaatcctgt attgttgaaa acccccgata catactccca
     6181 tccttataga attcattgac tataatgaat ttgtccggat ctggtgttag cgaacatata
     6241 ctattgggta atgcactatt aagaggataa tccggatgct cgatgttatt aaagtctccg
     6301 cctgcccagg tggcaatcca gatatcttga ttgaagctgt tctgtgaaat gtccctgacc
     6361 ggtctcccgg acaaggtttg atatatggat gatagttcct gttgatagtc ggcaaaagac
     6421 aatgtcgcac tcaggtcggt attatcgtcc atcaaagtgc ggttgaaatc cggatgtccc
     6481 actcttgtaa aataaatcgt aagcccgtcc ttgctgacta ccggattggt ttcatcataa
     6541 gcatccgtat taatccgggt atttaacttc tcaagggtcg gcgtctgata atgttcatta
     6601 ttcaaccaag ggaagaaaag actttgcaat atgatacaaa tattgagaaa cattacagat
     6661 tgatcagtta attaaataca tcaaagtttg tcacccgggt aaattcctcc gcaaagatat
     6721 aaaaatttat aatatgactg gagaagttac cggaaaagat tcgcttaaga gtgaataaat
     6781 aagaggcgtg ttagttcgtt ccgtgcaaat ttacaatatt aaatgaacat tgtaaaacaa
     6841 caaatgttaa tgtttgaatt agctcccaaa ggcaatccgg aaattttatt cgatatgagt
     6901 gataaaaagg atttgaatgt gggttttaaa ttaacaaaat gcaaaaggtt tgaagttgca
     6961 aatcaagtag ccgaataacc gaattaactc ttccgtccca tatgcttaaa atagactttc
     7021 cctgtggcct ttaaagtgaa acgcaaaaat ccgctgctat ccattacatt tctgttgtta
     7081 ccctttgcca tacttttcgg catcattgtc tatttgagga atttactgta tgacctgaaa
     7141 gtgatccggc ctgtcagatt ctctgtaccg acgatcggag tcggcaatct ggctgtggga
     7201 gggaccggta aatcaccgca tatcgaatat ctggtaaaaa cactgacacc atacatccat
     7261 gttgcttcgc ttagtcgggg atatggacga aagacgaccg gaactatcga ggttatgaat
     7321 aatgctactt ccgaacaggt gggtgatgag cctttggtgt taaaaatgaa gtttcccgat
     7381 atcagtgtat ttgtaggctc caaccgggca tttgccattc cggaactcat ggggcggaaa
     7441 ccacagacgc aggttgtcct tctggatgat atcttccagc atcgttcagt gatacctcat
     7501 gagaatatat tgcttacaga atacagcctc ccctactttg gcgacttcct tctaccggtc
     7561 ggtgcactca gggaaatgcc catgggtgca aagcgggccg atacaatcat cgtgaccaaa
     7621 tgccctcctg aaatcacttt ggaacaagaa gaatatttcc gaaaaaaaat caagcccggg
     7681 ccaaatcagc aggtatattt cagcacattt tcgtatggag acccctaccg acttttccag
     7741 cctacacaaa ggatcaagct tcaggaaagc atggaaataa tcttattttc agcaatcgca
     7801 gggacggatt atctgatgga atacctaaaa actcagacca agatgatcca ctccatcgaa
     7861 tttcaggatc atcacttttt tacaggagat gagatcctac agctaaaaaa tacctataca
     7921 gttctgaat
//
LOCUS       DAOQMC010000102         7913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3690_length_7913_cov_4.815984, whole genome shotgun sequence.
ACCESSION   DAOQMC010000102 DAOQMC010000000
VERSION     DAOQMC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3690_length_7913_cov_4.815984"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..2025)
                     /locus_tag="PKV31_05415"
     CDS             complement(121..2025)
                     /locus_tag="PKV31_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNB92326.1"
                     /translation="MVSAQEILKKYWNYNSFKPVQEDIIRHITEKGDAFVLMPTGGGK
                     SLCFQIPALMMKGMTIVITPLISLMKDQVMRLKSLGINAEAIYSGMTKMDIHRILDSC
                     LTGKIKLLYLSPERINTSLFKEFANGLHVDLIVADEAHCISQWGYDFRPAYLKIREAR
                     KIFPKARIMALTASATPEVVNDIMEHLSIGDAAIFKLSFLRSNLSYVCRNTTDKRFEL
                     LNLVRKVPGTGIVYCRNRRATAEIASFLSGHGISADFYHAGLDQKRLSDVQRGWMEGK
                     IRVIVATTAFGMGIDKPDVRFVAHLALPPSPEEYYQEAGRGGRDGKHAYAALFYDDED
                     IHELRESIDSNFPGKEQLVKLYHRLALYFHVGAGPLEEQYFDFDINLFCKEYGYTPVW
                     VVKGLKELERQGHVYVTEGIYLPNQVMILYSKSELDTFRSRQPDLIPILEVLLRLYGG
                     IMSIPVKINIKAIGQKLRTTSENVLAYLNILANEGVIWFQPQKDKPQISFGEFRYVSD
                     SLRFDFSLQEFLKKNYKARVEAMISIVEQEVCRNKTLLSYFGEAYTHNCGICDVCLQE
                     KRIENKDSGLSRTIEYVMNFATSEHNDLQTLLSTTYYRANKEKVDAALQFLVQEGTIV
                     VDNFKIVKAE"
     gene            complement(2071..2928)
                     /locus_tag="PKV31_05420"
     CDS             complement(2071..2928)
                     /locus_tag="PKV31_05420"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNB92327.1"
                     /translation="MNKNRILYLFLCFPFFASGQFSEVGIGLGGAVYYGDMSLDKAVD
                     NFKMVRPNIGIFANQHLNERIAVQLGIQNFTLTGDDALNSRQGPRERNLNFRSNIWEI
                     ALRGEFYLIPFDPDKSKLPFSLYMSAGVAVFFHNPKAYYAGSYYALRPLSTEGQGLPS
                     FPDRKPYSLTQIAFPVIGGLKYMVSKGVNVFIEFGPRFTLTDYIDDLSNSYAVENELR
                     YYKGDIAVALSDRRLVNGSEPKRYPDNQQRGNPKSNDMYFTGLLGVSFNMNDFFGLEL
                     INKVKCPTF"
     gene            3252..4907
                     /locus_tag="PKV31_05425"
     CDS             3252..4907
                     /locus_tag="PKV31_05425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNB92328.1"
                     /translation="MKKSFFLPFLAFLITLNCLNAQQVAPENWFTTDIMESGVPGTRV
                     DKAYKELLKGQQPKPVIVAVIDSGVEADHEDLKDRMWVNPKEKPGNNIDDDKNGYADD
                     INGWNFIGGKDGRNIKQDSYEATREYVRLKAKFDGADPAKISRKDQKDYNEYVKLKED
                     IDKKRESAANNLNQIEMTVTMLSAPLQSVKEALGRKKATKENIDAIDADDDQNLNMGK
                     MIMAQILGSGEFPTQNMDSIILLVESELNEGIKHYKNQIDYSYNTEFDPRTDIVRDNY
                     SDYTEKSYGNNDVEGPEASHGTHVAGIIGANRNNNLGIRGIADQALIMSLRAVPDGDE
                     RDKDIANSIIYAVDNGAKVINMSFGKGYSPGKKYVDAAVKYAAKKDVLLVHAAGNSSA
                     NNDTEPNFPNARFEKKGLFGKKFADNWIEVGALSWRPGEDMVAGFSNYGAKNVDLFAP
                     GVDLYSTVPNQSYKKESGTSMAAPVVAGVAVLLRSYFPTLSAKQTKEIILQSVHKVNA
                     MVKKPGTDELVPFSSLSVTGGYVDAYEAIKLAAKKTGVKLAMP"
     gene            complement(5012..7660)
                     /locus_tag="PKV31_05430"
     CDS             complement(5012..7660)
                     /locus_tag="PKV31_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB92329.1"
                     /translation="MNILYGNLQCEYLIPKKVESQVFPPERPTILPFSPCSEYTLKVL
                     LSADTLRDEAHWSITGTSDFRRIGDTLFVTWHQTGTFKVCVWATNECGTSDTLCQDVT
                     VGQKPVFDILTDSISCNGLFHIEVKSQFQNLTWVDIQGNSIFSVNGDGSLVAGSILVG
                     KNSAVLKYSGSIAGCDLFGSLKFYRQEPPKFIVKDTAFCGSGTYRIPVEIVNHAGELY
                     YSFEGQGFHMPVDIGLNYIDLDFNASGGVYIDSLKTQYRTCNDLQSDTLDVRIENMPE
                     SLLPDTITLCNTYNRDGKPVYFLPDLIVSGERSGNWNLSSLSGITISNDSIDMSHVAV
                     GTYKVNFRTNTAVLPCKDVTYATVFKVVDCSCNRPALNKLKDTYRICSNYGSLNLDSL
                     AGVNVPVQWFDLTSGSKEAIGGSNLLINDQFVGVHSILLQTVADWEGACADTILFELQ
                     VVPRFYAGDDYLMDICNGEDYLIDLDTLLINSLNDGAWKAQKPDYPGFSIAFDPQTNT
                     ISTKKLFPGDYMLDKIVAGSDVCPSDTASLTIKVYPLPEILVSGTNYLDCESQVGEIK
                     VDPGASSSIRTDWIFQNDIIHTSISLDSAIIDKAGTYYFSSQDQITGCRDTVSFLVGD
                     NSVPIDTVTYRLIGGCQENGSALILDYPIGGTPPYSYSLDNSSFTSYTQFYDLNAGLH
                     QLRVLDSKGCRFLLDFQVDDFVSGSLHLGRDTTIQLGDEIWVQVDFPQELIEWIGVSL
                     NGTPTVIKPEGEWLKPIETTTIQVLIRDKNDCLHEASRVIFVENDTKLFMPNIFSPNA
                     DGRNDVFFVPANRKVEMIMELHIYDRWGNQVFSLSNFVPGEEANGWDGTFHGRKMNPG
                     VFVYYVRYRTIDGKEALAKGDFTLLR"
BASE COUNT         2535 a         1739 c         1450 g         2189 t
ORIGIN      
        1 attttcgggc aaatactata atctcatacc ctgcttgttc caggcttctg atacttttct
       61 gcagtcgttg atcaaacagt atatctccga atgtaagtaa gattatttta ttgcctcttt
      121 tcactctgcc ttgacaattt taaagttgtc aactacaatc gttccttcct gaaccaagaa
      181 ctgtaacgcg gcatcaactt tttctttatt agcgcgataa taagttgtgg atagtaaggt
      241 ctgcagatcg ttatgttctg atgtggcgaa attcatgaca tactctattg tccgggaaag
      301 tccggaatct ttattttcaa tgcgcttttc ttgcagacaa acatcacaga tgccacaatt
      361 atgtgtatat gcttctccga aatatgataa caatgtcttg tttctacata cttcctgttc
      421 cacaatagaa atcatagcct ccacacgagc cttgtaattt tttttgagaa attcctgcag
      481 gctaaaatca aaacgcaggc tatccgaaac gtacctgaat tcgccaaagg aaatctgtgg
      541 cttatccttc tgaggctgga accatattac cccttcattg gccagtatat tcaaatatgc
      601 taacacattt tcagatgtag tcctcaactt ttgccctatg gcctttatat tgatcttgac
      661 aggaatactc ataattccac catatagcct caaaagcacc tcaagaatgg gaataagatc
      721 cggttgccgt gaccgaaaag tatccaactc agatttacta taaagaatca tgacctgatt
      781 aggtaaataa atgccctccg taacgtaaac atgtccttgc cgctccaatt ccttgagccc
      841 cttcacaacc cacaccggtg tataaccata ttctttacaa aataaattga tatcaaagtc
      901 gaaatattgc tcttccaggg ggcctgcccc tacatgaaaa tataatgcca acctgtgata
      961 caacttcact agttgctcct ttccggggaa attggagtct attgattccc ttaattcatg
     1021 gatatcctca tcatcataaa acaaagctgc atatgcatgc ttgccatcac gcccacctct
     1081 tccggcttcc tggtaatact cctcggggct gggaggcaat gcaagatgcg ctacaaatct
     1141 gacatccggc ttatcaatac ccataccaaa agcagtggtg gcaacaatca ctcttatctt
     1201 tccttccatc caacctctct gcacatcgga taatcgtttt tgatccagtc cggcatgata
     1261 gaaatctgct gaaattccat gacccgataa aaaggaagcg atttcggctg tagcacgtct
     1321 gttccgacaa taaactatcc cggtcccggg aactttccgc accagattaa gtaattcaaa
     1381 tcgcttatca gttgtattac ggcatacata ggacaggttg ctcctcaaaa agctcagttt
     1441 aaaaatagct gcatctccga tacttaggtg ctccattata tcattcacta cttccggagt
     1501 agcagaagct gtaagtgcca tgatacgtgc tttagggaaa atcttccgtg cttcacgaat
     1561 ttttaggtat gcaggccgaa aatcatatcc ccactgtgat atacaatgcg cctcgtcagc
     1621 aacaatcaaa tcaacatgca agccattggc aaactcctta aacaatgatg tgtttatccg
     1681 ctcaggagat agatataaca acttaatctt accggtcaaa caactatcga gaatcctgtg
     1741 gatatccatt tttgtcatac cactgtaaat agcctcagca ttaatcccta atgacttaag
     1801 tctcatcacc tggtccttca tcaacgaaat taaaggcgta atgacaatgg tcattccttt
     1861 catcatcaaa gccggaatct gaaaacacag agacttacct ccgcccgtag gcataagcac
     1921 aaatgcatct cctttttcag taatatgtct gatgatatct tcctgtacag gcttgaaact
     1981 gttgtaattc cagtatttct tcaatatttc ctgtgcagat accacgaaag aacttttatt
     2041 gaactaccaa actgattata actttacata ttaaaaggta ggacacttga ctttattgat
     2101 tagttcaagc ccaaaaaaat cattcatatt gaatgatact cccaagagcc cggtgaagta
     2161 catatcattc gattttggat tacctctttg ttggttgtca gggtatcttt tgggttctga
     2221 gccattcacc aatcttctat cagacaacgc gacagcaata tctcctttat aatatctcaa
     2281 ttcattctca acagcatagg aattgctcag atcatcgata taatcagtca gggtaaacct
     2341 aggcccaaat tcgataaaga cattaacacc ttttgatacc atatacttga gtccgccgat
     2401 gaccggaaaa gcaatctgag tcaaactgta aggctttcgg tcaggaaaag aaggtaggcc
     2461 ttgcccttca gtgctcaatg gccttaaagc ataataacta cctgcataat atgctttggg
     2521 gttatgaaag aaaacggcga ctccggctga catataaaga gaaaaaggca gttttgactt
     2581 atcgggatcg aatggtatta aataaaattc accccgtagg gctatctccc aaatatttga
     2641 cctaaagttg agatttcttt ctcttggacc ttgacgagaa ttgagtgcat catcgccggt
     2701 aagggtgaaa ttttgaattc ccaattgaac agcaatcctt tcattcaagt gttgattagc
     2761 aaagatacct atattgggcc tgaccatctt gaaattatct acggccttat caaggctcat
     2821 gtcgccataa taaactgctc cgcctaagcc gattcccacc tcactaaatt gtcctgaggc
     2881 aaaaaatgga aaacacaaaa aaagataaag gatcctattt ttattcaaag ctgtttctcg
     2941 tttagaatgt tgtaattcgt cttacaatga tgcacaaagg taagcattta taataaatta
     3001 ccttttttta tatatctggt ctatcaaatc cagctatttg cagaaaaagt ataaaagcaa
     3061 caaatcgatt ctataccttt aatccgttaa atagattgta aatgaagggt tcttaacaaa
     3121 attcttttct acataacgca aaatatagct accatgatac aattgtttta aaaatttctt
     3181 tcttttgtgg acaataattc attgcttgta catcaatacc attcatatta aattcaacta
     3241 aatatttata catgaaaaaa tcctttttct tgccgttttt ggcattttta attacattga
     3301 actgtctgaa tgcccaacag gtagctcctg aaaattggtt taccaccgat atcatggaaa
     3361 gcggagtgcc cggcaccaga gtggataaag cctacaaaga attgctaaaa ggccaacagc
     3421 ccaaaccagt aattgtagca gtaatcgata gcggtgtcga agctgaccac gaagacctca
     3481 aggatcgtat gtgggtaaat ccaaaggaaa aaccgggtaa taatatcgat gatgacaaga
     3541 acgggtatgc tgatgacatc aatggctgga actttattgg gggtaaggat gggcgcaaca
     3601 taaagcagga ttcttacgaa gcaactagag aatatgtcag gcttaaggct aaatttgacg
     3661 gtgcagaccc tgccaaaata agccgcaaag atcaaaaaga ttacaatgaa tatgtcaaac
     3721 tgaaagagga catcgataaa aaaagggaat ctgcggcgaa caacctgaat cagattgaaa
     3781 tgactgttac aatgctgagc gcaccactgc aatcagttaa agaagccctt ggaagaaaaa
     3841 aggctacaaa agagaatatc gacgccatag atgcagatga tgatcaaaat ctgaacatgg
     3901 gaaagatgat tatggcccaa atcctgggtt ccggagagtt tcctacgcag aatatggatt
     3961 cgataatcct tctggtagaa tctgagttga atgaagggat aaagcactac aaaaatcaaa
     4021 tcgattattc ttataataca gagttcgatc ctcgcacaga tatagtcagg gacaattaca
     4081 gtgactatac tgagaaatct tatggaaata atgatgttga aggccctgaa gcatctcacg
     4141 ggacacatgt tgcaggtatc ataggtgcca atagaaacaa caatctcggt atccggggta
     4201 ttgcagatca ggcgttgatc atgagtttgc gtgccgtgcc ggatggtgat gagcgagata
     4261 aggatattgc caactcgatc atctatgccg ttgacaatgg ggccaaagta atcaatatga
     4321 gcttcgggaa gggttattcc ccgggtaaaa aatatgttga tgcagccgtc aaatacgccg
     4381 ctaagaaaga tgtactttta gttcatgcag caggaaattc atcagccaac aacgacactg
     4441 agcctaattt tccgaatgca agatttgaga agaaaggact ttttggcaaa aaattcgcag
     4501 acaactggat cgaagtaggt gctttaagct ggaggccagg ggaggatatg gtcgccggat
     4561 tttctaacta tggcgcaaaa aatgttgacc tgttcgctcc cggagtcgat ctgtattcaa
     4621 ctgttcccaa tcaaagctac aaaaaggaaa gtggtacaag tatggctgct cctgtagtgg
     4681 caggtgtcgc agttttgctt cgctcctatt tccctacact atctgctaag caaaccaagg
     4741 aaatcatatt gcaatccgtc cataaggtga atgctatggt gaagaaacca ggcactgatg
     4801 aattggttcc tttcagcagc ttgagcgtca ccggtgggta tgtcgatgct tatgaagcga
     4861 tcaaactggc cgcaaaaaaa accggggtta aattagcaat gccttaataa ttcaagcata
     4921 taaggacttg aaacttggtc cttcctggaa tcagaaagcc ggcatgcccg gcttttttta
     4981 ttgaccattg gatcctaagc cactgaattc cctacctcaa caaagtaaaa tcaccttttg
     5041 ccaaagcttc cttaccatca atagtacggt acctgacata ataaacaaaa acaccagggt
     5101 tcatctttct gccatgaaag gtaccatccc atccattagc ttcttcgccc ggaacaaagt
     5161 tgcttaaact gaatacctga ttgccccatc tgtcatagat atgtaattcc ataatcatct
     5221 caaccttccg gttagccgga acaaagaata catcattacg cccatcagca ttgggcgaaa
     5281 aaatattggg cataaacaac ttcgtatcat tctcgacaaa tataacccga cttgcttcat
     5341 gcaggcaatc attcttgtcg cggataagaa cctgaattgt tgtcgtttca attggcttca
     5401 gccactcccc ttccggcttg ataaccgtcg gagtcccatt gagactgact cctatccatt
     5461 caattaactc ttgcggaaag tcaacttgaa cccaaatctc atctcctaac tggatggttg
     5521 tatcacgccc cagatgaaga cttcctgaaa caaaatcatc tacctggaaa tcaagcagga
     5581 atcgacaccc cttactatcc aacacccgaa gttggtgaag acctgcattt aaatcataga
     5641 attgtgtata ggaagtaaat gaggaattat ccaggctgta actgtaaggt ggagtccctc
     5701 caataggata atccagaatt aatgcacttc cattttcttg acaacctcca atcaaacgat
     5761 aagtaacggt atctataggg acagaattat caccgaccaa aaaagaaacc gtatcccggc
     5821 atcccgtaat ctgatcctga gatgaaaaat aataggtacc tgccttatca ataatcgccg
     5881 aatccaaact aatagaggta tgtataatat cattctgaaa gatccaatca gtgcgaattg
     5941 aagatgaagc cccgggatct acttttattt ccccgacctg agactcacag tccagataat
     6001 ttgttccgga taccaatatt tccggaagcg gatatacctt gatggtaaga ctcgcagtat
     6061 ctgaaggaca aacatcactt ccggcgacaa tcttatccaa catataatct cccggaaaaa
     6121 gctttttcgt actgatagta ttcgtctgtg gatcaaaagc tatcgaaaat ccggggtaat
     6181 caggtttttg agccttccag gctccatcat tcaaagaatt gatcagcaag gtatccagat
     6241 ctattaaata atcctcgcca ttacatatgt ccatcaaata atcgtcaccg gcataaaaac
     6301 gaggaacaac ctgcaactca aacaaaatgg tatctgcaca agcaccctcc caatccgcta
     6361 cagtctgcaa taggatagaa tgtacaccga caaactggtc attaataagg agattcgatc
     6421 caccaatggc ctctttgctc ccggaagtaa ggtcaaacca ttgtaccgga acattaactc
     6481 cggccaatga atccagattc aaagacccat aattactgca aatcctgtag gtatccttca
     6541 gtttgttcag cgcaggtctg ttgcaggaac aatcaaccac cttgaataca gtcgcatatg
     6601 taacatcctt acaaggcagt acagctgtgt tcgttctaaa attaaccttg taagtcccga
     6661 cagctacatg cgacatatct atcgaatcat tggaaatggt aatacctgac aatgaactca
     6721 gattccaatt tccgctcctt tctccggata caatcaaatc tggaaggaaa taaaccggct
     6781 ttccatccct gttgtaagta ttacatagag taattgtatc aggaagcaat gactccggca
     6841 tattttcaat cctgacatca agcgtatccg attgcagatc gttacaggtt ctgtattgcg
     6901 ttttcaagga atcaatatac actccacccg atgcattaaa atccagatct atataattga
     6961 gtccgatatc aaccggcata tgaaaacctt gaccctcaaa agaataatac aattcgccgg
     7021 cgtgattcac aatttccacc ggaatacggt aagtacctga tccgcagaat gccgtatcct
     7081 ttactataaa cttagggggc tcctgtctgt aaaatttaag gctgccaaac aaatcacatc
     7141 ctgcaatact cccactgtat ttcaacaccg ctgaattctt tcccaccaga atcgatcccg
     7201 ccacaagtga tccatctcca ttaacggaaa atattgaatt cccttgtata tccacccagg
     7261 tcagattctg aaattgactt tttacttcaa tgtggaacaa tccattgcaa gaaattgaat
     7321 cggtaagaat atcaaaaacc ggtttctgac cgaccgtgac atcctgacaa agcgtgtcgg
     7381 atgtgccaca ttcattcgtc gcccatacac agaccttgaa agttcccgtt tgatgccacg
     7441 tcacaaaaag cgtatcccct atcctcctga aatctgatgt cccggtaatc gaccaatgcg
     7501 cttcatccct caacgtatcg gcagataata aaactttaag ggtatattct gaacaaggag
     7561 aaaaagggag tatcgtaggc ctctctggcg gaaaaacctg tgattcaact tttttaggga
     7621 tgagatattc acattgaagg ttgccataca ggatattcat ataaagaaaa tataataatt
     7681 gaaaaattag atgaatcatc taataaacat atagatttat cacttagaaa atctttaaga
     7741 aatgaattat atactgataa tattcttata gataatgatg aaacagagga tttagataat
     7801 aatacaatct ataaagaaga tgttattatt tcaaaagaaa gaaagatgtc agtatcaagt
     7861 aataataata aaaaagaata tattgaatta tagttagaat tacatctttg gtt
//
LOCUS       DAOQMC010000103         7903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3698_length_7903_cov_4.666667, whole genome shotgun sequence.
ACCESSION   DAOQMC010000103 DAOQMC010000000
VERSION     DAOQMC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3698_length_7903_cov_4.666667"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2788)
                     /locus_tag="PKV31_05435"
     CDS             complement(<1..2788)
                     /locus_tag="PKV31_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92330.1"
                     /translation="MKNTTTLGLNRGPSHRLLNTRMSRGATFLAIFIMVLYASGIGYS
                     QYYNVNQNCTTSFDDISGTGTQLTVNGDGHTFNSGLPFNFQFFGVNYTTPSIKISMDG
                     YIMWNTTTGDLVNDNLPLPVNDPDINTYGAIYPFWDFSFTDISTPPAGVYVETKGIAP
                     NRVFMVQWEGVQRVIDETTPTYSYGKVTYQVLFYETTNEIKFVYEDTFYGSTIADGSE
                     NNNGASSTIGLQKGGSPIRIYQTSFDDPTLLQGSSCISYLPAATTSGCTMACNTTNIG
                     IPANCRPTLIARDFLTTSYDCATSFIIQLLQTPTGPILEQGVDSLVADGISIGGNPYP
                     LVNQNYVINIIENITGGSPNSCWNWHTFQDNVAPEIVCRPADTVFCYEPLVLYSLTGI
                     TDCSGPLTAHVIDSTYTPYSCSIDPYLLGRITRRYFLTDASGNRSDTCEDVLFLAPPS
                     KDSIQFPPHFGTLQCDDNFAADAYGRPLPSVTGVPTIRNTSINLYPSTLGLLCNMTTS
                     YSDHELPMGCGIKIMRRWVSYFWTCAGEDSIAHIQEIIISDTVPPVVTAPADVTITAG
                     QNCRASYTVPPATITDNCNHLGNVSVIHPNGVTNQNGGFTITNLSIGTYNIIYSVNDG
                     CGHIVRDTTKLTVQDLTAPVAICKEAIVSLDNTGTGRMFASSINNGSHDNGCGPVTLK
                     VRRMNPDCNGAPDQTTWADYIDFYCCDLPNNPIQVILQVTDAGGLTNTCMTTVTVQNK
                     NTPIIAYPLPNIIVPCGTSFDVNNLALTFGEYVTNPADRQKIIIDGVDKGWDGLITGV
                     CTATVTETAPTINIPQCGFGTITRHFLITDGGSFQQTVSQTITINYDGVELTTADFNA
                     PLDYVVTSGICTPPDLANADLGSPYVPTLKSPLNGCHNIMINKEDIVFTNASNACFKI
                     VRTWTIIDWCLAAAK"
     gene            3233..3841
                     /gene="yihA"
                     /locus_tag="PKV31_05440"
     CDS             3233..3841
                     /gene="yihA"
                     /locus_tag="PKV31_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNB92331.1"
                     /translation="MAGQLNIHFKGSFSDVKACPVTTLPEYAFIGRSNVGKSSLVNML
                     SGKTHIAKVSNTPGKTQLINLFEINEQWNIADLPGYGYAKTSRVNRTAWGKMVTNYLL
                     QRENLVLLFVLIDSRLPAQKIDLDFMEFCGKKQIPFSIIYTKSDKSSTNQLKKQIAEI
                     QKELLRHWNALPDQFISSSQNKSGRDEILDYIYGINEQIVRK"
     gene            3819..5558
                     /locus_tag="PKV31_05445"
     CDS             3819..5558
                     /locus_tag="PKV31_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB92332.1"
                     /translation="MNRLSESRPLEDPISREKDQIYPPLIDDINQWPINKLTENRSAF
                     VKKLEAYSFNTISRKSTDFIKDLIQKTIFQEKTRIKEEPWRIDPPNDRVFWNRIRKKM
                     ADSDIDSSHQKEENVNESLKLIIHRYAEEIVGTFNTKTYRLIRKILTILFKRLLNKGI
                     KVAPSNKVDKVLLDKLKIYGPIEKIRMLSSKGTVVTLPTHSSNLDSVLIGYTMDYKLG
                     LTGSAYGAGLNLYNFGPAAYFMNRLGAYRVDRRKKSVIYLTTLAGMSKLAIQAGTPSL
                     FFPGGTRSRSGGLEDKLKLGLLNSVIEAQRSIYEEGRNDKVYVVPLVLSYHFVLEAKN
                     LIEDFLKKTGKERYVKSRNEFTSFRSWTKFLYQFISKSTTISLSFGEPMDCFGNFLND
                     EGESVDPRGKVIDTRDYFMYEGKLTSNHQRESEYTKNLGNIVAERYKAENIILSSHLV
                     SYVAFQLLKAANPNLDLYSLLSLPEDEFFFSREQFLEQSGIVVEMLKGKALERLLKLP
                     EIFDRDILTLVLDGIDQIGTFHSSRPITIHKNGDFYSQDFRLLYFYNNRLESYGYESE
                     IAWNNLPVVYQID"
     gene            5559..6104
                     /locus_tag="PKV31_05450"
     CDS             5559..6104
                     /locus_tag="PKV31_05450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB92333.1"
                     /translation="MHYILFDLEATCWLGRPPIGNNEIIEIGAYKVNGYGEIVDHFNQ
                     YIKPISNPTLSGFCKKLTGIQQHNVDTAFKFDKVIGDFMEWFGYEDNDEYHLISWGEN
                     DRQLLFQDCTLHDVEDDWIRFWVNLKPAYQRLTGMLKQVGLKKALERETIEFEGKQHR
                     AITDAYNLTKLFIKHFDDWEL"
     gene            6261..6902
                     /locus_tag="PKV31_05455"
     CDS             6261..6902
                     /locus_tag="PKV31_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNB92334.1"
                     /translation="MLIFLTSGFSQYVRMGVSSVKASSPEAQQQIEATMKSMKIESFA
                     TPDKVRSDVNMMGGMVVMSTFTNPNDDSFVMYMDMMGQKMKVVPTPEELKSIKEEGDN
                     NSKDMKITEVPGDSKTILGFKCKKINVSNGDMNVVMYVTPDLKMKANRIQGMEGLKID
                     GYPLEYTVEAAGTKMTFTADKYEPTFDDGKMKEPSGKYKEMTFTEFKKQMPGM"
     gene            7250..7864
                     /locus_tag="PKV31_05460"
     CDS             7250..7864
                     /locus_tag="PKV31_05460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNB92335.1"
                     /translation="MNKTVWVIRHGETELNASGIVQGQGMDAPLNIKGFEQAESFWKV
                     YSEEPFDLVLYSNLIRSQQSVQRFLTSGVQGVELADLREISWGVNEGKQPTPDVLQRY
                     HAVIDAWQSGDFAAAVEGGESAIQLAKRAQTCITYFNEINRKNILVCTHGRTIRVLAC
                     LMLNRPLTAMEDFHHANLGLYRFDMDGDGWQCTLYNDIRHLQGL"
BASE COUNT         2361 a         1515 c         1742 g         2285 t
ORIGIN      
        1 ctttggctgc tgcaagacac cagtcgatga tggtccaggt acgtactatc ttgaaacatg
       61 cattggaagc attagtaaat acgatgtctt ctttgtttat cattatgttg tgacatccat
      121 tcaacggtga tttcaaggta ggtacatacg gactgccgag atctgcattg gcaaggtcag
      181 gtggtgtgca aatgccactt gtgacaacat agtccaatgg tgcattgaaa tcagctgtag
      241 taagttctac accgtcatag ttgatggtaa tggtttgtga tactgtttgt tggaatgagc
      301 ctccgtctgt tataaggaag tgtcgggtga tggttccgaa tccacattgc gggatattga
      361 tggtaggtgc tgtttctgtt actgttgctg tgcaaacgcc cgtgataagc ccatcccagc
      421 ctttatctac gccatcaata atgatttttt ggcgatcagc cggattggtt acatactcac
      481 caaaagtcag tgccagatta ttgacatcga atgaagtgcc acaaggaaca ataatgtttg
      541 gaaggggata ggcaatgatt ggcgtattct tattctgaac cgttactgtt gtcatacaag
      601 tattggtcaa gccacctgca tccgtaacct gaagaatgac ctggatcgga ttgttcggta
      661 agtcacagca gtaaaagtca atgtaatccg cccatgtagt ttgatcaggc gcaccattgc
      721 aatccggatt catcctcctg accttgagag tcaccgggcc gcagccatta tcatgacttc
      781 cattgttgat ggaagaggca aacatccttc ctgttcctgt gttgtccaga gagacgatag
      841 cctccttgca gattgctacc ggtgctgtaa ggtcctgaac ggtcaatttt gtggtatccc
      901 gaacaatatg tccacagccg tcattgaccg agtaaatgat gttgtatgtg cctattgaaa
      961 gatttgtaat cgtaaatcct ccgttttggt tagttactcc attggggtgg atcacagata
     1021 catttccaag atggttgcaa ttgtcggtaa tcgtagcagg aggtacagta taggatgccc
     1081 tgcagttctg acctgcagtg atggtaacat cggcaggtgc tgtgaccacg ggtggaaccg
     1141 tatcactgat aatgatttcc tgaatatgag cgatagaatc ttctcctgca catgtccaga
     1201 agtatgaaac ccaccttctc atgatcttta taccacaacc catgggtagt tcatgatccg
     1261 aatatgatgt cgtcatgttg cacaatagcc ccagagtact gggatagaga ttgatacttg
     1321 tatttcttat tgtcggaact cccgtaacac ttggtaaagg acgtccataa gcatccgctg
     1381 cgaaattgtc atcacattgt aaagttccaa aatgaggagg aaactgaata ctgtctttac
     1441 ttggaggtgc cagaaacaag acatcctcac atgtatcaga cctgttacct gatgcatctg
     1501 tcaggaaata tcttctggtt atcctcccga gcagataagg atcaatactg caggagtagg
     1561 gagtataggt cgaatcaatg acatgtgctg tcagcggccc ggaacaatcc gtaattccgg
     1621 taagtgagta caatacaagt ggctcataac agaatacggt atctgccggt ctgcaaacaa
     1681 tttccggagc tacgttgtct tggaatgtat gccagttcca acaagaattt ggagatcctc
     1741 cggtaatatt ttctataata tttatgacgt aattttgatt gaccaacgga tatgggtttc
     1801 cccctatgga tattccgtca gcaaccagtg agtctacacc ttgttcaagg attggtccgg
     1861 tgggcgtttg taataattgt ataatgaatg acgttgcaca atcgtatgag gttgttaaga
     1921 aatcccgggc tatcagcgtt ggtctgcaat tggccgggat gcctatgtta gtagtattac
     1981 atgccattgt acaccctgaa gttgttgcag caggcaggta ggaaatacag gatgagccct
     2041 gtagcaatgt aggatcatca aatgatgtct ggtatattct tattggtgaa ccaccttttt
     2101 gtagtccgat ggtagaactt gcaccgttgt tgttttcact accatctgct atcgtacttc
     2161 catagaaagt gtcttcatag acaaatttaa tctcattggt ggtctcatag aacaacacct
     2221 gataggttac tttcccgtaa ctgtaagtgg gagtggtttc atctatcact ctttgtacac
     2281 cttcccattg taccataaat acacgattgg gcgctatccc ttttgtctct acatagactc
     2341 ctgccggagg tgtcgatatg tcggtaaagc taaagtccca aaatggataa atagcgccat
     2401 aagtgttgat atccggatca ttgacaggta ggggaaggtt gtcattgacc agatctcctg
     2461 tggtcgtgtt ccacatgata tacccgtcca tacttatttt gatggaaggc gtcgtgtaat
     2521 tcacaccaaa gaactggaaa ttgaatggta agccgctgtt gaaagtatga ccatctccat
     2581 tgacagtgag ttgcgtgcct gttccggaaa tatcgtcaaa tgatgtcgta cagttctgat
     2641 tgacattgta gtattgactg tatccgatgc cggacgcata tagcaccata atgaatatgg
     2701 caagaaatgt tgcaccgcga ctcatccttg tattgagtag tcggtgggaa ggtcctcggt
     2761 tgaggcctaa agttgtagtg ttcttcataa gactaaaata tggttagaaa atataaaccc
     2821 ggaccgccat gcacagcgcg tccatttctt tatacagtta ttggggagaa gtggcattcc
     2881 aatctgcttg aaatgccatt tgaatgacaa agatacacat taagatattt tctaatgtgt
     2941 ttttatatta aatatttttg taaaatataa ataatttatt tatagatata tataaatatc
     3001 agatatgtgt aatttaatag cttgaaaatc aacttcttta tatttgattg atctatcttc
     3061 ctttactgcg gctgacaatt tttatttttt aaagattggt ggtctttagt ttttaactaa
     3121 taaaatattt catagtaaca atcctcaatt cttagattgg aacaccatca accactcctt
     3181 tagcaatgat agtttgattt tattatcttt gtgccctgaa aatccaaata tcttggccgg
     3241 acaacttaat attcatttta aagggagttt ttctgatgtt aaagcatgcc ctgtaacaac
     3301 tttgccggag tatgcattca taggaaggtc caacgtcgga aaatcttctc tggtcaatat
     3361 gttgtcagga aagacccata tagcaaaagt gtcaaatact cctggaaaaa cccaactcat
     3421 taatctgttt gaaatcaacg agcagtggaa tattgcagac ttgcccggat acggttatgc
     3481 aaagacatcc cgggtcaaca ggactgcatg gggcaagatg gtgactaact atttgctgca
     3541 aagagaaaac ctcgttctgc tatttgttct gatcgacagt cgattgcctg cgcaaaaaat
     3601 tgatcttgac tttatggaat tttgcggaaa aaaacagatt ccgttttcga ttatctatac
     3661 caaaagtgat aagtcttcca ccaatcagtt gaaaaaacaa attgctgaaa ttcagaaaga
     3721 attattgcgt cattggaatg cattgcccga tcagtttatc tcctccagcc aaaataaatc
     3781 cggccgcgat gagattcttg actatattta tggtatcaat gaacagattg tcagaaagta
     3841 gaccgcttga agatcccatc tccagagaga aggaccagat ttatcctccg ttgatagacg
     3901 atatcaatca atggccgatc aataaactga ctgaaaaccg tagtgctttt gtaaaaaagc
     3961 tggaggcata ctcctttaat actataagtc gaaaatcgac agacttcatc aaagacctca
     4021 tccaaaaaac aattttccag gagaaaacca gaatcaagga agaaccctgg cgaattgatc
     4081 ctcccaatga ccgggtattc tggaatcgaa tccgcaaaaa aatggccgat tcggatatcg
     4141 attccagtca tcagaaagaa gaaaatgtca atgagagcct gaaacttatc atacatcgtt
     4201 atgcagaaga aatcgtaggc acattcaata ccaagactta cagactgatc aggaagattc
     4261 tgaccatcct tttcaaaaga ttactcaaca aaggaatcaa ggtcgcccct tccaataagg
     4321 tagataaggt tctgctcgat aaactcaaaa tatacggacc cattgaaaag atcagaatgc
     4381 tttcctcgaa gggcacagtt gtgacccttc ctacacattc gagcaacctg gattctgttc
     4441 tcattggtta tacaatggac tacaagctcg gtttgacagg ttccgcatat ggtgccggac
     4501 tcaaccttta taattttggc ccggctgcct actttatgaa cagactcggt gcctatcgtg
     4561 tcgatcgaag aaagaaaagt gtcatctatc taaccacctt agctgggatg tctaaattgg
     4621 ctatccaggc aggtacgcca tctttattct ttccaggtgg cacccgatcc cgctcaggcg
     4681 gacttgaaga taagttgaaa ctgggtttgc tcaactcggt tattgaagcc cagcgctcca
     4741 tctacgaaga gggcaggaat gataaagtgt atgttgtgcc cttggtactt tcttatcact
     4801 ttgttttaga agccaaaaac ctcatcgagg attttcttaa gaagacaggg aaagagcgtt
     4861 atgttaagtc gcgtaatgaa ttcacttcct ttcgcagctg gactaagttc ctgtatcagt
     4921 ttatctcaaa aagtaccaca atctccctgt catttggtga gccaatggat tgcttcggta
     4981 atttcctcaa tgatgaagga gaaagtgtgg acccacgagg taaggtcata gatacaaggg
     5041 attattttat gtacgagggt aagttgacgt ccaaccatca aagggaatcg gaatatacta
     5101 aaaacctggg aaatattgtg gcagaaaggt ataaggcaga aaatattatt ctgtccagtc
     5161 accttgtatc ctacgtcgcc ttccaattgc tgaaggctgc caatccaaat cttgacttgt
     5221 acagtctgct ctctttgccg gaagatgagt ttttcttctc cagagaacaa ttcctggaac
     5281 aatcgggtat cgtagtcgaa atgctcaagg gtaaggcact cgaaagactg ttgaagttgc
     5341 cggaaatttt tgaccgggat atactgactt tggtgcttga tggtatagac cagattggca
     5401 cctttcattc gagtcgccct atcacgatac acaaaaatgg agatttctac agtcaggatt
     5461 tccgattgct ttatttttat aataaccgcc ttgaatcata cggatatgag tcggagattg
     5521 cctggaacaa tctgccggtg gtgtaccaaa ttgactaaat gcattacata ctgttcgatc
     5581 ttgaagcgac atgctggctg ggaaggccac caatcggcaa caatgaaatc attgaaatcg
     5641 gagcttataa agtcaatggc tatggtgaga ttgtcgatca cttcaatcag tatatcaaac
     5701 caatctcaaa tcctacttta tccggatttt gcaagaaact aacaggtatc cagcagcata
     5761 atgtggatac cgcatttaag tttgacaaag ttatcggaga ttttatggaa tggtttggtt
     5821 atgaggacaa tgatgaatat catttgatat cctggggtga gaatgacagg caacttttat
     5881 ttcaggattg caccctgcat gatgtcgagg acgattggat aaggttttgg gtcaatctca
     5941 aacctgcata tcaacgcctg acgggaatgc tcaaacaagt tggactaaaa aaagcactcg
     6001 aacgcgagac cattgaattt gaagggaaac aacatagagc cattaccgat gcttataatt
     6061 taacaaaact ttttatcaag cattttgatg attgggaact ataatgctat atatttgctt
     6121 ttgactaaaa tccgtttctt atttggatta agcatcggga ttcgatgaat atttttctaa
     6181 aaatcaacaa aattaataac cacttattta aaaatcaaaa ggtaataaat atgaaaaaaa
     6241 ttaatttaat tgtttgcttt atgctgatat tcctgacaag tggattcagt caatatgtca
     6301 gaatgggtgt cagctctgtt aaggcttcca gccccgaagc tcagcaacag atagaagcga
     6361 caatgaaatc aatgaaaata gaaagttttg caactcctga taaagtaaga tctgatgtca
     6421 atatgatggg aggcatggtt gtcatgagca cttttaccaa tcccaatgac gacagttttg
     6481 taatgtatat ggatatgatg ggccagaaga tgaaggttgt gcctacgcct gaagaactca
     6541 agagtatcaa ggaggaagga gataataaca gtaaggatat gaaaatcacg gaagttcccg
     6601 gagattcaaa gaccatcctt ggattcaaat gtaagaagat caacgtaagc aacggtgata
     6661 tgaatgttgt catgtatgtg acacctgact taaagatgaa ggccaataga atccagggaa
     6721 tggaaggtct gaagatcgat ggttacccat tggaatatac ggttgaagct gcagggacaa
     6781 aaatgacttt tacagcagat aaatatgaac cgacttttga tgatggcaaa atgaaggagc
     6841 cttccggaaa atataaggaa atgaccttta cagagttcaa aaaacaaatg ccgggcatgt
     6901 aaaacataac acgaaagcgg cttgaaggcc gcttttttta ttatgaccgg tagggtttca
     6961 gagtcgtgat agctataaca tttgctaatt ttatttgtcg gacagctcag tgccttaaag
     7021 agcattttct gggatgaccg gttataagtg cgatggttta tttgtgatgg gataagatca
     7081 aacggggcta ttaaccacgg tggatttctt agaattgcgc tcaatagagt cataatgcct
     7141 gagagtcata attattttaa acttgctcat ttttagatca ccaatcactt atttttttca
     7201 gagttctgtt aaaaatactt aatttaggag caaaaacagg gaaattatct tgaataaaac
     7261 agtttgggta atccgacatg gagaaacaga gctgaacgcc agcggaatag ttcaggggca
     7321 aggaatggat gcacctttga atataaaagg ttttgaacag gccgaaagct tttggaaagt
     7381 atattcggaa gagccatttg atctggtact ctattccaat cttattcggt ctcaacagtc
     7441 cgtacagcga tttctgacct ctggtgtgca gggtgtggaa ctggctgatt tgagggagat
     7501 aagctggggg gtgaatgaag gtaagcaacc gacacctgac gtcttacaac gttatcatgc
     7561 tgtcatagat gcctggcagt caggtgattt cgctgctgcc gtggaaggtg gcgaatcggc
     7621 tattcaactg gccaaaagag cacaaacatg tatcacctat ttcaacgaaa tcaacagaaa
     7681 aaatatcctg gtttgtactc atggaagaac aatcagagta ttagcctgtt taatgctgaa
     7741 tagacctttg actgctatgg aggattttca tcatgcaaac ctgggccttt atagattcga
     7801 tatggatggt gatggttggc aatgtacatt gtacaatgat atcaggcatt tacaaggtct
     7861 gtagtaagcc cggggaatcg tcggaaggga ttggtttcat ggt
//
LOCUS       DAOQMC010000104         7894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3704_length_7894_cov_5.378365, whole genome shotgun sequence.
ACCESSION   DAOQMC010000104 DAOQMC010000000
VERSION     DAOQMC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7894
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3704_length_7894_cov_5.378365"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..729)
                     /locus_tag="PKV31_05465"
     CDS             complement(13..729)
                     /locus_tag="PKV31_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB92336.1"
                     /translation="MKFPIQVEQKNCLNCAFKGHSLFSILSDDSLDHLNAHKSCTIFK
                     KGQYLFTQHSRPMGLFCVYSGKIKLSTAGPDGKEQIIRLAKDGDVIGYRAIITDENYR
                     LSAIALEESSVCLIEKEFFLDLAMSEPRLNRQIFKLISDDLKRAEEQIVSLSQKSVRE
                     RTAEALLFLKATYGFEDDNQTLNIRLSREELADYVGTTTESVIRVLSDFKTSGLIDIA
                     ARKIIIIDLGLLIKTANMIP"
     gene            complement(917..1873)
                     /locus_tag="PKV31_05470"
     CDS             complement(917..1873)
                     /locus_tag="PKV31_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNB92337.1"
                     /translation="MPKSSVLHITHDSFVKLVLSNPAAGQDFLNYFLPESLKGNLDIE
                     KCVPSELSYLTESMRNRHTDLIFDVPIKGSGEGIRIAILIEHKSYKDEAVIFQLLEYL
                     ALGYRKQSREKKGFRLIIPLLYYHGKQEWSPAGLSSYFIDIPGDLKPYLPEFSRIYID
                     LKNYSIDDIERLNNSLLRTALYIQLLRLQNMENKTILSRAFTTFEYGQYRNYFSAIFV
                     YAIKYLDLEKEEFEILVDEFPEEVKPEAMTLANRLKREGMEEGIEIGKKQGIEKGIEK
                     GIELSQVSFVFKSYENGLDALTIAKIVGITEDRVREILNIER"
     gene            2027..3772
                     /gene="recD"
                     /locus_tag="PKV31_05475"
     CDS             2027..3772
                     /gene="recD"
                     /locus_tag="PKV31_05475"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNB92338.1"
                     /translation="MDISSINSADAFAGFFAKDDLFPWLRTLIEQMVLGNICIDIDAF
                     SESQYHNLSILSDHILVGPPQENKPFVIHNGRLYINRYFQYEKSLIEKIRQLCELVAA
                     DRDFDFLHDNKDFIVRHLFPPSGYVKGADWQLVACLTAFLSRFTIISGGPGTGKTTTV
                     AKLLYLLLKQTPDAKIGVCAPTGKASAHLDESFARAGDKLAGSEFNDELVQKIADLKA
                     LTLHSMLGYIHNSIHFKHHEGNPLDFDILIVDECSMIDITMFFKLFKAIDPGRTRVVL
                     LGDKNQLASVEAGSIFRDLCNDTAPMNIFSKVRTDFINGFIEEPLDCMSGEVTHPLFE
                     RMIELQTSFRFSDDEGIGKFSKAVLNNDQVVIKEFLENKDDRVKFLCPNDVESALKSA
                     AQRLLQVNGGYVGQTGVGEALNRLVKSIVLCAVKEGKTGVLAVNRMIASEVSHNNERF
                     YPFLPVMVASNQHSEGIYNGDMGLILEVKGEVNAYFPKGNGENLKLNPAQIREWEPAY
                     AMTIHKSQGSEFDEVLIILPENRENILLTRELLYTAITRAKEKVTIVGFSEIIRAIAE
                     QSVKRVSGIKDHFKS"
     gene            3777..6941
                     /locus_tag="PKV31_05480"
     CDS             3777..6941
                     /locus_tag="PKV31_05480"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNB92339.1"
                     /translation="MGIHIPISNDITKLADAMIEDIGLYRFDVFQPVYIVTQTKGMNS
                     WLKYRMSEQHGVAANLMFLTPEEIVYEVYKILGGSYKDKIDRKDMDWLIYAALSDEAF
                     KEKFPERASYFYNEEGRDKMKQWELAAKMGDLFDQYQIYRSEVIAQWKVNELESVEEK
                     SRWQAYLWKKIDSIAGDKIIDFNAIKVDILEKLKTVQNQERLVRNIPVLHLFGLSIIT
                     KYHLEIIHAMSEHIDIRWYANNPAPDYYWFEDEPQKVLDKWKAKNRRIEHITVGHPLL
                     TDWGKVIQSIFRLISGHEEFINAITDLPLKQNNGTTLLASLQQSIQHNSVDRPDFDTG
                     LFDDDTISITSNYSKRREVETLYNYILDTIATKRHGDILDREIIVMATDINAYAPYIR
                     AVMDNAPYRFKYNIADEALANVNSPVAALISLLEFDGKLFGAEEVLGLLTHKYIREKF
                     RINDLELIRNTIVKANARFGIENDYSTPVNDTFIVSWRYALEKIFYGICLPGEIRIDN
                     EHPFYTVETTDTVAGIQEISAFLAFFEALKTHVHERKNPKPLREWRKFIEQVLEDMFA
                     IPDDEKNEDYVKKIEKLIRFENLDDGLKDEEIPYDVFSKRFLTGLKVETTSTRFMSRG
                     ITFCSPLPFRSLPFKLVCMLGLNHGEYPRLERRSDFDLIASNRKLGDRNLKENDKHLF
                     LESILSAEKALYLSYIGRSIKDNEILPPSILIDELLDYLQMGNVEVDVRKKLVSEHPF
                     YSFSTKYNKDSGNLLPNYFIQLAKEWPIPYNDNREVPEIKEYDINTVWKFFEKNIQYY
                     YNNVLGVYFEREDGDIKDTENFELDSLEEWTVKESLLDSRLKGVEQALEEKLYLSGQL
                     PLKNVGLGLVDEIDEVISPLVDDVKNDFPDNFDPKIVSIEITYDDVRYFGEIKNVIDK
                     KIISFHHGSSGGEAKRRARLAFQYLFAVVCNVDITGGALYTLNGKHTLKQLGVDEARA
                     RLGNILTFFHENKERLIGYSAEQYFQSLKEDDSFDKIKNFFFIDRNYRDDYYRHVMLN
                     HDINIHRVWDMHVILQEILLDNAY"
     gene            6955..>7894
                     /locus_tag="PKV31_05485"
     CDS             6955..>7894
                     /locus_tag="PKV31_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB92340.1"
                     /translation="MEAINFKVNEVPLSGTNLIEASAGTGKTFSIGILVLRTIVEKNI
                     PIDNQLIVTFTNYAVAELQDRIRKFLSQAIEIASASEQELVLIADRFEQPIVEICERF
                     EDKTELITRLRQSQLLLDEASIFTIHGFCQRMLNEFAFESGQNFQTELQADVSYLIER
                     VTNEFWRNEITTLDKDLFESKDLKKVRAYFKEILKQGLNNTQFDVNVSSPIKDNGKIS
                     DIIECYKGYLKELETEWERLECFIAEDNTRLKVDVESNSNTRKKYSDLLGQPRLMLQV
                     TFDYFKANKAAGYFDKFSSPYWNDVKSLVERENNLNN"
BASE COUNT         2440 a         1390 c         1692 g         2372 t
ORIGIN      
        1 atcgcattca gcctaaggaa tcatattggc agtcttaatc agcaggccca aatcaatgat
       61 aattattttt ctagcggcga tatcaattaa gccacttgtt ttgaaatctg aaagaaccct
      121 tattacgctt tcagtagtcg tacctacata atcggccaat tcttcccgac ttaggcggat
      181 attgagtgtt tggttatcat cttcaaagcc ataagttgct tttaagaata aaagtgcctc
      241 agcagtacgc tcgcgcacac ttttttgtga caaggaaaca atttgttcct ccgctctctt
      301 taaatcatcg ctgattaatt tgaatatttg cctgttcagc ctgggttcac tcatggctaa
      361 atcgagaaaa aattccttct cgatcaggca cactgaactt tcttccaatg ctatagcaga
      421 aagtctgtaa ttttcatccg taatgattgc acgatagcct attacatcgc catccttagc
      481 cagccggata atctgttcct ttccatcagg accggctgta gagagcttta tcttacctga
      541 gtatacacaa aatagaccca ttggtcgact gtgttgggtg aaaagatatt gacctttctt
      601 gaaaatagtg caagacttgt gggcgttgag atgatcgagc gaatcgtccg ataaaatcga
      661 aaacagtgag tggcccttga aagcgcaatt gagacaattt ttttgttcta cctgaatcgg
      721 gaatttcatg atcaataaaa aatcaaatat atgaaatatt atcgaaatat tttcagaatg
      781 aattttttat ccggaatcat ctaagttgcc tctcaatttc cataaggcct tggaatgccg
      841 aaatcaagta tcatacgatt ggatataaat ggagtagttg gcaaagatca attcattgga
      901 taaagattcc gattgattat ctttcaatgt tgagaatttc tctgacacgg tcttctgtaa
      961 tgccgacaat tttagcgatg gtcaatgcat cgagtccgtt ttcataactc ttgaaaacga
     1021 aagaaacttg tgatagttca atcccttttt caattccttt ttcgatgcct tgttttttgc
     1081 ctatttcaat accttcctcc atcccttcgc gcttcagtcg atttgcgagg gtcatagctt
     1141 cgggttttac ctcttcggga aactcatcaa ccaaaatttc gaattcttct ttttccaagt
     1201 ccagatattt aatcgcataa acaaatattg cactaaaata gttcctgtac tgaccgtatt
     1261 caaaggttgt aaatgccctg ctcaatatag ttttgttttc catattttgc aatcttaata
     1321 gttgaatgta tagagcggtt cgtaacaatg aattgttcaa tcgttcaata tcatcaatgg
     1381 aataattttt gagatcgatg tatatccggc taaattcggg taggtatggc ttcaaatcgc
     1441 cgggtatatc tataaaatac gaactcaaac cggcggggct ccattcttgt ttcccatgat
     1501 aatagagtag tggaataatc aacctgaaac cttttttttc cctgctttgc ttcctgtaac
     1561 ctaaagctaa atattccaac aattggaaaa ttactgcttc gtccttatac gatttgtgtt
     1621 cgattaatat tgcaattcgg attccttcac cggagccttt tatgggaaca tcaaaaatca
     1681 ggtcagtgtg gcgattacgc attgattcgg tgagataaga caattcactc ggtacacatt
     1741 tctctatatc caaattcccc ttaagacttt ctggtagaaa ataatttaaa aagtcttgtc
     1801 ctgcagctgg attggaaagt accaatttga caaaggaatc gtgggtgatg tgtaaaacgg
     1861 atgatttagg catgatttta aatttcgata aaaataagaa ttaatttcaa aaagcagaaa
     1921 ttggaataca taaaatttag atatgtagaa aatttaaata tcttcttgtt aatttctaca
     1981 tatatattta tttatactat cttgcaaatc cataaattta acttggatgg acatcagttc
     2041 aataaactca gccgatgctt ttgccggatt ttttgcaaaa gatgatcttt tcccatggtt
     2101 aaggactttg attgaacaaa tggtattggg aaatatatgt atagatattg atgcattttc
     2161 cgaaagtcaa tatcacaatt tatctattct gtcagatcat atactggtcg gtcctcctca
     2221 ggagaacaag cccttcgtaa tccacaatgg aagactgtat atcaaccggt atttccaata
     2281 tgaaaaatca cttatagaaa aaatcaggca attatgtgag cttgtggcag ctgacaggga
     2341 ttttgatttt ttacacgata ataaggattt tatcgtcaga cacttgtttc ctccttccgg
     2401 ctacgtaaag ggagcagatt ggcagttggt agcatgctta actgcatttt taagtcgatt
     2461 tacaattatt tcagggggtc cgggaactgg taaaactaca acagtcgcca agttactcta
     2521 cctcttactt aaacagactc cggatgctaa aataggtgtt tgtgcaccta cagggaaagc
     2581 aagcgcacac cttgatgaaa gctttgccag ggccggagat aagttggcgg gaagtgaatt
     2641 caatgatgaa ttggttcaaa agattgctga tctcaaggcg ctgacattgc attcgatgtt
     2701 gggatatatc cacaacagca ttcacttcaa acatcatgag ggcaacccat tggattttga
     2761 tatcctcatc gttgatgaat gttccatgat tgatatcacg atgtttttca agctgttcaa
     2821 ggcgattgat cccggcagaa ccagagtggt gcttttagga gataagaacc agttggcttc
     2881 ggttgaagca ggcagtatct ttagggacct ctgcaacgat actgcaccga tgaatatctt
     2941 ctctaaggta aggactgatt ttatcaatgg ttttattgaa gagcccttgg attgtatgtc
     3001 aggagaagtt acacatcctc tttttgaaag aatgattgag ctacagacca gtttccgttt
     3061 tagtgacgat gaaggaatcg ggaagttcag caaggctgtt ttgaacaacg atcaggttgt
     3121 tattaaagaa ttccttgaaa ataaagatga tagggttaaa tttctgtgcc cgaatgatgt
     3181 ggaaagcgct ttgaagtcgg ctgctcagag gcttttgcag gtcaatggtg gatatgtcgg
     3241 acagaccgga gtcggcgagg cattaaatcg acttgtaaaa agtatagtgc tgtgtgcagt
     3301 taaagaaggc aagactggcg tattggcggt aaatcgaatg attgcctctg aggtatcaca
     3361 caataatgag cgattttatc ccttcctccc ggtaatggtt gcttccaatc agcattctga
     3421 aggtatttat aatggtgata tgggtctgat tttagaagta aaaggtgagg tcaatgctta
     3481 ttttcctaaa ggtaacggcg agaatctaaa gctcaatccg gcccagatcc gtgaatggga
     3541 accggcttac gctatgacca ttcacaaaag ccagggctct gaattcgatg aagtcttgat
     3601 catcctgcct gagaacagag aaaacatttt actgacacgc gaattgctgt atacagccat
     3661 tacacgagca aaagaaaaag tcaccattgt aggctttagt gaaatcatcc gtgcaattgc
     3721 cgaacagtcc gtcaagcggg tatccggtat taaggaccat tttaaatcat aaagttatgg
     3781 gcatacacat acctatttca aatgatatta ccaagttggc cgatgctatg attgaggaca
     3841 tcggcctgta caggtttgat gtgttccaac cggtctatat cgtcactcaa acgaagggca
     3901 tgaacagttg gctcaagtac agaatgtctg aacagcatgg tgtggccgcc aacctgatgt
     3961 ttttgacacc ggaggaaata gtatacgaag tctataagat tctgggcggc agctataaag
     4021 ataaaattga ccggaaggac atggattggt tgatatatgc tgccttgtct gacgaagcat
     4081 ttaaagaaaa gttccctgaa agagcttcct acttttacaa tgaagaaggt agagataaga
     4141 tgaaacaatg ggaattagcg gctaagatgg gggatttgtt tgatcagtat cagatctacc
     4201 gcagtgaagt tattgcacaa tggaaggtaa atgaattgga aagcgtggaa gagaagtccc
     4261 gctggcaggc ctatttatgg aaaaagattg attcgattgc cggtgataag atcattgatt
     4321 ttaatgcaat aaaagtagat atcctggaaa aactcaaaac cgtacaaaat caggagcgtc
     4381 tggtcaggaa tattccggtt ttgcatcttt tcggtctttc catcattaca aaataccatt
     4441 tggagatcat ccatgcaatg agtgaacaca tagatattcg ttggtatgcg aataatccgg
     4501 cccccgatta ttattggttt gaagatgagc cacaaaaggt attggataaa tggaaggcga
     4561 aaaacaggag aatagaacat attaccgttg gacatccctt attgacagat tggggtaagg
     4621 ttattcaaag tatattcagg ttgatatccg gtcatgagga atttatcaat gcgattaccg
     4681 acctgccttt aaaacaaaac aatggaacca ctttattagc ttctcttcaa caatccatcc
     4741 agcacaattc ggttgatcga cccgattttg acaccggatt gtttgatgat gatacgatct
     4801 ccattacttc caattattcc aaaaggcgtg aagtcgagac attgtacaat tatattttgg
     4861 ataccatagc taccaaacgg catggtgata ttctggatag ggaaatcatt gtcatggcta
     4921 ccgacatcaa tgcatatgct ccctatatcc gcgctgttat ggataatgca ccttatagat
     4981 tcaaatacaa tattgccgat gaagctttgg ctaatgtcaa cagtcccgtt gcagccctga
     5041 tttcgctgct ggaatttgac ggcaagctgt ttggtgcaga ggaagttctt gggctattga
     5101 cacataagta tatccgggaa aaattcagaa taaatgattt ggaattgata cgaaatacca
     5161 tcgtgaaggc taatgcgcgc ttcggtattg aaaacgacta ctcaactcca gtgaatgata
     5221 cctttatcgt ttcctggcgt tacgcattgg agaagatttt ctatggcatt tgtttgcccg
     5281 gagagattag aatagataat gaacatccat tctataccgt cgaaacaacg gatactgtgg
     5341 ccggaattca ggaaatatcg gcattcctgg cgtttttcga ggctttaaaa actcatgtac
     5401 atgagagaaa aaatccaaag ccattgaggg agtggcgcaa attcatcgag caggtattag
     5461 aggatatgtt tgcaattcct gacgatgaaa agaacgaaga ttatgtcaaa aagattgaaa
     5521 aactgatcag atttgaaaat ctggatgatg ggttgaaaga tgaagagatt ccatatgatg
     5581 tattcagtaa gcgtttcttg accggattga aagtagaaac aacatcgact cgttttatga
     5641 gccgtggaat cactttttgt tcccctttac ctttcagaag tcttccattc aagctggttt
     5701 gtatgttggg ccttaatcat ggagaatatc cccgcttgga gcggcgcagt gactttgacc
     5761 tgattgcatc caacaggaag cttggcgaca ggaatcttaa ggaaaacgat aagcatcttt
     5821 tccttgaatc catactttct gcggagaaag ctttatattt gagttatatc ggtcggtcga
     5881 taaaagataa tgagattctg cctccctcta tccttatcga tgaattgctg gattatctgc
     5941 agatggggaa tgtggaggtt gatgttagaa aaaaattggt ttctgagcac ccattttaca
     6001 gttttagtac aaaatataat aaggattcgg gcaatttgct tccgaattat ttcatccaac
     6061 ttgccaagga gtggccgatt ccatataatg acaacagaga agtgccggag attaaggaat
     6121 atgatatcaa tacagtttgg aaattttttg agaagaatat tcaatattat tacaacaatg
     6181 tgctgggggt gtattttgaa agggaagacg gtgacattaa agataccgag aattttgaat
     6241 tggatagtct ggaagaatgg acggtgaagg aatcactcct tgattcaaga ttgaaaggag
     6301 tagagcaggc attagaggaa aaactctatt tatccggaca attgccattg aaaaacgtag
     6361 gattaggact ggtagatgag atagatgaag tgatcagtcc tttggtcgac gatgtcaaaa
     6421 atgattttcc tgataatttt gatccgaaaa ttgtaagtat tgaaataact tatgatgatg
     6481 tacggtattt tggtgagata aagaatgtaa ttgataagaa aataataagt tttcaccacg
     6541 ggagttcagg tggagaggca aagcgtcgtg ccaggttggc tttccagtat ctttttgctg
     6601 tagtatgcaa tgtcgacatt acaggtggcg ctctatacac cctcaacggc aagcatacct
     6661 taaagcaact aggtgttgat gaggcgcggg caaggttggg taatatctta acgttttttc
     6721 atgaaaacaa agagaggctg ataggataca gcgctgaaca atattttcaa tcgcttaaag
     6781 aagatgattc atttgataag attaaaaatt tcttctttat cgatagaaat tatcgtgatg
     6841 attattatag acatgtaatg ctaaaccatg atatcaatat ccaccgggtg tgggacatgc
     6901 atgtcatcct gcaagaaata ttattggata atgcctatta aaatggacaa ttcaatggaa
     6961 gccataaatt ttaaggtaaa cgaagtgcca ttatcaggaa ccaatctgat tgaggctagt
     7021 gccggtaccg gtaagacgtt ttctattggg attttggtgc tcagaacgat tgttgagaag
     7081 aatattccga tcgataatca attgatcgtt actttcacca attatgctgt tgctgaactc
     7141 caggacagga tacgaaaatt tctttctcag gctattgaaa tcgcttcggc ttcagaacag
     7201 gagttagtat tgatagctga tagatttgag caacctattg tggaaatatg tgagagattc
     7261 gaagataaga cagagttgat aacaagactc cggcagtctc aacttctttt agatgaagca
     7321 agtattttta caatccatgg tttttgccag cgaatgttga atgaatttgc ttttgaatca
     7381 ggtcagaatt ttcaaactga attacaggcc gatgtttctt acctgattga acgggtaacg
     7441 aatgaattct ggcgaaatga aatcaccact ttggataaag acctctttga atctaaagat
     7501 ttaaagaaag ttcgcgccta tttcaaggaa attttaaagc aaggtcttaa taatacgcag
     7561 tttgacgtaa atgtttccag cccaattaag gataatggga aaatttctga tattattgaa
     7621 tgttacaagg gatatttaaa ggaattggaa acagagtggg agcggcttga gtgcttcata
     7681 gctgaggata atacacggct aaaagtggat gttgaaagca attcaaatac ccggaagaaa
     7741 tattcagact tattgggtca gcccaggttg atgctgcaag tgacctttga ttacttcaaa
     7801 gcgaataaag cagctggata ttttgataaa ttttcatcac cctactggaa tgatgtaaaa
     7861 tcattggtcg aacgagagaa taatctgaac aatg
//
LOCUS       DAOQMC010000105         7808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3756_length_7808_cov_4.697536, whole genome shotgun sequence.
ACCESSION   DAOQMC010000105 DAOQMC010000000
VERSION     DAOQMC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3756_length_7808_cov_4.697536"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1078
                     /locus_tag="PKV31_05490"
     CDS             206..1078
                     /locus_tag="PKV31_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNB92341.1"
                     /translation="MKNIKIFMLFLIASLFLISCTKEEIKNPINNINETEGLLLVKSF
                     ESNDIKAEVFNGSGTLSVGFNKIFIRLTDKQGNFIKQSSISLLPMMTMNMHGNTHQHS
                     CPVSGITKVEGKETLYEGYIVFIMPSGDSENFWELTFTCNLDGQTYKISDKINVLPSE
                     TEFSKKFSSATGSDGKKYFLALVEPAQPKIGVNDIVVALFQRDDSGNFPIVDNYKMMV
                     DPRMPGMGNHSAPGNKDLEQLSDGLYHGKVGFSMSGYWKINLMLENEAGEVIKGEQVT
                     LQNQESSLNFKLEF"
     gene            1151..3178
                     /locus_tag="PKV31_05495"
     CDS             1151..3178
                     /locus_tag="PKV31_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92342.1"
                     /translation="MKRIILFIAILGTAFQVVGQVNQDSLQEAFLDSIQFRELGEVVV
                     ISNATARSMKDSKALGSLDNYLKNSSSVNMVRRGAYAWEPLLNGMATERSVITVDGMR
                     IYHACTDKMDPVTSYVENTNLSKANINDGQSGAEHGGTIAGSIDLVRRKSGFKTFPKF
                     GGSAFAGFESNNMQQIYGATLNYGSPNFFTDVDFTYRDAENYKAGQRSGLSSEVLYSQ
                     FTKYNLSAISGFKFNDRQHVEASVIFDKATNVGYPGLPMDVALAQATIASLQHSYRSP
                     SPHVDFIETKAYYNTITHIMDDSRRPVVPIRMDMPGWSKTAGFYSKLAGNYNRHTFKA
                     TLSGHLNNSLAEMTMHPNNPNEADMYMLTWPDVNTIYAGINIEDNISLNDKLNLSIQG
                     GIGGHYNEIRSETGLNSLHLFYPDLKATKLRSIKNLSGHLQLKNGRFQHKMGIGYGDR
                     APSVSEAYGFYLYNVNDNYDYIGNPNMKNEKSFSLEVSSGYTAEKISVRWKANYFYIK
                     DYIIGKPQDEFFPMYITASGVKVYQQLEYAGILNTAMNVSYNILKDLLFQADASYRYG
                     QGAQETILPSIQPLGYSAKLKYQKNSFFAEAGIEGCSKNRNSPEFGEIQKPAYMVANV
                     ALSNDFSIGNINLVVKLGVENLFDKYYSTFDDWFGIPKMGRNIFTNVIFKF"
     gene            complement(3245..5005)
                     /locus_tag="PKV31_05500"
     CDS             complement(3245..5005)
                     /locus_tag="PKV31_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB92343.1"
                     /translation="MLTAAVASAQKIEVFGLTKDSLIWKKYVSEMIAADNPDTFALFA
                     TCRTIIDTLQAYGYYSAAVDSIKVRDGKTGIYFYQGKKFNVRDLIIMPYMNKQERDTI
                     TTPEKDTIPMRERIRKEQRKMIETAILNGFPFAATHLEPIIIDSQSVDMKLLLSPGDL
                     YTIGGLHVIGDLKISNFYLEQMLGLRVGAPYYPGLPDKAGKKIRELTFAEQYKSPAVV
                     FAEKKAWLALFLKKKNVNRFDLLIGFQPNTSNIPGVSNKFLLTGNATIELLNQFGKGE
                     KLLGEYFQPSPMQQQLRLEGEYPFLLATPLGVKGSFKLFRSDSSFIEKIIEFGGQYYF
                     GYDSKLEAFWKRQQSDINTVDTSKVIATKKLPASLDYRTDYFGLTYNIQHLDFRLNPR
                     KGFSVDVQMGIGQRRIFKNDKILAIKGPDQSGFDYRTLYDTVTLRSTLFRPVIDSRIF
                     FPLFKRMAIMIRANGAMIAGPKLVYNNEKFRIGGNKTFRGFNEQSFYTTSYLIATTEV
                     KMVLDELSALFVFADNGWINSTSTIRLNNYLWNMGVGAGINFGTKVGVFGLSIAVGKN
                     RSTPFNLQAAKIHFGYQSLF"
     gene            5492..6271
                     /gene="paaG"
                     /locus_tag="PKV31_05505"
     CDS             5492..6271
                     /gene="paaG"
                     /locus_tag="PKV31_05505"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HNB92344.1"
                     /translation="MNYQYIEFTKDGNIATIRFNRPEVFNSVNKPMAMEIQEALDRCK
                     DEDVRCILITGTGKAFCAGQDLNEVVDPNGPELRSIVKEHYNPIIRRIREIEKPVICA
                     VNGVAAGAGANIALSGDIVVAARSASFVQAFSKIGLIPDSGGTFFLPRLIGWNRASAL
                     MMTGDKVSADDAILMGMLYKVYEDEVFQDKIKALAAQMASMPTYGLALTKKALNASNV
                     NDLNTQLDLEEQLQTSAGGSYDYNEGVVAFLEKRMPVFKGK"
     gene            6361..7497
                     /locus_tag="PKV31_05510"
     CDS             6361..7497
                     /locus_tag="PKV31_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNB92345.1"
                     /translation="MIQTVAVIGSGAMGTGIGQVAATHQCTVIFCDASAESLEHSRHI
                     LTSSIERMVEKVKLTGEAGRDIIGRCRWTSDMKEIAGADLIIEAIVEKFEAKEALFKA
                     ISEIIPDQTIVATNTSSLSITALAATISHPERFIGLHFFNPAPIMRLVEIVPALQTEK
                     TLVEKMQHLMASWAKTAVVARNTPGFIVNRVARPFYGEALRIFDEGLAGCRAIDTAMK
                     EVGGFRMGPFELMDFIGHDVNYRVTESVYNAMFQDPRYKPSLTQKSLLDAGWLGRKTG
                     RGFYAYPDMEETMTSYSSGLNPTEIFGRIFAMLVNEAVDAVYMNICSEEDVENAMQLG
                     TNYPQGLISWGRTFGMDRIMDIMSGLYERYREDRYRPSPLLTNI"
     gene            7494..>7808
                     /locus_tag="PKV31_05515"
     CDS             7494..>7808
                     /locus_tag="PKV31_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB92346.1"
                     /translation="MIYEILGYIGAFLMGISLGAIGGGGSIFSVPILVYLFGVNPVIA
                     TGYSLFVVGTTSLSGSLSHIRVGNINWRAVFFFGIPSIASVFITRTLIVPAIPDPVFV
                     GTW"
BASE COUNT         2265 a         1660 c         1711 g         2172 t
ORIGIN      
        1 atcttcatca attcagccag cagaaggatt ttgatctgaa tgaaatccca gcgcacaact
       61 tcaggtatct ccaacttaat ttaaaaccaa gtgtaaccga aattgatcat cctccggctt
      121 tagtttaaat aaattttaat gattcacaaa agtcttcaaa tgatgaaaac gaatcacaat
      181 ttattaaact aaaatttcaa ataaaatgaa aaatattaaa atattcatgc tatttctgat
      241 agcatctctt ttcctcatat cttgtactaa ggaagaaatc aaaaatccaa ttaataacat
      301 caatgaaaca gaaggcctgc tattagtcaa atcatttgaa agtaacgata ttaaagcaga
      361 agtctttaac ggctcaggta cactaagcgt aggcttcaat aaaatattca tccgcttgac
      421 cgataaacaa ggcaatttta tcaaacaatc ctctatctcc ctgttgccga tgatgaccat
      481 gaatatgcat ggcaacactc atcagcatag ctgccctgtt tcgggcatta ccaaggtcga
      541 aggcaaagaa accctgtatg aaggatatat agtattcatc atgccgagcg gagacagtga
      601 gaatttctgg gaattgacat ttacctgtaa ccttgatgga cagacgtaca aaatatcgga
      661 taagatcaat gtgttgccat ccgaaactga attttccaag aaattctctt ctgccacagg
      721 aagcgatggt aagaaatatt ttctggccct ggtcgaacct gctcaaccta aaatcggggt
      781 caatgacatc gttgtggctc tttttcaacg ggatgacagt gggaattttc cgatagttga
      841 caattacaag atgatggttg accccagaat gcccggtatg ggcaatcaca gtgctcccgg
      901 aaacaaagac ctggagcaat tgagcgatgg cctgtatcat ggaaaagtag gattctcgat
      961 gtcaggatac tggaaaatca acctgatgct tgaaaatgaa gccggagagg taatcaaagg
     1021 cgagcaggta acattacaga atcaggaaag cagcttgaat ttcaaattgg aattttaatg
     1081 tcagtatttt gaccaaaagc ataaatgcac agttttcagt gcatttatgc tttattataa
     1141 ttttttaatc atgaaacgaa taattttatt catcgcaata ttaggtactg cctttcaagt
     1201 tgttggtcaa gtaaatcagg atagtcttca ggaagcattt ttagatagca tccagttcag
     1261 ggagttgggt gaagtagttg taatctccaa tgctactgca cggtccatga aagactccaa
     1321 ggcccttggc tcattggata attaccttaa gaactccagt tctgtcaata tggtcagaag
     1381 gggtgcgtac gcctgggaac cattgctcaa cggcatggcg actgagcgaa gcgtcatcac
     1441 cgtagacggt atgcgcatct atcatgcctg taccgacaag atggaccctg tgacatccta
     1501 tgtggagaac accaatctct ccaaagccaa tatcaatgac ggacaatcag gtgcagaaca
     1561 cggagggacc attgcaggca gcatcgactt ggtaaggcgt aaatccggct tcaagacttt
     1621 cccaaagttc ggaggttcgg cttttgccgg ctttgaaagt aacaatatgc aacagatata
     1681 cggagcgacc ttgaattatg ggagtccgaa tttttttacc gatgtagact ttacctatcg
     1741 ggatgcagaa aattacaagg cagggcagag aagtggcctg agtagcgagg tgttgtattc
     1801 tcaatttacc aaatataatc tttcggcaat atcaggcttt aagtttaatg acagacagca
     1861 tgtggaggcc tcagtcatat tcgacaaagc aaccaatgtt ggatatcccg gcttacctat
     1921 ggatgtcgct ctggctcagg caaccattgc ttcactccag cacagctatc gaagcccttc
     1981 gccacatgtt gactttatag agaccaaggc ctattacaat accattaccc atatcatgga
     2041 tgatagtcgc cgtccggttg tacctattcg gatggatatg ccgggttgga gtaagaccgc
     2101 aggcttttat tccaaattgg caggcaatta caatcgtcat accttcaagg ctacactgtc
     2161 cggtcacctc aataattcat tggctgaaat gaccatgcat cccaacaatc caaatgaggc
     2221 ggatatgtat atgctgacct ggcctgatgt aaataccatc tatgccggaa tcaacataga
     2281 agacaatatt tctctcaatg acaaattaaa cctcagcatt cagggtggca tcggaggtca
     2341 ctacaatgaa atccgaagtg agacgggatt aaacagcctc catttgtttt acccggactt
     2401 aaaggcaacc aaattgcgat ccattaaaaa cctttccggc catcttcaac taaagaacgg
     2461 acgattccag cacaagatgg gtattgggta tggcgaccgc gccccaagtg tttccgaggc
     2521 ttatggattc tatctgtata atgtaaacga caactatgac tacataggca atccgaacat
     2581 gaagaatgaa aaatccttca gcctggaagt ctcctctggt tatactgctg aaaaaatctc
     2641 tgtccgttgg aaagcaaatt atttttatat aaaggattat atcattggca agccccagga
     2701 tgaatttttc ccgatgtata tcaccgccag tggagtaaag gtatatcaac aattagaata
     2761 tgcagggata ttgaatacag cgatgaacgt aagttataat atcctgaagg acctgttgtt
     2821 ccaggctgat gcatcatacc gatacggcca gggtgcacag gagaccatac ttccttcgat
     2881 ccagccattg ggatattccg ctaagttgaa gtatcagaaa aacagtttct ttgcagaggc
     2941 aggcattgaa ggctgcagca agaacaggaa cagtccggaa ttcggagaga ttcaaaaacc
     3001 ggcgtatatg gtggccaacg tcgccttgtc caatgatttt tctattggca acatcaatct
     3061 tgttgtcaag ctgggggtcg aaaatctgtt tgacaaatat tattcgactt tcgatgattg
     3121 gtttggcatc ccaaagatgg ggcgcaatat ttttactaat gtgattttca aattctgaca
     3181 ataaatcatc gaatccatat tttccttgtc gtggattgaa caaaacttta atttgctttg
     3241 gatatcagaa caaactctgg tatccaaagt ggattttggc agcttgtagg ttgaaaggag
     3301 ttgatcggtt ctttccaaca gcaatactca atccgaaaac ccctactttg gttccgaagt
     3361 tgattcctgc acctacgccc atattccaca gataattatt gagccgaatg gtactggtcg
     3421 agttaatcca tccgttatct gcaaagacga acagtgcact caattcatcc agtaccatct
     3481 tgacttctgt agtggcaata aggtaagatg tcgtatagaa agattgttca ttgaatcccc
     3541 ggaatgtctt gttgcctccg atcctgaatt tttcattatt atataccagc ttgggacccg
     3601 ctatcatagc tccattggct cgaatcatga tagccattcg tttgaacaat ggaaagaaga
     3661 tcctgctgtc aatgacaggt ctgaaaagtg tggatcgcag tgttactgta tcataaagtg
     3721 ttctgtaatc aaaacctgat tggtcagggc ctttgatggc caatatctta tcattcttaa
     3781 agattcgccg ttgaccaata cccatctgaa catcaactga gaagcccttg cgtgggttga
     3841 gccgaaaatc gagatgctga atattgtacg tcaatccgaa ataatccgtc ctgtaatcaa
     3901 gactggcagg caattttttt gtagcgatga ctttcgatgt atccaccgtg ttgatgtcag
     3961 attgctgacg cttccagaaa gcttccagct tgctgtcata gccaaaataa tattgtccgc
     4021 caaattcaat aatcttctcg atgaaggaag agtcagatcg gaacaatttg aaacttccct
     4081 ttacgcccaa tggtgtagcc aaaagaaatg ggtattcgcc ttccagcctc aactgttgct
     4141 gcattggtga aggctggaaa tattcaccca ataatttttc tcccttgccg aattgattga
     4201 gcaactcgat ggttgcattt cctgtgagca gaaatttgtt gcttactccg ggaatattgc
     4261 tggtattagg ctggaaacca atcaacaagt cgaatcggtt gacatttttt ttcttcagga
     4321 ataaagcaag ccaagccttt ttctctgcaa atacaacagc ggggcttttg tactgctcgg
     4381 caaaggtcag ttcccgtatc ttcttaccgg ctttgtcagg caatccggga tagtaaggtg
     4441 cgcctaccct gagtcccagc atttgctcaa ggtaaaagtt cgatattttc aaatccccta
     4501 taacatgtag cccaccaatg gtataaagat ctcccggtga caacaacaac ttcatatcga
     4561 ccgattggct gtcaatgata atgggctcta agtgagtagc agcgaatgga aacccgttga
     4621 ggatagccgt ttcgatcatt tttctttgtt ccttccttat ccgctccctc ataggtatgg
     4681 tgtccttctc aggcgttgtt atggtgtccc tctcctgctt attcatgtat ggcatgatga
     4741 tcaggtcccg aacattgaat ttcttgccct gatagaaata gataccggtc tttccatccc
     4801 gtacctttat tgaatcgaca gctgcagaat aataaccata ggcctgcagg gtgtcgatga
     4861 tagtccggca tgtagcaaat aacgcaaagg tatccggatt gtcagctgca atcatctctg
     4921 acacatattt tttccagatc aagctatcct ttgtcaatcc gaacacctcg attttctgtg
     4981 cggaagcaac tgcggccgtc agcaataaaa cgcacattaa tatagcctga gcggtatttc
     5041 ggaaattgtg gtacattttt gtatcagtga tcggtcggat attatgtctg cttagagtca
     5101 ccatttacat taacgaagat acttatattt agtctttatg ggatgatata aatatttgcc
     5161 aaggtcatgg caaacggtca aaatcgaagt aggtaggaaa gtaataagtc aaagccactg
     5221 atggattgaa gaatgggaaa atgaaaaaaa ttaatacctg tatcttgaaa caaaaaataa
     5281 attactccct tgaagatgcc cgactacact tagttttcag ctattaaaca agtacacact
     5341 tcgatttttt ctgtccccac cgtattgtaa cctccaggta tccaatcact gtattttcca
     5401 tatttctgaa ggtcaaaagc agagtcattc cttcccggct gaaatgaata gttgcccaat
     5461 tttttgtagg tttgccacaa actattcctt catgaattat caatatattg aatttactaa
     5521 ggatggaaat atagccacca tccgtttcaa caggccggag gttttcaatt cggttaataa
     5581 accaatggca atggagatac aggaggcgct ggaccgatgc aaggatgaag atgtgcggtg
     5641 tatcctcatt acgggtacag gtaaggcgtt ctgtgcagga caggatttga atgaagttgt
     5701 ggatcccaat ggtccggaac ttcggagtat tgtaaaggag cattacaacc ccatcatcag
     5761 gcgaatcagg gagatcgaaa agcccgttat ttgtgcggta aacggagtcg ctgccggtgc
     5821 cggagcgaat atagccttga gtggagatat cgtagtggct gcccgatcag cctcatttgt
     5881 gcaagcattc tcgaagatag gcctgatacc ggatagtggc ggaacatttt ttctgccccg
     5941 cctcatcgga tggaatagag catctgcact gatgatgacc ggcgacaagg tttctgccga
     6001 tgatgctatc ctcatgggta tgttgtacaa ggtatatgaa gatgaagtat ttcaggataa
     6061 gataaaagca ttggctgcac aaatggcttc catgccaaca tatggcctgg ctcttaccaa
     6121 aaaggcgctc aatgcttcca atgtcaatga cctcaatact caattggatc tggaggagca
     6181 actacagaca agtgctggag gttcttatga ctataatgaa ggagtagtgg catttctcga
     6241 aaaaagaatg cctgtattta aaggaaaata aactttcaat ccatcgcttt gtttaagtca
     6301 ggatttgagc attggcatgc taaaaaccaa tcttttcaat ccctttatca ttaaaacaca
     6361 atgatccaaa ccgttgcagt tataggttca ggcgccatgg gaacaggcat aggtcaggtt
     6421 gctgcgacgc atcaatgtac ggtgattttt tgcgacgcat cagctgaatc acttgagcat
     6481 agtcgccata tcctgacatc atccattgaa agaatggttg aaaaagtaaa gctcactggt
     6541 gaagccggtc gtgacatcat cggaagatgc cgctggacaa gtgatatgaa agagatagca
     6601 ggtgccgacc tgataattga agcgattgtt gaaaaattcg aagcaaagga agcattgttc
     6661 aaggccattt ctgaaatcat tcccgaccaa accattgtgg cgaccaacac ttcgtccctg
     6721 agcatcactg ccctggctgc tacgataagc catcctgagc gttttatcgg acttcatttc
     6781 ttcaatccgg cccctatcat gaggttggtc gagattgtgc ctgcattaca gaccgaaaag
     6841 accttggttg aaaaaatgca gcacctcatg gcttcttggg ccaagacagc tgttgttgcc
     6901 cgcaatacac cgggcttcat tgtcaatagg gtggccaggc cgttctatgg tgaggcttta
     6961 cgcattttcg acgaagggct tgccggttgc agagctatcg ataccgccat gaaggaggtc
     7021 ggcggcttcc gtatgggacc ttttgaattg atggacttca tagggcacga tgtcaactat
     7081 cgggtaacgg aatccgtata taatgcgatg tttcaggatc caaggtacaa accgtctctg
     7141 acgcaaaaat cccttttgga tgcaggctgg ttgggtagaa agaccggaag aggcttttat
     7201 gcctatcctg acatggaaga aaccatgacc tcatattcat ccgggttgaa tcccactgaa
     7261 atatttggcc gtatttttgc catgctcgtc aacgaagccg tcgatgcagt atatatgaat
     7321 atctgctcgg aggaggatgt agaaaatgca atgcaacttg gcaccaatta tccacaggga
     7381 ttgatcagtt ggggcaggac attcgggatg gaccgcatca tggatatcat gtccggcctc
     7441 tatgagaggt acagggaaga tcgttacaga cccagccccc ttttgaccaa tatatgatat
     7501 acgaaatatt aggatatata ggagccttcc tcatgggaat atcactagga gccattggcg
     7561 gtggcggttc gatattttct gtgcccatcc tggtatattt atttggtgtc aacccggtta
     7621 ttgccacagg ctattccttg tttgttgtag ggacaacaag tttatccggc tcattgagcc
     7681 atatacgtgt cggcaatatc aactggcggg cagtattttt tttcggtatt ccatcgattg
     7741 catctgtctt tattacccgt acacttattg tacccgccat tcccgatccg gtgtttgtcg
     7801 gaacctgg
//
LOCUS       DAOQMC010000106         7767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3784_length_7767_cov_4.695799, whole genome shotgun sequence.
ACCESSION   DAOQMC010000106 DAOQMC010000000
VERSION     DAOQMC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3784_length_7767_cov_4.695799"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKV31_05520"
     CDS             <1..705
                     /locus_tag="PKV31_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB92347.1"
                     /translation="EKQIQGLNRMRTQRDPIRFREAMDKLKVAAEGSGNLLEACVDAA
                     RARASLGEISKVMEECFGRHQAQSKMISGVYLRNMGDNRMFKEAKELSDEFAKKEGRR
                     PRILVAKLGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPEEVAMQAAENDVHIVGIS
                     SLAAGHKTLVPATIAELKKIGRGDILVVAGGVIPPDDYRFLYDAGVSAVFGPGTPIPQ
                     AAADLLRILINALHPG"
     gene            706..1500
                     /locus_tag="PKV31_05525"
     CDS             706..1500
                     /locus_tag="PKV31_05525"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNB92348.1"
                     /translation="MGKIVFASDFHLGQKGTTSSEEREKALVCWIDEVAMQAEEVFFL
                     GDTFDFWYEYKSVIPKGFTRFLGAMARLADAGIPIHLFTGNHDMWIFDYFTKELGIPL
                     YRQPTEFVRQGRRMLIGHGDGLGPGDRRYKFLKKIFNNRACIWLFHWLHPSIGMWIAN
                     KWSSSSRESNNDYVFTGPEKEYLLRYCEGELKSGANFDYMIFGHRHLPIDYLLSNEKT
                     RYINTGDWLRFKSYGVMEDGKIELKFYNEPGQSVISNDPESWKSFS"
     gene            1659..1829
                     /locus_tag="PKV31_05530"
     CDS             1659..1829
                     /locus_tag="PKV31_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92349.1"
                     /translation="MKFKFGGSKVDHIDKFVDTLWWIVSFKPERVRAYIAICFMAVQG
                     YYNTGPIYSIIQ"
     gene            complement(2154..4316)
                     /gene="metG"
                     /locus_tag="PKV31_05535"
     CDS             complement(2154..4316)
                     /gene="metG"
                     /locus_tag="PKV31_05535"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNB92350.1"
                     /translation="MAVRLPKRYLVTSALPYANGPLHIGHLAGAYLSADIFVRFLRLM
                     KKDVLYVCGSDEHGAAITIRAQKDGVTPHEIVDKYHAMFEETFRRMGISFDIYHRTSS
                     PLHHETSQDFFRTLVKKDQFIIQESMQFYDEKARQFLADRYIKGTCPNCKFEGAYGDQ
                     CENCGTSLNPTDLINPVSMLSGETPVLKATTHWYLPLENHSKWLSTWLEKGELDGKIV
                     HDPSEWKNHVLGQCKSWIEGGLQPRAMTRDLEWGVDVPQELPGAKGKKLYVWLDAPIG
                     YISATKQLASDRKLDWKKYWQDKDSALVHFIGKDNIVFHSIIFPALLKAHGDYIMPDN
                     VPANAFLNLEGQKISTSRNWAVWVHEYLDDMPGKEDVLRYVMIRNMPEQRDSEFTWKN
                     YQELNDNELVGNLANFVNRVMVLTHKFYGGKVPKFEEEQDFIGSHGPLEDSFVEAELM
                     DVFDMLFEMREKIYLYDFRGALQQLMNISTMGNQILQFNEPWKIHEEDPNKVKAIMNC
                     CIQIVVALSVAVRPFLPFTSDKMRTMLNLPPIEEKGELLKVLDRLAEGEFLVKPGHVI
                     GKPEHLFTRIPDEAIQAQIDKLKASHNTPETKTMENKATSASENGQAEIQFDDFAKVS
                     IVTGKILEASKVEKADKLLQLLIDIGTEKRTIVSGIAMHFKPEDLVGKDVMVVANLAP
                     RKLRGIESKGMLLTAESADGKLGIVSPPEGWPVGSHVG"
     gene            4760..5509
                     /locus_tag="PKV31_05540"
     CDS             4760..5509
                     /locus_tag="PKV31_05540"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNB92351.1"
                     /translation="MRKKMLKRQDTHIVAVHQPNFFPWFGYFVKIALSDTFILLDDVQ
                     IPWRGYTRRTKIRRPGNDPLLEWCPVIIPSGYTYAGINKIPLDDRRQWQGELSGRLES
                     RYRSGAGFKELFPRVQAWLNNGATNLATYNTAIIRNIMDLMDIRKELNVSSDYPVSSS
                     GSARIVELMQYFGGNVYLSGRGGNKYHRENDFIEAGIKIIEVPNLLESPLLMEVEGIN
                     PEASILEWLFMLGIDRTREFFYKYLDTIVIG"
     gene            5506..7434
                     /locus_tag="PKV31_05545"
     CDS             5506..7434
                     /locus_tag="PKV31_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB92352.1"
                     /translation="MKRLMIFLVIGGLIQSCAIAQPLVTLKDASDKTKKVFDEGRKAA
                     MAQSFGTAKAKYREALQLTPGFQDAMMELAGIYFSQKNMDSSIFYLNQILTVNPKPSA
                     RVYFTLGTIYYDRYEYREAIPLFEKYLQSDVKNPKSVEDAKIKLANSQFAIEALKNPR
                     SFEPKPVPGEINTELPEYLPSESADGTVLVFSRMIDGQEDLYFSSYDGNSWSRAVPLD
                     AINTEHYNEAGQCISADGHTIVYTGCNMPGGLGSCDLYISYLENGKWTRPKNMGAPMN
                     TPGWESQPSLSADGSTLYFASDRLGGYGLKDIWFSERSPDGVWSTPKNIGAPVNTLKN
                     DGSPFIHPDNETLYFMSDGLPGMGGTDIYLSRRQEDGSWGTPVNLGYPINTSGNEGAL
                     VVSPAGDIAYYTAIKDGGKRSKEGFDDTDIYYFQLDSAIRPKPVSFFKARMLDAMAGK
                     PVRGLVQVTDIRTDSLYYKGLAADDGTVLVCLPKGGLYGIQIIARGYLFVSESVEIPD
                     GGDADHPFIAEYLLHRIEESKGKAMVLKNIYFTSGSAELSPVSDAELKVLAAMFFANP
                     DIRVRINGHTDDVGSDEDNIQLSVMRANSVMERLIELGVSAKSLSAKGFGESVPIGDN
                     TTEAGRKSNRRIEFEILP"
BASE COUNT         2147 a         1550 c         1889 g         2181 t
ORIGIN      
        1 gagaaacaaa tccagggttt gaaccggatg aggacacaaa gagatcctat cagattccgg
       61 gaagccatgg ataagttgaa agttgccgct gagggaagtg gtaatctgct agaggcttgt
      121 gtggatgcag caagagccag agccagcctt ggggagatca gcaaggtgat ggaggagtgt
      181 tttggccggc atcaggctca atccaaaatg atttcaggcg tatatttgcg caatatggga
      241 gataacagga tgtttaaaga agcaaaggag ctgtcggatg agtttgccaa aaaagagggc
      301 aggcgcccga gaattcttgt cgctaagttg ggtcaggacg gtcatgaccg tggtgccaaa
      361 gtgatagcaa caggctttgc cgatcttggt tttgatgtgg acatcggtcc gttgttccag
      421 actcctgagg aagtagccat gcaggctgcg gaaaatgatg tacatatcgt gggaataagc
      481 tcacttgctg cagggcataa aaccctggtg ccagccacaa ttgccgagtt gaaaaagatt
      541 gggcgtggtg atatcctggt tgttgccggt ggtgtcattc cgcctgatga ttacaggttt
      601 ttatatgatg ccggtgtgag tgcggtattc ggtccgggca ctcccattcc gcaggcggcg
      661 gctgacctgt tgaggattct catcaatgca cttcatcctg gataaatggg gaaaatagtt
      721 tttgcttccg actttcacct tggccaaaag ggcacgactt cgagtgaaga gcgtgagaag
      781 gcacttgttt gctggatcga tgaggtagcc atgcaggcag aggaagtgtt ttttttgggt
      841 gatacatttg atttttggta tgaatacaag tccgtgatac ccaagggatt tacacgtttt
      901 cttggtgcga tggcgcgcct ggcagatgcc ggcataccga tccatttgtt taccggcaat
      961 catgatatgt ggatattcga ctattttacc aaagaactgg ggattccact gtatcggcag
     1021 ccgacagaat tcgtgcggca agggcggaga atgctgattg gccatggcga tggattaggg
     1081 cccggtgatc gacgatacaa atttttgaag aaaatattta acaatcgggc ctgtatctgg
     1141 ctttttcatt ggctgcatcc ttccatcggt atgtggatag ccaataaatg gagtagttcc
     1201 agccgggaga gtaacaatga ctatgttttt accggccctg aaaaagaata cctgttgcgt
     1261 tattgcgaag gcgagttgaa gtcgggagcg aattttgact atatgatttt cgggcaccgc
     1321 cacctgccca ttgattacct cctgagtaat gagaaaacac gttatatcaa tacaggggac
     1381 tggcttaggt ttaagtcata tggtgtcatg gaggatggaa aaatcgaatt aaaattttat
     1441 aacgagcccg gtcaatcagt aatttccaat gatcctgaga gctggaaatc attttcatga
     1501 gatagtagga ataaacagag gctgataggg aagaaagaaa tggaagtata gcaaagtaaa
     1561 aatcagaggc ctgaaggatg gtgtctgaag gataattact tgaatacatg gatgctaaat
     1621 ggttataatt caaggttcta tatggaatag cgtgggtaat gaaatttaag tttggcggaa
     1681 gtaaagtaga tcatattgat aaatttgttg acacactttg gtggatagta tccttcaagc
     1741 ccgaaagagt aagagcatat atagccatct gctttatggc agtgcaagga tattacaata
     1801 caggaccaat ctacagtatc atccaatgag catagaagcc attagaacag ccttggtaca
     1861 tacccaatta tcgatactaa gagataaaac gactaagaaa ttatacggac taccctccaa
     1921 actcatgcta gatgcaaaga agctgtacca gttagtagga ctgaaactat ctgatcagcc
     1981 tttcccacta taaacaccca caaatgcttg attgtcaatt gtttaacaat tagacgtagt
     2041 gtctaattga aaatacaact atctgaatat caacatttta gataataaaa gtgcggaaat
     2101 caggagaatt ataagaagac tacattgcct gcccgggtgt tgttctccca ttattacccc
     2161 acatggctgc ccaccggcca tccctcagga ggggatacga tgccgagttt gccatccgct
     2221 gattctgcgg tcaacaacat acctttggac tcaatacctc ttaatttgcg cggcgcaaga
     2281 ttggcgacaa ccatgacatc cttacctacc agatcttccg gcttgaaatg cattgctatt
     2341 ccggaaacta tggtacgttt ttcggtacct atatctataa gaagttgcag taatttgtcg
     2401 gccttttcca cttttgatgc ttcaaggatc tttcctgtga cgatggatac cttagcaaaa
     2461 tcgtcaaatt gaatctccgc ctggccattt tctgaagcgg atgtcgcttt attttccatg
     2521 gtctttgttt caggtgtgtt gtgacttgct tttaacttat caatctgcgc ctggatggct
     2581 tcatccggaa ttctggtaaa taaatgctca ggcttgccga tgacatgccc cggcttgact
     2641 aaaaattctc cttctgcaag ccgatccaag accttgagta gttctccctt ctcttcgatg
     2701 ggtgggagat tgagcatagt gcgcatcttg tcactggtaa atggcaggaa tggtctgact
     2761 gcaacactca aagccactac aatctggatg cagcaattca tgatcgcctt gaccttattc
     2821 ggatcttctt catggatttt ccatggctca ttgaactgga ggatctggtt acccatggtc
     2881 gaaatattca tcaattgctg taaagcaccg cggaaatcat acagatatat cttttcgcgc
     2941 atctcaaaca acatgtcaaa aacatccatt agctcagcct ccacaaagct gtcttccagt
     3001 ggcccatgtg aaccgatgaa atcctgttct tcctcaaatt ttggcacttt gccgccgtaa
     3061 aatttgtgag taagcaccat gaccctgttg acgaaattgg ccagatttcc tacgagctca
     3121 ttgtcattga gctcctgata gtttttccat gtaaattcac tgtcgcgttg ttccggcatg
     3181 ttgcggatca tcacatagcg gagtacatct tcctttcccg gcatatcgtc cagatattca
     3241 tgcacccata ctgcccaatt gcgggaggtg cttattttct ggccttccag gttcaaaaat
     3301 gcattggcag gtacattgtc cggcatgata taatcaccgt gagctttgag cagtgccgga
     3361 aagatgatgg agtggaacac gatattatct ttgccgataa aatgcactaa ggcagaatcc
     3421 ttatcttgcc agtatttttt ccagtctagt ttgcggtcgg aagccagttg ttttgttgca
     3481 gagatatagc cgattggagc atccagccaa acatacagct ttttaccttt ggcgccgggt
     3541 agttcctgtg gcacatccac accccattcc agatctcgtg tcattgcccg gggctggagg
     3601 ccaccttcga tccaggattt gcactgaccg agaacgtgat tcttccactc cgaaggatca
     3661 tggacaattt taccgtctaa ttcacctttt tccaaccagg tactcagcca cttactgtga
     3721 ttttcaagcg gcagatacca gtgtgttgtt gccttcagca caggtgtctc ccctgatagc
     3781 atggacaccg gattgatgag atctgttgga ttgaggcttg taccgcagtt ttcacactga
     3841 tcaccataag caccttcaaa cttacagtta gggcaggtgc ccttgatata ccgatcagca
     3901 aggaattgcc tggctttctc gtcgtagaat tgcatggatt cctggatgat gaactgatct
     3961 ttcttgacca atgtcctgaa aaaatcctgt gaagtttcat ggtggagtgg tgatgaagtc
     4021 ctgtggtata tgtcgaagga gatgcccata cgacggaagg tttcctcaaa catcgcatga
     4081 tacttatcca ctatttcatg cggggtaaca ccatccttct gcgctcttat ggtgatagcc
     4141 gctccgtgtt cgtcactgcc gcaaacatac aggacatctt ttttcatcaa tctaaggaag
     4201 cggacaaaga tatcggcact caggtaagcg ccggcgaggt ggccgatgtg aaggggacca
     4261 ttggcataag gaagggcaga agtaacaagg tatcttttcg gtaaacgaac agccataaaa
     4321 aaccggtttc ttttaaacag ccacaaagat actttattga aatattttcc taaagggttt
     4381 tttgaatatt ttagaaaaat ttgattttaa aacaataaga aagtcagact tttaaatata
     4441 aggattagca acgaagctga attttgaaat tttttttaat gatgttataa gttaatataa
     4501 tatgtaaaaa attgcctgac agagtggatg gttgaaaagt gttttttaac taaaggaatg
     4561 caaatgataa tacttttgtc tggagtgtgt ctatgctcat accggcgaag caagccgggc
     4621 gggaaatgat tgtttcttca ctttttggta ttttgattga actaaatatt tgatttgaag
     4681 tgaatagtta tttcattttt attaacccgt aattatgctt tatgtcaggg atttcaatta
     4741 tcgtacaatt gcactaactt tgcggaagaa gatgttgaaa agacaggata cacatatcgt
     4801 tgcggttcat cagcctaatt tctttccatg gtttggttat tttgtaaaaa tagctttgtc
     4861 ggatactttc atcctgcttg acgatgtgca gattccatgg agagggtata cccgaaggac
     4921 gaagatcaga agacccggaa atgatccttt gctggagtgg tgtccggtaa taattccatc
     4981 cggatatacc tatgcaggta tcaacaagat accgttggat gatcggagac aatggcaagg
     5041 tgaactgtcc gggaggctgg agagtagata tcggtccgga gcgggtttta aggaattgtt
     5101 tcctcgtgtg caggcttggc tgaataacgg ggctaccaat ctggcaactt acaacacagc
     5161 tatcattcgc aacattatgg acttgatgga catcagaaaa gaattgaatg tcagcagtga
     5221 ctaccctgtt tcatcatcgg gttcggcgcg aatcgttgaa ctgatgcagt attttggagg
     5281 aaatgtgtat ttaagcgggc gcggtggcaa taaataccat cgggaaaacg attttattga
     5341 agcaggcata aagatcattg aagtgccgaa tcttcttgag tcgcctttat tgatggaagt
     5401 tgaagggatc aatccggagg cttccatact tgaatggctt ttcatgttgg gaatagacag
     5461 gacgagggaa tttttttaca aatatcttga tacaattgta atagggtgaa gcgattgatg
     5521 atatttttgg tgataggggg attgatccag tcgtgtgcaa tagcgcagcc gttggttact
     5581 ctcaaagacg ccagtgataa aactaaaaag gttttcgatg aaggccgcaa agcagccatg
     5641 gcacagtcct ttggaacagc aaaagccaaa tatagggaag ccttgcagtt gactcccggt
     5701 tttcaggatg ccatgatgga attggcgggc atttattttt cccagaaaaa catggattcg
     5761 agcatatttt acctcaatca gatattaaca gtcaatccta aaccttccgc ccgggtatat
     5821 tttacattgg ggaccattta ttacgaccgc tatgaatatc gggaggccat tcctttattc
     5881 gaaaagtacc ttcaatcgga tgtcaaaaac cccaagtccg ttgaagacgc aaagataaag
     5941 cttgccaata gtcagtttgc aatagaggcc ttgaagaatc ctcggtcttt tgaacccaaa
     6001 cccgtgccgg gcgagatcaa taccgaattg cccgagtatc tgccctctga aagtgctgat
     6061 ggtaccgtat tggtcttctc cagaatgatt gatggtcagg aggatttgta tttttcgagc
     6121 tatgatggca atagttggtc aagagctgtt cctttggatg ctatcaacac tgagcattat
     6181 aatgaagccg gtcaatgcat tagtgctgat gggcacacta ttgtgtatac agggtgcaac
     6241 atgcccggag ggttgggaag ctgtgacttg tatatttctt atttggaaaa tggaaaatgg
     6301 acaaggccaa aaaacatggg agctccgatg aatactcccg gatgggaaag ccaacccagc
     6361 cttagtgcag atgggtccac cctgtatttt gcgagcgacc gcttaggtgg atacggattg
     6421 aaagatattt ggttttcgga aagaagcccg gatggtgtct ggtctacacc gaaaaatatt
     6481 ggcgctcctg tcaatacgct gaaaaacgat ggcagtcctt tcattcatcc tgataatgag
     6541 accctatact tcatgtccga cggtttgccg ggcatgggcg gtaccgatat ttatctttca
     6601 aggaggcagg aagatggctc ctggggtact cctgtcaatc tcggatatcc catcaataca
     6661 tcaggtaatg aaggcgcact ggtagtcagt cctgccggtg atatcgctta ttatacggca
     6721 ataaaggatg gcgggaagcg atcaaaagaa ggatttgatg atacggatat ctattatttt
     6781 cagttggatt cagcgatcag accgaagccg gtatcttttt tcaaggctcg catgctggat
     6841 gccatggccg gcaaacctgt caggggcctg gttcaggtga ctgacattag gacagattct
     6901 ctgtattaca aggggttggc tgctgatgac gggactgtgc tggtctgtct acccaaaggc
     6961 ggcctgtatg gtattcagat catagccagg ggatacctat ttgtatcaga atcggttgaa
     7021 ataccggatg gaggggatgc tgatcatcct ttcatcgcag agtatttgtt gcataggatt
     7081 gaagaatcaa agggcaaggc gatggtcctg aaaaatattt acttcacaag tggaagtgcg
     7141 gagctttctc ctgtatcgga tgctgaattg aaggttctgg cagctatgtt tttcgccaat
     7201 cctgatatcc gggtacgaat caatggccat accgatgatg ttggaagtga tgaggataat
     7261 atccaactgt cggttatgag ggcaaattca gtcatggaac gtttgattga attgggtgtc
     7321 tctgccaaat cattgtctgc caaaggcttc ggggagtctg tgcccatcgg tgataatacg
     7381 accgaagccg gaagaaaatc caaccggcgc atagaatttg aaatcctgcc ataaaggttg
     7441 ctgttttgaa agagacaatg ggccggatgg ttgccataga acaatattac aacggacaat
     7501 tcttttgata taaatattgg ataaattttc agatcaacaa attaaagccc ggtgcattgc
     7561 ttgacacgct ataggccctt gaaaattgca tatattggtg aataatccat gcataattgg
     7621 gcagattttg aattttccga caatatttct tacttttacc cgcatcacaa aacaaaaaac
     7681 gaaacacgct gttatatggc gaatttattc aggaagaaat cgatccagac aatattggcg
     7741 gattctgctg ccgggatgga agatggg
//
LOCUS       DAOQMC010000107         7753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3797_length_7753_cov_5.306183, whole genome shotgun sequence.
ACCESSION   DAOQMC010000107 DAOQMC010000000
VERSION     DAOQMC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3797_length_7753_cov_5.306183"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..618
                     /locus_tag="PKV31_05550"
     CDS             355..618
                     /locus_tag="PKV31_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92353.1"
                     /translation="MKRFFIYFIELTALPVIVIGTGTGLSSYYTATYGVMSRASHGTS
                     VCIQLIGQERQCSITENAQGQVFSVYRKAVNPLIDLSHHSIIT"
     gene            622..1644
                     /locus_tag="PKV31_05555"
     CDS             622..1644
                     /locus_tag="PKV31_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB92354.1"
                     /translation="MADKIRYLSCFVLTYWVLRLIFSSVFIRAGEGLVEGVANRNISG
                     WGDPSLTLQAATPFYGSLLFYDILLIITIISIYLGVWLRSRYLGKVKARLKQNMERQT
                     EVLHHNIIRLEQSERRLLESNRMKDEITAMILHDLRSPIRFVKLMAAYLEKNFDTMPT
                     KQLEQRISALKSSTSALNEFTEQFFTWAASQHQDFKVNKVFFDLKDLFLEIKSLYQDI
                     LVINENEIVIEPCYIIALTDRQILSTIIRNIVDNSNKIMKKGRIHISGNVEYGGVNIF
                     VTDNGPGFEKGKLQKFLGNGQLATPGGHGSIILRNLIKIIEGRLEVYSEEGIGTIFKI
                     TLPSQG"
     gene            complement(1616..2254)
                     /locus_tag="PKV31_05560"
     CDS             complement(1616..2254)
                     /locus_tag="PKV31_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB92355.1"
                     /translation="MLRIIIADDHFAVRVGLEILVRDTVDEDVSIDFASDGLELLSKL
                     KKSYYDVLLTDLNMPGIEGLPMLQKIREISPSLRILVVSVNSEEFYAIKAHELGAYGF
                     VHKASSDEELKEAIAAVCQGEKYVSEHQQRLINDQNPNPFVNLSPRELEVLNLLFKGY
                     GVLEISNELNIGQTTASTFKSRILKKTSTHSVVELIKLAEQYGFIPDSAKLS"
     gene            2704..3426
                     /locus_tag="PKV31_05565"
     CDS             2704..3426
                     /locus_tag="PKV31_05565"
                     /inference="COORDINATES: protein motif:HMM:NF039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HNB92356.1"
                     /translation="MPYPHQVVIVIPVYKPFPDPEEIRALRRAVSVFSRHPIRLLGPD
                     DIDYSVYLDAGIDRCSIIQMPGHHFRGIKAYNRLLCSKGFYAIFDVFEYILIFQTDAW
                     VFRDELSEWCERGYDYIGSPWLWRPERVKNSQILDLWPLMKGRVGNGGVSLRKIKVMK
                     KYALLARIFFALTAKNEDFIWLLVSLLPGVRIKKPNADEALKFCIEMEPEEALNRTGS
                     LPFTVHAYMRYGADYWKAYMPD"
     gene            complement(3880..4110)
                     /locus_tag="PKV31_05570"
     CDS             complement(3880..4110)
                     /locus_tag="PKV31_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92357.1"
                     /translation="MILLYQNMIVKLCYDDYSFLPDRRYNNLSLLSGYLLRLYAKMAS
                     RVDFLGSKRTSGKSCFNVIGELGLSGVSLTKK"
     gene            complement(4197..5111)
                     /locus_tag="PKV31_05575"
     CDS             complement(4197..5111)
                     /locus_tag="PKV31_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNB92358.1"
                     /translation="MKLVTVGTVAFDDIETPRGKAGKVIGGAATYISRSASFFVENQG
                     IVSVIGGDFPQQELDEMTSLGIDISGIEIRKDEKSFFWAGRYHDNMNQRDTLITDLNV
                     LANFQPKLPQSFRTADYLMLGNLTPEVQMNVLQQMTRRPKLVVLDTMNFWMDVAWDSL
                     QKVLRMVDVITINDEEARQLSGEYSLAAAAAKIYRMGPKYLIIKKGEHGVMLFREGRM
                     FSFPALPVSEVIDPTGAGDTFAGGFIGYLARTEDLSFENMKRAIVYASVMASFCVEDF
                     SLAKLKSINHLDIAMRVDIFKDMTHFVM"
     gene            5360..5433
                     /locus_tag="PKV31_05580"
     tRNA            5360..5433
                     /locus_tag="PKV31_05580"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5394..5396,aa:Asn,seq:gtt)
     gene            5486..5785
                     /locus_tag="PKV31_05585"
     CDS             5486..5785
                     /locus_tag="PKV31_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB92359.1"
                     /translation="MHYLYILYSVSSDIYYVGITNNPAKRLSEHNEGVHCTFTSKHRP
                     WEIRAVFEVGESLGKAMVIEKFIKKNRSTRMMQLLCDESYVPDGRLSGMRRVIEY"
     gene            5787..5866
                     /locus_tag="PKV31_05590"
     tRNA            5787..5866
                     /locus_tag="PKV31_05590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5821..5823,aa:Gly,seq:tcc)
     gene            5920..6219
                     /locus_tag="PKV31_05595"
     CDS             5920..6219
                     /locus_tag="PKV31_05595"
                     /inference="COORDINATES: protein motif:HMM:NF022019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB92360.1"
                     /translation="MYYLYILYSVSSDIYYVGITNNPAKRLSEHNEGIHCTFTSKHRP
                     WEIRAVFEVGESLGKAMVIEKFIKKNRSTRMMQLLCDKDFIPDGKLSGMRRVIEY"
     assembly_gap    6326..6425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6637..>7753)
                     /gene="mnmE"
                     /locus_tag="PKV31_05600"
     CDS             complement(6637..>7753)
                     /gene="mnmE"
                     /locus_tag="PKV31_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNB92361.1"
                     /translation="LSRILELCIEAGARAALPGEFTQRAFLNGKMDLAQAEAVADLIA
                     AESAAAHHTALNQLRGGFSRDLDDLRTKLVEFASLIELELDFSEEDVEFADRKQFLVL
                     IDTLKEKTSSLLESFRLGNVIKKGISVAIIGKPNAGKSTLLNALLNEERAIVSDIAGT
                     TRDTIEETLNINGILFRLIDTAGIREHTTDIIESIGMERSRNNATQSDIILYLMDMNS
                     LVSFDDRQNIYDEFQWLKEFSSKTIPVYTKYLLWKENISTDSSIESADSPYILTDAKS
                     GLGIESLKQVIFEKAVGGKINTDQTIITNVRHRDILQKISVSLDKIKTGLNESLSGDL
                     LAIEIHECLRYIGELTGRVDIDRDILGTIFSKFCIGK"
BASE COUNT         2187 a         1442 c         1745 g         2279 t
ORIGIN      
        1 cagatattta atggaaaaag agattaaaaa tagcctttaa gggttttggg gtcaaccgcc
       61 attcgatccg ggaatattta tcaaaactaa caggggacag gggtttggcc aatattccaa
      121 aaaaaaaccg ctcctaaata tggggaaacg gagcggtttt aagtattaga gtatctcaac
      181 cggggttgag caaaattttt cgtgcatgaa tctattgaca atcaatgtct tcgaaaatga
      241 ttattcaaag ataggcattg ttgtaaaaat acatatgtag tattttaact acaaaaagtc
      301 tcagattgct gtcaatatat aacggatagg ttgtaaattg aggcgatttt gtgcatgaaa
      361 cgatttttca tttatttcat tgagctcacg gctctccctg tcattgttat aggtacaggt
      421 acagggctct cttcgtatta tacggcaact tatggagtca tgagtagggc gagccatggc
      481 acatcagtgt gcattcaact tatcggtcag gaaagacaat gctcaattac agaaaatgcg
      541 caaggacagg tattttccgt ttacaggaaa gcggtcaacc ctttgattga ccttagtcat
      601 cattccataa tcacctgaag gatggcagac aagatcagat acctttcatg ttttgtgttg
      661 acctactggg ttcttcggtt aattttttca tctgtattta tcagggcagg ggaaggtttg
      721 gtagagggtg tggccaaccg gaacatttcc ggttgggggg atcccagcct gacattgcaa
      781 gcagcaactc cattctatgg ttctttgctt ttctatgata tattattgat catcactata
      841 atatcaatct atctgggggt atggctacgc tcgaggtacc tcgggaaggt gaaagctcgt
      901 ctgaagcaaa acatggaacg gcagacagag gttttacatc acaatatcat taggcttgaa
      961 caatccgaaa gaaggctcct ggaaagcaac aggatgaagg atgagattac tgccatgatt
     1021 ctgcatgacc tgagatcccc tatccgattt gttaagttga tggcggcgta cctggagaaa
     1081 aattttgaca ctatgcctac taagcagctt gagcaaagga tttccgcctt gaaaagctcc
     1141 acatctgctt tgaatgagtt tacggaacaa ttctttacct gggcggcaag ccagcatcag
     1201 gattttaaag tcaacaaagt attttttgat ttgaaggatt tatttttgga gatcaaatct
     1261 ctctatcagg atattcttgt tatcaacgaa aacgaaatag tcattgagcc atgttatatc
     1321 atagctttga ctgacaggca gattctttct accatcatcc ggaacatcgt ggacaattcc
     1381 aacaagatta tgaagaaagg caggattcac atcagtggta atgttgaata tggcggggtc
     1441 aacatatttg ttaccgataa tgggcctggc tttgaaaagg gaaaacttca aaaatttctg
     1501 ggcaatggcc aactggccac gcccggaggt catggtagta tcattcttcg caacctgatc
     1561 aagatcattg aaggaaggct tgaagtgtac agtgaagaag gtatagggac aattttcaag
     1621 ataactttgc cgagtcaggg ataaaaccgt actgttcagc cagcttgatt agctctacta
     1681 ccgaatgggt tgatgttttt ttgagaatcc gggatttgaa tgtgcttgca gttgtctgac
     1741 ctatattcag ctcattggaa atctcaagca caccataacc cttgaataac agattcagga
     1801 cttccagctc gcgcggtgac agattgacaa atggattggg attctggtca ttaatcagtc
     1861 tttgctgatg ttccgaaaca tatttttcgc cctggcacac tgctgcgatt gcttccttga
     1921 gttcttcatc actggatgcc ttatgtacaa agccatatgc ccccagttcg tgtgccttta
     1981 tggcatagaa ttcctcggag ttgacactta ctaccaggat acgaaggctg ggacttatct
     2041 ccctgatttt ttgtagcatt ggcaaacctt ctattccagg catgttgagg tcggtcaaca
     2101 acacgtcgta ataagatttt tttaacttac tgagaagttc aaggccatct gaagcaaaat
     2161 cgatggacac atcttcatca actgtgtccc tgactaagat ctcaagacca actctcacag
     2221 caaagtgatc atcagctatt ataatcctta gcatagtatt ataattaata gattaataag
     2281 aggttaatat gaaacaaata taataaattt ttggtttgat ccaaattctt tttgtataaa
     2341 gtttgtaatt aaagtcattt gtcttgttca ggcattgatt aaaacaggga tgagaatgtt
     2401 ttatttgttg gtcaatggtt tatcggaggt gaggatttga aatgcctata tcagggacag
     2461 atattccaat ggcataaaga actgatattt actgtgtgga attgttcctc attttcagaa
     2521 taaattatgc ggaatgacat tcagtgacag tgagggagga gcttttggtc tttctggcat
     2581 agtttcagaa ttcggggctt tatggctgtt acaccatcag gtatttgatt ttgggtgaat
     2641 ttgaaattat gttcaatttt acattccaat tcaaaagtta tttcaaaact ttacctttgt
     2701 aggatgccat acccacatca ggttgtaatt gttattcctg tatacaaacc atttcccgat
     2761 cccgaagaaa tccgggcatt gagaagggca gtcagcgtat tcagcaggca tcctatccgg
     2821 ttattaggcc cagatgatat agattacagt gtgtatctgg atgccggaat tgatagatgc
     2881 tctatcattc agatgcccgg ccaccatttt cgggggatta aagcctacaa ccgccttttg
     2941 tgcagcaagg gattctatgc catcttcgac gtctttgagt atatattaat atttcaaacg
     3001 gatgcctggg ttttccggga tgaactctcc gaatggtgcg agagaggcta cgactatatt
     3061 ggatctccgt ggttgtggcg gcccgagcgg gttaagaata gtcaaatatt ggatctgtgg
     3121 ccattgatga agggacgtgt gggtaatggg ggtgtaagcc ttcgtaagat taaggtaatg
     3181 aagaaatatg ctttattggc tcgtattttt tttgccctaa ctgccaaaaa tgaagatttt
     3241 atctggttgc tggtttccct tttgcctggt gtaaggataa aaaaaccgaa tgctgacgag
     3301 gctttaaaat tctgcattga gatggaacct gaggaggctt tgaacaggac aggcagcctg
     3361 ccttttaccg tacatgccta tatgcgttat ggagctgatt actggaaggc ttatatgccg
     3421 gattaagaaa tgcttttcac atttgaatac agctccaata tttgtcgatg taaagaaaag
     3481 tggtatgccg gcaaagtaga ttagagtatc taagcttttg atagctatag agttcaaaag
     3541 ttgggaaaat gagtaatgag ggggagactt tgcattggtg aaatgtctat gattatgttt
     3601 ctatttcccg atttgcacag attattgaca tccacccttt aggccattaa acctaattgc
     3661 ctataaaaaa ttgattctat tgaaaattta gattgatcaa gacgcaatag ctggtttttt
     3721 aatcaaaaaa tattttactt gtaaacaata ataaaatgat aaatactgtt taaagagaga
     3781 aaacaatttt atgtagcatt tttatctatt tgtatctatg agccaaattt gtacaaaaca
     3841 aaattccaat tccacttcaa ataaaatcaa aaatttctcc tacttttttg tcaatgagac
     3901 accggataat cccaattctc ctataacatt gaagcagctt ttgccggatg ttcgctttga
     3961 gcctaaaaaa tccacccttg aagccatttt ggcatacagt cgcaagagat aaccactaag
     4021 cagggaaaga ttgttgtacc tacggtctgg tagaaaggaa taatcgtcat aacacaattt
     4081 tacgatcata ttctgataca aaagtatcat ggatcattga ttggatcaga tgagtagccc
     4141 ttgtgtgata ggtctggtag ttaagaaact caccctggaa aatatacgga gaataattac
     4201 atcacaaagt gggtcatatc tttaaagata tccacccgca tggcgatatc cagatgattg
     4261 atgcttttaa gtttggccaa ggagaagtcc tccacacaga aggatgccat taccgatgca
     4321 tatacaatgg ctctcttcat gttctcgaat gacagatctt cagttctcgc cagatagcct
     4381 atgaatcctc ctgcaaaagt atccccggcg ccggtcggat ctattacttc agacacagga
     4441 agtgccggga aggagaacat tctaccttcg cggaatagca tgacaccgtg ttcgcccttt
     4501 ttgatgataa ggtatttagg tcccatcctg tatatctttg cagcggctgc agccagggaa
     4561 tattctccac tgagctggcg tgcctcttcg tcgttgatag taatgacgtc taccatcctt
     4621 agtacctttt gaagactgtc ccatgcaaca tccatccaga aattcatggt atccagaacg
     4681 actagtttag ggcgtcgtgt catttgttgc agaacattca tctgtacttc aggagtcaga
     4741 ttgccaagca tgagataatc ggccgtacgg aaagattgtg gcagtttggg ttgaaaattg
     4801 gccagtacat ttaggtctgt aatcaaagtg tcccgttggt tcatgttgtc atggtatctg
     4861 cccgcccaga aaaaggattt ttcatcctta cgaatctcaa tgcctgagat atcaatgccg
     4921 agagaagtca tttcgtctaa ttcctgctgt ggaaaatcgc cgccgataac tgatacgata
     4981 ccttgatttt caacaaagaa tgaagctgat cggctgatgt aggtggccgc tccacctatg
     5041 actttacctg ctttccctct gggagtttct atatcgtcaa atgcgactgt tccaacggta
     5101 actaatttca tgaaaaaata attttgcaca aagattagaa aaattaaata attcatttta
     5161 gttttgccct gctttttgag agaagaggat aagaaagtta acatctttcg gtattttcga
     5221 caaaaagccg atcttgaaag ttagatcaaa gaagaaacat tcctccttag ctcagccggt
     5281 tagagtcatg gaattccatg attaatcagc gggtcggagg ttcaagtcct ggaaacaggc
     5341 ttgaaaaaga aagaaacatt cctccttagc tcagccggtt agagcatctg actgttaatc
     5401 agagggtcgt aggttcaagt cctacagggg gagcgagagg ggtcgagtaa tcggcccctt
     5461 tttttatata ggaaatcacc taaagatgca ttacttgtat atattatatt cagttagttc
     5521 agatatctat tatgtcggga taacgaataa tccggcaaag cgattaagtg agcataacga
     5581 aggcgtccat tgtacattta cctcaaaaca caggccgtgg gagatcaggg ctgtatttga
     5641 agtgggagag agtttgggaa aggcaatggt tatagagaag tttatcaaga agaacaggtc
     5701 gacccggatg atgcagttgt tgtgtgatga gtcatatgtg cctgatggtc ggctgtcggg
     5761 aatgagacga gtcattgagt attaaatcct ccttagctca gccggttaga gtcatggaat
     5821 tccatgatta atcagagggt cgtaggttca agtcctacag ggggagcaag aggggccgag
     5881 taatcggccc ttttttatta tataggaaat cacctaaaga tgtattattt gtatatatta
     5941 tattcagtta gttcagatat ctactatgtc gggataacga ataatccggc aaagcggtta
     6001 agtgagcata acgaaggtat ccattgtaca tttacctcaa aacacaggcc gtgggagatc
     6061 agggctgtat ttgaagtggg agagagtttg ggaaaggcaa tggttataga gaagtttatc
     6121 aagaagaaca ggtcgacccg gatgatgcag ttgttgtgtg ataaggattt tattccggat
     6181 ggtaaattat cgggaatgag acgagtcatt gagtattaaa tcctccttag atcagccggt
     6241 tagagtcatg gaattccatg attaatcagc gggtcgtagg ttcaagtcct acagggggag
     6301 cgagaggggt cgagtaatcg gccccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnntaggt tcaagtccta cagggggagc gagaggggtc gagtaatcgg cccctttttt
     6481 atatctaggt aaaatcgcct aaaatgtact acatcttgta atgttcagaa tactctgatg
     6541 acagtatcta ttgtagcatt atgggcaata ctttcaagtt taaaaatgta ttaaaacttt
     6601 cgatttagta tttattatta cacaaaggat agataactac tttccaatgc aaaacttgct
     6661 gaatatcgta cccaaaatat cccggtcaat gtccacgcgt ccggtcaatt ctccaatgta
     6721 tcgcaggcat tcgtgaattt ctatggcaag cagatcacca ctaagacttt cgttgaggcc
     6781 ggtttttatt ttgtcgaggc taaccgaaat tttttggaga atgtcgcgat gccgcacatt
     6841 tgtgatgatg gtttggtcag tattgatttt gcctcctact gccttttcga agatgacttg
     6901 cttgagggat tcgatcccca agccggactt ggcatcagta aggatatagg gagaatcagc
     6961 actttcgata gatgagtcgg ttgaaatgtt ttccttccat aataagtatt tcgtatagac
     7021 cgggatggtc ttggacgaaa attcctttag ccattgaaat tcatcgtaga tgttctgtct
     7081 gtcatcaaag cttacaaggc tattcatgtc catgaggtag aggatgatat ccgattgggt
     7141 ggcattgttg cgggaacgct ccataccgat actttcgatg atatcggttg tatgttcacg
     7201 gataccggca gtgtcgatga gtcggaacag gataccgttg atgttgaggg tttcttcgat
     7261 ggtgtcgcgc gttgtaccgg ctatgtcgct gacaatggca cgctcttcat tgagcagggc
     7321 attgagtagg gttgattttc cggcattggg cttgccgatg atggctacgc taatgccttt
     7381 cttgatgaca ttgcccagtc ggaacgattc cagcagggag ctggtttttt ccttgagggt
     7441 atcgatcagc acaagaaact gtttccggtc agcgaattcc acatcttcct cactaaagtc
     7501 aagttccaat tcaattaatg aggcaaattc tacgagcttt gttcgcaggt catcaagatc
     7561 tcttgagaat cctccgcgca gttgatttag tgctgtatga tgagcagcag cactttctgc
     7621 cgctatcaag tcggctacag cctctgcttg tgctaggtcc atcttgccat tgagaaaggc
     7681 acgctgagta aactcaccgg gtagcgcagc tctcgcacct gcctcgatac aaagttccag
     7741 aatgcgcgaa agg
//
LOCUS       DAOQMC010000108         7689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3853_length_7689_cov_5.004192, whole genome shotgun sequence.
ACCESSION   DAOQMC010000108 DAOQMC010000000
VERSION     DAOQMC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3853_length_7689_cov_5.004192"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="PKV31_05605"
     CDS             complement(<1..1926)
                     /locus_tag="PKV31_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92362.1"
                     /translation="MNKNLFFLVLLFVVGMANEMSALMPPGHNKPTEKQIELRELCSP
                     ATASVELNINNVRALLLNGGDVWWNRDDPRYIVPNVTVKPVSSIFAAAVWIVGYNGGS
                     IKGAGRMYGGNSNKHDWFPGPLNDLTGQITVQECSNWDRFFEVRGEEIDKHIAQFKSF
                     ELKGEDYPESLIPLNVRGWPSRGNPYFKSIYNWSLPTTTAALAPFYDYDGDGEYDPAK
                     GDFPIIEVRGVAGACESTPENPVPPTYADQMFFWIYNDNGNTHSEFNGTPINMEVQVQ
                     GYGFKTNDQLNDMTFMRYKLVNRSPTVTDKCLFGWWVDSDLGCPDDDLIGCDSTRSLV
                     YYYNIDGTDGNSGPDQTLCNAGGGSVNTYGRKVPILGIDYFEGPTIDTIINGKEVNFD
                     IGMTSFTYWNRESAEPGTVDPNNLQQLYYYMDAKWKDGTPFTYGGSGYKSGGAVTRYP
                     FSDAPTDINGWSMKTVTNLPGVDRRTAQSTGPITLQSNKTNYLVVGVPWVPDQDYTGP
                     NNAPGLNDLYAADDICQGLFDNCFKIFDGPDAPDVDVIELDRKIVLALSNDPEKSNNF
                     NLNYHGFNPGISASKDTAGMFKFQGYMIYQVVSPDISPSDYNDPTKSRLFAQVDLKDS
                     VTTLYNWTPVDNPSVTDP"
     gene            complement(1992..5603)
                     /locus_tag="PKV31_05610"
     CDS             complement(1992..5603)
                     /locus_tag="PKV31_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92363.1"
                     /translation="MSRILLLFAFLFSISVLVEAQTSLGGKIVDNESGEVIIGATVLL
                     KRSGVQTDGTITDEYGNFYFSSVAPGKYDVHITYTGYAPVIVTNVDVFNGKANKLPVT
                     KLDTETKLLQDVVIKAYKNPLIEQDNTTQGTVMTQEQIAKAPVKNIQALAAASGGVSS
                     ANGSAISIRGSRTSSTNYYIDGIRVAGGYLPPNEIEQLQVITGGLEAQYGDVTGGLIV
                     ASTKGPSSSYSVYVDGETSKFLDPYERTEINVSTSGPILKNKNNQSVIGFRIGARYLG
                     STDGSPSFDPIRYMSKDRIAQLEANPVRNIGAGGIYPEGEFNLTENTFDSKIGINEKN
                     RNIDLNGKLDFRLSNTIDAQIGFGYSDIEGKFSPSRAWQFANYENNPTSYSNRYRGNI
                     RIRHKLSTTNKGLLQNVGYVVIGGYEKLTSRNEDPRHGNKFFDYGYVGRFTSKLGEPI
                     IRLEDDEFKQVYAIIEQYDGTFIPGTQNPVLANYNNFLTPEQIINQEFITYNGQQAQS
                     NLYSTIYGLYSNVGQVYDTLSKSESDTYTGRLDINFDIVPGSSETGRHSINLGFLYEQ
                     NISRSYSLSPRNLWTLGNNLANTHLKELDYNATPIDTVDFFGNEVPIYPYKVTDQLGN
                     RFYRAVREKLGVPLSDPIFINNLSPDQLSLDMFAPGELTSYGVLGFYGYDYLGNKINN
                     ASFNDFFTKTTGSENGVRAMLLAPFKPIYQSFYIQDKFQARDVIFRLGLRVERYDANT
                     KVMKDQYSIYEISTAQEYYDQIGQQRPDNIDPDAFVYLNTAGNDASGVKAFRVGDTWY
                     TPQGVATLNPDDIFQKGITNPRYKLLPGEVNKPDIQLATYDPNRSFKDYKPQVSFSPR
                     IAISFPISEDANFFGHYDILVERPSNNYASGLSYLYWENQGSFNNPDLKPSKNIDYEI
                     GFQQKLNNSSALKLSLYYKEIRDLIQSTLILYTNNISGKMLTYRNIDFSTVKGIQIQY
                     DLRRTSNLTLQANYTLQFADGTGSDPGTQAAIAKNGQVRVLSPLDFDERHALKFTADY
                     RFDEGKSYNGPEWFGVDVFSNAGVNIITSAVSGRPYTKAATPTQFGGSGTIGTYNGQR
                     YPWNFNIDLRVDKTFRLGSVDAKRSPSINIYARVTNLLNTRNIYGLYKASGSPFDDGF
                     LPTFLGQNLYSAIDLNQVATGLGRTSAEYINAYNWLMYNPGFFNGPRRIYIGASFNF"
     gene            complement(5774..6718)
                     /locus_tag="PKV31_05615"
     CDS             complement(5774..6718)
                     /locus_tag="PKV31_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92364.1"
                     /translation="MKNVLITDDCHPDLVEGLNKAGMIVDFMPGLTLEEIDDCLYKYF
                     GIIINTRVKMDRTRIDNGPGLRFIGRMGSGLDIIDLKYAAEKGVAVISSPEANATAVG
                     EHSLSMLLSLLNKLPLGAQQVHNMSWDREAARGRQLAGSTVGIIGFGHTGPAFAEALS
                     GMRLVIKVFDKYKDVPYQKSGNSEIIPSGMEEILESCDFISFNLPLTSETAFLIDETL
                     ISKMKKGVILINAARGKLMNLDAVINGLKSGRIGGCCLDVFPNEKPATFSEQEVEQMQ
                     FLTAHPNVILTPHVAGWTFESKAKIGEVLLQKIIKNFG"
     gene            6825..7514
                     /locus_tag="PKV31_05620"
     CDS             6825..7514
                     /locus_tag="PKV31_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92365.1"
                     /translation="MFKLNKLHLFILLVTIAAIGCKKNESDDNFPRNVDIYMRYLLEG
                     NQFNGLAKVSYADTIAIGEPDTATYFFTINGKDMKQVISPGGIKNYSVKANMPFSDQY
                     EVKIKGPDHSITTKSTKIIPIDSFQLPRAISKSTALNIEYFGSPLNANESIIAMLVAS
                     DGKTASATLNGPASSKSINIKADKLSELISGLADIYLIRTYSDEDVTGKLHFFIKNEF
                     YSRTKQVELTD"
BASE COUNT         2382 a         1640 c         1368 g         2299 t
ORIGIN      
        1 aggatcagtg accgatggat tgtccaccgg agtccagtta taaagtgtag tgacagaatc
       61 tttaaggtca acctgagcaa ataatcttga tttagtagga tcattataat cagaaggtga
      121 tatgtccggt gatacaacct gatatatcat ataaccctgg aacttgaaca tacctgcagt
      181 gtcctttgat gctgaaattc caggattgaa accatggtaa ttaagattaa agttgtttga
      241 tttttccggg tcgttgctca gagccaatac aattttcctg tccaattcga tgacatcaac
      301 atccggtgcg tcaggaccat caaatatttt gaagcagtta tcaaaaagtc cctggcaaat
      361 atcatcagcg gcatataaat cattcaaacc aggagcatta tttggaccgg tataatcctg
      421 atcaggcacc catggcacac cgaccacaag ataatttgtt ttattggatt gtaaagtgat
      481 aggccctgta gattgggcag tacgacggtc aacaccgggt aaatttgtca ctgtcttcat
      541 agaccaccca ttgatatcag ttggagcatc cgaaaaagga tatcgtgtga cagccccccc
      601 ggacttataa cctgaaccac cataagtaaa aggagtacca tccttccact tagcatccat
      661 ataataatac aactgttgaa ggttatttgg gtcaacagtt ccgggttccg cactctctct
      721 gttccagtaa gtaaatgaag tcattcctat atcaaagtta acctccttgc cattgattat
      781 cgtatcgatg gttggtcctt caaaatagtc aattccaagg ataggcactt ttcgcccgta
      841 tgtattcact gaaccgccgc cagcattaca aagcgtctga tcaggtcctg agtttccgtc
      901 agtaccgtca atattatagt aatatacaag gcttcgtgtt gagtcacacc ctatcaggtc
      961 atcatcagga catcccagat cggagtccac ccaccatcca aataaacact tgtctgttac
     1021 cgtaggtgat ctgttgacca atttataacg catgaatgtc atatcattca actggtcgtt
     1081 ggtcttgaaa ccatatcctt gaacctgaac ttccatattg ataggcgtac cattaaattc
     1141 agagtgggta ttaccattgt cgttataaat ccagaaaaac atctggtcgg cataggttgg
     1201 aggcaccgga ttctcgggag tagattcaca agcacctgcg acacctcgta cttcgatgat
     1261 gggaaaatca cctttagcgg gatcgtattc tccatcgcca tcgtaatcat aaaatggagc
     1321 caatgcagct gttgtagtcg gaagactcca attatatatt gatttgaaat atggatttcc
     1381 tcttgaaggc cagcctctta cattcagagg aataagactt tcaggataat cttcaccttt
     1441 cagttcaaaa gatttaaatt gtgcgatatg cttgtcgatt tcttcaccgc gcacttcaaa
     1501 gaatctatcc caattggaac attcctgcac tgtaatctga ccggtcaggt cattcaatgg
     1561 tccaggaaac cagtcatgtt tgttactatt accgccatac atcctaccgg cgcccttaat
     1621 agatccacca ttataaccta cgatccaaac tgcagcagca aagatggacg ataccggctt
     1681 gactgttaca ttaggcacga tataacgagg atcgtcccta ttccaccaaa catcaccacc
     1741 attcaacagc agagcccgaa cattattgat attcagttca acgctggctg tcgcaggaga
     1801 acaaagttcc cgcaattcga tttgcttttc cgtaggttta ttatgtccag ggggcatcag
     1861 agcagacatc tcattcgcca tgcctactac aaaaagcaat accaggaaaa ataaattttt
     1921 gttcatttga attcttttta aatatttatc ttcaaatatc taaggttaat aaatatcagt
     1981 ttaacaatga attaaaaatt aaaactagcg ccaatgtata ttctcctagg gccgttaaaa
     2041 aatccaggat tatacatcag ccagttatat gcattgatat actcagctga cgtccttcca
     2101 agaccggtag caacctgatt caggtcaatg gctgaatata ggttctgacc aaggaaagtc
     2161 ggcaagaatc cgtcatcaaa tggtgagccg gaagctttat aaagaccata tatattcctg
     2221 gtgttcaaca aattagtcac cctcgcataa atattgatag acggagatct ctttgcatcc
     2281 accgaaccaa gtctgaatgt cttatccact cgtaaatcaa tattgaaatt ccaaggatat
     2341 ctttgtccat tataagtacc aatagtacca cttcccccga attgagtcgg tgtagctgcc
     2401 ttggtataag gtcggccgga aaccgcagaa gtaataatat tcacaccagc attggagaaa
     2461 acatcgacgc caaaccattc agggccatta tatgatttac cttcatcaaa acgatagtcg
     2521 gcagtaaatt tgagagcgtg gcgctcgtca aaatcaagtg ggctaagcac tcttacctga
     2581 ccatttttag caatggctgc ctgtgtaccc ggatctgatc ctgtaccatc agcaaactgc
     2641 aaagtatagt tggcctgcag agtcagattg ctggttcttc tcaagtcata ttgtatttga
     2701 atacctttta cagtactaaa gtcaatattt ctgtaagtaa gcattttacc agaaatatta
     2761 ttagtgtaaa gaatcaaggt actttgaatc aaatctctga tttctttgta ataaagagaa
     2821 agtttaaggg cggaagaatt attcaatttt tgctggaagc ctatttcata atctatattc
     2881 ttggatggct tcaaatcagg gttattgaaa gacccttggt tttcccaata taaataactc
     2941 aatcctgatg catagttgtt tgatggtctt tctaccagga tatcataatg accaaagaag
     3001 ttggcatcct cagagatagg gaatgaaatt gcaatcctag gtgaaaaact tacctgaggc
     3061 ttataatctt taaatgagcg attgggatca taggtagcca actgaatatc aggtttattt
     3121 acctcgccgg gaaggagttt atatctagga ttggtaatac ctttctggaa gatatcatcc
     3181 ggattcaatg tagccacacc ctgcggtgta taccaggtat cccctactct gaaagcttta
     3241 acacctgaag cgtcgttacc tgcagtgttc aggtaaacaa aagcatcggg atcaatgtta
     3301 tccggtcttt gctggcctat ctgatcataa tattcctgag cagttgaaat ctcgtagata
     3361 ctatactggt ccttcataac cttggtattc gcatcatatc tttccacacg tagtcctaac
     3421 ctaaagatta cgtcccttgc ctgaaactta tcctgtatat aaaaagactg gtagattggt
     3481 ttgaaaggag ctaaaagcat tgctctcaca ccattctcgg agcctgtcgt ctttgtaaag
     3541 aaatcattga aacttgcatt atttatttta tttcctaaat aatcatagcc atagaagccc
     3601 aaaacaccat atgaggtgag ctctccgggt gcaaacatat caagcgatag ttggtctgga
     3661 gacaaattgt ttataaaaat aggatcactc aatggaaccc ccagtttttc gcgtacagcc
     3721 ctgtaaaatc tattacccaa ctgatcggta accttgtaag gataaattgg aacttcattt
     3781 ccaaaaaagt caaccgtatc aattggagtc gcattatagt ccaattcttt caggtgagta
     3841 ttcgccaggt tattacccaa tgtccacaaa ttccttggtg aaagagaata agatctggaa
     3901 atattttgtt cataaaggaa acctagattg attgaatgtc ttcctgtctc tgaggaaccc
     3961 ggaacaatat caaagttgat atctaatctt ccagtgtagg tatcactttc tgatttgctt
     4021 aaagtgtcgt atacctgacc aacattacta tacaagccat aaattgtact atataagttg
     4081 gattgtgctt gctgtccatt ataagtaata aactcctggt taattatctg ttcaggtgtc
     4141 aggaaattgt tatagttagc aagcaccgga ttctgagtac ccggaataaa ggtgccatca
     4201 tattgttcga taatggcata cacctgtttg aattcgtcat cttccaacct aataatcggt
     4261 tctcccagtt tggatgtaaa cctgcctacg tatccatagt caaagaactt gttcccatga
     4321 cgaggatctt catttctgga agtaagcttt tcatagcctc cgataactac ataccctaca
     4381 ttctgaagca ggcctttgtt ggttgtcgaa agtttatgac ggattctgat atttcctcta
     4441 tatctgttgg agtatgaagt cggattgttt tcataattag caaattgcca tgcacgtgag
     4501 ggtgagaact ttccttctat atcagaataa ccaaaaccga tttgtgcatc aattgtgtta
     4561 gaaagccgga aatccaattt tccattcaaa tcgatatttc tgtttttctc gttaattcca
     4621 atcttagagt caaaagtatt ctcagtaaga ttgaattctc cttccggata aatacctccg
     4681 gctccgatat ttctaacagg atttgcttcc aactgagcaa tccgatcctt actcatatat
     4741 ctgattgggt caaatgaagg acttccatca gtcgaaccca aataacgtgc accaattcta
     4801 aacccaatga ctgattgatt attcttattt ttaaggatag gacctgaagt agaaacgttt
     4861 atttcagttc tttcataagg gtctaaaaat ttagaagttt ctccatcgac atatacacta
     4921 taactgctac ttggcccttt tgtagatgca acgatcaaac caccggtaac atcgccatac
     4981 tgagcttcca gaccaccggt aatgacctgc aattgttcaa tttcattcgg agggagatat
     5041 ccacctgcca ccctgattcc atcaatataa taattagtag aacttgtacg gctacctctg
     5101 atggaaattg cacttccatt ggcacttgaa acgccaccgg aagcggctgc taaggcctga
     5161 atattcttca cgggggcctt ggcaatctgt tcctgagtca tcacagtccc ctgggtggta
     5221 ttatcctgtt ctatcaaagg attcttataa gccttaataa cgacatcctg taacaacttg
     5281 gtctctgtat ccaattttgt cacgggaagc ttattggctt taccattgaa aacatccaca
     5341 ttggtcacaa tgacaggtgc atatccggta taagtaatat gtacatcgta cttaccgggt
     5401 gcaactgaac taaaatagaa attaccatac tcatctgtta tggtaccatc agtctgaaca
     5461 ccgcttcgtt tcaataaaac cgtagcccca ataataactt cgcctgactc attatcaacg
     5521 atcttacctc cgaggcttgt ctgagcttcg actaatacgg aaatactaaa caaaaaagca
     5581 aaaagtaaaa gaattctgct catatcaagt cttttatcaa attaagtttt acaagctgca
     5641 atagtaacaa aattttaaga tataaaacca attattcatc agactttatt tgtcataaat
     5701 ctaaataatg aaacttcgtt ttaaacaagc tacatctatt tatttgataa tcaaatacaa
     5761 actaattatg aatttagcca aaatttttta taattttttg taaaagaacc tctccaatct
     5821 tagctttgga ctcaaaggtc catcctgcta catgaggtgt cagaatgaca tttggatgtg
     5881 ctgtaagaaa ctgcatctgc tccacttcct gctctgaaaa ggtggctggt ttctcattag
     5941 gaaaaacatc aagacagcat cccccaatcc ttcctgattt aaggccattg atgacggcat
     6001 caagattcat taacttacct cttgcggcat tgatcagaat tacacctttt ttcatcttac
     6061 ttatcaacgt ttcatctatt aaaaacgctg tctcagaggt aagaggaaga ttaaagctta
     6121 taaaatcaca actttccagt atttcttcca tgccagatgg gataatttct gaattacctg
     6181 atttctgata cggtacatct ttgtatttat cgaatacctt aattacaagg cgcattcctg
     6241 aaagagcttc agcgaatgca ggcccggtat gtccaaaacc aattatacca acagtactcc
     6301 ctgccaactg tcgccctctt gctgcttcgc gatcccagct catattatgc acctgttgag
     6361 ctcctaatgg aagtttattc agcagtgaca agagcataga aagagagtgc tcccctacag
     6421 ccgtggcgtt ggcttcagga gaacttataa cagcaacgcc tttctcagca gcatacttaa
     6481 ggtcaataat atcaagaccg gagcccatcc tccctataaa tctcaaccct ggaccattat
     6541 ctattctagt cctatccatc ttaactcttg tgtttataat aattccaaaa tacttgtaca
     6601 ggcaatcatc aatttcttca agagtcaggc ctggcataaa atcaacaatc attcctgcct
     6661 tgtttaaccc ttccacaaga tcagggtgac agtcatccgt tatcaaaaca tttttcataa
     6721 aagcaaatgt aattcaaatg ataccataaa aatagtctta atggttagct ttgccaaaaa
     6781 ttagaccgag aataaaaaat taccgaataa tcataaaata ctccatgttc aaacttaaca
     6841 agttacattt attcatacta ttagtcacca tcgctgctat tggatgtaag aaaaatgaat
     6901 cagacgacaa cttcccccgc aatgtagata tttatatgcg ttatctgttg gaaggtaacc
     6961 agtttaatgg cctggcaaag gtcagttatg cagataccat tgctatagga gaacccgata
     7021 ccgccaccta tttttttact ataaatggta aggatatgaa gcaagtgata tctccgggag
     7081 gtattaaaaa ctattctgta aaagcaaata tgcctttttc tgatcagtat gaagtaaaaa
     7141 ttaagggacc tgatcattca attacaacaa aatctaccaa aatcattccc atagacagtt
     7201 ttcaattacc tagagctatt tcaaaatcaa ctgcattgaa tattgaatat tttggtagtc
     7261 ctctcaatgc caatgaatcg attattgcaa tgcttgttgc ctccgatggg aaaactgcct
     7321 cagcaacact taatggtcca gcttccagta aatccatcaa tatcaaagct gataagttat
     7381 ccgaattaat ctcaggctta gcggatatct accttattcg tacttacagt gatgaagatg
     7441 tgacaggcaa attgcatttt tttattaaaa acgagtttta tagccggaca aagcaagttg
     7501 aattaacgga ctgatctctt agtattatga aacctaatcc aagaaatccc tccatttagg
     7561 ttatggaatg aatggaggag taaaaagtta aaacggatat taccataacc tactggtctt
     7621 tcatagaata agtccaaggt gtatgaaatt gcaacaatag ttgaaaacca atataggtat
     7681 ttacccacc
//
LOCUS       DAOQMC010000109         7662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3872_length_7662_cov_4.744577, whole genome shotgun sequence.
ACCESSION   DAOQMC010000109 DAOQMC010000000
VERSION     DAOQMC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3872_length_7662_cov_4.744577"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKV31_05625"
     CDS             complement(<1..295)
                     /locus_tag="PKV31_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92366.1"
                     /translation="MKHDRRTFIKEFGIAAAGLCFLPSCRSILPSNPDTVGIKKIGLQ
                     LYTLRDDMPKDPKGILAQVAQAGYHQIESYEGSSGIWWGMGPKGFRQYLSDLGM"
     gene            complement(432..3092)
                     /locus_tag="PKV31_05630"
     CDS             complement(432..3092)
                     /locus_tag="PKV31_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB92367.1"
                     /translation="MNKFILLFLAIGTISMSCNSTKPAPAQTAVTAPAGQLGEEPDSL
                     EEQEGYDYTMEDDNFHNPFGVEMTLPDTLPVYRATATKLFNLIDTKLWISFDWAKRHC
                     IGTAEITATPVARVRRVLDLDAVGFDIKTVTSPEGKNLKYNYDGNTLSIDLGKDYSVG
                     SQVKVKINYVAKPDENKIGGSSAISSDKGLYFINPDGTDKNKPRQIWTQGESQSNSRW
                     FPTIDRPNQRCTQEMWITVDDNFKTLSNGVLVDSRKNKDGSRTDHYKMSQPHAPYLFM
                     MAVGEYAIVHDKWDNIPLKYYVEPKFEKDAKAIFNHTPEMLSFFSELVGVKYPWPSFS
                     QVIVRDYVSGAMENTTAVIFGEFCQRTTRELLDGNNDGIVAHEMFHHWFGDYVTCESW
                     ANITMNEGFANYAEYLWEEHKNGRDAADFHRLNEMTGYFAASRNHMHDLIYYNYDNRE
                     DVFDAHSYNKGGLVLHMLRSYLGDEVFFAGLKLYLNKNAYTAVEAANLRLAMEEVSGE
                     DLNWFFNQWYFGSGQPELTVRDSIDASGKKLFLMVEQTQEGPEQQHIFQLPVKVDIYP
                     GGNAIPLTKNIFIDKRKQTFSFDVDGTPANIIFDADNVILGKIKFDKSKEAYMYQARH
                     NKTLYDRMMALDKIKSEITPEVDSIILGYLSDPSPVIRRKALEQVDLSGFPIFTSRVL
                     ELAEKDKEPSVRATALAILAENPKPEYEKTIVSNINDEVAYSVLASALSAYNKLDKDK
                     AEPFISKYEKENNSTITSVIAEIYKSNNDPRALTLMEGKLASSEGFEAFGIFGNYTDL
                     LLDRDKSTIIKGFETIEKMAKSSEFSQWKKFAVAKSLFDIAGATQSNPSFSEQESSDL
                     VKRSNAIVQSFIDKAGNERMKDAMSNLLIK"
     gene            complement(3133..3948)
                     /locus_tag="PKV31_05635"
     CDS             complement(3133..3948)
                     /locus_tag="PKV31_05635"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNB92368.1"
                     /translation="MDQLKRTIEAAWDNRDLLNLEETRKEIRTVIELLDKGKLRVAQQ
                     EGDDWIVNEWVKKAVILYFPIQQMETIEVGPFEWHDKIALKKSFAEQGVRSVPHAVAR
                     YGSFLEAGVIMMPSYVNIGAYVGAGTMVDTWATVGSCAQIGSNVHLSGGVGIGGVLEP
                     VQASPVIIEDDAFIGSRCIVVEGVKVGREAVLGANVVLTQSTHIIDITGPEEVRYKGV
                     VPPRSVVIPGSYSKIFPSGTYQVPCALIIGKRKESTDKKTSLNSALREYNVAG"
     gene            5275..5745
                     /gene="mraZ"
                     /locus_tag="PKV31_05640"
     CDS             5275..5745
                     /gene="mraZ"
                     /locus_tag="PKV31_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNB92369.1"
                     /translation="MQLYGEYECSVDEKGRLKLPVALLRHVGGNPPYKFYVNRGFEKC
                     LMLYPEKVWDEIVEKVNNLNSFVTEERNFIRYFFRGLRDVATDAAERILLPKPLIEFA
                     GIKKEVVLFGYMGRVEIWDKATYYDWADVEPDNFAQLAEKLLGNKREEENPTGL"
     gene            5745..6650
                     /gene="rsmH"
                     /locus_tag="PKV31_05645"
     CDS             5745..6650
                     /gene="rsmH"
                     /locus_tag="PKV31_05645"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNB92370.1"
                     /translation="MQEYHLPVLPRETIEILDIKPSGIYVDATVGGGGHSKLILERLN
                     DKGRLYVMDQDLDVVGNIPNDDRVIFIHSNFRYLRRMLRVEGVSLVDGILADIGVSSH
                     QFDTGERGFSFRFEGDLDMRMNRNREFTAADVLSNYDEAALVEMFSSLGEVRNAKTLA
                     KLIVDIRSKRPIKTIEEFVYGIQPAIMGNAAKYLAQVFQALRIEVNDELKALEDLLFD
                     AVEVLKPGGIMAVITFHSLEDRIVKNFFKTGNPEGRVVKDEYGHIFRPLIPITKKPIE
                     AGKEELMVNQRARSAKLRAARRSGD"
     gene            6771..7085
                     /locus_tag="PKV31_05650"
     CDS             6771..7085
                     /locus_tag="PKV31_05650"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNB92371.1"
                     /translation="MKKLRKQRQNWIDDFGMDILKRNTPFICFLGLLGLLYIANTHSA
                     ENKVNKILALKTEIRELNWEYLKLKSELVHNSMYSTVESKLSVQELGLRGQKPVQVIV
                     KD"
     gene            7088..>7662
                     /locus_tag="PKV31_05655"
     CDS             7088..>7662
                     /locus_tag="PKV31_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92372.1"
                     /translation="MSAPVDNKSEVVRNSNIVLWIFSALAVLIIAQVINIGFVKGEHY
                     RTLGKDLRIKAIDIQADRGNILSDNGELLAVSMPFYDVYFDPTASRQYVKEMKEKDRY
                     FMKKTGDIFAKNIDSLSICISKYLKPNLSPNEIKRWLISKRNQNERYLCLAKGISHEK
                     MKLVKGFPLFRMGKNKSGVIFEMKQSRVRPYG"
BASE COUNT         2165 a         1420 c         1716 g         2361 t
ORIGIN      
        1 ccatgcccaa atcggataaa tattgcctga aaccctttgg tcccatgccc caccatattc
       61 ctgaagaacc ttcatagctt tctatttgat gatatcctgc ctgggcaact tgtgccaaaa
      121 taccttttgg gtctttgggc atatcatcgc ggagtgtgta taattgaagt cctatttttt
      181 taattcctac agtatcggga tttgagggca gaatacttct gcacgaaggt aaaaagcata
      241 agccggctgc tgctatacca aattccttta taaatgttct tctgtcatgc ttcacggttc
      301 caaagattta aattagtgta aatttagtat ttaaacttga gttaatgaag ccaacgggaa
      361 tataatacac tcaaaaaaag tctccatcgc tcttgcggtg gagattcatc cgactatata
      421 tttgttgttt tttatttaat taaaagattg ctcatcgcat ctttcatcct ttcattaccg
      481 gctttatcaa taaagctctg aacaatggca tttgaacgct ttaccaggtc agaagattcc
      541 tgttccgaaa aggaagggtt ggattgcgtt gcgcctgcta tgtcaaaaag cgatttagca
      601 accgcaaatt ttttccattg tgaaaattca gatgatttgg ccattttttc tattgtttcg
      661 aatcccttga tgatagtgga tttatctctg tccaacaata aatctgtata attgccgaag
      721 atgccgaatg cttcaaaacc ttcggaggag gcaagtttac cttccataag tgtcaaagcc
      781 cttggatcat tgttggattt gtatatttct gcaatgacag acgtgatggt gctgttattc
      841 tccttttcat atttgctgat aaaaggttct gccttgtctt tatctaattt gttataagct
      901 gagagggcgg atgcgagtac cgaataggcc acttcgtcat tgatatttga cacgatcgtt
      961 ttttcgtatt caggcttagg attctctgct agtatcgcca aagcggtagc cctaacggat
     1021 ggctccttgt ctttttctgc aagttctagt accctggagg tgaagatagg gaaaccggac
     1081 aggtctactt gttccaacgc ttttcttctt atgacaggag atgggtcaga caaataaccg
     1141 agaatgatac tgtctacttc aggtgtaatt tcggacttga ttttgtccaa tgccatcatt
     1201 ctgtcgtaca gcgttttgtt gtgtcgtgcc tgatacatat aagcttcttt tgatttgtca
     1261 aatttgattt tgcccaaaat tacattatca gcatcaaaga tgatattggc aggtgtgcca
     1321 tccacatcaa aggagaaggt ttgttttctc ttgtcgatga atatattttt ggtcaatggt
     1381 atggcatttc cacccggata gatgtccacc ttgaccggaa gctggaagat atgctgttgc
     1441 tcaggtcctt cctgggtttg ttctaccata aggaacaatt tctttcctga tgcgtctatg
     1501 ctgtccctta ccgtaagctc gggttggccg cttccgaagt accactgatt gaagaaccaa
     1561 ttgaggtctt caccagatac ttcttccata gccagtctca ggttagctgc ttcgactgcg
     1621 gtataagcgt ttttattgag atatagtttg aggccggcaa aaaatacttc atcgccgagg
     1681 tacgaacgta acatgtgcag aaccagaccg cctttgttgt aggagtgagc atcaaacaca
     1741 tcctctctgt tatcgtagtt ataataaata aggtcatgca tatgattcct tgatgcagcg
     1801 aagtatcctg tcatttcatt caacctatgg aagtccgcag catccctgcc gtttttatgc
     1861 tcttcccaaa ggtattccgc atagttggca aatccttcat tcatagtaat attggcccat
     1921 gattcgcacg tcacataatc accgaaccaa tgatggaaca tttcgtgagc aacgataccg
     1981 tcattgttac catcgagaag ctctcgtgtt gtcctttggc aaaattcgcc aaaaataaca
     2041 gccgtggtgt tttccattgc gccggagaca taatcccgga caataacttg agaaaaactc
     2101 ggccatggat atttgacgcc cactagctct gagaagaatg acaacatttc cggtgtatga
     2161 ttgaagatag ccttagcatc cttttcgaat ttaggctcga cataatattt aagaggaata
     2221 ttatcccatt tgtcatggac tatggcatat tcacccactg ccatcatgaa aagatatggt
     2281 gcgtgaggtt gtgacatctt gtaatgatca gtacggcttc catccttatt tttcctggaa
     2341 tcaactaaca cgccattgga aagggtcttg aaattgtcat caacagtgat ccacatttct
     2401 tgggtacatc gctgattcgg cctgtcgatg gtcggaaacc acctgctgtt actttgggat
     2461 tccccttgtg tccatatctg acgcggcttg tttttatcag tcccatccgg attgataaag
     2521 taaagtcctt tgtcagagct tattgcagaa gaacctccaa ttttgttttc gtccggtttt
     2581 gcgacataat tgattttaac cttaacctgt gatccgactg aatagtcttt gccgagatca
     2641 atcgacaggg tattgccatc atagttgtat ttcaggtttt taccttcagg ggaggtgaca
     2701 gtcttgatat cgaaacctac tgcatccaga tccaaaactc ttctaaccct tgccaccgga
     2761 gttgcagtga tttcagccgt accaatacag tgccttttag cccaatcaaa actgatccat
     2821 aattttgtgt ctatcagatt gaaaagtttt gttgctgtgg cacgatatac gggaagggta
     2881 tcaggcaaag tcatttccac tccaaatgga ttgtggaaat tatcatcttc catggtataa
     2941 tcgtagcctt cctgttcttc cagggaatca ggttcttccc caagttgtcc ggcaggtgcc
     3001 gtaactgcag tctgcgcagg tgcaggcttg gtagaattgc atgacatgga aatagtccca
     3061 atggcaagaa ataatagaat gaatttattc actttatttt ggttttatgt taagttaata
     3121 taataactaa tatcagcctg ctacattgta ttctctaaga gctgagttga gactggtttt
     3181 tttatcggta ctttccttcc gctttcctat gataagggcg caaggtacct gataggtgcc
     3241 cgatgggaat attttggaat atgaaccagg gatgacgacg gatcttggag gtaccacacc
     3301 cttatagcga acttcttcag gtcctgtgat atctattata tgtgtgcttt gggtaagaac
     3361 gacattagct cccaatacag cctccctgcc aacttttacc ccttccacga caatgcacct
     3421 ggaacctatg aaggcatcgt cctctatgat aacaggactt gcttgtaccg gctccagaac
     3481 cccaccgatg cccacgccac cactgagatg gacattgctc ccgatttgtg cacatgaacc
     3541 tactgttgcc catgtatcga ccatggttcc agcgccgaca taagcaccta tattgacata
     3601 ggagggcatc atgatgacac cggcctccaa gaagcttccg tagcgggcca cagcatgggg
     3661 taccgaacgg acaccttgct ccgcaaatga tttcttcaat gctattttat cgtgccattc
     3721 gaacggccca acttcaatgg tctccatttg ctgaattggg aaataaagga ttactgcttt
     3781 tttgacccat tcgttcacta tccaatcatc accttcctgt tgagcaaccc taagtttccc
     3841 tttgtcaagt aactctatga cagtgcggat ttcttttctg gtctcttcca ggttaagtaa
     3901 gtcacgatta tcccaggccg cttcaatagt tctttttagt tgatccaatg ttgtttagtt
     3961 ttgagtcacg aagataggat tttatcaaag aaaataaaat aagttccctg aaatagcgga
     4021 tttggtcgat ggggagattg ataattagga caaagtagca ggtggaatta taccgcatag
     4081 gccttacggt aaatattaaa gcggagcgga ttgtaggaga ggatattctt tcaattgatg
     4141 tggtgtctga tcaatatgag atattatagg ttttgaaaaa gggggggaaa caaggttgga
     4201 gtttttggtt agggaaaaac gaactaggta gaagattaat accccgggat tcagtgatgc
     4261 cgggttgttt gaggtatgag gttttgaaat ttttccctga tgatgtaatt ttttggattg
     4321 gaggtgtcgg gataccggtg gttgtaggta ttttgagtat taattatcta cctacttcaa
     4381 attttcgacc gggcgtcctt cgaatagcta tttggtacgt acatatcagt gacatttcat
     4441 ggggttaggt tgaattttga aaaaaaaacg aactaggtag aagattaata ccccgggatt
     4501 cagtgatgcc gggttgtttg aggtattaag ttttgaaatt tttccttatg gatgtaattt
     4561 tttggattgg aggtgtcggg gtaccagtag ttgtaggtat tgtgagtatt aattatctac
     4621 ctacttcaat attaggcaat acccataccg aatcacattt tttttgatgt tttttttgat
     4681 tgaggatcct tataggattt tattaagtgg aattcggatg aatttcattc aggtgaaccg
     4741 actggaaaga tataaaggta gtttaattga ggaggcacta ttgcatatta tatacttccc
     4801 acaaacgtga cccaaaagcc caaaatccgg acagtcaatt attcacggaa gcttaatcgg
     4861 gttagcactg accaatgtat ttaatattaa tatactatcg agctcatctc tttactcatg
     4921 tttggtgtat gcatgatggt atactttccg gttaaaaaaa ccaggttgat ggcatgatgt
     4981 gcagatttta ttgaaataat tcccataatt taccacttgg ctgtagatta gattgagaat
     5041 aaagtataat acttattata ttgtaaacca tatgtattaa tatatagata atttaaatat
     5101 tataatatgt aatcaaaata tttacaaaat aacattattg tggttatctt catatatgaa
     5161 aaataataat ttgatataaa taataaattt tataggggaa aaatgtgtca gtaggtggga
     5221 gtttgtatta cttttgacac agaagaacag aaaatagttc ttggaaccta attaatgcag
     5281 ctatacggag aatacgaatg tagcgttgat gagaagggca ggttgaagtt gcctgtcgct
     5341 ctgttgcgcc atgtgggtgg aaacccgccg tataagtttt atgtcaatcg gggatttgag
     5401 aagtgtttga tgttatatcc cgagaaagtt tgggacgaaa tcgttgaaaa agtgaataac
     5461 ctcaactctt tcgttacaga agagcgcaac tttatccggt attttttcag aggccttagg
     5521 gatgtggcga ctgatgcggc tgaacgcatc ctccttccca agccattgat tgaatttgcc
     5581 ggaattaaaa aagaagttgt gctgtttggg tacatgggtc gtgtagagat ctgggataag
     5641 gctacttatt atgattgggc ggatgtcgaa ccggataatt ttgctcaatt ggctgaaaaa
     5701 ctccttggta ataaacgtga agaggaaaat ccaaccggat tgtgatgcaa gaatatcatt
     5761 tgccggttct gccccgggag actatcgaga tactggatat taagccatcc ggtatttatg
     5821 tcgatgcaac cgtgggtgga ggagggcaca gcaagctgat tcttgagaga ctcaatgata
     5881 aaggtcggct ttatgtgatg gaccaggatc tggatgtagt gggtaatatt ccgaatgatg
     5941 acagggtaat ctttatccat agcaattttc ggtatctgag gagaatgctt cgtgtagagg
     6001 gtgtgagtct ggtggatgga attttagctg atattggagt ttcgtctcat cagttcgata
     6061 caggagaaag agggttttca ttccggtttg aaggtgatct ggatatgcgg atgaacagaa
     6121 atcgagaatt tactgcagct gatgtcttaa gcaattatga tgaagctgct ttagtcgaga
     6181 tgttttccag tctgggtgag gtaaggaatg caaagacgct tgctaaattg attgtggata
     6241 taagaagtaa gcggccgatt aagaccattg aggagtttgt atatggtatt cagccggcaa
     6301 tcatgggcaa tgcggcaaaa tatctggctc aggtttttca ggccctcagg attgaggtga
     6361 atgacgaact gaaggcactg gaggatttgc tattcgatgc tgtagaagtg ttgaagcccg
     6421 ggggaatcat ggctgtgatt acttttcata gtcttgaaga caggatagtc aagaattttt
     6481 tcaagacagg taatccggag ggcagggtag taaaagatga atacggccac atattcagac
     6541 ctttaatccc gataaccaaa aaaccgattg aggcggggaa ggaagaatta atggtcaatc
     6601 agcgtgcaag aagcgcaaaa ttaagagcgg cgagaagaag cggagattaa aatctccctg
     6661 tggccacaag gtaatgtggc agaaaaggta taagaaaaca actatttata ttcttgtgta
     6721 aggtccaacc gtgcacaggg gattaaggtc tcaaaattag aaactacaaa atgaaaaaac
     6781 tacgtaaaca acgccaaaat tggattgatg attttggcat ggacatcctg aaaaggaaca
     6841 ctccttttat ttgctttctc gggttacttg gcttgcttta tattgccaat acacatagtg
     6901 ccgagaacaa agttaacaag atcctggcct taaagacgga aatcagggag ctcaattggg
     6961 agtacctgaa acttaaaagt gaactcgtgc ataatagcat gtattcaact gttgaatcca
     7021 aattgtctgt tcaggaactg ggcctgagag gccagaagcc tgttcaggtc atcgttaaag
     7081 actaaggatg tccgctcctg ttgataataa gtcagaagta gtaagaaatt ccaatattgt
     7141 gttgtggatt ttcagtgctt tggcggtgtt gatcatcgct caggttatta atattggctt
     7201 cgtcaaggga gagcattaca ggactttggg taaggatttg agaatcaagg ctatcgatat
     7261 ccaggcagat agaggtaata tcctgagtga taatggtgag ttgcttgcag tatccatgcc
     7321 attttatgat gtttacttcg atccgactgc ctcaagacaa tatgtaaagg agatgaaaga
     7381 aaaggatcgg tatttcatga agaaaaccgg tgacattttt gcaaaaaata ttgactcttt
     7441 atctatttgc atttcaaaat accttaagcc caatctaagt cccaatgaaa tcaagagatg
     7501 gctgatttca aaacggaatc aaaatgaaag atatttatgt cttgctaagg gcatttcaca
     7561 tgaaaagatg aaattggtca agggcttccc actttttaga atgggcaaga ataagtcggg
     7621 agtcattttt gaaatgaaac aaagtcgtgt tagaccatat gg
//
LOCUS       DAOQMC010000110         7574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3947_length_7574_cov_5.883761, whole genome shotgun sequence.
ACCESSION   DAOQMC010000110 DAOQMC010000000
VERSION     DAOQMC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3947_length_7574_cov_5.883761"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKV31_05660"
     CDS             <1..336
                     /locus_tag="PKV31_05660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB92373.1"
                     /translation="KPEQVFVVTKGGLGNVKPVISRAESLKVDVDFVITRAVASLKDL
                     VTWSMKHISGKSAHAVPNGLLALKGPAFREEAKLLPKSDYMESYAIGKWFPEAYFEEK
                     YLVYIQGRR"
     gene            complement(931..1698)
                     /locus_tag="PKV31_05665"
     CDS             complement(931..1698)
                     /locus_tag="PKV31_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92374.1"
                     /translation="MKKIKIHTLLLLVAVSFYSCSKTDNDNSLLEPAFQRCHLSKEYI
                     KNVLYREVIYRDNSYQVDHILYYQNNVVREDWTEYFEYENGRIASIKDDYNSTFYKYN
                     DRGKLVSVKYCGSSVNDCCTGTYSYNATNTIPVSIAYACSDGRVSSEVFDYTNYGNLS
                     YYYIYSEQNQSVVQLYQKFTEGYISPFEEIYPKGVDYFQDRIVEDYKARRFKEYTIDP
                     KDVKGRYPLKMSVSTYSLPDFTQLSTDVLTYEYVGCD"
     gene            complement(1751..2431)
                     /locus_tag="PKV31_05670"
     CDS             complement(1751..2431)
                     /locus_tag="PKV31_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92375.1"
                     /translation="MKTTDNFCIWDMYKCPLHGWLLLLLMSLPIICVGQEGYQRSVNK
                     GFSFRTSIMADVWKGKVVSKKYTTTIGPDFTASFGLSENIAVFGSYQYLFSSKLSPVE
                     VGFLLFTDKVKHQNFAFGVKYIAGSTSSRLRYMAEAGGLIGHSTINIYQELYGNFVDI
                     KLKGLGFHSGAGIEYFISPFLSCDATLGFNIGKYKSSEYLGVSYKESLNWNRIQIMLG
                     IHYHFAGR"
     gene            complement(2437..4152)
                     /locus_tag="PKV31_05675"
     CDS             complement(2437..4152)
                     /locus_tag="PKV31_05675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB92376.1"
                     /translation="MNISEVFHSRYELLQRVGRGGFSEVWKALDMRSGVEVAIKVFRQ
                     QDSEGIALCREEYLKTFDFQHPNILTPFHFDVSDDRPYLVMKFLNGGTLADHLGNMNA
                     AKVEDLIRQLGSALKYLHSRPVPVIHGDIKPDNILIDENGNYFLTDFGISTKLIQRFT
                     ETLRADSIVDSSKGVTPMAYRSPESFKYKNWEFNKTGPFSDIWSAGVTVYQAIYNSLP
                     FNGEGGLGQLILMKSGNHSLEEIIEFEDGDFTRFNAIILAALQLNPQQRATFLGEVVD
                     SPEPIITSESISDPPLLIDDTEGISIRKKKESRAFLYPLLAIFAIGVVLLVIALSKGG
                     KEKEGNNPPALSQEAIQTVDSIQDQTVDNSDHNVNDKLHATRDISTDEKIAANLRTEA
                     KTVPVNNSSPAIQTNTPEENHTTPIDQSENVSSAKENIQSEKAEPVSVETRIMNPSES
                     REKTKSRSVTIKPNIPIPLMLNEDIGNPEALSSGSKISFVVADDVLSYGDVFLRKGQK
                     VQAVVKKVTRDKIKIHFPDIYTTGNTKLKSPNLDNFDIKIGKDQKGIIFYPVTSSYQY
                     NVNVK"
     gene            complement(4155..5411)
                     /locus_tag="PKV31_05680"
     CDS             complement(4155..5411)
                     /locus_tag="PKV31_05680"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNB92377.1"
                     /translation="MLFQFEAIVDIGLSRSVQQDNTLTMPQLILNNQCLTDEYFYDDI
                     NGALFAVADGMGGGPHGELASRLVVDGLRHFAVCNKHLLESNVITFTNNAIYEANQRL
                     LDHLNEHPEDNRMGSTVVLAIVRNGVLYLGWVGDSRCYLVNGNSIKVLSHDHSLVQSL
                     IDEGSITEEEAFYHPNRNIINQSMGMSNIIPSFEKYSLEDNQILLLCSDGLNGMLRDF
                     EILEICNRHSDLKVMVQSLVDAANKAGGHDNISCIGIRLKDYPARAKIFIKNEHPAGS
                     KKSENKIPLKLILLSIFLIIAFIWVFLVLKSGNDTSLQENDRIHSRQTMPVSPDTTAE
                     EDIGMPVESQGFYFIRLKGFNDSLKAQEYLEQLSNQYNNYVFMINYSSNALYEVTVNG
                     FKDEDEANKFIASGQFPSAIIKLQKE"
     gene            complement(5432..6169)
                     /locus_tag="PKV31_05685"
     CDS             complement(5432..6169)
                     /locus_tag="PKV31_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB92378.1"
                     /translation="MNTRFTNFILTIILTTALGLILTIFYSFFPNRLLKLLGGEVPYG
                     LIQLATYFFFIFGMLEVLRFKKFVEFQKKGLSLNLLPEKEQFVISADEVNDIKLKMIE
                     LEKSNPLLLVDMIKKACTKFRSNKNVSESLSIVSVQTEINLRNTDSEQNLIKYFAWAC
                     QSIGLIGTVIGIGASLRAANQISSQEGIDYVTGLLSVSFDTTLIALVLSIILMYAYSL
                     ISERIDRLHADNQIYVIENLINRIYNK"
     gene            complement(6201..7184)
                     /locus_tag="PKV31_05690"
     CDS             complement(6201..7184)
                     /locus_tag="PKV31_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92379.1"
                     /translation="MRRAKEFHLVNISFIDMLAGALGATIILFIIVPKMSFFDLKKLK
                     ALDSLDVKKAHMDSMVAKLESIVPEKEYRDLVASSVSLQSSIENLKSEIRTLQNTLNV
                     KTIQYSHVAQKYDRAMQTIRTIQQQLAGNPDDEELKRIQAELKAAQTALSPGPTDVSL
                     VANGKQSSPGITPPDASLSRGDAAMGKEPPLIILINWDDKDDKVHLYMRSAGTSNWVF
                     YQNNHRKAGFGTWDDNLANYEDLPYEAIVQNDNLVPGTYEIYAQTASAKTGSVEVSGF
                     ISMKINDGPVKRFNILSRKINVAKPPFEDNSGSTKLGTLTVSGDDIGWNPN"
     gene            complement(7207..>7574)
                     /locus_tag="PKV31_05695"
     CDS             complement(7207..>7574)
                     /locus_tag="PKV31_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB92380.1"
                     /translation="DEKQPVKSSFTLQILDGEEYDRQYHIRLDGFARMGRATSFNDNE
                     IEIMETISNYISRRHATFETIGSAVYLRDGQWIEDGGNWKFSKNGTYVNGLKIDDEEG
                     FQLYQNDIITIGNTSIKII"
BASE COUNT         2228 a         1561 c         1439 g         2346 t
ORIGIN      
        1 aagccggagc aggtctttgt ggtgaccaaa ggcggtctgg gcaatgtcaa accagtgatt
       61 tcacgggctg aaagtctgaa ggtcgatgtg gactttgtaa ttacccgcgc tgtggcgagt
      121 ctaaaggatt tggtcacctg gtcgatgaag catatttcag gcaagtctgc acacgccgta
      181 cccaacggat tgctggcgtt gaaaggaccg gcctttaggg aagaagccaa gttactaccc
      241 aaaagtgact acatggagag ttatgccatc gggaaatggt ttccggaggc ctatttcgag
      301 gaaaagtatt tggtgtatat ccaggggagg agatgaatcg atgataaccc tattccaatt
      361 ttgaagcctt caacttttta tcccccatac gcatggtttt cctccatccg cttttgattg
      421 ccctttaaag tatagctaaa gaggcaaggg tacaccatac gttttaatct aagttgcgct
      481 tttgcggatg aaatcctgcg cagattgtaa ggttccggag ggtggtgttc aattttgtgt
      541 actacggatt ttttcctgac ttatatcgtt tagtgtggtg ataaaatttt tgaaatcact
      601 ctttttcatt gatcagttgg gtgagcttcg atagatgctg ctttgttgga tacttgattg
      661 taaaggatac catctgtaat tttttggctc cccaaatatt tcaaaattaa gcaatatgca
      721 cttttttgca cattattgca aacaatccaa catattgtta tccttccaaa ttctttccat
      781 tgaatgcatc aagtaaaagt ctgtagaaaa acagaataga ggtcagataa agaaagaact
      841 gaatctgtcg gtcagaactt tccggcgcaa taccttgtat cattcaggtt acagcatcaa
      901 cattatcacc tgccaaaata taagtggaca tcaatcacag ccgacatatt cataggtaag
      961 aacatccgtg ctcaactgag tgaaatcagg aagagaataa gtgcttaccg acatttttag
     1021 cggatatctt cctttaacat cttttggatc aatagtatac tccttgaatc ttctcgcctt
     1081 ataatcctca actattctat cctgaaaata atctacccct ttggggtaaa tttcttcaaa
     1141 agggctgata tagccttctg taaatttttg gtataactga acgacagact gattctgttc
     1201 actataaata taatagtagg acagattgcc ataattggta taatcaaata cttctgaaga
     1261 aactctcccg tcagaacagg catatgcaat actcaccgga attgtatttg tagcattgta
     1321 agaatatgtt cctgtgcaac aatcatttac agagctgcca caatatttga cagataccaa
     1381 tttaccccgg tcattatact tgtaaaaagt cgaattataa tcatctttaa tggatgctat
     1441 tcgtccattc tcgtattcaa aatattcagt ccaatcttcc cgaacaacat tattctggta
     1501 gtaaagaata tggtcaacct gataactatt atcacgatag atcacctccc tgtaaagaac
     1561 atttttgatg tattcctttg aaagatgaca cctttggaat gcaggctcaa gcagcgaatt
     1621 atcattgtcg gttttactac atgaataaaa ggaaactgca accagaagaa gtaaggtatg
     1681 gattttgatc tttttcatct tgatagaaat attttatttg aaattaaaga aatgtaacat
     1741 tcaatggcga ttatctgccg gcaaagtgat agtgaattcc caacataatc tggattctat
     1801 tccaattcaa cgattcctta tatgaaacac ccaggtattc ggaagatttg tactttccta
     1861 tattgaatcc gagggtagca tcacaagaaa gaaaaggaga aataaagtat tcaataccgg
     1921 cgccactatg aaaccctaat ccttttaatt tgatatccac aaaattgcca tacaattcct
     1981 ggtaaatatt tattgtactg tggccaatca gtccgcctgc ttccgccata tatctcaacc
     2041 ggcttgaggt agagccagca atatatttga caccaaatgc aaaattctga tgtttgactt
     2101 tatccgtaaa caacagaaat ccgacttcaa cgggacttag ctttgaggag aataaatact
     2161 ggtatgaacc gaataccgca atattctctg acagcccaaa agaagccgtg aagtccggtc
     2221 ctatggtcgt agtatacttt tttgaaacca cttttccttt ccagacatca gccataatgg
     2281 atgtcctaaa gctaaacccc ttattgaccg atctttgata accctcttgt cccacgcaaa
     2341 taatcggcaa agacattaag aggagaagta accatccatg aagcgggcat ttgtacatat
     2401 cccaaataca aaagttatca gttgttttca tgacacttat tttacattta cattatattg
     2461 atagcttgaa gtaaccggat aaaaaatgat tcccttctga tcctttccaa ttttaatatc
     2521 aaaattatcc agattgggac ttttcaattt tgtattgcct gtggtataaa tatccggaaa
     2581 atgaattttt attttatctc tggtcacttt tttgacaact gcctgaactt tttgtccttt
     2641 gcgcaagaag acatctccat aagacaaaac atcatccgcc accacaaacg aaatcttgct
     2701 accggagctc aaagcctccg gattgcctat atcctcgttg agcatcaatg gtatgggtat
     2761 attgggcttt atagtgactg acctggattt agttttctcc ctgctctctg atggattcat
     2821 tattcgtgtc tcgactgata caggctcggc tttttcagac tgaatgttct ccttggcgga
     2881 agagacattt tcagactgat caatcggagt tgtatgattt tcttccggtg tattagtctg
     2941 tatggccggg gaagaattat ttacaggaac ggtttttgct tcagtcctca aattggcggc
     3001 aattttctcg tcggtggaaa tatcccgggt agcatgtaat ttatcattaa cattatgatc
     3061 actattatca acagtctgat cctgaattga atcaacagtc tgaatagctt cctgtgaaag
     3121 cgccggtgga ttgtttccct ctttctcttt gcctccttta gaaagggcaa ttaccaataa
     3181 aactacacca atggcgaata ttgcaagcaa gggataaaga aaagcacggg attctttctt
     3241 ttttcttatt gaaatgcctt ctgtatcatc aatgagtaga ggcggatctg atattgattc
     3301 tgatgtgatg ataggttccg ggctatccac aacttcaccc aaaaaagtag cacgttgttg
     3361 aggattcaat tgtaatgcag ccagaataat tgcgttgaag cgagtaaaat cgccatcttc
     3421 aaactcaatg atttcctcca gcgaatgatt accacttttc atcagaatca gctgtcccag
     3481 accaccttca ccattaaaag gcaatgaatt ataaatagcc tgatacaccg taacacctgc
     3541 tgaccaaata tcggaaaaag gcccggtctt gttaaattcc cagtttttat acttaaatga
     3601 ttccggactg cggtatgcca tcggagtaac tcctttactt gaatctacaa tagagtctgc
     3661 tctaagagtt tcggtaaatc tttgtattaa tttggtagaa ataccgaaat cagtgagaaa
     3721 gtaattacca ttttcgtcta tgagaatatt atcgggttta atatccccgt gaatgacagg
     3781 aacagggcga ctgtgtagat atttcagtgc cgagcccaat tgacggataa gatcctcaac
     3841 ctttgcagca ttcatatttc ccaagtgatc ggcaagtgtg cctccgttca gaaatttcat
     3901 gacaagataa ggccgatcgt ctgagacatc gaaatggaaa ggggtaagaa tattaggatg
     3961 ttgaaaatca aaagtcttga ggtattcttc tctgcacaag gcaataccct ctgaatcctg
     4021 ttgcctgaac accttgatgg cgacctccac cccggaacgc atatcgagcg ccttccatac
     4081 ttcagaaaag cctcccctgc caaccctttg gagcaattca taccttgaat gaaacacctc
     4141 tgatatattc attgtcattc cttttgtaat ttgattattg cactgggaaa ttgtccggat
     4201 gcaatgaatt tatttgcttc atcttcatcc ttgaagccat tgacagtaac ctcgtagagt
     4261 gcgtttgaac tatagttgat cataaaaaca tagttgttat attgatttga tagttgttcg
     4321 agatattctt gtgctttcaa gctatcatta aaccctttca gcctaataaa atagaatccc
     4381 tgagattcaa caggcatacc gatgtcttcc tctgcagtag tatcaggtga gacaggcatt
     4441 gtttgccggg aatgaattct gtcattttcc tgtaaagaag tatcgttacc agattttagt
     4501 actagaaaga cccaaataaa ggcaatgatt aaaaaaatac tgaggaggat taatttgagg
     4561 ggaattttat tctcactctt tttacttccg gcaggatgtt cattcttaat gaaaatctta
     4621 gccctggcag gataatcctt taaccgaatg ccgatacaac taatattgtc atgtcctccc
     4681 gctttgttgg cagcatccac cagagattgg accataactt tcagatcact gtgtctgttg
     4741 catatttcaa ggatttcaaa atctcttagc ataccattaa ggccatccga gcacaataaa
     4801 aggatttgat tatcttctag gctatatttt tcaaaggatg gaataatatt tgacatgccc
     4861 atgctttgat tgatgatgtt tcgatttgga tggtagaaag cctcctcttc tgtaatagag
     4921 ccttcatcta tgaggctctg caccaaggaa tgatcgtgtg acaatacttt gatgctgttt
     4981 ccattgacta aataacaacg actatcgcct acccagccta aatacagcac accattcctg
     5041 acaatagcaa gtaccaccgt tgaccccatt ctattatcct ccggatgttc attcaggtga
     5101 tccagcagcc tttggttagc ttcatagatg gcattattgg tgaaggtaat cacattactc
     5161 tccaggagat gtttgttaca aacagcaaaa tgccggaggc catcaacgac caatctggat
     5221 gctaattctc cgtgaggtcc accgcccatg ccatctgcga ctgcaaagag agcgccattg
     5281 atatcatcat agaaatattc atctgtaagg cattggttgt tgagaatcaa ctgaggcatc
     5341 gtcaacgtgt tatcctgttg aacagatcgg cttaatccga tatcgacgat ggcttcaaat
     5401 tgaaatagca cgactaaatc atttatggca gttatttgtt atagattcga ttgatgaggt
     5461 tctcgataac atagatctgg ttgtcagcat gtaatctatc aatcctttct gatatcaggc
     5521 tgtaggcata catgagaata atgctcaaga caagagctat caatgtagtg tcaaatgaaa
     5581 cactcaacag accggttaca tagtcaatcc cttcctggct gctgatttgg ttggccgctc
     5641 tgagtgaagc accgatacct ataacagtac cgatgagtcc tatagattgg catgcccatg
     5701 caaaatattt aatcagattt tgctctgaat cggtattcct cagattgatt tcagtctgga
     5761 ctgaaacgat actgagtgat tcgctgacat tcttattgga ccgaaacttg gtacaagcct
     5821 tcttgatcat atccacaagg agaagagggt tagatttttc caattcaatc atttttaact
     5881 tgatatcatt gacttcatct gctgatatga cgaattgttc cttttccggc aacaaattga
     5941 gtgaaagtcc tttcttctga aattcaacaa atttcttgaa acgcaaaact tctaacatac
     6001 caaatataaa gaaaaaatag gtagccagct ggatcaatcc ataaggcact tccccgccca
     6061 ggagtttcag caaacgattg ggaaaaaatg aatagaatat agtcaggata agtcccagcg
     6121 ctgtagtaag gatgattgtc agaatgaagt tggtaaatct tgtattcatt tcaattatat
     6181 caaattttca aacaagtaaa tcaattggga ttccaaccaa tatcatcacc ggaaaccgtt
     6241 aatgtgccta gtttggtgct cccactgttg tcttcaaaag gaggtttggc aacatttatc
     6301 ttccggctta atatattgaa tctcttgacc ggtccatcat ttattttcat cgaaataaaa
     6361 cctgaaacct ccacgctacc tgtcttagcg gaggccgttt gagcatatat ttcataagtt
     6421 cccggtacga ggttatcatt ttgaacaatg gcttcatagg gaaggtcttc gtaatttgcc
     6481 aagttgtcat cccaggttcc gaaaccggcc tttctatggt tattctgata aaaaacccaa
     6541 ttgcttgtac ctgctgaccg catataaagg tggaccttgt catccttatc atcccaattg
     6601 atcaaaatta tcaaaggagg ctctttgccc atggccgcat cacccctgct aagtgaagcg
     6661 tcgggtggag ttatccccgg ggatgactgt ttgccgttgg cgactaaaga aacatcagtg
     6721 ggacccggac tgagagcagt ttgagcagcc tttaactctg cctgaatcct ttttaattct
     6781 tcgtcatccg gattacccgc aagctgttgc tgtatggttc tgattgtctg catagcacga
     6841 tcgtacttct gtgcgacatg gctatattga attgttttaa cattcagggt attctgtagt
     6901 gttctgattt cagattttaa attttctatg gaagattgaa gggacacaga acttgcgacc
     6961 agatcgcgat attccttttc aggtacaatg gactcaagct tagcaaccat actgtccata
     7021 tgtgcctttt tgacatccag tgagtccagt gcttttaatt ttttcaaatc aaaaaaagac
     7081 attttgggaa ctataataaa caaaataatc gttgcgccca aggctccagc caacatgtca
     7141 ataaaggaaa tattgacaag atgaaattct ttcgctctac gcataatatt ctattaaaat
     7201 gaatactcag ataattttta tcgatgtatt ccctatagtt ataatatcgt tttggtatag
     7261 ttgaaatcct tcttcatcat ctatcttcaa gccattgaca taggtgccat tttttgaaaa
     7321 tttccagtta cctccatctt ctatccattg tccatctctt agatataccg cggatccaat
     7381 ggtttcgaag gtggcgtgtc gccgggatat atagtttgaa atggtttcca taatctcaat
     7441 ttcattatca ttgaaggaag ttgctctacc catacgtgca aatccatcca gccggatatg
     7501 atattgtctg tcatattctt caccatccaa tatctgcaag gtaaatgagg acttgacagg
     7561 ttgcttttca tccg
//
LOCUS       DAOQMC010000111         7544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3966_length_7544_cov_4.530111, whole genome shotgun sequence.
ACCESSION   DAOQMC010000111 DAOQMC010000000
VERSION     DAOQMC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3966_length_7544_cov_4.530111"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKV31_05700"
     CDS             <1..198
                     /locus_tag="PKV31_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92381.1"
                     /translation="DKWNFFQTDNFNNNSQPLYALITNTGKLLNTPVGYTPDPEQYAT
                     FLQCGIDAFKASEGKETIGSR"
     gene            complement(195..1073)
                     /locus_tag="PKV31_05705"
     CDS             complement(195..1073)
                     /locus_tag="PKV31_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92382.1"
                     /translation="MEFLRRKDIDTDRWDAVIDSSATGLPYAYSAVLDECCNGKWDAI
                     VGDAYNYVLPLPFNRKLFGFKQYYQPYLLQQLGVFGNMPASVEMDAIMHMIKSHSIRS
                     MIAFQEENTQLLAGAGGNLKTNFVLDLLPGYDYLSSQYKKNLKQRIGRSFDDEFTGSE
                     GLSMDDAIDMYFQWTYAKYNKSEKLSKRYIHNWVRIFNHLGYAHVVALRAQDATIHSG
                     IIYLKTHSRIILSLQFSHPDYKQYSGPSRLIDYLIRQHAGSRMRLDFEGSQIPSIAEF
                     YGSFTPQNMPYTVIKS"
     gene            complement(1075..1710)
                     /locus_tag="PKV31_05710"
     CDS             complement(1075..1710)
                     /locus_tag="PKV31_05710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNB92383.1"
                     /translation="MDIVSKAIEDYCESFGDNPPGLLLDLDTYTHEHHHAPNMLSGSF
                     QGRVLAMISRVVRPKTVLEVGTYTGYSALCLAEGLADGGVVHTIDIDERLRQTHELFI
                     GQSRYADRIKVYFGDAKILIPGLGLRPDLVFIDGAKKDYSAIFDMVLPMMNSGGVILA
                     DNVLWKGKVLDEEMDDRTAAIHEFNRKVSQCELVYKVLLPVRDGLFWITKK"
     gene            complement(2440..2805)
                     /locus_tag="PKV31_05715"
     CDS             complement(2440..2805)
                     /locus_tag="PKV31_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNB92384.1"
                     /translation="MKTDITNKQDILKIILIFYDRVKTDDVIGIFFTEVVQINWDKHI
                     PHMVSFWESVLFYTGDYSGSPINTHRGIHQKYKTQPKHFARWIKLFNQVIDENYSGPN
                     AEKMKNHTISIAKVMQQKI"
     gene            complement(2848..3615)
                     /locus_tag="PKV31_05720"
     CDS             complement(2848..3615)
                     /locus_tag="PKV31_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB92385.1"
                     /translation="MFKVLKYVALDIFRNKIIIAYTLMLALFSWSAFALEDNVAKGLL
                     TELNIILLTVPLFSVLFSTIYVYNSSEFIELLVSHPVKRNVIWTALFVGLSLSMVGAF
                     IISTGIPIIIFADFATGLMMIVAGSVLSVIFVAIAFLSSIMARDKAKGIGISIILWLY
                     FALLFDGLVLFLFLQFSDYPIEKFVVMLSALSPIDLCRILVLLRLDVSAMMGMTGAVF
                     KDFFGTGGGLAVSFLLLLMWIAVPYGISLRKFKHRDL"
     gene            complement(3608..4321)
                     /locus_tag="PKV31_05725"
     CDS             complement(3608..4321)
                     /locus_tag="PKV31_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB92386.1"
                     /translation="MIEIKNVSKKFGKLQALDKLNLTLQQGECVALIGPNGCGKTTLI
                     KSILGMVLPDEGEIIFNGKSIKHDINYRNEIGYMPQIGRYPENMTIGQVLELIKGIRD
                     GHQETDKELYEQFLIATMLEKKMRTLSGGTTQKVSAVLAFLFRPKVLILDEPTAGLDP
                     IAALILKDKILKAKEQGCLILITSHLLNELEDIVSEILFMQEGKLILHDSVDRLKELT
                     GEDSIARSIVHLLKENGHV"
     gene            complement(4318..5577)
                     /locus_tag="PKV31_05730"
     CDS             complement(4318..5577)
                     /locus_tag="PKV31_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNB92387.1"
                     /translation="MSGFYNKAAINILLFVLLVLVSDSVSGSVLEVGKGKRYATINDA
                     LKQARDHDTVVIFSGLYKEGNIRITRPLSIIGRDWPVLDGLKKTEIITVRASNVNISG
                     LVLKDCAQGSMTDPAAIRVAEASNVNIIGNRLQNNFFGIYLQYAKHCLIKDNQIFASQ
                     KHEYQSGNGIHCWKSDSLQVIGNKIAGHRDGIYFEFVTNSIIWRNIAENNLRYGLHFM
                     FSNDDAYITNFFRNNGAGVAVMFTKRVTMINNTFTENWGDAAYGLLLKEITDCYMSGN
                     KFFKNTTGIFLDGSNRITIEKNSFNGNGWGMRMQANCMDNYIARNNFMGNTFDVATNG
                     TLTLNKFEENYWDNYDGYDLNKDKIGDVPYRPLSLYSVLVEKNPPAMLLYRSFMITLL
                     DRSERIIPSLTPENFIDNAPKTVPYQI"
     gene            complement(5599..6042)
                     /locus_tag="PKV31_05735"
     CDS             complement(5599..6042)
                     /locus_tag="PKV31_05735"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB92388.1"
                     /translation="MKNILFVIICLAFVTGFNSCSTDGPEPILLNKDMCDNCRMNISD
                     ARFGAEVQTQKGRYYKFDDLKCLAHYAVEQPNGPIKSFYIHDFSQNNVLIEATKAYYV
                     WSKEFSSPMRGNTAAFANRADAEKVAQQYGSAVDDWETAKKRILE"
     gene            complement(6039..6617)
                     /locus_tag="PKV31_05740"
     CDS             complement(6039..6617)
                     /locus_tag="PKV31_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92389.1"
                     /translation="MKNRNSNFSRVILFVSSLLLVCVLFVPLWRIDLDAPQYPEGLRL
                     LLYPHKLGGDVDIINGLNHYIGMKTLHTEDFIEFKVLPYLIGFFALLFLVSGIINTRK
                     WLWISVISFVIFGIVSMIDFYRWEYDYGHNLDPNAAIKVPGMAYQPPLIGFKQLLNFG
                     AYSIPSTGGWLFVGAGVLLVLALVKEKYMKKS"
     gene            complement(6977..>7544)
                     /locus_tag="PKV31_05745"
     CDS             complement(6977..>7544)
                     /locus_tag="PKV31_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HNB92390.1"
                     /translation="GPELSQSAQLYDISGDKMELILDYPTIGEPHYAQAIPGELIKDK
                     QVKIFDIKANKHKYAAHGEAETKVVREGNQVHVYITAIRSHFAPDCIEGVKVGDEVYF
                     HVTNLEQDWDVPHGFGVKGANNAELLIMPGETATLKWTADRVGIFPIYCTDFCSALHQ
                     EMQGYVRVSPKGSNTPLKFWLNNKADVQ"
BASE COUNT         2297 a         1647 c         1446 g         2154 t
ORIGIN      
        1 gataagtgga attttttcca gaccgataat ttcaacaaca acagccaacc gctgtacgcg
       61 ctgattacaa acacaggtaa gcttttaaac acaccggtag gctatacacc tgatcctgaa
      121 cagtacgcaa ctttccttca gtgtggaatt gatgcattca aagcttccga aggaaaagaa
      181 acaattggta gtcgctaaga cttaatcact gtatagggca tattctgagg tgtgaaagaa
      241 ccatagaatt cggcaatgga tgggatttga gagccttcaa aatccaatct catcctactg
      301 cctgcatgtt gccggatcag ataatctatc aatctggagg ggcctgaata ttgtttgtaa
      361 tccggatgac tgaactgaag ggatagaatg attctggagt gtgtcttcag gtagatgata
      421 cccgaatgaa ttgtggcatc ctgcgctctc agagctacta catgtgcata accaagatgg
      481 ttgaagatcc ttacccaatt atggatgtat cgctttgata gtttttcgga tttattgtat
      541 ttggcatatg tccattggaa atacatatcg atagcatcat ccatggaaag cccttcactt
      601 cctgtgaatt catcatcgaa cgaccttccg atcctttgtt taagattctt tttgtattgg
      661 cttgaaaggt aatcatagcc cggaagaaga tcgagaacaa agttggtctt taaattcccg
      721 ccggcacctg caagaagttg tgtattctct tcctgaaagg caatcatact tcggatgctg
      781 tgactcttga tcatatgcat aatggcatcc atctcgacgc tcgccggcat attgccaaag
      841 acacccagtt gctgcagcaa gtacggctga tagtattgtt tgaagccgaa taatttcctg
      901 ttgaagggca agggaagtac atagttgtat gcatcaccga ctatggcatc ccatttccca
      961 ttgcaacatt cgtccaaaac tgctgagtat gcatatggca atccggttgc cgagctatcg
     1021 atcaccgcat cccatcggtc tgtatcaata tcctttcttc gcagaaattc catcttattt
     1081 cttagtaatc cagaaaaggc catcacgcac cggtagcagg actttatata caagttcgca
     1141 ttgacttacc ttcctgttaa attcgtggat cgcagctgtc ctgtcatcca tttcttcatc
     1201 cagtactttt cctttccaaa ggacattgtc ggccagaatc actcctccgg aattcatcat
     1261 aggaagtacc atatcgaaaa tagctgaata gtcttttttt gccccatcaa taaagaccag
     1321 atccggcctt agaccaagcc cgggaataag tatcttagca tcgccaaaat acacttttat
     1381 cctgtcagca taccggcttt ggcctatgaa gagttcatgt gtctgcctga gacgttcatc
     1441 aatatcgatg gtatgtacta cacctccatc cgcaagacct tcagcaaggc ataatgcaga
     1501 atagccagta taggtaccta cttcgagcac ggtcttaggc ctcacaacac ggcttatcat
     1561 tgccagcacc cgaccctgaa agctacctga caacatattg ggtgcatgat ggtgctcgtg
     1621 ggtgtaagtg tccagatcta acaagagccc gggtgggtta tctccaaatg actcacaata
     1681 atcttcgatt gctttagaaa cgatgtccat aaatattttc ctaaaagaaa aaaaacagtg
     1741 tagtctggct tataaatgct gacccgatca gggtatatca ataaaccaga catgcattca
     1801 atcccaggca aaaataatca aaataagggg atgaggtcta agtacatcca ggtattgtgt
     1861 tcattacaaa tcgtccttta cttttattaa gaaaaaagca gggaaatata aaccatagac
     1921 ttagcacaaa aggatattaa cttgttataa ataaattgat tatatattac aaattctttt
     1981 tctttccaca agattaccct taaatccatg ccgtgaaaaa atcatgacga ttctagtgta
     2041 atgtatagct taaataactt aatattcgaa atgccagctt tgtttttatc gactttgccg
     2101 attgttgaca aaggcgtatg ttaagtttgt gatatattat accaagatga tttttcagct
     2161 gtgctatttc ttccatacag taggtcaatc gttataaccc caccccactt ttttataaaa
     2221 acggggtcac tttccaccta tcaaagaaaa ataaaaatct agcctgttgt tttgtgggaa
     2281 aaagccggaa gaatacaagg aaatcaatca cagtcatcag ttggaaagat gacatccaaa
     2341 gtcaagtcaa cccatgcatt acctcagatg gtggctgaaa acagtgtgaa agtcacaaat
     2401 cgaattgcgc aggctgaaaa tagctatttt atctaaaact cagatctttt gttgcataac
     2461 tttagcaatc gaaatggtat ggtttttcat tttctctgca ttcggcccgg aataattttc
     2521 atcaatgacc tgattgaata atttaatcca tcgggcgaag tgcttaggtt gggttttata
     2581 cttttgatgt ataccccgat gtgtattgat agggcttccc gaataatctc ctgtataaaa
     2641 gaggacactc tcccagaagg aaaccatatg gggaatgtgc ttatcccaat tgatctgaac
     2701 aacctcagtg aaaaaaatgc caatgacatc atcggttttg acacggtcat agaaaatcag
     2761 aataatcttt aagatatcct gtttatttgt aatatccgtt ttcatttttt ctaattagaa
     2821 ataaaatatc aaacattaat atccatatca aagatctcta tgcttaaatt ttcgcaatga
     2881 aataccatac ggtacagcaa tccacatcaa cagtaacaga aaagacacag ccaagccccc
     2941 tcctgttccg aaaaagtcct tgaaaacagc acctgtcata cccatcatgg ccgatacgtc
     3001 caatctcaat aaaaccaata tcctgcaaag gtcaatcggg ctgagcgcac tcagcattac
     3061 cacaaatttt tcaattggat aatctgaaaa ttggaggaac aggaacaaaa ccaagccatc
     3121 gaatagcaaa gcgaaataga gccacaatat gatagagatg cctatcccct tggctttatc
     3181 ccttgccatg atgctactga gaaaagctat tgcgacgaaa atcactgaca atacggaacc
     3241 ggcaactatc atcatcaaac cggttgcaaa gtcagcaaaa attattatcg gtatgccggt
     3301 gctgatgata aacgctccga ccatactgag agaaaggccc acaaaaaggg ccgtccagat
     3361 aacattcctt ttaacgggat gactaacaag caattctata aattcgctgc tgttatacac
     3421 gtaaatggtg gaaaaaagga ccgaaaacaa cggcactgtc agcagaatga tgttcaattc
     3481 agtaagtaat ccttttgcta cattgtcttc aagagcgaat gcactccagg agaataatgc
     3541 aagcatcaag gtataagcaa tgattatctt gttgcgaaaa atatccaatg caacatattt
     3601 caatacttta aacatggcca ttctctttta gcaggtgaac gatggatctt gcgatggaat
     3661 cttcaccggt aagttccttc aaccggtcaa cgctgtcatg cagaatcagc ttaccttcct
     3721 gcataaacaa aatttcggaa acaatatcct ccaattcgtt gagcaaatgg gaagtgatca
     3781 ggatcaggca cccttgttcc tttgctttga gtatcttgtc cttgaggatg agtgctgcga
     3841 tgggatcaag gcctgctgta ggttcatcaa gtatgagcac cttgggacgg aaaaggaatg
     3901 ccagaactgc acttactttt tgggttgttc ctccactgag tgtcctcatc ttcttctcga
     3961 gcatggtggc aatcaaaaat tgctcataca actctttatc ggtttcctga tggccatcgc
     4021 ggatgccttt aatcaactcc agtacctggc cgatggtcat attctcaggg tagcggccaa
     4081 tctgaggcat ataaccgatt tcattccggt aattgatgtc atgcttaatg gattttccgt
     4141 tgaaaataat ctcaccttcg tccggcaaaa ccatacccag aattgatttg atcagagtgg
     4201 tcttaccaca gccattcggg ccaatcagag caacgcattc tccctgctgc aaggtcagat
     4261 taagcttatc caaggcctgg agtttgccga actttttact gacattctta atttctatca
     4321 tatctggtaa ggaaccgttt ttggtgcgtt atcgatgaag ttctcggggg tcaagcttgg
     4381 aattatccgc tcagaccgat cgagcaacgt aatcataaag ctccggtaca acaacatagc
     4441 gggtggattc ttttctacga gaacggaata caggcttaat ggtctgtaag gcacatcgcc
     4501 tattttatct ttgttcaggt catagccatc ataattatcc cagtaatttt cctcaaattt
     4561 gttaagagtc aaggtaccgt tggtggcaac atcaaaagta ttgcccataa aattattgcg
     4621 ggctatataa ttgtccatac aattggcctg catcctcata ccccaaccgt tgccattaaa
     4681 actgtttttt tcaatcgtta tgcgattcga cccatccagg aagatgcccg ttgtgttttt
     4741 aaaaaacttg tttccggaca tataacagtc ggtaatctct ttgaggagca agccataagc
     4801 ggcgtcaccc caattttccg tgaaagtatt atttatcatg gttacccttt tggtaaacat
     4861 gacggccact cctgctccat tattcctgaa gaaattcgta atataagcat catcatttga
     4921 aaacataaaa tgaagcccat atctgagatt gttttcagca atatttctcc agataattga
     4981 attggtaacg aattcaaagt agataccatc gcgatgtccg gcaatcttat taccaattac
     5041 ttgcaggcta tcacttttcc agcaatggat gccattgcct gactggtact cgtgtttttg
     5101 agaggcaaat atttgattat ccttgattaa gcaatgtttg gcgtattgaa gataaatgcc
     5161 aaaaaaatta ttttgaagtc tattgccaat gatattaacg ttgcttgctt cagcaaccct
     5221 gattgcagcg ggatcggtca tagagccctg agcgcaatct ttcagaacca atccggaaat
     5281 attgacattg gaagctctga cagtaataat ttcggtcttt ttgaggccat cgagaacagg
     5341 ccagtcacga ccgatgatgg aaagagggcg cgtaattcta atatttccct ccttgtacaa
     5401 tccagaaaaa attaccacag tatcatgatc ccgagcctgc ttcagcgcat cgttgattgt
     5461 ggcatacctt ttacctttgc ctacttccag aacagagccg gacaccgaat ctgaaaccaa
     5521 caccaatagg acaaaaagca aaatatttat tgctgcctta ttataaaagc cggacattca
     5581 aaaaatagtt aataacaatt actccaggat ccgcttctta gcggtttccc aatcatccac
     5641 agcacttcca tattgctgag ctaccttttc agcatcggct ctattggcaa aagctgctgt
     5701 gttgcctctc atggggcttg aaaactcttt gctccaaacg taatacgcct tggtggcttc
     5761 aattaaaaca ttattttgtg aaaaatcgtg aatatagaac gattttatag gaccgttagg
     5821 ttgctcgact gcataatgtg ccaagcattt caggtcatca aatttgtaat atcttccttt
     5881 ctgagtctga acttccgctc cgaatcttgc gtcggaaata ttcatacgac aattgtcgca
     5941 catatcctta ttaagaagaa taggctcagg gccatcggta ctgcatgaat tgaacccagt
     6001 aacaaaagcc aggcaaatga ttacgaataa tatatttttc atgatttttt catatacttt
     6061 tccttaacaa gagcaagtac gagtaaaact cctgctccaa caaacaacca acctccggtg
     6121 gaaggaatgg aatatgctcc gaagttgagc aattgcttaa accctatcaa aggtggttgg
     6181 taggccattc ccggcacctt gatagccgca tttggatcca gattgtggcc gtaatcatat
     6241 tcccatctat aaaaatcaat catcgaaact ataccgaaga ttacgaagct tatcaccgaa
     6301 atccacaacc acttccttgt attaatgatt ccagagacca gaaacaacaa ggcaaaaaag
     6361 ccaatcaaat aaggcaaaac cttgaattca ataaagtcct cggtatgaag ggttttcatg
     6421 cctatatagt gattaagacc attgatgata tctacatcac ctccgagttt gtgtggatat
     6481 aaaagcaatc tcaagccttc cggatactgc ggagcatcca gatcaattct ccatagagga
     6541 acgaacaaaa cgcaaacgag tagtaaggaa gaaacaaaaa gaattactct ggagaaattg
     6601 gaatttctat ttttcatagt gagtataagt tggttaaaag ataaatacat ggcgaagggc
     6661 tattctactt ccttgaggcg gccccgttca ggattccata aggatatatc accacccaca
     6721 tgtcctccat aaaaacaggt ttcagaacct aattaaaatc atgaagcccg aattctcagg
     6781 gaaaaatata atcatcgctc agtcaatctt tattggctta agtgtgaaaa taccgaccta
     6841 cctacaaagt agaagtattt tcgccaggaa gatagaaata ttcaaaatca gaggagactc
     6901 aagcttcaaa ggtaaagggg caaatcatca tttgcggacg aaatgcccct tatgaaattt
     6961 atttagaaat gaaaatttat tggacgtccg ctttgttgtt caaccagaac ttcaaaggtg
     7021 tattggatcc tttaggagaa accctaacgt acccttgcat ctcttggtgc aaagctgaac
     7081 aaaagtctgt acaatagatt gggaatatac ccactctgtc agctgtccac ttcaaggtag
     7141 ctgtttcgcc cggcataatc aagagttctg cgttgttagc tcccttcact ccgaaaccat
     7201 gcggtacatc ccagtcttgt tcaaggttgg ttacatggaa gtacacttca tcgccaactt
     7261 tcacgccttc aatacagtca ggtgcgaagt gtgacctaat agctgtaatg tatacatgta
     7321 cctgattacc ttcacgaacc acctttgttt cagcttctcc atgagcagca tatttgtgct
     7381 tattagcttt tatgtcaaag attttaactt gtttgtcctt gataagttcc ccgggtatgg
     7441 cctgtgcgta gtgaggctct ccgatggttg ggtagtcaag aatcagctcc attttgtcgc
     7501 cactgatatc gtacaactgg gcactttgtg acaactcagg gcct
//
LOCUS       DAOQMC010000112         7526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3980_length_7526_cov_4.422969, whole genome shotgun sequence.
ACCESSION   DAOQMC010000112 DAOQMC010000000
VERSION     DAOQMC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3980_length_7526_cov_4.422969"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..881
                     /locus_tag="PKV31_05750"
     CDS             300..881
                     /locus_tag="PKV31_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB92391.1"
                     /translation="MQLQSICDQELIQLYLNGNERAFEELLHRHKSKIYTTIYMFVKD
                     EHQAEDIFQEVFIKIIDTLRRGKYNHEGKFVQWAVRIAYNLCVDNFRRSKRRSIFNST
                     ENFDIFDVLSVAEDSHEQAIIKSQSHERLRKLIDDLPPEQREVIILRHYADMSFKEIS
                     QVTRVSINTALGRMRYALINIRRNLTEKEALAI"
     gene            967..1635
                     /locus_tag="PKV31_05755"
     CDS             967..1635
                     /locus_tag="PKV31_05755"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNB92392.1"
                     /translation="MYSKTTLIDDYRHKGLRKKLLQELRSKGITSDTVLTAFDKVPRH
                     FFLDKAFEELAYQDRPVPIGYRQTISQPYTVALQSQLLNVQKRQKILEIGTGSGFQAC
                     ILAEMGARVYTIERQEGLYRKTMSKLEQIGYSHIKCFLNDGFKGLPYHAPFDKIIVTA
                     APGELPYDLIDQLVIGGVMVLPKGNHKNQKMMLLTKTSHNTYTLEEKVSAYFVPMLEG
                     IEQL"
     gene            2026..3336
                     /locus_tag="PKV31_05760"
     CDS             2026..3336
                     /locus_tag="PKV31_05760"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNB92393.1"
                     /translation="MTDNIKAQLKSNTFYIIKMALKFKSLKQIGYIFFLIIPSLLLLS
                     CSKETQVVQEPTRDIDIKIPHIHIKIGDSSEIDSKDYKAAQIFIDSKGGFKDIQVSGK
                     VAGRGNYSWSLPKKPYKIKFDNAQSIFGLPAYKKWVLLAEYMDGSMLYNSIPFGTAYL
                     LKLPYSNHIFPVDVTINGQYRGLYILTESLEVGKGRIDIGEDGTLLELDSYYDEKWKF
                     ISGKFKLPVMVKYPEEEDMTEEKLTEIKGDFEALEKLIYATDFPNNNYLDLFHDTAFV
                     DYMIVYQLSANFEINHPKSTYMNKKSNGKYKMGVIWDFDWAMGYNDITGIHYDPAYAT
                     SPLIYENGNNPGTKFFGRIMQDPKIQKLFLHRWKWFRQHKYQSLRKFVSDYSLLINES
                     YKEDHNLWGLRGSTGVLASDLQRALSWLDARADYIDDYADSFNQ"
     gene            complement(3359..3949)
                     /locus_tag="PKV31_05765"
     CDS             complement(3359..3949)
                     /locus_tag="PKV31_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92394.1"
                     /translation="MIRIERYIGSVIIGLVFSILLFGVFVVGMSSCGTSGARLRPFTE
                     RFYKDSGLTYDQLKHVQFYIDRDIILYRMIGSNQSTVDQGRIATRGGRKVEEIVIRSG
                     TPGTLVFMPKENRLGISFDADNAGNYLMFGPNPKNNNRYCLLAIEWDRDEGMVSYGPN
                     HWKTPASSAFATLMVNHRNMKKTKYSSQSPEGRVVH"
     gene            complement(4054..4506)
                     /locus_tag="PKV31_05770"
     CDS             complement(4054..4506)
                     /locus_tag="PKV31_05770"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNB92395.1"
                     /translation="MEDLYTDHYFMKMALKEAQKAYEANEVPIGAVIVARNQVIARAY
                     NQVQMLNDVTAHAEILAITAAEQAYGSKYLNDCTLYVTVEPCPMCAGAIKWSQLDRLV
                     YGTDDEKYGFMKFGKSILHPATKVAYGVCREEAMELMQQFFAERRKHK"
     gene            complement(4630..5913)
                     /locus_tag="PKV31_05775"
     CDS             complement(4630..5913)
                     /locus_tag="PKV31_05775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB92396.1"
                     /translation="MIVVCFGPGPVFKGGIANYNTSLAKAFDKRTDCEVHIVSWTQQY
                     PAIIPRDFIDKASRVDQLAGTGISVHYLTNYNNPFTWTQTVEKIRSLKPDLVIFQWAI
                     AIQGLPISFIAKRLEKSGIKTVFDLHFVIQKENSSIDRIFTKWALSRSKRFVTHARKT
                     FDELKQLFPDKNFTFIDRGDSIDDAILSNGTESLVINLYHPVYDMFTRRSDFDVDAFK
                     AEHRLRKHVFLFFGFIRKYKGLHNVIPAFRILADRRDDVSLIICGESFWKTLDKSRMS
                     TKVKSALFGLAKKIFLRKSDDESDYNPLALVDELNLKDRVVMFNSFVPNEEVHRYFQV
                     SDAVLLFYEYATPSGVESIGYNFNMPILATAVGHFPETVVDGYNGYLVQPDNIEDMAA
                     TMEKFLEHPIDRENVAATSKDMSWDNYAARIARLG"
     gene            complement(5914..6795)
                     /gene="rfbD"
                     /locus_tag="PKV31_05780"
     CDS             complement(5914..6795)
                     /gene="rfbD"
                     /locus_tag="PKV31_05780"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNB92397.1"
                     /translation="MATIGITGAAGQLGQEFAFLSQYFPRQEFVFFDSEELDITDVDQ
                     LQDELEDYDIDFLINCAAYTKVDQAEQEIEKAYKINRDGVSYLADYCEEKNITLIHFS
                     SDYVYHNGLDRPLREDDATEPKGVYAASKLAGEQEVFKRCPNSLVFRTSWIYSSFGTN
                     FVKNILNASSSKSEFNLVFDQIGCPTYARDLADRVLKIVEGLAVGEYDIRDCAGIYNI
                     TNSGVTSWYDLTKQVFNLSGKQCSVSPILSSDYPSAVTRPPYSVLDQSKLKASFGIVM
                     PYWMDSLAKCLKALNDF"
     gene            complement(6824..7372)
                     /gene="rfbC"
                     /locus_tag="PKV31_05785"
     CDS             complement(6824..7372)
                     /gene="rfbC"
                     /locus_tag="PKV31_05785"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNB92398.1"
                     /translation="MPISTVDIEGILIFTPRIFTDDRGYFYESFRADQYADFGIIKPF
                     VQENQAYSTRGALRGLHYQTGEMAQAKLVRVIVGEVLDVAVDLRPTSKTYGKVYSIIL
                     SGDNHKQMYVPRGFAHGYLVLSDEAIFTYKCDNYYSKQHEGGIRYDDPLLNIDWQMED
                     AELILSEKDLELPYFGNHMPAI"
     gene            complement(7378..7473)
                     /locus_tag="PKV31_05790"
     CDS             complement(7378..7473)
                     /locus_tag="PKV31_05790"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92399.1"
                     /translation="MKESYKLFLENQIRKHFDFTGVKISIVFKEK"
BASE COUNT         2283 a         1654 c         1467 g         2122 t
ORIGIN      
        1 agaaagattt accaaaatgt taaagaaagg gcaaaggata ccggtttgtc tttaaaaata
       61 aaaaaaaaca catttaatcc atgtttagaa cctgtgtaaa acactgactt ctgaagatcc
      121 aaagcaattt atcttaagtt aaatttgtgt taaggagagt atgagtaaaa aataaaatat
      181 atctttgaac tgaaattatt attcaaaaga catttttagt ttaaaaatat ttaaccagcc
      241 tatacgcata gtttttactt ttcaacaatc cattatccta agtaaaaaaa gtagaaatta
      301 tgcaattaca atcaatctgc gatcaggaac taatccaact gtatctcaac ggaaatgagc
      361 gtgcatttga agaattgctt cacaggcaca agagcaagat ttatactact atctacatgt
      421 ttgtcaaaga tgagcatcag gccgaagata tctttcagga agtcttcata aagattattg
      481 ataccttgcg tagagggaaa tataatcatg aaggtaaatt tgtacaatgg gccgttcgca
      541 tcgcttacaa cctttgtgtg gataatttca gaagatcaaa aagacgctct atcttcaact
      601 caaccgaaaa tttcgatata ttcgatgttt tgtcggtagc tgaagatagc catgaacagg
      661 ccatcatcaa gagtcaaagt catgaaagac tccgcaagct tattgacgat ctgcctccgg
      721 agcaaagaga agtcatcatc ctgcgccatt acgcagacat gagtttcaag gaaataagcc
      781 aggtgacaag ggtaagcatc aataccgctt taggcaggat gcgttatgcc ctgatcaaca
      841 tccgtcgcaa tctgaccgag aaagaggccc tggcgatcta agtcaatacc tttatttcaa
      901 aaaaaagcta atttatttta caataagtta gcttttttca ttttacatat gtaactttgc
      961 gttaatatgt attcgaaaac tacacttatc gacgattaca ggcataaagg cctcagaaaa
     1021 aaattgctgc aggagctcag aagtaaaggc ataaccagcg ataccgtcct aaccgccttc
     1081 gacaaggtgc ccagacattt ctttctggac aaagcttttg aagaactggc gtaccaggac
     1141 cggcctgtcc ctatcggata tcgccagacc atttctcaac catacacggt agcccttcag
     1201 agtcaattgc tcaatgttca gaaaaggcag aaaatccttg aaatcggaac cggatccggc
     1261 tttcaggcct gcattctggc agaaatgggt gcccgtgtat atacaatcga acggcaggaa
     1321 ggcctttatc gtaagacaat gagcaaactg gaacaaatag gatactccca tattaaatgt
     1381 tttctcaacg atggattcaa ggggctgcca taccatgctc cctttgacaa aatcattgta
     1441 acggcagctc cgggagagtt gccctatgat ttgatagatc aattggtcat tggtggtgtg
     1501 atggtattgc caaaaggaaa tcacaagaat caaaaaatga tgttgctaac caaaacatca
     1561 cacaatacct atacgcttga ggaaaaggtg agtgcttact tcgtccctat gctcgaagga
     1621 attgaacaac tatagggatt tgtacaagag gacaggcgat gatatttcca tgttggatca
     1681 tcaagacagg agcgctataa taggtcaaga gaacattcac aattgaatta ccgacaaatt
     1741 tacagcaggt atgatctttt ctgtgtccat aaatcctatg ataaatttct tgtccaatcg
     1801 tacaactttt cgtacacgct caatcattgg gcaggactga caacctccca aaccattacg
     1861 tctaaccgga caaccatgac gagaggatta ggcgaatgcc cgtgatgcca ggtacaatta
     1921 tattcatcta aatgatttat ttacccaagc cgccatcaac ccggactatt atattacctg
     1981 aaaagatagt tatttcttac atttgagggg taataacggg ctaagttgac agataacata
     2041 aaagctcagc tcaagtcaaa cacattctat attattaaga tggcgcttaa gtttaagtct
     2101 ttgaaacaaa tcggttatat tttttttttg atcattcctt cgctcttact tttgtcatgt
     2161 agtaaggaaa cacaggtagt acaggaaccg acccgcgata tagacataaa gataccacat
     2221 attcatatta aaattggtga cagttcagaa atagattcca aagattacaa agcagcgcaa
     2281 atattcatcg acagcaaagg tggattcaag gatattcagg taagtggcaa agtcgccgga
     2341 agaggcaatt acagttggtc gctcccgaag aagccctata aaataaaatt tgacaacgct
     2401 caatccattt tcggccttcc cgcttataaa aagtgggtcc tgctggctga atatatggac
     2461 ggttccatgt tatacaactc cattccattt ggtactgcct accttttgaa acttccatat
     2521 tccaatcata tttttcctgt cgatgtcacc atcaatggcc aatatcgcgg tctgtacata
     2581 ctgaccgaaa gtctggaggt cggcaagggc cgcattgata tcggagagga cggaacctta
     2641 ctcgagctgg attcgtatta tgatgaaaaa tggaaattca tttcgggcaa attcaaactt
     2701 cccgtcatgg ttaagtatcc cgaagaagag gatatgacgg aagaaaagct gaccgaaatc
     2761 aagggcgatt tcgaagcgct tgaaaaactg atctatgcaa cggactttcc aaacaacaat
     2821 tatctggatt tgttccatga cacggctttt gttgattata tgattgtgta ccagctttct
     2881 gccaatttcg agatcaacca ccccaagagt acatacatga acaaaaaatc caatggcaaa
     2941 tacaaaatgg gcgttatctg ggattttgac tgggcaatgg ggtacaatga tattacagga
     3001 atccattacg atccggctta tgctacctct ccattgatat atgaaaatgg aaacaaccca
     3061 ggaactaaat tctttgggcg aatcatgcag gatcccaaga tccaaaaact attccttcac
     3121 aggtggaaat ggttcaggca gcacaaatac cagtccctca gaaaatttgt gtccgattat
     3181 tccctgctaa tcaacgagtc ctataaggaa gatcacaatc tttggggatt aaggggttct
     3241 acaggtgtct tagcttcgga cctccaacgt gctttaagct ggctggatgc aagagcagac
     3301 tatattgatg actatgcgga ttctttcaat caataggaag aatatagtgt tgaatacttt
     3361 aatgtaccac cctaccttca gggctctgtg aggagtattt cgttttcttc atgttccggt
     3421 gattgaccat caaagtggca aaggcactcg aagccggagt tttccaatga ttcggcccgt
     3481 acgacaccat tccttcatcc ctatcccact caatggcaag cagacaatag cgattgttat
     3541 tcttgggatt aggaccgaac atcagataat taccggcatt gtccgcatcg aaggaaatgc
     3601 caagtctgtt ctccttgggc atgaatacaa gtgtccccgg tgtaccgctc cttattacta
     3661 tttcctccac tttccgacca ccacgggtgg caatcctgcc ttgatcaacc gtgctttgat
     3721 tgctaccaat catcctgtac agaatgatat cccgatcaat gtaaaactgg acatgcttca
     3781 actgatcata agtgagtccc gagtctttgt aaaatcgctc ggtaaaaggc cttaatcgtg
     3841 cacctgatgt accacaggaa gacataccca ctacgaaaac tccaaataaa agtatcgaaa
     3901 agaccagccc aataatcaca ctacctatgt acctttctat tcttatcata atcacaatat
     3961 catttttacc ttttactgtc aaaagataac gcctggacaa aggtcaaaag gcgtttttta
     4021 acattgttta accatgcttt aaatcaattt taactatttg tgcttacggc gttcagcgaa
     4081 aaattgctgc atcaattcca tggcttcttc cctgcagaca ccatatgcaa ctttagtagc
     4141 cggatggaga atagacttcc cgaatttcat aaatccatat ttttcgtcat ccgtaccata
     4201 gaccaacctg tctaattgtg accatttaat cgctcctgca cacataggac agggttcgac
     4261 agtgacatag agtgtacagt cgttaaggta ttttgagcca taagcctgct ccgcagcagt
     4321 gatggcaagg atctcggcat gcgctgtgac gtcattgagc atctgtacct gattatatgc
     4381 ccgggcaatc acctgattgc gtgccacaat gacagctcca ataggtactt cattggcctc
     4441 ataggccttc tgggcttcct taagagccat cttcataaaa tagtgatcgg tatataaatc
     4501 ttccattgca cccattcaaa atccaaagta ttaataactt cagttatcag aaaaatgtta
     4561 tttcccgtaa gtctgtattt tcaaagtacc tgccttaatc aggtagtaaa tgaacgacaa
     4621 attgttcgct cagcctaatc ttgcaatcct cgctgcataa ttatcccaac tcatatcttt
     4681 gctggtggca gccacatttt cacggtcgat cgggtgttca aggaattttt ccatagtagc
     4741 tgccatatct tcaatattat caggctgcac gagatatcca ttataaccgt caactacagt
     4801 ctccggaaaa tgaccaaccg cagtggccag aatcggcata ttgaagttgt agccaatgga
     4861 ctcaacgccc gatggcgttg catattcata aaacaacaat acagcgtcac tgacctggaa
     4921 atatctatgc acctcctcgt tgggcacaaa agagttgaac attaccacgc ggtcttttaa
     4981 attaagttcg tctaccagag caagcgggtt atagtcgctt tcatcatccg atttcctgag
     5041 aaatatcttt ttggccaggc caaacaatgc ggacttgact ttggttgaca tcctgctttt
     5101 atccagtgtc ttccagaaag attcaccaca gatgatcagt gagacatcat ctctccgatc
     5161 tgctaaaatc ctgaaagccg gtattacgtt gtgcagccct ttatacttcc tgataaatcc
     5221 aaaaaacaaa aagacatgtt ttcgcaaccg gtgctcagcc ttgaatgcat cgacatcaaa
     5281 atcacttcgt cgggtaaaca tatcatatac agggtgataa aggttgatca ccagcgattc
     5341 ggttccattt gacaatattg catcatctat ggagtcaccc ctgtcaataa atgtgaaatt
     5401 tttgtccgga aataattgtt ttagttcatc aaaggtcttt cgtgcatgtg tcacaaatcg
     5461 cttgcttcgc gagagtgccc atttagtgaa tatccgatca atggagctgt tttctttctg
     5521 aatgacaaag tgcaggtcaa aaactgtctt tataccgctc ttctcaagtc gttttgcaat
     5581 aaaggaaatt ggcaagccct gaatcgctat cgcccattga aaaataacaa ggtccggttt
     5641 caatgacctg atcttttcaa ccgtttgtgt ccaggtgaaa ggattgttgt aattggtcag
     5701 gtagtgtaca ctgatgcctg ttccggccag ttgatccacc ctgcttgcct tgtcgataaa
     5761 gtcacgcggg atgatggccg gatactgctg tgtccaggat acgatgtgta cctcacaatc
     5821 cgtccgctta tcaaaagcct ttgccaatga tgtgttgtaa ttggctatac ctcccttgaa
     5881 tacaggcccc ggcccaaaac aaactactat catttaaaaa tcatttaacg ccttcagaca
     5941 tttggcaagg ctatccatcc agtaaggcat gacaatcccg aaactggctt tcagcttact
     6001 ttgatcaagt accgaataag gtggcctggt gactgcactt ggataatcgg aggacaagat
     6061 cggactgacc gagcattgct tgccggatag attaaacact tgttttgtca agtcatacca
     6121 tgaagtgaca cccgaattgg ttatattgta aatgcctgcg cagtcgcgga tgtcatattc
     6181 gcccactgca agaccttcaa ctattttaag caccctgtcc gcaagatccc tggcataggt
     6241 aggacagcca atctgatcaa aaacaagatt gaattcactc ttggaggaag atgcattgag
     6301 gatattttta acaaaattgg ttccaaaaga cgagtatatc catgaggtcc tgaaaaccag
     6361 gctattcgga caacgcttga atacctcttg ttcgccggct agtttagagg cagcatatac
     6421 acctttcggc tcagttgcat catcctcccg taatgggcga tccaacccgt tgtgatagac
     6481 ataatccgaa gaaaaatgta tcagcgtgat gtttttttct tcacaataat cagcgagata
     6541 gctcacacca tcccggttga ttttataggc cttttcgatt tcctgttcgg cctgatccac
     6601 cttggtatag gcagcacaat tgatcagaaa gtctatatca taatcttcca attcatcctg
     6661 caattggtca acatctgtta tatcaagttc ttcggaatcg aagaatacaa attcctgtct
     6721 agggaaatat tgcgaaagga atgcgaattc ctgtcccaac tgaccggctg caccggttat
     6781 gccaatggtg gccatagtta aagattgatt gcttttatgt tattcatatt gcgggcatat
     6841 ggtttccaaa atagggtaat tcaagatcct tttcgcttag tatcaattca gcatcttcca
     6901 tttgccaatc tatattgagg agtggatcat cataacggat gccgccctca tgttgtttgc
     6961 tgtaataatt gtcacactta taggtaaata tcgcttcatc cgataaaaca agatatccat
     7021 gggcaaatcc gcgtggtaca tacatttgtt tgtggttatc accactcaaa ataatggaat
     7081 aaaccttccc gtaagtcttg gaagtcggcc taagatccac cgccacatca agtacttcgc
     7141 ccacaatgac cctgactagt ttggcttgtg ccatttcgcc tgtctggtaa tgcaatcctc
     7201 tgagagcgcc tctggtcgaa taagcctgat tctcttgtac aaatggcttg atgatgccaa
     7261 aatctgcata ctggtctgcc cgaaaactct cataaaaata cccccggtca tccgtaaaaa
     7321 ttctgggagt aaaaatgaga attccttcaa tatctacagt actaatgggc atacagctta
     7381 tttttcctta aagacaatgc ttatttttac acctgtaaaa tcaaaatgct tcctaatctg
     7441 attctccaga aatagcttat agctttcctt gatatggtcg ggatgattgc agaaaaatcc
     7501 gaataccgga tgacttgcag acagtt
//
LOCUS       DAOQMC010000113         7523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_3982_length_7523_cov_5.044992, whole genome shotgun sequence.
ACCESSION   DAOQMC010000113 DAOQMC010000000
VERSION     DAOQMC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3982_length_7523_cov_5.044992"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..831)
                     /locus_tag="PKV31_05795"
     CDS             complement(181..831)
                     /locus_tag="PKV31_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNB92400.1"
                     /translation="MKKVFLLVKKDLYSEMRNAHSISSIILYVFATVYIIYSAFINIQ
                     PQNWNVLFWIVFLFVSVNALLRSFDQENSNKALYFYQLAGPLQIFAAKFIFNTILLVL
                     LGLLTLALFSFVAGSPVRDYPLFLLTILLAGIGFASIFSFISAIGAKTGNSHMLTAIL
                     GFPCVVPVLLELLKLSANSLGLLNDTDFTTDILILGSIDIILLSLAFILFPYVWRD"
     gene            954..2183
                     /locus_tag="PKV31_05800"
     CDS             954..2183
                     /locus_tag="PKV31_05800"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB92401.1"
                     /translation="MTSRLNILFFIACLFVSFSAQSQQYVVAELQKGEGGYALLRRYG
                     LDISPCNLAEFYRINNLPYGSALSLGKKYNLPVRIYQYNKSSIRSTVKNFDLVKALEV
                     ERYNKWLKSQQIKSVYFLQDLKLWVPFYIYNCNYDNLAGASQPDNTPDKNLNTTQAEA
                     INKTEIKKNTLDIALFGNKHSQVEVLGNHLRGQVFYIKGGHGGPDPGAMAKIDDKIYC
                     EDEYANDIALRLGRKLIQYGAIVHFIVYDPDDGIRDDEYLPCDKDELHAGKRDIQVNQ
                     IQRLRTRVQIINDYYESYRKKGVKNQKFISIHIDSRNESLELDTHFYYQEGSKVGQEM
                     AVNTQAVFEKKFGELGSRKYTGTVKARDLYVVKYSRPPCLFVEVGNIQNVNDQKRILD
                     PDNRQTLADWLYEGITK"
     gene            complement(2227..5163)
                     /locus_tag="PKV31_05805"
     CDS             complement(2227..5163)
                     /locus_tag="PKV31_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNB92402.1"
                     /translation="MEWNSAEIEEKWKKIWIDSELYKIHTDPDKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFSRYKRLNGFNVLHPMGYDAFGLPAEQFAVQTGVHPAISTQA
                     NINSFRKQLDNLGFSYDWSREVRTCDPSYYKWTQWIFLKMYNAWYDKSEEKSKDISIL
                     IDHFENHGTEGINSSCNSPLQFSASDWKNYSAKEQYDVLMNYRLMYRKISTVNWCEAL
                     GTVLANDEVKDGVSERGGFPVIKKPMMQWSMRTTAFAQRLLDGLDHVDWSDSLKIMQR
                     NWIGRSEGARVFFRLKGRVDNLEIFTTRPDTIFGATFMVLAPEHPLVNELTIQAQQKP
                     MFDYACETAKASEQERLNAQKQMTGVFTGSYAIHPFTGKDIPIWVSNYVLIEYGTGAI
                     MAVPAGDERDRRFAETFNLEIREIIQRNEDDDDIESKNGILINSDFLNGLPVNDAIIK
                     AIETIEKQGFGKRLINYRLRDANYSRQRYWGEPFPIAYDEDGVGHEISPLPLELPDIK
                     DFKPTGDGSSPIARATEWVNATPGLKRETDTMPGFAGSSWYFLRYMDPNNDTLPFSKN
                     AIDYWMDVDLYIGGAEHAVGHLLYSRTWHKFLYDFGLVPTEEPFKRLINQGMIQGVSE
                     KIYLLKESTDKLYFKNNSGQYEVKLLPQSVQVFVSHELIQDYPAKSTSEDKEMEGFSE
                     MSIHIDIVKDYGASTGGYLDKVAMEEFAVWRPEFASAVFVTYGGYYQNGTFHPVTDAD
                     AIRFNTKSEVEKMSKSKYNVINPDTVISEHGTDVFRMYEMFLGPLEQAKPWDTKGIEG
                     VNKFLRRFWNLFFDDNGNFALSEDTAPTESLKVLHKTIKKMDEDINKFSFNTCVSACM
                     IAVNEFKKQNVNHRVILEPMLVLLAPFAPFITEELWHLAGHKDSVHKAQWPAFNEKYT
                     VDDVVAYPICINGKKRDLCHFAIDADDDTIIKESLERPDVIKWMEGKQAKKVIVVKGK
                     MVNIVV"
     gene            5612..6622
                     /locus_tag="PKV31_05810"
     CDS             5612..6622
                     /locus_tag="PKV31_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNB92403.1"
                     /translation="MQEAKDLLQLLRTPRNIVVTSHRNPDGDAIGSSLALKSVLEKKF
                     HNVKVVLPSEYPFDVAWLEGIDDIIIYDRDQGRATDAIAAADVVFCLDYNSLSRVDKV
                     GEAIAASGARIVLIDHHLYPENFADISISDYEVSSTCELVYEVCKTLGWVNDLTVQAL
                     EALLVGIITDTGSFKYSFSPKTLVNTSEIIRLGGDMVYVQDKLFNHLPEKSLRLLGHC
                     LSKRMVIYPDKKAGLIFLSKKDYIDFNIQRGDTEGIVNHLLALDDVNVAAFITEQPTI
                     IKISLRSKGAFSVQQMASEYFKGGGHKNASGGSYYGSLKSAISRFEEAVDKYKEEIIH
                     SY"
     gene            6643..7482
                     /locus_tag="PKV31_05815"
     CDS             6643..7482
                     /locus_tag="PKV31_05815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB92404.1"
                     /translation="MKKFSVLILFAMVMGMISCKNNAKDSVTKHGYPYTLFPTGSGDL
                     IQDDDVVFFDMQILHKDSILQDSRNNPIKPEFAIPADSLVTTPNPVIDALRLMRKGDS
                     VVVREKIDTVQGLPENMKDWKEITYRIKVTDVLNKKEKQKIKDMEPGVEKEVQKLITD
                     YNSGAIKDLKTTPTGLKYVILQDGNGPQIQKGEVAKVHYFGATVSNGKKFDSSFSRGS
                     TFTVPVGGGQVIPGWEEALLLMKKGTKAMVFIPGNLAYGANGIPGMIEPNSELAFYME
                     ILN"
BASE COUNT         2257 a         1422 c         1532 g         2312 t
ORIGIN      
        1 aggattatct caatgaatac tgttaccgat ataacaggca tcgcatgaaa ggagaaattt
       61 tggacgatat actttcaaga atgataggtc acaagcccag aacatggaag atgttgattg
      121 caagatgacc atttcaatat tgactttttt gactatttca catacagaaa tggctcttga
      181 ttaatcacgc caaacataag gaaataaaat aaaagccagg ctcaatagaa taatgtctat
      241 ggaacctaaa ataaggatat cagtagtgaa atcagtgtca ttcaataaac ctaaactatt
      301 ggcggataat ttcaaaagct ctaataacac tggaacaaca caagggaatc ccagaatcgc
      361 tgttaacatg tggctattac ctgtcttggc tcctatggca gatatgaatg agaatatgga
      421 ggcaaagcca atacctgcca ataaaattgt caacagaaat aatggatagt cgcgaacagg
      481 acttcctgcc acaaaactga ataaagccag tgtcaacaaa cctaacagta ctaataatat
      541 tgtattgaaa ataaacttgg cagcaaaaat ctgcaaaggt ccggctagtt gataaaaata
      601 aagtgcctta ttgctgttct cttgatcgaa actccttaat aacgcattaa ccgatacgaa
      661 tagaaagaca atccaaaaca atacattcca gttttgaggt tggatattta taaaagcgct
      721 atatataatg tatactgtag caaatacata taaaattatt gaactgatag aatgagcatt
      781 tctcatttca gagtaaaggt cttttttaac caaaagaaat acctttttca tcagaatcta
      841 aaatatttac tgaagcaaat ctataatata tttttgttga agaatgaaaa atagctcaga
      901 atggatttac attataaata aatgtaatat atttgtgatt ttatattagc tgtttgacgt
      961 caagactaaa tatcttattt tttatagctt gcctcttcgt ttcatttagc gcacagagtc
     1021 aacaatatgt tgttgctgaa ttacagaaag gagagggagg atatgcttta ctcagaaggt
     1081 atggattgga tatttctcca tgtaacctgg cggagtttta taggatcaat aacctacctt
     1141 acggttcagc tctttcattg ggaaagaaat acaatctgcc tgtcagaatt taccagtaca
     1201 ataaaagctc aattcggtct actgtaaaaa attttgattt ggtcaaagca cttgaagtag
     1261 agcgttataa taaatggttg aaatctcagc agattaagtc tgtttatttt ttgcaagatt
     1321 tgaaactttg ggtgcctttt tacatatata attgcaatta tgataacctt gctggtgcat
     1381 cacagcctga taatacacca gataagaatt taaacacaac gcaagcggaa gcaataaata
     1441 agactgaaat taagaagaat actttggata tagctttgtt tggaaataaa cacagtcagg
     1501 ttgaagtatt gggcaatcat ttacgaggac aagtctttta tattaagggt ggtcatggag
     1561 gtcccgatcc gggagcgatg gcgaaaattg atgacaaaat atattgtgag gatgaatatg
     1621 ccaatgatat tgctttaagg cttggaagaa aactgatcca gtacggggct atcgtccatt
     1681 tcattgtata tgatcctgat gatggcataa gagatgatga atatttacct tgtgacaagg
     1741 atgaattaca tgccggaaaa cgtgatattc aagtgaacca aatccaaaga ctcagaacac
     1801 gtgttcagat aatcaatgat tattatgaat cttatagaaa gaaaggtgtt aagaatcaga
     1861 agtttataag tattcatatt gatagtcgga atgaaagcct tgaattggat actcattttt
     1921 attaccagga agggtcaaag gtcggtcagg aaatggcagt caatacccag gctgtctttg
     1981 aaaagaaatt tggtgaattg ggaagcagaa aatacaccgg taccgttaaa gctcgagatc
     2041 tttatgtggt caaatattca aggccgccat gcctttttgt cgaggttgga aatattcaga
     2101 atgtcaatga ccaaaaacgt atcctggacc ctgacaaccg acagacattg gcagattggt
     2161 tgtatgaagg tataacgaaa tagtcgcata cctccagatt attcgggtgc ctgacatccc
     2221 ggaaattcac acaacgatat tgaccatttt gcctttgact actattacct ttttagcttg
     2281 cttcccctcc atccatttga ttacatccgg tctttcaaga gattccttta taatcgtatc
     2341 atcgtcagca tcaatagcaa aatggcaaag atcacgtttt ttaccattga tacatattgg
     2401 atatgccacc acatcatcta ctgtatattt ttcattaaaa gccggccatt gtgctttgtg
     2461 cacactatct ttatggcctg ccagatgcca aagttcttct gttataaatg gtgcaaaggg
     2521 tgccaataaa acgagcattg gctcaagaat tactcgatga ttgacattct gtttcttgaa
     2581 ttcgttcaca gcaatcatac aggctgacac acaggtattg aatgaaaact tattgatatc
     2641 ctcatccatt tttttaatcg tcttgtgcaa tacctttaat gactctgttg gggccgtatc
     2701 ctcactgagg gcgaagttcc cgttgtcatc aaaaaacagg ttccaaaaac gtcgaagaaa
     2761 tttgttgacc ccttcaattc ctttcgtgtc ccatggcttg gcttgctcaa gcggtcctaa
     2821 aaacatttcg tacattctga atacatctgt gccatgttct gaaattacag tgtccggatt
     2881 gatgacattg tacttggatt ttgacatttt ttccacctct gatttggtat tgaagcggat
     2941 ggcgtcagcg tcagttaccg ggtgaaaggt tccgttttga taatatccgc catatgtcac
     3001 aaagacagca cttgcaaatt ccggcctcca aaccgcaaat tcctccattg ccactttatc
     3061 aagataacca cccgtggagg caccgtaatc ctttactata tcaatatgga tactcatttc
     3121 agagaaacct tccatctcct tgtcttctga ggtgcttttc gccggataat cctgtattag
     3181 ctcatggctg acaaagacct gtaccgactg aggtaataat tttacctcgt attgtccgga
     3241 attattcttg aaatatagtt tgtctgttga ttccttaagt aagtaaatct tttcagatac
     3301 tccctgaatc atcccttggt tgattagcct cttgaatggt tcttcagtcg gtacaaggcc
     3361 gaaatcatac aagaacttat gccaggttct tgagtataac aaatgcccga ctgcatgttc
     3421 tgctccgcca atatatagat ccacgtccat ccagtaatct atggcattct ttgaaaatgg
     3481 caacgtgtca ttgtttgggt ccatataacg caagaaatac caggaggaac ctgcaaatcc
     3541 cggcatagtg tccgtttcac gcttcaagcc tggcgtagca ttgacccatt ctgttgctct
     3601 tgcgatgggt gagctgccgt cgccagtggg tttaaaatcc ttgatatcag gcaattccaa
     3661 tggtaatggg cttatttcat gcccgactcc atcttcgtca taggcgatag ggaagggctc
     3721 tccccagtat cgttggcggc tataattggc atctctgagc ctataattga tcaaacgctt
     3781 cccaaaacct tgtttttcta tggtttcaat agctttaatg atagcatcat ttacaggtag
     3841 cccatttagg aagtcggagt tgattaggat accgttcttt gattctatat cgtcgtcgtc
     3901 ttcatttctt tgaatgattt cacggatttc aaggttgaac gtttctgcaa acctcctgtc
     3961 cctttcatct cctgcaggta ctgccatgat ggcaccggtg ccgtattcaa tcaggacgta
     4021 gtttgagacc catattggaa tatcctttcc tgtaaagggg tgtatggcat atgaacctgt
     4081 aaatacacct gtcatctgtt tctgggcatt caatctttct tgttctgaag cctttgccgt
     4141 ttcacaagca taatcgaaca tcggtttttg ttgtgcctga atagtcagtt cattgacaag
     4201 tggatgttcc ggcgcaagta ccatgaatgt agcgccaaag attgtatccg gacgggttgt
     4261 gaaaatttca agattgtcaa ctctcccttt caaccgaaag aaaacccttg cgccttctga
     4321 ccttccgatc caattgcgtt gcatgatttt aagggaatcg gaccaatcaa catggtcaag
     4381 cccatccagc agcctctggg caaacgcggt agttcgcata ctccattgca tcataggttt
     4441 tttgattacc gggaagcctc ctctttcaga tacaccgtcc ttaacctcgt cattggccag
     4501 aacagtaccg agtgcttcac accagttgac cgtacttatt ttcctgtaca tcagtcggta
     4561 attcatcaac acatcgtatt gctccttggc ggaataattt ttccaatctg atgctgaaaa
     4621 ttggagtggg ctgttgcaag agctgttaat accttcagtt ccatgatttt caaaatgatc
     4681 gataaggata gaaatatctt ttgacttttc ttcactcttg tcataccagg cgttgtacat
     4741 cttgaggaaa atccattgcg tccatttata ataggaggga tcacaggtcc tgacctccct
     4801 ggaccaatca taagaaaatc caaggttgtc cagctgtttt cggaagctat tgatatttgc
     4861 ctgtgtgctt attgctggat gtacaccggt ttgtacagca aattgctcag ccggaaggcc
     4921 gaatgcatca taacccatgg gatgcagaac attgaaccca ttcaagcgct tgtatctgct
     4981 gaagatatcg gatgcaatgt aacctaatgg atgtccgaca tgaagccctg caccagaagg
     5041 gtaaggaaac atgtctagca cataatattt cggcttgtca ggatcagtat ggatcttata
     5101 tagttccgaa tctatccaga tttttttcca tttttcttca atttctgctg aattccactc
     5161 catttctatg cattttcaat tttgaagctg caaaggtaca aagaatacca tttatcaata
     5221 ctcaactaga gtgaagtcaa aggcaaaatt ttatgttttt gaagaaacat gacgttttaa
     5281 ttaattaaga agttgatcgg gagggtaatt gagtgtctat ttttattaaa attattatga
     5341 ttgctcatat tgtctggcaa ggtagccttt attccctggc ctggtgtctc ctgtgacgtc
     5401 tttagatctt ttctttccct ttttttcatt agaatctttg aaggatattc attgtaaagt
     5461 aaaacattcc tttatttaat ggagattcaa ctaaattaaa tttttttata tatagaaaaa
     5521 atacgatttt tgcggctgtt ttctcaacct tgaaaaatta atattattaa ttatctgtaa
     5581 ttcaggctgt taggaacagt tttgttttta tatgcaagaa gccaaagact tgttacaatt
     5641 gctccggact ccccggaaca tcgtggttac atcacacaga aacccggatg gtgatgctat
     5701 tggttcttct cttgcattga agtccgtcct tgaaaagaaa tttcataatg tcaaggttgt
     5761 tctgccttca gagtatcctt ttgatgtagc ctggctggaa ggtatagatg atatcatcat
     5821 atatgataga gatcaaggac gagctactga tgcgatagct gcggctgacg ttgtcttttg
     5881 ccttgattat aattcacttt caagggtgga taaggtaggt gaggcgattg cagcttcagg
     5941 tgccaggatt gttttaatcg accaccatct ttatcctgaa aattttgcgg acatttccat
     6001 ttcagactat gaagtcagca gtacttgtga gttggtttat gaagtgtgta agactttggg
     6061 atgggtcaat gaccttacgg tacaagcttt agaggcattg ttggtgggta tcatcacgga
     6121 tacaggttcg ttcaaatata gtttttcgcc gaaaaccctt gttaacactt cagaaatcat
     6181 aaggctaggc ggagatatgg tatatgtgca ggacaagttg ttcaaccatt tgccggagaa
     6241 atctctgcgt ttgctgggac attgtctttc caagaggatg gtgatctacc ccgataagaa
     6301 agccggtcta atatttttgt ctaaaaaaga ttatatcgat ttcaatattc aacggggcga
     6361 cacagagggt atagtcaatc atttgctagc gcttgatgat gtcaatgtag ctgcatttat
     6421 cacggaacag cctacgatca ttaaaatatc gcttaggtca aaaggggctt tttcagtaca
     6481 gcagatggca tcagagtatt tcaaaggcgg tggtcataag aatgcatccg gtggttctta
     6541 ctatggaagc cttaaaagcg ccatctccag atttgaagaa gcagttgata aatataagga
     6601 agaaataatt cattcatatt aacaataatc gtaatttatt ttatgaaaaa attttcagta
     6661 ttgatattat ttgccatggt catgggaatg atttcgtgta aaaacaatgc caaggatagt
     6721 gtgacaaagc atggttaccc ttatacactt ttccctacag gaagtggaga tctcattcag
     6781 gatgatgatg tggtgttttt tgacatgcag atcctgcata aggattccat tttgcaagat
     6841 tccagaaata acccgatcaa acctgaattt gcgattccgg ctgactcgtt ggttactact
     6901 ccaaatcctg taattgatgc attgcgtctg atgagaaaag gagacagtgt tgttgtacga
     6961 gagaaaattg atactgttca agggttacct gagaacatga aagactggaa ggaaatcacg
     7021 tatcgcataa aggttaccga cgtattaaac aaaaaagaaa agcagaagat caaggacatg
     7081 gaaccaggag tcgagaagga agttcagaaa ttgataactg attacaattc aggggcaatc
     7141 aaagatctta agacaactcc gaccggcctt aaatatgtga ttcttcaaga tggaaatggt
     7201 cctcagatac agaaaggtga agtagctaag gtacattatt tcggagcaac agtatcaaat
     7261 gggaagaaat ttgacagttc tttcagccgg ggttcaacct tcactgtacc tgttggtgga
     7321 ggtcaggtga ttccgggttg ggaagaagca ttgttgttga tgaagaaggg cactaaggca
     7381 atggtgttca ttcctggcaa tcttgcatac ggcgccaatg gtattccggg aatgatagag
     7441 ccaaattctg agcttgcatt ctatatggag atactgaatt gatgacagaa tttttcgatg
     7501 tagcatcagc ctctatctgt cag
//
LOCUS       DAOQMC010000114         7431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4059_length_7431_cov_4.839751, whole genome shotgun sequence.
ACCESSION   DAOQMC010000114 DAOQMC010000000
VERSION     DAOQMC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4059_length_7431_cov_4.839751"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKV31_05820"
     CDS             complement(<1..1042)
                     /locus_tag="PKV31_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB92405.1"
                     /translation="MDISFLKTLGINEINPGTTTGTIWFEPEEYLDSYSPVNGKLIGK
                     AGITTEAQYEDIINKSTEAFKFWRNIPAPKRGEIVRQYGDVLRANKDALGRLVSYEMG
                     KSLQEGWGEVQEMIDICDFAVGLSRQLYGLTMHSERPGHRMYEQWHPLGIVGIISAFN
                     FPVAVWSWNSMIALICGDVCVWKPSEKVPLSAIACHKLLEEVLRKNNIPEGVTCLING
                     DYRIGELMTKDPRVALVSATGSVRMGKIVGATVAARLGKSLLELGGNNAIIVTPDADL
                     NIVLTGALFGAVGTAGQRCTTTRRLIIHESHYENVKQKLVKAYGQLVIGNPLDAGVHV
                     GPLIDKDAVDLYN"
     gene            complement(1586..2380)
                     /locus_tag="PKV31_05825"
     CDS             complement(1586..2380)
                     /locus_tag="PKV31_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92406.1"
                     /translation="MELNDKWVWVTGASSGIGKAIAIELSFHKANLVLMSRNVSELKK
                     TADACNPNVNIIIQPIDLADNASIDEAVRVLLHDKKIPPHILINNGGISQRATALDTV
                     DAVTRKIFDINFFGTVYLTRALLPRMIEMGGARIAVITSLVGIIGSPKRTSYSASKHA
                     LHGYFDSLRAELYKEQIGVTLICPGFIRTNISLSAVTGDGTAQNTMDEKTGNGMDPAK
                     LAKIAVRGIMREKEEIYIGGKEILAIYLKRFVPTIFSKILRKTKVT"
     gene            complement(2451..3533)
                     /locus_tag="PKV31_05830"
     CDS             complement(2451..3533)
                     /locus_tag="PKV31_05830"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HNB92407.1"
                     /translation="MKNPFFKYLAFLSIMMIPLLFARCKEDANDTNAGKKELLRGIDI
                     SFLPQIRQYGISFKNQSGQEGDLLKIVKENGVNLVRIRLWTGNPGKHSGEDEVYALAE
                     ECRNQGFKILLDLHYSDTWADPGHQTIPASWIGLDINQLKDSVYRYTFRVTSKCRPDY
                     IQIGNEINAGLLWPTGHISRPASMDELLKVGCNAARDAHPAIKILLHYAGIDGSIDFF
                     KKRNETGIDYDIAALSYYPYWHGSTIADMGNTLNDIRFYTKKEVMIVEFAYPFTLKWS
                     DHTNNVIGLESQLSPGYPATAEGQAVFVRTIRTVCEKYKAKGLCYWGGEWVAFKGDTS
                     SNGSSWENQALFDFDFRANPALTEYR"
     gene            3864..4592
                     /locus_tag="PKV31_05835"
     CDS             3864..4592
                     /locus_tag="PKV31_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB92408.1"
                     /translation="MKVALFVPCYIDQFFPQVAKATLHLLEKLGCTVEYPSNQTCCGQ
                     PMANAGYEYLAEGCADIFRQCFDRYEYIVSPSGSCVLHVKDHVMPRSNESDGPAVMEL
                     SEFLTDVMEITSLDASCPYRVGLHNGCHAIRGLGLSSMSEVPGNRFSKPGSLLRMVNG
                     LELVALDREDECCGFGGTFSVFEETVSVRMGNDRIDDHIRHGAQYITGTDMSCLMHLE
                     GLLRRRKCPVRVVHLAEILNDEIH"
     gene            4576..5988
                     /locus_tag="PKV31_05840"
     CDS             4576..5988
                     /locus_tag="PKV31_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HNB92409.1"
                     /translation="MTKSIEAVNNRHNSEPHSKLAAAFNKDAARVGWHDETLWWVRAK
                     RDKMAYQLPEWEALRQAASEIKSHTLANLGEYLVEFEQKAVENGLVVHWAVDADEHNR
                     IVHGLLKGNGIDRIVKSKSMLTEECGLNEYLLKQGIDVIDTDLGERIVQLAGEAPSHI
                     VLPCIHKKKEEIGDLFAEHLDTPKGCSDPKILTEAARQHLREAFLSRRAAITGVNFAI
                     AQTGEFVVCTNEGNADMGAHLADLHIACMGIEKIIPRRKDLGIFLRLLARSATGQPIT
                     TYSSHFRKPDRERKIHIVIVDNGRSKQLAREQFRNSLKCIRCGACMNTCPVYRRSGGH
                     SYHTAVAGPIGSILAPNLDMRQYADLPFASTLCGSCTHVCPVKIDIHDQLYRWRQVLV
                     EKGYTTTSKTIVMKGMRTVLSSPMIYHTAAKAGRAILSNAPFLIGNRFNPWFQGREMP
                     KPPKQSFGEWYRQNRAKDGE"
     gene            5978..6580
                     /locus_tag="PKV31_05845"
     CDS             5978..6580
                     /locus_tag="PKV31_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNB92410.1"
                     /translation="MENNARNIILKAVNLANEGLVRPDLPDPHIDSTTYDDAVTRFIT
                     VLEDIGGKAVRVATMEEVHTGVASMFDLSGMRVFSAGFEQGRFELPLETSIGPRRYSD
                     IGLLITTSDLGVAENGAIWLDESKVPDRILPFIAENLVVILSGDAIVHNMHHAYDKLG
                     EAAVGFGVFIAGPSKTADIEQSLVLGAHGARTMTVFIVGA"
     gene            6831..>7431
                     /locus_tag="PKV31_05850"
     CDS             6831..>7431
                     /locus_tag="PKV31_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92411.1"
                     /translation="MRAYFLFPIFLTLISCSTSNNKAFINNVQKSSCNQENSYAYTKS
                     DIPIPLQELKIDSSLFMYFNERDLNVANAIGVIDVLSDYIQKKGRNRDAASPEQKIEW
                     IQIRQELNDRIDLASLEISAVTAELDCEEERLNQIASFLNEKEKRKESNLTVTSIILG
                     AVSAIVTGVLVSKKDQSNTADIMGVGVGIADVTLGILLLT"
BASE COUNT         1974 a         1547 c         1751 g         2159 t
ORIGIN      
        1 agttatacaa atcaaccgcg tccttatcga tcaggggacc tacatgaact cccgcatcca
       61 gtggattacc gatgacgagt tgtccgtatg ccttcacaag tttttgcttg acattttcat
      121 agtggctttc gtgaataatg agtcttctgg tagtggtaca acgctgtccg gcagtaccta
      181 cagctccgaa taatgctccg gtgagcacaa tattgagatc tgcatcaggg gtgacgatga
      241 tggcattatt tccacccaat tcgagcaaag atttaccaag gcgtgcagct actgtcgctc
      301 ctacaatctt ccccatcctg acactaccgg ttgccgatac aagggctact ctcggatcct
      361 tggtcatcaa ttcgccgatt ctgtagtcac cattgataag acaggttact ccttcaggaa
      421 tattattttt cctgagaact tcttcaagta atttatgaca ggcgatggca ctcaacggaa
      481 ctttctcaga aggtttccat acgcatacat ctccacaaat aagggcaatc atggagttcc
      541 atgaccatac cgctacggga aagttaaaag ctgaaatgat gcctacgatg cccagaggat
      601 gccattgctc atacatacga tggccgggac gttcactatg catggttaga ccatatagct
      661 ggcgggataa tcccactgca aagtcacata tgtcgatcat ttcttgtact tcgccccagc
      721 cctcctgaag gcttttaccc atttcataag atacaaggcg gccaagagca tctttattgg
      781 ccctcagtac atcaccatat tggcgcacta tttcgcctct tttgggtgcc ggaatatttc
      841 gccagaattt gaaggcttcg gttgatttgt tgataatgtc ttcgtattga gcttctgtag
      901 taatgccggc cttaccaatc agcttgccat ttacaggtga ataactgtca aggtattctt
      961 caggttcaaa ccaaatcgta cctgttgttg tgccgggatt tatttcattg attcctaatg
     1021 ttttcaaaaa ggatatatcc atatcttaat gtttattttt ttgaggtgca aaattacgat
     1081 aagaaaaccg attaaacaat tatattccct tgtttcaatg gatattctga ttttatgttc
     1141 agggtgattt tttaatatcg gttaaaatgt tttaggtctt cgttattttg gcacctgtat
     1201 aatatactta tcaatttctt ttgagcaact ttacgttcag tttgacaacc tgtattttcg
     1261 cctacatcca taaatgaaga tcctgaacat ggttctttat ttatcccttc ggaatagatg
     1321 agtcttccct tcaggataaa gggggctttc tacaagaatt ttcaatcgtt tgaatggtag
     1381 tttaatttta ttgggtcatg ccaatagggt tggaataccg gcaatctcac cttggttccg
     1441 gaagggcgta ctgataataa ttggagtact atgttttttt tgtacctatg gttgaaaaga
     1501 tctgatacga aatatttaca cggatatctg accggcacag tagcggtttt ttggcctcac
     1561 tgaatttatt aatgtttctt gtaggtcagg ttaccttcgt ctttctcaat attttggaga
     1621 aaatggtcgg aacgaatcgt tttaagtata ttgcaagtat ttccttgcca ccgatataga
     1681 tttcttcctt ttcacgcatg atcccccgga cagcgatttt ggccagtttg gcgggatcca
     1741 tgccattgcc tgtcttttca tccattgtat tctgtgcagt tccgtcaccg gtgactgccg
     1801 agaggctaat attggttctt atgaaacccg gacatatcaa tgtgacgcct atctgttctt
     1861 tgtacaattc agcacgcagg ctgtcgaaat atccgtgcag agcgtgtttg ctggcagaat
     1921 agctggttct cttggggctt ccaatgattc caaccaggct ggtaattacc gcgattctgg
     1981 ctcctcccat ttcaatcatc cttggcagta gtgccctggt aagatatacc gtgccgaaaa
     2041 agttgatgtc gaatatcttg cgggtcactg catccacggt gtccagcgct gtggcccgtt
     2101 gactaatacc tccgttattg atcaggatgt gtggtgggat ttttttgtca tgcagcagta
     2161 ccctaaccgc ttcatctatg gaggcattgt ctgccaaatc aataggttgt ataatgatat
     2221 tgacatttgg attacaggca tcggcggtct tttttagttc actgacattt ctgctcatga
     2281 gcacaagatt ggctttgtga aaagaaagtt cgatagcaat agccttgccg attcctgagg
     2341 aagcaccggt gacccacacc catttatcat ttaattccat tggaagtatt tttttgcaaa
     2401 tatggatatt tcaattcaaa ttgtaatacc taattgatcc attgcagtgt ttacctgtat
     2461 tcagtcaaag cgggatttgc cctgaagtca aagtcgaaca atgcctgatt ttcccaggaa
     2521 gagccattgg aggaggtgtc acccttaaag gcaacccatt cccctcccca atagcagaga
     2581 ccctttgcct tatatttctc acacactgtc cgtatggtac gtacaaagac cgcctggcct
     2641 tctgcagtgg ctgggtagcc cggtgataac tgactttcca gaccgatcac attgttggta
     2701 tggtcactcc atttcaaggt aaatggatag gcaaattcaa caatcattac ttctttcttt
     2761 gtgtaaaaac gtatgtcatt caaggtattt cccatgtcgg ctattgtgct tccgtgccag
     2821 taggggtagt acgagagggc agcaatatca taatcaatgc cggtttcatt tctctttttg
     2881 aaaaaatcga tgctgccatc gatacctgca tagtgaagta ggatttttat ggcaggatgg
     2941 gcatctcgcg ctgcattaca accgactttc aacagttcgt ccatagaggc aggccggctg
     3001 atgtggcctg ttggccaaag tagacctgca ttgatttcat tgccgatctg gatatagtcg
     3061 ggacgacact tcgaggtgac ccggaaagta tagcgataca cggaatcctt gagttgatta
     3121 atatccaggc cgatccagga agcaggaata gtctggtgcc ccgggtcggc ccatgtgtcg
     3181 gaataatgca ggtcaagtaa gatcttgaat ccctgattgc gacactcttc ggcaagggca
     3241 tacacttcat cctctccgga atgtttcccg ggattacccg tccaaagtct aatcctgacg
     3301 agattgacac cattttcctt tactattttg agcaagtcgc cttcctggcc ggattgattc
     3361 ttgaaagaaa taccgtattg cctgatttga ggcagaaaac tgatatctat gccccggagc
     3421 aattcctttt taccggcatt tgtatcattc gcatcttcct tgcatcgggc aaagagaagt
     3481 ggaatcatca tgatgctcaa gaaagcaaga tatttgaaaa acggattttt catccttcaa
     3541 aggtaaaata agatgaggaa aatggtttct cccggattat tatttttata tttaacgtat
     3601 tacctgatga tggttagttt aaatattgtt taggtaaatc attttgatgc aaacgcatat
     3661 caaaaaatta ttctcaaatc aaccggatag ctttgtgaat tgactttatg aaatcttact
     3721 tatatcacgt tggaacttca tcggtaaaga tctggttttg aaatcactta attttcaaaa
     3781 gggtacattt cgttgtacat ttatgttttt aatccattct tccgaaaatg attaagtttg
     3841 tattgccgga ttaggcattg attatgaaag tcgcattatt cgtaccgtgt tatattgatc
     3901 agttctttcc acaggtcgca aaggccactc tgcatctgct ggagaagttg ggctgtacgg
     3961 tggaataccc atccaatcag acatgttgcg gtcagccgat ggccaatgcc ggttatgaat
     4021 atttggcaga aggatgtgct gacatctttc ggcagtgttt tgacaggtat gagtacattg
     4081 tttccccatc cggtagttgt gtacttcatg taaaggacca tgtcatgccc aggagcaatg
     4141 aatcggatgg ccctgcagtc atggaattgt cggaatttct tacagatgtt atggagataa
     4201 cttccttaga tgctagttgt ccttatcggg taggcctgca taatggatgt catgcaattc
     4261 gcggtcttgg tctaagttcg atgtctgaag tgccgggtaa tcggttttcc aaacccggtt
     4321 cccttctaag gatggtgaat ggattggaac ttgttgcact cgaccgggaa gatgaatgtt
     4381 gcggatttgg cggtactttt tccgtcttcg aagaaactgt atctgtaagg atgggcaatg
     4441 atcgcatcga tgatcatatc cgtcatggcg cacaatacat caccggcacg gatatgtcct
     4501 gcctcatgca tcttgaaggc ttgctgaggc gaaggaagtg ccctgtcagg gtcgtgcatc
     4561 tcgctgaaat attaaatgac gaaatccatt gaagcggtga ataatagaca caacagcgaa
     4621 ccccacagca agttggctgc ggcattcaac aaggatgcag cccgtgttgg atggcatgat
     4681 gagacactgt ggtgggtaag ggcgaagcga gacaagatgg cttatcaatt gcctgagtgg
     4741 gaagctttac ggcaagctgc ctctgagatt aaaagccata cattggccaa tttgggtgaa
     4801 tatctggtgg aatttgaaca aaaagcagtt gaaaacggcc tcgtagtcca ttgggcggtg
     4861 gatgcggatg agcacaacag gattgtgcac gggcttttga agggaaacgg aatagacaga
     4921 atcgtgaaga gtaagtccat gttgaccgag gaatgtggct tgaatgaata tcttctcaag
     4981 caggggatcg atgtaattga taccgacctg ggtgagcgaa tagtgcagtt ggcgggtgag
     5041 gcaccgagcc acattgttct gccttgcatc cacaagaaaa aagaagagat cggtgatctt
     5101 tttgcggagc atctggacac gcccaaaggt tgctctgatc ccaagatact tacagaggct
     5161 gcccgacagc atcttaggga ggcattccta agtcgtcggg ccgccatcac aggcgtcaat
     5221 ttcgccatcg cgcaaaccgg tgaatttgtc gtgtgtacca atgaaggcaa tgccgatatg
     5281 ggtgcccatc ttgccgactt gcatatagcc tgtatgggca tcgaaaagat tattccccgc
     5341 aggaaggatc ttggtatttt tctgcgtctg ctggcacgta gcgctaccgg ccaacccatt
     5401 accacttata gcagccattt ccgcaagccg gatagagaga gaaagataca tattgtcatc
     5461 gtggacaatg ggcgtagtaa gcagttggcc agggaacagt ttcgcaattc actcaaatgc
     5521 atccgttgtg gggcctgtat gaatacctgt cctgtatata ggaggagtgg gggccacagc
     5581 tatcatactg cggtagccgg gcctattggc tccatcctcg caccgaatct tgatatgagg
     5641 caatacgctg atcttccttt tgcgtcgacc ctgtgtggct catgtaccca tgtatgcccc
     5701 gtcaagatcg atatccacga tcagctctac cgatggcggc aggtgctggt ggagaagggg
     5761 tacaccacca catccaagac tattgtcatg aaaggaatgc gcacggtgtt gagttctccg
     5821 atgatttatc acacagccgc aaaagccggc agagcaatat tgagcaatgc gccatttctg
     5881 atcgggaacc gtttcaaccc atggttccag ggaagggaga tgccgaaacc tccaaaacag
     5941 tcgtttggcg aatggtatag acaaaaccgt gcaaaagatg gagaataacg ctcgcaatat
     6001 aatattgaaa gcggtgaatc ttgccaatga aggcctggta aggccggact taccggatcc
     6061 gcatattgac agcacaacgt atgacgatgc ggtgacgcgt tttataactg tcctggaaga
     6121 tattggtggt aaggcggtca gagtggctac gatggaagag gtgcatacag gagttgcatc
     6181 catgtttgat ctgtccggga tgcgtgtttt ttcagccggc tttgaacaag gccgtttcga
     6241 attgccttta gaaacaagca tcgggccaag aaggtatagt gacattgggc tgttaatcac
     6301 cacttccgac ttaggtgttg cggagaatgg cgctatttgg ctggatgaaa gcaaggtgcc
     6361 tgacagaata ttgccattca tagctgaaaa tctggttgtt atcttatccg gggatgccat
     6421 cgtacacaat atgcaccatg cgtatgataa gctgggtgaa gcggcagttg gatttggtgt
     6481 atttattgca ggaccatcga aaactgctga tattgagcaa tcgctggtgt tgggagctca
     6541 cggagccagg acgatgactg ttttcattgt gggagcatga aagggattgg aattatagat
     6601 ctgtatttgt atcaagaaat tccctcaatg gtagagtacg accgaatgtc gcattcgaat
     6661 aacattgaag atatagccgt aatgtatgat gaaaaagtgg ctggatagtg attttcaggg
     6721 agtttgacgc tgattatagt atgtagaatc atgaccttat aaataaaaca tctataatcg
     6781 gacctaattc attgtgaagt agtgtatttt gcaacaaaaa tacaggtttt atgagagctt
     6841 attttctatt tccaattttt ctcacactta tttcctgctc gacatcaaat aataaagcat
     6901 ttatcaacaa tgttcagaaa agttcttgta accaggagaa tagttatgct tataccaaga
     6961 gcgatattcc aatccccctt caagagctga aaatagattc atcgcttttc atgtatttca
     7021 atgaaaggga tttaaatgtc gcgaatgcca tcggtgtgat tgatgtattg agtgattata
     7081 ttcaaaaaaa gggcagaaat agggatgctg cctctccgga gcaaaagatc gaatggatac
     7141 agatccggca ggaactgaat gaccgaatag acctggcttc gctggagata tcagcagtaa
     7201 ctgctgagtt ggattgtgaa gaagagcgct tgaatcagat agcttctttt ttaaatgaaa
     7261 aggagaagcg aaaggaatcg aacctgacag taacatccat cattcttggt gctgtcagtg
     7321 caattgttac cggagtcctg gtgtcaaaaa aggatcaaag caacactgcc gatattatgg
     7381 gcgtcggagt gggtattgca gatgttacac tcggaatact acttttgaca a
//
LOCUS       DAOQMC010000115         7386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4090_length_7386_cov_4.720093, whole genome shotgun sequence.
ACCESSION   DAOQMC010000115 DAOQMC010000000
VERSION     DAOQMC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4090_length_7386_cov_4.720093"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKV31_05855"
     CDS             complement(<1..242)
                     /locus_tag="PKV31_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HNB92412.1"
                     /translation="MSIVEMRVPTIGESINEVTLSQWLKADGAIINMDEPICEFESDK
                     ATLEFPAEAAGKLIYVAGEGDDLKIGDLVAKIDTSFA"
     gene            complement(914..3334)
                     /locus_tag="PKV31_05860"
     CDS             complement(914..3334)
                     /locus_tag="PKV31_05860"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB92413.1"
                     /translation="MITSISQIYSQGFLHFKPNNEALSPVLRQKMPNTKVVNVDIRKL
                     YNSIVDNRQVLTIEAESSNWTFRLEENSLISPDYFTTIAGASGNVVSRDISTKTYFGV
                     AEGRSASISLTIDHDFINAMIVDGTKTLFIEQEWIMDHTADRSSVLLYDASEVKPDGS
                     FSCGVTALHEHKNDYHEMLEARTGQCKQVELAIATDASMYTKFSNSVTNVENHNISVM
                     NNVAFNYRHEFVENIEFTIVTQYISTSYANDPLTPNTASTNANTVLPRFSTWSYNGGF
                     GVAHDLGQFWTNRDFDGSTVGLAYVGTVGVTGYKYHILQDFTSDPTSLRVMTAHEMGH
                     NFGAGHDNSGDPYIMAPSVGVSNDWSSLSKTTINNEIPTYTSLSDCTGSVSAYFAMQP
                     GSICGGGSVYYKDKSVNGSTRTWTFAGGTPSTSTDQQPVVTYNTVGTYSALIVSGNSS
                     YGLANAVIVGNAPALNQNACPLPTNPPGNAGLQMFALNGMTSNSGVGSDDGSIYVDRS
                     CINIATLEPSTTYTMSFTVGNCASNPIIYENIKAYIDYNDNGEFGGGELVVSSSSNYY
                     CGTVNFNFTTPASPIMNKLLRLRVITSPSGVSGPCQNITNGQVEDFSIIFKQNIPLPL
                     NLLSFTGRNEGTVNILQWSGIDERNVREYLVERSRDGKLFSPIGFMTAQNKPSVSYTF
                     TDISAETNHLQYYRLMIIDQSGNVSFSKIVTIDAGKRSFTLDRLIRLIPAGGKLSGNI
                     ICQKQGKIEIGLFDMLGKPLVLKTITVGIGVEKLEINMADFAAGTYILTLRDESGTVI
                     TEKVVKGE"
     gene            3941..5197
                     /locus_tag="PKV31_05865"
     CDS             3941..5197
                     /locus_tag="PKV31_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB92414.1"
                     /translation="MDLFERLKASDKGHLGRYSEEADGYYMFPKLEGELGPRMTFNGK
                     EVINWSINNYLGLANHPEVRAVDAQAAKDWGLAYPMGARMMSGNTRLHEQLESELAEF
                     VHKESSILLNYGYQGVVSAIDCLVSRHDVIVYDSESHACIVDGVRLHLGKRFAFDHNN
                     MESLRTNLQRAKNIIDETGGSILVISEGVFGMRGDQGKLKEIVAMKKEFPFRLFVDDA
                     HGFGALGKNGEGAGVEQGIQDEIDIYFSTFAKSMASIGAFIASDKFVVDYIRFNIRSQ
                     IFAKSMPMPLVVGNLKRLDMIRRSPEFKNKLWDNVNYLQNGLKSRGFDIGSTNSCVTP
                     VYLKGSIEEAMVLVHDLRENYGIFCSMVVYPVIPKGMIILRLIPTAVHSKEDIDQTLE
                     AFSMIKDRLYSGEYAKIAAERYVAKT"
     gene            5243..6301
                     /locus_tag="PKV31_05870"
     CDS             5243..6301
                     /locus_tag="PKV31_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB92415.1"
                     /translation="MKPYISGIQQIGVGVADVHEAWKWYHRAFGMDIPVFEEAATAAL
                     MLPYTGGKPQDRHAILALNLRGGGGFEIWQYTSRTPQKAAFEVKAGDLGIFIAKIKTS
                     DIKKAFSHLIDCGATMLSEPAQSLNGMRHFYAKDPYDNLFEIIEQKDWFSSGPGVFGG
                     CCGAVIGVSDIEKSKLYYKALLGYETISGETEVMDNEWRNIPGGEKKFKRAILTHEYP
                     RKGPFSKLLGYTSIELIQALDYQPNKIFENRFWGDLGYIHLCFDIQGMDEMRKLCEQF
                     GHPFTVDSSNSFDMGEAAGHFSYIEDPDGTLIEFVETHKIPIIKKIGWYLNLRNRPAD
                     KALPNWMLKALRFSRREL"
     gene            complement(6342..6953)
                     /locus_tag="PKV31_05875"
     CDS             complement(6342..6953)
                     /locus_tag="PKV31_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNB92416.1"
                     /translation="MYPLFESIRVENSIIMHPSWHLMRYLDSYTTYYGYEPANGLFDG
                     LIIPEACHAGTFKLRISYNHNSRKATWEPYTIRDITSLRLIEANDIDYSLKYTDRSHL
                     NRLFKQKKDCDDILIIRHGLITDSSYCNIVLFNGEEWHTPAGPLLCGTARQRLLASGQ
                     VIARDISVDDLRSYECFKLINAMRDFETLPSIAVDFIRIDDEC"
     gene            complement(6937..>7386)
                     /locus_tag="PKV31_05880"
     CDS             complement(6937..>7386)
                     /locus_tag="PKV31_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNB92417.1"
                     /translation="NTIVDLMRNDLAMVAHDIRVIKYRFVQRIKTYKGEILQTSSEIA
                     GRLKHNWRTRLGEMLTLLLPAGSVSGAPKQKTLDIIRTAELGPRGYYTGVFGYFDGHN
                     LDSAVAIRYIEQGIDGLLFRSGGGITAQSNEEDEYLEMIQKIYVPTL"
BASE COUNT         2022 a         1542 c         1680 g         2142 t
ORIGIN      
        1 gcaaaagaag tgtctatctt cgctacaagg tcgcctatct tcaggtcgtc accctccccg
       61 gcaacataaa tcaattttcc ggctgcctct gcagggaact ccagtgttgc cttgtctgac
      121 tcgaattcac atattggttc atccatgttg atgatggcac catctgcctt aagccattgg
      181 gagagtgtta cttcattgat ggattctccg atggtgggca cacgcatttc gacgatactc
      241 atagttgatt ttttagattg atctaaatat ttttttaagc atgttaaatt ttgtctaaca
      301 cgctaagtta gaacattatt ccatctcaaa caaaagaata tcggggaagt tttaaggaat
      361 cgataaaaag ggatgcatta atgcgttttt tctgaagtaa aacaaattct tgcacatcaa
      421 aatgctggtc ggaagttcct ttccggtcag cattctgact ccacattagc agcttttatt
      481 tatagtttta tcaacctcct tgctaactat gaccccttat gcctgtcaat catgcaaatt
      541 taaatccttc atagcgactc aaaccaacac attaatttct tttaaggctc aacggttttc
      601 atttttcaaa tgcctgtatc gaaaattttg actgatggag tatcttccaa tcccggacag
      661 gttgtaatat cgtttgtgaa gttttgacag gtgagcactg ctcctttctg atcaagggta
      721 tgagtcacat gcaggcatag atatagttat cgttcccttt caatagcttc attcagtagc
      781 aataccttcc cttataggga cataaatctt cccggctcct gcatcttcta tcgaagtccg
      841 gtttcttccg gaaaactttt ttcaggctta catcccaccg tattttcttc aaggggcgta
      901 tcttgtaatg cttttattct cccttaacta ctttttccgt aatcaccgta cccgactcat
      961 ctctcagagt aagaatatat gtcccggcag caaaatcggc catatttatt tcaagttttt
     1021 cgacccctat tcctacagtg atggttttga gcaccaaagg tttgcccaac atgtcaaaca
     1081 agccgatttc tatttttcct tgtttttgac atataatatt cccggacaac ttgccacctg
     1141 ccggaatcaa cctaatcaag cgatcgaggg tgaagctacg ctttccggcg tctattgtca
     1201 caatcttgct gaaggagacg ttgcccgatt gatcgatgat catcagacga taatactgca
     1261 agtgatttgt ttcggcagat atatctgtaa aagtatagga tacagatggt ttattttggg
     1321 ccgtcatgaa gccgattggg ctgaagagct taccatcccg acttctctcg acaagatatt
     1381 cccggacatt tctttcatca atacctgacc attgaagtat attgactgtt ccttcatttc
     1441 tgccggtaaa tgacagcagg tttagtggta gcggaatatt ttgtttaaat atgatggaaa
     1501 agtcctcgac ctgaccattg gtgatgtttt ggcacggtcc tgaaacgccg gacgggcttg
     1561 taatcaccct gagtcttaaa agtttattca taattggact tgcgggtgtt gtaaagttaa
     1621 aattcactgt gccacaatag taatttgaag aactggaaac cactaattct cccccaccaa
     1681 attctccgtt atcattgtaa tcaatataag ccttaatatt ttcataaata atgggattac
     1741 tggcacaatt tccaacagta aatgacatcg tataggtggt ggaaggttcc agggtagcaa
     1801 tattgataca tgaacgatcc acataaatac tgccatcatc tgaaccgaca ccactgttac
     1861 tcgtcatccc attgagtgca aacatctgta atccggcatt gccgggagga ttggttggca
     1921 agggacatgc attttgattc aaagccggag catttccgac tataacagca ttggctaaac
     1981 cataggagct attgcccgac actatcaatg cagaataggt tccgactgta ttgtaggtta
     2041 caacgggttg ttgatcagtg ctggtcgatg gggttccacc tgcaaaggtc caggttcggg
     2101 tgctcccatt gacggactta tccttatagt ataccgatcc gcctccacag attgatcccg
     2161 gctgcatcgc aaagtatgca cttacactgc ccgtgcaatc agacaggcta gtataggtgg
     2221 gtatttcatt atttatcgtt gtttttgaca aagaagacca atcattagag actccaacag
     2281 aaggagccat gatataggga tctccagaat tatcatgacc cgcaccaaaa ttatgaccca
     2341 tttcatgtgc cgtcatgacg cgtaagcttg taggatcaga agtaaaatcc tgaagaatgt
     2401 gatacttata tccggtgacc ccaaccgttc ctacataagc aaggcctact gtcgagccat
     2461 caaaatcacg gtttgtccaa aattgcccca aatcatgagc tactccgaat ccgccattat
     2521 aagaccacgt agagaatctt ggcaggactg tatttgcgtt agttgatgct gtattggggg
     2581 tcagcgggtc attggcataa gatgtgctga tatattgggt gacgatagta aattctatat
     2641 tctcaacgaa ttcgtgtctg tagttgaaag ctacattgtt catcacggaa atattatggt
     2701 tctcaacatt tgtcacagaa ttgctgaatt tggtgtacat ggaggcatcg gtagcaatgg
     2761 ctagttccac ttgtttacat tggccggttc tggcttccaa catctcatgg tagtcatttt
     2821 tatgttcatg caacgctgta acaccacaag agaaggagcc atccggctta acctcactcg
     2881 catcatataa gaggacggaa ctgcgatcgg ccgtatggtc cattatccac tcctgctcta
     2941 taaacagggt ctttgttcca tccacaatca tcgcattgat gaagtcatga tcgatggtta
     3001 gactgatgga agcgcttcgt ccttctgcta caccaaaata tgtttttgtg ctgatgtctc
     3061 tactgactac attgccggaa gctcctgcta ttgtagtaaa ataatcagga gaaatcaaac
     3121 tgttctcttc caggcggaat gtccagttac tactttcggc ttctatagta agaacttgtc
     3181 ggttatcgac tatggagtta taaagtttcc gtatatcgac atttactacc ttagtatttg
     3241 gcatcttctg acgcagtacc ggacttaatg cctcattatt gggtttgaaa tgaaggaagc
     3301 cctggctgta tatttgactg atggacgtga tcaaaaaaat gaaaataaca ataattccgg
     3361 ttcggtacat aggtagtact tttgattgat taaacaaaaa tgaagtgtgt gcttttgggc
     3421 gggagattga agtcaaggcg attcaggccg gaatgtgcta agagcgtgga tccaaaccta
     3481 tggatgcctt catggttatg gccgggtgag ttgcaaatcc aattggacaa agcaatatat
     3541 taatattaat acgaatatat aactttctcc tgaaaatgcg tatataaaat tatgttatta
     3601 acaaaaaatt tgttgaataa taagaatacc ttgagttctt tgataaaaat ataatcaaaa
     3661 aacttatact ttagtttgaa tctgaatttg ttgatcggca tggggaagat gtccatgtat
     3721 accgaggtaa aagggatatt cagttattga ttggcttcca atttttagtg aatagcatgt
     3781 cgccggagtt attaatttga caggaatcat gtctttagag ttttatatcc cttgaggcaa
     3841 tatttgccct tttatcttcg gatcgttcaa tacgttcaga tttcttttcc taatctcctt
     3901 aatttttatc aactttgcgc cttacaattt taatttaaaa atggatttat ttgaaagatt
     3961 gaaagcttct gacaaaggcc accttggccg atattcggaa gaagctgacg gatattatat
     4021 gtttcccaag ctggaaggcg aattaggtcc ccgaatgacc tttaacggaa aggaagtgat
     4081 caactggagt atcaacaatt atctcggtct ggccaaccac ccggaagtac gcgccgtaga
     4141 tgctcaggct gccaaggatt ggggcctggc atatccgatg ggtgctcgta tgatgagcgg
     4201 taacaccagg ctgcacgagc aattggaaag cgagctggct gaatttgtcc ataaggaatc
     4261 gtccatcttg ctcaattatg gatatcaggg cgtagtttct gctattgatt gtcttgtaag
     4321 ccgacacgat gtcattgtat atgattcgga aagccatgcc tgtattgtag atggtgtcag
     4381 gttgcatttg ggtaagcggt ttgccttcga tcacaacaat atggaaagcc tccgtactaa
     4441 tctccaacgt gccaaaaaca tcatcgatga gacaggtgga agcattttgg tgatttcaga
     4501 aggtgtattt ggcatgcgtg gagaccaggg taagttgaaa gaaatcgtag ccatgaagaa
     4561 ggaatttccg tttcgactgt ttgtggatga tgcgcatgga tttggtgccc tgggcaagaa
     4621 tggagagggt gccggtgttg agcagggaat ccaggacgaa atagatattt atttttccac
     4681 ctttgccaag agtatggcca gtattggcgc ctttattgcc agtgataagt ttgtggtgga
     4741 ctatatccgg ttcaacatca gaagtcagat attcgcgaag tctatgccca tgccgcttgt
     4801 agtgggcaac ctgaagagac ttgacatgat ccgccgaagc cccgaattta agaataagct
     4861 ttgggacaat gtcaactacc tgcaaaatgg ccttaaatca agaggattcg atatcggctc
     4921 cacgaatagc tgtgtgactc ctgtatattt aaagggcagt atcgaagagg ccatggtatt
     4981 ggttcatgat ctgcgagaga attacggaat attctgctcc atggtggtat atccggtcat
     5041 accaaagggc atgatcatcc tgcgattgat accgactgca gtccatagca aggaggatat
     5101 cgatcagacg ctggaagcat tcagtatgat caaggatagg ttgtatagcg gtgaatatgc
     5161 aaagattgcc gctgagcgat acgtagcaaa aacctgatga agacagtata attccattac
     5221 cattctaaca gaatattata ttttgaagcc ttatatcagc ggaatacagc aaataggagt
     5281 cggggtagcc gatgtccatg aagcctggaa atggtaccat cgagcattcg gaatggatat
     5341 cccggtgttt gaagaagcgg cgactgcggc attgatgctg ccttataccg gaggtaagcc
     5401 gcaggacaga catgctatcc tggccttgaa tctccgcggt ggcggtggtt ttgaaatatg
     5461 gcaatacacc agcaggacac ctcagaaagc agctttcgaa gtgaaagccg gtgacctggg
     5521 tatctttatt gccaagatca agacttctga tatcaaaaag gcattcagcc acctgattga
     5581 ttgtggggca acaatgctgt ctgaaccggc gcaatccctc aatggcatgc gacatttcta
     5641 tgccaaagat ccatatgaca acctgtttga aatcatcgaa caaaaggatt ggttctcttc
     5701 ggggcctgga gtctttgggg ggtgttgcgg ggcagtgatt ggtgtttccg acattgaaaa
     5761 gtccaaactt tactataagg ccttgttggg ttatgaaacg atctccggag agactgaagt
     5821 aatggacaat gaatggcgga atatccccgg tggtgaaaag aaattcaaaa gagctatcct
     5881 tacacacgaa tacccgcgaa aagggccttt ttccaagcta ttgggctata cttccataga
     5941 gctgatccag gcgttggatt atcaaccgaa caagatcttt gaaaaccgat tttggggcga
     6001 cctgggatat atccaccttt gctttgatat ccaaggtatg gatgagatga gaaaactgtg
     6061 cgaacagttt ggccatccat tcaccgtgga cagtagcaat agttttgata tgggagaggc
     6121 cgcaggtcat ttttcttaca tcgaggaccc ggatggcaca ctgatagaat ttgtcgaaac
     6181 ccacaaaata ccgatcatca agaagatagg ctggtatctt aacctaagaa acagacccgc
     6241 agacaaagca ttgcccaatt ggatgcttaa ggcgctgcga ttttcgagaa gggagttgta
     6301 agagggttgg ttccggcatg tagttactat atcctgttag tttagcattc atcatcaatc
     6361 cggatgaaat ctacggctat tgaaggcaga gtttcaaaat cgcgcatggc attgatgagt
     6421 ttgaaacatt cgtaactacg aaggtcatcg acacttatat cccgggctat gacttgccca
     6481 gaagcaagca ggcgttgccg ggcggtcccg caaagtaaag ggccggcagg ggtatgccac
     6541 tcttctccat tgaacaacac tatgttgcag taagatgaat ctgtgatcaa gccgtggcgt
     6601 atgattagga tgtcatcaca gtctttcttt tgtttgaata acctgttcag gtggcttcta
     6661 tccgtgtatt tcaaggagta gtcgatatca ttggcttcga tgaggcgcag ggaagtaatg
     6721 tccctgatgg tgtatggttc ccatgtggcc ttgcgggagt tatgattata gctgattcgc
     6781 agcttgaagg tgcctgcatg acaagcttcc gggataatca ggccatcaaa aagcccgttg
     6841 gccggctcat atccgtagta ggttgtgtac gagtcgagat atcgcatcag gtgccatgac
     6901 ggatgcatga tgatgctgtt ttcgacccgg atggattcaa agagtgggta catagatttt
     6961 ctgaatcatt tcaaggtatt catcttcttc gttgctctgt gccgttatgc ctccaccact
     7021 tctaaacaac aagccatcga ttccctgttc gatatatcgg atcgccacgg cactgtccag
     7081 gttatgtcca tcaaaatagc cgaagacgcc cgtatagtaa ccgcggggac ccaattcggc
     7141 tgtccggatt atatccaggg ttttttgctt gggtgcaccg ctgacggaac ctgcaggaag
     7201 aaggagggtc aacatttccc caagccttgt acgccaattg tgtttcaacc gaccggctat
     7261 ttcggaactg gtctgaagaa tctcgccttt gtacgtcttg attctttgga caaaacggta
     7321 ttttatcacc cggatatcat gcgcgaccat agccagatca tttcgcatca gatccacgat
     7381 ggtgtt
//
LOCUS       DAOQMC010000116         7374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4102_length_7374_cov_4.820604, whole genome shotgun sequence.
ACCESSION   DAOQMC010000116 DAOQMC010000000
VERSION     DAOQMC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_7374_cov_4.820604"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2197)
                     /locus_tag="PKV31_05885"
     CDS             complement(<1..2197)
                     /locus_tag="PKV31_05885"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB92418.1"
                     /translation="MRFLISFWFFILLATIADAQVIRNNPNSNHANKFEQLGTILPTP
                     NEYRSASGSPGPKYWQQRADYNIKCTLDEGKQHLTGTEVITYYNESPDPLPYLWMQLD
                     ENQHSNINNAGYQSESEMPRQLTDRDLRNYEANKKDNGFGFNITRVVDAMGKPISFTI
                     NKTMMRLNLSKPLKPGEKIVFTIDWNYNLVNRREQGGRGGYEYFPNDDNYLFTMTQWY
                     PRMCVYSDFQGWQNHQFSGRAEFALTFGDFHVEMTVPSDHVVCATGECQNYDKVLTPA
                     QLERWKKAQTASDVMEIVTLDEAKAAEKKKSRTSKTYIFEAKNVRDFAWGSSRKFVWD
                     ALPVDIEGKKVMAMSFYGKEAYGLYRPFSTKVVAHTIRTYSHFTIPYPYPVAQSVEAS
                     NGMEYPMICFNYGRTEEDGTYSEGIKNGMIGVIIHEVGHNFFPMIINSDERQWTWMDE
                     GLNTFVEYLTEELWDNKFPSKRGPASSIVNYMKLPKDELEPIMTNSDNIVSFGPNAYA
                     KPATGLNILRETIMGRELFDHAFRNYCRKWAFKHPTPADFFRSMEDASGEDLDWFWRG
                     WFYSTDACDISLDTVKYAVADVNATVKTSRPMKRSVQGPLLNDFDDISKVRNREDKNI
                     VFETDRDTTLRDFYWKYARNLETQDTTKFNVLWPESVEPLDESLKKQFEKFHAYELTL
                     SNKGGLVMPVIIEWTYTDGSKEVERISAQIWRKNENKITILYLPPPHLPN"
     gene            complement(2406..4274)
                     /gene="mnmG"
                     /locus_tag="PKV31_05890"
     CDS             complement(2406..4274)
                     /gene="mnmG"
                     /locus_tag="PKV31_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNB92419.1"
                     /translation="MFPEYDVIVVGAGHAGCEAAVGAANMGCRVMLITMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVTDHTMIQFRMLNLSKGPAMWSPRAQSDRMAF
                     AAKWREMLESNPLIDFWQEMVSGLIVKDKRVAGVRTQMGIEIHGKAVVLTNGTFLNGI
                     IHIGEKQFGGGRAGERAARGITEQLVELGFESGRMKTGTPPRIDGRSLHYDRMDIQPG
                     DDNPGRFSFTDTPQVRDQMPCHITYTNDKVHDILRTGFSKSPMFQGRIQGLGPRYCPS
                     IEDKIERFSDKERHQLFVEPEGRNTIEIYVNGFSSSLPEDVQYKALKLIPGFEDVRMF
                     RPGYAIEYDYFPPTQLKMSLETHLIHGLFFAGQINGTTGYEEAACQGLMAGINAARYI
                     RQEDPFVLTRSQAYIGVLIDDLINKGTNEPYRMFTSRAEFRILLRQDNADLRLTPLAS
                     GLGMAGLEQRMNRVRMKNSALEEIREWFRVISGDPDNLNPYLDSVNSAPLKQKIKLDQ
                     IITRPDVKIVGLAAHFPELKEFLDRYDPEIISLAETHMKYEAYIEKEMEMVHRVNRLE
                     EMKLSPDLNYSTISSLSLEAREKLNKHKPATLGQAGRISGINPADISVLLIHLKPG"
     gene            complement(4492..5175)
                     /locus_tag="PKV31_05895"
     CDS             complement(4492..5175)
                     /locus_tag="PKV31_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNB92420.1"
                     /translation="MGSRTLEEREALEYNTDRDNLVLPEYGRYLQKLANSIKKIEDPE
                     EQRAYIEKSIDIIIQLFPESRSVDNYREKLWHDFYYMTDFQLQALPPSGIRPTFEDQQ
                     LRPEKVDYPVHKLDYKHYGYNVQVMIKKALKMEDGPVKTEFVEVIGAYMKLAYKTWNK
                     EHFVSDEMIRQDLFNMTRGALAIDEEKNLDVLVSSQIKKRFLPSSNQPQNQNSKKKKK
                     NQNKNKSRR"
     gene            complement(5205..7040)
                     /gene="glmS"
                     /locus_tag="PKV31_05900"
     CDS             complement(5205..7040)
                     /gene="glmS"
                     /locus_tag="PKV31_05900"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNB92421.1"
                     /translation="MCGIVGYIGHRDAYPIIIKGLKRLEYRGYDSAGVALMNGDLNIY
                     KIKGKVANLEAFAEGKNKSGHVGMGHTRWATHGKPDDINAHPHMSGNARLALIHNGII
                     ENYATLKEALIKLGHSFVSQTDTEVLVHLIEEIQVSENLELDEAVRIALSKVIGAYAI
                     VVMDRTNPDVLVGARKGSPLVVGLGEDEFFIGSDATPIIDYTKKVIYLQDEEIAVIKN
                     GQLQIKDMKNEIKDPKVELLDMQIEALEKGGYDYFMLKEIHQQPETVLDCMRGRFNAE
                     ESWIHLRGVEEYEEKFRNVKKLTIVSCGTSWHAGLIGEYLIEDLARLPVEVEYASEFR
                     YRNPIIGPDDIVIALSQSGETADTLAALELSKDLWALTYGIVNVVGSSIARVTHAGSY
                     IHAGPEIGVASTKAFTGQVTLLILMALKFATMRGTISKSRFQELVIELEKIPSKIEEV
                     LKKNEQIKYIAGEIKDSTNAIYLGRGVNFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDEKMPVIVIATNRAAYDKIVSNVQEVKARKGIVIAVVNEGDVEISKLADYTIE
                     IPETEDALSPLLSVIPLQLLSYHIALMRGCNVDQPRNLAKSVTVE"
     gene            complement(7073..>7374)
                     /locus_tag="PKV31_05905"
     CDS             complement(7073..>7374)
                     /locus_tag="PKV31_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92422.1"
                     /translation="KKGSDGQLTSIVDGTIAISSGYSSSFGGSLEEFTIDQKTVFKYR
                     FRLPKHLTDYIDGKEGKELYISALGAASEPGKVIINGPQSSVRPMKIKLIITKVN"
BASE COUNT         1967 a         1724 c         1400 g         2283 t
ORIGIN      
        1 tgttagggag atgaggagga gggaggtaaa gaatggtaat tttattttca tttttccgcc
       61 atatctgggc tgaaatgcgt tcgacttctt tacttccatc cgtataagtc cactctatga
      121 tgacaggcat gaccaagcct cccttgttgg acaaagtcaa ttcataggca tgaaactttt
      181 caaactgctt cttcaaactc tcatccaaag gttcaacact ttccggccag agcacattaa
      241 acttcgttgt atcttgagtt tccagattac gggcgtattt ccaataaaag tctcttaagg
      301 tcgtatcccg atctgtttcg aaaactatgt ttttatcttc ccgattcctc actttagata
      361 tatcatcaaa atcattgagc aatggaccct gcactgatcg cttcataggc ctggatgtct
      421 taacagtggc attaacatcg gcaacagcat acttcaccgt atccaatgag atatcgcatg
      481 catcggtact ataaaaccag cctcgccaga accaatcaag atcttcgcca ctggcatctt
      541 ccatcgacct gaaaaaatct gccggtgttg gatgcttgaa tgcccattta cgacaatagt
      601 tccgaaaggc atgatcaaac aattcccgcc ccataatggt ttctctcagg atattcaacc
      661 ctgtagccgg ttttgcatag gcatttggac caaaggacac tatattgtca gaattggtca
      721 tgatgggttc cagctcgtcc ttcggcaatt tcatatagtt aacgatgctg gaagcaggtc
      781 cgcgtttgga tggaaatttg ttatcccata attcttcagt gagatattca acaaaagtat
      841 ttaatccttc atccatccaa gtccattggc gctcatcgct attgattatc atggggaaga
      901 aattatggcc tacttcatgg ataatgacac caatcatccc attttttatc ccttccgaat
      961 aagttccatc ttcttcagta cgcccatagt tgaagcatat catcggatat tccataccgt
     1021 tgctggcttc aacactttga gcaaccggat aagggtaagg aatcgtaaaa tgggaataag
     1081 tgcgaatggt atgcgccact accttggttg aaaaaggtcg atataagcca taggcttcct
     1141 taccatagaa actcattgcc attacctttt ttccctctat atctaccggg agggcatccc
     1201 aaacaaactt tctggatgaa ccccatgcaa aatcccggac attcttagcc tcaaagatgt
     1261 atgtcttgga tgttctggat ttcttcttct ctgcggcttt agcttcgtcc aatgtaacaa
     1321 tctccatcac atcactggca gtctgggctt tcttccaacg ttcaagttgt gctggagtaa
     1381 ggaccttgtc ataattttga cattccccgg ttgcgcaaac aacatgatct gatgggacag
     1441 tcatttccac atgaaaatcc ccgaaggtta gtgcaaattc tgctcttccg gaaaactggt
     1501 gattttgcca cccctgaaag tcactataaa cgcacattct gggataccat tgtgtcatgg
     1561 taaataaata attatcatca ttaggaaaat attcataacc accacgccca ccttgctctc
     1621 ttcggttgac aagattgtaa ttccaatcta tagtaaaaac tattttctct cccggtttaa
     1681 gtggctttga aagattgaga cgcatcatgg tcttattgat ggtaaaggat attggttttc
     1741 ccatagcatc caccactctt gtgatattga agccaaaacc attatctttt ttattcgcct
     1801 cataatttct tagatccctg tctgtaagct gcctaggcat ttcgctttca ctttgataac
     1861 ccgcattgtt aatgttgcta tgctgatttt catccaactg catccaaaga tatggcaaag
     1921 gatccggtga ttcattatag tacgtaatta cttccgtgcc cgtcagatgt tgtttgccct
     1981 catccaatgt acacttgata ttataatcag ctctttgctg ccaatacttt gggcccgggc
     2041 ttccactggc agaccggtat tcattgggag ttggcagaat agtgcctaat tgttcgaatt
     2101 tattagcatg attagaattc ggattatttc ggataacctg agcatcagct atcgtggcaa
     2161 gtaaaataaa aaaccaaaag gatataagaa agcgcattat aaattattta attatttgaa
     2221 taccccaaat ataattttta atgtacaaaa cggtagaaat tttctactaa attaaacttt
     2281 cattatatga attacttgaa tctctgtgca ttttgttata caacaagcta taccacgatg
     2341 attcttgact gcttgagtca ttataaatcg agggtatatg agagtaattt taaaacaaaa
     2401 aggtatcagc ccggtttcag atgaatcaac aagactgaaa tatctgccgg attgatgccg
     2461 ctaatccgtc ctgcctggcc gagggtggca ggtttgtgct tattgagttt ttcccttgct
     2521 tcaagcgaaa gggatgatat ggtggaatag ttcaagtccg ggctcagctt catttcttca
     2581 agtcggttga ctcgatgcac catttccatt tctttttcga tataggcctc atatttcatg
     2641 tgcgtctcag caagacttat tatctcggga tcatacctgt cgagaaattc cttaagctcc
     2701 gggaagtgtg ccgccaatcc aactatcttt acatcaggcc gggtgatgat ctggtcaagt
     2761 ttgatttttt gtttcaatgg tgccgaattc accgaatcga ggtaaggatt caaattgtcc
     2821 ggatcaccac tgattacacg gaaccattct ctgatttctt ccagtgcact atttttcatc
     2881 ctcaccctgt tcatcctttg ttcaagtccg gccattccca atccagaagc gagtggagta
     2941 agtctcaggt cggcattatc ctggcgaagc agaatcctga attctgctct ggaagtaaac
     3001 atcctgtatg gttcattggt gcctttattg atcaggtcat cgatcaggac accgatatag
     3061 gcttgcgatc tggtcaggac aaatggatct tcctgacgaa tgtagcgtgc ggcgttgatt
     3121 ccagccataa ggccttggca ggcggcttct tcgtagcctg tagttccatt gatctggccg
     3181 gcgaaaaaca agccatggat cagatgtgtt tccaatgaca tcttaagctg ggtcggcgga
     3241 aaataatcgt attcgatggc gtagccagga cggaacatgc ggacatcttc aaaacccggt
     3301 atcaacttca atgccttata ttgtacgtct tcaggcagag aacttgaaaa tccgttaaca
     3361 taaatttcaa ttgtattcct gccctccggc tcaacaaaca actgatgtct ttccttatct
     3421 gaaaagcgtt ctatcttatc ctcgatggag ggacaatatc ttggaccaag gccttgtatt
     3481 cgaccttgaa acatgggtga tttactgaaa cctgtccgta gaatatcatg cactttgtca
     3541 ttggtgtagg taatgtgaca aggcatttga tccctgacct gaggggtatc tgtaaatgaa
     3601 aacctcccgg ggttatcatc acccggctgt atatccattc tgtcgtaatg tagactccga
     3661 ccatcaatcc tgggtggtgt tccggttttc attctaccgg actcaaaacc cagctcgacc
     3721 aattgttcgg ttattcccct ggcagctctt tcgccagctc tacctccacc aaattgtttc
     3781 tcgcctatat gtataatacc gttcaggaag gttccattgg tcaagaccac ggccttccca
     3841 tgaatctcaa tacccatctg tgtacggaca ccagcgacac gcttatcctt aactatcaac
     3901 ccagacacca tttcctgcca aaaatcgata aggggattag attcaagcat ctctctccat
     3961 ttcgctgcaa aagccatacg gtcactttgt gctcgtgggc tccacatcgc aggaccttta
     4021 gacaggttga gcatcctaaa ctgaatcatg gtatggtcag taacaatacc cgaataccca
     4081 ccgagggcat caatctctct aactatctga cctttagcta cgcctcccat agccggattg
     4141 caggacatct gcgctatagt ctgcatgttc atggttataa gcattacccg acagcccata
     4201 ttggctgcgc cgactgcagc ttcacaaccg gcatgaccgg caccgaccac tattacatca
     4261 tattcgggaa acatactttt atatagaacc taccacttga aaaaatcgaa agacaaaaac
     4321 atttaccttt tagtttagag aaatttatac gttgataacc cttaaatatt aattcattct
     4381 caggcgtttt ttgtgaaagc catgtatcta gtcagaatgt ctaatgtcag agggtagaga
     4441 ttgattcaat aaggcaatca tttaccatca atccaaatca ggtcctttac cttacctcct
     4501 gcttttattc ttgttctgat ttttcttttt cttcttacta ttttgatttt gaggctggtt
     4561 gctggaaggg aggaatctct ttttgatctg agatgaaact aaaacatcca gattcttctc
     4621 ttcatcaatg gcaagggctc ctctcgtcat gttgaaaaga tcctgacgta tcatctcatc
     4681 gctaacgaag tgttccttgt tccaggtttt gtaagccagt ttcatatagg caccaatcac
     4741 ttcgacaaat tctgttttta caggtccgtc ctccattttc agagcctttt tgatcatcac
     4801 ctgaacatta tatccatagt gtttataatc aagcttatgt accggataat caactttctc
     4861 tggtctaagc tgctgatctt caaaagtagg tctgatacca ctcggaggta atgcctgcaa
     4921 ctgaaaatcc gtcatgtaat agaaatcatg ccatagtttc tcccgatagt tatccacact
     4981 tctgctttca ggaaaaagtt ggattatgat atcgatagat ttttcaatat aggcacgttg
     5041 ctcttccgga tcttcaatct ttttaatgct gttggccagt ttttgaagat acctgccata
     5101 ttcaggcagg acaagattgt cgcgatcagt attatattct aaagcttctc tttcttccaa
     5161 tgttcttgag cccatatatt caatttaggc attaggttaa aaaattattc aacagtaact
     5221 gactttgcca gatttctggg ttgatccaca ttacatccgc gcatcaaggc gatgtgatag
     5281 gatagcaact gtaacggaat gaccgacaac agaggggata atgcatcttc ggtttcagga
     5341 atttcgatgg tataatctgc cagttttgat atctcaacat caccttcatt gactacagcg
     5401 attacaattc cctttcttgc ctttacttcc tggacattac ttactatctt atcataggct
     5461 gcacgattag ttgcgatgac gatgaccggc attttctcat cgatcagggc tattggtcca
     5521 tgcttcatct cagcagcagg atatccctct gcatgtatat aagaaatttc ctttaacttg
     5581 agggctcctt ccaatgcaac cgggaagttg actccccgtc ctagataaat tgcattggta
     5641 ctgtccttaa tttcaccggc aatgtacttt atctgttcat ttttcttaag aacctcttcg
     5701 atcttggatg gaattttttc cagttcaatc accagttcct gaaatctgga cttggagatg
     5761 gtgccacgca tggtggcaaa cttcaaagcc atgaggataa gtaaagttac ctgacctgta
     5821 aaggctttgg tagaggcaac tccaatttca ggacctgcat ggatatagga tccggcatgt
     5881 gtaactctgg caatggaaga tcccacgaca ttgactatcc cataggtcaa tgcccacaag
     5941 tccttggaca gttcgagtgc tgcaagggtg tcagctgttt ctccggattg tgacaatgca
     6001 attacgatat catcggggcc tatgattgga ttgcgatagc ggaactctga agcatattca
     6061 acttcaacgg gaagtctggc caaatcctca atcaagtatt caccaatcag gccggcatgc
     6121 caacttgttc cgcagcttac aatggttaat ttcttaacat tccggaattt ctcttcatac
     6181 tcttccacgc ctctgaggtg gatccaactt tcttccgcat taaacctacc tcgcatacag
     6241 tcaagtacag tctcaggctg ctggtggatt tcctttaaca tgaagtagtc ataaccacct
     6301 ttctcaaggg cttcaatctg catatcaaga agttcgacct taggatcttt gatttcattt
     6361 ttcatatcct tgatctggag ctggccattc ttgatgacgg cgatctcttc atcctgaagg
     6421 tatatcactt ttttagtata atcaatgata ggagtcgcat ctgagcctat aaagaattca
     6481 tcctccccaa ggcccactac cagggggctt ccttttcttg ctccgaccaa tacatccgga
     6541 ttagtacgat ccattaccac aatggcatat gccccaatca ctttggagag tgcaatcctt
     6601 acagcctcat ccaattcaag attttcagaa acctgtatct cttcaatcaa atggaccagg
     6661 acctctgtgt ctgtttggct aacgaaggaa tgaccaagct taatcaaagc ctccttcagc
     6721 gtagcataat tttcaatgat accgttgtgg attagtgcaa gccttgcatt accggacata
     6781 tgagggtgtg cattgatatc atccggctta ccgtgtgtcg cccagcgcgt atgccccatt
     6841 ccaacatggc cggatttgtt ttttccttct gcaaatgctt caagattagc gaccttgcct
     6901 tttattttat aaatattcaa atcgccattc atcaaagcta cccccgcact atcatatcca
     6961 cgatattcaa gcctttttaa ccccttaata ataattgggt atgcatccct gtgtccaata
     7021 tatccgacaa taccacacat attacaattt gcttgatttt tgtatcccga aattaattta
     7081 ctttggtgat gataagctta attttcatag gcctaacgct ggattgtggc ccgttgatta
     7141 tcacctttcc aggctccgaa gccgctccca gggcactaat atacaactct ttgccttctt
     7201 ttccatcaat ataatccgtt aaatgtttgg ggagcctaaa tctatactta aataccgtct
     7261 tttgatcaat tgtaaattct tcaaggctac caccaaatga cgaactatag ccgctactga
     7321 ttgcgatcgt gccgtccaca atagaggtaa gttgtccatc gctccctttc ttat
//
LOCUS       DAOQMC010000117         7350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4123_length_7350_cov_5.235230, whole genome shotgun sequence.
ACCESSION   DAOQMC010000117 DAOQMC010000000
VERSION     DAOQMC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4123_length_7350_cov_5.235230"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PKV31_05910"
     CDS             complement(<1..1185)
                     /locus_tag="PKV31_05910"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNB92423.1"
                     /translation="MSSNEAGFITLPEGTTSIWVSCIGYLTMAISTDTLKYTNDKTTI
                     LMEPTIMTLEEIIITSGMQQSVYKEISDLDIHMRPINNSQEVLRMVPGLFIGQHAGGG
                     KAEQLFLRGFDIDHGTDIQISVDGMPVNMVSHAHGQGYADLHFVIPELIDRVQFDKGP
                     YFGDHGNLATAGYVDFRTKNVLDRNFIKVEGGQFSTLRGLFGANLIRSGNEEKSLLMA
                     GEGYYTRGYFDSPQGFRRFNGLLKYNTRLNRHNDLNVTFSGFTSRWNASGQIPNRAVS
                     DGLIGFYGAIDDTEGGETSRYNASATLTSKFKDGSRWINQIYYNRYQFLLYSNFTFFL
                     RDSINGDQIRQAEKRNLTGINSKYSKQYQVGDFRLITTAGLQMRTDIVDDIELSHTKD
                     REI"
     gene            1524..2138
                     /locus_tag="PKV31_05915"
     CDS             1524..2138
                     /locus_tag="PKV31_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNB92424.1"
                     /translation="MQEQTVKTPILIYTEETPNPDSLKFVVNIILYPGGTGDFRDVES
                     AQKWSPLAAALFEFPYVKGVYISNNFVSVTKEATYSWVEIQQSIKDFIKHYVIDDKPV
                     INEGYGEELKRLEAERIAKAYSGDTAAIVQKIKDLIETVVKPAVANDGGNIEFKSYDK
                     GVVTVIMQGSCSGCPSSTLTLKAGIEGMLKRMVPEVLEVVSEMG"
     gene            complement(2168..3091)
                     /locus_tag="PKV31_05920"
     CDS             complement(2168..3091)
                     /locus_tag="PKV31_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HNB92425.1"
                     /translation="MSLWNSYINGFQNYLRLEKGLSAHTQAAYLSDVGKLAWHIEQTG
                     TDKSPLQVEASDILDFIIEINKMGIGERSQARILSSFRSFFRYLLIEDLIQSDPVELI
                     QGPQLPTRYPEVLTIEEIEAFLAAIDLSEPLGHRNRAMFETMYACGLRVSELIHLKLA
                     GYFPDIGFVRVIGKNNKERIVPIGEIAIKQITFYLEGERRQITPKQGFENHIFLNRRG
                     AGLTRNMVFLLTKEYAQKAGITKNISPHTFRHSFATHLVEGGADLKAVQDMLGHESIT
                     TTEIYTHLDLGYLRETIFNFHPLFSGRKNQK"
     gene            complement(3517..5115)
                     /locus_tag="PKV31_05925"
     CDS             complement(3517..5115)
                     /locus_tag="PKV31_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNB92426.1"
                     /translation="MKHVTLFPLLIITLVILLSGCAENPRQKLVPLVDEATYSEVDFT
                     DVKIDSALVSTFLATLNTTDSIKSRVASFYRRRNYQFGWFTKTGMTYPVNIFYDQLNE
                     YINDFKDSTLVDPTLDSLIQLARKDERSFLKVRTNAPKLDLLLTTYYFKYADRAYGGL
                     DKSPVDLEWYIPRKKKNYEMLLDSLVIRKKTDKIKEPENIYYTRLIKELKRYRQIEKN
                     GGFPLIEEGKEIKKGDTSDVIARIRDYLRLTGDLAEGDTGRIFDDNLSKSIKIFQSRM
                     GLIPNGKPGKSTIDAMNVPIDRRIQQIMINLERLRWMPDRLVDNMILINIPEYVLHYY
                     KNDSLAWNTNVVVGKEITKTVIFSGNMSNIVLNPYWNVPQSIMKKEIIPAMQKNPNYL
                     DKNNMEIISKSPLSVRQKPGKNNSLGKLKFLFPNNYSIYLHDTPAKYLFSQNKRAYSH
                     GCVRVENPVTLAKLLLTDQTQWLKKVDAILKTDKETWIPVKPPMPVFIVYFTTWVDQA
                     GNLNFRNDVYGHDKKLGEEIFGKD"
     gene            6114..>7350
                     /locus_tag="PKV31_05930"
     CDS             6114..>7350
                     /locus_tag="PKV31_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB92427.1"
                     /translation="MGLKDRIQSFFTGLYRSRFSNWITVLGLYMAFSLLIRIAFSIWS
                     AGDASYSLYNVSRAYGIGFAYDLAVGMSFLFGYAVYLILLPARWIGSVFDRVCTYGYL
                     SIMLFIMYFSLMAEVPFWEEFATRFNFIAVDYLIYTYEVVANINQSYPLPLLIGGMVA
                     IILGTFWLLKKTGARRNTFNAKSGFFRRFTIGLPTLLLTPILLTMLNNKQAEFSENVV
                     VNEIGKNGVFSFFAAFRSNELDYDAFYPKVAESEVYPVLRQDLKQLNQTYPLCLPDDV
                     MRFTKGDQTVKEDTPNIVLVVIESFSADFSGLFGNDLNLTPVYDSLAKQSVLFTNLFA
                     TGTRTVRGMEALTLCVPPTPGNSIVRRPDNDSLFSIGTILRDKKYVPYFIYGGDGYFD
                     NMNNFFGGQGFTIVDRNRGN"
BASE COUNT         1931 a         1522 c         1564 g         2333 t
ORIGIN      
        1 gatctccctg tctttggtat gagataattc aatatcatcg acgatatctg tgcgcatctg
       61 caatcctgcc gtagtgataa gtctaaagtc tccgacctgg tattgctttg aatacttgct
      121 gttgatccct gttagattgc gcttctctgc ttgtcggatt tggtcaccat tgattgaatc
      181 tcggaggaaa aaggtgaaat tggagtacaa caggaattga taccgattgt agtatatctg
      241 attgatccat cgacttccat ctttgaattt actggtcaat gttgcacttg cattatacct
      301 gcttgtctct ccgccctccg tgtcgtcgat agctccgtaa aatccgatca acccatcgga
      361 tacagcccgg ttgggtatct gaccgcttgc attccacctg cttgtaaagc ccgaaaaggt
      421 gacattcagg tcgttatgcc tattcagtcg ggtattgtat ttaagcagtc cgttgaatcg
      481 tctgaatccc tgtggactgt caaaatatcc tcttgtataa tatccctctc ctgccatcaa
      541 taggcttttt tcctcatttc ccgaacgaat aagattggct ccgaaaagcc ctcgtagtgt
      601 gctgaactgt ccaccttcca ctttgataaa attccggtca agcacgtttt ttgtcctgaa
      661 gtccacatag cctgcagtag ccaaattgcc atggtcgcca aagtaagggc ctttgtcgaa
      721 ctgaaccctg tctatcaatt ccggtattac gaaatgcaga tcggcatatc cctgtccgtg
      781 agcatggcta accatattga caggcatacc gtctacgctg atttgtatat ccgtaccgtg
      841 gtcgatgtcg aaacctctga ggaaaagctg ctctgccttc cccccaccgg catgttggcc
      901 aataaagagg ccgggtacca ttctcagcac ttcctgactg ttgttaatgg gtctcatatg
      961 gatatcgagg tctgaaatct ctttgtacac actttgttgc atgccggagg taatgatgat
     1021 ctcttccagg gtcattatgg taggttccat caggatggtc gtcttgtcat tggtatattt
     1081 caaggtgtcc gtactgattg ccattgtgag atagccgatg caggaaaccc agatggaagt
     1141 tgtcccttcc ggcaaagtga tgaatcctgc ctcatttgaa ctcataaaat caccggttct
     1201 gttacaattt acgacaacat ccgccacagg ccgttccgtc agcttattta agaatcggat
     1261 acttctatct tgtccggaag cctggtctga aataataaaa ctgatgcaaa aaggcagtat
     1321 tagccttgat agtaaaagct tgtaaaatac ttttttcata ccttgctaag atatatcatt
     1381 taaccttaat agacttattt ctttgacgaa gattctgatt cttcagacaa gattggtaat
     1441 tcactatttg gaggcctttt tctcttcctg caactttatt atctttgccg tgttttatta
     1501 gcctaaatgt aaatgaagga aaaatgcagg aacagacggt aaaaactcct attctcatat
     1561 atactgagga gacacccaat ccggattcac tcaaatttgt tgtaaatatc attttgtacc
     1621 cgggtggaac aggtgatttc agggatgttg aatcagctca aaaatggtcg ccacttgcag
     1681 cagcgttgtt tgaatttcct tatgtaaagg gtgtttacat ctccaataat tttgtttccg
     1741 tgaccaagga ggcaacatat agctgggtag agattcagca aagtatcaag gactttatca
     1801 agcattatgt gattgatgat aagcctgtga ttaatgaagg ttacggggag gaattgaaac
     1861 gccttgaggc ggaaaggatt gccaaagcat attcaggcga tactgcggcc atcgtccaga
     1921 aaatcaagga cctgatcgag actgtcgtga agcctgccgt ggccaatgat ggaggaaaca
     1981 tagaattcaa atcctatgat aagggtgtgg ttaccgtcat catgcagggc tcatgcagcg
     2041 ggtgcccttc ttctaccctg actctgaaag ccggtatcga aggcatgctc aagcgtatgg
     2101 ttcccgaagt actcgaagta gtcagtgaga tgggttgatg atatcttcga cctgatttat
     2161 tattatttta tttttgattt tttcgccccg aaaataatgg gtgaaaattg aatattgtct
     2221 cacggagata acccagatcc agatgggtat atatctcggt cgtggtgatg ctttcatgac
     2281 ccagcatatc ctggactgcc tttaagtctg ctccaccctc tacaagatgt gtagcgaaag
     2341 aatgcctaaa ggtgtgtggg ctgatattct ttgtgattcc tgccttttgt gcatattctt
     2401 tggtcaacag gaaaaccata tttctggtca gacctgctcc tcgcctgttg agaaaaatat
     2461 gattttcaaa accttgtttt ggtgttattt gccgcctttc tccctcgagg taaaaggtga
     2521 tttgcttgat ggcgatctca ccgatgggta caatcctttc cttgttgttt ttccctataa
     2581 cacgaacgaa cccgatatcc ggaaaatatc ctgccaactt taaatggatc aattcactga
     2641 cacgtagccc acaggcatac atggtctcaa acatagccct gttgcgatgt cccaatggct
     2701 cactcaggtc gatggctgca aggaaggcct cgatctcttc tattgtcaat acttccggat
     2761 accgggtcgg cagttgaggc ccctgaatca gctccaccgg atcagactga attaaatctt
     2821 ctattaacag gtatcgaaaa aatgaacgaa agctgcttaa tatcctcgct tgacttcgtt
     2881 cgccaatgcc cattttgttg atttcaatga taaagtcaag tatgtcgctt gcctcgacct
     2941 gcaagggcga tttgtccgtg cctgtttgtt caatatgcca tgccagcttt ccgacatccg
     3001 acagataggc tgcctgggta tgagcactaa gccctttttc gagcctcaga taattttgga
     3061 aaccattgat gtaactattc caaagggaca tagcaagtta ttctaatggc aaatgtaaca
     3121 atgaattggt aaatcaaggg tagaggaaat gtggaaagat aacggattat gattcattgg
     3181 cattgtagtg atcggcccgg aagttctttg caacataagt gtaacaagag ctgtcctcca
     3241 ccggtgaatt tcacggtttg tacacacagc aatcgaatca atccggatta atagggagtt
     3301 tttaatcttg ggaagaataa agcactataa gggaaggcag atttattgcc tgaagaccgg
     3361 gaaatctgat aagctcttta ggattgttac taaatatcga ttgatgggcg ttgtccattg
     3421 aatgattgaa aatagatttc aggatttaat aacatcctgt ccgatgacgg agttgttagg
     3481 gcaatgatag cgtatatttg gttgtacgat aaaaaattaa tctttaccaa aaatctcttc
     3541 ccccagtttt ttatcatggc catagacatc atttcgaaaa ttgagatttc ccgcttgatc
     3601 cacccatgtc gtaaaataca cgatgaatac cggcattgga ggcttgacgg gtatccaggt
     3661 ttccttatcc gtttttaata tagcatctac ctttttcaac cattgagttt ggtcggtcag
     3721 aagcaatttg gctaaagtaa cagggttttc gacgcgaacg caaccatggc tgtatgctct
     3781 tttattctga gaaaacaggt atttggccgg tgtgtcatgc agataaatac tgtaattgtt
     3841 agggaaaagg aatttcagct tacccaaaga attatttttt ccgggctttt gtctgactga
     3901 caatggtgat tttgatatga tttccatatt gtttttgtcc aaataatttg gattcttctg
     3961 catagccggg atgatttcct ttttcatgat gctttggggt acattccagt atggattcag
     4021 tacgatgttg gacatattgc cggaaaagat tactgttttg gtgatttcct tacctaccac
     4081 cacattggta ttccaggcca gggaatcatt tttataataa tgcaaaacat attcagggat
     4141 attaatcagg atcatgttat cgacaagtct gtcaggcatc cacctgagcc gctccagatt
     4201 aatcatgatc tgttggatgc gcctatctat aggtacattc atcgcatcaa tggtagattt
     4261 tccgggtttg ccgtttggaa taaggcccat tctggactgg aatattttaa tacttttgct
     4321 caggttgtca tcaaaaatcc taccggtatc tccttctgct aaatcaccgg taagtcgcag
     4381 ataatccctg atcctggcta ttacatctga tgtgtccccc ttttttattt cctttccttc
     4441 ctcaatcaat gggaatccac catttttctc tatctgccgg taccttttca attctttgat
     4501 cagtcgcgta taatagatgt tttccggttc cttgatcttg tcagtttttt ttctgataac
     4561 caaagagtcg agcagcattt cataattttt cttctttctc gggatatacc attccaggtc
     4621 aacaggactt ttatcaagac cgccatatgc cctgtccgca tatttgaagt aataggttgt
     4681 gagcagaagg tccagtttgg gtgcatttgt cctcaccttc aaaaaggaac gctcatcttt
     4741 ccgggccagt tgaattaagc tgtccagggt aggatcgaca agtgtggagt ccttgaaatc
     4801 attgatatat tcattcagct ggtcgtaaaa gatattcacg ggataggtca tgcctgtttt
     4861 ggtaaaccag ccaaactgat agttcctccg acgatagaag gatgctaccc ggcttttgat
     4921 cgaatcagtt gtattcaacg tggccagaaa ggtggagacc aatgcactgt cgatttttac
     4981 atcggtaaaa tctacctcgc tatatgtcgc ttcatccacg agaggaacga gtttttgcct
     5041 tggattctca gcacatccgg atagtaaaat caccaatgtg atgattaata aagggaaaag
     5101 ggtaacatgc ttcattggcg gtttctttaa agaatgttct tttaccgggt aatgataatg
     5161 aattttttgt tcttaacaat gctatctcaa ttcagatggc tttcgtacac catgtcatca
     5221 aagattcaat tttcaaagat gcctgacaac aatagaatat tcagggcaac atgatctttt
     5281 cttcaggatg tttcattcgt cttcaatatc aaaacatttt tataacaaat tgtccttatt
     5341 taattctaca tcaaaatgct gtaatgtgtt gtctggcgag cctgaaaagt gtattgtttt
     5401 ttctgtttaa tcccctgccg ggtgtggaca aatacattcc ggtaaatact tgtcaacttg
     5461 atgagaatag caatttgcat gcatgggctg atataaaaac tgtgatgaca gaatcaatgc
     5521 ccctttcatc aaacccgggc acgagatcag tcatgtgttt tgaaatattc aggccatctt
     5581 catgtatgac agattcagcc cttgacaatg cagtaagagt ttcgataccc aaacaatatt
     5641 tcacagtcct ttcgtataag catcacctgg gatagcttac ggacaagcac cttctgcatg
     5701 gattacctgt gctaagggcc aatgtcttca ttttgatggg attgaaagga ttatcaacaa
     5761 gatgcggaaa tcggataacg gcctacatct catattcttc ataaccggtg atgccgaaga
     5821 tgatattcag gtttttgttg aaaaaatcga tttcatctta cgaaggaaag caggttgatt
     5881 cttgtccatc ctataatatg tccttaatca ttagtgctaa acttttaata aagccttatc
     5941 cttgttttgg tatttatagg tctgatattc tttttgatcc ggtcaatagt tacatgtttt
     6001 attacctatg attaccttta cattcatttt cagggtatat cgacctgatt gggttctcat
     6061 atgaaaggtc ggtatgttta cggatgattt taaaataata gtaattgatc gacatgggtt
     6121 tgaaggatag aatacagtca ttttttaccg gtttgtatcg aagccgcttc tcaaattgga
     6181 taacagtatt gggattgtat atggcatttt cattgttgat acgaattgct ttttcaatct
     6241 ggtccgcagg cgatgccagc tattcccttt acaatgtgag ccgtgcctat gggataggct
     6301 ttgcgtatga tctggcagtg gggatgtcgt ttctcttcgg atatgcagta tatctgattc
     6361 tattacctgc ccgatggatt ggttctgtct ttgatagggt ctgcacctat ggttatctct
     6421 cgataatgct cttcatcatg tatttcagcc tgatggcgga agtgcctttt tgggaggaat
     6481 tcgctacgcg gttcaatttt attgctgtcg attacctgat ctacacctat gaagtagtcg
     6541 ccaatatcaa tcaatcctat ccacttcccc tgctcattgg gggaatggta gccattatcc
     6601 tggggacttt ttggctgttg aaaaaaacag gtgccaggag aaatacattc aatgcaaagt
     6661 ccggcttttt caggcgattc accatcggac tgccaacgct tttactgaca ccaatcctgt
     6721 tgacgatgct caacaataaa caagctgagt tttccgaaaa tgtcgtcgtc aatgaaatcg
     6781 gtaagaatgg cgtattctcc ttctttgcag ctttccggtc caatgagctt gattatgatg
     6841 cattctatcc caaagtagca gaatcagagg tgtatcctgt tcttcggcaa gatctgaagc
     6901 agcttaatca gacctatcca ctgtgtcttc ccgatgatgt catgcgattt actaagggcg
     6961 atcagactgt caaggaagac acgcccaata ttgttcttgt agtgattgag agcttcagtg
     7021 ctgatttttc cggtcttttt ggcaatgatt tgaatcttac gcctgtgtac gacagtttag
     7081 ctaaacagag cgttttgttt accaatcttt ttgcaacagg tacacggact gtacgaggca
     7141 tggaggccct gactctgtgt gttccgccta caccaggcaa cagtattgtg agacgcccgg
     7201 acaatgatag cctgttctcc atcggtacga tattgcggga taagaagtat gttccctatt
     7261 ttatttatgg cggggatggt tattttgaca atatgaataa tttcttcggt ggccagggat
     7321 ttactattgt tgataggaat cggggcaatc
//
LOCUS       DAOQMC010000118         7306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4173_length_7306_cov_4.363674, whole genome shotgun sequence.
ACCESSION   DAOQMC010000118 DAOQMC010000000
VERSION     DAOQMC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_7306_cov_4.363674"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..719)
                     /locus_tag="PKV31_05935"
     CDS             complement(345..719)
                     /locus_tag="PKV31_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNB92428.1"
                     /translation="MSIGIMIAKTLVAIVAIQHLFFMWLEMFAWETVGRKFFKGNRQG
                     DFFRQTKSIAANQGLYNGFLAAGLIWSFLISDSLWQINVMTFFLGCVVVAGIFGGYSV
                     SRKIFLVQAIPAMLALIFLHLL"
     gene            1207..2238
                     /gene="obgE"
                     /locus_tag="PKV31_05940"
     CDS             1207..2238
                     /gene="obgE"
                     /locus_tag="PKV31_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNB92429.1"
                     /translation="MADQNFIDYVKICCRSGKGGAGSHHYFRDKMNSKGGPDGGDGGR
                     GGHIILKGNRNVWTLINLKYRKHVIAEDGVNGSKNNRTGRSGKDVILEVPLGTVAKSA
                     ETGEILFEITQDKQKRILTPGGRGGLGNAHFATAAKQVPKYAQPGEPGLEEWNILELK
                     LLADVGLVGFPNAGKSTLLSSVSAAKPEIADYPFTTIVPNLGLVRHRDNTSFVMADIP
                     GIIEDAHLGKGLGIRFLRHIERNSLLLFLVPSTTEDIGREYHILLNELRLYNPELLDK
                     PRLLAVSKSDLIDDELKSWLMPTLPEDVETIFISAVTNEGLDALKDKIWKYLNEDIPP
                     QDHDFSEEE"
     gene            2235..3257
                     /locus_tag="PKV31_05945"
     CDS             2235..3257
                     /locus_tag="PKV31_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNB92430.1"
                     /translation="MTEQLIAVREFAREYFNLDPDRVTQMAGAGSSRQYFRLLFIGKP
                     VIGTWSEDIKENEAFFYLSEILYSAGINVPQVIAIDESRKIYLQTECATYDAYSILTG
                     ASYKDQQPWLRKIIDQLCRIQTFVDPLVDYSRCYPSSFFGKEDVMTDLTYFERYFLEP
                     IGIRFDQNMLKQEFEKMAQRVENSHFMGFMYRDFQSRNIMVREDGELCFIDFQGARKG
                     PCTYDVISLLYQAKLGLNTVQREELLDYYYSGILSACRIELSDLKADKDVVLPVRLLQ
                     VLGAYGRRGIQERKPHFVTSIGPAIDNLSEMPDSQDFTSNYPYLTSIIQEIIGSKESI
                     LCQIKD"
     gene            3239..3649
                     /locus_tag="PKV31_05950"
     CDS             3239..3649
                     /locus_tag="PKV31_05950"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNB92431.1"
                     /translation="MSDKGLNILVRSFSYKKRSLEDSSGHGIGFVFDMRGIDNPGRIE
                     SMKILTGKDEEVRNYLEMETNMPEFISHIKGLLDITVKNYMQRNFTDLTVNFGCTGGQ
                     HRSVYAAETIAAYLNEKFPVQVTLQHLEKESWIR"
     gene            complement(3891..4595)
                     /locus_tag="PKV31_05955"
     CDS             complement(3891..4595)
                     /locus_tag="PKV31_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB92432.1"
                     /translation="MKKLHYFLLFSLFIVNSNQLSSQEVMSREKSMVNNNEFRRSYLS
                     TGYEGMIFSSSIFERPGKASHLTTLRFTLFFHFGTMYNYNFNSNLGMMTGLSIKNIGF
                     IDKYEAQDSTVKRRVYSLNLPVALKLGRMDSKRFFFIGAEGSLALNYKEKGYVKRNHK
                     TKFNEWFSDRTPLIMPNVFLGYQHKNAYLKLSYYPMNFLNPDFVDKNNVKPYQDYKVN
                     LFAVTIGTNVVSRRFK"
     gene            complement(4755..>7306)
                     /locus_tag="PKV31_05960"
     CDS             complement(4755..>7306)
                     /locus_tag="PKV31_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB92433.1"
                     /translation="NNEKNKVIVAILYANRDYYLDVLNQSINDSGVLTFDHLILNLHR
                     SIVAGNNKKLKELIRQKYRVAFIDEFQDTDVIQFQLFEKLFIDDQYDDDTTLFLIGDP
                     KQSIYSFRDADVESYLHAKSKVDHIYTMNTNFRSSTRMIEAANEFFKASGDNVFGYEA
                     EKETGIYIGYTEIQANKHNKKFLRRQEEVEETLLFCNANDAATSQTQLVETVKYLLNP
                     EEQIQLADKEDNQLRAVRPRDIAVLTRDKYSAKAIKEKLHALGIPAVSVDDTLVTKST
                     EAKEMGLVLQAMLKPDIKNIRSALFLTFLNTVYKNLTAENLPVLFIDEIKAIEKFSHY
                     HEIMLYQGFYQAFMILMEDFSVEENLSLNYASQRSLANILQLSEILHDLQYRKGLDPE
                     ELMDWLRRADSQDSEEGEYTLRIESDEEAVSIMTLHKSKGLEFPIVLIEGLKDSALKD
                     DQLVKVKPGGHAGNNDCIRTLRHPLDLDQEGLDLWKANDRREIRRLIYVGITRAEYQC
                     YVFYSTHWSTGFNTSPLKEMMENLGESTRVKKGFVPPFTDYAYKDILPDKKELTIKSF
                     NPADIEPANSAWRIFSYTALRQPHSFAPAFQEHAGSDYDHFVFNLLPRGANVGTKLHD
                     LFENIDFQTEFGTGANLSDYERRLLSLFNRKKANGEEEDYSEYIAQMLEHTLNAKIGV
                     DGSMILLKNVLSGKRIHEMEFDFCLNQENIKPNLTPLLSEYDINLSPDLTQLQGIMNG
                     FIDLLFEHEGRYFILDWKSNHLGYRLENYESPGLNEAMKDNQYKLQYLIYSIAVHRFL
                     KQRLRDSYDYDVHFGGVIYVFLRGVRAGAATGIYFDRPEVGFVDKLDKIFG"
BASE COUNT         2250 a         1529 c         1400 g         2127 t
ORIGIN      
        1 tagtccataa atttttggta tacttgtccg gatagctatg ctactagagc tctgccctgt
       61 cccgggagtg caacaatccg gggttccggg atgccgaggt tacagtcgta tagatcaatg
      121 aaattggtcg atagcgaatg taccttcggt attatattta ggattgacca aagagaaaca
      181 tttccagatt ctttcaatct aaatacttta tgaaaccggg caataattta taattctcct
      241 gtgtattatt tagattggct gattgaattt gcactatgaa gagtttccac gggacttcat
      301 gcggcaataa caggagaatt tttccttgaa taacgaaaaa acattcataa caaatgcaaa
      361 aatatcaagg ccagcattgc cggtattgcc tgcacgagaa aaattttccg tgaaactgaa
      421 taaccgccaa atatccctgc aacgactaca catccaagaa aaaacgtcat aacattaatc
      481 tgccaaaggg aatccgatat cagaaaactc cagattagac cggctgctaa aaacccgtta
      541 tataacccct gatttgccgc tatgcttttt gtctgtctga aaaaatcacc ctgcctattt
      601 cctttgaaga atttccgccc aactgtttcc cacgcaaaca tttcaagcca catgaaaaat
      661 aaatgctgga ttgctactat tgcaacaagt gttttcgcta tcatgatgcc aatgctcata
      721 cttaaaaaat ttaggttttt cacgttatcc tgatggtgca actcacggat ttcgagtaac
      781 tatccacaag caatgccaac tgttatctca aaagtatcaa ctaggagcag acactgcaat
      841 ccctgggctt ctgtcggttc aactatgcta atatgatacc ggtcaggtcg ccaaagttga
      901 aacaagatac acaatgttta tggtatacaa tcgattaaat taattatctg cataaatgta
      961 cttttgagag cgcccaagta gtctttaaca ctttacagaa ttaattttcg gacactcaaa
     1021 ttttaataat aagatatatt aatataatat ataatacact tactatctat atattataac
     1081 acaaacacat agcttttatc atttattcac cccgtaatac ttttcaatag aagcaaagaa
     1141 gaatttgtga tactcatctc tcaccccttg atgctgtcca attatttttt gcatctttgc
     1201 agcacaatgg cagatcagaa tttcatcgac tatgttaaga tatgttgccg ttccggtaaa
     1261 ggcggagcgg gttcgcatca ctactttagg gataaaatga actcaaaagg tggtccggat
     1321 ggcggagatg gaggtcgagg cggacatatt attctgaaag ggaatagaaa cgtatggaca
     1381 ttgatcaacc ttaaataccg aaagcatgtc attgccgagg acggtgtcaa cggatctaaa
     1441 aacaaccgca ccggccgcag cgggaaggat gtcattttag aagttcctct tggcacagta
     1501 gctaaaagcg ctgaaaccgg agaaatttta tttgaaatca cgcaagataa gcagaagcgc
     1561 atcctgactc cgggcggcag aggtggcctt ggaaatgcac actttgctac ggcggcaaaa
     1621 caggtgccga aatatgcaca acccggagaa ccaggacttg aagaatggaa cattctggaa
     1681 cttaaacttt tggctgacgt aggcttagtt ggatttccaa atgcaggcaa atccactttg
     1741 ctatcatcag taagtgcagc aaaacctgaa atagcagatt atccattcac taccatcgtg
     1801 cccaatctgg gtctcgtaag gcatcgcgat aatacctctt tcgtcatggc cgatatcccg
     1861 ggaatcatcg aagatgctca tctcggaaaa ggcctcggta tccgcttcct gcgccatatt
     1921 gaaaggaaca gccttttgct cttccttgta ccttccacaa ccgaagacat aggcagggaa
     1981 tatcatatcc tgctgaatga attaagactt tataatcctg aacttctgga caaaccgcgc
     2041 ctgctggcag ttagcaagtc ggatctgatc gatgatgaac tgaaaagttg gcttatgcct
     2101 accttaccgg aagatgtgga gacgatattc atttccgccg taaccaacga aggtcttgac
     2161 gcgctgaaag acaagatttg gaagtacctg aatgaggaca ttcctccaca ggatcacgat
     2221 ttttctgagg aagaatgacg gaacaactta tagcagtaag ggaatttgcc agggagtatt
     2281 ttaatctgga ccctgatcgc gtgactcaaa tggcaggtgc aggaagcagt cgccaatact
     2341 ttagattgct tttcattgga aagccggtta taggtacctg gtcagaggac atcaaggaaa
     2401 acgaggcatt tttctatcta tcggaaatct tatactccgc aggaatcaat gtccctcagg
     2461 taattgcaat tgatgaaagt cgaaaaatat acttacagac agaatgcgcg acatatgacg
     2521 cctacagcat ccttacagga gctagctata aggatcaaca gccgtggctc agaaagatca
     2581 tcgaccagct ttgtagaatc caaacctttg ttgatccttt ggtcgactac tccagatgtt
     2641 atccttcttc attttttggc aaggaggatg tgatgactga tctgacctat tttgaaagat
     2701 attttctgga accgatcggc atccggtttg atcaaaatat gttgaaacaa gaattcgaaa
     2761 aaatggctca acgtgtcgaa aacagccatt tcatgggatt catgtaccgg gattttcaat
     2821 cgaggaatat catggttcgt gaagatgggg agctgtgttt tattgatttt caaggggcac
     2881 gtaaaggacc ttgtacctat gatgtcattt ccttattgta tcaggctaaa ttaggactga
     2941 atacagtaca gagggaggag ttattggatt attattattc cgggatactt tcagcatgca
     3001 ggattgagct ttcagatctt aaggcagata aggatgtagt actgcctgtc aggctcctgc
     3061 aagtactcgg tgcatatggc cggcgtggga ttcaggagcg aaagcctcat tttgtgacca
     3121 gtattggccc tgccattgac aacctcagcg aaatgccgga ttcgcaagac tttacttcaa
     3181 attacccata tctaacttca ataatacaag agattatcgg atcaaaagaa tcgatattat
     3241 gtcagataaa ggattaaata tactagtaag gagtttttcc tacaaaaaaa ggagccttga
     3301 agattcatca ggtcatggca tcggatttgt attcgatatg cgtggcattg ataatccggg
     3361 aagaattgag tcgatgaaaa tactgacagg taaagacgaa gaggtaagaa attatctgga
     3421 gatggaaacc aacatgcctg aatttatcag ccatatcaaa ggattgctcg acatcacagt
     3481 aaaaaattac atgcaacgca atttcactga tctaacagtc aattttggat gtacaggggg
     3541 acaacaccgc agtgtttatg ccgctgagac catagctgcc tatttaaatg aaaaatttcc
     3601 ggtacaagtg acgttacagc acctcgagaa agaaagttgg atcagataaa ggaagattca
     3661 tccttaagaa tgaataggcg ataactatga tgattggctt gcagattttc cggaatcaaa
     3721 ttctgtcata ccaatcgtat aaacatcttc atgctcccct ggtttcggtt taatcgacaa
     3781 aagcttaatg cattgtccct gaacaaacag gtaatcgtca gacacaatga tgtgtttttc
     3841 gcaggacaat tgaaaaaata ttctgatcaa aattgaccta ctgcgccagg ttatttaaac
     3901 ctgcgggaaa cgacatttgt gccgatcgta actgcaaaga gattgacctt atagtcttga
     3961 tacggtttca cattattctt atcaacaaaa tccggattca ggaaattcat cggataatag
     4021 ctcaatttta aatacgcgtt tttgtgctga taccctaaga atacattggg cataatcaat
     4081 ggcgtcctgt cactaaacca ttcattgaac ttagttttat ggtttcgttt aacatatcct
     4141 ttttctttgt aatttaaagc aagtgaacct tctgcgccga taaagaaaaa ccgcttcgaa
     4201 tccatcctac ccaacttcaa cgcaaccgga agattcagag aatagactct tctcttgacc
     4261 gtactgtcct gcgcttcata cttatcaatg aagccgatat tcttaatact caagccagtc
     4321 atcatcccaa ggttcgaatt gaaattatag ttgtacatag tcccaaaatg gaagaacaat
     4381 gtaaaacgaa gtgtggtcaa atgagaggcc tttccgggcc tttcgaaaat tgacgaactg
     4441 aagatcattc cctcataccc tgtagaaaga taacttctcc taaactcatt attgttaacc
     4501 attgattttt cacggctcat cacctcctgt gaacttaatt gattggaatt cacaataaaa
     4561 agcgaaaaca gaagaaaata atgaagcttt ttcatcgatg catgattgat tttattaaaa
     4621 aaataaagtg tgcctcaaat ttagtaaaat catcaatcaa aattgtataa aataatgaaa
     4681 atttcaaaac tatctgttcc gggttacaac ccaatctgct tcgaatgcac tcacaaaatt
     4741 ataattccgg accctcatcc gaatatcttg tccaatttat ccacaaatcc aacttccggc
     4801 ctgtcaaaat aaattccggt cgctgctcct gcccgcacac cccgcaaaaa gacataaatt
     4861 acccccccaa aatgcacatc ataatcatat gaatctctta atctttgctt caggaaacga
     4921 tgtacagcaa tagaatagat caaatactgc agtttgtact gattgtcctt catagcctca
     4981 ttaagtccgg ggctttcata attttctaat cgatagccca ggtggttgga tttccagtcc
     5041 aggataaaat aacggccttc atgttcgaaa agcaggtcga tgaaaccgtt cataatgcct
     5101 tgcagttgag tcagatcagg gcttaaatta atatcatact ccgacaacaa aggtgtcaga
     5161 ttaggtttga tattctcctg attcaaacag aagtcaaatt ccatttcgtg gattctcttt
     5221 cccgacaata catttttgag taaaatcatt gatccatcta cgcctatttt tgcattcaag
     5281 gtatgttcaa gcatttgggc aatatactcg ctataatctt cttcctcgcc gttggctttc
     5341 tttcgattga acaaactcaa tagtcttctt tcatagtcag acaagtttgc cccggttcca
     5401 aattccgtct gaaagtcaat attttcaaat aaatcatgga gctttgtgcc tacattggca
     5461 ccacgtggca gaagattgaa aacaaaatga tcataatcgg agccagcatg ttcttgaaag
     5521 gcaggggcga agctgtgggg ttgacgaagg gctgtgtagc tgaaaattcg ccaggcggag
     5581 tttgccggtt caatatcggc aggattgaaa gatttgattg tcaattcttt cttatcaggt
     5641 aggatatctt tataggcata atcggtaaaa ggaggtacaa aacccttttt gacgcgggtt
     5701 gattcaccca aattctccat catttctttt aatgggctcg tattaaaccc ggttgaccaa
     5761 tgagtcgaat aaaaaacgta gcattgatat tctgcccttg tgataccaac gtagattaag
     5821 cgtcttatct cacgtcggtc attggctttc cacaggtcta atccttcttg atccaaatcc
     5881 aatggatgac gcaaagttct gatacaatca ttattcccag catgccctcc cggcttcacc
     5941 ttaaccaatt gatcatcctt caatgctgaa tcttttagcc cttcaataag aacaatcggg
     6001 aactccaggc ctttgctttt gtgcaaggtc atgatggaaa ccgcttcttc atcgctttca
     6061 atcctcaggg tatattcccc ttcttctgaa tcttgcgaat cggcacgtcg gagccagtcc
     6121 atcaattctt caggatcaag gccttttcga tactgcaaat catgcaatat ttctgaaagt
     6181 tgcaggatat tggcaagact cctctgtgaa gcataattta gggaaagatt ctcttcaaca
     6241 ctaaagtcct ccattaggat cataaaagcc tgatagaatc cttgataaag cattatttca
     6301 tgataatgag aaaatttttc aatagctttt atctcatcaa taaataaaac cggcaggttt
     6361 tcagcggtca gatttttata tacagtattt aaaaaggtaa gaaacagagc agaacgaata
     6421 ttcttgatgt ccggcttcaa catagcctgc aataccaatc ccatttcttt agcttccgta
     6481 ctcttcgtca ccaaagtatc gtcaacactc acagccggaa ttcccagagc atgcaatttc
     6541 tccttgattg cctttgctga atacttgtct cgggtaagga ctgcaatatc tcttggtcgg
     6601 acagccctta actgattatc ttctttatct gccaattgga tttgttcttc aggattaagc
     6661 aggtatttga ccgtctcaac taattgagtt tgacttgtag cagcatcatt agcattgcag
     6721 aaaaggaggg tttcctcaac ttcctcttgc cttctcaaaa acttcttatt atgcttattt
     6781 gcctgaatct ctgtataacc aatataaata cccgtctcct tttcagcctc ataaccaaac
     6841 acattatcac cagaggcttt gaagaattca ttggcagcct ctatcatcct tgttgaagac
     6901 cgaaagttgg tattcatcgt ataaatatga tccacttttg acttggcatg aaggtagctt
     6961 tcaacatcag catctctgaa tgaataaata ctttgttttg gatctccaat cagaaataag
     7021 gtagtatcat catcatactg atcatcgata aacaacttct caaatagttg gaattggatg
     7081 acatcggtat cctgaaattc atcgataaag gcaacacgat atttttgcct tatcagttct
     7141 ttcaattttt tattattccc ggcaacaatc gaacgatgaa gattaaggat tagatgatcg
     7201 aaggtgagga ccccggaatc gttgatagat tgatttaaaa catccagata ataatcccta
     7261 ttagcatata ggattgctac tatgacttta tttttttcat tgttca
//
LOCUS       DAOQMC010000119         7269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4220_length_7269_cov_5.045051, whole genome shotgun sequence.
ACCESSION   DAOQMC010000119 DAOQMC010000000
VERSION     DAOQMC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4220_length_7269_cov_5.045051"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKV31_05965"
     CDS             <1..684
                     /locus_tag="PKV31_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNB92434.1"
                     /translation="INTPPGYAVNVAKHIADLTGIPFITAPNKFESLAAHDAIVSAHG
                     ALKTVAVSMMKIANDIRLLASGPRCGIGEISIPDNEPGSSIMPGKVNPTQCEAMTMVA
                     AQVMGNDVAINVGGSTGHFELNVFKPVMIRNFIHSAKLIGDVCVSFDERCARGIEPIL
                     PNIKNNLDHSLMLVTALNTRIGYYKAAEIAQTAHREHKTLKQVAVELGYVSPEDFDAW
                     VRPEDMVGL"
     gene            complement(789..1832)
                     /locus_tag="PKV31_05970"
     CDS             complement(789..1832)
                     /locus_tag="PKV31_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92435.1"
                     /translation="MKKLFFLFFASIMITACTKDDPGGDPGIYDNGVFVVNEGPFGTG
                     TGTLTFIGDDGNLTQKVFEKENGNVVLGNIAQSMIWHNNRYYIVVNNANKIVVADKDM
                     KVVGEITGLTGPRFMVAQGNKAYISEWGENYEKGGVAIADLTSLKVVKQIPTGKGPEA
                     LLLDGNQLLVPNGGSYNASTFETLPDSTISVIDIGSESVTKVISVKYNPNSIIKAGNG
                     YLVASAEKSFGTNNGSIYFIRSSDNSVTKVAGATTGSYAKLHKVDETKSVVISGFAEA
                     LLLTFDAAAVSMTQKALDFAYSVDYDIKRKLIYIADAGDFSSEGKVITYNVNGTKEKE
                     FAAGIIPGNFCFR"
     regulatory      complement(1955..2167)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2391..2942
                     /locus_tag="PKV31_05975"
     CDS             2391..2942
                     /locus_tag="PKV31_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNB92436.1"
                     /translation="MKFFNIILLFAAVSLVSCSSPRKSISTGRSPAKSHHKDLSGAER
                     EKASARTPEGYLRAEGLAADKRLIRLVDDWLGVPHRIGGKDKSGIDCSGFVTVAMNKV
                     YSKDFSGPSAVMATRVKAIRKNELKEGDLVFFKINGPKVSHVGLYLSDGYFVHATVKK
                     GVMISSLSEPYYEKFYSGSGRVL"
     gene            complement(3396..4286)
                     /locus_tag="PKV31_05980"
     CDS             complement(3396..4286)
                     /locus_tag="PKV31_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92437.1"
                     /translation="MAIEAVIDLGTNSFNLLIGRSEDGKKEIMYRESRIVQLAKEGIT
                     PIGDASLQRAMDALDVFQEKISNYKAERVRVIGTSALRSASNSDDVISKIRSYYHWEV
                     SIIDGEAEARYIFVGTMEALQISDETCLIMDIGGGSVEFILTKAGKMLWWTSVPIGVQ
                     YLYLHFGRNVPTMSEEVAALERLLDLELAGLKKAIRQFKPERLIGASGAFDELLQMSA
                     IPAGDKFTRLDTPIFYENFEKLQKQVTERRNVSTMRTEERPEIVMTGFLLIRYVLQLI
                     KVPEIIATPFALREGVFFMG"
     gene            complement(4288..4899)
                     /locus_tag="PKV31_05985"
     CDS             complement(4288..4899)
                     /locus_tag="PKV31_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNB92438.1"
                     /translation="MRLLILCLLITGIIYTGIGQSNFTGGIIAGGSLSQVTGDEAFGY
                     NKASYLVGVKAGYHFKTRLNIEVEFLYNIKGSARGRRFNYLPYWTLEFKYLEIPLLIG
                     YKDWRNTKGFYHMVFYGGLSYAKMIDSKLTNPKYEPLMTGNLNKNEFSFILGATYNVN
                     KHLGFTFRYNRAITKVWENSIVDDRFSRWMKNYQISLAAQYMF"
     gene            5051..5290
                     /locus_tag="PKV31_05990"
     CDS             5051..5290
                     /locus_tag="PKV31_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92439.1"
                     /translation="MSNKEKANIKTLVGEIHSLGYRVIFEKGNFQSGHCIVLDRKVIV
                     INKFLSDKVKVLTLKDLLYQIRKEESEHGKFSVPN"
     gene            5293..6078
                     /locus_tag="PKV31_05995"
     CDS             5293..6078
                     /locus_tag="PKV31_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB92440.1"
                     /translation="MKRKEGIVTLTGTGTSQGVPVISCTCAVCRSDDPRDRRFRTSAH
                     VSFETGAFMVDIGPDFRLQCLGNNIDRLDGIFVTHEHIDHVNGLDDIRPFNYIQKKVM
                     PIYGEERVLNEIRKRNSYIFEPSTYAGLPMLELRNIEAFSPVDFQGMLVMPMRIMHDR
                     LPILGYQFGNFAYVTDAKTFVPETVSILREMDTVVINALHHRKHHSHLNLEEALDFIS
                     ENRLKKVYLTHISHQMGQYKEINQKLPGGVELAYDGLKINFRY"
     gene            complement(6106..6447)
                     /locus_tag="PKV31_06000"
     CDS             complement(6106..6447)
                     /locus_tag="PKV31_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB92441.1"
                     /translation="MTENSLLQSEKLYFSISEIAESFGVSASLIRYWETEFTFLKPHK
                     NSKGDRRFTKQNIEQIQLIYHLVKERGFTLEGAKNEINEIRKRQMTIKSLSNKLKSIK
                     QGLEGLKKNLE"
     gene            6969..7250
                     /locus_tag="PKV31_06005"
     CDS             6969..7250
                     /locus_tag="PKV31_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92442.1"
                     /translation="MSDFLKKMKSIFVEAVDDGSQPVNAETNSEVKAETNNTDTSKAY
                     SENLTNHTSSNTEGQQSDQFYKVLFGALEANNQPGFDYLEFKKALIFGS"
BASE COUNT         2042 a         1465 c         1474 g         2288 t
ORIGIN      
        1 atcaatacgc ctcccggtta tgcagtcaat gtagcaaagc atatcgccga tctgacagga
       61 atccctttca ttacggcacc caacaaattt gaatcgctcg cagctcacga tgccattgtg
      121 tcagcacatg gtgccctaaa aactgttgca gttagtatga tgaagatcgc caatgatatc
      181 cgtctgctgg cttcaggccc acgttgcggc attggagaaa tttctattcc tgacaatgag
      241 ccgggaagtt ccatcatgcc cggtaaggtc aatcctacac aatgtgaagc tatgacaatg
      301 gtggcagctc aggtaatggg taacgacgtg gccatcaatg tcggtggatc tacaggacac
      361 tttgagttga atgtattcaa accggtgatg atcagaaact ttattcacag tgcaaaattg
      421 ataggcgatg tatgtgtatc ctttgatgaa cgttgtgcca ggggaataga acctattctt
      481 cccaacataa agaacaacct ggatcattct cttatgctgg ttacagccct taatacaagg
      541 atcggctatt ataaagcagc cgagatagct cagacggctc atagagaaca caagacactt
      601 aagcaggtgg ctgtagaact tggctatgtt agccctgagg attttgatgc atgggtaagg
      661 ccggaagaca tggtagggct gtgatttgtc agctaaagta aaaaaaaggc ggcccttgcg
      721 accgcctttt ttttttatgc aatcaggata ctgattagcc tgtgaattat tcggctttga
      781 ccaggtgatt atctgaaaca aaagttaccc ggaataattc ctgctgcaaa ctctttttct
      841 tttgttccat ttacattata ggttatgacc tttccctcac ttgagaaatc acctgcatca
      901 gcgatgtata tcaattttcg ctttatatcg tagtccactg aatacgcaaa gtcaagggcc
      961 ttttgagtca tgcttacagc tgcagcatca aaagttagta acaaagcttc agcaaagccg
     1021 gagataacaa cagacttggt ttcatcgacc ttgtgcagtt tcgcatagct tcctgttgtt
     1081 gctcctgcaa ccttcgtaac tgaattgtca gaactacgga taaaatatat gctgccattg
     1141 ttggtaccga aactcttttc ggccgaagca accaaatatc cattgcccgc tttgatgata
     1201 ctgtttggat tgtatttgac agaaatgact ttggtcactg attcacttcc tatgtcgatg
     1261 acagatatgg tggagtcggg tagtgtttcg aaggtggagg cgttataaga accgccattt
     1321 ggtaccagga gttgattgcc atccaatagt aaggcttcgg ggccttttcc tgtaggtatt
     1381 tgtttcacaa ccttaagaga agtcaagtct gctatagcca cgccaccttt ttcataattt
     1441 tctccccatt cgctgatata tgccttattg ccctgagcta ccatgaaacg cggacctgtc
     1501 agtccggtga tttcaccgac cactttcatg tccttatctg ctactacaat cttattggca
     1561 ttgttgacca cgatataata tcggttgttg tgccagatca tcgactgagc tatgtttccg
     1621 agcaccacat ttccattttc cttttcgaag accttttggg tcagattgcc atcatctcca
     1681 atgaatgtga gcgtgcccgt tccggtccca aagggtccct cattgactac aaaaacacca
     1741 ttgtcgtata tacccggatc cccccccgga tcatccttag tacaggcagt aatcataatt
     1801 gaagcgaaga ataaaaaaaa tagttttttc atcagaataa aattttgggc ctttatagcc
     1861 ggttaataaa tcaattttat ctctgaagaa tagaagtaaa atacaaagcg agaagcataa
     1921 gccctgacca tgtaccaatc cttttctccg aaagatcgaa cacatcgcaa caggcaggtc
     1981 ttctggcttc atcatgcggt caggcgcctt cccatttatc cataaacagt ggcatgtaag
     2041 gcctttccat tattccgtaa acacggaaaa gatgttacag cagcgggaac tgcccgggat
     2101 tctcacccgg ttcccattta attccaaagc atattatact caggaaacca aattgcgcga
     2161 caaagataat atgaatatat gtatttggat taaccataaa ttaaaattgt caaataactt
     2221 cagtaatttt tcggctgaat gaatcatcgg cttctctttc ctctgttttg tacaaaagcc
     2281 tcacttaccc agctaaatat acatttttat ttatatatat gaatatttta acatttaata
     2341 tgttgattaa taacgtatga tagattaatt ttgtcgttaa tatataagtg atgaaattct
     2401 ttaatatcat attgttattt gcagctgttt ccttggtttc ctgtagttct ccaaggaaga
     2461 gcatttcaac cggcaggtct cccgctaaaa gtcatcacaa ggatttatcg ggtgcagagc
     2521 gggaaaaggc atcggccaga actccggaag gttatctcag agcagaaggc cttgctgctg
     2581 ataaaaggct cattcgtctg gtagatgatt ggttgggtgt gccacacaga ataggaggga
     2641 aggataagag tggaattgat tgttcgggct tcgtaacagt tgccatgaac aaagtatata
     2701 gtaaagactt ttccgggcct tcagcggtga tggctaccag ggtgaaggcg atcaggaaga
     2761 atgaattgaa agagggagat cttgtctttt ttaagattaa tggccctaaa gtttcccacg
     2821 tcggtctcta tctttctgat ggttatttcg tacatgcaac tgtcaaaaaa ggcgttatga
     2881 tttcttcatt gagtgagcct tattatgaaa aattttattc aggttcagga agggtgctgt
     2941 gattaaggcg gttttgttct taatcttttc agttttcgtt atttagacag tcagatgttg
     3001 ataggtaaat tttaatatgt taatttttga aaacaagata tttagatatt tattgttttt
     3061 atttacaatc ccctgatttg aatgttttat tctattaatt ctttctatca acacatcgac
     3121 gctttcatat atgtgacaaa aatattttaa gctgtactga tgattgtacc gttgttttaa
     3181 ctgtccggca attggccaaa gttgattcct tttagtgaat tcaggtttgc taatcgatgt
     3241 caattgtcaa cccaacgact gttcttccat ttgatttctt tcaagctgta aaataactta
     3301 ttgtaagcca ggtgtttgca ccttcatgcc gagaagtatt tcaattctca aatctcttac
     3361 ccaatgtatc cgaccccgga ctcttcaact tttttttatc ccataaaaaa caccccctct
     3421 ctcaatgcaa agggcgtagc gattatttcc gggaccttaa ttaattgcaa tacgtaacgt
     3481 atcaatagga atcctgtcat cacgatttca ggcctttctt ccgttctcat tgtggataca
     3541 ttcctccgtt ccgtgacttg tttttgcagt ttttcaaagt tttcatagaa tattggagtg
     3601 tccaatcggg taaatttatc tccggcagga atagccgaca tttggagtaa ttcatcaaat
     3661 gcaccggaag ctccgataag tcgctccggt ttgaattgtc tgatggcttt ctttagtcct
     3721 gcgagttcga gatccaataa cctttctagg gcggcaacct cttcagacat ggtagggaca
     3781 ttacgcccaa agtgcagata aagatattga acgcctatag gcacactcgt ccaccacagc
     3841 atcttacccg ctttagtaag aatgaattcc acacttccgc ccccgatgtc catgatcagg
     3901 caagtttcgt cagatatctg caaagcttcc atggtgccga caaatatata ccttgcttct
     3961 gcctcaccat cgatgattga aacttcccaa tgataatatg accggatctt acttattaca
     4021 tcatcactat ttgaagcgct ccgcaaagca gaagtgccga tgacccgcac tctctcagct
     4081 ttgtagttgc ttattttttc ttgaaataca tccaatgcat ccattgctct ttgaagggat
     4141 gcgtctccaa tgggcgttat gccttccttt gccagctgga cgatccgact ttcccggtac
     4201 atgatctctt ttttgccatc ctccgatctg ccgattagta aattgaagga atttgtacca
     4261 aggtctatga ctgcctctat agccatatca gaacatatac tgggcagcca gactgatttg
     4321 ataatttttc atccagcggg aaaatcgatc gtcaacaata ctgttttccc aaaccttagt
     4381 gattgccctg ttgtacctga atgtgaatcc caggtgctta ttgacgttgt aagtcgctcc
     4441 caggataaag gaaaattcgt ttttgttgag gttgcctgtc ataaggggct cgtattttgg
     4501 attggtcagt ttactgtcaa tcatcttggc ataggacaac ccgccataga aaaccatgtg
     4561 gtagaaacct ttagtgttgc gccagtcctt ataaccaatc aaaagaggaa tctcgagata
     4621 cttgaattca agggtccaat agggaagata gttgaatcgc cgtccacgtg cgctgccttt
     4681 gatgttgtag agaaactcca cttcaatatt gagtcgggtc ttgaagtggt aacctgcctt
     4741 cacgcctaca agataggagg ccttgttata tccaaatgct tcgtcacctg tcacttgtga
     4801 gagacttcct ccggcgatga ttcccccggt aaagttactc tggcctatac ccgtatatat
     4861 aattccggtg atcaacaggc ataaaatcaa aagacgcata tcctttcttt aatactgcaa
     4921 atgtaaataa ataacgtaca agtctacaat ttgtgattga tgtggtatgg aatatcgtca
     4981 ctcccctcta aagccaattt cattttttcc cgattaacat tatcttagcg gttaattact
     5041 cttcatatct atgtcaaata aggagaaggc caatatcaag acactcgtcg gtgagattca
     5101 ttctttgggt tatagagtga tttttgaaaa aggaaatttt caatccgggc attgcatcgt
     5161 tttggatagg aaagtcattg taatcaataa atttctatcc gataaggtta aggtcctcac
     5221 attgaaagat ctgttatatc aaatcaggaa agaagaaagt gagcacggca aattttccgt
     5281 ccccaattga cgatgaaaag gaaagaaggc attgttacat taaccggtac ggggacatct
     5341 cagggggttc ctgtcatttc ctgtacatgc gcggtatgca ggtcggatga ccctcgggat
     5401 cggcgtttca ggacctcggc tcatgttagc tttgaaaccg gcgccttcat ggttgacata
     5461 ggtccggatt ttcgtctgca atgtctggga aataatattg accgactgga tgggattttt
     5521 gtcacgcatg aacacatcga tcatgtcaac ggtttggatg atatccgacc cttcaattat
     5581 attcagaaga aggtcatgcc catctatggt gaagaacgtg tcctgaatga gatcagaaag
     5641 cgcaacagtt acatcttcga accttcaacc tatgcaggtc tgccaatgct tgaattgaga
     5701 aatattgaag catttagtcc ggttgatttt cagggtatgt tggttatgcc catgcgtatc
     5761 atgcacgacc gtctgccaat cctgggatat caatttggca attttgccta tgtgactgat
     5821 gccaaaacct ttgttcctga aacagtctcc attttgagag agatggatac agtagtgatc
     5881 aacgcattgc atcatcgtaa gcatcattca catctgaatc ttgaggaagc cttggatttt
     5941 atttccgaaa accggctcaa aaaagtttac ctcactcata ttagccatca aatgggtcag
     6001 tacaaggaaa taaatcaaaa acttccgggc ggggtcgaat tagcctatga tggattgaaa
     6061 atcaatttca ggtattaatc aatagggaaa gtataaattg gatgattatt caagattctt
     6121 tttcaaacct tccagccctt gtttgatgga ttttaatttg ttggaaagtg acttgatggt
     6181 catttgcctt tttcggattt cgtttatttc gtttttggct ccctccagtg taaagcctct
     6241 ttccttcaca agatggtaga taagctggat ttgctcgata ttctgtttgg taaatcgcct
     6301 gtcacccttg ctgtttttat ggggtttgag gaaggtgaat tctgtctccc aataacgaat
     6361 gagagaagca gatacgccaa atgactcagc tatctcactg attgaaaaat ataatttttc
     6421 gctttgcaat agactgtttt cggtcatagt tgtaagtcaa cttttaataa taatggtggg
     6481 atggcttatt atcaataagt tacattacaa agatacaatt attggtttaa tatattgaat
     6541 ttttatgtat aatttttttt taaatgttta tgatgattgt tgttgcagtc gtttcgacga
     6601 aagttatcgt tcaggtcaga aattactctt ggaacatttg aatgaaaagc cgggttgaat
     6661 atttctttat aattagatct caaagtaaaa aagggagacc attggtcgga ctatttactg
     6721 aattttgtcg atttttttga ccgccaggaa actatcggca tttaattaaa attaacatat
     6781 ttcgataatt tcatggaatg tttcgaaaaa tatcgtgttt ttgatactta ttagggtaaa
     6841 aagtagtctt ttctttgaat cgtcacatcg aattttttgt actatggctt tttttcttac
     6901 cttagcaatc cgtataggac gatataaata cagttccatt gatttttctt gcatagtttt
     6961 gagtcaaaat gagtgatttt ttaaagaaaa tgaagtctat ttttgttgaa gcagttgacg
     7021 atggcagcca accggtaaat gctgagacaa attcagaagt gaaagctgaa accaacaaca
     7081 ctgacacttc aaaagcgtat tcggaaaact tgacaaatca tacaagcagc aacaccgaag
     7141 ggcaacaatc tgatcaattt tacaaagttc tttttggtgc tttggaagct aataaccaac
     7201 cgggatttga ttatcttgag ttcaaaaagg ctttgatctt tggatcttga tttggattgt
     7261 agatagccg
//
LOCUS       DAOQMC010000120         7014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4473_length_7014_cov_5.081765, whole genome shotgun sequence.
ACCESSION   DAOQMC010000120 DAOQMC010000000
VERSION     DAOQMC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4473_length_7014_cov_5.081765"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PKV31_06010"
     CDS             <1..1172
                     /locus_tag="PKV31_06010"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNB92443.1"
                     /translation="ITAQADYSHPFSKKLSLDLGIKSISRRIESYYVYDTFNLATNTQ
                     IKDEMRSNSFGYLQNVLAGYAEATWAITPKFGLKAGVRYEGTTVEGDIFKEVQSLNNN
                     YYNILPSGTFSYKWGSNGLKLSYNQRLQRPSLFYLNPYSNLADPKNITVGNPKLNAEL
                     SHNVEVGYNKFFGLSSVNIAVYRRFTNNAIEAVRKISGDTIVTTYYNQANNKNTGLNV
                     SGNLMKGYKFMIGGNFDISYVEVDNNALGINNSGINYGINGFLNWTIYEAWGIQAYGG
                     FRGPTITSQGKSTSFYFYGIGAKRDLLNKKASLSIGVDNPFTPYQVNKTELNVYGTQF
                     KSENRFYAFGGRISFNWMFGKMSFSNKSNEKGIQNDDLKSGENGQGPGGQMGRMK"
     gene            1454..2101
                     /gene="deoC"
                     /locus_tag="PKV31_06015"
     CDS             1454..2101
                     /gene="deoC"
                     /locus_tag="PKV31_06015"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNB92444.1"
                     /translation="MQLNKYIDLTYLKPDSTRQRIKELCTEAIENDVFSVCIPPYYIP
                     MAAELLEDHSVKVCTVIGFPYGYDATPTKAEAVKKAVVEKVDEIDVVVNIAAIKNGDW
                     SYVANDVDSITRLGHMRNLLVKLIIEVNLLTEDEIKRICDIAVQKEVDFIKTSTGTVG
                     DAVTVEDIILLRSLIPATTKVKASGGIRTREQAMALIDAGADRLGTSNVSSILLP"
     gene            2098..2490
                     /locus_tag="PKV31_06020"
     CDS             2098..2490
                     /locus_tag="PKV31_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92445.1"
                     /translation="MMRKACLCFLFFIFLCHLGYAQQVAITMDSNTETAVKEYNNNQL
                     AQTMIPVYTIQFKITSDRHEMERELATFRNEYNLKPKWTQKGPYYYLKAGAYRTKLEG
                     YADMKAISTKYPGALFIVEKVKKQLILD"
     gene            2522..3928
                     /gene="rodA"
                     /locus_tag="PKV31_06025"
     CDS             2522..3928
                     /gene="rodA"
                     /locus_tag="PKV31_06025"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNB92446.1"
                     /translation="MNKRAVQNETGRVDWVLLSLYLALVAIGWTMIYAVSHDKNTSGS
                     ILSLDYNGGRQIIWIGISLFVLFFILVFDSKIWRTFSYGFYAIGIILLTLVLFFGKVI
                     NGQKAWFGIGMFTFQPAEIAKFGTALALANLLSDYKMKLSEPRAAMVAISLVLFPPLL
                     IMLQPDAGSALVFFSFFILLFREGLNASLYIIILSFAGLFILSLLFGFDNTYLVILIA
                     GLAIMVYHLPNRLYYMLAVGMLGIGGFIAVNSGNAPELLGVLTGILLVLAGILVWKKK
                     SQLVFVLVSVLTIASLTSFFSNFVFNILEPHQQERINVWLHPERCDPRGSLYNVLQSK
                     MAIGSGGLSGKGFLNGTLTKLNYVPEQTTDFIFCTVGEEHGFIGTLIVISLYLTLLLR
                     LSFVAERQRLNFSRNYIWGVAGILFFHFLINIGMTMGLVPIIGIPLPFVSYGGSSLIG
                     FTVLLGVVIKLDSIRNKD"
     gene            4068..5207
                     /gene="tgt"
                     /locus_tag="PKV31_06030"
     CDS             4068..5207
                     /gene="tgt"
                     /locus_tag="PKV31_06030"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNB92447.1"
                     /translation="MTNKFELIHTDDKSRARTGILSTAHGDILTPVFMPVGTVGTVKG
                     VHQSELSDKIKARIILGNTYHLYLRPGVETVAGAGGLHKFIGWDKPVLTDSGGYQVFS
                     LAHRRKIREEGVTFQSHIDGSRHLFTPESATEVQLQLGADIIMAFDECTPYPCEYKYA
                     ERSMHLTHSWLDRCLSYFDAIDEFPHGYRPTLAPIVQGSTYSDLRRQSAEYIASKSRH
                     VNAIGGLSVGEPAEEMYRITEEVTTILPKEAARYLMGVGTPANILTCIGLGIDLFDCV
                     LPSRNARHGLLYTWDGIINIKNEKWKMDYSPIDSTSRADTSRQHSRAYLRHLIHVGEL
                     LGAQIATIHNLAFFLELVDTARERILDGTFADWSMQVIPRLEHRL"
     gene            5324..6139
                     /locus_tag="PKV31_06035"
     CDS             5324..6139
                     /locus_tag="PKV31_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92448.1"
                     /translation="MEVKKVVITGGSRGIGEAIALKLAGQGMHIAILAKTTEPQPKLK
                     GTIYSVAEKIELQGGKAYPHQLDIRDDAAISQAISTVAGQMGGIDILINNASAISLTN
                     TEDTSAKRFDLMFDINVRGTFLVTQACIPHLRKSSNPHILTLSPPLSVEPRWFENHVA
                     YTISKYNMSLLTLGWAAELKEDGIAANSLWPATLIGTAAIENMAGGDQLVRMTRKPQI
                     MADAASLILEKNARDFTGNFVIDEDILKQHGITDFSSYDSVPGMQPLPDLFIG"
     gene            complement(6150..>7014)
                     /locus_tag="PKV31_06040"
     CDS             complement(6150..>7014)
                     /locus_tag="PKV31_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92449.1"
                     /translation="KHNGDCRIVKEFKSPNCDAAEQCELGQPTILPSSCNDSGQFYVQ
                     IDFSYADVSDSFKIKGNGHNYGHFSYSDLPVVLGPFDGNVDVVYEFGIIDLSDNKCYT
                     SGYISPPYCQNGFPYTISQIGISRLICIDSVSYTATIELLTDGLPDVSFKLFQENSFI
                     GNYYVGSFPVDVTLKKSHLPRVKIADEKEYRNFAISVYQHPDCLLNVNENEGANARCY
                     PNPLSSHINLEWQGQAFSFRIFDLSGREHLKGQCVDKTEVDMSRLDSGVYIVVLNGGR
                     TNSMFKMIKLY"
BASE COUNT         2063 a         1550 c         1515 g         1886 t
ORIGIN      
        1 aaatcacagc tcaggcggat tactcacatc cattctccaa gaagttgagt cttgatcttg
       61 gtattaaatc aatatcgaga aggattgaaa gctattatgt atatgacacc ttcaatctgg
      121 ctactaatac ccaaatcaag gatgaaatgc gctccaattc ctttggttat ttgcaaaacg
      181 tgttggcggg ttatgctgaa gcaacctggg ccatcacgcc caaattcggg ttaaaagcag
      241 gtgttagata tgaagggacc actgtggaag gagacatctt caaggaggtt caaagcctga
      301 ataataacta ctacaatata ctgccaagcg gaactttctc ctacaaatgg ggaagtaacg
      361 gtctcaagct ttcctataat caaagactgc aaaggccatc tttattctac ctgaatcctt
      421 attccaatct ggccgatccg aagaatataa cagttggaaa tccaaaattg aatgccgaat
      481 tgtctcataa tgtggaagta ggatacaaca aatttttcgg actcagctca gtcaatattg
      541 ccgtatatcg ccgttttacc aacaatgcta ttgaggccgt caggaaaatc agtggagaca
      601 ccatcgtcac cacctattat aaccaggcca acaataaaaa cacaggactc aatgtatccg
      661 gtaacctcat gaaaggctac aagtttatga ttggtggcaa ttttgacatc tcttatgtcg
      721 aagtagataa taacgccctc ggcatcaaca acagcggtat caattacggt atcaatggat
      781 ttctcaactg gaccatctat gaagcgtggg gcatccaggc ttacggtggc ttccgtggtc
      841 cgaccatcac ctcacaaggc aaatcaacga gcttttattt ttatggcatc ggtgcaaaac
      901 gcgacctgct caacaagaaa gcttctctgt ctataggtgt tgacaatcct tttacccctt
      961 accaggtcaa taaaaccgaa ttaaatgtgt atggcacaca attcaagtca gaaaacagat
     1021 tctatgcatt cggcggccgt atcagcttca actggatgtt tggaaagatg agtttttcaa
     1081 ataagtccaa tgaaaaaggt attcagaatg acgaccttaa atcaggcgaa aatggtcaag
     1141 gtccgggagg gcagatgggg cgaatgaaat aagacagaac ggcacggcag gttatgttac
     1201 catcaaggta agaccttcat cctgaggcga attgtgattt ggcagtttta gaacaaacca
     1261 caattcgtct ttttacattt tttacggcat cgactaaaaa aattttcttc aaggaacatt
     1321 ccattgaaat ataattgtaa aatttacaca ttttcaattg attatcataa atcaatacta
     1381 attctggctg tgatttgatt ttccgctgtg gctaatttta ttatctttgt gattccgaaa
     1441 atcgaaatcc aatatgcaat taaataaata catagacctg acctatctta aaccggattc
     1501 aaccaggcag cgtattaagg agttatgtac cgaggctata gaaaatgatg tattctctgt
     1561 ctgtattccg ccttactaca tacctatggc tgctgagttg ctggaagatc acagtgttaa
     1621 agtgtgtaca gttatcggat tcccatatgg ttatgatgcc acaccgacca aggctgaggc
     1681 agtcaagaaa gcagtcgtgg aaaaagtaga tgaaatcgat gtcgttgtca atattgcagc
     1741 catcaaaaat ggagattggt cctatgtcgc caatgatgtg gacagcatta cccgcttggg
     1801 acatatgcgc aacctgctgg tgaagctaat cattgaggtc aacctgctta cggaagacga
     1861 gatcaaaaga atatgtgata ttgcagtaca gaaggaagtg gattttatca agacaagtac
     1921 cggtacggtc ggtgatgcag tcactgtaga agacataatc ctgctccgca gtttgattcc
     1981 ggccacaact aaggtcaagg ccagcggtgg cattcggacc agagaacagg ccatggccct
     2041 gattgatgcc ggagcagaca gattaggcac atctaatgtt tcatctattt tattaccttg
     2101 atgcgaaaag cctgtctctg cttcctgttt tttattttct tatgtcacct gggttatgcc
     2161 cagcaggttg caatcaccat ggattcaaat acagaaactg cagtcaaaga gtacaacaac
     2221 aaccagcttg ctcagaccat gatcccggtg tacacaattc aattcaagat tacttccgac
     2281 agacatgaaa tggaacgcga gttggcaaca ttcaggaatg aatataatct taagccaaaa
     2341 tggactcaaa aaggccccta ttattatctg aaagcaggag cctaccgcac caaactcgaa
     2401 ggatatgccg acatgaaggc tatatcaaca aaataccccg gagcactttt tatcgtagaa
     2461 aaagtcaaaa aacaactcat cctcgattaa ttgactgcat cgaataacaa aaccaccggc
     2521 aatgaataaa agggctgttc agaatgaaac cggcagagtg gactgggttt tgctctccct
     2581 ttatcttgcc cttgttgcta ttgggtggac catgatatat gccgtcagtc atgacaagaa
     2641 cacttcagga agtatactga gccttgatta taatggaggc aggcaaatta tctggatagg
     2701 aatatccttg tttgtcctgt tttttattct ggttttcgac agcaaaattt ggcgtacctt
     2761 ctcttacggt ttctatgcca tcggcatcat actattgaca ttagttctat tcttcggaaa
     2821 ggtcatcaac gggcaaaaag cgtggttcgg tataggcatg tttacctttc agccagcaga
     2881 gattgccaag tttggaactg ccttagccct ggccaatctg ctcagtgatt ataaaatgaa
     2941 gctttctgag ccaagggctg ccatggtggc gatttcctta gttttattcc cgccgttgct
     3001 cattatgctc cagcccgatg caggctctgc cctagtattc ttttccttct tcatcctctt
     3061 attcagggag gggctcaatg cttccctgta tataattatc ctctcctttg cagggttgtt
     3121 tatcttatcc ttgttgttcg gatttgacaa tacctattta gttattttga ttgcaggtct
     3181 ggccatcatg gtataccacc tgcccaacag gctctattat atgttggcgg taggcatgtt
     3241 gggaatcgga ggcttcattg cggtcaacag tggtaatgca cctgagcttt tgggtgtact
     3301 gaccggcatc cttttggtcc tggcaggcat cctcgtatgg aaaaaaaagt cacagttggt
     3361 ctttgtattg gtgtctgtac ttaccattgc atccctgaca tctttctttt ctaattttgt
     3421 tttcaatatc ctggagcccc atcaacagga acggatcaat gtatggctcc atccggaaag
     3481 atgcgaccct cgcggctccc tatacaacgt gctccaatca aagatggcaa tcggctcagg
     3541 ggggctttcc ggaaaaggtt tcctgaatgg tacactgacc aagctaaact atgtcccgga
     3601 gcagacgacg gactttatat tttgtacggt cggagaagaa catggattca tagggacgct
     3661 gatcgtcata agtctttacc tgaccctgtt gttaaggctg tcgttcgtgg ctgaacgaca
     3721 aaggctcaat ttttcgagaa attatatctg gggagtggcc ggcatcctgt ttttccactt
     3781 cctgatcaat ataggtatga caatgggtct ggtcccaatc ataggtatac cgttgccatt
     3841 tgtcagttat ggcgggtcat ccttaattgg attcacagtc ctgctgggag tggtcatcaa
     3901 gcttgacagt atcaggaata aggattaata aaaacaggta agtcaggccg gataaataca
     3961 tcaactcata cgtataattg attagtttaa aattttttaa aacctgaaca agacctgcta
     4021 gataatttgt aaaataaaat gcggttattt atctctatta ttaaataatg acaaataagt
     4081 ttgaattaat ccatacagat gataaaagcc gggcacggac cgggatatta tctacggcac
     4141 atggtgacat actaacaccg gtattcatgc cggttggaac agtcggtacg gtcaagggtg
     4201 tccatcagtc ggaattgtcc gataaaataa aggcacggat catcctgggc aacacctatc
     4261 acctgtatct tcgaccgggt gtcgaaacag tagcaggtgc aggaggactc cacaaattta
     4321 tcgggtggga caagcctgtc ctgacggaca gtggaggtta tcaggttttt tccctggccc
     4381 acagaagaaa aatcagggag gaaggagtga cctttcagtc tcacatagat ggaagcagac
     4441 acctttttac ccctgaatcc gcaaccgaag tgcaattaca gctgggtgca gatatcataa
     4501 tggctttcga tgagtgtaca ccctaccctt gcgaatataa atatgccgaa agatccatgc
     4561 atctgacaca ttcctggctg gacaggtgcc tgtcgtattt tgatgccatc gatgaatttc
     4621 cacatgggta ccggcccact ctggctccaa tcgttcaggg aagtacctac agtgacttaa
     4681 gacgtcaatc ggctgaatat attgcttcca aatccagaca tgtcaatgca ataggcggcc
     4741 tctccgtagg ggaaccggca gaagaaatgt accgtattac cgaagaagtt acaactatat
     4801 tgccgaaaga ggcggcgaga tacctgatgg gtgtaggcac tccggcaaat atactcacct
     4861 gtatcggcct cggaatagac cttttcgact gcgtcctccc atcgcgcaat gctcgccatg
     4921 ggttactcta tacctgggac ggcatcatta atatcaagaa tgaaaaatgg aaaatggatt
     4981 attcgccgat cgacagtact tctcgggccg atacctccag acagcattca cgggcttatc
     5041 tcagacatct gattcatgta ggtgaattgc ttggcgcaca gattgcaacc atccacaact
     5101 tagcattctt tctggaattg gtggatacgg cccgggaaag aatattggac ggcacatttg
     5161 cagactggag tatgcaggta ataccacgac ttgagcatag attgtaagaa aggtaagcta
     5221 tgcaagtacg gatattttgc tatcttattt gagattttca caccgagaag aaaaatatcc
     5281 cgttcaaacc agaataaaat ggcctttttg cataacttta ggcatggaag taaaaaaagt
     5341 agttattacc ggtgggtccc ggggaatcgg tgaagccata gccttgaagc ttgcaggaca
     5401 gggaatgcat attgccatac tcgctaagac aacagagcca cagcccaaac taaaaggaac
     5461 tatctacagc gtcgctgaaa agattgaatt gcagggcggc aaggcctatc cccaccaatt
     5521 ggatatcagg gatgatgctg ccataagtca agcaatttct actgtagccg gacaaatggg
     5581 tggaatcgac atcctgatca acaacgcctc ggctatttcc ctcacaaata cggaggatac
     5641 atctgccaaa cgcttcgacc ttatgttcga tatcaatgtc agaggaacct tcctggtaac
     5701 acaggcctgt atcccgcacc tcagaaagag cagtaatcct catattctga ctctgtctcc
     5761 acccctcagt gtcgaaccac ggtggtttga aaaccatgtc gcttatacca tctccaaata
     5821 taacatgagt ctgttgacat taggatgggc agcagaactg aaggaagacg gcattgctgc
     5881 aaattcactt tggccggcaa cactcatagg taccgccgcc attgaaaaca tggcaggagg
     5941 agaccaactg gtaaggatga cccgcaaacc acagattatg gcagatgcag catctcttat
     6001 tctggaaaag aacgcaagag acttcaccgg caattttgtc attgacgaag atatattaaa
     6061 gcaacacggc attacagact tttcatccta tgattccgtg ccggggatgc agcccctacc
     6121 cgacctgttc atcggataaa ccttatttct caataaagtt tgatcatctt gaacatggaa
     6181 ttggtgcgac caccattaag gacgactata tatacgccgc tatcaagcct gctcatatcc
     6241 acttctgtct tgtctacaca ttgacctttg aggtgttccc ttccggacaa gtcaaatatc
     6301 ctgaacgaaa aggcttgtcc ctgccattca agattgatat gcgaggatag cgggttagga
     6361 taacatcttg cattggcccc ttcattttca tttacattca aaagacaatc aggatgctga
     6421 taaacggaga tggcaaaatt ccggtactct ttttcatccg caatcttgac tcgcggcaga
     6481 tgagatttct tcaaggtaac atcaaccgga aatgaaccga cataataatt tccaataaat
     6541 gaattctcct gaaaaagctt aaatgacaca tccggtaaac catcagtcag gagctcaatt
     6601 gtggcggtat aggaaaccga atcgatacat atcaaccgac ttataccgat ctgactaatg
     6661 gtataaggaa acccattctg gcaatatggc ggtgaaatat atcctgatgt atagcattta
     6721 ttatctgaca agtcgatgat tccaaattca tataccacat cgacattgcc atcaaatgga
     6781 cccagtacaa ccggcaggtc actatagcta aaatgaccgt aattatgccc attgcccttg
     6841 atcttgaagg agtctgatac atcagcatat gaaaaatcaa tctgaacata gaactgccct
     6901 gaatcattgc aactggaagg gagaatggtc ggctgaccca attcacattg ttctgctgca
     6961 tcgcaattgg gacttttgaa ttccttgacg atgcgacaat ccccattatg ctta
//
LOCUS       DAOQMC010000121         6987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4501_length_6987_cov_4.712204, whole genome shotgun sequence.
ACCESSION   DAOQMC010000121 DAOQMC010000000
VERSION     DAOQMC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_6987_cov_4.712204"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKV31_06045"
     CDS             complement(<1..454)
                     /locus_tag="PKV31_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB92450.1"
                     /translation="MHIKTMAEFAAEGRDPEVLFWVGCLGSYDARAQRVSAAFAQILD
                     NIGIEFAILGKEESCTGDPARRAGNEFIFQMLANQNIQTLTNYGVKKVVTTCPHCFNI
                     FKNEYPELGATFEVFHHTQFLSQLLQDKRLKIEGGSFDGQRITYHDSCY"
     gene            complement(460..1797)
                     /locus_tag="PKV31_06050"
     CDS             complement(460..1797)
                     /locus_tag="PKV31_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB92451.1"
                     /translation="MIISQILFLILLVWVFKEAVKRYGKVIRNIRLGRNDEPLNNKGG
                     FRRMLLVAFGQQKMFKRPLPAILHLFIYVAFIITQFDLLEQIIDGITGSHRILSHYLG
                     GLYSFMISFIELLSVFAFIATIAFLSRRNLLRIPRFTSKDLAGWARIDANLILIGEIL
                     LLTGIFCLNGADKALETKFPNEYPSTGFFLITGWLGPLLFGGMPEFFLHVISGFGWWL
                     HVLVVFGFILYLPGSKHLHILLAFPNTYLSSDNPRGRMINIESIENEVKNMMEIPLNH
                     APKETLADFGVHDIFQLTQKNLLDAYSCTECGRCTAVCPANLTGKKLSPRKIMMNVRD
                     RMEEVGCKLDDPNFSSMNGEIDNKIGFNDGRDLFNYIEKEEIRACTTCNACVEACPVL
                     INPLEIILQLRRYEILTQSSGPPDWVPMYTALENNGSPWQMTIERDDWIKELN"
     gene            1844..2347
                     /locus_tag="PKV31_06055"
     CDS             1844..2347
                     /locus_tag="PKV31_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB92452.1"
                     /translation="MQILNTFQMNQKIRRISFEILERNLGEEEIYFIGINNSGYALAQ
                     LLSDMLNGLEYNEIKSSVTRIRVKADQPTASDIYLEGIDVNELDNKCLILVDDVANTG
                     RTLFYAFQPLLRIIPRKLEVAVMVDRKHKLFPVHVDYVGLSLATTLKENIRVDLSDIK
                     NMTTSLN"
     gene            complement(2365..3555)
                     /locus_tag="PKV31_06060"
     CDS             complement(2365..3555)
                     /locus_tag="PKV31_06060"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HNB92453.1"
                     /translation="MEENKTSTSDKILWWLASADAQLLEGNRSEQRKFSLIGLSIFFT
                     WLFATAAWTYFFSTVTDSILIMLLGGFLMGGIVLTIDRVLIAGLQGSGEGSKTGAIAF
                     RVALALCLGAFMAQPALLYLFKKDINIQLTLDREVKKQAAVAEINRSLQPQLKPLLTQ
                     RDSLAGIQASLDSQVVALRNSYLAETDGSGGTGKVGIAAIARAKKAEYDAATLASNQW
                     KSSVAPLLSKINSNIDTMTSIRNKQIAAFDANDNDGFLARIEALQHLIESNLALRWRY
                     LLLLSIIMLIELAPVLSKLLLNTKVYNQRLILEEGYLLNLDREVFEKKYSEARKYSDA
                     MHQSNLDAINKIFEKSNSMMNQSSNDEMNKYELHHGKPATVNSLLTHMKQNLMGRHTS
                     SFEQ"
     gene            complement(3789..5927)
                     /locus_tag="PKV31_06065"
     CDS             complement(3789..5927)
                     /locus_tag="PKV31_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNB92454.1"
                     /translation="MNKTININLGGFPFTMDDDAYMLMERYLNAIKNHFAVSDSYQEI
                     MSDIESRLGELLHDKVSSKAIVTTADVEHIIKIMGRPEEFGAEAIDLPGEGSDQGTSG
                     DPIGTQRTRRLFRNLADKKLGGVCSGLAAYLGIKNANWIRLLFIILASSGGFGIPLYV
                     IMWIFVPPAKTSADYLAMKGEDINIHNIAEFLETEIKHFSNQVTDFAQDISQQFSNKG
                     KKSFRADRPSIDLEETFRSGTARLVSILRPFGLVLGGIFTLIFLGFWITLIVSYILAF
                     PLLGFINSTSPIINFLTSLNSIVIFAVPVVFVIFWALKIFFKTPVHRSIRQSLAIFWI
                     SNLICFILLSALTFREFKSYGAIEVKQDLGSLKDDQINISAITPDHRDGRLVLGDMEM
                     GNDNQLEINDNVRFNIYPTNGPAWSATKRVTSRGKDGVDAKKNADCVSYAISPKDAHT
                     LDVPTFFSIRPDCKYRFQQVEIDLFIPIGKYVTFPESLEWRLSEVKYDEAQMPNGWKF
                     IPGKRYKMSEMGLTCDDCTDEELTANMHADDYEYISQEDIPANAVVQSTEKEMDIALT
                     KDQLTTVSFDKIMNPTGHSVKFSKINLNIDQSMDDKVRIIRKLKFYSHTPIAVDQLDK
                     IYGIRQNGNDLIFSNTGLIFNDKKAFNPEITIKLLIPVGTKISFDENVNEFVEHVKFD
                     KEKITDNWHFLKNEVLEMGPKGLYCTNCND"
     gene            complement(5924..6295)
                     /locus_tag="PKV31_06070"
     CDS             complement(5924..6295)
                     /locus_tag="PKV31_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNB92455.1"
                     /translation="MNEKEMPDRETLKAENTKAQMRKGVLEMCILGIISGEELYTSDI
                     INKLKENELVVVEGTVYPLLTRLKNGGLLEYTWRESTTGPPRKYYRVTQAGEELLIEL
                     LESWNQLVKVVSQTTNKQSRS"
BASE COUNT         2013 a         1384 c         1460 g         2130 t
ORIGIN      
        1 ggtaacagga atcgtgatat gtaatacgct gtccatcaaa ggatccacct tcaattttca
       61 atcgtttgtc ctgcaacaac tggcttagga actgtgtatg gtggaacact tcaaacgttg
      121 ccccaagttc cggatattca tttttaaata tattaaaaca atgaggacag gtggtaacaa
      181 ctttttttac tccataattt gtcagagtct gaatattttg attggcaagc atctggaaga
      241 taaattcatt tccggcgcgt cgggcaggat ctcctgtgca agattcttct ttaccaagga
      301 tagcaaattc aatgccaata ttatccagta tctgagcaaa agcagctgag actcgctgtg
      361 ctctggcgtc ataactgccc agacatccta cccaaaacaa cacttcagga tctctacctt
      421 ccgctgcaaa ttctgccatt gtcttgatat gcatatttat taatttagtt cttttatcca
      481 atcatcccgc tcaatggtca tctgccacgg gcttccatta ttttcaagtg ctgtgtacat
      541 tggtacccaa tccggtggac cggagctttg ggtcaaaatt tcgtaacgtc ttagctgaag
      601 gatgatttca agtggattga tgaggacagg acaggcctca acgcaggcgt tgcaggttgt
      661 acaggccctt atttcttcct tttcgatgta attaaataag tctcggccat cattgaatcc
      721 aattttatta tcaatttcac cattcatgga actgaaattt ggatcatcaa gcttgcaacc
      781 tacttcttcc atgcggtcgc ggacattcat cattattttg cggggcgata atttttttcc
      841 ggtgaggttg gccgggcaca cagcagtgca acgtccgcat tcagtacatg aataagcgtc
      901 cagaaggttt ttttgcgtta actggaaaat atcgtgtaca ccaaaatcgg ccaatgtctc
      961 tttcggcgcg tgattcaacg ggatttccat catatttttt acttcgttct cgatggactc
     1021 tatgttgatc attcttcctc gtggattgtc ggatgaaaga taagtatttg ggaatgccag
     1081 taaaatatgc aggtgctttg atccgggaag atatagtatg aatccgaaaa cgaccagtac
     1141 atgtagccac caaccgaaac cactgattac atggaggaag aattcaggca tgcctccaaa
     1201 gagcaagggg cctaaccagc cggtaatcag aaagaatcct gtggaaggat attcatttgg
     1261 aaatttggtt tccagggctt tatcagcacc attgaggcag aaaataccgg tgaggagaag
     1321 gatctcccct ataaggatga ggttggcgtc aatcctggcc caccctgcaa gatccttact
     1381 ggtaaagcga gggatccgga gcaaattgcg ccttgacaaa aaggctattg tagcgataaa
     1441 agcaaatacg gacaacaatt cgatgaaact aatcatgaat gaatataatc cacccagata
     1501 gtgggataat attctgtggc ttccagtaat cccgtctatt atctgttcga gcaaatcaaa
     1561 ttgtgtgata ataaatgcca catatataaa taggtgcaaa atcgccggaa gtggacgttt
     1621 gaacatcttt tgctgaccga aagccaccag caacatacgg cggaacccac ccttgttatt
     1681 caacggttcg tcgttcctac cgagccgaat attccttatt accttgccat agcgctttac
     1741 ggcttccttg aacacccaga caagaagaat taaaaaaagt atctgagaaa tgatcatcgc
     1801 ctttaaatct tttataattt tatcctttac aaaagtaaat ataatgcaaa tattgaatac
     1861 cttccaaatg aatcaaaaga tcagaaggat ttcttttgag atcctggagc ggaatcttgg
     1921 tgaggaggaa atatatttta tagggatcaa caatagcgga tatgccctgg cgcaactgct
     1981 gtcagatatg cttaatggtt tggaatataa tgaaattaaa tcctccgtca caagaattcg
     2041 tgtgaaggct gatcagccga cagcttctga catatatctg gaggggattg atgtcaatga
     2101 gctggataat aaatgtctaa tattggtaga tgatgtggct aatacaggca ggacgttgtt
     2161 ttatgccttt caaccattat tgaggattat tccccgcaag cttgaggtag cggtcatggt
     2221 ggacagaaaa cataaactgt ttcctgtcca cgttgactat gtaggcctgt cactggcgac
     2281 taccctgaaa gaaaatatca gagtggacct gtcagatatc aagaatatga ctacctctct
     2341 caattgacgg cgctcaaatt tttttcattg ttcgaacgaa gaggtgtgcc ggcccatcag
     2401 gttttgtttc atatgagtta gcaagctatt gaccgtagcg ggtttgccgt gatgcaattc
     2461 gtatttattc atctcatcat ttgatgactg attcatcatt gagttggact tttcgaaaat
     2521 cttatttatg gcatccagat tggactgatg catggcatcg gaatattttc ttgcttcgct
     2581 gtattttttc tcaaacactt ccctgtcaag gttgagtagg tacccttctt cgagaattaa
     2641 tcgctggtta tatacctttg tgtttaacaa taacttgctt agcaccggtg ctaactcgat
     2701 caacatgatg atggagagca ataataaata acgccatctg agggcaaggt ttgattcgat
     2761 taaatgttgg agcgcctcaa tacgtgccag aaagccatca ttgtcgttag catcaaaggc
     2821 tgcaatttgt ttattccgga ttgatgtcat ggtatcaata ttggaattga tcttgctaag
     2881 caggggagct actgatgact tccattgatt ggaagccaat gtagcagcat catattccgc
     2941 tttttttgcg cgtgctattg cagcaatgcc taccttgcct gttccaccac taccatccgt
     3001 ttcagcaaga taagaatttc tgagggctac gacctgactg tctaaagatg cctgtatgcc
     3061 tgccagagaa tctctttggg tgaggagtgg ttttagttga ggttgtaaag accggttgat
     3121 ctctgctaca gcggcttgtt tttttacctc cctgtcaagt gttagctgga tattaatatc
     3181 ctttttgaat aaatataaca aagccggctg agccataaag gcaccaaggc aaagggccag
     3241 agctacacgg aaagcgatgg cgcccgtctt acttccttcc cctgaacctt gaagccctgc
     3301 aatcaggacc ctgtcgatgg tcagcacaat acctcccatc aggaagcccc ctagaagcat
     3361 aatcagaata ctgtctgtta cagtgctgaa gaaatatgtc caggcagcag ttgcaaacag
     3421 ccaggtaaag aagatagaaa gtccaatcag actgaatttt cgttgctcgg atcgatttcc
     3481 ctccaacaat tgtgcatcag cggatgcaag ccaccacaga atcttatctg aggtggaagt
     3541 tttgttttct tccataagat taaaaaagtt gaaaaataaa tttggtaaag gtggcctgtc
     3601 gctctagaga tttaaataga ataacgtaaa catagccgta aatcatattt attacgtata
     3661 aatagtatgt ccctgtcgtt tcaattgtag tgtaatacct tgttatccca tacaatatta
     3721 tagatgcctg caatacaaat tatgtattac aggcattata aagattcttc gtaaatcaca
     3781 gtaattgatt aatcattgca atttgtacaa taaagacctt ttggccccat ttccaaaact
     3841 tcatttttca agaagtgcca attgtcagtt attttttcct tgtcaaattt gacatgttcc
     3901 acaaattcat tcacattttc gtcaaaggaa atttttgtgc ctacggggat cagaagtttt
     3961 atagtgattt cggggttgaa ggctttttta tcattaaata tgagcccggt attggagaaa
     4021 atcaagtcat taccattttg tcttatacca tagattttat ccaattggtc cacagcaatt
     4081 ggtgtatggc tataaaattt aagttttcta ataattcgaa ctttatcgtc catgctttgg
     4141 tcaatattca aattaatctt ggaaaatttg actgagtggc cggttggatt catgatttta
     4201 tcgaaagaaa cagtagttag ttggtcttta gttaaggcga tatccatttc tttttcagta
     4261 gattgaacta cagcgtttgc cggaatatct tcttgactga tatattcata gtcatcagca
     4321 tgcatgttgg cagtaagttc ctcatcggta caatcatcgc aagtcaaacc catctctgac
     4381 atcttatatc ttttaccagg tataaacttc cagccattag gcatttgggc ttcgtcatat
     4441 tttacctcag acaggcgcca ctcaagactt tccggaaatg tcacatattt acctatagga
     4501 atgaaaagat caatctccac ttgctgaaaa cgatatttgc aatcaggtct gatgctgaag
     4561 aatgtaggaa catcgagcgt gtgtgcgtct tttggcgaaa ttgcataact tacgcagtca
     4621 gcattctttt ttgcatcaac tccatcttta ccacgtgaag tcaccctttt ggtggctgac
     4681 catgcaggcc cgttggtagg atagatgtta aatcttacat tatcgtttat ttccagctgg
     4741 ttgtcattgc ccatttccat gtctccaaga accaatcgtc cgtctctatg gtccggagtt
     4801 atcgcggaga tgttaatctg gtcgtctttc agacttccca gatcctgctt cacttcaatc
     4861 gctccataac ttttgaattc tctgaaggtc aatgcactca gtagaataaa gcaaattagg
     4921 ttagaaatcc agaatatagc cagactttga cgaatactgc ggtggacggg ggttttgaag
     4981 aatattttta aagcccagaa aatgacgaac acaacgggaa cggcaaaaat cacgatactg
     5041 ttgagagagg tcaggaaatt aataatagga gaggtggagt taatgaaacc cagaagtgga
     5101 aaagcgagga tataactgac aatcaaagtg atccaaaatc caaggaatat caatgtgaat
     5161 attccaccca gcactagtcc gaaaggtcgt aaaatactta caaggcgggc agtgcctgat
     5221 ctaaaggttt cttccagatc aatggaaggc cggtctgccc taaaagactt ttttccctta
     5281 ttgctaaact gttgtgaaat gtcctgggcg aagtcagtga cctgattgga gaaatgttta
     5341 atttctgttt caagaaattc tgcgatatta tgaatgttga tatcctctcc tttcatggcg
     5401 agatagtccg ccgatgtctt tgcaggggga acaaatatcc acatgatgac ataaagtgga
     5461 atgccaaaac ctcccgagct ggcaagaatg atgaagagca gcctgatcca gttggcattt
     5521 ttaataccta agtaagcagc caatcccgaa caaacgccac ctagtttctt atcggctaag
     5581 ttgcgaaata gtctgcgtgt ccgttgtgtc ccgatcggat caccggaagt gccctgatca
     5641 gagccttcac ccggcaggtc gatagcttca gcaccgaatt cttcaggtct tcccatgatt
     5701 ttgatgatat gttccacatc agctgttgtt acaatagcct tggaagaaac tttatcatgt
     5761 agaagttcac ctagcctgga ttcgatgtcc gacattattt cctgatagct gtcggataca
     5821 gcaaaatgat ttttgatggc gttgaggtaa cgctccataa gcatataggc atcgtcatcc
     5881 attgtgaatg ggaacccgcc gaggttgata tttattgtct tattcatgat cttgattgtt
     5941 tattggttgt ttgagacacg acttttacca gttgattcca gctttccagt aattcaatga
     6001 gaagttcttc tcctgcctga gtaacacgat aatattttct aggaggtccg gtcgtgcttt
     6061 cgcgccaagt atattccaga agtccgccgt tttttagtcg ggtaagtaat gggtatactg
     6121 taccttccac tacaaccagt tcgttttcct tgagtttatt gatgatatcg gaggtataaa
     6181 gttcttcgcc gctaatgatg ccgaggatac acatctctag tacacctttg cgcatttgag
     6241 ctttggtatt ctctgccttc aacgtctcgc gatccggcat ttccttttca ttcatatcct
     6301 tgaataaatt tataagtgaa taaatattgg taacaatgaa taatgtataa tcatgtatat
     6361 caataacgac acaaatatat ggtattatgt attgcttagt actatcttta agataataat
     6421 ttgtaaaaga tagattaatg taatgtaaaa gtcgatgaaa gtaacggtta agcatcaaat
     6481 gaattattgg atagtgtgta atctgttgag atgcgatagc attattgaat gaattggaaa
     6541 gaacgatatt ggggcaggtc gatgtgaact aagtagaaga ataatagctt ataattcaat
     6601 cagggtagta aaatacaaag gcggaaaaaa ctaaggatga aataaaatga aagaaaacaa
     6661 actttggtct actctaatgt aagtctggtg ttggttgtat ggtattactt tcctacctac
     6721 ttcgattttt tgagtgaggg ggtagtaccg ccttcgaagt aggtagataa ttaatagcca
     6781 ccatgcttga cggtattgcc tttcggggcg gccttgccaa aaatcagatg atttgctcgg
     6841 gaattaaatt tttcatttat tcccaaatcc ttgtgtatag cggtttttga attattactt
     6901 tcctacatac tatgaatttt gggagggtga gggtgatgcc acttctgaag taggttgata
     6961 attaatagcc acaatgcttg acagtac
//
LOCUS       DAOQMC010000122         6910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4566_length_6910_cov_5.127061, whole genome shotgun sequence.
ACCESSION   DAOQMC010000122 DAOQMC010000000
VERSION     DAOQMC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4566_length_6910_cov_5.127061"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PKV31_06075"
     CDS             complement(<1..851)
                     /locus_tag="PKV31_06075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB92456.1"
                     /translation="MISAANINEQSGRQILDSLDVIGDYIIEGTLGEGRFGIVYKAKH
                     RNTGHPVAVKLLKVWSVPQHAQMNLIRRFELEFETGQIKSDHLVHTIEMDQIEGIPFI
                     VMDYYPGGNLERKINAGITQDEAIVIAKGILRGLNDLHRNGKIHRDLKPENVLFDDQG
                     RPRLTDFGISGHINIQLTVVNQDDHPEQVFGSYAYMAPEQLSPIKRKNTLLPTIDIFS
                     YGVLCFEMFHNSLPFGPWKSHDDIDPYVRRAASGMTGPIKEDLSPIWKEILIKCLNPD
                     RERRFQNT"
     gene            complement(851..1666)
                     /locus_tag="PKV31_06080"
     CDS             complement(851..1666)
                     /locus_tag="PKV31_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92457.1"
                     /translation="MRCKNCGANNSVHALKCIHCNVPLVGSMVVDMNISDPKISDGII
                     CNNCKSINPPNATNCRQCKAQLANPRFSGFEKSSDQLEIKSESAPPAPQQRKPRRNVC
                     PVCSYPNQPISNECVKCHTPLLSVNPTVPKDEPSKEINTQKQPAPNKIPERKEPDQQA
                     DSLNMTINPWAQQPVIPDEFCLIPLKSDYSASGDPISLVNAENNLNRDKLDPGNMTIT
                     SSSQAIVQQRGGKWLIRDTSSMQTTFVKVQGEMELRDGDIILMGNKIFKFSRK"
     gene            complement(2323..4113)
                     /locus_tag="PKV31_06085"
     CDS             complement(2323..4113)
                     /locus_tag="PKV31_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNB92458.1"
                     /translation="MEIKRLFDFLTHQLERNPDLHFLSVKKTLFDDNKQWKHFSYRDL
                     QDNADKVSRLLLDAGIEKGDKIAIISENRPEWNFVDMGSQQIGVVNVPMYPTISEEDY
                     EFIFRDSGIKFAFVGNDTIYQKVLPLMAKLDGLKGIVTFDKLNGTRWLWDELEQIENP
                     GRQYIDAIKSTIVELDIATIIYTSGTTGNPKGVMLTHQNIVSNLTGVAKVLPFRDGEK
                     SLSFLPLCHSFERTVFYAYLMMNIHIYYAENLETIGDNLKEVQPYCFTTVPRLLEKVY
                     EKIMAKGHELTGIRKSLFFWSVRVGSRYKLGHPGSLIYRLQLRIARKLVFSKWLEALG
                     GRVEFIVTGAAAMQPRLITIFSAAGINVIEGYGLTETSPVLTVNRISERERCLGTIGM
                     PIPGVEIKLAEDGEILARGLNIMKGYFNRPDLTAEVIDSNGWFHTGDIGEWKEHFGNN
                     FLKITDRKKELFKTAGGKYIAPQAIENKMKESRFIEQIMVVGGDDYKYIGALIVPSFV
                     QLNAWAVDMGISTSSNQNLINSPEVLALMLKEVERLNVHFGKWEQIKKIRLLSEEWTI
                     ESGMLTPTLKLKRKFLYARFKEEIADILQG"
     gene            complement(4120..4677)
                     /locus_tag="PKV31_06090"
     CDS             complement(4120..4677)
                     /locus_tag="PKV31_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNB92459.1"
                     /translation="MNKINSVFPTISETMLMVIDVQERLMPVIHDSHRVTHNINTLIR
                     GAAILGVDTLITEQYPKGLGNTIEEIDRSSNPPIFEKDCFSCLLQPNVSDFLFTKQKK
                     HLVLCGVESHICVLKTALDAESRGYTVHLVADAVSSRTPENKDLAIGRMRQSGIYIVS
                     VEMILFMLMERAGTDQFKAISKLIK"
     gene            complement(4696..6426)
                     /locus_tag="PKV31_06095"
     CDS             complement(4696..6426)
                     /locus_tag="PKV31_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNB92460.1"
                     /translation="MNGGEMIARILTKYGVRFLFTLCGGHISPIFVSAERAGIRVIDT
                     RHEVTAVFAADAVSRLTGTVGVAAVTAGPGVTNTISAVKNASLAQSPLILLGGAAATI
                     LKGRGSLQDIDQLSIMKTVCKWSASISRVKDIVPTLEKAFQIAQEGVPGPVFVECPID
                     VLYGEELVRDWYGAKSNQEPAKNFREKALRWYVNFHANRLFRGKDSVDFDKPVVAPIF
                     PIHGTMDVSKAAEMLRKAKKPLLIIGSGAMMQAARAGDLAQAVERLGIPVYLSGMARG
                     LLGRDNKLQMRHKRKEAIKGSDLIILAGVPNDFRLDYGNHIGGRRFISVNRSKTDLFK
                     NKRPTLPILGDPMGFLIALSDYFQAECPEWFGVLQSLNDNREQSIQEQAAAPVTTGMN
                     PLALFRQLENQLDDDTILVADGGDFVATASYILKPRTPLSWLDPGVYGTLGVGAGFAL
                     GAQLVFPEKQVWIIFGDGSAGYSLMEYDTYKRHNLPIVSIIGNDACWSQIARDQVEFL
                     KSDCAMNLAYSDYHIIPRAFEGDGIRVDSLDKWQDAIQVAKDSRRKNIPFILNAIIGK
                     TDFRKGSISV"
BASE COUNT         1957 a         1587 c         1352 g         2014 t
ORIGIN      
        1 gtattttgaa agcgacgttc cctgtccggg ttaaggcatt taatgagaat ttctttccat
       61 atcggagata gatcttcttt tatcgggccg gtcattccac ttgctgcgcg tctgacatat
      121 ggatcaatat cgtcgtgaga cttccagggt ccgaaaggta atgaattgtg aaacatttca
      181 aagcacagga ctccatagga aaagatgtcg attgtgggca acagcgtgtt ttttcttttg
      241 attggtgaaa gttgctcagg tgccatatag gcataactac cgaacacctg ttcggggtga
      301 tcatcctgat tgaccacagt caattgaata ttgatatgcc cggatatgcc aaaatcagtc
      361 agtcgtggac gtccttgatc atcaaaaaga acattttccg gttttaggtc tctatgaatt
      421 tttccattac gatgaaggtc gtttaatccc cggagaatcc ctttggctat aacaatagct
      481 tcatcctgtg taatcccggc attaatcttt ctttcgaggt tgcctccggg ataataatcc
      541 ataacaataa aaggaatacc ttcaatctga tccatttcaa tagtgtgcac cagatgatca
      601 cttttgattt gtcccgtttc aaattccaat tcaaatcttc tgatcaaatt catttgggca
      661 tgttgaggaa ccgaccacac cttcagcaac ttaacagcga ccggatgtcc cgtattccgg
      721 tgttttgctt tatagacgat accgaaccta ccttccccca atgttccttc aatgatgtaa
      781 tctccgataa catcaaggga atccaggatc tggcggcctg attgttcgtt tatattggct
      841 gcggagatca ttatttcctt gaaaatttaa aaatcttatt gcccattaat atgatatctc
      901 cgtcccttaa ttccatctca ccctggactt ttacaaaggt agtctgcatg ctgctagtat
      961 ccctaatgag ccattttcca ccccgttgtt gcacaattgc ctgactggat gaagtgatgg
     1021 tcatgttgcc ggggtcaagt ttgtcccgat taaggttatt ttcagcgttt accaatgaga
     1081 ttgggtcacc tgaagcggaa taatcacttt tcaaaggaat caaacaaaat tcatccggga
     1141 tgacaggttg ttgtgcccaa ggattgatgg tcatgtttaa agaatctgcc tgttgatcag
     1201 gttctttcct ttccgggatc ttatttggag ctggttgctt ttgggtattg atttctttgg
     1261 atggctcatc ctttggcacc gtcggattta cggataacaa tggtgtatga catttgacac
     1321 actcattaga aatgggctga ttcggatagg agcaaacagg acatacattt cgtctgggtt
     1381 ttctttgctg aggagccgga ggtgcacttt ctgacttaat ttctaactgg tccgaagatt
     1441 tttcaaatcc ggaaaaccgg ggattggcca attgtgcctt gcattgccgg cagttggtcg
     1501 catttggggg attgattgat ttgcaattat tacagattat accatcggag attttagggt
     1561 cggaaatatt catatcaacc accattgatc caacaagtgg cacattgcaa tgaatacatt
     1621 tcaaggcatg gacgctatta tttgcaccgc agtttttaca tctcatgata ctataattta
     1681 aaactgaaaa tatcaactcc caaataaaaa agtcaggtaa atgaaattta caaaaatcgt
     1741 ctataagtaa tctgaattgt actgacctta ccaatcaagt attcaggcca aataaaatca
     1801 agctttcaat atatatcccg ccttcctcta attacaagta caatcaagcc aaaatctgca
     1861 agtaaaaata actgatagtt gactaaaagt ggcctttaaa ccatgtattc atgccaaatg
     1921 taatcaaatt ttctcacctt tcattacaga atttaaaatg ttatccggat gtactttcct
     1981 tcctgcttga taaaatgtca gagtttcctg acaggtaaat tgggagtgta ctgtaaaatt
     2041 gccaattcaa catcaaaaac atcttgtcaa tcagatttat tgatcattag ttgcccacat
     2101 attaggcagg agaataataa tccaccaaca tcgggaatca gctcatttca aagacgtgaa
     2161 aaaaattgct ctttggtaag gccatctttt aaattatctt cttcccagcg aatcaatagt
     2221 taaattattg attcaaaatg acttacctgc ttccgtagtc ctaacagacg gtctaaaaca
     2281 atcaccgtag aaatttgata ccattgcaga agcaacatct catcaacctt gcaatatatc
     2341 agctatttcc tctttaaacc tggcataaag aaacttgcgt ttcagcttaa gggtaggtgt
     2401 aagcataccg gattcgatgg tccactcttc agaaaggagg cggatttttt taatttgctc
     2461 ccacttgccg aagtggacat tgagccgttc gacttccttt aacatcagtg ccaacacttc
     2521 aggactattt atcaaattct gattggaaga tgtgctgata cccatatcaa ccgcccaggc
     2581 atttagttgt acaaaggaag gcacgatcaa tgcaccgata tatttgtaat cgtcaccgcc
     2641 gacaaccatg atttgttcga tgaagcgact ttccttcatc ttattctcaa tcgcttgcgg
     2701 tgcgatatac ttaccacctg ccgtcttgaa aagttctttt tttctatccg taatcttgag
     2761 aaaattatta ccaaaatgct ctttccactc accgatatcg cctgtatgaa accaaccatt
     2821 gctatcgatc acttcagctg tgaggtcggg acgattaaaa tatcctttca tgatgttgag
     2881 gcctctggcc aggatctcac catcctctgc caattttatt tccacaccgg gaatcggcat
     2941 accgatggtc cccaggcacc gctcacgttc ggagatgcgg ttgacagtta gaacaggaga
     3001 cgtctcagtc aagccatagc cttcaatcac attaatcccg gcggcactga agatcgtaat
     3061 gagtcgcggt tgcatcgctg cggcaccggt cactatgaat tctacacgtc cgcccaaggc
     3121 ttcgagccat tttgaaaaca ccagtttccg cgcaattcgt aattgcaacc gataaatcaa
     3181 actccccgga tgaccaagct tataccggct cccaacccta acgctccaga aaaacaaact
     3241 cttcctgatg cctgtcagct cgtgaccttt ggccatgatc ttctcataga ctttctcaag
     3301 taaacgcggc acagtagtaa agcaataagg ttgaacttcc ttcagattgt cacctatggt
     3361 ttcgaggttt tcggcataat aaatatggat attcatcatc aggtaagcgt aaaataccgt
     3421 acgctcaaaa ctgtgacaca agggcaggaa gctgagagat ttttcaccgt ccctgaatgg
     3481 caataccttg gcaacgccag taaggttgct tacgatattt tgatgcgtga gcataactcc
     3541 cttgggattg ccggtggtgc ctgaggtgta tatgatagtt gctatgtcca gctcgactat
     3601 agttgatttg atggcgtcaa tatattgcct accgggattc tctatttgct ccaattcatc
     3661 ccaaagccat cgggtgccat ttaatttatc aaaagtcaca atgcccttca acccatccaa
     3721 cttggccatt aaaggtaata ccttctgata aatggtgtca tttccaacaa atgcaaattt
     3781 tatgccagaa tccctgaaga taaactcgta atcttcttcg gaaattgtag ggtacatagg
     3841 cacattgacg acacctatct gttgagatcc catatccaca aaattccatt ccggtcggtt
     3901 ttcactgatg atggcaatct tatcgccctt ctcaattcct gcatcgagca acaaccggct
     3961 aaccttatct gcattgtcct gcaaatctct ataggaaaaa tgtttccatt gcttattatc
     4021 atcaaagagt gtcttcttta cagaaagaaa atgtaaatca ggattccttt caagttgatg
     4081 agtaagaaag tcaaataatc gttttatctc catgatttat tatttgatga gtttgctaat
     4141 agctttgaat tgatcagtac ctgctctttc cataagcata aaaaggatca tttcaacgga
     4201 aacgatgtat atgcctgact ggcgcatccg tcctattgcc agatccttat tttccggtgt
     4261 acgcgaagaa acagcatcgg caaccaaatg cacggtatat ccccgggatt ccgcatcaag
     4321 agcggttttt agcacgcata tatggctttc gacaccacaa aggacgagat gctttttctg
     4381 ctttgtgaac aaaaaatcgc ttacattggg ctgcaacaaa caactaaagc aatctttttc
     4441 gaagatgggc gggttcgacg atctatcaat ttcttctatg gtattgccga ggcctttcgg
     4501 gtattgttcc gtgatcaagg tatctacacc gagaattgct gcccctctaa tcagggtgtt
     4561 aatattgtgg gtgacacgat gagaatcgtg aatgacaggc attagtcgct cctgaacatc
     4621 aatcaccatc aacatggttt cactgatagt tgggaatact gaattgatct tgttcattat
     4681 ttaattttta aaaaatcaca cgctgattga gcccttacga aagtctgttt ttccgatgat
     4741 tgcatttaaa ataaatggta tgttttttcg cctactatcc ttcgctacct gaatagcgtc
     4801 ctgccatttg tccaatgaat cgacccgaat accatcgcct tcaaatgcac gggggatgat
     4861 gtgataatcg ctatatgcca gattcatagc gcagtcactc ttgaggaatt ccacctgatc
     4921 cctggctatc tggctccagc aggcatcgtt gccaataatc gagacaatcg gcaaattatg
     4981 tcttttgtaa gtatcatatt ccatcaagct gtatcccgca cttccatctc caaagatgat
     5041 ccacacctgc ttttcgggaa aaaccaactg ggcgcccaag gcaaaaccgg ctcccacgcc
     5101 taaggtacca tagacacccg gatccagcca gcttaacggt gtgcgaggtt taagtatata
     5161 ggatgccgta gcaacaaagt cacctccatc agcgacaaga atggtatcat catccaattg
     5221 gttttcaagt tgacgaaaca aggccaatgg attcattccg gtagtgacgg gtgcggcagc
     5281 ctgctcctga atggattgct cccgattatc gttgagcgat tgtaacactc caaaccattc
     5341 aggacactct gcctggaaat aatcagacag ggcaatcaaa aagcccattg gatcaccaag
     5401 aataggcaat gtcggccgtt tattcttaaa caagtcggtc ttgcttcggt tgacagagat
     5461 gaatctgcgt cctccgatgt gatttccata gtccagtctg aaatcattcg ggacaccggc
     5521 aagaataatc aggtcggagc ccttaatggc ttccttcctc ttatgccgca tttgcaattt
     5581 attgtctctg ccaagcaagc ctcgggccat accgctcaga tagaccggga tacccagacg
     5641 ttcaacagcc tgtgccagat cacctgccct ggcagcctgc atcatcgctc ctgagccaat
     5701 gataaggagt ggtttttttg cttttctcaa catctcagcc gccttactca catccattgt
     5761 accatgaata ggaaaaatgg gagcaacaac cggcttgtca aagtcgaccg aatcttttcc
     5821 tctaaaaagc ctgttggcat ggaagttgac gtaccaacgc aaggcttttt ccctaaaatt
     5881 tttagccggc tcctggttac tctttgcacc ataccaatcc cgaaccagtt cttcaccgta
     5941 caatacatca atcgggcact ctacgaaaac aggaccggga acgccttcct gtgctatttg
     6001 aaaagctttt tcgagggtcg gcacaatatc tttaaccctg gaaatactgg ctgaccattt
     6061 gcacaccgtt ttcatgatgg atagttgatc tatatcctga aggctgcctc gcccctttag
     6121 aatcgttgct gcagcaccgc ccagtaaaat gagcggtgac tgggcaagtg aagcattctt
     6181 gacagcagaa atggtgttgg tgacaccggg ccccgcagtc acagcagcaa cgccgacagt
     6241 accggtcagt ctcgaaactg catcggcagc aaaaacggca gtaacttcat gtctggtatc
     6301 aatgacacgg attcccgctc tttcagcact cacaaagatt ggcgaaatgt gaccgccaca
     6361 caaagtaaaa agaaaccgta caccatactt tgtcaaaatg cgggcaatca tttctcctcc
     6421 attcataaaa atcagcatta gttaatatct acacaaaccg tgaaaatagt caattttgta
     6481 aaatgttaag tgataaatta ccatgtgcgt taatagccat agcagatatc atcagcaatg
     6541 acgaaagata gagtatccga ataaagatta caaattgaat tggcgaaaga ttattgataa
     6601 tctgcttttt atacattttg catctttcct caatatgaat aaggaaaaaa tgtagtcttt
     6661 ctaaaactcg atgtactgct cctacaatag atagctcaaa atacttactg ctcattattt
     6721 ttagcttcct tttggtggtt tgaaggaatg gtgtcctact actttgaata aacagcgatt
     6781 aaatgtggga tagcacctgc tactgacaaa atttagttct tgactattta tgagcctctc
     6841 atcacatcat cctctgtaaa atgcgaagtc attatctcca gtgtacgctc agcactgaaa
     6901 gtacccaaca
//
LOCUS       DAOQMC010000123         6881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4591_length_6881_cov_4.505274, whole genome shotgun sequence.
ACCESSION   DAOQMC010000123 DAOQMC010000000
VERSION     DAOQMC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4591_length_6881_cov_4.505274"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2331
                     /locus_tag="PKV31_06100"
     CDS             <1..2331
                     /locus_tag="PKV31_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNB92461.1"
                     /translation="FNTIIWITFTLASYVEVQAQTMDSLYHAVEVDLSTTSIQEIARL
                     GIEADHGIYAPGRFWINYLSDYEIGLLQSNKISFRRIDRSGMKVFDRSGDNCFNTNPN
                     KSDPRNFRLGKMAGYYTLEEMNAILDSMHLLFPQLISTRKDISTFRTFEDRPIQWLRI
                     SNHPEKDQEKPRVLYTALHHAREPMSLTQLIYFMWYLLEEYANNEEIRELIDNTELYF
                     IPCVNPDGYAFNASNKPEGGGMWRKNRWLHPSGFIGVDLNRNYGLAWGIDDVGSSPDP
                     FSSVYRGPGPFSEPETQAVAYLTDQYKFDLVLNSHTYGNYIIYPWNYTADPCPDDEGF
                     KAIARVYAGRNSFAIGNSKETVGYLSNGGSDDWIYAHDPEHKIYSFTPEVGFNSEGFW
                     PDASRIRQISGNSLEGNIVFAEMAHRYFDIAFLDPLFLIRNKDNEINFSISRAGSGQG
                     PITLKFRILDNSAILDKNRLDYDMPVPGLRVDRIVITPGTTAMNGDPVKLEITREMGL
                     LTVKDTVEFTVKSGISTIINKCDDISTIIPDGVDWGEDYKEFYSPPSSFADSPDGFYR
                     ENQNSGIVFEREFYIPTDKNTLLTYWLKWDIEGNFDYAQVYAITDFGEVPLCGKFTRP
                     GTIFQKEGQPLYDGNSVIWVREEVDMSEFSGQYIHIGARLVSDDQENRQGINLDDLEL
                     ISYSDISGKSVLVNPSEVAIYPNPTSDKFKILVPYKNLMADVVVRDNLGRTITSIRNC
                     NLSEYEFHIEGYARGIYYISIYTDNRNLFTKKLNKL"
     gene            2342..2830
                     /locus_tag="PKV31_06105"
     CDS             2342..2830
                     /locus_tag="PKV31_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNB92462.1"
                     /translation="MKKLFTVIILAILILGAYLAYLIYFGTTKPEFVQVDKISVSDVK
                     LLPNPSFDLAAHLEFNNPNSIGVHIDSVICDIYIEESKVATVVSKEKVEAPANAHFFI
                     PVNTRIDLSEQEYAKIFGNNLFESILNNTIKLRFKGEMTLTKFGLHRKIPFDYDYKYK
                     IL"
     gene            2884..3744
                     /locus_tag="PKV31_06110"
     CDS             2884..3744
                     /locus_tag="PKV31_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HNB92463.1"
                     /translation="MPNPIAGASRKTTRPPVKKRRVKPQKTTSGRLSEKKVIFGTAFL
                     VLLCMISIHLSGCFDELKASKINKSYIKFPEFGIHMPTLYSINGIDVSVHQGMLDWKA
                     IKNAAAGSVKIDFAFIKATEGRTKVDKYFKYNWEQAEKNGLIRGAYHFFLPNKSGMDQ
                     ARLFLKTVDFKTGDFIPVCDIEITGGADSDMIEQNLRDWLDTVEKEIGKKPILYSSKK
                     FYETYLHQEFSDYPLWIAHYNVPALELKDQNRWLFWQHNDKGKVKGVRKHLDFNVFNG
                     TMDELNLLRL"
     gene            3841..5187
                     /locus_tag="PKV31_06115"
     CDS             3841..5187
                     /locus_tag="PKV31_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNB92464.1"
                     /translation="MNIHLIAIGGAIMHNLAMELCDNGHIVTGSDDAIYDPALTRLKE
                     KGILPEAMGWYPERIHAGLDLVILGMHARADNPELKKAQEEGIKVMSFPEFMYEHSKD
                     KKRVIIAGSHGKTTTTSMVLHLLRSAGKEVDYLVGAQLEGFGNMVRLSDAPIAVFEGD
                     EYTASPLDLRPKFMHYKPHISVITGIAWDHINVFETHEKYMHQFELLIEDTLSRGTLV
                     YYGGDKDLKKLASKYPGKKIEYEALPYKSDEDGWSVMVGDEAIPVSIFGRHNFENLCA
                     AYEVVKELGLTNQQFISGLRSMPGPARRLEVLRKADKKTIYYDFAHAPSKVDATVKAV
                     RERHPGQQLIAFLELHTFSSLNRDFIPQYAHTLDAADEAIIFYNPDTVKAKHMDIVPP
                     DYIVHSFKRDDIDIIENTASLHQRLLESVQKEAVVLIMTSGNFGGLDIRKEFSDKE"
     gene            complement(5232..6104)
                     /locus_tag="PKV31_06120"
     CDS             complement(5232..6104)
                     /locus_tag="PKV31_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNB92465.1"
                     /translation="MDNKEKKIKLIECPRDAMQGLHEFIPTVSKIEYINQLMRVGYDT
                     LDCGSFVSPKAIPQMADTEDVLQGIDMSLSPTKLLVIIANVRGALQAAEQEKVTYLGY
                     PFSISETFQLRNTNATIMESLIRVEDILEICVKKGKKLVLYLSMGFGNPYGDPWNLEI
                     AENWVDRLYKMGIETMSLSDTIGVSSPDSIEYLFSGLIPQYPGVEFGAHLHTHPAKWY
                     EKVDASFSHGCMRMDSAIRGFGGCPMAKDDLVGNMATENVIQYLIEKNRLPGIDLEQF
                     AIAITMADRIFPKH"
BASE COUNT         2187 a         1427 c         1455 g         1812 t
ORIGIN      
        1 ttcaatacaa taatctggat aacattcaca ttagcttcct atgtagaagt acaggcccag
       61 acaatggatt cgctttatca tgccgtggag gtggatttaa gtacgacatc tattcaggaa
      121 attgcccgtt tggggataga agctgatcat ggtatttatg ctccgggccg attttggatc
      181 aattatttgt cagactacga aatcgggctg ttacaaagca ataaaatatc gttccgaaga
      241 atagaccgtt ccggaatgaa ggtttttgac agaagtggag ataattgctt taataccaat
      301 cccaacaaat ccgatcccag gaatttccga ttgggtaaga tggccggata ttatacactc
      361 gaggaaatga atgccatcct ggacagtatg catcttttat tcccacaact tatttcgacc
      421 agaaaagaca tatcaacatt cagaaccttt gaagacaggc caatacaatg gctccgcata
      481 tctaatcatc ccgaaaaaga ccaggaaaag ccccgcgtcc tatacacggc actccatcat
      541 gctagggagc cgatgagcct gacccagctt atttatttta tgtggtacct tttggaggaa
      601 tatgccaaca atgaagaaat aagggaatta atagacaata cggaattata cttcattccc
      661 tgtgtcaatc cggacgggta tgccttcaat gcaagtaaca aaccggaagg gggaggtatg
      721 tggcgtaaaa acagatggtt gcatccttca ggattcattg gtgtagatct caaccgcaat
      781 tatgggcttg cctggggcat tgatgatgtc ggctcaagtc cggacccatt cagtagtgtg
      841 taccggggac cgggtccatt ttctgagccg gagacacagg cagtagccta tctgactgat
      901 caatacaagt ttgaccttgt gctcaatagc cacacttatg gcaattacat catctatccg
      961 tggaactata ctgccgatcc atgtcccgac gatgaggggt tcaaagctat tgcaagggta
     1021 tatgccggaa gaaacagttt tgccataggt aacagcaagg aaaccgtcgg atatctttca
     1081 aacggaggat ctgatgactg gatatatgcg catgatcccg aacacaagat atattcattt
     1141 actccggaag taggattcaa tagtgaagga ttctggccgg atgcctcgcg aatcaggcag
     1201 atttccggaa acagtcttga agggaatatt gtttttgctg agatggcgca tagatatttc
     1261 gacattgctt ttcttgaccc gctctttctg attcgtaaca aggataatga aatcaatttt
     1321 tcgataagcc gtgccggttc cggacaggga ccgattactc taaaatttcg gatactggac
     1381 aattcggcaa ttctggacaa aaaccggttg gactacgata tgcctgtccc gggcctgcgg
     1441 gtggacagaa tagtcatcac tcccggcaca acagcaatga atggcgatcc ggttaagttg
     1501 gagatcacca gagaaatggg tctgctcacc gtaaaggata ctgtagaatt taccgtcaag
     1561 tcagggattt cgactatcat caataaatgc gatgacatat caaccatcat tccggatgga
     1621 gtggattggg gagaggatta caaggaattt tactcacccc cgtcttcatt tgcagattct
     1681 ccggatggat tctaccgtga aaatcaaaat tccggcattg tgtttgaaag ggaattctat
     1741 attccgacag ataaaaacac cttgctgaca tactggctta aatgggacat agaaggaaat
     1801 ttcgattacg cacaggtgta tgccatcaca gactttggag aagtgccact ttgcgggaaa
     1861 ttcaccaggc cgggtacaat attccaaaaa gaaggacagc ctctgtatga tggcaatagt
     1921 gtcatctggg tgcgtgaaga agtcgatatg agtgaatttt ccggtcaata tattcatatt
     1981 ggcgcaagac tggtgagtga tgaccaggag aacagacagg gtatcaatct ggatgatctc
     2041 gagcttattt catacagtga tatttccggt aaatccgtat tggtcaatcc gtccgaggtt
     2101 gcaatctacc ctaaccctac ctctgacaaa ttcaaaattt tagttccata taaaaacctg
     2161 atggctgatg tggttgtccg ggacaatctt ggcaggacaa taacatccat cagaaattgc
     2221 aacctgtcgg aatatgaatt ccatatcgaa ggatatgcgc gcggaattta ttatatcagt
     2281 atctatactg acaatcgcaa tttatttacc aaaaaactga acaaactata atttagacac
     2341 catgaagaaa ttatttactg tcattatctt agccatattg attctgggtg cttatttagc
     2401 atatctcatt tacttcggca cgacaaaacc tgaatttgtg caagtggata aaatctcagt
     2461 cagtgatgta aaattattac ctaacccatc ctttgatctg gcggctcacc tggaattcaa
     2521 caaccccaat tccattggag tccatataga tagtgtcatt tgcgatattt acatagaaga
     2581 aagtaaagta gcaactgtgg tcagtaaaga gaaggtcgag gctcccgcta atgcccattt
     2641 tttcatacca gtcaatacaa ggatagatct gtcagagcag gaatatgcaa aaatattcgg
     2701 gaataatctc tttgaaagta tcctgaacaa tacaatcaaa ttgagattca agggtgaaat
     2761 gaccttgact aaattcggct tacaccgaaa gattccattc gattatgatt ataagtataa
     2821 aattctttaa aaagaatcat attattaatt taattaatat atttgcactt aaataataat
     2881 ggaatgccaa atcctatagc cggggcatcg cgaaaaacca ctcggcctcc tgtaaaaaaa
     2941 agacgggtca agccgcaaaa gaccacatct ggaagacttt ccgagaaaaa agtgatattc
     3001 ggaactgcat ttcttgtact tctatgcatg ataagtattc acctttccgg atgttttgat
     3061 gaattaaaag cttccaaaat caataaaagc tatataaagt ttccggaatt cggtatccat
     3121 atgcctacac tgtattccat caatggcatt gatgtttcgg tgcaccaggg aatgcttgac
     3181 tggaaggcca taaaaaatgc agcagccggc tcggttaaaa tagacttcgc atttatcaag
     3241 gctacagagg gaagaaccaa ggttgacaaa tattttaagt ataactggga gcaagccgaa
     3301 aaaaacggat tgatcagggg ggcttatcat ttttttctac caaataaaag tggaatggat
     3361 caggccaggt tatttctcaa aaccgttgat tttaaaaccg gggattttat tcctgtatgt
     3421 gatattgaga taacaggcgg tgcagattca gacatgatag aacagaatct cagggactgg
     3481 cttgacacgg tagagaaaga aatcggcaaa aaaccaatcc tgtatagttc caagaaattt
     3541 tatgaaacat acctacatca agaattttcg gattatccat tatggattgc tcattataac
     3601 gtgcccgccc ttgaactcaa ggaccaaaac agatggcttt tttggcaaca taacgacaag
     3661 ggcaaggtaa aaggagtcag aaaacatctg gatttcaatg ttttcaatgg tacgatggat
     3721 gaattgaacc ttttaaggtt gtaagtgtga agaaaaacca tgaagatgct tgtcaactct
     3781 gcaatccggc ataaattcgg tttttatgag ccaaacgtga attaaaaaaa taataacaac
     3841 ttgaacatac atcttatagc tatagggggt gccatcatgc acaatcttgc catggaatta
     3901 tgtgacaatg gccacattgt caccggtagc gatgatgcaa tctatgatcc ggcactcacc
     3961 agactcaagg aaaaaggaat tctccctgaa gcaatgggtt ggtatccgga gcgaattcac
     4021 gccggactcg acctcgtcat cctgggaatg catgcccgtg ctgacaatcc tgaattgaaa
     4081 aaagctcagg aagagggaat caaggtaatg tccttcccgg aattcatgta tgagcattct
     4141 aaagataaaa agcgcgtaat cattgccgga agtcatggca agactacaac tacttccatg
     4201 gtactgcacc tgctgcgatc tgccggcaag gaagtggatt atttggtcgg cgcacaactg
     4261 gaaggatttg gcaatatggt gcggctttct gatgccccta ttgcagtctt tgaaggagac
     4321 gaatatactg catcccctct ggacctcagg ccaaagttta tgcattacaa accgcatatt
     4381 tcagtgatca caggtatagc ttgggaccat attaatgtat ttgagaccca tgaaaaatac
     4441 atgcatcagt tcgagctact catcgaagat acactcagca gaggcacatt ggtgtattac
     4501 ggaggcgaca aggacctgaa aaaacttgca agtaaatatc ccggtaagaa aatagaatac
     4561 gaagccttgc cctacaagag tgatgaagac ggatggtcag taatggtggg ggatgaagct
     4621 attccggtat ccatctttgg aaggcacaac ttcgaaaact tatgtgcagc atatgaagta
     4681 gtgaaagagc tcggtctgac caatcaacaa tttatcagcg gattgagatc aatgcccggt
     4741 ccggctcgtc ggctggaagt ccttagaaag gctgacaaaa aaaccattta ttacgacttc
     4801 gcacacgccc catccaaggt agacgccacg gtgaaggcag tcagggagcg ccatcccgga
     4861 cagcaactaa tcgcctttct agaacttcat actttttcca gcctcaacag ggacttcata
     4921 ccacaatatg cccacacgct cgacgcagca gacgaagcca tcatcttcta caaccctgat
     4981 accgtaaagg caaaacatat ggatatagtg ccaccggatt atatcgtcca cagcttcaaa
     5041 cgagatgata tcgatatcat cgaaaataca gccagtctgc atcagcgatt gttagagtcg
     5101 gtgcagaaag aagccgttgt cttaatcatg acgagtggaa actttggcgg attggatata
     5161 cggaaggagt tctctgacaa agagtgattg tgcttgagtc agtccaactt tttactttaa
     5221 atctttacca atcaatgctt tgggaaaatt cggtctgcca tagtgattgc gatggcaaac
     5281 tgctccaagt cgatccccgg cagtcggttc ttttcgatta aatattgaat tacattttct
     5341 gtagccatat tacccaccaa atcatccttt gccatggggc atcctccgaa gcctctgatc
     5401 gctgaatcca tacgcatgca tccatgagag aaactcgcat ccactttttc gtaccatttg
     5461 gctggatgtg tatgaagatg ggcaccgaat tccacacccg gatattgggg gatcaggcct
     5521 gaaaaaagat attcaatact gtccggacta cttacaccaa tggtatctga caaagacatc
     5581 gtctcaatac ccatcttgta taacctgtct acccagtttt cagcaatctc aaggttccat
     5641 ggatcgccat aaggattacc aaagcccata ctgagataga gcactaattt tttacctttt
     5701 tttacacaga tctccagaat gtcctccacc ctaatcaatg actccataat ggtagcattg
     5761 gtattacgga gctggaaggt ttcagaaata ctaaaaggat aacccagata ggtaactttt
     5821 tcctgctctg cagcctgaag ggcaccgcgc acattggcga tgatcaccaa caatttggtg
     5881 ggtgaaagac tcatatcgat accctgcaga acatcctcgg tatcagccat ctgcgggatg
     5941 gccttgggag aaacaaagct accgcagtcc aaagtatcat agcccacacg catcaactga
     6001 ttgatgtatt caattttgga aaccgtggga ataaattcat gcaacccctg catagcatcc
     6061 cgtggacact caattaattt aatctttttc tctttgttat ccatataccg tccggtacaa
     6121 atgtaagaaa cttcagcttt aaagaaggat ataatccgga ttaagaaatc aaaacagaaa
     6181 gaaatcaaaa gaaaacccat ctataaagtc accggaattt tcaaaaacaa ggcattatat
     6241 acctgaaaaa caaaaataag gattgaagta catttcttga aagaccctac tcgttttaat
     6301 agatgataag ttattccgtc agcattggaa tattagccaa tcctgcttcc tggctaaact
     6361 ttaagatacc tcacaaagat ctctctgaat atccccggaa attcaagaat agtatgacaa
     6421 cgatctcggc tcgtatatct gactttgaaa atacatcttt ggacaaatac atctgagtgc
     6481 aatcaatcaa tacagaaaaa gattgactta ttgaagaatt gtggtcgtaa aaagcgttca
     6541 ttgctaaaaa atacccgctg aattttttcc aatacagagc ctgaaataag atgggagcga
     6601 agaactgatt gaattcattc ggacatggta aaatgaaaaa tttgacggag aatgaaaaaa
     6661 taataaatgc gcctaaaatg aatgggtgtg gtgggggcaa gggtttcgat gggaaaaatc
     6721 gaagtaggta ggaaaataat actccacgat ctgcgtgaca ccagcatccc actaaaaaag
     6781 caaatattta tatgtctata aatggaaaag ccatttactg cacaattcga aaaaggcttg
     6841 tcccttgaat ataccgctat taatcttcta cctaattcct g
//
LOCUS       DAOQMC010000124         6667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4809_length_6667_cov_4.748488, whole genome shotgun sequence.
ACCESSION   DAOQMC010000124 DAOQMC010000000
VERSION     DAOQMC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_6667_cov_4.748488"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKV31_06125"
     CDS             <1..486
                     /locus_tag="PKV31_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNB92466.1"
                     /translation="MCFYARLGDGAYTLKLLKALLKPAASGGGTYDNLFDAHPPYQID
                     GNFGATAAIAEMFVQSHGREEIIRLLPALPDDKDWANGRISGLRARGNYEISLEWNDH
                     VLLNAVILAHNDGQCRIVLPENMIIYDEAGTEIPLTMTKDCFSFSVKAGKKYFVKNSL
                     R"
     gene            642..1253
                     /locus_tag="PKV31_06130"
     CDS             642..1253
                     /locus_tag="PKV31_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNB92467.1"
                     /translation="MMKLLNILILNLIALATMVAQTPVQIKVDGMTCDFCVNSVTKKL
                     SNAKEIENITVDLEKGLVHFNVKKGSKVDKKKYMELVEKAGYDAREIKVGNEIKATQN
                     AKAKQVNYVPASHETTTFKVGGECGMCKQRIESNVRAIKGVKSASWNKETKVLTVTYN
                     PMKIDIMDVHKKIASVGHDTELVKADDAVYDKLPNCCHYDRLK"
     gene            complement(1417..2349)
                     /locus_tag="PKV31_06135"
     CDS             complement(1417..2349)
                     /locus_tag="PKV31_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNB92468.1"
                     /translation="MSIPMIILLVFAALFVFSGLVTVRQGTVAVITMFGKYQRILTPG
                     LNFKIPFLEQVYKTISIQNRSIELEFHATTSDQANVHFTSMLLYAVLNNSEETVKAVA
                     FKFIDERSFMQTLSRVVEGNIRAFVATKKQSEVLSLRREIVEVVKGNVDAMLEEWGYH
                     LIDLQINDISFDEEVMRSMAKVVASNNLKAAAENEGQALLITKTKSAEAEGNAIKIAA
                     EAEREASRMRGQAVASFREEVARGMGQASREMHEANLDTSVILFTMWTESIKQIAENG
                     KGNVIFLDGSVEGMEKSIKNLMAINQLDLMEKRK"
     gene            complement(2441..3325)
                     /locus_tag="PKV31_06140"
     CDS             complement(2441..3325)
                     /locus_tag="PKV31_06140"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNB92469.1"
                     /translation="MHFEPSEMATKDLHQLLLGIVAPRPIAFVSTLDEHGIPNLAPFS
                     FFNCFSSNPPIVIFSANRRVSDNTTKDTLANVKASGECVINAVSYHVVRQVTLCSVDY
                     PSEVNEFEKAGLTPIPSDLVKPPRVKECLAHLECKVKDILPLGEGGGAGNLVICEVVR
                     IHADSSIMDENHRIDPNKIDLMARMGRAFYSRASGDAVHTIVQPYEASPIGFERLPEF
                     FKSSHILTANDLASLASIEDVPGDEDLAAFTNREEIRSLLAQQEASALLQQYAHRVLA
                     EDRKKEGFMAMHLAANRE"
     gene            complement(3338..3706)
                     /locus_tag="PKV31_06145"
     CDS             complement(3338..3706)
                     /locus_tag="PKV31_06145"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNB92470.1"
                     /translation="MILISGFEKLSLHAPVGLYPEEKVLGNDFLMDIEVAMDVPDGFL
                     IEKLENTVDYSQLEKIARENFTKGSDLMETGAQMVIEAIQSEFFGILGIRIKVRKVHP
                     ITLPSVDNTFVELKTGYFAK"
     gene            3800..4573
                     /locus_tag="PKV31_06150"
     CDS             3800..4573
                     /locus_tag="PKV31_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB92471.1"
                     /translation="MPVLRSIYNTLLSLLAYFRIFCCVLFLVIAVPFLHLETMIFPNK
                     EKIGFRYRKWFTSLYLFILNIKVKKSGEIAAHNALIVCNHRMMIDPVIVLDYCPGYIV
                     SKAEVASYPILGHGARNAGVIFVRRDQKESRNAIKEKIGELLSEGKNVVLFPEGTVNT
                     DRLTARFSVGSFEEAAKYGRPVIPVALDYKDTSVYWGANISLIQHFLQVFRKPRLETR
                     LVIGSPIKGNDGFELMESAKNFIDNQLEVIRRDWETSQI"
     gene            4612..6084
                     /locus_tag="PKV31_06155"
     CDS             4612..6084
                     /locus_tag="PKV31_06155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB92472.1"
                     /translation="MMHDRKYTALIVVALVGILLFLHRGHFNLELRGTHVWRQSITAL
                     HIRNFVRYDNNILNPRVASFNGGRDNISRYEFPLMQWTIAQVERVTGESVAVVKFYQF
                     LIAISFLIGFMFLLKRAGFDRRFCWMGIWAVAFSPVIYYYSVSPIPDMLALSFATWAL
                     YHAIGFFRSEQNKYWPIFNIGLLISLATLVKLPYILFMGPAVYLIIAKYRMRWKSYFV
                     PFFILAACMIPAFLWYAWVIPQWTVGITKGMFDNQISMDHALQIISYHLKVMFPYRLL
                     NVVFVPFFLLSVIPMFRSIRRDAVLQSMLLGFLAYLAYFLFELNMIDVVHDYYMMPFV
                     PFLFWGVMTGIRFCWQLRYMAAKVIVGIALIVAPFTCLENTQKDWEITHFHDIYEKQA
                     EFIGAAPPNEKVIMLKDFSGFILPYLIDHHGHIFNFDTLPRSWVKNMISEYDVHYLYS
                     NSRTSDSINRDFIRDTVFKHGEFKVFRLKDKTAVDTIPAE"
     gene            6379..>6667
                     /locus_tag="PKV31_06160"
     CDS             6379..>6667
                     /locus_tag="PKV31_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNB92473.1"
                     /translation="MFLNTHSWFSLRYGTLSPERLADTARSLGIRAMALTDINNTSCA
                     WQYIEACRRNDVRPILGIEFRRDDGRMLYVGIARNNTGWANLCRMLSEVSLD"
BASE COUNT         1794 a         1439 c         1432 g         2002 t
ORIGIN      
        1 atgtgctttt atgcgcgact cggcgatgga gcgtataccc tgaagttgtt gaaggcatta
       61 ctgaaacctg ctgcctccgg aggagggact tacgacaatc ttttcgatgc acacccgcca
      121 taccagatcg acggcaactt tggagctacc gctgccattg ctgagatgtt tgtgcagagt
      181 cacggtagag aagagatcat ccgcctgcta cccgctttgc ccgatgacaa ggactgggcc
      241 aatggaagga taagtgggct gagggccagg ggcaactacg aaatcagcct tgagtggaac
      301 gatcatgtcc tgctcaatgc tgttattctg gctcataatg atgggcaatg ccgcatcgtt
      361 ttgcctgaaa atatgatcat ctacgatgaa gcaggaaccg aaatcccgct aacaatgaca
      421 aaagactgtt tctcattttc agtaaaggca ggaaaaaaat attttgttaa aaatagcctg
      481 agataagcca tttaaccaat atttatcaaa agcgacatat ttatgttcta attttgtgcg
      541 ttcaatctcc ggaatgaaac ctttggcaaa atattttgtt gtaattaaag aaaatttgcg
      601 ttttttcaaa ataatatttt taactactca aactttattc aatgatgaaa ttactgaata
      661 tacttatcct caacctgatc gccttagcga ctatggtggc acagactcct gtacaaatta
      721 aggtagatgg catgacctgt gatttttgtg tcaatagtgt gaccaagaaa ctttccaatg
      781 caaaggaaat agagaacatt actgtggatc ttgaaaaggg gcttgtacat ttcaatgtca
      841 agaaagggtc gaaagtcgat aagaagaaat acatggaatt ggtggaaaaa gccggctatg
      901 atgcccggga gattaaggtg ggaaatgaga tcaaggcaac ccagaatgcc aaggcaaaac
      961 aagtcaatta cgtacctgcc tctcatgaaa caactacctt caaggtgggt ggagaatgcg
     1021 gaatgtgcaa acaacgaatt gaatccaatg tgagggcgat aaaaggtgta aaatcagcat
     1081 cctggaacaa ggagacaaaa gttttgactg taacttataa tccaatgaag atcgatatca
     1141 tggacgtaca caagaaaatc gcttctgtgg ggcacgatac ggaattggtc aaagcagatg
     1201 atgctgtata tgacaagttg cctaattgtt gccactacga tagattgaag taaactattt
     1261 gcttcagaaa tcaaaaggag gaccgacttg cttcgatcct cctttttttt gttccgacat
     1321 cctcattgca tgaccggaat ttaacacaat ttattttttc acaggaatgg agggaatcag
     1381 agttccgagt ctccatggtc tcacgattcc tgtttgttat tttctctttt ccatcaaatc
     1441 aagctgattg atagccatca ggtttttaat ggacttttcc ataccttcca cactgccatc
     1501 taagaagatg acattgccct tgccattctc ggcgatctgt ttgatggatt ctgtccacat
     1561 ggtaaagagt attaccgatg tatccagatt ggcttcatgc atctcgcggc tggcttgacc
     1621 catacctcgg gccacttcct cccggaatga agccactgcc tgaccacgca tcctggaagc
     1681 ttccctttcg gcttcagcag caattttgat ggcattacct tcagcttctg ctgacttggt
     1741 cttggtaatc agtaacgctt gcccttcatt ttctgcggca gccttgaggt tgttggaggc
     1801 aacgacttta gccatggaac gcatcacctc ctcatcaaag gaaatgtcat tgatctggag
     1861 gtcgatcagg tgataacccc attcttccag catggcatcg acattgccct tcaccacttc
     1921 tacaatttca cgacgcaggg aaagcacctc actttgcttt ttcgtcgcca caaaagccct
     1981 gatattccct tctactactc ttgataaagt ctgcataaaa ctcctctcat cgataaattt
     2041 aaaagccacc gcctttacag tttcttcact gttgttgagt actgcataca atagcatgga
     2101 agtaaagtgc acattggcct gatcagaagt cgtagcgtgg aattccagct ctatggatct
     2161 gttctggata gaaatggtct tatacacctg ttccaaaaag ggtatcttaa aattcaatcc
     2221 gggcgtaaga atacgctgat atttaccaaa catagtgata acagctactg tgccctgacg
     2281 aactgtcacc aaaccagaga acacaaagag tgcagcgaaa actaaaagga taatcattgg
     2341 aatactcata tctaaatttt tttattaatt attataaatt gctaacaaag aatagtaata
     2401 tctaagttca tcatatcagc caatttcgat aaatcttata ttattcccga tttgcagcca
     2461 gatgcatggc cataaagcct tcttttttac gatcttctgc caggactctg tgggcatatt
     2521 gctgtaataa agctgatgct tcttgctggg ccaaaaggga tcgtatttct tcccggttag
     2581 tgaaggctgc caaatcctca tcgcccggga catcttctat acttgcaagg gatgcaagat
     2641 cattggcagt caggatatga ctggatttga aaaattccgg caatctttcg aatccaatcg
     2701 gagaagcttc ataaggctgg acaatagtgt ggactgcatc tccggatgct ctggagtaaa
     2761 aggcacggcc catcctcgcc atcaggtcta tcttgttcgg atctattcta tgattttcgt
     2821 ccataatcga tgagtcggcg tgaatcctga ccacttcaca aatcaccaga ttgcctgcac
     2881 cacctccttc tcccagaggt agaatgtcct tgaccttaca ttccaaatgt gccaagcatt
     2941 ccttgactct cggaggtttg accagatcag atggaatggg tgtcaatcct gccttttcaa
     3001 attcattgac ttctgaggga tagtcaacac tacagagtgt tacctgcctc actacatgat
     3061 aggaaactgc attgattaca cattcaccac tcgcctttac attggccagt gtatctttgg
     3121 ttgtattgtc cgatacccgg cgattggcac tgaaaatgac aatcggtgga ttgctgctga
     3181 aacaattgaa aaaactgaaa ggggccagat tggggattcc atgctcgtct aatgtggata
     3241 caaaggcaat tggcctgggg gctacaatgc ccagcaaaag ctggtgcaaa tctttggttg
     3301 ccatttctga cggttcgaaa tgcatatcta tggtttttta ttttgcgaaa taaccggttt
     3361 ttaactcaac aaaggtgttg tccacactcg ggagggttat gggatgcact tttcttacct
     3421 tgatccggat gcccaatatt ccaaagaatt cagactgaat ggcctcaatg accatctgag
     3481 ccccggtctc catcaggtcg cttcctttcg tgaaattctc ccgtgctatc ttttctaatt
     3541 gactgtaatc gaccgtgttt tctagttttt caatcagaaa tccatccgga acgtccatag
     3601 ccacctcaat atccatcaaa aaatcattgc ctaatacctt ctcctcagga tataggccga
     3661 caggagcgtg aagggataat ttttcaaagc cggaaataag gatcatcggt tgattttagc
     3721 ggaaaattat taatttatct gaaaattgat tcattcattg ataaaataaa ttataattgc
     3781 aggctaaaat tcgttgcatt tgcccgtact cagatcaata tataatacat tgttgtcgct
     3841 attggcgtat tttcggattt tttgctgtgt tttgtttttg gtcattgcag ttccctttct
     3901 gcatctcgaa acaatgatct ttcccaacaa ggaaaaaatc ggtttcaggt acagaaaatg
     3961 gtttacctcc ctatatcttt ttattctgaa tatcaaggtc aaaaagtccg gtgaaattgc
     4021 agcgcacaac gcgctcattg tatgtaacca caggatgatg atcgatccgg tgatcgtcct
     4081 ggattattgc cccggttaca tcgtaagcaa ggcggaagtt gcttcctatc ccatcctggg
     4141 acatggtgcc aggaatgccg gtgtcatctt tgtccggcgt gaccaaaagg aatccagaaa
     4201 tgccatcaag gagaaaattg gtgaattgct ttcagaaggt aagaatgtcg tcctttttcc
     4261 ggaaggcaca gtaaatacgg atagactgac agcaagattc tctgtagggt cgtttgaaga
     4321 agctgcaaaa tatggcaggc cggttattcc ggtcgctttg gattataaag atacctccgt
     4381 gtattgggga gccaacatct ctctgattca acactttttg caggtcttca gaaaacctcg
     4441 tcttgagacc cgtcttgtca tcggatctcc aattaagggt aatgatggct ttgaattgat
     4501 ggaaagtgca aagaatttta tcgacaatca gcttgaagtt attcgccggg actgggagac
     4561 aagccagatt taacttaggt ataatccgat gggaaagatt atttttatag tatgatgcat
     4621 gacaggaagt atacggcact catagttgtg gctttggtag gaatcctgct tttcctgcac
     4681 agaggtcatt ttaaccttga actaagaggt acacatgtct ggcgtcaatc cattacggcc
     4741 cttcatatcc gtaatttcgt cagatatgac aacaatatcc tgaatcccag agttgcctcg
     4801 ttcaacggtg ggcgcgacaa catcagccgt tatgaatttc cattgatgca atggaccatc
     4861 gctcaggtcg aaagagtgac aggtgagtct gttgctgttg tcaaattcta ccagtttctg
     4921 atagcgatat ccttcctgat cggattcatg tttttgttga aaagggcagg atttgaccgt
     4981 aggttctgtt ggatgggaat ctgggcagtg gctttctctc cggtcattta ttattattcc
     5041 gtatcaccta tcccggatat gctggccctg tcttttgcaa catgggcatt ataccatgca
     5101 attggttttt tcaggtcaga acaaaataag tattggccca tattcaatat cggactgctt
     5161 atttcccttg ccacgctggt taagctgcct tacattctct ttatggggcc cgctgtatac
     5221 ctgattattg ccaaataccg gatgcgttgg aaaagctatt ttgtcccctt ctttattctg
     5281 gcagcatgca tgattccggc gtttttgtgg tatgcctggg tcatccctca atggacagta
     5341 gggatcacca aaggtatgtt tgataatcag atatcgatgg atcatgcctt gcaaatcatt
     5401 tcttatcatc tgaaggtgat gtttccatac agattgctca atgttgtctt tgttcccttt
     5461 ttcctgctat ccgtaattcc gatgttccgg tctattcgga gggatgcggt gctccaaagc
     5521 atgttactgg gcttcctggc ctatctggct tattttttgt ttgagctcaa catgatcgat
     5581 gttgtccatg attattatat gatgccattt gttccattcc ttttttgggg tgtcatgaca
     5641 ggtattagat tttgctggca gctaagatat atggcagcaa aggttattgt tgggatagca
     5701 cttattgttg ccccttttac ctgtcttgag aatacgcaaa aagactggga gatcacccat
     5761 tttcatgata tttatgagaa acaggccgaa ttcataggtg cagccccgcc gaatgagaaa
     5821 gtcattatgc tgaaggattt ttcaggcttt atcctccctt acctgatcga tcaccacggt
     5881 catatcttca attttgatac ccttccccga agttgggtca aaaatatgat cagtgaatac
     5941 gatgtgcatt atttgtactc caactcccga acgtccgatt caatcaacag ggatttcatc
     6001 cgggatacgg tatttaaaca tggtgagttt aaagtgttca ggctgaaaga taagaccgca
     6061 gtggacacga tacctgctga atagcttttt atggcttata tggcctttgg caggagtaaa
     6121 taatggttgc agatgcgttt tcggcgatag ccccaatcta tatccgatca ttccctacct
     6181 attgatgact ttccgtattt tatgattagg gcaatcctga cagcactgat acctccttca
     6241 ttctatccaa agcggttttt gttttcgcag tgattcatcc ggctgataag ctgattatag
     6301 catatttttt ccaaattata attttatgac gaataattcg tctttaatta cgatatttac
     6361 gtcataaatt tatcaatagt gtttctcaat acccattctt ggtttagcct cagatatggc
     6421 acactgtcac cggagcggct ggctgatact gcccggtcgc tgggcatccg ggctatggca
     6481 ttgaccgata tcaacaatac atcctgtgcc tggcaatata tcgaggcctg tcgtcgcaac
     6541 gatgtccggc cgattcttgg tattgaattt cgccgggacg acggtcgtat gctatatgtt
     6601 ggcatagccc gtaacaatac cggttgggcc aatctttgcc gcatgctttc ggaagtgtcg
     6661 ctcgatg
//
LOCUS       DAOQMC010000125         6597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4884_length_6597_cov_4.808010, whole genome shotgun sequence.
ACCESSION   DAOQMC010000125 DAOQMC010000000
VERSION     DAOQMC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_6597_cov_4.808010"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            722..994
                     /locus_tag="PKV31_06165"
     CDS             722..994
                     /locus_tag="PKV31_06165"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HNB92474.1"
                     /translation="MLNKRMPHSCPACTAALEVTQLGCPACGTVISGNFPLPELLTLN
                     SDDQQFVLDFVRSSGSLKLMAQNLKLSYPTVRNMLDDIIEKLNQYP"
     gene            994..1572
                     /locus_tag="PKV31_06170"
     CDS             994..1572
                     /locus_tag="PKV31_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92475.1"
                     /translation="MKQILFNPFRTIAGAKALLIGWIIMLITACIAMYSQCHFDGAID
                     VHIGRNTTAIAYFLEPLVNWSVLTVIFYFTGLLLSTSHIRWIDFAGTVALARYPYLLA
                     AFIAFAMPTLDPESLTNFRDIDTSTLASLMITSILILLVSIWMIGLLYRAFTISGNLR
                     ERQAVWGFIIALILSELITGIIFHYLYKNLFL"
     gene            1569..2948
                     /locus_tag="PKV31_06175"
     CDS             1569..2948
                     /locus_tag="PKV31_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNB92476.1"
                     /translation="MKRYFFTASLFIFLLTNLHAQNITGDWNGTLNAMGFKLRLVFHI
                     TSQNDSLVAMMDSPDQGASGIPTSLVTFRHPDLHIEVGKGMIVYKGSMVTPDSIEGTF
                     SQSGRTFDLALKRGNVETRRPQEVKPPFPYKSEDITFANPTAGITLAGTLTMPEKGMK
                     FPAVVMISGSGPQDRNETIMGHKPFLVIADYMTKRGIAVLRFDDRGTASSQGKFDGAT
                     SADFATDVEAAVAYLRERKEIDSDKIGLIGHSEGAIIAPMVAAKDKGIAFIVMLAGTG
                     FDGADILVMQNEALGRASGLDEKDIEKATKINRILYNELVTGPGDSTLVEKTFVSLKP
                     LLSEKMSDEEILSFTDKSIKQLNTPWILYFLKNNPATFLEKVKCPVLAVGGSKDLQVP
                     ARTNLDAIEKALHKGGNKRVTIREFTGLNHLFQLADTGLPDEYGRIEETFSTEVLEFV
                     GKWMAGMIK"
     gene            3057..4340
                     /gene="purD"
                     /locus_tag="PKV31_06180"
     CDS             3057..4340
                     /gene="purD"
                     /locus_tag="PKV31_06180"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNB92477.1"
                     /translation="MVPYNILILGSGGREHAIAAHTVKSPLCNRLFIAPGNAGTAKVG
                     QNLPFAATDFIALKKNILEQDIRMVIIGPEDPLAHGIADKIHADSELQHVLVFGPGAE
                     GAQLEGSKAFAKRFMMKAGIPTASYREFSKDQLDETIEYLRSHPLPIVIKADGLAAGK
                     GVIIAPNVETGIDAVREIFGGQFGTSGDRIVIESFLDGIEFSVFIATNGEEYVLLPVA
                     KDYKKIFDEDKGPNTGGMGAVSPVPFVDNTMMIKVTEKIIRPTLAQLKQDNIPYTGFI
                     FFGLIAVGGDPYVIEYNVRMGDPETEVVFPRIKSDMIEIMEKLILHKPLPEMIIESDY
                     AVGVYLVSNGYPGDYEKGKAIDVDSLPEYCTLYHGGTKQDESGTLVSNGGRVIFSCAK
                     DSSLTSARRKALEGADSIVMDGKFYRRDIGLDLDH"
     gene            4601..6154
                     /locus_tag="PKV31_06185"
     CDS             4601..6154
                     /locus_tag="PKV31_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNB92478.1"
                     /translation="MTRYTSLFIFILLAFITVSGLSCKSEVKKAIEPPKPAELFNRYK
                     ESVVLIKTSFYARLVLDNGLTLYFSNPDNQEGDPFTEDENEARQNPLTIFGTGFFLTR
                     DGVIATNRHVAYPILEDLDVLGMLKQRYDALKSFIIHEIEINNDTLNMIDNALNQNPT
                     DSQIDILKEKAEEIVNRNIFYRIYLASNDFDPKQSSLEIIPVKVEIAYNNSFADENSG
                     YSACNILGKSEDKEIDLAILQLKDRHTPRKVTTLFDFENHDPNIENGTADSTDTYDLN
                     NSLEINKKLYMIGYNHGLTVATTSDGLKAQFTQGSVSQESDKYRVLYSIPALQGSSGS
                     PIIDRWGNLVAVNFAMVAGTQSFNYGIMAKHLKNLLHYYHNGNSTHSFDFGTTSLESQ
                     NNQNVAVSNDENSRPDFRTIIHNFLLAEDKRDFSAIYPFFSNNIRRYWDMTNPTETDL
                     RTRYGKLWRKSIHSQNNITKIEKINFYTYDVSTHFSFTDIDNNYKEVYATFRIVFDDT
                     GKITEIFGK"
     gene            complement(6222..>6597)
                     /locus_tag="PKV31_06190"
     CDS             complement(6222..>6597)
                     /locus_tag="PKV31_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92479.1"
                     /translation="GILLLTSSRKITLEHKRNVLRELWLGTETSTTYPPSIWYYLNYN
                     PVPGRISLRERLISNWKSFGQIRKTENNPDGDLDIYFGDGGKYTIDELENRANMYDQI
                     ESQIKLMKQDLRNLKESIISVK"
BASE COUNT         1999 a         1438 c         1334 g         1826 t
ORIGIN      
        1 cccaaaaatc gaagtaggta ggaaagtaat aattcaaaaa ccgccataca caaggttttg
       61 gagacaaatg aaaaatttaa ttcccgaata attacctgaa ttttggcaag gccaacccat
      121 aaggcaatac ctgcaataat tgtgactatt aatcatctac ctacttcaga ggcggcacca
      181 cccccattcc ccaaaaatcg aagtaggtag gaaagtaata attcaaaaac tgccatacat
      241 aaagatttgg ggacaaatga aaaatttaat tcccgaataa attacctgaa ttttggaaag
      301 gtcaacccat aaggcaatac ctgcaataat tgtgactatt aatcatctac ctacttcgga
      361 ggcggcaact cccatatcta tttcaaaaac ctactgaaat tctttatatg ccatcatccc
      421 accatcttaa tgatcattcc gccatatcat gacactgccg gagatgataa taaccgtaaa
      481 cccaatctca tgaagtattg tcacacccat caactccaag atgttcggca actatttagg
      541 ctcaaacaca tgattgaagt cgaagaatga tcaatccgtc tcctcctgta tctattcgga
      601 agccctcata ataatttagc agattcattt ataatagtat cttatgttga attaactaat
      661 tattatttat agtttaacta tattaaataa ttatttattt ttgtgaatat aaaaataaat
      721 aatgttaaac aaacgaatgc ctcattcctg tcctgcatgt actgcggcac tcgaagtaac
      781 acaattgggc tgtccggcct gtggtacggt aatctcaggt aactttccac tcccggaatt
      841 actcactttg aatagcgacg atcaacagtt tgtcctcgat tttgtccgct caagcggtag
      901 cctcaaactc atggcgcaaa acttaaaatt gagctatccg actgttcgca atatgctgga
      961 cgatatcatc gaaaaattaa atcagtatcc ataatgaagc agatcttgtt caatcctttc
     1021 cggacgattg ccggcgcaaa agcccttttg ataggatgga ttatcatgct gattaccgca
     1081 tgcattgcca tgtatagtca atgccatttt gacggagcca tcgatgtaca tataggccgg
     1141 aacacgactg caatagcata ttttctggaa ccattggtga attggtcagt attgaccgta
     1201 attttctact tcaccggtct gttgctttct acttcccaca ttcgatggat cgattttgca
     1261 ggtacagtgg ctttggcaag atatccgtat ttattggcgg cattcattgc ttttgctatg
     1321 ccgacactcg acccggaaag cctgacaaat ttccgggata tcgacacttc cactttggca
     1381 agtctgatga tcacatcaat cttaatattg ttggtttcga tctggatgat aggacttttg
     1441 tatcgcgcat tcactatctc tggaaatctg cgcgaaaggc aagctgtctg gggctttatt
     1501 attgctctga ttttatctga acttatcacc ggaatcattt tccactattt gtataaaaat
     1561 ttattcctat gaaaagatat tttttcactg catctttatt catcttcctt ttgactaatc
     1621 tccatgcaca gaacatcaca ggggattgga acggaaccct caacgctatg gggtttaaac
     1681 ttcgactggt attccacatc acttcacaaa acgatagcct ggtcgcaatg atggacagtc
     1741 cggaccaggg agcttcaggc attcctactt cactggtcac cttccgacat cctgacctgc
     1801 atatcgaagt aggcaagggt atgattgtat ataaaggaag tatggtcaca cccgatagca
     1861 ttgaggggac attctcacaa agcggcagga ccttcgatct ggctttaaag cgcggtaatg
     1921 ttgagactcg aagaccccag gaggtgaaac cgccatttcc ctataaaagc gaagacatta
     1981 ccttcgccaa tcccacagca ggtatcacat tggctggcac cctgaccatg ccggaaaaag
     2041 gaatgaaatt tccggctgta gttatgattt caggcagtgg gccccaagac aggaacgaaa
     2101 ccatcatggg acacaaaccg tttttagtca ttgccgatta tatgacaaag cgaggcatcg
     2161 cggtattacg gttcgacgat aggggcactg catcctcgca gggtaaattc gacggagcta
     2221 catcagcgga ttttgccact gatgtagagg ccgcagtggc atacctgcgc gaaagaaaag
     2281 aaattgactc agacaaaata ggtttaatcg gtcacagtga aggtgccatc atcgccccca
     2341 tggtggcagc aaaggataaa gggattgcat ttatagtaat gctcgccggc actggttttg
     2401 atggggctga tatcttagtg atgcaaaatg aagcattagg cagggcatca ggactggatg
     2461 aaaaggatat agaaaaagcc accaagatta atcgcattct gtacaatgaa ctcgtcaccg
     2521 gaccaggtga ttccacactt gtagaaaaaa cttttgtctc tcttaagccc cttctaagtg
     2581 aaaaaatgag cgatgaagaa atcctctcat ttacagacaa atcaatcaaa caactgaaca
     2641 caccttggat tctatacttt ctgaaaaaca accctgccac ttttttggaa aaagttaaat
     2701 gcccggtatt agcagtcggt ggatcgaaag acttgcaggt gcctgcccga acaaatctgg
     2761 atgctataga aaaagccttg cacaagggag ggaataagcg tgtgaccatc cgcgaattta
     2821 cgggattaaa ccatttgttc caactggcag acacgggtct cccggatgaa tatggtagga
     2881 tagaagagac gttttcaact gaagttctgg aatttgtcgg aaaatggatg gcagggatga
     2941 taaaataaac catgttaacg gacttactgc aaaaacaaat aattttcatt ttccctttat
     3001 ccattatagt tatcaacctt tcctattttt gcatcttgac aaacctactt taatcaatgg
     3061 tcccatataa tattctgatc ctgggaagtg gtggaagaga gcacgccata gccgcgcaca
     3121 cagtgaaaag ccctttatgc aatcgtctct tcatagcgcc cggaaatgcc ggaacagcta
     3181 aagtgggcca aaatctgccc tttgcagcaa ctgatttcat cgcgttgaag aaaaatattc
     3241 tcgaacaaga catccgaatg gttatcatcg ggcctgaaga tccgcttgca catggcattg
     3301 cagacaagat tcacgcagat tcagaattgc aacatgtact tgtcttcgga ccgggcgcag
     3361 aaggcgcaca actggaaggt tccaaggctt ttgccaagcg ctttatgatg aaggccggca
     3421 ttcctacggc atcgtatcgg gagttttcca aggaccaatt ggacgaaacc attgaatatc
     3481 ttcgctcgca tccattaccc atcgttatca aggcagatgg cctggctgca ggtaaaggcg
     3541 tcatcatagc accgaatgtt gagaccggca tcgatgccgt acgagagatt ttcggtggac
     3601 agtttggaac ctccggagat aggatcgtca tcgaatcctt tctagacggc attgaatttt
     3661 cagtatttat agccaccaat ggtgaagaat atgtcctgct tccggtagca aaagactaca
     3721 aaaaaatctt tgatgaagac aaaggtccca atacgggagg catgggagcc gtcagcccgg
     3781 ttccatttgt tgacaatacg atgatgatta aggtcactga aaagattatc cggcctaccc
     3841 ttgcccagct gaagcaggat aacattccct ataccggctt catcttcttt ggacttatcg
     3901 ctgtcggtgg agatccttac gtcatcgaat ataacgtgag gatgggggat cctgagaccg
     3961 aagtcgtctt tcctcgcatc aaaagtgata tgatagagat catggaaaaa cttatcctac
     4021 ataagccctt gcccgaaatg ataatagaat cggactacgc cgtaggtgta tatttagtgt
     4081 caaacggtta tcccggagat tatgagaaag gtaaggccat cgatgttgat tctcttcccg
     4141 aatactgcac cctgtaccat ggaggtacta agcaggatga atcgggtaca ctcgtgtcga
     4201 acggtggccg ggtgattttc tcgtgtgcaa aagattccag tctgactagt gcacgcagga
     4261 aagctttgga aggagcagat tctatcgtta tggatggaaa attctacaga agagacatcg
     4321 ggcttgatct ggaccattga tagtcaataa atcaccttta tggaaccatc ggtaaatttg
     4381 cgatcagggt aaatttcgat tagtagttta cggtacaaat gaccgaacca ataactccac
     4441 ccggatcatg taggtctaat aattcttact gttttgagtc aaaagtatta gtctattcgc
     4501 tccggattga atttttttac cggattttag gtttattaat tcaggtcttt ggatcaatgc
     4561 ttttgcacaa gttattatcc caaaatcagt cgaacatgat atgacacgtt acacttcgct
     4621 atttatattt attttacttg ctttcatcac cgtatccgga ttatcctgta aatcagaggt
     4681 aaagaaagcc atcgagccgc ctaaaccggc tgaattattc aaccggtata aggagagtgt
     4741 tgtgctgata aaaacaagtt tttatgcaag gttggtactt gacaatggcc tcaccctata
     4801 tttctcaaat ccggacaatc aagaagggga cccatttact gaagatgaaa atgaggcaag
     4861 acaaaatccg ttgaccattt ttggcacagg cttttttctt acaagggatg gtgtgatagc
     4921 caccaatcgc catgtggcat atcccattct ggaagacctt gacgtgctgg gcatgctcaa
     4981 gcaacgatac gatgcgctca aatcatttat cattcacgaa atcgaaatca ataatgacac
     5041 cctgaacatg attgacaatg ccctcaatca aaatcctacc gattcacaaa tagacatttt
     5101 gaaagagaaa gccgaagaaa ttgtaaatcg aaatatattt tacaggattt atctggcttc
     5161 taatgatttt gacccaaaac aatccagcct tgaaattatc ccggtaaagg ttgaaatcgc
     5221 ctataacaac agttttgcag atgaaaattc gggatattcg gcctgtaata ttctgggcaa
     5281 atcggaagac aaagaaatcg acctcgccat ccttcaactc aaagacaggc ataccccccg
     5341 gaaagtcact acgctatttg attttgaaaa tcacgatcct aatatcgaaa atggaacggc
     5401 ggattctact gatacttatg acctgaacaa tagtctggaa attaataaaa agctatacat
     5461 gattggctac aatcacggcc ttaccgttgc cactacctcc gatggcctca aagcacagtt
     5521 tactcaaggc tcggtaagtc aagaatctga caaatatcgt gtcctttatt ccattcctgc
     5581 cttgcagggc tcaagcggaa gcccgattat cgatcgatgg ggcaatcttg ttgctgttaa
     5641 cttcgcaatg gttgcgggca cacaaagttt caactatggc attatggcaa agcacctaaa
     5701 aaatcttttg cattattatc acaacgggaa ctcaacccat tctttcgatt ttggcactac
     5761 atccttggaa agtcagaata atcaaaatgt agcggtatct aatgatgaaa attcaaggcc
     5821 tgacttccgg accatcatcc acaacttcct tctagctgaa gataaaagag atttttccgc
     5881 catatatcct ttcttttcaa ataatatcag acgttattgg gacatgacca atccgacaga
     5941 gactgacttg agaaccagat acggaaagtt atggagaaaa tctatccatt ctcaaaacaa
     6001 catcacaaaa attgaaaaga tcaattttta cacctacgat gtcagcacac atttttcatt
     6061 tacagatatt gacaacaatt acaaggaagt atatgccacc tttcgcatcg tttttgatga
     6121 taccggaaaa attaccgaga tatttggcaa ataaggcgtg actgttatat agggcgttga
     6181 agggtttata tcattacaaa tagttttttc acacacctgt tctacttcac agaaatgata
     6241 ctttccttca gatttcggag gtcctgtttc attaatttaa tttgcgattc gatctgatcg
     6301 tacatgttgg ctctgttctc gagttcgtca attgtgtatt ttccaccgtc accgaaataa
     6361 atatccagat ccccatccgg attattttcc gttttccgaa tctgaccaaa acttttccag
     6421 ttactgatca atcgctccct taaagagatc ctgccgggta ccggattgta attgagataa
     6481 taccaaatcg agggcggata agtagtcgag gtttcagtac ccagccataa ctcacgtaag
     6541 acatttcttt tatgttccag agtaatcttg cgactgcttg tcaaaagtag tattccg
//
LOCUS       DAOQMC010000126         6575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4915_length_6575_cov_5.069325, whole genome shotgun sequence.
ACCESSION   DAOQMC010000126 DAOQMC010000000
VERSION     DAOQMC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4915_length_6575_cov_5.069325"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..943)
                     /locus_tag="PKV31_06195"
     CDS             complement(386..943)
                     /locus_tag="PKV31_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNB92480.1"
                     /translation="MIIDNTWTLFLDRDGVINRRILGGYVTKIEEFEPLPGIFEAIRT
                     FNKIFARTCIVTNQSGVGKGLMSQEDLNNIHSHFMDLLNEKNAIIDGIYCCTSLETDI
                     DNCRKPLPDMAIRAKQDFREIDFSRSIMVGDTGSDMEFGYNIGALNVLILDGSEHQKK
                     IDEELVNLRFPSLLEFSQFLVASYN"
     gene            complement(956..1543)
                     /locus_tag="PKV31_06200"
     CDS             complement(956..1543)
                     /locus_tag="PKV31_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNB92481.1"
                     /translation="MNYSRDISKILSEHENVVKSVINDSTLLAAIEEFTRLVVQCYTH
                     GGKILLCGNGGSASDAQHIAAEWQGRYLLERKPLHAEALHANTSYLTAVANDYGFDKV
                     FSRAVEAQGKGGDILVGLTTSGNSPNIVNALKTGKSLAMTCIGMTGNKTDPAIEPYCD
                     LMIKVPSDVTARIQEIHILIGHIVCELSEKSLFKS"
     gene            complement(1540..2493)
                     /locus_tag="PKV31_06205"
     CDS             complement(1540..2493)
                     /locus_tag="PKV31_06205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB92482.1"
                     /translation="MTNERIQISVVVPLYNEDESLPELAAWIHRVMKANGFTYEIIMV
                     DDGSKDQSWEVIERLSKENNSVKGIKFKRNYGKSAALHEGFCASTGDVVITMDADLQD
                     SPDEIPELYKMIKEKGYDLVSGWKKKRYDPLSKTIPTKLFNAAARAMSGIRLHDFNCG
                     LKAYKGKVVHSIEVYGEMHRYIPVIAKGAGFGKIGEKVVQHRERKYGVTKFGLDRFIN
                     GFLDLLTIFFVGRFGKKPMHFFGTLGIIAFFIGFIILIELSIEKIFFKVGGIADRPLF
                     FLGMLATIIGSQLFLTGFLAELITRNSPSRNNYLIEEKIHL"
     gene            complement(2536..3084)
                     /locus_tag="PKV31_06210"
     CDS             complement(2536..3084)
                     /locus_tag="PKV31_06210"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNB92483.1"
                     /translation="MSNNVEMEQRPSFTQKSVLNTAMLLALVSIITALIPSVLDLQGD
                     TKSGVFITLVSYLITIFILVNALKKYRNEESGGYMSFSNGFLFVLYTSIIVGIIMAIF
                     TFIQLTFIDADMLDEAFNKQMAEMEKNGMSEEQIEMANSITAVFRSPAVLSTAAFLGQ
                     LVFGAIIGAILGAIFQKNKPVY"
     gene            complement(3097..4299)
                     /locus_tag="PKV31_06215"
     CDS             complement(3097..4299)
                     /locus_tag="PKV31_06215"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB92484.1"
                     /translation="MSSRITMDHVLKAYDPDSKLTNLTIENGQIISERSSDSGEFNAV
                     LFSNLIDFGTKIAEPGYEHRETLEQTCNAAHYGGYKAIITLPVTDPVVDNQSGIEFLT
                     RRSEKYGGIKIFPMGAISEGCKGGDLAGLMEMNHAGAIAFSDGHYPIRNGGVLMRAMD
                     YVRGIDKIIVDTPYDADLFPHGLVEEGMVSTIMGVPGIPAPAEEIVVNRDIRLAEYTG
                     ASLHLFCISTEGSIEQIRKAKASGIKVTASVSANNLVFDSEEMVTYNSNLKLMPPLRN
                     REVSRLLWEAVKDGTIDIITSQHMPYEVEKKDLEFQYASFGALGLQTAIPVLLSKFGV
                     EAAPYIQKAMSDNIIKIFKLEKGFEDQKLTLFEKCNPYTFREDVIISHCNNSPFIGRE
                     LTFQNMMI"
     gene            complement(4653..5435)
                     /locus_tag="PKV31_06220"
     CDS             complement(4653..5435)
                     /locus_tag="PKV31_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92485.1"
                     /translation="MRSLLTFFFIFSMNFMFGQVCIPDTTVIPTRSFIYPIPRQDTIP
                     GSGIPNKACLNTYYEQVFHVKIPLQVEFLGFNVHVQNVKLNSITGLPAGLSYACEPTG
                     CSMPGNTIGCMKISGVVDPSNSVKTYPITLNFTFTTAELGPLDLSFPNDAIAPGHYDI
                     QVLPEGSSECIASASKLNIVQKAFHNISTGNLEFELESQDQINATINILNMDGRMMKS
                     AGVKIENGKNHFYISSGQMNTGIYIYQIKSGNRIQSGKIFIY"
     gene            5500..>6575
                     /locus_tag="PKV31_06225"
     CDS             5500..>6575
                     /locus_tag="PKV31_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92486.1"
                     /translation="MKFQNFTLALFLILNFSIFGQNRKLEIKVVDSHDGTPLEFVAIY
                     AEDKGELTNSQGVATFILDNSPVSVRAGLVGYETWRGEVSLDTIKGYFTIRLQPSVSM
                     LNEVSVVSSRYERKLITEVASVDIIKPGLLKSNNLSSIEGLLDRIPGIQMVDGQANIR
                     GGSGYSYGAGSRVLITIDDVPAMQPDAGFPNWRDIPVENMEQVEILKGAGSVLYGSTA
                     MSGVINFRTAWAGSKPETNISTQFTGYLSPRDKEKKWWSGLPYESNTYVIHRNNFGKL
                     GVVLGAAYYHSDSYNYGNFEHQGRIFANLRYKINNNLIVQVNTLANKGESDFFVYWQD
                     GDKNAYRGDTSSYAPGEKLRYLIDP"
BASE COUNT         1939 a         1302 c         1277 g         2057 t
ORIGIN      
        1 tgacacaatg actaattatt tttggcttgg ttttagtgaa atgattaata ttaatttttc
       61 aatttgcttt ataacatagg gatgcgaggt attagacatc tacctaattc taatttttca
      121 ctttcaaagt gattttacat tccttccaaa tcgaagtagg tagatgatta atactgtgca
      181 atccaggcat tataaatcgc ttgcagtaat ttgaaaaaaa tcacaaggtt ttctacctta
      241 ttattgagct aataatccta aaatgccttt ggaaaaagag tttgggtgta ttaagcaaat
      301 acctaattct acataatgtt ataatacaca gataaacaat agtatataca tataataaat
      361 gcaatattta tttatctccc accccctaat tgtaggaagc caccaaaaac tgtgaaaact
      421 caagtaagga gggaaatcga aggttaacta gttcttcatc tattttcttc tgatgctcgg
      481 aaccatcgag tatcaaaaca ttgagggccc cgatattata tccaaactcc atgtcagacc
      541 ctgtatcacc taccatgatg gacctggaaa agtctatttc ccggaaatcc tgttttgccc
      601 tgattgccat atctggaagc ggcttgcggc agttgtcgat gtctgtttct aaggatgtac
      661 aacaatagat accatcgatg atggcattct tttcattgag taagtccata aaatgagaat
      721 ggatgttatt aagatcttcc tggctcatca atcctttacc tacccctgat tgattggtga
      781 caatacatgt acgagcgaat atcttattga atgttctaat ggcttcaaat attcccggca
      841 aaggctcgaa ttcttcaatt tttgttacat agcctccaag gatacggcgg ttgatgacac
      901 cgtcccggtc aagaaacaag gtccatgtat tgtcgatgat caaaatagtt ggaatttaag
      961 acttgaagag acttttttca gacaattcac acacaatatg accgatgagg atatgtattt
     1021 cctgaatacg tgcagtcaca tctgaaggca ctttaatcat caggtcgcaa tatggctcaa
     1081 tcgcgggatc tgttttattt cctgtcatac ctatacaggt catagccagt gatttcccgg
     1141 ttttcaaagc attgacaata ttgggagagt tccctgaggt agtcagaccc accaaaatgt
     1201 ctccgccttt gccttgagct tctacggccc ttgaaaacac tttgtcaaat ccataatcat
     1261 tagccactgc ggttagatag gatgtattgg cgtgtagtgc ttccgcatgt agtggcttcc
     1321 tttccaacag gtaccttcct tgccattctg cagcgatatg ttgagcgtca gaagcgcttc
     1381 ctccattgcc acacaataat atcttgcctc catgagtata gcattgtact actaacctcg
     1441 taaattcttc tatggcagca agcaaggtgc tgtcattgat tactgacttt acgacatttt
     1501 cgtgttctga aaggatttta gatatgtccc gggaatagtt caaagatgga ttttttcttc
     1561 aattagataa ttattacgcg aaggactgtt tcttgttatt aattctgcta aaaagccggt
     1621 taggaaaagc tgagatccga tgatggtggc cagcataccc aggaaaaaca gtggtctgtc
     1681 tgcaatccca cctaccttga agaatatctt ttcaatactc aattctatga ggataataaa
     1741 gccgataaag aatgctatga tgcctaaagt gccaaaaaag tgcattggtt tcttgccaaa
     1801 gcgacccaca aagaatatcg ttaaaagatc cagaaatcca ttgataaatc gatctaaacc
     1861 gaatttagtg acgccatatt ttctttctcg atgctggacg accttttcgc ctatctttcc
     1921 aaatccggct ccttttgcaa ttaccggaat ataacggtgc atctcaccat atacctctat
     1981 agaatgtacc accttgccct tataagcttt aagtccgcag ttgaaatcat gcagtcgaat
     2041 tccgctcatg gcacgagctg ctgcattgaa taacttggtg gggattgtct tgcttaatgg
     2101 atcgtaacgt tttttcttcc agccggaaac caggtcatac cctttttcct taatcatttt
     2161 gtacaattca ggtatttcgt ccggactatc ttgcaaatct gcatccatgg tgattaccac
     2221 atctccggtg gaggcacaaa agccttcatg taaagcggcg gatttgccat aatttctttt
     2281 gaattttatc cctttgacgc tgttgttttc tttactgagt ctttcgatta cttcccatga
     2341 ttgatccttg cttccatcat ccaccataat gatttcgtag gtgaacccat tggctttcat
     2401 gacacggtga atccaggcag cgagctccgg taaggattca tcctcattgt ataaaggaac
     2461 aacaaccgat atttgtattc tttcgttagt catgcaacca ttttaatgct tcgtcggaca
     2521 atagtatatg aataatcaat aaaccggctt gtttttttga aatattgcac ccaggatggc
     2581 cccaataata gctccgaaaa ccaattgacc cagaaaggct gcagttgaaa gtacagccgg
     2641 tgacctaaag accgctgtaa tgctattggc catttcaatc tgctcttcag acatgccatt
     2701 tttttccatt tcagccattt gcttattgaa tgcttcatcc aacatatctg catcaataaa
     2761 tgtcaattga atgaaggtaa atattgccat gataatgcct acaattatac tggtatatag
     2821 aacaaacagg aagccattac tgaaactcat ataacccccg gactcttcat tcctgtattt
     2881 tttcagggcg tttacaagta taaagatggt tatcaaatag gagaccaaag taataaaaac
     2941 tccggacttc gtatccccct gtaggtccaa aacgctggga atcaatgcgg ttatgatgga
     3001 taccagtgcc aatagcattg cagtatttaa cacagatttt tgggtaaatg atggtctttg
     3061 ttccatttct acattattac tcatcttaat atttttttat atcatcatgt tttggaatgt
     3121 caattctctc ccaataaaag gagaattgtt gcaatgcgat atgattacat cttccctgaa
     3181 agtatatgga ttacattttt caaaaagggt caacttttga tcttcaaatc ctttttcaag
     3241 tttaaaaatt tttataatat tatcactcat tgccttttgg atataaggag cagcttcgac
     3301 cccaaatttg gatagcaaaa caggaatagc agtctgaagt ccaagagcac cgaaagatgc
     3361 gtactggaat tccagatctt ttttctcaac ttcatacggc atatgttggc tggtgatgat
     3421 atctatggta ccatctttca ccgcctccca taataacctg cttacttccc tgtttctcaa
     3481 tggtggcata agtttcagat ttgaattgta tgtcaccatc tcttcggaat cgaacacaag
     3541 attgttggct gatacagatg ctgtaacctt gattccggaa gcctttgcct ttcttatttg
     3601 ttcaatgctc ccttctgtac ttatacagaa tagatgtaga gaagcacctg tgtattcagc
     3661 caatctgata tctcggttga ctacaatctc ttcagcaggt gcaggaatac cgggcactcc
     3721 cataattgtt gaaaccatac cttcttctac gaggccatgg gggaacaggt ccgcatcata
     3781 gggagtatca acgatgattt tgtcgatgcc acggacatag tccatcgcac gcataagcac
     3841 tccaccattg cgaattggat aatgaccatc gctaaatgca atcgcccctg catggttcat
     3901 ttccataagc cctgcaagat cacctccttt acaaccttca ctgatggcgc ccattggaaa
     3961 aatcttaatt ccaccatatt tttcactccg gcgagtcaga aactcaattc ctgattggtt
     4021 gtccacgaca ggatcagtaa cgggtagggt gattattgct ttatagccac cataatgagc
     4081 cgcattacat gtttgctcca aagtctcccg gtgctcatac cccggttcgg caatctttgt
     4141 cccaaagtca attaagttgg agaataaaac agcattaaat tcaccggaat ccgaagaacg
     4201 ctctgatatt atttgcccgt tttcaatagt caggtttgta agcttactgt cagggtcgta
     4261 agccttcaga acatgatcca tcgtgatacg tgatgacatt tgataatcaa ttttaacttt
     4321 tgccggtcac aaatatccga taatttttct ttatagaaat tacaaagcat gctttattgg
     4381 acaaatatgg gtaaaatctc attggaagtg cgattcttaa atataaaaga aatcaaattc
     4441 atagaattgg acatgcggaa ttacattgct gcaagtgatt tttgaatttt aaagagagat
     4501 tcgaaataag ttgaggagtt gaagctattt gtaatattaa aatatataat atgtttatgt
     4561 ctttgttaat aaaaaaattg cgcaaagaat ccgggtttta aaaagcttcc ttgcgcaatt
     4621 ttaatatatg tcaaaattga tgaaagcaat atttaataga taaaaatctt tccggattgt
     4681 attctgttac ctgattttat ttgataaata tagattcctg tattcatttg tccggaagaa
     4741 atgtagaaat gattctttcc attttcaatc ttaacaccgg cacttttcat cattcttcca
     4801 tccatattta aaatattgat ggtagcatta atctgatcct gactttccaa ttcaaactca
     4861 agatttccgg tagaaatgtt atggaaagct ttctgaacaa tgtttagctt tgatgctgaa
     4921 gcaatacact cagaacttcc ttcaggtaaa acctgaatat catagtggcc gggtgcgatg
     4981 gcatcattgg ggaagctcaa atccagaggt ccgagttcag ctgtcgtgaa ggtaaaatta
     5041 agtgttatcg gataagtttt gaccgaatta cttgggtcga ccactccact aattttcata
     5101 catccgatgg tatttccggg catggagcat ccggtaggtt cgcaagcata gcttaaaccg
     5161 gccggtagac ctgtgattga atttaatttg acattctgca catgcacatt gaacccaaga
     5221 aattcaactt gtaatgggat tttgacatga aatacctgct cataataggt attgagacaa
     5281 gctttatttg gaatgccgga gccgggaatg gtatcttgcc gtggaatcgg ataaatgaat
     5341 gatctcgtcg gtattacagt tgtatctggg atacaaacct gaccaaacat aaaattcatg
     5401 gaaaagataa aaaaaaatgt aagaagtgaa cgcatgttac agtaaatttg attacaattg
     5461 ctaaattaca actaattttc aaatttatat ttgaattata tgaaatttca aaattttact
     5521 ttggctcttt ttttgatttt aaacttttca atttttgggc aaaaccggaa actggagatt
     5581 aaagtagtcg acagccatga tggtacaccg cttgagtttg ttgctatata tgcagaagat
     5641 aagggcgaat tgaccaatag tcaaggcgta gctacattta tacttgataa tagtccggtt
     5701 tcggttcggg ctggcttggt gggatatgaa acctggcgcg gagaagtgtc acttgacacc
     5761 atcaagggat attttaccat cagattgcaa ccatcggtga gcatgctcaa tgaagtctcg
     5821 gttgtaagta gtcgctatga aaggaaacta attacggagg tcgcttcggt tgatatcatc
     5881 aagcccggac tgttgaagtc aaataatcta tcatccattg aagggttgtt ggatagaatt
     5941 ccgggaatac agatggtgga cggccaggca aatatccgcg gaggttcggg ttattcttat
     6001 ggcgccggta gcagggtact gattaccatt gatgatgtac ccgccatgca accggatgcc
     6061 ggttttccca attggcgtga tataccggtt gaaaatatgg aacaagtgga aatactcaaa
     6121 ggtgccggtt cagtacttta tggatctaca gcgatgagtg gtgttatcaa ttttcggaca
     6181 gcgtgggcgg gaagtaagcc tgaaactaat atctccaccc aatttacagg ctatctgtcg
     6241 cctcgtgata aggagaagaa atggtggagt gggcttccat atgaaagcaa tacctatgtg
     6301 attcatagaa ataactttgg gaaattgggt gttgtcttgg gggcggcata ttatcattct
     6361 gattcctata attatggcaa ttttgagcac cagggacgaa ttttcgccaa tttaagatat
     6421 aaaattaata ataaccttat cgttcaagtc aatacacttg ccaataaagg ggagtctgat
     6481 ttttttgtgt attggcagga cggtgataaa aatgcttatc gcggagatac gtcatcctat
     6541 gcccccggag agaaacttcg ttatcttatt gatcc
//
LOCUS       DAOQMC010000127         6564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4928_length_6564_cov_5.272853, whole genome shotgun sequence.
ACCESSION   DAOQMC010000127 DAOQMC010000000
VERSION     DAOQMC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4928_length_6564_cov_5.272853"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKV31_06230"
     CDS             complement(<1..573)
                     /locus_tag="PKV31_06230"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNB92487.1"
                     /translation="MNIRYNHSSLIIVVAWILSVATTFSCLGQAPSITIIASPGNSLC
                     VGDNLTMASTISDGGAFTFSYQWYKNNVEIPGATNDSYSIPNIQLIDAGTYKVVATYD
                     DMGNQEIDDQIIISVNPKPAITAIQKEDPTTCNGQDGSFTLVGLAGTGYQLFYTLNGT
                     EVGPINLPVNGEYKVSNLAAGNYTNIYVIKN"
     gene            complement(586..1812)
                     /locus_tag="PKV31_06235"
     CDS             complement(586..1812)
                     /locus_tag="PKV31_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92488.1"
                     /translation="MKIKLSFYFSLCCILIACSSEKSNNAIKDTMTHFSINGKDVTAY
                     RMTNKDQFDASNKSNYASVGANEDISLTVDLPKDVDMDVAWYINGSEMKGSSVKTKFP
                     IPETYEVVLQIPGFEEVVKYIIVEEQSTPVDLAIQDTVTQEMPTTENLPETAPKEVEV
                     KKENKPLPIQNREVQSQKKQPSVVKNEKIETKKVQKRTTPSSEVNTQPEIVDGPPPPP
                     KRVVTESSPSKSPPVKSEPTTGFNNKKTEGAPKPGSTTDVSKKKTEESSKSGTTIDLN
                     NKKTEGAPKTEPCKTATYSSTASVSLKPKQDIELSSFYVYAAQSGNVDIILMENNKQV
                     DKLVGRHVNAGMTQLRVSTLNTTLKGGKVYVLKITGHGGITFANLAPCGVGGGGSILN
                     PSYPGGVSIFNIHFNY"
     gene            complement(2909..6436)
                     /locus_tag="PKV31_06240"
     CDS             complement(2909..6436)
                     /locus_tag="PKV31_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92489.1"
                     /translation="MNTSYSPAKWPRFTLAGFAFFLINTFVVPIAANAQCGYIEGLGC
                     PGTNYSNYGSASNHDAASIEYDNFISAFHTSILRDPEGGFRIWGENTAPNGKSVLWIP
                     TPIDAFHFPLIKARPLKAAMGSKAHRNVQSFVLTADDKLWAWGKRGAVLHAAVTKSDS
                     MQSIILPRGIHAMDVKTLFATTEALAMLTCNGDVYVMAQHESMRGQSTLTSFNSWTRV
                     RTGAGAKAKSLDNIIVLRGSASGFIALDSEGQLWTWGRSTYLGDNNPAQSRAFAEKMI
                     LPSNSGSIKMVGATGSLTAGEITYYVLYGDGRLFALGNGMGGQLGNWSIGNSNTWIQP
                     RYAVSEESVMNNIQWFSPNEHDYKYPFVNVITRDSTLYNWGLESGYALGRRVHGLTNS
                     AKATNPGIPPSLQGMKIVAVESGCHTTVVDAQCMGTYGYVGHQVEGSLGDGTDEGEVH
                     QDFVFGNSYLQTCGALTAPVISVDDDYMKGTHGKICTNQTVHINAAPGNGVLSINTEP
                     VGIAQLNGNNLEIQGVGDISLNYHVQDFHTCESGLSVTIKLETETCTINAVRGTVWID
                     DDLDAIKDQAESQSIGSYGSNIGLWANLIEASGVVYNSVPVNPNGTFEIITAQKGTYS
                     VQVTKSKVIVGSIVRSSDKILPEGWKYTGNNHSNNSICKVPDCTDPSVFSNLVLGNND
                     INGINFGIIGAYSLSGRVFHDADGLNKNGVNGNPVYPSSSYVQQGLPPLFVCAVDKKG
                     KVANYTSVRNDGTYQINIPIKQDITLLLTTTLPTIGGPSPLPSLPSGWTFVGETFGTN
                     NTLGSGNNDGSGSGANSPMTRYDGKVSINIQGNESSVGEVDFGIQQLPGADPKSYSVN
                     ISDFSSIPPNGYPIITNFKAITMSSPKLKESGNNSNGSLSGSDPEDCAADGTCNGNQG
                     GIRTTFTIGTIHSNTRLYYDFGGVVGIREILANSVISNFNADKMVIYGQDGEGNTHNP
                     FGFEYTITDKAGTNGVFVTYGIQLNAPLPLNLLSFDVVRKEAAAYLSWTSLDERQFRG
                     FEVLRSTDGRIWTTVTFVPSNGISGARQDYFFIDHKPAEGLNLYKLKMIDQDEKYQYS
                     DIRSVTFNIKNHASVYPNPTDGHLNITGLSGNETIRVFDLTGKVLMKVDAKDQQGGMS
                     LTGYSAGTYFVHITYVDGKTEIIKVEKIK"
BASE COUNT         1836 a         1397 c         1327 g         2004 t
ORIGIN      
        1 attcttgatg acataaatat ttgtataatt tcctgctgca agattgctta ctttatattc
       61 cccattgaca ggcaggttga ttggcccaac ctctgtacca ttcagggtat aaaatagctg
      121 atatcctgtg cctgctaaac caacaagcgt aaaggacccg tcctgtccat tacaggttgt
      181 agggtcttct ttttgtatgg cagtaatcgc tggtttggga ttgacggaaa ttattatctg
      241 atcatctatt tcctgattgc ccatatcatc ataagttgct actaccttat aggtaccggc
      301 atctatcagt tgaatatttg gaatcgaata actgtcattt gttgcaccgg gtatttcaac
      361 attgtttttg taccattgat aactaaaagt aaaggcaccg ccatcgctta ttgtacttgc
      421 catggtcaga ttgtctccta cacaaagtga attgccaggc gaagctataa tggtgatgga
      481 tggagcttgc cccaaacaag aaaatgtagt agctactgaa agtatccacg caactactat
      541 gattagcgaa gaatgattat atctgatatt cataataaaa aacagttaat aattgaagtg
      601 aatattaaag atggaaacac cacccggata ggaggggttc aatattgaac ctccaccacc
      661 tactccacaa ggggcgagat tagcaaaggt aatgcctcca tgtcccgtaa ttttcagtac
      721 atacactttc cctcccttta atgttgtgtt cagggttgaa actctaagtt gtgtcattcc
      781 ggcattgaca tgtcgcccaa ccagtttgtc aacctgcttg ttgttttcca tcaagataat
      841 atctacatta cccgattggg cggcataaac ataaaaactt gatagttcaa tgtcctgttt
      901 gggtttcagg gacacagaag ctgtcgaaga ataggttgct gttttacagg gttctgtttt
      961 gggagccccc tctgtctttt tgttatttaa gtcaattgtt gtccctgatt tggaagactc
     1021 ttccgtcttt ttcttactga cgtcagtcgt tgaccctggc ttgggtgccc cctctgtctt
     1081 tttattattg aagccagttg ttggctctga cttgaccgga ggagacttgc ttggactgga
     1141 ttcggtaact acccgcttag gaggaggagg tgggccatct acaatttccg gttgagtatt
     1201 cacttcggat gaaggtgtag ttcttttttg tacctttttt gtttcaattt tttcattctt
     1261 aaccaccgac ggctgctttt tttgtgactg cacctctcta ttttggatag gtagcggttt
     1321 gttctccttt ttcacctcga cttctttagg ggcggtttcc ggcaaatttt cagtggttgg
     1381 catttcctga gtcacagtgt cctggatcgc caaatcgaca ggtgtacttt gttcttcaac
     1441 aataatatac ttcacaacct cttcaaatcc aggtatttga aggacaactt cataggtctc
     1501 cggtatcgga aatttggtct tgacagaact tcctttcatt tctgaaccat tgatatacca
     1561 tgccacatcc atgtcgacgt ctttgggtag atccacagtc aaagaaatat cttcattggc
     1621 acctacagaa gcataattgg atttattgga tgcatcaaac tgatccttat tggtcattct
     1681 ataagcggta acatcctttc cattgatgga gaaatgtgtc attgtatctt taatagcatt
     1741 attggatttt tcgcttgagc atgcaatcaa aatgcaacat aaagagaaat agaacgataa
     1801 ttttattttc atataaaaat atttatatca aagatttcgg tccttcaagt attacggaca
     1861 tcttccgggg tttttactaa aatgctatgg attgcttata actaaatatc tgaagtgtca
     1921 ctcaatgtga tattaaggca caagtattat agcgctcaaa tataacaaaa atcgaaaaaa
     1981 tttatacaat tattatcctt taattgtatt ggtaaaatga aactaattat attcggaggt
     2041 ttctgaataa aaatcaatct gtggaatcgg gttgaggagt atttgcatta cactgtcaca
     2101 tagctacgct acaggaatat tgtagcatac ttgtacaacc tatatgagct tatcagggcg
     2161 ttatggtata actaattcgc ttagatataa cggtaagcct ctatttgtcc ctgcatttat
     2221 gtaacagaga aaaaagaatg ccagctcatg atggaaatag gatgatgtat tatgcataag
     2281 ttgtgcgatt aatactccta acttttgtat aaatgttttt ttattaatat gtattgaact
     2341 gcttttcttt tatttacaaa agaataaatg ataggactta tccagatatc aggaattatc
     2401 cagatgtcta ttggtgaagg tatttatacg aattttgata aatatttccg gtatcctgca
     2461 gctaatttca aatttactca ttttgcttta accggccaat cggcaggcag gttcaacatg
     2521 cactttttga gtatcaggta atcagatcca atcattgaat ttacggcact tcaggcagtg
     2581 ttaatataag ccggtgtata atcctctaga tacattccga agttctacga tctctcctac
     2641 ctctgatgtc atgtatatag tattgggaag aatggaactg ccgctaacca agagcgactt
     2701 acaaacactg aaaacataaa tattctgatt tgattaaacc gggagaaata gtaataccta
     2761 aggtacattc gttactaact caatcaggct ttttttgagg taaccggcta tcaaattgta
     2821 gttggctgta aggttatagt atgtattcaa tcgagaaagg cagccattta gactgccttt
     2881 cgatctgttt cacaagaaat ccaattattt actttatctt ctctaccttg atgatttccg
     2941 tcttgccatc aacataggta atatgtacaa agtaggttcc tgcactgtac ccggtcaggc
     3001 tcataccgcc ctgttgatct ttggcatcca ccttcatgag tacctttccg gtcaaatcaa
     3061 acactctgat ggtttcgttg ccactcagcc ctgttatatt aagatgacca tcagtaggat
     3121 tggggtacac ggatgcatga tttttaatat tgaaagtaac ggatcgtatg tcgctgtact
     3181 gatatttttc atcctgatcg atcattttca gtttgtacag attgagacct tctgcaggct
     3241 tatgatcaat gaaaaaataa tcttgccggg cgcccgaaat gccattggaa ggaacgaagg
     3301 tcactgtagt ccaaatccgg ccatcagtac ttctcaagac ttcaaacccc ctgaactgac
     3361 gctcgtcaag gctagtccag gacaaatagg ccgcagcttc cttccgcact acgtcaaagg
     3421 acaacagatt caaaggaagc ggggcattca gttgaatacc ataagtaaca aatactccgt
     3481 tagttccggc tttatctgtg atggtatatt caaagccgaa gggattgtgc gtattaccct
     3541 caccgtcctg accatatatt accatcttgt cagcattaaa attagaaatc accgaattgg
     3601 ccaatatttc gcggatgcca acaactccac caaaatcata gtacaatctg gtattgctgt
     3661 ggatagtccc tattgtaaat gttgttctga ttccaccctg attaccatta caagtgccgt
     3721 cggcggcgca atcttcaggg tcgctacccg ataaggaacc attggagtta ttgccggatt
     3781 ccttgagctt cggagaagac atcgtaatcg ccttgaaatt tgttataatc ggatacccgt
     3841 ttggcgggat agaactaaaa tccgaaatat tgacactata acttttgggg tctgcacccg
     3901 gcaactgttg aattccaaaa tcaacctcac cgacggacga ttcattgccc tggatattaa
     3961 tcgaaacctt tccatcatag cgggtcatcg ggctgttagc accagatcct gagccatcat
     4021 tatttccgct tcccaaggta ttattagtac caaatgtctc tccgacaaac gtccatcctg
     4081 aaggcagtga aggcaacgga gacgggcccc ctatcgtcgg taatgtcgtg gttaacaaca
     4141 gagtgatgtc ttgtttgatg ggaatattga tctgatatgt tccgtcattt cttactgaag
     4201 tataattggc tactttgcct tttttgtcaa cggcacaaac aaacaaaggc gggaggcctt
     4261 gctgaacata ggaggaggaa ggatatacag gattgccatt gactccattt ttattcagac
     4321 catcagcatc atgaaaaacc ctgcccgata acgaataggc tcctatgata ccaaaattga
     4381 taccattaat atcattgttg cctaaaacca aatttgaaaa aacggatggg tcggtacagt
     4441 ccggcacctt gcaaattgaa ttattggaat ggttattacc ggtatatttc cagccctcag
     4501 gcaatatttt atcagaactt cggacaattg aacctacaat taccttactt ttagtaacct
     4561 gaacactata tgtccctttc tgtgccgtta taatctcgaa ggtgccatta ggattgacag
     4621 ggacagagtt gtatacaact ccggaagctt ctatcaggtt ggcccacaac ccaatattac
     4681 ttccatatga gccaattgat tgtgattcgg cttgatcttt gattgcatcc aggtcatcat
     4741 ctatccatac tgtaccgcgg acagcattga tggtgcaagt ttctgtctcc agcttaatag
     4801 taacggacag cccggattca caggtgtgga agtcctgcac atgataattg agtgaaatgt
     4861 cacctacacc ctgaatttcc aggttatttc cattcagttg agcaattccg accggctctg
     4921 tatttattga taatactccg ttgccggggg cagcatttat atgcacggtt tgattcgtgc
     4981 agattttacc atgagttcct ttcatataat cgtcatcgac agaaattacc ggagcagtca
     5041 gtgcaccaca ggtttgcagg taggagttgc caaagacaaa atcctggtgg acctcgcctt
     5101 catcagttcc atcccctaat gaaccttcca cctggtggcc gacatatcca tacgtgccca
     5161 tacattgagc atcgactacg gttgtgtggc atccggactc gaccgcaaca atcttcatcc
     5221 cctgcaggga aggtggtatg ccgggattgg ttgcttttgc actattggtg agtccgtgta
     5281 ctcttctgcc gagcgcatat cccgactcca ggccccagtt gtacagggtg ctgtccctgg
     5341 tgattacgtt gacaaatgga tacttatagt cgtgctcatt gggagagaac cattgaatat
     5401 tgttcatgac agattcttca ctcactgcat atcttggttg tatccaggta ttcgaattac
     5461 ctatgctcca gttgcccaac tgaccaccca taccattccc gagcgcaaac aacctgccat
     5521 caccatagag aacgtagtag gtgatctccc ccgccgtaag tgaaccggtg gcgccaacca
     5581 tcttgataga accggaattg gaagggagga tcattttttc ggcaaaagcg cgcgattgtg
     5641 cagggttatt atctcctaaa taagtggagc gaccccatgt ccacaattgt ccttcggaat
     5701 ccagggcaat aaagcccgaa gcactccctc ttaaaactat aatattgtcc aatgatttgg
     5761 cttttgcacc ggcaccggtg cgaactctgg tccatgaatt gaatgaagtc agggtacttt
     5821 ggccccgcat gctttcgtgt tgtgccatta catatacatc accattgcag gtgagcatag
     5881 caagggcttc agtggtagca aacaaagtct ttacatccat agcatgaata cctcggggta
     5941 atataatcga ctgcatggaa tcggattttg ttacagcagc atggagcact gctccacgtt
     6001 tcccccaggc ccataattta tcatctgccg ttaagacaaa actttgtacg tttcgatgcg
     6061 ctttactgcc cattgcagct ttcaaaggcc tggccttaat gagggggaaa tgaaaagcat
     6121 caatgggtgt aggaatccat aatacggact ttccgttagg ggcggtattc tctccccaaa
     6181 ttcgaaatcc tccttccgga tctcgtaaaa tagaggtatg aaaagcagaa ataaagttat
     6241 catactctat ggaggcagca tcatgattgg atgctgatcc atagttggaa taattggtgc
     6301 cgggacagcc caaaccctca atatagccac attgggcatt tgcagcaata ggcacaacaa
     6361 aggtatttat cagaaagaag gcaaatccgg ccaaagtaaa acgtggccac ttcgcaggag
     6421 aataagacgt gttcattgca ataaattaag tgtgcgtcaa aaaaatatta tgcaaatata
     6481 taatttgttc ttatttcaac ctatcatcaa gataaatatt tttgtttttt ttaaattata
     6541 atattgtagt tatttaaaga ttat
//
LOCUS       DAOQMC010000128         6546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4954_length_6546_cov_4.143429, whole genome shotgun sequence.
ACCESSION   DAOQMC010000128 DAOQMC010000000
VERSION     DAOQMC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4954_length_6546_cov_4.143429"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PKV31_06245"
     CDS             <1..1328
                     /locus_tag="PKV31_06245"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNB92490.1"
                     /translation="RIVVTEKLKSIDDRPLKKAVSYEFYTLLDPSDKLPRIDDAALLD
                     SVQRRTFRYFWEFGHPVSGLARERNSSGDLVTSGGSGFGMMAIPVGIERGFITRRQGL
                     DRATQSVDFLLNKADRFHGAFPHWLSGTTGKVIPFSSNDNGADLVETSFLIAGLLTLR
                     EYFDGQQAEEVELRRDIDSIWQGVEWDWFRRDGKDILYWHWSPDKGWSMNLPISGWNE
                     CLITYILAASSPAHGIPPEVYHRGWASNGGIVNNQQAYGINLPLGSGTGGPLFFEHYS
                     FLGIDPRGLKDQYADYWEQVRAHTLINRAYCIANPRHYYGYGPECWGLTAGDVYNGYT
                     ASSPANDVSVIGPTAALSSMPYTPEESLDALHFFYYKLGDRLWGKMGFYDGFSLDRAW
                     FAESTLAIDQGPIVIMIENYRTALIWNLLMKAPEIKAGMEKLGFESPHF"
     gene            1522..2049
                     /locus_tag="PKV31_06250"
     CDS             1522..2049
                     /locus_tag="PKV31_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB92491.1"
                     /translation="MPKVKWIIDPVHSEIEFKVKYLMITNVIGRFDRFDMTVETEEED
                     FNTGKVWFSADVDSLETNDHERNGHLKSPDFFDIAAYPHIIFESGDMKVDGEVGEISG
                     NLTIKNTTKPVTFKVDFGGVTMDMYGTTRAGFSISTEIDRKEFGLRWNSILEGGGMMV
                     GDKVRISGEIQLVKR"
     gene            complement(2100..4397)
                     /locus_tag="PKV31_06255"
     CDS             complement(2100..4397)
                     /locus_tag="PKV31_06255"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNB92492.1"
                     /translation="MKKWFLLKSLLLTAHFIYAQLNREPVDFVNPLMGSLSTYELSSG
                     NTYPDISMPWGMNSWTPQTAKMGDGWQYYYNANKIRGFKQTHQPSPWINDYGQFSIMA
                     VTGGPKFRERDRMSWFSHKAETATPYYYSVYLADHDVTAEFAPTDRACIFRFTFPEND
                     QSFVVIDAFDKGSYIRINKKERKIIGYTTRNSGGVPDNFKNYFVIQFDQPFDVAYTFR
                     DSILVKDKHDVTANHAGAVIGFKTHRGEKIIARVASSFISYEQASLNLSRELGDKGFE
                     VIKQQGRDIWNKELSRILVEGGSDDETKTFYSCLYRTMLFPRKFYEYDIDNKVMHYSP
                     YNGQVLPGVMYTDNGFWDTFRAVFPFFNLMYPELNSEIMQAMVNVYKESGWLPEWQSP
                     GHRNCMIGSNSASIIADAYIKGIRGFDVETLYQAILKNSNSQGPLQSVGRAGAEEYNE
                     LGYVPYDIGINENAARTLEYAYDDFAVSQLARALKKPDEEVSLFTLRAQNYHNVYDPS
                     TRLMRGRNKDGSFQSPFNPFKWGDAFTEGNSWHYTWSVFHDVNGLAMAMGGRDTMAMM
                     LDSVFSLPPIFDESYYGQVIHEIREMQIMNMGQYAHGNQPIQHMIYLYNYLGQPWKAQ
                     YWLHQVMNRLYNPHPDGYCGDEDNGQTSAWYVFSAMGFYPVCPGTTQYVIGSPLFKRI
                     TLSLSNDKKVQINAPAVNKENVYIQDISLNARPYDKLYLDHFELMKGGKFDFIMTNIP
                     NKTRVYKEAQLPFSMTKWPEPKKKR"
     gene            4758..>6546
                     /locus_tag="PKV31_06260"
     CDS             4758..>6546
                     /locus_tag="PKV31_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB92493.1"
                     /translation="MKVLYFSLFMTLPYLMQAQYQYPPARMSDQTDNYHGEIIRDPYR
                     WLEDDHAEETKNWVIMENEVTASYFEKIPYIDELTHRLNALYNYPKYSSPFRKGDYYY
                     YYYNSGLQNQSVLYRTKGLNGTPEIVLDPNTFSADGTTKLAGFSLSKNGDYAAVYFSK
                     GGSDWSEAKILDMKSLKYLGDTLSWIKVSGAAWQDDGFYYSRYPAPEGSALASKNENH
                     KVYYHRIGQSQDKDRLIFEDPASPQSFNIASTTDDEQYTILSRSDRGKGLDGNALFFM
                     KKGEKTFRPIVSEISDFDYSFIDNYGDDFVIVTNAGAPNKKVMIFNANTGKWKTIIQE
                     KKEPLQAVSSAGGKLFVSYMQDVSTHVYVYDINGKQENEILMPGPGNAGGFGGERNDK
                     TIFYTFTSFTYPSVIYSYNIASGKSTVYRKSEVDFNSDDYETRQVFYPSKDGTKIPMF
                     IVYKKGLTLNSANPTLLYGYGGFNISLNPGFSPLLIPFLEQGGVYAQANLRGGGEYGE
                     AWHEQGMKLKKQNVFDDFIAAGEYLIREKYCNSEHLALRGGSNGGLLVGAVINQRPDL
                     AKVAIPQVGVMDMLRFQKFTIGWNWIADYG"
BASE COUNT         1860 a         1476 c         1469 g         1741 t
ORIGIN      
        1 atcgcattgt agtaactgaa aaactgaaga gcatagatga caggcccttg aagaaggccg
       61 tttcctatga attctatacc ttgctggacc cgtcagacaa acttccaagg attgatgatg
      121 cagcgttgct ggatagtgta caacgccgta ctttcagata cttttgggaa ttcggacacc
      181 cggtaagtgg tcttgccagg gaacgtaatt catccggtga tctggtgaca agtgggggaa
      241 gcggcttcgg gatgatggcc atcccggtgg gcatcgaacg cggtttcatc acacgccggc
      301 aagggttgga ccgcgccacc caatcggtag actttctgtt gaacaaggcc gatcgcttcc
      361 atggggcatt tccacattgg ctgagcggca ccaccggtaa ggtgatacct ttcagctcca
      421 atgacaatgg tgctgacctg gtggaaacct cttttctaat tgccggatta ctgactctca
      481 gagaatattt tgacgggcaa caagctgaag aggtggaatt gcgccgcgat atagactcta
      541 tatggcaggg tgtggaatgg gattggttta gaagagatgg caaggatatc ctgtactggc
      601 attggagtcc cgataagggt tggtcgatga atctgccaat atcagggtgg aatgaatgcc
      661 ttatcacata catccttgct gcatcaagtc ctgcacacgg aattccaccg gaagtgtacc
      721 acaggggttg ggccagcaat ggcggcatcg tcaataacca acaagcctac ggaattaatc
      781 tcccattggg ttcaggtacc ggaggtccgt tatttttcga gcattattct tttctgggca
      841 tcgacccccg gggcttgaag gaccaatatg ccgactattg ggagcaagtg agggcacaca
      901 ccctgatcaa cagggcatat tgcatagcaa acccccgtca ttactacgga tatgggcctg
      961 aatgctgggg ccttactgca ggagacgtat ataacggata tacagcaagc agccctgcca
     1021 atgacgtcag cgttatcggc cccactgctg ccttatcctc catgccctac acccctgaag
     1081 aaagccttga tgccctccat tttttttatt acaagctagg ggacaggctt tggggtaaaa
     1141 tggggtttta cgacgggttt tcactcgatc gtgcctggtt tgctgagtct acccttgcca
     1201 tcgatcaggg gccgattgtg attatgatag aaaattatag gacggctttg atttggaatc
     1261 tcttgatgaa ggcaccggaa ataaaggcag gcatggaaaa acttggattt gagagccccc
     1321 atttttaggg tttcaaacaa acttacagca ggaaataata aaaatcgact tcccttcaat
     1381 caattagcct catcatacac gggaattttt gaagatagca cgttttatca agcacggaaa
     1441 atatccaaat attctatacc tttatgaact tttagtccga actttctatt agataataac
     1501 catataaatg aatataccgt catgccgaaa gtaaaatgga tcatagatcc ggtacatagt
     1561 gagatagaat ttaaggttaa atatctaatg attaccaatg tcatcggccg gtttgatcgt
     1621 tttgacatga cggtcgaaac ggaagaagag gattttaaca ccggaaaggt ctggttttca
     1681 gctgatgttg acagcctgga gaccaatgac catgaacgta acgggcattt gaaaagtccg
     1741 gatttctttg acattgcagc ttatccccac atcattttcg aatcaggtga tatgaaggtt
     1801 gacggtgagg taggtgagat ctccggcaac cttaccatca aaaacaccac aaagcctgta
     1861 accttcaagg tggacttcgg tggagtgact atggatatgt atggcactac tcgagccgga
     1921 ttctctatat ccacggaaat agaccgcaag gaattcggcc ttcgatggaa ctccatcctg
     1981 gaaggtggtg ggatgatggt cggtgacaag gtcaggatct caggagaaat ccaacttgtc
     2041 aaaagataat gacaggtccc ggtcttcctt tgttaatcca ttaataccgt ctgcggtccc
     2101 tatcttttct tctttggttc cggccatttc gtcattgaaa atggcagttg cgcttcctta
     2161 taaaccctgg ttttattcgg aatattggtc atgataaagt caaacttccc tcctttcatt
     2221 agttcaaaat gatcaagata cagcttgtcg tatggccggg cattgagtga tatgtcctga
     2281 atatatacat tctccttatt gacagcaggc gcattgatct gcactttttt atcgttgcta
     2341 aggcttaaag taatcctttt gaaaagggga gaaccaatga catattgagt cgtaccgggg
     2401 cataccgggt agaagcccat agcactgaac acataccatg cactggtctg gccgttgtct
     2461 tcatcaccgc aatagccatc cgggtgtgga ttgtaaagcc tgttcatgac ctgatgcagc
     2521 cagtattgag ccttccacgg ttgtccaaga taattataca gatagatcat gtgctgtata
     2581 ggttggtttc catgagcata ctggcccatg ttcataatct gcatctccct gatttcgtgg
     2641 attacctggc cgtaataact ttcgtcaaat atcggtggca ggctgaagac actatcaagc
     2701 atcatagcca tggtatccct gccgcccatc gccatcgcca gtccgttgac atcatgaaac
     2761 acactccagg tgtagtgcca gctgttaccc tccgtaaagg catcacccca cttgaatgga
     2821 ttgaagggtg attggaagct gccatcctta ttacgcccac gcatcaacct ggtcgatgga
     2881 tcatagacat tgtgataatt ctgtgcgcgc agggtaaata gagaaacctc ctcatcgggc
     2941 ttcttaagcg cgcgcgccaa ctgtgacaca gcaaaatcat cataggcgta ttccagggta
     3001 cgtgctgcat tttcattgat tccaatatca tagggcacat aacccaattc gttgtactct
     3061 tccgcacctg cacgacctac agactgaagc ggcccctgac tgttgctgtt ttttaatatc
     3121 gcctgataca gcgtctcaac atcaaatcct cttattccct tgatgtatgc atcggcgatg
     3181 atggaagcag agttgctacc gatcatgcaa ttcctgtgcc ccggactctg ccactccggc
     3241 aaccaaccac tttccttata tacattgacc atcgcctgca tgatctcact gttcaattca
     3301 ggatacatca ggttgaagaa tggaaataca gcccggaatg tatcccagaa accattgtct
     3361 gtgtacatga cgcccggcaa tacctggccg ttgtaaggac tatagtgcat cactttgttg
     3421 tcgatatcgt attcatagaa cttccgtgga aacaacatgg tcctgtacag acaggaataa
     3481 aaagttttgg tctcatcatc actgcctccc tcaaccagga tacgactaag ctctttgttc
     3541 cagatatccc ggccttgttg cttgatcacc tcgaagccct tgtcacccaa ctccctcgac
     3601 agattgaggg aagcctgttc ataactgatg aaggatgacg ctacacgtgc gatgatcttc
     3661 tcgccacgat gtgtcttgaa tccgataaca gcacctgcgt gattggcagt tacatcatgc
     3721 ttatccttca ccaatatact atcccggaag gtatatgcga catcaaatgg ctgatcgaat
     3781 tgaattacaa aataattctt gaaattatcc ggcacacctc cactattgcg ggtcgtatat
     3841 ccgattatct tcctctcttt cttgttgatc ctaatgtagg aacctttatc aaaagcatct
     3901 atgaccacaa aggactgatc attttccgga aaggtaaagc ggaatataca ggcccggtcc
     3961 gtaggtgcaa actctgcagt cacatcatgg tcggccagat aaacactata gtaatatgga
     4021 gtagcagttt cagccttgtg ggaaaaccag gacattcgat ctctttctct aaacttcggc
     4081 cccccggtta cagccatgat tgaaaactga ccatagtcat tgatccaggg agaaggttga
     4141 tgagtctgct tgaaccctct tattttattg gcattgtaat aatattgcca tccatctccc
     4201 atcttggcag tctggggtgt ccagctgttc ataccccatg gcatcgagat atccggataa
     4261 gtattaccgg aagatagctc ataagtggac aaggaaccca tgagaggatt gacaaaatca
     4321 accggctctc tattgagttg agcataaata aaatgtgccg tgagaagcaa agatttgaga
     4381 agaaaccact ttttcatgaa aaaaacctaa agcaatgaaa ttaaaaataa gaaaagtcaa
     4441 tacttgtacg aatgtataaa aagaaaacag aatccgaccg gctaaaattt tcacacctgt
     4501 taataacaca ctaccggcac gcattgtacc tttgattaac aggaaaatca gattgactgt
     4561 ttttccaaat agggtatttg aagatcaagc aaaaaaaaga gagtgaactg aaataatcaa
     4621 ccaggtcaat ccttatattg gatcaacact atctctttcg tatcactgat attcaccggc
     4681 tattcccctg gtgaaaactt aggatattta tgtattctgg tgtaaaaaga ttacctttat
     4741 agccaaaatt ttttcagatg aaagtacttt atttttcttt attcatgaca ttaccatacc
     4801 ttatgcaggc acagtatcaa tatccacccg ccaggatgag cgaccagacg gacaattatc
     4861 atggagaaat catccgggat ccttaccggt ggctggaaga tgaccacgct gaggaaacca
     4921 agaactgggt aatcatggaa aatgaagtta ctgcaagtta ttttgaaaag atcccttata
     4981 tcgacgaatt gacccatcgg ctaaatgcac tttacaacta tccgaaatat tcctctcctt
     5041 tccgaaaggg cgattattat tactattatt acaattccgg attgcaaaat caatcggtat
     5101 tatacaggac aaaaggtctg aatggtacac cggagatcgt tctggaccca aacacatttt
     5161 ccgctgatgg caccaccaag ctggccggat tttcactctc caaaaatggt gattatgctg
     5221 cggtatattt ctcaaaggga ggctccgact ggagcgaagc aaaaattctg gacatgaagt
     5281 ccttaaaata ccttggcgac acgctaagct ggataaaagt atccggtgcg gcctggcagg
     5341 atgatggatt ttactacagc cgatatccgg caccggaagg cagtgccctg gcctcaaaaa
     5401 acgaaaacca caaggtctac tatcatagga ttggacaatc gcaggataag gatcgcctga
     5461 tatttgaaga cccggcaagt ccacaaagct ttaacatagc atccaccaca gatgatgaac
     5521 aatacactat cctttcacga tccgaccggg gaaaaggatt ggacgggaat gcactctttt
     5581 tcatgaaaaa aggagaaaag acctttcggc caatcgtgag cgagatatct gactttgact
     5641 attccttcat cgataattat ggtgatgatt ttgtaatcgt aaccaatgcc ggggctccga
     5701 ataaaaaagt aatgatattt aatgcgaata ccggaaaatg gaaaacaatt atccaggaaa
     5761 aaaaggaacc tcttcaggct gtaagttctg cgggaggaaa actttttgtc agttatatgc
     5821 aggacgtctc tacccatgta tatgtatacg atatcaatgg caagcaggaa aatgaaatcc
     5881 tgatgcccgg tccgggtaat gcagggggct tcggtggtga gcggaatgat aagactatct
     5941 tttacacatt cacgtcattt acctatccat ccgtcatcta ttcatataat attgcttccg
     6001 ggaaatcgac tgtgtacaga aaatccgaag ttgatttcaa ttctgacgat tatgagacca
     6061 gacaagtgtt ctatcccagc aaggatggca ctaaaatacc gatgtttatc gtgtataaaa
     6121 aggggctcac gctcaacagc gccaacccta ccctgctcta tggctacgga gggtttaata
     6181 tcagtctcaa tccgggcttc agccctttgc tgattccatt tcttgagcaa ggcggtgtct
     6241 atgctcaggc taatctcaga ggaggtggag aatatggcga agcctggcat gaacaaggca
     6301 tgaagctcaa gaagcagaat gtgttcgatg atttcatcgc tgccggtgaa tatttgatca
     6361 gggaaaaata ttgtaactcg gaacatctcg cgcttcgtgg aggctccaac ggcggattgc
     6421 ttgtcggtgc agtcatcaat cagaggccgg atctggctaa agtagccatt ccacaagtgg
     6481 gcgtgatgga tatgttgcgt tttcaaaaat ttaccatcgg ctggaactgg attgcagact
     6541 atggtt
//
LOCUS       DAOQMC010000129         6519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_4989_length_6519_cov_5.045792, whole genome shotgun sequence.
ACCESSION   DAOQMC010000129 DAOQMC010000000
VERSION     DAOQMC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_6519_cov_5.045792"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..322)
                     /locus_tag="PKV31_06265"
     CDS             complement(83..322)
                     /locus_tag="PKV31_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92494.1"
                     /translation="MRVGIGFAYIVLCIDACCIQIFLDGFLQAAGMCGQGFMLGPGVA
                     ELLTRIVSGKTEPADEEILSYLSPYRQFVSEEKLK"
     gene            541..945
                     /locus_tag="PKV31_06270"
     CDS             541..945
                     /locus_tag="PKV31_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92495.1"
                     /translation="MKKIIICLFAAIALTSCKSDSKNETSATDTAATADAAAGKDMKD
                     SLILTQVPTFTDQATINFSAEYDSFLSEYRKAVRENNTEATQKLLDKYLDLAKKAAEI
                     SEKLNDEERSKFQQFITSRQAEYAEITSGDKF"
     gene            1129..1491
                     /locus_tag="PKV31_06275"
     CDS             1129..1491
                     /locus_tag="PKV31_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92496.1"
                     /translation="MRFLALFFVVLFAFASCKKAEKTEAAPAMEAATEAPAVPQTANV
                     DVNAYLQEFNTLLTTYKEALAKKDTAMLSNLSMKYTELAQAGMEAAEKATGEDKEKML
                     SSIQMMSDQFKAISEGKK"
     gene            complement(1544..3178)
                     /locus_tag="PKV31_06280"
     CDS             complement(1544..3178)
                     /locus_tag="PKV31_06280"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNB92497.1"
                     /translation="MKIALAQLNYIVGDFSGNHEKIIDNISMARQQGADLIVFAELAV
                     CGYPPEDLLEYRSFLQASEDAIRSLKLHSGDIGIIIGAPSLNHQRGKAYFNSAFFISE
                     GEVKEIVHKALLPEYDVLEEPRYFAPGRDFKVIEFRGKRIALTVCEDMWNIGNENPLY
                     DVCPMDMLIEQRPDFAVNISAVNFYIGKASLRKKVITSNAVKYKIPFFTVNHIGAQTG
                     LIFDGGSSVASPDGLIFREMPCFEEALEVFDLDEVIKGNHEDEVVMGEIELIHKALIV
                     GIQDYFKKQNLSSAILGLSGGLDSAVVAALATEALGKENVRGLLMPSQYSSSHSLEDA
                     ISLAQNLGIRFDIVPIKDIFDQYIFTLQPLFAGMPENVTEENLQARIRAMLLMAVSNK
                     YGNILLNTSNKSEVAVGYGTLYGDVCGAISVLGDVYKTEVYRLAHFINRDRIIVPLNT
                     INKAPSAELRPGQKDSDSLPPYEVLDQILYEHIENGKGSTEIVELGFDAQMVENILRK
                     VKFNEFKRRQAPPVLRVSRKAFGLGRKMPIVNGFSM"
     gene            3434..4585
                     /gene="dnaX"
                     /locus_tag="PKV31_06285"
     CDS             3434..4585
                     /gene="dnaX"
                     /locus_tag="PKV31_06285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNB92498.1"
                     /translation="MAQFIVSARKYRPQRFKDVLGQEHISHTLKMAFSQDKLAHAFLF
                     TGPRGVGKTTCARILAKALNCTGRTGDYEPCGKCESCISFDDNASFSIFELDAASNNS
                     VEHIRSLVEQVRIPPLAGKYKIFIIDEVHMLTTAAFNAFLKTLEEPPPYAVFILATTE
                     KHKILPTILSRCQIFDFRRIQVFDIVKQLSLVATEENIIAEEEAMHLIATKADGAMRD
                     ALSIFDRIAGATNNHITYQAVTNNLNLLDYTYFFKVTDQLITEDLPSVMLTIDEVLKK
                     GFDPEVFLSGLAEHLRQLLVSRDRATVKLIENTESVRERYAQQAALLPLDYILSALQL
                     CSECDINFRTAKNKRLHLEMTMIKMVYLPALIENGGINTPEKKKSLNPV"
     gene            4663..5196
                     /locus_tag="PKV31_06290"
     CDS             4663..5196
                     /locus_tag="PKV31_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92499.1"
                     /translation="MAKTEQPDSMPAKTVQINMDDDGSAKQEKTPVSRKDRLQQFVSR
                     AKTEIEKTANSYQPFTVEQARKLLATYASTASSTIRIILNERIADSKEENKLCFTVAS
                     NYEKENLLNEPGLLDYLRKNLNNQLITLEFEIDSSLMGDANDMKPISTEEKIKILVER
                     NALLQDFMEKLNLKIDE"
     gene            complement(5259..>6519)
                     /locus_tag="PKV31_06295"
     CDS             complement(5259..>6519)
                     /locus_tag="PKV31_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92500.1"
                     /translation="IDIFGHHYGVYASPRENQVFAETNVGVSGTNYLQSGLAMNGHVN
                     FDLNNYRYYGGYNPGDTLFPSDPAKRRFTNIGAGLKIFNSKKNTLDLDYWAGADMYGY
                     KDNFVSTERGFSLDIGATKWIVDRHNFTLQITDHQLTYGDTEKGHVNNVNVKPSFTFH
                     GDKVKVKLGGNFVGSNGFAFFPDVEASANITGPAINVFVGAYGDLYQNSFKRLSTINP
                     FIYTRFAETGENGPFNTKYYDFYGGVGGGFNSVNYRLKIGYKTNKDYALFVQQKEDLR
                     QFDVIKDDVNIFYIGGELSASFFEKLNTSLHFLQQSASPRVQAKAYGIVPFEIGLNAS
                     YMMLEDKFKLKGDLNFAAGSPYRNRFDSVGNLNPMFDVSIQGDYFFTDNFGAFLMVNN
                     LASVKYQRWYRYPTYGINILGGISLRF"
BASE COUNT         2040 a         1498 c         1253 g         1728 t
ORIGIN      
        1 accggtagcc gagtgcctgc tggttatctt accgggagtc aaacttatac tcgatagcct
       61 gaaaaaataa gcccgctggc tgctatttta atttttcttc gctgacaaac tggcgatatg
      121 gggaaagata agaaagaatt tcctcatcag ccggttcggt ctttcccgag acgattctgg
      181 ttaaaagctc agctactccc ggtccaagca taaaaccctg gccacacatg ccggccgcct
      241 gcaaaaaacc atcaaggaat atctggatgc agcaggcatc aatacagaga actatgtatg
      301 caaatcctat tccaacacgc aaatggtaaa tgcagaaccc aaaaacgaag cggaggctaa
      361 gatgaacaga cgtgtggaga taaaaattct ggaataggag ataaaaaatc cggaatcaag
      421 acataaaaaa tgctcgattg atgtgaccgt tttcaaaaag taccgtcaaa attcggttgg
      481 ctcgctgtac tgctatttat agcaaaatgt tatctttggc cccaaaatgt aaagtataat
      541 atgaaaaaaa taataatttg tttgtttgct gccatcgcct taaccagctg caagagtgac
      601 tctaaaaatg aaacttcagc cactgacaca gcagctactg ctgatgcagc tgcaggaaag
      661 gacatgaagg actccctcat ccttactcag gtgccgacct ttactgatca ggcaaccatc
      721 aacttttcag ccgaatatga ttcgtttcta agtgaatatc gtaaagctgt aagagaaaac
      781 aatacggaag ctactcaaaa gttattggac aaataccttg acctggctaa gaaagctgct
      841 gaaatatctg aaaaactgaa tgatgaggag agaagtaaat tccagcaatt cataacatca
      901 cgacaggccg aatatgctga aatcacttca ggagacaaat tctgatattc aaaagataaa
      961 ccaacggaaa gcaaattgcc ctatatgttt aactttttcc atgaatacac aaccgtctcc
     1021 atgattgaaa cacaccggca taatgctcaa taatatgttc aatcatcttc gggacgatgt
     1081 catgttaggg aagatatttt tttattaata gtttataatt aattaccaat gagattctta
     1141 gctttatttt ttgtagtact ttttgctttt gcaagttgta aaaaagcaga aaaaaccgaa
     1201 gcagcaccgg caatggaagc tgcaacagaa gcccctgctg tacctcaaac agctaatgtg
     1261 gacgtcaatg catacttgca ggaatttaac acgttgctga caacatacaa ggaagctttg
     1321 gcaaagaaag acactgccat gctcagcaat ctttccatga aatatactga actagcccag
     1381 gccggcatgg aggctgctga aaaggcaaca ggagaggata aagagaaaat gctttcctcc
     1441 attcaaatga tgtcagatca atttaaggct atttcagaag gaaaaaagta attttcgtta
     1501 aagcaaataa aattcagccc ggagatttct tcgggctgtt ttgttacatc gaaaacccat
     1561 tgacaatggg catcttcctg cccagaccga atgcctttct tgataccctc aatacaggtg
     1621 gagcctgacg ccgcttgaac tcattaaatt tgacttttct tagaatattt tcaaccatct
     1681 gagcatcaaa acccaattcc acaatctcag tactcccctt gccattttca atatgctcat
     1741 aaaggatttg atccagaact tcataaggag gcagactatc actatccttt tgtccgggtc
     1801 gcaattccgc tgaaggagcc ttgttgatag tgttcaatgg cactatgatc ctatccctgt
     1861 taataaaatg cgccaggcga tacacttctg ttttataaac atcaccaagc actgaaatcg
     1921 ccccacacac atcgccgtaa agtgttccat agccaactgc cacctcactt ttattgcttg
     1981 tattcaataa gatatttcca tacttattac tgactgccat gagcaacata gctctgatgc
     2041 gcgcttgaag gttttcctcg gtcacatttt ccggcatgcc tgcaaacaaa ggttgtaacg
     2101 taaaaatata ttgatcgaaa atatccttta tcgggacaat atcaaacctg ataccgagat
     2161 tttgagctaa tgaaatagca tcttctaaac tgtggctgct gctatactgt gaaggcatta
     2221 gcagccctct gacgttctcc ttccccaacg cctcagtcgc caacgctgca acaactgcac
     2281 tgtccaatcc tcccgacaga cctaaaatag ctgaagataa attctgtttt ttgaaataat
     2341 cctgaattcc aactatcaaa gccttatgta tcaactcaat ttcacccatt accacctcat
     2401 cctcatgatt accctttatt acttcatcca aatcgaaaac ttcaagcgcc tcttcaaaac
     2461 agggcatttc ccggaaaatc aaaccatccg gtgaggccac agatgagcct ccgtcaaata
     2521 tcagcccggt ctgagcacca atgtggttta cggtaaaaaa tggaatttta tattttacag
     2581 catttgaggt aatcaccttc ttgcgaagac ttgccttccc aatgtaaaaa ttgaccgcgc
     2641 ttatattgac agcaaagtca ggacgctgtt cgatgagcat gtccatcggg cagacatcgt
     2701 acaaaggatt ctcattacct atgttccaca tgtcttcaca tacggtcagg gcaattcttt
     2761 tgccgcggaa ttcaattact ttaaaatccc ttcccggggc aaaatatctc ggctcctcca
     2821 aaacatcgta ttcaggcaac aacgccttat ggactatttc cttaacttca ccttcactga
     2881 taaaaaatgc actattgaag taggctttcc ctctttgatg gttgagagag ggtgcgccta
     2941 ttatgattcc gatatctccg gaatgcagtt tcaaggaccg gatggcgtct tcactggctt
     3001 gaagaaacga ccggtattcc agaagatcct ctggcggata accgcatact gccaattcgg
     3061 caaatacgat aagatctgcg ccctgttgcc tggccattga gatattgtca ataatctttt
     3121 catggttgcc ggaaaaatct ccgactatgt aatttaattg cgctaatgct attttcattc
     3181 ttaataatta ttcagtagag accgatttgc taaggaatag cagaatcggt aaacaaagtt
     3241 aagtctacct cgtttattta aaagaaaaac atttgatgca tctgtgtttt ttttaactgc
     3301 ctgttgcagt gaaatagtcg gataacccaa atgaacaatc tatttgctat ccgacattct
     3361 ataatattcc gaaaaataat cagtcagatt tggcataatt ttcaaataaa ctatatactt
     3421 tcaccctcca tttatggcac aatttatcgt ttcagcaaga aagtataggc cacagcgctt
     3481 caaggatgtc ctgggacagg aacacatctc ccataccctg aagatggcgt tttcgcagga
     3541 taagcttgct cacgcctttc tattcaccgg gccccggggg gtcggcaaga cgacctgtgc
     3601 ccgtattctt gccaaagcat taaattgtac cgggaggaca ggtgattatg agccatgtgg
     3661 caaatgcgaa tcatgcattt catttgacga caacgcctcc ttctcgatct ttgagttaga
     3721 tgcagcgtcc aacaacagtg tggaacacat cagaagcctg gtagaacaag tcagaattcc
     3781 accgttggca ggtaaataca agatcttcat catcgatgag gtacatatgc tgaccactgc
     3841 agccttcaat gccttcctca agacgctgga agagccacct ccctatgcag tatttatcct
     3901 ggcaaccacg gagaagcaca aaatcctgcc taccatatta tcaagatgcc agatttttga
     3961 ctttcggcgc atccaggtgt tcgatatcgt aaaacagttg agccttgttg ccacagagga
     4021 aaacattata gctgaagaag aggccatgca tctcatagca acaaaggccg atggagccat
     4081 gagggatgcg ctttcaatct tcgacagaat agccggagca accaacaatc atatcactta
     4141 ccaggcagtc accaacaatc tgaacctcct tgattataca tactttttca aagtgacaga
     4201 tcagttgatt actgaagatc tgccctcggt catgctaact atcgatgagg tcttgaaaaa
     4261 agggttcgat ccggaagtat tcttatccgg tctggcagaa catctaaggc aattgcttgt
     4321 cagtcgggac cgggcaactg tcaagcttat tgaaaacacc gaatcggtac gggaaaggta
     4381 cgcacaacaa gcagctttat tacctcttga ttatatcctg tcggcacttc agctatgcag
     4441 tgagtgcgat attaatttca gaacagcaaa aaacaaacgt ctgcatctgg aaatgaccat
     4501 gataaaaatg gtctatttgc ctgccttgat tgaaaatgga ggaataaaca cccctgaaaa
     4561 aaaaaagtcc ctgaacccgg tctgataaaa actaccctac cggaacaaaa tacctcaatc
     4621 gtcccaccaa tatcatcaag taccattgaa gaaaattatg gtatggcaaa aacggaacaa
     4681 ccggattcta tgccggctaa aaccgttcaa atcaatatgg atgatgatgg atcagccaaa
     4741 caggagaaaa cgcctgtttc cagaaaagac agattgcagc agttcgtttc ccgggccaaa
     4801 acagaaatag agaaaacagc caactcttat cagccgttta ccgttgagca ggcacgaaag
     4861 ctattggcaa cctatgcctc aaccgcatcg tccaccatcc gaatcatcct aaacgaaaga
     4921 attgcggata gtaaggaaga aaacaagctc tgctttacag tagcttccaa ctacgaaaag
     4981 gaaaacctac tcaatgaacc ggggcttttg gattatttga ggaaaaatct taataatcaa
     5041 cttataacac tcgaattcga gattgattcc tctttgatgg gagatgccaa cgatatgaag
     5101 cctatctcca ccgaggaaaa aataaaaatt ctagtggaaa ggaatgccct actgcaagat
     5161 ttcatggaaa aactcaacct caaaatcgac gagtaattcc ccttgtctac ctgacccacc
     5221 gacacatccg ttgtgcaggg ctgaagtaat attaaccatc aaaatctcag actaatgcca
     5281 cccaatatgt tgataccata ggttggatat cggtaccagc gctgatactt gacagatgcc
     5341 agattattga ccatcaggaa ggctccgaaa ttgtccgtaa agaaataatc gccttgaata
     5401 ctgacatcaa acataggatt aaggttgcct accgaatcaa atctattcct gtatggactt
     5461 ccggccgcaa aattcaaatc acctttaagt ttaaacttgt cctccagcat catataagaa
     5521 gcattgaggc cgatctcaaa tggaacaatt ccataggcct ttgcctggac acgaggagat
     5581 gcggactgtt gcaggaaatg gagggaagta tttaattttt caaagaagga tgcagacaac
     5641 tcgccaccga tgtagaaaat atttacatca tctttgatga catcaaactg cctcaaatct
     5701 tccttttgct gaacaaaaag cgcatagtcc ttattggttt tgtagccaat cttcagtcga
     5761 taattcaccg agttgaagcc gccacctaca ccaccgtaaa aatcataata cttggtattg
     5821 aaaggtccat tctcaccggt ctctgcaaat cgtgtataaa taaatggatt gatggtactt
     5881 aatcgtttga agctgttttg ataaagatcg ccataagcac cgacaaaaac attgatggcc
     5941 ggtccggtta tgttggcact ggcttcaaca tccgggaaga atgcaaatcc attgctccct
     6001 acaaagtttc caccgagctt caccttgacc ttatccccat gaaaggtaaa ggaaggtttg
     6061 acattcacat tattcacatg gcctttttca gtatctccgt atgtcaattg atgatctgta
     6121 atctgaaggg tgaagttgtg cctgtccaca atccactttg ttgcaccgat atcaagtgaa
     6181 aaccctcgct ccgtcgatac aaagttatcc ttatagccat acatgtccgc tccggcccaa
     6241 tagtcaagat caagtgtatt tttcttgcta ttgaagattt tcagtccagc accaatattg
     6301 gtaaacctcc tttttgccgg atcactcggg aatagggtat cgcccggatt atagcccccg
     6361 taataacggt aattattgag gtcaaagttg acatggccat tcattgcaag cccggattga
     6421 agataattag tcccgcttac accgacattt gtttcggcaa aaacctggtt ttctcttggc
     6481 gatgcataaa ccccgtaatg atgaccaaaa atatcgatg
//
LOCUS       DAOQMC010000130         6430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5088_length_6430_cov_5.131294, whole genome shotgun sequence.
ACCESSION   DAOQMC010000130 DAOQMC010000000
VERSION     DAOQMC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5088_length_6430_cov_5.131294"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PKV31_06300"
     CDS             <1..1060
                     /locus_tag="PKV31_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB92501.1"
                     /translation="IAIILAIFFIYGVSRYFLINHWRSYTDAIFYPTKEEIKIKESTL
                     RLTEKLDLNMASSHQLVQIGLPQNISNRISRYRDKIGGFKDWQQVERTYGITKEQLDI
                     LHQYCSIRLASKKRHNTQTSYTPNHFHKKKKGVHIPSLLPFDPNTVELKDLEAMGIRP
                     SIARGIVNFRKSGFIYRNKEDLLKIYAMDEVTFEKLKPFIKLEKQKVETQTLKKRSET
                     IIDPVDINRATVESLSRLPGIGKYYSRLILNWRDKLGGFHRIEQIASTYHLPDSIFSK
                     IAVYITFDTPPNRIDINKADSETLAKHFFIRAKEAKIIVAYRDNHGPYQTVEDLLKTG
                     SIDRDWLEKAGPYLIAGK"
     gene            complement(1068..1838)
                     /locus_tag="PKV31_06305"
     CDS             complement(1068..1838)
                     /locus_tag="PKV31_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92502.1"
                     /translation="MVKTILLTGATSGIGKATARELSAMGRYRLILTGRRKERLEALK
                     EELIRSDKTEVHVFAFDIRDLNACKVFWDGLPDGLKQIDILINNAGLAKGLSEIHEGS
                     FDHWEQMIDTNIKGLLHMTRLISPGMVERRQGHIINLGSTAGKETYPNGNVYNATKFA
                     VEALTRAMRMDLYKYGIRVSSVSPGAVEETEFALVRFDGDAQRARIYDDYTPLKAKDV
                     AEIIAFILSRPPHVNIQDVLVTSTQQASSMLFDKTGRK"
     gene            complement(1832..3151)
                     /locus_tag="PKV31_06310"
     CDS             complement(1832..3151)
                     /locus_tag="PKV31_06310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB92503.1"
                     /translation="MLSKWWKVVLLPLLVIYIFNSCLVTNQKFDKLMPGIWRGELYLD
                     ESNPLSRNISKVGNDQDISFKNAFTEGVLPFNFEVTYEGDSMFVDFINGDNRVRAEET
                     LWGFNRRSGKDTIRINFVAYDTYLRAEVEDGIMTGAWYNPRKNWRVPFRGLAGKDYRF
                     TMVNEKPSFDISGKWDVIFHFENRNDTTIGQFTQDNNSLKGTFASPTGDYGFLEGTVQ
                     GDRMFLSGFAGGAAYLVSGKITEKGRMKGTFMSGLTSRATWEAVFNPDAILPDASILT
                     RASGNQAVNFTFPDEQGNLVSLSDPTFNGKAKVLTITGTWCPNCKDEARFLTEYLNKR
                     QNDRLSVIALSFEAFDDTAKVRSLLSNYKKVLDLPYTVLWAGKPGPEASSRVPFISGI
                     KAYPTMIFLDRNNHVVSVHTGFSGPATDEYEAFKKDFDLQVEKLTKW"
     gene            complement(3202..3729)
                     /locus_tag="PKV31_06315"
     CDS             complement(3202..3729)
                     /locus_tag="PKV31_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92504.1"
                     /translation="MELYGVGSRLSHPSYGDGVVIKLNPENYEICFMTYGIKQISKDY
                     DRFSEIERIEPSEEVSFSDAEKSLARILQQWMGITELVPLGDKWKGGIMELKPSNPSL
                     KSKEMPIESFFHKIVMLRDRLRVMEQRINSHDAFSDEDKVNMQQYITRIYGSLTSFNV
                     LFKYPEHQFVGEKSS"
     gene            complement(3796..4374)
                     /locus_tag="PKV31_06320"
     CDS             complement(3796..4374)
                     /locus_tag="PKV31_06320"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNB92505.1"
                     /translation="MDFLKKHTLFLASGSPRRADLMRQAGFLFTVINNDVDESYPENL
                     DVTKVAEYLSLVKAEAAKEFIVDDNSVVIAADSVVIIDGEIIGKPVDRADAQRILRKL
                     SGRAHVVITGVTLLGKLKKVTFSESATVHFYQLTDDEIDFYLSNFSPMDKAGAYGIQD
                     WIGVCRVRSITGNFSNIMGLPVSRLYNELKDF"
     gene            complement(4437..5366)
                     /locus_tag="PKV31_06325"
     CDS             complement(4437..5366)
                     /locus_tag="PKV31_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNB92506.1"
                     /translation="MTSLRDILKLIRPINALIVFATLMVVRLILFQYYNPFKIQPSID
                     AADYFLMMVTGMLILGAGNVINDIIDVDIDRLNKPRKALIPRRVSIRLAWVVYFSLNI
                     LALIFSVYLILKYQVGWYIFMPFCAILLLYVYSRWLKKKFFIGNLVIALLCGVLGLVG
                     FWVEMDNIHTIKELSDKDYTMILYSAGSIFIFSFLLVLARELIKDCEDMVGDRSAGAT
                     TIPIKLGLVRSDRLIMFLWVLYGVVFLALSIARYKLYESTPMMIAIIIYLIIYVLSIR
                     WIMVRFADKNLYSRLSTLVKIFLILGLIFILIK"
     gene            complement(5430..5957)
                     /locus_tag="PKV31_06330"
     CDS             complement(5430..5957)
                     /locus_tag="PKV31_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNB92507.1"
                     /translation="MDLFDKMRAVKAFVFDVDGVMTDSRVHVWENGDQTRTMNTRDGF
                     ALKRAILKGYRVAVITGGKSKGVTSRLNGLGVLDVYSGIADKLIALEDFRKRYDVSYD
                     QIMYMGDDLIDASVMKRVGLPAAPRDAVPAILNISSFVSSLDGGDGCVREIIEKIMIL
                     QGTWDWEVGNEQPGI"
     gene            complement(5961..6203)
                     /gene="iscX"
                     /locus_tag="PKV31_06335"
     CDS             complement(5961..6203)
                     /gene="iscX"
                     /locus_tag="PKV31_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNB92508.1"
                     /translation="MIKDLENIPIHWADYEDIAMSLYEKFGDEFGESSIYRIRFTDLI
                     EWVLDLPNFVGTREECNEGHLEQIQTHWVYEWRDNQ"
     gene            complement(6228..>6430)
                     /locus_tag="PKV31_06340"
     CDS             complement(6228..>6430)
                     /locus_tag="PKV31_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB92509.1"
                     /translation="CSTCHVYVYEGEDDLEEISDKEEDFIDRAINPKLNSRLGCQCII
                     LSDDAEIVVEIPDQSNIIGHEH"
BASE COUNT         1999 a         1461 c         1132 g         1838 t
ORIGIN      
        1 tatcgcaatc atactggcta ttttctttat ctatggtgtt tcacgatatt tcctgatcaa
       61 tcattggcgt tcgtatactg acgccatttt ttacccaact aaagaggaaa tcaaaataaa
      121 ggaaagcact ttgagattaa cagaaaaact ggacttgaat atggccagtt cacaccaatt
      181 ggtacaaata ggcttgccgc aaaatatatc aaatcgcatt agccgttaca gagataaaat
      241 cggaggcttc aaggattggc aacaagttga acggacctat ggaataacca aagagcaact
      301 ggatatactg catcaatact gctctatcag attagcttca aaaaagaggc ataataccca
      361 aacatcttat acccccaacc attttcataa aaagaaaaaa ggagtccata taccttcttt
      421 acttcctttt gatcccaaca ccgtggaact aaaggatctg gaagccatgg gcataagacc
      481 ttccatagcc aggggaattg tcaatttccg taagtccgga tttatatatc gtaataaaga
      541 ggacttgtta aagatatatg caatggatga ggtgacattt gaaaagctaa aaccattcat
      601 caaactggag aaacagaaag ttgaaaccca aactttaaaa aagagaagtg aaacaatcat
      661 tgacccggta gatatcaata gggcgaccgt ggaaagcctt agcaggttgc ccggtattgg
      721 aaaatattat tcacggctga ttctaaactg gcgagataaa ttgggtggtt ttcaccggat
      781 tgagcaaatt gcatccacat atcatcttcc ggatagtata ttcagcaaaa tagcggtata
      841 tatcaccttt gatacgccgc ccaatcggat cgacatcaac aaagcagatt cggaaacact
      901 agcaaagcat ttttttatca gggctaaaga agcaaaaatt attgtcgctt atcgtgataa
      961 tcatggtcca tatcaaactg tagaagatct attgaagacc ggatccattg acagagactg
     1021 gctcgaaaag gcaggcccgt accttattgc cggtaaataa ttcctgctta ctttcggcct
     1081 gtcttgtcaa aaagcattga actggcttgt tgggtagagg taacaagtac gtcctgtata
     1141 ttgacatgtg gtggccggga gagaatgaat gcaatgatct ctgcaacatc cttagccttg
     1201 agtggtgtat aatcgtcata aattcttgct ctctgagcat ctccatcaaa gcgcacaagg
     1261 gcgaattcag tctcttccac agcaccggga ctgactgagg aaacacggat tccgtatttg
     1321 tacaaatcca ttcgcattgc tcgggttagt gcttctacag cgaatttggt agcgttatat
     1381 acattaccat tgggataggt ctccttgcct gctgtagagc cgagattgat gatgtggcct
     1441 tgcctacgct cgaccattcc gggagaaatg agtctggtca tatggaggag acctttaata
     1501 ttggtatcga tcatttgttc ccaatggtca aacgatcctt cgtgaatttc actcaaacct
     1561 ttagccagac ctgcattgtt gataaggata tcgatctgct tcaaaccatc cggaaggcca
     1621 tcccaaaaaa ctttgcatgc gtttaaatcc cggatatcaa aagcaaaaac atgaacttcg
     1681 gttttgtccg accgaatgag ctcttcctta agcgcttcca acctctcttt ccttcttccg
     1741 gttaatatca acctatagcg tcccatggca cttaattcac gtgccgtggc cttgccgatg
     1801 cctgaagtcg ctccggtcag gagtattgtc ttcaccattt tgttaatttt tctacctgaa
     1861 gatcaaaatc ttttttaaaa gcttcgtatt catctgttgc gggaccgctg aatccggtat
     1921 gcacactgac aacatgattg ttcctgtcca gaaaaatcat cgtcggataa gcctttatcc
     1981 cactgataaa tggcactcgt gaagaggctt caggccccgg ctttcccgcc cacaaaactg
     2041 tatagggcaa atcaagtacc tttttataat tagacaacaa cgatctgacc tttgcggtat
     2101 catcaaatgc ttcaaatgac aaagctataa ctgaaagcct atcgttttga cgcttattca
     2161 aatattcagt taaaaatctg gcttcatcct tacaattggg gcaccaggtt ccggttattg
     2221 taagaacttt tgcctttcca ttgaaagttg gatcagaaag actcaccaaa ttgccttgct
     2281 catcaggaaa agtaaagttt acagcctgat ttccggaggc cctggtaagg atggatgcat
     2341 caggcaagat tgcatcagga ttaaaaacag cttcccatgt agctctgctc gtcaaccctg
     2401 acataaatgt acccttcatc ctgccttttt ctgttatttt accgctgacc aaataagctg
     2461 ctccaccggc gaatccgctc aaaaacattc tatccccttg tacggtacct tccaggaagc
     2521 catagtctcc ggtaggagaa gcaaatgttc ctttaaggct gttattatct tgtgtaaatt
     2581 gaccgatggt agtatcattc ctgttttcaa agtgaaaaat aacatcccac ttgcccgaaa
     2641 tatcaaatga aggcttttcg tttaccattg taaaccgata atcttttccg gcaagtcctc
     2701 tgaacgggac tctccagttt ttccgggggt tataccatgc gcctgtcatg atgccatcct
     2761 ccacttcagc ccttaaataa gtatcataag caacaaaatt gatccgtatt gtgtcctttc
     2821 cacttcttct gttgaaaccc cataatgtct cttctgccct cacccgatta tctccattga
     2881 taaaatcgac aaacatgctg tcaccctcgt aggtgacctc gaaattaaat ggcagtactc
     2941 cctctgtaaa ggcattctta aaagagatat cctgatcatt accaactttg gaaatatttc
     3001 ttgagagggg attgctttca tccagatata attcaccccg ccaaataccg ggcattagtt
     3061 tgtcaaattt ctgattggtt accaaacagg aattgaaaat ataaataact aacaaaggaa
     3121 gtaagacaac tttccaccac ttggataaca tataattctt ttacaagtaa accaacaaaa
     3181 tttaaatcag tttatcccga atcaactgct tttttcacca acgaactgat gttccggata
     3241 tttaaacaag acattgaagg aagtcaatga cccatagatc ctggtaatat attgttgcat
     3301 attgacttta tcttcatcac taaaggcatc atgactgttg atacgctgct ccataacacg
     3361 tagacggtcc ctaagcataa ctattttgtg aaaaaaagac tctatcggca tttcctttga
     3421 cttcaaagaa ggattcgaag gcttcagctc catgattcct cccttccatt tatctcctaa
     3481 aggcaccaat tcagttatgc ccatccattg ttgaaggatt cgtgccaggg atttttcggc
     3541 atctgaaaaa ctaacctctt cacttggttc gattctttca atctcagaaa accggtcata
     3601 atccttactg atttgcttga tgccatatgt catgaaacaa atttcataat tttcaggatt
     3661 cagtttaata actactccat caccatagga cggatgactt agtcgacttc caactccata
     3721 taactccata gatttcaaat tttaagccgt aaaatagcaa tatctttaca aacaggtgta
     3781 aaaattggtg atagatcaaa aatccttcaa ttcattatac agcctactca ccggaagacc
     3841 cataatattg ctgaaattgc ctgtaataga tctaaccctg caaactccaa tccaatcctg
     3901 gataccatag gcacctgcct tatccatcgg agaaaaattc gataaataaa aatcaatctc
     3961 atcgtctgtc agttgataaa aatggactgt agccgattcg ctgaaagtca ccttctttaa
     4021 tttgccgagt aaagtaactc cggtaatcac gacatgtgcc cgaccggaca attttcttaa
     4081 tatcctttgt gcatcagccc gatcaacagg cttacctatt atttccccat cgataataac
     4141 gacactgtcg gctgcaatta ccaccgaatt gtcatcaaca atgaattctt ttgcagcctc
     4201 tgcttttaca agggataaat attcagcaac tttagtgaca tcaagattct cgggataaga
     4261 ctcatcaaca tcattattaa tcacagtaaa taagaaaccg gcctgccgca ttaaatcagc
     4321 tctacgtggg ctcccggagg ccaggaaaag tgtgtgtttc tttagaaaat ccatgatact
     4381 tctaaaaaga gttgaaattt atatattatt ttgtataata tttattttac aaataatcat
     4441 tttattaata taaaaattag tcctaaaatc aaaaatatct tgacaagcgt actcagtcgg
     4501 gagtataaat tcttgtctgc aaatctaacc atgatccatc tgatgcttaa aacataaatt
     4561 atcaaataaa tgataatagc tatcatcatc ggagtggatt catacagctt atatctggcg
     4621 atggataaag ccagaaaaac gacaccatat aaaacccaga gaaacatgat caaacgatca
     4681 gagcgtacaa gaccaagttt aatcggtatt gtagttgcgc cggcactccg atctccgacc
     4741 atgtcttcac aatcctttat caattcgcgc gcaagtacca gcaaaaaact gaaaataaag
     4801 atacttccgg ctgaatataa aatcatggta taatctttat ctgatagctc tttaatagtg
     4861 tgaatattgt ccatctctac ccaaaatcca accaaaccga gtactccaca gagaagagcg
     4921 atcactaaat tcccaataaa aaacttcttc tttagccaac gtgagtacac atacaacaac
     4981 aagattgcac aaaatggcat gaaaatatac cagccaacct gatatttcag gatcaaatag
     5041 actgaaaaaa tcaatgcaag gatattcaat gagaaatata ccacccatgc cagcctgatt
     5101 gaaacacgtc tcgggataag tgctttccgg ggcttattaa gtcggtcgat atcgacatcg
     5161 ataatatcgt tgattacatt accggctccc agaataagca taccggtgac catcatcagg
     5221 aaatagtccg ctgcatctat ggaaggctga attttaaaag gattataata ttggaaaaga
     5281 atcaacctca ccaccatcag tgtagcgaat acaatcaagg cattgatagg cctgatgagc
     5341 tttaaaatat cccgtagaga agtcacagcc taaaggtatg gattgtaggc taatccgcct
     5401 taatttttga ttggtgtttg ataaataaat tatatacctg gctgttcgtt gcctacctcc
     5461 caatcccagg tcccttgtaa tatcataatt ttctcaatga tctcccgtac gcatccatca
     5521 cccccatcca atgatgaaac aaatgaagat atattaagaa tggccggtac agcatcccgc
     5581 ggggctgcag gcagtccgac gcgtttcatc accgaagcat cgattagatc atcccccatg
     5641 tacatgattt gatcataact tacgtcatac cttttgcgaa aatcttccaa agcaatcaat
     5701 ttatcagcaa ttcctgaata aacgtcaaga acaccaagac cattgagcct ggatgtcaca
     5761 cccttgcttt tacctccggt aatgacggcc acacgatatc cttttaaaat ggcccttttt
     5821 aatgcaaatc catctctggt gttcattgtt cgtgtctgat cgccattctc ccaaacatgt
     5881 actctggaat cagtcattac tccgtcaaca tcaaagacga aggctttgac agctctcatt
     5941 ttgtcgaaca aatccatagc ctattgatta tctcgccact cgtacaccca gtgtgtctgg
     6001 atctgctcca gatgaccctc attgcattct tctctggtac cgacaaaatt gggtaaatca
     6061 agaacccatt caataagatc cgtgaaacga atcctatata tagagctttc tccaaattca
     6121 tctccgaact tttcgtaaag agacattgcg atatcttcat aatccgccca atggatcgga
     6181 atgttttcca aatctttaat catattctac tgaattaggc tgcaaagtta atgttcgtgt
     6241 ccaataatat tgctttgatc gggaatttcg acaacaatct ctgcatcgtc cgaaaggata
     6301 atgcactgac aacccaatcg gctgttgagt ttcggattga tagcccggtc tataaaatct
     6361 tcctctttgt cagaaatctc ttccagatca tcctctccct cataaacata cacgtggcaa
     6421 gtactgcatg
//
LOCUS       DAOQMC010000131         6415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5100_length_6415_cov_5.328302, whole genome shotgun sequence.
ACCESSION   DAOQMC010000131 DAOQMC010000000
VERSION     DAOQMC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5100_length_6415_cov_5.328302"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKV31_06345"
     CDS             <1..499
                     /locus_tag="PKV31_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB92510.1"
                     /translation="NAQGKYFTRSGTISFVSDALLEKNEAVNNKVSAAIDASTGKVEF
                     IVLIKSFQFKKALMQEHFNENYMESGKYPKSTFSGRITNLKDIDFTKDGAYKVMVTGV
                     LTMHGVTKDVATQGTINIVKGVANVQSKIRVKLADYNIQIPAIVKDKVAQEVDVMIDC
                     KLKKV"
     gene            563..1465
                     /locus_tag="PKV31_06350"
     CDS             563..1465
                     /locus_tag="PKV31_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNB92511.1"
                     /translation="MRNKLYASLLVVALLISVKSYSQSDSSLIAMLDGSAQDVDYTYA
                     TFKTNRIINLHSIENTAAGVLDVKISHRFGLINQGLYDIFGLDAAQMHLNFDLGLTDR
                     LAIGVGRTSLDKAYNGFVKYKILKQSTGSKNMPVSVSFAAGTVIKTSRSLDPDPGNTF
                     WYRVYYDFGLLIARKFSENFSLQVTPTLVHRNLVRTAEEKNDVFAIGVGARQKLTRRM
                     SINAEYIYVLPGQLAPDKLNSLSVGLDIETGGHVFQLHFTNSQGMNENAFITETINRW
                     DKFEFRFGFNLSRVFTLWEPAIRD"
     gene            complement(1639..2352)
                     /locus_tag="PKV31_06355"
     CDS             complement(1639..2352)
                     /locus_tag="PKV31_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB92512.1"
                     /translation="MFSTFLFTMFLFLSGFCLCQQKTIEPDCGLKVSYYNLSHRVAFQ
                     PRKLIVPAGLIVTGTALNFKPIKSVKSNIVSVRNEYLPGFRIYVDDYLQFAPIVAAYG
                     MDLLGVPSKNDFINRSVILLKGELAMAGITTLIKYTTQYNRPDHSNRHSFPSGHTAQA
                     FVAATFLSEEYKDRLPWIPYISYGVASSVGILRMANNKHYLSDVLAGAGIGILTMKVA
                     YWTHKYKWNNKKKEKYSEF"
     gene            complement(2410..2937)
                     /locus_tag="PKV31_06360"
     CDS             complement(2410..2937)
                     /locus_tag="PKV31_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92513.1"
                     /translation="MDRAELDAQSVFYEKQSFRQWWIWLILLGVDGIFVVGAILQLFM
                     GRQFGDNPMPDFVLILVCGVILFSSFLFYSIHLETRICWDGIYVRFFPFHFKYKIFYW
                     DTIKKCYVREYAPVKEYGGWGIRNSLNGRKKAYNISGNQGIQLEFTDGKNLLIGTQKV
                     DEVINTLKYLKRWKA"
     gene            complement(3096..4142)
                     /locus_tag="PKV31_06365"
     CDS             complement(3096..4142)
                     /locus_tag="PKV31_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92514.1"
                     /translation="MNTKHVLVTGASGTVGREVVKILADQSDFRITVFDIANQQSEPF
                     FNSFGERIQFIPGNISDVADVSRIPEDLDAVIHLAAIIPPLADEDPGLARKVNVEGTG
                     LLIDHIERTSPGAFFLYSSSVSVYGDRVENPDIRVTDDLKISEGDEYGQTKLEAESII
                     QSSNLSWSVFRLAAIMGNHKISRLMFHMPLETSLEICTPEDTARAFVHALSAQDRLEY
                     RIFNLGGGKQCRSSYREFLERSFNIFGLGRLDFPKQAFALHNYHCGNMADGDELEEIL
                     HFRRDTMDSYFRKTANSVSPLKRWFTMLFRPIIKWFLLRQSEPYRAYRKGDVRMMSQF
                     FPLLPEFNLKAVER"
     gene            complement(4242..5321)
                     /locus_tag="PKV31_06370"
     CDS             complement(4242..5321)
                     /locus_tag="PKV31_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB92515.1"
                     /translation="MLTLAWIGVSQGFFAAILMVAKQKNSVSDKILSAWLFLFGLDFL
                     SCALDYMIFGKPLLTSSFLLFNPALYLYVKSLVLPRFRLRWTQLLHLLPYIIFKVLAY
                     VYREPFTMEGYFERDGHLVYRLIFGASTIVSWLVYNSLTLKYVHLYRVKLFHEQSNIE
                     KSNNLTWVLTVAVFYVSYCIIAFMMAVAAYIGWFGPLSPHIYNYFVLLFAIYILSFYG
                     LYQQELPEHLLIEESVPVAYKNSSLSPEAKEDIRKRIEKFMIGQKPYLNPGLNMDMLS
                     EALNIPKYQLTEVLNTVIGSNFFQYVNKFRVEAVKDMLLDPTNHFSIEAIGFECGFAS
                     KSSFYSVFKNMTGKTPIAFRESVVS"
     gene            complement(5510..5929)
                     /locus_tag="PKV31_06375"
     CDS             complement(5510..5929)
                     /locus_tag="PKV31_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB92516.1"
                     /translation="MNTNKLVSEVMTSNVMTADRRDSLRHVNSLMKEKHIRHLPVVDN
                     GKLVGIVSNTDIMRLSFGDLYEGETGADEAMFDMLTLDQVMVHRPVSVRHDEFVEDVA
                     LKLASAEYHAFPVIDGDKVVGIITTTDVIKYLLNRGE"
BASE COUNT         2018 a         1350 c         1279 g         1768 t
ORIGIN      
        1 gaatgctcaa ggtaaatatt ttactcgctc agggacgatc agttttgtct cagatgcctt
       61 gctggaaaaa aatgaagcgg tcaacaacaa agtaagtgca gccattgacg cctcaaccgg
      121 aaaggtggaa ttcatcgtat tgatcaagtc cttccagttt aaaaaagcat tgatgcagga
      181 gcatttcaac gaaaattaca tggaatccgg caaatatccc aaatccacat tttccggccg
      241 gatcaccaac cttaaagata tcgacttcac aaaagatgga gcttataagg tgatggttac
      301 aggtgttctg accatgcatg gagtaaccaa ggatgtagcc acccagggta caatcaatat
      361 cgtcaaagga gttgccaatg tgcaaagtaa gattcgggtg aagctggctg attacaacat
      421 acagattccg gctatcgtca aggataaggt ggcacaggaa gtggatgtaa tgatcgattg
      481 taagttgaaa aaagtttaat gacctgcgaa gcttaggatt ctgcgcctga tttcaatatt
      541 ttttcatcat taaaaatctc taatgcggaa caaactatac gctagtctat tggttgtcgc
      601 tctcctcatt tcagtcaaat cttatagcca atccgacagc agtcttattg caatgctcga
      661 tggttcggca caggatgtcg attatacgta tgctaccttt aagaccaacc gaattatcaa
      721 ccttcattcc atagaaaaca cagctgccgg tgtgttggat gtgaagataa gccatcggtt
      781 tgggctcatc aaccaggggc tttatgatat atttggattg gatgccgctc agatgcactt
      841 gaattttgat cttggcctga cagacaggct ggcaatcggt gttggtcgta catccctgga
      901 caaggcttat aatgggtttg tgaaatacaa aatattaaag caaagcacgg gcagtaaaaa
      961 tatgcctgtt tccgtttcat ttgctgcagg aacagtaatc aaaacctcac gatcgcttga
     1021 tcctgatccc ggcaacacat tctggtacag ggtttattat gattttgggc tgttaattgc
     1081 gaggaaattt tcagagaatt ttagtttgca ggtaacaccg acccttgtcc atcggaatct
     1141 cgtacgtacc gcagaagaaa agaatgatgt atttgccata ggcgtgggtg ccagacaaaa
     1201 actgacgcgg aggatgagta tcaacgcaga atatatctat gttttaccgg gtcagcttgc
     1261 tcctgacaag ttaaattcgc tttctgtagg gcttgatatc gagaccggcg ggcatgtatt
     1321 tcagctgcat tttaccaaca gccaagggat gaatgaaaat gcatttatca cagagaccat
     1381 aaacaggtgg gacaagtttg aattccggtt cggcttcaac ctttcacgcg ttttcacgct
     1441 ttgggagccc gcgattcggg attgattacc gggaaatact actcatgaaa ttatctgagg
     1501 atcatcatcg tcccggtgat gttgtgagtt ccgcttgtgg cacttgctaa ggtcagcatc
     1561 tttactaaac agatcacatt ttgcccaagc ccctcgttca tttttgaaat agataacttt
     1621 ccttcaggga caatcctgtc aaaactccga atatttttct ttcttcttgt tattccactt
     1681 atatttatgt gtccagtagg caactttcat tgtcaggatg ccaataccgg ccccggcaag
     1741 tacatcactg agataatgct tgttgttggc cattcggagg atgccaactg aagatgctac
     1801 accgtagctg atatacggaa tccatggcag gcggtcctta tactcttcac taaggaaggt
     1861 ggcagcgaca aatgcttgag cagtatgtcc cgaaggaaat gaatgtctgt tggagtggtc
     1921 ggggcggtta tattgggttg tatatttgat cagtgttgtg ataccggcca ttgccagttc
     1981 gcctttcaaa agaataacgc ttcggttgat aaagtcattc tttgatggca ctcccaaaag
     2041 gtccatgcca tatgctgcca caataggagc aaactgaaga tagtcgtcca cataaatgcg
     2101 aaaacccggc agatattcgt tgcgtacact tacgatattg ctcttaacgc ttttgatagg
     2161 cttaaaattg agggcagtcc cggtaacgat caaaccagca gggacaatca atttacgcgg
     2221 ctgaaaggca acccggtgag ataggttata ataggatacc ttaagaccac aatccggctc
     2281 aatcgttttt tgctgacaga ggcagaaacc ggaaagaaaa agaaacatag taaacagaaa
     2341 agtcgagaac attccggaat attttaggca aaaatatact ttttttaaaa gaccagtcat
     2401 agcgccaatt tatgctttcc aacgcttaag atatttgagc gtatttatga cttcatcaac
     2461 cttctgagtt cctatgagca aatttttacc atctgtaaac tccagttgaa ttccttgatt
     2521 accggagatg ttgtaggctt ttttccgtcc gttcaaactg tttcggatcc cccatcctcc
     2581 atattctttg acaggcgcat attctcgaac ataacacttc ttgatggtat cccaatagaa
     2641 aatcttgtat ttaaaatgga atggaaaaaa tctcacatag attccatccc agcaaatcct
     2701 cgtctccaaa tggatggaat aaaaaagaaa actcgaaaat aatataacgc cacacacaag
     2761 aatcaataca aaatcaggca tagggttgtc accaaactgc ctgcccatga ataattggag
     2821 aatcgcacca acgacaaaaa taccatcgac acccagaaga atcaaccaaa tccaccattg
     2881 ccggaaactt tgtttttcgt aaaaaacaga ttgtgcatcc aattccgctc tatccattat
     2941 caaataattt aatttcaaat ttactgcaaa aaacgcaata aaacgccctc aatcaaagaa
     3001 taaatagtat aaaaaatccg gattccatta aattaatgaa atccggagaa taaacttaaa
     3061 aaaactaaaa aacaaaatcc tgttcgggtg atgatttacc tttcaacagc ctttagatta
     3121 aattccggca gcaaaggaaa gaactgggac atcatcctga catcaccctt tcgataagcc
     3181 cgataaggct ctgactgacg aagaaggaac catttaatga tcggccggaa tagcatggta
     3241 aaccatcttt tcaagggaga aactgaattt gctgtcttcc ggaaataaga atccattgta
     3301 tcccgtcgaa aatgaaggat ttcttccagc tcgtcaccat ctgccatatt gccacaatga
     3361 tagttgtgaa gcgcaaaagc ctgttttgga aaatccagtc gacccaatcc gaaaatattg
     3421 aaggaacgct ccaaaaattc acgataagac gatctgcatt gttttccgcc tccgagattg
     3481 aaaatccggt attctaatcg atcctgtgcg gataaggcat gtacgaatgc tctggccgta
     3541 tcttcaggtg tacatatctc caggcttgtt tccagaggca tatgaaacat cagtcgagaa
     3601 atcttatgat tgcccataat agctgccaat cgaaatactg accaactaag attacttgat
     3661 tggataatag attccgcttc cagtttggtt tgaccatatt catccccttc cgatatcttc
     3721 aggtcatccg taactctgat atccggattt tcaacacggt caccatatac cgaaacagat
     3781 gaactatata gaaagaaagc tccgggtgat gttctttcta tatgatcaat caacaaaccg
     3841 gtcccttcga cattgacttt tcgtgcaagt ccgggatctt catctgccaa tggtggtatg
     3901 atggcagcca gatgtatgac agcatccaga tcttccggaa ttcttgacac atctgccaca
     3961 tcgcttatat tgccggggat gaactgaatg cgttcgccga atgagttgaa aaaaggctca
     4021 gattgttggt tcgctatatc aaagactgta atccgaaaat cactttggtc cgccaatatc
     4081 tttacaacct ctcttccaac cgtacctgat gcacctgtga ctaaaacatg ttttgtattc
     4141 atttgaaatc atttttgcaa tgataacgga gaaaattgag caaaatagtc caggttttaa
     4201 agattggact acatgtttat gtcctgattg caaaattcag actaggaaac cacagattcc
     4261 ctaaaggcaa tcggagtttt gccggtcatg tttttaaaaa cggaataaaa agaggattta
     4321 ctggcaaatc cgcattcaaa gccgatcgct tcaatggaaa aatgattggt cggatctaaa
     4381 agcatatcct tcacggcttc gaccctgaat ttgttgacat attgaaaaaa attgctccca
     4441 ataactgtat tcaatacttc agtcaattgg tatttaggta tattcaacgc ttcagaaagc
     4501 atatccatat tcagcccggg attaagatat ggcttttgcc cgatcataaa tttttcaatg
     4561 cgcttcctga tatcttcctt ggcttcaggg cttaacgaag agtttttata agccacgggc
     4621 accgactcct ctatgaggag atgctccggc aactcctgct ggtaaagacc gtaaaaactc
     4681 aggatgtaga ttgcaaataa tagaacaaaa tagttgtaaa tatgtgggct tagcggtcca
     4741 aaccagccta tataggcagc aacagccatc ataaaggcaa tgatgcaata actgacataa
     4801 aatacagcaa ccgtcaaaac ccaggttagg ttgttgcttt tttcaatgtt ggattgttca
     4861 tggaataatt tcacccgata cagatgtaca tacttgagtg taagggaatt atataccaac
     4921 caggaaacga tagttgacgc accgaatata agcctgtaaa ccaaatggcc atcccgttca
     4981 aaatagcctt ccatcgtaaa gggttcccgg taaacatacg ccaacacctt aaagatgatg
     5041 tagggcaaaa gatggagtaa ttgggtccat ctaagtctga accgaggcaa taccaaagat
     5101 ttgacgtaca gatacaaagc cggattgaac aggagaaagg agcttgtcag taaaggctta
     5161 ccaaaaatca tataatccag agcacaactt agaaagtcca gtccgaaaag aaataaccag
     5221 gcggataaaa tcttatccga tacggaattc ttctgtttag ccaccatgag gatagccgca
     5281 aaaaaaccct gactaacacc aatccaggca agtgtcaaca tgtacagtct attatagata
     5341 caaatataca ggatggaaga tgaacgcata ggatattatg cgttatcatc atagattagc
     5401 agatgatgtc aacatattgc tgcaaatcag aatcaaggat tccgacttca acaaatgaag
     5461 attatggatc caaccttcct gttattgaag caatcccttc cattcccaac tattcacccc
     5521 tgttgagcag gtatttgata acatccgtcg tggtgataat gcccaccacc ttgtccccat
     5581 ctataaccgg aaaagcatga tattcagcag aagcaagctt gagagccaca tcttcgacaa
     5641 attcatcatg acgaacactg accggcctat gaaccatcac ctgatcaaga gtgagcatat
     5701 cgaacatggc ctcatccgcc ccggtctctc cttcatacag atcaccgaaa ctcaggcgca
     5761 tgatgtcggt attgctgacg atgcctacca attttccatt gtcaactacc ggcaaatggc
     5821 gtatatgttt ctccttcatg agtgaattta catgacgcaa actatctctc ctgtcagcag
     5881 tcattacgtt gctggtcatt acttcggata ccaatttatt cgtattcatt tcaaaattat
     5941 tatgatgagt aaatttcgtg aagtaatcaa atacaattta tgattctgcg catagttttc
     6001 taaaaaaatg aagtcttttg aaaaaacctg aatgcgtgta cattaatcgg caaatcaaat
     6061 tatataactg actttgataa gagatgattc attggggaat cccggtcaga atgtgtttgg
     6121 gctaagctgt ataattcttt aagctcccgg gattgtaaag agacttgatc aaccaaaaag
     6181 tgtggctcat tgatggccct gagcctgcct gatagctcag attcagttag atgtgcggaa
     6241 gtatcgctgg ttgaccttgg gtatgtattg aaattaaata catgtcaagg tcaaataaga
     6301 aaatattacg gtgggacctt aaccaatatt tgatcaaact accgacaata atgtgtaaaa
     6361 aattatataa tatgtataat tatgtcaatc tgattattac aaattaaata ttttt
//
LOCUS       DAOQMC010000132         6392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5135_length_6392_cov_4.545053, whole genome shotgun sequence.
ACCESSION   DAOQMC010000132 DAOQMC010000000
VERSION     DAOQMC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5135_length_6392_cov_4.545053"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3052)
                     /locus_tag="PKV31_06380"
     CDS             complement(<1..3052)
                     /locus_tag="PKV31_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB92517.1"
                     /translation="MRRIMQASGAEIIHLGHNRSADEIVNAAIQEDAQAIAITSYQGG
                     HNEFFKYIYDLLKERHAGHIKVFGGGGGTILPEEIKQLEAYGIARIYSPDDGRALGLQ
                     GMIDDVLRLCDFPVGDQLNINTGQLNSHNPHVVARIISAAENYPDQHRELLGKLNEKA
                     EASAAPVLGITGTGGAGKSSLIDELIRRFLLEYKDKTMGIISVDPSKRKTGGALLGDR
                     IRMNSINKPRIYMRSMATRQSNQALSAQINAALNILKAAGFDLIILESSGIGQSDTEI
                     TQYADVSLYVMTPEFGAASQLEKIGMLDYADAVAINKFDKRGAADALRDVKKQYQRNH
                     QLFDQSPDKMPVFGTIASQFNDPGTNAFYLHVMKLINDRSKGRFTSQRALTAGPSEKI
                     HIIPPERTRYLSEISEECRRYDSWAETQSEIAGQMQALNESIKMLSKDETSKDKLREK
                     YEEVSRKLSRDNEELLDNWPAKKANYEGEYFSYVVRGKEIKVETTTESLSHLRIPKVS
                     LPKFHGWRDILYWSLKENVPGEFPFTAGVFPFKRKGEDPTRMFAGEGHPERTNRRFHY
                     VSLGMPANRLSTAFDSVTLYGEDPDYRPDIYGKIGNSGVSVCCLDDAKKLYSGFDLCD
                     PSTSVSMTINGPAATICAYFMNAAIDQQCEKYIVQHRLEHIVEEKLREKYDTRGLKRP
                     EYQGNIPEGNNGLGLLLLGVTGDEILPAEIYNEIKARALSSVRGTVQADILKEDQAQN
                     TCIFSTEFSLKLMGDVQEYFIQHDVNNFYSVSISGYHIAEAGANPISQLAFTLANGFT
                     YVEYYLSRGMDINDFAKNLSFFFSNGVDPEYAVIGRVARRIWAKAMKLKYGADERAQK
                     LKYHIQTSGRSLHAQEIAFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRR
                     AMAIQLVINHELGLAKNENPMQGSFIIEQLTDLVEEAVLMEFDRITERGGVLGAMETG
                     YQRGKIQDESLYYENLKHTGALPLIGVNTFLSDDGSPTILPREVI"
     gene            complement(3432..4088)
                     /locus_tag="PKV31_06385"
     CDS             complement(3432..4088)
                     /locus_tag="PKV31_06385"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB92518.1"
                     /translation="MNIKYIVISVVLFTLSGFTAFAQRGYHGPIQLSGVVVTQEKGQP
                     VRMPYVTIAVEGTSRGTYTNWDGFFSLVVEKGETIVFTYVGYKTIRYVVPDTLSTNRY
                     SIFQIMTFDNILLPETVIYPWPDKDNYHAEFLAMNINNDITDRVQENLDRRVLASLYD
                     KVPVDGKESTQSFMRQNASTYYYQGQTRPIQLMNVMAWKQFIEAWKAGKFKKKQDDGK
                     "
     gene            complement(4507..5796)
                     /gene="eno"
                     /locus_tag="PKV31_06390"
     CDS             complement(4507..5796)
                     /gene="eno"
                     /locus_tag="PKV31_06390"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNB92519.1"
                     /translation="MSAIIDIRSREILDSRGNPTVEVEVLTEDGAVGRAAVPSGASTG
                     KHEAVELRDNDNKRFLGKGVLKACKHVDDEIRDEVVGMDVFLQREIDQVMIEADGTKN
                     KSKFGANALLGVSMAVAKAAAKEAGQPLYRYIGGVNACTLPVPMMNILNGGSHADNSI
                     DFQEFMIMPVGAPFFSESLRMGVEIFHHLKNVLKSKGYSTNVGDEGGFAPNIKSNEEA
                     IETVLMAIEKAGYRPGEDVLIALDAASTEFYNEKEKIYHFKKSDGRKLDSDEMVDFWK
                     KWVKKYPICSIEDGLAEDDWKGWSKLTDSIGKKVQIVGDDLFVTNTERLKKGIKEEAA
                     NAILVKVNQIGTLTETIEAVQLATRHGFNSVMSHRSGETEDTTIADLAVALNTGQIKT
                     GSASRSDRIAKYNQLLRIEEELLESGIFPGLNLFKRK"
     gene            complement(5866..>6392)
                     /locus_tag="PKV31_06395"
     CDS             complement(5866..>6392)
                     /locus_tag="PKV31_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767148.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNB92520.1"
                     /translation="LKVFPARTELSTIDAWEPDGYFLSNGPGDPAAMDYAIKSARKIL
                     DSNKPVFGICLGHQLLALAQGMKTEKLKYGHRGTNHPVLNTSTGLGEITSQNHGFGVV
                     KDSLIGMETEVEVSHYNLNDGSLEGLRWKNKRIFTVQYHPESSPGPFDSRYLFDEYIA
                     MLKVQRGIPVQVNA"
BASE COUNT         1608 a         1572 c         1321 g         1891 t
ORIGIN      
        1 tgatgacttc gcgtggcaat atcgttggcg atccgtcatc tgaaagaaag gtattcacac
       61 ctatcaatgg caatgctccg gtatgcttca gattctcgta atacaagctt tcatcctgaa
      121 ttttaccccg ctgatatccg gtctccattg ctcccaggac acctccgcgc tctgttatcc
      181 ggtcaaattc catcaatacc gcctcttcca ccaggtcggt caattgctca atgatgaacg
      241 agccctgcat cgggttttca ttcttggcaa gacccagctc atgattaatg actagctgaa
      301 tggccatggc acgcctcacc gattcttcag tcggagtggt tatggcctcg tcataggcat
      361 tggtatgtag cgaattgcag ttgtcataga tcgcatatag tgcctgtaga gtcgtcctaa
      421 tgtcgttgaa tgcaatttct tgtgcatgca atgacctgcc tgatgtctgg atatggtatt
      481 tcagcttttg agcccgttcg tcagctccat atttgagctt cattgctttt gcccagattc
      541 ggcgtgcgac acgtccgatg acggcatatt ccggatctac accattactg aaaaagaacg
      601 aaagattctt cgcaaaatcg ttgatatcca tgcctcgtga caaataatat tccacataag
      661 taaagccatt ggctaatgtg aaggccaact gagaaatagg attagctccg gcttcagcga
      721 tgtggtatcc ggatatggat acagaataga aattattgac atcatgctgg ataaaatatt
      781 cctgcacgtc gcccatcagc ttcaacgaaa actccgtaga gaatatacaa gtgttctgag
      841 cctgatcttc tttcaggatg tcagcctgaa cagttccgcg tacagaacta agtgcacggg
      901 ccttgatctc attatagatt tccgccggaa gaatctcatc gccggtgaca cccagaagca
      961 gcagacccaa accattgtta ccctccggta tgttaccttg atattcggga cgctttaggc
     1021 ccctggtatc atacttttcc ctaagctttt cctcaacgat gtgctccagc cggtgttgga
     1081 caatgtattt ttcacattgc tgatcaatgg cggcattcat gaaatatgca cagatagtcg
     1141 ctgccggacc attgatcgtc atactcactg acgtggatgg atcacaaaga tcaaatccgg
     1201 aatatagctt tttggcatcg tccaggcagc ataccgaaac accggaattc ccgatcttgc
     1261 catagatgtc cggacgataa tcaggatcct cgccgtataa agttaccgaa tcgaatgctg
     1321 ttgaaagcct gttggcaggc attccgaggc tcacatagtg aaatctcctg ttggtacgtt
     1381 ccggatggcc ctccccggca aacatacgtg tggggtcttc tccctttctt ttaaatggaa
     1441 aaacgcctgc tgtaaatgga aactcaccgg ggacattttc cttcaggctc caatatagaa
     1501 tatcccgcca tccgtgaaac ttaggcaggc ttactttagg aatccgaaga tgagacaagg
     1561 actcagtcgt tgtttcgacc ttgatctcct ttcctcgcac gacatatgag aagtattctc
     1621 cctcgtaatt tgcttttttg gcaggccagt tgtccaacaa ttcctcattg tctcgtgaaa
     1681 gcttcctgct cacttcctcg tacttttcac ggagtttgtc cttagaagtt tcatctttcg
     1741 acaacatttt gatagattca ttcagggcct gcatctgccc cgcgatttcg gattgtgttt
     1801 cggcccagga atcatagcga cgacattcct ccgatatctc agacaggtat ctggttcttt
     1861 caggcggtat gatgtgtatc ttctcggatg gtccagctgt aagagctctt tgactggtaa
     1921 atctgccttt ggatctatca ttgatcaatt tcatgacatg caggtaaaag gcattggtcc
     1981 ccgggtcgtt gaattgagaa gcgatcgtac caaatacagg catcttgtca ggggactggt
     2041 cgaaaagctg gtgatttcgc tggtattgct ttttgacatc gcgtaaagca tcggctgccc
     2101 cgcgcttgtc aaacttattg atagctacgg catcagcata gtccaacatc cctattttct
     2161 ccagctggct agccgcaccg aattccggag tcatgacgta aagcgaaaca tcagcatatt
     2221 gggtgatctc cgtatcagac tgaccgatac cgcttgattc aaggatgatg agatcaaatc
     2281 cggcagcctt gagtatattc agggcagcat taatctgtgc gctcaaggcc tggttggatt
     2341 ggcgggtcgc cattgacctc atgtatattc ggggcttatt gatcgaattc atccttatcc
     2401 ggtcgcctaa taaagctccg cctgtttttc ttttgctggg atcgacagag atgatgccca
     2461 tcgtcttatc cttatattct agtaagaaac ggcggatcag ttcgtcgata aggcttgact
     2521 tgcctgcacc accggtacct gttattccca ataccggagc tgccgatgcc tctgccttct
     2581 cattaagctt gccaagcaat tcccggtgct gatccggata attctcagcg gcagatatga
     2641 tcctggcgac gacatgtgga ttatggctgt tgagttggcc ggtattgata ttcaactggt
     2701 ctccaaccgg aaaatcacag agacgcaaca catcgtcgat catgccctga agccccaatg
     2761 cccgaccatc gtccggtgaa taaattctcg caataccata agcttccagt tgcttgattt
     2821 cttccggcag aatggtacct cctcctccgc caaaaacctt gatatgtccg gcatgccgct
     2881 ctttaagcag gtcatatata tacttaaaaa actcgttgtg acctccctga taggaagtga
     2941 tggctattgc ctgagcatct tcctgaattg cagcattgac aatttcgtcc gctgacctgt
     3001 tgtgacccag atgtataatc tctgcgccgg aagcctgcat gattcgcctc atgatatgat
     3061 ggctgcatcg tgaccatcga agagggagcc tgaagtaacg attctgatat gattggttgg
     3121 acggtaagga gtggtttctt tagccatatg ttaatgcttt acatatgccg gtagcactca
     3181 atttaaggaa ggggctccgg ctctatttcc gcacaaagat agtaaaattg cccgtgcacg
     3241 gcaagtacgc cctcaaaata attggacact ccatttccgt attgtacagg attctgtggt
     3301 tggttttctc atgtagatca ttaccaccga ctcccatctg cacaataata atgggagttt
     3361 aatccggcgc caatgcacat cagatagcct atatttctat tactccctac tccccaacac
     3421 cgggcaataa gctacttgcc gtcgtcctgc ttctttttga atttacctgc tttccaggct
     3481 tcaatgaatt gcttccaggc cattacattc atcagttgga tcggccgggt ctggccctga
     3541 tagtaataag tagaggcgtt ctgacgcata aatgactggg ttgattcttt tccgtcaacg
     3601 ggaactttat catataatga agccaggact cttcggtcga gattctcctg aaccctgtct
     3661 gttatatcgt tattaatgtt cattgccaga aattccgcat ggtagttgtc cttgtcaggc
     3721 catggataaa taactgtttc cggtaaaaga atattgtcaa aagtcatgat ttgaaagatg
     3781 gagtaacgat tggtgctcaa tgtatccggt acgacgtatc ggattgtttt gtaacccaca
     3841 taggtaaaca caatcgtttc ccccttttcc actaccaggg agaagaaacc gtcccagttg
     3901 gtatacgtac cccgactcgt accttcaact gcaatagtca cataaggcat cctgacaggc
     3961 tgtccctttt cctgagtgac cacaacacca ctcaattgga tgggtccgtg atacccacgt
     4021 tgtgcaaaag ctgtaaatcc tgagagggta aaaagaacta ctgagatgac aatatactta
     4081 atattcatgt ccttgttttg atgaaggccg gcttgataca agtccgtaaa tttatttcat
     4141 tataacgtac aaaagtaaaa aatgatgccg actccggcaa aagtggaaaa tatttcatgc
     4201 tttatccttt gacctacttt agtacgtttt aatttataga caaaaatgaa ttttcggtca
     4261 acagcaaata tcttatattt gtaaatattt gaattcctat actttgtatt taaatcgaaa
     4321 tatcttcaac tgaaaaaatc ctaagtttat tgatggatgg aaataaaatt aaaaagccgg
     4381 cggaattctc ctgccggcca ttttttaaca cctttttaat tattccaaaa gtcgctgatt
     4441 tttcgctttg tacctttttc ttgtctgtcg actctaataa aagtaatgaa ataatgagcc
     4501 tgcactctac tttcttttaa agaggttgag acccgggaag attccacttt caagcaattc
     4561 ttcctcgatc ctgagtaatt ggttgtattt ggcgatcctg tcagagcgag aagcagagcc
     4621 ggtcttaatc tggcctgtat tcaaagccac tgcaagatca gctatggttg tgtcttctgt
     4681 ctcacctgac cggtggctca tgactgagtt aaagccatgt ctagtagcca actgaaccgc
     4741 ttcaatggtt tccgtaaggg ttccgatttg atttaccttg acgagaatag cgtttgctgc
     4801 ttcttccttg atgcccttct ttagcctctc tgtattagtg acaaaaaggt catcacccac
     4861 tatctgtact ttcttcccta tactatcagt cagtttggac caacccttcc agtcgtcctc
     4921 tgccaatcca tcctcaatag aacatatcgg gtatttctta acccacttct tccaaaagtc
     4981 aaccatttcg tcagagtcga gctttctgcc atctgatttc ttaaaatggt aaattttctc
     5041 cttttcgttg taaaattcgg ttgatgcggc atccaaagcg atcagtacat cctctcccgg
     5101 tctgtaaccg gctttttcga tagccatcaa cacagtctct atcgcttcct cattggattt
     5161 gatattaggt gcaaaaccac cttcatcacc gacattggta gaatacccct tggatttgag
     5221 cacatttttc aggtgatgaa atatctccac acccatacgc aaagactctg agaaaaacgg
     5281 cgcccccacc ggcataatca tgaattcctg gaagtcgatg ctattatcgg catgactgcc
     5341 tccattcagg atgttcatca ttggaacagg cagggtgcag gcgttgacgc caccgatata
     5401 cctgtacaaa ggctggccgg cttcttttgc agccgctttt gctaccgcca ttgaaactcc
     5461 caaaagtgca ttggcaccaa attttgattt attcttggta ccatctgcct ctatcatgac
     5521 ctgatcaatc tcacgttgca ggaagacatc catccctacc acttcatccc tgatttcgtc
     5581 gtcaacatgc ttgcatgcct tcagtacgcc cttcccgagg aatcgcttat tatcattgtc
     5641 tctcaattcg accgcttcat gttttccggt agatgctcct gaaggcactg ccgcacggcc
     5701 aactgccccg tcttccgtga ggacttcgac ttcgaccgtt ggattccccc ggctatccag
     5761 tatttctctt gatctgatat ctatgattgc gctcatttcc taaaattaat agtttttgga
     5821 gataaatata ttaaattttt ttcaatatca atgtttcttt caattttaag cattgacttg
     5881 cacgggtatt cccctttgca ccttcagcat ggctatatat tcatcgaaaa ggtaccgact
     5941 atcaaaaggt cccggactag actccggatg gtactgcacg gtaaatatcc ttttattttt
     6001 ccacctaaga ccttccagac tgccatcatt gagattgtaa tgggagacct ccacttcagt
     6061 ctccattcca atcaacgaat ccttaactac gccaaatccg tgattttgac ttgtgatctc
     6121 acccagaccg gtagatgtat tcaaaacggg atgattggta ccccggtgtc cgtatttcag
     6181 tttttcggtc ttcatacctt gagcaagtgc aagcaattga tgccccaggc aaataccgaa
     6241 tacgggtttg ttggaatcaa gaattttcct ggcagacttg atggcatagt ccatggctgc
     6301 cgggtcaccc ggcccattgg aaaggaaata accatcaggc tcccatgcat cgatcgtact
     6361 taattctgtc ctggcaggaa acaccttcag at
//
LOCUS       DAOQMC010000133         6382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5148_length_6382_cov_4.627312, whole genome shotgun sequence.
ACCESSION   DAOQMC010000133 DAOQMC010000000
VERSION     DAOQMC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5148_length_6382_cov_4.627312"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKV31_06400"
     CDS             <1..225
                     /locus_tag="PKV31_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HNB92521.1"
                     /translation="IHDRMPLILNKEEWVEWMDASFTDKMKAIFSDCERMITLVHYKV
                     SSRVNSIKNNDSSLIEPDDSPIYIQKSLFD"
     gene            245..1042
                     /locus_tag="PKV31_06405"
     CDS             245..1042
                     /locus_tag="PKV31_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB92522.1"
                     /translation="MKSIRTTLCMLLVTLFTFQSDAQGGLYLGLNGSGNATIVANQMN
                     YGQKEMDYDAVTFRPAFGLELGYDLNGGSMLSIGANLQQAGQKYKDGYGNGDKLNKEI
                     KMSYFAIPVTYRLVFGGKKDPNHGTKFFVAVGPQISLLQSAEVTHQVNEKEVTLETFA
                     NHIAGGGAPYNFNSAEIGKLTTNGEPANDKDLFASMDIEGFVGLGIQSFLSESFFMTL
                     ELRGGGSLTDINAKDWRLDGYKDGQRQKYEASRNVFAGLRLGLNYKF"
     gene            1116..1760
                     /locus_tag="PKV31_06410"
     CDS             1116..1760
                     /locus_tag="PKV31_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92523.1"
                     /translation="MAKVMARILSFFTHPLWMITFGLFIYLKLDPYAFGVYDWTLRIP
                     FVITVFMMTFFFPALVIFLLLKLDFIKSIELHTARERIIPYIATSIFYLWLYITTRSN
                     PEIAALFKIFVLGTVITLFLIFFINNFSKASAHMAGLAGLLILTIALAYRLGPNDGLG
                     WLTSRPSNLPSMVVLIAGLTGSARLKLEAHTWQELLGGFFIGLLGQLIALKIYF"
     gene            1831..2292
                     /gene="smpB"
                     /locus_tag="PKV31_06415"
     CDS             1831..2292
                     /gene="smpB"
                     /locus_tag="PKV31_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNB92524.1"
                     /translation="MAEKFKNIEIKNKRASFEYFLLQKFEAGLMLKGTEVKAIRLGSA
                     NLTDAYCYIRKGEVFVKNLFISEYSQGSYYNHDARRERKLLLNKTEIRKIERRLSEKG
                     FTLVPTRLYMNERGFAKLEVALAQGKKMYDKRASIKEKDNRRDMDRFKVSR"
     gene            complement(2703..3224)
                     /locus_tag="PKV31_06420"
     CDS             complement(2703..3224)
                     /locus_tag="PKV31_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92525.1"
                     /translation="MKYIFVFFTFFTTLSGGYSQVITDIKPDTTTLKEFEQRIKLTRI
                     DGKYIPKDLNDAMMELNKIMEDGAKKKFAEMSENDARQKTHFSFGKYINARWYIQEGS
                     RLTIWFQKNKIYDFDDMIDCIIVSYHRTLNNKPIEFEILADYYYKKQMEKVKKLVEDK
                     KKREVRVKLNEKE"
     gene            3372..5825
                     /locus_tag="PKV31_06425"
     CDS             3372..5825
                     /locus_tag="PKV31_06425"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNB92526.1"
                     /translation="MKHLGTILSGISLLILIILFNSKWGSIPPLGKLLSPTHGYLIDP
                     ELQGIQSGFSMVELGGLKDSVQVYFDDRMVPHIFASNDEDAYFVQGYLHARDRLWQME
                     FQTHFAAGRLSEIVGEKALVVDRTNRRLGMVYAAEKSLEEMEKDTLTFLAMNAYTAGV
                     NSYIGTLEEKDLPLEYKILDYQPEPWSNLKSALLSKYMAKDLAGYDDDFERTAVIATM
                     GKEIFDLMYPNREDSLDPIIPKAFIPPFGKLTIQAVHDSSYFHPPMPDLIEREKPDKA
                     NGSNNWALAGSKTMTGRPILCNDPHLKLRLPSIWYEIQITTPEYSCYGVSIPGAPGIL
                     IGFNEYVAFGSTNAMRDVMDYYEITFRDKSKSQYLYNGRWESTSSRIETYVIRDAKTQ
                     ADTVFYTHFGPVVYDENFRGLKNDHLRSNAGKFYAVRWKAHDPSNDIVSFLLLDRAKN
                     YDDYRKAIARFSCPGQNLIFASASGDIAITQQGVFPAKWRYQGEFIMEGKDSLYEWQG
                     MIPQDQVLTMKNPERGFVSSANQLPADPKEYPYFLGGDYVYERGYRINQILRKLQKAT
                     VQDMIDLQNDNFNIRAHQIRGLLIETLKKRNFTNEADSYFHILERWNLYNDSASEGAT
                     VYSLLMDSLEQRIWGDELKKSQIGSMPEHSTLIQNLLRNINFACVDNVLTPQVETFDE
                     QLSAAFLSIVPVLDELKKVGRLTWGKYKQTCVPHLMDIKQNMSSLSRLDLNVGGGDGI
                     VNATKKSHGPSWKMIVSLTSPVEAWGVYPGGQSGHPGNPYYDTGVGPWSEGKYFKLWF
                     MKPFEKGSDKIIQTLVFKP"
     gene            5875..6240
                     /locus_tag="PKV31_06430"
     CDS             5875..6240
                     /locus_tag="PKV31_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92527.1"
                     /translation="MNIIKYLIFIIGTLVVSALLTYIGPWWLIFVGPLLFAFFFKISY
                     GKSFLVGAFSIMLFWAIMLLIRSDVFVGSITEKIALLLPLRGSRVALSLVTLVVGGVI
                     GGLAGLNGRSWRRSFGLTQ"
BASE COUNT         1863 a         1128 c         1396 g         1995 t
ORIGIN      
        1 attcatgatc ggatgccctt aatcctgaat aaagaggaat gggtggaatg gatggatgct
       61 agttttaccg ataaaatgaa ggcaattttt tctgattgcg aaagaatgat taccttggtt
      121 cattataagg tgtcatccag ggtcaattct atcaaaaata acgattcatc attgatagaa
      181 ccggatgatt cgccgatata tattcagaaa agcttatttg attaacttac aaaaaataca
      241 aacaatgaaa tcaatcagaa caacactttg catgttactt gttacgttat ttacctttca
      301 atctgatgct cagggaggtt tatatcttgg gctaaatgga agtggaaatg ccaccatcgt
      361 tgccaatcag atgaattatg ggcaaaagga aatggattat gatgcagtca ccttccgccc
      421 tgcatttggc ctggagctgg gatatgacct gaatggaggc agtatgttgt ctattggcgc
      481 caatctgcaa caggccggcc agaaatacaa ggacggttat ggaaatggcg acaagctaaa
      541 taaagaaatc aagatgagtt attttgctat tcccgttaca tatcgcctgg tctttggggg
      601 gaaaaaggat cctaatcatg gaacgaaatt ttttgttgcc gttggtccac agatttctct
      661 tttacaaagt gctgaggtta ctcaccaagt aaatgagaag gaggttacac ttgagacatt
      721 tgcaaatcat atagccggtg gaggtgcccc ctataatttt aattcagccg aaatcggcaa
      781 attgacaacc aatggtgaac ccgccaatga caaggacctg tttgcttcta tggatattga
      841 aggttttgta ggcttgggaa tacagagttt tctatcagag tcatttttta tgacacttga
      901 attgcgcggc ggaggttcat tgaccgatat caatgccaag gattggaggt tggatgggta
      961 taaggatggt caaagacaga aatatgaagc gagtcgtaat gtttttgctg gactgagatt
     1021 aggacttaac tataaattct aggtataggt ttcaaggaag atcccgaatc gatcggaata
     1081 tttcaagaat ttataaacac caaattatca aggaattggc aaaggtgatg gcccggattt
     1141 tgtccttttt tactcatcct ctttggatga tcactttcgg attgtttatt tatcttaaac
     1201 tggatccata tgccttcggt gtctatgact ggacacttcg tattcccttt gttatcacag
     1261 tgtttatgat gacatttttc tttccggcgc tggtcatttt tttgttgcta aagctggatt
     1321 ttatcaaaag catagaatta catacagcca gggaacgtat cattccgtat attgccactt
     1381 caatttttta tttgtggcta tacatcacca cacggagtaa tcctgagata gcggctcttt
     1441 tcaagatctt tgtgcttggc acagtcatta cattattcct tatatttttc attaataatt
     1501 tttcaaaggc aagtgcacat atggcaggtc tggcgggttt gctcatattg accattgcat
     1561 tggcatacag gcttgggcct aatgatggtt tggggtggtt gacatcaagg ccgtccaatc
     1621 tgccatccat ggttgttttg atagccgggt tgacaggaag tgcgaggttg aagctggagg
     1681 ctcacacctg gcaagaattg ttgggtggtt tttttatagg cctgttaggt caattgatcg
     1741 ccttaaaaat ttatttctga ccattcgaac aataatattc attacgggtt acactctaca
     1801 atatagcatt tcgtgtaact ttgcattcct atggctgaaa aattcaagaa tattgaaata
     1861 aagaacaaaa gggcttcctt tgaatatttt ttgcttcaga agtttgaagc cggccttatg
     1921 ctgaaaggta cagaagtaaa ggccatacgc ttaggttctg ctaatcttac tgatgcatat
     1981 tgctatatcc gtaagggcga agtatttgta aagaatttgt tcatttcaga gtatagtcag
     2041 ggctcatact acaaccatga tgctcggcgt gaaagaaaac tattactcaa taagactgaa
     2101 atcagaaaga ttgagcgtcg cctttcggaa aaaggattta ctttggtccc gaccaggctt
     2161 tacatgaatg agcgaggatt tgccaaattg gaggtagcat tggcccaagg taaaaaaatg
     2221 tatgacaaac gggcaagtat caaggaaaag gacaatcgac gggatatgga taggtttaag
     2281 gtaagtcgtt gatggatctc ggcttctgca ttctgccgta aatgcctgat tagtagttgc
     2341 atgtaatttg ctcgtaatat acttctgact attaatatca aaaccaaata cttacattcc
     2401 aaatagtaat tccattatta gcttacaatt aatttaaaga ctcctgcttc ctttgattta
     2461 taggttgacc tattcctcct gcctgaatat agtctcaaca aattctaccg gatcaatatg
     2521 ttggagtcgt ttgatacaaa atttaaatta ttgaagtcaa attgagattg agactcaaca
     2581 gctcttattt ctgctcaaac ttgtaaaact aaacgggcga aaacccttca atcaagcatt
     2641 aaacgttgtt acataaaact ttttgaccaa agtttcaaga taatcaaatc acaggacttt
     2701 agttattcct tttcattgag ttttacccta acttcccttt tctttttatc ttcaaccagt
     2761 tttttgactt tttccatttg ctttttatag taataatcgg caagtatttc gaattcaatg
     2821 ggtttattat taagggtacg atggtaggag acgataatac aatcaatcat gtcgtcgaag
     2881 tcataaattt tgttcttttg aaaccaaatg gtcaacctgc ttccttcctg gatgtaccag
     2941 cgtgcgttga tatatttacc gaatgagaaa tgcgtctttt gtctggcatc attttctgac
     3001 atttcggcaa attttttctt agctccgtct tccatgattt tatttaattc catcatggca
     3061 tcattcaaat cttttggaat gtacttgcca tcgattcggg taagttttat tctttgctca
     3121 aattccttca aagtggttgt atcaggtttt atatctgtaa tgacctgaga atagcctcca
     3181 ctaagagtag tgaaaaatgt gaaaaaaaca aaaatgtatt tcatggtttt ctcttaacct
     3241 ttcaaacgcc gcaaacataa aataaattac ccttatttgt aacaaattga gttattaaag
     3301 gggaaaattt taaatttgtc aaatcaatcg tccaggttga gtgaagtttt ttaaaatatt
     3361 tatgtaataa aatgaagcat ttgggcacca tcctttccgg aatttcacta ttaattctga
     3421 ttatcctgtt caattcaaaa tggggctcca ttcctcctct gggcaagttg ttaagtccta
     3481 cgcacggata cctgatagat cctgaacttc agggtattca atccggattt tctatggtgg
     3541 agcttggtgg actaaaggat tcagtgcaag tctattttga tgaccgtatg gtacctcata
     3601 ttttcgcttc caatgatgaa gatgcttatt ttgttcaagg atatcttcat gccagagatc
     3661 gtctatggca aatggagttt cagacgcatt ttgctgccgg tcgattgagt gaaatagttg
     3721 gggaaaaggc attggttgta gatcgcacta acaggaggct gggcatggtt tatgcagccg
     3781 agaagagtct ggaagagatg gagaaggata cccttacttt tctggcaatg aatgcttata
     3841 cagccggtgt aaatagttat ataggcacat tggaagaaaa agatttgcct ttggaatata
     3901 aaattctgga ttatcaaccg gaaccatggt ccaatttgaa gtcagcatta ttaagcaaat
     3961 atatggcaaa agaccttgca ggatatgatg atgattttga aaggactgct gtgatagcaa
     4021 ccatgggcaa agaaattttt gaccttatgt atcccaatcg tgaagattct ctggatccta
     4081 tcatacctaa ggctttcatt cctccatttg gcaaattaac tattcaggcg gttcatgatt
     4141 cgtcatactt ccatccgcca atgcctgatt taatagaaag agaaaagccg gataaggcta
     4201 atggaagcaa caattgggca cttgccggaa gcaagactat gaccggcaga ccaattcttt
     4261 gcaatgaccc gcaccttaag cttcgcttgc cttccatttg gtatgaaatt cagataacaa
     4321 cacccgagta ttcatgctat ggggtatcca tacccggtgc gccaggtata ttgatagggt
     4381 tcaatgagta tgtagctttc ggttctacca atgccatgcg agatgtgatg gattattatg
     4441 aaataacatt cagggacaaa tcaaagagtc aatatctcta taatggccgt tgggagtcca
     4501 cttcgagcag gatagaaacc tatgttataa gggatgcaaa gactcaggca gatactgtct
     4561 tttatactca ttttggcccc gttgtttatg atgaaaattt ccgtggattg aagaacgatc
     4621 atttgcgttc caatgctggc aaattttatg ccgtgcgctg gaaagcacat gatccaagca
     4681 atgatatagt atcatttctt ttgttggaca gggcgaagaa ttatgatgat taccggaaag
     4741 ccattgccag attttcttgt cccgggcaaa acctgatttt tgcttcggct tccggcgata
     4801 ttgccattac ccagcaaggt gtttttcctg cgaagtggag gtatcaaggt gaattcatca
     4861 tggaaggaaa ggattcactg tatgaatggc agggaatgat acctcaggat caagtattga
     4921 ccatgaaaaa tccggaacga ggattcgtaa gttctgccaa tcaattacct gcagatccaa
     4981 aggaataccc ctattttctg ggtggtgact atgtatatga gagaggatac cggattaatc
     5041 agatactgcg caaacttcaa aaggcgactg ttcaggatat gattgactta cagaatgata
     5101 acttcaacat cagggcgcac caaatcaggg gtcttttgat tgaaactctg aaaaaaagga
     5161 actttaccaa tgaggccgac agctatttcc acattctgga aagatggaat ctgtacaatg
     5221 atagtgccag tgaaggagcg actgtctatt ctcttctcat ggacagtttg gagcaacgaa
     5281 tatggggaga cgaattgaag aagtctcaga taggaagcat gcctgagcat agtacattga
     5341 ttcaaaatct gttgcgaaac attaatttcg catgtgtaga caatgttttg actcctcaag
     5401 ttgaaacatt tgatgagcaa ctgtccgctg catttttatc catagttcca gttttggacg
     5461 aattgaaaaa agtcggtcgg ctgacttggg gtaaatacaa gcaaacctgt gtacctcacc
     5521 tgatggatat caaacagaat atgtcttcat tgagcagact tgacctaaat gttggaggcg
     5581 gagacggaat tgtcaatgca acaaagaaga gtcatggtcc aagttggaag atgattgtct
     5641 cgcttacctc ccctgttgag gcgtggggcg tgtaccccgg cgggcaatcc ggtcatccgg
     5701 gcaatcctta ttacgatacc ggggtaggac cttggtctga agggaaatac tttaaacttt
     5761 ggttcatgaa accctttgag aagggtagtg ataaaatcat tcagacatta gtattcaaac
     5821 catgagagta ttcaaaccac tatatctatc agtacaaatt acaattagac agaaatgaat
     5881 attattaaat atctgatttt cattattggc acattggttg tttccgcttt gttaacctat
     5941 attggtcctt ggtggttgat ttttgtaggg cctttacttt ttgcattctt ttttaagata
     6001 tcatatggca agtcattttt agtaggagca ttttcaataa tgcttttttg ggctatcatg
     6061 ttgttaatta gatctgatgt ttttgtcgga tcgattactg aaaagattgc cttgttattg
     6121 ccgttgcgtg gttcacgagt agcattaagt ttggttactt tagtggtcgg aggtgtgata
     6181 ggtgggttgg ccgggttaaa tggcagatct tggcgtagaa gtttcggttt gacgcagtaa
     6241 atgataatca tccggtgaag gctaacagga agagaggtaa gggagatggg gtgtaggtgt
     6301 gggttaggaa agatgaagtg agtagaagat taatagccac catgcttgac ggtattgcct
     6361 ttcggggcgg ctttgccaaa at
//
LOCUS       DAOQMC010000134         6315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5246_length_6315_cov_4.669329, whole genome shotgun sequence.
ACCESSION   DAOQMC010000134 DAOQMC010000000
VERSION     DAOQMC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5246_length_6315_cov_4.669329"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKV31_06435"
     CDS             <1..676
                     /locus_tag="PKV31_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB92528.1"
                     /translation="LYKKMKVAEQLEYFAALRNMPVQEARQRIGFWLERLNISDWAYK
                     KIEELSKGMQQKIQFIATVLHNPSFLILDEPFSGLDPVNTSLLKDEILRLNQLGTTIM
                     FSTHRMEQVEEICDRIVLISKGKNILDSTVKETKETWKEGLFHLEFEHTIEFTGDMPE
                     GIEIKENKGNELYVKAPNLRNLNTFITSQINKGNLLHSMKERLPTLNEIFIKTVRREN
                     PVPENS"
     gene            complement(685..1815)
                     /locus_tag="PKV31_06440"
     CDS             complement(685..1815)
                     /locus_tag="PKV31_06440"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92529.1"
                     /translation="MRGGRKYLLLFWLIVLIAPLLQSGFKVFKGGALKGAIIPVPDVE
                     FTLKGWFDGSYQQGKEEYLNEHFGFRNDLVRLHNQINFSLFKIASVRDAVVGKENYLF
                     ERKYIEEYYGRNFIGDSAIDRRIFMIQRIADTLNSLNKKLIVVMSPSKARFFPEYIPD
                     SLRGPIVPGNYEKYAEGFSRTGIVTMDCNKYFANLRDTCSYPLFARYGIHWSLYGASF
                     VRDSLLTLIGRVTNKSLPRWDKQFYVSPTPLDTDNDLGDAMNLIVKLKPGPLAYPTYT
                     LPETVQPVPVRLISVGDSFYWTMTSLKIHEALVDDQFWYYNSEVWPPRFGANNNRLTY
                     ADLNIKDEIYNNEVFLLICSEINISNIGFSFVDDAYKLFYGQ"
     gene            complement(1821..3242)
                     /locus_tag="PKV31_06445"
     CDS             complement(1821..3242)
                     /locus_tag="PKV31_06445"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNB92530.1"
                     /translation="MVFSSIVFVLYFLPVFLVLYFIIDRRYKNVLILVSSILFYAWGA
                     PKFIFVILGTTLLDFILVHYMDRMDTDWKRKVMLSISIVVNLGLLVYFKYSNFFIENI
                     NLFLSGLGFNEVAWTKLVLPIGISFYTFETITYVVDVYRRIHRPLTNFWDYQLYIILF
                     PKLIAGPIVRYHEIADQITDRSANENNENRLTGFYRFVIGLSKKVLIANQMGAQADMI
                     FSQDYHQLSMMHAWVGILAYTFQIYFDFSGYSDMAIGIGRMMGFRLPENFNSPYTSGS
                     ITEFWRRWHITLGKWMRNYLYIPLGGNRISSGRTYMNLWLVFVASGLWHGASWNFVIW
                     GAYHGCFLVLERTFLLRLYNRIGRVVPIVLTFFFVVIGWVFFRVEHFNDALTYISRLF
                     AGGMEQFSLPNGRFIAIFLIAVFFAFFAVAQRTYAFQQKVFEQNYSLRGHLLMTPVIL
                     ILFVLSLASITSFGFNPFIYFRF"
     gene            complement(3256..4269)
                     /gene="pdhA"
                     /locus_tag="PKV31_06450"
     CDS             complement(3256..4269)
                     /gene="pdhA"
                     /locus_tag="PKV31_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNB92531.1"
                     /translation="MQSTADKKKTKYTEDTYALWYETMLRIRRFEEKALLMYSQQKIR
                     GFLHVYIGQEAIAAGLKTAMKPEDSMVTAYRVHGLALMKGMTSRECMAELFGKATGCV
                     KGKGGSMHFFAKEHNFYGGNGIVGAQIPIGAGIALAEKYRKTDNICVTLFGDGAARQG
                     ALHETFNMSMTWKLPALFICENNHYAMGTSVERTSVVTDMVKLGLSYEMPSASVDGMN
                     PAAVHEAVTKAIEHIRAGNGPYYLEIKTYRYRGHSVSDPGLYRTKEELEHYKTLDPIG
                     ILEHAIKEEGLFPETRMLEIQSMIEQEIEDAVQFAEESPWPDPSELYTDNYVESDYPF
                     MRD"
     gene            4602..5708
                     /locus_tag="PKV31_06455"
     CDS             4602..5708
                     /locus_tag="PKV31_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNB92532.1"
                     /translation="MENLQLIQFKNYEKLEVSFSEGINIFTGNNGQGKTNLLDAIYLL
                     CMTKSYFQYSEKNLILFDHDFFRVKGIFVKNGSRITIDCKYRLPNNKSFQKDDKEIRK
                     YADYIGSLPVVMITPADIQLIEEGSEERRRFLDVSLCQVDPFYTESLTKYNKFLKHRN
                     SLLKSMNQQQSSENNLLDIYDQEMARLSVIITEYRQRFVSEITGLYENLYSELSGGVE
                     KVTMMYRPDTSGTQEDFINMFRQNLRKDILLERTTHGVHRDDVEFVLDTMPVRKIGSQ
                     GQIKSTIFAAKLSQYQWLRHATHTKPILLLDDIFDKLDHDRVTNLMKIIDSDIYGQIF
                     ITDTYRERVRSILPALRKPSLSFSIQQGRLNHYE"
     gene            5701..6000
                     /locus_tag="PKV31_06460"
     CDS             5701..6000
                     /locus_tag="PKV31_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNB92533.1"
                     /translation="MSKLFSSNEKTFKELMSELVKAPGIRRQYLKVVMKTTWEEEMGP
                     LVVKYTKRLTINKGTLTVSVSSAPLRQELMFNKVKIIEMMNKAFGEELVKEVVIA"
BASE COUNT         2023 a         1388 c         1233 g         1671 t
ORIGIN      
        1 attgtataaa aaaatgaagg ttgcagagca gctggagtat tttgctgcat tgagaaatat
       61 gcctgttcag gaagcccggc agaggatcgg gttctggctt gaaaggctga atatttctga
      121 ttgggcttat aagaagattg aagaactgtc aaagggaatg cagcaaaaga tacagtttat
      181 cgctaccgtc ctgcacaatc cctcctttct tatactcgac gagccatttt caggccttga
      241 cccggtcaat acgagccttt tgaaagatga aatcctgagg ctgaaccaac taggcacgac
      301 aatcatgttc tcgacccacc gaatggagca ggtcgaggaa atatgtgatc gcatcgtatt
      361 gatcagtaag ggcaaaaaca tattggacag caccgtaaag gaaaccaagg aaacgtggaa
      421 ggagggtctc ttccacctcg aatttgagca cacgatcgaa tttacgggtg atatgcctga
      481 aggaattgaa attaaggaaa acaaaggcaa tgagctctat gtaaaggctc ccaaccttcg
      541 caatctgaat acattcatta caagccagat caataaggga aatctactac acagcatgaa
      601 ggaacgcttg cctacgctca acgagatctt catcaaaacg gtgagaagag aaaaccctgt
      661 tcctgaaaat tcttaatcac tcctttattg gccgtagaat aacttgtaag catcatctac
      721 gaatgagaaa ccaatattgc tgatattgat ttctgaacaa atcaacaaaa agacttcatt
      781 gttgtaaatt tcatctttaa tatttaggtc tgcataagtc agtctgttat tatttgcccc
      841 aaaccgcggt ggccacacct cactattata ataccagaac tgatcatcca ccaatgcttc
      901 atgaatcttc aagctggtca tcgtccaata aaagctatcc ccgacggaaa ttaacctgac
      961 cgggacaggc tgtactgttt ccggcaatgt atatgtggga taagccaacg gtcctggttt
     1021 taatttgaca atcagattca tcgcatcacc caaatcatta tctgtatcaa ggggtgtagg
     1081 gcttacataa aattgcttgt cccaacgggg caaagactta ttagttaccc tgccaatcag
     1141 cgtcaataac gaatctctta caaaactcgc tccatacaat gaccaatgaa tgccatacct
     1201 ggcaaacagc ggataggaac aagtatcccg taaattggca aaatacttgt tgcagtccat
     1261 agtaacgata cctgtccggg aaaaaccttc ggcatatttt tcataattgc ccggaacgat
     1321 gggaccacgt aagctatcgg gtatatactc cggaaagaat ctcgccttgc tgggcgacat
     1381 tacgacaatt aacttcttat tcaacgagtt aagtgtatcc gcaatccgtt gaatcataaa
     1441 aatccgccta tctattgcgg aatcaccaat aaaattgcgg ccatagtatt cttcgatata
     1501 tttccgctca aaaagataat tttccttacc taccactgca tcccggacac ttgcaatttt
     1561 gaaaagcgag aagtttatct gattgtgtaa ccttaccaga tcatttctga acccaaaatg
     1621 ttcattgaga tattcctcct tgccctgttg gtaactgccg tcaaaccaac cttttaaggt
     1681 aaactcgaca tcaggaaccg gtatgatagc acctttcaac gctccacctt tgaatacctt
     1741 aaaaccggac tgtaacaacg gagcaatcag aacgatcaac caaaataata acaaatattt
     1801 tcttcctcct ctcatgccta tcagaatctg aaataaatga atggattaaa tccaaaactc
     1861 gtaattgaag ccagggacaa tacgaataat atcaaaatca caggagtcat caggagatgc
     1921 cccctcagag aatagttctg ctcaaatact ttttgctgaa aagcataggt acgctgtgcg
     1981 acagcaaaga aagcaaaaaa gacagcaatc aggaatatgg cgataaatct accattgggc
     2041 agcgaaaatt gctccatgcc ccctgcaaac aacctgctga tgtaagtcag agcatcattg
     2101 aaatgttcca ccctgaaaaa cacccagccg atcaccacaa aaaagaaggt cagaacaatc
     2161 ggaacaaccc ggccgattcg attataaagg cggaggagaa aggttcgctc aagtaccaga
     2221 aagcagccgt ggtatgcgcc ccatataaca aaattccagg atgccccatg ccagagaccg
     2281 gaagctacaa agacaagcca gagattcata taggtacgac ccgaagaaat gcggtttccg
     2341 cccaagggaa tataaaggta attgcgcatc catttgccga gtgtaatgtg ccaacgtcgc
     2401 cagaattcgg taatgctacc ggaagtataa ggactgttga aattttccgg cagtctgaaa
     2461 cccatcattc gcccaatgcc gattgccata tccgaatatc cgctgaagtc gaaataaatc
     2521 tggaaagtat atgccaggat gccgacccat gcatgcatca tcgacaattg atgataatcc
     2581 tgagaaaaaa tcatatcagc ctgcgctccc atctgattgg ctatcaatac cttttttgaa
     2641 aggccgatta caaaccgata aaagccggtc agtctgtttt cattgttttc atttgccgat
     2701 ctatctgtta tctgatcggc tatctcgtga tagcggacaa taggcccggc aattaatttc
     2761 gggaaaagaa tgatgtacag ctggtagtcc cagaaattgg tcagtggcct gtgtattcta
     2821 cggtacacat ctaccacata ggtaattgtc tcaaaggtgt aaaaagaaat accaatcgga
     2881 agaactagtt tggtccatgc cacttcattg aaacctaatc cgcttaggaa aagattgata
     2941 ttctcaataa aaaaattgga atatttgaag tacacgagca ggccaagatt aacaacaatg
     3001 gatatggaca acataacctt tcgtttccaa tcggtgtcca ttctatccat ataatgtacc
     3061 aggataaaat caagtaaagt cgtaccaaga attacaaaga taaacttggg tgcgccccag
     3121 gcataaaaaa gaatagaaga tacgagaatc aggacattct tgtatcttct atcaatgata
     3181 aagtataata ccagaaatac aggtagaaag tataatacaa agactatact gctaaatacc
     3241 atttacccgt cagaattaat ccctcatgaa tggataatca gactccacgt aattatcggt
     3301 gtagagttct gatggatccg gccatggaga ttcttcggca aactgcacgg catcttctat
     3361 ttcctgctcg atcatacttt gaatttcaag cattctggtc tcaggaaaca acccttcctc
     3421 cttgatggca tgctctaata ttccgattgg gtcaagcgtc ttataatgct ctaactcttc
     3481 ttttgttctg tataaacccg ggtcagatac actatgtccg cgatatctgt acgtcttaat
     3541 ttcaaggtag taaggtccat tgcctgcccg gatatgctcg atcgcctttg tcacagcttc
     3601 gtggacggca gcaggattca ttccgtcaac acttgcactg ggcatctcat aacttaagcc
     3661 tagctttacc atatcagtta ccacagaggt cctttcaacc gatgtcccca tggcataatg
     3721 attgttttca caaataaata atgccggaag cttccatgtc atcgacatat tgaaggtctc
     3781 atgcagagcg ccttgccgtg ctgccccatc gccgaacaaa gtaacacaaa tattatccgt
     3841 tttgcgatac ttctctgcta atgcaatgcc ggctccaatt gggatctgtg cacccacaat
     3901 accatttcca ccatagaaat tatgctcttt ggcaaagaaa tgcatggatc ctccctttcc
     3961 tttcacacaa cctgtggctt taccgaataa ttcggccatg cattctctgg aagtcattcc
     4021 cttcatcagg gcaaggccgt ggacccgata ggctgtcacc atgctgtctt caggcttcat
     4081 agccgtcttc aaccctgcag ctatcgcctc ttgcccaatg tacacatgaa ggaagccacg
     4141 aatcttctgt tgggaataca tcaacagcgc cttttcttca aatcgccgga ttctcagcat
     4201 agtttcatac catagcgcat aggtatcctc ggtatatttt gtcttctttt tatcagcagt
     4261 tgattgcatg attctctttt aattaaaata ttgaggggta aaattatgaa tttatcccca
     4321 aatgagacga taaaaaaacg atcattaaat atttacgatt gttatttgca gtgaggtatc
     4381 ccgggcgagg tatgtaaatg tgttaaccaa caaagaaata aatgagtaat tgataattta
     4441 tttacaaaaa agtcaaggaa atcagatgat tgccataaaa attataaaac aaggaaatcc
     4501 accgacatct tgctcccata caacacaatt cagttttttt tgttatctgg gattatcatc
     4561 ttaacttcaa ccttcaaaca atatacattc tgccggtgtt tttggaaaat ttacaattga
     4621 tccagttcaa gaactatgaa aagcttgaag tcagcttttc ggaaggcatc aatattttca
     4681 ccggaaataa tggtcagggt aaaaccaatc tcctggatgc catttattta ctctgcatga
     4741 ccaaatcata ttttcaatat tcagagaaga acctgattct ttttgatcac gatttttttc
     4801 gcgtcaaagg aatctttgta aaaaatggta gccggataac catcgactgt aaataccgac
     4861 ttcccaacaa caaatcattc caaaaggacg acaaagaaat cagaaaatac gcagactata
     4921 ttggcagctt gcctgtggta atgattactc cggccgacat ccagctcatc gaagaaggca
     4981 gtgaggaaag aagacgtttt ctggatgtct cattatgcca ggtcgatcct ttctatacag
     5041 aatctctaac caaatacaac aaattcctga agcatcggaa cagcctgctc aagtccatga
     5101 atcaacaaca gtcctctgaa aacaaccttc tggacatcta tgatcaggaa atggcaaggc
     5161 tttctgtcat catcacagaa tacaggcaac gattcgtctc tgagataacc gggttgtatg
     5221 aaaatcttta ctccgaatta agtggaggag ttgaaaaggt aaccatgatg tacagaccgg
     5281 acacatccgg tactcaagaa gatttcataa acatgttcag gcaaaacctc cggaaggata
     5341 tcctcctgga acgcaccact catggcgtcc acagagatga tgtcgagttt gtgcttgata
     5401 ccatgcctgt caggaaaata ggttctcaag gccagatcaa atccactatt tttgctgcca
     5461 aattatctca ataccaatgg ctcagacatg ctacacatac caaacccata ttgttgctgg
     5521 acgatatctt cgataagctg gaccatgatc gggtgacaaa tcttatgaaa ataatcgata
     5581 gcgatatcta tgggcaaata tttatcaccg atacctatag agagagagtc cgttcaatac
     5641 ttccggcatt gagaaagcct tccctctcct tcagtataca acagggccga ttaaatcatt
     5701 atgagtaagt tattcagttc gaacgaaaag acattcaagg agctgatgtc tgaattggtc
     5761 aaagctcccg gaattcgcag gcaatatctc aaggtagtga tgaagacaac ctgggaggag
     5821 gagatgggac ctcttgtcgt aaaatacact aaaagactga ccatcaataa aggtacccta
     5881 acggtgagtg tatcttccgc tccattacga caggagttga tgttcaacaa agtgaaaata
     5941 atagagatga tgaataaggc atttggtgag gaactggtca aagaagtagt gattgcttga
     6001 gtgagaaaat ggaattagga tgaggagaat gggcaatgaa atgcaagaaa gcaacagatt
     6061 attatcacgc tttaggaaaa ttgaaagatt tacaaatcag cctaatcatg agtcaacacg
     6121 aaacaaggtc ggtcccacac tgaattggtt ccgaaagaat gcctgcaatt cttcatttgt
     6181 tcaatttttg agacatagct acctaatttt ttatctgctt cagaaaaaat tattaccttt
     6241 acgcgcttcc aaaaagcaat ggctccgtag ttcaattgga tagaatatca gatttcggct
     6301 ctgagggttg agggt
//
LOCUS       DAOQMC010000135         6278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5302_length_6278_cov_5.124056, whole genome shotgun sequence.
ACCESSION   DAOQMC010000135 DAOQMC010000000
VERSION     DAOQMC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5302_length_6278_cov_5.124056"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..563
                     /locus_tag="PKV31_06465"
     CDS             207..563
                     /locus_tag="PKV31_06465"
                     /inference="COORDINATES: protein motif:HMM:NF039419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HNB92534.1"
                     /translation="MRLVVTFLAFFIFSAVGYGQIATTVNAKPEPGKKLMTVEASCGE
                     CNFGMDGNNCDLAVRIDGKPYFVDGVAITEFGHPHDKNGFCVAIRKAEVQGEVVNGRF
                     KATYFKLLGIDKKDSK"
     gene            730..1239
                     /locus_tag="PKV31_06470"
     CDS             730..1239
                     /locus_tag="PKV31_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB92535.1"
                     /translation="MNKPLYEFFTSDHRRIEDMLDKATENPDDIRMDYYHEFRTGLLK
                     HIKMEEKILFPAAQKANNGKPIPLAARLRLDHGALTSLMVVPPNAGVIRVIRHILERH
                     DVLEEEPGGMYDICEGLTEKETEYILIQLEAVTEVPVHPHNLAGYALEATKRAVERAG
                     YDYDTIAND"
     gene            1578..2102
                     /locus_tag="PKV31_06475"
     CDS             1578..2102
                     /locus_tag="PKV31_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92536.1"
                     /translation="MDKIRPFTFTSLNDWPYLELIIKIYKEREGVTNRIGKLHIGEEL
                     LLHDVFGSIEYKGPGIFIAGGTGITPFIAIFRALYYSQNLRSIGLIYSNRTIDDIILG
                     QELFKMLGPAYVNVFTRQGVIGFKERKIDRNLLIELIVNFETRFYVCGPKNFTEEISS
                     ILIDLGVNPQALII"
     gene            2138..2953
                     /locus_tag="PKV31_06480"
     CDS             2138..2953
                     /locus_tag="PKV31_06480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB92537.1"
                     /translation="MSIGSRINYFLKRLRKEYKIIVLDEASMVQKKAILTTGMKTLGV
                     VVLLLIIFGVLTTLLTTQTGLLRYLGPRPRAILGNDYLSLKSQIDSLQKLTEIRETKV
                     RTIEKFLTERISSDKGDDMDEDVPSLKSKAKDKDGKASYSTPECLLPLMQFEKPVDGE
                     VTGDFDAGKFHYGIDLAAPSDAEILAVEDGLVVINGYSREDGHVLMISHANGYISWYK
                     HNSRNLVMPGREVIKGQPIAIIGNTGENSSGPHLHFELWQNDVPLNPRSFLKY"
     gene            3153..4415
                     /locus_tag="PKV31_06485"
     CDS             3153..4415
                     /locus_tag="PKV31_06485"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB92538.1"
                     /translation="MIQFTRYVMDNGLTVLLHRDESTPLVVVNMLYKVGSKHESIRKT
                     GLAHLFEHLMFTGTEAVPDFDLPIQRAGGENNAFTSNDITNYYSWAPSDNLEALLFME
                     ADRMARLSLDNEEFEIQKNVVVEEFYETTIDEPYGDMWHLISGMAYHRHPYRWPVIGK
                     RPEDIRKVSHLDAAKFYRHHYYPQNAILVVSGNFKEERARKLIKKHFGTLINPDPHSD
                     SIPKEGVQRKQRRMTVHRKVLVQALYMAFHIPARYDKDFYACDILSDLLASGRSARLY
                     EILVRQKKLFTAIDAYTNGTSDEGLLIIDGRLSEGVTPEEGEAAVWEILEELVIKPVE
                     ERELQKVINKCITTLVFSEYNATNIAMNLAYFESIGNADLINSEFEIYQQLTVRDIRR
                     AAEKVFRKSNSSILYYLSANGHGANLVH"
     gene            4393..4803
                     /gene="ruvX"
                     /locus_tag="PKV31_06490"
     CDS             4393..4803
                     /gene="ruvX"
                     /locus_tag="PKV31_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNB92539.1"
                     /translation="MGRILCIDYGTKRCGIAATDVLQIAVHAVGTVPSGELWLFLEEY
                     LSKEEVEKIVIGYPTHSDGSEFSFMHQIVGLERKIKKIFPQIELDRHDEAFSSARAKE
                     VIIHSGIRKEKRKDKGLVDKISAVLILQDYLKHI"
     gene            4828..5385
                     /gene="def"
                     /locus_tag="PKV31_06495"
     CDS             4828..5385
                     /gene="def"
                     /locus_tag="PKV31_06495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNB92540.1"
                     /translation="MILPIYAYGQPVLKREGDEVSREYPELDKLIDDMFETMYNASGV
                     GLAAPQVGLSLKLFVVDTIQVEKEETRGKGIKKVFLNAELLEETGDEWAYEEGCLSIP
                     DVRGEVDRLPVIRLRYQDEKFEVHEETFEGINARVIQHEYDHTIGKLFIEKLGPVKKQ
                     LIKRKLENIKRGKVSADYRLKFAKF"
     gene            complement(5461..6096)
                     /locus_tag="PKV31_06500"
     CDS             complement(5461..6096)
                     /locus_tag="PKV31_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNB92541.1"
                     /translation="MAKKKPKFYVVWKGRNVGIFDTWDDCKAQVFGFEGAQYKSFDSR
                     EEALTAFQSGYHASIKSPKSIKTSTAKISRTAIVPSSISVDAACSGNPGMMEYRGVMT
                     DTGKEIFHLGPLRDGTNNIGEFLALVHVLALLKQKGREDIPIYSDSKIAIGWIKKGKA
                     NTKLEHTGHNEPIFQLIQRAERWLANNSFANPILKWETDSWGEIPADFGRK"
BASE COUNT         1890 a         1143 c         1438 g         1807 t
ORIGIN      
        1 tatgcaagat ttaaacgaga ctggtacaaa atcccgtata aatatcaaaa tattgtatta
       61 tttattacga agattatcaa aatgttatgg aatatttgtc accaattagt tactgctcaa
      121 ttaatatagg attacattat cttttttttt gaaaatcata gcgacaagcg ggattcattg
      181 attatctttg ccaaaaaaga ttagagatga gattagtagt tacttttttg gcatttttta
      241 tattttctgc agttggatat ggccagatag ctacaactgt aaatgctaag ccggaacccg
      301 gtaagaaatt gatgaccgtt gaagcttctt gtggtgaatg taattttgga atggatggca
      361 ataactgtga tctggctgta cgtattgatg gtaagcctta cttcgttgat ggcgttgcta
      421 tcacagagtt tggtcatcct catgataaga acgggttttg tgtcgctatc agaaaagctg
      481 aagttcaggg tgaagtggtc aacggacgct tcaaagcaac ctatttcaag ctgttgggca
      541 tagacaaaaa ggattctaaa taaatttttt ttatacatta aagcagcatt cagcttcatt
      601 tgaagtgaaa tacgcagatc ggtcaatgtt acaagttgaa cctaaagtgc ctggtaggag
      661 tggtgttcca ttcgggaagg gggaagtgtt ttttatttga aacatagtat tcagtaaatt
      721 attgggagta tgaacaaacc gctatatgaa ttttttacat ccgatcatcg gaggatagaa
      781 gatatgcttg acaaagcaac tgaaaatccg gatgatatca gaatggatta ctatcatgag
      841 ttcagaacgg gtttgttgaa gcatatcaag atggaggaaa agatactttt tcctgctgct
      901 cagaaagcca acaatggtaa acctattcct ttggcagcga ggctccgtct tgatcatggg
      961 gcgctaacct cccttatggt tgtgccgcct aatgccggcg tcatcagagt tattcgtcat
     1021 atattggaaa ggcatgacgt acttgaggaa gagcccggag gtatgtatga tatttgcgaa
     1081 ggacttacag aaaaagaaac agagtacatt ttgattcaat tggaggccgt caccgaagtg
     1141 cctgtacatc ctcacaattt ggcgggatat gccttagaag ccacgaaacg tgcagtagag
     1201 agggctggtt acgattacga taccattgca aatgattgaa aaaatccttt ccaaaccgga
     1261 agtggcatgg tgcaattgta tagaattgaa ggctgatttc cggatgtttt tatatctttg
     1321 aattaggact tcaaagcggg aaatttcaga ttcaatatta gctccggata aaaattgact
     1381 attgggagta ttagtaataa tcttccattt gccggttgaa tccttattta aacaagttag
     1441 gtgctatgct gccattaaaa ataatcggta cacaattcat aacacataat gtaaagcaaa
     1501 taactctgga aaagccgccg gggttcattt atcgtcccgg tcaatcaggt catttttctg
     1561 taaatgaaga gggttgggtg gataagatac gcccttttac ctttacatca ctgaatgatt
     1621 ggccctatct tgaactgatt attaaaatat acaaagaaag agaaggcgta accaatcgca
     1681 tcggaaaact acatatagga gaagaactat tattgcatga cgtatttggc tccatcgaat
     1741 ataagggtcc tggcattttt attgccgggg gcaccggcat aacgcccttc attgctattt
     1801 ttcgagccct gtattacagt cagaatctta gaagtatagg tttgatttat tccaatcgaa
     1861 ctattgatga tatcattctg gggcaggaac tgttcaaaat gcttggaccg gcctatgtca
     1921 atgtatttac cagacagggc gtaattggtt tcaaagaacg taagatagac agaaatttac
     1981 tgattgagtt aattgttaac tttgaaacca gattttacgt ttgtggccct aaaaatttca
     2041 ctgaagagat tagcagtatc ttgatcgacc tgggggttaa tcctcaggca ttgattatct
     2101 gattggctgt tgagtgttca ggccttaata aattataatg tcaataggat ctagaataaa
     2161 ttacttttta aagcgacttc gcaaggaata taaaataata gttcttgatg aagcttccat
     2221 ggtacaaaag aaagcgatac tcacgaccgg catgaagaca ctcggagtgg ttgttctttt
     2281 attaataata tttggtgttc tgacgaccct ccttacaact caaaccggtc tgcttaggta
     2341 ccttggacca aggccccggg ctattttggg caatgactac ctttcactaa agagccaaat
     2401 cgatagcctg cagaagctta cggaaatccg ggaaacaaag gttaggacga ttgagaagtt
     2461 tctcacggag cgtatctcat cggataaagg agacgacatg gatgaagatg taccttcttt
     2521 gaagagtaaa gcaaaagata aagatggaaa agcgagctat tcgactccgg agtgcctctt
     2581 gccattgatg cagtttgaaa agcctgtaga cggagaagtg acgggtgact ttgatgcagg
     2641 taagtttcac tatggtatag accttgctgc accctctgat gctgagatat tggctgttga
     2701 agatgggctg gtggtcatca atggatattc cagggaggat ggccatgttt tgatgataag
     2761 tcatgccaat ggttatatca gctggtataa gcataattcc cggaatcttg tcatgcccgg
     2821 aagagaagtg atcaaagggc agcccatagc cattataggc aatacaggag aaaattcttc
     2881 agggccgcat ctccattttg aattatggca gaatgatgtc ccattgaacc ccagatcatt
     2941 tttgaagtat tgattgaaac cagcatgaaa tgagcccttc tctttttata gagctgtaaa
     3001 tgcttcattg catcgggtct ccaatttgcc agtaacgatt tcctttcaaa aaagcatcga
     3061 ataccataaa ttatttgaag caaatccttt gtatgctaat atgagggaat tgaaattctc
     3121 cattttagtc ccacatcctt atctttgccc gcatgattca atttacgcgc tatgtaatgg
     3181 acaatggact gacagtattg ctccatcgtg atgagtccac tccgctcgtc gtggtcaata
     3241 tgctttataa ggtcggttcc aagcatgaat caatacgaaa aaccggactg gctcaccttt
     3301 ttgaacacct gatgtttacc ggcactgagg ctgtaccgga cttcgacctg cctatccagc
     3361 gtgcaggtgg ggaaaataac gcctttacct ccaatgacat cactaattat tatagctggg
     3421 caccgtcaga taatctggag gcattacttt tcatggaagc ggacaggatg gcacgactta
     3481 gtctggacaa tgaggagttt gaaatccaaa agaatgttgt tgtcgaggaa ttctatgaga
     3541 caacgattga tgaaccgtat ggcgatatgt ggcatctcat atcaggcatg gcttatcaca
     3601 ggcatcctta tcgctggcct gtcataggga agcggcccga ggatatacgt aaggtaagcc
     3661 acttggatgc agccaaattt tataggcatc attattatcc acaaaacgcc attttggtgg
     3721 tgtctggaaa tttcaaagag gaaagggcca ggaagctgat taagaaacac ttcggaacac
     3781 tgatcaaccc agatccccat tcagattcaa ttccgaaaga aggagtgcaa cggaaacaac
     3841 gccgaatgac ggtgcataga aaagtgctcg tacaggcatt gtatatggct tttcatattc
     3901 ctgcgagata tgacaaggat ttttatgcct gcgacatcct ttctgacctg ctagctagtg
     3961 gtcgttctgc gagactgtat gagattttgg tacggcaaaa aaaactgttt actgccatcg
     4021 acgcttatac caatggcacc agtgacgaag gcctgctcat cattgacggt agattgtctg
     4081 aaggtgtgac tcctgaagag ggcgaggctg ctgtatggga aatattggag gaactagtga
     4141 taaaacctgt tgaggaaagg gaattgcaaa aggtaattaa caaatgcatt acgacgcttg
     4201 ttttttcgga atacaatgct accaacattg caatgaacct tgcttatttt gagagtatag
     4261 gtaatgcaga cctgataaac agtgaatttg aaatctacca gcagctaacg gtccgcgata
     4321 tacgcagggc tgcagaaaaa gtattcagaa aatcgaacag cagtatttta tattacttat
     4381 cagcaaatgg acatggggcg aatcttgtgc attgattacg gaaccaagcg ttgtggtata
     4441 gctgctaccg atgttctgca gattgctgta catgccgttg gcacggttcc gagtggagaa
     4501 ttatggctat ttctagaaga atatctttct aaggaggaag tcgaaaaaat tgtcatcggc
     4561 tatccaacac atagtgatgg ctcagaattt tcgtttatgc atcagattgt cggcttggaa
     4621 cgtaaaataa aaaaaatatt tcctcagatt gaattggatc gacacgatga ggcgttttca
     4681 tcggccagag ccaaagaggt catcattcat tcaggtatcc gaaaggagaa aagaaaggat
     4741 aaaggtttgg tggataaaat ttctgctgtg ttaattttgc aggattattt aaaacatatt
     4801 tgacaggata ataattatat atagactatg attttaccaa tatatgcata cggtcaaccg
     4861 gtattgaaga gggaaggtga tgaagtatca agggaatacc cggagctgga caaactgatc
     4921 gacgatatgt ttgagacaat gtacaatgca agtggtgtcg gtttggctgc gcctcaggta
     4981 ggcctgagcc ttaagctttt cgtggtggac acaattcagg tggaaaagga agaaaccagg
     5041 ggcaagggca tcaaaaaagt atttctcaat gctgaattgc tggaggaaac gggtgacgaa
     5101 tgggcttacg aagaaggttg tctttcaatc cctgatgtac gtggagaagt ggataggctt
     5161 cctgttatca gattaagata tcaagacgag aagtttgagg tgcacgaaga gacttttgaa
     5221 ggcatcaatg cccgtgtgat tcagcacgaa tatgatcaca ctatcggaaa attatttatc
     5281 gaaaagttag gtcctgtcaa aaagcaactg atcaagcgaa agctggaaaa tatcaaacgg
     5341 ggtaaggtaa gtgccgatta cagattgaaa tttgccaagt tctgaggttg gcgggaacaa
     5401 tgtcgatgtg agattgaatt gcaaacactg agtaaccatt gaattgctta ctcactgcac
     5461 tcattttctg ccaaaatcag ccggtatttc tccccaacta tcagtctccc atttcaggat
     5521 cggattagca aaactgttgt tggcgagcca tctttctgcc cgctgtatga gttgaaagat
     5581 gggttcattg tgcccggtgt gttccagttt cgtattggct ttaccttttt taatccaacc
     5641 gatagcgatc ttactgtcgg aataaatagg gatatcttcc ctccctttct gtttcaatag
     5701 tgccagaaca tgcaccagcg caagaaattc accaatattg ttggtcccat cacgcaatgg
     5761 accgaggtgg aatatttctt tgccggtatc tgtcataaca ccacgatatt ccatcattcc
     5821 gggattacca ctacatgcgg catcgacact aatggaggaa ggaacgatag ctgttctgga
     5881 aattttcgcg gtagaggtct taatactctt aggactctta atgcttgcat gataaccact
     5941 ctgaaaggct gttaatgcct cttcccttga gtcaaaggat ttatattgag caccttcgaa
     6001 gccgaacacc tgagccttac agtcgtccca ggtatcgaaa atgcctacat tgcgaccttt
     6061 ccagacaaca tagaattttg gttttttctt ggccaaattt ttgtgaattt tacgactaaa
     6121 gataaggaga aacaagtttt ttgaatggga attgtttggc tgtgggtgta aaatgttgta
     6181 ctctgcgtcc atgcagatgt aaagctggaa atatatgcat attataaacc cagattacgc
     6241 aataggattt ttttgattaa aaaaaaaggt gtaatttt
//
LOCUS       DAOQMC010000136         6277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5306_length_6277_cov_5.701864, whole genome shotgun sequence.
ACCESSION   DAOQMC010000136 DAOQMC010000000
VERSION     DAOQMC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5306_length_6277_cov_5.701864"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..1577
                     /locus_tag="PKV31_06505"
     CDS             378..1577
                     /locus_tag="PKV31_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB92542.1"
                     /translation="MVQRPGFLRQFAREWPRTIALVFILVCTLMAFFAYLITSDKTPD
                     VNRQISGIGLKKPGFTVTLLAFPKPGISDNPSFLHFLLNGKEDDHRYLAVSNYKIDGD
                     SLKYSLYEEDAHSGDLPVTTIHKVKWLLGDEVIKIDKSNQLYSITLEDGRHIELSENE
                     VDSRLQQSGIIKRTYLLGTDLFGRSLLDRIILGIRYSLFIGFLAVLLSITIGTVIGML
                     SGYFGGKTDDVLSFLMNVAWSIPTLILVFAIVLVTGRGIQNIFIAVGLTMWVDAARLV
                     RGQVLSLKKEKYVEVARTMGMSTPRILFRHILPNMLGPVIVIAVSNFASAIIVESGLS
                     YLGFGIQPPTPSLGSILSENYGFILTGKPMLAIIPVVILLLLVLSFNLVGNGVRDIFD
                     VKSNLQP"
     gene            1581..2819
                     /locus_tag="PKV31_06510"
     CDS             1581..2819
                     /locus_tag="PKV31_06510"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNB92543.1"
                     /translation="MVDDTLIWQSKVRNLEKNLYYKDIQVSYLSQITREINENASSER
                     LFHRFAGFLSTEMAVDRMLLLFRDDDGWKIRSSIGFDELPEQDFEPILSSYNSPGFLL
                     KKDKKMLPGMEYIIPVKHKDTAIAYALIGHFGSELRTHDNYEFVATLANIIAVAIENK
                     RLFNRQLEQERFKNEMKLATEVQQMMLPGKWPECTSFEVSSIYRPLWNIGGDYLDCIK
                     IDKNRTAFCIADVSGKGIAAAMLMANFQAVLHQVIKSSVGLEDIVRELNRAVIRIANT
                     EKIVTFFIGEYNSKTKTLRYINAGHVPPVLYMDSEILFLDQGTTLLGAVEELPDLRPG
                     TLHIHSDAMIVLYTDGITDLKDPGGRSFEDEILFDFVRKNADRKVREFNDGLLQILEQ
                     FNDADKFPDDIAVLTCKICA"
     gene            complement(3049..3960)
                     /gene="menA"
                     /locus_tag="PKV31_06515"
     CDS             complement(3049..3960)
                     /gene="menA"
                     /locus_tag="PKV31_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HNB92544.1"
                     /translation="MANISSWLTAFRLHTLPLALSTIIMGSALAYDRGSYNGMVTLWA
                     CLTTVFLQILSNLANDYGDTASGVDNADRIGPKRGLQSGDITKGQMKKAIYSFIALSL
                     ISGILLIMNGTRELRITYGLILFIIGIGAIAAAMKYTMGKNPYGYSGYGDIFVFLFFG
                     LVGVLGTYFLHTHNLVSIIVLPAIAMGCLSAGVLNLNNLRDRDNDAASGKHTLVVKLG
                     LKNAKRYHAILLITAMLAMIAFSILSGGGLKYWLYLPSFIGIIRSIVVVFRNEDPVAL
                     YPELKNLALNTLSLSLMVGVGALLTGM"
     gene            complement(3972..4814)
                     /locus_tag="PKV31_06520"
     CDS             complement(3972..4814)
                     /locus_tag="PKV31_06520"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNB92545.1"
                     /translation="MKNIPWETVKEYQDITFRYHNGVARLAFNRPEVRNAFRPETVSE
                     LLEALIICHESQEIGVVLISGEGPSPKDGGWAFCSGGDQRVRSKTGYKGGDGINRFNI
                     LDVQRQIRFMNKVVIAVVPGWAVGGGHSLHVVCDLTLASKEHAIFKQTDVDVASIDGG
                     FGSAYLARQIGQKRAREIFFLGKSYSAQEAFEMGMVNAVIAHEELEYTAYEWAQEILA
                     KSPTAIKMAKFALNLIDDGLVGQQVYAGEATRLIYMTEEAEEGRNAFLEKRKPGFDKF
                     PKFP"
     gene            complement(4818..>6277)
                     /gene="menD"
                     /locus_tag="PKV31_06525"
     CDS             complement(4818..>6277)
                     /gene="menD"
                     /locus_tag="PKV31_06525"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNB92546.1"
                     /translation="TLNFAPAISEAFYRRIPLIVLTTDRPAEWTDQGDGQTIRQSHIY
                     RNFIRHSYDLNGDADSYDQLWHIERCLCEGWNIATMADPGPVHFNIPLKEPLYITSEE
                     EDDSNVRTFRELSLDRQLDHGSLKTMAAIFDNIKKVMVLVGQHDVDHELSAGLTELAA
                     HDNVVVLTESTSNVHHPHFVENIDRLITGLNDVDAAELMPDLLITIGDAVVSKRIKGL
                     LRKYRPSHHWNIHRFDALMDTYQSLSLAIPLSGSGFLNQFNQAVSGTSSDYNKRWQTL
                     KERKKQLHDEFTVNSEYSDYSAFNRIVQAIPKDWVVHVANSSPIRYVQLFDNSHIADM
                     QCNRGTSGIEGCTGTAMGAASASPDKKFLLITGDIAFHYDVNALWNEARIENLNIIVI
                     NNAGGGIFRIIEGPDKAAERLAFIETEMHLDAERIAAHHDWNYLKALDNESLDTALKR
                     LFDGNTGRTILEIFTDADKNPLVLGQYWNYLKEKN"
BASE COUNT         1694 a         1394 c         1384 g         1805 t
ORIGIN      
        1 taggtaggaa agtaataatt caaaaaccgc tatacacaag gatttggagg caaatagaaa
       61 atttaattcc cgaataaaat atctgaattt tggcaaagcc gccccaaaag gcaataccat
      121 caagcattat ggctattaat catctaccta cttcagaggc ggcaccaccc ccaaacaata
      181 attagttttc ttatcgatta taccatttct atcttatttt actaattgtt ggtagctgca
      241 catcttttca ccttaatagt tttagaagtg catgttgata tttccttacc ttgatattat
      301 cactattcat tttgttaaaa tattcaataa ttatcgttaa cttatttgtt actttacaga
      361 tttaaatgca ataacatatg gtacaaaggc ctggcttctt aagacaattt gcacgggaat
      421 ggccgcgcac cattgccttg gtattcatct tggtctgtac actaatggcg ttttttgcat
      481 acctcatcac aagtgacaaa acacctgatg tcaacagaca aatttccggt atcggactga
      541 aaaagcccgg ctttacagtg actctactgg ccttcccaaa accaggtatt tctgacaatc
      601 cctcttttct gcatttcctg ctgaatggaa aggaggacga tcaccgctat ctggctgtgt
      661 ccaattataa gattgatggt gatagtctta aatacagcct ttatgaggaa gatgcccact
      721 ctggagactt accagtaact acgatacaca aggtcaagtg gttattgggc gacgaggtaa
      781 ttaaaattga caaatccaat cagctatact ccataacctt ggaggacggt cgacatatcg
      841 agttaagcga aaacgaagtg gatagccggc tacaacagtc cggcatcatt aaaagaacat
      901 atctgctggg cactgatttg tttgggcggt cactgctcga caggattatt ctcgggatcc
      961 ggtatagcct tttcatagga tttcttgctg tcctgctttc cattacaatt ggtacggtca
     1021 ttggtatgtt gagcggatat tttggaggca agacagacga tgtcctcagc tttctgatga
     1081 atgttgcctg gagcataccg accttgattt tggtttttgc catagtgctg gtaacaggac
     1141 gcggtattca gaacatcttc atagcagtag gcttgactat gtgggtggat gcagcaagac
     1201 ttgtccgcgg tcaggtactg tccctgaaaa aggaaaaata tgtcgaagtc gcccgtacta
     1261 tgggtatgag tacaccacgc attctattca ggcatatatt gccaaacatg cttggcccag
     1321 tcattgttat tgcggtttcc aactttgcct ccgctatcat agtggagagt ggcttaagct
     1381 atctgggctt cggtatacaa ccacccactc cttctctcgg ctccatcctg agcgaaaatt
     1441 acggatttat tctgacaggc aagccgatgc tggctatcat cccggtagta attttattgt
     1501 tgctggtttt gtcatttaac ttggttggaa acggagtaag agatatcttt gatgtaaaat
     1561 ccaatttaca accctgatcc atggtggacg atactttaat ctggcaaagt aaggtaagga
     1621 acctcgaaaa gaacctttat tacaaggata tacaggtcag ctatctgtcc cagattactc
     1681 gtgagatcaa tgaaaatgct tcttcagaac gcttgttcca tcgctttgcc ggttttttga
     1741 gcaccgaaat ggccgtggat aggatgttgt tgttgtttcg ggatgatgat ggatggaaaa
     1801 ttcgctcaag catcggtttt gatgaattgc cggaacagga tttcgaacca attctgtcat
     1861 cctacaatag tcccggcttc cttttgaaaa aagacaaaaa aatgctcccc ggtatggaat
     1921 atatcattcc ggtaaagcat aaggatactg caatagcata tgcgttgatc gggcactttg
     1981 gttccgagtt gaggacacat gacaattatg agtttgttgc gacccttgct aatatcattg
     2041 ccgtagcgat tgaaaacaaa agactcttca accgtcagtt ggaacaggaa cgcttcaaga
     2101 atgaaatgaa gctggctaca gaagttcagc aaatgatgtt gccgggcaaa tggcctgaat
     2161 gcactagttt tgaagtttcg tcgatttatc ggccgttgtg gaatataggt ggtgactatc
     2221 ttgattgtat caaaattgat aagaatcgta cggctttctg tattgcagat gtttccggaa
     2281 aaggtatagc agcagctatg ttgatggcca attttcaggc ggtattgcat caggtaatca
     2341 agtcgagtgt cggcctggag gatattgtgc gcgagcttaa cagagcggtt atccggatag
     2401 ctaataccga gaagattgtc acctttttta taggtgaata taacagcaag acaaaaacgc
     2461 tgcgttacat caatgcaggt catgtgccgc cagtcttata catggatagc gaaatactat
     2521 ttctcgatca ggggacaacc ctgcttggag ccgtggaaga actacctgac ctgcgtccgg
     2581 gcacattgca tattcattcg gatgcaatga tcgtccttta taccgacggc ataacagatc
     2641 ttaaggatcc gggaggcaga tcgtttgaag atgagatcct ttttgatttt gtaagaaaga
     2701 atgccgatag aaaggtaagg gaattcaatg atggcctgtt acaaatcctg gaacagttta
     2761 atgatgctga taagttcccc gatgatattg ccgttctgac ttgcaaaatt tgtgcttgag
     2821 acagggttat tgaatcatct tttaatttgt tgaaaaaatc ttgttgtaaa aacattgtac
     2881 actacttcac cagtcggtga tgaggttttc tttgacagga ttttttgcgc cccttattat
     2941 cttttacctg ataccgggat taggagggtt caataatttg attgcctctg ggaatacgct
     3001 gtaaattttt tattttcatt ttttatgaat cttagattca acaaacgatc acattccggt
     3061 taataaagct cccacgccaa ccattaaact aagtgacaag gtattgaggg caagattttt
     3121 aagctcagga tacaaggcta cagggtcttc attgcggaag acaacaacga tacttctaat
     3181 gatgccgata aatgaaggta gatagagcca gtacttcagg ccaccacccg acagaatgct
     3241 gaaagcaatc atggctaaca tggctgtgat aagaagaatg gcgtggtagc gcttggcatt
     3301 tttgagtccc agtttgacca caagcgtatg cttcccggag gcggcatcat tgtcacggtc
     3361 ccggagattg ttcagattaa gcactccggc gctgaggcag cccatcgcta tggcaggaag
     3421 gacgataatc gacaccaaat tgtgggtgtg cagaaagtaa gtgcccagca caccaaccag
     3481 accaaaaaac aagaaaacaa agatatcacc ataacctgaa tagccataag gattcttacc
     3541 catagtgtat ttcatggctg ccgcaatggc accgatacct atgatgaaga gaatcagacc
     3601 ataggttata cgtagttccc tggtcccgtt cataatcaaa agaatgcctg atataaggga
     3661 aagtgcaatg aaagaataaa tagccttctt catctggcct ttggtgatgt ctcccgattg
     3721 cagacctctc tttggcccga tccgatccgc attgtccaca ccacttgctg tatctccata
     3781 gtcgttggca aggttgctca gtatctgcaa aaagacagtc gtcagacacg cccaaagggt
     3841 caccattcca ttataactgc ctcgatcata cgctaaggcg ctgcccataa tgatggtgga
     3901 taaggccaat ggcagggtat gcagccggaa ggcagtaagc caggaagaga tatttgccat
     3961 gacaaccgga atcaggggaa tttagggaac ttatcgaatc caggttttct cttttccaga
     4021 aaagcatttc ggccttcttc tgcctcttcg gtcatgtaga tcaatcgagt ggcctctccg
     4081 gcatatacct gctggcctac cagcccgtca tcgatcagat taagggcaaa tttggccatc
     4141 ttgatagctg tagggctctt ggcaagtatt tcctgtgccc attcataggc tgtatattcg
     4201 agctcttcat gagcgataac ggcattgacc atgcccatct caaaagcctc ttgcgcggaa
     4261 taggatttgc cgaggaagaa tatctcccga gctcgttttt gccctatctg acgggccaga
     4321 taagctgatc cgaacccccc gtcaatactg gctacatcca catccgtctg tttaaatatc
     4381 gcatgttcct tactcgctaa ggtcaggtca cataccacgt gaagactgtg gccaccaccc
     4441 actgcccatc cgggcactac ggcaatcact accttgttca taaacctgat ctgccgctgt
     4501 acgtccagga tattgaaccg attgattcca tcacctccct tatatccagt ctttgaacgt
     4561 acccgttggt caccaccgga acagaaagcc cagcctccat ccttgggcga aggcccttca
     4621 ccacttatca gtacgacgcc aatttcctgt gattcatggc aaatgataag cgcttccaac
     4681 aattcggata cagtctccgg tctaaaagca ttccgcacct ccggccgatt gaatgcgagc
     4741 ctggcgacgc cgttgtgata ccggaatgtg atgtcctgat attctttgac tgtttcccag
     4801 ggtatatttt tcatcgttca attcttttct ttcagataat tccaatattg acccaaaact
     4861 aacggatttt tgtcagcatc agtaaatatt tccaggatag ttctacctgt atttccatca
     4921 aataaccttt ttagtgccgt atcaaggctt tcgttatcga gcgcctttag gtaattccag
     4981 tcatgatgtg ccgcaatacg ttcggcatcc aaatgcattt cagtctcaat gaacgccaac
     5041 ctttcggccg ccttgtccgg gccttcaatg atgcggaaga tgcctccgcc tgcgttgttg
     5101 atgactataa tatttagatt ttctatccta gcttcattcc acagggcatt gacgtcataa
     5161 tgaaatgcaa tgtcaccggt gataagcagg aattttttat cgggtgaggc agaagccgct
     5221 cccattgctg ttcccgtaca tccttcgatg ccgctggtac cgcgattaca ctgcatgtct
     5281 gcgatgtggc tattgtcaaa caattgtaca tatctaatcg gtgaactgtt ggcaacatgc
     5341 actacccaat cttttggaat tgcctgtaca attcggttaa aagccgaata atcactgtat
     5401 tcgctattta ctgtaaattc atcgtgcagt tgcttttttc tttcttttaa tgtctgccat
     5461 cgcttattgt aatccgatga cgtccctgat accgcttgat tgaactgatt caaaaagcca
     5521 gaaccggaaa ggggaatggc gagtgagagc gattggtagg tgtccattag tgcgtcaaag
     5581 cgatgtatat tccagtgatg agagggacga tatttgcgga ggaggccctt tatccgttta
     5641 gatacaacag catctccaat tgtgatcaac aggtccggca ttaattctgc tgcgtctaca
     5701 tcattcaacc ctgtgatgag ccggtcgatg ttctcgacaa aatgaggatg gtgtacattg
     5761 gaagtggatt ctgtcaatac tacaacattg tcatgagcgg caagctctgt taagcccgct
     5821 gataattcgt gatccacatc atgttgacct accaatacca ttaccttctt tatattgtca
     5881 aatattgctg ccatcgtctt caaacttccg tggtccaact gtctgtcgag actcaattct
     5941 cggaatgtac gcacattgga gtcgtcctcc tcctccgaag ttatatatag cggttccttc
     6001 aagggtatat tgaagtgaac ggggccggga tccgccattg tggctatatt ccaaccctca
     6061 cataggcagc gttcaatgtg ccacagttga tcgtatgaat cggcgtcacc atttagatca
     6121 taactatggc gtataaaatt tctgtagatg tggctttggc ggatagtttg cccgtctcct
     6181 tgatcggtcc attctgcagg tctgtcggta gtaagaacga tgagtggaat ccgtcggtaa
     6241 aatgcttcgg atatagccgg tgcaaaattt agggtcg
//
LOCUS       DAOQMC010000137         6255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5349_length_6255_cov_4.599032, whole genome shotgun sequence.
ACCESSION   DAOQMC010000137 DAOQMC010000000
VERSION     DAOQMC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5349_length_6255_cov_4.599032"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PKV31_06530"
     CDS             <1..1131
                     /locus_tag="PKV31_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92547.1"
                     /translation="VTGGTEPYNFKWSNGADVQNLNQVKSGKYTLTITDAAGCQSGGF
                     FSINQPPPFKKTLTVTDATAADQFDGKVKFVFSGGVPPYTLIYDVTDTLTTYTGIVTF
                     ENQLAGLHSIYVEDANGCVQSEFFIINIIGCQLEPSNVVIQDVKCNGGSDGAICVGVS
                     GATGAYDLYWDDETTGPCLLELEAGMYSLYITDEAGCQSLDTFTIRQPSILREINSHV
                     IKPDPGLNNGSIAVFNTGGIPDYTYTWYKDSVRIDGNGSLISGLGTGIYYYELQDANG
                     CGFVSDTFDLFVPTSSKHSITDSSLKLYPNPTNGSIYLDFGLSESSGVRVFNATGMQV
                     AVRVIRNQYRPEIDLDKLAPGVYTIIVDHEKGRSVGRIVLMR"
     gene            2025..3014
                     /locus_tag="PKV31_06535"
     CDS             2025..3014
                     /locus_tag="PKV31_06535"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNB92548.1"
                     /translation="MSTKIKVAILAGGDTEERVVSVKSAGVVHKYLHGEKYDARIIDI
                     NGREWIDLDTGHSIDKNDFSLDLGGSKWKPEVVFCAIHGAPMENGIIQGYFDILGIPY
                     TCCDGFVSGLTMNKSLTKRTLQAYDIPMAREVLVTKAAYREGVDTEDIIAKLGLPLFV
                     KPNAHGSSFGVRKVKNIEELIPAIEYAFQFDQDVMVESMMAGREFGNGVMRINGEIVV
                     LPVTEIIPLKEFFDFEAKYEGASREVTPAEISSELERQVKEYTSKIYTALNCSGFVRV
                     DYILVGDTFHLIEVNTVPGLSEASIIPQQAIAHGWTLNQFFDLAVSEALREKS"
     gene            complement(3079..4176)
                     /gene="hisC"
                     /locus_tag="PKV31_06540"
     CDS             complement(3079..4176)
                     /gene="hisC"
                     /locus_tag="PKV31_06540"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNB92549.1"
                     /translation="MVIIPPHIENLAPYKPGKPGANMFEDAKPEREVVLSSNENNFGP
                     SPKAMEALKRSLNNMNLYPDPTASLLKERLSHLLDIPVSHLLMSNGSDAILYTMFRAF
                     LSGDDSMLTSHAAFVSAKVMARMNNIRCVEVAMTGDHEFDLDGIITNIDDTTKLIYLV
                     NPNNPTGQMMTRTAMDSFLSRVPQHILVVVDEAYCEFARALSGEYPDCTRLGYNNVIV
                     LRTFSKAYGLAGLRLGYAIGPEYLIQALTKVKLTFDPNLAAQAAGLAALDDTTFLDKT
                     IENNTTELQKLNQAYDRLGLKVIPSVANFITIKLKNEAEVENYYHQLLSRGVLTRRLA
                     SFGLPYCLRISIGKPDENDYMLRMLEEVMDQ"
     gene            4464..5036
                     /locus_tag="PKV31_06545"
     CDS             4464..5036
                     /locus_tag="PKV31_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNB92550.1"
                     /translation="MNTIAINGNKRNEVGKKASKAVRSSEGIPCVLYGKNGTTHFTTQ
                     EAEVRHLVFTPEFKVAELNVDGAKSKAILKDIQFHPVKDTITHIDFLELTEGNPVKVE
                     IPLKTKGSSPGVKVGGKLIQSVRKIKVKTVPEKLVGELFVDISGLELGQSLRVKDIIA
                     QDGIEVMQNGSIPVVSIEIPRALRSAAGKE"
     gene            5226..6086
                     /locus_tag="PKV31_06550"
     CDS             5226..6086
                     /locus_tag="PKV31_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNB92551.1"
                     /translation="MKLIRFGQKGKEKPGILLDGKYRDVSSYIHDYDETFFENDGLQR
                     LEQIIHSSTTTMAEIPQDTRLGSPVARPSKIVCIGLNYKNHVMETNANIPTQPILFFK
                     STTALSGPNDDVMIPRDSVKTDWEIELAVIMGRRASYIEPEEAYDYIAGYSLINDYSE
                     RAFQKEMGGQFCKGKSCDTFAPMGPFLATKDEVEDVHNLSLTLSVNEIIRQQGSTADM
                     IFDIPYLVSYISRFMTLLPGDILCTGTPEGVGAGIKPEPVFLRSGDVVRMSIDELGSS
                     TQRVIAYSGG"
BASE COUNT         1898 a         1171 c         1396 g         1790 t
ORIGIN      
        1 gtgacaggtg gtacagagcc ttataatttt aagtggtcca atggagcgga tgttcaaaat
       61 ctgaatcagg tcaaatccgg taaatataca ctcacaataa cagatgcggc gggctgtcag
      121 tccggagggt ttttcagtat caaccaaccc ccgccattta aaaaaacatt gacagtgaca
      181 gatgcaacag cagccgatca gtttgacggg aaggtaaagt ttgtcttttc cggaggagtg
      241 cctccttata cattgatcta tgatgtaacg gatactttga ctacctatac cggtatagta
      301 acatttgaga atcagttggc agggctgcat agtatttatg ttgaagatgc caatggttgt
      361 gttcaaagtg aattttttat catcaatata attggctgcc agctggagcc atccaatgta
      421 gtgatccagg atgtaaaatg taacggagga agcgatggtg caatttgtgt tggtgtcagc
      481 ggtgccacag gtgcttatga cctgtactgg gatgacgaaa ccaccggtcc atgcctgctt
      541 gagctagaag ccggcatgta tagcctgtat atcactgatg aagcaggttg ccaaagtctg
      601 gatacattta caataagaca gccttccatt ttgagggaaa tcaacagcca tgtcatcaaa
      661 ccggatcccg gactgaacaa tggatctatt gctgttttca acaccggtgg gattcctgat
      721 tatacttata cctggtataa agacagtgtc cgtatagacg gtaacggttc attaatatca
      781 gggcttggaa ccggtattta ttattatgaa ttgcaggatg ccaatggatg tggatttgta
      841 tcggatactt tcgatctctt cgtacctacc agttcaaagc attctattac agactcatcg
      901 ttaaagttgt atcctaaccc aacaaacggc tcaatctatc tggatttcgg cttatcggaa
      961 agttccggag ttcgcgtatt caatgcaaca ggaatgcagg tagctgtcag agtaattcgc
     1021 aatcaatacc gcccggagat agatttggac aaattagcac cgggagttta tacaatcatt
     1081 gtagatcatg aaaaaggtcg cagtgttggc agaattgtac ttatgcgtta attgacaatt
     1141 taaaataaat ggggataata tgctaaagat tatatagtcc tatgaattat cccttttttt
     1201 taatgcatgc gttgctgtct gtgttgggta tgataattgg gcaagtgaat tcatatcaca
     1261 ctccgtatag aacacgggga aagatgggcc ccaaataata aatcagaaca ggcagtccat
     1321 ctattcctat aaaattccgg taagccaaaa ggttcaggca aatgcttccg gttcttgata
     1381 ttgcgaagca tttcactgaa cagaattgaa ataaaaatta acaaaccaca atcggtatta
     1441 ccggaatctt attttgaatt ggcgggagga aatatggcat gaatgatatc agggttgagt
     1501 aagtaggaca gaagtcgggc aattcaacct gtattaggta catgtttaat agatgaaaca
     1561 tagaagagtc ggtaactttg ggatagacag gccggaaatg attacttagc gaatcaattt
     1621 atagaaattg actttcgggt ttcccgagta gttcgcgttt tgaggatatt attttcatac
     1681 atacttcgat ttttgaatac gattcagatt ttgccgggct tccactttca atccattcag
     1741 actgcaatca gattctacct gatggatgta tgcaaattag tggatatttc ttcaaagtaa
     1801 taccgaaggt acagtcgtat agatcaatga aattggccta tacgactgta accccggcat
     1861 cccggaaccc cggattgttt cactcccggg acagagcaga gctttagtag catagctatc
     1921 cggacaagta tactaaaaac ttatgggcta ttatataaat catcttagta taatttatta
     1981 attccagcag aattatataa ttttacacat taaataaaat tattatgtca accaagatca
     2041 aagtagcgat tctcgcaggt ggagatactg aagagcgggt agtatccgtc aagagcgccg
     2101 gagtcgtcca taaatatctt catggtgaaa aatatgatgc gcgcatcata gatatcaatg
     2161 ggcgtgaatg gatagatctt gataccgggc attcaattga taagaatgat ttttccttgg
     2221 atctgggcgg gtcgaaatgg aagcctgaag ttgtcttttg tgccatccat ggtgcgccca
     2281 tggagaatgg cattatccag ggttattttg acatccttgg aataccttat acttgttgtg
     2341 atggttttgt ttcgggactt accatgaata aatcccttac taaaagaaca cttcaagcct
     2401 atgatatacc tatggccagg gaagttttgg tgacaaaagc agcatatcgt gagggcgtgg
     2461 atacagaaga catcattgca aagctgggac ttcctctatt tgtaaaaccc aatgcacatg
     2521 gttccagttt tggagttagg aaggtaaaga acattgaaga gttaattccc gcgatagaat
     2581 atgctttcca gttcgatcag gacgtgatgg tagagtctat gatggcggga cgtgaatttg
     2641 ggaatggagt catgaggatc aatggagaga ttgtcgtgct tccggtgact gaaatcatcc
     2701 cactgaagga gttctttgat tttgaagcca aatatgaagg agcatcaaga gaggtcactc
     2761 ctgcagagat ttcatccgaa cttgaacgac aagttaaaga atatactagc aaaatatata
     2821 cagcactaaa ttgttcaggc tttgtaaggg tagattatat tttggtaggg gacacattcc
     2881 atctcataga ggtcaacacc gtcccggggc tatccgaagc aagcattatt ccacagcagg
     2941 caatagccca tggctggacg cttaatcaat tttttgacct tgcggtaagt gaagcactcc
     3001 gggaaaagag ttgaaaccca ggtttagatt ctttttactt ttcttaatca attagagctc
     3061 ctggctgaac aaaaagaatt attggtccat aacctcttcc aacatccgta acatgtagtc
     3121 attctcatca ggcttgccaa tggaaatcct aagacaataa ggtagaccaa atgatgctaa
     3181 gcgtcttgtc aatactccac gagataataa ctgatgataa tagttttcca cttcggcttc
     3241 attctttagt ttgatggtaa tgaaattggc tacggagggt atgactttca atcccaggcg
     3301 gtcataagct tggttaagtt tctgtaattc tgtggtattg ttctcaatgg ttttgtctaa
     3361 gaatgtcgtg tcatctaaag cggctaatcc ggcggcctgt gctgcaaggt tgggatcgaa
     3421 ggtcaatttg actttagtaa gagcctggat caaatattca ggaccaattg cataacccaa
     3481 ccgtaaaccc gctaagccgt aagcttttga aaaagtcctc aacacaatga cattattata
     3541 tcccaatcgg gtacagtccg ggtattctcc ggaaagcgcc cgagcaaact cgcaataggc
     3601 ttcatcgact acgaccaaaa tgtgttgagg aacacggctt aggaatgaat ccatagctgt
     3661 ccgcgtcatc atctgaccag tcggattatt tgggttgacc aaataaatca acttagtagt
     3721 atcatcaatg tttgtgatta taccatccag atcaaactca tgatctccgg tcatagcaac
     3781 ttccacacaa cgtatattgt tcatacgagc catcactttg gcggatacaa aggcggcatg
     3841 agaagtgagc atgctgtcat ctcctgacaa aaaagccctg aacatagtgt aaaggatagc
     3901 gtccgaacca ttgctcatca gtaggtggct cacaggtata tccaacaggt gggacaggcg
     3961 ttccttgagt aaggatgcag tcgggtccgg gtacaaattc atgttattaa gcgacctttt
     4021 caaggcttcc atcgcttttg ggcttggccc gaaattattc tcattgctgc tcagcacaac
     4081 ttcacgctcc ggcttggcat cttcaaacat attagcacca ggctttccgg gtttatatgg
     4141 ggctaaattt tcaatatggg gcgggataat aaccatggat attaaaattt gagtaaataa
     4201 accggacctg gactggtctt taaggtaatc ttatttgagt tgcaaatata ttgaaatatt
     4261 catcttcgat tggtaatggt tcaatcgtta tggcaagaag tcaatgattt tttaatccac
     4321 cattacatta tcctacttcg ttacaactat ccaattctgt catcggacat aatcaacaga
     4381 tttttaaaga attattttgt caatttgtaa aacaatttta cctttgcact ccgatattaa
     4441 aaagggcatt taaatatacg acaatgaata ctatagctat caacggaaat aagagaaatg
     4501 aagtaggaaa aaaggcctca aaggctgttc gatcttcaga aggaattcct tgtgtacttt
     4561 acggaaaaaa tgggacaact cacttcacca ctcaagaggc tgaagttcga catcttgtat
     4621 tcacacctga attcaaagtt gccgagttga atgttgacgg tgcaaaatcc aaggctattt
     4681 taaaggatat ccagtttcat ccggtaaaag ataccattac gcacattgat ttcctggagt
     4741 tgacagaggg caatcccgtt aaagttgaga ttccgcttaa aacaaaagga agcagtcccg
     4801 gagtaaaagt cggtggtaag ttgatccaaa gtgttcgtaa gataaaggta aagacagttc
     4861 cggaaaaatt ggtaggagaa ttatttgtcg atatttccgg attggaactg ggacaatcat
     4921 tgagggtgaa ggatatcatt gctcaagatg gaattgaagt tatgcagaac ggaagtattc
     4981 ctgttgtaag cattgagatt ccacgtgctt tgagaagtgc tgcaggaaaa gaataatttc
     5041 aatagaaata gttattaagc atcaaaaaag aggccggtaa taccggcctc ttttttttgt
     5101 gaatttcaaa tcaggtttgc caatagcact ttatctttac gatcgatgga catcaaacat
     5161 cacgtcatat tatctctacg tgatcgatcc aaatcttatt caatgaaaat aaaattataa
     5221 acgatatgaa gcttattcgc ttcgggcaaa aaggcaaaga aaagccggga atattattgg
     5281 atggaaagta ccgggatgtt tcctcttaca ttcatgacta tgatgaaaca ttttttgaga
     5341 atgatggtct gcaaagactg gagcaaataa ttcattcatc aacaacaact atggcggaga
     5401 ttccgcagga tacaaggctt ggatctccgg ttgcaagacc atccaaaata gtatgtatag
     5461 gtctcaacta caagaatcat gtcatggaga ccaatgccaa catacccact caacctattt
     5521 tatttttcaa gtcaaccact gctttgagtg ggccgaatga cgatgtaatg ataccacgtg
     5581 acagtgttaa gacggattgg gaaatcgagt tggcggttat catgggacga cgtgcctcgt
     5641 atattgaacc ggaagaagca tatgactata tcgccggata tagcctaatt aatgattaca
     5701 gtgagcgtgc gttccaaaaa gagatgggag gccaattctg caagggaaaa agttgtgata
     5761 cctttgctcc aatgggccca tttttagcaa caaaggatga agtagaggat gtccataatc
     5821 tttctcttac gttaagcgta aatgagatca tcaggcaaca gggcagtaca gctgatatga
     5881 tattcgacat tccttacctt gtatcgtaca tcagcagatt catgacactt cttccgggtg
     5941 atattttgtg taccggcacc cctgagggtg taggtgcggg tatcaagcca gaaccggtat
     6001 tcttaaggtc aggggatgtt gtcaggatgt cgatagatga gcttggcagt tcaacgcaaa
     6061 gggtaatagc ttatagtggg ggttgagaac aatggagcat ctgaagtagg tagatgatta
     6121 atagccacaa tgcttgaagg tactgccttt cggggtagct ttgccaaaat tcagttgatt
     6181 tgctcgggaa ttaaattttt catttgcctc caaatccttg tgtatggcgg tttttgagtt
     6241 attactttcc tacct
//
LOCUS       DAOQMC010000138         6133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5523_length_6133_cov_4.940441, whole genome shotgun sequence.
ACCESSION   DAOQMC010000138 DAOQMC010000000
VERSION     DAOQMC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_6133_cov_4.940441"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKV31_06555"
     CDS             <1..349
                     /locus_tag="PKV31_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB92552.1"
                     /translation="SIQKEIEEATALELPMLEKNLVFLSTIASVATLLGLFGTVLGMI
                     RAFAGMATSGAPDASALATGISEALINTALGITTSAIAIVAYSYFTTRIDGMTYRIDE
                     AGYSMQQQFSSQY"
     gene            404..1048
                     /locus_tag="PKV31_06560"
     CDS             404..1048
                     /locus_tag="PKV31_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB92553.1"
                     /translation="MPKHKIARKSTAVDMTAMCDVSFLLLTFFMLATKFKPPDPVEVV
                     TPASISDTKVAETNLIMITIDKNGRVFFDMDGIPTKMDLINQINDNMRLGLTEEEKKN
                     FALSSGVGVPFSQLKALLKHSPTEQKSFNSTGIPVDTSLTDSNELFKWVAYGRLSNPE
                     ARLSVKGDGSVKYPVVKAVFSTLANKKIQGNKFYAVTSLKANPNKPVEEPEKTN"
     gene            1081..1650
                     /locus_tag="PKV31_06565"
     CDS             1081..1650
                     /locus_tag="PKV31_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB92554.1"
                     /translation="MAQMDTASSGGGNKKGGSKTKKLSTRVDLTPMVDLAFLLITFFM
                     LTTTMNKPQAMQINMPVERNPNDPPPPEVPAAKVLTLILGANNVIWYYEGLDSPEIKN
                     TDYTEIRNIILDKKKRVEATIKPDDKIKSVIVIIKPTDDSNYKNVIDMLDEMAIDEIG
                     TYTMVPVTPQELALVDKFKNGGGTPPPAN"
     gene            1725..2570
                     /locus_tag="PKV31_06570"
     CDS             1725..2570
                     /locus_tag="PKV31_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNB92555.1"
                     /translation="MKSEDILKADMLDIIFDGRNKEYGAYDLRRKYPKFLGRALIICI
                     LIVSAILGYALLKERFAGIFEKEVQVEANLADLEAPPPMEENEPPPPPETAPPPPPER
                     ASVQYVPPVVKEVVAQEAEIATVEELKDKDPGVKTVEGDPNAKVIIDTKADAAPGLFE
                     SSEKKVVAKVEDDNKIFTVVEQQPEFPGGIGAMYKWIGENLKYPSEARNNGLQGKVIL
                     QFTVEKDGSIANVKVVRDGVGGGAGDEAVRVVKKMPAWKPGKQNGKSVRVQYTLPVTF
                     KLEGE"
     gene            2654..2887
                     /locus_tag="PKV31_06575"
     CDS             2654..2887
                     /locus_tag="PKV31_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92556.1"
                     /translation="MTKPGNQINQSFQLLATVLALIIGIFFTGFGISILTGLIKMPND
                     DGPIMKIFGGLLVIYGIFRLIRVYFKYKSYREE"
     gene            2910..3830
                     /locus_tag="PKV31_06580"
     CDS             2910..3830
                     /locus_tag="PKV31_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNB92557.1"
                     /translation="MAGIILSLMIASCNQAPTTQYRDTPTSGEAHIAGDFLYKTMLDS
                     EIQIFTHEYQKASIAVDYMSEALAVKAFLSDSIDGIVIGRELNASETKTFRDKKKYDP
                     KTVRLATDAVAVIVHPDNKAEDLTIDLLRNILNGSTTDWGDGRKIEVLIDNEGSGIIT
                     FLRDSVMAGSSFGKNVYTLPQGTTMIDYVATHPNAMGFVGVNQITSFDGDANQVFNEK
                     IKPLAIGKVAGLKSYKPYQAYVASRNYPLLRFTNFIFNESHNGLTSGFATFMASDKGQ
                     RIILKDGLVPANAPIRLIQVNTSANDINSR"
     gene            3843..5468
                     /locus_tag="PKV31_06585"
     CDS             3843..5468
                     /locus_tag="PKV31_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92558.1"
                     /translation="MQKMMFRNFGLLGFLFLLISVANAQDIEEAYKKLSYGKTDEAVN
                     IAKSVANADPAIMANKFSLYSLLNAAERYDEAGKVLSEIKTSDESGPYGKTADVLMKL
                     DQGANPDDLTVEIDKAIRKGKKMKGFLYRTVGEYFLFNKKTNPVKAIQYIKMAIDDYG
                     LKNASTRMLLGDAYNLKNDAGNAVTNYEYAMEMDPTNAVPHYKIGTAYIRAKRYEFGV
                     PELRKAIETDPSYALAYKDLGKYYYDVDKYQEAKENYRKYMTMIGTPTLAEKVQYANI
                     LFLNKDYSEALPLLEQARVEDKANKYPNVLRMMGYAYHETHENNKAISALNNFLANRD
                     TTKILSQDYLYLGKIQAELGQDSLSRLNYLQAFEMDSTLDSEIKVIADTLFNQKKYDP
                     AGMLYMAIAKSTNLAADYFYATRADYLAGNYSRGIEAAEGIINKLPNEVEGYLWKARH
                     QALLDTPNKGAAAIDPYLQVVEKGKVDPSKYSKQMLDALNWLTVYYINIKADYAKAQE
                     YNDQALEVDANNEQVMKLFNFLLEATKPKSGKK"
     gene            complement(5573..>6133)
                     /locus_tag="PKV31_06590"
     CDS             complement(5573..>6133)
                     /locus_tag="PKV31_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB92559.1"
                     /translation="VIIKWLGSASSGLKLINADVKVLEHHFRQVQNTEAADRLAAFIR
                     MLQYLASDKQWHPLADVSPVKYSETEGMRMDDIYQYCMRHFKREISLDEIAGIAYMTP
                     QAFCRYFKKHTLKTFTEFVNELRINEACRLLVSDKHRTISSIAYQCGFNHAVTFNRVF
                     RDIMKMSPTEYLKNINAINKEEKLSK"
BASE COUNT         1956 a         1216 c         1341 g         1620 t
ORIGIN      
        1 gagcattcag aaagaaattg aagaagcaac tgcactggaa ctgcctatgc tggaaaagaa
       61 ccttgtgttc ttatctacca tcgcatcagt agcaacacta ctcggtttgt ttggtacggt
      121 attgggtatg attcgagcct ttgcaggtat ggcaaccagt ggagctccgg atgcatcagc
      181 acttgctacc ggtatttctg aggcattgat caatactgca ttggggatta ccacatcggc
      241 cattgcgata gttgcctata gctacttcac aacaagaatt gatggcatga cgtatcgcat
      301 cgacgaagcc ggctatagta tgcaacagca attcagctct cagtattgat agcgtattgg
      361 ttccggtttg atggacaatt ctttaacccc aaaaaattaa ataatgccta agcataaaat
      421 cgcaagaaaa agtaccgcag tggacatgac ggccatgtgc gatgtatcct ttctgctttt
      481 gacattcttt atgttggcga ccaaattcaa gccaccggat cctgttgagg ttgtcacccc
      541 cgcttctatt tcagatacca aggtggcgga gactaatctt atcatgatta ccatagataa
      601 gaatggaagg gtctttttcg atatggatgg tattccgacg aaaatggatt tgatcaatca
      661 gattaatgac aatatgaggc ttggtctgac cgaagaggaa aagaaaaact ttgccttgag
      721 ttctggtgtt ggagtgcctt ttagtcagtt gaaagcattg ttaaagcatt cgccaaccga
      781 gcagaaatca ttcaattcca ccgggattcc ggtcgataca agtctaacgg actccaatga
      841 attattcaaa tgggtcgcat atggaaggtt atctaatccg gaagcaagac tctccgtgaa
      901 aggtgatggt tcggtcaagt atccggttgt aaaggctgta ttttctaccc tggctaacaa
      961 aaaaattcaa ggaaataagt tttatgctgt taccagcctg aaagccaatc ctaataagcc
     1021 ggttgaagag cctgaaaaaa caaattaaat tattaatctg tgtaaaataa ccaaattgtt
     1081 atggcacaaa tggataccgc ctcttccggc ggaggaaata agaaaggcgg cagtaaaacc
     1141 aaaaagttat caacccgcgt tgaccttacg ccaatggtgg acctggcttt cctattgatt
     1201 acattcttca tgctcaccac aacaatgaat aaacctcagg ctatgcaaat caatatgccg
     1261 gtggaaagga atcccaatga tcccccaccc ccggaagtac ctgcagctaa ggttcttaca
     1321 ttgatactgg gagcaaataa tgtcatttgg tactatgagg gactggatag tccggaaata
     1381 aaaaatactg attatactga aatcagaaat attattctgg acaaaaagaa aagagtggag
     1441 gctacgataa aaccggatga caaaatcaaa tctgtaattg taatcatcaa accaactgat
     1501 gactccaatt ataaaaatgt aatcgatatg ctggatgaaa tggcgattga tgaaataggt
     1561 acttatacga tggtacctgt gacacctcaa gagttggccc tggtggataa atttaaaaat
     1621 ggaggtggca cgccgcctcc ggctaattaa aaatataacg taaaaaatag ttgtaaaatg
     1681 aaattatata acttaatttt gcgttataca aataaaaagg cgtcatgaaa agtgaagata
     1741 ttttaaaagc ggatatgctc gacatcattt ttgatggtcg aaataaagaa tatggagctt
     1801 atgatctgag gcgaaagtat cctaagtttt tgggtcgggc cctcatcatc tgcatattga
     1861 ttgtttctgc aattttagga tatgcccttt tgaaagaaag gtttgcagga atatttgaaa
     1921 aagaggtaca agtagaggcc aatctggctg atcttgaagc acctcctcct atggaagaaa
     1981 atgagccacc cccacctccg gaaactgcac ctcctccacc accggaaaga gcttcggttc
     2041 agtatgtgcc accggtcgtt aaagaggtag ttgctcagga agcggaaata gccacagtcg
     2101 aagaattgaa ggacaaggac cccggtgtaa aaacagttga gggtgacccg aatgccaaag
     2161 tgattattga cacaaaggct gatgctgcac ccggattatt cgaatcttcc gaaaaaaagg
     2221 ttgtagctaa agtagaagat gataacaaaa tcttcaccgt ggtggaacaa caaccggaat
     2281 tcccaggtgg gatcggagct atgtacaagt ggatcggaga aaacctgaag tacccatcag
     2341 aggcacgtaa taacggtttg caaggtaagg ttatactcca gtttacagtt gaaaaagacg
     2401 gttccatagc caatgtaaaa gttgtacgtg atggcgtagg tggcggtgcc ggcgatgaag
     2461 ctgtcagggt agtgaaaaaa atgccagcct ggaaaccagg aaaacaaaat ggtaagtctg
     2521 ttcgcgtaca atatacgtta ccggtgactt tcaagttgga gggagaataa cattcgcgtt
     2581 aaaattttta taagaaaata gcaatatgga attcttccct gttgctattt tgtttttacg
     2641 attaattgac aaattgacta aaccaggaaa tcagatcaat caatcttttc agctattggc
     2701 caccgtgctg gcattgataa tcggcatatt ctttaccggt ttcggtataa gcatcctgac
     2761 cggtttaatc aagatgccta acgatgacgg tccaatcatg aagatattcg gaggtctgct
     2821 ggtgatttac gggattttca ggttgatcag ggtatatttc aaatataaat cctatcggga
     2881 agagtaacat gaaggtaaaa attggttcag tggccggaat catcctttcg ctaatgattg
     2941 catcatgtaa tcaggcacca accacgcaat acagagacac ccctacctca ggagaagcac
     3001 atattgccgg tgacttcctg tataagacca tgctagatag tgaaattcaa atttttaccc
     3061 atgaatacca aaaagcatcc attgctgttg actatatgtc tgaagctcta gcagtcaaag
     3121 ccttcctgag tgacagtatc gatggaattg taataggaag ggaattgaat gcttctgaga
     3181 caaaaacatt cagagataag aaaaaatatg atcccaagac tgtccgtctt gccactgatg
     3241 ctgtagcggt gatcgtccat ccggacaata aggctgaaga tctcaccatc gacctcctta
     3301 gaaatatatt gaatggttct acaaccgatt ggggtgacgg ccgtaagata gaagtattga
     3361 tagataatga agggagtggt atcattactt ttctgagaga ttcggtaatg gccggaagct
     3421 ccttcggtaa aaatgtatat actttgcccc aaggtaccac catgatcgat tatgtggcaa
     3481 ctcatcctaa tgctatgggt tttgtcggtg tcaaccaaat caccagtttc gatggtgatg
     3541 ccaatcaggt tttcaacgaa aagataaaac cacttgctat tggtaaagtc gccggtctaa
     3601 agagctataa accataccag gcctatgtgg catcccgaaa ttaccccttg ttgagattta
     3661 ctaattttat tttcaatgaa tcacacaatg gactaaccag tggatttgct acctttatgg
     3721 catccgataa ggggcaacgt attatcctta aggatggact ggtacctgct aatgcgccaa
     3781 tacgtctgat tcaggtaaat acttcggcta atgatattaa ttccagataa aatcaattaa
     3841 caatgcaaaa aatgatgttt agaaattttg gtttgcttgg cttcctattc ctgttaataa
     3901 gtgtagcgaa tgctcaggac atcgaggagg cctataaaaa actatcttat ggcaaaactg
     3961 atgaggcggt gaatattgct aaatcagtcg ccaatgctga cccggctatt atggcaaata
     4021 aattttcctt atatagtctt ctgaatgcag cagagagata tgatgaagcc ggcaaggtgc
     4081 tttcagaaat taaaacaagt gatgaaagtg gcccttacgg taagactgcc gatgtcttaa
     4141 tgaaattgga tcaaggagcc aatcctgatg acctgactgt tgagatcgac aaggcaatca
     4201 gaaagggtaa gaagatgaag ggattccttt atcgtactgt cggcgaatat tttcttttta
     4261 acaaaaaaac caatccggtg aaggcaattc aatacatcaa gatggccatt gatgattatg
     4321 gattgaagaa tgcatcaacc agaatgcttt taggcgatgc ctataacctg aaaaatgatg
     4381 cgggaaatgc agtaaccaac tatgaatatg ccatggaaat ggaccctacc aatgctgtcc
     4441 ctcactataa aatcggaact gcctacatca gagcaaaaag atatgaattt ggcgtgccgg
     4501 aactgagaaa ggctattgag acagatccca gttatgctct ggcatacaag gatttgggta
     4561 aatactacta tgatgtcgat aaataccagg aagctaaaga aaattatagg aaatacatga
     4621 caatgatcgg gactcctact ttggcagaaa aagtacaata cgccaacatt ctattcctca
     4681 acaaagatta cagcgaggca ttgccgctgc ttgaacaggc ccgtgtggaa gataaggcca
     4741 acaaatatcc caatgtcctc agaatgatgg gatatgcgta tcatgagact catgaaaata
     4801 acaaagctat cagcgcgtta aataactttc ttgcaaaccg ggatacaacc aagatattga
     4861 gtcaggacta tctgtatctc ggaaagatcc aggcagaatt aggtcaggat agtctttccc
     4921 ggctgaatta tctccaggcc tttgaaatgg actcaacgct tgactccgaa atcaaggtta
     4981 ttgcagacac attgttcaat cagaaaaaat atgacccggc aggcatgctg tatatggcta
     5041 tcgccaaatc aaccaatctg gcagccgatt atttctatgc aaccagggca gattatctgg
     5101 ccggtaatta ttcccgcgga atagaagcag ctgaaggaat catcaataaa ctgcctaatg
     5161 aggtcgaggg atatctgtgg aaagccagac atcaagcttt attggataca ccaaataaag
     5221 gtgctgcagc catcgatcct tacctgcaag tggtggaaaa gggaaaggtc gatccatcca
     5281 aatacagtaa acaaatgctg gacgccctca actggctgac cgtatattat atcaatatca
     5341 aggcagatta tgcaaaagca caggagtaca atgaccaggc tcttgaagtc gatgcaaata
     5401 acgaacaagt aatgaagttg ttcaacttcc tgcttgaagc aacaaagcct aaatcaggca
     5461 agaaataata agacgaaaaa tatttgattt tcgaagaata aaaaaggcgg cccggggtcg
     5521 ccttttttat tcgctggccg tcaagaatca cccgccatcc atctccaaat ccttatttgc
     5581 ttagtttctc ctccttgttg atggcattta tatttttaag atattccgtc ggcgacatct
     5641 tcataatatc gcgaaagacc cgattgaagg tcactgcatg attgaatcca cattggtagg
     5701 caatacttga aatagtacga tgtttgtcag ataccagcaa cctgcatgct tcatttattc
     5761 tgagttcatt gacaaactcc gtaaaagttt ttagtgtgtg tttcttgaaa tagcggcaga
     5821 atgcctgagg ggtcatgtat gcaatcccgg cgatttcatc caatgatatt tcacgtttga
     5881 aatgtcgcat acagtattgg tagatatcat ccatacgcat tccctctgtc tccgaatatt
     5941 tgacaggact tacatcggcg agtgggtgcc attgcttgtc actggccaga tattgcaaca
     6001 tccggataaa ggcagcaagc ctatcggcag cttctgtatt ttgtacctgc ctaaaatgat
     6061 gctccaacac ttttacatca gcattgatca acttcagccc ggaggatgcg gatccaagcc
     6121 acttgatgat tac
//
LOCUS       DAOQMC010000139         6124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5530_length_6124_cov_5.069863, whole genome shotgun sequence.
ACCESSION   DAOQMC010000139 DAOQMC010000000
VERSION     DAOQMC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5530_length_6124_cov_5.069863"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKV31_06595"
     CDS             complement(<1..269)
                     /locus_tag="PKV31_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNB92560.1"
                     /translation="MKQKTYEVLNRYSVEEFKRLKKLPVVIVLDNLRSGLNIGSAFRT
                     ADAFAVSSIELCGICAIPPNREILKTALGATESVQWRYHDSTKDC"
     gene            complement(277..1899)
                     /locus_tag="PKV31_06600"
     CDS             complement(277..1899)
                     /locus_tag="PKV31_06600"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNB92561.1"
                     /translation="MQTKFIFVTGGVTSSLGKGIISSSLAKLLQARNLRVTIQKFDPY
                     INVDPGTMNPYEHGECYVTDDGAETDLDLGHYERFLNIRTSQANNVTTGKIYQTVIKQ
                     ERAGKYLGKTVQVIPHITDEIKRRIRLLGESGEYDVVITEIGGTVGDIESLPYIEALR
                     QLRWDLGMQNSLVIHLTLIPFLSAAQELKTKPTQHSVKELQSLGIQPDILVCRTERPI
                     SQEIKQKLALFCNVEQNSVIESIDASSIYEVPLLMEKEGLDAIVLKKFNLQGLPEPNL
                     DKWKAFLSTLKYPKKEVKIALVGKYIELKDAYKSIYEAFIHAGAANECKVHVEPVHSE
                     DLLDSEVCQKVLSKMDAVLVAPGFGLRGIEGKLNAIRYVRENKIPFFGICLGMQCAVV
                     EYAKNIAGMKDAASTEVDPNTPYPVIALMEDQQHLKKKGGTMRLGAYSCKITDKSHAM
                     KAYGSALIEERHRHRWELNNQFRDKLKNAGLKITGVNPDRDLVEIIEIEDHPWFVGVQ
                     FHPELKSTVEIPHPLFVSFVEAALTHQAQNMA"
     gene            2558..4093
                     /locus_tag="PKV31_06605"
     CDS             2558..4093
                     /locus_tag="PKV31_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNB92562.1"
                     /translation="MPKLLNSAVKVYLKLVHGMLERSITAPIEAQEKVFNKLIFNFKN
                     TKYGKLNGIKHVKNYKSYHKAYPVVTYDHLKPFIERMMKGESDVLIGGNVRFFSKSSG
                     TTNDKSKFIPVTRANLYKNHLKSAWDSLSLFYRKRPDAELFNKKSLMISGSLSHFPEY
                     PKAIVGDISAIMTKNMPTIARSFYTPDLEIAMLSDWNEKIARITEQVGSQDVVMFGGV
                     PTWLLVTFRELLKKQNKENLLEIWPNLQGYLHGGVGFSPYVEQFKALIPSKDFIYQEI
                     YNASEGFFGIQQHNSDEDMLLLVNNSVFYEFLPMSEWEKDSPEAIPLKDVETGKPYAI
                     LITSNNGLARYMMGDVVEFSSTCPYKFKIKGRTQHYINIFGEEVMVDNTDKALAMTCQ
                     ATGSSIDNYTVGPIYLTTAMKGGHEWIVEFNKKPEDINAFGQLLDNNLRTLNSDYDAK
                     RSYDLALMPLKLTIVPVGTFHRWLQSRNKLGGQNKVPRLANHRNYIDALLEFGDSLAK
                     TIT"
     gene            4099..4398
                     /locus_tag="PKV31_06610"
     CDS             4099..4398
                     /locus_tag="PKV31_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92563.1"
                     /translation="MDDLNSLTSLELPVTLPDTQVGYEEMLNAIADVVNRMLNDNPDL
                     LFSYLYRLDVDEAKIKFALSPLAETAPHIGLANLIIERQKQRIRTRQEYSEKAKK"
     gene            4411..5139
                     /gene="recO"
                     /locus_tag="PKV31_06615"
     CDS             4411..5139
                     /gene="recO"
                     /locus_tag="PKV31_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNB92564.1"
                     /translation="MSKLLKDKGIVVRTFKYGETSVILDILTEVHGLGSFILQGVRKS
                     KSRTSPSSIQLAASVDLDFYMKENTDLHQLKELKVNTIRTSIYSDIRKIALCMFLSEL
                     TRRMLNKYENHQLEFSLLQSVLDDLEANDDNWSIHIWYLIRLLEISGFSLLKHRVHPE
                     YYYDIPSDTITSNRPLHHSYLSGYDMITLTQLNQADVRQVSQFKPKRDERNRLVEKLL
                     DFLKVHISGFQHIQSFDVLKSVFE"
     gene            5299..6081
                     /locus_tag="PKV31_06620"
     CDS             5299..6081
                     /locus_tag="PKV31_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB92565.1"
                     /translation="MLHKVTTFLSPTLGRSVKIHILETKKAIGNECLTILFNDGQDFA
                     ALRISDRIAGFELDYPNVPLRIIGLEAGDRLHEYGTMYKNDYKNRGNKAGKYNQFINN
                     ELLPYLFSRHKISLLSKMTGFAGFSLGGLSAMDIVLHNPDVFSFSGVFSGSFWWRHSP
                     FTEEDPDGSRILHEYIDTHAISSKQHFWFQTGALDEESDRNRNGIIDSIDDTVDVMLC
                     LERKGYKQGKNMTYLEVSDGTHDTGTWHRVMPDFLEWVVKSL"
BASE COUNT         1872 a         1251 c         1191 g         1810 t
ORIGIN      
        1 atacaatcct tggtcgaatc atgatatcgc cattgaacag attccgtagc tcccaaagct
       61 gttttcagga tttctctatt cggtggaatg gcgcagattc cacacaattc aatggatgag
      121 acagcgaaag catcagcagt cctgaatgct gagccaatat ttaatccgga acgcagattg
      181 tccagaacga ttacaacagg taatttcttc aatcttttaa attcctcgac tgaatatcgg
      241 ttcagaactt catatgtttt ctgcttcatc ctcgggtcaa gccatatttt gagcctgatg
      301 tgtcaaggct gcctcaacaa aactgacaaa taatggatgt ggaatctcaa cggtcgattt
      361 caactccgga tgaaactgaa caccaacaaa ccaaggatga tcctcaattt caataatctc
      421 taccaggtcc cgatccggat taacccctgt aatctttagt cctgcatttt tcaatttatc
      481 ccggaattgg ttgtttaatt cccatcgatg acggtgtctt tcttcaatca aagcagatcc
      541 ataggccttc atagcatgtg atttgtcagt gattttacag gagtacgccc ctaatctcat
      601 ggtcccgcct ttcttcttaa gatgctgttg atcctccatc aaagcaatca cagggtatgg
      661 tgtattcggg tcaacttcag tagatgcggc atctttcatt cctgcgatat tcttggcata
      721 ctccaccact gcgcattgca taccaaggca gataccgaaa aacgggattt tattttccct
      781 gacataacga atggcgttca atttgccttc aatgcccctc aacccaaaac cgggagccac
      841 cagcacggca tccatcttac tcaatacctt ttggcatact tctgaatcta aaagatcctc
      901 tgagtggaca ggttctacat ggaccttaca ttcattggca gcaccggcat ggataaaagc
      961 ctcataaata gatttatatg cgtcttttag ctcgatatat ttacctacga gtgcaatttt
     1021 aacctctttc ttaggatatt tcaatgttga caggaacgct ttccatttgt ccaggttagg
     1081 ttctggcagt ccttgaaggt tgaatttttt taaaacaata gcatccaggc cttccttttc
     1141 catcagcaat ggcacctcat aaatagaaga agcatcgatc gattctatca cagaattctg
     1201 ttcaacatta cagaataaag ctaatttttg tttaatttcc tgagaaattg gcctttctgt
     1261 acggcaaaca aggatgtccg gctgaatccc aagcgactgg agctccttta ctgaatgttg
     1321 tgtaggcttg gttttcaatt cttgagctgc acttagaaaa gggataagtg tcaggtggat
     1381 aaccagcgaa ttctgcatgc ccaaatccca acgcaattgt cgcagtgcct cgatataagg
     1441 caaggactca atatccccta cagttccccc tatttcggta ataacgacat catattctcc
     1501 ggactcaccc aacaatctga tacgtcgttt gatttcatct gtaatatgag gtatgacctg
     1561 aactgttttg ccaagatatt tacctgcccg ttcctgcttg attacggtct gatatatttt
     1621 accggtagtt acattattcg cctgacttgt cctgatattg agaaatcgtt catagtgacc
     1681 cagatcaaga tcagtctctg ccccatcatc ggttacgtaa cattcaccgt gttcatatgg
     1741 attcatagta ccgggatcaa cgttgatgta tgggtcgaac ttttgaatag taacccttag
     1801 atttcgtgct tgaagtaatt ttgcaagaga ggaggaaata atgcctttcc caagagaaga
     1861 agtaactcca cccgtaacaa agataaattt agtctgcata ttcacttgtc gttacggact
     1921 gtaaagatat ggtaaaatac ggaattcagg aggaatattt ctttggaaat aaataaagat
     1981 ttggggattt agggaagatt ggggtgggtg ggtaatttta agtaggtaga taactaatat
     2041 tcatccttga gctggaacaa gaaatctcag attccattta ataaacttaa gtcgagtacg
     2101 ggttgaaaaa aaattcagat tgtttaaaaa tgaggatatg gtaaatgtct cacgtattaa
     2161 tcaactacct aattagattt ggacttccca tttcattcga tttttaatcc ataatagcat
     2221 tcagggcact gaccgaaatt ggttctcacc agctgtaaat taagtaggta gataagtaat
     2281 actaaaaggg ggccacctta taaggcaatc aggatttaaa ttctgatatt tagtcaatta
     2341 attccaaaaa aaacaatttc actttaagcg aagccaatat ctatagataa attgggtatt
     2401 atccaactac ctaattgttt ttttcctcag caacactcct gaatactaaa atcagattaa
     2461 ttgaccctaa gtgcatgatc caatggactt tctccaaaaa atttgaacaa tttttttcaa
     2521 aataccttat atttgagact gtttgtaact gaatcctatg ccaaaattgt taaacagtgc
     2581 tgtaaaggtt tacttgaaac tggtacacgg aatgttggag cgatccatca ctgcaccaat
     2641 agaagcacag gaaaaagtgt ttaacaaact gattttcaac ttcaaaaaca ctaaatacgg
     2701 aaagcttaac ggcattaagc atgtcaaaaa ttataaatca taccataagg cctatccggt
     2761 tgtaacgtat gaccatctca aacctttcat agaacgcatg atgaagggag aaagtgatgt
     2821 acttatcggt ggcaatgttc gctttttttc gaaatcatca ggtaccacca acgacaaaag
     2881 caaatttatt ccggtaacca gggctaattt gtacaaaaat catctaaaga gtgcatggga
     2941 tagcttgtcc ctattctacc gaaaaaggcc tgatgcggaa ttgttcaaca aaaaatctct
     3001 aatgattagc ggctccttga gtcactttcc ggaatacccc aaagctattg taggtgatat
     3061 ttcagcaata atgacgaaga acatgcctac tattgcccgt tctttctaca caccggacct
     3121 ggaaatcgct atgctatcgg actggaacga aaaaattgcc cggataaccg agcaggtcgg
     3181 tagtcaggat gtagtaatgt ttggaggagt ccctacctgg cttttagtga cttttcggga
     3241 attgcttaaa aaacaaaata aggagaacct tttagaaatt tggcccaatc tccaggggta
     3301 tcttcatggc ggagtcggtt tcagccctta tgtcgaacag tttaaggctt tgattccttc
     3361 aaaagatttt atctatcagg agatttacaa tgccagcgaa ggctttttcg gcatccaaca
     3421 acacaatagt gatgaagaca tgcttctgct tgtcaacaac agcgttttct atgaatttct
     3481 tccaatgagt gagtgggaaa aagattcccc ggaagcaatt cctctcaagg acgtagaaac
     3541 aggaaagcct tatgccattt tgataacttc caataacgga ctggcgagat acatgatggg
     3601 agatgtcgtg gagttcagtt cgacctgccc ctataaattc aaaataaaag gtcgtaccca
     3661 gcattatatc aatattttcg gagaagaagt aatggtggat aatacagata aggcattggc
     3721 gatgacttgt caggctaccg gatcaagcat cgataactat actgttggcc caatatatct
     3781 aactactgct atgaaaggcg ggcatgaatg gattgtcgag ttcaacaaaa aaccggagga
     3841 catcaatgct ttcggtcagt tgctggacaa taatcttcgc actcttaaca gtgattatga
     3901 tgccaaacga tcttatgatc tggctttgat gccgcttaaa cttactattg tacctgttgg
     3961 cactttccat cggtggcttc agtcccgtaa taaattgggg ggacagaata aagtccccag
     4021 actggccaat catcgtaatt atattgatgc tttgcttgaa tttggtgaca gtcttgcaaa
     4081 aaccataacc taaggttgat ggatgatctg aattctctta cttcgttgga actaccggta
     4141 acattaccgg acactcaggt tggttatgaa gagatgctca atgccattgc tgatgttgtc
     4201 aaccggatgt tgaatgacaa tccggatttg cttttcagtt atctttatcg attggatgta
     4261 gatgaagcga aaatcaaatt tgccttgtca cctttagcag aaaccgctcc tcatattgga
     4321 ttggccaatc taatcataga acgacagaag caacgaatcc gaacccgaca agaatattcc
     4381 gaaaaagcaa aaaaataaat ttaatgtgcg atgagcaaat tgctgaaaga taaaggcatc
     4441 gtcgtgcgta ccttcaaata tggtgaaacc tccgttatac tggacattct tacagaagta
     4501 cacggtcttg gatccttcat cctccaagga gtccgaaagt ccaaaagcag gacatccccc
     4561 tcatccatcc aattagccgc atcggtcgac cttgatttct acatgaagga aaataccgat
     4621 ctgcatcaat tgaaggagtt aaaggtcaat actattcgta cttcaattta cagtgatatc
     4681 cgaaaaattg cgttatgcat gtttctttca gagcttacga gacggatgct gaacaaatat
     4741 gaaaatcatc aattggagtt ttccttgctc cagtctgtat tagatgattt ggaggcaaat
     4801 gatgacaact ggtcgattca tatttggtac ttgattcgtt tgcttgaaat atccggattt
     4861 tcattattaa aacaccgtgt tcatccggaa tattactacg acattccctc tgacacgatc
     4921 acatctaata ggcccctgca ccattcgtat ctttccggat atgatatgat taccttgaca
     4981 caacttaatc aagccgatgt caggcaggta tcccaattta agcccaaaag ggatgaacgt
     5041 aatcgtttgg tagaaaaatt gctggatttc ctaaaagtcc atatttcggg atttcagcat
     5101 attcaaagct ttgatgtgtt aaaatccgtt tttgaataac tccttaaaca aaaattaaat
     5161 atttcggaag aaattgaaag tattcattca ttctaatcaa tttataaaac ggcatattaa
     5221 atgaccggct aatctgtaat tattttttat ttttgtgaaa acatataacg accttcatat
     5281 tgacttgata aaaaaataat gcttcacaaa gttaccactt ttttatcccc tacattgggc
     5341 cgatcagtta aaatccatat attggagacc aagaaagcaa ttggtaatga atgccttacg
     5401 attctattta atgacgggca ggattttgct gcacttagaa tttcagatcg aatagccgga
     5461 ttcgaactgg attatccgaa tgtccccctg cgtatcattg gattagaggc cggtgatcgc
     5521 ctccatgaat atggtactat gtataaaaat gattataaga acaggggcaa caaggcgggt
     5581 aaatacaatc aattcatcaa taatgaactc cttccctatc ttttttcacg acataaaatc
     5641 tctttgttgt ccaaaatgac tggatttgcc ggattctcac taggaggtct ttctgctatg
     5701 gatattgtct tacacaatcc ggatgtattc tctttttccg gtgtcttctc agggagtttt
     5761 tggtggcgtc actcaccatt taccgaagaa gatcccgacg ggtccagaat tctgcatgaa
     5821 tatattgaca cccatgcaat cagtagcaag caacattttt ggtttcagac aggtgctttg
     5881 gacgaagaaa gcgatcgcaa cagaaatggc attattgact caattgatga cacggtcgat
     5941 gtaatgcttt gcctggaaag aaaaggttac aaacagggca agaatatgac ttatcttgaa
     6001 gtaagtgacg gcacgcacga caccggaact tggcacagag tcatgcccga ttttctggag
     6061 tgggtagtta aatctcttta aacatcccgg gaaaggcaga atgcataatt gaccttataa
     6121 aaac
//
LOCUS       DAOQMC010000140         6117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5550_length_6117_cov_4.745299, whole genome shotgun sequence.
ACCESSION   DAOQMC010000140 DAOQMC010000000
VERSION     DAOQMC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5550_length_6117_cov_4.745299"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /locus_tag="PKV31_06625"
     CDS             <1..1905
                     /locus_tag="PKV31_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNB92566.1"
                     /translation="EPSYMSDQIKHECGIALIRLRKPLEFYREKYGTALYGINKLSLL
                     LQKQRNRGQDGAGIATIKLNPEPGTRYISRKRSNSANYLNDLLKSVYKYFNNLPEERL
                     NDPAWLKANRPYMGEVLMGHLRYGTHGLNSIETCHPFMRQNNWMSRTLVLAGNFNMTN
                     TDELFQELIELGQFPKEKSDTVTMLEKIGHFLDNEVQNLFDHFKRIGYSNQEITEQMK
                     SSLDVNNVIRKAFRKVDGGYAIMGMIGTGEAFVVRDPHGIRPAYYYMDEDVIAVASER
                     PALQTAFGVRYKNVHEVPPGHTMVIRTNGEVEFQPFTDPAPRKSCSFENIYFSRGNDR
                     KIYLERKRLGEQLAKPIMEMVNYDFDNTVFAFIPNTAETAFYGLVEGLEKELNEVKKK
                     KILDLGASADATSLDQILSTRVRTEKLVVKDAKIRTFISDDSSRGDLMSHVYDVTYGI
                     VKNDRDTLVLLDDSIVRGTTLKQSIINILSKLFPKKIIIVSSAPQIRYPDCYGIDMSV
                     MKSFVAFQAVIEMLEESGREELIEEVYHKCKEQEHLPKEMMTNHVKSLYDVFTDKEIS
                     ERIARIVRPDNLPYDLEILFQSIDGLHAACSEEVGDWYFSGDYPTPGGTKVVNQAFIN
                     YMEKSTERAY"
     gene            1912..3147
                     /locus_tag="PKV31_06630"
     CDS             1912..3147
                     /locus_tag="PKV31_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB92567.1"
                     /translation="MKILVAGGGASGFMAAITAAERWPHHTVIIAEKSHRVLSKVLIS
                     GGGRCNVTHRPLDIHDFSDQYPRGKRFLKKVLHEFGAADAIQWFESRGVRLKTEVDGR
                     VFPASDDSASIAGCLEGEARRLGIQVLLKKGLDSFEKVDSGFQVHFSDGTKEVFDRII
                     LATGGFPKSSSYDFLREAGIAIKEPLPSLFTFNSPGNPISKLMGVSVPFAQVKIGGTK
                     LVHHGPLLITHWGFSGPAVLKLSAFGAELLAEMKYEFNVEIRWCNDITREECRAMWLG
                     WRDKKVKTLIANHSGLNLPERLWNFLVEEAQISDKTRWADIGNKGIDSLLNQVFSNIH
                     AIKGKTTFKEEFVTCGGVCLDKVDHRTMEYKNIPGLYFAGELLDVDGVTGGFNFQNAW
                     TTGFIAGRSVGQNYSSVRL"
     gene            3785..4669
                     /locus_tag="PKV31_06635"
     CDS             3785..4669
                     /locus_tag="PKV31_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB92568.1"
                     /translation="MSKTLRNIPLFWFSMLGYFAFVEIIVLWRDPQLFLSGQIMMNRL
                     IYIIIFGLLLILQRKLPVKQFLFFSILAAYAALTLLYKETALLNRLFYPVLDPYLSEL
                     DFQLTGIQPALVFSERFDSVFFSELMFLGYFSYYLTPLAILILIFFKKFELMEQFGFV
                     LITSFLLYYLVFIFIPAIGPQFYFSYPDNFVEAKGLFGHLVKLIQENGEAPTAAFPSS
                     HVGVALICMIWLWRNAKKYVYYFLPNVFLLILATVYIKAHYLVDVLAGAITAPLVFFL
                     VVKLYDQLKYRSTGSLPQ"
     gene            4706..5830
                     /locus_tag="PKV31_06640"
     CDS             4706..5830
                     /locus_tag="PKV31_06640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB92569.1"
                     /translation="MSIEILEVKNKSDLKSFIYLPEKIHRNHANWVHPIYLDEKQFFN
                     FDKNEAFRHNRTALFLARENGRVVGRIMAIIPDEYNNMKNVRTARFCYFEAPENKRIF
                     DALLNKVEQWAKDAGCNLMVGPMGFSDKEPQGFLTAGYNEKTMIVTNCSFRYMKEFLE
                     ARDYSPFVELCQYDVPLSNQIIQRYRALADRVESNMRVTVHEFTKTSEIKPFVQSVFR
                     LINTTYTDIYGFTKVTEKEAEDFANRFIPLLNPRLIKLITDESGEVVAFVIGMPDLSG
                     AIKKSRGRIFPFGWFHILRAFRNSKRMVLLLGAVKNEMQNKGLDAVLAVRLIRSALDL
                     GFDIMDSHLIMRKNIKMRREIERLENHRMYKEYTIYSKEL"
BASE COUNT         1751 a         1128 c         1448 g         1790 t
ORIGIN      
        1 gaaccttctt acatgagcga ccaaatcaag catgagtgtg ggattgcact tatccgcctc
       61 cgaaagccat tggaatttta ccgggaaaag tatgggacag cactttacgg catcaataag
      121 ttgagcctgt tgctgcaaaa acaaagaaac agaggccagg acggtgcagg aatagcaacc
      181 ataaaactca atcctgaacc gggcaccagg tatatttcac gcaaaaggag caacagcgcc
      241 aattacctga atgatctgct caaaagtgtt tataagtatt tcaataacct gccggaagag
      301 cgtcttaacg atccggcctg gctgaaagcc aataggcctt atatgggtga ggtcctcatg
      361 gggcacttac gctatggaac ccacggcctg aattccattg aaacttgcca tccatttatg
      421 cggcaaaata actggatgag ccgtacgctt gtactggccg gaaatttcaa tatgaccaat
      481 acggacgaat tatttcagga gttgatcgag ttgggccagt ttcccaagga aaagtccgac
      541 acggttacaa tgcttgaaaa gattgggcat ttcctggata atgaggtaca gaatcttttt
      601 gatcatttta agcgcattgg ttattccaat caggaaatca ccgagcaaat gaagagttca
      661 ctggatgtca ataatgttat ccgtaaagcc ttcagaaagg tggatggcgg ttatgctatt
      721 atgggtatga tcggcaccgg agaggccttt gtggttagag accctcacgg tattcgtccg
      781 gcttattatt atatggatga agatgtcatt gccgtagcaa gcgaacgacc tgccttgcag
      841 actgcctttg gtgtccgtta taagaatgtt cacgaagtgc caccaggcca taccatggtc
      901 atcaggacaa acggagaggt ggaattccag ccatttactg atccggctcc acgaaaatcc
      961 tgttcttttg agaatattta tttttcaaga ggaaacgacc gtaaaatcta ccttgagaga
     1021 aagcgcctgg gtgaacagtt ggccaaacca atcatggaaa tggttaatta tgactttgat
     1081 aataccgttt ttgctttcat tccaaatact gccgagacag ctttctacgg acttgtcgaa
     1141 ggcctggaaa aagagctgaa tgaggtcaaa aagaaaaaaa ttcttgatct gggggcaagc
     1201 gcagatgcga catcactgga tcaaatcctg tctacaaggg tgcgaaccga gaagttggtg
     1261 gtcaaagatg caaaaatcag aacctttatc tctgacgatt cgagcagggg cgacctgatg
     1321 tctcatgtct atgatgtgac atatgggatt gttaagaatg atcgggatac gcttgtttta
     1381 ctggatgact ccatcgtacg gggaacaaca cttaaacaat ccatcatcaa tatcctgtcc
     1441 aaattattcc cgaaaaaaat aataattgtt tcctcggcgc ctcaaatcag gtatccggac
     1501 tgttatggta tcgacatgag cgtgatgaag tcatttgtgg cgttccaggc ggtaatagag
     1561 atgttggaag aaagcgggcg agaagaactt atcgaggaag tgtatcacaa gtgcaaggag
     1621 caggaacatc tgccgaaaga aatgatgacc aatcatgtca aatcattata tgatgtcttt
     1681 acggacaagg agatcagtga aagaattgca agaatagtaa gacctgacaa ccttccatat
     1741 gatttggaaa tcctgtttca gtccatcgat ggcttacatg ctgcatgctc agaggaagta
     1801 ggggactggt atttttcggg agactatcca acacccggcg gtactaaggt ggtcaatcag
     1861 gcctttatca attatatgga aaagagtacc gagcgggctt attaatttct gatgaagatt
     1921 cttgttgcag gtggaggagc ctccggtttt atggcggcta ttactgcggc agagcgatgg
     1981 ccccatcata cggtcatcat tgccgaaaaa tcacatcgtg tactctccaa agtattgatt
     2041 tcaggtggtg gtcgatgcaa tgtaactcat cgaccgttgg acattcatga tttttcggat
     2101 cagtatccaa gaggaaaaag gtttttaaag aaggttctcc atgaatttgg tgcggctgat
     2161 gctattcaat ggtttgaatc acggggcgtc cggctgaaga cggaggtcga tgggagagtt
     2221 ttcccggctt ctgatgattc agcctcaatt gccggctgcc tggaaggaga agcaagaagg
     2281 ctgggaatac aggtattact caaaaaagga ttggattctt ttgagaaggt cgattccggt
     2341 tttcaggttc atttttcgga tggtacaaaa gaagtttttg atcgtattat cctcgctaca
     2401 ggaggctttc ctaaatcgtc ctcctacgat tttctccggg aggccgggat tgcaatcaag
     2461 gaacctctac caagcctctt taccttcaat agtccgggta atccgatctc caaattgatg
     2521 ggcgtatctg tgccgtttgc acaggtaaag attggaggaa caaaactggt tcatcacggt
     2581 ccgctcctta taacacattg gggattcagt gggcctgccg tgctgaaatt atcagccttt
     2641 ggtgcagaat tgcttgccga aatgaagtat gaattcaatg ttgaaatccg gtggtgcaat
     2701 gacattaccc gggaagagtg ccgggccatg tggttaggat ggcgggataa gaaggtaaag
     2761 acattgatcg caaatcattc gggattgaac cttccggaac gactgtggaa cttcctggtg
     2821 gaagaagctc agatttcaga caagacacga tgggcagata tcggtaataa aggaattgat
     2881 tcccttctca atcaagtttt ctcaaacatt catgctatca agggaaagac tacttttaag
     2941 gaggaatttg tcacttgtgg cggtgtttgc ctggacaagg tcgatcatcg caccatggaa
     3001 tacaagaaca taccaggttt atattttgca ggtgaattat tggatgtgga cggagtaacc
     3061 ggcgggttta actttcaaaa tgcctggacg acaggtttca ttgccggtag aagtgttggg
     3121 cagaactatt cctctgtcag gttataacat attgtgagct tagcttcaat ggtgtatgga
     3181 acaaatttca cctgtgaata aaggttagca gtagttattt tgagaatttt gcgccgtcag
     3241 tatttaccgg tacattatgt gataggaacc tttcttttaa ctcgttaaag catattttga
     3301 ttgatggccc aaaaacgtga ggggctaatt tattttgtca ctgtggtaac agatggctat
     3361 ggcattttcc gaaaatagca aagtatgaaa agattgattg gtgtttatgt taatattcgt
     3421 ttgaaggaat agcggactga tgatttggaa gcgtttaaat acaggtgctt tcgcaaggaa
     3481 aaattggaaa acgaccaggt aaaataggtt actaaataga gtggggtttt tcattgaaaa
     3541 aggagtgata acagacttgc tctgggttga tgatttggaa attgtataga tataaaaaac
     3601 gaatgtataa ataacagata atcaataatt aattaattga atgaaggtgt attgttgttt
     3661 tgaatcggcg taaatgattc tttgagattc ggattgataa aagttgtatg aatcacgcgg
     3721 tcaatcgttc tttcacattt ttaaagaaat ttgtttcttt gactaaaata aagtttaagt
     3781 agttttgtcc aaaaccctaa gaaatatccc tcttttttgg ttcagcatgt taggttactt
     3841 tgcctttgtg gagattattg tgttatggag ggatcctcag ttgttcctat ccgggcaaat
     3901 catgatgaac agactgattt acatcattat ctttggattg ctgcttatcc tgcagcggaa
     3961 attgcccgta aagcaatttc tctttttttc gatcctggca gcttatgccg cgcttacatt
     4021 gctttataag gaaactgccc tgctaaatcg cctcttctat cctgtgctgg atccctatct
     4081 gtctgaactt gattttcagt tgacgggaat tcaaccagcc ctggtatttt cagagcgatt
     4141 cgattcggtc tttttcagtg agttgatgtt tttgggctac ttctcctatt atttgactcc
     4201 gttggccatc ctgattttga ttttcttcaa aaaattcgag ttgatggaac agttcggttt
     4261 tgttctgatt acttccttct tattgtatta tctggtgttt atttttattc cggccatcgg
     4321 tccgcagttt tatttttctt atcctgataa tttcgttgaa gcaaagggtt tattcgggca
     4381 tctggtcaaa ctgattcaag agaacggtga ggcgccgaca gctgccttcc caagctcaca
     4441 tgtgggagta gcattgattt gtatgatctg gctgtggaga aatgcaaaaa aatatgtcta
     4501 ttatttcctt cctaatgttt ttttgttgat tttagctact gtttacatta aggcgcatta
     4561 tctggtggat gtactcgccg gtgcaatcac tgcacctttg gtattttttt tagttgttaa
     4621 gctatatgat caattgaaat atagatcgac cggtagcttg cctcaatgat tctgaatatg
     4681 attgttgatt ctttagcctt ttcttatgag tatcgaaata ttggaagtaa aaaataagag
     4741 tgatttaaaa tcctttattt acctaccgga aaaaatacac cgaaaccacg ctaattgggt
     4801 ccatccaatc tacctggacg aaaagcagtt tttcaatttt gacaaaaatg aagcttttcg
     4861 gcacaacagg acagccttgt ttttggccag ggaaaatgga agggtcgtgg gacgaattat
     4921 ggccatcatt ccggatgaat acaataatat gaaaaatgtc aggacagcaa ggttctgtta
     4981 ttttgaagct ccggaaaaca agcgaatatt tgatgctctt ttgaacaagg tggaacaatg
     5041 ggcgaaggat gccggttgta acctgatggt aggccctatg ggtttttcgg acaaggaacc
     5101 acagggcttc cttactgcgg gttataatga gaagacgatg attgtgacca actgtagctt
     5161 caggtacatg aaggagtttc tggaagccag ggattacagt cctttcgtgg aattgtgtca
     5221 gtatgatgtc cccctttcta atcaaatcat tcaaagatat cgtgcgctgg ccgacagggt
     5281 agaaagtaat atgcgggtga ctgtccatga atttaccaaa acatctgaaa taaagccatt
     5341 tgtgcaaagt gttttccggc ttatcaacac gacatatact gatatttatg gatttaccaa
     5401 ggtcactgaa aaagaagcag aagactttgc caaccgtttt attccccttt tgaatccaag
     5461 actaataaag ttgataactg acgaaagcgg ggaagtggtt gcattcgtta taggcatgcc
     5521 tgatctcagc ggagcaatca agaagagcag aggaagaatt ttccctttcg ggtggtttca
     5581 tattttaagg gcattcagga attctaaaag aatggttttg ctgttgggag ccgttaagaa
     5641 cgaaatgcag aacaaagggt tggatgcagt cctggcggtg cgcctcattc gttccgccct
     5701 ggatcttggt tttgatatta tggacagcca cctcatcatg aggaagaata tcaagatgag
     5761 acgggaaata gagcgcctgg agaatcaccg gatgtacaaa gaatacacca tatattcgaa
     5821 agaactctga tcggtatccc gggctgatta ttgacaaaag agactgaaga tgaatagaat
     5881 cagttctcat ttttaaaatg caccattata actccctgta cggtcaattt tttgattact
     5941 tttgccgttt aattttgcag acaaaacaac ctggcaaatt gggtaaaata gcgtttcgtt
     6001 tgcaaagctc cgcatggttt gtagtttggt cctggaacta tggctgaatc aggaaagcga
     6061 atggtaaaat cagtgttgga gcccgatgtg attgatttaa agcataatat caacaat
//
LOCUS       DAOQMC010000141         6096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5575_length_6096_cov_4.527727, whole genome shotgun sequence.
ACCESSION   DAOQMC010000141 DAOQMC010000000
VERSION     DAOQMC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5575_length_6096_cov_4.527727"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PKV31_06645"
     CDS             <1..874
                     /locus_tag="PKV31_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92570.1"
                     /translation="TTDDAAMLSFKIGGNGDMKAIRPPKMPQNDNIDFYPPEVLEDNE
                     EERSGEFFGNKTYVIDLTPKKAVDIEVKVPDFVYFDPKQKAYKSISLDPLQLSVSPGI
                     EKKSAGDEGPQIVSPQQSFFEKNKTGLLAATGIATLALAAFLLIRKKKNSEVTDKVPE
                     HRTDNLPPEPAGEAVIIPGKADEIPVKDFNRELDMANEFLFSGDQKSFIRQLYNTFSS
                     YLSDRLEIPLSQFNVNKIREVMEEKKLSKELVTKATDTLRVIESAAYGGLTPNFIPDQ
                     LLHSVKEIMEEISK"
     gene            complement(943..1413)
                     /locus_tag="PKV31_06650"
     CDS             complement(943..1413)
                     /locus_tag="PKV31_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92571.1"
                     /translation="MRFSFMMLAFLLVSCAHRKSMETSATLTPGIIFIGYKIFKGNDV
                     PAAQLRWYKLSEGTLKDNENKEPDIKAFHLELRDKEGKKVADRFLADVLLKNVEYVND
                     EGLFERQMIALDSADLIIRIPFERMPEKIVLSARGTKDSYITLHEFNLPIRSRN"
     gene            complement(1520..3037)
                     /locus_tag="PKV31_06655"
     CDS             complement(1520..3037)
                     /locus_tag="PKV31_06655"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNB92572.1"
                     /translation="MTHQYIRSLKDFEGKAIILKGWASNLRSGKGIYFILMRDGTGFC
                     QVVVSQDAVPAEAFDAASSLTQESSFWVEGTVVKDEKQVGGYELQATNLGIISISEEY
                     PISNKEHGVTFLSNNRHLWLRSRRQWAIMTIRNEIIMAIHGFFQKEGFVQMDPPIFTG
                     NAVEGTTTLFETDFFGHPAYLSQSGQLYAEAMAMAMGKVYTFGPTFRAEKSDTPRHLS
                     EFWMIEPEMAFYDLEDDMQLIEAFIKYVVAQVLEKCLPQLEILERDIDRLKNVFQTFP
                     RVTHADAAKILRGELEVNGKNALKVQEEDLAKAIEDLNKVQKEIEEREEKIKTASKGE
                     RRFHEGKILQLRKEVEILTEKTRNIPNWIESARNFSDDNDFGNSDETVLTRVFDAPVM
                     VYNWPAKIKSFYMKEVEGNPDLVKGVDLLAPDGFGEIIGGSEREDNLDILLRKVKEHD
                     LPMEVFQWYLDLRRFGSVPHAGFGLGLERVVRWICGLDHMRETIPFPRRYGRLFP"
     gene            3193..3909
                     /locus_tag="PKV31_06660"
     CDS             3193..3909
                     /locus_tag="PKV31_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92573.1"
                     /translation="MKKAIVVGATSGIGRKLAEILADNGYMVGITGRRGELLNEISTQ
                     KPHVFRTMVMDVNDTTTSISKLKALVDELGGLDLLVLSSGIGERNNRLDYEIEQAIVQ
                     TNVVGYTCIIDWAFNLFAAKKSGHLVGISSIAGLRGGQVGPAYGATKAYQINYLEALR
                     LRSARKKLGVTVTDIRPGFVDTAIIGGGKVFWMASTDKAGLQIYRAIKKKCKVAYITR
                     RWILVAWLLKLVPRFIYDRA"
     gene            4520..5320
                     /locus_tag="PKV31_06665"
     CDS             4520..5320
                     /locus_tag="PKV31_06665"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNB92574.1"
                     /translation="MERELTEVKNQSNYQNVAQHETSSLSDQAMFPAVPIQLSASTET
                     EPESTNGREEQNFKKPNNQSADASHYSGEATESFLNYSNSAIPAFSGAIQAKQQTTHG
                     TSFTAPVFQLKAYSEPISVQRSASGYFNSNPMPIQKKDDDENEIGKPNDGGQQSDSKN
                     SDNDVLSQMESAMNADFSNVKIHSSSHNAVNVGAQAFTQGNDIHFAPGKFDTNSPAGK
                     ELLAHELVHVKQQGEGRVQANNKIDGIPLNDDKKLEDEADEIASKVTR"
     gene            complement(5737..6063)
                     /locus_tag="PKV31_06670"
     CDS             complement(5737..6063)
                     /locus_tag="PKV31_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNB92575.1"
                     /translation="MLFVSDPAKEQINRVKANQGMSDDYFIRVSVVSGGCSGLSYKMD
                     FDNEEQAQDQVFEDNGIKIVTDLKSFLYLFNTTLQFSGGLSGKGFYFENPNAVRECNC
                     GESFAV"
BASE COUNT         1812 a         1376 c         1252 g         1656 t
ORIGIN      
        1 gaccacggat gatgcggcaa tgcttagttt caagattggc ggcaatggcg atatgaaggc
       61 tatccgaccg ccaaagatgc cgcagaatga taatatagac ttctatcccc ctgaagtgct
      121 ggaagataat gaagaggaac gatccggcga gtttttcggc aacaagacct atgtcattga
      181 tttgacaccg aagaaggctg ttgacatcga ggtcaaagtt ccggattttg tctatttcga
      241 tcccaaacag aaagcctaca agtccatctc cctggatcca ttgcagttga gtgtttcacc
      301 aggcatagaa aaaaagagtg caggagatga agggcctcag atagtaagcc cgcaacagtc
      361 cttctttgaa aaaaacaaaa cgggcctttt ggccgcgacg gggattgcca cacttgccct
      421 ggccgccttc ctgctgatta gaaagaaaaa gaatagcgaa gtaaccgaca aagtgcccga
      481 acatcgtacg gataacctgc cccctgaacc tgccggagaa gctgtcatca taccgggaaa
      541 agcggatgaa ataccggtga aggatttcaa ccgggaatta gatatggcca atgaattcct
      601 tttctcaggt gatcagaaat cattcatacg gcaactctac aatacattct cctcttacct
      661 aagtgatcgg ctcgaaattc cactgtcaca gttcaatgtc aataaaattc gggaagtaat
      721 ggaagagaaa aaattatcca aggagcttgt tacgaaggct actgatacac tccgggtaat
      781 tgaatctgct gcatatggag gattgacacc caattttata ccggatcaac tgttgcattc
      841 ggtaaaggaa ataatggaag aaatttcaaa gtaaggaaaa ccttccaggg atcatagctg
      901 gatgtattat ttcaaaaaac tggagatgaa tctctgaaaa aatcaattcc gggacctgat
      961 gggcagattg aattcatgta aagttatata gctatctttt gtcccccggg cagacaaaac
     1021 aatcttttcc ggcattcttt caaaaggtat tctgatgatc aggtcagcac tgtcaagggc
     1081 tatcatttgt cgttcaaaga gaccttcatc attgacatac tccacatttt tcagcaatac
     1141 atcagctaag aatcggtcgg ctactttctt tccttcctta tctcttaatt caaggtgaaa
     1201 tgccttgatg tcaggctctt tattttcatt atccttcaag gtgccttctg aaagtttata
     1261 ccaacgcaat tgggcggcag gtacatcgtt acccttgaat attttatatc caataaaaat
     1321 aatcccggga gttagggttg ctgatgtctc catcgattta cggtgagcac aggaaaccag
     1381 caagaaggcg agcatcataa agctgaatct cataggaaat aatttatttc agacaaagat
     1441 aactacggcc gctgacatcc ggcatgaaac ctgatcaccc catcttgtat agcatttttt
     1501 ggatgaagtt gcctaaaaat caaggaaata gccttccgta tcttcttggg aacggaatgg
     1561 tttcacgcat atgatccagt ccgcaaatcc aacgaacaac tctttccagt cctaagccga
     1621 accctgcatg aggtacactg ccaaagcgac gaagatcgag ataccattgg aatacttcca
     1681 tcggcaggtc atgctccttg actttgcgaa gcaggatatc cagattgtct tcacgctccg
     1741 agcctccgat gatttcccca aaaccatccg gagcaagaag atcgacgccc ttgaccaagt
     1801 ccggattgcc ctcaacttct ttcatataga atgatttgat ctttgccggc cagttatata
     1861 ccataaccgg agcatcgaaa acacgggtca ggacggtttc gtcactgttt ccaaagtcgt
     1921 tgtcatcgct aaaattacgc gctgactcaa tccaattagg tatattacgg gtcttctcag
     1981 tcagaatctc cacttccttt cttagctgga gaatcttccc ttcgtggaag cggcgttcgc
     2041 ccttggaggc agtctttatc ttctcttccc gctcttctat ttccttctgt accttgttga
     2101 ggtcttcaat agcttttgca aggtcttctt cctgtacctt gagtgcattc ttgccattga
     2161 cctcaagctc accacgcagt attttcgctg catcagcatg agttacacgt gggaaagtct
     2221 ggaatacatt cttcaatcga tcgatatctc tttccagaat ttcaagctgc ggcaagcact
     2281 tttccaacac ttgtgccacg acatacttga taaaagcttc gatcaactgc atatcatctt
     2341 ccagatcgta gaatgccatc tccggctcga tcatccagaa ttcagacagg tgacgaggcg
     2401 tgtctgattt ttcggctcgg aaggtcggcc cgaaggtata gaccttaccc atggccatgg
     2461 ccattgcttc ggcataaagc tggccggatt gggacaaata agccggatgg ccgaagaaat
     2521 cggtctcaaa cagggtcgtc gtcccctcta cggcattacc tgtaaagata ggcggatcca
     2581 tctgtacaaa accttctttc tgaaaaaatc catggatagc catgataatt tcgttcctta
     2641 tggtcatgat ggcccattgt ctgcgtgagc gcaaccaaag gtgacgattg ttggacaaaa
     2701 aggttactcc gtgttcctta ttggagatgg gatattcctc ggagatactg attataccaa
     2761 gattggtggc ttgtagttca taaccgccta cctgcttttc gtccttgacc actgttcctt
     2821 ccacccagaa tgaactttcc tgagtcaagc tgcttgcggc atcaaaagct tctgcaggta
     2881 cggcatcctg gctgactacc acctgacaaa acccggtacc atcgcgcatt aggataaaat
     2941 aaattccctt gcctgagcga agatttgacg cccagccctt cagaatgatg gctttccctt
     3001 cgaaatcttt aagcgatctg atatattgat gagtcattgc tatgatattt gaaggcgcaa
     3061 aagtacgatt ttttcaggaa ttaggaatga attcaacaaa gtaaacatca tcaatctcat
     3121 actcctacca acaggaacgt ctccaattcg aataattcat atatttacac atatctttat
     3181 aacttaaata caatgaaaaa ggcaattgta gtcggcgcca cctcaggtat agggcgcaaa
     3241 ctggcggaaa tacttgctga taatggatat atggtaggca tcaccggtcg tcgtggagag
     3301 ttactgaatg aaatcagcac tcaaaagccg catgtattca gaaccatggt aatggatgtc
     3361 aacgacacta ctacaagtat ttcaaaactg aaagccttag tagatgaact aggaggtttg
     3421 gatcttctcg ttcttagttc cggcatcggt gagcgtaaca atcgcctcga ttacgagata
     3481 gaacaagcga tagttcagac caatgtcgtt ggctatacct gcatcatcga ttgggcgttc
     3541 aatttgtttg cagcgaagaa atccgggcat ttggtgggca tcagttccat cgcagggctc
     3601 cggggtggac aggtaggtcc tgcttatggc gccactaaag cttatcagat caattactta
     3661 gaggcattga ggctaagatc agccaggaaa aagctgggtg tcaccgtcac cgacatacgg
     3721 cccggctttg tcgatacggc catcatcgga ggtggtaagg tattctggat ggcatccacg
     3781 gacaaggccg gtcttcagat ataccgggcg attaagaaga aatgcaaggt tgcctacatc
     3841 acacgacgtt ggatacttgt agcctggtta ctgaagctag tacccagatt tatttatgat
     3901 cgggcataat cttgtgggcc ggtgcaccgg cataatgttg tgtcgagaaa agcaaccaac
     3961 aaacccatta agttgtcctg cagtgatgga tagacaccca cctccggtaa gcaattcgga
     4021 ggttcccagg ttagtgccac tttagcctga tccatatcag gcgggagagt atccctctca
     4081 ttttgttagt gcgttcaaga tctaggtcat tatgatttac aggtatacac cttatctaac
     4141 tacaaacacc atattcatct tgcatatcct taaaattgga gcgaaaaaga gaaatctgtt
     4201 tcagtagaaa ttgctcttat acttagttac tttttattca tccacaattt gacaactaat
     4261 gcgatactat cccttgccaa atacatactt acaaatatgt ttggttagaa tacaattttt
     4321 ctgtccggaa ttttctcccg caaataaatc aaatagaaaa aaatatctga tcgatactct
     4381 tacttgctaa atcattcgga taaatgagtt ttggccatgt aataaatggc tatcaaattt
     4441 tattacaaaa taataattta tttaatcgca atttacgata atttttatta attttgtggt
     4501 tatgtctatc aattaaatta tggaaaggga gctaacagaa gtcaagaatc aatccaacta
     4561 ccaaaatgtt gcgcagcatg aaacgtcttc attaagtgat caggctatgt ttcctgctgt
     4621 acctattcaa ctttctgcct ctactgagac tgaaccggaa agtacgaatg gccgggaaga
     4681 acaaaacttc aaaaaaccga acaatcaatc agcagacgcc tcccattatt ccggtgaagc
     4741 tacagagagt ttcctcaatt attcaaattc ggcaattccc gcattttcgg gagcgattca
     4801 ggcaaaacaa caaaccacac atggcacttc cttcactgct ccggtttttc aattaaaggc
     4861 atattcagaa cctatttccg tgcagcgctc cgcttccggc tattttaatt caaaccccat
     4921 gcctattcag aagaaagatg atgatgaaaa tgagattgga aaaccaaatg atggggggca
     4981 gcaatcagac tctaaaaact ctgataatga tgttctctcg caaatggaga gtgccatgaa
     5041 tgctgatttt tcaaatgtca aaattcattc tagttcacat aacgcagtca atgttggagc
     5101 ccaggcattt acacagggaa atgatattca ctttgcaccc ggcaaattcg ataccaacag
     5161 cccggcagga aaagagttgt tggctcatga actcgtccat gtaaaacaac aaggagaagg
     5221 tcgtgttcag gccaataata aaattgacgg gatacctctg aatgacgata agaaactgga
     5281 agatgaagca gacgagatag cctcaaaagt gaccagatga agtttgataa ggaattacag
     5341 ggtattacct aagcagggga ttctaatgtt taatgtttca tcaaattcat agtggcatga
     5401 aactacggcc attctctcgg ccaatccttc cattaagcag cttctccggc agatctcctc
     5461 tgatatcatt cgtttgtttt tatcagatga tcttataact ataacacaag aacgtccggg
     5521 aaaaccaact gcctctttca ggcaccctgt cattggacaa gccaagatac ttcctaatgg
     5581 ctgattcgga ccatgtatca ttctggggac aatccgaaaa ccggtcagta attacgatca
     5641 attcaataaa tcaatagtcc atccgcacca ttactaatgg agtacaaaaa aagccggaaa
     5701 tatctcccgg ctaataaaaa tgtatatttt atcaaatcaa actgcaaagc tttcgccaca
     5761 gttgcattct ctgaccgcat tgggattttc aaagtaaaag cctttgccac tcaaaccacc
     5821 gctaaattgc aacgtagtgt tgaaaaggta cagaaagctt ttaaggtctg taacaatttt
     5881 gatgccattg tcttcaaaaa cctgatcctg agcctgttcc tcattatcaa aatccatctt
     5941 ataggacagc ccggaacatc cgccactcac tacagagaca cggataaaat aatcgtcgga
     6001 cattccctga ttggccttaa cccggttgat ttgctcctta gccgggtctg aaacgaataa
     6061 catagtttct gatttaaaaa gatattagaa attaag
//
LOCUS       DAOQMC010000142         6060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5627_length_6060_cov_4.682598, whole genome shotgun sequence.
ACCESSION   DAOQMC010000142 DAOQMC010000000
VERSION     DAOQMC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5627_length_6060_cov_4.682598"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKV31_06675"
     CDS             <1..452
                     /locus_tag="PKV31_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92576.1"
                     /translation="YRYKELPSGDYNYFSDTAHKIILKNLKECTNYEFGVFTLCQTDS
                     SDYKVINFKTDCTNASNQLDHSIHWKVFPNPFGNKINVLLTSNTYNEGVLRLVDTKGH
                     TVYHEKMSLSIGDHYINLDVDGNLPPGMYILTLTDGRGVKSAKLIKQ"
     gene            676..2148
                     /locus_tag="PKV31_06680"
     CDS             676..2148
                     /locus_tag="PKV31_06680"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNB92577.1"
                     /translation="MENNLSFKERNEHTLTEWRNKEMQALDLLQKAGELRFDRSVELV
                     LFRKDIYDTRPSELLNLHQNAANYGETEVTIEDTVAITNELVAMEDLRQARLDIGSLA
                     LEWKNNKEKYSSLGEFIGARLKTAQGAQSENIMPRDVILYGFGRIGRLVARRLVSQTG
                     RGEQLRLKAIVIRPQMKDRKQDAEKRAALLRTDSVHGEFAGSVRVSDDGSELIINGNR
                     VLLIYASYPSEIDYSALGINNALVIDNTGVFRTKEDLEQHLRPGVDHVILTAPGKKIP
                     NIVYGVNHESIDFNNDKVLCGASCTTNAIVPPIKVLDDAFGIEKGHVETIHSYTSDQN
                     LLDNFHKKPRRGRAAAINMVITSTGAAEAITAVLPYLDGKITGNAVRVPTPNVSLAII
                     NVSFGRPTTVDEVNNLLKQASLSGPLAEQIGFSNDPEYVSTNGVGMTTACVIDGPSTI
                     VSNDGKTVTVYAWYDNEFGYTCQVVRVAKHVGQVRRKTYY"
     gene            2150..2872
                     /locus_tag="PKV31_06685"
     CDS             2150..2872
                     /locus_tag="PKV31_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB92578.1"
                     /translation="MSDHREVQPIKKERSPFPQKSDFFTAISVDCVIFGLDRESLKVL
                     LIKSDFPLYEGKWSLLGDLIAKNEDINQAADRVLYERTGLQNLYLEQVMSFGEVKRHP
                     SGRVVSIAYYTLVNIDQINLLKKDHELHWHKIDQVGEMAFDHNKILNICLSRLQERIK
                     SEPIGFNLLPQKFSLRELQLLYESVLMVELDRRNFRKKMLSNGLLIDLKELEDDVPHR
                     PAKLFSFNRQKYDLTEIKGKFI"
     gene            2999..4156
                     /locus_tag="PKV31_06690"
     CDS             2999..4156
                     /locus_tag="PKV31_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB92579.1"
                     /translation="MKQFLSLLFAGLIGGAAAVGGIKYYESQSAPNIPEITETATAHQ
                     VNYAPVNVPFDFTQAAGKSLDAVVHIKAAESRQSAYQRQLQQQRTDPFSFFFGQRGPQ
                     QGSGSGVIISEDGYIVTNNHVVDFADQLEVTLHDGRVYEAVKVGTYPNADIAVIKIKA
                     NDLKPIKYGNSDDVKVGEWALAVGNPFDLTSTVTAGIISAKGRSIDIIHTKDAVENFI
                     QTDAAVNPGNSGGALVDINGNLIGINTAIATPTGTYAGYAFAIPVNQIIPIVENIIKN
                     KGDYNGPNLGAKATLGVTVANVTDLTPDQKEENGVIAKTGVLLLELQNGGSAQYSGLL
                     PNDVITGINNKAINNINDLKSTLSKLKPDDVISVSFKRGDQTKQLDVKLRS"
     gene            4353..4883
                     /locus_tag="PKV31_06695"
     CDS             4353..4883
                     /locus_tag="PKV31_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92580.1"
                     /translation="MSTEPKEYLQKMYSETLQASRFNTFRIFNTLIIALFVLLFMNVL
                     FTTPDNTFSYITLIIAVVSVLFMFYRLTRAKAGVKALPQDVSDIDGEIIRQHIDAIRI
                     SQRKLRWQMAVFTIPFFIYFLIDLVVSSTEIVEKKYLIMKIAFFFVLLILYYYLFFKI
                     RDTNFEERNSTKTQAD"
     gene            4940..5977
                     /locus_tag="PKV31_06700"
     CDS             4940..5977
                     /locus_tag="PKV31_06700"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92581.1"
                     /translation="MQKTVFFLIFCCIGLTSTAFSQNQYKVINIGFYNLENLFDTLDT
                     PKKMDEEFTPTGSRLYNTAVYLDKLTRLADVISLLGKEISPDGVAILGLAEIENRKVL
                     EDLTAHPKLKSSDYKIVHYESEDFRGIDVGLLYQPKYLTVLSSKPINVPILNDKGEKV
                     YTRQTLYVAGIMDGDTVHFLVNHWPSRRGGTSSVPLRMQAATIARTVKDSILTISPNA
                     KVIVMGDLNDDPTDKSITVGLKAFGKREKIKPDQMYNPYVDFYRKGIGTTPFQDAWSL
                     FDQVIITPGWLDNSNHGYIYHKARVFNEKFLTESSGKYKGYPKRTFSGDTYNFGYSDH
                     FPVYLTFVKKI"
BASE COUNT         1943 a         1206 c         1276 g         1635 t
ORIGIN      
        1 tataccggta taaggaactt ccttcaggag attacaacta cttctcagat acagcacaca
       61 agattatttt aaagaacctt aaagaatgta ccaattatga gtttggtgtc tttacactct
      121 gccaaacgga ttcaagtgat tataaagtaa tcaacttcaa gaccgattgt accaatgcca
      181 gcaatcaact ggatcattca attcactgga aggtattccc caatccattt ggaaataaaa
      241 tcaatgttct cctgacttcc aatacatata atgaaggcgt gttgcgcctt gtcgatacga
      301 aaggacatac ggtgtaccat gaaaaaatgt ccctgtccat tggagaccat tatatcaatc
      361 tcgatgttga cggaaattta ccgcccggga tgtacatcct gaccctgact gatggcaggg
      421 gggtaaaatc agcgaaattg attaaacaat aaaacaaaag gttgttccac ggagcaacct
      481 tttttaatta gcgactcaca gagcaggcat cagggaagcg atcccgcaat aggaataatc
      541 aaccagcaag gttaattccg ataatttaag caggaaaaat acgctttaag cttgaaataa
      601 tttattggat gccttaagaa acgggaaatt ttatatcttg tggcaaattc aaaaaacata
      661 tattgcaatc gagtcatgga aaacaattta agttttaaag aaagaaatga acatacgctg
      721 acagaatggc gtaacaagga aatgcaggca ttggacctgt tgcaaaaagc aggtgaacta
      781 agatttgaca gatctgttga actggttttg ttcagaaagg atatttatga tacccgcccc
      841 agtgaattgt tgaacttaca ccagaatgca gcaaattatg gagaaaccga agtgacgatt
      901 gaggatacgg tggccataac aaatgaattg gtggctatgg aggatctcag acaggctcgt
      961 ctggatatag gttctcttgc actggaatgg aaaaacaaca aggaaaaata ttctagtctc
     1021 ggtgaattca tcggggccag attaaaaaca gcacagggcg cccaatctga gaatattatg
     1081 cctcgtgatg taatcctgta tgggtttgga agaatcggaa ggttggtagc aaggaggtta
     1141 gtatctcaga cagggcgtgg agagcaattg cgtctcaaag ctatagtgat cagacctcaa
     1201 atgaaggaca ggaagcagga tgccgaaaaa cgggctgctc ttttacgaac agactccgtg
     1261 catggcgaat ttgcaggttc agttcgggta agcgatgatg gcagcgaact tataataaat
     1321 ggcaacagag tgttgttgat atatgcaagt tatccatcgg agattgatta ttcagcatta
     1381 ggaatcaata atgccctggt cattgacaat actggcgtat tcagaacgaa ggaagatctg
     1441 gaacagcatc ttcgtcccgg agtagatcat gtcatcctga ccgcaccggg taagaaaatt
     1501 cccaacatag tgtatggtgt aaatcatgaa tccattgatt ttaacaatga caaggtactt
     1561 tgcggggcct catgcaccac caatgccatt gttccaccta tcaaagtatt ggatgatgcg
     1621 tttggtattg agaagggcca tgttgagacc atccattcct atacaagtga ccaaaacctg
     1681 ctggacaact tccacaagaa gcctcgcagg ggacgtgctg cagcaatcaa tatggtgatc
     1741 accagtaccg gtgcagctga agccataacg gcagtacttc catatctgga cggaaagatt
     1801 accggcaatg ctgtcagggt acccacaccc aacgtatcac ttgccatcat caacgtaagt
     1861 tttggcagac caactaccgt tgatgaagtc aataatctac taaagcaggc atcgctgagc
     1921 gggccgttag cagagcaaat tggattttcc aatgacccgg agtatgtatc aaccaatggt
     1981 gttggcatga ccaccgcatg tgtaatagac ggtccttcca ccatagtgtc aaatgatgga
     2041 aagactgtca ctgtatatgc ctggtatgac aatgaattcg gctatacttg tcaggttgta
     2101 agggtagcta agcatgtcgg acaggtgaga agaaaaactt attattgatt tgtcagatca
     2161 tagagaagtc cagccaatca aaaaagagcg atcacctttt ccacagaaat cggatttttt
     2221 cactgccatt tctgtggact gtgtaatatt tggtcttgat cgggaatctc ttaaagtact
     2281 gcttatcaaa agtgatttcc cattgtatga agggaaatgg tcattgctcg gagacctgat
     2341 agcaaagaat gaagatatca atcaggcagc ggatcgtgta ctttatgaac gtaccgggct
     2401 gcaaaatctt tatcttgaac aagtcatgag ttttggagag gtaaaacgac atccctccgg
     2461 tcgggttgtg tccatagcct attatacact ggtcaatatc gatcagatca acctattgaa
     2521 aaaagatcat gaactacatt ggcacaagat agaccaagtc ggtgagatgg cattcgacca
     2581 taacaaaata ctgaatatat gcctgagccg attacaggag cggatcaaat ctgagccaat
     2641 aggattcaat ctcctgcccc agaaattttc tcttcgtgaa ttgcagttat tgtacgaatc
     2701 ggtattaatg gtggaactgg ataggaggaa tttccgcaaa aagatgctgt caaacgggct
     2761 attgattgac cttaaggaat tggaggatga tgtacctcac cgaccggcca agttattttc
     2821 ctttaatcgc cagaaatatg acctaacaga gataaaagga aaattcatct gaaagattgc
     2881 aggttaaaat attgttaata gttgccttgg aaggtaacct caaatccttt gtcggcgtta
     2941 aatggctaaa taatctaatt aaaattattc aaatcttata aaagcaatta ttgaatttat
     3001 gaaacaattc ctttcattat tgtttgccgg tttgatcgga ggtgctgcag cagtgggcgg
     3061 gataaaatat tatgagtctc aaagtgcacc aaatatacct gaaataacag agaccgccac
     3121 agcgcatcag gtaaattatg caccggttaa tgtacccttt gactttacac aggctgccgg
     3181 caaatcactg gatgccgtag tgcacatcaa ggcagcagaa agcagacaga gtgcgtatca
     3241 acgtcagcta caacagcaac gaactgatcc cttttcattt ttctttggtc aaagaggccc
     3301 ccagcaaggt tcgggctccg gagttatcat ttctgaagat ggctatattg tgaccaacaa
     3361 ccatgtggtt gattttgctg atcaattgga agttactttg catgatggta gagtatatga
     3421 agctgtaaaa gtaggtacct acccaaatgc cgacatagcc gtcatcaaga taaaggccaa
     3481 tgatctcaaa cccattaagt acggaaattc agacgatgtt aaagtaggtg aatgggcgtt
     3541 ggcagtaggt aacccattcg atctgacctc tacagtgaca gctggaatca tttctgctaa
     3601 gggaaggtcg atagacatca tccacactaa agatgccgtg gaaaacttta ttcagaccga
     3661 tgcggccgtc aatcccggca acagtggcgg ggccctggtg gatatcaatg gcaacctgat
     3721 cggaatcaat acagccatag caactccaac agggacatac gcaggatatg ctttcgctat
     3781 acctgtcaac caaataatcc ctatcgttga gaatattatt aaaaacaaag gagattataa
     3841 tgggcctaat ctgggcgcca aagcaacatt gggtgtcact gtcgccaatg tgactgactt
     3901 aactcccgac caaaaagagg agaatggtgt catagctaaa acgggagtac tcttgctcga
     3961 attgcaaaat ggtggttctg cacagtattc cggcttactc cccaatgatg tgatcaccgg
     4021 tattaacaac aaggccatca acaatataaa tgacctgaaa agcacattat ctaaattaaa
     4081 accggacgat gtcatatcag tttcgttcaa gagaggggac cagacaaaac aattggatgt
     4141 taaattacgt tcttaataaa agtaaagtca gcattaaatt gattgtaaaa taagttttcg
     4201 ttttgttttt gatctgagct gcctggagca tttgcctcag gcagtttttt ttatcaataa
     4261 aacatggaaa caggttaata tggaagaccc agatatttgt ttttcaatgt gattgaaatt
     4321 cactaatttt gtcatgcaaa atacgcatca ttatgtccac cgagcccaaa gagtatcttc
     4381 aaaagatgta ttccgagacg cttcaagcca gcagatttaa taccttcagg atattcaata
     4441 cgcttatcat agccttgttc gtattacttt ttatgaacgt cctgttcacc acacccgata
     4501 ataccttcag ctacattacc ctcatcatag ctgtggtatc tgtactcttc atgttttaca
     4561 gattaacgag agcaaaagcc ggagtcaaag cattacctca ggatgtaagc gatatcgatg
     4621 gcgaaatcat ccgccaacac attgatgcaa tccgaatcag ccaaagaaaa ttgagatggc
     4681 aaatggcagt ctttaccata ccctttttta tatattttct gattgacctt gtagtttcat
     4741 ccactgaaat cgtagagaag aaatatctga ttatgaagat tgcctttttc tttgttttgc
     4801 tgattcttta ttactatttg ttttttaaga tccgcgacac taattttgag gagcgcaatt
     4861 caaccaaaac acaagcagat taatttcttc catccactta aatcacactt aaatattgtc
     4921 aataaccaaa aacggaattt tgcaaaaaac tgtctttttt ctgatctttt gctgcatagg
     4981 gcttacttcc actgcattca gccaaaatca atacaaagta atcaatattg gattttataa
     5041 tctggagaat ctcttcgata cattggatac gccaaaaaaa atggatgaag agtttactcc
     5101 gacaggaagc aggttataca atacagccgt ctatctcgac aaactaacac ggcttgcgga
     5161 tgtgatatct ctgttgggca aggaaataag tcctgatggt gtcgctatac taggcctggc
     5221 tgaaattgaa aacagaaagg tacttgaaga cctcaccgca catcctaagc taaagtcatc
     5281 ggactacaag atagttcatt atgaatcaga ggatttcagg ggtattgatg ttggtcttct
     5341 ataccaacca aaatacctca ctgtgctaag cagtaagcct ataaatgtac caatattgaa
     5401 cgacaaagga gaaaaagtat ataccaggca gaccttgtat gttgcaggaa tcatggatgg
     5461 cgatacagtc cattttctgg tcaatcactg gccttccagg cgaggaggca cttcatccgt
     5521 gccactgaga atgcaagctg ccacaatagc gcgtactgtc aaagattcta ttctgactat
     5581 aagcccaaat gccaaggtta tagtcatggg tgacctcaat gatgatccaa cggacaaatc
     5641 aattactgtc gggctgaaag cctttggaaa acgtgaaaaa attaagcctg accagatgta
     5701 taatccatat gttgattttt atagaaaggg aattggcaca actccatttc aggatgcctg
     5761 gagtctgttt gaccaggtca tcattactcc cggatggctt gataattcca accatggata
     5821 tatctatcac aaagctcgtg tattcaacga aaaattcttg actgaaagtt caggcaaata
     5881 caagggttat cctaagcgta ctttttccgg agatacatac aattttggat atagtgacca
     5941 cttcccggtt tatttaactt ttgtaaaaaa aatataagac cattacattt gtggttcaat
     6001 aataccaagt ggtcataaca atcatttcag ggagtccttc aaaaaacagc gtttcaatac
//
LOCUS       DAOQMC010000143         6002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5701_length_6002_cov_4.960821, whole genome shotgun sequence.
ACCESSION   DAOQMC010000143 DAOQMC010000000
VERSION     DAOQMC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5701_length_6002_cov_4.960821"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKV31_06705"
     CDS             complement(<1..294)
                     /locus_tag="PKV31_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92582.1"
                     /translation="MQRPDHRLSFKPFFIRGMVVVFLSFASWCNYALYGQQRITHSVD
                     FSSYQSKSVEGLNTHGILLFPQSEITIFNKVALVSTSLPIAKGKRAKIIDFKPY"
     gene            703..1704
                     /locus_tag="PKV31_06710"
     CDS             703..1704
                     /locus_tag="PKV31_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNB92583.1"
                     /translation="MRLNILILGFILFSFVANSQDPVFSQFYAHPLELNPALAGNSGG
                     TRFGINYRNQFSGLPSNYTTYAISADQYFPGIRSGVGISVMADEAGQGIYRTYNGELS
                     YTYGVEFRNGTKVKMGLQAGFISVTLDYDKFIFMDQIDPIHGPISPGGIPYPTAEAPP
                     DFRNRTLFDMGAGGLLSNDKFYVGIGLKHLNRPDINFYAAENNTKKGLSMRISAMAGF
                     RIPIGDIRYGHRSEMAIIPNLLIVKQANLGQINAGALLEIKALQFGLYYRNGFLNPDA
                     IILSVGYNSGQFKVAYSYDATISKLGINSGGAHELSLSYVLFQDDGGKYHDCTKIFR"
     gene            1922..3421
                     /locus_tag="PKV31_06715"
     CDS             1922..3421
                     /locus_tag="PKV31_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNB92584.1"
                     /translation="MNQAFKIFILFGCVLFLFASCKKKEERSSTTGWVYNDQKWGGFT
                     KSNAIDQPTGPNLVLVEGGTFTMGMTQEDVTYEWNSVPRRVTVSSFYMDETEVANIDY
                     REYVFWTKRVFGDNYPQVWRNTLPDTMCWREELAFNEPYVETYFRFPSYDDYPVVGVS
                     WIQANEYCKWRSDRVNEMMLIEKGILNPNPEQKDSDNFNTQAYLYGQYEGSVKKNLKD
                     VSTGGERKVTYEDGILLPAYRLPTEAEWEYAALALQGNMLSAKDEIVTDRRVLPWNGD
                     SPRYQKRNKYMGKLMANFKLAGGDYMGVSGNLNDNSSIPSTVRSYFPNDFGLYNMAGN
                     VSEWVADVYRPTNSITLRDVDNQDLNPFRGNEFKQMVVGPDGIPVEKDSLGRLRYELM
                     ADSTIADRENFKKADVRSYLDGDNAIDGAYEYGVTSLISDKSRVIKGGSWADRLFWLS
                     PGTRRFKDEDKSDRTIGFRCAMTRMGSSEGNGQNSGNIFGKQKTVKRKY"
     gene            3487..4758
                     /gene="murF"
                     /locus_tag="PKV31_06720"
     CDS             3487..4758
                     /gene="murF"
                     /locus_tag="PKV31_06720"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNB92585.1"
                     /translation="MASLLKYFVEHPSVTIDSRNIKPGQIFFAIKGEHFDGNDFVMQA
                     LQEGAAYCIASNPVFHGHDRIIVVDDTLEALQDLAREYRGTFKFPVLAITGSNGKTTS
                     KELLSAVLSTKFKVHCTYGNLNNHLGVPLTLLATPPDTDFAVVEMGANHQGEIRELCA
                     IAKPDYGCITNIGIAHLEGFGGLDGVRKGKSELFEYLMEHHGTFFINVQENSLDFLND
                     PNNEQLVRISTETEEITILKPTTDFLSFGWYDLNVDTHLVGQYNFNNILIALGVGRYF
                     GCDGRKMCEAISSYVPRNNRSQMIEYRGAQVIMDAYNANPSSVISALDNLIQNPGFNK
                     GLVLGDMLELGEDSAEYHRKILEYIRKADVFSPVMLIGPRFRQFEQAFPEFQFFGDTD
                     EAVSMIDWKSMAGYTVLVKGSRGVRLEKLVN"
     gene            complement(4836..5573)
                     /locus_tag="PKV31_06725"
     CDS             complement(4836..5573)
                     /locus_tag="PKV31_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92586.1"
                     /translation="MEALDIACISPNVQLFHNSSTVCHEVFSEINPRCLENIVNIGIS
                     ATQTHLSSKSDLTPQYFDSYRLGQMIHQMEVINDAIAFADIVVADISAIKQSDIPGRR
                     NISSSGLSSEAFNHIARNAGTGKSRVTVLTGLESLDPGDDIGIDTLAQFVFYFLDGAH
                     SQSSGTDEEILSSYTIDECLPYESVNFLKSEFTGRWFAEFPTRLPDHLKKFQLIPCSY
                     QDYQYSARGELSPRILDIFKRLDELVN"
BASE COUNT         1858 a         1096 c         1340 g         1708 t
ORIGIN      
        1 atatggcttg aagtcgataa ttttggctcg cttccctttt gcaattggta acgaagtgga
       61 tactaatgcc accttgttga aaatggttat ttcactctga ggaaatagca ggattccatg
      121 tgtattcaat ccctcaactg acttggactg ataggatgag aaatcaacgg aatgtgtaat
      181 cctctgttga ccataaagtg cataattgca ccaagaggca aaggataaga acacaaccac
      241 cattcctcgt ataaaaaaag gcttgaaaga aaggcgatga tccggacgct gcatatgaag
      301 gtcgttgtat ttcaacaaaa ttaataaaat ggtagtagga tatatttaaa ataaaatcag
      361 aatacttaaa acgagtatgg aatcaaatta ttatcaaatc atcaacaaag ctattaattt
      421 aacacatgct cattatccgt aatgaattaa aatttgatgt tatccaaaaa ctataccaaa
      481 ttttgttttt aaattaaaaa aataaataat atattacaag ttgcccggaa atcctggtta
      541 gtttgggagg aatactttta taagcaagta agtaaactga ttaattcgcc tttgggaatt
      601 aatatttttt caaatgcaca agctgtaatc catctctgcc tctaaaaaga aaatcaagta
      661 aatataaaaa acgttacatt tgacatttca aacgggtaag taatgagatt gaatattctg
      721 atactgggat tcatactgtt ttcctttgtg gctaatagcc aggatccggt tttttcccaa
      781 ttttatgccc accccctcga gttgaatcct gcactggcag gtaacagcgg aggtaccaga
      841 tttgggatca attaccggaa tcaattctcc ggacttcctt ccaactacac cacctatgcc
      901 ataagtgcag atcaatattt tccgggtatt cgcagtggtg tgggtatttc agtcatggcg
      961 gatgaggcag gccagggaat ttacagaaca tataatggcg agctgtccta tacgtatggc
     1021 gtggagttca gaaacggaac taaagtaaag atgggacttc aggccggatt tataagcgtc
     1081 acacttgatt atgacaagtt tattttcatg gatcaaatag atccaattca cgggccgata
     1141 agtcccggtg gtatccctta cccgacagca gaagcacctc ctgacttccg caacaggacg
     1201 cttttcgata tgggagccgg aggtttactg agcaatgaca aattctatgt aggtattggc
     1261 ctgaaacacc ttaatagacc ggatatcaat ttttatgctg cagagaacaa taccaaaaaa
     1321 ggactatcta tgaggatttc tgccatggcc ggattcagga ttccgatagg tgacataaga
     1381 tatggccatc gatcggaaat ggcaattata ccgaaccttc tcatcgtcaa gcaagcaaat
     1441 cttggacaga taaatgccgg tgccctgttg gaaatcaaag cattacaatt cggattatat
     1501 tatagaaacg gctttctgaa tccggatgcc atcatcctta gtgtcggtta taattcagga
     1561 caattcaaag tggcatatag ctatgatgcc accatcagca aactgggcat caattccgga
     1621 ggagcccatg agttgagcct cagttacgtc ttgtttcaag atgacggagg taaatatcac
     1681 gactgcacga agatttttag ataaatatgc atttttacaa aacataatat cataaaaagg
     1741 taaaaaatac gtttaaaaaa agtgtaggta aaattttgta cagaattaat tagttgtatc
     1801 tttgaccttc caaaattcaa acaagtagat ttaacagagt ttttggattc atattgagtt
     1861 tacttacgct aaatcaataa gttgcaaaat ttctggaatt ttttaaaact gaaaaattat
     1921 aatgaatcag gcattcaaaa tttttattct ttttggctgt gttctcttcc tttttgcatc
     1981 atgtaagaag aaagaagagc gctcaagcac gaccggctgg gtatataatg accagaaatg
     2041 gggtggcttt accaaatcca atgccatcga tcagccaaca gggccaaacc tggttttggt
     2101 tgaaggtggt acttttacga tgggtatgac ccaggaagac gtgacctacg aatggaacag
     2161 cgtgccccgc agggtgaccg tgtcttcatt ttatatggat gagaccgaag tggccaacat
     2221 tgattacaga gaatatgttt tctggaccaa gagagttttt ggcgacaatt atccacaggt
     2281 ttggagaaat acacttccgg acaccatgtg ctggcgagaa gaattggcat tcaacgagcc
     2341 ttatgtggaa acttatttca ggtttccctc ctatgatgat tatccggtag tgggcgtttc
     2401 ctggattcag gctaatgaat actgtaaatg gagatctgac agagtcaatg aaatgatgct
     2461 catcgagaaa ggcattctca atcccaatcc tgagcagaag gacagcgaca actttaatac
     2521 acaggcttat ctttacgggc agtatgaagg cagtgtaaag aaaaacctta aagatgtttc
     2581 gaccggaggt gagcgtaagg ttacctatga agacggtatc ctgcttcctg cttaccgact
     2641 tccgacagaa gcggaatggg agtacgcagc actggcatta cagggtaata tgctttccgc
     2701 caaggacgaa atcgttactg atagacgagt attgccttgg aacggtgact caccacgtta
     2761 ccagaagaga aataagtaca tgggtaagct gatggccaac tttaagctgg caggtggtga
     2821 ttacatggga gtttcaggca atctgaacga taactcgtcc attccatcta ctgtaaggtc
     2881 atacttcccc aatgacttcg gcctttacaa tatggctggt aatgtttccg aatgggtagc
     2941 agatgtatac agaccaacca actcaattac actaagagat gtggacaatc aggatttgaa
     3001 cccattcagg ggtaatgagt ttaagcaaat ggttgttggt cctgacggaa ttccggtaga
     3061 gaaagacagt ctggggcgtt tgagatatga gcttatggct gattccacaa tcgctgacag
     3121 agaaaacttc aaaaaagcgg atgtgcgtag ctatctggat ggtgataatg ctatcgatgg
     3181 tgcttatgaa tatggtgtaa cctctttgat atccgataaa tcaagagtta tcaagggtgg
     3241 atcttgggcg gatcgtttat tctggctttc tccgggaaca agaaggttta aagatgaaga
     3301 caagtcagat aggaccattg gtttccgatg cgctatgacc agaatgggta gctcagaagg
     3361 taatggtcaa aatagtggca acatctttgg aaagcaaaag acagtcaaga gaaaatatta
     3421 atttttgatt aacgttatac gaatcctcac aattaacttt gtggggattt tttttatctt
     3481 aacataatgg catcattgct gaaatatttt gttgaacatc catcggtcac aattgacagc
     3541 aggaacatca aaccgggtca gattttcttt gcgataaagg gcgagcattt cgatggcaat
     3601 gattttgtca tgcaagcctt gcaagaggga gcggcatatt gtatagcatc taatccggtt
     3661 tttcatggcc acgaccgaat catagttgtt gatgatacgc ttgaggcact tcaggatttg
     3721 gcacgagaat atagaggaac tttcaaattt ccggtactgg ccatcaccgg ttccaatggc
     3781 aagacgacat caaaggaact attgtcagcc gtattgagca ccaaattcaa agtgcattgt
     3841 acgtacggca atctgaacaa ccatctcggt gtgccattga ccctgcttgc tactcctccg
     3901 gacacagact ttgcagtcgt agagatgggg gccaatcacc agggggagat cagggaatta
     3961 tgcgctattg caaaacctga ttatggttgt attaccaata ttgggatagc acatctcgaa
     4021 ggattcggag ggttggatgg cgtcaggaaa ggcaaatccg agcttttcga atatctgatg
     4081 gagcatcacg gcactttctt tatcaatgtg caagagaata gcctggattt tttaaatgac
     4141 ccaaacaatg aacaacttgt gaggatcagt actgagacgg aagagattac gatacttaag
     4201 ccgacaacgg actttttgag tttcggttgg tatgatttaa acgtggatac tcaccttgtc
     4261 ggccaatata acttcaataa tatcctgata gccttagggg taggtcgata ttttggctgt
     4321 gatggcagaa agatgtgtga agctatttca tcctatgttc ccagaaacaa tcgttcgcaa
     4381 atgatagagt atcgaggggc gcaggttatc atggatgcct ataatgccaa tccgtcatcg
     4441 gtaatatccg cccttgacaa tctgattcaa aacccgggct ttaataaagg acttgtattg
     4501 ggcgatatgc ttgagctcgg agaagattcg gcagaatatc accgtaaaat actcgaatat
     4561 attcgtaagg cagatgtttt ttctccggtc atgctgatag gtccgcgatt caggcaattt
     4621 gaacaagcat ttccggagtt tcaatttttc ggagacacag atgaagctgt atccatgatt
     4681 gactggaaat caatggcggg gtatacggta ctggtaaaag gatccagggg ggtacggttg
     4741 gaaaaattgg taaactgaga aagcaatgct tctctcatgg caaatccttg ctatcctaaa
     4801 tcatttcatc ggcaatgaaa tcctgctgac aacaatcagt tgaccaactc atccagtcgt
     4861 ttgaaaatgt ccagaattct tggtgagagc tctccgcgtg ctgaatactg ataatcctga
     4921 taactgcaag gaatcagttg aaactttttc aaatggtcag gaagacgtgt ggggaattcg
     4981 gcaaaccaac gtcctgtgaa ttcactcttt agaaagttga cactttcata tggcaggcat
     5041 tcgtcgattg tgtatgagga gagtatttct tcatccgtac cagaagattg ggaatgggca
     5101 ccatcgagaa aataaaaaac aaactgggcg agggtatcga taccaatgtc atctcccggg
     5161 tcaagacttt ccagtccggt caaaacagtg acacgcgatt tgccggtgcc ggcatttcgg
     5221 gctatatgat tgaatgcttc tgaactaaga ccgcttgagg agatattcct tctgccgggt
     5281 atatcgcttt gttttattgc tgaaatgtcc gcaactacta tgtcggcaaa tgcaatggca
     5341 tcatttatga cttccatttg atggatcatc tggcctaatc gataggaatc aaagtattgc
     5401 ggtgtaagat ctgacttact gcttaagtga gtttgagtag cagaaatgcc tatatttacg
     5461 atattttcca ggcacctggg atttatttcg gaaaagacct catggcagac ggtcgaagag
     5521 ttgtgaaaaa gttggacatt tgggcttatg caggcgatat cgagagcttc caataaaaat
     5581 tttaattcgg atagtttgtt ttgtaaaata aaaattttac atttgctatt gtttaaaatt
     5641 tcactaaaca aagaaaattg ccggtccgtt tcctttaata ttcccgggaa aaaacaatgg
     5701 gaatattttt gattgttgcg gtaggattta agcataacca gaattttttc agcaactgaa
     5761 ttgtcggttg atagaagtat aatgttctga ggggaggaaa agaattcgga agtagggcga
     5821 gtatcacaca ccttgaaatc gattgaattt aaaaagctat agttatcaat catattggat
     5881 agttgattta taatttacgc gttaaagatg aatatttttt cgcaaatagt aaaaaaatta
     5941 tacctttgtg gtaatatata taattgattt ttaatgttaa agtaaattcc tatgaccaag
     6001 aa
//
LOCUS       DAOQMC010000144         5999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5708_length_5999_cov_4.132234, whole genome shotgun sequence.
ACCESSION   DAOQMC010000144 DAOQMC010000000
VERSION     DAOQMC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_5999_cov_4.132234"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2064
                     /locus_tag="PKV31_06730"
     CDS             <1..2064
                     /locus_tag="PKV31_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNB92587.1"
                     /translation="TWLLFGFILLAIAVWFRNTNPQRWTLKGIFPEFNHNFFSFLNFS
                     SWIESWKRKVGDDEYEQQIEEELSAINSGADEVITGVEVDNDGFERIESLNQVEPGKV
                     EPSHVPFELDEEVTPEILNDASIQEVDDYGFEIEEPHSSFGETIQSSEISPIVEPPIN
                     ELSPDDEELLKINAELDNPDEPYDPVLELSRYIKPGLDLLDSYEDQQVQIDHAVLNAN
                     KDQIVNTLLNYKIEIVKIRATIGPTVTLYEIVPAPGVRISKIKNLEDDIALSLAALGI
                     RIIAPIPGRGTIGIEVPNQNKQIVGLRDVLNSERFQTAQMDLPIAFGKTISNEVFVAD
                     LAKMPHLLIAGATGQGKSVGINAILLSIIQHNHPAYVKLVLIDPKKVELSIYSRLENH
                     FLCMLPDQDEPIITDTKKVIRTLNSLVIEMDSRYDLLKKAGVRNIREYNQKFTERKLN
                     PNKGHRFLPFIVLVIDEFADLIMTAGKEIETPIARIAQLARAIGIHLVIVTQRPSVKI
                     ITGIIKANFPARIAFKVSSKIDSGIILDAGGADQLIGRGDMLLSADGKIVRIQSAFVD
                     THEVDRVVQFISDQQGFATPYMLPDVPDDSGDGGEKGQLKLSDLDDLIQEAALLVVTN
                     QHGSTSMIQRRLKLGYNRAGRIMDQLEALGIVGENEGSKPREVLFHSEAEVNHFFANL
                     NQTTF"
     gene            2575..2859
                     /locus_tag="PKV31_06735"
     CDS             2575..2859
                     /locus_tag="PKV31_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HNB92588.1"
                     /translation="MAERILTLGKHPLSTYKDYIQHASIKEINTIGMSDRMMVKEILQ
                     DMTTLIELEREIMDAASSAGDEGTNDMVNAFMQFKEKSCWMLRAFIGNEK"
     gene            3060..5336
                     /locus_tag="PKV31_06740"
     CDS             3060..5336
                     /locus_tag="PKV31_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB92589.1"
                     /translation="MRDPNWIGVSPSSLRWGADGTLYFKYNPESAEADSLYSLNTNDM
                     PYFSKVSDQSQEVLYEDNLIRNPSDPSMVVYNRQGNIFLRRMKGDVKKIFSTHEYEWA
                     TGFSSDGKYLVYKIGNDMFSYNIEKGFIRQLLAIQSASPRDNKKKLSNQDQVLMEEQK
                     NLFDVFIQEKKVSKKSKEKRDYFVYERGSADVVGTSISNDLLIAAIRLKDGRTESHTT
                     IMPKYVTESGYTETVNTRAKVGIIQPDYSLKFYNFEKDTLMDFLVNDLPGINAMPNYL
                     EEYPVLHDSLAKASAKKSVNIFDPLWSPNGHLAVVQVYSFDNKDRWIVLFDADSGIMK
                     IIDHQHDEAWIGGPNIGGNYWPGWCVWLDDDHVAFTSEISGYAHLYKYEISSGKIIAL
                     TSGNYEVQRVTLAPDRKSLYLLTNQNHPGTKTFVKLALNSLKQIVLLNPDCGLDEVTV
                     SPDGKWIAYLQSTTNQPWELFISENKAGGKSTRITDKAISREFGEYPWQSPKVITFRA
                     SDGREVYARLYKSRVANPNKPGVIFVHGAGYLQNAHKWWSSYFREYMFHNLLTDLGYT
                     VLDIDYRASSGYGRDWRTAIYRHMGGTDLSDQVDGVKYLKDSAGVNINNIGIYGGSYG
                     GFITLMALFTKPGVFQAGAALRPVTDWMHYNHGYTSNILNVPALDSIAYARSSPINFV
                     DGLKDRLLICHGMVDDNVHFQDVVRLQQVLIDKGKDNWELAAYPMEAHGFKTPSAWTD
                     EYKRILKLFEETINRKSN"
     gene            complement(5352..>5999)
                     /gene="rsmI"
                     /locus_tag="PKV31_06745"
     CDS             complement(5352..>5999)
                     /gene="rsmI"
                     /locus_tag="PKV31_06745"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNB92590.1"
                     /translation="AIETLKHVTYIYAEDTRTTGVLLKKYAIDTPLRAFHSFNEHKQI
                     EPILEVVKSGNDIALVSDAGTPGISDPGYLLVHHLIESGVEVTCLPGPTALIPALVMS
                     GLPADKFYFEGFLPHKKGRQTRLQAICDMPCTVILYESPFRLMKCIEELIEIAGEERP
                     CCIVREISKIHEQAITASLGTLLHKLKSGKIPAKGEIVLVLGGKPNEKKSRSLNK"
BASE COUNT         1768 a         1138 c         1335 g         1758 t
ORIGIN      
        1 acctggttgt tgttcggatt tatcctactt gccattgctg tatggttcag aaatacaaat
       61 ccgcaacgct ggacgctgaa aggcattttc ccagaattca accacaattt tttctccttc
      121 cttaatttct cttcctggat cgaatcatgg aaaaggaagg tcggagatga cgaatatgaa
      181 caacaaattg aggaagaatt gtcagctatc aatagcggag ctgatgaggt tatcacgggg
      241 gtggaggttg ataatgacgg ctttgagcga attgaatcat taaatcaagt cgaaccgggt
      301 aaagttgaac catcgcatgt tcccttcgaa ttggacgaag aagtcacacc cgaaatatta
      361 aatgacgctt ccattcaaga ggtggatgac tatggctttg aaatagaaga acctcattct
      421 tcgtttgggg aaacaataca atcttcagaa atctctccga tagttgagcc tccaatcaat
      481 gaattgtccc cggacgacga agaattgctc aagatcaatg ccgaactgga taatccggat
      541 gagccgtatg accctgtgct ggaattatcc cgttatatca aaccgggatt agatctgctg
      601 gatagctatg aagatcagca agtacagata gaccatgcag tgttaaatgc gaataaggat
      661 cagatcgtca atacattgct caattataag attgaaatcg tcaagattag agcgactatt
      721 gggccaactg tcaccttgta cgaaatagtt cctgccccgg gagtcaggat ttcaaagatt
      781 aagaatctgg aagatgatat agctttatct cttgcagcac ttggtatccg tatcattgca
      841 cctataccgg gaagaggtac catcggtatt gaagtgccga atcaaaataa gcaaatcgtt
      901 ggtctaaggg atgtacttaa ttctgagcgt tttcaaacag ctcagatgga cttaccgatt
      961 gcctttggaa agacgatcag caatgaagtt tttgtggcag atctggctaa gatgccacac
     1021 cttctgattg caggggcaac cggacaaggt aagtctgtgg gtatcaacgc aatccttttg
     1081 tccattatac agcataacca tcctgcttat gtcaaacttg tcttgatcga ccccaagaaa
     1141 gtggaattgt ccatttacag cagattggaa aatcatttcc tttgcatgct tccggatcag
     1201 gatgaaccaa tcattaccga tacaaagaaa gttatcagga ccctcaactc ccttgttata
     1261 gagatggatt cgcgttatga tttgttgaaa aaagccggtg tacgaaatat cagggagtac
     1321 aatcaaaagt ttacggagag gaaactcaac cccaataaag gtcatcgttt cttgccattc
     1381 attgttcttg tgatcgacga gtttgcagac ctcattatga cggcaggaaa agaaatcgag
     1441 acccccatag cgcgtatcgc tcagttggct cgtgctattg gcatccatct tgtcattgtt
     1501 acgcagaggc catcggtcaa gatcattacc ggtattataa aagcaaactt ccccgcacgt
     1561 atcgcattta aagtatcttc caagatcgat tccggtatta ttctggatgc aggtggggct
     1621 gaccagttga ttggtagagg tgatatgtta ctttcagcag atggtaagat agtcagaatt
     1681 caatcggcct tcgttgatac gcatgaagtt gacagagtcg tgcaattcat ttcagatcag
     1741 cagggatttg caacaccgta tatgctgcct gatgtacctg atgattccgg ggacggtgga
     1801 gagaaaggtc agctgaaact atctgatttg gatgatttga tccaggaggc cgcactgctt
     1861 gttgtcacca atcaacatgg ttctacttcc atgatccagc gaaggcttaa gttgggttac
     1921 aatcgagcag ggcgtatcat ggaccaattg gaagctttgg gcattgtcgg tgaaaatgaa
     1981 ggcagtaagc caagggaggt acttttccat agtgaggcgg aggtaaatca tttcttcgcc
     2041 aatttaaatc agacgacctt ttaagcgatt ccatttattt cagtcaatcc atttggttga
     2101 agggttataa gcacaaacaa cgtattttta tatgcgttaa agtttaatgt ctattgattc
     2161 ttcaggtcga atttttctcg catttccttc ttttggacat ttccttataa taacaaatat
     2221 caggtcgctg atgaattgca gtaattcaag ttgtttctta aacaattctt gtattatgtt
     2281 gttgtacaat tagttagagg caggtcgtgg tacctttcat ttgtcatgac gcacctgtaa
     2341 cattctaata taactaattt aaaaatatca atctattatg gaagtaaatg agatcagttt
     2401 gagtaagaat caggttaagc ctgttatcga cttgttgaat gattatttgg caaattatca
     2461 aatccattat caaaagcttc gcggggctca ctggaatatt gaaggagaga attttttaca
     2521 ctgtatttga aatttgagga gttctagacc aatgcccaga caactatcga tgaaattgct
     2581 gaaagaatcc tgaccctggg caagcatcct ttgagtacat ataaagatta cattcaacat
     2641 gccagtatca aggaaatcaa tacaattgga atgagtgaca ggatgatggt taaggaaata
     2701 ctccaggata tgaccaccct gatcgaattg gaacgggaaa tcatggatgc tgcatcctca
     2761 gcgggagacg agggcaccaa tgacatggtt aatgccttca tgcaattcaa ggaaaaaagt
     2821 tgttggatgc taagagcttt tattggaaat gagaaataac atctgaccgg aaaaggatgg
     2881 gaatccatcc ttttttttat gaaatacttt tttgtcgata tttcacctat taagaatcta
     2941 ttatacctta tcttgcctta caaagctttt tcatcatgat ccggtgtact attatttact
     3001 ttttttgcct tcttgtcaac caatgttttt ctcagggtcc tctttctgtg gaatacatca
     3061 tgagagatcc taactggatt ggagtctcac catcatcgct gagatggggc gcagatggca
     3121 cgctttactt caaatacaat ccggagagtg ctgaagcgga ctctctatac tccttgaata
     3181 ccaacgatat gccatacttt tccaaagtgt cggatcaaag tcaggaggtt ctttatgagg
     3241 ataatttgat caggaacccc tcggatcctt ccatggtcgt atacaaccgt cagggaaata
     3301 ttttcttacg gagaatgaaa ggtgatgtaa aaaaaatctt ctcaacacat gaatatgagt
     3361 gggcgacagg ttttagctcg gacggaaaat atttagtgta taaaatcgga aatgatatgt
     3421 tttcttataa tatagaaaaa ggctttatcc gacagttatt ggctattcaa tcagcttccc
     3481 cgcgagataa taaaaagaaa ctatccaatc aggatcaagt gttgatggaa gaacaaaaga
     3541 atctattcga tgtctttatt caggaaaaga aagtctctaa aaagagtaaa gaaaaacggg
     3601 attattttgt atatgaaaga ggcagtgcag atgttgtggg tacatccatc agtaatgatc
     3661 tgcttatcgc tgccatacgc ttaaaagatg gccggacaga aagtcatact accattatgc
     3721 caaaatacgt tacggaaagt ggctatactg aaacggttaa tacaagggct aaggttggta
     3781 ttatccagcc tgattacagc ctgaagttct ataactttga gaaggatact ttaatggatt
     3841 ttttggtaaa tgatttaccc ggaattaatg ctatgccgaa ttaccttgag gaatatccgg
     3901 tcctgcatga tagccttgct aaagcatctg ctaaaaaatc agtaaatatt tttgatcccc
     3961 tttggtcacc caatggccat ttagctgttg ttcaggtgta ttcctttgat aacaaagaca
     4021 ggtggattgt cctatttgat gccgattcgg gtatcatgaa gatcattgat catcagcatg
     4081 atgaggcctg gatcggaggg ccgaatatag gtggaaatta ttggccgggc tggtgtgttt
     4141 ggcttgatga tgaccatgta gcattcacat ctgaaatttc cggatatgct cacctttata
     4201 agtatgaaat ttcttcaggt aagattattg cacttacatc cggcaactat gaggttcaac
     4261 gggtgacttt ggccccggat cggaagagtc tgtacttact tacaaatcaa aaccatcctg
     4321 gtacgaagac atttgtaaaa ctggcattga attctctgaa acagattgtt ttgttgaatc
     4381 cggattgcgg attggatgaa gtgacagtat ctccggatgg aaaatggatt gcctatctgc
     4441 aaagtaccac taaccagccc tgggaattat tcatcagcga aaataaagcc ggaggcaaat
     4501 cgactcggat cactgacaaa gctataagta gggagtttgg tgagtatcca tggcagtcac
     4561 cgaaagtcat tacattcagg gcatcagatg gcagggaagt ctatgccagg ttgtacaaat
     4621 ccagggttgc aaatcctaac aaaccaggtg tcatcttcgt gcatggtgcc ggatatcttc
     4681 agaatgcgca taaatggtgg agctcttatt ttagagaata tatgttccac aaccttctta
     4741 cagatcttgg ttatacggtt ctggacattg attatagggc gagctcaggg tacggcaggg
     4801 attggagaac tgcgatatac agacatatgg gagggactga tctttctgat caggtagatg
     4861 gcgtcaaata tttaaaggat agtgctggag tcaatataaa taatatcggc atttatggtg
     4921 gttcgtatgg tggttttatc accttgatgg ctctatttac aaagcccgga gtttttcagg
     4981 ccggagcagc tcttcgtcct gtgaccgact ggatgcatta caatcatggc tatacttcta
     5041 atattttaaa tgttccggcg ttggattcca ttgcttatgc aagaagcagc cctatcaact
     5101 tcgtggatgg gctgaaagac agactactga tttgccatgg tatggtcgac gataatgtac
     5161 attttcagga tgttgtcagg ttacagcaag tgctgattga taaaggcaag gataattggg
     5221 aattagcagc atatccgatg gaagcgcatg ggttcaaaac accttcagcc tggacagatg
     5281 aatacaaacg tattcttaaa ttatttgaag aaaccatcaa tcgcaaaagc aattaagcca
     5341 ttgtaaagat attacttatt tagactgcgg gacttctttt cattgggctt gccccccaat
     5401 acaaggacga tttcaccttt tgcaggtatt ttgcctgatt ttaatttatg gaggagcgta
     5461 cccaatgatg cagtaatggc ttgttcatgt attttggaaa tttcccgaac gatacaacag
     5521 ggtctttctt ctccggcgat ttctatcaat tcctctatgc atttcattaa ccgaaacggc
     5581 gattcataaa ggataactgt acagggcata tcacatatcg cctgcaatct ggtctgtcgt
     5641 ccctttttat gtggcaggaa accttcaaaa taaaatttat ctgcaggaag tccactcatg
     5701 acaagtgcag gtattaatgc tgtaggcccg ggtaagcagg tgacttccac tccggactct
     5761 ataagatgat gaaccaacag atatccgggg tctgatattc cgggagttcc ggcatcagaa
     5821 accagagcta tatcatttcc acttttcact acttcgagaa ttggttctat ttgtttgtgt
     5881 tcgttgaagg aatgaaaggc acgcaaaggt gtatcaatcg cgtatttttt taatagaaca
     5941 cctgtagttc gggtgtcctc agcatatatg taagtcacat gtttcagagt ctcaatggc
//
LOCUS       DAOQMC010000145         5967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5765_length_5967_cov_4.902064, whole genome shotgun sequence.
ACCESSION   DAOQMC010000145 DAOQMC010000000
VERSION     DAOQMC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_5967_cov_4.902064"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKV31_06750"
     CDS             <1..798
                     /locus_tag="PKV31_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HNB92591.1"
                     /translation="HKDDCKKWGLTPGTQGKRIVLQGLGNVGSHTGKICQQEGDMKII
                     AISEYEGAIYNENGIDITELLKHRKETGSILNFPGTKILPSRESALELDCDILIPAAM
                     ENQITSDNADKIKAKIVAEAANGPVSADGEAILLKKGIIIIPDMYLNAGGVTVSYFEW
                     LKNLSHMRFGRMEKRFDQNTYSNLVTAIENTTGKKINAQEREFLTKGAEEVDLVRSGL
                     EETMINSYNQIRETWKKKKSIEDLRTAAFICALNKISSDYLAMGIFP"
     gene            853..1341
                     /locus_tag="PKV31_06755"
     CDS             853..1341
                     /locus_tag="PKV31_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNB92592.1"
                     /translation="MIQNHSDNKLDKIDLKIIKILQENSKITNLDLSKKIGLSPAPTL
                     ERVKKLESTGVIASYHAAIDTLSLGLNVKTFVLVSLAWQRENALDKFLIKIKGISEIT
                     ECYIITGEADFLMKIVCKDIPSYEHILFKTLSQIEEIERLKTLMTLSTVKDSKILPFD
                     YE"
     gene            1344..2126
                     /locus_tag="PKV31_06760"
     CDS             1344..2126
                     /locus_tag="PKV31_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNB92593.1"
                     /translation="MAIIYKVTSPSNSFKSYLMGTFHVFVPEFSPLIEKAEKLISKCS
                     HYYGEMDLTQVDPLIINETMQSLPRPGKEVLYLLENQQPYFEKMGLPFPAYPAQNSIF
                     NLYSELTMSMWRKKILVGSLDEELIKIAMKNHIPIGGIESFNDQLEIMKSIDYEKMEK
                     QIIKMLIKPKSFSKNMDHLLDFYLKGKMNVLSKKSKGSSRGNRKLMVKDRNRVFADTI
                     ARICEESDRNFISFGAGHLGGKDGVIRRLKKAGFKVRKVSLD"
     gene            complement(2141..3700)
                     /locus_tag="PKV31_06765"
     CDS             complement(2141..3700)
                     /locus_tag="PKV31_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNB92594.1"
                     /translation="MEKIKILWVDDEIETLKPQLMFIQQKGYEVTAVTNGYDAMEEFE
                     SDNEFDAVFLDESMPGISGLETLSRIKAIKPNIPVVMITKNEEEHIMEEAIGAQIADY
                     LIKPVNPNQILLSLKRIVDEKRLIREKTSSDYQQDFRQIFMKINSGLDHQEWVDVYKK
                     IINWEIKMDQSQSLEMGDILSSQKAEANTAFCKFVDRDYMEWLDKDNKEAPVMSYNLL
                     REKVFPWVNDEMPTVVVLMDNLRYDQWKVIEPIINECYKVESEDYFFSILPTSTQYSR
                     NAIFAGMMPSEIEKKYPDLWKNDVDEGGKNLSENVFLADQITRVFRKPIKHEYQKITN
                     IQNGKALADNALNFLNNNLSIIVYNFIDMLSHARTEMEVLKELADDEKAYRSLTKSWF
                     IHSPLWLALQRLAEKPIRLFILTDHGSIKVKSPSKVVGDRETTTNLRYKVGRNLNYVG
                     NEVIDFKDPSKVYLPRPNVSSSFIFAKEDKFFLYPNNYNYYNNYYKDTFQHGGASLEE
                     IICPVIRLKSR"
     gene            4065..5270
                     /locus_tag="PKV31_06770"
     CDS             4065..5270
                     /locus_tag="PKV31_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB92595.1"
                     /translation="MRFPIRKIINDPVYGMVKIPDGIIFRVFEHPYFQRLRRIGQAGL
                     TNLVYPGANHTRFHHALGAMHLAIEALDMLELRNVDISEDEATGLILAILLHDVGHGP
                     FSHVLEHNLTGQHHEVLTLAIMNKMNMELEGALDTAVQIFTDKHPKKFLHQLISGQLD
                     LDRMDYLNRDSFFTGVAEGKIGYDRIIKMITVVDNNLVIQEKGIYSVENFLMARRLMY
                     WQVYLHKTVLAAEHMLIQCLRQARESVEKGEKFNISPHFLYFMQQKSFINEDEMLGHF
                     LKLDDYDLWWAIKQFAESRDQSLAFLADAILNRKIFKIELSKTPYSDEKITELVQQVG
                     NTFHLDEKHAAALVFHGSETNEMYNSKKDEIMVLMKDNSVRPFSEVSDYPVDKTEVVK
                     FFLCYPKLS"
     gene            5350..>5967
                     /locus_tag="PKV31_06775"
     CDS             5350..>5967
                     /locus_tag="PKV31_06775"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNB92596.1"
                     /translation="MAFTLPELSYEYDGLQPYIDAETMRIHHTKHHNAYINNLNAAIE
                     GTEFANMTIEEILENAGRAGAAIRNNGGGHYNHTLFWKLLSPNGTKPSGEALDGINKK
                     WGSFDGFKEAFSKAAATRFGSGWAWLVVNKDGSFDINSTANQDNPMMGKEVSGVEGFP
                     ILGLDVWEHAYYLNYQNRRPDYIEAFFNVIDWDEVNSRLVQSTEYF"
BASE COUNT         1921 a         1240 c         1072 g         1734 t
ORIGIN      
        1 cacaaagatg attgtaaaaa atggggcctt actcccggca cccaaggaaa aagaattgtt
       61 cttcaaggtt taggtaatgt tggaagccac accggtaaaa tatgccaaca ggaaggcgat
      121 atgaaaatta ttgcgatatc tgaatacgaa ggcgccattt acaatgaaaa cggcatagat
      181 atcaccgaat tgctcaaaca cagaaaagaa accggatcca ttcttaactt ccctggaact
      241 aaaatcctcc catcacgcga atcagcacta gaacttgatt gtgacattct gattcctgct
      301 gctatggaaa accagataac ttccgacaac gcagacaaga taaaagccaa aattgtggca
      361 gaagccgcca atgggcctgt ctccgcagat ggtgaagcta tcctgctaaa aaaaggtatt
      421 atcatcatac ccgatatgta cctcaacgcc ggcggtgtaa ctgtttccta tttcgagtgg
      481 cttaaaaacc tgtctcatat gcgattcgga agaatggaaa agagattcga tcagaacaca
      541 tattccaatc tggtaactgc aattgaaaat acaaccggta aaaaaatcaa tgctcaggaa
      601 agagaattcc tgactaaagg agctgaggag gtggatcttg taagatcagg tttggaggaa
      661 acaatgataa attcttacaa tcaaatccgt gaaacatgga aaaagaaaaa aagcattgaa
      721 gatcttagaa ccgcagcatt catttgtgca ctaaataaaa tcagtagtga ctacctggct
      781 atgggaattt tcccataatt ataacatcta aacattcttc aataattatt tctcattttt
      841 aaacgaattt ttatgattca aaaccattca gataataaac tcgataaaat cgacctgaaa
      901 attataaaaa tacttcagga aaatagcaag attactaatc tggatttatc caaaaaaatt
      961 ggattgtcgc ctgccccaac acttgaaaga gtcaagaaac ttgagtcaac cggtgttata
     1021 gccagctacc atgctgcaat agacaccctg tctcttggtc ttaatgttaa gaccttcgtc
     1081 ttggtttctc ttgcatggca aagggaaaat gcattggata aattccttat caaaatcaaa
     1141 ggcatctcgg agataacaga atgctacatc ataaccggag aagctgattt tctcatgaag
     1201 attgtatgta aggatatccc ttcctatgag catatattat ttaaaactct tagccagatt
     1261 gaggaaatcg aacgccttaa gacactcatg accctgtcta cggtcaagga ttctaaaatt
     1321 cttcctttcg attacgaata atcatggcta tcatttataa agtcaccagc ccatcaaata
     1381 gctttaaaag ttatttgatg ggtacctttc atgtttttgt gcctgaattc agtcctttga
     1441 ttgaaaaagc cgaaaaactt atctccaagt gctctcatta ttatggagag atggatttga
     1501 ctcaagtgga cccgctaatc atcaatgaaa ccatgcaaag cttgcccaga ccaggcaagg
     1561 aggttcttta tttacttgag aatcaacaac cctattttga aaaaatggga ctacccttcc
     1621 cggcctatcc ggcacaaaac tccatattca acctatactc tgaactaact atgagtatgt
     1681 ggcgaaaaaa aattcttgtc ggaagccttg atgaggaact tattaaaatt gccatgaaaa
     1741 atcatatccc aattggggga atagaaagct tcaatgatca attggaaatc atgaaatcaa
     1801 ttgattatga aaaaatggaa aaacagatca tcaaaatgct gattaaaccc aaatcatttt
     1861 ccaaaaacat ggatcacctt cttgacttct atctgaaagg aaaaatgaat gttctgagta
     1921 aaaaatctaa aggctcctca agaggtaaca gaaaactgat ggtaaaggac cgtaacagag
     1981 tctttgctga tacaattgcc cgcatttgtg aagaatcaga ccgaaatttc atttccttcg
     2041 gcgccggtca cctcggcgga aaggatggag tcatccgcag actaaaaaaa gccggtttta
     2101 aagtcagaaa agtcagtctt gattaatcac ttattacctg ttaccttgat ttcaatctga
     2161 tcaccggaca tattatttcc tcaagagatg ctcctccatg ctgaaaagtg tccttataat
     2221 aattattata atagttgtaa ttattaggat acaggaaaaa cttgtcctct ttggcaaaaa
     2281 tgaatgacga acttacattt ggcctcggta gataaacttt ggaaggatct ttaaaatcta
     2341 ttacctcgtt cccaacataa ttaaggttcc ttccgacttt ataccttaga ttggtggttg
     2401 tctcccggtc accgaccacc ttagaaggag atttgacctt gatggagccg tggtctgtca
     2461 gaataaacaa tcgaattggc ttctcggcca aacgttgaag agcaagccaa agcggagaat
     2521 ggataaacca ggacttggtg agtgaacgat aagccttttc atcatctgcc agctctttca
     2581 atacctccat ctctgtccgg gcgtgagaca acatatcgat aaagttatat acaattatac
     2641 tcaggttatt gttgagaaag ttgagcgcgt tatcagccaa agcctttcca ttctgaatat
     2701 ttgtaatctt ctggtattca tgctttatag gcttacgaaa taccctggtg atttgatcag
     2761 ctaaaaaaac gttctccgaa agattcttgc ctccctcatc aacgtcgttt ttccataagt
     2821 ctggatactt tttctcgatc tcacttggca tcattcccgc aaaaatcgca ttcctgctat
     2881 attgtgttga agtaggcaaa atgctgaaaa aataatcttc agattctacc ttataacact
     2941 cattgattat aggctctata accttccact gatcatatct tagattatcc atcaacacca
     3001 ctacagtcgg catctcgtca ttcacccaag ggaagacttt ttccctgagc aaattatacg
     3061 acataacagg cgcttcttta ttatccttat ccaaccattc catgtaatcc cgatccacaa
     3121 atttgcaaaa tgccgtattg gcttcagctt tctgagaact cagtatatca cccatctcaa
     3181 gcgattgact ctgatccatt ttgatttccc aattgatgat cttcttatat acatccaccc
     3241 attcttgatg atcgagaccg ctattgatct tcataaatat ctggcgaaaa tcctgttgat
     3301 agtcactgga agtcttttct cttatcagac gcttttcatc tacgattctt ttcagagaca
     3361 atagaatttg attgggattg accggcttta tcagataatc ggcaatttgc gctcctatag
     3421 cctcctccat gatatgctct tcctcatttt tagtgatcat tacgaccgga atattgggct
     3481 tgattgcctt tattctcgac aaggtctcca agccggaaat acccggcata ctctcatcca
     3541 gaaatacagc atcaaactca ttgtcactct caaactcctc cattgcatca tatccattgg
     3601 tcacagccgt tacttcatac cctttctgtt gaataaacat taactgaggc tttaatgtct
     3661 ctatctcatc atcaacccac aaaatcttta ttttctccat ctatttatat tattcgttga
     3721 atgaaaaatt ttaaactata tttcataata caaataaacg acaagattcg ttcattattt
     3781 gatggataca ttattttttc aaagtctgga gatattctat aaggtatttt tactttctgc
     3841 cagatccgga aaaccaatat tcgattaaac tcattatttg aaaagagcag gacgaagagt
     3901 tataaactac tctttaccaa cataattccg gctttcaaat gcctcttatc aatctaaaag
     3961 gaaaaattta ccgattccta ttttaattat ccacattatt ttcccattca aagcaaaatt
     4021 taagacatca tacaattacc tttatggcac aaacaatcta atgcatgcgt tttccaataa
     4081 gaaaaattat taacgatccc gtatatggca tggttaaaat cccggatggc atcatttttc
     4141 gggtttttga acatccatat ttccagcgtc ttagacgaat tggacaggca ggcctgacaa
     4201 acctcgtata tccgggcgcc aatcatacca gattccatca tgcattaggt gccatgcacc
     4261 ttgccattga ggccctggac atgctggaac ttaggaatgt tgatatttca gaagatgaag
     4321 ctaccgggct catactcgcc attttactcc atgatgttgg acatggacca tttagtcatg
     4381 tattggaaca caacctcaca ggacagcatc atgaagtgct aacattagca ataatgaata
     4441 aaatgaacat ggagttggaa ggtgcattgg atacggcagt acagattttt acagataagc
     4501 atcctaagaa atttcttcac caattgattt ccggacaact agacctggac aggatggatt
     4561 acctcaaccg ggattccttc tttaccggag tagcagaagg aaaaatagga tatgacagaa
     4621 tcatcaaaat gataactgtt gtagacaata atctcgttat ccaagaaaaa ggcatctact
     4681 ccgttgaaaa tttcctgatg gcacgccggc ttatgtattg gcaggtatat ctgcacaaga
     4741 cagtccttgc agcagaacat atgctcatcc agtgcctacg ccaggctcgt gaaagcgtgg
     4801 agaagggcga aaaattcaat atttctcctc actttctata ttttatgcag caaaagtcct
     4861 tcataaatga agatgaaatg ctcggacatt tcctgaaact ggatgactat gacttgtggt
     4921 gggcgatcaa gcaatttgct gaaagtcggg accaatccct cgctttttta gcagatgcca
     4981 ttctcaacag aaaaattttc aagatagaat tgagtaaaac accctattca gatgaaaaaa
     5041 taaccgaatt agttcaacaa gtagggaata catttcatct ggatgaaaaa catgccgctg
     5101 ctctcgtctt ccatggttct gaaaccaatg aaatgtacaa tagcaaaaag gacgaaatca
     5161 tggttcttat gaaggataat tcagtcaggc cattttctga agtgtccgat tatccggttg
     5221 ataaaacaga agttgtaaag tttttcttat gctaccctaa actttcttga atacttttaa
     5281 gttgtttagt ttattacgtt gcaaatttta acccggtcaa atattttttt aactttaaat
     5341 aattatacaa tggcttttac acttcctgaa ttgagttatg aatatgatgg actccaaccg
     5401 tatatcgatg ctgagacgat gaggattcac cacaccaaac accacaatgc ctatatcaat
     5461 aatctcaatg cagcaataga aggtactgaa tttgccaaca tgactattga ggaaatcctt
     5521 gaaaatgcag gtagagccgg cgctgctatc agaaacaacg gcggcggtca ttacaatcac
     5581 accttgtttt ggaagttgct ttctccaaat ggtactaaac cttcaggaga agcattggat
     5641 ggtatcaaca aaaaatgggg atcatttgat ggtttcaagg aggctttctc taaagcggct
     5701 gctacccgat ttgggtccgg atgggcatgg cttgttgtaa ataaagatgg ttctttcgat
     5761 atcaactcca ctgccaacca ggacaatcca atgatgggca aagaagtaag cggagttgaa
     5821 ggctttccga tcctgggcct cgatgtttgg gaacacgcct attatctaaa ttaccagaac
     5881 agaagacccg actatattga ggcattcttc aatgttattg attgggatga agtcaatagc
     5941 cgcctagtcc aatcaaccga atatttc
//
LOCUS       DAOQMC010000146         5931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5835_length_5931_cov_5.119809, whole genome shotgun sequence.
ACCESSION   DAOQMC010000146 DAOQMC010000000
VERSION     DAOQMC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_5931_cov_5.119809"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKV31_06780"
     CDS             <1..303
                     /locus_tag="PKV31_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNB92597.1"
                     /translation="KFAPFKEYYLGEGNYIVGRYSIDSIANIAILTTDKLVSRQHCEI
                     TGRQNADGGIDFTIKDAGSKNFIYINNRQLESDEEIYLNEKDIIRLGRIELQFHRD"
     gene            313..1020
                     /locus_tag="PKV31_06785"
     CDS             313..1020
                     /locus_tag="PKV31_06785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HNB92598.1"
                     /translation="MQIETFQHLGRREEQQDAYFISSDNKLFLVCDGAGGMENGALAS
                     NLAIKTISEAYSRQSGILDLADFKSMIYESIYKINSLLDKNVATTLVCLYIDNEYALY
                     CHLGDSKLFYFNEKNNSWIATKDHSIVQELFDAGIIKTEEEILNHPLRNRITKAITPG
                     IMPAPDDLEVEKIKNLNPGSIFVLCSDGVLEKTTNSAFAGVFNQQTSDLNEKVEIIRH
                     QCVQKSADNCTLIAIEI"
     gene            1055..2776
                     /locus_tag="PKV31_06790"
     CDS             1055..2776
                     /locus_tag="PKV31_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNB92599.1"
                     /translation="MRFIILSLSLFLFTNFVSAQKQVILYYDGNGIRSSKAECAYYKV
                     INYNKAGKIIGKIKDFYKSGEGYTTSDGAKEINRDNDNKSVYWGKQIRYYKSGAMKAI
                     RLFSLDGDIIYEKKWYSSGKIKSFGSYKTKYGSTFIEFAEDGSIIASYRNRKGEGDPR
                     VFVQKDNQYLIAFMEDFSTNQNRQFWEYEMPLEEGQFEIGVGYHIITEQNGHFAKILP
                     FSDSFDDSYMFSTNIGSIKGDKNTFHGIVYDYSGPDQFSCFGINGMGKFCIYHVNKSK
                     SFFEVPITDNSNINTGRNNNNLTIQYLDEKISFLINDVEVYTSNKNEFGEKYFGLFSD
                     GGRKDVVFQNISLLYLNEEIRTMMDDMIVGDGAFKTGTGFIIDDRGYMITNHHVIENA
                     DFIEVEFKWEGKTKTFPARIVSTDKEKDLSILRIISNDFKLPKKIPFAIEKADIPTGS
                     EVFTLGYPEAFNMGREVKFTDGKISALSGIKNNKSVYQTSIPARGGNSGGAVFGPNGN
                     IIGVLYAVRSGSEVYSYAIKSSVLIEFIQSFNKDIVITRPNTIKNQPLVSKVNTLKDY
                     VALVKVW"
     gene            2843..4324
                     /locus_tag="PKV31_06795"
     CDS             2843..4324
                     /locus_tag="PKV31_06795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB92600.1"
                     /translation="MTQEEFFTRYSIDFDNGWLGEGSFGTVYKAYDNYLNIWKAVKIA
                     KVRIIDGKQFSLVSEFQAIKSLPLHPNIAYYENVYQFQMSNGLFDYAVMQYYKDGNLK
                     QFLQSRTLDYESKRQVVEGLLQGVIFLHENNIIHRDIKPGNVMIAIHPQGMIIPKITD
                     FGLSRIKDGDISFMTGSIGGGTLEYSSPEQLYGKPISFNTDLWSFGVIVFEVMTGMKP
                     FYDDMSGNTEAQRLKLYQNITTGNISEKINTIPTPYKEIIELCLEADVRKRVDKGNIL
                     LNYLRKYDKVGATTGDMDEETYVMTKAEREEYEHIRDSHDINDLRMFVGKFPNSIFYG
                     EISARISSIQGNKHNGNQHPQSGLHKSTDKREHSKDTLTPHQSNSMAHISLKDRINRL
                     LARYRRYSYRDFRTSNTKFLYLIPILFFSFIVGYWAYSHFSKVEIYSEKGKYGLQSQL
                     KGIIYEAVFDTIIPLENGHFRVIQFNKTFIVDGMGNSQVDDTK"
     gene            4529..5044
                     /locus_tag="PKV31_06800"
     CDS             4529..5044
                     /locus_tag="PKV31_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB92601.1"
                     /translation="MSHSDNTTSDLSLETNRLLIRPIQETDAAFMLILMNTRGWLDYI
                     GDRHITSLLLAKKYIAGLLREGKSHYFVVSEKDSGKEVGVLSLVQRDYLEYVDLGFAF
                     LPQFHGKGMAYEAASSLMARVKDINQTILAVTTPDNTASQALIEKLNFSFLKTIFRHD
                     QPLLLYIHTTN"
     gene            complement(5356..>5931)
                     /locus_tag="PKV31_06805"
     CDS             complement(5356..>5931)
                     /locus_tag="PKV31_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92602.1"
                     /translation="KDDKWELIKANVSGGASNYCNLNVGLGGKLIATFRDGKNDNKVS
                     AMMYDGIEWQYLGKPGFTNASSYISMAIDPEGNPWVAYQDFGANKRLSVKKYINLVSS
                     VKNPKNEEPAIWLFPNPTNGEFTLRYSAGSHYRIFDICGKTLIKGTLHQAGTNQTIKE
                     QTIDAGNLGSGIYFITVEQGGLSKTLKFIKL"
BASE COUNT         1859 a         1162 c         1194 g         1716 t
ORIGIN      
        1 aaattcgccc ctttcaagga atattatctc ggcgaaggta actatatcgt cggcagatac
       61 agtattgact ccattgcaaa tattgccatc ctcaccactg ataaattggt atcaaggcaa
      121 cattgtgaaa tcactgggag gcaaaatgcc gatgggggca ttgattttac aattaaggat
      181 gcgggaagta aaaacttcat ctatatcaac aaccggcaac tggaatctga tgaggaaatc
      241 tatctcaatg aaaaagatat catcagactg ggaagaattg aacttcaatt tcatcgggat
      301 taaaaacgag ctatgcagat tgaaactttt caacatttag gaagaagaga agaacagcaa
      361 gatgcctact ttatttcttc tgacaataag ctttttttgg tatgtgatgg tgccggtgga
      421 atggaaaacg gagcccttgc cagcaacctg gctataaaaa caatctctga ggcttatagc
      481 cgtcaatccg gtatcctgga tcttgctgat ttcaaatcca tgatatatga aagtatctac
      541 aaaataaaca gcctcctgga caaaaatgtc gcaacaaccc tggtatgctt gtatattgac
      601 aatgaatatg ccttatattg ccatctcggc gacagtaagt tgttctactt caatgaaaag
      661 aataattcat ggatagccac caaagatcat tctatcgttc aagaactttt tgatgcagga
      721 attataaaaa ccgaagaaga aatcctcaac cacccactca gaaacagaat tacaaaggcg
      781 attactcccg gaatcatgcc tgcccctgac gaccttgagg ttgaaaaaat caaaaatctg
      841 aacccgggaa gtatctttgt actatgttct gatggtgtgt tggaaaaaac tacgaatagc
      901 gcttttgccg gagtattcaa tcaacagacg tcagatttga atgaaaaggt tgaaattatc
      961 aggcatcaat gtgtccaaaa atcagccgat aattgtacct tgatcgcgat agagatttag
     1021 caaaaacatt atccattgac aataaccaaa ccttatgaga tttattattc tttccctttc
     1081 cttgtttctt ttcaccaatt tcgtttctgc tcaaaagcag gttattcttt attatgacgg
     1141 caatggaata cgatcctcca aagcagaatg tgcttattat aaagtgatca actataacaa
     1201 agccgggaaa attatcggta agattaagga cttttataag tcgggagagg gatataccac
     1261 ctccgatggg gccaaggaaa tcaaccggga caatgacaac aagtccgtat attggggtaa
     1321 gcaaatcagg tattacaagt ctggcgccat gaaggctatt cgcctgttta gccttgatgg
     1381 tgatattatt tatgaaaaga aatggtattc cagtggcaag atcaagtcat tcggatccta
     1441 taaaactaaa tacggatcta ctttcataga gtttgcagaa gacggatcca tcatagcaag
     1501 ttatagaaac cgcaagggag aaggcgatcc gagggttttt gtacaaaaag ataatcaata
     1561 tctaatcgcc tttatggaag atttctccac caaccaaaac cggcaattct gggaatatga
     1621 aatgcccttg gaagaaggtc aatttgaaat tggagtagga taccacataa taacagaaca
     1681 aaatgggcat tttgccaaga tcctgccatt ttcggattcc tttgacgatt catacatgtt
     1741 cagtacaaac ataggatcta taaaaggcga caaaaacact tttcacggca ttgtatatga
     1801 ttattccgga cccgatcaat tttcctgctt tggtatcaat ggtatgggca agttctgcat
     1861 ctatcatgtc aataagtcca aaagcttttt tgaagtcccg ataacagata acagcaacat
     1921 taataccggc aggaataaca acaaccttac aatccaatat ttggatgaaa agatttcatt
     1981 tttaataaat gacgttgaag tatatacttc caacaaaaat gaattcgggg aaaaatattt
     2041 cggcttgttt tcggatggtg gaagaaagga tgtcgtgttc cagaatatat cattactcta
     2101 cctgaacgaa gaaatacgga ccatgatgga cgacatgata gtaggagatg gagcatttaa
     2161 aacaggcacc ggctttatta tagatgacag aggatatatg attaccaatc atcacgtcat
     2221 tgaaaatgcc gattttatcg aggtagaatt taaatgggaa ggaaagacaa aaacctttcc
     2281 tgccaggatc gtgagtaccg acaaggaaaa agacctttct attttgcgta tcatttccaa
     2341 tgatttcaaa cttcctaaaa agatcccctt tgccatcgaa aaagcggaca tccctaccgg
     2401 ttctgaagtg tttacgcttg gttatcccga agcattcaat atgggtaggg aagtgaagtt
     2461 taccgatggc aagattagtg ccctgtccgg aattaaaaac aacaagtctg tataccaaac
     2521 ttcgattccg gcccggggcg gaaattccgg tggagcagta tttggtccca atggaaatat
     2581 tataggcgta ctgtatgctg ttcggagcgg ttcggaagtt tattcttatg ccataaaatc
     2641 atccgtcctg atcgaattca tacaatcatt taacaaggat atcgtcatca cacgacccaa
     2701 tacgatcaag aatcagccat tggtttcgaa ggtcaatacc ttgaaagatt atgtagcgtt
     2761 ggtcaaggtt tggtgatttg ttgaatttcg tcatttaaat ttatatcact atttttactt
     2821 caaaaagtaa aaataagcac tcttgactca ggaagaattc ttcacgcgat attcgattga
     2881 ctttgacaac ggatggttgg gagaggggtc tttcggtacc gtgtacaagg catatgataa
     2941 ttatctcaac atatggaagg cagtcaagat tgcaaaggtg cgcatcattg acggcaagca
     3001 gttttcattg gtgtctgaat ttcaggctat taagtccctc ccgcttcatc ccaatatagc
     3061 atattatgaa aatgtatacc agtttcagat gtccaatggg ctattcgact atgctgtcat
     3121 gcagtattac aaggatggaa atctgaaaca atttcttcaa agcaggactc ttgactatga
     3181 gagtaaaagg caggttgtag aaggcttgtt gcagggagtt attttcctgc acgaaaacaa
     3241 catcatccat agagatatca aaccgggcaa cgtcatgatt gccatacatc ctcaaggaat
     3301 gatcataccc aagatcacag atttcgggct cagccgaatc aaggatggag acatttcatt
     3361 tatgacagga agcattggcg gggggactct ggaatattcc tctcctgaac aattatatgg
     3421 taagcccatc agtttcaata cagatctttg gtcattcggc gtaattgtct ttgaagtcat
     3481 gaccggcatg aagccatttt atgatgatat gtcgggcaat acagaggcgc agagattgaa
     3541 gctttatcag aatataacaa ccggcaatat ctccgaaaaa atcaatacta ttccaacgcc
     3601 atacaaggaa attattgaac tgtgccttga agctgacgta aggaaaaggg tcgataaagg
     3661 aaacatcctg ttgaattatt tgaggaaata tgataaagtc ggtgccacaa ccggtgatat
     3721 ggatgaggaa acctatgtga tgaccaaagc cgaaagagag gagtatgaac acatccggga
     3781 cagccatgat atcaatgact tgagaatgtt tgtgggaaaa tttccgaata gtatatttta
     3841 tggcgaaatc tctgcaagaa taagttctat ccagggaaac aaacataacg ggaatcagca
     3901 tccacaatcc ggattacata aaagtaccga caaaagagaa cattcaaaag acactttaac
     3961 cccgcatcaa tccaattcta tggctcacat ttctctaaag gacagaataa atcgtcttct
     4021 tgcccgatat cgcaggtata gttatcgcga ttttcgtaca tccaatacta agtttctgta
     4081 tctgattccc attttattct tctcatttat tgtcggatac tgggcctatt cccatttttc
     4141 caaagtggaa atatattcag aaaaaggcaa gtatgggctt caatcccagt taaaagggat
     4201 tatatatgaa gcggtgtttg acaccatcat ccctttggaa aatggtcatt ttagggtaat
     4261 acaattcaat aaaacattca tcgttgacgg aatgggcaat tctcaggtgg atgatacaaa
     4321 atgatgtttt ttttaataaa ataaatttgc tttcaattgc ggttggtact ctattacaga
     4381 aaagacaata gacttagaat gaaattgcaa agtttttttt actgccaata atgaaagtat
     4441 cctgcttgga gaacacaatg atttttaccg cccgccaatt gacttttttg tatttaccag
     4501 ttttgtaaat caatccattg actttttaat gtcacactca gacaacacga cttctgactt
     4561 atccctggaa acaaacagac tgctgatcag acccatacag gagaccgacg ccgcgtttat
     4621 gcttatcctg atgaataccc ggggatggtt agattatatc ggtgatcggc atatcacttc
     4681 gttattgctg gcaaaaaaat acattgccgg gctactccgg gagggcaaaa gccattattt
     4741 cgtggtttcg gaaaaagatt ccggtaaaga ggtcggtgta ttatccttgg tacaacggga
     4801 ttatctggag tatgtagacc tgggttttgc ttttcttcca cagtttcatg gaaaaggaat
     4861 ggcatatgaa gcagcgtcct ccctgatggc cagagtgaag gacattaatc aaactatcct
     4921 tgctgtgact acacctgata atacggcctc tcaggcatta atagaaaaat tgaatttttc
     4981 ttttttgaaa accatcttta gacacgatca acccctcctt ttgtacatac atactacaaa
     5041 ttaatcgatg gggtattgaa cagttgcctg cctgctgaac aaataagccg gcaataacac
     5101 ggttagtatc gcatgtcatt ttccgaccct tgttatacca agatgattta tataatattc
     5161 cataaatttt tggtatactt gtcccgagag ctaagcgatt cggggatgcc gaaggtacag
     5221 tcggtattat atactttttt ccattactcc ggagagtcct tgccttcctt ttccaatttt
     5281 tggcaaaagg ccatgtcggt ctaatttttt attacaaata aaattgggcc ggtctcggcg
     5341 cgccctcttt tgttcttata atttgatgaa tttgagggtt tttgataatc cgccctgttc
     5401 tacagtaata aaataaatgc cgcttcccag gtttccggca tctatcgtct gttcttttat
     5461 tgtttgattt gttccggcct gatgcagcgt tccttttatc agggttttac cacagatatc
     5521 aaaaatccgg taatgacttc ctgcggaata acgcagggtg aactcaccat tggtggggtt
     5581 gggaaacaac caaatggcag gctcctcatt tttagggttc ttaactgagg ataccaggtt
     5641 gatatatttt ttgacactta aacgcttatt ggcaccaaaa tcctgatatg ccacccaggg
     5701 attgccttcg ggatcaatgg ccatggatat ataggatgaa gcattggtaa atccgggttt
     5761 gccaagatat tgccattcta taccatcata catcatcgca ctgaccttgt tatcattttt
     5821 cccatcccta aatgtagcta ttagcttccc tcctagaccg acattcaggt tgcaatagtt
     5881 ggacgcccca ccgctaacat tcgcttttat caattcccat ttgtcgtctt t
//
LOCUS       DAOQMC010000147         5890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5904_length_5890_cov_4.509854, whole genome shotgun sequence.
ACCESSION   DAOQMC010000147 DAOQMC010000000
VERSION     DAOQMC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5904_length_5890_cov_4.509854"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKV31_06810"
     CDS             <1..235
                     /locus_tag="PKV31_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNB92603.1"
                     /translation="STSGMVKPFNGWTNKFIFPPYPFSIADSMLTNFHLPKSSLIIMV
                     CAFGGYDLIMEAYHEAIKKDYNFYSYGDAMLII"
     gene            269..490
                     /locus_tag="PKV31_06815"
     CDS             269..490
                     /locus_tag="PKV31_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNB92604.1"
                     /translation="MYWTLELAHHLEDAPWPATRDELIDFAIRSGAPLEVIENLQEME
                     EEGEMFEGIEDVWPDYPRRDDFLFNEDEY"
     gene            511..1218
                     /gene="cmk"
                     /locus_tag="PKV31_06820"
     CDS             511..1218
                     /gene="cmk"
                     /locus_tag="PKV31_06820"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNB92605.1"
                     /translation="MNIGLFFLIHRVSNKINIAIDGFSGCGKSTLARAMAHELNYIYI
                     DSGAMYRAVTWLMIQKGVNPEDTESVHQVMDEMDLHFKRIDKVNHLYLNGRHLRDEIR
                     TMEVNNLVSQFSTISIVRSKLVQLQKKLAEKKGCVMDGRDIGTVVLKDAELKLFLVCE
                     MDIRVARRKKELEEKNIFTTFESVKENFIERDRIDSTRDDSPLQQASDAILLDNSHLG
                     PDQLLLKAMELAYARLG"
     gene            1220..1843
                     /locus_tag="PKV31_06825"
     CDS             1220..1843
                     /locus_tag="PKV31_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNB92606.1"
                     /translation="MELFIAVLASLFSVVNPIGALPVYTSMTSSYSIKERRRTALHTT
                     MYFTMICIIFFLGGSYILSFFGISIPALRLAGGLVILNSGFSLLNGKVIRAEAINEKM
                     KEEARQKEDISFSPLAMPLLSGPGSISLLISLYALHPSWHEKGIIIGVMFALGLIIYI
                     MLLSAPLIARMLGEAGMKAITRIMGFIVMAIACEYIVGGATSLISKQ"
     gene            1874..2524
                     /gene="upp"
                     /locus_tag="PKV31_06830"
     CDS             1874..2524
                     /gene="upp"
                     /locus_tag="PKV31_06830"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNB92607.1"
                     /translation="MAHNFSLSNSYINQIVYELRHINIQKDRAKFRNNLEKFGILAGY
                     ELSRHFHYSAEEVQTSLGSTIIETLDDSIVIISILRAGIPLQYGLLKLLDNAETGFIA
                     AYRKEHPGGGFDIKMDYITCPDLSGKVVIFTDPMLATGSSLDTAMKMIENYGEPKELH
                     LISVIASEFAVEQIKRLHPSIRMWVGAIDEELTAKSYIVPGLGDAGDLAYGEKLQG"
     gene            complement(2788..3138)
                     /locus_tag="PKV31_06835"
     CDS             complement(2788..3138)
                     /locus_tag="PKV31_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNB92608.1"
                     /translation="MIEENTTEQILTLTPGAIRQLAIIRQEQSIPEDLGLRVGVKGGG
                     CSGFSYELGFDSYKMGDEVIIIDGLEVYMQKSHSIYLKGIEIDWEEGLNNRGFSFKNP
                     NASSTCGCGTSFSA"
     gene            complement(3235..3765)
                     /locus_tag="PKV31_06840"
     CDS             complement(3235..3765)
                     /locus_tag="PKV31_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB92609.1"
                     /translation="MAFLCIALLSYMASYGQGIPVSRDTSQSKAKAIKPVLAPAGKEV
                     IYQGAPVQNKFEISQEDGKIVVSPETMPSFPGGQPALEEFIAANMRYPEEALKAGAEG
                     IVVVKFTIEYDGTAHSPVILLDKVGHGANAETKRLVQLMPKWIPATLSQQPVTVEFTM
                     PVNYNLKKYHRVKGNK"
     gene            complement(3788..4306)
                     /locus_tag="PKV31_06845"
     CDS             complement(3788..4306)
                     /locus_tag="PKV31_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB92610.1"
                     /translation="MANRLNILMGIGCFLCVSVTVIGQNDRIPAKQQDEVKLIEVKSK
                     QQKALNQATPEKNAKEIWWEENQVYTVVEDMPQYPGGTEAMYKFIGEHLVYPAEAKAA
                     GKEGLVVLSFIVEKDGSIHDIEILKDGVGFGAAEAASKVVSSMPVWSPGKQDGKPVRV
                     KITSPFKFILPK"
     gene            complement(4394..4840)
                     /locus_tag="PKV31_06850"
     CDS             complement(4394..4840)
                     /locus_tag="PKV31_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92611.1"
                     /translation="MMICTGLQAQQPETAGGKAKPIKAREVKKAEKAAEVKAAEEVRD
                     EIYDEVDEMPVYKIGNKAFYSLMRQNFEALEREGVYVDKTMTLNFVVGKDGTVRDMVV
                     LDNKGEKVTESKTLRQNMPYWGPGRKQEKAVSTRMQVPIEFILEKN"
     gene            complement(5161..>5890)
                     /locus_tag="PKV31_06855"
     CDS             complement(5161..>5890)
                     /locus_tag="PKV31_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HNB92612.1"
                     /translation="NNYDIAYKSNLPGVTDFQLLWGINDVMNKPFGWTDGVNKLYTTL
                     ANDFVYKDPYNNVIFLDNHDLSRAYSVYGEDLKKYKMALTWLLTCRGIPQLYYGNEVL
                     MKGTTYPNDGYVRLDFPGGWPGDSVNKFTREGRSADENEVWDLISRLAHYRRNSVALT
                     TGRLTQFVPEDGVYVYFRHAGDERIMCVMNSMDTDKQIEVARFNELTDGYRSAKDVLG
                     GSIISLQGKLNIPARTMVVWELLK"
BASE COUNT         1639 a         1388 c         1147 g         1716 t
ORIGIN      
        1 ttctacctcg ggaatggtaa aaccgtttaa cggatggacc aacaagttta tcttcccgcc
       61 atatcctttc agtattgcgg atagtatgct caccaatttc cacttgccaa agtccagcct
      121 gatcatcatg gtatgtgcat ttggaggata tgatctgatt atggaagcct atcatgaagc
      181 catcaagaaa gattataatt tttattccta cggtgacgca atgcttatta tttaatatat
      241 taatcaacaa tttgtaaatc cttaaactat gtattggaca ctggaattag cacaccatct
      301 tgaagatgcc ccttggcctg ccactcgtga tgagctgatc gactttgcta ttcgttcagg
      361 tgcacctttg gaagtgattg agaatctgca ggaaatggaa gaagaaggcg aaatgtttga
      421 aggaatcgaa gacgtttggc ccgattatcc gcgaagggat gatttccttt ttaatgaaga
      481 cgaatattga tgcatgataa aataagccga atgaatatcg gcttattttt tttaatccac
      541 cgggtgagca ataagataaa tatagcaatt gacggttttt ctggctgcgg caaaagcaca
      601 cttgcccgtg ccatggctca tgaattaaac tatatttata tcgattccgg tgctatgtat
      661 cgagctgtaa catggttgat gatacagaaa ggtgtaaacc ctgaagacac ggaatcagtt
      721 catcaagtca tggatgaaat ggatctccac ttcaaacgta tcgataaggt caaccatctc
      781 tacctgaacg gccgacacct cagggatgaa atacgaacaa tggaagtcaa taacctggtc
      841 agtcagtttt ccaccatctc aatcgtaaga agtaagctcg tgcaacttca gaaaaaactg
      901 gcagaaaaga aaggctgtgt catggatggc agggatatcg gcacagttgt tctcaaggat
      961 gccgagttga agttattcct tgtttgcgaa atggatattc gcgtggcaag gagaaaaaaa
     1021 gagctggagg aaaagaatat ttttactaca tttgaatcgg taaaagaaaa ttttattgaa
     1081 agggatagaa tagattcgac ccgggatgat agtcccttac aacaagcatc tgatgctatt
     1141 ttgctggaca attcccatct cggtccggat caacttctgc tcaaagctat ggaattggca
     1201 tatgccagat taggttgata tggaattatt cattgcagta ctggcttcat tgttttcggt
     1261 ggtcaatccc atcggtgctt taccggtata tacttcaatg acgagcagtt actcgatcaa
     1321 ggaaagacgg cgtaccgcac tgcatacgac catgtatttc accatgatct gcatcatatt
     1381 cttcctgggc ggatcctata tcctgagttt ctttggcata tccattccgg ccttgaggct
     1441 tgcgggtggc ttggttatac taaattccgg tttcagttta ttgaatggca aggtgattcg
     1501 tgctgaagcc atcaacgaaa aaatgaaaga agaagcccgc caaaaggagg acatcagctt
     1561 cagcccgctc gccatgccgt tactttcagg ccctggctcc atatccttgt tgataagtct
     1621 gtatgcgctc catcccagct ggcatgaaaa aggtataatc atcggagtca tgtttgctct
     1681 cggcctgatt atctacatca tgttactcag tgcacccctg atagccagaa tgcttggtga
     1741 ggcaggcatg aaggcaatca caagaattat gggctttatc gtcatggcca tagcttgtga
     1801 atacattgtc ggtggagcca caagcctgat ttcaaaacaa tgatctatct ttattcttat
     1861 aaattccttt gctatggctc ataatttctc tctaagcaat tcgtatatca atcaaatagt
     1921 gtatgaatta aggcatatca acatccagaa agaccgggca aagtttagga ataatctgga
     1981 aaaattcggc atcctggccg gatatgagct ttccagacac tttcattatt ctgcagaaga
     2041 agtacaaacc tctctcggct cgaccatcat cgagacactg gatgattcga tagtcatcat
     2101 ctccatcctg agggcaggta tcccactgca atacggactt ctcaaattgt tggataatgc
     2161 tgagaccgga tttatagctg cttacaggaa agaacacccg ggtggtggtt ttgatatcaa
     2221 gatggattac attacctgtc ccgacctcag tggcaaagtg gtcatcttta ctgacccgat
     2281 gcttgctacc ggttcttccc tcgataccgc catgaaaatg atcgaaaact atggcgaacc
     2341 taaggagttg cacctgattt ctgtcatcgc ttcggaattc gctgtcgaac aaatcaaaag
     2401 gctacatcca tccatcagga tgtgggtagg agccatcgac gaagaactca ccgccaagtc
     2461 ctatatcgtc ccgggactcg gagatgccgg tgatcttgct tatggagaaa aactccaggg
     2521 ctgatacaaa tccctccaca gtatgccgag cctcgcttgc tgattaaact gaactattct
     2581 gttcctatgt aaaggacgga agaggtaaaa caaaatggac tactaaaata tgaaggtatt
     2641 ttgattttgt gattaaaagt atatatccag gggcgaattc tcttttgttg ccaataattt
     2701 ccacgctaga aatattttac tatcactaaa aacaaaaaac cggaccaaat gacccggctt
     2761 aattttaatt tttttaactg tttcttctta tgctgaaaac gaggtgccac acccacaggt
     2821 gctgctggca ttggggttct taaacgaaaa accacgatta ttaaggcctt cctcccagtc
     2881 tatctcaata cctttcagat agatactatg cgatttctgc atatacactt ccaatccatc
     2941 gattataata acctcatcgc ccattttata ggaatcaaat cccagctcat aactaaaacc
     3001 ggaacatcca ccacctttga cacccacccg gagccccaaa tcttccggga tactttgttc
     3061 ctgccttata atggctagtt gccggatcgc tcccggagta agggtcagga tttgttcggt
     3121 tgtattctcc tctatcatcg taaaattaga ttttgataat tatttaatgt ttttccacaa
     3181 tcaaccatga ttcaacaaca tatttctcca atcatagttc aatacccggt cagtttactt
     3241 gttacctttg accctatgat acttctttag attgtaattg acaggcatgg taaactccac
     3301 agtcacaggt tgttgggaaa gggttgccgg aatccatttt ggcatcagtt gcaccagtcg
     3361 ttttgtttcg gcatttgcac cgtggcccac cttatccagc aatatcacag gtgaatgtgc
     3421 cgtaccatca tattcaatcg taaactttac tactacgatt ccctccgcac cggctttaag
     3481 cgcttcttcc ggatacctca tattcgcggc aataaactct tccaaagcag gctgtcctcc
     3541 cgggaaagaa ggcattgttt ccggggaaac tactatttta ccatcctcct gagatatttc
     3601 aaatttattc tgaacaggtg caccttgata gattacttcc ttaccggctg gcgccaaaac
     3661 cggcttaata gctttagcct tactttggga tgtatctctg ctaacaggaa taccttgacc
     3721 ataacttgcc atatatgaca gtaaggcaat gcacaaaaaa gccattctcc tcatcaaaag
     3781 attcaaatta tttcggcaat ataaacttaa aaggactggt aattttgact cgtacgggtt
     3841 tgccgtcttg tttgcccgga gaccatacag gcatggaact tacgactttt gaggcagcct
     3901 cagctgcacc aaagccaacc ccgtccttca gaatttcaat atcatgaata cttccatctt
     3961 tttccacaat aaaggaaaga acgactaacc cttccttgcc ggcagccttg gcttcagcag
     4021 gatataccag atgctcaccg ataaatttgt acatggcttc tgttccaccg ggatattgag
     4081 gcatgtcttc cacgacggta tatacctgat tctcctccca ccagatttcc tttgcattct
     4141 tttcaggagt cgcctggttc aatgcctttt gttgcttcga tttaacttca ataagtttga
     4201 cttcgtcctg ctgcttcgcg ggaatccggt cattctgtcc aattactgtc acagaaacac
     4261 acaaaaagca accaatcccc atcagtatat tcaatctatt tgccatatga ttaattttat
     4321 tttgatatat aatatgcact cagattggaa tcccaaaaaa taaatgttat tcattcggat
     4381 ttcctaaagt atcctaattt ttttccagaa taaattcgat aggtacttgc atccttgtag
     4441 aaacagcctt ttcctgcttc ctacctggtc cccaatacgg catattctgt ctaagcgtct
     4501 ttgactcggt gactttttca cccttattgt ccagcacaac catatccctg actgtaccat
     4561 cttttccaac aacaaaattg agggtcattg ttttgtccac atacacccct tcgcgctcca
     4621 atgcttcaaa attctgcctc atcagactat agaaggcctt attgccaatt ttgtataccg
     4681 gcatctcatc tacctcatca tagatctcgt ctcgtacctc ttcggccgcc ttaacttcag
     4741 ccgctttttc agctttttta acttcccttg ctttgatggg tttcgccttg ccacctgcag
     4801 tctccggttg ttgcgcctga agaccggtac agatcatcaa aaaaaatagt attaagatat
     4861 tgaacttcat attcttacgg ttgaaaggta aaggtacgaa taagcaaaaa gttataataa
     4921 tatttaacca aaaattaagc aataaccacc aacgcttgat tatgctgtgc ttgattaggg
     4981 cgtgcccctg tcgggtcggg tcatccactg tacttcgcta ctcactcctg agagaggaag
     5041 tgggtccagg gattgaaccg acttcgagta acgatatttt cagggaagaa ggccgctcgt
     5101 gccgtttcta cccctcacgc atacggcacc agtggagcga catgacctaa ttccaatccc
     5161 ctacttcaag agttcccaca ctaccatggt tcgtgcaggg atattcaatt tgccttgcag
     5221 tgagataatc gatcctccca aaacatcttt ggccgacctg taaccatcag tcaactcatt
     5281 aaagcgggct acctctatct gtttgtctgt atccatacta ttcatcacac acatgattcg
     5341 ctcatcaccg gcgtgcctaa aatatacata gacgccgtct tcgggtacga attgtgtcaa
     5401 tctgcctgta gtcagggcca cggaattcct cctgtaatgg gcaagccgac tgattaaatc
     5461 ccatacttca ttctcatcgg cactcctacc ctctcttgta aatttattga cagaatctcc
     5521 cggccatccc cccggaaaat caagcctgac ataaccatca ttgggatagg ttgttccttt
     5581 cataagcacc tcgttaccat aatacagctg agggatgccc cggcaagtca acaaccaggt
     5641 cagtgccatc ttgtacttct tcagatcctc tccatatacc gaataagcgc ggctcaggtc
     5701 gtgattgtcc agaaaaatga cattattgta tggatccttg tatacaaagt cattcgccag
     5761 tgtagtatac aatttattca ctccgtccgt ccagccaaat ggcttgttca tcacatcatt
     5821 gatgccccaa agaagttgaa aatcagttac gcccggcaag ttacttttat aggcaatatc
     5881 ataattgttg
//
LOCUS       DAOQMC010000148         5854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_5956_length_5854_cov_5.945680, whole genome shotgun sequence.
ACCESSION   DAOQMC010000148 DAOQMC010000000
VERSION     DAOQMC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5956_length_5854_cov_5.945680"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKV31_06860"
     CDS             <1..307
                     /locus_tag="PKV31_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92613.1"
                     /translation="LMAIRVANKADLKGTYNFAHKIGSLEVGKGIGVFDGSSTDLLTY
                     ATGLDLLEYVDDSKVIITEHKNGRISGTFQFRMEHIISKQGFNAVKEGTFKNFKIKQ"
     gene            445..1197
                     /locus_tag="PKV31_06865"
     CDS             445..1197
                     /locus_tag="PKV31_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNB92614.1"
                     /translation="MQGQKILVLLVSLAFWGCNSDDSMTKNNTTQISKQDFLLGRFDP
                     QKHPDFVLIDQKYADKEGMMMQGEAYSAFLKMAHAGELAGHSFCILSATRNFFYQKYL
                     WENKWYGIFPIQGNIDACTSFQDEVYRARKIMEYSAMPGSSRHHWGTDIDINALDNDY
                     FEQGEGLAWYRWMTENAGAYGFCQPYTAGRKYGYKEEKWHWTYLPLSKQYTEEIRRTI
                     TDDMIDGFAGAEHSHQLEIVDHYILGINKACR"
     gene            complement(1359..3536)
                     /locus_tag="PKV31_06870"
     CDS             complement(1359..3536)
                     /locus_tag="PKV31_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92615.1"
                     /translation="MRFLQTVILQIIIMLPALAQHHQDTVKKHTLDPVIIGGMKSTSD
                     IRRLNKVEGTYIISGKKNEVIELNNQNNGLAEKYCRQIFAKIPGIFVYDMDGTGNQVN
                     ISTRGLDPHRGWEFNIRKDGIITNTDLYGYPASHYNVPYEAVERIELVRGTSAQQYGG
                     QFGGMLNYITKQPDSRKIGFESINTVGSFGLLSTYNSISGTIGKFRYAAWMNKKAVDG
                     YRRFNDSKYNAESLALYYDFSENIKLKLEYTHSNYITHLAGALTDKEFKEDPTQATRT
                     RNYYQPDIHIPNLTFDWKISDHTSLRYTSNAILGARNSVMFDKPANVRDTIVTATGQY
                     NNRQVDIDNYHTFLNELRMVHEYQFFNLKNTITSGIQVTNNDLHRRQQGVGTTGTDYT
                     LTLVKPGWGRDLHFKTGNIAVFVENSWAITDRLSFNTAARMEYGVSRLSGKINLYPED
                     ELPNTIDHKFPIFGASFQYNLDKSNIYGGWSQAYRPVILKDIVPGSLYEKADKNLRDA
                     FGYNAELGYRGSAGWLQWDIDAFYLLYRHRLGTFAQVDADNNLIIYRTNIGDSHTKGI
                     EAFIQGDWTLGKFMTLSLFNATSLMDARYKNATIRSGSENISVDGNKVESVPTVITRS
                     GATLKYNRWTYCIKYSYTAESFADALNTITPSANGATGIVPAYSLVDMNLSVRVNDKL
                     RLQANVNNVLDESYFTKRPQFYPGPGIWPSDGRSFSVTASIKI"
     gene            complement(3872..5836)
                     /locus_tag="PKV31_06875"
     CDS             complement(3872..5836)
                     /locus_tag="PKV31_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNB92616.1"
                     /translation="MPLKSVLFTVFCFLYQFSYGQHIARGDFRNVLLLEAPILHEKDV
                     AVAVFSDLGAWHAYRFHRDQNGKIGFSGPLIMGMEGRWVGKYISRPRIFIDGTEKAFL
                     PDQITGTAYPGMMEIKYTSSDIAIHQRLIFVSNRQSMILTEITNLTHKKLKVEPCLEG
                     KFLESGRLTKRDREIYFDVGTTPGFTLRLDRDASIRILDHQYVIVFDTQSMKPGEKLS
                     YVESHIYSPVDSEHIHTINTESATFQQSEATNEYRWNNYLNTYFANAGELPAEKRRLA
                     VKAIITLITNWRSAAGDLHHDGLFPSVSYQGFYGFWSWDSWKQAVGLALFNPALAKSN
                     ILSMFDYMDDFGMVADCIYLDKSENNYRDTKPPLAAWAVESIFTRSGDTSFVQSIYPR
                     LLKYHHWWYKNRDHDGNGLCEFGSTDGTRLAAAWESGMDNAVRFDSANMVRNNTHAWS
                     LDQESVDLNAFLYAEKLCLANLADIIGLNEDAQQLRKESRWLITKIRQYLFDSTTGYF
                     YDKKLNEHHLIKVAGPEGWLPLWARCADDEQARSVVHVVLDTARFNTFLPFPTLDASH
                     PEFNPAKGYWRGPVWTDQLYFGLKGLRNYGYWNDANKLQEKFFNHAEGLTTNKPLYEN
                     YHPLTGVGLNAMNFSWTAAHILMLLVEDSP"
BASE COUNT         1571 a         1284 c         1349 g         1650 t
ORIGIN      
        1 actgatggct attcgagtgg ccaacaaggc cgacctgaaa ggcacatata attttgccca
       61 taaaattggt agcctcgaag tcggaaaggg catcggagta tttgatggga gttcgacaga
      121 tttgcttact tatgccacag gacttgactt acttgagtat gttgatgact cgaaggttat
      181 tattaccgag cataaaaacg ggcgcatttc aggaacattc cagttcagga tggagcatat
      241 catttcaaaa cagggtttta atgcggtcaa agagggtaca tttaaaaatt tcaaaatcaa
      301 gcaataagca aaggggcgaa aagaaatgat caaagatcat cagtacccgc atccggggca
      361 aaggtttatt ctatggatag tttcctgagt tactatattt cttgatactt tggttagaat
      421 ataataaagg ctaaaagacc atcgatgcag ggacaaaaaa tactggtatt gcttgtatca
      481 ttggcttttt ggggttgtaa ttccgatgat tcaatgacaa agaataatac aacacagatc
      541 agcaaacagg actttctgtt gggtcgattt gacccgcaga aacatccgga ttttgttttg
      601 atagatcaga agtatgccga caaagagggc atgatgatgc aaggcgaggc ttacagcgca
      661 tttctgaaga tggctcatgc cggcgagctt gccggacact ctttctgtat cctttcagca
      721 acccgtaatt tcttttatca gaaatatctc tgggaaaaca agtggtatgg tatctttccg
      781 atccaaggca acatcgatgc atgtacatcc tttcaggatg aagtctatcg tgccaggaag
      841 atcatggaat attcagccat gccgggttct tccagacacc actggggtac ggatattgac
      901 atcaacgcac tcgacaatga ttattttgaa caaggagagg gacttgcctg gtatcggtgg
      961 atgacggaaa atgccggtgc ctacggattt tgccagccct atacggcggg tcgcaaatac
     1021 ggctacaagg aagaaaagtg gcactggaca tatcttccac tttccaaaca atatacagaa
     1081 gagattcgta gaacgatcac cgatgatatg attgatggct ttgcgggcgc tgagcatagt
     1141 catcaattgg agatcgtcga tcattatatt ctgggaataa ataaagcgtg ccgataacgt
     1201 ccgcatgaat catttactaa aaaacgacac cactcaggtg ctgctttcat ttaataccgg
     1261 cctccggtaa tctttttttg accgaatatt aaatgaaagc ggcaaaaatg ctacgtcccg
     1321 gctaattgta cttcgtccgg ccgagatcgg acataggatt atatttttat acttgcagta
     1381 accgaaaatg atctcccgtc agaaggccaa atacccgggc cgggataaaa ctgaggcctc
     1441 ttggtaaagt aggattcatc gaggacgttg ttgacattgg cctggagtcg taatttatca
     1501 ttgacacgga cactgagatt catatccacc agggaataag ccggaacaat acctgtagcg
     1561 ccattggccg atggagtgat tgtgttgagc gcatcagcaa agctctcagc ggtatagctg
     1621 tacttgatac aatatgtcca acggttatat ttcagggtgg cgccactgcg cgttattaca
     1681 gtcgggacac tttccacttt attaccgtct actgagatat tttcgctacc ggatcggatc
     1741 gttgcattct tgtatcgggc atccattagg gacgttgcat tgaacaaaga caatgtcata
     1801 aatttgccca gggtccagtc tccctgaata aatgcttcta taccttttgt gtgggaatca
     1861 cctatgttgg ttctatagat aatcagatta ttatctgcat ccacttgagc aaatgttccc
     1921 aaccgatgcc tgtacaacag gtagaaggcg tcaatgtccc actggagcca tccggccgaa
     1981 cccctatatc cgagttcagc attatatccg aaagcgtcac gcaggttttt gtctgccttt
     2041 tcatataggg atccgggaac gatatctttc agaatgaccg gccgataggc ttgggaccag
     2101 ccgccataga tattgctttt atcgagattg tattgaaatg atgctccgaa aatcgggaat
     2161 ttgtgatcaa ttgtattggg cagctcatcc tccggatata aattgatctt gccggacaat
     2221 cgggacacac catattccat ccttgctgcc gtgttgaagg agaggcgatc ggttatggcc
     2281 cagctgtttt cgacaaatac tgcaatattg ccggtcttga aatgcaggtc ccgtccccag
     2341 cccggcttga ccagggtaag tgtataatcg gttcctgtag tgccgacgcc ctgttgtcga
     2401 cgatggaggt cattgtttgt gacctgtatg ccggaagtaa tagtattctt cagattaaaa
     2461 aattgatatt cgtgcaccat cctcagctca ttcaagaaag tatggtaatt gtcaatatct
     2521 acctgtcggt tgttgtattg tccggttgct gtcacgatgg tgtctctcac attggccggt
     2581 ttgtcaaaca tgacactgtt tctggcgccc aatatggcat tggaagtata gcggagactc
     2641 gtatggtcgc ttattttcca atcgaaggtc aagttgggga tatggatatc cggttgatag
     2701 taattgcgag tcctggtagc ctgggtggga tcctccttaa actccttgtc cgtcaatgct
     2761 cctgcaagat gtgtaatata gttggaatgc gtgtattcca gctttagctt tatattctca
     2821 ctgaagtcgt aatacaaggc caaagactct gcgttatatt tggagtcgtt gaacctacga
     2881 taaccgtcca ccgccttttt attcatccag gcagcatatc gaaacttacc gattgtccca
     2941 ctgatggaat tataggtact caatagaccg aatgatccta cggtattgat ggattcaaat
     3001 ccaatcttgc ggctgtcagg ctgtttggtt atataattga gcatgccgcc aaattgcccg
     3061 ccatattgtt gggcagaagt gccgcgcacc agctcgattc tttccaccgc ctcataaggc
     3121 acattgtaat ggctcgcagg ataaccatac aagtcagtgt tggtgatgat gccgtccttg
     3181 cggatgttga actcccagcc acggtgaggg tccagtcctc gagtggagat attgacctga
     3241 ttgccggtgc cgtccatatc atagacaaaa atcccgggta tcttggcaaa tatttgacga
     3301 caatattttt ctgccaggcc attgttctga ttattcagtt cgatgacttc gttctttttc
     3361 ccggaaatga tataggtacc ttcgacttta ttgagtcggc gtatgtcact tgttgacttc
     3421 attcctccaa tgatgacagg atcgagtgta tgcttcttga ccgtatcttg atgatgttgt
     3481 gcaagagcgg gaagcatgat tatgatttga aggattacgg tttgtaaaaa tctcatgaag
     3541 tgaaaaatct gattgattgc aaaagtaagg ttgaccttcc cggcaaaaaa acgcaccggt
     3601 taaatttaat ttatttttaa tggtatcccc aatttcagat tcgtatttat ccggtcaaat
     3661 aggtgtctca aaagcagatt ggttgatgcc acataaatat caatcaacaa tgacagtaac
     3721 tttggattgg gggagatcag tgattgattc aaaaggaagt gattattgaa atatgaagac
     3781 ggagaaccat ctgcgtcagt gaattgtggg aaaatgattt tcctgccgcc ttctctttgt
     3841 cgctgaaaga agccattccc ggcctgatgc ctcaagggct gtcttccacc agcagcatta
     3901 aaatatgtgc agctgtccaa ctgaaattca tggcattaag acccactccg gtcaagggat
     3961 ggtagttttc atacaagggc ttgttggtcg tcaaaccttc tgcatggttg aaaaacttct
     4021 cttgcagttt atttgcgtca ttccaatacc cataattacg caatcctttt aacccgaaat
     4081 acaattggtc ggtccatacg ggacctcgcc agtatccttt tgccggattg aattccggat
     4141 gcgaagcatc gagggttgga aaaggaagaa aggtattgaa tcttgctgtg tccagcacaa
     4201 catggaccac agatcgcgcc tgctcgtcgt cggcgcatcg tgcccataaa gggagccagc
     4261 cttccggtcc ggcaaccttt atcaagtggt gttcgttcaa ttttttatca taaaaatagc
     4321 ctgtcgtact gtcaaagaga tattgtctga tcttagtgat aagccacctg ctttccttcc
     4381 ttaattgttg agcatcttcg ttcaggccga ttatatcagc aagattggca agacacaatt
     4441 tttcagcgta tagaaaagca ttgaggtcca cactttcctg atcgagtgac catgcgtgtg
     4501 tgttgttccg gaccatgtta gcggaatcaa agcgcaccgc attgtccata ccgctctccc
     4561 aggctgcagc gagccttgtc ccatccgtcg atccgaactc acaaaggcca ttgccatcgt
     4621 gatcccggtt tttgtaccac cagtgatgat atttcaaaag tcggggatag atcgactgta
     4681 caaatgaagt gtcaccggac ctcgtgaata ttgactccac agcccatgct gcgagtggcg
     4741 gctttgtatc ccggtaatta ttttccgact tatccagata tatgcaatcg gctaccattc
     4801 cgaaatcatc catataatcg aacatgctca gaatattgct ttttgccaga gccgggttaa
     4861 acaaggcaag cccgaccgcc tgtttccagg agtcccaact ccagaaacca tagaatccct
     4921 gataggaaac agatggaaag aggccatcgt ggtgcaggtc tcccgctgcc gaccgccagt
     4981 tggttatcaa tgtgattatg gccttgaccg ccagacgcct cttttcagct ggcagttctc
     5041 ctgcatttgc aaaataggtg ttcaggtagt tattccagcg atactcattg gttgcttcgg
     5101 actgttgaaa tgtcgctgat tctgtattta tcgtgtgaat gtgttcacta tcaacagggc
     5161 tgtaaatatg agattcgaca taggaaagtt tttctcccgg cttcatggat tgcgtatcga
     5221 aaacaatgac atattgatgg tccaggatcc gtatggaggc gtcccgatcc aaacggagtg
     5281 taaatccggg tgtagtgccg acgtcaaaat atatttcgcg gtctcttttt gtcaagcgcc
     5341 ccgattcaag gaactttcct tccagacacg gttccacttt caacttttta tgggtcaggt
     5401 tagtgatttc agttaagatc atcgattggc ggttgctgac aaatatcaat cgctgatgga
     5461 ttgcgatatc agatgaggta tattttatct ccatcatacc gggataggcg gtccctgtta
     5521 tttgatccgg taaaaatgcc ttttctgtac catcgatgaa tatccggggc cgggagatgt
     5581 atttgccgac ccatcgaccc tccatgccca taatcaaagg cccggaaaaa cctatctttc
     5641 cattctgatc ccgatgaaaa cgataggcat gccatgcccc cagatcactg aaaacagcca
     5701 ctgcaacatc tttttcatgc aggatagggg cctcgagtag caagacattt cggaagtccc
     5761 ctctggctat atgctgtccg tatgagaatt gatataggaa acaaaaaacg gtgaagagaa
     5821 ctgatttcaa aggcataaac taaagaatat aaaa
//
LOCUS       DAOQMC010000149         5819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6010_length_5819_cov_5.718425, whole genome shotgun sequence.
ACCESSION   DAOQMC010000149 DAOQMC010000000
VERSION     DAOQMC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6010_length_5819_cov_5.718425"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PKV31_06880"
     CDS             complement(<1..857)
                     /locus_tag="PKV31_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB92617.1"
                     /translation="MPSHDQICRDIIRERRVCILIPTYNNAGTLSQVLEGCLFYSENI
                     IVVNDGSTDSTDTILKRFDNITVFHHAVNQGKGMAIKTGFDAAMAAGYQYVVTIDSDG
                     QHLPADLPYLINGLYNHPDSIIIGSRNMNRAGIPGKSSFGNKFSNFWFRFDTGIDLPD
                     TQSGYRIYPLESLKGIRLLTWKFEFEVEILVKSAWRNIPIIPVPVRVYYPPGDIRVSH
                     FRPFRDFTRISILNTIFFIQALLFYIPLRWWRQLSNIGFRKSLMLALHNPLESVSRRS
                     AAIGFGVFMG"
     gene            complement(861..1235)
                     /locus_tag="PKV31_06885"
     CDS             complement(861..1235)
                     /locus_tag="PKV31_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92618.1"
                     /translation="MKDSLLLYDYTRVNGNHTQNVEYSVRTNPDNPIFKGHFPGRPVL
                     PGVMILRLFTLCASDVCGKDLMMQRTVQSKFLNFVDPQVHSELTVHLSISEDEEGYNV
                     GAELKNSTTSFTKAILRLIPAP"
     gene            1317..2390
                     /locus_tag="PKV31_06890"
     CDS             1317..2390
                     /locus_tag="PKV31_06890"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HNB92619.1"
                     /translation="MQYLILILAAFWTSCHDKDNEPKAQVNQSSENIIISLPAHQEEI
                     VLAGGCFWCLDAVYQDLYGVIKVRSGYSNGDIKNPTYEQVCTGLTGHAEVVKLTYDTT
                     AIKLKDILDVFWTVHDPTTLNRQGNDVGPQYRSGIYFFNGTQRAIAQHSMNTTAKEIW
                     GNNITTEILPVKNFFPAEDYHQNYYNNNRNQSYCRFIITPKVEKFREKFSSKLISNQK
                     TTLMEKKAKWNTLTKEEERIIVHKGTEYPGTGKYYKFTGDGIYECRRCNAPLYDSKDK
                     FPSECGWPSFDDEIPGAVERVPDADGRRTEILCKHCGAHLGHVFEGERLTLKNVRHCV
                     NSVSLQFLTREEFEKQQAELNRE"
     gene            complement(2825..3214)
                     /locus_tag="PKV31_06895"
     CDS             complement(2825..3214)
                     /locus_tag="PKV31_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92620.1"
                     /translation="MKKYAFIFSVLIITVLSLSSFDIAKWKIIGQKEAAYGVDRDVLY
                     VKGNNIYTALKLKVVDAGLNMIDMDVYFENGQKEHVPLQKNFHAGEESRVIDLPGNAR
                     RIDKITFLYDTKGIRKGKANIIVFGRR"
     gene            3693..4613
                     /locus_tag="PKV31_06900"
     CDS             3693..4613
                     /locus_tag="PKV31_06900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNB92621.1"
                     /translation="MTKDDELPYYRNITFWLRWIIILLGMIFLGTLAGTIATGMVSEA
                     TGVEIGIFNFESLDDSGRDRLAWIYRLVIFNQHLMVFIIPALVTMYLFRRQTGHSVFH
                     FVPEVNWNATLMGVLFLLSAYPLVQYSSELNKLIPLPDVLHNMEDQTEKLVRLWLDND
                     SLWILAVNVILIALVPAISEELIFRGFLQTLLSQLVTNKHIAVWCTAIVFSAIHMQFE
                     GFFPRVILGLVLGYLMLWSGNIAYPMIAHFANNAVQVILQFFVRHDPSRMSDMDNYKV
                     PIWLVLISTIILMLLGTVFQSYFVKNKHST"
     gene            4610..5434
                     /locus_tag="PKV31_06905"
     CDS             4610..5434
                     /locus_tag="PKV31_06905"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNB92622.1"
                     /translation="MIQFKDISTRLYTAIVFTAFVLAGLMISKWTYFALLFIISVLCG
                     YELTMIISGLKRKLRFFISLTFSFVPVLMGMVIREDVLNVKDSLLLGIIACWLLFLLA
                     SELLSVQSNPVEKTARSMFSFIYVGIPSLLAYELAFKDGSFSYLPLLGVIVLIWINDT
                     GAYLVGSAIGKKPLMPSVSPKKTVEGTLGGIIATIALAWPLSMINDAFTYPQWLIVAT
                     ITGVSGTIGDLVESKLKRHYGIKDTGTYLPGHGGFLDRFDSFLFVVSFVYLYTQLI"
     gene            5441..>5819
                     /locus_tag="PKV31_06910"
     CDS             5441..>5819
                     /locus_tag="PKV31_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNB92623.1"
                     /translation="MFPIHREGRVTILIAILFSILIWWIFNSFLQELMWLGYALIIFV
                     LVMVLQFFRNPRRQVPAIDEDIVIAPADGKVVVIEKVQEEEVLKCKCIQVSIFMSPLN
                     VHVNRNPLSGIITHNIYHPGKYLT"
BASE COUNT         1615 a         1220 c         1268 g         1716 t
ORIGIN      
        1 cccatgaata ctccaaagcc gatggcggcg gaccggcgac tgacggattc cagtggattg
       61 tgcaacgcca gcatcaaact tttacggaaa ccaatgttgg agagttgtcg ccaccaccgc
      121 aagggaatgt aaaacagcaa ggcctggatg aagaaaatgg tatttaatat actgatacgt
      181 gtgaagtccc ggaatggcct gaaatggctt accctgatat ctcccggagg ataataaacg
      241 cgaacaggta ccggtatgat gggaatatta cgccatgccg actttaccag aatctctact
      301 tcaaattcaa atttccaggt caacagtcgg attcccttga gcgactcaag cggatagata
      361 cggtatccgg actgagtatc cggcagatct attccggtat cgaagcgaaa ccagaaattg
      421 gaaaatttgt tgccgaagga acttttgccc ggaatacccg ccctgttcat gtttcgagat
      481 ccgatgatga tgctgtcggg atggttataa agtccgttga tgaggtacgg caggtcggcg
      541 ggaagatgct gaccgtctga gtcgatggtg acaacatact gataaccggc ggccatcgcc
      601 gcatcgaaac ctgtcttgat ggccatgcct ttgccctggt tgactgcgtg atggaagact
      661 gtgatgttgt cgaagcgttt taatatcgtg tcggtgctgt cagtactgcc gtcattaacc
      721 acaataatat tttccgagta aaaaagacag ccctctagga cctgcgacaa ggtacctgca
      781 ttgttgtagg taggtatcag gatacacacc ctcctttcac ggatgatgtc ccggcatatt
      841 tggtcgtgtg acggcatagg ttaaggggca ggaatcaatc taaggatggc tttggtaaag
      901 gaagttgtac tatttttcaa ttccgctcca acattatatc cctcctcatc ttctgaaata
      961 gacaggtgga cagtcagttc ggaatgaact tgaggatcca caaagttcag gaatttgctc
     1021 tgaaccgtcc gctgcatcat aaggtctttt ccgcaaacat cgctggcaca gagggtaaac
     1081 aaacgcagaa tcattacacc cggcaataca ggcctaccgg gaaagtgacc tttgaagatt
     1141 gggttatcag gatttgtacg gacagaatat tcaacgtttt gggtatgatt tccgtttacc
     1201 ctggtataat cataaagtaa aagactatct ttcatcctta atttgcaaat gagttcatat
     1261 tggttaatat ccacctgaac tcaaccatca atatttttgt ttcaatgaaa aaattcatgc
     1321 agtatctaat tcttattctt gccgcttttt ggacttcttg tcacgacaaa gataacgagc
     1381 ctaaggcaca agtcaaccag tcttctgaaa atatcatcat ttcgttgcct gcacaccaag
     1441 aggaaatcgt gctggcaggt ggttgttttt ggtgtctgga tgctgtatat caagacctgt
     1501 acggtgtgat taaagtacgt tcgggatatt ccaacggcga catcaagaat cctacctacg
     1561 aacaggtctg tactggctta accggccatg ccgaagtggt caaattgact tatgacacca
     1621 ctgccatcaa gcttaaggat atcctggatg ttttctggac ggtacacgac cccactaccc
     1681 tcaatcgaca aggaaatgat gtaggaccgc aatacagaag cggaatctat tttttcaatg
     1741 ggacacaacg tgccatcgcc caacattcca tgaatacaac agcaaaggaa atctggggaa
     1801 ataacatcac tacggaaatt ctacccgtca agaacttttt ccctgctgaa gactatcacc
     1861 agaattacta caacaataac cggaaccaat cctattgcag atttatcatc accccgaagg
     1921 tggaaaaatt cagagaaaag ttcagctcaa aacttatatc aaatcaaaaa actacactga
     1981 tggaaaagaa agctaaatgg aacaccctta ccaaggagga ggaaagaatc atcgttcaca
     2041 agggcactga atatccggga accggaaagt attacaaatt taccggtgat ggcatatatg
     2101 aatgccgtcg ttgcaatgca cccttgtatg actccaaaga caagtttcct tccgaatgtg
     2161 gctggccttc atttgatgat gaaattccgg gtgctgtgga gcgcgtaccg gatgcagatg
     2221 gtcgtaggac ggaaatcctt tgtaaacatt gtggtgccca tctcggccat gtgttcgaag
     2281 gagaacggct gaccctgaaa aatgtgcgtc attgtgttaa cagcgtctca ttgcagtttc
     2341 tgaccagaga ggagttcgaa aaacaacagg cggaactgaa tagagaataa tttcacccgt
     2401 tatggtcaaa gcattgattt atggtgaatg atggatgagg aaaacacaac caatttttag
     2461 cattttttat tttcttaaat catttggatt aatacaggtt ccattaatta attcttggat
     2521 taccaatgcc atttgtggca aatgataaat ggcacggatt agtcaatgac taaaatctcc
     2581 cggttacgat gatcagggat agttttttcg ggagagttgg catgacgagc gattgaccgg
     2641 aattcttaca gtactaaatc agtaccggaa gaaggttccc aggcaacttt tcgaccgcgt
     2701 atgttgatat cgcaatcaaa aaatcgtaag ggaaccgaaa tatatagttc taatagagca
     2761 tgaaattgcc caaaaggtga tttaaaattt gcctggaaaa ataaagctaa agcgtcaggc
     2821 ggacttatct gcgtccaaag actataatat ttgccttgcc ctttctaatg cctttggtgt
     2881 catacagaaa ggtaatttta tctattcttc ttgcattgcc cggcaaatca atgactctgc
     2941 tttcctctcc cgcatggaag tttttttgca ggggcacatg ttctttttgt ccgttttcaa
     3001 aatacacatc catatcaatc atattgagcc ccgcatccac caccttcaac ttgagtgctg
     3061 tgtaaatgtt attacccttt acataaagca cgtccctgtc aacaccataa gcagcctctt
     3121 tttgaccaat gatcttccac ttggcaatat caaaggaaga taatgacaat acggtgatga
     3181 tcaggacgct gaagataaaa gcatattttt tcatgacttt aaaaatttat ttggttattc
     3241 aatgtactcc tttgatataa agttgaggcg aatggtttaa tctggaattg aaaaatttaa
     3301 atgttttata gggtgcgata ttcgaagttt gaatttttca ggcctgtttt tcaagttatt
     3361 aattccctgg tttattgatt agtatgctcc cgcaatttcg ccaatttttc tattacgaat
     3421 caaaaaatgc atggcaaccc tagatatcct tgtgtgtgta caataacaaa cggacaacag
     3481 cccattcctt tgcctacctt taagtcgctc gaggtctccg ggattctctg ctagtgaaac
     3541 agccaatcgg tgttgtataa acacgctcta tctgaatatt ccaaattaat catatctgat
     3601 aaacttaccc ggataatgtt atctttgtct cataatccgg catatatgtc ttgaccggat
     3661 taaacaacca tgaactcatt atatccttat tgatgacaaa ggacgatgaa ttaccatact
     3721 acagaaatat aacgttctgg ctcagatgga taattatact actgggaatg atttttctcg
     3781 ggacactggc cggcaccatt gcgaccggga tggtcagtga ggcgaccggg gtggaaatag
     3841 ggatttttaa ctttgagagc ctggatgatt caggaaggga cagattggcc tggatatacc
     3901 gattggtcat tttcaaccaa catctcatgg tatttatcat tccggcattg gtaaccatgt
     3961 atttattcag gagacaaacc ggccattccg tctttcattt tgttccggaa gtcaactgga
     4021 atgcgacgct gatgggtgtt ttattccttt taagtgccta tcccttagtc caatattctt
     4081 ccgaattgaa taaattgatt cctcttccgg acgtattgca caacatggaa gaccaaaccg
     4141 agaaattggt taggttgtgg ttagataatg attcgttgtg gattcttgcc gtcaatgtaa
     4201 ttctcatagc cctggttccg gctatcagcg aagaattgat tttcagaggg ttccttcaga
     4261 ctctgttgag tcaattggtg accaacaagc atattgctgt atggtgtaca gccatagtat
     4321 tcagcgcaat acacatgcag tttgaagggt tttttcccag agtaattctt ggtctggtac
     4381 ttggttacct gatgctttgg tcgggaaaca ttgcctatcc gatgatcgcc cattttgcca
     4441 acaacgcagt ccaggtcatt cttcagtttt ttgtcagaca tgacccatcc cgtatgagtg
     4501 acatggacaa ctataaggtt ccgatatggc ttgttcttat cagcacaatc atcctgatgc
     4561 tgcttggcac ggtattccag tcttattttg tcaaaaataa acacagtaca tgatacaatt
     4621 caaggatata tccaccagac tatacacggc tattgtattt actgcttttg tattggccgg
     4681 cctgatgatc agtaagtgga catattttgc attattattc atcatcagtg ttttgtgcgg
     4741 gtatgagctt acaatgatca tatccggctt gaagcgaaaa cttcgttttt tcatctctct
     4801 aaccttcagt ttcgtgcctg tgctgatggg catggttatc agggaagatg ttctcaatgt
     4861 taaagatagc ctgttgttgg gcatcattgc gtgctggcta cttttcttgt tggcttcaga
     4921 gctgctctcc gtgcagtcca atccggtgga aaagaccgcc aggagtatgt tcagctttat
     4981 ttatgtcggt attccctcat tgttggccta tgagctcgct tttaaagatg gaagtttcag
     5041 ctatcttcca ctgttgggtg taattgtatt gatctggatc aatgacaccg gtgcatatct
     5101 ggttggatca gctattggca aaaaaccatt aatgccatcg gtttctccta agaaaaccgt
     5161 tgaagggacg cttgggggga ttattgctac tattgctctg gcctggccat tatcgatgat
     5221 caatgatgcg tttacctatc cacaatggtt gatcgtagca accattaccg gagtcagcgg
     5281 aaccataggc gatctggttg aatcaaaact gaagcggcac tacggcatca aggataccgg
     5341 aacttacctt cccggtcatg gaggttttct ggacaggttt gattctttcc tctttgtagt
     5401 ttcctttgtg tatctttaca cccaattaat ctgacaacct atgtttccga ttcacaggga
     5461 aggacgagtg accattttga ttgctattct tttcagcatc ctcatttggt ggatattcaa
     5521 ttcattttta caggaattaa tgtggctggg ttatgcactg ataatctttg tcctggtcat
     5581 ggtgttgcag tttttccgaa acccccggcg acaggttccg gccatcgatg aagacattgt
     5641 gattgctcct gctgatggca aggttgtcgt tatcgaaaaa gtacaggaag aggaggtttt
     5701 gaaatgtaag tgtattcagg taagtatctt catgtcgcca ctcaatgtgc atgtcaatcg
     5761 caatcctttg tccggaataa tcacccacaa tatttatcat cccggtaaat atctgactg
//
LOCUS       DAOQMC010000150         5805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6041_length_5805_cov_4.372696, whole genome shotgun sequence.
ACCESSION   DAOQMC010000150 DAOQMC010000000
VERSION     DAOQMC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6041_length_5805_cov_4.372696"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKV31_06915"
     CDS             <1..721
                     /locus_tag="PKV31_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNB92624.1"
                     /translation="PDEEVEFENAWGICDEDIYKQALKYADKSADKGRPFFQFIMTTS
                     NHRPYTFPEGKIDLPQGERNSAVKYTDYALGQFIEAARKKPWFDNTVFVVVADHCASS
                     AGRWEINIAKHHIPAIFYNLKRKPEVIARLTSQIDLMPTLFGYLNWSYLTELYGKDIR
                     QTKEGDERAFIGNYRTLGMLKNDIFTQIDDRKRTVQFDVNPGDKSMAESKKKNDELRM
                     ETIAFYQTASERFSKGKMKEK"
     gene            1265..1609
                     /locus_tag="PKV31_06920"
     CDS             1265..1609
                     /locus_tag="PKV31_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNB92625.1"
                     /translation="MRLFCIIAWLLFSTVMFSQTFVGTATIYSKKFDGKKTYSGQIFD
                     SRKISVAHPWLPMGTKVEVTNLKNNKKLIATINDRMGKSSGYLLDMSEAAAKMIGLTR
                     RHGVTRIKIKVL"
     gene            1611..3476
                     /locus_tag="PKV31_06925"
     CDS             1611..3476
                     /locus_tag="PKV31_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB92626.1"
                     /translation="MWRLASYLLRLLIYYLALFAVLRGFFLFDAGKYISSVSLTEKFR
                     CFSSAIKLDLAAAAYFVTFTLLLVFIAVLTRKAWINKVITFFHFTMTLIYSGLAIGEN
                     LLYREWQAKVGYESLKHFANISEVMKTPSFAMISYFLLGTGITFIIFRWIYNKWVATS
                     LHFPEYRRTTGFYAAVIPLYLALTAGSVITMRGGLGRFPISVGVSYYSSNSTLNDAAT
                     NVFWHAIYSLLNTSEYKRLQPFIDNPEPGPDFFDIYQSAKDTFPSILNTDKPNIVFFI
                     LESWSADVCNQEGRDSLIAPFFNGLIRQGLYFSNCYASGHVSDQGVPAALGALPVIPS
                     FSLLMSKQQVGELPCITDDLKADGYYTGFLYGGQLDYGNIRGYVFKKGFDEVADVQYF
                     PSSTPKTALGVPDLPLYMELLSKLNSARQPFFYCGYNISTHSPYDVPVDYGLGIGGEN
                     AAFINTIHYGDSSLSVFFKEAEKQPWFDNTLFVFVADHSHESQIIRVREDKDRYRIPL
                     LLYGPVLRPEFCGMDIQKVVSQLDLTGLLLSQLGLEYRGKYPFTRNPLQSDFKEMAFY
                     NFPGGSGIVTPNSWLTRNLNDPKERERIGSDTLKAALYGDIGRYLHKASIYLKDR"
     gene            3590..4591
                     /gene="fbp"
                     /locus_tag="PKV31_06930"
     CDS             3590..4591
                     /gene="fbp"
                     /locus_tag="PKV31_06930"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNB92627.1"
                     /translation="MDKIITLDEFIIHHQLQVDPGAGELTGVLRDIGVAAKIINREVN
                     KAGLVNILGVAGNQNESGDLVQKLDIYANEKIIDCLKNSGDCCGIASEENENFIPFKG
                     VAGKDSKFIVLFDPLDGSSNIDVNVSVGTIFSIYKRLSPMEGECTLEDFLQKGTQQVA
                     AGYVLYGTSTILVYTTGKGVNGFTLDPTIGEFCLSHRDIKIPENGAFYSVNQGYYLKF
                     DLEMRRYIDYCSDNNLGLRYIGSMVSDVHRILFQGGIFLYPNTRKYPKGKLRMQYECN
                     PMAFIVEQCGGKAMNTSLQRIMELDAHELHQRSTIVLGSPLMVDEMKRFVEKYSPMI"
     gene            complement(4808..>5805)
                     /locus_tag="PKV31_06935"
     CDS             complement(4808..>5805)
                     /locus_tag="PKV31_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB92628.1"
                     /translation="LMILVIGISSVLTLWIGGHQVVRGEVTPGNIAEFFIYITLLTWP
                     ITALGWTASQVQQAAASQKRINEFLDADVSFKNGTDQIERINQDIRFEHVSLTYPDTG
                     ITAVNDISFIIKKGSKTAIIGETASGKSTIAYLLMQMYKPTEGRILIGDRDISEFSIE
                     SWRERIGYVPQDSFLFSDSIRNNIGFGLKDESGFDDVERYAVHASVHEDIMTLPQEYE
                     TIVGERGVTLSGGQKQRISIARSLISNPELLILDNSLSAVDTNTELKIASYLNDVDNS
                     ITMIIITQRINNIFNYDRIITMAEGKVIEDGTHDELLKAKGYYYSLYLKLNEGKD"
BASE COUNT         1558 a         1158 c         1320 g         1769 t
ORIGIN      
        1 cccggatgag gaggtggagt ttgaaaacgc ctggggtatc tgtgatgaag atatctataa
       61 gcaagcatta aaatatgctg ataagagtgc agacaagggg cgaccattct tccagtttat
      121 catgactacc tccaaccatc gaccatatac ttttcctgaa ggtaaaattg accttcctca
      181 aggtgagaga aattctgccg tcaaatacac ggattacgcc ttgggtcaat ttatcgaagc
      241 tgccaggaaa aaaccctggt ttgacaacac ggtttttgtg gtagtggccg atcattgtgc
      301 gagcagtgca ggtcggtggg agattaatat cgccaaacac cacatcccgg ctattttcta
      361 taatctcaag cgaaaaccag aagtcattgc tcgcctgact tcccagatcg acctgatgcc
      421 gacattgttt ggctacctca attggagtta cctgacggag ttgtatggta aggatatcag
      481 acagacgaag gagggtgatg aacgagcctt tattggtaat tacaggacac tgggtatgtt
      541 gaaaaacgac atcttcaccc agattgatga caggaagaga accgttcagt ttgatgtcaa
      601 tcccggcgat aaatccatgg ctgagtcgaa aaagaaaaac gatgagttga ggatggagac
      661 aattgctttt tatcagacgg ccagtgagcg gttcagtaaa gggaagatga aggaaaagtg
      721 atttccggat aaagagtgga tgaaaggtta aaacagtaga taacttgccg aatggtgttc
      781 agtatgatga atgtcgtttt cagtttggat ccggtttttt aatgcaggcc ggtatcggat
      841 tgatacagga tgctttggaa ctgttggaat aactaacacc caaaaatttg taagtcttaa
      901 aaaggcggag aattctgttg aattttctaa caaaacaagt aaatttcagg agttgggaat
      961 attattaaca gaatattgtg atattacaat attttgactt taatgatcta ataatcaagc
     1021 gaataaatat gataaatggc tcattattta ctaaaaaagc caatgggagt cccaaaatac
     1081 caaattaata ttttaataac ggtgtgtttg caataaaata ttttgaaaat ctaatgtctt
     1141 ttgaattata ttcttcggaa ttgtgaaaaa tcaggtttga ataagtgttt gattttcttg
     1201 ttattgttgt ctttggagag cgtttttctt tttttattat ctttgcactc caaaatttta
     1261 tcatttgaga ttattttgca ttatagcctg gctgctgttt tctacagtta tgttctcaca
     1321 gacttttgtg gggacagcca ctatctatag caagaaattt gatggcaaga agacctatag
     1381 tggtcagata tttgattcca ggaagatatc ggtggcacat ccctggctgc cgatgggtac
     1441 taaggtggaa gtgaccaatt tgaagaacaa caagaagttg atagcaacca tcaatgacag
     1501 gatgggcaaa tcgagtggct atctgctcga catgagcgaa gcagctgcta aaatgatcgg
     1561 cctgacgcga cgacacggcg tcacccgaat caagatcaaa gttctctgat atgtggcgtt
     1621 tggcctcata tcttctccgg ttattgatct attatctggc attatttgct gtgctgagag
     1681 gtttttttct atttgatgcc gggaaataca tttcatccgt atccctgact gagaagttcc
     1741 ggtgtttctc ttcggcgatc aagctggacc ttgccgccgc tgcctatttt gtgaccttta
     1801 cactcttact ggtttttatt gcagtattga cccgtaaggc gtggatcaat aaggtgatta
     1861 ccttcttcca cttcaccatg actttgatct atagcggcct ggctatcggt gagaatctcc
     1921 tgtaccggga atggcaggca aaggtaggtt atgagtcttt gaagcatttt gccaatattt
     1981 cagaagtcat gaagacccct tccttcgcta tgatttctta ttttctcttg ggcacaggta
     2041 tcacatttat catcttccga tggatataca acaagtgggt ggccacgtct ttacattttc
     2101 cggaatatag gcgcacaact ggtttctatg ctgctgtcat cccgttatat ctcgcattga
     2161 ccgcagggtc ggtcattacc atgaggggag gtctgggtcg ttttccgatt agtgtcggtg
     2221 tttcctatta ttcttccaat agtacgctca acgatgctgc taccaatgtt ttttggcacg
     2281 ccatatacag cctgttgaat acctcagagt acaagcgatt acagcctttc attgataacc
     2341 ctgagcctgg acctgatttt tttgatattt atcaatctgc aaaggatacc ttcccatcta
     2401 tcctgaatac tgacaaaccc aacatcgtgt tctttattct ggagagttgg agtgcggatg
     2461 tatgcaatca ggaaggcagg gacagtctga tagcgccctt cttcaatggt ctgatccgtc
     2521 agggattgta cttttcgaat tgctatgcaa gcggccatgt gtctgaccag ggtgtgcctg
     2581 cagctctggg cgccctgcct gtcataccct ccttttcctt gctgatgagc aagcaacaag
     2641 tgggtgagct gccatgtata accgatgact tgaaggctga tggttattat acaggatttc
     2701 tgtatggcgg tcagctggat tatggcaata ttcgcggcta tgtttttaaa aagggttttg
     2761 acgaagtagc agatgtccag tattttccat cttccacgcc aaagacagcg ttgggcgttc
     2821 ccgacctgcc cttatatatg gaattgttga gcaagctcaa cagcgcaaga cagccattct
     2881 tctattgcgg atacaacatc agcacccata gtccctatga tgttcctgtg gattatggat
     2941 tgggtatcgg aggtgaaaat gccgccttca tcaataccat tcattacgga gacagtagtc
     3001 tgagtgtatt ttttaaagag gcggaaaagc agccctggtt tgacaataca ttatttgtct
     3061 ttgtcgctga ccacagccat gagtcgcaga ttataagggt gcgtgaggat aaggatcgct
     3121 accgaatccc tttgctgctg tacggtccgg tattgagacc tgagttttgt ggtatggata
     3181 tacagaaagt agtttctcag ctagatctta ccggactact gctcagtcaa ttgggattgg
     3241 aatatagggg gaagtacccg ttcacccgaa atccgcttca atctgatttt aaggaaatgg
     3301 ccttctacaa tttcccgggc ggaagcggta ttgtcacacc caatagctgg ttgacaagaa
     3361 acctgaatga ccctaaagag cgggaacgta ttggcagtga taccctcaag gctgctttat
     3421 atggcgatat cggcaggtat ctccataaag cttccatata tctgaaggat agatagaata
     3481 ttttgaccct ttagcgcttt tccgctaatt tgttgaataa ttatcatatt tttgcaatgg
     3541 aatgtttgaa attatagtgt aaaatgtaca ctaagcataa aaatatataa tggacaaaat
     3601 aattacctta gatgaattta tcatccacca tcagttgcaa gttgatcccg gtgcaggtga
     3661 attgaccggt gtattgcggg atattggtgt ggcagccaag attatcaacc gcgaagtgaa
     3721 taaagccggt ctggtcaata tcctcggcgt cgcaggtaac cagaacgaat ccggtgatct
     3781 ggtacagaag ctcgatatct atgccaatga aaaaatcatc gattgtttga aaaacagtgg
     3841 tgactgctgt ggaattgcat ctgaagaaaa tgaaaacttt attcccttta aaggtgtagc
     3901 aggcaaagat tcaaaattta ttgttttatt tgatccatta gacggctcat ccaatattga
     3961 tgtcaatgtg tccgtaggca cgattttcag tatttacaaa cgattgagtc cgatggaagg
     4021 tgagtgtacg ctggaagact tccttcaaaa agggactcag caggtggcgg caggatatgt
     4081 actttacggt acatcaacca tcctcgtata taccacaggc aaaggagtca atggttttac
     4141 actcgatccg accatcggcg aattctgcct ttcccaccgg gacattaaga ttcctgaaaa
     4201 tggtgctttt tattcagtca atcaaggata ttatctgaag tttgatctgg agatgcgccg
     4261 ctatattgac tattgttctg acaataacct tggcttgcgt tatatcggtt ctatggtctc
     4321 tgatgtacac cggattttgt tccagggtgg catcttcctt tatcccaata cacgcaaata
     4381 ccctaaaggc aagttgagga tgcagtatga atgtaacccg atggctttta ttgttgaaca
     4441 gtgtgggggc aaagcgatga atacttcctt gcagcggatt atggagttgg atgctcatga
     4501 attgcaccaa cgatctacca ttgttttagg gtctccattg atggtggatg agatgaagcg
     4561 gtttgtagag aaatacagcc cgatgattta atcccggtaa atgccggtat tcattttaag
     4621 aattgcgtat agtctccttg ttcccgcttg ttgatttttg tacctgcatg atagccggtt
     4681 aaccaaatag atcaaatggt cagttaggtt gttcctgact aattgcgttc cgattgcccg
     4741 attgactatg tattgggtgg tagtgttcgt ttatactgaa taacaggagt tccggataag
     4801 actcttctca atccttacct tcatttagtt ttaagtacaa tgagtaatag tatcccttgg
     4861 ctttgagtag ttcgtcgtgt gtgccatcct cgatgacctt tccttctgcc attgtgatga
     4921 tcctgtcata attaaaaata ttattaatcc gctgggttat tatgatcatg gtgatgctgt
     4981 tgtcaacatc attaagataa gaggcgattt tcaattctgt atttgtatcc acagcggaaa
     5041 ggctattgtc cagaatcagt agttccggat tggatatcag cgatctggcg atggaaatcc
     5101 gttgtttctg gccaccggat aaggttacgc ctctttcgcc tactatcgtc tcatattcct
     5161 gcggtaaggt catgatatct tcatggacag aagcatgtac ggcgtatctc tccacgtcat
     5221 cgaatcccga ttcgtccttt aggccaaagc cgatgttgtt tcggatggag tccgagaaca
     5281 ggaatgaatc ctgtggtaca tagccgattc tttcccgcca tgattcaatg gaaaattcgg
     5341 agatatctct gtccccaatc aggatgcgac cttctgtcgg cttgtacatc tgcatcaata
     5401 ggtaggcaat cgtgctcttg ccggaagctg tttcgcctat gatggctgtt ttgctgcctt
     5461 ttttaataat aaatgatata tcattgaccg ccgttattcc cgtatcagga taagttaaag
     5521 acacatgctc aaagcgaata tcctggttga ttctttctat ttgatccgtt ccgtttttaa
     5581 aacttacatc agcatctaaa aattcgttga ttctcttttg ggaagctgct gcttgttgca
     5641 cttgtgatgc tgtccaccct aatgcggtga tcggccatgt caataaagtg atgtatatga
     5701 agaactccgc tatatttccg ggcgttactt cgccgcgcac cacctgatgt ccacctatcc
     5761 acaaagttaa cacggaagaa ataccgatca caaggatcat taacg
//
LOCUS       DAOQMC010000151         5757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6108_length_5757_cov_4.268327, whole genome shotgun sequence.
ACCESSION   DAOQMC010000151 DAOQMC010000000
VERSION     DAOQMC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6108_length_5757_cov_4.268327"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1220)
                     /gene="fbaA"
                     /locus_tag="PKV31_06940"
     CDS             complement(153..1220)
                     /gene="fbaA"
                     /locus_tag="PKV31_06940"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNB92629.1"
                     /translation="MSTRFRPGVLHGDEVTDALNYAKENEFAYPAVNVIGSSSINAVL
                     ETAKAVNSPVIIQFSNGGAAFNAGKGLKNENERSAILGAIAGSAHINTLAEAYGVPVI
                     LHTDHCAKKLLPWIDGMVEASERHFEKHGTALYSSHMIDLSEEPIHENISTCVAYLKR
                     MAAIGITLEIELGVTGGEEDGVDNSDVDSSRLYTQPEEVAYAFEELRKVSDRFTIAAA
                     FGNVHGVYKPGNVTLKPIILHNSQEYVQQKFNTGPKPINFVFHGGSGSSREEIREALS
                     YGAVKMNIDTDMQWAYWEGVKNYYKTNEGYLQGQIGNPEGDDKPNKKFYDPRVWLRAG
                     ENNFVARLKEAFDDLNCMNRN"
     gene            complement(1284..2225)
                     /gene="trxB"
                     /locus_tag="PKV31_06945"
     CDS             complement(1284..2225)
                     /gene="trxB"
                     /locus_tag="PKV31_06945"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNB92630.1"
                     /translation="MEQSKVLKCLIIGSGPAGYTAAIYAARAGLNPVLYTGNKPGGQL
                     TITTDVENFPGFPDGVQGPELVELFRQQAARFDTELHYENITKVDFSGPIHKAWDESG
                     KEILAHTVIISTGADAKWLGAPGEERLMNRGVSACAVCDGFFFRGMEVAVVGGGDSAA
                     EEATYLAKLCPKVHLIVRKDFLRASKIMAKRVMDNPKIQVHFNTETVEILGEDEVAGV
                     KLYNNATKEEFELPVKGFFIAIGHKPNTDIFSGYIELKEGGYIKTIQGTVKTNVEGVF
                     AAGDVQDDVYRQAITSAGTGCMAALEAEKYLINKGIE"
     gene            complement(2798..3340)
                     /locus_tag="PKV31_06950"
     CDS             complement(2798..3340)
                     /locus_tag="PKV31_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNB92631.1"
                     /translation="MTKIYYIIGMMGVGKSSIGLGAARHIHIPFVDLDDYIRKKENSS
                     ITEIFRTRGEAGFRALESKYLRELDFGDAHYAIVCTGGGTPTHHDNMAFMNKTGTTIF
                     LKTPIKEIIERLKGMDDDRPLLQTMESDNMAAQLTKIYESRLAFYEKAKYTLENIGYD
                     QDVIENLMELIRKDMETGEK"
     gene            3714..5447
                     /locus_tag="PKV31_06955"
     CDS             3714..5447
                     /locus_tag="PKV31_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNB92632.1"
                     /translation="MNGIIDQKVFAFRSKLEELAKDVHHLTVMIGHEELAETISNLRN
                     RINEPFMFVIVGEVKAGKSSFINALLDVGKEVAMVAPQPMTDTIQQIVYGEKEEIITI
                     NPFLKKITQPVDILKEIAIVDTPGTNTIVEKHQEITEGFIPGSDLIVFVFESKNPYRQ
                     SAWDFLNFIHSDWQKKVIFILQQKDLMEPADLLINMEGVRKQAESKGMNQPIVFAVSA
                     KMELDGNKEVSGFREVREYIAANITGGRAPYLKLENVLTTLVNIQHRIGHGLELRREQ
                     FVADTAFRKDITESLDQQEVRSGKHVEMLIENILNAYDNATGKAVKELDHGLSFPVML
                     KRSFSSIFSKKSTLKVWLQGVAGQLEDDLNNELRKKLNDGAGDVAESIHQMAKMIQLK
                     VQNSKTILKDDQDIFSDISERRSYIMTDLQNTFNSFLQNSDNFKDQTLFPDQSGVSAN
                     IATGSGIAVIGLIIAGVTHGAVFDVTGGLLTAVGLLFAGVTTMVKKNKIMSNFNQEIK
                     KGKEKLESEISTQLIQYISHLRHRIEDNFNRFDLMLAQEEKQIEAIDGAYKEVKGKLE
                     QSGSELHRMLN"
     gene            complement(5466..5609)
                     /locus_tag="PKV31_06960"
     CDS             complement(5466..5609)
                     /locus_tag="PKV31_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92633.1"
                     /translation="MIIDGTLSLKERLVLKNLSKEDWFPLTVSEVDDIVDGYIQGQGV
                     RKF"
BASE COUNT         1612 a         1168 c         1206 g         1771 t
ORIGIN      
        1 tgcttaaaat aaaaaaaaag gacgaaattt tatcgtcctt ttatcattcg aaaattatac
       61 tctgttagtt tttcccctgt atatcaattg gtagagttta gggaagtccg gatgcaggcc
      121 tgaaagccag gatccggcct gttttagatt attcaatttc tgttcataca gttcaggtcg
      181 tcaaatgctt ccttgagtct tgcaacgaaa ttgttttctc cggctctcaa ccatacgcgg
      241 ggatcgtaga atttcttgtt aggcttgtca tcgccttcgg gattgccaat ttgtccctgg
      301 agatatcctt cattggtctt gtagtaattc tttacaccct cccaataagc ccattgcata
      361 tccgtatcga tattcatctt gactgcgcca taggataatg cttcccgaat ttcttctcta
      421 ctggatccac ttcccccgtg gaagacgaag ttgatgggtt tcggtcctgt attgaatttt
      481 tgttgtacat attcctgaga attgtgcagg atgattggtt tcaatgttac atttcccggc
      541 ttatagacac catgtacatt gccaaaggct gctgcaattg taaaacggtc gctcaccttt
      601 ctaagttctt caaaggcata ggcaacttct tcaggttggg tatataacct actgctatct
      661 acatcggaat tatccacgcc atcttcctcg ccaccggtta caccaagttc aatctccagc
      721 gtaatgccga tggctgccat tcgtttcaga taagctacgc aggtacttat attctcatgg
      781 attggttctt ctgacagatc gatcatgtga gaactgtata gtgccgtgcc gtgtttttca
      841 aagtgccttt cacttgcctc gaccattccg tcgatccagg gtaggagttt cttggcacaa
      901 tggtcggtat gtagaatgac agggactccg tatgcttcag cgagcgtatt gatatgtgcg
      961 gagcctgcaa tagcgccgag aatggcagat cgctcattct catttttaag gcctttcccg
     1021 gcattaaaag cagcaccgcc gttagaaaac tggattatca ccggagaatt gaccgccttg
     1081 gctgtttcaa gaacagcgtt gatcgagctt gatccgatga cattgaccgc cggataggca
     1141 aattcgtttt cctttgcata gttcaaagcg tctgtaactt cgtcaccatg aagtacaccg
     1201 ggtctgaatc tggtggacat aagcaagttt attttttgta aattttagca atcggttatt
     1261 tcaaagacca aagctaagta aaattattcg attcctttat tgatcagata tttttcagct
     1321 tcaagagcgg ccatacagcc tgttcctgcc gatgtgatag cctgacgata gacatcatcc
     1381 tgcacatctc ctgctgcaaa aacaccttcc acattggttt tgacagttcc ctggatggtt
     1441 ttgatataac cgccctcctt caattcgatg tagccgctga aaatatccgt attgggtttg
     1501 tgcccgatag cgatgaagaa tcccttgaca ggcaactcaa attcttcttt ggttgcgttg
     1561 ttgtataatt ttacacctgc cacttcatct tctcccagga tttctaccgt ttcggtattg
     1621 aagtgaacct gaatctttgg attatccata acgcgctttg ccataatctt ggaagcccta
     1681 agaaaatctt tcctaacaat aagatgcacc tttgggcaca acttggcaag ataggtagct
     1741 tcttcagctg cagaatcgcc acctcccaca acggccacct ccatgccacg gaagaaaaag
     1801 ccatcacata cagcacatgc agacaccccc ctgttcatca gccgctcctc tccgggagcc
     1861 ccaagccatt ttgcgtcggc tccggtggaa atgataaccg tatgggccag tatttctttt
     1921 ccactttcat cccaagcctt gtggatagga cctgaaaaat ccactttggt tatattttca
     1981 taatgtaatt cggtatcgaa tctggcagcc tgctgtctga acagttctac taattcaggt
     2041 ccctgtactc catccgggaa tccgggaaaa ttctctacat cagtagtaat tgtcaattga
     2101 cctcccggtt tgttaccggt gtacaaaacc ggatttaaac cggctcttgc tgcatagata
     2161 gctgctgtgt agcctgccgg tccgctgcct ataatcaggc actttaatac ttttgattgt
     2221 tccattgtta attattttcc agataataca atgtaatttt tgcataaaag tttgaaaaaa
     2281 agaaaactta atttggttga agagttgtgg atataataaa tgccatacaa attatcgagt
     2341 taatagcaac tccatatctt ggttgaggtg aataattgat tgtgcttttt gtcggtacag
     2401 acttactctt gattggtggg tataccggaa agtaataata aacattttat aaaaataaga
     2461 taatcaacgt tgtatacatg gcgcatttgt tatgaaaaaa tggtgtatta agtgcaaagc
     2521 tgtttacgat tctaaactaa gtcttagtga ggtaaactgt acaaaagcat aacaattgtt
     2581 tttatgcctg ccggttgagg attaatcatt tattgaaatc tttgtctgta agctttgtag
     2641 tttgcggctt agtggacatt ttttaggctc aaaatcgcca taagtcttgt tgtacctatc
     2701 tttgaagtca atcaaaggtt atgaatatat aaagtgctat gcatgggcga tcctttatag
     2761 taattatacc aaactaaatc ttgttatttg gcagtgctca tttctcaccc gtttccatat
     2821 ccttcctgat caattccatg aggttttcaa tcacatcctg atcatagccg atattttcaa
     2881 gtgtatactt tgccttttca tagaaagcaa ggcggctttc ataaattttt gttagctgag
     2941 cagccatatt gtcactttcc atggtttgaa gcaaaggtcg gtcatcatcc atacctttca
     3001 gcctttcaat gatctccttt atcggggttt tgagaaagat ggtcgttcct gttttgttca
     3061 tgaaagccat gttgtcatga tgagtcggag tgcctccacc ggtacagact atggcataat
     3121 gtgcatcgcc aaagtcaagt tcacggagat attttgattc caaagctctg aaaccggctt
     3181 ctcctcgcgt tctgaatatt tcagtaatgg atgaattttc ttttttgcgg atgtaatcat
     3241 ccaaatcgac aaatggaata tggatatgtc tggcagctcc taaaccaatc gatgacttac
     3301 caacgcccat cataccgata atgtaataaa tttttgtcat aatgcaggta gatgtattaa
     3361 aaatgtgtta gttattctgc aaattaaaca ataattatta aaaaaaatcg aatgttcccc
     3421 aatttatttt ggtctaaatc gaccaatcta cgttttaatt gaatattgaa gttattgtaa
     3481 ttttgtctaa tttgttgtaa cccattctga tatgcctctt atttgacctg atttcaatgc
     3541 ttattttgag aagttaaatt tttaggttgg aatcaggatg tccggtaagt ttaactatta
     3601 atacatttta ttattatttt tgaaagggaa tgtgattttc ttagtataac aaccgttctg
     3661 tggtttttga tagtaatttg cctgtatttc tgaaatctgt ttaatttagc atcatgaatg
     3721 gaattatcga ccaaaaggtt ttcgctttta gatcaaagct tgaagaactt gccaaggatg
     3781 ttcatcatct gacagtgatg atcgggcacg aggaactggc agaaaccata agcaaccttc
     3841 gcaacagaat aaatgaaccc ttcatgttcg ttattgtcgg cgaagtgaaa gcagggaaga
     3901 gtagttttat caatgccttg cttgatgtag gaaaggaagt ggccatggtc gctccacagc
     3961 ccatgacaga tacgattcag cagatagtat atggagaaaa ggaggaaata atcacaatta
     4021 atcctttttt gaaaaaaatt actcagccgg tcgatatatt gaaagaaatt gcaattgttg
     4081 atactcccgg aaccaacacc attgtggaga agcatcagga aattaccgag ggatttattc
     4141 cgggcagtga tctcatcgtg tttgtatttg aaagtaagaa cccctatcgt cagagtgcat
     4201 gggattttct gaatttcatc cattcagatt ggcaaaagaa ggtaatattt attttacagc
     4261 aaaaagattt gatggagcct gcagatcttc ttattaatat ggagggagtc aggaaacagg
     4321 ctgagagcaa agggatgaac caaccgattg tatttgctgt atcagctaaa atggaattag
     4381 atggtaataa ggaggtaagt ggatttcggg aagtgcggga gtatattgcc gctaatatta
     4441 ccggaggtag agccccttat ctcaagctgg aaaatgtcct gaccacctta gtaaatattc
     4501 aacataggat cggccatggc ctggaattgc gaagggaaca gtttgtagct gatactgcct
     4561 tccgaaaaga tattacagaa tcgcttgacc aacaggaggt cagaagtggt aagcatgtgg
     4621 agatgctcat cgagaatatc ctcaatgctt atgataatgc gacgggaaag gcagtcaagg
     4681 aacttgatca cgggctttcc ttcccggtaa tgttgaagcg atccttttct tccatttttt
     4741 cgaagaaatc cacccttaaa gtctggttgc agggtgttgc cgggcaactt gaggatgatc
     4801 tcaataatga gttgcgcaaa aagctgaatg atggggcagg agatgtggct gagagcatcc
     4861 atcaaatggc taaaatgatt cagttgaagg tgcagaacag taaaaccatc ctgaaagatg
     4921 atcaggatat tttttcagat atttcagaaa gacgaagcta tattatgacg gatcttcaga
     4981 ataccttcaa tagctttctt caaaattcag ataatttcaa ggatcagacc ttattcccgg
     5041 atcaaagcgg cgtttctgcc aatatcgcaa ccggtagtgg tatcgctgtg ataggattga
     5101 ttatcgcagg ggtgactcat ggcgccgtat ttgatgtcac cggaggcctt ctgactgctg
     5161 taggtctgtt gtttgcgggc gtaacgacga tggtcaagaa aaataagatc atgtccaatt
     5221 ttaaccagga gatcaaaaag ggaaaggaaa aactggaatc tgaaatcagc actcaattga
     5281 tacagtatat ttcacatttg aggcaccgta ttgaagacaa ctttaaccga tttgatctta
     5341 tgttggctca ggaagaaaaa cagatcgaag ctatcgatgg agcctataaa gaagttaaag
     5401 gcaagcttga acaatccgga agtgaattgc accgtatgct taattgagtt gcatggccaa
     5461 caagctcaaa actttctgac cccttgtcct tgaatataac catcgactat gtcgtccacc
     5521 tcgctgactg taagcgggaa ccagtcttcc ttactcaggt ttttcaatac aagacgttct
     5581 ttcaaggata gagtcccatc tatgatcata ccgaatatca tcaaatactg ggacctccag
     5641 ccgatttcga agttcgcgcg gcacgcggcg gagttcgtga acctgtactc aaagtggcgc
     5701 ggcatcaatc gtttcccggc cttgacccgt atgttcgatc tccttagcga ttgggac
//
LOCUS       DAOQMC010000152         5756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6110_length_5756_cov_4.977548, whole genome shotgun sequence.
ACCESSION   DAOQMC010000152 DAOQMC010000000
VERSION     DAOQMC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6110_length_5756_cov_4.977548"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PKV31_06965"
     CDS             <1..804
                     /locus_tag="PKV31_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNB92634.1"
                     /translation="KKYNMDTDLASQMEDAMECAMDLSRSGKGDMIKLVYEKNIIEGE
                     EVDAGKLLAAYYKGRLGEKYVFYFDNGKYKGYFDQDGRPMKKSYLASPLKYAKITSKF
                     SMSRLHPILNKVVPHFGTDYAAPTGTPILAVSDGVIEAQGYGGGNGNYVKIKHDRTYS
                     TQYLHMSRFPKGQRKGQRVRQGQVIGYVGSTGLATGPHVCFRFWKNGRQVNPKSCKLP
                     APNPIPGSSLTEFKALRNDMLSKLAAIGKPLPGETLVKKSEDELFLIEP"
     gene            complement(817..2694)
                     /locus_tag="PKV31_06970"
     CDS             complement(817..2694)
                     /locus_tag="PKV31_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNB92635.1"
                     /translation="MISISNLYIQYGDRILFDSISTTINIGEKIGLTGRNGAGKSTLL
                     KILAGEITQFDGVVDRPRNETVAYLHQDIEISGEHSVIEEAMKAFEELNEIERQIQIA
                     EHDLTIRDDYESDSYAKLIDNLTHLTEQFEYRGGNQKEANAEKILKGLGFKSHEFSKK
                     VKELSGGWQMRVELAKMLLTKPDYLLLDEPTNHLDIESIIWLESFLSDYKGAVVMISH
                     DKIFLDKITRRTIEIELGRIYDYQANYSDYLLQRVERRALMMAAYENQQKLIAQKEKT
                     IARFMAKATKTSMAQSMQKQLDKIERIELEDEDTSAIRLSFPSAPRSAEVLLDASGLE
                     KSYNGVNVFRDVDFRITRGERVAFVGQNGMGKSTLAKILIGKEKADKGTVVIGPNTFL
                     GYYAQNQTEVLQGNLTLLETMENESPPERRTQLRNILGSLMFSGEDVDKKVSVLSGGE
                     RGRLALALLLLHPINLLVLDEPTNHLDLKAKEVLKKAIMDYDGTMIVVSHDRDFLSGL
                     TEKTIEFRDNTIKEYLGDVNYFLDKRQMETFREVEAVQKQTANSQPKNSARVDNSKKQ
                     EIEKTEKRINELEAKIKSLHDKMMTDDFYNSPDYQKTTTQYANLQKELEIEMTRWEEM
                     L"
     gene            3009..3692
                     /gene="rpiA"
                     /locus_tag="PKV31_06975"
     CDS             3009..3692
                     /gene="rpiA"
                     /locus_tag="PKV31_06975"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNB92636.1"
                     /translation="MLTQDQIKEKIGIHAADYIKPGMKVGLGTGSTVFYLLKELGRRK
                     AQGLHFTAVCTSVQTQRVLIEEGIEFLTLDEVDRLQIAIDGADEIDPAGNMIKGGGGA
                     LLREKIVEYNADELIIIIDEKKRVNNLGAFPLPVEVVTFGWKQVRQKLANDYGIIVLR
                     REHDSKPFITDQGNYILDCHFSLITDPQQLNTDIQLIPGVVETGLFVGMADKAITGYA
                     DGSIEFIEF"
     gene            3865..4725
                     /locus_tag="PKV31_06980"
     CDS             3865..4725
                     /locus_tag="PKV31_06980"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNB92637.1"
                     /translation="MRIISGLFLFAACFLSGYVLNAQIKTPAASPGSTVKANVGLGEA
                     TVEYSRPSKKGRIIFGDLVPFGKIWRTGANGATKITFSEKATVGSSELAKGTYAIYTI
                     PNPNEWTVIFYKDLTKGGGLTDANIKSEDVAAKIVVQTAMINPGVETFTIDIADVTNK
                     GASIAIMWDKTMVKVPFTYNTDAAVMANIKEKMAGPSGNDYYAAAQYYYNEDKDLNEA
                     LVWIREATKMNSDRFWIWRQQSLIEAKMGKYADAIKSAEKSLALAKEAGSDDYVKMNN
                     ESIKEWSAKK"
     gene            4822..5166
                     /locus_tag="PKV31_06985"
     CDS             4822..5166
                     /locus_tag="PKV31_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB92638.1"
                     /translation="MKYFILAATLSLFMASCSNDNPNNKSTENHLSSLTVDERMAEAK
                     ELFEGQCKLCHGIDGTLGLNGAKDLSKSTLSKEERIDIITHGKNAMAAYKDEYSPQEI
                     VMLAEYVETLKK"
     gene            5170..>5756
                     /locus_tag="PKV31_06990"
     CDS             5170..>5756
                     /locus_tag="PKV31_06990"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNB92639.1"
                     /translation="MNVYHGLGVMSGSSLDGIDFALCRFTFDETADNPILEWHIVEAE
                     TFELSVFWEERLKKAYLASAKELWLAHVTFGKYIGDLANTFMKRLDIKPNFISSHGHT
                     VFHEPQNHMTLQLGHGAAIAGATGYPVICDFRSTDVALNGQGAPMIPIAENLLFSEYP
                     LCLNIGGIANISARVDSHYIAFDVSPANQVLNKLAS"
BASE COUNT         1711 a         1210 c         1183 g         1652 t
ORIGIN      
        1 aagaaataca atatggatac ggatctggcc agtcagatgg aagatgcgat ggaatgtgcc
       61 atggacctca gccgttcagg taaaggtgat atgatcaaac ttgtttatga aaaaaatatc
      121 atcgaaggag aggaagtaga tgccggcaaa ctactcgctg catactacaa aggcagattg
      181 ggcgaaaaat acgtgtttta ctttgataac ggtaaatata aaggatattt cgatcaggat
      241 ggaagaccta tgaagaaatc atatctggca tctccactga aatatgctaa aataacctca
      301 aagttctcca tgtccagact tcatcccatt ctcaacaaag tggtccctca ttttggaaca
      361 gactatgcag ctccgaccgg aactcccatt ctcgccgtat ctgatggcgt gatcgaagct
      421 cagggctacg gtggcggtaa tggtaattat gtaaaaataa agcacgatcg tacttattca
      481 acacagtacc tgcatatgtc acgcttccct aagggacaga gaaaaggtca aagggtaaga
      541 caaggacagg taattgggta tgttggttca accggtctgg ctaccggacc tcatgtgtgt
      601 ttcaggttct ggaagaatgg aagacaggtt aatccaaaaa gctgcaaact acctgctcct
      661 aatccgattc cgggctcaag tctgacagaa tttaaggctc tacgaaatga catgctgagt
      721 aaattagcag ccatcggaaa accacttccg ggagaaacat tggtcaagaa aagtgaagat
      781 gaattattcc tgattgaacc ttaatcaggg acaatatcat agcatttctt cccaacgcgt
      841 catttctatt tccagctctt tttgtaggtt ggcgtattgc gtggtcgtct tttgataatc
      901 aggtgaattg tagaaatcgt cagtcatcat tttatcatga agcgatttga tttttgcctc
      961 aagttcatta atgcgctttt cggttttctc aatctcctgc tttttactat tgtcaacacg
     1021 ggcagaattc tttggttgtg aattagcagt ttgcttttgc actgcttcca cttcgcggaa
     1081 tgtttccatc tgccgtttgt ctaagaaata gttgacatcc cccagatatt ctttaatcgt
     1141 attatccctg aattctatgg tcttttcggt caacccggaa agaaaatccc ggtcgtggga
     1201 gactacaatc atcgtgccat cataatccat aatggctttt ttaagcactt ccttagcctt
     1261 caggtcaagg tgattggtag gttcgtcgag cacaagcaaa ttgatggggt gtaaaaggag
     1321 taaggccaat gccaacctgc cacgctctcc tcccgacaaa acgctcacct ttttgtccac
     1381 atcttcaccg gaaaacatga gggaacctaa aatattccga agttgagttc gtctctccgg
     1441 tggcgattca ttttccatag tttcaagcaa ggttaggttg ccttgcagga cttcagtttg
     1501 attctgcgcg taatatccga ggaaggtgtt cggcccgatt accacggttc ccttatctgc
     1561 cttttctttt ccaattaata ttttggccaa ggtagatttc cccattccat tctgtccaac
     1621 gaatgctacc cgctcacctc ttgtaatacg gaagtccaca tcccgaaata cattgacacc
     1681 attgtatgac ttttccaatc cggaagcatc cagcaaaact tcagcagaac ggggagctga
     1741 gggaaaactc aaccggatag ccgatgtatc ttcatcttcc agttctatcc gttctatttt
     1801 atccagttgt ttttgcatgg attgtgccat ggaagtttta gtggctttgg ccataaaccg
     1861 ggcaatggtc ttttctttct gggcaatcag cttttgctga ttttcatagg cagccatcat
     1921 gagagccctg cgctccaccc tttgtaacag ataatcagaa taattggcct gataatcata
     1981 gattcgaccc aattcaattt caatggtacg cctggtaatt ttatccaaaa agatcttatc
     2041 gtgtgagatc atcactacgg cacccttata gtcagacagg aaagattcga gccaaataat
     2101 tgattcaata tccaggtggt tggtaggttc gtccagtaac agataatccg gcttagtaag
     2161 caacatcttg gccagctcga ccctcatctg ccatccaccg gacaattctt tgactttctt
     2221 ggaaaattca tgacttttaa aaccaagccc tttcaatatt ttttctgcat tagcttcctt
     2281 ttgatttcct cccctgtatt caaattgttc tgtaagatgt gttagattat cgatgagttt
     2341 agcataggag tctgactcat aatcgtcgcg tatagtcaga tcgtgctcgg caatttgtat
     2401 ctgtcgttca atctcattca actcctcaaa ggccttcata gcttcttcta ttacagagtg
     2461 ttcgccgctg atctcaatat cctggtgcag atacgcaaca gtctcgttac ggggacggtc
     2521 taccaccccg tcgaactggg taatttcacc cgccaatatt ttgagcagcg ttgatttgcc
     2581 tgcaccattt cttcctgtaa ggcctatttt ctctccgata ttgatcgtag tcgatataga
     2641 atcaaaaagg attctgtcac cgtattgaat ataaagattg ctgattgaaa tcattataga
     2701 aatcgaatgt tcactaaatt aaggctgcaa agtaaagcaa aactttccta atcatcaatg
     2761 cataactaca aattgacaaa cgtatgtccg acgatgatat aatttgttca gaatcagacc
     2821 aaatacacta cactatacaa atggtatgca gccctgttgg aaatggaaaa aaattttaaa
     2881 ataaacactc cggagcaaaa cttaaaaata tacttctttt tgcagccttt catttaccaa
     2941 cctattttcg tcaatattct aacccttaaa tcattctgct ttaccaaaat tacccttaca
     3001 tttacaccat gcttacacaa gatcaaatca aggaaaaaat aggaatacac gccgctgatt
     3061 atatcaaacc gggtatgaag gtcggattgg gtacaggctc gactgttttt tacctattga
     3121 aggaactggg gcgaagaaag gcgcaaggcc tccatttcac tgccgtatgc acttccgtac
     3181 aaacgcagcg tgtactcatt gaagaaggaa tcgaattcct cactctggat gaagtggata
     3241 gattacaaat tgcaattgat ggtgctgatg aaattgatcc tgccggcaat atgatcaagg
     3301 gaggtggagg cgcattactt cgggagaaaa tcgtagaata caatgctgat gaactgatca
     3361 taatcattga tgaaaaaaaa cgagttaaca acctcggtgc atttccacta ccggtcgaag
     3421 tggtcacttt tggctggaag caagttcgtc aaaaattagc aaacgactat ggtattattg
     3481 tacttagacg tgagcatgat agcaagccct tcatcactga ccaggggaat tatatcctgg
     3541 attgtcattt cagcttgatt actgatccac aacaactcaa taccgacatc caattgatac
     3601 ctggagttgt cgaaacgggt ctttttgtgg gtatggcaga caaagcaatt acaggatatg
     3661 cagatggatc cattgaattc attgaatttt aacgggatcg gaggaggaac tccatttatt
     3721 tatctttgga cttatttgac cctaatattt tctttatcaa taagttctat acttttctct
     3781 caaacgattg aaccttttca ttcaatctgt gttaaccttc gaataaacct ttgaattgtt
     3841 ttttatcaac aaaaccatat aataatgaga ataatttcag gactttttct ttttgcagcc
     3901 tgctttcttt caggctatgt tctaaatgca caaatcaaga cccctgcagc ttctccgggc
     3961 tctaccgtta aagctaatgt aggtttgggt gaagcaaccg tagaatattc aagaccttcc
     4021 aagaaaggca ggattatttt tggcgacctt gtaccattcg gtaaaatatg gcgtaccggt
     4081 gccaatggtg cgacaaaaat caccttttct gaaaaggcaa cagttggctc cagtgaattg
     4141 gcaaaaggaa catacgctat ctacacgatt cccaatccga atgaatggac tgtaatcttc
     4201 tataaagatc ttacaaaagg aggtggactt acagatgcca acatcaaatc agaggatgtg
     4261 gccgctaaaa tcgtggtgca aacagccatg ataaaccccg gtgtagagac atttacaatt
     4321 gatattgccg atgttaccaa caaaggtgcc agtatcgcca tcatgtggga taagactatg
     4381 gtgaaagttc cgtttaccta caacacagat gcagcagtca tggccaacat taaggagaag
     4441 atggccggtc cttccggtaa tgactattat gcagcagctc aatattatta caatgaagat
     4501 aaggatttga atgaagctct tgtatggatt cgggaagcta ccaaaatgaa tagcgacagg
     4561 ttttggatct ggagacagca gtcgttgatc gaagctaaga tgggcaaata tgccgatgcc
     4621 atcaaatctg ccgaaaaatc tcttgcactt gcaaaagaag ccggtagtga tgattatgta
     4681 aaaatgaaca atgaatccat caaggaatgg agtgcaaaaa aataatttct ttgaaagaag
     4741 caagattgga aagcgcgtcg aaagacgcgt tttttatttg ttgaagtttc attacttttg
     4801 gcgacacatt tcacaaaatt catgaaatat ttcatacttg cagcaacttt atcacttttc
     4861 atggcatcgt gctccaatga caacccaaat aacaaatcca ctgaaaacca tttatcctcc
     4921 ttgacagtgg acgagagaat ggcagaagca aaggaattgt tcgaagggca atgtaagtta
     4981 tgtcacggta tagatggcac cttgggcctt aatggcgcca aagacctcag caaatccact
     5041 ttgagcaaag aagaaagaat agatatcatt actcatggca agaatgccat ggctgcctat
     5101 aaagatgaat attctccgca ggagatagtt atgctggcag aatatgtaga aacattaaag
     5161 aaataaaaga tgaatgtata tcacggtctc ggcgtcatgt ccggttcttc tttggatggt
     5221 attgattttg cattgtgtcg atttaccttc gatgaaacag cagataaccc aatccttgaa
     5281 tggcatattg tagaagccga gaccttcgaa ctaagtgttt tctgggaaga aaggctaaaa
     5341 aaagcatatt tggcctcagc aaaagagctt tggctggcgc atgttacatt cggaaaatat
     5401 ataggcgatc tggccaatac tttcatgaaa aggctggata ttaagcctaa ttttatttcc
     5461 tcccacggac atacggtatt ccatgagcct caaaaccata tgacacttca actgggacac
     5521 ggagcggcta ttgccggtgc aacaggatat cctgtgattt gtgattttcg gagtacagat
     5581 gtcgcactca acggccaggg tgcacctatg attccaattg cggaaaatct gcttttctca
     5641 gagtacccgc tttgcctcaa tatcggcggc attgcaaata tcagcgcccg agtggatagt
     5701 cactacatag ctttcgatgt aagtccggcc aatcaggtat tgaataaact ggcttc
//
LOCUS       DAOQMC010000153         5754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6117_length_5754_cov_4.400948, whole genome shotgun sequence.
ACCESSION   DAOQMC010000153 DAOQMC010000000
VERSION     DAOQMC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6117_length_5754_cov_4.400948"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PKV31_06995"
     CDS             <1..956
                     /locus_tag="PKV31_06995"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HNB92640.1"
                     /translation="NTSENATSYTWSFGDGTTSTDKSPKKAYTAVGEYTVNLTATGEG
                     GTATASAKITVTKPVSKKTLYYIELGDKNKISKVSLTEGSQAELFLDIVGKGGVGLAY
                     DATNDKIYYSDFENADEGIIWRMNGDGTGLEKIASGITDPYSIAIDLKGGKIFWADDN
                     GNISRANLDGSNVEKSFINVADGQMRGIAFNSKTNKLYFYEVNNEDLYIANADGTGVA
                     VLIPGVYGYGIFVDEVNEKLYYDERNAQALMQASLDGTGAKTISSTGKTRIHGIAIDY
                     DANKLYWADRDKGEIKRANLDGSGEESFLSGLDSPRGIFIK"
     gene            1120..2253
                     /locus_tag="PKV31_07000"
     CDS             1120..2253
                     /locus_tag="PKV31_07000"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB92641.1"
                     /translation="MKSILKLLLIVIMLNGIVSCDHDFPVPPASIVPKFTYVADNDGT
                     APSTVTFTNTSIVPTEYGAPTYTWNFGDNTTSTETNPVHRYENPGVYQVSLVIVATGT
                     GQIKTITQAINIKNPDASGVPIYFTNSTQVLQAYINDDAPVFEVLPVTNIKDSYGICI
                     DTIHDVLYIADLDGGAIIQTDLNGKNQKIWRSGLDSPSGMAIDYEKNELYWDTSNGIQ
                     KGDLNSDDVNQKVDFITGQTNDPDGIAIDPVNRNLYWINYDGGLGMKGLDGSGEKILI
                     PDAEGARIIVVGNRIYFDYYNGSGDIHIKSANLDGSNLATLATGISRVVYGLAYDPYG
                     NKIYWGDRNKGQITRADLDGSNVQTFYTAAGSTPRGIVFGKKK"
     gene            2256..3098
                     /locus_tag="PKV31_07005"
     CDS             2256..3098
                     /locus_tag="PKV31_07005"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNB92642.1"
                     /translation="MYNKIIISFLTLFLCLDLTSQSISEEGKLFIIGGGRKPASMIQR
                     MVKEANFKQGDYAMILPMASGAPHSAINEMKKALAESGIMECYGINFDQSMIKDPIKL
                     DSVKNARLIFLSGGDQNRFMHLVLNSPLEQAIKDALSNGALIAGTSAGAAMMSKYMIT
                     GNQLQDTVYTATFKNIIAENVEIAMGLGLLVNAIVDQHFLIRSRHNRLLTAICEYPGN
                     TGIGIDESTALLVKGNIGTVVGESQVLVYRLDSRPKVGKNGKFSVPKIRTEIYTEGDS
                     FKIK"
     gene            3150..4406
                     /locus_tag="PKV31_07010"
     CDS             3150..4406
                     /locus_tag="PKV31_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB92643.1"
                     /translation="MADCQISGIERKIVEKVDRNHDEALAFLKKIVNINSGTMNFAGI
                     KKVGDEFAERYRKLGFDVQWIPGDSFGRAGHLVATRLAAKGPKILMIGHLDTVFELDS
                     PFQEYTMIDDHLMKGPGVLDMKGGNVVMYLAIKALYELKLLDNLSIKVFLVGDEELSG
                     EPIALSKKDLIEAGKWADIALGYEDGDGTFDHANISRRGSADWTLKVKGIPAHSSQIF
                     QPEVGAGAIYETSRILHEFYKQLSNEENFTFNPGLISGGNSLTFHEETNKSEAFGKNN
                     IVSAEVIVTGDMRAVSMEQLIRTRRIMQEIVSNSLPNTSATIEFGDDGYPPMTMTEGN
                     IQLLGLFSKVSQDLGYGTVHPVNPRNSGAADISFVAEDVFMAMDAIGLCGTGGHTIEE
                     TADISCLPKEAKRTAILLYRLKDIKF"
     gene            4412..4840
                     /locus_tag="PKV31_07015"
     CDS             4412..4840
                     /locus_tag="PKV31_07015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNB92644.1"
                     /translation="MEEWSETKANEIISELMLSNDAFSKWLGVRVDLVKESSVEISCN
                     ITETMTNGFHVAHGGIVFSLCDSALAFHSNSWGRKALSIETSINHLSPIRVGDRITAK
                     TSIIKVGKSIGNFLIEAYNQDDKKVAIMKGMVFYLGDRWA"
     gene            complement(4848..5195)
                     /locus_tag="PKV31_07020"
     CDS             complement(4848..5195)
                     /locus_tag="PKV31_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB92645.1"
                     /translation="MAIIITDECINCGACEPECPNNAIYEGGVEWRMKDGTTVVGDYT
                     LEDGSVVRYDQLMSPVSDDYYYIVPDKCTECVGFHEEPQCAAVCPVDCCVPDPDRRES
                     DEILLMRKGRLHG"
     gene            complement(5249..>5754)
                     /locus_tag="PKV31_07025"
     CDS             complement(5249..>5754)
                     /locus_tag="PKV31_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB92646.1"
                     /translation="PQRDNLEPFDLIILKDVIEHIPDQEKIIPELKKLSKPGGTLFFA
                     FPPWYMPFGGHQQILRSKILSKIPWIHLLPGPLYRSLLQMAGEAESTVKELMELKATG
                     ISIERFNAIARQHFNIIDTSFWLINPIYEYKFKLRTRKVPGFLTKLPFFRNFYTTAAY
                     FTLRNDK"
BASE COUNT         1817 a         1060 c         1242 g         1635 t
ORIGIN      
        1 ccaatacttc agaaaatgcg acttcatata cctggtcatt tggagatgga acgacttcaa
       61 ccgataaaag ccctaaaaag gcttacaccg ctgtcggcga atacacggtc aacttaaccg
      121 ctaccggaga agggggaaca gccactgcca gtgcgaaaat cactgtaacc aagccggtaa
      181 gcaagaaaac tctttattac atcgagctgg gggacaagaa taaaattagt aaggtttctc
      241 ttaccgaagg ttctcaggct gagttgtttt tggacatcgt cggcaaggga ggagttggat
      301 tagcatatga tgccactaat gataaaatct attacagcga cttcgaaaat gctgatgaag
      361 gtatcatatg gcgtatgaat ggagatggta caggattgga gaaaatagca tccggaatta
      421 ctgatcctta tagtattgcc attgatttga aaggaggaaa aatattctgg gcggacgata
      481 atggtaatat ctccagggca aatctggatg gaagcaatgt tgagaaatct ttcattaacg
      541 tagctgacgg acaaatgaga ggtattgcct tcaacagtaa gaccaataag ctgtattttt
      601 atgaggtaaa taatgaagat ctgtatatcg cgaatgcaga tggaaccggt gtagcagttt
      661 tgatacccgg cgtatatgga tatggaattt ttgttgatga agtaaatgaa aaattatact
      721 acgatgaaag aaatgcccaa gcgctaatgc aggctagtct ggatggcaca ggggcaaaga
      781 caatttcatc taccggcaaa actcgtatcc atggcatagc aattgactat gatgccaata
      841 agttgtattg ggcagacagg gataaaggtg aaataaaaag agctaatctc gatggaagtg
      901 gtgaagaatc cttcctcagt ggacttgaca gcccaagagg aatattcatc aagtaatcta
      961 atcatttgaa aaaagctcat tgataggaat atatagtgaa tcagcaatct atcaaagttt
     1021 gattgctgat tcactttaat aagtatttcc attaactaat ttcaacttgt tccgaatcag
     1081 gaagtgtttc ttattaattc atcttaaatt tcgtatggaa tgaaatctat acttaaatta
     1141 ttacttatcg taatcatgct taatggcata gtttcatgtg accatgattt tccggtacct
     1201 ccggcgagta tcgttccaaa atttacctat gtagctgaca atgatggtac agctccttcg
     1261 acggtaactt ttactaacac ttccattgtc ccgacagagt acggagctcc tacttacacc
     1321 tggaattttg gagataatac aacctctaca gaaacaaatc cggtccatag gtacgaaaat
     1381 cccggtgtct accaggtttc tttggttatt gttgcaacag gaacaggcca gataaagacg
     1441 attacacagg ccatcaacat taaaaatcca gatgcgtcag gtgttcctat ctattttacc
     1501 aatagcactc aagttcttca ggcatatatc aatgacgatg ctccggtttt tgaagttttg
     1561 ccagtaacca atataaagga ttcctatggt atttgtattg ataccatcca tgatgtactc
     1621 tatattgctg atttggatgg tggggcaata attcagactg acctcaatgg aaaaaatcaa
     1681 aagatttgga ggagtggtct tgattcgcca agcggcatgg caatagacta tgaaaaaaat
     1741 gaactttatt gggatacttc caatggtatt caaaaaggag acttaaatag tgatgatgtc
     1801 aaccaaaaag tcgatttcat caccggtcaa acaaatgatc ctgacggaat tgctatcgat
     1861 ccggtcaatc gtaatctgta ttggatcaac tatgatggtg gattaggcat gaaaggcctg
     1921 gacggaagtg gagaaaaaat cctgattcca gatgcagaag gggcaagaat cattgttgtt
     1981 ggcaacagaa tatattttga ttactataac ggttccggtg atatccatat caagtctgcc
     2041 aatttggatg gaagcaatct tgctacactt gctacaggaa tctcaagagt tgtatatgga
     2101 ttagcatatg atccctatgg gaataaaatt tattggggag acagaaataa aggtcaaatt
     2161 accagagctg accttgatgg gtcaaatgtt caaactttct acacagctgc cggaagcaca
     2221 ccgagaggta ttgtttttgg caaaaagaag taatcgtgta taataaaatt atcatatcat
     2281 ttctgactct ctttctctgt cttgatctga cttcacagtc aatttctgaa gaaggcaaat
     2341 tattcatcat tggtggtggt cgcaaaccgg cctcaatgat tcaaagaatg gtaaaggaag
     2401 ctaatttcaa acaaggagat tatgcaatga tacttcctat ggccagtgga gcgcctcatt
     2461 ctgcaatcaa tgaaatgaaa aaagctttgg cagagtcggg tattatggaa tgttatggga
     2521 tcaacttcga tcaatcaatg atcaaggatc caatcaaatt ggattctgta aaaaacgcaa
     2581 ggttaatctt tctgagtggt ggcgaccaga acagattcat gcatcttgta ctaaattcac
     2641 cattggaaca ggcaatcaaa gatgctttgt ctaacggtgc attgatagca ggaacaagtg
     2701 ccggggccgc aatgatgtcc aaatacatga tcaccggcaa tcaattgcag gatacagtat
     2761 acacagctac ctttaaaaat ataattgctg aaaatgttga aattgctatg ggcttgggat
     2821 tattggtaaa tgcgatagta gatcagcatt tcctgataag gagcagacat aatcgattat
     2881 tgaccgcaat ctgtgaatat cccggaaata ccggaatagg tattgatgag tctactgcat
     2941 tactggtcaa aggaaatatt ggaaccgtag tgggggagag tcaggtatta gtatatcggc
     3001 tggattcccg tcccaaagtt ggcaagaatg gaaaattttc cgtccccaaa atcaggactg
     3061 aaatatatac cgaaggagat agttttaaaa tcaagtaatt gatgatgagt agattttttc
     3121 tgatcacaat atgtatattt tgggctacaa tggctgattg tcaaatttcc ggaattgaaa
     3181 gaaaaatagt agaaaaagtt gaccgcaatc atgatgaagc cttggcattt ttaaaaaaaa
     3241 tcgtcaacat caatagcggt acaatgaatt ttgccggaat aaaaaaagta ggcgatgaat
     3301 ttgctgagcg gtacaggaaa ctgggttttg atgtacaatg gattccggga gacagctttg
     3361 gacgtgcagg acatttggtt gctacaagat tggctgccaa aggtccgaaa attcttatga
     3421 tcggccacct ggataccgtt tttgaacttg acagcccctt tcaggaatat accatgatcg
     3481 acgatcactt gatgaagggc cccggagttt tggacatgaa aggtggtaat gtagttatgt
     3541 atttagctat taaagctctt tatgagttaa aactcttgga caatttatcc ataaaagtat
     3601 ttttggttgg tgatgaggag ttgagcggtg aacctattgc cttatcaaag aaagatctca
     3661 tcgaagccgg gaaatgggct gatattgccc ttggatatga agatggtgat ggaactttcg
     3721 accatgccaa tatttcccgc agaggttcag ccgactggac tcttaaagta aaaggaatac
     3781 ctgcgcacag ttcacaaatt tttcaacctg aagttggcgc aggtgcaatc tatgagacat
     3841 cgagaatcct tcatgaattt tataaacaat tgagtaatga agaaaacttt acttttaacc
     3901 caggtctcat tagtggaggt aattcgttaa cattccatga agagacaaat aaatctgaag
     3961 cttttggaaa aaacaatatt gtgtcggccg aggtgattgt cacaggcgat atgcgtgctg
     4021 tctccatgga acagctgatc cgaaccagga gaatcatgca ggaaatagtc agcaattccc
     4081 tacctaatac ctcagcaacc atcgaatttg gtgatgacgg gtatccgccc atgactatga
     4141 cagaaggcaa cattcagttg ctgggcctgt tcagtaaggt aagtcaggac cttgggtatg
     4201 gtacagtgca tccggtaaat cccagaaatt caggtgctgc cgatatttcc tttgtggcag
     4261 aagatgtttt catggcgatg gatgccattg gattgtgtgg taccggcggg catactatag
     4321 aagagacagc tgatatatca tgcctgccga aggaagccaa acgcactgct atccttcttt
     4381 accggttaaa agacataaaa ttttaatgac catggaagaa tggtcagaaa ctaaagccaa
     4441 tgaaatcatt tctgaactta tgctcagcaa tgatgctttt agcaaatggc ttggagtgcg
     4501 tgttgatttg gtaaaggaat cttctgtcga aatatcatgt aatattactg aaactatgac
     4561 caatggattc catgttgcac atggcggaat tgtcttttca ctatgtgaca gtgccctggc
     4621 ttttcactcc aattcctggg gcagaaaagc tctttctata gaaacctcca tcaatcattt
     4681 atccccaatc agagtgggtg ataggataac tgccaaaacc tccataataa aagttgggaa
     4741 atcaatcggt aatttcctga ttgaggcata taatcaggat gataaaaaag tggccatcat
     4801 gaagggtatg gtgttctacc ttggagatcg ttgggcatga ttccacatta accgtgaagt
     4861 cttcccttcc tcatcaatag tatttcgtcc gattccctgc gatcaggatc tggtacacaa
     4921 caatccaccg ggcacactgc tgcacattgt ggttcttcat ggaagccaac acattcggta
     4981 cacttgtcag gtacgatata ataataatcg tccgaaacag gagacatcaa ttgatcatat
     5041 cttaccactg acccatcttc cagtgtataa tcaccaacaa ctgtggtgcc atctttcatt
     5101 ctccattcaa caccgccttc gtaaattgca ttgttaggac attccggctc acatgcacca
     5161 caattgatac attcatccgt tattataata gccatttatt aaagtcaaat tttaatcaaa
     5221 gatacaacat atacaaataa cactttgttt acttatcatt cctaagcgta aagtaagctg
     5281 ctgttgtata aaaatttcta aagaaaggta gtttcgtcaa aaaaccggga acctttctgg
     5341 ttctaagctt aaacttatat tcataaatag gattgataag ccaaaaggat gtatcaataa
     5401 tattgaagtg ttggcgagca atagcgttga atcgttcaat actgataccg gtggctttca
     5461 gttccatcag ctcttttact gtactttccg cttctcctgc catctgtaac aaggatctgt
     5521 ataaaggtcc cggtagtaaa tgtatccagg gtattttgga aaggatttta cttcttaaaa
     5581 tctgttggtg accaccgaat ggcatatacc agggtggaaa tgcaaagaat aatgtcccgc
     5641 ccggtttgga tagtttcttc aattcaggaa ttattttctc ctgatccggg atatgctcta
     5701 ttacatcttt aaggataatc aggtcaaaag gctccagatt gtctctttgc ggat
//
LOCUS       DAOQMC010000154         5723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6169_length_5723_cov_5.393613, whole genome shotgun sequence.
ACCESSION   DAOQMC010000154 DAOQMC010000000
VERSION     DAOQMC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6169_length_5723_cov_5.393613"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1041)
                     /locus_tag="PKV31_07030"
     CDS             complement(166..1041)
                     /locus_tag="PKV31_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92647.1"
                     /translation="MDKIALRNGILAFILVLLLMPIGHAIMILNEVLLGDAKYYGAIA
                     MGFLGVALIVIGLLKNGNPTFATLMGFLGGVLVWTGWVEFSFMWVAEKNNVANLMENG
                     NIVTKREYLVMLSSMGLLCTTVLFYLFSRSNCTFFIWFQKVLGFRNQILTQTGYKKPF
                     SIIVFTETIMLLWFFYILLLAIYDDHIAGDRHWITYTVAWGSLFWSLYLINRLVRIRS
                     FDYAVRYAVPTVIIFWNFVEILGRWDLFKEIWIHPMEYWLEVLLLCLAFVTLMILFIV
                     NPTFHRKGNKETAAT"
     gene            1205..2491
                     /gene="fucP"
                     /locus_tag="PKV31_07035"
     CDS             1205..2491
                     /gene="fucP"
                     /locus_tag="PKV31_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HNB92648.1"
                     /translation="MANPTDTTPTTSSSTKGYLLPLVFITSLFFFWGLIHNIDPTLIA
                     HLKKTYTLTNLQSSLIDFSVFIAYFLMAIPAGLMMRRWGYKAGIVFGLLLFAGGAFLF
                     LPAADTHQYVYFLGALFVVASGLTFLETAANPYVTVLGPPDTSSFRLNLSQSFNGIAA
                     VIAPIIGKYTILTHAADVSNEDIASMDATAKQEFIRQASESVKGPFMILGFIILAVAI
                     IFLFLKLPEITEEKSVKKRSFLEVLKVKHTRNAVIAQFFYVGAQICFASFFLVYAEKA
                     SGLDHLRASDYLAAYGLLFMLGRILGTIILKFVAAPRLLFIYALSASALTVIAIFGYG
                     AYTIYCMLGIAFFLSIMFPTIFALGVAGLGEDTKTASSLIVMSIVGGAILPLGLGYLA
                     DITGNIQYGYLVPLICLLVVALFGLRGYKPQQGLVT"
     gene            complement(2764..4038)
                     /gene="aroA"
                     /locus_tag="PKV31_07040"
     CDS             complement(2764..4038)
                     /gene="aroA"
                     /locus_tag="PKV31_07040"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNB92649.1"
                     /translation="MKSIIKPSRLAGTITAPTSKSSMQRACAAALLNKGCTIIRNPGL
                     SNDDKAAVEIIQALGADVRISKGEMIVKSNGINPISKEIHCGESGLGIRMFTSIAALS
                     EKEITITGSGSLMSRPMDFFDEILPQLGVKVESNGGKLPLKVRGPLVPGNITVDGNLS
                     SQYTTGLLMAYAAAGAKGVSIHVRNPTSKPYIDLTLEVMRSFGMEVPENRHYEEFVFH
                     GKPEKNTAPGQCQYTVESDWSGGAFLLVAGAIAGPITVCGLRQESTQADKAIMKALKS
                     AGAGIKVHEDKIQIYPSELKGFLFDATDCPDLFPPLVALAAYCRGETIIRGAERLTHK
                     ESNRALSLQSEFGKMGVVIDLAGNNMTIHGSCEVQGAEVSSWHDHRIAMACAVAALKA
                     KGGMTITDAEAVGKSYPGFFEDMRTLGADLEA"
     gene            complement(4157..4279)
                     /locus_tag="PKV31_07045"
     CDS             complement(4157..4279)
                     /locus_tag="PKV31_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92650.1"
                     /translation="MTERESMEFGGYCKNKDHLLWNQQQGEMKVRNGQTISTAS"
     gene            complement(4279..4779)
                     /locus_tag="PKV31_07050"
     CDS             complement(4279..4779)
                     /locus_tag="PKV31_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNB92651.1"
                     /translation="MNERPKIKLEQTSIDKTFEILGWTSVIAIWILTITNYSNLPEII
                     PTHYNGAGQPDGFVGKANILTLPLIATVLFVGLTILNKYPHIFNYPTGVTQENALRLY
                     TNATRMIRYLKFIIVAIFGLITFKTIQNVNGEAKGLGNMFLPVSMGLIFIPLAYFLLK
                     SFRTTK"
     gene            complement(4837..>5723)
                     /locus_tag="PKV31_07055"
     CDS             complement(4837..>5723)
                     /locus_tag="PKV31_07055"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNB92652.1"
                     /translation="KGLPWLKKSGDITGLPVTVEVANAKQVQDALAFEIDVLWIGART
                     TVNPFSVQEVADALRGVDIPVLIKNPVNPDPELWSGAVERIARAGINRIGLIHRGFTS
                     YGNSEFRNAPMWHLAIDMKLRYPDMLFINDPSHICGRRDNLREVMQKAIDLDYDGFII
                     ESHCDPDHAWSDASQQVTPETLSLMLDQIKWRKEDTSSAEFHIALEKLRNQINQLDDE
                     LMQILSRRMKIADQIGRYKKDNNITILQTTRWNEVMDRAVSGGAGLGLSKEFILRYFD
                     AVHIESINHQKRIMDGEE"
BASE COUNT         1488 a         1397 c         1099 g         1739 t
ORIGIN      
        1 atatatttct ttagattcat tttggtctat ttaaatattc attttcgtat tatttatcta
       61 cctacttcag tgtgaccacg ctaaccacca tttaccacaa gcctccacac ctgcccgcta
      121 agcctattct atccaataat cagaaattta accggccttc cattatcagg tcgctgccgt
      181 ttccttatta ccctttcggt gaaatgtagg attgacaata aacaaaatca tcaatgtaac
      241 gaaggccaaa cataacaaca aaacttccaa ccaatactcc atcggatgaa tccaaatctc
      301 tttgaaaaga tcccatcttc caagaatctc tacaaaattc cagaatatta tgaccgtagg
      361 cactgcatac cttacggcat aatcaaagga acgaatcctt acaagtcgat tgatgaggta
      421 aagagaccag aacaatgagc cccatgcgac agtgtaggta atccagtggc ggtcaccggc
      481 tatgtgatca tcgtatatgg cgagtaaaag aatatagaaa aaccagagca gcatgatagt
      541 ctctgtaaaa acaataatgg aaaatggctt tttataaccc gtttgcgtca gaatttgatt
      601 tctgaaaccc aaaactttct gaaaccagat gaaaaaagta caattggacc tggaaaaaag
      661 atagaacagc accgtagtac atagcagacc catggatgaa agcatgacaa ggtattcccg
      721 ctttgtgaca atatttccat tctccatcag attggcaaca ttatttttct cagcaaccca
      781 cataaaggaa aattccaccc acccggtcca cacaagtaca ccgcccagga agcccattaa
      841 tgtggcaaaa gtcggattgc cgttcttaag caagccaatt acaatcaaag ccacccccag
      901 aaagcccatc gctatagctc cataatactt ggcatcacca agcaatacct cgttgagaat
      961 catgatggca tgccctatag gcatgagtaa aagtaccaga ataaatgcca gaatcccgtt
     1021 tctcaatgca atcttatcca tatcctttaa taatattaat gagcaaagat ataatttata
     1081 tttagtctaa ataacattgg tgttgttttt tatattttgg ttttgccaca aagttcccat
     1141 cgggataata aaatgatacc aatttcgacc taatgattat ttttgataag ataaaacctg
     1201 acaaatggct aaccctaccg atacaactcc cacaacatct tcttcaacca agggctatct
     1261 gctgccttta gtctttatca caagtctttt ctttttttgg gggctaattc ataatattga
     1321 tccgacccta atcgcccatc ttaagaaaac ctacaccctg actaacctcc aatcatccct
     1381 gatagatttt tcggtcttca tcgcttattt tctgatggct ataccggcgg ggctgatgat
     1441 gcgccgatgg ggctataagg ccggcattgt gtttggttta ttgctctttg ccggtggtgc
     1501 ctttctcttc ctgcctgctg ccgataccca ccaatatgtg tattttctgg gggcactttt
     1561 tgttgtggcc agcggcctta ccttcctgga aaccgcagct aatccttatg tcactgtgct
     1621 gggtccaccc gatacctcct ctttccgact caatctgtct cagtcattca acggcatagc
     1681 ggcagtcatt gctcctatca tcggcaaata caccatcctc acacatgctg ccgatgtcag
     1741 taatgaagac attgcatcga tggatgcaac ggccaaacaa gaatttatcc gacaggcttc
     1801 tgaaagtgta aaggggcctt ttatgatatt gggcttcatc atcctggcag tagccatcat
     1861 cttcctgttt ttgaaacttc ccgagatcac agaagaaaaa tcagttaaaa agagaagttt
     1921 tctggaagtt ttgaaggtca aacatacccg aaatgcggta attgcccagt ttttttacgt
     1981 aggagcacaa atctgctttg ccagtttttt cctcgtgtat gcagaaaagg cttccggtct
     2041 tgaccatctc agggcaagcg actacctggc cgcctatggc ctgcttttca tgcttggccg
     2101 gattttgggc actatcatat tgaagtttgt tgctgccccc cggctattat tcatttatgc
     2161 cctgtcggct tcggcattga ccgtcatagc tattttcggt tacggtgcct acaccatcta
     2221 ctgtatgctg ggcattgctt ttttcctatc catcatgttt ccaactatct ttgcccttgg
     2281 tgtcgcagga ttgggcgagg acaccaaaac cgcttccagc ctaatcgtca tgtccattgt
     2341 gggcggggct atcctaccct taggcttggg ctatctggcc gatataacgg gcaatattca
     2401 atatggttat ttggttcctc tgatctgcct tctggtagtg gctctgtttg gattgcgggg
     2461 ttataagccc caacagggat tggttacata aaatctttcc ttttctcctc ttcacgcctt
     2521 ccttccaaac tcacactaca gcatgggtat actgaatata tttagataat ccgttgcttt
     2581 acaaagcttt ctttatccgg aaactttctt gtctttctat tttgcgtata cttctttaaa
     2641 aatctgacgg tcggtcggta ttggaaaact acccggtatg gctctgcgaa aattcgattg
     2701 aaatatatga atctaccccc cgaataatta taaatagcag ggaatccttg tcaaatcaaa
     2761 attttatgcc tccagatccg cccccaatgt cctcatatcc tcaaaaaagc cgggatagga
     2821 tttgccgacg gcttcggcat ccgtgatggt catacctccc ttcgccttta atgcagcgac
     2881 cgcacacgcc atggcgattc ggtgatcatg ccaggaagat acctctgctc cttgcacctc
     2941 acaactgcca tgtatggtca tattgttgcc ggcaaggtca atgacaacac ccatcttacc
     3001 aaattcggac tgtagtgata atgcacggtt gctttccttg tgcgtcagtc gttcggcacc
     3061 tcgtatgatg gtctcacccc ggcaatacgc cgccaatgcc accaacggtg gaaaaagatc
     3121 gggacaatcc gttgcatcga acaggaagcc tttcagttct gatgggtaga tctgtatctt
     3181 gtcttcatgg acttttattc cggctcctgc tgattttaag gctttcataa ttgccttgtc
     3241 ggcctgggtt gattcctgcc gtaacccaca tactgtaatc ggtccggcta ttgctccggc
     3301 aaccaacaag aaggctcctc cactccagtc cgattccacg gtatattgac attgtcccgg
     3361 ggccgtgttc ttttccggtt ttccatggaa tacaaattct tcataatgcc tgttttcagg
     3421 tacttccatg ccaaaagacc gcatcacctc taaggtaagg tcgatgtatg gcttgctggt
     3481 tggatttctg acatgaatgg atacgccttt tgctcctgct gctgcatagg ccatcaacaa
     3541 tcccgtggta tactgcgaac taagattgcc atctacggtg atatttccgg gaaccagcgg
     3601 ccctcgtact tttaagggaa gtttgccgcc gttgctttcg accttcactc ccagctgcgg
     3661 cagtatttca tcaaagaagt ccatcggtcg gctcatcagg ctgccacttc ctgttatggt
     3721 tatttctttt tcagataatg ccgctattga agtaaacatg cggataccca ggccgctttc
     3781 gccacagtgt atctccttac ttatcggatt tataccgtta cttttgacaa tcatttcgcc
     3841 ttttgaaatt cggacatcag caccaagtgc ctgaataatt tccactgccg ctttatcgtc
     3901 attactcaag ccaggattgc ggattatagt gcaacctttg ttcaatagtg ccgctgcaca
     3961 agcacgctgc attgagcttt tcgaagtagg tgctgtgatg gtaccggcca gtcgcgatgg
     4021 ctttatgatt gatttcatcg attcttgttg actgttattg ttgttatcgt ttatttcaaa
     4081 ggtaaatgtt tttggcgttg gtcagcatct tcatccttca aagattgaat tgattgcttc
     4141 gtaactatcc acatattcag gaagcagtag aaatagtctg tccattgcgc accttcattt
     4201 ctccctgttg ctgattccat aacagatggt ctttattctt acagtaccca ccaaattcca
     4261 tgctttctcg ctctgtcatt attttgtcgt cctaaatgat tttaataaaa aatacgccaa
     4321 aggaataaaa atcagtccca ttgacactgg taaaaacata tttcctagtc cctttgcttc
     4381 accgttcaca ttctgaattg tcttgaaagt aattaatccg aaaatcgcaa caattatgaa
     4441 ttttaaatat ctaatcattc ttgttgcatt tgtatacaac cttaatgcgt tttcttgagt
     4501 tacaccggtt ggatagttaa atatgtgagg atacttattt agtattgtca gcccaacgaa
     4561 tagaacagtt gctatcaaag gtagcgtcaa aatgttcgct ttcccaacaa acccatccgg
     4621 ttgtcctgca ccattataat gggtaggaat aatttcaggt aagtttgaat agtttgtaat
     4681 agtcagaatc caaattgcaa taacagaggt ccaaccaaga atttcaaagg tcttgtcaat
     4741 cgatgtctgt tccaatttaa tttttggcct ttcattcata atcataccat tatataccga
     4801 atatccagga ttgggttata tctaccatgt tacccatcac tcctctccat ccattatacg
     4861 tttctgatgg ttaatacttt cgatgtggac tgcatcaaaa taccgtagga taaattcttt
     4921 gcttaaacca agacctgctc caccggatac cgctcggtcc atcacttcat tccaacgtgt
     4981 cgtctgcaga atggtgatat tgttatcttt cttgtaacgg cctatttgat cagctatctt
     5041 catcctgcga ctgagaatct gcatcagttc atcatccagc tgattgatct ggttgcgaag
     5101 tttttccaga gcaatatgga attccgctga gctggtgtct tccttccgcc atttgatttg
     5161 gtccaacatc aggctcagag tctcaggtgt cacctgttgc gaggcatcgc tccatgcatg
     5221 gtcaggatca caatgacttt ctataataaa accatcgtag tccaggtcta ttgccttttg
     5281 catgacttcc ctgagattgt cacgacggcc gcagatatga gatgggtcgt tgataaacaa
     5341 catgtccggg tatcgcaact tcatatcaat agcaagatgc cacatgggtg cattgcggaa
     5401 ttccgagttg ccataactgg tgaatcctct gtgaatcagt cctattctgt tgatgccggc
     5461 ccttgctatc ctttccacag cgccactcca aagttcgggg tcaggattga cgggattttt
     5521 aatcaatacc ggtatatcca ctcctcgtaa ggcatccgcc acttcctgta cactaaaggg
     5581 attgactgtt gtcctcgctc cgatccagag cacatcaatc tcaaaggcca atgcatcctg
     5641 aacctgtttg gcattggcta cctctacggt cacaggcaac cctgtaatat ctcccgattt
     5701 cttcagccaa gggaggcctt ttg
//
LOCUS       DAOQMC010000155         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6197_length_5713_cov_4.905090, whole genome shotgun sequence.
ACCESSION   DAOQMC010000155 DAOQMC010000000
VERSION     DAOQMC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6197_length_5713_cov_4.905090"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PKV31_07060"
     CDS             <1..1164
                     /locus_tag="PKV31_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92653.1"
                     /translation="INPTCFGGSDGAIFPEIITPGQEPYYFQWSDDTHDVILDSLKAG
                     KYYVTIYDNANCMLTLSATLINPSRMNVDYILTQPDGPGKPYGSIKVFLNGGAPPYKV
                     SFQGVDYLGGNEILIDNIPPGNYTAYAKDSKGCSTPVDFQINQFECLLTGNVITVDEP
                     DCYGDKNGQLCVMYQNNFGSVSIEWSNGATTACISNLIAGEYAVVLRDTLGCMVVLES
                     TVKQPNFITLENITINPGTNHFDGSISLFVIGGTPPFTYTWTKNGEYFANTRNINQLN
                     EGTYQLSVVDSKGCTALFEAFRLTPSKSFSNYETGIKVYPNPVYENLIVETRNYTVIH
                     SIDYISMNGKKVRLPFATTDKGAVLDVGTLPQGPFVLSVVTNEGIANVKLVKM"
     gene            complement(1220..2857)
                     /locus_tag="PKV31_07065"
     CDS             complement(1220..2857)
                     /locus_tag="PKV31_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB92654.1"
                     /translation="MRKVKIIGIVLVFAFVGMGAAVYNNDKLFEITKNLEIFSNLYKE
                     LNTYYVDDIDPNKLMKVGIDAMVETLDPYTNYFSETQIETWRYLSEGKYEGIGANVKN
                     IGDEITVIEPYEGYPAYEAGLKAGDVILSIDGQSTKGKNSNDLGVLLRGVPGSSVQLE
                     IKRPGVKEIKTFKINRGEIKMSNVPYSGIVRDHVGYVSLSTFTQDAAKNIGDAIREMK
                     KNDPQLKGLVLDLRDNGGGLLIEAVNIVNLFVGANVEVVSTRGKVRDWDKVFSTRGPA
                     LDEKLPLVVLINKQSASASEIVSGSIQDLDRGVVIGQRSFGKGLVQNTKDIGYNSKLK
                     LTTSKYYIPSGRCIQSVSYKNGEPVDIPDSQRHVFRTKGGRKVLDGGGVTPDIYVAPD
                     SGDAPIKALLDDFVVFNYVTKYMIAHPNIAPADQFVFNDFDDFLKYVESNNYPFTTAS
                     EKKFEELTNSARKDEFYAPLQSDLSALKMKIQKEKRNVLIKHKTQINTFITKDIVNRA
                     YGQHGKIINSINHDREVSDAINLLNDPTRYNKILKGK"
     gene            complement(2975..3475)
                     /locus_tag="PKV31_07070"
     CDS             complement(2975..3475)
                     /locus_tag="PKV31_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92655.1"
                     /translation="MRTVLSLVVLCLPCWLSMTVLHPFHVSNTEFNYNATDKNLEVSC
                     RIFTDDFEKALGMYFKEKADFTDKKLSGKMEIMVRDYMKTHLAIKIDNKRLALSCLGF
                     ELDHEAVNVYLEHPLALPPKKIEVQNTLVYEAYEDQVSVVHFIVNGQRKSSKLDYPDK
                     VLHFTF"
     gene            complement(3472..>5713)
                     /locus_tag="PKV31_07075"
     CDS             complement(3472..>5713)
                     /locus_tag="PKV31_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB92656.1"
                     /translation="ESLEMYPFKVLINQGVQSVMIGHLVVPALDSMQNRPASLSKPII
                     TSWLKEKLGFEGLVITDALDMKGVTKNFQKGEIEVLALKAGVDVLLLSEDVPVSIAAI
                     KKALKDGKLTAERIDESVRKILEAKYRFGLTGSVNISTANLASDLVTGEARALNQKII
                     EQAITMVKNADGLVPIGNIEKKISTLAIGASTKTPFQQQVDAYCKASHFFFNKETSSS
                     LSEKLSGMDLVIVGITGMNNSLKDNFRISKDAVALVNQLSKKTKVIVVVFGNPYGLSN
                     FVSPSTVICAYNDDALSQSLGIQAVFGGLPINGKLPVTALPYPFESGLQVSEATRISF
                     GEPESVGMDSGTLAKLDELARDLVSRQAAPGCEMLVIRDGKVVYNKQFGRYTYDNRSH
                     QVEQYTMYDLASITKIAATTLGIMKLYEQGKMDVYKFLGSYLPELKGSNKEFMVIRDV
                     MAHRAGLFPWLPFYKETLVNDGFRTRTSAMIYDSKKSDEYSIKVANRLYMRSDFINTL
                     WNEIIRNQNLQSNDYKYSDLGFIMLRKAVENITKEPMDKYLESTFYKPMGLYSMTFKP
                     LEKFQKSDIAPSEDDRYFRQQVLQGDVHDMAAAMFGGVSGHAGLFSNSVHLGTLMQML
                     LNGGSYGGKQYLKAETIKLFTTRHPADTRRGLGFDMPQTDWSMIQNVTAKASRNTFGH
                     SGFTGTVVWADPQSRLIFVFLSNRTYPTMDNNLLERRNYRIKAHAITYEAISKYDHTD
                     DLNASL"
BASE COUNT         1462 a         1279 c         1258 g         1714 t
ORIGIN      
        1 atcaacccca cttgctttgg tggctcagat ggagcaatct tcccggagat aatcacaccg
       61 ggccaggaac cctattattt ccaatggtcg gatgacaccc atgatgttat cctcgacagt
      121 cttaaagccg ggaagtatta cgtcaccata tacgataatg ccaattgtat gctgacattg
      181 tcggctaccc tgatcaatcc gagccggatg aatgtcgatt atatacttac acagcccgac
      241 ggtccaggca aaccatatgg ttcgattaag gtctttctca atggaggggc accaccctat
      301 aaggtatcct ttcaaggggt agattatctg ggtggaaacg aaattttaat agataatata
      361 cctccgggta attatactgc ctatgccaag gattcaaagg gatgctccac cccggttgac
      421 ttccagatca atcagtttga atgtctgttg acgggaaatg ttatcactgt ggatgagcct
      481 gattgctatg gggataaaaa cggacagttg tgtgttatgt atcagaataa ctttggatca
      541 gtctccattg aatggtcaaa tggggcaaca acagcttgta tcagcaatct gattgcgggt
      601 gagtatgcag tagtgcttcg agacaccctc gggtgtatgg ttgtccttga atctacggtc
      661 aagcagccaa acttcattac gctggagaat atcacaatca atcccgggac taatcatttc
      721 gacggatcta tttcgctctt tgttattgga ggcacgccgc catttaccta tacctggact
      781 aaaaacggtg aatattttgc caatacaagg aacatcaacc aattgaatga agggacttac
      841 cagctcagtg tagttgactc taaaggctgt acggcgctat ttgaagcctt caggctgact
      901 ccttccaaga gtttttctaa ctatgaaaca gggataaagg tgtatcccaa tcctgtctat
      961 gaaaatttaa ttgtagagac acggaactat acagtcattc atagcattga ttatataagt
     1021 atgaatggta aaaaggtgcg gttgcccttc gccaccaccg acaagggagc ggtgcttgat
     1081 gtaggcacat tgccgcaggg gccttttgtc ctcagtgttg tcacaaatga aggtatcgcc
     1141 aatgtaaaat tggtgaagat gtagtaattc tatgcttgaa caatatgtaa aggcagaagt
     1201 cacatgactt ctgccttttt tattttcctt tcagtatttt attataccgg gtgggatcat
     1261 tgagcaaatt tatggcatca cttacttccc ggtcatggtt gatgctgttg attatctttc
     1321 catgctggcc atatgccctg ttgacaatat cttttgtaat aaaagtattg atctgggtct
     1381 tgtgcttgat cagcacattt cttttctctt tctgtatttt cattttaagt gctgacaaat
     1441 cgctttgtaa tggagcatag aactcatcct tcctggcaga attagtcaac tcttcgaatt
     1501 tcttttcaga agccgtggtg aacggatagt tgttggactc gacgtattta agaaagtcat
     1561 caaagtcatt aaaaacaaat tgatctgccg gtgctatatt gggatgggca atcatgtatt
     1621 tcgtgacata gttgaaaacg acaaaatcat caagtagcgc tttgattgga gcatcacctg
     1681 aatccggagc cacatagata tccggagtca caccgccacc gtcaagcact tttcttcctc
     1741 cctttgtcct gaagacatgt ctttgactat ccggaatatc gacaggttca ccatttttgt
     1801 atgaaacact ttgaatacac cgtccactgg gaatataata tttggaggtg gttagcttca
     1861 acttactgtt gtaccctata tccttggtgt tttgtacaag gccttttccg aaggagcgct
     1921 ggccgatcac aacacctctg tccaagtcct gaatcgaacc gcttacgatt tcagaagcag
     1981 aagcactctg tttattgatg aggacgacga ggggtaattt ttcgtccagt gccggtccac
     2041 gggtagaaaa caccttatcc caatccctta ccttacctct tgtgcttact acctccacat
     2101 tggcaccgac aaagaggttg acaatattga ctgcctctat aagcaagccg cccccattgt
     2161 ctcttaagtc gagtaccagg cccttgagct gtgggtcgtt ttttttcatt tcacggatgg
     2221 catcaccgat atttttagcg gcatcctgtg taaaggtcga cagagataca taaccgacat
     2281 gatcacgaac gataccagaa taggggacat tgctcatctt gatttcacca cggttgatct
     2341 tgaaggtctt gatttccttt acaccaggac gtttgatttc caactgtacg gaagaaccgg
     2401 gcacgcctct caatagaacg cctaggtcat tgctgttttt gcctttagtg gactggccat
     2461 cgatggaaag aatgacgtct ccggctttca ggcctgcttc ataggccgga taaccttcat
     2521 aaggctcgat gacggtgatt tcgtctccga tgtttttgac attggctccg ataccctcat
     2581 attttccttc agataaatat ctccaggtct ctatctgggt ctcgctgaaa taattggtat
     2641 aagggtcgag cgtctcaacc atggcatcga taccaacctt catcagtttg ttcgggtcga
     2701 tatcatccac ataataggta tttagttctt tataaaggtt ggagaaaatc tcgagatttt
     2761 ttgttatttc gaatagctta tcgttgttat atacggcagc gcccatgccc acaaaggcaa
     2821 atacaagaac aattcctata atttttactt tcctcatttc gcaagattaa tttttctttt
     2881 cacataaaac ggatatccga cttcttttga tatcagcaac gaattttttg accaattgtt
     2941 ttttgataca ggttgatcat agtcctgatc atatttaaaa ggtgaaatgg agtactttat
     3001 ccggatagtc cagcttgctg gattttcttt ggccattgac gataaagtgc actacactga
     3061 cctgatcctc ataggcttca tatacaagag tattttgtac ttcaattttt ttgggaggca
     3121 aggccagcgg gtgttccaga tacacattga ccgcctcatg gtcgagctca aatccgagac
     3181 aggatagagc cagcctttta ttgtcgattt tgatggcgag atgggttttc atataatccc
     3241 gaaccataat ttccattttc ccggacaatt ttttgtcggt aaagtctgct ttttctttaa
     3301 aatacatgcc aagtgccttt tcaaaatcgt ccgtaaagat cctgcaggac acctccagat
     3361 ttttatcggt tgcgttatag ttgaattcgg tgttgcttac gtgaaaggga tgtaagacag
     3421 tcatggacaa ccagcaagga agacaaagga cgacaaggct cagcacagtc ctcataagga
     3481 agcatttaag tcatcagtat gatcgtattt gctgattgct tcataagtga tggcatgggc
     3541 ttttatacgg taatttcgtc tttccagcag gttgttatcc atggtcgggt aggtcctgtt
     3601 ggacagaaat acgaaaatga gtctggattg ggggtcagcc cagactactg ttccggtgaa
     3661 tccggaatgc ccgaatgtat tgcggcttgc ttttgctgtt acattctgaa tcatgctcca
     3721 gtcagtctga ggcatatcaa atccaaggcc acgtcttgta tcggccgggt gtcttgttgt
     3781 aaacaacttg atggtttcgg ctttaagata ttgtttgcct ccgtaactac ccccattaag
     3841 gagcatctgc atcagtgtgc ccagatgtac cgaattgctg aatagtcctg catggcctga
     3901 aactcctcca aacatcgcag cggccatatc atggacatcg ccctgtaaca cctgttgtct
     3961 gaaatagcga tcgtcctcgg agggggcaat atcgcttttc tgaaactttt caaggggctt
     4021 gaaggtcatg ctgtaaagac ccattggctt gtagaatgtg gattcaagat atttgtccat
     4081 cggctctttt gtaatattct caactgcttt ccggagcatg ataaatccca ggtcggaata
     4141 tttataatca ttgctttgca ggttttgatt gcggatgatt tcattccata gggtattgat
     4201 gaagtcgctt cgcatataca gacggtttgc aaccttaata ctgtactcat cacttttctt
     4261 tgaatcgtag atcatggcag aagttctggt cctgaacccg tcattgacca atgtctcctt
     4321 ataaaatgga agccagggaa acaatcctgc cctgtgagcc attacatccc tgataaccat
     4381 gaattccttg tttgatcctt tcaattcagg caggtaagat cccagaaact tgtaaacatc
     4441 cattttcccc tgttcgtata gcttcatgat tccaagcgtg gtagctgcta tctttgtaat
     4501 ggaagcaagg tcgtacatgg tatattgctc cacctggtgt gaacggttat cataagtata
     4561 tcgtccgaat tgcttgttgt aaaccacctt gccatcgcgg atcaccagca tctcacaacc
     4621 gggtgctgcc tgtctgctta ccaggtccct ggccagttcg tccagcttag cgagcgtacc
     4681 ggagtccata ccgacgcttt ccggttcgcc gaagcttatt ctggtcgcct cagacacctg
     4741 gagcccactt tcgaaaggat atgggagggc tgtgactggc aatttaccat tgatgggcag
     4801 accgccaaat acggcttgaa tccccaaaga ctgtgataag gcgtcatcat tgtaggcgca
     4861 gataaccgtt gaagggctta cgaagttact tagcccataa ggattgccga agaccacgac
     4921 tatcaccttg gtcttttttg acaattggtt gacaagggcc actgcgtcct tgcttatcct
     4981 aaaattatcc ttcagggagt tattcatgcc ggtaattccc acgatgacaa gatccatacc
     5041 ggataatttt tctgacagag atgagcttgt ttccttgtta aagaagaagt gacttgcctt
     5101 gcaataggca tcaacttgct gttggaaagg agttttggtg gaagcgccta tggccagtgt
     5161 ggatatcttc ttctctatgt taccgatggg gaccagccca tcggcattct tgaccatggt
     5221 gattgcctgt tctatgattt tttgattgag ggcacgagct tctccggtga ctaagtcgga
     5281 ggcaaggttg gccgtggaaa tattgacgga cccggtcaat ccgaatcggt acttggcctc
     5341 caggattttc cgaacacttt catcgatccg ttcagcggtc agttttccat ccttcagtgc
     5401 ctttttgatt gctgcaatgg agacaggaac atcttctgac agcaggagta catccacacc
     5461 tgctttcagc gctaatacct caatttcacc tttctggaaa ttctttgtga cccccttcat
     5521 atcaagggca tcggtaatga ccaaaccctc aaacccaagt ttttccttca gccatgaagt
     5581 aatgattggt ttggacaagg atgccggtct gttctgcatg gagtcgagag ccggaactac
     5641 caggtgtcca atcattacgc tttgaacacc ttgattgatc agcaccttga agggatacat
     5701 ctccagggac tcg
//
LOCUS       DAOQMC010000156         5664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6293_length_5664_cov_4.546087, whole genome shotgun sequence.
ACCESSION   DAOQMC010000156 DAOQMC010000000
VERSION     DAOQMC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_5664_cov_4.546087"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /gene="ppk1"
                     /locus_tag="PKV31_07080"
     CDS             <1..1627
                     /gene="ppk1"
                     /locus_tag="PKV31_07080"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNB92657.1"
                     /translation="NNSALYLAIHMQLEGVANSGDQYAIVKIPSDHLPRFIEIPPGVQ
                     GQRDIIFIDDIVRHSISWLFPGYNIHDTYSIKLTRDAELYIDDEYSGDLATKIRASLN
                     KRSVGPAARLVYDSNMPLKFRNYLKGVFELENIDLFPEGRYHNKKDFFSFPDLGIKDI
                     VAQKMEAVRYKKLEEDSTYWDEIKSRDHLLYFPYNSYDPVIKMFELAARDPDVTHIKL
                     IQYRVAEDSKIMEYLIEAAENGTQVSVFIEIKARFDEEANLRWGEKLARAGIKVHYSL
                     PGIKVHSKLALIRRIEDDKPNFYSYLGTGNFNEDTANLYTDFGLFTADVRYTKDVMKV
                     FNYIETGKKPASAFNHLLVGQFNLRSGLEELIRYEINEAQHNRPAKIFIKMNSLQDKA
                     MIDLLYHASQQGVKIKMIIRGICSLVPGVSGVSENIKGISIVDRYLEHARVFIFHNGG
                     NEKVFLSSADWMERNLSYRVETAFPIFNPELKSEIHRLMMIQWEDNMKARTLKHKHVN
                     EYVKNDGDFPNRSQVESYYFIKRREELSMPIED"
     gene            1676..3022
                     /gene="purB"
                     /locus_tag="PKV31_07085"
     CDS             1676..3022
                     /gene="purB"
                     /locus_tag="PKV31_07085"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNB92658.1"
                     /translation="MSNLKAISPIDGRYSRQTVELSDYFSEYALIRYRVWVEIRYLLA
                     LQTSPVGNFGLSEEQVKIIRSIGDEFSEDDSLRIKELESITNHDVKSVEYFIKEQLDT
                     EGLSFIREWVHFGLTSQDINNTSFPLMIADAWKNVLGPQLEDLIKIVRDTGMSLVHIP
                     MLARTHGQPASPTTLGKEILVFAERLDNQMKLLKHIPWQGKFGGAVGNMNAHYIAYPG
                     FDWQEWADKFLTSIGLERQHTTTQIEHYDMLAAHFQGITRINNILTDLCRDIWTYISY
                     EYFTQKLKKDEVGSSAMPHKVNPIDFENAEGNLGMANAFFGFLAGKLPVSRLQRDLTD
                     STVLRNVGVPYAHTLIAIKSIVKGWNKLVVNENKIREELQQNSIVLAEAIQTILRREH
                     FDGAYEALKSLTRGRTSISREYLLSFVDSLDVNDEVKAELRKLEVEKYIGGWTPNK"
     gene            3697..4746
                     /locus_tag="PKV31_07090"
     CDS             3697..4746
                     /locus_tag="PKV31_07090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNB92659.1"
                     /translation="MIETKAYAAMSSDSPLAPWSLERRLPGPYDVQIEILYCGVCHSD
                     LHTARNEWHDTKYPVVPGHEIIGRVVAVGIDVVDFQTDDIVGVGCMVDSCSQCESCRE
                     GLEQYCEHGSTGTYNSKERDGSGVTYGGYSGMITVREDFVLRIPGNLPLTGVAPLLCA
                     GITTYSPLRHWKVGKGMKVGILGLGGLGHMGVKLAVAMGAEVTMLSHTATKAEDAERL
                     GAHHFILASDEGQMRSVRSSFDLILDTVSAVHDYNSYLKLLKRDGTMVCLGGPPVGPG
                     IESFNLIGKRRSIAGSLIGGIRETQEMLYFCAEHNIVSDVEVIRMDYINEAYERMLKG
                     DVRYRFVIDMASLKE"
     gene            complement(5428..>5664)
                     /gene="ssb"
                     /locus_tag="PKV31_07095"
     CDS             complement(5428..>5664)
                     /gene="ssb"
                     /locus_tag="PKV31_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB92660.1"
                     /translation="RYTNNDGKVVETATWHNLIAWGRPAEIAEKYLTKGREITVSGKL
                     VNRSYIDKNGNKKYVTEIEVNDFHIFGRTSNSVG"
BASE COUNT         1670 a         1028 c         1338 g         1628 t
ORIGIN      
        1 gaacaattcg gccttgtatc ttgcaatcca catgcagttg gagggtgtcg ccaatagtgg
       61 tgaccagtat gccattgtta agataccatc agatcactta cctcgtttta ttgagattcc
      121 acctggtgtt caagggcaac gcgatattat ctttattgat gatatcgtac gacatagtat
      181 ttcctggctt tttcccggat acaatatcca tgatacttac agtataaagc tcacacgtga
      241 tgctgaattg tatatcgatg atgagtattc aggtgatttg gcgacaaaga tcagagcaag
      301 tcttaataag aggagtgtcg gtcctgctgc gcgattggtg tatgattcca acatgccctt
      361 gaaatttcgc aattatttga aaggtgtttt tgagcttgag aatatagatc tgtttccgga
      421 aggcaggtat cacaacaaga aagatttttt tagttttccc gatttgggaa tcaaggatat
      481 tgtagcacag aaaatggaag ctgtgagata caagaagctg gaggaggact ccacttattg
      541 ggatgaaatc aaatccaggg atcatcttct ttactttccc tacaattcct atgatcctgt
      601 cataaagatg tttgaattgg ccgccaggga tcctgatgtg acacatatca agcttattca
      661 atacagggtg gcggaggatt ccaagattat ggagtatttg atcgaagctg cggaaaatgg
      721 tactcaggtc tcggtattca tcgagatcaa agctcgtttt gatgaagagg ctaatctgcg
      781 ctggggcgag aagctggcac gggcagggat caaggtacac tacagccttc caggcatcaa
      841 ggtgcattcc aagcttgcgc ttatacggcg gattgaggat gataaaccaa acttttacag
      901 ctatctcggt accggaaact tcaatgagga tacagctaat ctatacaccg attttggact
      961 ttttactgct gatgtacgat acaccaagga tgtaatgaag gtgttcaatt atattgaaac
     1021 aggcaaaaag ccggcatcgg cctttaacca cctgctggtc gggcagttta atctcaggag
     1081 tgggctggaa gaattgatcc gatatgagat caatgaggca cagcacaaca ggcctgccaa
     1141 gatattcatc aagatgaaca gcttgcagga taaggctatg attgacctgc tttatcatgc
     1201 atcacagcag ggagtcaaga taaagatgat tatcaggggc atttgttctc ttgttccggg
     1261 tgtatccggt gtaagcgaaa atatcaaggg gatcagcatt gtggatcgat atctggagca
     1321 tgcccgtgtc tttatcttcc ataacggagg caatgaaaaa gtgtttcttt cctctgctga
     1381 ctggatggaa cgaaatctta gttacagggt ggagacggct ttcccaatat ttaatcctga
     1441 attgaagagt gagatccatc gtttgatgat gatacaatgg gaggacaata tgaaggcaag
     1501 gacattgaag cacaaacatg tcaatgagta cgtcaagaat gatggtgatt ttcccaatcg
     1561 ttcccaggtt gaatcctact acttcatcaa aagaagggag gaattgtcaa tgccaataga
     1621 ggactgacga ttggaatata aatgtcatga ataataacag agaaaaaaag aaaccatgtc
     1681 aaacctgaaa gctatttcgc cgattgatgg tcgatacagt aggcagactg tcgaactttc
     1741 cgattatttt tcagagtatg ccctcatcag gtatcgggtt tgggtggaaa tccggtattt
     1801 gttggcgttg caaacttcac cggtgggtaa ttttggcttg agcgaagagc aggttaaaat
     1861 tatcagatcg ataggggatg agttttctga agatgatagt cttcgtataa aggagttgga
     1921 atcaatcacc aatcacgatg tcaagtcggt agaatatttc ataaaggagc aactggatac
     1981 agaaggattg tcctttattc gtgaatgggt acattttggc cttacttcac aggatatcaa
     2041 caatacttca ttcccattga tgatagccga tgcgtggaaa aatgttttgg gacctcaact
     2101 ggaagatttg atcaaaatcg tcagggatac gggtatgtcg ttagtgcaca tacccatgtt
     2161 ggccagaaca catgggcaac cggcatcgcc gacgacctta ggcaaagaga tactggtctt
     2221 tgcagagcga ttggacaatc aaatgaagtt gttgaaacat ataccatggc aaggaaaatt
     2281 tggaggtgcg gtaggtaata tgaatgcgca ttacatcgcc tatcctggat ttgattggca
     2341 agaatgggca gataaatttc ttacttcgat tgggcttgaa aggcagcaca ccacaaccca
     2401 gatcgagcat tatgacatgc ttgccgccca ctttcagggg attacacgga tcaataacat
     2461 actaaccgac ctctgtcggg atatttggac ttatatcagt tatgagtatt ttactcagaa
     2521 gctgaaaaaa gatgaagtcg gttcttcggc aatgccacac aaggtcaatc ccatagactt
     2581 tgagaatgct gagggaaatc tgggcatggc caatgctttc ttcggctttc tggcgggcaa
     2641 acttccggtg agccgattgc agcgtgacct caccgacagc actgttctga gaaatgtcgg
     2701 tgttccttat gctcacacgt tgattgctat taagagcatc gtcaagggtt ggaataagtt
     2761 ggtagtcaac gaaaacaaaa tccgtgaaga acttcagcaa aatagtatcg tgttggcgga
     2821 ggctattcag actatactcc ggagggagca tttcgatggc gcttacgaag ccttgaaatc
     2881 acttaccagg ggacgaacat ctatttcaag agaatatttg ctgagctttg ttgatagtct
     2941 tgatgtaaat gatgaagtaa aagctgaatt gagaaagctg gaagtagaaa aatacattgg
     3001 aggttggaca ccaaacaaat agtgacttct cgggcagcta ctgtgtatgt tagtagtttc
     3061 aggtatttat gcagtaatca tgtgttggta ttcgtcggtt acgctttgtt ccggcttgag
     3121 aaaaaaatca acgtcaaggt tgttcctgtg ggtttatgga agtggcctga agttgacatt
     3181 cgattggagt actgaaatgt cttcttgagg agttatctga aaaaaataaa aggattaggg
     3241 ttgaatctaa tccttttaaa aaaagatctt atcgatcttg acacacactt aattcacaaa
     3301 agtaatatta ataatcaaat attgtatgaa aaattgaaaa taattttatt ccgaacgttg
     3361 tttttaaata aggatattta tgtcttttaa tacctaatta atcgatttgt tgataataca
     3421 ataataattt cagagtgtat ttatactata attcacgaaa atggagtaat tagtgaataa
     3481 aaaatatttt taaattattt aaataattgg ataaaaaata tactagatag actgcttagg
     3541 tcttcggaaa ataagtactt tatctctttc agatcagcta acgatttata aaaagctttt
     3601 cgatctttaa ctttcaggta gattaacagt tttataaata aatcattggc gtaatagcct
     3661 caaataatta attatttcaa taatcagtat aatcaaatga tagaaaccaa agcttatgca
     3721 gctatgtctt cagattctcc tctggcacca tggagtctgg aaaggcggct tcccggacct
     3781 tatgatgtcc agattgaaat cctgtattgt ggagtatgcc acagtgactt acacacagcc
     3841 agaaatgaat ggcacgatac gaagtacccg gtggttccgg gccatgaaat catcggtaga
     3901 gtagtggcag ttggtattga cgtggtcgac tttcagacgg atgatatagt cggtgtcgga
     3961 tgtatggtgg actcctgtag tcaatgtgaa agctgtaggg agggattgga gcaatattgt
     4021 gaacatggtt cgaccggtac ctacaacagc aaggagagag atggttcagg cgttacatat
     4081 ggcgggtaca gtggcatgat tacggtccgg gaggattttg tcttgcggat tccgggcaac
     4141 ctgcctttaa caggggtagc tcccttgtta tgtgccggaa tcaccactta ctctcccttg
     4201 agacactgga aggtaggaaa gggaatgaag gtgggcattc ttggccttgg cggtcttggc
     4261 catatgggag taaagcttgc tgtagccatg ggcgctgagg taaccatgct tagccatact
     4321 gcaactaagg ctgaagatgc agagcgctta ggggcacatc attttatcct ggcttctgac
     4381 gaaggtcaaa tgaggtctgt acgtagcagt ttcgatttaa ttcttgacac tgtttctgct
     4441 gtacatgact acaactcgta cctgaaacta ctgaagagag atggcacaat ggtttgcctg
     4501 ggcggaccac ctgtcgggcc cggaatagaa tccttcaatc tgataggcaa gcgacgatcc
     4561 attgccgggt ctttgattgg aggtattcgt gagacacagg agatgttgta tttttgtgca
     4621 gaacataata ttgtcagtga tgttgaagtc attcgcatgg attatatcaa tgaggcttac
     4681 gaaagaatgc ttaagggtga tgttcgatat cgttttgtga ttgatatggc aagtctgaag
     4741 gaataacctc gacacctcag tttcttggat tgaccaagaa taggtatgaa gtttatcttt
     4801 tagagattat acctagatga attataaaat aatccgatag ttgtaggtac acttgtcccg
     4861 agagctaagc gaccggagct ctgttctgtc ccggtgtgaa acaaaccgga gtttggggat
     4921 accgtggtta cagtcgtata gttcaatgaa attggtttat tacgaatgaa cattcgatat
     4981 tacttcaatc gttccaagtc tttcataaaa tatacaggct tagggtcaga tacaagctta
     5041 gacgtagatg tcttggatac ccgtacaaaa taataaaagc cgaaaaaatt gatcttatac
     5101 caagatgatt tatataatag tccataaaat ctaggaatac tcgtcccggg agtgaaacaa
     5161 tccggggttc aggtatactt gtcccgagaa ttaagcgacc ggagctctgc tcgatcccgg
     5221 tgtgaaacaa accggagtta gggaatgccg aggttacagt cgtttagtca aattatattg
     5281 gtttattact aatgcatctt cgttattaag gcacatccta ggtgcattca aagcctgtcc
     5341 ttgtcattga ttcactgaaa tcgaagcaag atagatttaa ctaaaaaacc gcaggaagtg
     5401 ctggcttcct gcggaacttc aaaactatca cccgactgaa ttggaagtac gaccgaaaat
     5461 atgaaaatca ttgacttcta tttccgtgac atatttttta ttcccgtttt tatcgatata
     5521 gcttctgttg accagcttgc cgctgacagt tatttctcta cctttggtaa gatatttctc
     5581 ggcaatttca gccggtctgc cccaggcaat cagattgtgc caggtggctg tctcaacgac
     5641 ctttccatcg ttgttggtgt aacg
//
LOCUS       DAOQMC010000157         5643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6334_length_5643_cov_4.759127, whole genome shotgun sequence.
ACCESSION   DAOQMC010000157 DAOQMC010000000
VERSION     DAOQMC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_5643_cov_4.759127"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..614
                     /locus_tag="PKV31_07100"
     CDS             39..614
                     /locus_tag="PKV31_07100"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNB92661.1"
                     /translation="MGKIKSVGIVLAGGLSSRMGTEKSMLLYHGVPFYGVIAKILSIL
                     VDDVHISCRTDQTDNFKDYPVLTDQYPDVGPLGGILTAMEAFPNALLLTVPCDAPTID
                     AELLHNLIRNLTPTANGVFIKNKSDEIEPLIALYHPSSFAQIKNDYRNGILSVQKVIS
                     RLKQPVFISVSDFPANLNTPEDLMKIDTNKP"
     gene            675..2318
                     /locus_tag="PKV31_07105"
     CDS             675..2318
                     /locus_tag="PKV31_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNB92662.1"
                     /translation="MFKRVNFIILLTLLSCSLWSQTNKNTEHEMPKLVVGIVVDQMRY
                     DFLYRYYDRLGENGLKRLIRDGVNCINTHYNYTPTYTGPGHASIYTGSSPAVHGITSN
                     TWYERNLAREVYCTEDNNVKGVGTDSPAGLESPRNMKSTSITDQLKLATGFRSKVIGV
                     ALKDRGSILPAGHSADAAYWYDAETGNFITSTYYRNELPQWALDFNAMQYSEKYLNSV
                     WETLYPLETYVQSDTDNRDYEEPLKGEVLPVFPHKYIPGTKRRYDNIYASPFGSILTT
                     DFALAALKGENLGKNKVTDFLAVSYSSTDYIGHGFGPNSIEVEDAYLRLDIEFEKLFN
                     ALDAQVGKGNYLVFLTADHGICDVPGHSEAHKLPGRILGSKILWDSLNEYLNKTFTPA
                     KYLIKYSSYQYFIDYKQLKNKNISHKEFFDAVRTYLLKHEGIAEVIDLHNIGNTAYPE
                     LFINKIKNGINPKRSGDIFVMLEPGWLGSGKKGTTHGSLYEYDSHVPLIFYGWKLRPR
                     EVVTPVNITDIAPTLAAMLHILAPSGCIGTAIPEIAGSR"
     gene            complement(2474..2740)
                     /gene="secG"
                     /locus_tag="PKV31_07110"
     CDS             complement(2474..2740)
                     /gene="secG"
                     /locus_tag="PKV31_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNB92663.1"
                     /translation="MINFIVVLIAIVGFLLVLVVLIQNPKGGGVDANFGGQAANQIFG
                     ASNSIDFVEKLTWGLASALFVLCIVAAVLVNNSGGGAGPVLLSQ"
     gene            complement(2742..3719)
                     /locus_tag="PKV31_07115"
     CDS             complement(2742..3719)
                     /locus_tag="PKV31_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92664.1"
                     /translation="MLRSIEESVLVKNLLLYPYFGVLYLLQALKRQFKALDQEEEYFP
                     PVLANDGIYSWYRMGVMHSCNQAVDPHDRESEVIGGRICDESHVEDSNESKEEQSEQY
                     VAKRSNGSLIGMVTEVVEVSALDDMEEDTDAGSPEPEDKSRLSHDVETRIIYREKYSL
                     PRITVNSIYEKGGRVSFAGTGDYEYFDPNSEELSGFARFLRKSTRNTSINPEMRSEST
                     PDPSDAKQSHSVAAMSPHLISVKSGEEVEEKSEKKKKKKRKKELVDRLVQQSISDNEL
                     LVSEPFAEILYAQGYKDKAIKIYEKLMLKNPEKRDIFAAKIVLINNEKN"
     gene            complement(4010..4519)
                     /gene="lptE"
                     /locus_tag="PKV31_07120"
     CDS             complement(4010..4519)
                     /gene="lptE"
                     /locus_tag="PKV31_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNB92665.1"
                     /translation="MERSGSSKYLSMILVISLLVSGCYSFRGTSIPSNTNTFYVGNFT
                     LDTYDAPFNLSQMVTEKLKDKIRKETRLKYVEIEPDIEFVGKVTNYVVSPVAPQANQI
                     PSANRLEITINVSFLDQKNEKNKWTSNFTFFKDFSKDANLIDVRDGLANDIFDQLVED
                     VYNRAFTNW"
     gene            complement(4639..>5643)
                     /locus_tag="PKV31_07125"
     CDS             complement(4639..>5643)
                     /locus_tag="PKV31_07125"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNB92666.1"
                     /translation="GRFCYTDVAKEIEKLKQQNVNGIILDLRNNPGGSLNDVVKMAGL
                     FIENGPIVQVKSRDQEPYVMNDDNKSYAYDGPLVILVNHQSASASEIMAAAMQDYKRA
                     VVIGGTSTYGKGTVQRFIDLDRTLSSSSNLRPLGQLKLTIQKYYRIDGGSTQLKGVTP
                     DIILPDALNYIKVGEKENKTAMDWSKINSAQYSQDVFVVRNMDEIKSRSHNRTENNPL
                     FVAIDENAKRIKAQNDQKEFSLELNTYRSELQKRKDDNKKFNGLFKEISGLEVANLPV
                     DMKLIERDSASMARNDNFLKNLRKDVYLDEAINVISDLQSNRITLMDKNSGRVKTGKE
                     "
BASE COUNT         1722 a         1255 c         1014 g         1652 t
ORIGIN      
        1 ttgaatacag aaattacaaa atcattcgcc cctagtccat gggtaaaata aaatcggttg
       61 gaatagtttt ggctggtggg ctcagctcca gaatgggaac agaaaaatct atgttgctat
      121 atcacggtgt cccattttat ggagtaatag ccaaaatcct gagcatattg gtcgatgatg
      181 tccacatttc atgcaggact gatcaaacag ataatttcaa ggactatcct gtacttacag
      241 accaataccc tgacgtagga ccgctaggcg gtattctcac tgctatggag gcatttccaa
      301 acgctttgct tcttactgtt ccctgtgacg cccctaccat cgatgctgaa ttattgcaca
      361 atttaatacg gaatttgact ccgacagcaa atggcgtttt tattaaaaac aaatccgatg
      421 aaatagaacc gttaatcgcc ttgtatcatc cctcttcatt tgcacaaata aaaaatgact
      481 ataggaatgg tattctctcg gtacagaaag taatttcaag gcttaaacaa ccggtcttca
      541 tttcggtaag tgatttcccg gctaatttaa atacaccgga agatttgatg aaaatagata
      601 caaacaaacc ataactgatt tgatccggtt ctataaattt ttgcttagct ttgaattatt
      661 cttaattttc tattatgttc aaaagagtaa atttcataat acttctcacc ctcttatcat
      721 gttcgctttg gagtcagaca aataagaata cggaacatga aatgcctaaa ctggtagttg
      781 gcatcgttgt ggaccagatg agatatgact ttctgtaccg atactatgac aggttgggtg
      841 aaaacggact aaaaagatta atacgagatg gggttaattg catcaataca cattacaatt
      901 atactccgac ctacaccggt cccggacatg cctccatata taccggctcc tctcctgctg
      961 ttcacggtat tacaagcaat acctggtacg aaagaaatct ggcccgggaa gtgtattgta
     1021 ccgaagataa taacgtcaag ggtgtcggaa cggatagccc tgccggattg gaatcaccca
     1081 gaaacatgaa gtcaacatcc atcacagacc aattgaaact ggccaccgga tttcgaagta
     1141 aggttattgg tgttgccctg aaagacagag gttccatctt accggccgga catagtgcag
     1201 atgcggcata ctggtacgat gcagaaaccg gcaattttat aacatcgacg tattacagga
     1261 atgaattgcc tcaatgggcg ttggatttca acgctatgca atattcagag aaatatctga
     1321 attcagtatg ggaaactctg tatccattgg aaacttatgt acaaagcgat acagacaaca
     1381 gagattatga ggaaccgctt aagggagaag tgttgccggt cttcccacac aaatacattc
     1441 cagggacaaa acgcagatac gacaacatct atgcgtcgcc attcggaagc atcttgacaa
     1501 cagacttcgc tcttgcggca ttgaagggag agaatcttgg aaaaaataag gttacagact
     1561 ttctggctgt cagttatagt tccacagatt atattggcca tggcttcggt cccaattcaa
     1621 ttgaagtaga agatgcctac ctgagacttg atatcgaatt cgaaaaatta ttcaatgccc
     1681 tggacgccca ggttggaaaa ggcaactatt tggtattcct caccgctgac catggcattt
     1741 gcgatgtccc aggacatagt gaagctcaca aacttcccgg aaggattctt ggatcaaaaa
     1801 tactttggga cagtctgaat gaatatctga acaagacctt tacacccgcc aaatatctga
     1861 taaaatattc ttcctatcaa tacttcatag attataaaca gctgaagaac aagaatataa
     1921 gccataaaga attctttgat gctgtaagaa cctatctgtt gaaacatgaa ggaatagcag
     1981 aagtgatcga cttgcataac ataggcaaca ctgcctatcc tgaattattc atcaacaaaa
     2041 tcaaaaacgg aatcaacccc aagcgttccg gagatatttt tgtcatgctg gaacctggat
     2101 ggctgggaag tggcaaaaag ggtacaaccc atggctcact ttatgaatat gactcccatg
     2161 tgcctttaat attctacggc tggaagttaa ggcccaggga agtagtgact ccggtcaata
     2221 ttacagacat tgctccaacc cttgctgcca tgctgcatat attggcaccc agcggttgta
     2281 ttggcactgc aataccggaa attgcaggat ccagataaat aaaaatgccg ggttctccgg
     2341 cattttttca actcttttgc tattggttgg aattaaatcc aactatataa aatttgttcg
     2401 gaaacacggg tgatagatac aaataaaggc atgcttatag ctatgccttg aatgtatatt
     2461 tgaaaaaatc aggttattgc gaaagcaaaa caggccctgc accgccacct gaattattca
     2521 ctaagacagc agcaacgata caaagaacga acagcgctga agccagcccc caggtcaact
     2581 tttcaacaaa gtcaattgaa ttggaagcac caaaaatctg gtttgctgcc tgaccgccaa
     2641 aattggcatc cactccccca cctttaggat tctgaatcaa caccaccaat accaacagga
     2701 agccgacgat tgcaatcaag actactataa aattaatcat attaattctt ttcgttattt
     2761 attagcacaa ttttagccgc aaagatatct cttttttccg gatttttcaa cattaatttt
     2821 tcataaattt taatggcctt atccttataa ccctgcgcat ataatatttc agcaaaaggc
     2881 tcagacacca acaattcatt gtccgatata ctttgctgaa caagcctatc gacaagttct
     2941 tttttccttt tcttcttttt cttcttttcg gacttttctt cgacctcctc accactcttc
     3001 actgatatga gatgtggcga catggcagcc acactatggc tttgcttcgc atcggatggg
     3061 tcaggagtcg attctgaacg catctccggg ttaatagaag tatttctggt actcttcctt
     3121 aaaaacctgg cgaaaccgga cagctcctcg ctattcggat caaaatattc gtaatccccg
     3181 gtacctgcaa aggatacccg accacccttt tcataaattg aattgactgt aattcttggc
     3241 aaagagtatt tttctctgta aataatcctt gtctcaacat catgtgacaa ccggctctta
     3301 tcttccggtt ccggagagcc ggcatcggtg tcttcttcca tatcgtccaa tgcagacact
     3361 tcaaccactt cagtaaccat tccgatcaaa ctaccatttg acctctttgc tacatattgc
     3421 tctgattgtt cttctttaga ttcatttgag tcttcaacat ggctttcatc acatatcctg
     3481 ccaccgatta cttccgattc cctatcatga ggatccaccg cttgattgca ggaatgcata
     3541 acgcccattc tataccacga ataaatacca tcattggcta aaacaggagg gaaatattcc
     3601 tcttcctgat ctaaagcttt aaattgcctt tttagggctt gaagtaaata cagaacacca
     3661 aaataagggt ataaaagcaa atttttgacc aatacgcttt cttcaatcga tctaagcatc
     3721 gaaggaaaat gtaacaagcg ctctaccaat tccatataca atttagcatt cagtcaaaaa
     3781 cgctgcaaga taacaaattt gcacaaataa aaatcagaag gaaaggcccg gtaggatcaa
     3841 gcatattcaa tgttaatcac catgtagaca ggatgtcttt catagaaaaa ttaataagat
     3901 catttccgga gagcatagtg ccaacctcgt attgctccaa agtatgaatg acaatctgaa
     3961 tataaaaagg agactaaaac agcttaagcg cccactcaat aatcaatccc taccaattgg
     4021 taaatgcccg attatataca tcctcaacca actgatcgaa gatatcattg gcaagaccgt
     4081 cccgaacatc aatcaaattg gcatctttac taaaatcctt aaaaaaggta aagttggaag
     4141 tccatttatt cttttcattc ttttgatcca gaaagctgac attaattgtg atttcaagcc
     4201 tattggccga tggaatctga ttggcctgag gggcaacagg actcacgaca tagttggtaa
     4261 ccttcccaac aaactcgata tccggctcta tttcaacata tttcaatctg gtctcctttc
     4321 tgattttatc tttcagcttt tcggttacca tctgagatag attgaaaggg gcatcatagg
     4381 tatccagcgt gaaattacca acataaaagg tattcgtatt ggatggaatt gaggttcctc
     4441 gaaatgagta gcaacctgag accaataacg aaattaccag tatcattgag aggtatttgc
     4501 tgcttcctga tcgttccatg aatacaattt tatgcaagta taaaaaacga aaacgaaatc
     4561 tatcctatgg aatcatacaa taaatttcgt tttcatatag gcacattata acctcaagtg
     4621 tgccggcaaa aatagctctt actccttacc tgtttttacc cttccggaat ttttatccat
     4681 caaggttatc ctgttggatt gcaaatcaga aataacattg atagcctcat ccaggtatac
     4741 atcctttctc aggtttttaa gaaaattatc attcctggcc atgcttgcac tatctctctc
     4801 tataagtttc atatctaccg gtagattagc tacttcaaga ccggaaattt ccttgaacag
     4861 gccattgaac tttttgttat cgtcttttct tttctgtaat tcactcctgt atgtattcaa
     4921 ttccagggaa aattcctttt gatcattctg cgccttaatt ctttttgcat tttcatcaat
     4981 tgcaacaaac aatggattat tttcagttct gttgtgagac ctggatttta tctcatccat
     5041 attcctgaca acaaatacat cctggctata ttgggccgaa ttaatcttcg accaatccat
     5101 agctgtctta ttctctttct ctcccacttt aatgtaattc aacgcatccg gcaaaataat
     5161 atcgggtgta acacctttga gttgagtact accaccatcg attctgtaat atttttgaat
     5221 ggtgagtttc aattgaccca atggcctaag gttactgctg cttgaaaggg ttctgtccag
     5281 gtcaataaac ctttgcactg ttccctttcc ataagtagat gttcctccaa taaccactgc
     5341 gcgcttataa tcctgcatcg cagcagccat aatctcagaa gccgatgcac tttgatggtt
     5401 gacgagtatc accaaaggtc catcatacgc ataggattta ttatcatcat tcatgacata
     5461 gggctcctga tctctagatt tgacctgaac gatgggcccg ttttctataa ataacccggc
     5521 catcttaaca acatcattca gagaacctcc cggattattc cttagatcca ggatgatacc
     5581 attgacattt tgctgcttca gcttctctat ttccttagcc acatcggtgt agcagaaccg
     5641 tcc
//
LOCUS       DAOQMC010000158         5502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6637_length_5502_cov_5.442445, whole genome shotgun sequence.
ACCESSION   DAOQMC010000158 DAOQMC010000000
VERSION     DAOQMC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6637_length_5502_cov_5.442445"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..984
                     /locus_tag="PKV31_07130"
     CDS             160..984
                     /locus_tag="PKV31_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNB92667.1"
                     /translation="MWNYIRQSIARKRARRITRKYDTVIDHFEIEGIGRIDFANWTNP
                     LVEKKVISRSQVDFFAKFLKPGDLAVDIGGNVGHMTIAMSLAAGREGRVVSFDPNPFV
                     YDILVKNAGLNPGLTRIDTFPYAIADHEGEFYYNSSEASFNNGGISEDTNSRHGRFAL
                     DHKIKGIKLESLLEEKYPEELGRLRLIKIDTEGYDKEIIRSIRPILQKHKPTVITEVF
                     KKNDASARAEQFELLSSCGYTMYYFSDFVADAEVIRIENKDQMMQWKHFDMYAICN"
     gene            1679..4636
                     /locus_tag="PKV31_07135"
     CDS             1679..4636
                     /locus_tag="PKV31_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNB92668.1"
                     /translation="MKQCLITLFALLSFSFLQAQSVISGSVKDAKGVGIVGATVLVTG
                     SNNGAATDIDGNFRIDNVSGTAVSLTVSSVGYTTQVVKVDFAGRNSASVQVVLVEDYK
                     LLDEVVVVAYGTRKREDLTGSVTSVGEKDFQRGNIATPDQLLTGKVAGLQVTSGGGSA
                     GGGSRLRIRGGASLNASNDPLIVIDGIPVESNGIAGSGNFLNTINPDDIESISVLKDA
                     SATALYGSRASNGVVIVTTKKGSTGKLQWNFNTQLSVSQVAKKVDVYTGDQIRDIIKA
                     TGNQAYINLLGTANTDWQDEIYRSAFGNDNNLSVGGSVANIPFRASVGFNNQDGVLKT
                     NNFKRMSASLNINPSLFDDDLKLNIALKGTQTRNTWADEGAIGAAVSFDPTQPVHAAN
                     KFGDYYEWLQNENTPHPLSTRNPLGLLELRDNTSKVNRFVGNLQADYRLPFLPDLHVL
                     ANVGMDRAFGQGDDIIKALSATNYQTGGRRVHYEQGKTNSLLDLSLFYEKSFGKNSSV
                     DILAGHSYQDFYTDVYNFPSYPEVGDVAIPGTIPTFDSDRPEFRLESYLGRVNVNLAN
                     KYLLTASIRRDASSKFSKDNRVGYFPALAAAWKLKDEFFSGSNTFSELKLRLGWGVTG
                     QQDIGSYYSYLPVYNVSSSTAQYQFGNTFYPFLRPNAYDANIRWETTTTSNVGLDFGF
                     ADSRITGSIDVYKKKTKDLLSVVPVAPGSNFDITLLTNVGNMENKGVEVTVNTIPLKS
                     EKMIWSLGFNVTYNETKITNLLRNQDPSFKGINVSGIGGGTGNTIGKFEVGYAPYTFF
                     VYKQVYDAKTGAPIEGLYEDLNRDGKVDDNDRYLYTKPAPDFMLGLNTQVIIDKLTIG
                     IAGHGAFNNYLYNNFASQGAALRRIEDPLNYISNGSRIYDDTRFVNNQYLSDYFIENA
                     SFFRLDNINIGYNIGRIFRDKASLRLSANIQNVFVISKYRGLDPEIASDSGVDNNIYP
                     RPRIFSIGASLDF"
     gene            4655..>5502
                     /locus_tag="PKV31_07140"
     CDS             4655..>5502
                     /locus_tag="PKV31_07140"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNB92669.1"
                     /translation="MIKMNLKNIIAGLILVLMMGSCANKLDLYPVNDLTPDKAYATAA
                     GYKQVLAKLYGTLTVTGNAGPAGNPDIKGNLDEGSQVGFIRAFFNAQELPTDEAIVAW
                     NDQTIHDFHHLKYTSSDPFILGMYARPIYNITLINEYLRESTTEKVSSRGITGTDAAD
                     IANSRAEARFLRAFNYWVMMDLFGKSTFITEADQVGTALPKEIGRTELYNYIVGELKA
                     IDTELKPARSAEYGRVDQAAAWALLARIYLNAGVYTGTTHYAEAIDYASRVINAGYTL
                     QPDYAR"
BASE COUNT         1545 a         1166 c         1289 g         1502 t
ORIGIN      
        1 attcggggaa ggtaaattct gccggaactc ctttgcatat catcatttta ttttcaatct
       61 ccggttaaga aaaattccgc acgattcaat ttgtcagtat cttggcactt gaattggacg
      121 atgtactcta ttgtccgatt ttcattaaaa aaccggccta tgtggaatta tattagacaa
      181 tccatcgcga gaaagcgcgc ccgtcgtatc acccggaaat acgatacggt aattgatcat
      241 tttgaaattg aaggaatagg gaggatcgac tttgccaact ggaccaatcc tctggtggag
      301 aaaaaagtca tatcccggag tcaggttgat ttctttgcta aatttttaaa gcccggagat
      361 cttgcggtgg atattggagg caatgttggt catatgacga tagcgatgtc gctagctgcc
      421 ggcagggaag gccgggtggt cagttttgac cccaatcctt ttgtttatga cattctggtg
      481 aagaatgccg gattaaatcc cggattgacc cggatcgaca cctttcctta tgccatcgct
      541 gaccatgaag gtgaatttta ttacaattct tccgaagcat ccttcaataa tggcgggata
      601 tccgaagaca cgaacagccg tcacggacgt tttgccctgg atcataagat taagggtatc
      661 aagctggaat cgctgttgga ggaaaaatac ccggaagaac ttggcaggct aagactgatc
      721 aagattgaca ccgaaggtta tgataaggaa atcatccgct ctatccgtcc aattttgcaa
      781 aaacacaaac caacagtaat cacagaggta ttcaaaaaaa acgacgcatc agcaagagcc
      841 gaacaattcg aattgttgtc ctcatgcggt tataccatgt attacttctc cgatttcgta
      901 gcggatgcgg aggtgatcag gatagaaaat aaggatcaga tgatgcaatg gaagcatttc
      961 gatatgtatg ccatatgtaa ttgaccgccg gacaagaggt gccacaaaaa ggcaaacaga
     1021 cgtccgggct cataataata ggagcgttta gccattaccg gtccttaagg cattctgttt
     1081 ttgatgtcgg aaaacttttt tggcatgttg ggcaggcctt ttgctcccgg tacaattatt
     1141 ttttacaaga ttttgtcaaa cttcaattga aggcatgatg ccgcagagcc tcctgatttc
     1201 gtctatggac attgacaaaa tattgaacgg aggcggggaa ttttgaaagt ggacgaaact
     1261 tcctttgttg cctccggagg gggcttatta agggtgatta tacgagggat aagaaagaaa
     1321 catgtttaga ttgactcccg gtcaacctat tttgaattcc cgggattgat gttgaaaatt
     1381 tttcaccacg accgagccct ggttagtgta aacgcgccca taagcaaatt ttattctaaa
     1441 aagtattcga ttaattcgac aaacaaataa ttgtcaataa tttattgctg tatttttcga
     1501 tgttttgata ttataattta tcattttggg aattaacttg ttttttgttt taaaaaggag
     1561 gtcaaaatat tttagttttc agtgaatgcg gcttatcttc gctgaatata atgtgtaaaa
     1621 agtacacatt ataaaatcat tatttgggac aaaaattatt caagcaaaaa attattttat
     1681 gaagcaatgt ttaattacac tgtttgctct gctgtccttc tccttcctcc aagcgcagtc
     1741 cgttatttcc ggcagcgtaa aagacgcaaa gggggttggc attgtcgggg caaccgtttt
     1801 ggtgacgggt tccaacaacg gcgcagcaac tgatatcgat ggcaatttca ggattgacaa
     1861 cgtatccggc accgctgttt ccctcactgt ttcttctgtt ggctatacca cacaagtggt
     1921 gaaggttgat tttgccggcc gaaattcagc atctgttcaa gtggttctgg ttgaagacta
     1981 taagcttctc gatgaagtgg ttgtcgtcgc ctatggtacc agaaaaagag aagatctcac
     2041 cggatcagtt acttctgtgg gagagaagga ttttcagcgt ggaaacatag cgactccgga
     2101 tcagctactg accggaaagg ttgcgggact tcaggttacc agtggtggag gttctgccgg
     2161 gggtggctcc aggttgcgta tccgtggtgg agcatcactc aatgccagca acgatcctct
     2221 tatcgttatc gatggtattc cggttgaaag caatggtatt gcgggttcgg gcaactttct
     2281 caacaccatt aatccggatg atatcgaatc catctctgtg ctgaaagatg cctccgcgac
     2341 tgcattatac ggttcacgtg cttccaatgg cgtagtgata gtaactacca aaaagggtag
     2401 tacaggaaag ttacaatgga acttcaacac gcaactttct gtctcacaag tcgccaagaa
     2461 agtggatgtc tataccggtg atcagatacg cgatatcatc aaggctaccg gtaatcaagc
     2521 ctacatcaac ctgcttggta ccgcaaatac agactggcag gatgaaatct atcgttcagc
     2581 attcggcaat gacaataatc tcagtgtggg cggatcagta gcaaatattc ctttccgcgc
     2641 ctcggtcggc ttcaacaatc aggatggtgt tttaaagacc aacaacttca aaaggatgtc
     2701 tgccagtctg aatatcaatc cttcattatt tgatgatgac cttaagttga atattgcact
     2761 gaaagggact cagacacgca acacatgggc tgatgagggg gcaattggtg ctgctgtttc
     2821 gtttgacccg actcaaccgg tacatgccgc caataaattt ggcgactatt atgagtggct
     2881 acagaacgag aatacgcctc accccctttc cacacgtaat ccgttgggcc ttctggagct
     2941 cagggataac acctctaagg tcaaccgatt tgtcggaaat ttgcaggcgg actatagatt
     3001 gccattcttg ccggatcttc atgttcttgc caatgtgggc atggataggg cattcggtca
     3061 gggagacgat attatcaaag ctttgtcggc aaccaactat cagacagggg gtcgaagagt
     3121 tcactatgag cagggcaaga ccaattcctt gctggacctg tcattattct atgaaaaatc
     3181 ctttggaaag aattcttccg tggatatctt ggccggacac agttatcagg acttctatac
     3241 cgatgtatac aacttcccat cctatcctga agttggagac gtggccattc ccggcacgat
     3301 acccactttt gatagtgatc gccctgaatt caggctggaa tcttatttgg gtcgtgtcaa
     3361 cgtgaatctc gccaacaagt atctgttgac agcttctatc cgtcgtgacg cgtcttccaa
     3421 attctcaaaa gacaatcgcg ttggctattt cccggcattg gcagcagcct ggaagctcaa
     3481 agatgagttt ttcagcggct ccaatacttt ttccgaactt aaactgagac tcggatgggg
     3541 tgtgacaggg cagcaagaca taggatcgta ttattcctat ctgccggtgt ataatgtgag
     3601 tagttcaact gctcaatatc agtttggcaa tacgttctat cctttccttc gccctaatgc
     3661 atacgatgcc aatatccgat gggagaccac gacaacaagc aatgttggat tggactttgg
     3721 ttttgcggat agcagaatca caggtagcat tgatgtctac aagaaaaaga ccaaggatct
     3781 gctgagtgtg gtaccggtag ctccaggctc taattttgat attacgctcc tcaccaatgt
     3841 gggcaacatg gaaaacaaag gcgttgaagt tacagtgaac accattccac tgaagagtga
     3901 aaaaatgata tggagcctcg gtttcaatgt tacatataac gaaacaaaga ttaccaacct
     3961 gcttagaaat caagacccct ctttcaaggg tatcaatgtt tccggaatcg gtggcggtac
     4021 aggaaataca attggaaagt ttgaagtggg gtacgctcca tatactttct tcgtctataa
     4081 gcaggtatat gatgccaaga ccggagctcc gatcgaaggc ttgtacgagg atctgaatcg
     4141 agacgggaag gttgatgaca acgatcgtta cctgtacact aagccggcgc cggactttat
     4201 gcttggtctg aacacgcagg tcatcattga caaattgacc attggtattg caggacatgg
     4261 tgcattcaac aactatctgt acaacaactt tgcttctcag ggagcagcat tgagaaggat
     4321 tgaggacccg ctgaactaca tttccaacgg atcacgtatc tatgatgata ccagatttgt
     4381 caacaatcag tatttgtccg attattttat tgaaaatgcc tctttcttca gattagacaa
     4441 tatcaatatc ggatataaca taggccgcat attcagagac aaggcatcct tgagattgtc
     4501 cgccaatatt cagaatgtat tcgtcatttc caagtaccgg ggccttgatc ccgagattgc
     4561 aagtgattca ggtgttgata acaatattta tccgcgtccg agaattttct caattggggc
     4621 aagcttagat ttctaaattt taaatgaaca agaaatgatt aaaatgaatt tgaaaaatat
     4681 aatagccgga ttgatactgg tgttgatgat gggctcttgt gccaacaaac tggatctgta
     4741 tccggtcaac gatctgacac cggacaaggc atatgccacc gccgcggggt acaaacaggt
     4801 actggccaag ctttatggca cgctgactgt gacaggcaat gctggtccgg cagggaaccc
     4861 ggacatcaaa ggcaatctgg atgaaggatc acaggtaggt tttatccgtg ccttcttcaa
     4921 tgcgcaggaa ttgcctaccg atgaagctat tgtcgcctgg aatgaccaga ctatccacga
     4981 cttccaccac ttgaagtaca cttcttcaga cccctttatc ctgggtatgt atgcgaggcc
     5041 gatatataac attacgctca tcaatgaata cctccgcgaa tcgacaactg aaaaggtctc
     5101 atcaagaggc atcacgggta ctgatgcggc agatatcgcc aacagtcgtg cggaagcgcg
     5161 gttccttaga gcatttaact attgggtgat gatggacttg tttggcaagt caactttcat
     5221 tactgaagca gatcaggttg gaacagctct tccaaaggag attggaagaa cagaactgta
     5281 caactacatc gttggtgagt tgaaagcgat cgatactgaa ctgaagccag ccaggtctgc
     5341 tgaatatggc cgggttgatc aggcagcagc atgggcattg ctggcgcgta tttacctgaa
     5401 tgccggtgtg tatacaggta caacgcacta tgccgaagca atagactacg cttccagggt
     5461 aatcaatgcc ggatatactc tgcagccgga ctatgcaaga tt
//
LOCUS       DAOQMC010000159         5466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6708_length_5466_cov_6.226021, whole genome shotgun sequence.
ACCESSION   DAOQMC010000159 DAOQMC010000000
VERSION     DAOQMC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6708_length_5466_cov_6.226021"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /gene="secA"
                     /locus_tag="PKV31_07145"
     CDS             complement(<1..1833)
                     /gene="secA"
                     /locus_tag="PKV31_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692249.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNB92670.1"
                     /translation="MLDFIKKLFGTKTEREVKAYMPIIDEINGYFESYKSLSNDELRN
                     KTTEFRERIGNHLAGIDSDIKSLNDQARNTQNIREKESIFDQVDKMKKDRDKHLEDIL
                     RQILPEAFAVVKETARRLANNDELEVTATQHDMDLAATKPYVRVENGKAFWKNQWTAG
                     GNEIKWDMVHYDVQLIGGMVLHDGKISEMATGEGKTLVASLPAYLNGLSGQGVHIVTV
                     NDYLAKRDCQWNGPLYQFLLLTVDCIDNYRPNSAERRAAYLCDITYGTNNEFGFDYLR
                     DNMVTSPDELVQRKHHYAMVDEVDSVLIDDARTPLIISGPIPQGSDEQEYIDLKPRVE
                     RLVDAQRKLATGFLVEAKKLISENISGNAEGEGGLALLRAYRALPKTRALIKYLSEEG
                     VKVVLQKTENYYMQEQSKNMHIADEPLLFTIDEKNRDVELTEKGAEYLAQGMEDKDFF
                     VLPDIGTEIHSLEHDSSLTAAQKAEEKEKLIQDYTAKSKRLHSINQLLKAYALFEKDV
                     DYIIVEGEVKIVDEQTGRVMEGRRYSDGLHQALEAKENVKVGEQTQTYATVTLQNYFR
                     MYHKLAGMTGTAETEASEFWQIYKLDVSVIPTNKPIVRKDMEDLV"
     gene            2238..2936
                     /locus_tag="PKV31_07150"
     CDS             2238..2936
                     /locus_tag="PKV31_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92671.1"
                     /translation="MKLSLGTSIYLLKLFFALSTIILFSCQSTEVDTPLKSYYFPLSQ
                     LTQGKKYTYVCGPNDSLDDQIFTLISQKKSGKMFLSGSISRSDGVLTHQWQEEETRTG
                     MVMTEYAMFLSKDKPQKTQIVYDDIFPFKADPGGIFLFDMKWRDPASPDASYQLIRNR
                     IYVGDTTMQIMGKEVKCVHFKVRERLEVDQEGILGLDMNGDEYYGQGIGLVRFVRQVN
                     DRKPVYTLSSINDH"
     gene            3828..4424
                     /locus_tag="PKV31_07155"
     CDS             3828..4424
                     /locus_tag="PKV31_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNB92672.1"
                     /translation="MVEDLLVSDDVVKEHFACDLNACKGACCWEGDFGAPLDKKELKI
                     LDDIKDKVSSYLSEASNKVLAKHGAYTYFEEMEDYGTTLMPDGACVFMTTNELGIAGC
                     GIEQAYRDGVVDWPKPESCHLYPIRVTKNAEGNDYALNYDRWSICRNACSKKDAPQIR
                     LFEFVKDGLIRRYGKEFYEQLDYLAGVVNGPDVPESNG"
     gene            4424..>5466
                     /gene="hemW"
                     /locus_tag="PKV31_07160"
     CDS             4424..>5466
                     /gene="hemW"
                     /locus_tag="PKV31_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNB92673.1"
                     /translation="MSFSGGLYIHIPFCRQACHYCNFHFTTSLHYKSRLVEAINREIR
                     IRAAHFEKPELKTIYFGGGSPSLLSISELNTIMESIHRVFNTSGVVEITLEANPDDLT
                     PEYIRLLKTIGIDRLSIGIQSFYEEDLMWMHRAHNNIQAMNCIPMAQDIGLGDISVDL
                     IFGFPLLTDEKYMENLKKLTDWNIPHISAYSLTVEDKTPLAKSIRKGIDSRPDEGKSA
                     RQFVLTHHILEGKGYEHYEISNFSLPGWHAIHNSNYWLGVPYIGVGPAAHSFYEDCRY
                     WNIANNQKYMHLIESGQETGEYEIINPDTAYNELILTRLRTKWGVDRADILRIGTKYA
                     DHFDSIIGVLIDNG"
BASE COUNT         1575 a         1188 c         1091 g         1612 t
ORIGIN      
        1 caccagatcc tccatgtcct ttcttacaat aggcttattg gtcggaatga ctgaaacatc
       61 gagtttgtat atctgccaaa attcacttgc ttcggtctcg gcagtacccg tcatacctgc
      121 cagcttatga tacattctga agtaattctg taatgttact gtagcatagg tttgtgtctg
      181 ttctcccacc ttgacgttct ccttggcttc cagtgcctga tgaagcccat ctgaataacg
      241 acgtccttcc ataacacgcc ctgtctgctc atcgactatc tttacctcgc cttccactat
      301 gatataatct acgtctttct cgaacaatgc atatgccttc aaaagctggt taatagagtg
      361 aagacgtttg gatttggcgg tataatcctg tatcaatttc tccttttctt cggctttctg
      421 agcagcagtc aacgatgaat cgtgctcaag ggagtgaatt tcggttccaa tatcaggaag
      481 tacaaagaaa tccttatctt ccatgccctg agcaagatat tcagcaccct tttcagtcaa
      541 ttcgacatcg cgattctttt cgtcaatcgt aaaaagcaaa ggctcatctg caatatgcat
      601 attcttggat tgttcctgca tataataatt ttctgtcttt tgcaagacaa ccttcacccc
      661 ttcttcactc aaatacttga taagcgctct tgtctttgga agtgccctat aagcgcgtaa
      721 taatgccaag ccaccttcac cctcagcatt accagaaata ttttcactaa ttaatttctt
      781 cgcctctaca aggaagcctg ttgcaagttt cctctgagca tccaccaaac gctccacacg
      841 tggtttcagg tcgatatatt cctgttcgtc cgaaccttgt gggattggcc cggaaatgat
      901 tagcggcgtc cttgcatcat cgatcaatac ggagtcaact tcgtcaacca tggcgtaatg
      961 gtgcttacgt tgcaccaatt cgtcaggact tgtaaccatg ttatcacgca gatagtcaaa
     1021 accaaattcg ttgttggttc cataagtgat gtcacacagg taggcagctc gtctttctgc
     1081 ggaattggga cggtagttat cgatacaatc cactgtcaac agaaggaatt gatatagagg
     1141 accattccac tggcagtccc tttttgccag ataatcgttg actgtcacga tatgaacacc
     1201 ttgaccggac agtccgttga ggtaagccgg taatgaagcg acgagagtct ttccttcccc
     1261 tgtcgccatt tctgagatct ttccatcgtg cagcaccata ccaccaatta actgtacatc
     1321 atagtgcacc atatcccatt tgatctcatt tcctcctgca gtccactgat ttttccaaaa
     1381 agctttgcca ttctccacac gtacataagg cttggtagct gcaaggtcca tgtcatgctg
     1441 agtggcagtt acttccaact catcgttatt ggcaagtctg cgtgctgtct ccttaaccac
     1501 ggcaaaggcc tcaggtaata tctgtctaag aatgtcctcc agatgtttat cccgatcttt
     1561 cttcatctta tccacctgat cgaagatgga ttccttttca cggatattct gtgtatttct
     1621 cgcctggtca ttcagacttt tgatatcaga atcgattccg gcaaggtgat tacctattcg
     1681 ttcacggaat tcggttgttt tattccttaa ttcatcatta gaaagtgatt tgtaggattc
     1741 aaagtatcca ttgatttcat cgatgatggg catgtatgcc ttaacttctc tttcggtttt
     1801 agtaccgaac aactttttta taaaatccag cattataaaa tttttatgtc attgagttga
     1861 ctaaattcgc tttgaatttg ggatttaaat aaatccttct ttaaaataaa gttgcaaagg
     1921 tacgttttat agtcaaacac aatttttatt atcaattaat tttgcaatga ttaacaccga
     1981 ttatttcctc aaaatattat cattaaaggc taagggtatg gacacatcgt tttccattac
     2041 attaatcaca tttacctatt tgatctttcc aaaaaactgc tacggattca agccggcaac
     2101 atagatatcc ttaaacaaag ctgtcagaaa catcacattt catgaaacaa atttattgcc
     2161 aattggttac gactacaaaa aacatgtcaa aatgtctgaa tgacacccca atattgacaa
     2221 aattcaaatc taaagaaatg aaattatcct tgggcacctc tatttatctg cttaaattgt
     2281 tttttgcgct atcaactatt attttatttt cgtgtcagtc cactgaagtc gatacaccct
     2341 tgaagtccta ttatttccca ttgagtcaat taacccaagg caagaaatat acctacgtct
     2401 gcggtcctaa tgattccctt gacgatcaga tttttacgct catttcccaa aaaaaatccg
     2461 gaaaaatgtt tctcagtgga agcataagtc gtagcgatgg tgtgttgacc catcaatggc
     2521 aggaggaaga gaccagaaca ggaatggtta tgacagaata cgctatgttt ttgtccaagg
     2581 ataaaccaca gaagactcag attgtgtacg atgatatatt tccgtttaaa gccgatccgg
     2641 gcgggatatt cctgttcgat atgaaatgga gggaccccgc cagtcccgac gcttcctatc
     2701 agctgatacg gaaccggatt tatgtcggag acactacgat gcaaatcatg ggcaaggaag
     2761 taaaatgtgt acattttaag gtgagggagc ggctggaggt ggatcaggaa ggtatattgg
     2821 gacttgacat gaatggtgac gagtattatg gtcaaggtat cggattggta cgattcgtac
     2881 gacaggtgaa tgataggaag ccggtctata cgctttcgtc aataaatgat cactgaatgg
     2941 ggttatgaga aagatatcat tacaaaatca agaggaaggg ttggggcgga gagcaggcac
     3001 actgaagtag gtagatgatt aataacctac atcctgattg gacaggcgat cggaaacgcc
     3061 cactcatatt ttctgcatta aatcgaaact tttctggtcc ttcattttta tatcgtacag
     3121 agccagtgca gattttcggc tattactttc gtacctactt caattttgaa actccgccaa
     3181 atgccaaaat tcagtgaaag ttcaaaaaaa ggaggttcgg agagtagcca ctctgaagta
     3241 ggtagatgat taataaccta catcctgatt ggacaggcga tcggaaacgc ccactcatat
     3301 tttctgcatt aaatcgaaac ttttctggtc cttcattttt atatcacaca gagccagtgc
     3361 agattttcgg ctattacttt cgtacctact tcgattttga aactctgcca aatgccaaaa
     3421 ttcagtgaaa gttcaaaaaa aggaggttcg gagagcaggc acactgaagt aggtagatga
     3481 ttaataacct acatcctgat tggacaggcg atcggaaacg cccactcata ttttctgcat
     3541 taaatcgaaa cttttctggt ccttcatttt tatatcgtac agagccagtg cagattttcg
     3601 gctattactt tcgtacctac ttcgatttta aaactccgcc aactcatgca cccctcactt
     3661 tttcaccata cccttcaatc cctgtataga gatagctgat tatacaacca gccaacatat
     3721 aaaacactga ttcaaatatt tcaaagtaca gttccttcct tttttctttt cagcacgcaa
     3781 cacaattatc attacctttg tcgaaatatc ctgattgaat tatgttaatg gtggaagacc
     3841 tactggtgag cgatgatgta gtcaaggagc attttgcatg cgaccttaat gcatgcaagg
     3901 gagcttgctg ctgggaaggt gactttggag cgcctttgga caaaaaagaa cttaagatat
     3961 tggacgatat taaggacaaa gtatcctctt acctttcaga agcgtccaat aaagtgttag
     4021 ctaaacatgg ggcttatacc tattttgaag aaatggagga ttatggtacg accctaatgc
     4081 ctgacggagc atgtgtcttt atgacaacga atgaactagg catcgccggg tgcggtatcg
     4141 aacaagctta tcgcgatggt gtggtagatt ggcccaaacc ggaaagctgc cacttatatc
     4201 ccatccgggt gacgaaaaat gctgaaggta acgactatgc actcaactat gatcgatggt
     4261 ccatatgtcg aaacgcatgc agcaaaaaag atgccccaca gataaggtta tttgagtttg
     4321 tcaaggatgg tttgatacga agatacggaa aggagtttta tgaacagttg gattatctgg
     4381 caggcgtagt caacgggccg gatgttcctg aaagtaatgg ttaatgtctt tttccggagg
     4441 gctatacatt catattccat tttgcaggca agcatgtcat tattgtaatt ttcattttac
     4501 cacttccctt cattacaagt cccgactggt ggaagcaatc aatagggaaa taaggatacg
     4561 ggcagctcac tttgaaaaac cggaattgaa aaccatctac tttggcggtg gcagtccatc
     4621 cctactcagt atttcagagc tcaacacaat tatggaatcc attcatcgcg tattcaatac
     4681 ctcaggtgta gtggaaatca cattggaagc caatccggac gatcttaccc ctgaatatat
     4741 ccgtctatta aaaacgattg gcatcgatcg cctgagtatt ggcatccaat ccttttatga
     4801 agaagacttg atgtggatgc accgtgccca caataatatt caggcaatga attgcatacc
     4861 tatggctcag gatatcggcc tgggggatat ttctgttgac ctcatctttg gctttccgtt
     4921 gcttacggat gaaaaatata tggaaaatct taaaaaacta accgattgga acattcctca
     4981 catttcggct tacagtctaa cggtagagga taaaacaccc ctggccaaaa gcattcgaaa
     5041 aggtatagat tcaagaccgg acgaaggcaa atctgctcga cagtttgtac tcacgcatca
     5101 tatcttggaa ggtaaaggtt atgaacacta cgaaatatcc aatttttcac tacccggatg
     5161 gcatgcgatt cacaacagca actattggct cggtgtgccc tatataggtg ttgggccggc
     5221 agctcattcc ttctatgaag actgccgata ttggaatatc gccaataacc aaaaatatat
     5281 gcacctgata gaatccggtc aggaaaccgg tgaatatgaa ataataaacc cggataccgc
     5341 atacaatgaa ttgatcctga cacgcctccg aacaaaatgg ggtgtcgatc gtgcagacat
     5401 cctgcgaatt ggcacaaaat atgcagacca ttttgacagt atcattgggg tgctgattga
     5461 caatgg
//
LOCUS       DAOQMC010000160         5445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6754_length_5445_cov_4.812245, whole genome shotgun sequence.
ACCESSION   DAOQMC010000160 DAOQMC010000000
VERSION     DAOQMC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6754_length_5445_cov_4.812245"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="tsaB"
                     /locus_tag="PKV31_07165"
     CDS             complement(<1..681)
                     /gene="tsaB"
                     /locus_tag="PKV31_07165"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNB92674.1"
                     /translation="MKTFLAIDTISVPFSIAIGNQESVLAEEILFDRMALAGQITLMV
                     DRCCKKAGLDLSSLSGILISKGPGSYTGLRIGYSIAKGLCMAMSLPLIEVDPMMAMAF
                     VSMEQTGDALNPVFCSALDARRNEVYLAIYNKYGREILKPQPCILPIELKDLIGDIRP
                     DLICCSGDGALKLEGQLGDWNYRTTGICSIASYLFKVMDAGVEHPDFDSIASAEPLYL
                     KPPNITISK"
     gene            complement(683..2320)
                     /locus_tag="PKV31_07170"
     CDS             complement(683..2320)
                     /locus_tag="PKV31_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB92675.1"
                     /translation="MDSYKGVKTHFFSGLVILWIFLLCLTLGAPLLSAQEIASTDLKL
                     FHYSHFINRQELRTHVGLLASDSLEGRGLGTFGSRKASEYIAGQLKNEGLYPIGDRET
                     YFQPVVFKQWYRDKAEFLLTLPDGKIRGQLMPGRDFTFNLNELPMPYSLSADHLMFAG
                     YGIADDAYNDFKYAQAWDEVLLILDGEPMEKGKYVISNTIQPSSWSLGLDRKLQTAVH
                     KGAKAVILIEDSTTYVKRGASEYPGRGKFAEQIVYNYPIPVIRVSEAALVRLFAPKEH
                     ARLAGMIARVKRGDVASNYVQGGFKIDLSITHTLVRDRNVVGLIEGDDPRLKHEYIVL
                     SAHFDHLGIINGEIFNGADDNATGTAALISISKALKALKDNGYPLKRSVLFLFCTGEE
                     SGLLGSRYFVNNPLVPLKHIKADINIDMIGRRDTLHAEKENYLYVIGSDKINPLLDDI
                     LNESNDKTIRYGLDYTYNNVDHPLRLYYRSDHYNFAEKGIPSVFLFGGFHKDYHRPSD
                     DTMLIDFKKVEDISKLVFFTTVGLSNYSGEIKTDYKP"
     gene            complement(2629..>5445)
                     /locus_tag="PKV31_07175"
     CDS             complement(2629..>5445)
                     /locus_tag="PKV31_07175"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNB92676.1"
                     /translation="LMSGLLTAFAGMFICWTTMYLGKLSLVGRGNESDSGQNIGLLLA
                     GLVAGLTSTFCISVWFSAMEGEVYAMSTFFTALTLWSLLKWYYLPDTQHSDRWIVFSL
                     FMAGLSIGVHLLSILTFPALAIFYYLKKFNKFTWTGLLISIVAGLVVLFIVQRVIIVG
                     LPDLLVSLDIFAVNSLGLPFYSGLVPFFAIIGGLLYWGIKKANSRGMHNLQLGLVSLM
                     LIIVAYSTTTVAVIRANANPPINMNNPSDPARLVPYLNREQYGDRPLLFGPSFDAKPI
                     GLKSEPRYGRVGDKYEVIDMKYEYEYKPEDNMFFPRMGHGDDPTKVRLYKQWMGHDGK
                     PTMGDNLSFFWRYQLGWMYWRYFMWNFVGKQNGDQGYVPWDKSKGNWLSGISFIDNTR
                     LGNQDQLPSFEKNNQARNKYYFLPFILGIIGTIFHLKRRRNDWIGLFMLFIITGIGII
                     VYSNQPPIEPRERDYVLVGSFFTFAIWVGLGGLAIGEFVARKIKHMSLPGYAVAGLIA
                     LLAPTVMAFQNYDDMGRRGIYASRDYAANFLNSVGRDAIIFTYGDNDTYPLWYAQEVE
                     GIRPDVRVVNLSLIAVDWYIEGQRRKINESPYIKMTIPSDSYRGNKRNQVYYVDTELA
                     NQELPAEGVLRFIGESHPLEGSGSKAESFLPTKNVYIPIDKAKWINKKFLSPQDSILD
                     RLPLSLGVKEQGDYILKGDLAVMDIIASNINDRPIYFSVTADPSKFLGLNNFMQLEGL
                     ALRIVPVRSNSDRQFGVFGTGRVQTDTLYNNVMKKFRWGNFDKKKLFVDRSYTPTVNA
                     LRMIQMRAAFDMISKGDKKRAADLAENTLKVFPNMNFPFDGAIIPALSMLTTADNIDR
                     AKPYMKQLAENLREKMVYFHSMTPVELSEGYQADKEETESAIADLMDMIGRSKDQGFE
                     EEIRKIIGPYIPKADGAIPKMDER"
BASE COUNT         1497 a         1321 c         1113 g         1514 t
ORIGIN      
        1 cttggatatg gtaatgttag gtggtttgag gtaaagcggc tctgcgctcg ctatagagtc
       61 aaaatcggga tgttccactc ctgcatccat gaccttaaac aggtaggacg ctatactgca
      121 gattcctgtt gtacgatagt tccaatctcc caactgacct tccagtttta atgctccatc
      181 gccgctgcaa caaatcaaat caggacgaat atccccaatc agatctttga gctctatcgg
      241 aaggatacat ggctgaggtt tcagaatttc tcttccatat ttattatata tagcaagata
      301 tacctcattg cgcctggcat ccagagctga acaaaaaaca gggttcaatg catctcctgt
      361 ttgctccatt gatacaaatg ccattgccat catcgggtcg acctcaatca gaggaagact
      421 catcgccata cataatcctt ttgctatgga atatcctatt ctcagaccgg tataactacc
      481 ggggcctttt gaaatcaaaa tgcccgacaa gctgctcagg tccaggccgg cttttttaca
      541 gcaacggtca accataagtg taatctgccc tgccagggcc atacgatcaa acagaatctc
      601 ctcggccaat acgctttcct gattgccgat cgctattgaa aaaggaaccg agatggtatc
      661 tatagcaaga aaagtcttca tatcagggct tgtaatcagt tttgatctct ccggaatagt
      721 tggataatcc aaccgtcgta aaaaacacga gtttgctgat atcttctacc tttttaaaat
      781 ctatcaacat ggtatcgtct gaaggccgat ggtaatcctt atggaagcct ccgaataaaa
      841 acacacttgg aatacctttt tcggcgaaat tataatggtc gcttctgtaa taaagcctca
      901 aggggtgatc gacattgttg taagtataat ccagcccgta tctgatggtt ttatcgttac
      961 tttcattcaa tatatcatca agcaaaggat taatcttatc cgacccaatc acatataaat
     1021 agttttcctt ttctgcatga agcgtatccc gacgaccgat catgtctatg ttgatgtctg
     1081 cctttatgtg tttgagaggc accaggggat tgtttacaaa ataacgggaa cccaacagcc
     1141 cggattcctc tccggtacaa aagagaaaca agacgcttct cttcaacgga tatccgttgt
     1201 ccttcaatgc ttttaacgct tttgaaatag agatcagcgc tgcggtaccg gtggcattat
     1261 catctgcgcc attgaagatt tcaccgttta taatacccaa atgatcaaag tgggctgaca
     1321 gtacaatgta ttcgtgtttc agcctgggat catcaccttc tatgagtccc accacatttc
     1381 gatcccggac taatgtgtgc gttatcgaca gatcaatttt aaatccgccc tgaacgtaat
     1441 tggaagccac atcaccacgc ttgactctgg ctatcatccc tgcaagcctt gcgtgttctt
     1501 ttggggcaaa taatctcacc aatgcggctt cactcactct gatgaccggt atcggataat
     1561 tatatactat ttgctcggca aattttcctc ttcccggata ttccgacgct ccccgcttga
     1621 cataagtagt ggagtcctct atcagaataa ccgcttttgc ccctttgtgt actgctgtct
     1681 gcaatttcct gtccagccca agactccatg aggagggctg tatggtgttg gatatcacgt
     1741 acttgccttt ttccatgggc tcaccatcca ggattaatag aacctcatcc caggcctgag
     1801 catatttaaa gtcattgtat gcatcgtcag caatgccata tccggcaaac atcaaatgat
     1861 ctgcactcaa tgaataaggc ataggtagtt cgttgaggtt gaatgtaaaa tcacgcccgg
     1921 gcataagctg accccttatt tttccatcag ggagtgtaag tagaaattcc gccttgtccc
     1981 tgtaccattg cttgaaaact acaggctgaa aatatgtctc cctgtctcca atcgggtaga
     2041 ggccttcatt tttcaattga ccggctatat attcactggc cttgcgacta ccgaaggtcc
     2101 ccagtccccg cccttccagc gaatcggagg caagcaatcc gacatgagtc ctaagctcct
     2161 gacgattgat gaagtgtgaa tagtgaaaga gtttgaggtc cgttgaggca atttcctgtg
     2221 ctgacaacaa tggagctccc agagtaaggc acaatagaaa tatccataaa ataacaagtc
     2281 ctgaaaaaaa atgtgtcttt acgcctttgt atgaatccat attaattgtt tctcccttcg
     2341 attaaatcct gtccatttta ttttgttttg gccgggtcag tacaaactct gtatttactg
     2401 tctcaaagga ataataaatg agaatacttt tttacctggt cgggaaagcg gtctcaatcg
     2461 attgacctga tccctcccgg atcccttact aatagtttat tcaaattctg atcattacga
     2521 cctaaacttc gttaactctt tatggtacaa tgccggaaac atggcgctac cacatttccg
     2581 gcattatgtc cgggcaaaga tcagcaggcg aatggtgcca cccggtcctt atcgttcgtc
     2641 catcttgggt atagccccat ctgccttagg aatatatggg cctatgatct tcctgatttc
     2701 ctcttcaaat ccctggtctt tggatcggcc gatcatatcc atcaaatccg caatagcact
     2761 ttctgtttct tctttgtccg cctgatatcc ttccgacaac tccaccggag tcatactgtg
     2821 gaagtacacc attttttctc ggaggttttc agccaattgc ttcatgtatg gctttgcccg
     2881 gtcgatatta tctgcggtag tcagcatgga caaggccggt atgatggctc cgtcaaatgg
     2941 aaagttcatg ttggggaata ccttcagtgt gttttctgcc aggtctgccg cccgcttctt
     3001 atcccctttg ctgatcatat caaatgcagc cctcatctgt atcatgcgca aagcattaac
     3061 tgtaggcgta taagaacgat cgacaaacaa tttcttctta tcaaaattgc cccagcggaa
     3121 ctttttcatc acattattat atagtgtgtc tgtttgcacc ctgccggttc caaatacgcc
     3181 gaattgtctg tctgaattgg atctgacagg tacaattcgc agtgcgagtc cttcaagctg
     3241 catgaagtta tttaagccga ggaacttgga aggatctgct gtgacagaga aataaatcgg
     3301 tctgtcattg atattactcg caatgatatc catcacggcc aggtcgcctt tcaatatata
     3361 gtctccttgt tccttgacgc ccaatgacaa gggcagtctg tccaggatag aatcctgagg
     3421 gctcaggaac tttttattaa tccacttggc tttatcaata gggatgtata cgtttttggt
     3481 gggcaggaag gattcagcct tggatcctga gccttccagc ggatgagact ccccaataaa
     3541 tctgagtacc ccttctgccg gcaattcctg attggcaagc tctgtatcca cataatacac
     3601 ctgattgcgc ttgttacctc tgtatgagtc agatggaatc gtcattttga tataaggtga
     3661 ttcattgatc ttccttcttt gcccctcgat ataccaatcc acagcaatca gagacaggtt
     3721 gacaacccgt acatccggac ggattccttc cacctcctgt gcataccaca acggataggt
     3781 gtcattatca ccataggtaa atatgatggc atccctgccc accgaattca ggaaattggc
     3841 tgcataatct cgtgaagcgt agatgccgcg tcggcccata tcgtcatagt tctggaaggc
     3901 catcactgtt ggagccaata gagcaatcaa acccgccaca gcatatcccg ggaggctcat
     3961 atgtttgatt tttctggcta caaattcacc tatagcgaga cctccaagcc ccacccaaat
     4021 agcgaaggta aagaaggaac ctacaaggac ataatccctt tctctcggct caataggtgg
     4081 ttgattggaa tacacaataa ttccaatacc ggttatgata aatagcatga ataaacctat
     4141 ccaatcattc ctcctcctct ttaaatggaa gatggttcca atgatgccca gaataaatgg
     4201 caggaagtaa tatttgtttc ttgcctgatt gttcttttca aaggaaggga gctgatcctg
     4261 attgcccaac cgcgtattgt cgatgaatga aataccgctc aaccagttgc ccttggactt
     4321 atcccatggc acataaccct gatctccatt ctgcttgccg acaaagttcc acatgaaata
     4381 tcgccagtac atccatccca gctgatagcg ccagaagaag gaaagattgt cgcccatagt
     4441 aggcttgcca tcatgcccca tccattgctt gtagagtcta accttggtcg gatcatcacc
     4501 atgtcccata cgtgggaaga acatattatc ctcaggcttg tactcatatt catatttcat
     4561 atcgatcact tcatatttgt cacctacacg cccataacgc ggctcactct tgagtccgat
     4621 gggcttggca tcaaaggaag gtccaaaaag caacggcctg tcgccatact gctctctgtt
     4681 aaggtatgga acgagccggg cagggtctga aggattgttc atattgatgg gtgggttggc
     4741 attggccctt atcacagcca ctgttgtagt tgaataggca actataatga gcatcaagct
     4801 gacaagaccc agctgaagat tgtgcatacc acgtgaattg gcctttttta tcccccaata
     4861 aaggagaccg ccaatgatgg caaagaacgg tacaagtcct gaatagaaag gcaaacccaa
     4921 tgagttgact gcaaatatgt ccagtgaaac caacaagtcg ggaagaccta caatgataac
     4981 gcgttgtact atgaagagca cgacaagacc ggcaacaatt gaaatcaaaa gcccggtcca
     5041 tgtaaattta ttgaactttt tgaggtaata aaagatggcc aaagccggga atgtcaatat
     5101 actcaacaag tgaaccccta tggaaagacc agccataaaa agggaaaata ctatccatcg
     5161 gtcactgtgt tgtgtgtctg gaagataata ccatttcaat aaggaccaca atgtcaatgc
     5221 tgtaaaaaaa gtagacatgg catatacctc gccttccatc gccgagaacc agactgaaat
     5281 acagaaagtg gaagtaagtc cggcaactaa tccagccagc aataatccaa tgttctgccc
     5341 ggagtcggac tcattgcccc ttcctactaa ggacaattta cccaggtaca tggtcgtcca
     5401 gcaaataaac attccggcaa atgctgtaag aaggcctgac ataag
//
LOCUS       DAOQMC010000161         5432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_6776_length_5432_cov_4.712665, whole genome shotgun sequence.
ACCESSION   DAOQMC010000161 DAOQMC010000000
VERSION     DAOQMC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6776_length_5432_cov_4.712665"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="PKV31_07180"
     CDS             <1..1425
                     /locus_tag="PKV31_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNB92677.1"
                     /translation="LIIILISCHPGYGQTGAPLPVYPIPSPQQLAWHRMEMNAFIHIS
                     INTFTDQEWGYGNEPAMVFNPTAADPDQWAKALSENHFKGIILTAKHHDGFCLWPDEN
                     TEYSIRNSPYKDGKGDLVKETADAAAKYGMKFGIYLSPWDRNRGDYGSKSYIKYYRKQ
                     LKTLLTNYGPVFEMWFDGANGGDGYYGGKNTNVYVNKQRYYNWPKTIKTAEKLQPDIL
                     FFSDAGPDIRWVGNEKGQAGITNWNTINNDTLYAGKEGINAILASGSPDGKKWIPAEV
                     DVSIRPGWFYHDKENSKVKSPEQLFDIYLTSVGRGAVLLLNVPPDKNGLISDADLIAL
                     KGFSRLLKQRLGKNLAKNATVTASNVRGGAMEYAPERMLDDDMDSYWCTDDGVTSASF
                     DISLDQNQMVKYVLVQEYIPLGQRVKSFEIEALDGDQWKVVGQGTTIGYKRIIEIPET
                     ITSKLRIWIKDAKACPLISNVQVY"
     gene            complement(1523..1717)
                     /locus_tag="PKV31_07185"
     CDS             complement(1523..1717)
                     /locus_tag="PKV31_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92678.1"
                     /translation="MGRKKKDELSPRPAIDLDIHINEFGEIMHGFDIDKINEFLNQNT
                     KDWRIKAENENESPFFDEEE"
     gene            complement(1721..3241)
                     /gene="gltX"
                     /locus_tag="PKV31_07190"
     CDS             complement(1721..3241)
                     /gene="gltX"
                     /locus_tag="PKV31_07190"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNB92679.1"
                     /translation="MSVRVRFAPSPTGALHIGGVRTALYNYLFAKKHHGTFILRIEDT
                     DQNRLVEGAEEYIMDALRWAGLDPDESPDNPGDYGPYRQSERLDIYRKYAGELIESGK
                     AYYAFDTPDALENARKNAESSGGAFLYGSANRLNMHNSLSMSPEEVKQALDAGEHYVI
                     RLKVEADEVIEFTDLIRGQVQFSSNELDDKVLMKGDGFPTYHLANIIDDFLMRITHVI
                     RGEEWLSSTAHHILLYRAFGWEDQRPAFSHLPLILKPSGNGKLSKRDGDKLGIPVFPL
                     SWEHPDLGKFFGFREAGFLPEAMLNFIALLGWSPGENIEMMNMGEMIDHFSIEKIGKS
                     GSRFDFDKAQWFNQKYIMQSCADKLALLVEGRFREAGFHGEVAELERIIDLFKERVTN
                     INDFVTISRFIFTEPESFDTAPIIKKWNDAVAEVFLAIIDKIRTIQPFQDSVLEPEIK
                     GLIEAQGVGLGSIFPALRVALAGTAKGPDLFTMMELMGRNLVERRLENGFEMFNNK"
     gene            complement(3807..4931)
                     /gene="hppD"
                     /locus_tag="PKV31_07195"
     CDS             complement(3807..4931)
                     /gene="hppD"
                     /locus_tag="PKV31_07195"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNB92680.1"
                     /translation="MDTQIKESKEIGKDILPINGTDYIEFYVGNAKQAAIYYMAAFGF
                     EQVAYKGPETGSRDVVSYVLQQGKIRFVLTSALHPEHEISRHVALHGDGVKVLALWVD
                     DAVASWEGTTARGAESAFEPTVLEDEYGKVTVSAIKTYGETIHTFVQRSDYKGAFLPG
                     YKAWKSERKVEPLGFLYVDHCVGNVELGQMNQWVKFYEDVMGFRLLITFDDNDISTEY
                     TALMSKVVSNGNGYIKFPINEPAQGKKKSQIEEYLDFYKTSGVQHIAIATNDILKTVD
                     GLRKNGVEFLVVPGTYYEDLFDRLGEIDESIEELKRLNILVDRDEEGYLLQIFTKPVL
                     DRPTVFFEIIQRKGATSFGKGNFKALFEAIEREQAIRGTL"
     gene            complement(4989..>5432)
                     /locus_tag="PKV31_07200"
     CDS             complement(4989..>5432)
                     /locus_tag="PKV31_07200"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNB92681.1"
                     /translation="VGWDGYHYPYLFSIHNFEPITGRIHMPPPIHLTFVTKAAVICSF
                     VPRLYDYHPQSIPAPYFHSNIDSDEVLYYVDGEFMSRKHVEKGQITLHPGGVPHGPHP
                     GAIEKSIGAKETGELAVMFDTFSPLFLTKDAIDIEDPDYYRSWLE"
BASE COUNT         1613 a         1280 c         1061 g         1478 t
ORIGIN      
        1 ctaatcatta ttttaatatc ctgtcatccg ggatatggcc aaacaggcgc cccgctgcct
       61 gtctacccga tcccctctcc tcagcaactg gcatggcatc gtatggagat gaatgccttc
      121 atacatatct ccatcaatac gttcaccgat caagaatggg gatatggaaa cgaaccggca
      181 atggtattca accctacggc tgccgatccg gaccaatggg caaaagcact tagcgaaaat
      241 catttcaaag gaatcatact tacagccaag catcatgacg ggttctgcct ttggccggat
      301 gaaaataccg aatacagcat caggaatagc ccctataagg acggaaaggg tgacctggtc
      361 aaagagacag ccgatgctgc tgcaaagtat ggcatgaaat tcggtattta cctatcccca
      421 tgggatcgaa acagaggaga ttacggcagt aaaagctata ttaaatatta tcggaaacaa
      481 ctgaaaacac tactcaccaa ttatggcccg gtctttgaga tgtggtttga cggagccaat
      541 ggtggggatg gctattacgg aggcaagaat accaatgtat atgtaaataa gcaaaggtat
      601 tacaattggc ccaagaccat caaaaccgct gaaaaacttc agccggatat actttttttt
      661 agcgatgcag gccctgatat cagatgggta ggcaatgaaa aaggacaggc aggaatcacc
      721 aattggaata caatcaataa tgataccctc tacgcaggga aagaaggcat caacgccata
      781 ttagcctccg gatcacctga tggtaaaaaa tggattcccg cagaagtaga tgtttccata
      841 cggccgggat ggttttatca tgacaaggaa aattcgaaag tcaaaagccc ggaacaacta
      901 ttcgatattt acctgacttc tgtaggaaga ggagctgtcc tattgctcaa tgttccaccc
      961 gataagaatg ggctaatcag tgatgcagat ctgatagccc tgaaaggctt ttccagatta
     1021 ctaaaacaaa gactgggaaa aaatctggca aaaaacgcta ctgtaactgc ctccaatgtc
     1081 agaggaggag ctatggaata cgcaccggag agaatgctgg atgacgatat ggattcctat
     1141 tggtgtacgg acgatggtgt aacatcagcc tcatttgaca tcagtcttga tcaaaatcaa
     1201 atggtcaaat atgtcctggt ccaggaatat atcccgcttg gacaaagggt gaagtccttt
     1261 gaaatagaag cacttgatgg agaccaatgg aaagttgtcg gccagggtac aacaattgga
     1321 tataaaagaa tcattgaaat acccgaaaca atcacatcca aattgagaat atggattaag
     1381 gatgctaaag cctgcccgtt gatatctaat gtacaggtat attaatttcg gctaaaatca
     1441 aatctgactc catattagtt agttactgcc actaaaatca gggctgcaac cggactatac
     1501 gacagctata gaaacagaat tgctattcct cttcatcaaa aaacggagat tcattttcat
     1561 tttctgcctt gattcgccaa tccttggtgt tttgattgag gaattcattg attttgtcga
     1621 tatcgaaccc atgcataatc tcaccgaact cattgatgtg aatatccaga tcgatggctg
     1681 ggcggggaga taattcatct ttcttctttc ttcccattgc ctatttatta ttaaacattt
     1741 cgaatccatt ttcaaggcgt ctttcaacca aattacgacc catcaactcc atcatggtaa
     1801 aaaggtccgg cccctttgca gtacctgcca aagccactct taaagcagga aatatcgaac
     1861 ccaaccctac tccctgagcc tctatgagcc ctttaatctc gggttccagc actgaatcct
     1921 gaaatggctg tatagtcctt attttatcaa taatggcaag aaaaacttcc gctaccgcat
     1981 cattccattt ctttataatt ggagcagtat cgaatgattc aggttccgtg aaaataaaac
     2041 gggatatagt tacaaaatcg ttgatgttag tgacacgctc cttgaacaga tcgataatgc
     2101 gctccagctc agccacctct ccatggaatc cggcctctct aaacctgcct tccaccagca
     2161 aagccaattt gtcggcgcag gattgcataa tgtacttttg gttgaaccat tgagctttat
     2221 caaaatcaaa gcgacttccc gactttccga tcttttcaat ggaaaaatga tcgatcattt
     2281 cacccatatt catcatttcg atattctctc cgggactcca acccaataaa gcaatgaaat
     2341 tgagcattgc ttccggcaaa aaacctgctt ccctgaatcc aaagaactta cctaagtcgg
     2401 gatgctccca cgacaatgga aatactggta ttcccagctt atcgccatcc cgcttactca
     2461 gtttgccatt gccagaaggc ttcaggatga gtgggagatg agaaaaggca ggcctctgat
     2521 cctcccatcc aaaggcacga tatagcagga tatggtgtgc cgtgcttgaa agccattcct
     2581 caccacgaat gacatgggta atcctcatca gaaaatcatc gatgatattg gccagatgat
     2641 atgtagggaa accgtcacct ttcatcaaaa ccttgtcatc cagctcattg gaagaaaatt
     2701 gcacctgacc tctaatcaga tcggtaaatt ctatcacctc atcagcctcc accttcaggc
     2761 ggataacata atgctcacct gcgtccaaag cctgtttaac ctcttcagga gacatggaaa
     2821 gactgttgtg catgttgagc ctgttggcgg atccgtaaag gaaagcacca cctgatgatt
     2881 cagcattttt gcgggcattt tccaaggcat ccggcgtatc aaaggcataa tatgctttgc
     2941 ctgattcaat cagctcaccc gcatattttc tatagatatc caatcgttca ctttgtctgt
     3001 aaggcccgta gtcaccgggg ttgtcaggac tttcatccgg atccagcccc gcccatctca
     3061 atgcatccat gatgtactct tccgcaccct ccacaagtcg gttttgatca gtatcttcga
     3121 tccttaaaat aaaagtacca tgatgttttt ttgcaaaaag gtaattatac agcgctgttc
     3181 ggacaccccc gatatggagg gcgccggtcg ggcttggagc gaaccttact cttacggaca
     3241 ttatttctga attaattgaa acaaagttgc aaaaatatag aatttcctcc agtgaacggt
     3301 aaaaattagt cgtattggca tcttatacaa atcaatcaat ccaatagtat ctaaaataaa
     3361 tccaaagatt aaaaaggtca attaactaaa ggaatggacc agatctgccc atatccttta
     3421 taaaagatcc aattttgtaa attcctattt taaaactctc ctttcgggat taccattatc
     3481 cgggaaaaat tccattcgtg aaaagagact gaccaaagat atgtaatgcc tttttccaac
     3541 tgcgtcagtc gcataccatt aatacaaatc aactgtgtca aaccttccta tttcttgcaa
     3601 ataaagcagc cggacaactt tccttctaca taccgaatat caaaacaaca agcccatccg
     3661 taaaatagcg aatgggcttg aactattatt ctaaaaaagc ttcggcaatc gcaaaaattg
     3721 gttgtgcaaa taatccatga tttgaaatca catggatcca tgttctgcca attcaaccca
     3781 aagcattgaa atccatgaac cggaaattac aatgtacccc tgattgcctg ctctcgctcg
     3841 atggcctcaa agagagcctt gaaatttcct ttgccgaatg aggttgcccc ctttcgctgg
     3901 attatttcga aaaagacggt tggtctgtct aaaacaggct ttgtaaatat ctggagcaaa
     3961 tagccctctt catccctgtc cacaagaata tttaagcgct tcaactcctc gatggattcg
     4021 tcgatctcgc ccaatctgtc aaacaggtct tcataataag ttcctggcac aaccagaaat
     4081 tctacaccat ttttacgcaa gccatcgact gttttcagta tatcattggt tgcaattgca
     4141 atatgctgta ctccggatgt tttataaaaa tcaaggtatt cttctatctg ggatttcttt
     4201 ttaccttgtg cgggttcatt gatcgggaac ttgatgtaac cattgccatt ggataccacc
     4261 ttgctcatca aggcagtgta ttcggtagaa atgtcattat catcaaaagt aatcagcaat
     4321 ctgaaaccca tgacatcctc atagaatttt acccattgat tcatctgacc caattctaca
     4381 ttaccgacac aatgatctac gtaaaggaag ccaagtggtt ccacttttct ttctgacttc
     4441 catgctttat atcccggcag gaatgccccc ttataatcac ttcgctgaac gaaagtatgg
     4501 atggtctcgc cataggtctt aatcgctgaa accgttactt tgccatactc atcttccagc
     4561 accgttggct caaatgctga ttctgcgcca cgggctgttg tcccctccca tgaagcaact
     4621 gcatcatcga cccaaagggc cagtacttta actccgtcac cgtgcaatgc cacatgtctg
     4681 gatatttcgt gctcaggatg aagagctgaa gtcagtacaa aacggatttt cccttgttgt
     4741 agtacatagg agacaacatc cctgctccca gtctctggcc ctttataggc cacctgctca
     4801 aaaccgaatg cggccatata atatattgcg gcctgcttgg catttccgac atagaactca
     4861 atatagtcgg taccattgat tggcaatata tccttgccga tttccttact ttctttaatc
     4921 tgtgtatcca tgataaaatt ttgtattttt aaaaatattt agtcaaatcc aattcttatt
     4981 ccgcaagatc attccagcca actcctgtaa taatcaggat cctcgatatc gatggcatct
     5041 ttggtcagaa ataaaggcga gaatgtatca aacatgactg ccaactctcc ggtttcctta
     5101 gcaccgatac tcttctcgat ggcgccggga tgcgggccgt gtggtactcc acccggatga
     5161 agagtgatct gacctttttc cacatgcttc ctgctcatga attcaccatc cacatagtac
     5221 aatacctcgt cactgtcgat attactgtgg aaatatggcg ccggaatgga ctgtggatga
     5281 taatcgtaca atctcggtac aaaactgcag atgacggcag ctttggtaac aaaggtcagg
     5341 tgaataggcg ggggcatgtg aattcggccg gttatcggct cgaaattatg aatcgaaaac
     5401 agatatggat aatggtatcc gtcccatcca ac
//
LOCUS       DAOQMC010000162         5255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7149_length_5255_cov_4.542885, whole genome shotgun sequence.
ACCESSION   DAOQMC010000162 DAOQMC010000000
VERSION     DAOQMC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7149_length_5255_cov_4.542885"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKV31_07205"
     CDS             complement(<1..920)
                     /locus_tag="PKV31_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNB92682.1"
                     /translation="MTNKIIFSLVMAVSFQWSLSAQDVHFTRFYDAPTTLSPAKTGDY
                     LGTFRAGGIMRDQNYNLSHIYLTPAGYVDAPIIRGFRQNHWVGVGLTFLMDRAGAAGL
                     KNASFGASAAYHIGFNKKMTSYLSVGISYALAARSIADREKAIFEDGLINSIPSVDID
                     NINDQKVNYGFFSGGAQYTAALPNDGNIKAGFAMLYLNRPRNGILKTGGIRLPIRTNV
                     YATADASVADRIDILPALYFATMAGQRDMALQFQGGYRLNPRKGQRVMAGLGYRFGDA
                     IQVLAGMDIKNTRIGLAYDITVNDIRPTGAL"
     gene            complement(1277..2140)
                     /locus_tag="PKV31_07210"
     CDS             complement(1277..2140)
                     /locus_tag="PKV31_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB92683.1"
                     /translation="MKFGKTIKIFLIDGDPNGRMSCELSNWTGKAFKIPRIKIKDCVD
                     RPEIKNPGIYFLFGKNEIGKDIIYIGEAETVYSRLLQHLNQKDFWNEAIIFISKDENL
                     NKAHIKYLENKLYEIAKQVNRYHIDNTTVPTLSSISESDRAEMEEFLENVKLLVSTLG
                     HKAFDTKRETSSIKPKDVFYLKGPRGADAHGLPTSDGFLVLKGSKAAESTTPSIASNL
                     VKLRSKIIDQGAIILKKGYFEFPDDFIFSSASAAASIVLGRNANGLSEWKFQSGKTLK
                     DFETNDAINEV"
     gene            complement(2207..3895)
                     /locus_tag="PKV31_07215"
     CDS             complement(2207..3895)
                     /locus_tag="PKV31_07215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB92684.1"
                     /translation="MEVNSFEDLNLEDSLLKALKAIDYSVPSPIQARAIPVALTGVDI
                     IGQAQTGTGKTAAFGIPMIQNISKSGRYTKGLVLCPTRELCIQVTNELKKLAYFSRHV
                     SVFAIYGGDPINPQIKALKAGVDIVVGTPGRVMDHLSRGTLLLDGLEILTLDEADEML
                     NMGFREDLETILQSVPEHRQTILFSATMPKAILDITHLYQKNPQLIKVTPTNLTSASV
                     EQFYIETDMNHRLKVLTYLLNAGDYAQAIVFCNTKAKVDELSNELAENLVKNDVLHGD
                     LGQEKRNRILNKFRKGEFKVLVATDVAARGIDVPNVDLVCNYDVPFDPEYYVHRIGRT
                     GRAGKEGKAYSFLAGSRDKRLLRQIESFAKVKVEQYKLPTATEIARIELDKVKTQLKA
                     AILAGKEAVYLDLAKELMQEGGPANIIAALLLKIIPPGKHEWAVSAGASRDNKEKRGE
                     RPDSNRRKQDRERTRRDEKPKPSANRGKDKDRKSDKPKFGKPEKTFVKHENEMSLEDI
                     LASLGLDSEGNDIKPSGKDKKKTDKKKMPGDHFYSSSIREKEEFVPVPRKKKKK"
     gene            complement(4031..4459)
                     /locus_tag="PKV31_07220"
     CDS             complement(4031..4459)
                     /locus_tag="PKV31_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92685.1"
                     /translation="MMKRGFRYLGLALLCIVAILLWQAASDPIQVTRFSRAALKEIEV
                     RKQNYYKKKMAECHANALGMASTMVDSALIEQALFIGADTLLRPDRPMRPEVETFASN
                     IENIPVRPWLKKTDFVSPFQKKDTFVKDSLKLKDSVKVIK"
     gene            complement(4456..4761)
                     /locus_tag="PKV31_07225"
     CDS             complement(4456..4761)
                     /locus_tag="PKV31_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92686.1"
                     /translation="MRLLSPLVLLIFVLTSCSSDEDRMRPGEKYSVDTTMFREYQKID
                     SIVKIECDRITDSIFRKAYDSLLRIRSKDIRSIKGDGQPPVENRFPATLEKLRKKQG"
     gene            complement(4761..>5255)
                     /locus_tag="PKV31_07230"
     CDS             complement(4761..>5255)
                     /locus_tag="PKV31_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB92687.1"
                     /translation="SVEDNIKAVLEMMPYTTEQQRDKLESLLDEFGLNKVRKSNGDTL
                     SGGERRRCEIARALATDPKFILLDEPFAGIDPIAVEDIQYIVAKLKTKNIGILITDHN
                     VQETLSITDRSYLMFEGSILKAGTAEELAADEQVRKVYLGQSFELRRKVIDLSTKKEI
                     REGV"
BASE COUNT         1360 a         1229 c         1050 g         1616 t
ORIGIN      
        1 agagcaccgg ttggccggat atcattgaca gtgatgtcgt aagcaaggcc aatccgtgta
       61 tttttaatat ccatacccgc cagtacctga atggcatcac caaaacgata gcccaatcct
      121 gccattacac gctgaccttt acgagggttg agccgatagc ctccctgaaa ttgcagtgcc
      181 atatctcttt ggccggccat agtggcaaag tataaagcag gcaggatgtc gatcctatct
      241 gctacggatg catccgccgt ggcatataca ttggtgcgga taggaagccg gatgccaccg
      301 gtcttcagga tgccattcct tggacggttg agatacagca tggcgaaacc ggccttgatg
      361 ttaccgtcat ttgggagggc agccgtgtac tgagctcccc cggagaaaaa tccgtaatta
      421 actttctgat cgttgatatt atcgatatcc acggatggaa ttgaattgat caggccatcc
      481 tcaaatatcg ccttctccct gtctgcaatg gaacgggcag ccaatgcata ggaaattccc
      541 acagaaagat aggaagtcat ttttttatta aaaccaatgt gataggcagc tgatgcacca
      601 aaggaggcat tttttaatcc ggcagctcct gcccggtcca tcagaaaagt caatccgaca
      661 cctacccaat gattctgcct gaagccccgt atgataggcg cgtcaacata accggcggga
      721 gtaagataga tgtgtgacaa attatagttc tgatcacgca tgattccacc tgcccggaag
      781 gtaccgaggt aatccccggt cttagcagga ctcagcgtgg tcggtgcatc atagaacctt
      841 gtaaagtgta catcctgtgc tgacaatgac cattgaaagg acaccgccat cacgagtgaa
      901 aaaataattt tattggtcat acacgtaaat ttcgtggatt cgataagatt gggataaaag
      961 taaataaaat tatggtaagg aaaaaaataa aatatgacag catggatttc aaggcattga
     1021 catggcttct tttagtttca tccttcggtg tacccggata ttttatgttt ttcaacacat
     1081 ccttttcttt gtaaaaaaat tacatggatg atgggaggca aattgaatca aatgacactt
     1141 gcatacaagc tcctgttgcc tcacgtgtta aaatattaaa aatgttgcgc aaaacggtcg
     1201 ggtgcttacg cagtgccgga ttgttaagtt aagattttca tttaaaacga aaagccggtt
     1261 ttgactaagt accttgttat acctcgttta ttgcatcatt tgtttcaaag tctttaagtg
     1321 ttttaccact ttgaaatttc cactcgctta atccgttagc atttctacct aaaactatag
     1381 aagccgcagc ggaagcacta ctgaatataa aatcatcagg gaattcaaaa tatccttttt
     1441 ttaaaataat agccccttga tcaattattt ttgatctaag cttcaccaag ttggaggcga
     1501 ttgaaggagt ggtcgattcc gctgcttttg aacctttcaa tacaagaaat ccatcagagg
     1561 ttggtaatcc atgtgcatct gctcctctgg gtccttttaa ataaaagaca tctttgggtt
     1621 tgatcgagga agtttccctt tttgtgtcga aagccttatg cccaagtgta cttactaaaa
     1681 gctttacatt ttctaaaaac tcttccattt ctgcgcgatc agactcggag atcgacgaca
     1741 gagttggtac ggttgtgtta tctatatggt atctatttac ctgtttggca atttcataca
     1801 atttgttttc taaatactta atatgagcct tattaagatt ttcatctttg ctaataaata
     1861 ttatggcctc attccaaaaa tccttttgat taaggtgctg aagcaaacgg ctatatactg
     1921 tttcggcttc accaatgtaa attatatcct ttccaatttc gttttttcca aatagaaagt
     1981 aaattcccgg atttttaatt tccggcctgt caacacaatc tttaatttta attcggggaa
     2041 ttttaaatgc ttttcctgtc caattagaaa gttcacaact catccgcccg tttggatcac
     2101 catcaattag aaatattttt atagtcttgc caaatttcat tcttgagtat ttgttattac
     2161 aaagcgctaa atcatcaaac agaaaaacca atcacctgtc agagcattat tttttcttct
     2221 tcttccgggg cacaggtaca aattcttcct tttctcgtat agatgaacta taaaaatgat
     2281 cacccggcat tttcttttta tcagtcttct ttttgtcttt accggaaggt ttgatatcat
     2341 taccctcgga atccaaacct aaactggcca ggatatcctc caatgacatc tcattttcgt
     2401 gtttgacaaa agttttttcc ggttttccga acttaggttt atctgatttg cgatctttat
     2461 ctttgccgcg atttgcggat ggcttaggct tttcatctct ccgggtcctt tcccgatcct
     2521 gtttccgcct gtttgagtcc ggccgttcac ctcttttctc cttgttgtct ctcgatgcac
     2581 cggcgcttac tgcccattcg tgtttgccgg gaggtataat tttcaaaaga agggcagcaa
     2641 ttatgttcgc cggtccacct tcctgcatca actcttttgc cagatccaga tatacggctt
     2701 cttttccggc caggatagca gccttcagtt gcgtttttac cttgtccagt tcgattcgtg
     2761 caatttccgt ggctgtaggc aatttgtatt gttctacttt gaccttggca aagctctcaa
     2821 tctgacggag cagtcttttg tctcttgagc ctgccagaaa actatatgcc ttgccctctt
     2881 taccggcccg gcccgtcctg cctatcctgt gtacataata ttccggatca aacggcacat
     2941 cgtaattaca gaccagatcg acattgggaa catcgatccc ccgagctgcc acatctgtag
     3001 ccaccaatac cttaaactct ccttttctga acttattgag gatgcgattg cgtttttcct
     3061 ggcccaaatc tccgtgcaag acgtcgtttt ttaccagatt ttcggccaat tcattggaca
     3121 gttcatcgac cttggccttg gtgttacaaa aaacgatggc ctgggcataa tcgcctgcat
     3181 tgagcagata ggtaagtact ttcaggcgat gattcatgtc tgtctcaata taaaattgtt
     3241 ctacacttgc tgaggtaaga ttggtaggcg tgactttgat cagttgggga ttcttctggt
     3301 acaggtgtgt aatatccaga atagcctttg gcatggtagc cgaaaataaa atggtctgcc
     3361 tatgctccgg taccgattgc aaaatcgtct caaggtcttc cctaaagccc atattgagca
     3421 tttcatccgc ctcatccagg gtgagtattt ctaatccatc cagtaataat gtccctcgtg
     3481 ataagtggtc catcactctt cccggtgtac ccaccacaat gtccactccg gctttcaagg
     3541 ccttaatctg cggattgatg ggatcgccac catatattgc aaaaacggaa acatgcctgg
     3601 aaaaatacgc cagtttcttc agttcattgg taacctgaat gcataactcg cgcgtgggac
     3661 ataggacgag acctttggta tagcggccgg atttggagat attttggatc atcggaatac
     3721 cgaaagcggc tgtcttgcct gttcctgtct gtgcttgccc gattatgtcc acccctgtca
     3781 gggcgaccgg tatagctcgg gcttgtattg gactgggaac tgaatagtca atcgctttca
     3841 atgctttcaa caagctatcc tccaggttta ggtcttcaaa tgaatttact tccatggatt
     3901 tatatttggt ggttgtgttg gaatatttct ttccaataaa atgtattaca tgttaacgat
     3961 tgtcttacga gacaatcaga ttttgcgtct ttagtcaaag tgcagcccga caatcaataa
     4021 acctgaaaat tcattttatc accttcacgc tatccttcag cttcaaggaa tctttcacaa
     4081 aggtatcctt tttttggaaa ggactgacaa aatcagtttt tttaagccat gggcgtaccg
     4141 gaatattttc aatatttgaa gcaaaagtct cgacttccgg cctcataggc ctgtccggtc
     4201 tgagtagcgt atcggccccg ataaacaatg cctgctcgat cagagcagag tccaccatcg
     4261 tggaagccat tcccaacgca ttggcatggc actccgccat tttcttcttg tagtaattct
     4321 gtttcctcac ttctatttcc tttaatgcgg ccctggaaaa cctggtgacc tgtatcggat
     4381 cagatgctgc ctgccacaat aatatggcca cgatacagag caaagccaaa cccaaatacc
     4441 taaaccctct cttcatcatc cttgtttttt gcggagcttt tccagagtag ccgggaaccg
     4501 gttttctacc ggtggctgac cgtcaccctt gatggaacgg atatctttag atcttatccg
     4561 aagtaagcta tcataggcct ttcggaaaat actgtctgta attctatcac actcgatctt
     4621 gacgatggag tcaattttct gatattcgcg aaacatggtc gtgtcaacgg aatatttctc
     4681 tccgggcctc atccggtctt catcactact gcatgaagtc aatacaaata tcaacaaaac
     4741 taaaggactc aacaatctca tcaaacgccc tcccggattt ctttttttgt actcaggtcg
     4801 atgaccttac gccttaattc aaagctttga ccaagataca ccttccgtac ctgttcatcc
     4861 gcagcaagct cttcggcggt accggctttg aggatgcttc cttcaaacat caggtatgac
     4921 cgatccgtaa tggacaaagt ctcctggaca ttatggtctg tgatcaggat acctatattt
     4981 tttgtcttca atttggcgac aatgtattga atgtcctcca cagcaatcgg atcgataccg
     5041 gcaaagggct cgtccaacag gataaatttg ggatccgttg caagggcgcg tgctatctca
     5101 cagcgtcggc gttctccacc actcagggtg tcgccattgc ttttcctaac tttattgagg
     5161 ccaaactcat ccagtaacga ctccaatttg tcacgctgtt gctccgttgt ataaggcatc
     5221 atttccagaa cagccttgat attatcctcg acact
//
LOCUS       DAOQMC010000163         5171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7344_length_5171_cov_4.492963, whole genome shotgun sequence.
ACCESSION   DAOQMC010000163 DAOQMC010000000
VERSION     DAOQMC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7344_length_5171_cov_4.492963"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2531
                     /gene="ileS"
                     /locus_tag="PKV31_07235"
     CDS             <1..2531
                     /gene="ileS"
                     /locus_tag="PKV31_07235"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNB92688.1"
                     /translation="SIVMAADLVPTWFKVEGADAEMSYTAGQKLLPYRIVDEYKGKDL
                     TFIRYEQLLPYAQPDEGDAFRVIKGDFVTTTDGTGIVHIAPSFGADDRRAAMANGIGS
                     LTLVDRQGKFTDEAGPFAGRYVKDYKNEGENFKDVDVDIAILLKQENKAFNVQKFVHN
                     YPHCWRTDKPVLYYPLDSWFVRASAAKERMAELNRTINWKPEHTGSGRFGVWLENVLD
                     WNLSRSRYWGIPLPIWRTEDGSEEIIIGSKEELLQKAEEAFKAGILKIQDKSGAIFTT
                     NTDGHLTYREGFDLHRPYIDEVVLLSKQGKPMYREPDLIDVWFDSGSMPYAQWHFPFE
                     NLDKFKANFPSDFIAEGVDQTRGWFYTLHAIATMCFDSVAFKNVVSNGLVLDKDGMKM
                     SKSKGNVVDPFKTLDQYGVDATRWYMITNAQPWDNLKFDLEGIDEARRKFFGTLYNTY
                     SFFALYANIDGFTFSEAYMPLEKRPEIDRWIISKLNSLVKEVRELLSDYEPTNAGRKI
                     EQFVDENLSNWYVRLCRRRFWKGEYTEDKVAAYQTLYECMTTVSKLIAPIAPFFADWL
                     YRNLNGGSRLGKHEAVHLAYFPADNEDVIDKNLEKRMEYAQRISSLGLSLRKKEKIRV
                     RQPLQKLIVPALSLDFKTKVEAVRDLIEAELNIKELLVTADTEGLIKKGAKPNFQTLG
                     KRLGKQMKLAQNVISNLSQADIRQFEQTNHYVLETPDGDFDLTLEDVIITSEDIPGWL
                     VAVDNEITVALDIAITPELKAEGIARELVNRIQNIRKNSDFLVTDRINIKVEPIDMTK
                     EAVALFANYIANEVLADSISLEANEGEEVELLDDINIRIQVEKA"
     gene            complement(2606..4885)
                     /locus_tag="PKV31_07240"
     CDS             complement(2606..4885)
                     /locus_tag="PKV31_07240"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNB92689.1"
                     /translation="MRYFYTFLAIIAMYGWVAGQAFHGTTGSIADDVTTTTFTCEVSG
                     LPSSMNSVTFGIEQVCVDISHTWDSDLIIKLQSPSGRIVTLIEGAGGDSDDFKNTCLS
                     GSNKNDIFHGWGPFSGTYRPSGDMGLFNDGSNPNGTWKLLILDVYPEDPGKLNSWEIQ
                     FGSNPSKPYLFESSALPIIKIDTYGQSIPDDPKIPASFKIISNDNGALNYVNDTVYSY
                     SGNIGIEIRGSSSQSFPKKSFGLELWDENGEDIKASVLGMPKESDWVLNAHYTDKTLF
                     RNNMTYKLFSEMGHYASRTQLVELFINGEYRGVYTFMEKIKRDKNRVDIPKLSENDTS
                     GMALTGGYIFKIDRINGSGTDGWTSKFEIPGRPFEYTNFLFEYPKDDKINNAQKAYIE
                     SFVDSFETALHSDDFQNPVNGWRKYADELSFMDFMLIQEMSKNVDAYRLSTFMYKTRA
                     DADNKIHFGPVWDFDIAWFNANYCETARPDGWVFNINFICPDGRSPFWWSKILTDTTF
                     ARNMRCRWESLRQTTLSEDHIASIIDGFVAEMQGAENHNFDRWPILGIWVWPNTDPIP
                     TTYQGEIDKLKGWIHQRFEWMDHAIRALSPDISLSINATNDSGLQWHFTPNIDLEGEY
                     KWDFGDGSTSSDVKPDHVFSAPGTYTVTLTYTNGFGCTESVTLTVNVEHSANYGFGDT
                     GVSIFPNPATETISLKTALAFESGNYQISDVHGRKMQSGKILNSGENNIAIGALHPGV
                     YILSLKIDNRAIQQRLVKM"
     gene            complement(4905..>5171)
                     /locus_tag="PKV31_07245"
     CDS             complement(4905..>5171)
                     /locus_tag="PKV31_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92690.1"
                     /translation="VGKNVSLHLKVFPNPTSDGRLVQVETGEAIEIYRMEIYDNRGRV
                     VLSGKGNVDKLNHLLRKNLQIAPGAYFIKLTAKHSILSSSFTIQ"
BASE COUNT         1520 a         1145 c         1130 g         1376 t
ORIGIN      
        1 aaagcatcgt tatggctgct gatcttgttc cgacatggtt caaggtggaa ggcgctgatg
       61 ctgagatgtc ctatacagca ggccaaaaac tgctgcctta cagaattgtt gatgaatata
      121 agggtaaaga ccttactttc atccgatacg aacaactact tccttacgct caaccggatg
      181 aaggtgatgc cttccgcgtg atcaaaggcg actttgtaac aactactgac ggtaccggta
      241 tcgtacacat agccccttcc ttcggtgctg atgaccggcg ggcagccatg gccaacggga
      301 taggcagtct cacccttgtg gacagacaag gtaaatttac ggatgaagcc ggcccattcg
      361 ccggtcgata tgttaaggac tataaaaatg agggcgaaaa tttcaaggat gtcgatgtgg
      421 atattgccat cctgttgaaa caggaaaata aagcattcaa tgtgcaaaaa tttgtccata
      481 actatccgca ttgctggagg acggacaagc cggttcttta ctatcctctg gacagctggt
      541 ttgtaagggc ttccgcagcc aaggaaagga tggcggagct caacagaaca atcaattgga
      601 aaccagaaca taccggatca ggacgatttg gcgtctggct ggagaatgtg ctggattgga
      661 acctgagccg ttcgaggtat tggggtatcc cattgcctat ctggcgtact gaggatggta
      721 gtgaagaaat catcatcgga tccaaagaag aattattgca aaaagccgaa gaagccttca
      781 aggcgggaat tttgaagatc caggataagt ctggtgccat ctttacaacc aataccgacg
      841 ggcatttgac atacagggaa ggatttgatc tgcaccgacc ttatattgac gaagtcgtac
      901 ttttgagtaa acaaggtaag ccaatgtacc gggaaccaga cctgatcgac gtttggttcg
      961 attccgggtc aatgccctat gcacaatggc actttccttt tgaaaatctc gataaattca
     1021 aggcaaattt cccgtcagac ttcattgcag agggtgttga tcagacacgc gggtggttct
     1081 atacccttca tgcgatagcc acgatgtgtt ttgacagtgt agcattcaag aatgtagtct
     1141 ccaacgggct tgttctggat aaggacggaa tgaagatgtc caaatccaag ggaaatgtcg
     1201 tggatccatt caagacattg gatcaatacg gtgtggatgc cacacgatgg tatatgatta
     1261 ctaacgcaca gccatgggac aatcttaagt tcgatctgga gggaattgat gaggcaagaa
     1321 ggaaattttt cggtaccttg tataatacct attccttctt tgcattgtat gccaatatcg
     1381 atggattcac attttcagaa gcatacatgc ctctggaaaa aagaccggaa atcgaccgct
     1441 ggatcatctc caaactcaac tcacttgtca aggaagttag agaattacta tcggattacg
     1501 agccaacaaa tgccggccgt aaaatcgagc aatttgtcga tgaaaacctg agcaactggt
     1561 atgttcggct ctgcagaagg agattctgga agggtgaata caccgaagac aaggtggcgg
     1621 cctatcagac cttgtatgag tgtatgacga ccgtctcaaa actcatcgca cccattgccc
     1681 cattctttgc ggactggctg tatcgtaatt tgaatggcgg gtcccgactg gggaagcacg
     1741 aggcggtaca tctagcctac ttccccgctg acaatgagga tgtcatcgac aaaaacctcg
     1801 agaagagaat ggaatatgca caacgaattt catccttggg tctttcactt cgtaaaaaag
     1861 agaagatccg tgtacgtcaa cctttacaga aactaattgt accggcactg agcttagatt
     1921 tcaagactaa ggtagaagca gtaagggatt taatagaggc agagctgaat atcaaggagt
     1981 tgctggttac tgccgatacg gaaggtttga ttaaaaaagg cgctaaacct aattttcaga
     2041 cgcttggaaa acgactgggc aagcaaatga aacttgcaca gaatgtgatt tccaacttgt
     2101 cacaggcaga tatcaggcaa tttgagcaaa caaaccatta tgtactcgaa actccggatg
     2161 gagattttga tctgaccctt gaagatgtca tcatcacttc cgaagatatt ccgggttggc
     2221 tcgtagcagt ggacaacgaa atcactgtcg cattggatat tgccatcact cctgagctaa
     2281 aagcagaagg aatagcacgt gaattggtca ataggatcca gaatatacgc aaaaacagcg
     2341 acttcctggt taccgacagg attaacatca aggtcgaacc tatagatatg acaaaggaag
     2401 cagtggcttt gttcgctaac tacattgcca atgaggtgtt ggcagacagc atcagtttag
     2461 aggcgaatga gggtgaggaa gtggaattgc tggatgatat caatatcaga attcaggttg
     2521 aaaaagcctg ataggttata ccaggtgcaa tagaaatgat gtttagagcc tattaatgag
     2581 ctctaaacat cattcaatca caagatcaca tcttcactaa tctttgttga atcgccctgt
     2641 tgtcaatctt taaggacaaa atatacacac ccgggtgtaa tgctccgatt gcaatattgt
     2701 tttcaccgga attaaggatc tttccggatt gcatcttcct cccatgcaca tcgcttattt
     2761 gatagttgcc cgattcaaaa gcaagtgcgg tcttcaggct gattgtttca gtcgcagggt
     2821 ttggaaagat ggatacaccg gtatcaccga agccatagtt ggccgaatgt tcaacattaa
     2881 ccgtcaaggt gacactctca gtacaaccga agccgttggt ataagtaagt gtcacagtat
     2941 aagtacccgg cgcagaaaat acatgatccg gtttgacatc gctggatgtg ctgccatctc
     3001 caaaatccca tttgtactcg ccttccagat caatatttgg cgtgaaatgc cattgcagac
     3061 ccgaatcatt ggtagcatta atagataggc taatatccgg acttaacgca cggattgcat
     3121 gatccatcca ttcaaatctt tgatgaatcc aacctttcaa tttatctatc tcgccctggt
     3181 aagtagttgg aatcggatct gtattaggcc atacccatat gccaagaatg ggccaacgat
     3241 caaaattgtg attctctgca ccctgcatct ccgctacaaa accatcgatg atcgaagcta
     3301 tatgatcttc cgacaatgtt gtctggcgca aggactccca gcggcaacgc atgtttctgg
     3361 caaaggtagt gtccgtaagt atctttgacc accagaatgg tgagcgacca tccggacaga
     3421 taaagttgat attgaacacc cagccatccg gcctggcagt ctcacaataa ttggcattaa
     3481 accatgctat gtcaaagtcc cataccggac cgaaatgtat cttattatct gcatccgccc
     3541 tcgtcttata catgaaagta ctaagccgat atgcgtcaac attcttggac atctcctgga
     3601 tcagcatgaa gtccatgaag gaaagttcat ctgcatactt gcgccatcca ttcaccggat
     3661 tctgaaaatc atcactgtgc aaggctgttt caaagctatc cacaaaggac tcgatataag
     3721 ctttttgggc attatttatt ttatcatcct tgggatattc aaaaaggaaa ttggtatact
     3781 caaaaggtct cccgggtatt tcaaactttg atgtccagcc gtccgtgcca ctgccattga
     3841 tccggtcaat cttgaagata taaccccccg taagtgccat tccagatgta tcattttccg
     3901 ataacttggg gatatcaacc cggttcttat cccgtttgat tttttccata aaggtatata
     3961 cgccccgata ttcaccattg ataaacaatt caaccaattg tgtccgggag gcataatgcc
     4021 ccatttcact gaaaagcttg taagtcatat tgttccggaa gagcgtttta tctgtataat
     4081 gagcatttag cacccaatcg ctttctttag gcatacccag tactgatgcc ttaatatcct
     4141 ctccattttc atcccaaagt tccaatccga aagatttctt aggaaatgac tgggaactgg
     4201 aacctctgat ctcaatacca atattgcccg aataggaata aacggtatca ttgacataat
     4261 tgagggcacc gttgtcattg gaaattatct taaaagacgc cggaatctta ggatcatcag
     4321 gaatggactg accgtaagta tctattttga tgattggcag tgcactgctt tcaaacagat
     4381 agggtttgga cggatttgag ccaaactgga tctcccaact attcaatttt ccgggatctt
     4441 caggatagac gtccaaaatc agaagtttcc aggtgccatt cgggttgctt ccatcattaa
     4501 aaagccccat atcaccacta ggtctgtaag ttcccgaaaa cgggccccac ccatggaaaa
     4561 tgtcattctt gttacttccg ctgaggcatg tattcttaaa atcatctgaa tctccacctg
     4621 caccttcaat cagggtcacg atcctgccgg acggtgattg aagctttatt atcaggtccg
     4681 aatcccaggt atgggaaata tccacacata cctgttcaat gccaaatgta actgaattca
     4741 tggaagaggg caaaccactc acttcacagg taaaagtagt ggttgttaca tcatccgcaa
     4801 tacttcctgt ggtaccatgg aatgcctgac ctgcaaccca gccatacata gctataatgg
     4861 ccaggaatgt ataaaaatat ctcatttgca atttaattta atatttattg aattgtaaaa
     4921 gaactactta gtattgaatg cttagctgtt aatttgataa aataagcgcc cggagcaatt
     4981 tgaagatttt ttctaagcaa atggttgagt ttgtcgacat tacccttacc cgacaacact
     5041 accctccccc tattgtcata tatttccata cgataaatct caatggcctc ccctgtctca
     5101 acctgaacca aacgtccatc agaggttgga ttggggaaaa ctttaagatg taaagaaacg
     5161 ttcttcccca c
//
LOCUS       DAOQMC010000164         5162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7375_length_5162_cov_4.051106, whole genome shotgun sequence.
ACCESSION   DAOQMC010000164 DAOQMC010000000
VERSION     DAOQMC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7375_length_5162_cov_4.051106"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /gene="dacB"
                     /locus_tag="PKV31_07250"
     CDS             complement(<1..1403)
                     /gene="dacB"
                     /locus_tag="PKV31_07250"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNB92691.1"
                     /translation="MLFFVATNDVLSQPVKEIVNKLATDASLKSAGLSFTIIDKASGG
                     LIYSHNPHLALVPASSMKLLSCAYGLKTLGKDFRFRTDLEYTGSIDSDGVLRGDLIIK
                     GYGDPSLGSEMTNGAQDLDRILNLWVDAIRKKGIRKVEGRIYGDATFLSDEGIYDSWQ
                     WADIGNYYAAGAYGLNIFDNLFKLKLKQTKQGGSPLVRTTNPNIPGLNFANSLICAGP
                     TSGDNAYIYGGPLQYQRAIRGTIPAGTGEFEIKGSQPDPPATAAMLLRQKLALAGIES
                     RSFAGSLTSNIPEGKRIMIESFYSSPLKEIVTQTLLKSLNLNAEGIVRYCHAVKNPGS
                     KIEDALKSFNQFLESQSGQRHGFIVADGSGLSSQNCISSFGFAGFLQEMFKDKAISGV
                     LTDALPVAGVSGTLKGYLKNSKAAAKVKAKTGSMNQVRSFSGLLTGASGKEYVFSLII
                     NNYTCNSAQIKSKVEAL"
     gene            complement(1535..2479)
                     /locus_tag="PKV31_07255"
     CDS             complement(1535..2479)
                     /locus_tag="PKV31_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNB92692.1"
                     /translation="MTDFFNINGELIAPGQRTVVRVPIGHLPVGTRLDMDVHVFRSHK
                     PGPVLLLTGGIHGDEINGIEILRRALKNGLFDELRCGSVIAIPLVNVFGFISFTRDST
                     DGKDINRSFPGSSTGSMASQIARKLTRQILKYCTAGIDFHSGSQSRFNYPQIRYTERH
                     ASAFELANAFNAPFVVKKTPLLNSLRKTAFDMGIPIVVFEGGETLRVDDFVVESAFDG
                     LKKVMNHLGMADFPVSTLQSRVLHKMSWLRSGCSGLFYPEVDNGSPVVKGQLLGWIHD
                     MMGNVSKPVISKSSGYIICVNNRPVVYSGDPLFNIGSA"
     gene            complement(2476..3357)
                     /locus_tag="PKV31_07260"
     CDS             complement(2476..3357)
                     /locus_tag="PKV31_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HNB92693.1"
                     /translation="MKVLILSRNIGLYSTQRLILECRKRGHNVQVIDPLTCNLIIDNN
                     ASYILDNSGQEVIADWVLPRIGSTATSYGAAVIRQFELKGIPVVTSSQALLQARDKLV
                     ALQLLAQNGLPIPKTALLSPIECDSQLIRREFEFPAVIKLLDSTHGLGVILSDSSQNG
                     VAIAEAFMRMQQKFIIQKFVRESAGQDIRAFVVNGKIIATMLRKAPEGDFRSNMHRGS
                     TATVIELTEEEQKVVLDASKTLGLEVAGVDIMRSKEGPVVLEVNASPGLEGIEGVTKV
                     NVAGSIVAYGDFIARTK"
     gene            complement(3484..4248)
                     /locus_tag="PKV31_07265"
     CDS             complement(3484..4248)
                     /locus_tag="PKV31_07265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB92694.1"
                     /translation="MDQPFLSIVTVCYNAAAVLEGTIQSVINQTCDDYEYIVIDGASK
                     DDTLNIIEEFSEHITKWITEPDKGIYDAMNKGLELASGRYVWFLNAGDHAYDERVVEK
                     LKAISGDCDVLYGEVMIVDEKRMQLGTRSKITVHKLPEDLTRQDMKRGMIVCHQGLIP
                     KLEITPHFILHNLSADIDWAIRILDKANVTQKTDFIISEYLAGGVSKKKWKQSLKDRF
                     AILKSHFGMANAIYNHIIIVARAIAHNFKRRGKNKY"
     gene            4438..>5162
                     /locus_tag="PKV31_07270"
     CDS             4438..>5162
                     /locus_tag="PKV31_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92695.1"
                     /translation="MKQTFSKLWPHMAAIIGIALFCIFYFLPENAENKVLPQGDVQHS
                     LSMQSEIRKYMAEEGREILWTNSMFSGMPSYQIFGANGKTYDFVPRIVYSSMQLTKGI
                     SSPTGLLFACCIGFYFMMLCFRFNWKYALVGSILFGMSTSFMHLIGNGHVNKVMVLAL
                     LAPTIGSMWLIYQKKYLLGSALTALFVNLQIMTNHPQISYYFAFLAVFFVIGVGIHLL
                     KQKEIKTLLIGTALLTLSSVIGVL"
BASE COUNT         1476 a         1167 c         1005 g         1514 t
ORIGIN      
        1 aacagtgctt ccaccttgga ttttatctgt gctgaattac aggtgtaatt atttataatc
       61 agggaaaata catattcctt acctgaagca ccagtgagaa gtcccgaaaa acttcgtacc
      121 tggttcatgg agcctgtttt agccttgacc tttgctgccg ctttggaatt tttcaaatat
      181 cctttaaggg ttcctgatac tcctgccacc ggaagtgcat ctgtcaaaac acctgaaatt
      241 gctttatcct tgaacatttc ctgcaaaaag cctgcaaatc cgaagcttga aatacaattt
      301 tggcttgata gaccggaacc atcggccact ataaagccat gtctctgacc ggattggctc
      361 tcaaggaatt gattaaagga cttcaaagca tcctcaatct tactgcctgg attttttaca
      421 gcatggcaat accttacgat cccttccgca ttgagattga ggcttttgag caatgtttgt
      481 gtcacaatct ctttaagagg tgaagaatag aaggattcga tcatgattct tttcccctcc
      541 ggtatattac tggtaagaga ccctgcaaat gaacgcgatt caatgccagc caacgccaat
      601 ttttgccgaa gtagcatcgc tgctgtagcc ggcggatcgg gttgacttcc cttgatctca
      661 aattcacctg taccggctgg tattgttccc ctgatagctc gctgatattg taatggaccg
      721 ccataaatgt aagcattatc accgctggtg ggacctgcac atatcagact gttggcaaag
      781 tttaatccag gaatattggg attggttgtc cggactaacg ggcttccacc ctgctttgtc
      841 tgtttgagct ttagtttgaa tagattgtcg aatatattca gaccataagc acccgcagca
      901 tagtagttgc ctatatccgc ccattgccag ctatcgtaaa tcccttcatc cgaaaggaag
      961 gttgcatcac cgtagatacg gccctctact tttcttatac ctttcttcct gattgcatca
     1021 acccatagat tcaggatacg atcgagatcc tgtgcgccat tggtcatctc tgaaccaaga
     1081 gatggatcac cataaccctt gataatcagg tctcctctca gtacaccatc actatcaata
     1141 cttccggtat attccaggtc ggtcctaaac ctgaaatcct tacccaatgt tttcagccca
     1201 taggcacaag aaagaagttt catggaactg gccggaacga gcgcaagatg gggattgtgg
     1261 gaatatatca atccaccgga agccttgtcg ataatagtaa aactcagacc agcactttta
     1321 agggatgcat ctgtagctaa tttgtttaca atttccttga caggttgact taatacatca
     1381 tttgttgcta caaaaaacaa catgtataaa taacataaac atttggtaat gtatgcatta
     1441 aattgtttta cctttttgca tgacaatctg ttagtactat tgttggaatt ataaaataac
     1501 atagacaaaa atcattagca ccattaaaaa aacatcaggc agaaccaata ttaaacaaag
     1561 ggtcaccgga ataaactacc ggtcggttgt tgacacatat aatatagccg gatgatttac
     1621 tgatgactgg tttactgaca ttacccatca tatcatggat ccagccgagc aattgacctt
     1681 tgaccacagg actaccgtta tccacctcag gataaaacaa acctgagcag ccggatcgca
     1741 gccaggacat cttatgtagg acacggcttt gcagagtaga taccggaaaa tcggccatac
     1801 caaggtgatt catcaccttt ttaagaccat caaaggctga ttccacaaca aaatcatcca
     1861 ctctcaatgt ctctccacct tcaaagacta cgatgggaat tcccatatcg aaggcagttt
     1921 ttctgagaga gttgagcaat ggtgtctttt tgacaacaaa tggcgcatta aaggcattgg
     1981 caagttcaaa agcagaggca tgtctttccg tatatcgaat ctgaggataa ttaaacctag
     2041 actggctacc ggaatggaaa tcaatgcctg ccgtacaata ttttagaatc tgacgagtca
     2101 gtttcctggc aatttgtgag gccatggaac cggtggatga acccggaaag ctgcgattga
     2161 tatcttttcc atccgttgaa tctcttgtaa aactgatgaa tccgaataca ttaaccaatg
     2221 ggatcgcaat taccgaacca cacctcaatt cgtcaaacaa gccatttttt aaagccctgc
     2281 gcaatatctc tattccattg atctcatcgc catggattcc tccggtaagt agtaaaaccg
     2341 gacctggctt atgtgaccgg aacacatgca catccatatc caatcgggtg ccaacaggca
     2401 aatggcctat cggaacacga acaactgttc gctgccccgg agcaataagt tcaccattta
     2461 tattaaagaa gtcggtcatt tagtcctggc aataaaatca ccatatgcga caatagatcc
     2521 tgcgacattg accttggtca ccccttctat tccttcaaga cccggactgg cattgacttc
     2581 aagtacaaca ggaccttcct tactacgcat aatatccaca cctgctacct ctaaacccaa
     2641 agtctttgaa gcgtcaagaa caactttctg ctcttcttct gtcaattcga tgaccgtggc
     2701 agtagaaccg cgatgcatat tacttcgaaa atcaccttca ggggccttcc tcaacatagt
     2761 tgcaataatc ttgccgttta ccacaaaagc cctgatatcc tggcctgccg attccctgac
     2821 aaatttctga atgataaatt tctgttgcat cctcataaag gcttctgcaa tggcaacgcc
     2881 attctgcgag gaatcagaaa gtataacacc aaggccatgt gtactgtcca ataacttgat
     2941 gacagcagga aactcaaatt cccgcctgat caattggcta tcacactcaa taggactcag
     3001 caaggcagtt ttagggatgg gaagcccatt ctgagccagt aattgcaagg caaccaactt
     3061 gtctcttgcc tgcaaaagtg cctgagagga agtaacaacc ggaatacctt tcaattcaaa
     3121 ttgtcggata accgctgcgc cgtagctagt agcggtggaa ccaatccgtg gcaaaaccca
     3181 atcagctatt acctcctgac cggaattgtc caaaatgtaa gaagcattgt tgtcaatgat
     3241 tagattacag gtaagagggt caatgacctg gacgttatga ccacgttttc tgcattcaag
     3301 aatgagtctc tgcgtggaat acagtccgat atttcgagat aggattaaga ctttcattta
     3361 aatatgtttg ccctgcaaaa tgaggatatt ttgtcaatta aatcgaattt tttttatttt
     3421 cctgatttaa aaatattagc cgggaaggta ttgaccttca aaagtcagat tgaaattagt
     3481 gttttaatat ttgttctttc ctcttcgttt gaaattgtgt gctatagccc ttgcaactat
     3541 tataatatgg ttataaattg catttgccat gccgaaatga ctcttgagga tggcaaagcg
     3601 gtccttgagc gattgcttcc attttttctt tgaaactccc ccggccagat attccgaaat
     3661 aatgaagtcg gttttttgcg ttacattggc cttatccaga atccggatag cccaatcaat
     3721 atcagcacta agattatgaa ggataaagtg tggcgtaatc tccagtttag gtatcagccc
     3781 ctgatgacac acgatcatac ctcttttcat gtcttgtctg gtcaggtctt ccggtaattt
     3841 gtggaccgtt atctttgagc gtgtgccgag ttgcattctt ttttcatcga ctatcatcac
     3901 ttcgccatag agaacatcac aatctccgga aatagctttt aacttctcca ctaccctttc
     3961 atcatatgca tgatctcctg cattcaggaa ccaaacatac cgaccggaag cgagttcaag
     4021 gcccttgttc atcgcgtcat atataccttt atcgggctca gtaatccatt tggtgatatg
     4081 ttcagagaat tcttcgatga tattcagcgt atcatccttg gaggcaccgt caatgacgat
     4141 atattcgtaa tcatcgcaag tctggttgat gacactttga atggtccctt ccagaacagc
     4201 agcagcatta taacaaacgg ttacaatcga taaaaaaggt tgatccaatc aataaatttt
     4261 ccgcaaaatt aatggtaaat tgcaggattg ccaaggtaat tgatctttat ttattcggga
     4321 ttcatctcca tgtcgtatta tagagccatt cttcaaaaat ggaattctaa agcatctgtt
     4381 ttttgctcaa tatcacgcca gagaattatc tttggcattt aattctaaag tatcaaaatg
     4441 aaacaaacct ttagcaagct gtggccacat atggctgcaa tcatcggtat agcattattc
     4501 tgtatatttt atttcttacc tgaaaatgct gagaacaagg tactcccaca gggtgacgtc
     4561 cagcactcac tgagtatgca gagtgagatc agaaaatata tggctgaaga gggtcgggaa
     4621 atactttgga ccaacagcat gttcagcggt atgccgagct accagatatt tggagccaat
     4681 ggcaagactt atgattttgt cccaagaatt gtctattcct ccatgcaatt gaccaaagga
     4741 atttccagtc cgaccggctt attatttgct tgttgtattg gtttttattt catgatgctt
     4801 tgtttccgat tcaattggaa atatgcactc gtaggttcca tcttatttgg catgagtaca
     4861 tcgtttatgc atcttatcgg caatggccac gtcaataagg tcatggtctt ggctctgttg
     4921 gctcctacta tcggatccat gtggctgatt taccaaaaga aatatttgtt ggggagtgcg
     4981 ctaacagccc tcttcgtcaa tctgcaaatt atgaccaacc accctcagat tagttattat
     5041 tttgccttcc tggcagtctt tttcgtcatt ggagtcggca ttcacctttt gaaacagaag
     5101 gaaataaaga cgttgctgat aggtaccgca ctactaacct tatcatccgt gattggagta
     5161 ct
//
LOCUS       DAOQMC010000165         5142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7421_length_5142_cov_4.412424, whole genome shotgun sequence.
ACCESSION   DAOQMC010000165 DAOQMC010000000
VERSION     DAOQMC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7421_length_5142_cov_4.412424"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKV31_07275"
     CDS             complement(<1..600)
                     /locus_tag="PKV31_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB92696.1"
                     /translation="MQFSLTEEHLAVRDTARDFAQKELLPGVIERDSKMIFPTEQVKM
                     MAELGFLGMMVSPEYGGGGMDTISYVLAMEEISKVDNSCSVLMSVNNSLVCWGLEAFG
                     TEEQKQKYLTRLATGEIIGSFCLSEPEAGSDATSQRTTAVDMGDYYLLNGTKNWITNG
                     GSSKIHLVIAQTHPEKGHHGINAFIVETGWDGVVIGAKED"
     gene            complement(855..1562)
                     /locus_tag="PKV31_07280"
     CDS             complement(855..1562)
                     /locus_tag="PKV31_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNB92697.1"
                     /translation="MNTRESEIRHNLAVIHERIRKACRDCNQAEEDVNLLLATKTVSP
                     DDIRIAVEAGATLIGENKVQEFSSKADALADLCITRHFIGHLQRNKVKEVLRHVQCIQ
                     TIDNLALAEEVNKRAGAMGRSVEVMIQVNTSFEASKFGIAPENLPELAEQVRNMQNLT
                     LIGLMTIGLNAEVIEDARPSYALLKELGEKLNDKYPEKPIREYSMGMSGDLEIAIEEG
                     ATIIRVGSAVFGTRIYP"
     gene            1950..3125
                     /locus_tag="PKV31_07285"
     CDS             1950..3125
                     /locus_tag="PKV31_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB92698.1"
                     /translation="MEQTDKIDEFLKVLAEATQASSFNSLTLSKPREKHGKIKSLYFR
                     PVMIRNEVKTQMVVRFSDREDTKNFSQTEFLAFASQSTRQHFFYAHLVTSSQEGHLLI
                     SRRGFVTLKWKQLTREMIALRVHDKEKQYIIPPEEPFLQHLGISSSTGTIIKDQYKKY
                     RQISRYIELFAPLTEGLDKTVPLEVTDMGCGKGYLTFAMHRFLKGAGFKQVHTSGIDI
                     KSDVIRNNNIIAREVGYDGLEFLHGRIEENRDINPDILIALHACDTATDDAIFYGVER
                     KAKVIVVAPCCHKQVRKSMARNELTSGLFRHGIIQERWASDLTDMIRANILRYLGYQV
                     KIMEFVSIEHTPKNIMITAQYYGKINETARQEIYTWMEMFGIGEQYLLNKLGLAPDN"
     gene            3720..>5142
                     /locus_tag="PKV31_07290"
     CDS             3720..>5142
                     /locus_tag="PKV31_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNB92699.1"
                     /translation="MFTLIVAFSSVQSGVSQNPFPVWPDSIFSAYYHQRAGLFNVMSG
                     TKGGVYFVGNSITDGAEWSEIFGDANIRNRGISADISAGVLNRIQTLIERRPDKVFLM
                     IGINDLARGIAPDSILHNIGTFVRLLREYSPTTQIFIQSLLPVNPDLKMFAQHTSKAA
                     EIKYVNGILEREAGQGYAYIDLHTHFLDEDGKLSRRWTNDGLHLNGDGYMLWKHIVFP
                     LVYDLQAHPAIIPQPLDISWLEGSFPLYKLKNITIPRSGLDNEAVILKDFLGSLGLYP
                     RIIPTGSPEGPVIVLNLDPAYDGPSQEAYSIMVSPQRVEIRAKGAHGILHGIQTLKQL
                     CRDGVMVPCCIIKDAPAFQWRGFMIDVGRNYVSPDILKQIIDKMSFYKFNIFHFHPTE
                     DIAWRLESRLYPQLTAPEYMLRDKGLYYTVAEVKSLIEFCRARHITFVPEIDMPGHSA
                     AFRRAMGVDMQSDSGTVLVKKIIG"
BASE COUNT         1405 a         1077 c         1142 g         1518 t
ORIGIN      
        1 gtcttctttt gctcctatga ctacaccatc ccacccggtc tcgacgatga aggcattgat
       61 tccgtggtga cctttttcag gatgcgtctg agctatcact aaatgtatct tactgctgcc
      121 accattggta atccagtttt tggtgccatt gagcagataa tagtcaccca tatcgaccgc
      181 tgtagtccgc tggctggttg catctgaacc ggcttccggt tcgcttaggc agaaggagcc
      241 gatgatctca cctgtagcca gtcgggtcag atatttttgt ttttgttctt ccgtaccaaa
      301 agcctcaagt ccccaacaaa caaggctgtt gttgaccgac atcaacacac tgcagctgtt
      361 gtccaccttg gagatttctt ccatcgccaa tacataacta atggtgtcca taccaccacc
      421 gccgtattcc ggagagacca tcataccgag aaatccaagc tcagccatca ttttaacctg
      481 ttccgtagga aaaatcattt tgctgtctcg ttctatgacg cccggtaata attccttctg
      541 ggcaaaatcc ctggccgtat cccgtaccgc caaatgttct tcagtaagtg aaaattgcat
      601 gtcgtatttt gttgaatgtg tgcaaagata ttattaattt aaaaatgaaa tcgggatttc
      661 atagtattta ttcaagttaa attggaggca catagaattt ttagcattca tttcgtttgt
      721 ctgaataacc ttgatgtatt cgacaaagca tgttggctca aaggggtgaa ttgttgtttt
      781 gaatgaggaa atggaacggt aattggaatc tcaatgggga tcaatgacaa ctgcacccgg
      841 cttcatcctc cggttcaagg ataaattctt gtaccaaaaa cagcactccc caccctgatg
      901 attgttgccc cctcttctat ggcaatttca aggtcgccac tcatgcccat ggagtattcg
      961 cgaatcggtt tttcgggata tttatcattt aatttttcgc ctaattcctt caataatgca
     1021 taggaaggcc gggcgtcttc aatgacctcg gcgttgagcc cgatcgtcat caatccgatt
     1081 agagtaagat tttgcatgtt tcttacctgc tcagctaatt ccggcaaatt ttcaggtgcg
     1141 atgccaaatt tgctcgcttc gaaagacgtg ttgacctgta tcattacttc cacacttcgg
     1201 cccatcgctc ctgctctttt atttacttct tcggcaagtg caagattgtc gatggtctgg
     1261 atacattgaa cgtgtcgaag gacttctttg accttgttgc gctgcaaatg gccgataaag
     1321 tgtctcgtaa tacagagatc ggccaaagca tccgccttgg aagaaaattc ctgaaccttg
     1381 ttttctccga tgagagtggc acctgcttct actgctatgc ggatatcatc aggggaaaca
     1441 gtcttggtgg cgagaaggag attgacatct tcttccgcct gattgcagtc cctgcatgct
     1501 ttcctgatac gctcatggat gacagcaagg ttgtgcctta tttcactttc tcttgtattc
     1561 aatatggcta aatttgattg atattgcaaa gataagtttt gaatgcctga ttaagtgtaa
     1621 ccagccacct caaatcagca ctaattcttc cttataccat ataagttatc agaccttaat
     1681 ttcttcaaaa cgtaaaagtt ccgctaaggt cctttgccgg ccggtctgct gcaacaaaag
     1741 ctttgtgcga ttccctgaga atcttttggt gaacaaaagg agttatttat taatcaggta
     1801 aaagaactaa aacgaatgta atcctttggt gttgagccgt atatccggcc tgccttttct
     1861 gattaaaagc acaggattaa atttacaggt agctgaatag tggcaacaaa tactgattta
     1921 tcagaaaaaa tcaagacctt tgaagtcaaa tggagcaaac tgataaaatt gatgaattcc
     1981 tgaaagtatt ggctgaagca actcaagcct caagttttaa ttcccttacg ctgagcaaac
     2041 cgagggagaa gcacggtaag atcaagagcc tttattttag gccggttatg atcagaaatg
     2101 aggtaaagac gcagatggtc gttcggttca gtgacaggga ggatacgaag aatttctcac
     2161 agactgaatt tttggccttt gcaagccaga gtaccagaca acattttttt tatgcacacc
     2221 tggtcacttc aagtcaagaa gggcatcttt tgatctccag acgtggcttt gtcaccttga
     2281 agtggaagca actgaccagg gaaatgattg cactccgggt tcatgataaa gaaaaacaat
     2341 acatcattcc gccggaagaa ccctttttgc aacatcttgg tatcagctcc tcaacaggta
     2401 caattatcaa ggatcagtat aagaagtatc gccagataag ccgttacata gagctttttg
     2461 ctcctctcac ggaaggtttg gataagactg tgcctctgga agttactgat atgggttgcg
     2521 gtaaggggta tcttaccttc gcaatgcatc gctttttgaa aggggcggga ttcaagcagg
     2581 tacacacatc aggtatcgat atcaagtcgg atgtcatccg aaataacaac atcatcgccc
     2641 gggaagtagg ctacgatggt ctggagtttt tgcacggccg aatcgaagag aacagggata
     2701 ttaatcctga catcctgatc gccctgcatg cctgcgatac agctaccgat gacgcaattt
     2761 tttatggagt cgaacgaaaa gccaaagtca ttgttgtagc tccttgttgt cataagcagg
     2821 tcaggaaatc aatggcacgc aatgaactta cctcgggtct attccgtcat ggtatcatcc
     2881 aggaacgatg ggccagcgac ctgacagaca tgatcagggc aaatatactt cgctatctgg
     2941 ggtatcaggt caagattatg gaattcgtga gtatcgaaca tactccgaaa aatatcatga
     3001 ttaccgccca atattatggc aagattaatg aaacagccag acaagagata tatacctgga
     3061 tggagatgtt tggtataggt gagcagtatc tattgaataa actcggcctg gctcccgaca
     3121 attgaatgga aagatggtgt tgtaaaaggc attcttacct gtttttttaa aaaaacatga
     3181 ttctaatcca atctgcccca actgaaagaa gagtaatatc tcgacggtct tatgttttac
     3241 tctattgctt tgtatatatt ttgaagaaat aaggaatcca tgtcaccata caataagata
     3301 tttgttacga gattgagcta tttgacaggt aatcagaatc ttagtaactg gtcgcgatga
     3361 tcgattttcg gcatgttcca agtgaataaa ggtaggatcc ttagcgagta agctgattgt
     3421 gtattccgga ataatgagga gatcaaatcc gggagaaggt tgaaatttta tatcatcatg
     3481 atttctgatt aagagatttt cttttttaat cttacatgcc gttatagtta gcccgatttt
     3541 ttgtctgatg gtgcattcct agcaagaatc taaattgttt ctgcatagcc ggtcagttgt
     3601 ttattgactt cttcctggtt aagaatagta attattttat acatttatta ttgaattcaa
     3661 taggtattat gtgcagtttc gaaataaaac attttcaatc cattacagta aaaagttttt
     3721 tgtttacctt aatcgttgca ttttcgtccg ttcagtcagg agtttctcaa aatccttttc
     3781 cggtatggcc ggattccata ttttccgctt attatcatca gcgtgccgga ctttttaatg
     3841 tgatgagcgg cactaaggga ggcgtgtact ttgtcggtaa cagtatcacg gatggtgccg
     3901 aatggagtga aatattcggt gatgcaaaca tcagaaatcg cggcatcagt gcagacatct
     3961 ccgccggagt gctcaatcgc attcaaacat tgatcgaaag aagaccggat aaggtctttc
     4021 taatgattgg aatcaatgac ctcgcacgcg gaattgcacc ggatagtatt ctgcacaata
     4081 ttggcacctt tgttcgcctt ttgcgggaat atagtccgac gacacagata tttatccaaa
     4141 gtttactgcc cgtcaatccc gacctgaaga tgtttgcaca acacacatcg aaggcagcag
     4201 agattaaata tgtcaatggc atactggaga gggaggccgg acagggttat gcctacatag
     4261 acctgcacac tcactttctg gatgaggatg gtaagttgtc gaggcgatgg accaatgatg
     4321 gcctgcattt gaatggtgat ggctatatgt tatggaagca tattgtgttt cccttggttt
     4381 atgacctcca ggctcacccc gccattattc ctcaaccttt ggatatctcg tggctggaag
     4441 gttcttttcc attgtacaaa ttgaaaaata ttactattcc ccggtccggg cttgataacg
     4501 aagctgtcat tctaaaggat tttttgggca gtcttggact atacccccga ataataccga
     4561 caggcagccc ggaagggccg gtcattgtgt taaacctcga tcctgcttat gatggccctt
     4621 ctcaggaagc atatagcatc atggtcagtc cacagagggt tgagatacgg gcaaaaggtg
     4681 cacatggcat cttacatggt attcagacct taaagcaact ctgcagagat ggtgtcatgg
     4741 ttccctgttg tatcattaag gatgcaccgg ctttccaatg gcgaggcttt atgatagatg
     4801 ttggccggaa ctatgtttct cctgacattt tgaaacagat tatcgacaaa atgagttttt
     4861 ataagtttaa catatttcat tttcatccta cggaagacat cgcttggcgg ttggaaagca
     4921 ggctttatcc acaacttacc gcaccggagt atatgctgcg tgataagggc ctgtattata
     4981 ctgtcgccga agtaaagtca ctgatcgagt tttgcagggc acgacacatt accttcgtgc
     5041 cggagataga tatgccgggg cacagtgccg cttttcgcag agcgatgggt gtggatatgc
     5101 agagcgattc cggtacggtg ttggtaaaga agatcattgg tg
//
LOCUS       DAOQMC010000166         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7541_length_5094_cov_5.562215, whole genome shotgun sequence.
ACCESSION   DAOQMC010000166 DAOQMC010000000
VERSION     DAOQMC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7541_length_5094_cov_5.562215"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKV31_07295"
     CDS             <1..856
                     /locus_tag="PKV31_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNB92700.1"
                     /translation="TMNQIIRAIEFAATQPHINVVSTKLTGLGRFGLLEKMHNKEELT
                     DEENEEFNNLLKRIDSICYNANNHGISVFIDAEETWIQDPVDEIADKMMARYNKTRPV
                     VYNTFQMYRKASLGFLKQSYEKAQKEGYILGAKLVRGAYMEKERKRAADMGYPSPIHD
                     SKADTDTDFDDGITYCIENISGIASCCASHNQKSSEIYARLLIDNNIEGNHPHVMTSQ
                     LYGMSDHLTFNLADAGIHSTKFMPYGPVKEVMPYLMRRAQENTSVTGDMSREFELIDR
                     EMKRRKRK"
     gene            complement(863..2014)
                     /locus_tag="PKV31_07300"
     CDS             complement(863..2014)
                     /locus_tag="PKV31_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNB92701.1"
                     /translation="MVTFSNRLIEIRRHLHKHPELSFQEKETAAYIEQRLIELGVEYT
                     IGWAGYGIVATIKAPVATDHFIALRADIDALPIQETGTMEYASQHPGVMHACGHDVHT
                     TCLLGAIELLIANRERLASDVVCVFQPGEELLPGGASLMMKEGAFDKGLCKAIFALHV
                     FPSMEVGKVGFRAGPYMASSDEIYIRVIGQGGHGAMPHHTADPILAAAGLITSLQSIV
                     SRNCDPVLPCVLTIGKINSEGGATNVIPDSVSLEGTFRTMDENWRSRAHELIGKHCRS
                     IEESFGVRIELNIIKGYPVLVNDEIATQKARSVAVNILGEENVEVLPVRMTSEDFAWY
                     TQEIPGCFFRLGTGNKSRGITSNVHTSTFDVDEDCLALGARLMAEICLQ"
     gene            complement(2036..3244)
                     /locus_tag="PKV31_07305"
     CDS             complement(2036..3244)
                     /locus_tag="PKV31_07305"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNB92702.1"
                     /translation="MFDNADLSNKKVVVRVDFNVPLDSNLNVTDDTRIRAAIPTIREL
                     HHRGARVILMSHLGRPLKDLNEDGSIKTKYSLKNIVSSLSDSLNLDVIFIATSVQDNA
                     ILTDIENTGDNRIILLENTRFYKGEEKGDPELARKMAALGDYYVNDAFGTAHREHAST
                     ATIARYFDKDHKSFGFLMDKEVSNISRVLDDIQKPFCAILGGAKVSDKIKLIENLLDK
                     ADKIIIGGGMAFTFIKAQGGTIGKSLCEDDKLDLANQLLAKAGEKGVELLLPVDAKVS
                     ETFSNDGLFVTKPIDDIPDGWMGLDIGEASISLFEEAILSSKMILWNGPMGVFEFSNF
                     AHGTFEIASALAKVTKNGAFTLVGGGDSVAAISKAGLDDSVSFVSTGGGAMLEMIEGQ
                     VLPGVAAMQA"
     gene            complement(3365..>5094)
                     /locus_tag="PKV31_07310"
     CDS             complement(3365..>5094)
                     /locus_tag="PKV31_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB92703.1"
                     /translation="KKFNLVVLLLAFFGVYTHVDAQSESFKSPGITDEMTVNPDQNRE
                     WRAGQSKYSAKPKNMWEVGVHVGRFSISGDIPTTLFPGWGAGIHLRKAINYALSVRVN
                     AMYGTTKGFDDRFSSASIISLEQLPNVGSITNGMYRNYKNTTITGSIEGVLNIGNILF
                     HKERNKWNLYTAVGVGFASIDTRVNNVKNGAIINWESLNLDPNASYADNVKKLKDAMD
                     SDYETKVGNNRDVSGLFDNGKNALTIPFTVGVSRKLTKRVNLSLEHTYYWADYDAWDG
                     FVYRSAFDQSNDSDNGQYTSLRLGINLGSFDKRTEPLYWLNPLDGALNDVAELKQRPK
                     FDLTDSDGDGVIDMLDQEKNSPSGAAVDTRGVTLDSDKDGIPDYKDDEPYSPPGYEVD
                     SRGVAVIPDKPVTESKLNEILKNTKTDWWLPMIHFDLDKYFVKPEFYPQLQSVASVMK
                     SHPDIKIAVTGYTDVRMGDDYNRVLSYNRAKAAIDYLVGTYNLPRERFVLQYDGKADP
                     IIPDLPASHNITKKQEMQQYINRRVEFRVATAEDAEMAAPTGPKAGADTPGSSRKGSK
                     YSGNSNSGY"
BASE COUNT         1449 a         1222 c          984 g         1439 t
ORIGIN      
        1 caccatgaat caaatcattc gcgccataga atttgcagcc acccagccac acatcaatgt
       61 agttagcact aaattaaccg gattgggcag atttggcttg ttggagaaaa tgcacaacaa
      121 ggaagaattg acagatgagg aaaacgaaga attcaataat ctcctcaaaa ggatagactc
      181 tatctgttac aacgcaaata accacggaat ttcggtattt atcgatgcag aagaaacttg
      241 gattcaggat cctgtcgatg aaatagctga caagatgatg gcacgataca acaagacacg
      301 gcctgtggtc tacaatacct tccagatgta tcgtaaggca agtctgggtt tcttaaagca
      361 atcctatgag aaggctcaaa aagaaggtta tattctggga gcgaagctgg tcagaggtgc
      421 ttatatggaa aaggaaagaa aaagagctgc cgacatgggc tatcccagcc ctattcacga
      481 ttcaaaagca gatactgaca ctgacttcga tgatggcata acctattgca tagaaaatat
      541 aagtggcatt gcatcttgtt gcgcatcaca caaccaaaaa tcatctgaga tttatgcccg
      601 attgttgatt gataacaaca ttgaaggcaa ccatccacat gtcatgacca gtcaactcta
      661 cggcatgagc gatcatctta ccttcaatct ggcggatgca ggtattcatt cgaccaaatt
      721 catgccttac ggtcctgtca aggaagttat gccttacctg atgcggcgag ctcaggaaaa
      781 tacttccgtg accggtgata tgagccgtga gttcgaattg atcgacagag aaatgaaacg
      841 aaggaaacga aaataatttc ttttattgca agcatatttc tgccataagt ctggcaccga
      901 gagcaagaca atcttcatcg acatcaaaag tggaagtatg gacattgctt gttattccac
      961 gtgatttatt accagtcccc aatcgaaaaa aacagccggg tatttcctgt gtgtaccagg
     1021 caaaatcttc acttgtcatc ctgacaggca acacttctac attctcttct cccagtatat
     1081 taactgcaac cgacctcgct ttctgagttg cgatctcgtc attgaccagt acaggataac
     1141 ctttgatgat attcaactcg atccttaccc caaagctttc ttcaatagac ctgcaatgtt
     1201 ttcctataag ttcatgcgcc cgtgaacgcc aattttcatc catcgtcctg aatgtacctt
     1261 cgagagatac cgaatcggga atgacattgg tcgccccacc ctcactattt atcttaccaa
     1321 tagttagaac acagggtaaa accggatcgc agttgcggct tacgatagat tgaagggaag
     1381 ttataagacc tgcagcagct aaaattggat ctgctgtatg atgcggcatg gctccgtgcc
     1441 ctccctggcc gatcaccctg atatagattt catctgaaga agccatatat ggtccggccc
     1501 tgaatccaac cttgccaact tccatactgg ggaaaacgtg gagtgcgaaa atcgccttac
     1561 acaaaccctt atcaaaggca ccttctttca tcatcaatga tgcaccacca ggcaatagtt
     1621 cttccccggg ttgaaataca catacgacat ccgatgcaag cctttctcta tttgcaatta
     1681 gaagttcaat ggcccccaaa aggcatgtgg tatgtacatc atgcccacag gcatgcatca
     1741 cacccggatg ctgacttgca tactccatag ttcctgtttc ctggataggc aaggcgtcaa
     1801 tatctgctct gagtgcgata aaatgatcag tagcaaccgg ggctttgatt gttgctacaa
     1861 taccatatcc ggcccatccg atagtatact caacacctag ttcgataagt ctttgctcaa
     1921 tataagctgc cgtttccttt tcctgaaaag acaactcagg atgcttatgt aggtgccgcc
     1981 tgatctcaat cagcctatta gaaaacgtta ccatgtccat tttcgaaata actgtttatg
     2041 cttgcatggc tgccacaccg ggcaatacct gtccttcaat catctccaac atcgctccgc
     2101 cgcctgtaga gacaaaactg acagaatcat ccaaacctgc tttggaaatg gctgctacac
     2161 tgtctccacc cccaacgagg gtaaatgcac catttttggt taccttggca agtgcagaag
     2221 caatctcgaa ggtgccatga gcaaaattag aaaactcaaa gactcccatc gggccattcc
     2281 agagaatcat cttactgctc aggattgcct cctcaaagag gctgatactt gcttcgccga
     2341 tatccaggcc catccagcca tccggaatat catcgatagg tttagtgacg aacagaccat
     2401 cattgctaaa tgtttcagat actttagcat cgactggaag tagaagttca actcctttct
     2461 cacctgcttt ggcaagtagt tggttcgcga gatccaattt gtcgtcttcg cacaatgatt
     2521 ttccaattgt accgccctgt gccttaataa aggtaaaagc catgcctcca ccaattatta
     2581 tcttatctgc tttatccagc aagttttcta tcagcttaat tttatctgat actttcgcac
     2641 cccccaggat tgcacagaat ggtttttgaa tatcatccaa caccctggaa atattactaa
     2701 cttccttatc catcaaaaat ccgaaagatt tatggtcttt atcgaagtat cgggctatgg
     2761 tagcagtgct ggcgtgctct ctgtgagctg ttccaaaggc atcattaaca tagtagtccc
     2821 ccaaagcggc catcttccgg gccaattcag gatcgccctt ttcctcccct ttataaaacc
     2881 tcgtattctc taacaaaatt atcctgttgt caccggtatt ttcaatatcg gtcaggatag
     2941 cgttatcctg tacagatgta gcaatgaata tcacatcaag attcagacta tcagacaaag
     3001 agcttacaat gtttttaagc gaatactttg tcttgatact tccatcttca ttcaggtctt
     3061 tcaatggtct gccaagatgg ctcatcaaga taacccttgc accccgatgg tgcagctccc
     3121 ggatagtagg tatggcagct ctgattctgg tatcatctgt cacattaaga ttactgtcca
     3181 gagggacatt aaaatcaacc cgtacgacaa ctttcttatt tgatagatct gcattatcga
     3241 acatagttct aacttttttc caaagataat ggcttattct cagaaaaaaa attacctgaa
     3301 aaaatctttg aaaataaaaa agggagccaa atgactccct tttcatcaat atatattttt
     3361 ataattaata cccggaattt gaattgcctg agtattttga tcccttcctg gaagatcccg
     3421 gtgtgtcggc tcctgcttta ggtccggttg gagcagccat ttcagcatcc tcagctgtgg
     3481 ctaccctaaa ttctacacga cggttgatgt attgttgcat ctcctgcttc ttggtaatat
     3541 tgtgagaagc cggaagatcc ggaatgatag gatcagcctt accatcatac tggagtacga
     3601 aacgctctct tggaagattg taagtaccaa ccagatagtc aatggcggct ttagccctgt
     3661 tgtaagacaa taccctgtta taatcatcac ccatccttac atctgtatag ccggtcactg
     3721 ctatttttat atcaggatgt gatttcatca cacttgccac tgactgcaat tgaggataga
     3781 attccggctt gacaaaatat ttgtccaggt caaagtgaat cataggcaac caccagtcgg
     3841 tcttggtatt cttaagtatt tcattcaact tagactctgt aaccggcttg tcaggaatga
     3901 cagcaacacc tcttgaatca acttcataac ccggaggtga gtatggctca tcatccttat
     3961 agtcaggaat accatcttta tcactatcta aggtaacccc acgtgtatcg acagctgccc
     4021 ctgaaggaga attcttctcc tgatccaaca tatctattac accgtctcca tcgctatcag
     4081 taagatcgaa cttaggtcgc tgcttcaatt cagccacatc attcaatgca ccatcaagcg
     4141 gattcaacca gtacagaggt tctgttctct tatcgaaact tccaaggttg attcccaatc
     4201 gtagtgaagt gtattgacca ttatcgctat cgttactctg atcgaaggca gaacgatata
     4261 caaaaccatc ccatgcatca taatcagccc agtagtaagt atgctcaagt gacaagttaa
     4321 ccctcttggt caatttgcgt gaaacaccca ctgtgaaagg tattgtgagg gcgttcttgc
     4381 cattatcgaa tagcccgctt acgtcacgat tgtttccaac ttttgtttca tagtcggaat
     4441 ccatggcgtc ttttaatttc tttacgttgt cagcatagct ggcattggga tccaggttga
     4501 gtgattccca attgatgatg gcaccattct tcacattatt cactctggta tctatagaag
     4561 caaatcccac tcccaccgca gtatagagat tccacttgtt tctctcctta tggaaaagaa
     4621 tgttgccgat attgaggact ccctctatcg atcctgtgat ggttgtattc ttatagttgc
     4681 gatacatacc attggtgatg gatcctacat ttggaagttg ttccaaacta atgatggagg
     4741 cagaagagaa cctgtcatcg aatcctttag tagttccata cattgcgttt actcggacag
     4801 aaagcgcgta attaatcgct tttctgagat gaataccggc gccccagcct ggaaagagag
     4861 tagtaggaat atctccgcta atcgaaaaac gacctacgtg gactccaact tcccacatat
     4921 tcttaggctt agctgaatat ttactctgcc cggctctcca ttccctattc tggtctggat
     4981 tgactgtcat ctcatcagtt atccccggag acttgaatga ttcactctga gcatctacat
     5041 gtgtataaac gccaaagaaa gccaacagta ataccactag gttaaatttc ttgg
//
LOCUS       DAOQMC010000167         5084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7571_length_5084_cov_4.179956, whole genome shotgun sequence.
ACCESSION   DAOQMC010000167 DAOQMC010000000
VERSION     DAOQMC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_5084_cov_4.179956"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="PKV31_07315"
     CDS             <1..1540
                     /locus_tag="PKV31_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNB92704.1"
                     /translation="SSIGKAAYDGGPGVVYLYIFTAIACGFAALCYAEFASTVPVSGS
                     AYTYSYVAFGELFAWIIGWDLLMEYAIGNIAVAISWSDYFTNLVGKAGLHIPDWLTMD
                     YVTAHAGFEKVNAALGTGANISSMDVVELSKYYAWTNAPSIGPLRFIIDLPAMAIVAL
                     ITYIVYVGIRESRTASNIMVGIKLAIILLVVLIGAFYVNTDNWSPFTPNGIGGILKGV
                     SAVFFAYIGFDAISTTAEECKDPQRDLPRGMIYALIICTVLYVAVSLVLTGMVHYSRL
                     NVGDPLAMVFDAVDMKFVSAIVAGSAIIATASVLLVFQLGQPRIWMSMSRDGLLPKVF
                     SRIHPKYGTPSFSTILTGFVVAVPALFLNLDVVLALTSIGTLFAFVLVCGGILVLQQN
                     PDRPKSKFRVPYINGQWIFPIGLVAAIVAILYNYPTYFHDLLTWENFPMIVFWVVIPV
                     LAFYTIRRKFSLIPILGLVSCFYLMAQESHTNWFRFIIWLVLGLVVYFLYGFTHSKLA
                     KEGK"
     gene            complement(1557..4928)
                     /gene="mfd"
                     /locus_tag="PKV31_07320"
     CDS             complement(1557..4928)
                     /gene="mfd"
                     /locus_tag="PKV31_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNB92705.1"
                     /translation="METIEEIIDRFGSSNPVWPQGDSVKNYYIEGLSGSGPLIFMTSA
                     YRKNPRPFLFVADDKDRAAYLYDTLLTFKPGPVHLLTDSFRRPGRFDEVNAANVLRKS
                     EVSSAISSSVTPLIVVTYPEAIFEKIIKPAELEKDKITVTVGELFDQDFVMNILIEYG
                     FERVDFVYEPGQFSLRGAIVDIFSFGNSQPYRIEMDDDKVESIRVFDPNTQLSVKNVN
                     HLNILPNANVKFESEDRIGLLDLMDTRWHIVLEDRNVLLDKIKAAYEKAEDSLHNASL
                     IEQPEAQKFLRDRAFISPDIFEESLKKYTIFYLSKPDGRIKYETLHFQTKPHPVINKQ
                     FKLLKEHLEENAEKGIKTWFFSENKRQLQRLEAILNDLGAPNTFQPVIGVVHEGLTDE
                     KSKVEILTDHQLFQRYHQYSLKTGYTRDQAVTLRMLKELQPGDYVTHIDHGVGKFSGL
                     ETIDIGGVKQEAVRILYRNNDILYVSINSLHKLSKYVGKDGEPPMINKLGSDSWKQLK
                     QKTKRKVKDIAEGLIKLYAKRKASDGFAFAPDGYLQAELEASFLYEDTPDQFKATQEV
                     KEDMEKPYPMDRLICGDVGFGKTEIAIRAAFKAINDGKQVAVLVPTTILALQHFHTFN
                     NRLRDFSVDVQYLNRFKSTKEKKEVYESLKSGKTDLVIGTHALLNKDVAFKDLGLLII
                     DEEQKFGVAAKEKIRSMMVNVDTLTLTATPIPRTLQFSLMAARDLTVIRTPPLNRQPI
                     YTEIKPFEQRAIREAIENEMRRGGQVFFVHDRVKNLGEMVEFILKLVPDANVAAAHGQ
                     MDSKLLEQTLVDFIEGKFDVLVSTNIIETGLDIPNANTMIINNAHHYGLSDLHQLRGR
                     VGRSNKKAYCYLIAPPMSSLTNEAKKRLKTIVEFSELGSGFEIAMRDLDIRGSGNLLG
                     GEQSGFISEIGYDAFQKILEEAIRELKEEEFKETFREEINTKKVHVRDVVIETDGEML
                     IPNNYVTSTSERFALYHEMDAIEDEEALQKFLLDMADRFGPIPDAVKELADGLRMRWT
                     MRVLGFERGVIKGGHLSLFFPSNPQASYYESETFKAIFQMLATTKNPNLSMQKTLRNL
                     IFKIKGIRSVQDSLHFLTELDGQIDSNRQTMPKSN"
BASE COUNT         1198 a         1099 c         1170 g         1617 t
ORIGIN      
        1 ttcgagcatc ggtaaggcag cctatgacgg cggaccgggt gttgtgtacc tctatatttt
       61 taccgctatt gcctgtggtt ttgctgcctt gtgttatgct gagtttgctt ccacagtgcc
      121 tgtttcaggt agtgcatata cgtattccta tgttgccttt ggggagttat ttgcctggat
      181 cataggttgg gatttgttga tggaatatgc catcggtaat atagcggttg ccataagttg
      241 gagtgattat tttaccaacc tggtcggtaa agccggactt catattccgg attggctgac
      301 gatggactat gtgacagccc atgcgggctt tgagaaagtg aatgcggccc tgggtactgg
      361 cgccaacata tcctctatgg atgttgtaga gttgtcaaaa tattatgcat ggaccaatgc
      421 ccctagtata ggaccactga gattcatcat cgacttgccg gcgatggcga tagtcgctct
      481 gatcacttat attgtttatg ttggaatcag ggagtccagg actgccagca atatcatggt
      541 gggcattaaa ctggcaatca ttctactcgt tgtcctgatc ggtgcctttt atgtcaatac
      601 ggataactgg tcgcctttca ctccgaacgg gattggaggg atattgaagg gtgtgtcagc
      661 tgttttcttt gcttatatcg gttttgatgc catcagtacc acggccgagg aatgtaagga
      721 tcctcaacgg gacttacccc ggggcatgat ctatgctttg atcatctgta ccgtactata
      781 cgttgcagtg tcattggtgc tgaccggaat ggtgcattac agcaggttga atgtcggtga
      841 cccgctggcg atggtttttg atgcagtgga catgaagttt gtctcggcta tcgttgccgg
      901 tagtgccatc atcgccacgg cgagcgtctt gctggtattc cagttgggcc agcctagaat
      961 atggatgagc atgagccggg atggcctttt gccaaaggtt ttctcgcgta ttcatcctaa
     1021 atacggcacg ccttcatttt cgaccatttt gaccggtttt gtagtggcgg tgccggcatt
     1081 attcctcaat cttgacgttg tacttgcctt gacaagcatc ggtaccttgt ttgcctttgt
     1141 ccttgtatgc ggtggtatcc ttgtcttgca acagaacccc gatcgtccga agagtaagtt
     1201 ccgggtccca tatatcaacg gtcagtggat tttcccaatt ggattggtgg cagctattgt
     1261 agccatctta tataattacc ctacttattt ccatgactta ttaacatggg agaattttcc
     1321 gatgattgtc ttttgggtgg taatcccggt gcttgcattc tacaccatta ggcgtaagtt
     1381 ttcgctgatt ccaatacttg gattggtaag ctgtttttat ttaatggctc aggagagtca
     1441 taccaattgg ttcaggttta tcatttggtt ggttctcgga ctggtcgttt attttctgta
     1501 tggatttacc cacagtaagc tggccaaaga aggaaaataa aaattcggat atcgctttag
     1561 tttgatttag gcattgtttg tctgttggaa tctatttgac cgtccaattc tgtcaggaaa
     1621 tgtaaggagt cctggacact gcgaattcct tttatcttga agatcagatt acgcagggtt
     1681 ttctgcatgg aaagatttgg atttttagta gttgccagca tctggaagat ggccttaaaa
     1741 gtttcggact cgtaataaga agcctgtggg ttggatggaa aaaacagact caaatgacct
     1801 ccttttatca caccgcgctc aaagcccaat acacgcatcg tccagcgcat ccttagtcca
     1861 tcggccaact cctttactgc atccggtatt ggtccgaagc ggtctgccat atccaaaagg
     1921 aatttctgta gggcttcttc atcctctata gcatccattt cgtggtataa agcaaatctt
     1981 tcactggtgg atgtgacgta attgtttggt atgagcatct ctccgtcagt ttctatcaca
     2041 acgtcacgga catgtacttt cttggtattg atttcctccc ggaaggtttc tttgaactct
     2101 tcttccttca actcccgaat cgcttcttcc aaaatcttct ggaaggcatc gtaaccgatt
     2161 tcagagatga agccactctg ttcaccacct aagaggttgc cactgcctct gatatcaaga
     2221 tcgcgcatgg ctatttcgaa tccacttccc aattcggaga attcgactat ggttttgaga
     2281 cgtttttttg cttcattggt aagggaggac atgggtggag ctattaggta gcaatatgcc
     2341 tttttgttgg agcgaccaac ccttccccta agctggtgga ggtcgctcaa gccgtaatga
     2401 tgcgcattgt tgatgatcat cgtattggcg tttggaatat ccagtccggt ttcgatgata
     2461 ttcgtcgata ccaggacatc gaactttcct tcaataaaat cgacaagagt ttgttccaga
     2521 agtttgctgt ccatctgccc atgtgctgca gcgacattgg catccggaac taatttcagt
     2581 ataaattcca ccatttcccc cagatttttc acccggtcat ggacaaagaa cacctggccg
     2641 ccacgtctca tctcgttttc gatagcctcc cggatggctc gttgttcgaa aggctttatt
     2701 tctgtgtata ttggttgtct gtttaatgga ggtgtacgta tgacggttag gtcccgtgcg
     2761 gccattaggg aaaattgaag tgtacggggt atgggtgtgg cggtcaaggt gagcgtatcg
     2821 acattaacca tcatggatcg aattttttct tttgcagcca ctccaaattt ttgctcctca
     2881 tcaattatca gtaagccgag gtctttaaac gctacatcct tgttcaataa agcatgtgtg
     2941 cctatgacga ggtcagtttt cccggatttc agactttcat atacctcttt tttttcctta
     3001 gttgatttga aacggttgag gtattgaaca tcgacactaa aatccctgag tctgttgtta
     3061 aatgtatgaa aatgctgtag tgcaagaatg gtggtcggta ccagcactgc tacttgcttg
     3121 ccgtcattga tggctttgaa tgcggcacga atggctattt ctgtttttcc aaaaccaacg
     3181 tctccacaga tcagtcgatc cataggataa ggtttctcca tatcctcctt gacttcctgc
     3241 gtagccttga attgatccgg agtatcttca tacagaaaag acgcttccaa ctccgcctgt
     3301 agatatccat caggagcgaa ggcaaatcca tcgctggctt ttcgcttggc gtataatttg
     3361 atgagtccct cggcgatatc cttaaccttg cgcttagtct tttgtttgag ttgcttccag
     3421 gagtcagaac ccaatttgtt gatcatgggc ggctctccat ccttaccgac gtacttggaa
     3481 agcttatgca gagagttgat actgacatac aggatgtcat tgttgcggta cagtatccgt
     3541 acagcttcct gttttacacc accaatatcg atggtttcca gtccggagaa tttgcctaca
     3601 ccatgatcga tgtgggtgac ataatctccg ggctgcaatt cctttaacat cctcagagta
     3661 acagcctgat cacgcgtata gccggttttt aggctgtatt gatgataacg ttgaaatagc
     3721 tgatggtcag tgagtatttc aactttgctt ttttcgtccg tcagaccttc atgcacgaca
     3781 cctatcacag gctggaaggt atttggtgct cccaggtcat tgaggatggc ttccaggcgc
     3841 tggagttgcc gtttattttc actgaaaaac caggttttga ttcctttttc ggcattttcc
     3901 tccagatgtt ccttcagtaa tttgaattgt ttgttgataa cgggatgtgg cttggtctga
     3961 aaatgaaggg tctcatactt gattcttcca tcaggttttg ataggtagaa aatggtatat
     4021 tttttcaagc tctcttcaaa aatatccgga gagataaaag ccctgtcccg cagaaatttt
     4081 tgtgcttccg gttgctctat cagagatgcg ttatgtagtg aatcttccgc cttttcgtat
     4141 gccgccttta ttttgtcaag caatacatta cgatcttcca acacaatatg ccatcttgtg
     4201 tccatcaggt ccagtaagcc aattctgtct tcggattcga atttgacatt ggcattgggc
     4261 agaatgttga gatgattgac atttttaaca gacaattgtg tgttgggatc aaataccctg
     4321 atgctctcca ccttatcatc atccatttct atcctgtatg gttggctgtt gccaaaggaa
     4381 aatatgtcca caatcgctcc acgcagagag aactgacccg gttcatatac aaagtcaaca
     4441 cgttcgaatc catattctat caggatgttc atcacaaaat cctggtcgaa aagctcacct
     4501 acagtgaccg ttatcttgtc tttttcaagt tcagcaggct tgataatctt ttcaaagatt
     4561 gcttccggat aggtaacaac gatcaaggga gtaacggaag acgagatggc tgaagaaacc
     4621 tcggatttac gtaatacgtt agctgcgttg acttcatcaa agcgacccgg tcgtctgaat
     4681 gaatcagtca aaagatgcac cggtccgggc ttgaaggtca gtaaggtatc gtaaagatag
     4741 gctgcgcggt ctttatcatc agcgacaaac aggaatgggc gagggttttt tcgatatgct
     4801 gatgtcatga atattagcgg cccactcccg ctcaatcctt caatataata atttttaaca
     4861 ctgtcgcctt gcggccatac cggattggat gatccaaacc tgtcaataat ttcttctatg
     4921 gtttccacag tgcaaatgta aaaaaaatga ctgcttatcg ttctctaagt tgagctcaag
     4981 attttaactg tggaattctt agggcagatt tttaatgtgc gaatatatgt atgccgttgc
     5041 ctgctgcatt taacctggat ttattggctt taaagaggtt tttg
//
LOCUS       DAOQMC010000168         5074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7597_length_5074_cov_5.535565, whole genome shotgun sequence.
ACCESSION   DAOQMC010000168 DAOQMC010000000
VERSION     DAOQMC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7597_length_5074_cov_5.535565"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKV31_07325"
     CDS             complement(<1..339)
                     /locus_tag="PKV31_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92706.1"
                     /translation="MNKKISEFVAFSIFRLFGIIVVGILCSILGFIIYNGIKVINWEF
                     LTTFPKEGMTSGGIFPAIVGTCYLIIGSMLFAFPLGVMSAIYTNEYGGQSWIVRLIRM
                     MTNNLASIPSI"
     gene            complement(342..557)
                     /locus_tag="PKV31_07330"
     CDS             complement(342..557)
                     /locus_tag="PKV31_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92707.1"
                     /translation="MVTGNAAIIPQSLFDPVRTIPATIAAELGEAPTGSAHYQALFLL
                     GCILFILTMAISVIAELISKKKSYKVH"
     gene            complement(514..1554)
                     /locus_tag="PKV31_07335"
     CDS             complement(514..1554)
                     /locus_tag="PKV31_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB92708.1"
                     /translation="MAEKKFYKRLLEKTIEQLLRISGTVTSITILFITLFLFKEGINL
                     FNTPDIEDGYGLYVHVANQKEQLSPEEIKEIFDSEITNWNQLGWKDAEIKIFRIDDVF
                     EIYPESEMGENYALLPGKLAEIISKEENIIAFLPKQFAPNAPEVKELKTGNIRWQDYF
                     LGKEWLPTASPSPLFGALPLIAGTLFVSIIALLIALPLGLGVAIYLSELAGEGQRRIL
                     KPVIELLSGIPSVVYGFFGLVVLAPLVQKIFNLPVGETALTGSLILAIMALPTIISVS
                     EDALRNTPREMREASLALGASRWQTIYKVDTLCQNRYFCCCRIGNRKGYRRNNGSING
                     YRKCCHHPSIPL"
     gene            complement(1578..2393)
                     /locus_tag="PKV31_07340"
     CDS             complement(1578..2393)
                     /locus_tag="PKV31_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNB92709.1"
                     /translation="MKRIITILLAILGVTFAADAQKIKGSETVLPISEKAAQVFMKSN
                     NSGNVTVTGGGSGVGISSLIGRSADIAQTSRKMKFSEKQKFQRSGKAVKEVIIAYDGL
                     AVIINPANKIKKLTREQLEDIFTGKIKNWKDVGGADMKVIPYARETSSGTYEFFKEVV
                     LKNKNYLSGIMSMPANGSLIQSVSQTKGAIAYVGLAYVSASVKTISVSFDKGKSYVFP
                     SETSVKNKTYPVVRPLYYYYLTKSEFLVKKFIDFILSPAGQKIVREVGYISVN"
     gene            complement(2934..4406)
                     /locus_tag="PKV31_07345"
     CDS             complement(2934..4406)
                     /locus_tag="PKV31_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB92710.1"
                     /translation="MTDSLAAGLFRPSRLLLLINLFLACCIILAIYFDQYWLIALPAV
                     FLLGFVLLRDLRFLYIIMMMSVPISIEYSIGSFTLDAPDELLNIFFLALMPGFLLFNY
                     KNINFSFVRHPIVLFLAASFLLSIITTIFSVNVFLSVKYLLAKAWYIVAYFGMTALFI
                     KSWKDVKTLAIAVGFTAVLTMVYVMSRHAVRGFSFDSINSCVGPFYSNHVNYAIQLLV
                     IFPLIWLLGRIHQLKWKQNYLYIALMLLFIAGIYFSYTRAAIGALLLLIPFYYIVKWK
                     LMKPAIILSIIGVFSYSIYLIKDNKFFDYAPDYNKTISHTDFNTLLTATYNFEDISTM
                     ERVYRWVAARYMIADRPMIGVGPNNFYDTYSAYTLKSFRTYVSDNPDHSTVHSYFMLL
                     AIEQGILAPILFVWMVAWAMIAGQRMYHRETDKRNQYVIMAAMMILFAMLVVLTINDV
                     IESDKEGSFFYVALALIMRFHWKSIKGTDNPTSPETGKAQ"
     gene            complement(4399..>5074)
                     /locus_tag="PKV31_07350"
     CDS             complement(4399..>5074)
                     /locus_tag="PKV31_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92711.1"
                     /translation="SMANAARYFINELVSNLLKTNLVELSLTIKDNIAQKQKEVEILS
                     DSVRALRNKYKIIEPDAQGKELMQKMVNVETKFKREQERLKVLETLPGIRRDTINLLK
                     AMVAGLKEEYKSITDSNTTSAINFNNFNEGRNIIEVMSTQLRSAKDYLSVYNTRYNQL
                     IGVLKSKNNALHIVEKADIPLVKSRPKRSMIVLACLIAALMVNVLGIIIVDSLRDPQW
                     KKLWND"
BASE COUNT         1504 a         1037 c         1026 g         1507 t
ORIGIN      
        1 aatagagggt atgcttgcca gattgttggt catcatcctg atgagtctga ctatccagct
       61 ttgcccgcca tattcgttgg tataaatggc agacatgact cccaggggaa aggcaaacag
      121 catgctgccg atgatcagat aacaggtacc tacaatagcc gggaagatac cgcctgaggt
      181 cataccttct ttggggaagg ttgtgagaaa ttcccagttg attaccttaa ttccattata
      241 gataataaat cccagaatac tgcacagaat gcctaccaca atgataccga atagtctgaa
      301 tatggaaaaa gctacaaatt cacttatttt tttattcata actaatgtac tttataactt
      361 ttcttttttg aaattaattc agctataacc gatattgcca ttgtgagaat aaatagaata
      421 cagccaagca ggaacaatgc ctgataatgt gcgcttcctg ttggagcttc tccgagttct
      481 gctgcaatgg tagcaggtat tgtccgcaca gggtcaaaga gggattgagg gatgatggca
      541 gcatttcctg taaccattaa tactgccatt gtttcgccga tagcccttcc gattcccaat
      601 acgacagcag cagaaatacc tgttttggca taaggtatcc actttgtaga tggtctgcca
      661 tcggctggca cccagagcca ggcttgcctc acgcatttcc cggggcgtgt ttcgcaaagc
      721 atcttcagaa actgaaataa tggttggcaa cgccatgatc gccaggataa ggcttcctgt
      781 cagtgcggtt tctcctactg gaagattgaa tatcttctgc acgaggggtg ccagcacaac
      841 caatccgaag aacccataca ccaccgatgg gatccccgat aatagttcaa ttacaggctt
      901 caatatccga cgctgtcctt caccggcgag ttccgaaaga tagatggcga ctcccagacc
      961 cagcggcaat gctatcagca atgctataat gcttacaaac aatgttccgg ctatcagtgg
     1021 caatgcacca aaaagtggag aaggagaggc tgttggcaac cattcttttc caaggaaata
     1081 atcctgccat cgaatgttac cagttttgag ctccttcact tcaggcgcat ttggtgcaaa
     1141 ttgtttgggc aggaaagcga tgatattttc ttctttgctg atgatctctg ccagcttgcc
     1201 gggtaataaa gcgtaattct cccccatttc actttcagga taaatctcaa atacatcatc
     1261 aatcctgaat attttaatct ctgcatcttt ccatcccaat tggttccagt tggtgatttc
     1321 ggaatcgaaa atttccttta tctcttctgg actcagttgt tccttctggt tcgctacatg
     1381 cacataaagc ccgtatccat cttctatatc gggagtgttg aacaaattta ttccctcttt
     1441 aaaaagaaat aaggtaataa acaggattgt gatacttgta acggtaccac tgatccttaa
     1501 taactgttca attgtttttt ctaaaaggcg cttataaaat ttcttttcag ccatcactta
     1561 gcattagttc gaaactatca atttacactg atgtaaccga cttccctgac tatcttctga
     1621 ccagccgggg aaagtatgaa atcgataaat tttttcacca ggaattccga tttagtcaga
     1681 taatagtaat acaatggtct gacaacagga taggtcttgt tttttacact tgtttcggag
     1741 ggaaagacat acgacttgcc cttgtcaaag gacacagaga ttgtctttac agacgcactg
     1801 acataagcca ggccgacgta ggctatagct cctttggttt ggcttacgga ttgtatgagg
     1861 gagccattgg ccggcatgct cataattccg gacaggtagt ttttgttttt gagcacaact
     1921 tccttgaaaa attcataggt gccggacgaa gtctctctgg catagggaat gaccttcata
     1981 tcagcaccac cgacatcctt ccagtttttg atctttcctg taaatatatc ttccagttgt
     2041 tctcttgtca gttttttgat cttgttggcc ggattgatga ttacagcaag tccatcatag
     2101 gcgattataa cttctttgac agccttgcca cttctctgga atttttgttt ttctgaaaat
     2161 ttcatttttc gggatgtctg agcaatatcc gccgatctcc caatcaagga tgaaattccc
     2221 acaccgctgc ccccacctgt aacagtcaca ttacctgaat tatttgattt catgaaaacc
     2281 tgagctgctt tttcagaaat cgggaggacg gtctcggaac cttttatttt ctgagcatcg
     2341 gcagcaaaag tcacacccaa aatggccaat aaaattgtga taatcctttt catttattta
     2401 atttgcgcaa aattgaggca ccgatgtttc aatacatttg agataatatt acaatttggt
     2461 taagacttca gaatttttag gcagacaaag cataaatact ttatctctca ttttttcact
     2521 taagaggcag gaggggattt tattagtaat tattatgttt tatattatcg gctttaatac
     2581 cttataccaa gatgatttat ataatattcc ataaattttt ggtatacttg tcccgagagc
     2641 taagcgattc ggggatgccg aggttacagt cgtataaatc aatgaaattg gtctataacg
     2701 aatgtacctt cggtattagg ttgcattaat cattgcagat tatcagccgg aatcacctgt
     2761 gtgtgattaa ccaatcgagt gacaattgac cagtttcaaa tactattgtt tatccaggaa
     2821 tatccaaaat attttagtga aaatcaacca atataataat ttatatataa aattattata
     2881 tacaaataca gctaataatc aaaattatat caatctatta aagggctttg aatctattgg
     2941 gcttttcctg tttcagggga tgtcgggttg tctgtccctt ttatgctttt ccaatggaat
     3001 ctcataatca atgccagtgc aacataaaaa aaggaacctt ctttatcgga ctcgatgaca
     3061 tcattgatcg ttaaaacgac cagcatagca aaaagaatca tcatagcagc catgatgaca
     3121 tattggttcc ttttgtctgt ctctcgatgg tacatacgtt gtcctgcaat cattgcccag
     3181 gcaaccatcc agacaaatag gatgggagcc aggattccct gttctattgc cagcaacata
     3241 aaataagagt gtaccgtgct gtgatcggga ttgtcactga cataagtcct gaaggatttg
     3301 agggtgtaag cagaataagt gtcataaaaa ttgttgggac caactcctat cataggtctg
     3361 tcggctatca tatatcttgc ggcgacccaa cgatacaccc gctccatggt ggagatatct
     3421 tcaaagttgt atgtcgcagt aagtaatgtg ttgaaatccg tatgagagat tgttttgttg
     3481 tagtcgggcg cataatcgaa aaatttattg tccttaatca gatagattga gtatgaaaaa
     3541 actccgatga tggaaaggat gatagccggc ttcatcagct tccacttgac aatataataa
     3601 aagggaataa gcagcaataa agcaccgatg gcggcccgtg tgtaactaaa gtatatcccc
     3661 gcaataaaca gtaacatcaa ggcgatgtat agataatttt gcttccattt caattggtgt
     3721 attctgccaa gcaaccatat caacgggaaa ataacgagca attggattgc atagttgaca
     3781 tgattggaat aaaaaggtcc gacacatgag ttgatggaat caaaggaaaa gcctcgtact
     3841 gcatgtcttg acatgacata aaccatggtc agcacagcag taaatccgac ggcaatagcc
     3901 agcgttttga catctttcca ggactttatg aaaagtgcgg tcatgccaaa ataggctaca
     3961 atataccagg cttttgcgag gagatacttc actgaaagaa aaacattgac agagaagatt
     4021 gtcgttatga tggataaaag aaaagatgct gccagaaaaa gaactatcgg atggcgaacg
     4081 aagctgaaat tgatattttt gtaattgaac aacaagaatc caggcatcaa ggccaggaaa
     4141 aaaatattga gcaattcatc cggtgcatcc agtgtaaagc taccaatgga atattcaatg
     4201 gatattggta cgctcatcat catgatgata tacaggaacc tcaggtctct tagcagaacg
     4261 aatccgagca gaaaaactgc cggtagggca atgagccaat attgatcaaa gtaaatagca
     4321 agaatgatac agcatgccaa aaacaggttg atcagcaata acaggcgcga tggcctgaaa
     4381 aggccggcgg ctaaggaatc agtcattcca gagttttttc cattgtgggt cacgcaggga
     4441 atcgacgatg atgataccca acacattgac catcagtgca gcaatcaagc atgccagaac
     4501 aatcatagat cttttcggtc ttgacttgac caatggaata tcagccttct cgactatatg
     4561 caatgcatta ttcttactct tcagaacgcc gatcaattga ttatatctgg tattatatac
     4621 agacagatag tccttggctg acctcaattg tgtgctcatc acctcgatga tattacgccc
     4681 ttcgttgaaa ttattgaagt tgattgcact ggtagtgttg gaatcagtaa tggatttgta
     4741 ttcttccttt agacctgcaa ccatagcttt gagcaggttg atagtgtcac ggcggatacc
     4801 gggcagggtc tccaatacct taagccgctc ctgttcccgt ttgaatttgg tttctacatt
     4861 gaccattttt tgcattaatt ccttaccctg agcatcaggt tcgatgattt tatatttatt
     4921 ccttaacgcc cttacggaat cagaaagaat ttctacttct ttttgttttt gcgcaatatt
     4981 gtccttaatt gttaaagaga gttctacaag attggttttt aagaggtttg aaaccaattc
     5041 attgataaaa taccgggctg cgttggccat tgag
//
LOCUS       DAOQMC010000169         4986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7817_length_4986_cov_4.189008, whole genome shotgun sequence.
ACCESSION   DAOQMC010000169 DAOQMC010000000
VERSION     DAOQMC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7817_length_4986_cov_4.189008"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKV31_07355"
     CDS             complement(<1..286)
                     /locus_tag="PKV31_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92712.1"
                     /translation="MKLLEGKTAIITGGSRGIGAAIVRLFAEHGAEIAFTYASSAGPS
                     EALVAELSAKTKIKAYQSDAASMSAAEQLVKDVLGDFGKIDVLVNNAGITK"
     gene            complement(288..1298)
                     /locus_tag="PKV31_07360"
     CDS             complement(288..1298)
                     /locus_tag="PKV31_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92713.1"
                     /translation="MKRYNYWLVAAFCLLFAGSSTAQKRMALVEHFTNSKCPICKANN
                     PGFYSRFEKYSNDAIHIAYHPSVPYNSCIFYVANKDGNGARQSFYEIIGTPTAFVNGR
                     KGTSSLPSDADYLNAINQPAEYDITITSAAPGNVELKITPLKSLPSGAQLRLFTALIE
                     NKVLYDAPNGETEHPNVFRKFLGDQNQGIVVSFPEGTAANSSKSVSLGAIDYSGVTDW
                     SKFSIIAFINLDGQKEILQAAKQSVGALSETNVVEHIPTCIFPNPSRDAVRISWADTA
                     PRDIEIKIFDMQGNLRSVIKPDAGRSFIEINTSGHIPGTYIYQMIGPEKKLLSNGKFI
                     LQ"
     gene            complement(1447..2400)
                     /gene="ftsY"
                     /locus_tag="PKV31_07365"
     CDS             complement(1447..2400)
                     /gene="ftsY"
                     /locus_tag="PKV31_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNB92714.1"
                     /translation="MSFFNKFFSKDKEKDLDNSLSKTKESFFTKITKAVAGKDKVDEE
                     VLDEVESILISSDVGLDTTVKIIERLEARVARDKYLNSSELDEILKQEIMQLLADNNT
                     VELEDFDLPDVRPYVIFVVGVNGVGKTTTIGKLAYQFKQKGHKVVLGAGDTFRAAAVD
                     QLKIWADRVGCDFYSKGMNADPAAVAYEAVDYAIKNQCDVCIIDTAGRLHTKVNLMNE
                     LAKIKKSAGKRMDGAPHDVLLVLDATTGQNAIEQATHFTQSSDVTALALTKLDGTAKG
                     GVAIGISDQFKIPIRYLGVGEGIDQLQVFNKRAFVESLFKK"
     gene            complement(2540..2710)
                     /locus_tag="PKV31_07370"
     CDS             complement(2540..2710)
                     /locus_tag="PKV31_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HNB92715.1"
                     /translation="MAKKVSKNAKIAQRAANVGSGRDHVKVIKSIKDPVTGVYSYKEA
                     IVHKDKVKDFFN"
     gene            complement(2713..2901)
                     /gene="rpmG"
                     /locus_tag="PKV31_07375"
     CDS             complement(2713..2901)
                     /gene="rpmG"
                     /locus_tag="PKV31_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNB92716.1"
                     /translation="MAKKAKGNRIQIILECTEHKASGMPGTSRYVTQKNRKNTPDRME
                     LKKYNPILKKMTVHKEIK"
     gene            complement(2922..3170)
                     /gene="rpmB"
                     /locus_tag="PKV31_07380"
     CDS             complement(2922..3170)
                     /gene="rpmB"
                     /locus_tag="PKV31_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNB92717.1"
                     /translation="MSKVCQLTGKRPITGNNVSHSNRKTRRRFLPNLQKKKFYIPEED
                     VWITLKVSTSAIRSINKMGITSYLKKLGNTGDSINIYY"
     gene            complement(3208..3281)
                     /locus_tag="PKV31_07385"
     tRNA            complement(3208..3281)
                     /locus_tag="PKV31_07385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3245..3247),aa:Arg,seq:acg)
     gene            3894..4856
                     /locus_tag="PKV31_07390"
     CDS             3894..4856
                     /locus_tag="PKV31_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNB92718.1"
                     /translation="MIRILANDGIDADGKMLLEEAEYIVDTDKVAQEDLPKVLPKYDV
                     IIVRSATKVRKELIDACPNLKVIARGGVGMDNIDVDYARSKGIAVINTPGASTRSVAE
                     LTMAHIYALSRHIHHAKKELSSEKGDFKTLKSIYSKGSELAGKTLGIIGFGRIGQEVA
                     KLAMGANMRILPYDPFVEETVLNFDIFGNENLTLAVKIHTIEKDYLLRHSDFITLHLP
                     YSDGKPIIGIEEIAAMKDGAMLINSARGGAIDENALLDGLNSGKIHGAGLDVFENEPN
                     PRKDLIEHPLVSVTPHIGGSTKEAQAKIGIELADRIITFFESRG"
BASE COUNT         1386 a         1076 c         1036 g         1488 t
ORIGIN      
        1 ctttggttat tccggcatta ttgaccagga catcgatctt tccaaaatca cctaatacat
       61 ccttgacaag ttgttccgca gccgacattg atgcagcatc cgactgatat gccttgattt
      121 tggtctttgc agacaattca gcaaccaatg cctcggacgg gcctgcagat gaagcatagg
      181 taaatgcaat ctccgcacca tgctcagcaa aaagtctgac aatcgctgct ccgataccgc
      241 gggaaccacc cgttataatg gctgtttttc cttccaataa tttcatgtta ctgtaaaata
      301 aattttccgt ttgataataa ctttttttcc gggccaatca tttggtaaat ataagtgccc
      361 ggtatatgac ctgaggtatt tatttcgata aaacttcttc ctgcatccgg ttttataacc
      421 gatcggagat tgccctgcat gtcaaatatt ttgatttcaa tgtctcgtgg tgcagtgtcc
      481 gcccaactta tccggactgc atccctggat ggattgggaa aaatgcaggt cggaatatgc
      541 tcaactacat ttgtttccga caatgccccc accgattgtt tagcagcctg gaggatctcc
      601 ttctgaccat cgagattaat gaaggcgatg attgaaaact tcgaccaatc ggtcactccg
      661 gaataatcaa ttgctcctaa agagactgac tttgaggaat tggcagcggt tccttcggga
      721 aaactcacca cgatgccctg attctgatct ccgagaaatt tcctgaaaac gttcgggtgt
      781 tctgtctccc cattcggggc gtcatacaac actttgtttt cgatcagtgc ggtaaatagc
      841 ctcaactgtg caccggatgg caaggatttg aggggcgtaa tcttcagttc gacattgccc
      901 ggtgcggcag aagtaatcgt aatatcgtac tctgcaggtt ggttgatagc attcagataa
      961 tcggcatctg aggggagcga agatgtgccc ttcctgccat tgacaaaggc tgttggcgta
     1021 ccgatgattt cataaaagga ttgtcgtgct ccgtttccat ccttgttggc cacataaaag
     1081 atgcaactat tgtatggaac tgatggatgg taagcgatat ggatagcgtc attgctgtat
     1141 ttctcaaaac ggctatagaa accgggatta ttcgctttac agatcgggca tttactattg
     1201 gtaaaatgct ccaccaacgc cattcttttt tgggctgtgg atgaaccggc aaatagcaag
     1261 cagaacgctg caacaagcca ataattatat cttttcattc ggaaatattt ttggattgac
     1321 ttacaaagat acgtaaagct ttaccaacct caaagttcct taagcaattg accaggaaaa
     1381 acccggcttg ggaagaatat gtcagagaaa aaaaccttac cgaatgagca atcgattgga
     1441 accgccttac tttttgaaaa gggattctac aaatgccctt ttattgaaaa cttgaagctg
     1501 atcaatacct tctcctactc caagatagcg gatcggaatc ttgaattggt ccgagatccc
     1561 gatggctaca ccgccctttg cagtgccatc gagtttggtt aatgcaagag cggtgacatc
     1621 gctgctctgt gtaaagtggg tagcctgttc aatggcattc tgaccggttg ttgcatccaa
     1681 cacaagcagc acgtcatgcg gggctccatc cattcgtttg ccggcagatt ttttgatttt
     1741 agccagttca ttcattaggt tgactttggt atgaaggcgg cctgctgtat caatgataca
     1801 cacatcgcac tgattcttga tcgcataatc gactgcctca taagccactg ccgccgggtc
     1861 ggcattcatt ccctttgaat agaaatcaca gcccacccgg tctgcccaaa tcttcaactg
     1921 gtccactgct gccgcccgga aggtatctcc tgccccaagc accactttat gacctttctg
     1981 cttgaactga taagccagtt tgccgatggt tgttgttttg ccaacaccgt ttacgccgac
     2041 aacgaagatc acataaggcc tgacatccgg caggtcaaag tcttccagct ccactgtatt
     2101 gttatctgca agcagctgca tgatttcttg cttcaggatt tcatccaatt cactgctatt
     2161 aaggtattta tcgcgggcga cacgcgcttc caacctttct atgatcttga cagttgtatc
     2221 caagccaacg tctgagctga tcaggatgga ttcaacttca tcaagcactt cctcatcgac
     2281 cttgtccttt ccggcaactg ccttggttat ctttgtaaaa aaggattctt tggttttaga
     2341 aaggctgtta tctaagtctt tttccttgtc tttagaaaag aatttgttaa aaaagctcat
     2401 tgtattggat taatggattg gtaaaatcag cttttaaagc cgaagccaga caggataatt
     2461 catccggcta aaatacaaaa aggctttttc tacggataga aaaagccaga tatgttttgt
     2521 caaaacaggt cagaaagaat taattgaaaa agtctttaac cttgtctttg tgaacgatcg
     2581 cctccttgta agaatataca ccggtaacag ggtccttgat ggacttgatc accttgacgt
     2641 gatcacggcc tgaacctaca ttcgcagcac gttgtgcaat cttagcgttt tttgatactt
     2701 tcttagccat gattatttaa tttctttatg aacagtcatt ttctttagta tgggattata
     2761 tttcttcaat tccatacggt caggagtatt tttcctgttt ttttgagtca catagcggga
     2821 agttccgggc atgcctgatg ccttgtgctc tgtgcattcc aaaataatct gtattctgtt
     2881 gccttttgct ttctttgcca ttattcgatg attgcagagg attaataata aatattaatg
     2941 gagtcaccgg tattacccag tttcttaaga tatgaagtga tacccatttt attgatggag
     3001 cggatagcac tggttgaaac tttcagcgtt atccatacat cctcttcagg aatatagaac
     3061 ttcttcttct gaagatttgg cagaaatctc ctcctggtct tcctgttgga gtgggataca
     3121 ttatttcccg taattggtct tttccctgta agttgacaaa cttttgacat ggtcttgttc
     3181 tattttgctg tctatctaaa taattcgtga cctcggcagg attcgaacct gcaaccttct
     3241 gatccgtagt cagatgctct atccaattaa gctacgaagc ctctgatttt tttcgaagtg
     3301 caaaggtacg attagttttt cgattaaaaa atagttttag ggataatttt ccaagatttt
     3361 taagtccatc gggaaagggg cagcaatcct aagatcttct actctatttt tacgtttgac
     3421 tttccaacat ccggcacctt gtatgagccg tcattttcca ggaagttatt gaccttgacc
     3481 cacatgccag tcttcacaga ttatacaaag atacctgatt gtgtacgtta attgccggaa
     3541 aaatgaaagt gtcagaccta tcgaaaaaaa tatatatcat tatatatgct tcgtataaaa
     3601 cttattatag ttacataaca atacatcaat gtaatcagat ccctatacag ggaaataata
     3661 gtctttgaag ggcgaagaaa tatctgtact ctatgaccct atacgacgtt atttgacaac
     3721 agtcattatt gtttcaattc gttatctcct gtttttgcaa ccacaccatt cgttattgcc
     3781 ctcatttttc aatgaataca ggaaaaggac aatttgtgat acttacaact attcaaaccg
     3841 gtaccttagt aatgaaagaa atgtttacct ttgcaactca aaaaatttaa agcatgatca
     3901 ggattctcgc aaatgacggt atcgatgccg atggcaaaat gcttcttgag gaagcagaat
     3961 atattgttga caccgacaag gtagctcagg aagatttgcc taaggttttg ccgaaatatg
     4021 atgtcatcat cgtacgcagc gcaacaaaag ttcgcaagga actgatcgat gcgtgcccca
     4081 atttaaaggt aatcgcccgt gggggtgtag gtatggacaa tattgatgtg gactatgcaa
     4141 ggagcaaggg catcgccgtg atcaatacgc cgggagcatc cacccggtct gttgcggaat
     4201 tgacaatggc acatatttat gccctgtcac gccatatcca ccacgcgaaa aaggaactat
     4261 catccgaaaa aggagatttc aagacattaa aatcgattta ttccaagggg agtgagcttg
     4321 caggcaaaac acttggcatt attggattcg gacgaatagg acaggaagta gccaagctcg
     4381 ccatgggagc caatatgcgt attttgcctt atgatccctt tgttgaggaa accgtactta
     4441 actttgatat ttttggaaac gaaaacctga cccttgccgt caaaattcat actattgaaa
     4501 aggattactt gctcaggcac agtgatttta ttactcttca tcttccttat tctgacggaa
     4561 agccaatcat agggattgaa gagattgccg ctatgaagga tggtgcgatg ctcattaaca
     4621 gtgcacgtgg cggggcaatc gatgagaatg ctttgctcga tggtctcaat tccggtaaga
     4681 tacatggagc cggcctggat gtgtttgaaa atgaaccaaa cccaagaaaa gacctaatag
     4741 aacatccatt ggtttccgtg acaccacaca tcggtggttc taccaaggag gctcaggcaa
     4801 aaattgggat tgaattggcg gatcgaatca ttactttttt cgagtcaaga ggctaatttt
     4861 aaaccaataa gggaggtaag ctgttaatgc ctcagaaatt aattaaatca gaaaaccttc
     4921 accctggcca tatgtcacag tccattgaag taatcgatgc ttacgagcat aatctcaagc
     4981 atatag
//
LOCUS       DAOQMC010000170         4930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7956_length_4930_cov_5.127385, whole genome shotgun sequence.
ACCESSION   DAOQMC010000170 DAOQMC010000000
VERSION     DAOQMC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_4930_cov_5.127385"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1010
                     /locus_tag="PKV31_07395"
     CDS             207..1010
                     /locus_tag="PKV31_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB92719.1"
                     /translation="MKRLFVILSALLASMLFWQCKSFLADANNLNAFTVDQDKQLGDQ
                     VAAEIASKPKEYPLLPEKGNEKIYNYIRGLTNSIIQSGKVKYRDQFNWETHIIKDDKT
                     LNAFCTPGGKIYVYTGLIKFLDSEDQLMGVLGHEIAHADCRHSTEQLTKQYGIVFLAD
                     ALLGNKAAIGQIAKTIVGTLAGLTFSRANESEADAKSVEYLCSTSLNAAGAAGFFKKI
                     EGQSQPPTWLSTHPNPANRVANIEKKATDLGCSGKQTFASTYQQMKQLL"
     gene            1068..1772
                     /locus_tag="PKV31_07400"
     CDS             1068..1772
                     /locus_tag="PKV31_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92720.1"
                     /translation="MQRYFSTGFNYLIAFILVMATAISVNAQYSIWSFRAGPSLAFQR
                     WNGLGSTQPLIGYHGIIGMESYEPGKTSSFYGELGYHQRGSSKYVPAYQSNAGINYNS
                     YTIRNVFHNVGLALGGRKSFGPNNKFFYNLALRGEYTIKAQLSDVFVGFSEFVRKFNY
                     GVDVGAGYNFPIGYHMGFVQIQLQPDLSKQIWSTRYSGYDYQGNPVTYPEQKVINYTF
                     ELSLGFRLFGNLIPED"
     gene            1807..2265
                     /gene="tsaE"
                     /locus_tag="PKV31_07405"
     CDS             1807..2265
                     /gene="tsaE"
                     /locus_tag="PKV31_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNB92721.1"
                     /translation="MSNLTSDQFSFIVRDVTRLSQSVNSHLDRLLSNHVILLEAPMGA
                     GKTTFVSALTALLGSLITPSSPTFSICNEYPLASPVSGYTQIVHMDLYRFKSIEEVLT
                     SGIEDYLYDPQSLVVIEWPELITSLVVEPYTYITIEVMDDLQRKFVYSID"
     gene            2368..3597
                     /locus_tag="PKV31_07410"
     CDS             2368..3597
                     /locus_tag="PKV31_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92722.1"
                     /translation="MDTTVLTYRFAPEALPFREVVCYVLKLIQDRLEVVFEENESGQL
                     CFGNGDNEYPLMQSIYEPLSRLKPCEEKYLSGIYIFYEGKMDILASIFFIVHCLGESI
                     SGNTPDQYGRIVFHQSRYGSQHGPNDDLVTPLMREFLLGHFGLDSIKAKVKKQVILSH
                     DIDLLTSGLRRELSFFFRKPSFTLAKALMRHVLTRDKIWGDASSILGPEKDCGYKSVF
                     YFLPEHRTHNGIVNADYDMEALHRYDDRILAAGCEAGLHKSSSDESYSVEKERMGLSV
                     DSNRNHYLRYRLPDDWKRMAEAGFRTDTGLGWSDSPGLRNGFPWGFKPLGTNLTVIPL
                     VLMDTYFDRNDIAEQITETFRSMISGWPDGFQVSVLFHNNYLTPWSNRAFLEQYITLL
                     DFLKKEGIEVVTPSYFS"
     gene            3627..4736
                     /locus_tag="PKV31_07415"
     CDS             3627..4736
                     /locus_tag="PKV31_07415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB92723.1"
                     /translation="MHILMIPAWFQPESVGHHGIFVRDIARALAHCGHTVRIVSFAYG
                     NKYLEVDDGNILEIFHPTAGSGLFSKLSYLKKTGPWLDEYCRRYGKPDIINTHGLSSI
                     RLVSRWAHIHSLPIVHHEHLKALINPGLPFRIRWQARYFYQKVATIIAVSTIHADAIR
                     KLTDTAVQRIPDVIDSAFFNIPLAKNLKTGLQLACISDLDPEKGYELLIEAVGLLAAN
                     NIDFTLHIVGDGPLRSMMERWSEEKGIRHRINLHGRQNRAGVLSILEKCHIYCTATIT
                     ESFGMHIAEALAAGLYVVTTDCGSVHDYISKTNGTIVPQRSAVLLADALQTAITNYSV
                     ENVHAIRASIAEFASAEVVIPQIESVFRSVLRQRR"
BASE COUNT         1327 a         1126 c         1110 g         1367 t
ORIGIN      
        1 cggaaacttc caatctgcgc agaatttcat ccgcataagc gcaacttggg ttaaatcgca
       61 cgccgatgtg acttggccat gaatatgacg tctcaatata tcttatttgc ctggttgtat
      121 tacctttgag gcatcaataa tcaaaaatga cctttgttcg ttttaatgct ttaacttggc
      181 tgaatggctt actataaatc ttgaaaatga aaagactatt cgttatctta agtgccttgc
      241 tggcaagcat gcttttctgg caatgtaaat cctttttggc tgatgccaac aacctgaatg
      301 ctttcacagt ggaccaggat aagcaattgg gtgatcaggt agctgctgag attgcctcaa
      361 aacccaaaga atatccctta cttcccgaaa aaggcaatga aaagatttac aactatatcc
      421 gtggcctgac caattccatc attcaaagtg gtaaggtaaa gtaccgggat cagttcaact
      481 gggagacaca catcatcaag gacgacaaga cactgaatgc attctgtaca ccgggaggaa
      541 aaatatacgt ttataccggt ctgatcaagt ttttggactc tgaagaccag ttgatgggtg
      601 tgctgggtca cgagatcgcc catgccgact gtcgtcacag cacggagcaa ctaaccaagc
      661 aatatggaat tgtctttctt gccgatgctc tgctaggcaa caaagcagcc ataggacaga
      721 tcgcaaaaac tattgtgggc acacttgccg ggctcacttt ctcccgggcc aacgaaagtg
      781 aggcggatgc caagtcggtc gaatatctat gctccacctc ccttaatgcg gccggcgctg
      841 caggcttctt caagaagatt gaaggacaga gtcagccacc tacctggtta tccacccatc
      901 ccaatccggc caacagggtt gccaatatcg agaaaaaagc aacggatctt ggatgtagtg
      961 gcaagcagac ctttgcatcg acttatcagc agatgaagca attgctttga gtcgaaacgc
     1021 ttatatttgc gtacaatccg gattgatcga attaaacgag taagcgtatg caacgatact
     1081 tttctaccgg cttcaattac ctgatcgctt ttatattggt tatggcaacc gccatcagcg
     1141 tcaacgcaca gtattccatc tggtcgtttc gtgccggacc atccctggcc tttcagcgat
     1201 ggaatggcct tggatcgaca cagccactca tcggctacca cggcatcatc ggcatggagt
     1261 cctatgagcc gggcaaaact tcctcttttt acggagaatt gggctaccat caacgaggct
     1321 ccagcaaata tgtccccgcc tatcagtcca acgccggcat taattacaat tcctatacga
     1381 ttaggaatgt atttcataac gtaggtcttg cacttggcgg aaggaaatcc ttcggtccaa
     1441 acaacaaatt cttttacaac ctggctctac gcggcgaata caccatcaaa gctcagttgt
     1501 cggatgtttt tgtcggattc tcagagtttg tcagaaaatt caattatggc gtagatgtcg
     1561 gagccgggta taatttcccg atcggttatc acatgggctt tgttcagatc caattgcagc
     1621 cagacctgtc caaacaaatc tggtcaacac ggtattccgg ctatgattat cagggcaatc
     1681 ctgttacata tcccgagcaa aaggttataa attatacatt cgagttgtca ctcggattca
     1741 ggcttttcgg aaatctcatc ccggaagatt gattcgtttg ttcctgacaa aaactccttg
     1801 tgcatcatga gtaatttaac ctccgatcaa tttagtttta tagtacggga tgtaacccga
     1861 ttgtctcagt ccgtcaacag tcatctggac aggcttttgt ccaatcatgt tatcctgctg
     1921 gaagcgccga tgggtgcggg taaaaccaca ttcgtctctg cattgacagc tctgctgggc
     1981 agcctgatta caccatccag tcctacattc tcgatttgca atgaatatcc tttggctagt
     2041 cctgtttctg gatatactca aatcgtccat atggacctgt accgtttcaa atcaatagag
     2101 gaagtcctta ccagtggcat cgaggattat ctgtacgatc cccaaagcct ggtcgtcata
     2161 gaatggccgg aactgattac ctctctggtt gtcgagccat atacttatat aacaattgag
     2221 gtgatggatg atctgcaaag gaaattcgta tacagcattg attgacccaa aattttcttt
     2281 cccgaattct cgttttttat tcaattagag gtttttacaa ccgaattttg cttagcttcg
     2341 attcaggaaa caaacatgta atattgcttg gatacaaccg tacttaccta tcgatttgct
     2401 ccggaggctc ttccttttcg ggaagtggtg tgttatgtgc ttaagttgat acaggatcgg
     2461 ctggaggttg tatttgaaga aaatgaatca ggccagctgt gttttggcaa tggagacaat
     2521 gaatatccgc taatgcaatc gatatacgag ccgctttccc gcctaaaacc ctgcgaggag
     2581 aaatacctta gcgggattta tatattttat gaaggtaaaa tggatattct tgccagcatc
     2641 ttttttatcg tacattgcct cggcgaatcc atttccggca atacgccgga tcaatatggc
     2701 agaatcgttt ttcatcaaag cagatatggc agccaacatg gaccgaatga tgatctcgtc
     2761 actccgctga tgcgtgagtt cctccttgga cactttggcc tggacagcat taaggccaaa
     2821 gtgaaaaaac aggttatctt atctcacgat attgatttat tgacttcggg actacgcaga
     2881 gagttgagtt tcttttttcg aaaaccatcc tttaccctgg ctaaagctct gatgcgtcat
     2941 gttctgaccc gggataagat ctggggggat gcttcctcta ttcttggtcc cgagaaagat
     3001 tgtggctaca agtcggtgtt ttatttctta ccggaacacc ggacacacaa tggaatcgtc
     3061 aatgcggatt acgatatgga ggcactgcat cgctacgatg accgtatcct tgctgccggt
     3121 tgtgaagcag gacttcataa aagcagctct gacgagagtt atagcgtgga aaaggagaga
     3181 atgggcctat cggtagattc caacagaaac cactatctga ggtatagatt acccgatgat
     3241 tggaagcgaa tggccgaggc cggctttcgg acggataccg gcctcggctg gtccgacagt
     3301 ccgggcttga gaaatggctt cccttggggg tttaagccct tgggaacaaa ccttaccgtc
     3361 atccctttgg tgttgatgga tacctatttt gaccggaatg acattgcgga acaaatcacc
     3421 gaaaccttcc gttccatgat ttccggctgg ccggatggat ttcaagtatc tgtcttattc
     3481 cacaacaatt atctgacccc ctggtccaac cgggcatttc ttgagcaata catcacactg
     3541 ttagactttc tgaagaagga aggaatagaa gtcgtcacac catcttattt ttcataaatg
     3601 atgaaccaca aattaaaccg attgatatgc atatcctgat gattccggca tggtttcaac
     3661 cggagtcagt cgggcatcac ggtatctttg tgcgggacat tgccagggca cttgcgcatt
     3721 gcgggcatac ggtacgtatt gtatcatttg cctatggcaa taaatatctg gaagttgatg
     3781 atggtaacat ccttgaaata tttcatccga ctgcagggtc gggtttattc agtaaattga
     3841 gctatctaaa gaagacaggt ccttggttgg atgaatattg caggagatat ggcaagcccg
     3901 atatcatcaa tacacacggg ctttcttcca ttaggctggt gagtcgatgg gcacatatcc
     3961 attccctgcc gattgtacac cacgagcatt tgaaggcatt gatcaatccc ggacttccat
     4021 ttaggatcag gtggcaggcc cgctacttct atcagaaggt ggcaacaatc atcgctgtaa
     4081 gcacaattca tgcggatgcc atccgcaaat tgaccgatac ggccgtccaa aggataccgg
     4141 atgtgattga ttctgctttt tttaatattc cgttggccaa aaacctcaaa accggccttc
     4201 agttggcctg cataagcgac cttgatccgg aaaagggata cgaattgttg atagaagccg
     4261 tgggtctgct tgcagcgaat aacatcgact ttaccttaca tattgtcgga gacggccccc
     4321 tgcgttccat gatggagcgg tggtctgaag aaaagggaat tcgacacagg ataaaccttc
     4381 atggcagaca aaatcgggca ggagtgttgt ccatacttga aaaatgccac atctactgta
     4441 ctgctaccat taccgaaagt ttcggtatgc atattgcaga ggcattggct gccggccttt
     4501 atgttgtcac tacagattgc ggcagtgtgc atgattatat ctcaaaaacc aacggaacca
     4561 ttgtgcctca gcgttcggct gtattgctgg cagatgccct gcaaacagcg atcaccaact
     4621 attctgttga aaatgttcat gccatccgtg cctccatagc agaatttgcc tcggcagaag
     4681 tagtcattcc tcagatagaa tcggttttca gaagcgtatt gagacaaagg cgataaaatg
     4741 cagctatgat atctgaattt ggcggacctt gctttgagtg tcatcttcca ctgcagaaaa
     4801 tggatacctc ctgactttgt caatgcgtca ccctggcaaa aacgctctaa gtcttaagag
     4861 aatacctgat ataaaatctt cccggtaaaa cttagaattt tgcatgattc aaatattttg
     4921 tttaagttta
//
LOCUS       DAOQMC010000171         4916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_7998_length_4916_cov_4.602345, whole genome shotgun sequence.
ACCESSION   DAOQMC010000171 DAOQMC010000000
VERSION     DAOQMC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7998_length_4916_cov_4.602345"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKV31_07420"
     CDS             <1..463
                     /locus_tag="PKV31_07420"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNB92724.1"
                     /translation="IQSGNKFNLSMKDMAIISDGKTVWFINKKQKEVQINDYSKDVSQ
                     GMVNPAEFIMQYSPKSFISAVTFDGPKNGKKVMVMEIKSVDRKVDYSKVRVTVNKATN
                     ELMEMELFNKDGSRYTLNVSKTTFSKTTSSSSFGFDKSKYPGFNVEDLRVN"
     gene            486..1988
                     /locus_tag="PKV31_07425"
     CDS             486..1988
                     /locus_tag="PKV31_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNB92725.1"
                     /translation="MKIRSKIIKPIAKVIANRIDRWKRTAVADQEHIRRRLVERGRNT
                     VYGKEHQFDKIGSSSAFSTYVPLIDYEGLKPYIDRIKQGERNILWPGRPKYFAKTSGT
                     TSGVKYIPLTRDSMPNHFGSARNALLNYAAKTGKAGFFDGKMIFLSGSPELDTSSVIP
                     TGRLSGIVNHEVPAWLRKSQVPSYNTNCIEDWELKVDAIVKETVNLDMRLISGIPPWV
                     QMYLERLLEVSGKKDIMHLFPNFSMFVYGGVNYEPYRNTLEKLFGTSVDSVETFPASE
                     GFFAFQDEQDDSSLLLNTQSGIYFEFVELEEMARDKPTRVPLEGVRIGVPYAIIVNNN
                     AGLWGYNIGDVVTFTSVDPYKIVFTGRVSHYISAFGEHVIGKEVEDSLKEAIGDTGVK
                     IAEFSVAPQVNPGGGRLPFHEWLIEFEVMPADLEAFRLKIDSALARRNIYYNDLITGN
                     ILQTLQIVPLKKGSFITYMRENGKLGGQNKVPRLTNDRKIADALHEINNR"
     gene            1991..2494
                     /locus_tag="PKV31_07430"
     CDS             1991..2494
                     /locus_tag="PKV31_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92726.1"
                     /translation="MKNNISALSLLIMLCVGTMHAQTVEFIGKKIQVPKDCEKVSQYE
                     IDCGGFSLSWAYMSKGTGDNVIFDQIGRLKQRPGMQYKPMRILVDSVETNGFVASFSA
                     DNVKYFMLMTSGEVRGKHFLLQAIDVQPFWRYEGYNAVFDSLVKVLPENEGPGKGKEP
                     EGVKNKE"
     gene            2491..2817
                     /gene="cutA"
                     /locus_tag="PKV31_07435"
     CDS             2491..2817
                     /gene="cutA"
                     /locus_tag="PKV31_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNB92727.1"
                     /translation="MMAEQKLISVISIYCGTESEAVSLSQALVDKHLAACCNISTCAS
                     LYKWQGEMNEDLEWIVSVKTMARAIDKVMELIKSEHTYDLPSILIDTLATTEEYYNWV
                     NESVLF"
     gene            complement(3066..3266)
                     /locus_tag="PKV31_07440"
     CDS             complement(3066..3266)
                     /locus_tag="PKV31_07440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNB92728.1"
                     /translation="MSKKATKTLTELLDELRQIQISVESGNIDIDQIPYLIQRATAIK
                     EECEARLTDIDSIINAAGNKEV"
     gene            complement(3259..4509)
                     /gene="xseA"
                     /locus_tag="PKV31_07445"
     CDS             complement(3259..4509)
                     /gene="xseA"
                     /locus_tag="PKV31_07445"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNB92729.1"
                     /translation="MTTDIKSYTLLELNTYIKRVLALNFDNELWVRAEILNAAEKRGH
                     YYLTLIQKSSVSNDIVAQLEAVVWASDFQRIKKENPDLVNFFTSGFEIMSHGQPVFHE
                     RFGLQFRISDINTEYARGALDRRKEELFNRIRTEGLDKINKTLPLPPAILRLAVISSE
                     NAAGYIDFVQHIEQNKFGLKFRITLLDSVMQGTSLEETISKSLNFIEKRKSEFDAVAI
                     IRGGGSKVDLAAFDNYRLAMLIAHFPLPVITGIGHEIDVSAMDLAAGTSLKTPTAVAE
                     FLINHNIAFLAEIDDRYRKILSGFSVSILVYKNAVEQATMFIRSTSSLLTDRLRHTVQ
                     LTMGKITSGSTAIVRDFRHTMQIAYRSLQLSDPQNILKKGYTLVFQNKDKISLKSDFK
                     DDSNTTIYWADGKVTGKFKIEENE"
     gene            complement(4506..>4916)
                     /locus_tag="PKV31_07450"
     CDS             complement(4506..>4916)
                     /locus_tag="PKV31_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92730.1"
                     /translation="KIFYGEGTSFFLISDQKDNAIAMISEYTLFVNSEKKISDDIHTY
                     LTNIPWDSNSAIYAGYNNEFEKHHFYKDISSFATGRKIPEITFKHQTGDFDTASGHAM
                     AMAVDDITKGKYNRIVIFNHYKKKSLHSIIISKP"
BASE COUNT         1422 a          904 c         1087 g         1503 t
ORIGIN      
        1 gatccaatca ggtaataagt ttaacctgag tatgaaggat atggctatca tcagtgatgg
       61 taagacggtt tggtttatta ataaaaaaca aaaggaagtc cagatcaatg attattcaaa
      121 ggatgtcagc caaggtatgg tcaatccggc tgagtttatc atgcaataca gtccaaagtc
      181 ttttatttcg gctgtgactt ttgacggtcc aaaaaacggc aaaaaggtca tggtgatgga
      241 gattaagtca gttgaccgga aggttgatta ttccaaggtc cgtgtcacag tcaataaggc
      301 aaccaatgaa ttgatggaaa tggaattatt caataaggat ggtagccgat ataccttgaa
      361 tgtttcaaaa acaacctttt ccaagacaac ttcgtcctca tcctttggat ttgataaatc
      421 caagtatccg ggcttcaatg tggaggactt acgggttaat tgaaacgcaa taaatggtgg
      481 caggaatgaa gatccgttct aaaattatca aacccattgc aaaagtaatc gctaatcgga
      541 ttgaccgatg gaagcgaact gctgttgctg atcaggaaca tattcgacgt cggttagtcg
      601 agagaggcag aaatacagtt tatgggaagg aacatcagtt tgataagatt ggtagttcga
      661 gtgcgttctc aacctatgtt cccttgattg attatgaagg tctcaaaccc tatattgata
      721 gaatcaagca aggtgaaaga aatatacttt ggccgggacg acccaagtat tttgcaaaaa
      781 catcgggcac cacatccggt gttaagtata taccgcttac aagggatagt atgcctaatc
      841 atttcggctc tgcgaggaac gccctgctta attatgctgc caaaacagga aaggcaggtt
      901 tttttgacgg taaaatgatt tttttaagtg gatcacctga gttggatacg tcatctgtga
      961 ttccaaccgg tcgcctttca ggtattgtca atcatgaagt accggcatgg ttacgtaaaa
     1021 gtcaggttcc ttcctacaat acaaattgca ttgaggactg ggaactaaaa gtcgatgcga
     1081 ttgtcaaaga aacagtcaac cttgatatgc gccttatttc aggcattcct ccctgggtgc
     1141 aaatgtattt ggagcgtctc cttgaagtca gcggaaagaa agacatcatg catctgtttc
     1201 caaatttcag catgtttgta tatggcggtg tgaattatga gccatacaga aatacattgg
     1261 agaaactatt tggtacatca gtagattctg tcgagacttt tcctgcctct gaaggcttct
     1321 ttgcctttca ggatgagcag gacgactctt ctttgttgtt gaatacccaa agcggcatct
     1381 atttcgaatt tgttgaatta gaggaaatgg ccagggataa accgacacgt gtgcctttgg
     1441 aaggtgttag gattggtgtg ccttatgcaa tcattgttaa caacaatgcc ggtttgtggg
     1501 gttacaacat cggcgatgta gtcaccttta catcggtcga tccgtataag attgtattta
     1561 ccggccgtgt atcacattat atttctgctt tcggggagca tgtgattggc aaggaagtag
     1621 aagatagtct caaagaagca ataggagata ccggcgtgaa gatagccgaa ttttctgtcg
     1681 cccctcaggt caatccgggt ggtgggcgac ttccttttca tgagtggctt atagaatttg
     1741 aagtgatgcc tgccgacctt gaagctttta gattgaagat tgattcagcg ttagcgcgaa
     1801 gaaatatata ctataacgac ctgatcaccg gaaatatcct gcagacactg cagatagttc
     1861 cgcttaaaaa agggtcgttt atcacttata tgcgtgagaa tggaaaactg ggtggccaaa
     1921 acaaagtgcc acgcctgaca aatgacagaa aaatagccga tgctttgcat gagataaaca
     1981 atagatgatc atgaaaaata atatatccgc cctttctttg ctgattatgc tttgcgtagg
     2041 aacaatgcat gcgcagacag tggaatttat tggaaaaaaa atacaagtac ccaaagattg
     2101 tgaaaaggta tcgcaatatg aaatagattg tggtggtttt tcactttcct gggcttatat
     2161 gtccaagggt accggagata atgttatttt cgaccagatc ggccgattga aacagaggcc
     2221 aggcatgcag tacaaaccga tgcgaatcct tgtggacagt gttgagacca atggatttgt
     2281 tgcttcgttt agtgcggata atgtcaaata ttttatgttg atgacttccg gtgaagtgag
     2341 aggtaagcat ttccttttac aagcgattga tgtgcagcca ttctggagat acgaaggcta
     2401 taatgctgtg tttgacagtt tagttaaggt cttgccggaa aatgaaggcc ccggtaaagg
     2461 aaaagaaccg gaaggtgtta aaaataagga atgatggcag aacaaaagtt gatttcagtc
     2521 attagtattt actgcgggac ggagtcggaa gcagtatctc tgagtcaagc cctggtagac
     2581 aagcatcttg cggcatgttg caacatttca acctgtgctt cgttgtataa atggcagggt
     2641 gagatgaatg aagatctgga atggatagta agtgtcaaaa caatggctcg tgctatcgac
     2701 aaggtcatgg aactgatcaa atcggaacat acctatgacc tgccttccat cctcattgat
     2761 acattggcga ccacagaaga atattataat tgggtgaatg aatctgtttt attttaaaag
     2821 gtttacagga tgatgaagtt tttaaagaaa ggatatctca agaatgcagg aatagggttg
     2881 tttttgtttt tttttctgaa aggattggca tggattgccg tattttattt tggcattaac
     2941 atcttttccc gttgtagtta aaattttatt tcaaggtgag aataaattaa atgcatcacg
     3001 aggattccag gaacgaagag tgctcccttt atttttgcca ttgattaatc aacatcaata
     3061 ttttttcaaa cctctttatt tcctgcagca ttgattatgc tatcgatgtc cgtgagtctg
     3121 gcttcacatt cttccttgat tgcagtcgca cgctgaatta agtaaggaat ttgatcaatg
     3181 tcgatattcc cggattcaac ggaaatctgg atctgcctta actcatcgag taactccgtc
     3241 aaggttttag tcgctttctt actcattttc ttcaattttg aattttccgg taaccttgcc
     3301 gtcagcccag tatatggttg tgtttgaatc atctttgaaa tcacttttca atgagatttt
     3361 atccttattc tggaaaacca gcgtgtaccc tttttttaaa atattctgtg ggtctgaaag
     3421 ttgaagactt cgataagcaa tctgcatggt atgacgaaaa tcacgaacta ttgctgtaga
     3481 accggaagtt attttaccca tggtcaattg aaccgtatga cgaagtctgt ctgtcaacag
     3541 ggaagaagtt gaacgaataa acatcgttgc ttgttctaca gcatttttat ataccaggat
     3601 actgacggaa aaacctgata gaatttttct gtagcggtca tcaatttcag ccagaaatgc
     3661 tatattgtga ttgatcagga attccgcaac tgctgtaggg gtctttagag aggtgccggc
     3721 tgcaagatcc atggcgctga catcaatttc atgcccgatg cctgtaatga caggtaaggg
     3781 aaagtgcgct atcaacattg ctaaccggta attgtcaaat gcagccagat ccaccttgct
     3841 gccaccgcct ctgataatgg ctacagcatc gaattcggat tttctttttt caatgaaatt
     3901 caacgatttg ctgattgttt cttccagtga ggtcccttgc attacgctat ccagtaatgt
     3961 aatccggaac ttcagaccaa atttattttg ttcaatatgt tggacaaaat cgatatatcc
     4021 ggctgcattt tctgatgata ttacagccaa acggaggata gcgggaggta agggtaaagt
     4081 tttgttgatt ttgtcaagcc cctctgtccg aatcctattg aataattctt ccttccgccg
     4141 atccaatgcc cctcttgcat actctgtatt gatatcagaa atcctgaatt gcaggccgaa
     4201 tctttcgtga aataccggtt gcccatggct catgatttca aatcctgacg taaagaaatt
     4261 gaccagatca ggattctctt ttttgattct ttggaaatcc gatgcccata ccactgcctc
     4321 taattgagct acgatatcat tggagacgga acttttctgg atcaaagtca ggtagtagtg
     4381 acctcttttt tcagccgcat ttaatatttc agctcttacc cacagctcat tgtcgaaatt
     4441 caaagcaagg accctcttga tgtaggtatt caactccaat aatgtatagg atttgatatc
     4501 agtagtcatg gctttgatat tataatcgaa tgcagtgatt tctttttata atgattaaag
     4561 ataacgattc tgttgtattt ccccttagtt atgtcatcga ctgccattgc cattgcgtgg
     4621 ccggaagccg tatcaaaatc tccggtttgg tgcttaaatg taatttccgg tatctttctt
     4681 ccggtagcaa aagatgaaat atctttatag aaatgatgtt tttcgaactc attgttataa
     4741 ccggcataaa ttgctgaatt gctatcccat ggaatgttgg tcaggtaagt gtgtatatca
     4801 tccgatattt tcttttccga attaacaaat agagtatatt cggagatcat tgcaatggca
     4861 ttgtcttttt ggtcggagat aaggaaaaaa ctggtgcctt cgccataaaa gatttt
//
LOCUS       DAOQMC010000172         4863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8147_length_4863_cov_4.471298, whole genome shotgun sequence.
ACCESSION   DAOQMC010000172 DAOQMC010000000
VERSION     DAOQMC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8147_length_4863_cov_4.471298"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1407
                     /locus_tag="PKV31_07455"
     CDS             202..1407
                     /locus_tag="PKV31_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNB92731.1"
                     /translation="MNCFRLILLCLLPVFAFSQPCSTGCDGKRYIEYVFPEYEITSMK
                     YARPIPENFDLYLDVYQPKGDQLEKRPMILFVHGGGFVSGSKADMAGFCIDYVKRGFV
                     TASIDYRLMFGIPDQAGFIRGVVKAMNDFKAAYRFFKVDASGANQFRIDTTKMMIGGY
                     SAGAVTVLHSAYLDSGDYVPPIILNEINAQGGWKGNTGDTENLSHREDDILGVFNYAG
                     ATFTKELFDEGDPFLMSYHGDIDNTVPIDSADFFGFATAYGSRVLHPKCAEKGIPQTL
                     EVVPGGGHTEIFTDPNLWPYLLEFVGKAKDIYYPRICGTPANTGTLADEIAATAFPNP
                     SSSKIKITFDRNVDGVLIYDRYGRLMSKHTPSGHVIEVEKHELIPGIYFAIPVIDGRQ
                     YRAVKIEFY"
     gene            complement(1401..3260)
                     /locus_tag="PKV31_07460"
     CDS             complement(1401..3260)
                     /locus_tag="PKV31_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HNB92732.1"
                     /translation="MSIFRFRLSAIKQWPDIFKNLFSNYFLVVTTALILAGLWIYQIE
                     AGDKLSFFASHNVSRILFSLGLCIPAFLALHVLVNKQNQKILYFTLGLGTIILMLGIY
                     YVNYELRADKQFYRTLALFLAFHGLLSLSAYRKYSSVRDFWQFNTNLLLRTIGSLFFT
                     LVLYLGVIAAIAALNNLFGVHISYNLYFDLMIVALVVYNTFFVLAGMKTSFDESGSEF
                     QYPLSLKVFTQFVLIPLMALYAIILYAFSIKIIVLWSLPKGWIAYLVLLFSIAGILGF
                     LLIYPIRNSTEKKWVRIYSKTFFIALVPLILLLHVSIWTRIGQYGFTIHRYVVAILAI
                     WLTLIPGYFLLSKKDNIIVIPASLTALFLLACFGPWGFDAISQKSQQNRIEKIMHDAQ
                     IQDYYKKGKINEYKANDKVTTELNSIIDYLLLNHGARSMKGFDIASWDKIADSIIDRQ
                     QKFFEEPKMEKGDTAISNSINASSFYSSPNSYTFLKEINVPQSGGYNMARFDYSYSPL
                     QNQIEIISYSRLVPFDLNISFKDQNNIITLDTSGFKLNINGKRITADAEKTVIDALNK
                     CGTACFNAQSPIIVTGKQMDIEYRIIINSLSGKIISDQKYEFENAMGYLLFNQ"
     gene            3583..4833
                     /locus_tag="PKV31_07465"
     CDS             3583..4833
                     /locus_tag="PKV31_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB92733.1"
                     /translation="MAIVQNVRHLFVNAYSGIKREVWLLSLIMLINRSGALVFPFLSI
                     YLTRHLGFSEIQAGWVLSAYGVGSMVGTLTGGWLTDKYGSFRTQFGALVLSGIGWIIL
                     AHITEYHHLLMLAFIQSSIGDIFRPANNSSITLFTDAGNRTRSFSLNRMAMNLGYSIG
                     PAIGGIFATISYTLLFWGDGLSCIFSSIAFFIIFRRYFTKKGPVEEDRPEIPVRGETT
                     GPLKDTIFLVFVISVILFATVFFQMLFTLPIYYAQHYKIGEAHIGTLLAVNGLFVFVS
                     EMAFVHYVGNRIENHKLIIAGVLATGLSFIMLNWVHHSAWLIVAMIVLSLAEILSMPF
                     MQTYVANRATQSNIGRYLALYSFAFSVAFIFAPPLGMWTIKNFGFHMLWNGCMAVAAI
                     NTVVIYFVFKADKHRKASLKSERG"
BASE COUNT         1356 a          914 c         1037 g         1556 t
ORIGIN      
        1 aaggttgtta gtatccggct gtaggctgat tttgattaaa taaggttgac attaataata
       61 atagcccatg tatgttatgc tgtcatttga tgtcgggggt ttattggata atcattttca
      121 agtctaagta aattaatgtt aacgaagcgc ctttatagtt gaataatatt tacatttacg
      181 cctgataatt gattaatatc tatgaattgt ttccgattaa tattactgtg tcttcttccg
      241 gtttttgcct tcagccaacc ctgttccaca ggatgtgatg gcaagcggta tattgaatat
      301 gtctttcctg agtacgaaat cacttccatg aaatatgcga ggccgatacc ggagaacttc
      361 gacctatacc tcgatgtata tcaaccaaaa ggagatcagc tggagaagag gcctatgatc
      421 ctttttgtcc atggaggagg ctttgtgtca gggagtaagg cggatatggc cggattttgt
      481 attgattatg taaaacgcgg tttcgttaca gcctccattg attatcgatt gatgtttgga
      541 attcccgatc aggccgggtt tatacggggc gttgtcaaag caatgaatga cttcaaggct
      601 gcctataggt ttttcaaggt tgacgcttca ggcgccaatc agtttagaat tgataccacg
      661 aaaatgatga ttggcggata ttctgccggt gcggttactg ttctgcattc ggcctatctt
      721 gactccggag actatgtgcc gccaataatc ctcaacgaga tcaatgccca gggtggttgg
      781 aagggcaata ccggagatac agagaatctg tctcatcggg aggatgacat actgggtgta
      841 ttcaattatg cgggagctac ttttaccaaa gagctctttg atgagggtga cccttttctg
      901 atgagctatc atggtgatat tgataatact gttccaatag attcggctga tttctttggc
      961 tttgcgactg cttatgggtc aagagtcctg catcctaagt gtgctgaaaa gggtatcccc
     1021 cagactctgg aggtagtgcc aggcggtggc cataccgaga tatttaccga tccgaatcta
     1081 tggccatacc tgctcgaatt tgttggcaaa gctaaagata tctattatcc ccgcatctgt
     1141 ggtacgcctg caaatacagg gaccttggca gatgaaattg cagctactgc ctttcccaat
     1201 ccttcctcct ccaaaatcaa aataacattt gaccggaatg tggatggagt attgatctat
     1261 gacagatacg gaagattgat gagcaaacat actccatccg ggcatgttat agaggtggaa
     1321 aagcatgaac ttataccggg catttatttt gcaatacctg tgattgatgg caggcaatac
     1381 agggcagtca agattgagtt ctattgatta aaaagcagat atcccattgc attctcaaac
     1441 tcatactttt gatctgaaat tattttgccg ctcaggctgt taataattat tcgatattca
     1501 atatccattt gctttccggt tactataatg ggtgattggg cattgaaaca tgctgttccg
     1561 catttgttca aggcatcgat gactgttttt tcagcatccg cagtaattct tttgccgttg
     1621 atattaagtt taaaccctga agtgtccagt gtaatgatat tattttgatc cttgaatgaa
     1681 atattcagat caaatggtac caatctggaa tatgaaatga tttctatttg attttgtagg
     1741 ggggaataac tataatcaaa cctggccata ttatatccac ccgattgagg tacattgatt
     1801 tccttgagaa atgtatagct attgggagaa ctgtaaaatg aggatgcatt gatcgaattg
     1861 ctgatagcag tatcgccttt ttccatcttc ggttcttcaa agaacttttg ttgtcggtcg
     1921 atgattgaat cggctatttt atcccaactt gcgatatcaa aacctttcat tgatcttgct
     1981 ccatgattta gtaaaaggta atcaataatg gagtttaact cggttgtaac cttgtcattt
     2041 gctttgtatt cattgatttt acctttttta taataatcct gaatttgggc gtcatgcata
     2101 attttttcaa ttctattttg ctggcttttt tgactgattg catcaaaacc ccatgggcca
     2161 aaacaggcta aaaggaaaag tgctgtcagg gatgccggaa taactattat attgtctttt
     2221 ttagaaagga ggaaatatcc tggtatcaat gtcagccata tggccaggat ggcaaccacg
     2281 tatctgtgga tggtaaatcc gtactggcca attcttgtcc agatactgac atgtaacaaa
     2341 agtatcaagg ggacaagagc tataaaaaat gttttggaat aaatccttac ccattttttt
     2401 tccgttgagt tcctgattgg atatatcaac aggaagccaa ggattccggc gatggaaaat
     2461 aaaaggacaa ggtaggctat ccaacccttt ggcaatgacc ataatacaat aatctttatc
     2521 gaaaatgcat acagaatgat tgcatataat gccatcaaag ggattaaaac gaattgtgtg
     2581 aagactttta gagaaagcgg gtattgaaat tccgaaccgg attcatcaaa cgaagtcttc
     2641 attcctgcca acacaaaaaa tgtattgtag acaaccaatg ctacaatcat cagatcaaaa
     2701 tacaaattat aggagatatg gaccccgaat aggttgttca atgctgctat tgccgcaatt
     2761 actcccagat acagcaccag ggtaaagaaa agtgaaccaa tagttcttag caacaaattg
     2821 gtgttgaatt gccaaaaatc cctgaccgag ctatattttc tataggcact aagcgatagc
     2881 agaccatgaa aagccagaaa taaggccaat gttctgtaga attgcttatc agccctcagc
     2941 tcgtaattca catagtagat tccaagcatc aaaattattg ttcccagtcc taacgtgaag
     3001 tataagattt tttgattttg cttatttaca aggacatgaa gagctaaaaa tgcgggtatg
     3061 cataatccaa gactgaataa tattctggaa acgttatggg atgcaaaaaa agacagtttg
     3121 tctccggctt caatttgata aatccatagc cctgccagga tcagggcagt tgtcactaca
     3181 agaaaataat tgctgaatag attcttgaag atatcgggcc attgcttaat tgctgaaagt
     3241 ctaaatcgga aaattgacat tgaattttaa tcttaattgt tgtttaaatt tttataaaaa
     3301 tactcttatt tcacatatta cgcactacat tcctgcattt ggatttgtta gtaataactt
     3361 gagcatgttt aaataaaggg aatttaatga tcatgaaata atacactgta ttgacttaga
     3421 ctttgggtgc aatgttcatt aaagtggttg ccgtccggga agatatgttc acctccctgt
     3481 tgagccttgc gctatgctat aagctgatca gataagtatt atctgaaata ttgtcaggac
     3541 taactatttt tgttccctta ttctcatttt taaatcaatc tgatggccat cgtccaaaac
     3601 gtgagacacc tctttgtcaa tgcctattcc ggcattaaaa gagaggtttg gttgctgtca
     3661 ctcatcatgc tgatcaacag atcaggtgcg ttggtctttc cattcctgag catctacctt
     3721 accagacatc ttgggttttc tgagattcag gcgggctggg tgttgtcggc ttatggcgtc
     3781 ggctcaatgg ttggtacttt gacgggtggc tggctgacgg ataaatatgg tagttttagg
     3841 acacaattcg gtgcacttgt tctaagtggc attggatgga taattctggc acatattacc
     3901 gaataccatc acctgctgat gcttgccttt atccaaagtt ccattggcga tatcttccgg
     3961 cctgccaaca acagttcaat caccctattt acggacgccg ggaatagaac caggtcattt
     4021 tcactaaaca ggatggcgat gaatctggga tattccatag gtcctgccat tggaggtatt
     4081 tttgccacta tttcctatac cttgcttttt tggggagatg gtttgtcctg tattttttcc
     4141 tctatcgctt tctttattat ttttagaagg tattttacta agaaggggcc cgttgaggaa
     4201 gatcgaccgg aaattcctgt gcgtggtgaa actaccggtc ctttgaaaga tacaattttc
     4261 cttgtttttg ttataagtgt cattcttttt gccacggtat tctttcaaat gctctttacc
     4321 ttaccgattt actatgcaca acactacaag attggtgaag cccatatcgg aacactgctt
     4381 gctgtgaacg gactttttgt gtttgtttca gagatggctt ttgtacatta tgtagggaac
     4441 cggattgaga atcataagct catcattgcc ggtgttctgg ctaccggcct gtccttcatt
     4501 atgctcaatt gggtacatca ctccgcatgg ttgatcgtgg caatgattgt gctcagtctg
     4561 gcagaaatac tttccatgcc gtttatgcag acctatgttg ccaatcgggc tacgcaaagt
     4621 aatattggca gatatctggc tctgtattct tttgcgttca gtgttgcctt tattttcgct
     4681 cctccactcg gtatgtggac gatcaagaat tttggatttc atatgttatg gaatggttgc
     4741 atggcagttg cggctatcaa taccgtcgtt atttactttg tattcaaggc agataagcac
     4801 aggaaggcct ccttgaaatc tgaaagggga taaatattat aacttccggg gaaaatcagg
     4861 tag
//
LOCUS       DAOQMC010000173         4822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8268_length_4822_cov_4.574785, whole genome shotgun sequence.
ACCESSION   DAOQMC010000173 DAOQMC010000000
VERSION     DAOQMC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8268_length_4822_cov_4.574785"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKV31_07470"
     CDS             complement(<1..520)
                     /locus_tag="PKV31_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB92734.1"
                     /translation="MKRRTDIFLMTVLMCCLSVFGYSQSRTVTGVVTDELGQPLDGAS
                     YVVKSTSASGITGADGSFSVMVSGNNDVIEFTYVGFKSAEITVGEGSTLTVVMQRSDG
                     QLETVVVTALGIKRESKSLGYTVQEVKGEAIAASREVNVANTLSGKVAGLQVIKSSNG
                     PAGSSKIVLRGFN"
     gene            complement(548..1780)
                     /locus_tag="PKV31_07475"
     CDS             complement(548..1780)
                     /locus_tag="PKV31_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNB92735.1"
                     /translation="MAEITNLKNKIARIKILRQLFFTPGLTSSEISNAIDKSLPVTTQ
                     LLTELIQEGRIEETGLAGSTGGRRPQTYSLADGSFQILSVAMDQLVTRIALVDEKARV
                     VGTRQLFELNLYKNPDSLSELIECIRLFINSENVDTSRILGIGIGMPGFVDVKKGINN
                     SFLPSAEGSVIEIIEKAIGLPVWIDNDSSLIALAESKWGAAKDKKHVMVLNIGWGIGL
                     GMLLNGKLYRGQEGFAGEFSHMSLFENDKLCNCGKYGCLETEASLLVTLEKVNKGIVN
                     GAVTSIAMPDLNHKEAAAKSLIKAVLEGDRFAIEVLSESAYQIGRGVAILIHLLNPEA
                     IVISGFGSLAGKAWIAPIQQAINELCIPALAQNVEIRISDLASKAELLGAAALVAENY
                     GKKEQIPDNKQVNLNHSW"
     gene            2224..4509
                     /locus_tag="PKV31_07480"
     CDS             2224..4509
                     /locus_tag="PKV31_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNB92736.1"
                     /translation="MFFFLIYLIPGNTQEIISIIPKPVKLERKAGSFILDESTGYRQA
                     ADSGINQAVRLLTSECTRRSGINLKNTTKASKFISLAIVPGIIPEREGYTLSITPSAI
                     TLKAGDESGLIHGVQTILQLLPAYRTNEVIQLPCMEIYDFPRFPYRSMHLDVSRHFYP
                     PELIKDYIDLISYYKMNTFHWHLVDDQGWRIEIKKYPKLTEIGAWRVDRSNMPWDSRQ
                     PQQPGEKATYGGFYTQNQIREIVKYASSRGVTIIPEIEMPAHVESAVAAYPGLSCVKK
                     PQTVLTGGIYPPDFQTSYCAGDEKVFTFLQDVLMEVIELFPSSYIHIGGDELDKKFWQ
                     ECTACKKRMKDEGLKNVDELQSYFIRRIEQFLQTKGRRIIGWDEILEGGLAPDATVMS
                     WRGESGGIAAAEMGHDAIMTPGTPLYLNHYQAGPEGEPLANGGFNTLKMVYDYDPVPA
                     SLKSEAARHILGAQGNIWGEFIQTRSHVEYMVLPRMAALAEVVWSPASQKDFTDFSKR
                     LISHFNYYEGKGYNYCKGNFTVAIRPISDKGRLSVELTSEVAGCSIHYTTDGSMPGPE
                     SPEYKSPIPITGSCLLHAVTVLDGKVMNTIPATQAFEFHDAIGANVNYEFPYSNSYKA
                     EGPNILTDGIRGGSNIHKYWHGFLGKDLVATVTLPNSIHVNKLSLSCMQHYKDWIFLP
                     TQVDYEISEDGIHFVGVGNVKNDIPVTETQQTIKEFALNLKPVMARYVRVKAHILPGA
                     PEGHPGKGNPVWIFADELKVN"
     gene            4520..>4822
                     /locus_tag="PKV31_07485"
     CDS             4520..>4822
                     /locus_tag="PKV31_07485"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNB92737.1"
                     /translation="MISSLEKIPVSIFKSPLEGSIYAAQQIANLIRARQMEGRNCVLG
                     LATGSTPISMYKELVRMHREEGLSFRNVITFNLDEYYPLERDAIQSYWSFMHNNLFN"
BASE COUNT         1397 a         1103 c         1004 g         1318 t
ORIGIN      
        1 aattgaaacc acgcagaacg attttggatg aaccggcagg accattgctg gacttaatca
       61 cctgcaagcc tgcaacctta ccgctcaatg tgtttgctac gttaacctca cgagatgctg
      121 caatagcttc gccttttact tcctgaacgg tatagccgag agatttgctc tctcgcttga
      181 taccgagtgc tgttacaaca accgtctcga gctgtccatc ggatctctgc atgacgactg
      241 ttagtgtgga gccttctcct accgtaattt cggctgactt aaagcccaca taagtaaatt
      301 caatcacatc gttgttacca cttaccatca cgctgaagct tccatccgct ccggtgatgc
      361 cactggcact cgtacttttg accacatagg atgcgccatc cagtggttgc cccaattcat
      421 cggtcacaac acccgtaaca gtccgggatt ggctatagcc gaagaccgac aggcagcaca
      481 tgagtactgt cattaggaat atgtctgttc ttctcttcat aaaataagtt ttttgatgaa
      541 aataaaatta ccacgagtga ttgaggttga cttgcttatt atctggtatc tgctccttct
      601 tgccatagtt ttcggctaca agggcagctg cacccagcag ttcagccttt gatgcaagat
      661 cagatatcct gatttcaaca ttctgagcca atgccggaat gcacaattca ttgattgcct
      721 gctgtatggg agcaatccat gccttccctg ccaaagaacc aaagccactg ataacaatcg
      781 cttccgggtt gagcagatgt atcaggatag ccacacctct gcctatctga taggctgact
      841 ccgataaaac ttcaatggca aaacggtctc cttcgagcac agctttgatc aatgactttg
      901 cagcagcttc cttatggttg aggtccggca tcgcaataga tgtcaccgct ccattgacaa
      961 tacctttgtt taccttttcg agcgtcacca gcagagacgc ctcagtctcc agacatccgt
     1021 actttccaca gttgcacaac ttatcatttt caaacaatga catatggctg aactctccgg
     1081 cgaatccttc ctgacctctg tacagcttac cgttaagtag cattccaaga ccgatacccc
     1141 agccgatgtt gagtaccatg acatgtttct tatcttttgc agcaccccat ttggactcag
     1201 caagtgcaat caggctggaa tcgttgtcaa tccacaccgg aagacctatt gccttctcta
     1261 tgatctcaat gacagatcct tcagccgatg gcagaaatga attgttgatc cccttcttga
     1321 catctacaaa accgggcatt ccaattccaa taccaagaat cctgcttgta tcaacattct
     1381 cagaattgat gaaaagtcgt atacattcaa tcaactcact caatgaatcc ggatttttat
     1441 atagattcaa ttcaaaaagt tgcctcgtcc ctacaacacg ggctttttcg tccaccaacg
     1501 caatcctggt gactaattga tccatggcca cactgagaat ctgaaaactc ccgtctgcca
     1561 gcgaatatgt ctgtggcctt ctaccaccgg tcgaaccggc caaaccggtc tcctctattc
     1621 ttccttcctg gattaattcg gtaagcagtt gagtggtaac gggcagactt ttgtcaatgg
     1681 cattgctgat ctctgaacta gtcaaacccg gagtaaaaaa taattgacgg aggattttaa
     1741 tccgcgcaat tttgtttttc aggtttgtaa tttcagccat tttatttacc aaaagattaa
     1801 caaaaatata aaatagttac attacttttg taaaaattta taaaacttat taaatatttt
     1861 tgataacttt gtgtaataaa atgattgtca gatatttgcg tggtaataaa atatttaatt
     1921 atttttattt ttttcttttg taaaaagaat tggatacgca aagatgtttt aaaaacagtc
     1981 catttatcaa ctttttataa cgatgatgga tttttttaca aatatttgtt tgttagataa
     2041 aattcggaat tggcctgaat tttcccagat aaaatgggca aatagaaccg gaatcatgaa
     2101 agcaatccga taaatgaccg aaaatattta aggaaaaaac cagaaaatga tattaaattt
     2161 ataaaaagga aaaagaccat tcagccctgc ctcacctttt cttgtcatac aatttttccg
     2221 gtattgtttt tttttctgat ctatttgatc cccggcaaca ctcaggaaat tatttccatc
     2281 atcccgaaac ctgtgaaact ggaacgcaag gcgggaagtt ttatccttga tgaatcgaca
     2341 ggatacaggc aagctgcaga ttcaggaatc aatcaggctg tcaggttact tacctcagag
     2401 tgcacacgcc ggtccggcat caatttaaaa aatactacaa aagcttcaaa attcatttcg
     2461 ctggctattg ttcccggtat cattccggaa cgggagggat ataccttatc tataacgccg
     2521 tcagcgatta ctttgaaagc aggtgacgaa tcggggttga tccacggtgt ccaaaccatc
     2581 cttcagttat tgccggcata caggaccaat gaagtaatac aattgccttg tatggaaatc
     2641 tacgattttc cacgtttccc ttatcgcagt atgcatctcg atgtaagccg gcacttttat
     2701 cctcctgaat taattaagga ttatatcgac ctcatctcgt attataagat gaataccttc
     2761 cactggcatc ttgtcgatga tcagggatgg cgaatcgaaa tcaagaaata cccaaagctg
     2821 actgaaatag gagcatggag agtcgatcga agcaatatgc cctgggatag ccgacaacct
     2881 caacaaccgg gcgaaaaggc gacttatgga ggtttttata ctcaaaatca gatccgtgaa
     2941 attgtcaaat atgcttcttc aaggggcgtt accattatcc ctgaaataga aatgcctgct
     3001 catgtggaat cagcagttgc agcctacccc gggctgtcat gtgtcaaaaa accacaaacc
     3061 gtccttaccg gaggcattta tccgccagac ttccagacca gttattgtgc cggagatgaa
     3121 aaggttttca ccttccttca ggatgttctg atggaggtta ttgaactctt tccatcctct
     3181 tacatccata taggcgggga tgaactggat aaaaaatttt ggcaagagtg cacggcatgt
     3241 aaaaagagga tgaaggatga agggctaaaa aatgtcgatg agctgcaaag ttactttata
     3301 cgtcgaattg agcaattcct gcaaacaaaa ggtcggagaa ttattggatg ggatgagatt
     3361 ctggagggtg gacttgcgcc ggatgccaca gtgatgagct ggcgaggtga aagtggaggc
     3421 atagcggctg ccgagatggg ccatgatgct attatgaccc ccggaacacc gctctatctc
     3481 aaccactatc aggcaggccc ggagggtgag cctctcgcca atggcggatt caacacgctg
     3541 aagatggtgt atgactatga tcctgtacca gcttcattaa aatcggaagc agccaggcac
     3601 atcctgggcg ctcagggcaa tatttgggga gaattcattc agacgcgctc ccatgtggaa
     3661 tatatggtat taccaaggat ggcagcactt gctgaagtag tttggagtcc agcatcacaa
     3721 aaggatttta cggacttttc gaaacgactc atctctcact tcaactatta tgagggtaaa
     3781 ggatacaatt attgcaaagg caattttacc gttgctatca gaccgatttc agataagggg
     3841 cgactctctg tggaattgac cagcgaggtt gcaggatgca gtattcatta tactacagat
     3901 ggttcgatgc cgggaccgga aagtccggaa tacaaatctc cgatccctat caccggctct
     3961 tgcctgctgc atgccgtgac agtactcgat ggcaaggtaa tgaacaccat tcccgccact
     4021 caggcttttg aatttcatga tgccatcggc gcaaatgtca attatgaatt tccctatagc
     4081 aattcttaca aggcagaagg ccccaatatc ctcaccgacg gaattcgggg aggtagcaat
     4141 atccataaat actggcacgg attcctcggc aaggaccttg tagctacagt aacactgccc
     4201 aattctatac atgtaaataa attatcactc agctgtatgc agcattataa agactggata
     4261 ttcttaccta cgcaagtaga ttatgaaatt tccgaagatg gcatacattt tgttggtgta
     4321 ggaaacgtga aaaacgatat tccggtaacg gaaacacaac agaccattaa ggaatttgcc
     4381 ttaaacctaa agcccgtaat ggcaagatat gtccgggtca aggctcatat cctgccggga
     4441 gcgcctgaag gacatcccgg aaagggcaat ccggtctgga tttttgcaga tgaattaaaa
     4501 gtaaattaac gatatagtca tgatcagttc gttggaaaaa atccccgtaa gtattttcaa
     4561 atcccctctg gaaggctcaa tatatgcagc ccagcagatc gccaacctta tccgtgccag
     4621 gcagatggaa ggcagaaatt gcgtacttgg tctggcgaca ggttccacac cgatttccat
     4681 gtacaaggaa ttagttagga tgcaccgaga ggaaggcctg agtttccgca atgtcatcac
     4741 tttcaacctg gatgaatatt atccattgga gagggatgcc atacaaagct actggagctt
     4801 tatgcacaac aacttattca ac
//
LOCUS       DAOQMC010000174         4793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8347_length_4793_cov_5.581469, whole genome shotgun sequence.
ACCESSION   DAOQMC010000174 DAOQMC010000000
VERSION     DAOQMC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8347_length_4793_cov_5.581469"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKV31_07490"
     CDS             complement(<1..369)
                     /locus_tag="PKV31_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92738.1"
                     /translation="MAGLDQFIIPVKGLHLGMHNYRFEVDDGFFKEKQSTVVESGKFE
                     VNVELDKHIDLMVLNISFSGKWRTSCDRCTADIAIPVDGQSEYLIKYATEESDDGDVI
                     YIMRESSEINVSDLILDTITV"
     gene            complement(471..1154)
                     /locus_tag="PKV31_07495"
     CDS             complement(471..1154)
                     /locus_tag="PKV31_07495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB92739.1"
                     /translation="MRQLVILVFSIGYIVLSGVSCKGDASVQEHSGFPDPVPNDSVTT
                     MTESDNEPDRVIWQKPDMILDLLGNLSDKVVADIGAGTGFFTFRLLQHAGKVIAVDID
                     KVPLDRMSQLSRKLDTAISNKLEIKLAKPNDPELKPASVDVIFMSNTYMYIQNRVPYL
                     KNLIQYLKPHARILIIDYKKKSIPFGPPIEERVDLGTVEEELMKAGYKIIKSDDVSLD
                     YQYIVLAER"
     gene            1718..3001
                     /locus_tag="PKV31_07500"
     CDS             1718..3001
                     /locus_tag="PKV31_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNB92740.1"
                     /translation="MEIWIILGLMVLNGLFAMCEIALASSKKARLIERASKGNKNAVI
                     ALKLTENPDSFLAAMQVGVTTIGILAGVYGGQAFAKDLETYFSQISWLSTYAHSLALL
                     IIIGGITYLTIVVGELIPKKIGLMYSEEITLFMAPFVRVFTKIAYPIVWFLTTSTKLL
                     SKLLFIKDVETDIVSEDELKMMVKMANEDGTIDNKESELIHNIFRFADRKAYSIMTPR
                     NEVVWIDANESSAEIHSDIIESGLTKFPVCDDSLDNILGIVNLKDYLISRNRPGFDIK
                     NILQPVVLIPETLTSLKILEEFRKKKRYFAVVMNEHGYVEGVLTLHDLTENIFGSLPD
                     LEEEEEIMIFKREDGSYLIDGSCQIDELREQIYIDEFFSEEADYTTIAGFVIEKLGKM
                     PEIGDKFSNDSYSFEIVDLDRTKIDKVLVTRISEE"
     gene            complement(3113..4291)
                     /locus_tag="PKV31_07505"
     CDS             complement(3113..4291)
                     /locus_tag="PKV31_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNB92741.1"
                     /translation="MSSKYNLRGVSADKEDVHAAIRSLDKGLYPSAFCKILPDLVGND
                     PDFCNIMHADTAGTKTSLAYMYWKETGDISVWKGIVQDAIVMNTDDMACVGCVDDIVL
                     SSTIGRNKALIGKEVLSALIDATSEFIDNMKNLDVNIYLSGGETADVGDIVRTADVGF
                     TAFARMKRSDLIVNRIRPGQVIVGLASYGKASYETEYNSGIGSNGLTMARHELFGGDY
                     VDRYPETFAPQTNREYVYSGKGRLTDEINIAGQSYPLGKLALSPTRTFLPVLKLIFKD
                     FRASIHGIIHNTGGGHSKVLKYCDSPVQIVKDHLLDIPPIFEIISKNAGVDWEEMFKV
                     FNMGTRLEIYTDEETALEIIRISNLFDIEAGVIGRVEEVRNEVSRVVVSHEDRKLIYQ
                     "
BASE COUNT         1484 a          994 c          890 g         1425 t
ORIGIN      
        1 aaccgtgatg gtatctagaa tcaaatccga aacattgatt tcggaggatt ctctcatgat
       61 ataaattaca tcgccgtcat cagattcttc ggtggcatac ttgatgagat actccgattg
      121 cccatccacc ggtatcgcaa tatctgccgt gcatctgtcg caggaagtcc gccacttacc
      181 ggaaaacgat atattcaaga ccatcagatc gatgtgcttg tccagttcaa cattaacttc
      241 aaatttgccg gattctacaa ccgtactttg cttctcctta aaaaacccat catccacctc
      301 gaaacggtag ttatgcattc ccagatgcaa gcctttaact ggtatgataa actggtctaa
      361 accggccatt cctattcata cttttacaaa tggactgcaa aggtatgttt aatttttgat
      421 ttcaaataat gtttttaaca cattttttaa aggtcaaccg aattcaaaag ttatctttcg
      481 gcaagtacta tatactggta atccagtgac acatcatcag acttgatgat cttgtagccg
      541 gccttcatca actcttcttc caccgtaccc aggtcaaccc gttcttcgat aggtggacca
      601 aatgggatgg atttcttttt atagtcaata atcaggatac gggcatgggg ctttaaatat
      661 tgaatcagat tctttaaata aggcaccctg ttttgaatat acatataggt attagacatg
      721 aagattacgt ctacacttgc aggctttagt tcaggatcat taggtttggc aagtttaatt
      781 tccaatttgt tggaaatagc agtatccagc tttcttgaca gctgactcat cctatcgaga
      841 ggcaccttat ctatatcaac agcaattacc ttgccggcat gctgaagcaa cctaaaggta
      901 aagaatccgg tacctgcgcc aatatcagcg acgaccttat cacttagatt gcccaataaa
      961 tccagaatca tatccggttt ttgccagatt actctatccg gttcattgtc tgattctgtc
     1021 atagtagtga cgctatcatt tggtacagga tcaggaaaac cggaatgttc ctgtacggat
     1081 gcatcccctt tgcaactgac gccggacaag acaatatatc caatagaaaa gaccaatatg
     1141 actaattgtc gcatcactca atatttaact gcaagtataa acaaaaaatg tagcaaaaga
     1201 ttaaaataac agtcaaaaag cccattgttg agttacacat ttatcagaag tctatttaac
     1261 caaaacataa ttctactcca ctgctaacag tattttgggc attacttttt gatatatata
     1321 tcgcctgatc gcagcaactg tgcgtgaaag ggtctgaagt aacataaccg gagaatatta
     1381 taacagctat aaacagctta tgattaatat attgctttta tttaatatat aaaaatctat
     1441 aaacctgtct ttacaattta atctccaatt atctcagaaa ttttaagaat acattttata
     1501 tagctatgcc ataaatccaa acaaaatgca tccatgtcaa ttttcacaat catcaagaac
     1561 agaaatatac gcttcacaca atgatgtcta tccatgatgc tgtcaaaaga ttgtgtcatt
     1621 gaaaataaat atatgattaa agcaaaatag ttacctttgc gctgaaaggc atgtagtatg
     1681 tgataaatgt aagaaaggcc tttgtcaaca cttttaaatg gaaatttgga ttattcttgg
     1741 attgatggtt cttaatggcc tgtttgccat gtgtgagatc gcattggcct catccaaaaa
     1801 agcaagatta atagaaagag cttcaaaggg gaacaaaaat gctgtcatcg ctttaaagct
     1861 tactgaaaat cctgatagct ttcttgctgc catgcaggtt ggagtgacca ctattggaat
     1921 tttggcaggt gtatatggtg ggcaggcatt tgcaaaggat ttggaaactt atttttcgca
     1981 aatatcctgg ttgagtacct atgctcacag tctggcatta ctaatcatca tcggtggaat
     2041 cacctacctc actattgtgg taggagaatt aattccaaag aagatcggac ttatgtattc
     2101 cgaagagatt accttgttta tggcgccctt tgttcgggtc ttcactaaaa tcgcttatcc
     2161 tattgtctgg tttttaacca cttcaaccaa acttttatcc aagctgcttt ttatcaagga
     2221 tgtggaaacc gacatcgttt cggaagatga gctcaagatg atggtaaaaa tggccaatga
     2281 agatggtacc attgataaca aagagagtga gcttatccat aatatattca ggtttgccga
     2341 cagaaaagcc tacagtatca tgacacctcg taatgaagtt gtctggatcg atgccaacga
     2401 atcttctgct gagattcact ccgacattat cgaaagcggc ctcaccaagt ttcccgtttg
     2461 tgacgacagt ttggataata ttctgggcat tgtcaaccta aaagattacc taatcagccg
     2521 gaacagacca ggttttgaca tcaagaatat attgcaaccg gtagtattga taccagagac
     2581 gctcacctca ctgaaaatat tggaagaatt cagaaagaaa aaaaggtatt tcgctgttgt
     2641 catgaacgaa catggctatg ttgaaggtgt gctgacccta catgatctca ccgaaaacat
     2701 tttcggcagc ttaccggatc tggaggagga ggaagaaata atgatcttta agagagaaga
     2761 tggttcgtat ttgattgatg gatcttgcca gatagatgag ctgagggaac aaatatacat
     2821 tgatgaattt ttcagtgaag aggcagatta taccaccatt gcgggctttg tgatagagaa
     2881 attaggcaaa atgcctgaga taggcgacaa attctccaat gacagttata gctttgaaat
     2941 agtggatttg gacagaacca agattgataa ggtcttagta acacggatat cggaggaata
     3001 aacgcgcctg gtgtctcgga aagttcttaa aaggaaagtt attggccgga ataacgcagt
     3061 aagaaacaca tcttaccggt cagatttaaa tggattacac aaacctatta actcattgat
     3121 agattaattt ccggtcttca tgtgacacaa caaccctaga aacctcattt ctcacctcct
     3181 ctaccctgcc gattactccc gcttcaatat caaacaggtt tgatatccga atgatttcca
     3241 aggcagtctc ttcgtccgta taaatttcca gtcgagtgcc catgttgaat accttaaaca
     3301 tttcttccca atcaacaccg gcatttttac tgatgatttc aaaaatcgga gggatgtcca
     3361 gcaagtgatc cttcacaatc tggacagggc tatcacaata tttgagtact ttggaatgac
     3421 ctccacccgt attatggatg ataccatgga tgctggcccg aaaatcttta aaaattagtt
     3481 ttagaacagg aagaaaggtc ctggtgggtg aaagtgcgag cttacccaaa ggatacgact
     3541 gtccggcaat atttatttca tctgtcaacc tgcctttacc gctatacaca tactctctat
     3601 tagtctgagg ggcaaaggtt tccggatacc tatctacata gtccccaccg aagagttcat
     3661 gtcttgccat ggtcaggccg ttagagccaa ttccactatt gtattcggtc tcgtaagaag
     3721 ccttgccata ggaggccaat ccgacgatta cttgaccggg acggattcta ttgacgatca
     3781 aatccgacct tttcattctg gcgaatgcag taaatccaac atcggctgtc ctaacaatgt
     3841 ctcctacatc tgccgtttcg cccccggaca agtatatatt gacgtccaga tttttcatat
     3901 tatcaatgaa ttcagaggtg gcatcaatca gagctgataa aacttcctta ccaattaagg
     3961 ccttattccg cccaattgtg ctggacaaga caatgtcatc cacacaccca acacaagcca
     4021 tatcgtctgt attcatcact atagcgtcct gaacaatgcc cttccagact gagatgtcac
     4081 cggtctcttt ccagtacatg tatgccagag atgttttggt gccggctgta tcagcatgca
     4141 tgatattgca aaaatccgga tcattgccta ccaaatcagg aagaatcttg caaaaagcac
     4201 tggggtacaa acctttgtcc aagctgcgaa ttgcggcatg aacatcttct ttatcagcgg
     4261 atactcccct caaattatac tttgatgaca taaaaaatac aaattatata ttatagataa
     4321 aaataatatg ttatctttgc gtcaaagaaa tcttaaatta caatattgaa ttgcccgatt
     4381 atgaactact tcgcgtcatt tttccgttga aaatacaaca attattatcc taaaacctat
     4441 ttatcatgta taggtcaatg tatcaattat cacaggcaaa ggtgatgaaa aaaaatgcaa
     4501 ttcttgcgtt tattctattt ttagtaatat tatctttctt gcttctctcg acgaaacaga
     4561 taccgagcag gaataatatt gccggtccgg ccactgctat caacgcaaca ccttaattat
     4621 gaaaacagac cgttcaactc accatcaatc atttcaatga ttgtacccag gtcttcttta
     4681 ttttgaataa agttcaacct gtcggtattg acgatcaata gtggtccgtg gttgtaggtc
     4741 tcgatccatt cttcatatcg ttgattgagt tttttcagat agtcaagact cat
//
LOCUS       DAOQMC010000175         4771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8401_length_4771_cov_4.985369, whole genome shotgun sequence.
ACCESSION   DAOQMC010000175 DAOQMC010000000
VERSION     DAOQMC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8401_length_4771_cov_4.985369"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1018)
                     /locus_tag="PKV31_07510"
     CDS             complement(116..1018)
                     /locus_tag="PKV31_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB92742.1"
                     /translation="MDDQISTVLMEVNELLRKVRKVELKSRDMSRLLFSGDTLSFFKG
                     RGMSFAEVREYQYSDDVRFIDWNVTARTGVPFVKVYEEERELNVIIMVDVSKSSFFGT
                     GTRLKSELITEMSAVLAFSAVQNNDRVGLILFSDKIELYIPPSKGRNQILRIIREMVN
                     FRPENKNTDIRKALRFLNTVIKKNSICFMLSDFQDDLFGAQMRIAGRKHDLIAIHIED
                     KAEHQLPDVGMLIAVDPETNEEFVLDTSDPAFRTAYQKQFEARKKKLQKIFGESKVDQ
                     IEITMGEDENFVRKLLYFFKSRAR"
     gene            complement(991..2283)
                     /locus_tag="PKV31_07515"
     CDS             complement(991..2283)
                     /locus_tag="PKV31_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92743.1"
                     /translation="MRFLVILFCAFICLPGYAQLTSCKDLWEGEASDRQYVISGWVDG
                     THTFRMFLCRKGDEVRGYYEMISAKKNTDVEGFFKGDSIFLAEYSKNQKVLGMIRGIF
                     AGTVFTADLSSPSGVYSGKMEGLTVGSWVEAKTPEPIDNPAIVVFTGVANPEETIVLS
                     FPNDYSCKGILHNTDLKKTYHMSGKRPDNTERKYNLKSYSLPQDGANMTCILNDEILI
                     QSTLPGKTSAMSAGIKIPLALIEKSDRLVSFEIHYPVIGTKGKENKLNIMVNDIRYEF
                     DSLFDIHLKNIHGEERPYSRAGTMVAWFEPSYVSGQWLCGHFIIRNNERNTSHIMPVN
                     YNYKKNIMVNIEDLISSPNDIGKQWNSIIPKQPEASDFSAKDASYGAPVLAFRGIVLS
                     TEYDPIFDRQLFQTNKKIKGVRWKWWKPEYWMIKFQQF"
     gene            complement(2311..3309)
                     /locus_tag="PKV31_07520"
     CDS             complement(2311..3309)
                     /locus_tag="PKV31_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB92744.1"
                     /translation="MTQYSEIEALNERVYKSSEFVSHLSDATANIIVGQEHMIERLLI
                     GLITKGHILLEGLPGLAKTLAIKTLATLVNGKFSRIQFTPDLLPADIVGTMIYNPTLN
                     KFSVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGEETFALEEPFLVMATQ
                     NPIEQEGTYPLPEAQLDRFMLKVTIDYPTKEEEQHIIRRHMDDSIGQLQQPVATTQEI
                     LDARAIIKSIYLDPSLERYILDIVFATRDVKSAGLPDLAPMIQYGASPRASINLALAS
                     KALAFIRRRAYVIPDDVRAIAMDVLRHRIGITYEAEAEGLTGESIITSILNIVNVP"
     gene            4073..>4771
                     /locus_tag="PKV31_07525"
     CDS             4073..>4771
                     /locus_tag="PKV31_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92745.1"
                     /translation="MTPKFLFLLILFFIIASVGTESMHAQHRKVTFHGTITDDATGET
                     LIGAALTIFPIKVSTVSNEYGYYSITFSESDSVTLVATYVGMEPLLLRLPVDKDLTVD
                     IRMKSGSVALDEVVISTDPLQEKMQGTQMGIEQISAKTSKVLPALFGEVDIIKTLQLK
                     VGVSSGSEGSAGLYVRGGAGDQNLILLDEATVYNANHLFGFFSTFNSDAIKDIRMYKG
                     GFPARYGGRLSSVID"
BASE COUNT         1282 a         1040 c         1031 g         1418 t
ORIGIN      
        1 cccagcaaga tgctgttggt gtcggccgtt acctccattt ggatttgggc tgataccgaa
       61 agtgccgaaa acgacatcag gcagaaaacc aagacatgtt ttatcatctg ttcaatcatc
      121 gcgcccttga cttaaagaag tacaacaatt tacgcacaaa gttctcatcc tcacccatgg
      181 tgatttcaat ctgatcgacc ttactctcgc caaaaatttt ttgtagtttt ttctttcttg
      241 cctcaaattg cttttgatag gctgttctaa aggccggatc cgatgtatcg agaacaaatt
      301 cttcattggt ctccggatcc acggcaatca gcataccgac atccggaagt tgatgctctg
      361 ccttgtcttc gatatggatg gcgatgaggt catgcttgcg tcctgctatg cgcatctggg
      421 cgccaaaaag atcatcctga aagtctgaca gcataaagca gatgctgttt ttctttatta
      481 cagtattcag gaagcggagt gcctttctga tgtcggtatt cttgttttca ggtcgaaaat
      541 ttaccatttc ccggatgatg cgcagaattt ggttgcgtcc tttggagggg ggaatataca
      601 attcgatttt atcggaaaac aaaatcaggc cgactctgtc gttgttttgt accgctgaaa
      661 aagctaatac agcactcatc tcggtaatca gttcgctttt aaggcgtgta ccggtcccga
      721 aaaaggacga cttgctaaca tcgaccataa tgatcacatt caattcacgt tcctcctcat
      781 agaccttaac aaaaggtaca ccggtacgtg cagtaacgtt ccagtcaata aaccggacat
      841 cgtcactgta ttggtattcg cgcacttccg caaatgacat gccgcgtcct ttgaaaaagc
      901 tgagggtatc acccgagaaa agaagccgcg acatatccct cgacttcagc tctactttcc
      961 tgactttacg caacaactca ttgacttcca tcagaactgt tgaaatttga tcatccaata
     1021 ttcaggtttc caccatttcc atctaacgcc tttgattttt ttgttggtct gaaacaattg
     1081 cctgtcgaaa ataggatcat attccgtact gagcactatt ccgcgaaagg ccaataccgg
     1141 ggcgccgtat gatgcatctt tagctgaaaa atcacttgcc tcaggttgct tggggatgat
     1201 tgaattccat tgtttgccga tgtcattggg ggaagatatc aggtcttcaa tattgaccat
     1261 gatatttttt ttgtagttat aattgaccgg catgatatga gaagtattcc tttcattatt
     1321 cctgatgatg aagtgaccac atagccactg accagacacg tacgatggtt caaaccaagc
     1381 taccatggtg cctgcccggg aataaggcct ttcttctcca tgaatatttt tcaggtgtat
     1441 atcgaacaag gagtcaaact catacctgat atcgttgacc attatattca atttgttttc
     1501 cttgcctttt gtgccgatga ccgggtaatg tatctcaaat gaaaccaatc tgtctgattt
     1561 ttctataagg gctaatggaa tctttatccc tgcagacatt gcggaagttt tgccgggcaa
     1621 agtactttga ataaggattt cgtcattgag tatacaggtc atgtttgctc catcttgcgg
     1681 cagggagtag cttttcaggt tgtattttct ttctgtgtta tcagggcgtt tccctgacat
     1741 gtgataagtt ttttttaagt cggtattatg gaggattccc ttacaggaat agtcattggg
     1801 aaaggaaaga acgatggttt cttccggatt ggcaacaccg gtaaaaacta caattgcagg
     1861 gttatctatg ggctcggggg tctttgcttc cacccaggat cccactgtca atccttccat
     1921 tttgccggaa tagactccgg atggggaaga aagatctgct gtaaatacgg tgccggcaaa
     1981 tattcctcga atcataccta ataccttttg attcttgcta tattctgcaa gaaatatgga
     2041 atcacccttg aaaaagccct ccacatcagt gtttttcttg gcagagatca tctcatagta
     2101 tccccgcacc tcgtcacctt tcctacacag aaacatgcgg aaggtgtggg ttccatccac
     2161 ccatccgctg ataacgtatt gccgatcgct tgcttcccct tcccaaagat ctttacagct
     2221 ggtcaattga gcatagcccg gcaagcaaat gaaagcacaa aacaaaataa caaggaaccg
     2281 catcactact atttaatgta tgaaagtgca tcaaggcaca ttgacgatat taaggatgga
     2341 tgtgatgatg gattcaccgg tgaggccctc tgcttcagcc tcgtaagtaa tgcctatcct
     2401 gtgtcttaag acatccatgg ctatcgcccg cacatcatcc ggaatcacat aggcccgacg
     2461 tctgatgaag gccagagcct tggatgccaa tgccagatta atactcgccc ggggagaagc
     2521 gccatattgg atcatgggcg ccagatcggg cagtccggca gactttacat cccttgtagc
     2581 gaatacgatg tcgagtatat atctttccaa agacgggtcg agatagatgg atttgattat
     2641 agctctggca tctaaaattt cctgtgttgt agccactggt tgttggagtt gtccgattga
     2701 atcatccatg tgtcgacgga tgatatgttg ctcttcctcc ttggtgggat agtcgattgt
     2761 aacctttagc atgaatcggt ctagctgggc ttcgggcaat ggataagtcc cttcttgttc
     2821 gatgggattc tgggtagcca taaccagaaa aggttcttcg agggcaaagg tctcctcgcc
     2881 gatagtgacc tgcctctcct gcatcgcctc caatagtgca ctttgcacct ttgccggtgc
     2941 acggttgatt tcatcagcca gaataaaatt ggcaaatatc gggcctttct ttacggagaa
     3001 tttgttgaga gtgggattgt aaatcatggt accgacaatg tctgccggca gtaggtctgg
     3061 agtgaattga atccttgaaa atttgccgtt aaccaaggtg gcgagggtct taatggcgag
     3121 ggttttggcc agtccaggca agccctccaa caggatgtga cctttggtga tcaggccgat
     3181 taaaagcctt tcgatcatgt gttcctgacc gacgatgata ttggcagtgg cgtcagacag
     3241 gtgggataca aattcactac ttttgtacac ccgctcattc agcgcttcta tctcagaata
     3301 ttgcgtcata aatatcgttt taatttcaat aaaagtttcg aatccatgct tttattggca
     3361 acctctgttg tcaggttcca agcatgtcaa ccgtataaac tatttaagaa caaaaatagt
     3421 atttcaaagg tcggaaaatt cccgcaattg gccgaatttg acaatttgtt aaccgcgttt
     3481 ttattgtaaa actcctgtaa gagtataatt tggtgaattg atcaaaaggt agaatagcat
     3541 tgatcccagg taaataaaca ggaatttaga aaacaccctc ccaggacgcc gggaattacc
     3601 ggcatcgcat ggtaggctgt aaaataataa ggtttattgt gagtgattgg aaaaatattg
     3661 gacgatttgc tctaaagtat gttttaaaca ggaaatcggg taatgaatat cagcagaaac
     3721 aaaacgatca acatgatagc ccagattctg acatccttaa acatttcatg taacaccact
     3781 ttaacttctt ttgcaacagg tttcatattc tctctttttc atcaaaaacc acaacataaa
     3841 tcttacaaaa acgataccat aacaataatc tataatattt acttcggaag tcgcttggta
     3901 attatcttat tataaatgtt tcgatgtttg ccgaaggcca tattaaaata atttaaattc
     3961 gtttgtctta tgtatttttg tccttcggat tcgttatttt gaaaaaccaa ggcttttaac
     4021 ttttcatccg gcaaatgagc atctccatcc taagtatctt ttaatcgata agatgacccc
     4081 aaaattcttg tttttactca ttctgttttt tattattgct tcagtgggca ccgaatcaat
     4141 gcatgcacag catagaaagg ttaccttcca tggtaccatt accgatgatg cgacaggaga
     4201 gacgctaatc ggtgccgctt taacaatctt ccccattaaa gtatcgacgg tgtccaatga
     4261 atatggttac tattcgatta ctttttctga aagtgattca gtgacactgg ttgccaccta
     4321 tgtagggatg gagcctttgt tgctaaggct tcctgtcgat aaggacttaa cagtcgatat
     4381 acgcatgaaa tcaggttctg tcgcacttga tgaagtcgtc atctcgactg atcccctaca
     4441 ggagaagatg cagggcacac agatgggtat tgagcagata tcagctaaga ccagcaaggt
     4501 attgcccgca ctatttggag aggtggacat catcaagacc ctccaactga aagtcggtgt
     4561 ctcatccggt tcagagggga gtgcagggct gtatgtcaga ggaggtgccg gggaccagaa
     4621 tctgatctta ttggatgagg ccactgtata caatgccaac catcttttcg gttttttctc
     4681 caccttcaat tctgatgcca tcaaagatat ccggatgtac aagggtggtt ttccggcgcg
     4741 gtatggtggc aggctatcat ctgtgattga t
//
LOCUS       DAOQMC010000176         4763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8419_length_4763_cov_4.847706, whole genome shotgun sequence.
ACCESSION   DAOQMC010000176 DAOQMC010000000
VERSION     DAOQMC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8419_length_4763_cov_4.847706"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PKV31_07530"
     CDS             complement(<1..580)
                     /locus_tag="PKV31_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HNB92746.1"
                     /translation="MEALQNIKRKFGILGVSATLDNALNTAIRVAPTDLTVLISGESG
                     VGKDVFSRVIHSLSPRKHNPFIAINCGAIPEGTINSELFGHEKGSFTGALGDRKGYFE
                     TVHGGTIFLDEIGEMPLDTQAFLLRVLESGEFIRVGSSKTLKTDVRVIAATNVNLEEL
                     IRKGKFREDLYFRLSIVPIKVPALKERREDIKI"
     gene            complement(649..2076)
                     /gene="miaB"
                     /locus_tag="PKV31_07535"
     CDS             complement(649..2076)
                     /gene="miaB"
                     /locus_tag="PKV31_07535"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNB92747.1"
                     /translation="MRTQELLDRPHSVDVPEEMQGSVYLQHGITYGSKKFYIESYGCQ
                     MNFSDSEIVASILSKEGYGSTADIYEADLVLINTCSIREKAEDTIRKRLEYYRQIKKT
                     NKSMMVGVLGCMAERMKKTLLEEEKLVDIVVGPDAYRDLPNLIAGASEGERQINVLLS
                     REETYADISPVRLDSNGVSAFISIMRGCNNMCSFCVVPFTRGRERSRDPYSIVSEARD
                     LFENGYREVTLLGQNVDSFNWKSMDGNEQVNFAQLLEMVAQVDPLLRIRFSTSHPKDI
                     TDDVLYTMAKYENICKYIHLPVQSGSSRILTLMNRTYDADWYRSKIDRIYEIMPECAI
                     SSDIIAGFCSETDDEHRESIAMIDYARYTMSYMFYYSERPGTPAAKKLVDDVPMEIKI
                     SRLNEIIEKQRQISAEANKSDEGKEFKILIDGYSKKSESQMKGRTSQNKVVVFDIHPD
                     YKIGDYAKVYITGSTTATLFGEIIH"
     gene            complement(2237..3469)
                     /locus_tag="PKV31_07540"
     CDS             complement(2237..3469)
                     /locus_tag="PKV31_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNB92748.1"
                     /translation="MRKLIILLVLSILCFGFITEKNNYEDWGFFGHRRINRYAVFTLP
                     QEMLPLFKGNIDFLTEHAVDPDKRRYAFRLEAPRHFLDMDRYKDACLPENFVEAFSAF
                     AMLKVITHTNDTLLIHSSDTSWCNNLPNAVMTDGSNVWNIGKKELRHHFRQFILPAYY
                     DLRYQINPDSIRPLLPTHIQAIKNISLIDSFTIHGIVPYIIEQQYDKLVRAFKKLDKN
                     EIINIAADLGHYIADAHVPLHACSNYNGQKTDQYGIHAFWESRIPELFADTQYDFLVG
                     KATYIRDVRKFAWETIRTSGSLADSVLSMEAALKSFYPRDQQMCFDQRLGQTVWIQCR
                     DYAAAFQDKMQGMVEIRMREAIHSVGSLWYSAWVEAGSPPLNFSNDIKAAWKIDSTVL
                     EYDNIIKKGGDGYGRNHE"
     gene            complement(3466..4467)
                     /locus_tag="PKV31_07545"
     CDS             complement(3466..4467)
                     /locus_tag="PKV31_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92749.1"
                     /translation="MSSKLKYIIGLFLSLLIVTGIVYFNKSGKSSMADSSRDFAIEDI
                     EDIGKIFVTDREGNHYSLDRKGNVWYADSVYLADPGFVDVVLTTLKRMTVVNLVPKPA
                     IKNVMRDIATLGKKVEIYDRKGRKLRSFYIGGTNQRVTASYMVLEGYNIPYEMSYPGF
                     SGDLGGRLWPLHLIDIRSKDIFRYKAGDIKKITVTYPRDKNESFTLTISNSNKYDIEP
                     LSQTVTPIAKPISKGAVEQYLSAFENIQAAKVVEKNLLNADTMVSSVPFATFEIVDRN
                     DNIRKLDLYPIMDESARVGLGSEPEFFFAILDNKNFFNCQLIVMKKLFWGYSFFFDR"
     gene            complement(4486..>4763)
                     /locus_tag="PKV31_07550"
     CDS             complement(4486..>4763)
                     /locus_tag="PKV31_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92750.1"
                     /translation="ENNEYMPMGYNTFDKILYGNKDFVINAVEYMFDESGILSARAKE
                     VKLRLLDNVRIKEEKTYWQMLNVLLPISLLVLGTFVFNYIRRKRFAS"
BASE COUNT         1333 a         1004 c          903 g         1523 t
ORIGIN      
        1 ggatcttgat atcttcacgt ctttccttca acgccggcac ctttatgggc acaatggaaa
       61 gtctgaaata aaggtcttct ctaaacttcc cttttctgat caattcttcc agattgacgt
      121 tggtggcagc aattaccctg acgtcagttt ttagggtttt ggacgaacca accctgatga
      181 attcaccgga ttcgaggact cgcagaagaa aggcctgcgt atcaagtggc atctctccta
      241 tttcgtccag aaatatcgtt ccaccatgga ctgtttcaaa atatcctttt ctgtcaccca
      301 aagcacctgt aaatgagcct ttctcatggc caaataattc actattgata gttccttcgg
      361 gaatggcacc acagttgatg gcaataaagg gattatgctt ccttggactt aatgaatgaa
      421 ttaccctgga aaaaacatcc ttacctaccc cgctctctcc tgatatcaga acagtcagat
      481 ctgtaggcgc tacacgtatg gctgtgttca atgcgttgtc caatgtggcg gaaacgccaa
      541 gaatgccaaa ctttcgtttt atgttttgca gtgcctccat ggatttatct ttattaaaaa
      601 attcaaatga agcgtttaca agtcaatttt ggtataaaat atttattttc agtgaattat
      661 ttcgccaaat agagtcgctg tcgtactacc ggtaatgtat actttggcat agtcacctat
      721 cttataatcc gggtgaatat cgaaaacaac cactttattt tggctggtcc gtcctttcat
      781 ctgggactcg gactttttag agtatccatc gatgaggatt ttaaattcct taccttcatc
      841 cgatttgtta gcctcagcac ttatctgtct ttgcttttcg attatttcat tcaaccgact
      901 tatttttatt tccattggta catcatcaac cagtttttta gccgcagggg ttcccggtct
      961 ttcactgtaa tagaacatgt aactcatcgt atatcgggca taatctatca tcgcgatgct
     1021 ttctctgtgt tcatcgtcgg tttctgaaca gaatcctgct atgatatcgc ttgaaattgc
     1081 acattcgggc atgatttcat atattctgtc tatcttagac ctgtaccagt ctgcatcata
     1141 ggtacggttc attaatgtga ggatacgact ggaaccgctt tgaacaggca ggtgaatata
     1201 cttacagata ttttcatatt ttgccatagt atagagtaca tcatccgtga tgtctttggg
     1261 atgtgatgtc gagaaccgaa tcctaagtaa aggatcaacc tgcgcgacca tttccaataa
     1321 ttgcgcaaaa ttgacctgct cgttaccatc catgcttttc cagttgaaag aatcgacatt
     1381 ttgaccgagt agagtcactt cacgataacc gttttcaaac aaatcccgtg cttctgatac
     1441 gatcgaatat ggatcccggc ttctttcccg ccctctggtg aatgggacta cacaaaacga
     1501 acacatgttg ttacaacccc gcatgatgct gataaatgca gaaactccgt tggaatccag
     1561 tcgtaccgga cttatatcgg cataggtttc ctcacgggaa agtaaaacat tgatttggcg
     1621 ctcgccttct gatgcgcctg cgattagatt tggcagatcc ctgtaagcat cagggccgac
     1681 aacgatgtct accagttttt cctcttccag cagggttttc ttcatccttt cagccataca
     1741 cccaaggaca ccaaccatca tggatttatt ggttttcttg atttgtctat aatattcaag
     1801 gcgtttgcgt atagtgtcct cggccttttc ccttatagag caggtattaa tcaataccag
     1861 atctgcttca taaatatctg ctgtcgatcc atatccttcc tttgagagaa tactggctac
     1921 tatttcagag tcagaaaaat tcatttgaca accatagctt tcgatataaa actttttgga
     1981 tccatatgta atcccatgtt gtaggtatac tgaaccctgc atttcctccg gcacatcaac
     2041 ggagtgtggt ctgtcaagta attcttgtgt acgcatgttt caaaattaaa ggcacaaagg
     2101 tattaagaaa atctgacatt tcgtcatgat atgacaaaag tacttatggt ccagaaatga
     2161 ataacagggt tggaaagttg tggttctaac tttggatgtc ggttaaacca atcaagccta
     2221 ttttggcttt ggcttattat tcatggttcc gaccgtaccc gtctccacct tttttgatga
     2281 tattatcata ttctaaaact gtggaatcga ttttccaggc agctttgatg tcatttgaaa
     2341 aattcaaagg tggtgaccct gcctctaccc aggctgaata ccagagcgaa ccaacggaat
     2401 gaatagcttc tctcattcgg atttcaacca taccctgcat tttatcctga aaagcggctg
     2461 cataatcacg acactgaatc cacactgttt gtcctaatcg ttggtcaaag cacatttgct
     2521 gatcccgggg atagaatgat ttcagtgcgg cttccattga taacacactg tcggcaagtg
     2581 acccgcttgt cctgatggtt tcccaggcaa acttgcggac atccctgata taagtcgcct
     2641 tgcccaccag gaaatcatat tgtgtatcag caaataattc cggaatgcgg ctttcccaaa
     2701 atgcatgaat accgtattgg tctgttttct gaccgttata attgctacag gcatgcagag
     2761 gtacgtgcgc atcagcaata tagtgtccta aatcggctgc aatgttgatg atttcatttt
     2821 tatccaattt cttgaatgca cgtactaatt tatcatactg ttgttcaata atataaggaa
     2881 cgatgccatg gatcgtgaag ctatcaatta gggaaatgtt tttaatagct tgaatatgag
     2941 tgggtaataa aggtctgatt gaatccggat tgatttgata acggaggtca taataggcag
     3001 gtaatataaa ttgacgaaag tgatgcctca actctttttt tccgatgttc catacattgc
     3061 tgccatctgt catgacggca tttgggaggt tgttgcacca gcttgtatct gatgagtgga
     3121 taagtaaggt atcgttagta tgggtaatga ccttcaacat tgcaaatgca gagaaggctt
     3181 cgacaaaatt ttccggaaga caggcatctt tgtacctgtc catatccaga aaatgtcgag
     3241 gtgcttccaa tcgaaaagca taccgtctct tgtcaggatc aacggcgtgt tctgtaagaa
     3301 aatcaatatt acctttaaaa agaggtaaca tctcttgtgg taatgtaaaa acagcgtatc
     3361 tattgattct gcgatgccca aagaaacccc agtcctcgta attatttttt tctgttataa
     3421 agccaaagca cagaatgctt aaaaccagaa gaattattaa ttttctcatc tgtcaaagaa
     3481 gaaagaatag ccccagaata actttttcat cacgattaat tgacagttga agaagttttt
     3541 attatccaat attgcaaaga agaattccgg ttctgaacca agtccgacac gtgcagactc
     3601 atccatgata ggataaagat cgagtttcct gatattatca tttctatcca ctatttcaaa
     3661 agtagcaaaa gggacgcttg ataccattgt atcagcattt agtaagtttt tttcaacgac
     3721 ttttgcagcc tgaatatttt caaacgcact gagatattgt tcgactgcac ctttactgat
     3781 aggctttgcg atgggggtaa ctgtttgact gagcggttca atatcatatt tgttggaatt
     3841 tgaaatggtc agggtgaaag attcattttt gtccctggga tatgttacgg ttattttctt
     3901 tatatctccg gctttatatc taaaaatgtc ttttgatcta atatcaatca aatgtagtgg
     3961 ccataatcta ccgccgaggt ctccgctgaa tcccggataa ctcatttcat agggaatatt
     4021 ataaccttcg agcaccatat aggaggcggt gactctttga tttgtcccac ctatatagaa
     4081 agatcttagt tttcgtcctt tccggtcata tatttccact tttttgccta aggtagctat
     4141 atcacgcatg acatttttga tggcaggttt tgggacaaga ttaaccactg tcattctttt
     4201 caaagtagta agaaccacat ccacaaatcc gggatcagcc agatatacgc tgtccgcata
     4261 ccaaacatta ccttttctat ccagggaata atggttgcct tccctatcag taacaaatat
     4321 tttaccgata tcttctatat cctcgattgc aaaatcacgg ctactgtcag ccatggagga
     4381 tttgccggac ttgttgaagt aaacgatgcc tgtaacgatc aatagtgata gaaaaaggcc
     4441 tatgatgtat tttaatttag aagacatatc aatggttctg aattattatg aagcgaatct
     4501 cttcctcctg atataattga atacaaaggt tccaagtaca aggagtgata ttggtaacaa
     4561 aacattcagc atttgccaat aggtcttttc ctccttgatt ctgacattat ccaatagacg
     4621 caatttcact tcctttgccc tggcagaaag gattcctgat tcatcaaaca tatattccac
     4681 ggcatttatt acaaaatctt tgttgccata taatatttta tcaaatgtgt tataacccat
     4741 cggcatatat tcattatttt cag
//
LOCUS       DAOQMC010000177         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8550_length_4721_cov_5.066867, whole genome shotgun sequence.
ACCESSION   DAOQMC010000177 DAOQMC010000000
VERSION     DAOQMC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8550_length_4721_cov_5.066867"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="PKV31_07555"
     CDS             <1..1599
                     /locus_tag="PKV31_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNB92751.1"
                     /translation="GDLALRTIGYARKNSQSIGLEASYDEYLKGEAGKRMVQYISPTK
                     YIPINDLTEIEPKNGDDIHTTINVQMQDIAGQALRTALDYHKASYGTVIIMEVKSGNI
                     KAMSNLKRDGGVFTEDYNYAAGLSTEPGSTFKLASVMAILEDKMMSLDKEFEINYGKS
                     IISGREMKDSEGHGHAKLSLRRAFELSSNIGISKAVVEAYGGSREGSFKFVDRIKQFH
                     LDKITGIDLPGEGKPLIKTPYNLKQLWSKSTLAWMAHGYETQLTPLQLLTFYNAVANN
                     GKMMQPRLVTGVWSHGEMVKDFQPKVLVDKIASDTTIMLARTLLEGVVLNGTARRYKN
                     DMYSFAGKTGTASLDYGGIKRGRYRASFIGYFPADNPMYSICVSITEPREHGYHGAEV
                     ALPVFRSVADKILSFDPKFFKVTSPEKNAMVVTDQLPQGEKGYRTDFLSIFNRLGVPV
                     YSGGGITDFIVTRKVGSDVNLMAMNEPPGLVPDVTGMGLRDALYALESRGLKAEVNGY
                     GRVSTQNISPGTKVSGQKVVIRLE"
     gene            1601..3058
                     /locus_tag="PKV31_07560"
     CDS             1601..3058
                     /locus_tag="PKV31_07560"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNB92752.1"
                     /translation="MPVIQLNVLLKSAGITHSSSGNLVEISNIAIDSRKVSKGSLFVA
                     IKGSEQDGHKYIRKAIDAGASAIVCQEKADVPDDILMVLVEDSADAAGRLASAFYGHP
                     SEALKLVGVTGTNGKTTTVTLLYQLMKQLGHRTGLISTVENIVNGRVVVATHTTPDPV
                     ELNALLAEMVAVGCEYAFMEVSSHAAHQKRISGLRFEGAVFTNITHDHLDYHKTFDDY
                     IAAKKSFFDGLASGSFALVNGDDRRSDIMLQNTKASKHFYSLRRSSEFKGKIFENTLE
                     GLEMEIDGLHAHLLLIGEFNAYNALCAYGICRLLGFEREVILTGLSALPGARGRFETV
                     RDMNRGVLYIVDYAHTPDALEKVLKTIEVMKQKGGIITVVGCGGHRDKEKRPKMGAIA
                     ASLSREAIFTDDNPRDENPADITAAMMDGVDDADRNKVLIINDRAQAIKTAAKLASRG
                     DIVLVAGKGHETYQEGMNGKIDFDDRRVIEDIIGR"
     gene            3288..4547
                     /gene="mraY"
                     /locus_tag="PKV31_07565"
     CDS             3288..4547
                     /gene="mraY"
                     /locus_tag="PKV31_07565"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNB92753.1"
                     /translation="MLYYLFEYLYKDLNIPGTGVFRFITFRAGLAIVLSLLISIVFGK
                     RIIQSLRRRQIGETVRDLGLEGQKEKEGTPTMGGIIIVLAIVVPCLLVARLDNVYIIL
                     MLISTLWMASIGYLDDYIKVVKKDKDGLKGVFKIIGQVGLGLLVAITMLMSNDVTVRM
                     TPQEAEQGEYKIVKTVMVEDPHNAGKLVEMVHAKAAITNVPFFKNNELDYGRLLDFMH
                     MNKEVMLWIVFIVAVVFIITAVSNAANLTDGIDGLATGVSATIGAVLAAFAYLSGNSV
                     FADYLNITFIPNSSELVIFSSCFIGACTGFLWHNSFPARVFMGDTGSLTLGGIIAALA
                     IVLRKELLLPILCGIFVVENLSVIIQRYYFKYTKKKYGEGRRVFLMAPIHHHFQKKGY
                     HEAKIVTRFWIVSIILAVMTILTLKIR"
BASE COUNT         1373 a          869 c         1209 g         1270 t
ORIGIN      
        1 ggtgaccttg cccttagaac tattggctat gcacgtaaga attctcagtc gattgggttg
       61 gaagcatcct atgatgaata tttgaaaggt gaggcaggaa aaaggatggt tcaatacatc
      121 tcaccaacga agtatattcc aataaatgat ctgacggaga ttgagcccaa gaatggcgat
      181 gatatccata ctaccattaa tgtccagatg caggatatag ccggacaagc actgaggact
      241 gcattagatt atcacaaagc ctcctatggg acagtaatca tcatggaggt caaatcaggc
      301 aatatcaagg ccatgagcaa tcttaagagg gatggtggag tctttaccga agattacaac
      361 tacgctgcag gattgagtac cgagcccggt tcgactttta agctcgcttc cgtgatggct
      421 attttggaag acaaaatgat gtccctggat aaggagtttg agattaatta cggtaaatcg
      481 atcatcagcg gccgtgaaat gaaggattcc gaaggtcatg gacatgctaa gctgtctctc
      541 agaagggcat ttgagctttc atccaatatt ggaattagca aggcggtggt tgaagcatat
      601 gggggcagta gggaaggttc cttcaaattt gtggacagaa ttaaacaatt ccatcttgac
      661 aaaataacgg gtattgatct tccgggcgaa ggaaaacctt taataaagac tccatataac
      721 ttgaagcagc tgtggagtaa gtcaacgctt gcatggatgg ctcacgggta cgaaacccag
      781 ctgactcctc ttcaattgct gaccttctat aacgctgttg cgaataatgg taagatgatg
      841 cagcctcgat tagtgacagg cgtctggtct cacggagaaa tggttaaaga ttttcaacct
      901 aaggtcttgg tcgacaaaat agcaagtgat acaacaatta tgcttgcacg gaccttgctg
      961 gaaggagtcg tcctcaatgg aactgcacga agatataaaa atgatatgta cagctttgca
     1021 ggcaagactg gcacagcaag cctggattat ggcggtatca agcgcggaag atatcgcgct
     1081 tcattcatag ggtattttcc ggcagataat ccaatgtatt ccatttgtgt atctattaca
     1141 gagccaaggg aacatggata tcacggtgcg gaagttgctt tgcctgtatt caggtcagtg
     1201 gcggataaga ttctttcatt tgacccgaag tttttcaagg taacttctcc tgaaaaaaat
     1261 gcaatggttg ttactgatca attacctcag ggggagaaag gatatcgcac cgacttttta
     1321 agcatattca accggttagg ggtccctgtt tattcgggag gaggaatcac agacttcatt
     1381 gtgaccagaa aggttggctc tgatgtgaat ctgatggcga tgaatgaacc gccgggtctg
     1441 gtgccggatg tcaccggaat gggtcttcgt gatgcattgt acgccctgga aagtcgggga
     1501 ttgaaggcag aagtgaatgg atacggaaga gtatcaactc aaaatatatc acccggaaca
     1561 aaggtttcgg ggcagaaagt agtaatcaga ttggaatgat atgccagtga tccagctcaa
     1621 tgttcttttg aagtcggcgg gcattaccca ttcttcatcc ggaaatttag tcgaaataag
     1681 caatatagcc atcgacagcc ggaaggtttc gaaagggtcg ttgttcgttg cgattaaagg
     1741 atctgagcag gatggtcata aatacatccg taaagcaatt gacgctggag cttcagcaat
     1801 agtttgccag gaaaaggcag atgtgcctga tgatatcctg atggtattgg ttgaagacag
     1861 tgcagatgct gcggggaggt tggcatctgc cttctacgga catccatcgg aagcactaaa
     1921 actggtggga gtaaccggaa ctaatggcaa aactaccacc gtcactttgc tttaccagtt
     1981 gatgaaacaa ttggggcatc ggacaggtct gatttctacg gttgaaaata tagtgaatgg
     2041 aagagtggtg gttgcgaccc atactacacc tgatccggta gagcttaatg ctcttttggc
     2101 tgaaatggtt gctgtcgggt gtgaatacgc cttcatggag gtgagttccc atgctgctca
     2161 tcagaagcgc attagtggtt tgagatttga aggtgctgta tttaccaata tcacccacga
     2221 tcatcttgat taccacaaga ccttcgatga ttatatagca gcgaagaaat cattttttga
     2281 tggcctggct tcaggaagtt ttgccctggt caatggggat gaccgtcgaa gcgatatcat
     2341 gctgcagaat acaaaggcat ctaagcattt ctatagtctc agaaggagct cagaatttaa
     2401 aggtaaaatc tttgagaata ccttggaagg tctggagatg gaaattgacg gattgcatgc
     2461 tcatctgctg ctgatagggg aattcaatgc ttataatgct ttatgcgctt acggcatatg
     2521 caggttactc gggtttgaaa gagaggtgat tcttacagga ttaagtgctc tcccgggagc
     2581 aagaggtaga tttgagacag tccgggacat gaatagggga gtattgtata tcgttgatta
     2641 tgcccataca ccggatgcgt tggagaaagt acttaagacc atcgaggtga tgaaacaaaa
     2701 gggcggaatc attacagtag tgggttgtgg aggtcatcgt gacaaagaga agcggcctaa
     2761 aatgggagca attgctgcca gcttaagtcg agaagccatc tttaccgatg ataacccaag
     2821 ggatgaaaat ccggcggaca ttactgcagc aatgatggac ggagtggatg atgccgacag
     2881 aaataaagtt ctgatcatca acgacagggc ccaggccatc aaaacagcgg ctaaactggc
     2941 atccaggggg gatatagtgc ttgtggcagg taaaggccat gaaacatatc aggaggggat
     3001 gaatgggaaa attgattttg acgaccgaag ggtcattgaa gatataatcg gcaggtaaag
     3061 ttctgtcgga aagacgtagg ttctattttt aaatttggtt ggaatgagca ggtcaggcaa
     3121 tccggaaact ggctgtcata acggttctaa tgccagcgca gactggttgc ccctgctcac
     3181 catttttatt tggaacatca ggttatgaag attgaggaaa accaaagaaa ccaagacaaa
     3241 gtgtatctga aaagctacac cgattaaaaa attattacat aggcgagatg ctgtattatt
     3301 tgtttgaata cttatacaaa gatctgaata tcccgggcac cggagtgttc agatttatca
     3361 cgtttcgagc gggtcttgct attgtattgt ctttattgat ttctatagtt tttggaaaaa
     3421 gaatcattca atcactccgg cgtcgtcaga ttggcgagac agttcgagat cttggtcttg
     3481 agggccaaaa agaaaaggaa ggaacaccaa ccatgggcgg tatcatcatt gtcctggcaa
     3541 ttgtagtgcc atgtctgctg gtggctcgcc ttgacaatgt ctatattatc ctgatgttaa
     3601 tttccacatt gtggatggca agcatcggtt acctggatga ttacattaag gtagtgaaga
     3661 aggacaaaga cggtttgaaa ggcgttttca agataattgg tcaggtcggc ttgggattat
     3721 tggttgcaat aaccatgctg atgagtaatg atgttaccgt aaggatgacc ccgcaagaag
     3781 cagaacaggg agaatataag atcgtaaaaa ccgtcatggt tgaggatccc cataatgccg
     3841 gtaagcttgt agaaatggta catgccaagg cagccattac aaatgttccc ttttttaaga
     3901 acaacgaatt ggactatgga aggctacttg atttcatgca tatgaacaag gaagtcatgc
     3961 tttggattgt attcatagtt gcagtggtat tcataatcac tgcggtcagc aatgcggcta
     4021 atctgacaga cggaattgac ggactggcta ccggtgtgtc cgctacgatt ggtgccgtac
     4081 tggctgcttt tgcctatttg agcggaaact ctgttttcgc ggattacctc aacatcactt
     4141 ttattcccaa tagtagtgaa ttagtaatat tcagttcatg ctttatcggt gcatgtaccg
     4201 gattcttatg gcataattca tttcctgccc gggtatttat gggtgataca ggcagcctga
     4261 cacttggcgg tatcatagca gctttagcta ttgtattgcg aaaagaactc ttgttgccga
     4321 tactttgcgg aatttttgtt gtcgagaatc tctctgtgat catccaaaga tattacttca
     4381 agtataccaa gaaaaaatat ggtgaaggca ggcgtgtctt tttaatggca cctatccatc
     4441 atcattttca gaagaaagga taccacgagg ctaaaattgt tacaagattt tggatcgtgt
     4501 ccatcatact agctgtcatg accattttaa ccctcaaaat cagataatcg gtatgaaggt
     4561 cgtagtcata ggagctaaag aaagtggagt tggagcagca ttgctgtgca agcagatggg
     4621 atatgatgtc ctggtcagtg acaatggacc agttatagag ccttttcgaa tggagttaga
     4681 gagatcaaag attgcttttg aggaaaatgg acattcatcc g
//
LOCUS       DAOQMC010000178         4670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8699_length_4670_cov_5.406934, whole genome shotgun sequence.
ACCESSION   DAOQMC010000178 DAOQMC010000000
VERSION     DAOQMC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8699_length_4670_cov_5.406934"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PKV31_07570"
     CDS             complement(<1..1459)
                     /locus_tag="PKV31_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNB92754.1"
                     /translation="MEKLNIAGELSADNIRMLISGMQRRLEKIYKGGGEKKIEKIKAE
                     NRLPARERVSMLLDSDRPSIEIGAFAGYEMYPEYGGCPAGGVIIFLGYVHGRICLVVA
                     NDPTVKAGAWFPVTGKKNLRGQEISMENRIPIIYLVDSAGVFLPMQDEIFPDKEHFGR
                     IFRNNARMSSMGIPQIAAIMGSCVAGGAYLPIMSDEALIVDGSGSIFLAGPYLVKAAI
                     GEDVDKETLGGAVTTTEISGITDYNMPDDKTCIETIRKLVQKFGLPKFTGFDRIKPAL
                     PKKDPNQIVEILPDDRAKPYKTIEIIERLVDDSELIQYKENYGKTILCAYARIDGWAV
                     GIVANNREVIKTKKGEMQFGGVIYSDSADKAARFIMNCNQKKIPLVFLHDVTGFMVGT
                     RSEHGGIIKDGAKMVNAVSNSTVPKFSVVIGNSYGAGNYAMCGKAYDPRLIVAWPTAK
                     IAVMGGAQAAKVLLQIEMSALKGKGENIDPQREQEY"
     gene            1701..2327
                     /locus_tag="PKV31_07575"
     CDS             1701..2327
                     /locus_tag="PKV31_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB92755.1"
                     /translation="MYKPYIVGITGGSGSGKTTFLKSLSEGYSAEEVCILSMDNYYKP
                     RTQQVYDENGVQNFDLVESIDTQAFFNDIIRLVSGETIVQEEYTFNNSLADVEMITIK
                     PAPVMILEGLFIMHMKDLRSLLDLKIYIEAKDALKIIRRIKRDQSERNYPLEDVLYRY
                     ESHVLPSYEKYIFPYKNDVDIIINNNGNTDAVLNILRNHFDVIINSNQ"
     gene            2346..3482
                     /locus_tag="PKV31_07580"
     CDS             2346..3482
                     /locus_tag="PKV31_07580"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNB92756.1"
                     /translation="MKKIGILGGGQLGRMLQAEAQQRNMPVYCMDKSENAPASLYRDF
                     YTIGDIRNYDDVMSFGADKDVITVEIEHINLEALRDLEKAGKEVYPNPDALEIIKNKN
                     IQKSFYHSIGIPIPEFSSFNSKDEAVIVFEQWKNRLPCIYKSAEMGYDGKGVRTIRAI
                     DDVLALDDIPGAFEQRIDIDIEIAVMIARSPDGNYVLYPPLAMYFDPENHILSEVYYP
                     ANMGKNIVEEMNRIAVKVTETLNICGLCAFEFFITSSGEVVLNEIAPRPHNSMHISMN
                     NAHCSQFEQHLRAISGLPLGSSVMEKAGIMYNIIGEEDDYGTAEWTGWHEVMNLHEVY
                     VHLYGKTEIKPARKMGHINIVGDDLSKLKEKLLTIRQYFKNRKS"
     gene            3479..3964
                     /gene="purE"
                     /locus_tag="PKV31_07585"
     CDS             3479..3964
                     /gene="purE"
                     /locus_tag="PKV31_07585"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNB92757.1"
                     /translation="MNEVTPLASIIMGSDSDWPVMSDAAKILEELGIPHEKKVVSAHR
                     TPKMMFEFAENAYSRGIRVIIAGAGGAAHLPGMVASLSCLPVIGVPIKSSNSIDGWDS
                     ILSILQMPGGIPVATVALNGAKNAGILAAQILGTSNDEIYDRIAKYKAELEANVLAKK
                     L"
     gene            4011..>4670
                     /locus_tag="PKV31_07590"
     CDS             4011..>4670
                     /locus_tag="PKV31_07590"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNB92758.1"
                     /translation="MRFCLLLIISFFGWMSSVDAQEEFPVFISGQENYTSYRIPAIIS
                     MPDGRLLAFAEGRVHHTGDFGDVNIVLKISRDRGKTWDNLRTIVDYDELHAGNPAPVI
                     DMADPNYPNGRIFLFYNTGNNTEYDIRKGNGYREVWYITSEDGGETWTQAVNITTQVH
                     RPQQPEVNPDYDFQENWRAYANTPGHALQFSWGKYRGRIYVPAYHSSGDLQFDARDYH
                     AH"
BASE COUNT         1412 a          969 c          962 g         1327 t
ORIGIN      
        1 aatattcctg ttccctctga ggatcgatgt tttctccttt acctttcaat gctgacattt
       61 caatctgtaa aagcactttt gccgcctgtg cgccacccat tacagctatc ttggcggtcg
      121 gccaggcaac aataagcctt gggtcataag ccttgccaca catggcataa tttccggcac
      181 cgtaactgtt gccgattaca acggaaaact ttggtacagt tgagtttgaa accgcattaa
      241 ccattttggc cccgtctttg atgatacctc catgttctga tcgtgtgccc accatgaagc
      301 cggtgacatc atgtaaaaac accaaaggga ttttcttttg gttacaattc atgataaatc
      361 ttgccgcctt atctgctgaa tcactgtata tgacgccccc aaactgcatt tctccttttt
      421 tagtcttaat tacctctcga ttattggcta caatgccaac agcccagcca tcaatcctgg
      481 cataggcaca aaggatagtc tttccgtaat tttccttata ctggatcaat tcagaatcgt
      541 caactaaccg ctcaatgatt tcaatagtct tgtatggttt tgcccgatcg tccggaagaa
      601 tctcaacgat ttggttggga tcctttttgg gcaaggccgg tttgatcctg tcgaaaccag
      661 taaatttcgg caggccgaat ttctggacta attttcgaat agtttcaata catgtcttgt
      721 catccggcat gttataatct gtgataccgc tgatttctgt ggtagtgact gcccccccaa
      781 gggtttcctt atccacgtcc tcgcctatag ctgctttgac gaggtagggt cctgccagaa
      841 aaattgaacc tgatccatcg acaatcaatg cttcatccga catgattgga agataagcac
      901 ctccggccac gcagctgccc ataatggccg ctatttgggg tatacccatg gatgacatac
      961 gggcattgtt gcgaaatatc ctaccaaaat gctctttgtc cggaaaaatc tcatcttgca
     1021 taggcaggaa aacacctgcg ctgtcaacta agtatatgat aggaattctg ttctccatac
     1081 tgatctcctg accccgaaga tttttctttc ccgtgactgg aaaccaagct ccggccttca
     1141 cagtaggatc attggctact acaaggcaaa ttctcccatg tacataacct aaaaagatta
     1201 tgactccacc ggctgggcat ccgccatatt caggatacat ctcataaccg gcgaaggcgc
     1261 caatttcaat actgggtcgg tcactatcaa gaagcattga tacgcgctcc cgtgctggca
     1321 agcgattttc ggcctttatc ttttcaattt ttttctcacc ccctcccttg tatattttct
     1381 ccaaacgcct ctgcatacca ctgatcagca tcctgatatt atcggcactt aattctccgg
     1441 caatatttaa tttttccatc taaaaaataa taatttaaag tattatgagc aaatacgaat
     1501 aatagtttat tatccatcat caaattactc aaatttatat catatataga atgaatcatc
     1561 cgacaaaatt gtcgtacctc tttattcgac atgattgatg aagcgctaaa atataattta
     1621 ttttatttac ctcttaaata attcctattt tcttggagtg aaagcctatt tttgcgcata
     1681 aggataaatt gacaaatttg atgtacaagc catatattgt cggaatcacc ggaggtagtg
     1741 ggtctggaaa gacaacattt cttaagagcc tttctgaagg atatagtgca gaagaagtct
     1801 gcatattatc aatggataac tattacaaac caaggactca acaagtatat gatgaaaacg
     1861 gagttcagaa ttttgatttg gttgaatcca ttgatacaca ggcattcttt aacgatatca
     1921 tccgcctggt gagtggagaa accattgttc aggaggaata cacattcaat aacagtctgg
     1981 cagatgtgga gatgattact atcaagccgg caccggtcat gattcttgaa ggccttttta
     2041 tcatgcatat gaaggacctg agatcattgc ttgatttaaa gatatacatt gaagctaagg
     2101 atgctttgaa aatcatcaga agaatcaaac gcgaccagtc tgagcgaaat tatccattgg
     2161 aagatgtact atatcgttat gaatcccatg tcttaccttc ctacgaaaaa tatatattcc
     2221 cgtacaaaaa tgatgtggac atcatcatca ataataatgg caatacagat gccgtcctaa
     2281 atatattgag aaatcatttt gatgttatca ttaatagtaa ccaataaatt gcatatcccg
     2341 ctctgatgaa aaaaataggt atccttggtg gcggtcagtt agggagaatg cttcaggctg
     2401 aagcgcagca acgaaatatg ccggtatatt gcatggataa gtcggaaaat gctcctgcat
     2461 cattatatag agacttctat actataggtg acatccgcaa ttatgatgat gttatgtcat
     2521 ttggggcgga taaagatgtg attacggtcg agatcgaaca tatcaacctt gaagcattga
     2581 gggatttgga gaaagccggc aaggaggtct acccaaatcc ggatgcactg gagataataa
     2641 aaaataaaaa catacaaaaa tccttttatc actcaattgg cattcctata ccagaatttt
     2701 ccagctttaa tagcaaggat gaagccgtta ttgtatttga acaatggaag aatcgcttac
     2761 cttgtatata caaatcagcc gaaatgggtt atgatggaaa aggagtacgc accattagag
     2821 caattgacga tgtcctggcc cttgatgata taccgggagc tttcgaacaa cgcatcgaca
     2881 ttgatattga aattgcggta atgattgcac gttctcccga tggaaattat gtactctatc
     2941 cgcctctagc catgtatttt gatcctgaaa atcacattct atccgaagtt tattatcctg
     3001 caaacatggg gaaaaatatc gtggaagaaa tgaacaggat tgcagtcaaa gtaacagaaa
     3061 cattgaatat atgtgggtta tgtgctttcg aattttttat tacctcaagc ggtgaagtgg
     3121 ttttaaatga gattgcgccc agacctcata acagcatgca catctcgatg aacaatgcac
     3181 attgcagtca attcgagcag catttgagag caatctccgg attacccctc ggaagtagcg
     3241 taatggaaaa ggcaggtatc atgtacaata ttataggtga agaggatgat tacggaactg
     3301 ccgaatggac cgggtggcat gaagtcatga acctgcatga agtgtacgtt catttatacg
     3361 ggaaaacaga gataaaacct gcccgcaaaa tgggacacat caatattgtc ggagatgact
     3421 tgagtaaact gaaagaaaaa ctattgacaa tcaggcaata tttcaaaaat agaaaatcat
     3481 gaatgaggtt acgcctttag caagtataat tatgggctca gacagtgact ggccggtgat
     3541 gtcggatgct gcaaaaatcc tggaagaact tggtataccc cacgaaaaga aggttgtttc
     3601 tgcccatcgg acacctaaaa tgatgtttga attcgctgaa aatgcttatt cacgaggaat
     3661 tcgtgttata attgccggtg ccggaggtgc agcacacctt ccgggcatgg tagcaagctt
     3721 gagttgcctt ccggtaatag gtgtgcctat taagtcttcc aactcaatcg acggttggga
     3781 ttccattctt tctatacttc agatgccggg aggaataccg gtggctactg ttgcacttaa
     3841 tggagctaag aatgcaggga ttctggcagc tcagatactg ggtacatcca atgatgaaat
     3901 ctatgacaga atagcaaaat ataaagcaga acttgaggca aatgttcttg ccaaaaaact
     3961 ttgattataa tagtcgataa aactgtataa tcgatgatat acaattagat atgagatttt
     4021 gcctcctttt aattatcagc tttttcggtt ggatgtcatc tgtggatgct caggaagaat
     4081 ttccggtttt tatatccgga caagaaaact atacaagtta taggattccg gccataatct
     4141 caatgccaga tggccgatta ctggcttttg cggaaggtag ggtacaccat acaggcgact
     4201 tcggtgatgt caacatcgtt cttaaaatca gtagggacag aggaaagaca tgggacaatc
     4261 ttaggacaat cgtcgattat gatgaattac atgcagggaa tcctgcacct gtgatagata
     4321 tggctgatcc gaattatccc aatggcagga tttttctatt ttacaatacc ggaaacaata
     4381 ccgagtacga tatcaggaag ggtaatggtt atcgggaagt atggtatatc acctctgagg
     4441 atggaggaga aacctggaca caagctgtca atataaccac acaagtccat agaccacaac
     4501 aacctgaagt aaatccggac tatgattttc aggaaaactg gcgtgcctat gccaatacac
     4561 cgggtcatgc cctgcaattt tcctggggta aatatcgcgg caggatatat gtacctgcct
     4621 atcattcctc cggcgacctg caattcgatg caagagacta ccacgcacat
//
LOCUS       DAOQMC010000179         4667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8706_length_4667_cov_3.994579, whole genome shotgun sequence.
ACCESSION   DAOQMC010000179 DAOQMC010000000
VERSION     DAOQMC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8706_length_4667_cov_3.994579"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="PKV31_07595"
     CDS             complement(<1..1869)
                     /locus_tag="PKV31_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNB92759.1"
                     /translation="MKRILIPVFLIIITSGLLSSQTNQSEIIFDSPATHFTRSLPLGN
                     GRLGAMIFGDPHHERIVLNEISLWSGGPQNADRDSARYFLPAIRDFLLNGDNASAQEL
                     LRREFVAKGAGSGFGNGANVPFGCYQTAGELRVDYNNIGTVTDYHRVLDLEVAKSKVS
                     FRVNDTRIIQEAFCDFSDDVIRIRWHSSAPAGLNFSLSLFRKENVSSVNYKHGVLSLE
                     GTLPNGENKGMSYGIRLKVIGCDGNFNFSNGKLNISNAGTCSVIIAIRTNYNYAAGGL
                     LPSTIVSKSLKSDLKASTCHDFHRAWIKSSAIYQKYFNACRLQLFPDENQKVNSLTTP
                     QRLEQYANGGSDVGLPVLYFNFGRYLMICSSKPGLLPTNLQGLWAEEYQTPWNGDYHL
                     NINLQMNYWLAETTNLPAQLKPLFQWTSRLVPNGRKTATAYYGAKGWVAHVISNPWLF
                     TSPGEGADWGSTMTGGAWLACQLWEHYLFSKDTLFLRQYYPVIREAAEFLADVMITEK
                     VHGWLVTAPSNSPENTYIMPGGFHGNTCMGPTMDNQIARQLFNAVIEASELLDIDKGL
                     REILADKVNRLPPTRISPTDGGIMEWLDDWPSAEPHHRHVSQLFGLFPGDEITPFDT"
     gene            2087..2257
                     /locus_tag="PKV31_07600"
     CDS             2087..2257
                     /locus_tag="PKV31_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92760.1"
                     /translation="MSNIFKIKGAQRLTMGEVRKSYNISVPLTSILISKIENSECIFL
                     SGKSVLNSKMEA"
     gene            complement(2722..>4667)
                     /locus_tag="PKV31_07605"
     CDS             complement(2722..>4667)
                     /locus_tag="PKV31_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNB92761.1"
                     /translation="FLILKSEKMNKNKILTLLAGFFFVMAFNSCSKFDELNVDPLAAS
                     DDQVQVEYFINNSIVGAQMDPHIAERVYVLYWKRAARFELGGSLQTGGTDDGWSSDYW
                     RYISGWLNSINTGIEVGNKQIANGTNKLYTNNLVQIARIWRVYLLSEMSDGFGPIAID
                     AFKGKNPEYNSVKDVYYYMLAELKDATAKLDLNAPAADVVNSGSIAFGYKYDYQKWQK
                     YGNSMRMRLAMRLSEVDPGKAQSEFEDAVKDGNYIKTADETFSVKEQPGWDALTGVMS
                     REWNLQFLSATLNNLYTNLGGVKSVDQLPAEIQPAVKPEGYIGQKFPDFYTDKTNDPT
                     VGYWLDGLPNTMDPRAYKTFIIPGWFDNSDFCAYPSWDNSAKTTISEFTDDNGDPVKS
                     IDAKFTWNTTTGGAWAAKGSKNHFRGNNGTTPCLALKYRNSKNRRVFFAPWESFFLIA
                     EAAERGWNAGMSGQAAYEAGIAASFEYHGVSQYLDQYLASESYNYAGTSVNWNHTAEP
                     GVSHTMNYVDGFTGDPGTVDILYPVNNVYKGGAVRNDRLTKIITQKYLAQVPWLPLEA
                     WNDKRRLGLPFFENPAVEDVLPNLPDLNSSNFMTSSIKFYAQRLKYPSSLSNSSKDGY
                     NQAVSQLGGPDNVLTPLWWAKQN"
BASE COUNT         1342 a         1071 c          938 g         1316 t
ORIGIN      
        1 tgtatcaaaa ggtgtgattt catcacccgg aaacagacca aacagctggg atacatgtcg
       61 atgatgcggc tcagctgaag gccagtcatc aagccattcc ataatcccac cgtcagtcgg
      121 actaatccgc gtaggaggaa gcctgtttac tttatccgct aagatttctc gcaacccttt
      181 gtctatgtcc aataattcag atgcctcaat tacagcatta aacaattgtc tggcgatctg
      241 attgtccatc gtgggcccca tacaggtatt gccgtgaaag ccaccgggca tgatgtaggt
      301 attttccggt gaattggaag gtgcagttac caaccatcca tgtacctttt ccgtgatcat
      361 gacatcggcc aaaaattctg cagcctctcg tataacagga taatattggc gcaaaaacaa
      421 cgtgtctttg ctaaacaagt aatgctccca tagttgacaa gccagccagg cgccacctgt
      481 catggtcgaa ccccagtcag caccttcgcc cggagaggta aacaaccatg gattgctgat
      541 gacatgagcc acccaacctt tggcaccgta ataggcggtc gctgtttttc tcccattggg
      601 aaccaggcgg cttgtccatt gaaataatgg tttgagttgt gcaggtagat tagttgtctc
      661 tgccaaccag taattcatct gcagattgat attgagatga taatcaccat tccatggggt
      721 ttggtattct tccgcccata gtccctgaag attggtgggc aataatcccg gcttcgaaga
      781 acaaatcatc aggtaccgtc cgaaattgaa ataaagtacc ggcagaccga cgtcggatcc
      841 tccattggca tactgttcca atctttgtgg agtggtcagg ctattcactt tttgattttc
      901 atccggaaac agttgtagcc tgcacgcgtt gaaatatttc tgataaatag cagatgactt
      961 gatccatgcg cgatgaaagt catggcaggt cgaagcttta aggtcacttt tcagggattt
     1021 cgaaacaata gtggacggca acagccctcc tgcagcataa ttgtaatttg tccttattgc
     1081 aatgatgacg gagcatgtgc cggcattgga aatattcaat tttccattag aaaagttgaa
     1141 atttccatca catccgatta ctttgagccg gatgccatac gacatacctt tattttcacc
     1201 attgggaagc gtgccttcca gagaaagtac gccatgcttg taatttaccg atgagacatt
     1261 ttcctttcta aataaagaaa gactaaaatt caaccctgcc ggtgcagaag aatgccaccg
     1321 gatacgaatg acatcatccg aaaaatcaca aaatgcttct tgtatgattc ttgtgtcatt
     1381 gaccctgaag gaaactttac tttttgcgac ctcaagatca agtaccctat ggtagtctgt
     1441 taccgttccg atgttattat agtccactct caattcacct gcagtctggt aacaaccgaa
     1501 gggtacattt gcaccattgc cgaaaccaga acccgctccc tttgccacaa actcccttct
     1561 gagcaattcc tgagcgctag cattatcgcc attaagtaag aaatcccgaa tagccgggag
     1621 gaagtacctt gcactgtcgc ggtcagcatt ctgaggcccg ccggaccata aggagatttc
     1681 attgagtacg atgcgctcat gatgagggtc tccgaaaatc atggcaccaa gtcgaccatt
     1741 accaagaggg agagaccggg taaaatgagt agccggacta tcgaaaatga tttctgactg
     1801 attagtttgg gaagacagca atcctgaagt aataataatc aaaaaaacag gaatcagtat
     1861 tctcttcacc ttattgaaat ttattgagtc cggacaaatt tacagtctat catttacttg
     1921 ccacaaaata ataatttatc cgctcaaaaa atgtatatta tatcattcca acgccagtgg
     1981 cactcaattg cccataaaat ttgtccaaag tatagatgtg tacgacttac ttactatagt
     2041 acctgaaaat ctcttcaaag ctggcacaat aatgatggat cttcctatgt ctaacatttt
     2101 taaaatcaaa ggtgctcaac gactcactat gggcgaggtt agaaaaagtt acaacatttc
     2161 tgtgcctttg acttcgatcc tgataagcaa gatagaaaac tctgaatgca ttttcttatc
     2221 cggcaaatct gttttgaact ccaagatgga agcctaatca tacgttcttt gccaacttcc
     2281 ccttttatga aaccattaca ggccaaagag aatgtcacct cattgaatgc agatgtggcg
     2341 agaccattaa agaaaaaaag ctgacaatta ttatattata ttttttacaa tactttatga
     2401 attgataatt ttatatttgc aatatatact caatcatata atgaattcct taggatactg
     2461 acttaactgt cttaacttga atcttacctt tgaggcagcc gacttgaata tagttacttt
     2521 aaggcgacta tcgtctcagc agcttcacta ccccggtcta atcataagta tcaaacaata
     2581 aatgcagaaa tgcatcttcc tccttttgat ttgattgatt atcaacatta catgatagcc
     2641 agaaataaaa aaggcggaca tcgctgcccg cccaaaaaat attcagtaca cttcaattat
     2701 tcaaaaaact cgcgtccttc attaattttg ttttgcccac cataatgggg ttaatacgtt
     2761 gtcaggtcca cctaactggc tcactgcctg gttatatcca tccttactgc tattagagag
     2821 ggaggatggg tacttaagcc tttgagcata aaacttgatg ctactggtca taaagttgga
     2881 gctgttcaag tcaggcaaat taggcagaac gtcttcaaca gccggatttt caaagaatgg
     2941 aagtccaagt cttcgtttgt cattccatgc ttctaacggc aaccaaggta cctgtgccag
     3001 atatttctgg gtgatgatct tggtgaggcg gtcattgcgt acggcaccac ccttgtaaac
     3061 attattcaca gggtaaagga tatcaactgt tccggggtct cctgtaaagc catccacata
     3121 attcatggtg tgcgaaactc ccggctcggc agtatgattc caattaactg aggtacccgc
     3181 atagttataa gattcggaag caaggtattg atcaaggtac tgagaaacac catgatattc
     3241 aaaacttgct gcgattcctg cctcataggc agcttgtccg ctcatgcctg cattccagcc
     3301 gcgctctgca gcttcggcaa taaggaagaa agactcccat ggcgcaaaga aaaccctgcg
     3361 attcttactg tttctgtact tcagggccag acaaggggta gttccgttgt tgccgcggaa
     3421 atgattcttt gagcctttgg cagcccatgc tccgccagtc gttgtgttcc aggtaaactt
     3481 ggcatcaatg gacttaaccg gatctccatt atcatcagta aattctgaaa tggtagtttt
     3541 agcagaatta tcccaggaag gatatgcgca aaaatcagaa ttgtcaaacc aacccggaat
     3601 tataaaggtt ttatacgctc ttggatccat tgtattaggc aaaccatcca gccagtaacc
     3661 tactgtcgga tcattggtct tatccgtata gaaatcgggg aatttctggc cgatatatcc
     3721 ttccggctta actgccggct ggatttcagc aggaagttga tccacagatt ttaccccacc
     3781 aagattggta tatagattat tcaaagtagc agaaaggaat tgaaggttcc attcccttga
     3841 catcacaccg gtgagtgcgt cccatcccgg ctgttctttt acgctaaaag tttcatctgc
     3901 agtcttgatg taattaccat ctttgacggc atcttcaaat tctgactgcg ccttacccgg
     3961 atccacttcc gaaagtctca ttgccaaacg cattctcatg gaatttccat atttctgcca
     4021 tttctgatag tcatacttat agccgaatgc aatgctgccg gaattgacta catcagcagc
     4081 tggagcgtta agatccagct tggcagtagc atccttcaac tcagcgagca tataataata
     4141 tacatctttc acgctgttgt attcagggtt tttacctttg aaagcgtcta tagcaatagg
     4201 tccgaatcca tcagacatct cagaaagcag atagactctc cagatacggg ctatctgtac
     4261 caggttgttg gtatatagct tattagtacc attggcaatc tgcttgttgc ccacttcgat
     4321 tccggtattg attgaattca accaaccgga gatgtatcgc caatagtcgg aagaccaacc
     4381 atcatcggta ccaccggtct gcaaagaacc acccagttcg aaacgagcag cacgtttcca
     4441 gtatagaaca tagacccgct ctgcgatatg tgggtccatc tgggcgccta caatcgagtt
     4501 gttgataaag tactccacct gaacctgatc atcactcgct gctagcgggt caacattcag
     4561 ctcatcaaat ttggagcacg aattgaaggc catgacaaag aaaaatccgg ccaacaaggt
     4621 taatatttta tttttattca ttttttctga ttttaaaatt agaaatt
//
LOCUS       DAOQMC010000180         4657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8731_length_4657_cov_4.456758, whole genome shotgun sequence.
ACCESSION   DAOQMC010000180 DAOQMC010000000
VERSION     DAOQMC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8731_length_4657_cov_4.456758"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKV31_07610"
     CDS             <1..835
                     /locus_tag="PKV31_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNB92762.1"
                     /translation="GGIAEMSELPKEVRTKTDILDSVGNTTAAIGKGFAIASAALTAL
                     GLFAAYVTFTGIDGINIFKADVLAALFIGAMIPVVFSALAMSSVGKAAMDMVKEVRRQ
                     FKEIPGIMEGKATPEYGKCVEISTKAALREMMLPGALTIVVPILVAIFMGAEALGSYM
                     AGVTVSGVLWAIFQNNAGGAWDNAKKSFEAGVEIDGVMTYKGSDAHKAAVTGDTVGDP
                     FKDTSGPSMNILIKLTCLIGLALAPILGGHNAKHDTGLKSTPKIEKMYKQEAPTNVIK
                     F"
     gene            1124..2908
                     /gene="sppA"
                     /locus_tag="PKV31_07615"
     CDS             1124..2908
                     /gene="sppA"
                     /locus_tag="PKV31_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNB92763.1"
                     /translation="MKSFLKIVLGSCVGVIFAMALFSFLSVIAFSSMSSSLTGGGGKE
                     ISPNSVLRISLEQPMPDKTNNIESSPLSFANRTFVGIVDAVKLIKHAKTDENIKGIYI
                     NPGPGMAIGSAALKDLHDALLDFKTSKKFVIAYGEALSQGGYYLSSTADDIYLNPVGE
                     VYFKGLGMSIMYFKNLMDKLKIKMNVFYVGKFKSATEPFRSDHMSPENRLQLKTFLNG
                     MFSQMVEDIARNRKLDPAAVKKVADELSIQAPEDALALNFIDKLAYEDEVFEEIKTKL
                     KLKAKDKINFVSLNEYYDGTTLEKDYNAKEKIVLIYAEGEINDSDSHDAVIGGKSYVR
                     YLRKARLDDKVKAVVLRVDSPGGSAYASEQMWHEIDLLKKAGKKVIVSMGNLAASGGY
                     YISCAADRIFANNNTITGSIGVFGMIPNVSGFYNDKLYLTMDSVKTGPNGLGINGYYD
                     MTPAETSLIQRSIESIYKTFTKRVADGRKLSIETVDSLAQGRIYSGKDAVKIGLVDEI
                     GDVEDAIASAASMAKLKEYRLVEYPETKPPFEKFMEKLSGKETDDNAVKAAVTRELKA
                     IAPEYIEVKRLLQNNRIQARLPMTITIN"
     gene            2932..3636
                     /gene="trmD"
                     /locus_tag="PKV31_07620"
     CDS             2932..3636
                     /gene="trmD"
                     /locus_tag="PKV31_07620"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNB92764.1"
                     /translation="MKIDIIAVIPDLMKSFFENSIIKNARTKGHLTINVIDLRNYGLG
                     NYKQIDDYQYGGQAGMVLMPEPLSNCIEMLQASTLYDEIIYLTPDGTTLTQKTSNKLS
                     LKENILIICGHYKGIDQRIRDKYVTMEISIGDYVLSGGETAAIVLVDTIGRLIPGVIN
                     DETSALSDSFQDDLLAPPVYTRPAEFQGMKVPDILLSGHEKNIREWQEQQAFEKTKRL
                     RPDLFRKWEEESDLPV"
     gene            complement(3619..>4657)
                     /locus_tag="PKV31_07625"
     CDS             complement(3619..>4657)
                     /locus_tag="PKV31_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB92765.1"
                     /translation="KRFGIELTAGVVYTGTEIEAALYGDTSYTSLNMAGYVSMDKVFF
                     KRLTFSAGARYEYNALRSPEVVEGFVIPGGKTSESKPVFKFGLNYLLNPFASVRASFG
                     QGYRYPTVAEKFIKTTVGAIRVAPNPNLQSETGYTAEIGIKHGLKISSWKGFIDAAFF
                     RSDYDNMMEFTLRNVSKGFQSYNIGDTRIQGFELGIQGAGDIGDVTVTTYAGYTYVDP
                     KYRNFNEIDTTKSTVSYNILKYRMKHAVRADLGLVYHGIEIGGYFQYNSKMEAIDRIF
                     NFGIPGVRQFRESHDFGSRVFGFRVVVPVIKDQLRLGINLNNAFNQEYSIRPGLLEAP
                     RNISARLDWKI"
BASE COUNT         1455 a          952 c          973 g         1277 t
ORIGIN      
        1 gggaggtatt gctgaaatga gtgaactgcc aaaagaagta agaacaaaaa cggatattct
       61 tgattccgtt ggtaatacaa ctgcagctat cggaaaagga tttgctatcg catcagccgc
      121 tttgactgcg cttggccttt ttgcagccta tgtcaccttc accggtattg atgggattaa
      181 tatctttaag gctgatgtgc ttgctgctct atttatcgga gccatgatac ctgtcgtgtt
      241 ctcagctctt gccatgagct cggtaggtaa agcagccatg gatatggtga aggaggtaag
      301 aagacaattc aaggagatcc ccggcataat ggaaggaaaa gcgacacctg aatacggcaa
      361 atgtgttgaa atcagtacaa aagcagctct cagggaaatg atgttgcccg gtgcacttac
      421 catcgttgtc ccaatactgg tggccatctt tatgggtgct gaagccttgg gttcgtatat
      481 ggcaggtgtg actgtttcag gtgttctttg ggctatcttc caaaacaatg caggaggggc
      541 atgggacaat gccaaaaaat ccttcgaagc tggagtagaa atagatggtg taatgaccta
      601 caaaggctca gatgcccata aggctgctgt gacaggtgac acagttggtg atccatttaa
      661 agatacatca ggaccttcga tgaatatcct gatcaaattg acttgtctga ttggcctggc
      721 attagcacca atactgggcg gacataatgc aaagcacgat acaggcctga aatctactcc
      781 gaagatcgag aagatgtaca aacaagaggc cccaaccaac gttataaagt tttaacaaac
      841 agataattct attgataaaa ggctgtaaaa tacttacagc ctttattttt tttatttggt
      901 cttgaaaaaa gtaccttata tccaaaaatt taaactaata agtacttttt ggtgtatcat
      961 tgtataaagt gaactaaaaa tatgtattca actggttttt gatcatttta agattccaaa
     1021 ctcaaataca gtatgatact taacacatat tatttcacca ctttatacat aaaacaacag
     1081 ccgtaaaaga actaaaaata tataataatt aagtttctct ttcatgaagt cattcttaaa
     1141 aatcgtctta ggatcctgcg taggtgtcat ttttgccatg gcactatttt cttttctctc
     1201 tgttattgct ttttcttcca tgtcttcgtc tttaaccgga ggaggcggaa aggaaatttc
     1261 ccccaacagt gttttgcgca ttagtttgga gcaacccatg cctgacaaga caaacaatat
     1321 cgaaagtagc ccactttcct ttgccaatcg cacttttgta ggtattgtcg atgctgtaaa
     1381 gttaatcaaa catgccaaga ctgatgagaa tatcaaaggg atctacatca atcctggacc
     1441 aggcatggcc attggatctg cagcacttaa agacctgcat gatgcgcttt tggattttaa
     1501 gacgagtaag aaattcgtta ttgcctacgg agaagcactt tcacaaggtg gatactacct
     1561 tagttccaca gcggatgaca tctatcttaa tcctgttggt gaagtatatt ttaaaggctt
     1621 ggggatgagc ataatgtatt ttaaaaacct gatggataag ctaaagatta agatgaatgt
     1681 cttttatgtc ggcaaattca aatccgccac tgagccgttc agatccgatc atatgagccc
     1741 ggaaaacaga cttcagttga agacatttct caatggtatg tttagccaaa tggttgagga
     1801 tatagccagg aacaggaaac ttgacccggc agcagttaaa aaagtagctg acgaattatc
     1861 aattcaggcc cccgaagatg ctttagctct gaatttcatt gacaaactgg cttatgaaga
     1921 tgaagttttc gaagaaataa aaacaaagct aaaattaaaa gccaaggata aaatcaattt
     1981 tgtttcctta aatgaatatt atgacgggac aaccttggaa aaggactata atgcaaaaga
     2041 aaaaatcgtc ctgatatatg cagaaggtga aataaatgat tctgacagcc atgatgctgt
     2101 cattggcggc aaatcatatg tgcgttatct cagaaaagca agactggacg ataaggtgaa
     2161 ggcagtagtc ctaagagtgg acagcccggg gggtagtgca tatgcctcag agcaaatgtg
     2221 gcatgaaatc gacctgctta aaaaagcagg taaaaaagta atcgtgtcca tgggcaatct
     2281 ggcagccagc ggcggatatt acatttcatg cgcagcagac agaatatttg ccaataataa
     2341 tacaattacc gggtcaatcg gtgtttttgg catgatacca aatgtgtccg gattttataa
     2401 cgacaagcta tacctcacta tggattctgt gaagaccgga ccaaatggat tgggtatcaa
     2461 tggttattat gacatgacac ctgcagaaac ctccttgatt caacggtcga tcgaatcaat
     2521 ctacaaaacc tttacaaaga gagtagctga tggaagaaaa ttatcgattg agacggttga
     2581 cagtcttgct cagggccgaa tctattcagg caaggatgct gttaagattg gcctggtcga
     2641 tgaaataggt gacgtggagg atgctattgc cagtgctgcc tctatggcca agctaaaaga
     2701 atatcgtctg gttgaatatc ctgagacaaa gcctcctttc gaaaaattca tggaaaaact
     2761 ttcaggaaag gaaactgacg acaatgcggt taaagctgca gttacaaggg aacttaaagc
     2821 cattgctccc gaatatattg aggttaaacg cttgcttcaa aacaatcgca tacaggcccg
     2881 gctccccatg acaattacta tcaattgaca atctgaagcc ctgtattgcc tatgaagatc
     2941 gatatcattg cagtcattcc ggacctgatg aagtcctttt ttgagaattc catcatcaaa
     3001 aatgccagga caaaaggcca tttgacaatc aatgtcattg atttacggaa ttatggtcta
     3061 gggaattaca agcagattga tgattatcag tatggcggtc aggcaggaat ggtactgatg
     3121 cccgaaccat tgagcaattg tattgaaatg ctacaagcct caacccttta tgatgaaata
     3181 atctatctga ctccggatgg aactacactc actcaaaaga catcaaacaa gctgtcactc
     3241 aaggaaaaca tcctaataat ctgtggccat tacaagggga ttgaccaacg aatacgagac
     3301 aaatatgtca ccatggaaat atcaattggt gactatgtct tgagtggagg ggagacagca
     3361 gccatagtcc tggtagatac tataggaagg ttgattcccg gagtgatcaa cgatgaaaca
     3421 tccgcattgt ccgatagttt ccaggatgat ttactggcac caccggttta taccagaccg
     3481 gcagaatttc agggaatgaa agtacccgat atcttacttt cgggccatga aaagaatatc
     3541 cgcgaatggc aggaacaaca agcattcgaa aaaactaaac ggctgcgacc tgacctgttc
     3601 agaaaatggg aagaagaatc agatcttcca gtctaacctg gcactgatat ttctcggagc
     3661 ttcaagcaat ccgggtcgga tgctgtattc ctgattgaat gcattattca gattgatgcc
     3721 caatcggagt tgatctttga tgacaggtac cactaccctg aatccgaata cacgggaacc
     3781 aaaatcatga ctttccctga attgccttac cccgggtatc ccaaaattaa atattctgtc
     3841 tatagcctcc atcttgctgt tgtactgaaa gtatccacct atttcgatcc catgatatac
     3901 aaggcccagg tccgccctga ctgcatgttt catcctgtat ttcagtatat tataacttac
     3961 cgtggattta gtcgtgtcga tttcattaaa atttctatat ttgggatcta catatgtata
     4021 accggcatat gttgtaactg taacatcccc tatatctccc gcaccctgaa tgcccagttc
     4081 aaacccttga atacgggtat ctccgatgtt gtacgattgg aatcctttgg acacatttct
     4141 tagggtaaat tccatcatat tgtcataatc agacctgaaa aaagccgcat caataaatcc
     4201 cttccatgaa gaaatcttaa gaccgtgctt gatacctatc tcagcggtat aaccagtctc
     4261 agattgtaag ttcggattag gagcaacccg aattgcacct accgttgtct tgataaattt
     4321 ttcagcaact gttggataac ggtaaccttg cccgaaagaa gcccttaccg aagcaaatgg
     4381 atttaagaga taattcaggc caaatttaaa gacaggctta ctctctgaag ttttaccccc
     4441 cggaatgaca aacccctcta ccacttccgg actcctcagt gcattatatt catatctggc
     4501 accggcactg aaagtaagcc ttttaaagaa aaccttgtcc atagaaacgt aacccgccat
     4561 attcaaagat gtataggaag tgtctccata taatgctgct tcgatctcag ttcctgtata
     4621 gaccacaccg gctgtaagtt ctataccgaa tcttttc
//
LOCUS       DAOQMC010000181         4645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8768_length_4645_cov_4.521786, whole genome shotgun sequence.
ACCESSION   DAOQMC010000181 DAOQMC010000000
VERSION     DAOQMC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8768_length_4645_cov_4.521786"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKV31_07630"
     CDS             <1..286
                     /locus_tag="PKV31_07630"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNB92766.1"
                     /translation="ALTFLEEKIDAMELELPPLKHFILPSGHTSTSFAHMARCVCRRA
                     ERRIVSLSHEEEIGEEPLAFFNRLSDYFFVLARYLTHLAGKKDVEWNPRD"
     gene            340..2430
                     /locus_tag="PKV31_07635"
     CDS             340..2430
                     /locus_tag="PKV31_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNB92767.1"
                     /translation="MEQKINFSILRNLTASYRILIILTSALIISLLLPRVKTVNYEFE
                     EGSKWKYSDLRAPYDFAIRKTENEIAAEKEAIVKNFIPYYKKNESTTENLNDRILEEY
                     NTYQSHAGGDTALFLSNYKDINIKQIRSVFQKTYRTGILEANDIYKDREKEFLIHIVE
                     GNIISLKPRAAIPTVVQAKNELHRDIRQAGEHGPLLYPVIDALIEPNIFLDQTLSNEF
                     LNQAFKNIATNEGIVRKGDIIINRNSTISKDTYQKLISLRELDDAGNTGKLSYFKSFL
                     AYFVFISVILMFYVLYVRTYVREVFLIFKKMLFMHLWLILYVFMTYFVIKSSSLDLLM
                     IPFCIAPIVIKNFYNQRLAFFTLVAILTIVALFGPVTKDFIILQITAGIMVVIVTKET
                     RYWSKFFLLLLFLLGTYIFTYLFLTYIKNNNLADFNLNSIWWICLNVFLTLLAYPLIP
                     LLERLFGFTSGITLAELSDLNNNLLKELSIKAPGTFQHVLHVATLAEAAAEKIGANSS
                     MARVGALYHDIGKMKAPYNFTENQTNINPHDSIDHVTSAKLIIEHVPEGVKIAKKNGL
                     PNVIIDFIKTHHGTTKVEYFYRKFVKEHADHAVDEKVFTYPGPRPRTKEQVVVMMADT
                     LEAAVKSLINPTEEEINDMVDKLIRAKIEDGQFDRSDITFEDVQTCIRIFKNMLVNMS
                     HIRIAYPDPRPETN"
     gene            complement(2400..3707)
                     /locus_tag="PKV31_07640"
     CDS             complement(2400..3707)
                     /locus_tag="PKV31_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB92768.1"
                     /translation="MINTSRTRVLDFVLRNFGSIASLCKWGMNAGVYLTISGLFASRF
                     LIACGMIVMGCASLLNMTTLHKQDIRIRLHLVKPVAIAVSLLVLGVVTSFLGSEQLGF
                     AMVRLRLYVAFLLIPLILAFAPPIDRNHIRNYLLYALSSAAISSIFIQLNYFQHYETI
                     SKELSTGKPIPTPIPHIRYGMFLSFTFLGGLFAFLSKTYPVRWQVFVLLTTLYLGFSI
                     LFLSVRTAWLVSFLGLLIILAYFIMQKRRITLISIVIIAAIGGSILAYLFIPSVKIKA
                     GYTLYDWRKFREDKGSEYSDSERLYSLQNGWSLWKENKWLGVGSGDLHRQLEQNAIKN
                     HYPGSQIPHNQWLMTLVCGGLLSLAFTLLFWYQLWRNPIHRKLFLFHLLVILYLVTFF
                     FEPTFETSIGVLSFVFILIMILLQSYQGEQKSRLQLVSGRGSG"
     gene            complement(3750..>4645)
                     /locus_tag="PKV31_07645"
     CDS             complement(3750..>4645)
                     /locus_tag="PKV31_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HNB92769.1"
                     /translation="VLGLVNKIMEVPCKDNFIPLPVNFLEDIGKDEEVSGVKTYFVSR
                     PDIHVHPIPSKNSLKIEVFDDNESSVMYYTIDGSDPRTRGFEYKSPITIREAGTYTIK
                     AAANKNGVWSPVTQEKTSLRFNITSIELDPEPDAKYFYDGTDELIDGDTAGLSYSEEK
                     WLGFQGTDVDMLFNFGSKREIRSVDLSALHDYYSWIFLPVRVTFYAGNDRNHLVEIGT
                     ISHAVDRNDVTIRKLHYSVIMHDALLKSLGGSIVKKKKGKRSLYVKYLRIKVEAQKVA
                     PEWFSLPGAQVWFFTDEVRIE"
BASE COUNT         1455 a         1028 c          889 g         1273 t
ORIGIN      
        1 ggcgctcaca tttctggagg aaaagattga tgctatggag ctcgaattac caccattaaa
       61 gcatttcatc ctaccgtccg ggcatacatc cacatcattt gcccatatgg ccagatgtgt
      121 gtgcaggagg gctgaacggc ggattgtttc tctcagtcat gaagaggaaa ttggtgaaga
      181 gcctctggca tttttcaaca ggttatccga ttatttcttc gtcctagcaa ggtatttaac
      241 tcatttggcc ggtaaaaagg atgtagaatg gaatcccagg gattgaaata ggggcagaac
      301 aaaccattta tcaataaaaa ctcaggctaa atcctttcta tggagcaaaa aataaatttc
      361 agtatattac gaaacttaac tgcgtcatac aggatactca tcatcctgac ctctgctttg
      421 ataatcagcc tcttgttacc ccgggtgaag actgttaact atgaatttga agagggaagt
      481 aaatggaaat acagtgactt aagggcaccc tatgactttg cgatccgaaa gactgagaat
      541 gaaattgccg ctgaaaagga agccatagtc aaaaacttta taccttatta taagaaaaat
      601 gaatccacaa cggaaaacct gaatgataga atccttgaag aatacaatac ctatcaaagt
      661 catgccggcg gggatacagc tctgtttctc agtaattaca aggatatcaa tatcaaacag
      721 atccgttctg tttttcagaa aacttaccgg accggaatcc tggaagccaa tgatatttat
      781 aaggatcggg aaaaggaatt tttgattcat attgtagagg gaaatatcat ttccctaaaa
      841 cccagggcag ccattccaac agttgttcag gcaaagaatg agcttcatcg ggacatcagg
      901 caagccggtg aacatggccc cctactctac cctgttatcg atgctctcat cgagcccaac
      961 atctttctgg atcaaacctt gtccaatgaa tttctcaacc aggcattcaa gaacatagcc
     1021 accaatgaag gaattgttcg taaaggtgac atcataatca accgcaattc aaccatctca
     1081 aaggatacct atcagaagtt aatttctcta agagaattgg atgatgcagg taacacaggt
     1141 aaattatcgt atttcaagtc attccttgcg tattttgtct ttatttcagt tattctcatg
     1201 ttctatgtgc tttatgtacg gacctatgta cgcgaggttt ttcttatctt caagaagatg
     1261 ctttttatgc atctttggct cattctatat gtctttatga cctatttcgt catcaagtca
     1321 tccagtcttg acctactgat gattcctttc tgcatagcac ccatagtcat taagaatttt
     1381 tataatcagc gacttgcatt ttttacattg gtagccatcc tgaccatcgt tgccttgttc
     1441 ggtccggtaa caaaggattt catcatcttg cagataacag ccggaataat ggttgtgatt
     1501 gtaacaaaag aaaccagata ttggtccaaa tttttccttt tgctgctatt tcttttaggt
     1561 acgtatatct ttacttatct ctttcttacc tatatcaaaa acaacaacct cgcagacttt
     1621 aatttgaaca gtatttggtg gatctgcctg aacgttttcc tcacgttgct tgcctatccg
     1681 ctgatcccgt tactcgagcg attattcgga ttcacatccg gaattacgct tgcagagcta
     1741 tccgacctga acaacaattt gctgaaagag ctttccataa aagcaccggg gactttccag
     1801 catgtattgc atgtagctac acttgcagag gcagcagcag aaaagatcgg agccaatagt
     1861 tctatggcac gggtgggagc attgtatcat gacattggca aaatgaaagc accctataat
     1921 tttaccgaaa atcaaaccaa catcaatcct cacgactcca tagatcatgt caccagtgcc
     1981 aagcttatta tcgaacatgt tccggaaggg gttaaaatcg caaaaaagaa tggtctgccc
     2041 aacgtaatca ttgatttcat caagacccat catggtacga caaaggtgga gtatttctac
     2101 aggaaatttg tcaaggaaca tgccgatcat gctgttgacg agaaggtatt tacctatccc
     2161 ggacccagac cacgtacgaa agaacaggta gttgtcatga tggccgatac gctggaagcc
     2221 gccgtcaaga gtctgatcaa ccctacagaa gaagaaatca atgatatggt ggacaaatta
     2281 attcgtgcaa agattgaaga tgggcaattc gacaggtctg atataacctt cgaagatgta
     2341 cagacctgta tccgcatttt taaaaacatg ctggtcaata tgagtcatat cagaatcgct
     2401 tatcccgatc cacgacctga aaccaattga agtcgcgact tttgctcacc ctgataagat
     2461 tgtaacaata tcatgattaa tataaataca aatgaaagta ctccaataga agtttcaaag
     2521 gttggttcaa agaaaaaagt gactaaataa agaatcacta agagatgaaa taggaataac
     2581 tttcggtgta ttggattcct ccatagttgg taccaaaaca acaaggtgaa agcaagggag
     2641 agcaatcccc cgcacactaa ggtcatcagc cattgattat ggggaatctg acttcccgga
     2701 taatgattct tgatggcatt ctgctccaat tgtcgatgca aatcacccga gcccactccc
     2761 agccatttat tttccttcca cagcgaccac ccgttttgca aagaatatag ccgctcacta
     2821 tcagaatact cacttccctt atcttccctg aacttccgcc aatcatacag ggtataacct
     2881 gcttttatct ttaccgaagg gataaataaa tatgcaagaa tacttcctcc gattgctgca
     2941 atgatgacaa tactgattag cgttattctt ctcttctgca tgataaaata agccaaaatg
     3001 atcagcaagc ctaaaaaact cacaagccaa gcagtacgga ccgaaagaaa cagaatggaa
     3061 aagcccagat acaaggtagt caataaaaca aatacctgcc atcttacagg ataagtttta
     3121 ctcagaaaag caaagaggcc tcccaaaaaa gtaaaactca gaaacatacc atatcggatg
     3181 tgcggaatag gtgtcggtat tggtttacca gttgaaagtt ccttgctgat tgtttcataa
     3241 tgctgaaagt agttcaactg aataaaaata ctggaaatag cagcacttga cagagcatac
     3301 aacagataat ttctgatatg attcctatcg ataggcggtg caaaggccag gataagtgga
     3361 atcaacagga aagccacata caacctcaac cgcaccattg caaaaccaag ctgctcactc
     3421 cctaaaaagg aggtaaccac tcccagcaca agcagagata cggcaattgc caccggcttt
     3481 accaaatgta agcgaatccg gatgtcttgt ttgtgtaatg tagtcatgtt taacaaggat
     3541 gcacatccca tcacaatcat tccacaggca atcagaaatc tactggcaaa caagccggaa
     3601 atggttaggt atacaccggc attcatgccc cacttgcata atgacgcaat acttccaaaa
     3661 ttgcggagga caaaatccag tacccttgtt cttgaagtat ttatcatatt ttgccggaaa
     3721 ctaaagctta tgcctgtaag aaataaagtt tattcaatcc tgacttcgtc cgtgaagaac
     3781 caaacctgag cgcccggcaa agaaaaccat tcaggtgcaa ctttctgggc ttccaccttg
     3841 atccgtaagt atttaacgta caagcttctt ttgcctttct tcttcttcac gatactccct
     3901 cccagtgatt tcaggagtgc gtcatgcatg attaccgagt aatggagttt cctgatagtt
     3961 acatcgttcc tgtctacagc atgagaaata gtcccgatct ctaccagatg atttctgtcg
     4021 ttacctgcat aaaatgtaac tctgacaggc aggaaaatcc aagaataata gtcatgcaag
     4081 gctgacaaat ccacagacct gatctcacgc ttcgaaccaa aattgaagag catatcgaca
     4141 tccgtacctt gaaaacccaa ccatttttcc tcggaataac tgagacctgc agtatcgcca
     4201 tctatcaact catcggtccc atcataaaaa tattttgcat caggttccgg gtctagttct
     4261 atcgaggtaa tattgaacct caatgaagtc ttttcctgcg ttacaggtga ccacacacca
     4321 tttttgtttg ctgcggcctt gatggtatag gtaccagcct ctctaatggt aatcggtgat
     4381 ttgtattcaa atcctctggt gcgtggatcg cttccatcga ttgtataata catcaccgat
     4441 gactcattgt cgtcaaaaac ctcgattttg agagaatttt ttgagggaat aggatgcaca
     4501 tgaatatcag gccggcttac aaagtaggtc ttcaccccgg agacctcttc atctttgcca
     4561 atatcttcca ggaaattgac cggaagtgga atgaaattat ctttacaagg tacctccata
     4621 atcttgttga ctagaccaag aacat
//
LOCUS       DAOQMC010000182         4643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8771_length_4643_cov_5.120532, whole genome shotgun sequence.
ACCESSION   DAOQMC010000182 DAOQMC010000000
VERSION     DAOQMC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8771_length_4643_cov_5.120532"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="PKV31_07650"
     CDS             <1..1976
                     /locus_tag="PKV31_07650"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB92770.1"
                     /translation="IGQSNNVNEQNFAADDMAAMQTTSGGGMRGHGGPGGPPRGGGEF
                     GRNNNPFMTNTSGGIVTTHALGVNYTDKWADKVVFQGSVFGNLTNTNLIQSSFTEYFT
                     GQNSKDNTVSDRDGKSFRFNARVEYTIDSFQSVIYTPSYNYGYNTSNYINNSDIFTGE
                     TPLSKYDNKSSSYGKNVSFNNELMYRKRFSTQGRTISLRLDQSYSNNLPRTDQNLLTI
                     SYDTVNIEILEKQYVESNNYNRSYSAEVNYTEPIAKDHFLMFGYNYSIKDNDIDKRLY
                     DLGNGEQPGEQNLITSLSNVTDNLYQSHRPRLDYRFQNKKLNLSAGVGYQYSILNTDK
                     TFPQVEHVNKTFKNVLPSAMVRLSISKTKNFRLFYRAGTQEPSVSQLQSVIDNSNTLQ
                     LTSGNPTLDQSTTHRMMMFFSSTNQQTGSNFFIGGGGMATKDYIASQVIYSDKDTILA
                     GYGNMPAGARYTYPINLDGYYSIRGFSSFGFPFYLIKSNVSLNVNGSMINAPSKINSQ
                     INHSRSKNVGGGVTISSNFSQNLDFTIATQVGFSDVTNDINTQADNTYFNQNVSGRFN
                     WIFFKNFVFSTDAAYYYNKGLESVSQTNYTIWNAAFGYKFLKKNAAEIRIQAFDILNN
                     NTALTRNVTETYYADVSTNVLNRYFMLQFSYKF"
     gene            2145..2957
                     /locus_tag="PKV31_07655"
     CDS             2145..2957
                     /locus_tag="PKV31_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92771.1"
                     /translation="MQVKDKTVVITGGGNGMGREVVLNMLHKGARVAAVDVNGSALEE
                     TKRLAGANADRLSIHVLDITDKDAVFAFPQIVIAEHGSVDIVINNAGIIQPFVRVNDL
                     PYDKITRVFDVNFFGLLYMTKSFLPHLLERPEAHLVNVSSMGGFLPVPGQSIYGASKA
                     AVKLLTEGLYAELRHTRVHVSIVFPGAIGTEITRNSGVDVPNMAAGVDAKKQAARTTS
                     PDKAAEIIVNGILKNKNRIFVGGDASFLDRLYRLAPTFATNFIAGKMKDLLK"
     gene            3074..3694
                     /locus_tag="PKV31_07660"
     CDS             3074..3694
                     /locus_tag="PKV31_07660"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HNB92772.1"
                     /translation="MPITTDIPEGYERIKKTIDIYRANIYGFLILIPVSAMYLLPYYL
                     VWQKIPAIGSILSDQGHPPIRVLIYTIVALVAGVVLHELIHGITWSFFTRHGFRSIRF
                     GIMSKALTPYCHCKEPLSKNQYILGAIMPSILLGVIPGIWAIAMGNAVALYWGVFFTI
                     AAVGDFMIISLIRKEARNVFILDHPTEAGCYVFRRKTTPEKDESIL"
     gene            complement(3973..4419)
                     /locus_tag="PKV31_07665"
     CDS             complement(3973..4419)
                     /locus_tag="PKV31_07665"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB92773.1"
                     /translation="MKNLMTDKSIFVKSLVLVVCAAIVILLPSAASIKTVNLNGKIKY
                     DQSTVASDAAVSLLNETDLSLVTSVITDREGNFRFAKLKPGKYFVAAGAFGNKLEVFG
                     PYEVSGKDRNITIEELIIKRPSGTTGPIIKAKKDHAPVINRSIRIS"
BASE COUNT         1358 a         1013 c          968 g         1304 t
ORIGIN      
        1 ttatcgggca aagcaataat gtgaatgaac aaaattttgc agcggacgat atggcagcaa
       61 tgcaaactac aagcggaggc gggatgagag gacacggagg accgggtggc cctccaaggg
      121 gtggtggcga attcggccgt aacaataatc catttatgac caatacctcc ggcggaatcg
      181 ttaccacaca tgctctgggg gtcaactata ccgacaaatg ggctgacaaa gtcgtgtttc
      241 agggaagtgt gtttggcaac cttacgaata ctaacctgat acaatcatca tttacggaat
      301 attttacggg tcagaactca aaggacaata ccgtgtccga tcgcgatggt aaatccttcc
      361 gcttcaacgc acgcgttgag tacaccatcg actctttcca atccgttatc tatacacctt
      421 cgtataacta tggatacaac acaagcaact acatcaacaa ttctgacatc tttacgggtg
      481 aaacacccct gagcaagtat gacaacaagt cttcatccta tggcaaaaat gtatctttca
      541 ataatgagtt gatgtataga aaacggttta gtacacaagg aagaaccatt tctttaaggc
      601 tggaccaatc ttattccaat aatcttccac gaacggatca aaacctgttg actatatctt
      661 atgatactgt caatattgaa atcctggaaa aacaatatgt ggaaagcaac aattataacc
      721 gatcgtatag tgcagaggtc aactatacag agccaatcgc caaggatcat ttcttaatgt
      781 tcggttacaa ctattcaatt aaagataatg atatcgacaa acgactttat gacctgggca
      841 atggtgagca acccggagag cagaatctga tcacttccct gtccaatgtt accgacaatc
      901 tttatcaaag tcatcgaccc agacttgatt atcgctttca aaataaaaaa ctcaatcttt
      961 cagccggtgt gggttatcaa tattctatcc tgaatacgga caagaccttc ccacaagtgg
     1021 aacatgtcaa taaaacattt aaaaatgtat tgccttctgc catggtccgc ctcagcataa
     1081 gcaagactaa gaatttccga ctgttctatc gtgccggcac tcaagagcct tcggtaagcc
     1141 agttgcagag cgtaattgat aatagcaaca ccctccagtt gacatcagga aatcctaccc
     1201 ttgatcaaag tacaacgcat agaatgatga tgttcttctc ctccaccaat caacagaccg
     1261 gctctaactt ttttataggt ggtggtggta tggccacaaa ggattatatc gccagccagg
     1321 tgatatactc cgataaggat acaatactgg cgggttatgg caacatgccg gccggtgcaa
     1381 gatatactta cccgatcaat ctcgacgggt attacagtat ccgtggattt tcatccttcg
     1441 gatttccgtt ttacctgatc aaatccaatg taagccttaa tgtcaatggc tcgatgatca
     1501 acgctccaag caagatcaac agccagatca atcattcacg gtccaagaat gtcggtggcg
     1561 gcgtgacaat ctcttccaat ttcagccaga atctggactt caccatagcc acacaggtag
     1621 gatttagcga tgttaccaac gatatcaaca cgcaagccga caatacttat ttcaatcaga
     1681 atgtgtcagg tcggtttaac tggatcttct tcaagaattt tgtgttcagt acggatgccg
     1741 catattatta caataaggga ctggaaagtg tcagccagac caactacacc atctggaatg
     1801 cagcattcgg ctataaattc ctcaaaaaga atgcagccga aatcagaatt caggcatttg
     1861 atatcttaaa taacaatact gccctgaccc gaaacgtgac tgaaacctat tacgccgatg
     1921 tcagcaccaa cgttttgaac aggtatttca tgctacaatt ctcgtataaa ttctaactgg
     1981 aactcccggc attcatttcc ggattttaaa actatatgtt ccaatcaata caaagagagg
     2041 tcgggtaact acgggcctct cttctatttt atttccgtct cgtaaaatat gatacgatta
     2101 tttgcttatg acaaaaaaat tcattatatt gtcagaaaaa acttatgcag gtaaaggaca
     2161 aaacagttgt aatcaccggt ggcggtaacg gtatgggccg tgaagtagtg cttaacatgt
     2221 tgcataaagg ggcacgagtc gctgctgtcg atgtaaatgg atccgcttta gaagaaacaa
     2281 aaaggcttgc cggcgccaac gctgaccgat tatccatcca tgtgctggat atcacggaca
     2341 aggatgctgt ttttgccttt ccgcaaatcg tcattgcaga gcatggaagc gtggacatcg
     2401 ttatcaataa tgcaggcatc atccaaccct ttgtcagagt caatgatctc ccttatgaca
     2461 agataacccg ggtcttcgat gtcaattttt tcggcctgct ctacatgact aagtcgtttc
     2521 tacctcacct cctggaaagg cctgaagccc acctggtcaa tgtatccagt atgggcggat
     2581 ttcttccagt ccccggccag agtatctatg gtgcaagcaa ggcggcagtc aaattactca
     2641 ccgaggggtt gtatgcagag ctgcgccata cccgtgtcca tgtgagtatt gtatttcccg
     2701 gtgcaatcgg taccgaaata accaggaatt caggggtgga cgttccaaac atggctgccg
     2761 gtgtcgatgc taaaaaacaa gctgccagaa cgacttcgcc cgataaagcc gccgaaatca
     2821 tcgtcaacgg tatccttaaa aataaaaaca ggatttttgt aggaggagat gcctcgtttc
     2881 tggatcggtt ataccgattg gccccgacct ttgcaaccaa tttcattgcc ggtaaaatga
     2941 aggatttgct gaagtaaagg cgggacgtgt ttttattgtt gagttcagcc tgttttgtga
     3001 aatatttgcc gtatcactaa cgcgtataag gcttcggcct gattttataa cttgattcaa
     3061 atgacttttt accatgccga taacaacaga tattcctgaa gggtatgaaa ggataaagaa
     3121 gacgatcgac atttacaggg ctaatattta cggctttttg attctgatcc cggtatctgc
     3181 catgtacctc ttgccatatt atctggtgtg gcaaaaaata ccggctatag gcagtatttt
     3241 atcagatcag ggacatcctc ctatccgtgt attgatctac acgattgtcg cattggtggc
     3301 aggtgttgtt ctccacgagc tgattcatgg aataacctgg tccttcttta ccaggcatgg
     3361 attccggtcg atacgattcg gcatcatgtc taaggcgctg acgccatatt gtcattgcaa
     3421 agaaccgctc agcaagaatc aatacattct cggagccata atgccttcca tcctactcgg
     3481 cgtgattccg ggcatttggg cgattgccat gggtaatgca gttgctttgt attggggtgt
     3541 tttctttacc attgcggcgg tgggagattt catgatcatc agcttgatca ggaaagaagc
     3601 ccggaatgtc ttcattttgg accatcccac cgaagcagga tgttatgtgt tcaggaggaa
     3661 gacgacgccg gaaaaggatg aatccatctt atgaactttt cagtctcatg tgttttcaaa
     3721 aaactttgaa tcaatatctc cgggatatcc aatcaataaa cagtttgagg tactccgaaa
     3781 cttctgttat aaaaaaaagg gcagcttagc agccaaccct ttctcggagt tgatttccac
     3841 tctcttttga gcagcaatca acaaaaattc aggcaaaaaa ttaattcagt tctctatgtt
     3901 cattgaggga tttcaatttc tgggaatagc cggccaacag atgataacaa gactttacgc
     3961 tttttgcatg gtttatgata ttctgatcga gcggtttata accggtgcgt ggtccttctt
     4021 tgctttgatg atcggccctg tagttccaga tggacgctta ataatcagtt cttctattgt
     4081 tatattgcgg tcctttccgg atacttcata gggaccgaaa acctccagtt tgttgccaaa
     4141 agcgcctgcc gccacaaagt atttgccagg cttcagtttt gcaaatcgaa aattaccttc
     4201 cctgtccgta ataactgaag taaccaacga caaatcagtt tcattgagca gacttactgc
     4261 agcatcagag gcaactgttg attgatcata tttgattttt ccgttcaggt taactgtttt
     4321 gattgaagca gcggatggaa gtaaaattac gatggcagca catactacca acaccagtga
     4381 tttcacaaaa atggatttgt cagtcattag atttttcatt ttagaatttt ttaaggttaa
     4441 ttagaatcta tgtcaaaagg aattgtgctc ttttattaat tgacttttgt agtgttgaaa
     4501 cgtatggcat gtagcgaact actacacgat ggtatgtggt tttcgatgaa atcgtactta
     4561 ttcacgatat acgaattaat ttttcatcca aattaaccca agttgcgcat tattcaacaa
     4621 attatgttaa aaaacggtca aag
//
LOCUS       DAOQMC010000183         4631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8811_length_4631_cov_4.148601, whole genome shotgun sequence.
ACCESSION   DAOQMC010000183 DAOQMC010000000
VERSION     DAOQMC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8811_length_4631_cov_4.148601"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKV31_07670"
     CDS             complement(<1..595)
                     /locus_tag="PKV31_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92774.1"
                     /translation="MYYIKRLFFLTLLSVTFITANAQQNQQVSQINAVNNYISFANES
                     DHGLLIIHRLLELYNQEMNKLVNLPHYRLNVFSNADFPENIFEDKSGSFYRVSPNELY
                     AKALKESSALSPKFSSKANEIIGNMKRILDDLEKERYSIGTLVANTDLKRISNLKNVY
                     AALDACVVKFDSYEAERKELAALIARMGPATRKNDSFY"
     gene            764..3049
                     /locus_tag="PKV31_07675"
     CDS             764..3049
                     /locus_tag="PKV31_07675"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNB92775.1"
                     /translation="MLLYSQNRAIDAESGDGLPFCTIVSKHNPDIYTVSDINGNFNLA
                     HTVAGDTIIIRYIGYDTLEAIFLPNKEYRLYPATSMLKEAVIIADDGPAIALIKKVLK
                     RRNDHNILNLDYYQCRIYSRNTIGFDTSADSLDIKQFGKIFRYPASLFISESIIERQY
                     ERKQKMYEKVITSKMSGLRDEKFVILPEDVQSLNFYRDYISIFNKDFVNPISPLSWLK
                     YHFNLDKVYEEANDTLYKIDYWPRNSGYNCFKGMIIISANDWAVQKITMKNTFMDLYP
                     FVLYQEYWRVNNQWFPYKFITEVKLPSAFGKEHPFLVKQTNIFDKITFLPIPIEPSKI
                     NKTEYQTGALPSIDSMRMMPLNRQDSTAYVFGDVLLRSGPSGYILQNMQLILNGQLPI
                     GPLAIDMLSIYRKNLFEGNRFGLSVHSSMKLMPHLGLEAYFAYGTHDNALKYGGSVAW
                     YFDKLRTSNIKYTWNKDVEGNRLFRFRNQWFNQYYSNLFVEIDKHEFSYFKTSASHSI
                     KLATTRETIDPYFEYDFKGINPGESGEYINSEISASYQYIKGRQINFFNTYFIVRNPS
                     YPIFNVSVKSGLKGIAGGNFSYGAIEANFNKIFRWAIIGNTSLTIQGGYIMGRPPIFK
                     LFNAPGARILSMTISTPYSFQTMPPNHYFSTRYLHIFLQQTIRRLYATKFSAPELFVS
                     YNAGWGRLDNMSDHSGVTLKDYHDVFQEVGLGLNSFIRIPIADWFAFGINAGAYYNLN
                     GGLPLEFGKNYNIKTGFGFLF"
     gene            complement(3046..>4631)
                     /locus_tag="PKV31_07680"
     CDS             complement(3046..>4631)
                     /locus_tag="PKV31_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB92776.1"
                     /translation="SKLYNLDTTINNYRFLQISPRFRVKPSTILRYIYLKSGDYYSKA
                     NFTRTYRQLNRLGLFRYINVNQTIDDIDTTLLNVDLYLPPAKTLDISYNLDFGYVNLR
                     TSAVAIPATILGQVGASVIKRNLFNGGEALRTNASAGYETSFNKAVNRIDLKLHNSLS
                     FPRFMDFLGIVSGLKRVKTSKGPLLPKSFIETLEDRVTTNITMDFEYNNFKDWYKYYN
                     LNGSYGFDVIQDNEHQYRWNHLGINYFKPTVFSLYSQVLENNYFLKQSFENPRLFTGL
                     LFRDLSFIYKTADDNYGQNRKFYVNAELSGFEINLINRLINPSTNWKLFDNVEFSKFF
                     KIYTEVSSTKVLFGRVLGAAKIGLGVSHSFGSSTTVPYLKQFEIGGPFSLRAFPIRKI
                     GPGSYIDPVQQEKFPNGPYYQTGDMKIEYLSELRFPLFYIFKGAIFLDAGNIWSISKS
                     DDNRPGSRFRFKDKPWNQLAVGTGVGLRLDLSFFVLRLDMGSALKYPYKINGSYNPYS
                     SFKDRLRNLEYNLALNYPF"
BASE COUNT         1470 a          942 c          858 g         1361 t
ORIGIN      
        1 tgtagaacga atcattcttc ctggtcgcag ggcccatcct ggcaatcaga gccgccaatt
       61 cctttctctc ggcttcataa gaatcgaatt tgaccacaca tgcatccaaa gctgcataga
      121 cattcttcag gttggaaatt ctcttcagat cagtattagc cacaagtgtt ccgatactgt
      181 atcgctcttt ctccaaatca tccaatatcc tcttcatatt gcctatgatc tcattagcct
      241 tagaggaaaa ttttggagat aatgcggaag attctttcag cgcttttgca taaagctcat
      301 ttggactgac ccggtaaaat gatccacttt tatcttcaaa gatattttcc ggaaaatcag
      361 catttgaaaa cacattcagt cgataatgag gaagattgac caatttgttc atctcctgat
      421 tatataattc caataatcgg tggataatca aaagcccatg gtcactttcg ttggcaaatg
      481 agatatagtt gttgactgca ttaatctgtg aaacttgctg attttgctgc gcattagccg
      541 taataaatgt aactgataat aatgtcagaa agaatagacg ttttatgtag tacacgggaa
      601 cttaattttt aatgttcgag ttttataatc tttaaatcat tatttgataa atatatttgc
      661 caaaatccaa tatttataac aaaggaattt gaccacgccc aaaaatacac tatttaattg
      721 tatcgtaaga tataatgtaa tgttattgtt aatgctgcat gggttgttat tatacagtca
      781 gaacagggca atcgatgcag aaagtggaga tggcctccca ttttgtacaa ttgtaagcaa
      841 acataatccg gatatctata cggtatccga tatcaacgga aacttcaatc tggctcatac
      901 agtagctggt gataccatta tcataagata tataggttat gacacgctgg aagccatatt
      961 tctgcctaat aaagagtatc gcctctatcc tgccacctcc atgctaaagg aagctgtaat
     1021 aattgccgat gatggtccgg ccatcgccct gataaaaaag gttttgaaac gtcggaatga
     1081 tcataatatt cttaatctgg actattatca atgcaggatt tactcaagga ataccatcgg
     1141 atttgatact tccgccgact ctttggatat caaacaattc gggaaaatat tccgatatcc
     1201 tgcatctctc ttcatatccg aatcaataat cgaacggcaa tacgaaagga aacaaaagat
     1261 gtatgaaaaa gtgattacct ctaaaatgtc aggccttcgg gatgaaaagt ttgtaatcct
     1321 tccggaagat gtacagagcc tcaattttta tagagattat atttccatct tcaataaaga
     1381 ctttgtcaac cccatttcac ctcttagctg gttaaagtat cattttaacc tcgacaaagt
     1441 ctatgaggaa gccaatgaca cattgtacaa aattgattat tggccaagga attcaggcta
     1501 taattgcttc aaaggaatga tcatcatatc ggccaatgac tgggcagttc aaaaaatcac
     1561 catgaagaac acctttatgg atttgtatcc atttgtatta tatcaggagt attggcgtgt
     1621 caacaaccaa tggttccctt ataaattcat cactgaagtc aagttaccct cagcctttgg
     1681 gaaggaacat ccctttttgg ttaaacagac gaatattttt gataagatca cctttctgcc
     1741 catccccatt gaaccctcta aaatcaataa aacagaatac cagacaggag ccctgccaag
     1801 cattgacagt atgcggatga tgccattgaa caggcaagat agtacagctt atgtatttgg
     1861 agatgtattg ttgcgaagcg gaccatccgg atatatattg cagaatatgc aactaatcct
     1921 gaatggtcag ttaccgatag gcccattagc aatcgacatg ttatctattt acaggaaaaa
     1981 tttatttgaa gggaacaggt tcggactcag cgtccattca agcatgaaac ttatgcccca
     2041 cctgggtttg gaagcatact ttgcatatgg tacacacgac aatgccctga aatacggtgg
     2101 atcggttgcc tggtactttg acaaattaag aaccagtaat atcaaatata cctggaacaa
     2161 agatgttgaa ggtaacagat tgttcagatt cagaaatcaa tggtttaatc aatattatag
     2221 taatttgttt gtcgaaatcg ataaacatga attcagctat ttcaaaacaa gtgcttcaca
     2281 tagcataaaa ctggcgacca caagagaaac cattgatcca tattttgaat acgattttaa
     2341 aggaataaat cccggagagt ccggcgagta tataaatagt gaaatatcag cctcctatca
     2401 atatatcaaa ggcagacaga tcaacttttt caatacttac tttattgtaa gaaacccatc
     2461 ttatcccatc ttcaatgtca gtgtaaaatc cggattgaag ggaatagcag gtggtaattt
     2521 cagttatgga gccattgaag ccaatttcaa taaaattttc agatgggcaa tcatcgggaa
     2581 caccagcctt accatccagg gaggatatat tatgggacgc ccacccatct tcaaactatt
     2641 caacgcaccg ggagccagga ttctttctat gaccatcagc acaccttatt cgtttcagac
     2701 aatgcctccc aaccattact tcagcaccag gtatttacat atattcctac aacaaaccat
     2761 cagaagactt tatgccacca aattctccgc accggaatta ttcgtaagtt acaatgcagg
     2821 ctggggtcgg ctggacaaca tgagtgacca tagtggagtc actctgaagg actatcatga
     2881 tgtatttcag gaagttggtt taggtctcaa ttcattcatt aggattccaa ttgctgattg
     2941 gtttgcattt gggatcaatg ccggagctta ttacaattta aatggtggcc tgccacttga
     3001 attcggcaaa aactacaaca tcaaaacagg atttggcttt ctattttaga acggataatt
     3061 cagggcaagg ttatattcca gattcctaag cctgtccttg aaggaagaat agggattata
     3121 gctaccgtta atcttataag gatactttaa ggcagacccc atgtccaatc tgagtacaaa
     3181 aaaactcagg tcaagcctca aacccactcc tgttccgacc gcaagttgat tccatggctt
     3241 gtctttgaag cgaaaacgag atcccggtct gttgtcatcg cttttgctga tagaccaaat
     3301 attgccggca tccaaaaaaa ttgcaccttt aaaaatataa aataacggaa atcttaattc
     3361 agacaaatat tctattttca tatccccggt ctgataatat ggtccattgg gaaatttttc
     3421 ctgctgcacc ggatcaatgt agctgcccgg cccaatcttt cggatgggaa atgcccgtaa
     3481 actgaaaggt ccaccaatct caaattgttt caaataaggc actgtcgtgc tgctcccaaa
     3541 tgaatggcta acgcccagtc cgatcttggc tgcccctaaa acccggccaa acaacacttt
     3601 tgtcgaagat acctcggtat aaattttaaa aaacttggaa aactcgacat tgtcaaacag
     3661 tttccaattg gttgaagggt taatcaagcg gttaatcagg ttgatttcaa atccggacaa
     3721 ctctgcattg acataaaatt tcctgttctg accataattg tcatcagctg tcttatagat
     3781 gaaggaaaga tccctgaaca ataacccggt gaataaacga ggattttcaa agctctgttt
     3841 taaaaaataa ttattctcaa ggacctgact ataaaggctg aacactgttg gcttgaaata
     3901 attgataccc agatgattcc atctgtattg atgctcgttg tcctggataa catcaaatcc
     3961 ataagaacca tttaaattgt aatacttata ccaatctttg aaattattgt attcaaaatc
     4021 catggtaatg ttggtagtca cccgatcttc aagcgtttca ataaaactct ttggcaatag
     4081 agggccttta cttgttttga ccctcttcaa cccggaaact atccccaaaa agtccatgaa
     4141 ccgtggaaag gacagactat tgtgcaattt gagatctatt ctgtttacag ctttattgaa
     4201 acttgtctca tatcctgcac tggcatttgt ccggagagct tcgcctccat tgaacaggtt
     4261 tcttttaatg acggatgctc ctacctgccc taaaattgtc gcgggaatag caacggcaga
     4321 agttcttaag ttgacatagc cgaaatccag gttgtaagat atatcaagcg ttttggcagg
     4381 cgggagatat agatcaacat tgagcaatgt tgtatcaatg tcatcaatcg tctgattgac
     4441 attgatataa cgaaaaagcc caagtcggtt caattgccgg taagttctgg taaaattagc
     4501 tttggaataa taatcaccac tcttcaaata aatgtatctc agaatagtgg acggcttaac
     4561 ccgaaacctg ggacttatct gcaagaagcg gtaattattg atggttgtat cgagattata
     4621 caatttactg t
//
LOCUS       DAOQMC010000184         4593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_8922_length_4593_cov_4.688189, whole genome shotgun sequence.
ACCESSION   DAOQMC010000184 DAOQMC010000000
VERSION     DAOQMC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8922_length_4593_cov_4.688189"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(457..1332)
                     /locus_tag="PKV31_07685"
     CDS             complement(457..1332)
                     /locus_tag="PKV31_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB92777.1"
                     /translation="MKLTLQTLSAIILLCLLGTSCQKDTSEPSPYLTSQTYNLKDVAY
                     GTDPQQKMDMYLPANRSSSKTKVFVMIHGGGWSAGDKADYSSTMDALKDYYPNHAIIN
                     INYRLGTQDYPGYPKQIQDIQTALNEISKGKYNLSQQYLLFGGSAGAHLSMLYAYAFD
                     HDHHVKGVINTVGPADLTDPSLTNNLILMGVVGGFLGKAIYDYQGNKALFEETSPARR
                     VTPNAPPTLSFYGDKDPLIPVTQMPKLRTALENNHVWHYDTLYPGGGHGLWASDAQTQ
                     DYITRIIAFIDQFFK"
     gene            complement(1851..3293)
                     /locus_tag="PKV31_07690"
     CDS             complement(1851..3293)
                     /locus_tag="PKV31_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92778.1"
                     /translation="MNKSFLPQILIIFLIFSEARSQPVFDTVALIAVEEVNTQFCLNN
                     TKLMAGKSMSDRYLVYYNPDTIFLNILQNGTWIRKIAHTGSNIRSATLTTSYDRIWIC
                     WKEGPFIKASYTTDKGNTWSDDFSVSPPGNVSAPSIHASSNGKVHFVWHTESPSDTSI
                     FYRAFNHDMFTTSPQRLSNQEGHGLWPSVISIGDTVLCTWKEAPLPTKVWFRNSFDGG
                     NTWNPLPPEPTTTALTLTKDPNLAYAFDSTTKTHYTYLAYDGHNKIYLQRSTDFGASW
                     SPPDFISNPGKLSQFAHIDCDNKGFIGISYEQRPIGTSLFNDKNKDVGFTFSSNWGSP
                     GSFSTDTLAYTYNNNGSVYAAFNKIDDNSFFLTWLSKDTIENKTFVFERRIHFNHTTD
                     ILSTDSQLPTIDIYPNPFMSRAFIKSDVPLSNASIYVFNASGQLSLQLKNINGKSIEI
                     DRSNLASGVYLVATVQNNRIIATKKIIVAN"
     gene            complement(3562..4293)
                     /locus_tag="PKV31_07695"
     CDS             complement(3562..4293)
                     /locus_tag="PKV31_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNB92779.1"
                     /translation="MDFNQKDFNYDRWSDVTPLRTEDELRQHVSQYIAKVYGWMFLAL
                     MVTAISGYYVASSEAMINFIVGNKIGFWVVLIGQLFLVGYLSARINKISVQTATALFF
                     IYSALNGLVFGILFLVYTPASLLTVFGITAGTFGVMSAVGYYTKQDLTKFGHIMFFGL
                     IGIIIASIVNFFMQNEMMYWIISYVGVAVFVGLIAYDTQKIKAYALMESEEMRRKGAI
                     LGALALYLDFINLFIYLLRLFGSRR"
     gene            complement(4351..>4593)
                     /locus_tag="PKV31_07700"
     CDS             complement(4351..>4593)
                     /locus_tag="PKV31_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNB92780.1"
                     /translation="MKLGMAHPMGPLQLADFIGLDVCLAILQVLHSGFGNPKYAPCPL
                     LVNMVTAGNLGVKSGMGFYDYSAGIKDAVVVADFVK"
BASE COUNT         1341 a          888 c          980 g         1384 t
ORIGIN      
        1 ctaaacttat tcttgccatt tttcttccac cttcaaatgt cctgatttca ggtgccttcc
       61 ccagattccc tgtcaatcct acgtggtttc ttaaagtgtt catgataata aaaatttaaa
      121 ggttacaaaa tttaagattt acaattttta ttccgtcatt ttctggtgac gtttggtatg
      181 acaaagatgg agggagccga aagaattatc cggtatttaa acgtttgttg tcatttgtaa
      241 acgattataa atgtttgtaa gcaattacaa acacttatac tatgttttga agacttacaa
      301 ctgcttatgg aagacgaatt gattaccctt aactagctgc agtcgtgaat tcaaaatact
      361 ccgaatttta cgatatattt attgccaggg ttgaatttca tggataaatt ttccgacaat
      421 caataaattg agagcggccg tttgtcaagc tatggtttac ttgaaaaact ggtcgataaa
      481 agcaataatt cgggtgatgt aatcctgggt ctgtgcatcg gatgcccaga ggccgtgccc
      541 tccacctgga tagagggtgt catagtgcca gacatgatta ttttctaaag cggtccgcag
      601 tttaggcatt tgggttacgg gaatgagtgg gtccttgtct ccataaaatg atagcgtagg
      661 cggtgcattt ggggtcaccc gcctggccgg acttgtctct tcaaacaagg ccttattgcc
      721 ctgataatca taaattgcct tgcctagaaa accacctact acgcccatca gtattaaatt
      781 attggtcagg gaagggtctg tcaggtcagc gggaccaacg gtgttgatca cgcccttcac
      841 atggtgatcg tggtcgaagg cataggcata gagcatggac aagtgggcac cggctgaccc
      901 tccaaagagg agatattgtt gggataagtt gtatttcccc ttactgattt cattgagggc
      961 ggtctgaatg tcttgtattt gctttgggta tcccggataa tcctgagtgc caagccggta
     1021 attgatgttg atgatggcgt gattgggata atagtccttt aaggcgtcca tcgtcgagga
     1081 gtaatcggct ttatcgcccg cactccagcc accgccatgg atcatcacga acactttggt
     1141 cttgctgcta ctacggttgg caggtaagta catatccatc ttttgttgag gatcggtgcc
     1201 ataggctaca tccttcaggt tgtaagtttg ggaagtaaga taaggagatg gttcacttgt
     1261 gtccttttgg caggaagtgc cgagcaggca taagagtata atggccgaaa gtgtctgcaa
     1321 tgtaagtttc atagaataat atttcttgtt gacgcaggag tgataaatga caaacgaagc
     1381 attacccttt ttccgtattc aaagttaata gtttatcttt caatttctgt aatatttatc
     1441 aagtaaacca gaacttagat ccatctatca aattcaactt ttagggcaat aggaatatga
     1501 atcaattttt tcaattgagg gttaggcact cccatctgaa atggataatt gtgtattatg
     1561 ttcctcgacc gaaagttgaa aaatttgagt cattgaaccg agaaacaaag atatgcaata
     1621 gggtaatttt tctcaagagg gattagcaaa atagattgat atacctaaga atagcggatt
     1681 tcataagcat ttaaatcaaa tgtaagataa tggtacattt ccttaagata tactgactac
     1741 aatattttat caataatttg aactcagggt tattgttgca attttttcaa aggtttgaat
     1801 cattggattc gtctttatga tcatgtcaaa tttttgtacc tcattattct tcaatttgca
     1861 acaattattt tttttgttgc aataatacga ttgttctgta cagtagcgac caggtatacc
     1921 ccactggcga gattactacg atcaatttca atggattttc cattgatatt ctttaactgc
     1981 aaggaaagtt gaccggatgc attgaaaacg taaatggatg cattgcttaa aggaacgtcc
     2041 gatttaataa aagcacgtga catgaagggg ttgggatata tgtcaatggt cggcaattgg
     2101 gaatctgtag ataaaatatc ggttgtgtgg ttgaaatgga tccgtctttc aaagacaaac
     2161 gttttatttt cgatggtatc tttcgagagc catgtcagga agaaactatt gtcatcgatc
     2221 ttattgaatg cggcatagac agagccgtta ttattgtagg tatatgccaa cgtatctgtg
     2281 ctgaagctgc ccggacttcc ccagttggat gagaaggtaa atccgacatc cttgttttta
     2341 tcgttaaaga gcgaagtgcc aatcggcctt tgttcgtacg aaatgcctat aaagcctttg
     2401 ttatcgcaat caatatgtgc aaattgagat aatttgccgg ggttgctgat aaagtcagga
     2461 ggagaccatg aagcaccgaa atcggtcgaa cgttgtaagt atatcttatt atgaccgtca
     2521 taggcaaggt aggtgtagtg agttttggtt gtcgagtcga atgcataggc aagattggga
     2581 tcctttgtca atgtaagtgc tgttgtagtt ggttcgggtg gaagcggatt ccaggtgttg
     2641 ccaccatcga atgaatttct gaaccagact ttagtaggca atggagcttc cttccaggta
     2701 cataaaacag tgtcaccaat cgagatgact gaaggccata gaccatgccc ctcctgatta
     2761 ctcaggcgtt gcggagatgt tgtaaacata tcatggttga aggctctgta aaatatgctt
     2821 gtatcagatg gggattcggt atgccaaaca aaatgtacct ttccattgga tgatgcatga
     2881 atggaggggg cggatacatt gcccggagga gacaccgaaa aatcatcact ccaggtattg
     2941 cctttatccg ttgtgtagct tgcctttatg aaaggacctt ccttccagca tatccagata
     3001 cggtcatagg atgtggtcag cgttgctgat ctgatattgc ttccggtatg tgctatcttc
     3061 ctgatccagg ttccgttctg tagaatattc agaaaaatgg tatccggatt ataatatacc
     3121 agatatcggt cggacatgga ttttccggcc atcaatttgg tgttgttgag acaaaattgt
     3181 gtatttactt cttctacggc gatcaaggcg acggtgtcaa aaacaggctg acttcttgcc
     3241 tcagagaata ttaaaaaaat aatcaatatt tggggtaaaa atgatttatt catgatgtgt
     3301 ttagtttaat ctaagcattt gacgctacca agcgcagttg gtttaaatat ctgatttgca
     3361 cgatccgatc gtttgatgca taacatatca aaatgccgga tagacctgga acagacagac
     3421 ccgtcctgtt tttttattac ggatcataaa attattcggt aaaccggccc tgtgtgttct
     3481 gtcttttgta tagcaacctt tctaaacaaa aaaggccatc cgacttaacg gatggccttg
     3541 ataattttta aaatcaactt atcaacgtct actaccaaat aaacgtaaca gataaataaa
     3601 taagttgata aaatcgaggt agagggcaag tgcgcctaaa atcgcacctt tcctgcgcat
     3661 ttcttcactt tccatcagag catatgcctt gatcttctgt gtgtcgtaag ctatcaatcc
     3721 gacgaatact gccactccca catacgatat aatccaatac atcatctcat tctgcatgaa
     3781 gaagttgacg atggaggcaa taatgatgcc aataagaccg aagaacatta tatggccaaa
     3841 cttggtcaaa tcctgcttgg tataatatcc cacggcactc attaccccaa aagtgccggc
     3901 cgtaatgccg aaaactgtca gcaatgatgc aggtgtatat acgagaaaaa gaatgccaaa
     3961 tacaaggcca tttagggcag agtaaataaa aaacaaggcg gtagcggtct gcacactgat
     4021 tttattaatc cttgcactca aatagccaac taaaaaaagc tgaccgatca gcactaccca
     4081 gaatcctatt ttgttcccta caatgaaatt aatcatagcc tcgctggagg ctacataata
     4141 tccgcttatt gcagttacca tcagtgccag aaacatccag ccatatacct tggcaatgta
     4201 ctgagataca tgctgtctga gttcatcctc tgttctcagc ggggtaacat cagaccatct
     4261 gtcataatta aagtcctttt gattaaaatc cattttttgt tttttattat taatactaat
     4321 ttatgctaaa aagttaaaag atccaacata ttatttcaca aaatctgcaa caactacagc
     4381 atcttttatc cctgcactat agtcgtaaaa gcccatgccg gacttcacgc ccagattacc
     4441 ggcggtgacc atattgacca aaagcgggca tggagcgtat tttggattgc cgaagcctga
     4501 atgcaatacc tgaaggatgg cgagacatac atccaatcct ataaagtctg ccaactgaag
     4561 cggccccata ggatgtgcca tgcccagttt cat
//
LOCUS       DAOQMC010000185         4551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9075_length_4551_cov_4.354982, whole genome shotgun sequence.
ACCESSION   DAOQMC010000185 DAOQMC010000000
VERSION     DAOQMC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9075_length_4551_cov_4.354982"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PKV31_07705"
     CDS             <1..484
                     /locus_tag="PKV31_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB92781.1"
                     /translation="LQSWLDAGNDVGLMSESGCPGIADPGAEVIQWAHRSGYEVVPLV
                     GPSSILLALMASGFNGQSFVFHGYLPNKKEELVKKVKLLEQNAGRLNQSQIFIETPYR
                     NLTMYDILVSTLQPNTHLSIHMGLTGPHAYSKSKTVKEWRKLGCSELRKDIPAIYIIG
                     "
     gene            815..1783
                     /locus_tag="PKV31_07710"
     CDS             815..1783
                     /locus_tag="PKV31_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNB92782.1"
                     /translation="MLINAGILNAIGEKVEYRQVEIHPQGKEVIVDLMASSLNRRDLW
                     IQEGKYARIKLPCIPGSDGAGRYEGRDIILLPTINWGNSPAFQSAQFEILGMPHHGCC
                     AEKIAVHPSLIYPMPSHLDYNEAASLPLSGLTAWRALMTQAQAKPGQNILITGIGGGV
                     ASFVLQFAVAFGMHVYVTSGSDDKIIMAKSYGAKGGANYRNPEAFSELQTMAQGFDII
                     IDSAGGADFHKLLKLANFGAHIVIYGGTKGNIDSINPQNLFWKQLTIKGSTMGTPEEF
                     NDMLDFVNEHKVKPIIDKIFPLNQINEAFDYMISGSHFGKIVINHL"
     gene            1780..2280
                     /locus_tag="PKV31_07715"
     CDS             1780..2280
                     /locus_tag="PKV31_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNB92783.1"
                     /translation="MRVALLSDTHGYIDPLWPEYFMSCDEIWHAGDIGTIEVLDHLKS
                     FKPVRAVFGNIDGYVIRAELPETMEFEVEGLKVLMTHIGGYPNKYESRARRLIEFYRP
                     GLFICGHSHILRVIYDKKFNLLAMNPGASGLHGFHKVKTMLRFSISEGKVHDAEVIEL
                     GSKRLD"
     gene            complement(2277..3359)
                     /locus_tag="PKV31_07720"
     CDS             complement(2277..3359)
                     /locus_tag="PKV31_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92784.1"
                     /translation="MIIRNFSILFFLVISLVVNGQQKQKQTLHYKQEKHLKKVQQLTF
                     GGDNAEAYWSFDNKSLVFQSNNPAWGLSCDQIFIMPAKEMKPNKSGVRLTPTMVSTGK
                     GRTTCSYFMPDNKSVVYASTHESMDNCPPEAPHTGKYVWMLYPEFDIYQADLKGKIIK
                     KLTNEPGYDAEATVSPKGDKIVFTSTRTGDLELFTMNIDGSDVRQVTHDLGYDGGAFF
                     SPDGTKLIFRASRPKTDAEIKEYKELLAQNLVQPTAMELFICNVDGSDLRQITNLGKA
                     NWAPYMHPDGKSVLFSSNHHSPKGFPFNIFKINIDGTGLEQITFDNTFDSFPMFSPDG
                     KKVSFSSNRNNGGTRDTNVFIAKWKD"
     gene            3442..>4551
                     /locus_tag="PKV31_07725"
     CDS             3442..>4551
                     /locus_tag="PKV31_07725"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB92785.1"
                     /translation="MMKKGILFILILFNCLYVNGQEVALSKRFHSDISFLASDAMKGR
                     LTGSIFQDIAAEYIASRFLQLGLKPMGDQNTYFQTFYYVPTVDPHSSANLHLESGDSA
                     KITNVIGYIDNGAQSTLVIGAHYDHLGDGHSSFSLSTEAGIHNGADDNASGVSMMMEL
                     AQALKNSKSKYNFLFIAFSGEEFGLWGSNHFVKNPTIDLKKVATMINLDMVGRLKDDN
                     SLLIGGVGTSPVWKGLLEKNNVSKLKLIFDESGSGPSDHTSFYFQNIPVLFYFTGQHE
                     DYHKVSDDTEKINFDGMTKIYNNILALVRSLEKVNEIPFTATKDDKQEKVVMKVTLGI
                     MPDYMYNEKGLRIDGVKEDKPASKAGLKAGDVINPD"
BASE COUNT         1384 a          884 c          931 g         1352 t
ORIGIN      
        1 gcttcaatca tggttggacg ccgggaacga tgtcggttta atgtctgaat caggttgtcc
       61 cggcattgcc gaccctggtg ccgaagtcat ccaatgggca catcggtccg ggtatgaagt
      121 tgtaccgtta gtcgggccca gttcaattct acttgcactt atggcaagtg gatttaacgg
      181 tcaaagcttt gttttccatg gttatctacc taataaaaaa gaagaactgg tcaaaaaggt
      241 taaattactg gaacaaaatg cgggcagatt gaatcaatct cagatattca ttgagacgcc
      301 ttatcgcaat cttacaatgt atgatatact tgtatcgaca cttcaaccaa atactcacct
      361 ttctattcat atgggattga cgggcccaca cgcatattct aaaagtaaga cagtcaagga
      421 atggcggaaa cttgggtgtt cagaactcag gaaagatatt ccggccattt atatcattgg
      481 gtagggtaat aatctgaatg tcaatgcaat gtgggttaat aatacgaagt aggtagatga
      541 ctaatacttg accacctcat ccttaaagca tttcgtaatt attttaaatc agttacgact
      601 gatatagagg aaaatatttt tcctcatcag ttcaatcaga ggactgattt gtagttacag
      661 tattaaatat ctacctactt gaatttcccg aaagtatcaa cttccagacc tgacttttat
      721 cattacttaa acgcatgatt aagtatctca tctacaaaca tccggtttaa attatcatga
      781 attcattact tttaccttct tacaaattaa cattatgctc atcaatgcag ggatcttaaa
      841 cgccatcggc gaaaaagtag aatatagaca ggttgaaatt catcctcaag gaaaggaagt
      901 aatcgtagat ctaatggcaa gttcactgaa cagacgggat ttatggattc aggaaggtaa
      961 atatgcaaga ataaagcttc cctgtattcc cggttctgat ggtgcaggac gatatgaagg
     1021 ccgtgatatt attctgttgc cgaccataaa ttggggaaac tcccctgcat ttcaatctgc
     1081 tcagtttgag attctgggta tgccgcatca tggctgctgt gctgagaaaa ttgctgtcca
     1141 tccatcacta atctatccca tgccgagtca cctcgattat aatgaagcag cgtcccttcc
     1201 actttccggt ctaacggcat ggagagctct catgacccag gctcaagcca agccgggaca
     1261 aaacatttta attacaggta taggcggtgg tgtggcaagc ttcgtgctac aatttgctgt
     1321 ggcatttggc atgcatgtat atgtaacaag cggctctgac gataaaatca ttatggccaa
     1381 atcatacggt gctaaaggtg gcgccaatta tcgtaatcct gaggcgtttt ctgaattgca
     1441 gacaatggca cagggatttg atataataat tgattccgcg ggtggtgctg actttcataa
     1501 attattgaaa ttggccaatt ttggtgctca catagttatt tacggtggca caaaaggaaa
     1561 tattgattcc atcaatcctc agaatttatt ctggaaacaa ttgacaataa aaggttcaac
     1621 tatgggaaca ccggaggaat tcaatgatat gctagatttc gtcaacgaac ataaagtaaa
     1681 gcctattatt gataagatct tcccattgaa tcaaatcaat gaggcatttg actatatgat
     1741 ttcaggaagc cactttggca aaatcgtcat caaccattta tgagagtggc acttctttct
     1801 gatacacatg gctacatcga cccattgtgg cctgaatatt ttatgtcatg tgatgaaata
     1861 tggcatgccg gagacatcgg cactatagaa gtgctggatc atttaaagag tttcaaaccg
     1921 gtgcgggcag tgtttggcaa cattgacgga tatgtaatcc gggcagaatt gccggaaacc
     1981 atggagtttg aagtcgaagg acttaaagtg ctgatgaccc acattggggg ttaccctaat
     2041 aaatatgaaa gccgggcacg tagactcatt gagttttaca ggcccggcct ttttatttgt
     2101 ggacattcac acatactacg tgtgatttat gacaagaagt tcaacttatt ggcgatgaat
     2161 ccgggtgcgt ccggcctcca tggctttcat aaggtcaaga ctatgcttcg attttctatt
     2221 tctgagggaa aagttcatga cgcagaagtc attgagctgg gaagcaaacg tctggattaa
     2281 tccttccact ttgcaataaa gacattcgta tctcttgtac cgccattgtt tctattggaa
     2341 ctgaaggaaa ccttcttacc atcaggagaa aacataggaa aagaatcgaa agtattatca
     2401 aaagttattt gttccaagcc ggtaccatca atattgattt taaagatatt aaaaggaaat
     2461 cctttggggc tatgatggtt actagaaaac aatacgcttt tgccatccgg gtgcatatat
     2521 ggcgcccaat tagccttacc aagatttgta atttgtcgca aatcacttcc atctacatta
     2581 cagataaata attccattgc ggtaggttga accaaatttt gtgccagcaa ttccttatat
     2641 tcctttattt ctgcatctgt tttaggccta cttgcgcgga aaatcaattt tgtgccatcc
     2701 ggagagaaaa atgcaccacc gtcataacca agatcgtgtg ttacctgtct gacatcagaa
     2761 ccatcgatgt tcatggtaaa aagttctaaa tccccggtac gcgtgcttgt aaatacaatt
     2821 ttatcacctt taggagagac agtggcttca gcatcatatc caggttcatt ggttagtttc
     2881 ttgataattt taccctttaa gtcagcctgg taaatatcaa attcaggata taacatccat
     2941 acgtacttac cggtatgagg ggcttcagga ggacaattat ccattgattc gtgtgtactg
     3001 gcataaacaa cacttttgtt gtccggcata aaataagaac aagtagtccg gcccttacct
     3061 gtacttacca tagtaggagt caacctgact ccggatttgt taggcttcat ttccttggca
     3121 ggcataataa aaatctggtc gcatgaaagt ccccatgcag gattattgga ttgaaagaca
     3181 agagacttat tgtcaaaaga ccaataagct tccgcattat ctccgccaaa agtcaattgc
     3241 tgaactttct taagatgctt ctcctgctta tagtgcaaag tttgtttttg cttctgctgg
     3301 ccattaacaa ccaggcttat aactaaaaaa aatagaatgg aaaaatttct tattatcatg
     3361 ataaaatttt tatttgttat acgttttgta cctttgcggg gctttaaaaa tgactgcaaa
     3421 tatcgtaatt aaataaagaa catgatgaaa aaaggtatct tatttatatt aattctattt
     3481 aactgcttat atgtcaatgg acaggaagtt gctttaagta agagatttca ttccgacatt
     3541 agctttttag cttctgatgc catgaaaggg cgtttgacag gcagtatttt tcaggatatt
     3601 gccgcggaat atatagccag tcggtttctt cagctgggat tgaagccgat gggcgatcaa
     3661 aatacctatt ttcagacttt ttactatgta cccacagttg atccacatag ttctgccaat
     3721 ctgcatctgg aatcaggtga ttcagccaag ataaccaatg taataggata tattgataac
     3781 ggtgcccaat ccactcttgt aattggagca cattatgacc accttggtga tggtcacagt
     3841 agtttcagtc tttccacaga agccggtatt cataacggtg ccgatgataa tgcctccggt
     3901 gtcagcatga tgatggagtt ggctcaggca ttaaaaaatt ctaaatcgaa atacaatttc
     3961 ctgttcattg ctttttcggg cgaggaattt ggtctttggg gttcaaatca ttttgtgaaa
     4021 aacccaacca ttgatctgaa gaaagtagca accatgatca atttggatat ggttggacgg
     4081 ctgaaagatg acaatagcct tttgataggt ggcgttggca cctctcctgt ctggaaaggc
     4141 cttctcgaga aaaacaatgt atccaaatta aagttaatat tcgacgaatc cggttcaggt
     4201 ccctcagatc atacatcttt ttacttccaa aatattccgg tactgtttta cttcaccggg
     4261 caacatgaag actatcataa agtttctgat gataccgaaa aaatcaactt tgatggtatg
     4321 acaaagatat ataacaatat ccttgcattg gtcagatcat tggaaaaagt caatgaaatt
     4381 ccttttacgg caaccaaaga tgataaacag gaaaaagtcg tcatgaaggt aaccttaggc
     4441 attatgccag actatatgta taatgaaaaa gggcttagaa ttgatggagt caaagaagac
     4501 aaaccggcct ccaaggccgg actcaaagcc ggcgatgtta ttaaccccga t
//
LOCUS       DAOQMC010000186         4550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9077_length_4550_cov_4.925695, whole genome shotgun sequence.
ACCESSION   DAOQMC010000186 DAOQMC010000000
VERSION     DAOQMC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9077_length_4550_cov_4.925695"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2700
                     /gene="purL"
                     /locus_tag="PKV31_07730"
                     /gene_synonym="purI"
     CDS             <1..2700
                     /gene="purL"
                     /locus_tag="PKV31_07730"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNB92786.1"
                     /translation="ASNGASDFGNKFGQPLICGSVLTFEHKEADRTLGYDKVIMQAGG
                     IGFGKAGQAKKHIPAAGDKIIVMGGENYRIGMGGAAVSSADTGEFGSGIELNAVQRSN
                     PEMQKRVANAIRGMVESDHNHILSIHDHGAGGHLNCLSELVEQTGGFIDLDKLPVGDP
                     TLSAREIIGNESQERMGLVIADSHIDTLNKVAERERAPMYIVGKVSNDHRFTIASSST
                     GARPMDLKLGDMFGSSPKTIMEDKTIDRQYSPLIYDRKRIDEYLESVLRLEAVACKDW
                     LTNKVDRCVGGLVAVQQCAGPLQLPLNNCGVMALDYSGRDGIATSVGHSPLTALIDSA
                     AGSRNAIAEALTNIVWAPLQDGIRSISLSANWMWPCKNEGEDARLYNAVRACSDFAIA
                     LGINIPTGKDSLSMKQKYPEGEVLAPGTVIISAAGHCNDVRKVVGPVLKKEDAGIYYI
                     NFSGMSPRLGGSSFAQVLNRVGDTAPDIADASEFVEKFAVIQSLVSQGLISAGHDIGS
                     GGLITTLLEMCFADRGLGAEIDLSALPGEDIIHKLFAENAGVVIQASSVAEEILIGAG
                     VEFFPIGKIRREATLQVRDHEDSHTFDIDRLRDIWFETSYLLDIRQSGPKKAKERYDN
                     YKHHLLHYKFPASFDGKLPHIDSARPRPKAAVLREKGSNSERELANALYSAGFDVKDV
                     HMTDLISGRETLDDIQMIGAVGGFSNSDVLGSAKGWAGAFLYNEKARSALERFFARED
                     TLSVGVCNGCQLFIELGLINPDHEVKPKMRHNTSEKHECIFTTLTIGDNNSVMLSSLA
                     GSTLGVWVSHGEGRFSLPYEENKYHIVAKYAYDTYPASPNGSDYNTAMMCDVSGRHLV
                     MMPHIERSFFQWNWPYYPEGRNDEVSPWVEAFVNARKWIEARE"
     gene            2803..4245
                     /locus_tag="PKV31_07735"
     CDS             2803..4245
                     /locus_tag="PKV31_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HNB92787.1"
                     /translation="MRKILLFVSFALAVLIGILTFNYFSISSRQTAPTHEKADISSIV
                     GAEERLSRAITFPTVSFEDSTDADYTPFVAFHKYLQSTFPAIYENLEVENINEFSLLF
                     HWKGSDSNLKPVILMAHQDVVPVSEDSKNLWTVHPFEGTIKDGFLWGRGSIDDKSNLM
                     GQLEAVNFLLGLQFRPRRDIYLAFGHDEELGGENGAMKIAQLLKSRGIEAEFVLDEGG
                     IVTDNMVPGVRQPVALIGTSEKGYLDLTLSVRIEGGHSSMPKKDNAIGVISKALTNLS
                     DHPFPPQLSPSVRDFFSYVGPHSSFINRLAISNMWLFKPVLFKMIGKSAPGDAMIRTT
                     MVPTIIHGGDKSNVVPDIATANVNLRLLPGTTIPDAIDHVRKVINNPAVKIEALGEQK
                     EAGIVSPADNDAFKYISDAVHKNFPNTLVSPFLMIGGTDSKHFEILSQNIYKFSPLID
                     PIGFHGVDERLNLKAYPQAIGFYVDLIKSL"
     gene            4313..>4550
                     /locus_tag="PKV31_07740"
     CDS             4313..>4550
                     /locus_tag="PKV31_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92788.1"
                     /translation="MDWHPVLILRILMAAFSEFLTLVNLIKKRGVNKNLKKISAGVSE
                     EKNTYIDEFPEPIKSVAMTIAEQLYRKGVKEGKEQ"
BASE COUNT         1266 a          975 c         1112 g         1197 t
ORIGIN      
        1 gcttccaatg gcgcctctga ctttggaaac aagttcggac aacccctaat ctgtggttcc
       61 gtgctaacat ttgaacacaa ggaagcagat cgtacccttg gttatgacaa ggttatcatg
      121 caagccggag gcattggttt tgggaaggcg ggacaggcaa aaaagcacat tccggcggca
      181 ggtgataaga tcatcgtcat gggtggcgaa aactaccgta ttggtatggg cggagcggct
      241 gtctcctccg ctgatacagg agaatttggc tccggcatag aacttaatgc tgtgcagcgg
      301 tctaaccctg aaatgcagaa acgagtagcc aacgccatcc gaggtatggt ggaaagcgat
      361 cataatcaca tcctgtccat acatgatcat ggagcaggcg gtcatctcaa ctgcttgtct
      421 gaactggttg aacaaaccgg aggatttatc gaccttgata aactgcctgt tggagatcca
      481 accctatctg cgcgtgaaat cattggcaac gaatcacagg aacgaatggg cctggtgatt
      541 gctgacagtc atatcgacac cctcaacaaa gttgccgaac gagaaagagc tcccatgtat
      601 attgtaggca aagtcagcaa tgatcatcgc tttaccatag cttcctcatc taccggtgca
      661 agaccgatgg atctgaaact tggtgacatg ttcggcagct caccaaagac catcatggaa
      721 gacaagacca tcgacaggca atattcaccc ctcatatatg acaggaaaag aattgacgaa
      781 tatctggaga gtgtgctgcg tcttgaggcc gtggcgtgta aggactggct gaccaataag
      841 gtggatcgct gtgtcggagg cttggtggca gtccagcaat gtgccggacc acttcagcta
      901 ccgctaaaca attgtggagt gatggcactg gattattccg gccgtgacgg gattgccaca
      961 tcggtggggc attcaccact gacagcactg attgattctg ctgcagggag cagaaatgcc
     1021 atcgccgaag cattgaccaa tattgtctgg gccccgctgc aggatggcat ccgaagcata
     1081 tcgctctctg ccaattggat gtggccttgt aagaatgaag gcgaggacgc acgactgtac
     1141 aacgcagtca gggcttgttc ggactttgcc atcgccttgg gaatcaacat acctaccgga
     1201 aaagactctc tctctatgaa gcagaaatat cccgaaggag aggtcttggc acccggtact
     1261 gtcattattt cagcagccgg acattgtaat gatgtgagaa aggtcgttgg acccgtcctg
     1321 aaaaaagagg atgccgggat ctattatatc aatttttccg gaatgtcacc ccggctggga
     1381 ggttcttcat tcgctcaggt attgaacaga gtaggagata ccgctccgga cattgcagat
     1441 gcatccgaat ttgttgaaaa atttgctgtt attcagagtc tggtcagtca ggggttgatt
     1501 tctgcgggcc atgacattgg aagcggaggc ttgattacca ccttgctgga aatgtgcttc
     1561 gctgaccgtg gactaggggc tgaaatcgac ctttcagcgc ttcccggtga agacatcatc
     1621 cataaattgt ttgcagaaaa tgcaggagta gtcatacagg catcctcagt tgctgaagaa
     1681 atattgattg gtgccggagt tgaatttttc ccaataggca aaataagaag agaagccact
     1741 ttgcaggtca gggatcatga agattctcac acttttgata tcgacagatt gcgtgacatc
     1801 tggttcgaaa catcctatct gctggatatc agacaaagtg gaccgaagaa ggcaaaggag
     1861 cgatatgaca actataagca tcatttgttg cattataaat tcccggcttc ttttgatgga
     1921 aaattgccac acattgattc tgccaggcca cgcccaaagg ctgctgttct ccgggaaaag
     1981 gggagtaatt cggaacggga gctggccaat gcactttact cagccggatt tgatgtgaag
     2041 gatgtgcaca tgaccgattt gatcagtgga cgtgaaaccc tggatgatat tcagatgatt
     2101 ggcgctgtag gtggattttc aaattctgat gtacttggtt ctgcaaaagg ctgggccggc
     2161 gcgtttttgt ataacgaaaa agccaggagt gccctggagc gcttctttgc ccgggaagat
     2221 accctgtctg taggcgtgtg caacgggtgc cagttattca tcgaactggg tcttatcaac
     2281 ccggatcacg aagtaaagcc taaaatgcgt cacaatacta gtgagaagca cgaatgtata
     2341 tttaccaccc tgaccatcgg tgataataat tccgtcatgt tgtcatccct tgcaggatct
     2401 accctcggtg tttgggtgtc acacggagaa ggtcgattca gtttgccgta tgaagaaaac
     2461 aaataccata tcgtagccaa atatgcttat gacacttatc cggcaagccc caatggctca
     2521 gattacaata cggctatgat gtgtgatgtc tcaggccgcc atctggtcat gatgcctcat
     2581 atcgaacgtt ccttcttcca gtggaactgg ccttattatc ccgaaggacg taatgatgaa
     2641 gtgtctccgt gggtagaagc cttcgtcaat gccaggaaat ggattgaagc cagagaatga
     2701 agtaaaagtg aaaaggaata gatttttata gggttagtta gccttcgaat tttatcaaat
     2761 tattcgtata ttcgatactt tttcgatgct gatataccgt ttatgagaaa aattctgctc
     2821 tttgtatcct ttgcacttgc tgtattgatc ggtattctta cattcaatta tttttccata
     2881 tcgtccaggc agactgcccc aactcatgaa aaggcggata tatcttccat agtaggcgcc
     2941 gaagaaaggt tgtccagggc gatcacattt ccgactgtct catttgaaga tagtacagat
     3001 gcagactata cgccattcgt tgcattccac aaatatttac agtcaacctt tccggcaata
     3061 tatgaaaatc tggaagtcga aaatatcaat gaattttcac tgctttttca ttggaaaggt
     3121 agcgatagta atctaaagcc ggtaatacta atggctcatc aggatgtcgt tccggtgtca
     3181 gaggattcaa aaaacctttg gacagttcat ccgtttgaag gtaccatcaa ggatggattt
     3241 ttatggggca gaggttctat tgatgacaag tccaacctaa tgggccagtt agaagcagtc
     3301 aatttcttgt taggcctgca attcaggccc aggagggata tctaccttgc ctttggccat
     3361 gacgaggaat tgggtggtga aaatggcgcc atgaaaatcg ctcaactgtt gaaatccaga
     3421 ggcattgaag cagagtttgt actggatgaa ggcggaatcg tcactgacaa tatggtgccg
     3481 ggtgtaaggc aacctgttgc ccttataggt acttcggaaa aaggttatct tgacttaacc
     3541 ctttctgtcc gcatcgaagg cggccactca tccatgccga agaaggataa tgccattggg
     3601 gtaataagca aggcacttac caacctgtcg gatcatcctt ttcctcctca attgtcacca
     3661 tcggtaaggg attttttcag ttatgtaggc ccgcactcct catttatcaa cagacttgcc
     3721 atcagtaata tgtggttatt caaaccggtg ttgttcaaga tgattggaaa gtcagctccc
     3781 ggggatgcca tgatccggac caccatggta ccgacgatta tccatggtgg tgacaaatca
     3841 aatgttgtac ccgatattgc tactgccaat gtcaatcttc gtttgttgcc cggtacaacc
     3901 attccggatg ccatagatca tgtccggaag gtcataaata atccggctgt taaaatagag
     3961 gcgttgggcg aacaaaaaga agccggtatc gtttctccgg cagacaatga tgcctttaaa
     4021 tatatatccg atgctgtgca taaaaacttt cctaatacac tggtttcgcc attcctgatg
     4081 attggaggca ccgattcaaa gcattttgaa atactgtcgc aaaatattta taaatttagt
     4141 ccactgatcg atccgattgg ttttcatggt gttgacgaaa ggctcaatct gaaagcctat
     4201 ccccaggcaa tcggatttta tgtcgatctc atcaagtctt tgtgaaagta cttaatacaa
     4261 taagagatca atgcaatgat ccacatccgg tcagtgattg aatgagattt gaatggattg
     4321 gcatccggta ttgatcctgc ggatcttgat ggccgcattt tctgaattct tgactttggt
     4381 caatttgatt aagaagaggg gagtaaataa aaatctaaaa aagataagcg ccggtgtctc
     4441 tgaagaaaag aatacctata tagatgaatt tccggaaccc ataaaatcag tagcaatgac
     4501 catcgccgaa caattatatc gcaaaggagt aaaggaaggg aaagaacaag
//
LOCUS       DAOQMC010000187         4527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9148_length_4527_cov_3.977639, whole genome shotgun sequence.
ACCESSION   DAOQMC010000187 DAOQMC010000000
VERSION     DAOQMC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9148_length_4527_cov_3.977639"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PKV31_07745"
     CDS             <1..1271
                     /locus_tag="PKV31_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB92789.1"
                     /translation="TLREEIEELDRKAQKAERESDFETVAKIRYGELQAKQQELKESE
                     NELNALPEGSRFTAQMVTANDIADVVSKWTGIPVSKLQQSEKDKLLNMEKEIGKMLIG
                     QTEAVEAVSEAVRRNRAGLSDSKRPVGSFIFMGPTGVGKTELTKALADVLFDDKDAII
                     RIDMSEYMEKHSVSRLVGAPPGYVGYEEGGQLTEAVRHRPYSIVLLDEIEKANADVFN
                     ILLQVLDDGRLTDGKGREVNFKNTIIIMTSNLGSNIILENFEDLEEIEPKRRSEVIET
                     TKLEVFELLKQTMRPEFLNRIDETIMFLPLTKEEIGKILDIQMKGINKLLKSQELRIE
                     LAPSASKLLIDLGYDPQFGARPLKRVLQKELVNELSKQLLSGDYLPGDVIVAEGNNTG
                     LTFHKKKEVMTEPVTENHTDHTDSGRSEEK"
     gene            1452..2447
                     /gene="dusB"
                     /locus_tag="PKV31_07750"
     CDS             1452..2447
                     /gene="dusB"
                     /locus_tag="PKV31_07750"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNB92790.1"
                     /translation="MVKIGGISLGEFPLLLAPMEDVSDPPFRALCKAQGCDMLYTEFV
                     SVEGLIRNADKSVQKLDISDEERPVGIQIFGSEKESMIQAAEIIERTQPEVLDINYGC
                     PVKKVVCKMAGAGILQDIDRMVSLTEAVVKATSLPVTVKTRLGWDEDSKNIVEVAERL
                     QDIGIKALSIHGRTRKQMYTGEADWTLIAKVKENPRIHIPVFGNGDIDSPEKAKLYKE
                     RYGVDGIMIGRASIGNPWIFREIKQYLKDGTIPDPPSLDERIEAVLQHLHRSIMWKGD
                     KVGINEMKRHYGNYFRGYREVKQFRTLLVHSSSFDEVVEITEKMREYYAGEELTV"
     gene            2459..3892
                     /locus_tag="PKV31_07755"
     CDS             2459..3892
                     /locus_tag="PKV31_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB92791.1"
                     /translation="MFLLLNRSENEEFLYKILARAASDLDLQTYLVGGYVRDLILGRN
                     STDIDVVCVGDGIQLAEQVNKLLGNKQPIVVYKNFGTAALRYNDIQIEFVGARKESYQ
                     KNSRKPAVIAGTLYDDQLRRDFTINALAISLNTDTYGELLDPFDGMEHLEEKLIKTPL
                     DPEDTFSDDPLRMMRAIRFSNQLNFSIDPLTYAAINHNKDRLKIVSIERIVTEFQKIM
                     ACDKPSIGIKLLFDTGLLEVFLPELVELQGVEYQDGKGHKDNFYHTLEVLDNVCRNSD
                     NIWLRWAALLHDIAKPATKRFDEKQGWTFHGHDALGAVMVYKIFKKLRMPLDQHVKYV
                     QKLVRYHLRPISLTKEDITDSAIRRLLFDAGDEIDDLMILCEADITSKNEKKVKRLLE
                     NYEMVRLKLKEIEDKDRIRNWQPPVSGELIMETFDLKSGPIVGELKNEIREAILEGDL
                     ENEYNAAYHFLLELGAKLGLERKSSAQ"
     gene            3955..4425
                     /locus_tag="PKV31_07760"
     CDS             3955..4425
                     /locus_tag="PKV31_07760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB92792.1"
                     /translation="MKILKKFVLIAPILLLIACEKDPSSVDDSAQLEIDKQKIRAYLN
                     QYNIKADSTSEGLYYVIDTKSDTSTRKPTSTSKVKVDYKGYLLNGKVFDNGTGVSFFL
                     TSLIPAWQIGLPKFNEGDKGRLFVPSKLGYGKSGSGSIPANSPLIFDITLIKVD"
BASE COUNT         1407 a          798 c         1070 g         1252 t
ORIGIN      
        1 atacattgcg ggaagagatc gaagagttgg accgaaaggc gcagaaggct gaacgagaga
       61 gtgactttga gactgttgcc aagattcgtt atggagaact acaggccaaa caacaggaat
      121 tgaaggagtc agaaaacgag ctgaatgcac tgcctgaagg ttcacgattc accgctcaaa
      181 tggttactgc taacgatatc gcggatgtcg tatctaaatg gaccggcata cctgtctcca
      241 aattacagca aagcgagaaa gacaaattgc tcaatatgga aaaggaaata gggaaaatgc
      301 tcattgggca gactgaagcc gttgaggcag taagtgaagc agtaagacgg aatcgtgcag
      361 gattgagcga cagtaagagg cctgtgggat cctttatttt catgggacct acaggggtag
      421 ggaagaccga gttgaccaaa gcgcttgcgg atgttttgtt cgatgacaag gatgctataa
      481 tcagaattga tatgagtgaa tacatggaaa aacactcggt atcaagattg gtaggcgcac
      541 ctcccggtta tgtaggttat gaagagggtg gtcagttgac agaggcagtg cgtcatagac
      601 catactccat cgtattgctg gatgaaatag aaaaagccaa tgcggatgtg ttcaacattc
      661 tcctgcaggt acttgacgat ggacggttga cagatggaaa gggaagggag gtcaatttca
      721 agaataccat tatcatcatg acatccaacc ttggttctaa cattattctt gagaattttg
      781 aggatttgga ggagattgag cctaaaaggc gatctgaagt aatagaaact acgaaattgg
      841 aagtttttga attgctgaaa caaaccatgc gtcctgaatt tctcaatagg attgacgaaa
      901 ccatcatgtt cttgcctttg accaaagaag agataggaaa gattctcgat attcagatga
      961 aaggcatcaa caaattgctt aagtctcagg aacttaggat tgaattggcc ccaagtgcct
     1021 ctaaactgct gattgatctg ggttatgacc ctcagtttgg agcaagacct ctgaaaaggg
     1081 tcctgcaaaa agagctggtc aatgaattgt caaaacaatt attgagtgga gattatttgc
     1141 cgggcgatgt tattgttgca gaaggtaaca ataccggatt gacatttcat aagaaaaagg
     1201 aagttatgac tgagcctgtt actgaaaatc ataccgacca tactgattca ggtagatctg
     1261 aagaaaaata agctatggct tatttgcatt gagttggaaa aatgcctaca acctattcag
     1321 ggcaatcact ttcgggtggt tgcctttttc tctttcatta cgttcagtct tgttaagtat
     1381 taaggaatta aagacaagat cccaacaatt accttatttc ttgtattttt gtcctttcaa
     1441 ttaatgattg aatggttaaa atcggtggta tttcattagg tgaatttccc ttgcttcttg
     1501 ccccaatgga agatgtgagt gatcctcctt tcagagcact ctgcaaagcc cagggatgcg
     1561 atatgctata cactgaattc gtttcagtag aaggtttgat tcgaaatgcc gataaaagtg
     1621 tgcagaaact ggatatttct gacgaagaac gaccggtggg tattcaaatc tttggatctg
     1681 aaaaagaatc aatgattcag gctgctgaaa tcattgaacg gactcaaccg gaggtgcttg
     1741 atatcaatta cggatgtccg gtgaaaaagg tcgtttgcaa gatggccggt gcaggtatac
     1801 ttcaggatat cgatcgtatg gtctctctca ctgaagcagt agtaaaagcg acctctttgc
     1861 ccgttaccgt taaaacaaga ctgggttggg atgaagattc aaagaatatt gtcgaagtag
     1921 ccgagaggct tcaggatata ggcatcaagg cattgtctat tcacggacgg actaggaagc
     1981 agatgtatac gggagaggcg gactggaccc taattgccaa agtgaaggaa aatccacgca
     2041 tacatattcc tgttttcggt aacggagata ttgattctcc tgagaaagcc aaactgtaca
     2101 aagaacgtta tggcgtggat ggcatcatga tcggaagagc aagcataggt aatccctgga
     2161 tattcaggga gataaaacag tatctgaaag atggaactat acccgatcca ccatcactcg
     2221 acgagcgaat tgaggctgta cttcagcatc tccacagatc catcatgtgg aagggagaca
     2281 aggttgggat caatgaaatg aagcgtcatt atggtaatta tttcagagga tatcgcgagg
     2341 taaaacagtt taggacactg ttggtacatt cttcatcttt cgatgaagtg gtggaaatta
     2401 cggaaaagat gcgtgaatat tacgcaggag aagagttgac ggtttaaatt ttaaaacaat
     2461 gtttctactg ttaaacagaa gcgaaaacga agaatttcta tacaaaattt tagcccgtgc
     2521 ggcttcagat ctcgacctac agacctatct tgtaggaggt tatgttcggg atctgattct
     2581 gggacgcaat tctacagata ttgatgtagt ttgtgttgga gatggtattc agttggcaga
     2641 gcaggtcaat aagcttcttg gtaataagca gccaatagtt gtctataaga attttggaac
     2701 agctgcactg agatataatg atattcaaat tgaatttgtt ggagcccgga aagaatctta
     2761 ccagaaaaac agccggaaac ctgccgttat agcaggtact ttgtacgacg accaactgag
     2821 gcgggatttc accatcaatg ctttggctat aagtctcaat accgatacct atggtgagtt
     2881 gctcgatcca tttgacggta tggaacacct ggaagagaag ttgataaaga cgcctttgga
     2941 tccggaagac acattctctg atgacccgct tagaatgatg agagcgattc gtttttccaa
     3001 tcagttgaat ttctccatcg acccgctcac ctatgctgca atcaatcata ataaggatag
     3061 attgaaaata gtcagcatag aacgaattgt tacagaattc caaaagatca tggcctgtga
     3121 taagccatcg atcggcatta agttattgtt cgataccggt ttgcttgaag tcttcctgcc
     3181 tgagctggtt gaattgcaag gagtggaata ccaggatggc aaagggcata aggataactt
     3241 ttatcatacg ctggaggtgt tggacaatgt ttgtcgcaat tcggataata tttggttgcg
     3301 ttgggctgcc ctccttcatg atattgccaa gccggctacc aagcgttttg atgaaaagca
     3361 aggatggacc ttccatggtc acgatgcatt gggtgctgtg atggtatata aaatttttaa
     3421 aaaacttcgc atgccattgg atcagcatgt caaatatgtg cagaaactgg tacgatatca
     3481 tttaagacct attagcctaa ccaaggagga tataacggat agtgccatca gaaggctatt
     3541 gttcgatgcg ggggatgaga ttgatgacct catgattctt tgtgaggcag atataacctc
     3601 caaaaatgag aaaaaagtga aacgattgct tgaaaactat gagatggtca ggcttaagtt
     3661 aaaagaaatc gaagacaaag acagaattag gaattggcag ccccctgtgt ccggtgaact
     3721 tatcatggaa actttcgatt tgaaatcggg cccgatcgta ggtgagttga aaaatgagat
     3781 ccgtgaagct attcttgagg gggatttgga aaatgaatac aatgctgctt atcatttttt
     3841 actggagctt ggagcaaagc ttggcttgga aagaaaatct tccgcccaat aaacgaatac
     3901 ctttctattc tcgtctttta tttgaattgt agttagacat aaaatccttt tttaatgaaa
     3961 atacttaaga aatttgtttt aatcgcaccg atcctattgc tgatcgcttg cgaaaaagat
     4021 ccatcatcag tagatgattc tgcccaattg gagattgata agcaaaaaat cagggcttac
     4081 ctcaatcaat ataacatcaa ggctgattct acctctgaag gcctttatta cgttatagac
     4141 accaaatcgg atacttctac caggaaacca acttcaacat caaaggtcaa ggtggattat
     4201 aaaggctatc ttttgaatgg aaaggttttt gacaacggaa caggtgtctc tttcttcctt
     4261 accagtttga ttccggcatg gcagatcggc ttaccaaaat tcaatgaagg agataaaggt
     4321 cgcttgtttg taccttcaaa attggggtat ggaaagagtg gctcgggatc tattcctgca
     4381 aattcaccat taatttttga cattacgctg attaaggtgg attgagtaat tctttttcag
     4441 taataaattt atatagtaag catggtttaa tgcttaacaa aaaaccgaac aacaaaatgc
     4501 tgttcggttt ttctttatta ttgtttt
//
LOCUS       DAOQMC010000188         4489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9283_length_4489_cov_3.256202, whole genome shotgun sequence.
ACCESSION   DAOQMC010000188 DAOQMC010000000
VERSION     DAOQMC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9283_length_4489_cov_3.256202"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..2835
                     /gene="nuoG"
                     /locus_tag="PKV31_07765"
     CDS             184..2835
                     /gene="nuoG"
                     /locus_tag="PKV31_07765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNB92793.1"
                     /translation="MAKVYIDNVEYEVPDGKNLLEVCLSLGKDITYFCWHPKMHSVGA
                     CRQCAVTVYRDENDTRGRLGMACMMDAVDGTRISIQDESSKNFRAGNIESLMINHPHD
                     CAVCDEGGECHLQDMTVMSGHNYRNYRFEKRTHINQDLGPFINHEMNRCIQCYRCVRF
                     YNDYAGGEDFGVFASSGRVYFGRSEDGTLENEFSGNLVEVCPTGVFTDKTLRQHYTRK
                     WDLTSAPSICQGCSVGCNILTSERYGKVRRVMTRFNGDVNGYFLCDRGRFGYEYANDE
                     KRLTRAAQKDNSSGTNAFTSIIQHLGTKPGMIGIGSPRASLESNFMLKQLVGAENFYS
                     GISGREKEVVDAALDVLANGKVKTPTLAEIENYETVFVLGEDVTNTSPMIALALRQTS
                     KTLPRQKAYALRIQTWNDAAIREVVQDDKGPFYIATPGRTKLEEIATSATHGDTKTIL
                     DIGNEVAELIKSGKADASNESFAHQTASALLAHDKPLIVSGAGMMSSLIVRCAAQIAT
                     ALKEAGKDVGLVLSVPEANSIGLAMIGSAGSFEDAMQRVEEDRRLTAVVIETDLYYRT
                     DRQSASSFLNNCNKVILLDSLENDTTPEVDYVLPAGTFAESSGTLVNNEGRAQRFYQV
                     FQPGNDMKSSWAWLDMMIHHSETARPFDDIITEMAAAIPAFEGIKSLAPNADFRIGTQ
                     KIPRESHRYSGRTSMNASVSVHEPKPPVDVDSPLSYTMEGFHGKAPSSDIPFFWSPGW
                     NSVQSINKYQVEVGGPLHGGNPGLRLIEASEDGNASFESHEYQGKGTFEVLPAWHIFG
                     SGELTALSPAIAKRIPEATASISDAGLAKAGLTGSTNIRVRYNGREIVLPVNIDNSLP
                     ENVVMIPYGLKETAGVHYPFFGEIESV"
     gene            2873..3829
                     /gene="nuoH"
                     /locus_tag="PKV31_07770"
     CDS             2873..3829
                     /gene="nuoH"
                     /locus_tag="PKV31_07770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNB92794.1"
                     /translation="MNETLFHILVVVGVLLGTVTIAAALIYVERKALALWQQRHGPNR
                     VGPFGLFQVVADVIKIFFKEDWIPPFVNKKVFIIAPTIIMLSILMSFAVVPFGPGLVV
                     ADMNIGLLFFLAMSSLGAYTIILAGWASNNKYSLLGGVRGAAQMISYEVFMGLSLMGI
                     VIQAGSFNLTDIVNAQKDVWFVIPQFLGFVIFLIAGIAETHRLPFDLPEAESELIAGF
                     HSEYSGMKFGMFFVGEYLGITLVSCLITVLFFGGWLGPAFLPPIVWFLLKMFCFILFF
                     ILLRATLPRPRYDQLMSYGWKLLLPLALLNVLVTGAIVLFMQ"
     gene            3843..4358
                     /gene="nuoI"
                     /locus_tag="PKV31_07775"
     CDS             3843..4358
                     /gene="nuoI"
                     /locus_tag="PKV31_07775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNB92795.1"
                     /translation="MFSDIRSLWMVFLKMFKKKVTIQYPEQKPYLTPRWRGRIILSRD
                     PDHNERCVACNLCAAACPTDCISLQATEDEEGRRYPEYFRINFSRCIFCGLCEDACPT
                     YAIQLTPDFEMGEYYRNNLVYEKEDLLIDGMGKYHGYSYYQQAGVAVGVKPKGGGENE
                     SAPVDVRTLLP"
BASE COUNT         1242 a          848 c         1097 g         1302 t
ORIGIN      
        1 atatcgagca gcttgagaag caaacacggt atatggctcc gggtaacacg ttctgcgccc
       61 ttgcacccgg tgctatggaa ccacttcaaa gtgcactgaa atacttcagg gaagattttg
      121 aagagcacgt ccacaacaaa cattgtaaat atcatcattg attgatatca taaaataaga
      181 gatatggcta aagtttatat cgataacgta gaatacgaag ttcctgatgg taagaatttg
      241 ctggaggtgt gcttatcctt gggtaaggac atcacctatt tttgctggca cccgaagatg
      301 cattccgtag gtgcctgtcg tcagtgcgcc gtaaccgttt atcgcgatga aaacgatacc
      361 cgtgggagat tggggatggc atgtatgatg gatgctgtgg atggtacaag aatttccatt
      421 caggatgaaa gctcaaaaaa cttcagggcc ggtaatattg aatcattgat gattaatcac
      481 ccgcacgatt gtgcagtgtg cgatgaaggc ggtgaatgtc atcttcagga tatgacggta
      541 atgagcggtc acaactatcg caattaccgt tttgagaaaa ggactcatat caatcaggat
      601 ttagggccct ttatcaatca tgagatgaat cgctgcatcc aatgttaccg atgtgttcgt
      661 ttctataatg actatgctgg tggtgaggac ttcggtgttt ttgcgtccag cggtagagtt
      721 tattttgggc gcagcgagga tggaaccctc gaaaatgaat tcagtggaaa tctcgtggag
      781 gtatgcccta caggagtgtt taccgataaa acactacgtc agcattatac cagaaaatgg
      841 gatttgacca gtgccccatc catctgtcaa ggctgttcag taggatgcaa tatccttact
      901 tctgagcgtt atggcaaagt tcgtcgagtg atgacgcgct tcaatggaga tgtaaatggt
      961 tactttcttt gtgatcgtgg gagattcgga tatgaatatg ccaatgatga aaaacgtttg
     1021 acaagagctg cccaaaagga taattcttcc ggtaccaatg ccttcacatc aattatacaa
     1081 catttgggta caaagccggg tatgattggt attggatcgc ctcgagcttc ccttgaatcc
     1141 aatttcatgc tgaagcagct ggtaggagca gagaatttct attctggtat ttccggaaga
     1201 gagaaggaag ttgtggatgc tgctctggat gttcttgcca atggaaaagt aaagacgcct
     1261 actttggcag agattgaaaa ttacgaaacc gtatttgtat tgggagaaga tgttaccaat
     1321 acatctccaa tgatagcatt ggcattacgc cagacttcca agacccttcc acgccaaaag
     1381 gcctatgcac tcaggattca aacctggaat gacgcagcaa tccgggaagt agtacaggat
     1441 gacaaaggac cattctacat agccacgccc ggaagaacaa aacttgaaga aatagcaact
     1501 tcagccacac atggtgatac taagacaata ttggacattg gaaatgaggt tgctgagttg
     1561 attaagtccg gtaaggccga tgcgtcaaat gaaagctttg ctcaccaaac tgcttcagca
     1621 ttattagcgc atgacaagcc tttgatagta tcgggcgcgg gaatgatgag cagtttaatc
     1681 gtgcgctgtg ctgctcaaat cgcaacagct ctgaaagaag ccggtaagga tgtggggctt
     1741 gtcctgtctg tacctgaggc caactccatt ggattagcta tgatcggatc agccggaagc
     1801 tttgaagatg ccatgcaaag agtggaggaa gacaggagac tgacagcagt tgttatagaa
     1861 acagacttgt actataggac tgatcggcag tcagcttctt cattcctgaa taattgcaac
     1921 aaggtcattc tccttgattc tttggaaaat gatactacac cggaggtcga ttatgtctta
     1981 ccggcgggaa cctttgcaga gtcaagtggg acattggtca ataatgaagg tcgtgcgcag
     2041 cgtttttatc aggttttcca gcctggtaat gatatgaaga gcagttgggc atggcttgat
     2101 atgatgattc atcattcgga aacagcaaga ccgtttgacg acatcatcac agaaatggct
     2161 gctgctatac ctgccttcga aggaatcaaa tcgctggcac ctaatgctga ttttagaata
     2221 ggaacgcaga aaattcctcg tgaatcacat agatatagtg gaaggacatc catgaatgct
     2281 tctgtaagtg tacatgagcc caagccccct gttgatgtgg actctccatt gagctatacg
     2341 atggaaggat tccatggtaa ggcaccttcc tctgatattc cgtttttctg gtctcccggc
     2401 tggaattctg tacaatctat caacaaatat caggtggaag ttggtgggcc cttacatggt
     2461 ggtaatccgg gattgaggtt gattgaggcg tctgaggatg gaaatgcttc gtttgaaagt
     2521 catgaatatc aaggcaaagg tacctttgag gtattaccgg catggcatat tttcggatcc
     2581 ggtgagttga cagcattgag ccctgccata gccaaacgga taccagaagc aaccgcgtcc
     2641 atttcagatg caggattggc aaaagccggt ctcacagggt caacaaatat aagagtgcga
     2701 tacaatggta gagaaattgt tttgccggtg aatattgata atagtcttcc ggaaaatgtg
     2761 gtaatgatac cttatggact gaaggaaaca gcaggtgtcc attatccgtt ttttggagaa
     2821 attgaaagcg tgtaataaaa agggataagt taaaatttaa taatcaaggg atatgaacga
     2881 aaccctgttt cacatactgg ttgtagtcgg cgtactgttg ggtacggtga ctatcgctgc
     2941 tgctttgata tatgtcgaaa ggaaggcgct tgcattatgg caacaacgcc atggccctaa
     3001 tagagtgggg ccatttggac tcttccaggt cgttgctgac gtaattaaga tcttcttcaa
     3061 agaagactgg attccacctt ttgtcaacaa aaaggttttc atcattgcac caaccattat
     3121 tatgcttagt atattaatga gttttgctgt agttccattt ggtccgggtt tggttgtggc
     3181 agatatgaat ataggccttt tgtttttcct ggcaatgtca tctcttggtg cttacacaat
     3241 catacttgca ggttgggctt ccaacaacaa atactctctg ttgggaggcg tacgcggagc
     3301 agcacaaatg atttcatatg aggtgtttat gggtttatca ctcatgggaa ttgtaattca
     3361 ggcaggttca ttcaatttga ccgatatagt caatgcgcag aaggatgtat ggtttgtcat
     3421 tcctcagttt cttggctttg tgatcttttt gatagcggga attgcagaga cacaccgatt
     3481 gcccttcgac cttcctgaag cagaaagtga gttgattgcc ggcttccact ctgaatattc
     3541 aggaatgaag tttggaatgt ttttcgtagg tgaatacctg ggcattacac ttgtgtcctg
     3601 cctcattacc gttttgttct ttggtggatg gctagggccg gcgtttttgc ctccaattgt
     3661 ttggtttcta ttaaaaatgt tttgtttcat attgttcttt atcctattgc gtgctacttt
     3721 acccagaccg cgatatgatc agttgatgag ctacggctgg aagctgttat tgcctcttgc
     3781 tttgctcaat gtactggtga ccggagcaat tgtacttttc atgcaataaa tatttaagaa
     3841 tcatgtttag tgatattcgt tctttatgga tggtctttct gaaaatgttc aagaaaaagg
     3901 ttactataca gtatcctgaa cagaagccct accttacacc acggtggcga ggtcggatca
     3961 ttctttcgcg tgatccggat cataatgaaa gatgtgtagc atgtaacttg tgcgctgctg
     4021 catgtccgac agattgtatt tccttacagg ctactgaaga tgaggaagga agaagatatc
     4081 cggaatattt caggattaat ttttcaaggt gcatattttg tggactttgt gaagatgctt
     4141 gtccgaccta tgcaatacaa ttgacaccgg attttgagat gggtgaatac tatcgtaata
     4201 atctggtcta tgagaaggag gatttgttga ttgatggaat gggtaaatat catggttaca
     4261 gttattatca acaggcagga gtggcagtag gcgtcaaacc aaaaggcggc ggagaaaatg
     4321 aaagtgctcc tgtcgatgta aggactttat taccataaaa ctattaacta gcttttcatg
     4381 aatataattt tctatatagc atcaattgtt gccttgatcg ccaccatcag ggttgttaca
     4441 cacaaagacg cagtgcacgc tttgttgtat ctggttgttt ctttattgg
//
LOCUS       DAOQMC010000189         4476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9329_length_4476_cov_5.186157, whole genome shotgun sequence.
ACCESSION   DAOQMC010000189 DAOQMC010000000
VERSION     DAOQMC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9329_length_4476_cov_5.186157"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="selD"
                     /locus_tag="PKV31_07780"
     CDS             complement(<1..461)
                     /gene="selD"
                     /locus_tag="PKV31_07780"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNB92796.1"
                     /translation="MTEEKIRLTKYSHGGGCGCKIAPQVLDEILKGSVSGITSGKLIV
                     GNGSRDDAAVYRLNDNEALISTTDFFTPIVDDPFIFGKVAAANAISDVYAMGGDPIMA
                     LAILGWPIDKLPVEAARAVLDGAITICNEAGIPLAGGHSIDTPEPIFGLAV"
     gene            complement(458..1498)
                     /gene="mnmH"
                     /locus_tag="PKV31_07785"
     CDS             complement(458..1498)
                     /gene="mnmH"
                     /locus_tag="PKV31_07785"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNB92797.1"
                     /translation="MAVQQIDIEQFLQLAEEHPVFDVRSPSEYGHAHIPGALPLPLFS
                     DDERREVGTTYKQRSREAAIKIGLDYFGIKMRQMVCLVEDILHHKGTNTVLLHCWRGG
                     MRSGAVSWLLDMYGFRVFSLRGGYKKYRNWVLDQFSRPYSLRILSGFTGSGKTEILLE
                     MARLGVPTVDLEGLAGHRGSAFGALGMPPQPTVEQFENKLAQELYTISREHPGKTIWL
                     ESESNRIGCVNIFHTFFNQMKAAPRIHTEVSHEARLGYITAGYGQFSTEKLKDAVLRI
                     QKRLGGLETKNALAHLDAGCIRDAFDILLKYYDKFYASGGIYQPAIMTIELPGTNAYE
                     NARLILQKTKDL"
     gene            complement(1968..3314)
                     /locus_tag="PKV31_07790"
     CDS             complement(1968..3314)
                     /locus_tag="PKV31_07790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB92798.1"
                     /translation="MTRIFSVLALFMPIMCLAQPKVLNKVIAQVGGETVLLNEVEDNY
                     LGQTRGMTNVPPDLKCQIFEGLLLQKLLTNQAKIDSVKVSDEQVENQLNARFDRVLAY
                     MNNDMAQFEEYYGKSVNEMKDELREDLKDQMMAEEMKNKVVDKITITPSEVKAYFKSI
                     PHDSLPYYNSEVEYSELVYYPKPNKLERQRVIDKLNSIRTRIVENGEKFEDLAKKFSM
                     DGSAAAGGDLGWAKRGAFVPEFEAVVFNLEEGQVSTIVETEFGFHLIQLLERRGNSFH
                     ARHILIKPELSSSDKEAALKMLDSIVLQIRTGKLSFPDAVKKFSNKNYPSYSNGGRAI
                     NPHTNTNFFEISDLEPDVYFALDTMKIGGMSAPIELKDPYGETAYQVLLLQARTDPHR
                     ASLEKDFSRIKESAMMYKKEAELDNWVTTKIRDTFITIDPEWLGKCPGVQKWVKAN"
     gene            complement(3324..>4476)
                     /locus_tag="PKV31_07795"
     CDS             complement(3324..>4476)
                     /locus_tag="PKV31_07795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB92799.1"
                     /translation="IARKYSGDLASAPKGGFLGFITIGQYDNAFEDPVFALTKDGEYT
                     KPFKTALGYHIVKRISKKDMSDFNSVKKSLMTKVSQNERFGIAKHKLVEDIKKEYHFT
                     QDNKVLGQYIDSVNTDRFYSNIWTAPGIPKATIFSIGENKYTTDDLSKYLVDNTRRRV
                     RMNKSVLPSIAFNELFDEFVSNKAIEYEQSRLEYKYPEYKALTREYEEGFLFFEVTNN
                     EIWNKASQDSVGIEKYFNEHRDKYMWEERARVLHYSIKASGPEELNRIFELARKYTPE
                     ELQKKYSLDKVSYTSETLEKSSASDMKGLKWKQGEMSELHRNPDGRTGTFDKVESIMQ
                     PVRKELKDARGFVIADYQDFLDKQWVEKLKQEYSVTVNKNVFNSLIKHK"
BASE COUNT         1159 a         1097 c          913 g         1307 t
ORIGIN      
        1 ctaacggcaa gcccaaatat cggctcagga gtatctatgc tgtgtccgcc ggccaacggt
       61 attccggctt cattgcagat cgtgatagcg ccatcgagga ctgcacgggc agcttccact
      121 ggcagcttat caatcggcca gcccaggatt gccagtgcca tgatggggtc tccccccatt
      181 gcataaacat cactgatggc attggcagcc gcaactttcc cgaagataaa agggtcatca
      241 acaattgggg taaagaagtc ggttgttgaa ataagtgctt cgttgtcgtt gagcctatat
      301 actgccgcat cgtccctgga gccattgcca acgataagct tacctgaagt gatgccactg
      361 acactgccct tcagtatttc atccagcact tgtggagcga tcttacaacc acagcctcca
      421 ccatgggaat atttggtcaa acgaatcttt tcttcagtca taaatccttt gttttttgga
      481 ggatgagtct ggcattttca taagcattgg taccgggcaa ttcgatggtc atgatagccg
      541 gttgataaat tccaccggaa gcatagaatt tatcataata cttcagcagg atgtcaaagg
      601 catcacggat acaaccggca tcaagatgag ccaatgcatt ctttgtttca agcccgccca
      661 gccgcttttg aatcctcaaa acagcatcct tcagtttttc tgtactaaat tggccatagc
      721 ctgcagtaat ataacccagg cgtgcctcat gggacacctc ggtgtgaatc cttggtgccg
      781 ccttcatttg attgaaaaaa gtatgaaaga tattaacaca gccaatcctg ttgctctcac
      841 tttcaagcca aatcgtcttg ccggggtgtt ccctactaat cgtatacaac tcctgtgcaa
      901 gcttgttttc aaattgctcc accgttggtt gaggcggcat gcccaaagcg ccaaatgccg
      961 atcctcgatg ccctgcaaga ccttcaagat ccaccgtcgg tacacccagc cgagccattt
     1021 ccagaagaat ttccgtcttg ccgcttccgg taaaaccact caatatccta agtgaataag
     1081 gcctggaaaa ctggtccaat acccaattcc tgtatttttt gtagcccccc ctgagcgaaa
     1141 agactctaaa tccatacatg tcgaggagcc agctcaccgc accactgcgc atgcctccgc
     1201 gccaacagtg caaaagaact gtattggtac ccttatgatg caggatatcc tccaccaggc
     1261 agaccatctg cctcatcttg ataccaaaat aatccaggcc gatcttgatg gctgcttccc
     1321 gacttctttg cttataagtc gttcccacct ccctcctttc atcatctgaa aagagcggca
     1381 gtggcagtgc tccgggtata tgcgcatgtc catactccga aggactccgc acatcgaaaa
     1441 ccggatgttc ttcggccagt tgcaggaatt gctcaatatc tatttgctga actgccataa
     1501 tgcaaagcta ttaattttca aaaacaatca ggcaaacaat ggtgtaggat cacgccaata
     1561 ccatctatag aaaaacagtt tctcctgcaa tggccgggat gttccattgc atgtgttgat
     1621 acaccaacct gagaggtctg ccaaggtcaa aaatgtggca gatgacaagt gctaccgaag
     1681 taggtagatg attaatagat tgcgaaattg atttccctat atgatcgggc gattggtatg
     1741 aaaatccttg cccatttgtg aagtaaaagg taagggcccc gaccggagac tcctggctga
     1801 acagtaataa tgattattat tttcctactc acttcaggtt gacaaggcaa acaccctatc
     1861 aatcagcatt tctgtcccat tcccggactg ccttaatatt tatttggcaa agtaaaagca
     1921 gcctcaatac tcacttttaa tggattgatt gatccaaaga ctgaatgtca attggccttt
     1981 acccattttt gtactcccgg gcacttgccc aaccattccg ggtcaatggt gataaaggta
     2041 tcccgaatct tggttgtcac ccaattgtca agctctgctt cttttttata catcatggca
     2101 gattccttga tccgcgaaaa gtctttttca aggcttgctc tatggggatc ggtacgtgcc
     2161 tgaagcaaaa gtacctgata agctgtctcc ccatatggat cttttaattc aatcggtgcg
     2221 gacatgccac caatcttcat ggtatccaat gcgaaatata catcaggctc aagatcagaa
     2281 atttcaaaaa agttggtgtt agtgtgtgga ttaatcgccc gacctccatt gctgtaactc
     2341 ggatagtttt tgttgctgaa tttttttaca gcatcaggaa aactaagctt cccggtcctg
     2401 atctgtaata cgatactatc gagcatcttg agggcagcct ccttgtcgga agatgataat
     2461 tccggcttga taagtatatg acgagcatgg aaggaattcc cgcgtctttc caatagttgg
     2521 atgaggtgaa acccgaattc tgtctcaacg attgtactca cctgcccttc ttccaggttg
     2581 aagacaacag cttcaaattc tggtacaaag gcaccacgct tggcccagcc caagtctccc
     2641 cctgcagcag cagaaccgtc catggaaaat ttctttgcca ggtcttcaaa cttttcgcca
     2701 ttctccacga ttcgcgtgcg gatactgtta agcttatcga taacacgttg gcgttccagt
     2761 ttatttggct tagggtaata gaccaattcg ctgtattcga cctccgagtt gtagtaggga
     2821 agagagtcat gcggaatgga tttgaaatat gccttgactt cggatggagt aatggttatc
     2881 ttgtccacca ccttgttttt catctcttct gccatcattt gatctttcag atcttcacgc
     2941 agttcgtcct tcatctcatt gaccgacttg ccataatatt cctcaaattg agccatatcg
     3001 ttgttcatat aggcaagtac tctgtcaaaa cgggcattta gctgattttc aacttgctcg
     3061 tcactgacct ttactgagtc aatctttgct tgattggtga ggagtttttg caacaaaaga
     3121 ccttcaaaaa tctgacattt cagatcaggg ggaacattag tcatgcccct ggtctggcca
     3181 aggtaattgt cctctacttc attgagcagc acagtctcac cacccacctg ggctatgact
     3241 ttgttaagta ccttaggttg agcaaggcac attatgggca taaatagggc taaaacacta
     3301 aaaatacgcg tcattccgat cagttattta tgtttgataa gtgagttgaa aacgttttta
     3361 ttgacggtaa cgctgtattc ctgcttaagt ttttcaaccc actgcttgtc cagaaaatcc
     3421 tgatagtcag cgataacaaa acctcgcgca tccttcagtt cctttctgac cggttgcatt
     3481 atgctctcca ctttgtcaaa ggtaccagtc ctgccatcag gatttcgatg caactctgac
     3541 atttcaccct gtttccactt caggcctttc atgtcgctgg cacttgactt ctccagagtc
     3601 tcagatgtat aggaaacctt atccaggcta tattttttct gcaattcctc aggtgtatat
     3661 ttcctggcca attcaaagat gcggttcagc tcttccggtc cggaagcctt aatactataa
     3721 tgcaggactc tggctcgttc ttcccacatg tatttgtccc gatgctcatt gaagtacttt
     3781 tcaataccta cactgtcttg tgacgccttg ttccagatct cgttattggt aacttcaaaa
     3841 aagaggaatc cttcttcata ctctctggta agagctttat attcaggata tttgtattcg
     3901 agtcggctct gttcgtattc aattgcctta ttggatacaa attcatcaaa caattcgttg
     3961 aaagctatag atggcaacac gcttttattc atcctgaccc ttcgccgagt gttatcgaca
     4021 agatacttac taagatcatc tgtggtatat ttattttctc ctatactgaa gatggtagcc
     4081 ttaggtatgc cgggagcagt ccaaatattg gaatagaagc gatccgtatt gaccgaatcg
     4141 atgtattgcc ccaaaacctt attgtcttga gtgaaatgat actccttctt gatatcctcc
     4201 accagcttgt gctttgcaat accgaatcgt tcattctgac tgaccttggt catcaatgat
     4261 ttcttcacag aattgaagtc ggacatatct tttttggaga tgcgcttaac gatatgataa
     4321 cccagtgctg tcttgaatgg cttggtgtat tctccgtctt ttgtcaaagc aaataccgga
     4381 tcctcaaaag cattgtcata ctgaccgata gtgatgaagc ccagaaagcc gcccttggga
     4441 gcagatgcca gatcgccgga atattttctg gcaatc
//
LOCUS       DAOQMC010000190         4461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9383_length_4461_cov_4.449387, whole genome shotgun sequence.
ACCESSION   DAOQMC010000190 DAOQMC010000000
VERSION     DAOQMC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9383_length_4461_cov_4.449387"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1489
                     /locus_tag="PKV31_07800"
     CDS             92..1489
                     /locus_tag="PKV31_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNB92800.1"
                     /translation="MLQKTIKDKVSIKGIGLHTGKTVEMTFIPAAENHGIKFQRIDTP
                     EQVIIPADILRVVSTNRGTAISSGEITIYTIEHALAALTGLGIDNVLIEINGPEVPIL
                     DGSAKPFVDAIEQTGIVEQNEEKDYFIITEPISYKDETTGSEMIALPGDDFEVTTMID
                     FASPVLGEQFAKLDHISQFKEEISACRTFVFLHEVEKLLNQGLIKGGDLDNAIVIVDR
                     MMNQQELDDLAKKLGKPSVKIEKEGVLNTLKLHFSNEPARHKLLDVIGDITLLGRPIK
                     GKIVATKPGHSVNVEFTKLLRKAANEQKKLKGKPIYDPDKDAVMDTTQIMGMLPHRHP
                     FLMVDKVIEINENYVVGVKNISFTEACFQGHFPGNPVFPGVLQIEAMAQTGGILCLSL
                     MPDPENWDTYFLKITNAKFKQKVVPGDTMVIKMELLEPIRRGICHMYGIAYVGNKIVS
                     EADLTAQIVNRTTIK"
     gene            1489..2262
                     /gene="lpxA"
                     /locus_tag="PKV31_07805"
     CDS             1489..2262
                     /gene="lpxA"
                     /locus_tag="PKV31_07805"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNB92801.1"
                     /translation="MTPKFSDIHPNAQIGIGTQINNFVTIEDDVQIGENCWIGSGAVI
                     MKGARLGNNVRIFPGAIIAGIPQDLKFQGEESLVEIGNNTTIREYVTVNRGTAAAGTT
                     IIGENCLIQAYCHVAHDCKIGNNCIMSNNTALAGHVVLEDYAIIGGMSAVHQFSRIGC
                     HTMIGGYSKVRTDVPPFIKAARDPLSYAGVNSIGMKRRGFTQDRVHHIQDIYRILFVH
                     GTNFHKSIEQIENNIPNTEDKTYILNFLKSSTRGLIKGI"
     gene            2278..2925
                     /locus_tag="PKV31_07810"
     CDS             2278..2925
                     /locus_tag="PKV31_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB92802.1"
                     /translation="MIPKVEVITCKEIGKRYSYEWVLRDFDFQFKKGECYAIRGPNGS
                     GKSTLLKILSGYSTPSKGKICYSSGNTDIPTSDIYRHIAFSAPYIELIDILTVDELLD
                     MHHGLRPFINMDTVRETIKNLPFKNMFRKKVEELSSGMKQRLKLILSIMSQSSVILLD
                     EPGSNLDDDGKQWFEELLKPRIKDHIVIIASNEESDLRLAQHLIKITDYKKAKQS"
     gene            complement(3003..3740)
                     /locus_tag="PKV31_07815"
     CDS             complement(3003..3740)
                     /locus_tag="PKV31_07815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB92803.1"
                     /translation="MSSSVLLFIDIETVSTVGEYLELNPAMQAFWRKKASGWIRQSDE
                     VNDDLYGNLFKDKAAIFAEFGKIICISIGYNTFDSTTKQAAFRVKSFYGHDEKQLLTD
                     FLNLVAKHFHDPKKHTFCGHNIKEFDIPYICRRAMIHQLHLPPTFQLNGKKPWEVNHL
                     VDTLEEWKFGDFKHYISLALMAELFGIPTPKDDIDGSMVGKVYWEDKDLERIRVYCQK
                     DVHTVARIYSKLTGGRYQIAEEIVEVE"
     gene            3912..>4461
                     /locus_tag="PKV31_07820"
     CDS             3912..>4461
                     /locus_tag="PKV31_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92804.1"
                     /translation="MEERGFNNTYKAISITIILLLLALSGYLLYDKSQSANTIANQEA
                     MIVEKEKSRADLEKTYYEALANLEQMKGDNEELNGRIDSLKTELKKQRDQIVSLMQTS
                     KDYQAIKGKIASMKAESEEYKKQIEALRSENETLSRANQQLNTEKEILTGEINKERMT
                     NDEISARNASLETEKANIETERA"
BASE COUNT         1517 a          937 c          873 g         1134 t
ORIGIN      
        1 ctgacgcttg agataaactt tattcaattt catattatat tcttactttt gccaaaattt
       61 ataaatttaa tttctggctt aacaacattt tatgttgcaa aaaacaataa aggataaggt
      121 tagcataaaa ggaatcggcc tccataccgg caaaaccgtt gaaatgacct ttattccggc
      181 tgctgaaaac cacggtataa aatttcaacg catcgataca cccgaacagg ttattattcc
      241 ggctgatatt ttaagagtag tatcaaccaa tcggggaaca gccatcagct caggcgaaat
      301 caccatctac acaattgagc atgcattggc ggcacttaca ggccttggaa tagataacgt
      361 gcttatcgaa ataaatggtc ctgaagtacc aatcctcgat ggaagtgcaa aaccatttgt
      421 cgatgcaata gaacaaacag gcatcgtaga acaaaacgaa gaaaaagatt attttatcat
      481 aacagaaccc atctcctaca aggatgagac tacaggaagt gaaatgatag ccttaccggg
      541 cgatgatttc gaggtgacta ctatgattga ttttgcctca cctgttttgg gagaacaatt
      601 tgccaaactg gaccatatta gccagttcaa ggaggagatt tcagcctgtc gtaccttcgt
      661 atttctgcat gaagtagaga agttgctcaa tcaagggctg atcaaaggag gcgacctgga
      721 taacgccata gttatagtgg atagaatgat gaaccagcag gaactggatg acttagcgaa
      781 aaaactgggc aaaccaagcg tcaagataga aaaggaaggc gttcttaata cgctcaagct
      841 gcatttcagt aacgaaccgg caaggcataa actattggat gtaattggtg acataacctt
      901 gctgggaagg ccgatcaaag gaaaaatagt tgctaccaaa ccgggacatt ctgtcaacgt
      961 ggaatttacc aaacttctga ggaaggctgc caacgagcag aaaaagttga aaggtaagcc
     1021 aatctatgat ccagacaaag atgctgtgat ggacaccacc cagatcatgg gtatgctgcc
     1081 tcataggcat ccattcctta tggtggacaa agtaatcgaa ataaacgaaa actatgtagt
     1141 cggtgtaaag aacatatcgt ttactgaagc atgttttcaa ggacatttcc ccggcaatcc
     1201 ggtatttccc ggtgtattac aaatcgaagc aatggctcag accggtggaa ttctttgtct
     1261 ttctctgatg cccgatccgg aaaattggga tacctatttt ctaaaaataa ccaacgccaa
     1321 attcaaacaa aaggttgtcc ccggagatac aatggttatc aaaatggaac tcctggaacc
     1381 tatacgacgt ggaatctgcc atatgtacgg tattgcctat gtaggcaaca aaattgtatc
     1441 agaagccgat ctcaccgctc aaattgttaa caggacaacc attaaataat gacaccgaaa
     1501 ttttcagata tccatcccaa tgctcaaata ggaatcggca ctcagatcaa caacttcgta
     1561 accatagaag acgatgtaca aatcggcgaa aattgttgga tcggatcagg tgccgtaatc
     1621 atgaagggcg ccaggctagg caataatgtc cgtatatttc cgggtgccat cattgccgga
     1681 ataccgcaag acctgaagtt tcaaggagag gagtctcttg tcgaaatcgg aaacaataca
     1741 acaatcaggg aatatgtcac ggtcaaccgg ggtacagcag cagcaggaac aaccatcatt
     1801 ggcgaaaact gtctgattca ggcctattgc catgttgccc atgattgcaa aattggcaac
     1861 aactgcatca tgtccaacaa tacagccctt gcaggccatg ttgtactcga agactatgca
     1921 ataataggag gcatgtcagc tgtacaccaa ttttcacgta taggttgcca taccatgatt
     1981 ggaggatata gcaaggttag aacagatgtc ccccccttta tcaaagcagc cagggacccg
     2041 ctttcctatg caggagtcaa ctctatcggt atgaaacgca gaggatttac ccaggaccgg
     2101 gtacatcata tccaggatat ttacaggatc ctgtttgttc atgggaccaa tttccataaa
     2161 tccatcgaac aaatcgaaaa caatattccg aatacagaag acaagactta tattctcaac
     2221 ttcctcaaaa gcagtaccag aggattgatc aagggtattt aatcaaaaat acctcttatg
     2281 attccaaagg ttgaagttat cacttgcaag gagattggaa aaagatattc atacgaatgg
     2341 gtattaaggg actttgattt tcaatttaaa aaaggagaat gctacgctat ccgtggcccg
     2401 aatggcagcg gaaaatccac cctgctaaag atcctcagcg gttactcgac cccttcaaaa
     2461 ggaaagatat gttattcaag tggaaatacc gacataccta caagtgatat ttaccgacat
     2521 atcgcatttt cagcacccta tattgagctg attgatattc tgactgttga tgaactgttg
     2581 gatatgcacc atggtctaag accatttatt aatatggata ctgtccgtga aactatcaaa
     2641 aatctacctt tcaaaaatat gttccgaaag aaagttgaag agctctcgtc aggaatgaaa
     2701 cagcgattga aactcatcct ttccattatg tctcaatcct ctgtaatcct gctggacgaa
     2761 ccggggagca acctggatga cgacgggaaa caatggtttg aagaactgct gaagccaaga
     2821 atcaaggacc acatcgtaat cattgcatct aatgaagagt ccgatctgcg tctggcccaa
     2881 catctgatta aaattacaga ctataagaaa gcaaaacaat cataggatca cctaaggtca
     2941 aaacatttcg aaataatcag gtaacaccgc cgcattttta aaaacctcca aataacaaac
     3001 ccctactcta cttctacaat ctcctcagcg atctgatatc ttccccctgt gagtttacta
     3061 tatattcttg caaccgtatg gacatccttt tggcaataca cacgaatcct ttccagatct
     3121 ttatcctccc aatatacttt tcccaccatg gaaccatcga tatcatcctt aggtgtagga
     3181 ataccgaaca actcagccat taaggcgaga gaaatgtagt gcttgaaatc tccaaacttc
     3241 cattcttcaa gggtatcaac aagatgattg acctcccagg gctttttccc attcagctgg
     3301 aaggttggcg gaagatgtaa ctgatgaatc attgctctcc tacatatata cgggatatca
     3361 aattccttga tattgtgccc acaaaaggta tgtttctttg gatcgtgaaa atgcttcgct
     3421 accagattca aaaaatccgt caacaactgt ttctcatcgt gcccataaaa ggatttaact
     3481 ctgaaagcgg cttgttttgt ggttgaatca aaagtgttgt agccgattga aatacagatt
     3541 attttcccga attcagcaaa aattgctgct ttatccttga acaaattacc atataggtca
     3601 tcattgactt catcggattg tctgatccac ccggatgcct ttttccgcca aaatgcctgc
     3661 atggcagggt tcaattcaag atactctcct actgtggaaa ctgtctcgat atcgataaac
     3721 aacaaaacag atgagctcat aacaacctta attatattaa attaatgaag aaaggagaaa
     3781 aaatactgac ttcccgccat ccaaataata gtggtatact attaatttta tcaattaaaa
     3841 atacaataca tttgcatttt gaaacttatt tatatcaaaa aagattttca tattaattta
     3901 aatctatcaa aatggaagaa agaggtttta acaatactta caaagctata agtattacaa
     3961 ttatattgct attattggct ttgagtggct acctgctgta tgacaaaagc caaagcgcca
     4021 ataccattgc caatcaggaa gcaatgattg ttgaaaagga gaaaagcaga gcagacctgg
     4081 agaaaaccta ttatgaagct ctggccaacc ttgagcaaat gaagggcgac aatgaagagt
     4141 taaacggcag gatagatagc ctcaagacag agttgaaaaa acagcgggac caaattgtct
     4201 ccctgatgca aaccagcaaa gactatcagg caattaaagg aaaaattgct tccatgaagg
     4261 ccgaatccga agaatacaaa aaacaaattg aagccctcag aagtgaaaat gaaaccttat
     4321 ccagggccaa tcaacagctt aataccgaaa aggaaattct cactggtgaa atcaacaaag
     4381 agagaatgac aaatgatgaa atttcggccc gcaatgcctc cctcgaaacg gaaaaagcca
     4441 atattgaaac cgaaagggca a
//
LOCUS       DAOQMC010000191         4456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9393_length_4456_cov_5.391956, whole genome shotgun sequence.
ACCESSION   DAOQMC010000191 DAOQMC010000000
VERSION     DAOQMC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9393_length_4456_cov_5.391956"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PKV31_07825"
     CDS             <1..1086
                     /locus_tag="PKV31_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92805.1"
                     /translation="IDYVLAHNIIYGSYEYGLENGIPQHKDFDKVTKYILEEDDERIP
                     LIELEFGRDGKPFYFQTESQSDAFAENIIRLLKKNVGEGNFYYTLLKDIEEESEEEDE
                     EVDDEEDPNNFNLPILKYLLNDGTVDIDKAKHTFNTLLDDGEMFAQYPLFISASMAII
                     HHYADEQRTNQLFEKWELDTTIDPEYEIEAGMSEQQIDRTYEILTEGKPSLKDIRYIR
                     KNISSEVLADYILMKASLPKIDHENEDDSDLDYSEYNKREEQFLKDHPDYPLSKALIA
                     SETSSPDDLTIESIFGSADNISVFEFNEYLMMKLETLKNKPDASAFLAFRAILGQYLE
                     SDTVHEEIFFTYELLTIQYIGDYLMDN"
     gene            1197..3500
                     /locus_tag="PKV31_07830"
     CDS             1197..3500
                     /locus_tag="PKV31_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB92806.1"
                     /translation="MNFSPEQGSLLAYEHYGITATARPLNGYDEQNFLLTAQDGNKYT
                     FKIAGSDISRTFLEAQADVMHHLDHSSIQGGFPVCLENKQGKAITTITYEGKEYHIRI
                     LTYLEGHFWVDEKNKNNFLYESLGEFLGAMDRSLEGICPDATVRNYVWDNNRAPEAEK
                     KLHFIRDHEKRRIAAYFLLQFRTEVTPVLSELRHACIHGDANDYNLLVRNGRVSGLID
                     FGDMIYSATINNLAIACAYALLHTEDPIEKASLIVKGYHSQYPLTEQEIDILYYLIAG
                     RLCISVTQSAEKSYSNSENEHHFLSEQSAWRLLYQLIRTNPLYAADQFRRSCGMMPCL
                     NPEDDYSSLLSERNRYIGRNLSISYRTPLKIVKGALQYLYDDKGKTYLDCVNNVSHVG
                     HCHPGVVYAIQSQAAILNTNTRYLHDNITSYAKALVDTLPERLSVCYFVNSGSEANDL
                     AIRMARHFTGHNDIVVLDHAYHGTSTLAMEISPYKFDGIGGFGARHYIHKAASPNLYR
                     GPYGIDDEAAGEKYAQSVKEIVQGLEREDKSPAAFICETLLGVGGQIPLPTGYLKQVY
                     QYIRSAGGVCIADEVQVGFGRIGEAFWGFELQEVTPDIVVLGKPIGNGHPLAAVVVTQ
                     AIADAFNNGMEYFNTFGGNPVSMAAGMAVLNIIRQEELQQHALNTGNGLMDDLRKLME
                     KHTIIGDVRGHGLFVGVELVRNRVSKEPAVPEIDVIVERMKGRGFLLSTDGPLHNVLK
                     IKPPMVISRQNCVDLVQALDEVLSEEL"
     gene            complement(3495..>4456)
                     /locus_tag="PKV31_07835"
     CDS             complement(3495..>4456)
                     /locus_tag="PKV31_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB92807.1"
                     /translation="LVGGRLDNDVVIEPKYLGNRKLLELCIATLLTDRGLLLMGLPGT
                     AKSWLSEHLAAAISGISTSMIQGTVGMDENQLKYHWNYAELLQHGPSERALVKSPVLR
                     AMEDGYITRIEELTRMPTEVQDILITVLSEKMMSIPELNMEVYAQKGFNVIATANDRD
                     KGVHDLSAALRRRFNIVYLSMPETLQEEIDIIHFRLGQIQRDLNLEKYKVPLQHLSDL
                     ITIFRELRNGETEEKKSKIKSPGSSLSTAEVLSVLQTGIQHSIHFGTGNLEPSDVASS
                     IISTVIKDPYQDKAAWDEYLESVIKNRKGWKPWYEAFSSASES"
BASE COUNT         1306 a          961 c         1003 g         1186 t
ORIGIN      
        1 atagattatg tcctggctca taacataatc tacggaagct atgagtatgg actcgaaaac
       61 ggaataccgc agcacaagga ttttgataaa gtgacaaaat atatcctgga ggaagacgat
      121 gaacgcattc ctttgataga actggaattt ggccgggacg gaaaaccatt ttactttcag
      181 acagaatctc aaagtgacgc atttgcagaa aatattatcc ggttattgaa aaaaaatgtc
      241 ggagagggca atttctatta tactttacta aaagatatag aagaggagtc ggaagaggaa
      301 gatgaagagg ttgatgatga agaagaccca aacaacttca acttgccgat cttgaaatat
      361 ttactgaatg atggtactgt agatattgat aaagcaaagc acacatttaa tactttactg
      421 gatgatggtg aaatgtttgc tcaatatccc ctgtttattt cagcttctat ggcaatcatc
      481 catcactacg cggacgaaca acggaccaac caactttttg aaaagtggga acttgatacg
      541 actattgatc cggaatatga aatcgaggca ggaatgtcag agcagcaaat tgacagaaca
      601 tacgaaattc taaccgaagg caagccatct ttgaaagata tccgctatat caggaagaat
      661 ataagtagtg aggtattggc cgattatata ctgatgaagg cttccttgcc taaaattgat
      721 catgaaaacg aagatgattc tgatttagat tattccgaat ataacaagag agaagagcag
      781 tttttaaaag accatcctga ttatccacta agtaaagcgt tgatcgcaag tgaaacatcc
      841 tcgcctgacg atcttaccat tgaatcaata tttggcagtg ctgataatat ttccgttttt
      901 gagttcaatg aatatctgat gatgaagttg gaaacattaa agaacaaacc ggatgcatca
      961 gcgtttctcg cattcagagc catcctgggt cagtatctgg aatctgatac agtacatgag
     1021 gaaatatttt ttacctatga gttattgacc attcagtata taggtgacta tttgatggac
     1081 aattaatttg ccgttttatt tttcattggc attttcttac aattttgctt ttagaaatac
     1141 cggctgcaaa agtgggtaaa acacaaaatt ttatctttga acaataatat tcaaaaatga
     1201 atttttctcc ggaacaagga agtttgttgg cttatgagca ttatggcatt actgctactg
     1261 ccagaccact caatggctat gacgaacaga actttttatt aactgcacag gacggcaata
     1321 aatatacttt caagattgca ggttcagaca tcagccgaac tttcctggag gcacaggccg
     1381 atgtgatgca tcatctggat cactcttcca ttcaaggcgg atttccggta tgtcttgaaa
     1441 acaagcaagg aaaagccatc actaccataa cttatgaagg taaagaatat catatcagaa
     1501 tacttaccta tttggaaggc catttttggg tagatgagaa aaacaaaaac aacttcctat
     1561 atgagagcct gggagaattc ctgggtgcta tggatcggtc gttggagggc atttgcccgg
     1621 atgcgacagt aagaaattat gtttgggaca ataaccgggc tccggaagct gaaaagaaac
     1681 tacacttcat ccgcgatcat gaaaaacgcc ggattgcagc gtatttcctg ttacagttca
     1741 ggacagaagt gacacctgta ttatcggaac tgaggcatgc atgtatacat ggtgatgcca
     1801 atgattacaa cctgctggtc cgcaatggaa gagtctccgg gcttatagat tttggtgata
     1861 tgatatattc tgccacgatc aacaatctcg ccatagcttg tgcctatgcc ctgttacata
     1921 ctgaggaccc gatcgaaaag gcaagcctga tcgtcaaagg ctatcacagc caataccctc
     1981 ttaccgagca agagatcgat atattatact acctgattgc cggacgactc tgtatcagcg
     2041 tcactcaatc tgccgaaaag tcttatagca acagcgaaaa tgagcatcac ttcctgtctg
     2101 agcaatcggc atggagattg ttgtaccaac taatccgcac caatccactc tatgcagccg
     2161 atcaattcag aaggtcatgc ggaatgatgc cctgtctgaa tccggaagac gattacagct
     2221 cactgttgtc tgaaaggaat cggtacatag gacgcaacct gagcatcagc tatcgtacac
     2281 cactgaaaat tgtcaagggc gccttgcaat atctttacga cgacaaaggc aaaacttatc
     2341 ttgactgtgt caacaatgtg agccatgtcg gccattgtca tccgggtgtc gtttatgcta
     2401 tccaaagtca ggccgctata ctcaatacca acacgcgcta cctgcacgac aatattacca
     2461 gctacgccaa agcccttgtt gacacacttc ctgagcgctt atcggtttgc tactttgtca
     2521 attcgggcag tgaagctaat gatctggcga tacgaatggc aagacatttt accggacaca
     2581 atgatattgt cgtcctcgac catgcctacc atggcacctc cacgttagcc atggaaatca
     2641 gcccgtacaa attcgatggt attggagggt ttggagccag gcattatatc cacaaagcag
     2701 cttcacctaa tctataccga ggcccctatg gcattgacga tgaggcagca ggagaaaaat
     2761 atgcgcaaag cgtcaaggaa attgtacaag gccttgaacg ggaagataaa tcccccgccg
     2821 catttatctg tgagacactt ctcggtgtgg gcggtcagat accattgcca acaggatatc
     2881 tgaaacaggt atatcaatac atccgctcag caggaggtgt atgcattgca gacgaagtgc
     2941 aagtcggttt cggcaggatc ggtgaagcat tctggggctt tgagcttcag gaagtcacac
     3001 ccgatatcgt agtcttgggc aagcccatcg gaaacggcca tccattggca gctgtcgtgg
     3061 taactcaggc tatcgcagat gccttcaaca atggtatgga atatttcaat acattcggtg
     3121 gcaatccggt ctctatggcg gctggtatgg ctgtgctcaa catcatccgg caagaagaac
     3181 ttcaacagca tgctctcaat accggaaatg gactaatgga tgacttgagg aaactgatgg
     3241 aaaaacatac catcatcggt gatgtacgtg gtcatggact gtttgtcggt gttgagctgg
     3301 taaggaatcg agtaagcaaa gagcccgctg tgccggaaat agacgtcatt gtggaaagaa
     3361 tgaagggacg tggcttcctg ctcagtactg acgggccttt gcacaacgta ctcaagatta
     3421 agccaccaat ggtcatcagc cgtcagaact gtgtcgattt ggtgcaggca ctggatgagg
     3481 ttttgtccga ggaattatga ttctgatgct gagctgaaag cttcatacca aggcttccaa
     3541 cctttcctgt ttttgatgac agactcgagg tattcatccc aggcggcttt gtcctgataa
     3601 ggatccttaa tgaccgtact gatgatggat gatgcaacat cggaaggttc taagttgcct
     3661 gtcccaaagt ggatggaatg ctgaatacct gtttgtagca cggataacac ttcagcagtg
     3721 gataatgaac tgcctggtga tttgatcttg cttttctttt cttctgtctc tccattcctt
     3781 aattccctga aaatggtaat caaatctgaa agatgctgca acggcacctt gtacttctcc
     3841 aaattcaggt cccgctggat ctgacccaat cggaaatgaa tgatatctat ttcttcctga
     3901 agggtctccg gcattgacag atacacgata ttaaaacgac gccgcaaagc ggcagaaaga
     3961 tcgtgcaccc ccttatcccg gtcgttggcg gttgcaatca cattgaatcc tttttgtgca
     4021 tatacttcca tatttaattc gggaatgctc atcatttttt cagaaagaac agtaatcagg
     4081 atgtcctgca cctcagttgg catccgtgtc aattcttcaa tccgggtgat gtagccgtct
     4141 tccatggctc tcaggacagg gcttttgacc aatgcacgct ccgagggtcc atgctgaagt
     4201 agttcggcat aattccaatg atatttcagt tgattctcgt ccatacccac cgtaccttgt
     4261 atcatcgaag tactaatccc cgaaatagca gctgccaggt gctcggaaag ccaggacttg
     4321 gcggtgccgg gcaaacccat gaggagcagg cccctgtcgg tcagtaatgt agctatgcac
     4381 aattccagta attttctatt gccgagatat ttaggctcga tgactacatc attatccagt
     4441 cggcctccga cgaggt
//
LOCUS       DAOQMC010000192         4453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9407_length_4453_cov_4.038427, whole genome shotgun sequence.
ACCESSION   DAOQMC010000192 DAOQMC010000000
VERSION     DAOQMC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9407_length_4453_cov_4.038427"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2549
                     /locus_tag="PKV31_07840"
     CDS             <1..2549
                     /locus_tag="PKV31_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB92808.1"
                     /translation="YDPYSSSATLVANERSFGKRVFPGYDFGNAQIVVNFGADFLGTW
                     VSPVEYTNAWTKNRKIKNFDKPEISRLIQYESNMSYTGSNADNRLLIKPSEQGLAIAK
                     LYNAVASLTGGSQLSVSGTLKNQKAEKSFAAVAKNLVDNKNAGKASLVLSDSNNIAEQ
                     IIVNKINDLLGNYGRTIDLNNYSNQRKGIDSDIVGLIKEMNAGSVSAVFILGDANPVF
                     DLPEGKDFAAGLKKTGLSVSLSTVPNETSAACGYVAPSNHFLESWGDAQPRKNNYYIM
                     QPTIAPLFATRQAEQSLLAWAKTPVDDFSTYMQGVWRSSLFGLQSEYSDFQRFWDNTL
                     HNGLIELASQSGAGSVAVDVSGLGSAVTKPGSGLEVKYYEPIGVGYGAYANNPWLQEM
                     PDPVTRTVWTSIIHIPLSWDGDSKFNYYQNLKKDGLFVDLSSGKTAVKDVPVVRQFGQ
                     MAETFAMPVGYGRTHGNASSMGVGNYIINTLTRDADGNLRYWNDNVKVEPNGGEDKHF
                     ASVQLHHTLGVRGKDKSNKEINVDEKAIMTIGSGFQGGLTDRSILKSADLSNFAEKVE
                     KLKEFRATAAKLNNETLYPGFDHLYKQGHHWGMSIDLNACTGCGACQVACISENNVPI
                     VGKKEVHRQHEMTWLRIDRYYYGDVESPNVFYQPMMCQHCDNAPCENVCPVNATNHSA
                     EGLNQMAYNRCVGTRYCANNCPFKVRRFNWADYTTADLFGRNELNVMPDGESLFYEDN
                     LTRMVLNPDVTVRSRGVIEKCSLCVQRIQEGKLRAKAEMRPLKETDIKPACATACPAG
                     AITFGDQNMKDGILNKEWDEPTTYFVLEEINVKGKIGYKMRVTNKNADLS"
     gene            2612..4042
                     /gene="nrfD"
                     /locus_tag="PKV31_07845"
     CDS             2612..4042
                     /gene="nrfD"
                     /locus_tag="PKV31_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNB92809.1"
                     /translation="MSIVVSPTREPLIFGGKTYHQITEDICTPSEKAPTRQWLIGFII
                     AVFFLAVFVFCVGWQIYYGVGAWNLNRTIGWGYDITNFVWWIGIGHAGTLISAILLLF
                     RQKWRTGVNRAAEAMTIFAVICAFMFPVIHVGRIWLLFYFIPLPNTRGPLWVNFNSPL
                     LWDVFAISTYFTVSLLFWYTGLVPDFATLRDRASGFRRKLYNFLSFGWTGAAKHWQRF
                     ESLSLVLAGLATPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVQTL
                     MLVTRKALKLEDYITINHIESMNKVILLTGTVVGIAYLTELFISWYSQYVYEQFAFAN
                     RAAGPFWWSYFGMMACNVLSPQVFWSKKIRRSLWWTFFMSIFVNIGMWFERFVIIVTT
                     LARDYLPSSWSYYYPSWNEIGIYMGTFGLFFTLYLIFVRIAPVVAIAEVKSILKSSGD
                     QYRGKNRGYNEKNEVVVAHSDHEEHH"
     gene            4067..>4453
                     /locus_tag="PKV31_07850"
     CDS             4067..>4453
                     /locus_tag="PKV31_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNB92810.1"
                     /translation="MASKNKEVLFGLFDDETQLLSAVADARKSHMEIDDVYTPFPVHG
                     LDPLLGLEESRLHIAGFFYGLTGTATAFFGISWIFTRDWPTIFGGKPYFSFPAFVPIM
                     FELTVLFASIGMVVTFYTICGMGPGVK"
BASE COUNT         1261 a          808 c         1044 g         1340 t
ORIGIN      
        1 cctatgatcc atattcttcc tccgcgaccc ttgttgcaaa tgaacgatca ttcggtaaac
       61 gtgttttccc gggctatgat tttggtaacg cccaaatagt ggtcaacttt ggagctgact
      121 ttctgggaac ctgggtgtct cctgtagaat acacaaatgc ctggaccaaa aatcgaaaaa
      181 tcaaaaactt cgacaaacct gaaatcagcc gtttgattca atatgagagt aatatgtctt
      241 acaccggctc caatgccgac aacagactgt tgatcaaacc atctgaacag ggattggcta
      301 ttgctaagct atacaatgca gttgcgtctt tgacaggagg tagtcaactg tcagtcagtg
      361 gcactttaaa aaatcaaaag gctgaaaaat cttttgctgc agtagcaaag aatttggtgg
      421 ataataaaaa tgccggaaag gcctctttgg tactctctga ttctaataat atcgcggagc
      481 aaatcattgt aaataagatc aatgatttgt tgggtaacta cggtcgtacc attgacctca
      541 acaattactc taatcaaaga aagggtatag atagtgatat tgttgggctg ataaaagaaa
      601 tgaatgccgg aagcgtcagc gcagtattca tccttggtga tgcaaatcct gtgtttgatc
      661 tgcctgaggg caaagatttt gctgcagggt taaagaaaac cggattgtcc gtttcactat
      721 caacagttcc aaatgagacc agtgcagctt gtggctatgt tgcaccttcc aaccacttcc
      781 tggagtcatg gggcgatgct cagccaagaa agaataatta ttatattatg cagcccacga
      841 ttgcaccctt gtttgctaca cggcaagcag agcaatcgct tctggcatgg gccaagaccc
      901 cggtagatga tttctctaca tatatgcagg gagtatggag atcctccctg tttggattac
      961 agagtgaata ttctgatttt cagagatttt gggataatac cttgcataat ggattgattg
     1021 aacttgcttc tcaaagtggg gcaggttccg ttgctgttga tgtttcagga ctaggtagcg
     1081 cagtcaccaa gccgggttca ggcctcgaag tgaaatatta tgagccgatt ggcgtaggct
     1141 atggtgctta tgcaaataat ccctggcttc aggaaatgcc ggaccctgtt acgaggacgg
     1201 tatggacaag tataatccat attccattgt catgggatgg ggatagcaaa tttaactact
     1261 atcaaaacct gaaaaaagat ggtcttttcg ttgatctctc atcgggaaag actgcggtaa
     1321 aggatgtacc tgtggtacgc caatttggtc agatggccga aacattcgcc atgcctgttg
     1381 ggtacggacg tacacatggt aatgcaagtt ccatgggtgt tggtaattat atcatcaaca
     1441 cgctgactcg agatgctgat ggtaacctca gatattggaa tgataatgtg aaggttgaac
     1501 caaatggtgg cgaggacaaa catttcgctt ctgttcagtt gcatcacact ttgggagtaa
     1561 gaggtaagga taagtctaat aaggaaatca atgtcgatga aaaagctatc atgactattg
     1621 gttccggatt tcagggagga ttaaccgacc ggagtatctt aaagtcagca gatcttagca
     1681 attttgcaga aaaggtcgaa aaattgaagg agttcagggc aacagctgcc aaactgaata
     1741 atgaaaccct ctatcctgga tttgaccact tgtataagca aggccaccat tgggggatga
     1801 gtatcgacct gaatgcatgc acaggttgcg gtgcttgtca ggttgcctgt atctcagaaa
     1861 acaatgtacc gatagtgggt aagaaggaag ttcatcgcca gcatgaaatg acctggttac
     1921 gaatcgaccg atattattat ggtgatgtcg aatctcccaa tgtattttac cagccaatga
     1981 tgtgccagca ttgtgataat gcaccatgtg aaaacgtttg tccggtcaat gctaccaacc
     2041 acagtgctga aggtctcaac cagatggcct ataacagatg tgtgggtacg cgttattgtg
     2101 ctaacaactg tccattcaag gttagaaggt ttaattgggc agattatacc acagcggatt
     2161 tatttggcag gaatgaatta aatgtaatgc ctgatggtga gtccctgttc tatgaggaca
     2221 accttaccag aatggtcttg aatccggatg tgacagtgcg gtcaagaggt gttatagaaa
     2281 aatgctctct ttgtgtacag aggattcagg aaggcaagtt gagggctaaa gcagagatga
     2341 ggcctttgaa ggaaactgat atcaagccgg catgtgctac cgcttgtcct gccggagcga
     2401 taacctttgg cgatcaaaat atgaaggatg gtattctcaa taaagaatgg gatgagccta
     2461 caacttactt tgttttggaa gaaattaacg taaagggaaa gattgggtat aaaatgcgcg
     2521 tgactaataa aaatgcggat ttaagttgat aattagaata atatatagag atatatttta
     2581 atattataaa taacagaata ataaaacgaa gatgtctata gttgtttcac caacaagaga
     2641 accattaatt ttcgggggca agacgtatca tcaaataacg gaagatatat gcactccctc
     2701 cgaaaaagca ccaaccaggc aatggttgat aggatttatt attgcggtat tttttttggc
     2761 agtatttgtt ttctgtgtgg gatggcaaat ttactacggt gttggggcct ggaacttaaa
     2821 cagaacgatc ggatggggtt acgacattac taactttgtt tggtggatcg gtataggtca
     2881 cgcagggact ttgatttcag caattctttt gctgttccgt caaaaatggc gtaccggtgt
     2941 aaatagggcg gctgaggcaa tgacaatctt tgcggtaatt tgtgcgttta tgttccctgt
     3001 tattcacgtg gggcgtattt ggttattgtt ttattttatt cccttaccaa atacaagagg
     3061 tcctttatgg gtcaatttta actcaccact tctttgggac gtttttgcaa tctctacata
     3121 ttttacagtt tcgcttttgt tttggtacac cggtctggtt cctgactttg caacgctcag
     3181 agacagagca agtggattta gaaggaaatt gtacaatttc ttgtcttttg gttggacagg
     3241 tgcagcaaag cattggcaga gatttgagtc tttatcctta gtattggcag ggcttgctac
     3301 accgttggta ctttccgtac atactatcgt atcctttgac tttgccactt cagttatccc
     3361 gggatggcac actaccattt tccctcccta ttttgttgcc ggtgcaattt tctcaggctt
     3421 tgcaatggtt caaacattaa tgttagtgac ccgaaaagct ttgaaactgg aagattacat
     3481 tacaatcaat catattgaat ctatgaataa ggtaatccta cttactggta ctgtagtggg
     3541 aattgcctac cttacagagt tatttatatc ttggtattcc cagtatgtat atgagcaatt
     3601 tgcctttgcc aatcgcgccg ccgggccttt ttggtggtca tattttggga tgatggcttg
     3661 taatgttctt tcccctcaag tattttggtc aaagaaaatc agaaggagtc tttggtggac
     3721 gtttttcatg tcaatttttg tgaatatcgg gatgtggttt gagcgatttg taattatagt
     3781 aacaacactg gcaagagatt atttaccatc cagttggagt tattattatc catcctggaa
     3841 cgaaatagga atatatatgg ggacttttgg tttgttcttt acattatatc tcatatttgt
     3901 ccgtattgct cctgttgtgg ctattgctga agtaaaatca atccttaaat cttctggaga
     3961 tcaatacaga ggaaagaatc gcggatataa tgaaaagaat gaggttgtgg tagctcattc
     4021 tgatcacgaa gaacatcatt aattagtagt taaagaatat agataaatgg catcaaaaaa
     4081 taaagaagta ttgttcgggt tgttcgacga tgaaacccaa ctgttgtcag ccgttgctga
     4141 tgcacggaag tctcatatgg aaattgacga tgtctatact ccttttccgg tgcatggatt
     4201 ggatccgttg ttaggcttgg aagagtcaag actgcatatt gccggatttt tctatggatt
     4261 gacagggact gcgacagctt tttttggaat ttcatggata tttacaagag attggccgac
     4321 catcttcgga ggtaagcctt acttctcatt ccctgcattt gtaccgataa tgtttgagct
     4381 gacagtgttg tttgcctcta taggaatggt agtgactttt tatacaatct gcggaatggg
     4441 ccctggagtc aag
//
LOCUS       DAOQMC010000193         4430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9484_length_4430_cov_4.241371, whole genome shotgun sequence.
ACCESSION   DAOQMC010000193 DAOQMC010000000
VERSION     DAOQMC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9484_length_4430_cov_4.241371"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2881)
                     /gene="dnaE"
                     /locus_tag="PKV31_07855"
     CDS             complement(<1..2881)
                     /gene="dnaE"
                     /locus_tag="PKV31_07855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNB92811.1"
                     /translation="MASFAHLHCHTQFSLLDGASDISLLYKKAVADKMPGIAITDHGN
                     MFGVFKFVSEAAKYNVKGQDPVIKPIVGCEFYLVEDRFKRQFTKTLKDKRYHQLMIAK
                     NEVGYKNLCKLSSLGYIEGLYGKYPRIDKKLVEQYHEGIIATSCCLGAIIPQLILEDK
                     IEEAEEELKWWVDLFDEDYYIELQRHKIPDQEKVNQVLLRFARKYNLKVIASNDAHYV
                     DREDSNAHDILLCVNTGEKQATPKSDGFNVDESDGRGTRFGFYNDEFYFKTTDEMTHL
                     FHDVPEAIENTLDIVEKVELLDLNKNILLPHYTIPEKFKTQDEYLRHITYLGAKERYK
                     ELTPEVIERLDFELGVIENMGFAGYFLIVADFIRAGRDIGVFVGPGRGSAAGSAVAYC
                     IGITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKVIDYVIEKYGKNQVAQII
                     TYGTMAAKSSIKDVARVLDLPLPQSNELTKMFPEKLGISLNRVLKAPLTGENSLEEHD
                     KLSTDDMAKVNELRQIFYNGTDLRSMVLLEALKLEGSVRNVGVHAAGVIIAPTNLMDI
                     LPVATAKDSELLVTQYDGKVVESAGVIKMDFLGLKTLSIIKDCLQYIKENHKVEIDID
                     AIPLDDGPTFELFQRGDTFGTFQFESAGMRKYLKELKPDRLEDLISMNALYRPGPMDY
                     IPSYINRKHGLEEVTYDIDEMKGILEETYGITVYQEQVMLLAQQLAGFSKGDADGLRK
                     AMGKKDRKTLDSLKGKFMEGIRARNFNEAKANKVWTDWEKFAAYAFNKSHSTCYAFIA
                     YQTAYLKANYLPEFMAANLKHQTNIEKITQYIEQCKASDINVLGPDVNESKIAFAVNA
                     RGDIRFGLSSIKGIGDGPSQDILDARGSEPFADIYDFVVRVNARSINKKTMEALVYSG
                     AFDCFNFEREAYFSANDKDLTFIEQLIRYAAAMKDMQEGSTMSLFE"
     gene            complement(3265..>4430)
                     /locus_tag="PKV31_07860"
     CDS             complement(3265..>4430)
                     /locus_tag="PKV31_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HNB92812.1"
                     /translation="VRIPVAPLWGMIDIVMEKIRLTREAGKKIDGHAPGLRGEDAMRY
                     FEAGISTDHECYELDEAIGKLRLGVHILIREGSAARNFEALIPAIADFPGQIMFCSDD
                     KHPDSLLLGHINQLVSRAVRKGYDLFDVLNAACINPVLHYGLEHGMVRPGDSADFILV
                     NDLEEFNVIKTYIRGQLVAENGKSFIPSRSKTTINHFNARPIDVTSLADQQSGEVSVI
                     ECLDGQLITNHLRMPSKECTADNDVLKVAVINRYREAQPALGRIRNFGLKSGALACTV
                     AHDSHNIIAVGVDDASMARVINELIKSKGGLAATDGTDMKILPLPVAGLMSDGDAWQV
                     SRDYTEIDDFAKQRCGSTLRAPFMSLSFMALPVIPHLKMTDKGLFDVDTFAFA"
BASE COUNT         1201 a         1072 c          840 g         1317 t
ORIGIN      
        1 cttcaaaaag actcattgtg cttccttcct gcatatcttt cattgccgca gcatacctga
       61 ttaattgctc gatgaaggtc aaatccttgt cgttggcaga aaaatatgct tctctttcaa
      121 agttgaagca atcaaatgcc ccgctataca ccagtgcttc catggtcttc ttattgatgg
      181 acctggcatt gacacgcacg acaaaatcat agatatcggc aaatggttca ctgcctcttg
      241 catccaggat atcctgagac gggccgtcgc caataccctt tatggaactg agaccgaaac
      301 ggatatctcc tctggcatta actgcaaatg caattttact ttcattgacg tcaggtccaa
      361 gcacattgat atctgaagcc ttgcattgtt caatatactg ggttatcttt tctatgttgg
      421 tctgatgctt aaggttggcc gccataaact ccggcaggta attggccttc agataggcag
      481 tctgatatgc tatgaaggca taacaggtcg aatgcgactt attgaatgcg tatgccgcaa
      541 atttctccca gtcagtccac actttattgg ctttggcctc attgaagttt ctcgccctta
      601 tgccctccat gaatttacct ttcaatgagt ccagggtttt cctatctttc tttcccatag
      661 ctttgcgcaa gccatctgca tcacctttgg agaaacccgc taattgctgg gcaagaagca
      721 ttacctgttc ctgatatact gtgatgccat aggtttcttc cagaatacct ttcatctcgt
      781 caatatcata agttacttct tccagtccgt gtttacggtt gatatacgaa ggtatgtaat
      841 ccatcgggcc cggtctgtac aaggcattca tagatatcaa atcctccaat cggtcgggtt
      901 ttagctcctt aagatatttc cgcatacctg cactttcaaa ctggaaggta ccgaaagtat
      961 ccccacgctg aaacaattca aaggttggtc catcatcaag cgggatggcg tcaatatcta
     1021 tctccacttt atgattttcc ttgatgtact gcaagcaatc tttgatgata cttaaggttt
     1081 taagcccaag gaagtccatt ttgataactc cggcagattc aactaccttt ccatcatact
     1141 gtgtcaccaa cagttctgaa tccttggcag ttgctaccgg aaggatgtcc atgaggttgg
     1201 ttggtgcgat gattacacct gctgcatgga cgcccacatt tctcaccgaa ccttccagct
     1261 tcagagcttc cagcaaaacc attgagcgca gatcggttcc attatagaaa atctggcgga
     1321 gttcatttac ctttgccata tcatccgtac ttagcttgtc atgctcttca agactattct
     1381 cacctgtcaa tggtgctttg agtaccctgt tcaaggatat gcccaatttt tcagggaaca
     1441 ttttggtcag ctcattcgat tgaggcaatg gtaaatccaa aactctggcg acatccttaa
     1501 tggagctctt ggctgccata gtaccatagg tgatgatctg cgcaacctga tttttaccgt
     1561 atttctcaat gacataatca atcactttct gccttccttc gtcatcaaag tcagtatcaa
     1621 tatccggcat tgacttccta tccggattca ggaatcgctc aaagagcaga ttgtacttaa
     1681 tcggatcaat attggtaata ccgatgcaat aagcgaccgc acttccggca gctgaacccc
     1741 gtcccggacc tacaaagaca ccaatatccc tcccagccct gataaaatct gctacgataa
     1801 ggaagtaacc cgcaaagccc atgttttcaa ttacgccaag ttcaaagtca agcctttcaa
     1861 taacctcggg tgtcaattcc ttgtatcttt cctttgctcc cagataggta atgtgtctca
     1921 aatattcgtc ctgtgtctta aatttttcag gaattgtata atgaggcagc agaatatttt
     1981 tatttaaatc cagtaattcg accttttcta caatatccag tgtattttca atcgcttccg
     2041 gcacatcgtg aaaaagatga gtcatttcat ccgttgtttt gaaatagaat tcatcattat
     2101 aaaagccaaa tctggtgccc ctgccatcac tttcatccac attgaaacca tctgatttgg
     2161 gtgttgcctg cttttcaccg gtattcacac ataacaggat atcgtgggca ttgctgtcct
     2221 cccgatccac atagtgagca tcgttggaag caatgacttt caggttatac ttcctggcaa
     2281 accgaaggag tacctgattt actttttcct ggtccggtat cttatgccgt tgtaattcga
     2341 tataataatc ttcgtcgaac agatcaaccc accactttaa ttcttcctca gcttcctcaa
     2401 tcttgtcctc gagaatcaat tgagggatga tggcacccag gcagcatgaa gtcgcgataa
     2461 ttccttcgtg atactgctca acgagctttt tatcaattct gggatatttg ccgtataatc
     2521 cttcaatgta accaagagag gacaatttac acaggttttt gtaccctact tcgttcttcg
     2581 caatcattag ctgatgatat cgcttgtctt tcaacgtctt ggtaaactgt cgtttgaagc
     2641 gatcttccac caaataaaat tcacagccta caataggctt gatcaccgga tcctgacctt
     2701 taacattgta tttagcggcc tctgatacaa acttaaagac gccgaacata ttaccgtggt
     2761 ccgtgatggc gatcccgggc attttatcag ccacagcctt tttatataac aggctaatat
     2821 ccgatgctcc atccagcagg gaaaattgag tatgacagtg aaggtgtgca aatgaggcca
     2881 tagtcttgtt tttaaatcaa attgtatatg ttagctcaaa agatgtttta tgattcacat
     2941 tcaaaaaata aaaatatgat taatcgcaat atgaaatgac aaatctaatg aatctaacca
     3001 atcatttttt tattatgaag catcaaatta tacccgggca aacgggtttg tcccaaatag
     3061 aaccgactgg aatacacgtg caaattctct catctgctat ccataatatc caaaagattc
     3121 actatgattt cgggaatttc ggcttgtcta ccacccttta tttcccatgc aattgtttta
     3181 ataaacagga aggaagatag ataagtttca cacattttaa ttttttttaa taaatgtttt
     3241 tccttttcca aaacaccttg gatttcaggc aaaggcaaac gtatctacgt caaaaaggcc
     3301 tttgtccgtc atcttcaagt gaggtataac cggaagggcc atgaatgaca aagacatgaa
     3361 aggagccctg agggtagaac cacaacgttg ttttgcaaaa tcatcgatct cggtataatc
     3421 acgacttact tgccatgcat cgccatcact cataaggccg gctaccggca gaggaagaat
     3481 tttcatatcc gtgccgtcag ttgcagccag accgcccttg gatttaatca gctcattgat
     3541 tacccttgcc atcgatgcat catcgacacc tacggcaatg atattgtgac tgtcatgtgc
     3601 taccgtacag gcaagggcac cactcttcaa gccgaagttc ctgatacggc ccagtgcagg
     3661 ttgggcttcc cggtaccgat taatcaccgc aaccttcaac acatcattat cggcagtgca
     3721 ttctttcgat ggcatccgca ggtgattggt aataagctga ccgtcaagac actcgataac
     3781 actgacctct ccagactgct gatcggcaag agaagttacg tctatgggtc gggcattgaa
     3841 gtggttgatc gtagttttac ttcttgacgg aatgaaagat ttaccatttt cagcaactaa
     3901 ttgtcccctg atgtaggttt tgatcacatt gaattcttcc agatcattga ccaggataaa
     3961 atcagcggaa tcaccgggtc tcaccatgcc gtgctcaagt ccgtaatgca ataccggatt
     4021 gatacaagct gcattaagca catcaaacaa atcatagcct ttcctgaccg cccgactgac
     4081 caattggttg atatggccga gcaacaggct atccggatgt ttgtcgtcgg agcaaaacat
     4141 aatttgtccc ggaaaatcag caatagcggg gatcaacgct tcaaaattcc ttgcagcact
     4201 gccttcgcgt atcaggatat ggacgccaag tcttaattta cctatcgctt catctaattc
     4261 ataacattca tgatccgttg aaatgcctgc ctcaaaatac ctcatggcgt cttcaccccg
     4321 caatccgggt gcatggccat caattttttt tccggcttcg cgggtcaatc tgattttctc
     4381 catcacaatg tcaatcattc cccacaaagg agcaaccggt attctcacag
//
LOCUS       DAOQMC010000194         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9663_length_4379_cov_5.355458, whole genome shotgun sequence.
ACCESSION   DAOQMC010000194 DAOQMC010000000
VERSION     DAOQMC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9663_length_4379_cov_5.355458"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKV31_07865"
     CDS             <1..525
                     /locus_tag="PKV31_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB92813.1"
                     /translation="VPAIPDPVFAGTWIEVGKPSFFLLVFAVFMLAAAFAMTRKKKVE
                     EDNTTNANSLMITIAGLFEGLLTGLVGAGGGFLIIPALVLLGKVEMKKAIGSSLLIIA
                     VKSLMGFTGDLHNNVNIEWKLLIPFTLIAIGGIITGSRLSRRISNEKLKPLFGYFILI
                     MGLIILAKELLIGR"
     gene            625..2007
                     /locus_tag="PKV31_07870"
     CDS             625..2007
                     /locus_tag="PKV31_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB92814.1"
                     /translation="MKVEQIYTGCLAQGAYYIKSKDEAAIIDPLREIKPYLERAEKDG
                     VKIKYIFETHFHADFVSGHIDLSKATGAPIVYGPCADPAFDALIACDGQEFKVGDVTI
                     KALHTPGHTMESTCYLLCDSDGKQQCLFSGDTLFLGDVGRPDLAQVEGKLTVEDLAGH
                     LYDSLMNKIAILPDTLIIYPAHGAGSACGKNMMKETFDTLGNQKQVNYALKQPDKQTF
                     IREVLSGLTPPPKYFGLNVMMNKSGYQSIDTVLSRGMTGLDPEEFERIANDCDALVLD
                     VRSTQEFSNGFIPRSINISLDGKFAPWVGAMIVDVNTPILLVTDAGKEEETSIRLSRV
                     GFDNQLGHLRGGIAAWEKAGKETDKVERISPNQFLREISEDASVVFDIRREDEYTAGH
                     VSKAVNMPLEEINHWIEKASNAQNLYIHCAGGYRSMIAASILKSRGIHNFKEVDGGFN
                     AIKRAKEGIA"
     gene            2241..2663
                     /gene="ssb"
                     /locus_tag="PKV31_07875"
     CDS             2241..2663
                     /gene="ssb"
                     /locus_tag="PKV31_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB92815.1"
                     /translation="MVNKVILIGNLGMNPEVHQFETNSVARFSLATSDTYIDKNGERQ
                     STTEWHRVVVWGKQAENAQRYLKKGSKVYVEGKITYNKYTDKNNIERTATEIKADLIR
                     YLDSAGGATAGGGMTDQTASEPSQAGPDPDTTIDDLPF"
     gene            2926..3489
                     /locus_tag="PKV31_07880"
     CDS             2926..3489
                     /locus_tag="PKV31_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNB92816.1"
                     /translation="MKIRQFIALGLLFWSILSFGQNEMLEVQVSKDSVYFGNQVKVTF
                     IASNISGKFIPPAFDGFDIINGPFTSSSYSFVNGESSRKTTYTYLLQPQRIGEIIIGK
                     AGFNSEGKELFTNEIPIYVRDNPNGIREDAEKQDMKQKSKSPFDNFGKFDDFFFNMPD
                     FDGFDIVPKEKTPPPPKKTYKFKTETL"
     gene            3504..>4379
                     /locus_tag="PKV31_07885"
     CDS             3504..>4379
                     /locus_tag="PKV31_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNB92817.1"
                     /translation="MSRLTNSLVLLLLPFLSIGQDVRFYAETNSRSVPVDGYFEVHFK
                     VDNAASDKIVPPDFQGFTVLGGPSYSSQTSIINGSVTRSSSVTYVLQPKSIGIVQIGQ
                     ATATINGKKYTSQAISIEVTKAESASGLSSMSKKGEEVFVQIEVDQKEPYIGQQVILD
                     YVIYSRISVNSFNFNSESKYTGIYVKPFKNFDAGVKTRTINGYQYRRQIMRRLALFPQ
                     QSGNILIEPAVITLGVPMEDDPFGNFFQSSRPKQVRTNSVELQVKSLPQPIPADFAGV
                     VGEFQYSVTCDKSELT"
BASE COUNT         1370 a          876 c          984 g         1149 t
ORIGIN      
        1 gtacccgcca ttcccgatcc ggtgtttgcc ggaacctgga tagaggtagg caagccgagt
       61 ttcttcctgt tggtctttgc cgtattcatg ttggcagcgg catttgccat gacaagaaaa
      121 aagaaagtcg aagaggacaa taccacaaat gctaattcat tgatgattac cattgccggt
      181 ttgtttgaag gcctgctgac aggacttgta ggtgctggtg gtggattttt gatcattcct
      241 gccctggtgt tactgggtaa ggtcgaaatg aagaaggcta taggatcaag tttgctgatt
      301 atagccgtaa aatctctgat gggatttaca ggcgatctgc ataacaatgt gaatattgaa
      361 tggaaactgt tgataccttt tacgctaata gcgataggcg gaattattac aggcagccga
      421 ttatccagac gcatttcgaa cgaaaagctc aaacccctgt ttggttattt tatactgatc
      481 atggggttaa tcattctggc aaaagagtta ttgataggaa ggtaaggtgg acttattgaa
      541 gggaatttga ataggaacat tgacttcaaa taagggataa acaaggacct ttagaatatt
      601 taaagaaaga ttaatatatt gtagatgaaa gtagaacaaa tatacacggg atgcctcgct
      661 cagggcgcgt attacatcaa aagcaaggat gaggcagcaa tcatagatcc tctcagagaa
      721 attaaaccgt acctggaaag agcagaaaaa gatggtgtga agatcaagta catctttgag
      781 acacactttc acgctgattt tgtatccggg catatcgacc taagcaaagc aacgggagca
      841 cccattgtat atggcccttg cgccgatccc gcatttgatg cattgatcgc ctgtgacgga
      901 caagaattta aggtaggtga tgttacaatc aaggcactcc ataccccggg ccacacgatg
      961 gaaagtacct gttatttgtt gtgcgattct gacggaaaac aacagtgtct gtttagtggc
     1021 gacaccttgt tcctgggtga tgtagggcgt ccggatcttg ctcaggtaga gggcaagctt
     1081 accgttgagg atctggcagg ccacctgtat gacagcctga tgaacaagat agccatcctg
     1141 ccggacacgc taattattta tcctgcacac ggagcaggta gcgcttgtgg aaagaatatg
     1201 atgaaggaaa ctttcgacac attgggtaat caaaagcagg tcaattatgc gctcaaacag
     1261 cctgacaagc aaactttcat ccgagaggta ttgtcaggtt tgacgccacc tccaaaatac
     1321 ttcggcttga atgtaatgat gaacaaatca ggctaccaaa gtattgatac agtcttaagt
     1381 cggggtatga cagggctgga tcctgaagaa tttgagcgaa ttgccaatga ctgtgatgca
     1441 ttggtactgg acgtccgtag cacacaggaa ttttcgaacg gttttatacc caggtcgatt
     1501 aacatctccc ttgatgggaa gttcgcacct tgggtaggag ctatgattgt cgatgtcaat
     1561 acaccgatcc ttttggtgac cgatgcagga aaggaagaag aaacatccat aaggctaagc
     1621 agagttggat ttgacaatca actgggacac ctgagaggag gaattgcagc ctgggaaaaa
     1681 gccggcaagg aaacagacaa ggtagaaaga atttctccaa accaattcct ccgggaaatt
     1741 tctgaagatg catctgttgt ctttgatatc cgcagagaag atgaatatac agcaggacat
     1801 gtatcaaaag ccgtcaatat gcctttggaa gaaatcaacc attggattga aaaggcatca
     1861 aatgcccaaa acctatacat acactgtgcc ggaggatata gaagtatgat tgccgcatcg
     1921 atccttaaat caagaggtat ccataatttc aaggaagtgg atggtggctt caatgctatc
     1981 aagcgtgcaa aggagggtat tgcttaagta taaagctgag taaatcccgg catggcatgg
     2041 caatatttga ccttctattg aataaaaaaa ccgactcctt cattattagg attttcggta
     2101 tttaaaagaa tgtcaaagca ttttgttctg ttcggtaatt tattcatcca gataaaatca
     2161 ggaataaaat aaccgcattc ttttaaaatg tcgtatcttg taaccttata tttaaaactt
     2221 aaaattcaaa tctctaaact atggttaaca aagtaattct tatcggaaac ttaggcatga
     2281 atcctgaagt gcatcagttc gagactaatt cagtcgctcg tttttctctt gccacaagtg
     2341 atacctacat cgacaaaaat ggtgaacgac agtctacaac agaatggcac agggtcgttg
     2401 tatggggcaa acaggctgaa aacgcccaac gatacctgaa aaagggcagc aaggtatacg
     2461 tggaaggcaa aatcacttac aacaaatata ccgataaaaa taacatagag cgtacggcaa
     2521 cggaaatcaa ggcagactta ataaggtatc tggattcggc aggaggtgca acagcaggag
     2581 gaggaatgac agaccagact gcatctgaac ctagccaggc aggaccggat ccggatacta
     2641 caatcgatga tttgccgttc taagaaaagg aaaaggagag gtcgccaggc ttctcttttt
     2701 ttctgtaaat tatttgttat cgatatgttt cagccttgct agctgataag aatatacgtt
     2761 aatacgtagt cagagcacag gacacagata ctgaaatcaa atgttctgtt ttaactgatc
     2821 aagtagctat tgtcagagaa ttttccaaac aattggcgga cgaattgaat tgtatataaa
     2881 aaagaaaaat acatagttta tcaaataaat attggattac agaaaatgaa aatcagacaa
     2941 tttatcgcat tgggtttact gttctggtcg attctttctt ttggccagaa tgaaatgctt
     3001 gaagtgcagg tcagcaagga cagtgtgtac tttggcaatc aggttaaggt aaccttcata
     3061 gcaagtaata tttctggcaa attcattccc cctgccttcg atggcttcga tattattaac
     3121 ggacctttca cgtcgagcag ctattctttt gtcaatgggg aatccagtag aaagactact
     3181 tatacctacc tgttgcagcc ccaacggatc ggcgaaatca tcatcggcaa ggcaggattc
     3241 aactcagagg gaaaagagct ctttaccaat gaaatcccta tctatgtcag ggacaacccc
     3301 aatggtatcc gcgaggatgc tgaaaaacag gatatgaagc agaaatcaaa atcccccttt
     3361 gacaacttcg gcaaattcga tgatttcttc tttaatatgc ctgatttcga tggttttgat
     3421 attgtgccga aagagaaaac tcccccaccc cctaaaaaaa cctataaatt taaaacagaa
     3481 acattataac cccgatccgc tcaatgagcc gtctgacaaa ttctcttgtt ttattacttc
     3541 tcccatttct gtctatcggt caggatgtgc ggttttatgc ggaaaccaat tccagaagtg
     3601 tccccgtaga tggatatttt gaggttcact tcaaggtgga taatgctgca tccgataaga
     3661 tcgtcccccc tgactttcag ggatttacag tattgggcgg accgagctat tcctcacaga
     3721 cgagtataat taatggcagt gtaacgcgtt cttcttcggt tacctacgtc ctgcaaccaa
     3781 agagcattgg tatcgtccag attggtcagg ccacggctac catcaatggt aagaaatata
     3841 catcacaggc catttcgata gaagtgacca aggcagaatc tgcttcgggg cttagctcca
     3901 tgtccaaaaa aggagaagaa gtgtttgtcc aaattgaggt ggatcaaaag gagccctata
     3961 taggccaaca ggtaatcctg gattatgtca tatattcaag aattagtgtc aattcattca
     4021 atttcaacag tgaatcaaag tataccggaa tttatgtcaa accctttaag aatttcgatg
     4081 ccggagtaaa gacacgcacc atcaatggtt atcaatatag aagacaaatc atgcgaaggc
     4141 ttgcactttt tccacagcaa agcgggaata tacttattga gccggccgtc attacccttg
     4201 gcgtgccaat ggaagatgac cccttcggga acttctttca aagcagcaga ccaaaacaag
     4261 tacgcaccaa cagtgtagaa ctgcaagtca agtcgcttcc tcaaccgatt cctgctgact
     4321 ttgccggagt agtgggcgaa ttccaatact ccgtcacttg tgataaaagc gaattgacc
//
LOCUS       DAOQMC010000195         4347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9775_length_4347_cov_3.570363, whole genome shotgun sequence.
ACCESSION   DAOQMC010000195 DAOQMC010000000
VERSION     DAOQMC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9775_length_4347_cov_3.570363"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKV31_07890"
     CDS             <1..212
                     /locus_tag="PKV31_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92818.1"
                     /translation="VGREAASRLGSKGTQNGVNMLSELAKIYNKNNELRISQQYRDHI
                     FTHPDGRGYGTEVVIRIPKKYNYVV"
     gene            717..4331
                     /locus_tag="PKV31_07895"
     CDS             717..4331
                     /locus_tag="PKV31_07895"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNB92819.1"
                     /translation="MKSFLFLIFGCIIICNNLFSQSNQFCGFDARHELLLQSDSSYKE
                     NFQLWEEKLYNELKQGSPLQIRQTYTIPVVVHIITPPGTPIGTGNNLTDTEVEAGLAL
                     LNDAFANRGAFHTPEGQDIGIQFCLAKRTPDGQPTNGITRHESNLVADAACSSYGTSM
                     DNDAAIKKIVTWDCRQYLNIWLVTDLYNSSFGCSLAGFAYFPGAPCTLDGIIQESRYW
                     TTVGGTVVTAHETGHYFGLYHTFQGGCKNDDCLIDGDKVCDTPPDGSPSFAPCNTNSC
                     HTDTPDLPDDNSNYMDYSSCSPVHFTAGQKTRILSGLELGRPSLLTSIGCSPVLSLDV
                     GVRMSIDQKCKTKFCPIIELVNFGSTTITSGTFEIKLTPGSTFTINWTGNLSANNSIS
                     FPADCIDLAPGTYSATVHALTVNGSADENSANDYFTMSTVQVQNVIDVDFDYYNAFQL
                     RYYFRNNSKSFESCKWEFGDGTTSTETSPFHDFVNQGQYTITLTCYNSCGDSTKITKT
                     IEIQACSGQLRGRVQSLNDYIEPDEDICEIVQNGILYLRPELADIGYYWTFFSICDTI
                     CTGEGYTIKIRLKNDNASGGINAYDTGLEVIGEGENAGIFIMGLPWAIDFTRVWVGNN
                     YLTGLPQLIMKLGDWKTIEMELKNNVLSYKYEGSNFFQMEYPGKVCNIHNINLTFKGS
                     GSVDWIQVYDVNGNLVYEENFDDCNNLTKSIPCPKRPINAEVLVTDECRQPKVEIKNL
                     NGSDDIITTQVFPGVPVDANGRYELVNPGSYKFLLTRTCPLADSSFVLEVVPPMKDSL
                     IRFEQPLCNGSYGFINVSGVGGRPPYEFNINGKQSPTGVFHDLAPGNYEVIITDQNGC
                     SVKNNYTIQDPATPLSFAVDSFRYDLTCYNPTSYIQLGVSPATLNVYFTLDDSLQTNH
                     GYFENMGAGMHKISIHDESRCYNDTLYFEVRDLRKTTHTDLTYDICPGDSVEVAGNFY
                     SQVGTYNDTLVAYTGCDSILAISIHEYVKANAGHSLGIPGYCNNVEQVIKLDELIAGE
                     DKDGFWKVIQGDNNTFDSDNSTFNSFKASPGSYIFQYKVNGAKDCVSDSVMIELDVFD
                     GPKSILFSNDSLFNCRDIVALSAIPDIQSSDIASVQWYLGDKQIEGANGLQTIISDPR
                     SGIYRVRLVDKNGCELEARQHIRIEYSLPYFAPNVFSPNFDGINDVFNPSCALFNKLC
                     "
BASE COUNT         1303 a          903 c          937 g         1204 t
ORIGIN      
        1 gtgtagggcg tgaggcggca tcccgcctgg gatcaaaagg aacccaaaat ggggtaaaca
       61 tgctttcaga gcttgccaag atttacaata agaacaatga actccggatt tcacaacaat
      121 acagggatca tatcttcacc catcccgatg gcagagggta cggcacggaa gtcgtgatca
      181 ggattccaaa gaagtacaac tatgtggtgt agtgagacgt ggaaaatgta tgccttgtaa
      241 ggctcgttgt acaaccctta aaaccaggta aagcaagaga aaattttcgc cctcaaacct
      301 tttcataccc ataaataccc gtttcattag cgaaaatacc gtgttcactg ttcgacccgg
      361 attttgtatg tatatatata ataaattgcg ggtgaaataa ttttacttca ccatgtatga
      421 ccttcaacct tatcgaaata gcaatactaa agaagcatct gtcgaatccg gcctttacac
      481 tacagccttg ctttgtcgga attgggtgat ttctttattg tcaagtaaag gctatatttc
      541 atcgaagtat ttacaagaaa ttatcgcatc atccataaag aatataaaaa ttattaagat
      601 gaagtgtaga tcaggtttac atacattaaa agctgtacaa aagcataaca atgaaaccga
      661 ttttgtaaaa atagcttgtg atttagcaat aagaagcata ttttaaaacc attcgtatga
      721 aatctttttt attcctgata tttgggtgta taatcatttg caataatctt ttttctcaat
      781 ctaaccaatt ctgcggattt gatgcccgcc acgaattatt attgcaatcg gattcatcct
      841 acaaggaaaa ctttcaactt tgggaggaaa aactctataa cgaattaaaa caaggatcgc
      901 ctctacaaat aaggcaaacc tacaccatcc cagtcgtggt acatatcatc acacctcccg
      961 gaactccgat tggtaccgga aataacctca ccgatacgga agtggaagcc ggtctggccc
     1021 tgctcaatga cgcatttgcc aatagaggtg cctttcatac acctgaagga caagatatag
     1081 gaatacagtt ttgtctggcg aaacgcacac ctgatggtca gccgaccaat ggcattaccc
     1141 gacatgagag caatctggtg gcagatgccg cctgctcttc atatggaaca agtatggaca
     1201 atgacgctgc catcaaaaag attgtcacat gggactgcag gcaatacctg aatatttggc
     1261 ttgtaacgga cttgtataat tcctcctttg gatgttccct ggcaggcttt gcctactttc
     1321 ccggagcacc atgtaccctg gatggcatca ttcaggaaag ccggtactgg acaacggttg
     1381 gtggcacggt tgtcacagcc catgaaacgg gtcattattt tggcctttac cacacttttc
     1441 agggagggtg taaaaatgat gattgcctca ttgacggcga caaagtctgt gatactccac
     1501 ccgatggcag tccctcgttt gcaccctgta ataccaactc ctgccataca gatacacctg
     1561 accttcccga tgacaattcc aactatatgg attattcgtc ctgttcaccg gtacatttta
     1621 cagcgggtca gaagaccaga atcctatcag ggttggaact gggcaggccc agcctgttga
     1681 cttccattgg ttgcagcccg gtacttagct tagacgttgg tgtcagaatg agcattgacc
     1741 aaaaatgcaa aaccaaattt tgtcctatca tcgagttggt aaattttgga tcgaccacga
     1801 tcacttccgg aacctttgag atcaaattga caccgggcag cacattcacc atcaattgga
     1861 cgggcaatct ctcagcaaat aatagcatct cattccctgc agattgtata gatcttgctc
     1921 cgggaactta cagtgccact gtacatgctc ttaccgttaa tggatcagcc gatgaaaaca
     1981 gcgccaatga ctattttacc atgtcaacgg tccaggttca gaatgtcatt gatgttgact
     2041 ttgactacta taatgccttc cagctcagat attatttccg aaataattct aagtcttttg
     2101 agtcgtgcaa atgggagttc ggtgacggaa ctacaagtac ggagacgagt cccttccatg
     2161 attttgtaaa tcagggtcaa tacacaatca cccttacttg ctacaactct tgtggcgatt
     2221 caacaaaaat taccaagacc attgaaattc aagcttgctc aggtcagtta cgaggaagag
     2281 tacaaagcct taacgattac attgaaccgg atgaagatat ttgtgaaata gtacagaatg
     2341 gtatcttgta tcttagacct gaacttgccg acataggtta ttattggacc tttttttcta
     2401 tctgtgatac catctgtaca ggggaaggct ataccataaa gatccgactt aaaaacgata
     2461 atgcttccgg tggaatcaat gcatatgata ctgggctgga agtcattggc gaaggtgaaa
     2521 atgcagggat ttttatcatg ggtctaccct gggcgattga ttttaccaga gtctgggtag
     2581 gcaataatta cctgacaggt ttacctcaat tgattatgaa actaggtgat tggaaaacga
     2641 ttgagatgga gttgaaaaat aacgtgctga gttataaata cgaaggaagc aatttcttcc
     2701 agatggaata tccggggaag gtgtgtaata tacataacat caatttgacc ttcaaagggt
     2761 ccggttccgt ggattggata caggtatatg atgtaaacgg aaatctggtg tatgaagaga
     2821 attttgatga ttgcaataat ctgacaaagt ctattccatg tcccaagagg cccatcaatg
     2881 ccgaagtcct tgtaacggat gaatgccggc agccaaaagt tgaaatcaaa aacctgaatg
     2941 ggtcagatga cataataaca acccaagtgt tccctggtgt acctgtcgac gccaatggcc
     3001 ggtatgaact ggttaatccg ggctcctata aatttttact tacgagaaca tgtccgctgg
     3061 cagattcctc atttgtcctg gaggtagtgc cacccatgaa ggattcgctg attcgttttg
     3121 agcaaccctt gtgtaacgga tcttatggct tcatcaatgt ttcaggagtc ggtggaaggc
     3181 caccctatga attcaacatc aatggtaagc aaagtccaac cggtgtcttt catgatctgg
     3241 ctcccggaaa ttatgaagtt ataataacgg atcaaaacgg atgttctgtc aaaaacaatt
     3301 ataccataca ggatccggca acacctctct cctttgctgt ggatagcttc aggtatgatt
     3361 tgacttgtta taatccgact tcatacatac agcttggcgt ttctcctgca acactgaatg
     3421 tttattttac cctggatgat agcctgcaga ccaaccacgg gtattttgaa aacatgggtg
     3481 caggtatgca taaaatctca atccatgacg agtctcgatg ttataacgat actctttact
     3541 ttgaggtcag agatctcaga aagaccacac acacagatct gacctacgat atatgcccgg
     3601 gtgactctgt tgaggttgcc ggtaacttct atagtcaggt tggcacctat aatgacaccc
     3661 tggtagcata taccggatgt gacagtattc tggcgatcag tattcatgaa tatgtaaagg
     3721 cgaatgccgg acattctttg ggaataccgg gatattgcaa taatgttgaa caagtaatca
     3781 aactggatga attaattgca ggagaggata aggatggatt ttggaaggtg atacagggag
     3841 acaacaatac ttttgactca gacaacagca cttttaattc atttaaagca tctccgggga
     3901 gttatatttt ccagtataag gtcaatgggg cgaaggactg tgtaagtgac agtgtcatga
     3961 ttgagctgga tgtatttgat gggccaaaga gcatcctatt ttcaaatgat tcccttttca
     4021 attgtcggga tatagtcgca ctgtcagcta ttccggatat ccagtcttcg gatattgcgt
     4081 ctgtccaatg gtacttagga gataagcaaa tagaaggagc gaatgggctt caaacaataa
     4141 taagtgatcc ccgatcaggt atttatcggg tcaggctggt tgataaaaac ggatgtgaac
     4201 tggaggccag gcaacatatt agaatagaat attcccttcc atacttcgca ccgaatgtct
     4261 tctcaccgaa ttttgacggc atcaatgacg tgtttaaccc aagttgcgca ttattcaaca
     4321 aattatgtta aaaaacggtc aaagaac
//
LOCUS       DAOQMC010000196         4338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9809_length_4338_cov_5.617558, whole genome shotgun sequence.
ACCESSION   DAOQMC010000196 DAOQMC010000000
VERSION     DAOQMC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9809_length_4338_cov_5.617558"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..2037)
                     /locus_tag="PKV31_07900"
     CDS             complement(91..2037)
                     /locus_tag="PKV31_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNB92820.1"
                     /translation="MQQFLPELLSGSKYSSRLPIGKDEILKTFKYEELRRFYRDWYRP
                     DLMAVAVVGDITKEKAEQMIKNHFSAIKGPKNPRTREYAEINPFKEAKAMVVTDKEAT
                     SYQFILAFSGSRVKPEVTLNDYRTRIVRSLFTQMLNKRYSDLAHSANPPYIFASSDVG
                     GIFTERYEQLLLMAMPSSDIKGTIDASVAEFLRAQKYGFNASEFELAKKSTMSSIENR
                     YNERNTTESSVLIQNFISNYLNGGPLPGIENEYRLYKEFLPGITLEEVKAEASKWLSP
                     NVTQDYFALLMGPQVESGNVPDSRGLLEMVKSAFRQTVEDNIEKKASENLIENLPAPG
                     KVLSQQKNDLLGIVTYTLSNGVKVTVKKTDFKSDEILLEGVRKGGASNFGKEDKSNVQ
                     FMSDVMEAMGYGNFTPTELGDALTGKNITLTQSMDEYSDKVSGSCSVKDFSAFLELVH
                     LQMTSPRLDKDLYEGYLSKMKMQLMFLKANPQVSFIDTLFKVMYNNNPLRPIAVPTLD
                     DVEKIDPARIVELYKQEFGNAYSFHFFLTGNVDESNIVPLLEKFIACLPSTPYTPSFK
                     DNGVDIIAGTHTFEYKKGENQKSLIIDVTHGPIPYTEDLELQTGIVGDILTIRLLEEV
                     REKMGAIYGGTYVANIDRFPVSRY"
     gene            complement(2050..2430)
                     /locus_tag="PKV31_07905"
                     /pseudo
     CDS             complement(2050..2430)
                     /locus_tag="PKV31_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
     gene            2804..3532
                     /locus_tag="PKV31_07910"
     CDS             2804..3532
                     /locus_tag="PKV31_07910"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNB92821.1"
                     /translation="MKHFGLIGKSLKHSFSKKYFDEKFKAFGLLDFDFRLIEIPDIED
                     LPEKVKEYELSGFSVTIPYKEAIIPFCHSLSPEAEKICAVNCVKVTDNGWVGYNTDYV
                     GFRDTLPLDDLAKGGTAIVFGTGGASRAIIHVLSRLGLEIINVSTSSKKGTIHYQALK
                     DQIIKKALILVNCTPLGTFPEVDKNVPINFSAIHKGQICYDLVYNPDQTKFLRLSAQQ
                     GARTINGMKMLIIQAEEAWKIWNN"
     gene            complement(3577..>4338)
                     /locus_tag="PKV31_07915"
     CDS             complement(3577..>4338)
                     /locus_tag="PKV31_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB92822.1"
                     /translation="FINTSIAPTDPIVSYQWEFGANSNIGSSTQQNPPGVIYDGPGYK
                     SVSLTITSQDGCVHTFTKNFIAKCCIDPLKVSMAVQPYNEVKLGDPIVLTATTENAVG
                     STIFEWFPSDLITNCFNCREVTLVPVGNYIFTVTAQDQKGCISTDSLEIRVVDSKDVV
                     APNVFSPNYDGINDYFTVFPNRGAKFIKRLLVFNRWGACVYEGKELTPGDDSIGWDGT
                     FKGKECTPDVYAYFAEVEFLNGRIQIIKGDVTLVR"
BASE COUNT         1316 a          923 c          792 g         1307 t
ORIGIN      
        1 accgtcttcc ttaatttcac gtaccaattc attcaattta gtcaaaatag gtgttacatt
       61 ttccggcccg cattgcaact gagtagcgac ctaataacga gaaaccggaa agcgatcgat
      121 attggcaaca taggttccac catagatggc acccatcttt tctcgaactt cctccaatag
      181 cctaatagtc aatatatcac caacaattcc tgtttgcagt tcaaggtctt ccgtgtaagg
      241 aatcggccca tgagtcacgt caatgatcag acttttttgg ttttctcctt ttttatattc
      301 aaaagtatgg gttccggcaa taatatccac tccattatcc ttgaatgaag gcgtataagg
      361 tgttgatggc agacatgcaa taaatttctc caaaagtgga acaatattgg attcgtcgac
      421 attccccgtg agaaagaaat ggaaactata tgcattgcca aactcttgtt tatacagttc
      481 aacaattcgt gccggatcaa tcttttccac atcatccaaa gttggcactg ctataggtcg
      541 caatggatta ttattgtaca taaccttaaa cagggtatca ataaaggaaa cttgcggatt
      601 agctttaaga aacatgagct gcatcttcat tttactcaag tacccctcat acaaatcctt
      661 gtccaaacga ggtgaggtca tttgtaaatg gacaagttca agaaaagcag aaaaatcttt
      721 gacagaacaa ctgcctgaaa ccttgtcgga gtattcatcc atactttgag ttaatgtgat
      781 attcttccct gttagagcat ctcctagttc agtgggcgta aaatttccat atcccatcgc
      841 ctccatcaca tcactcataa attgtacatt gcttttatcc tcttttccaa agttggatgc
      901 tcctcctttt cgaactcctt caagcaagat ttcatcagat ttgaaatcag ttttcttaac
      961 agttacctta acaccattac tcaacgtata agtaacaatc ccaagtaaat cattcttttg
     1021 ctgacttaaa accttacccg gagcgggcag attttcaata agattttctg aggccttttt
     1081 ttcaatattg tcttcgacgg tttgtctaaa tgcagacttt accatttcca gcagtccccg
     1141 ggagtccggg acatttccgc tttcgacttg aggacccatc aacagggcga aataatcctg
     1201 agtcacattg ggtgaaagcc acttcgaagc ttcagccttt acctcctcca gtgtaattcc
     1261 gggcaaaaat tccttatata atctatattc attttcaata ccaggcagtg gcccaccatt
     1321 gagataattt gagatgaaat tttgaatcaa tacggatgat tcagtagtat ttcgttcatt
     1381 ataacgattt tctatactgc tcatcgtgct cttctttgcc aattcaaatt ctgatgcatt
     1441 gaagccatat ttctgagccc gcaggaattc tgcaacagaa gcatcaatcg ttcccttaat
     1501 atcagaggag ggcatggcca tcaataaaag ctgttcgtac ctctctgtaa aaatgcctcc
     1561 tacatcagaa ctggcaaaaa tgtaaggggg attagcgcta tgtgcaagat cagaatatcg
     1621 tttattcagc atttgagtaa aaagggaacg cacaattcga gttctgtagt catttagtgt
     1681 cacttcaggt ttgacacgac ttcctgaaaa agctaatata aattgataac tggtagcttc
     1741 cttatcagta acaaccattg ctttggcctc cttaaacgga tttatttcag catattctct
     1801 cgtacgagga tttttaggcc ctttgatggc tgaaaaatga tttttgatca tctgttcagc
     1861 tttctccttt gttatgtcac ccaccacagc cactgccatt aaatccgggc ggtaccaatc
     1921 acgataaaat ctccttaatt cctcatattt gaaagtttta aggatttcgt ccttgcctat
     1981 gggtaatctg gatgaatact tagagcccga taataattca ggtaaaaact gctgcatcat
     2041 acgttcattg gcgttttttc ctcttgcccg actctcctca agcactaccc ctctttcatc
     2101 gtcaatatcc ttatccaaca ataaggcatt atgagcccaa tcttccaaaa tctggaagcc
     2161 tttctccaaa ttaccgggct tatcggtcgg gataggcaga atgtaaactg tctggtcaaa
     2221 accggtatat gcattcagat cggctccaaa ttgcactccg atggattgca ggaaactgac
     2281 ctattggttc ttttcaaaat tcttagttcc attaaaattc atgtgttcca taaagtgtgc
     2341 caaaccttgc tgactatcat cttcaagtat cgatccggca ttgatgacca atcggagttc
     2401 taccttattt tcaggttttg agtttggcat aatgtagtaa gtaagtccat tagatagctt
     2461 cccaatcgtc aagttcttat caatgggaat cggtttggag agatcctggg catcaatccg
     2521 ggatattgag cccaacatga ataataccat aacaggaagg aagaacttta caaattgttt
     2581 tttcatattg aaaaccgtgt ttagtatgaa gagaattatt aaaaagttgc gtaaaaatcc
     2641 agccacaaag attataaagt attcattaag gtgaaaggtc aattcttatg atgactggta
     2701 tgcaaatata tactttctaa cggaatgggc aggaggaatg aaagaataat tgtaatatat
     2761 ccctgattga tataatttta catttcattt ttttaattgt tggatgaaac attttggact
     2821 gattggcaag agcctaaaac attccttctc gaaaaagtat tttgatgaga aattcaaggc
     2881 attcggtttg cttgattttg attttagact gattgaaatt ccggatatcg aagatttacc
     2941 tgaaaaggtc aaggagtatg aactatccgg attttccgta acgataccct ataaagaagc
     3001 tataataccc ttttgtcact ccttaagtcc tgaagctgaa aagatatgtg cagtcaactg
     3061 tgtgaaagta actgacaatg gatgggttgg ttataataca gattatgtgg gcttcaggga
     3121 cacccttcct ttagatgacc tggcaaaggg aggcactgct atagtttttg ggacaggagg
     3181 tgcatccaga gcaatcatac atgtactttc acgattggga ctggagatta tcaacgtctc
     3241 cacctcatcg aagaaaggga ccattcacta ccaggcccta aaggatcaga tcatcaaaaa
     3301 ggctcttatc ctggtcaatt gtactcccct gggaaccttt cccgaagtag acaaaaatgt
     3361 tccaatcaat ttttctgcaa tacataaagg tcagatctgt tatgatctgg tgtacaatcc
     3421 cgaccagaca aagtttctca gactatcggc acagcaagga gccaggacaa ttaatggcat
     3481 gaaaatgttg attattcagg ctgaagaagc atggaagatc tggaataact gaaaatcata
     3541 atactatagt gatttcaact atttacctaa gcagatttaa cgaaccaacg taacatcacc
     3601 cttgatgatc tgaatacgtc cattcaggaa ttcaacctcg gcaaaatatg cataaacatc
     3661 cggggtacat tcttttcctt taaaagtacc atcccaacct attgaatcat cccccggtgt
     3721 caattccttt ccttcatata cacatgctcc ccatctgttg aatacaagca agcgtttgat
     3781 aaacttagcc ccccgattgg ggaatactgt aaagtagtca ttgattccat cataattagg
     3841 gctaaataca ttgggcgcca caacatcctt actatccaca acccgtatct ccaaagagtc
     3901 agtagaaatg caacctttct gatcttgtgc agtaaccgta aagatataat tccctaccgg
     3961 aacaagggtt acctccctac aattaaaaca attggtaatg agatcggatg gaaaccattc
     4021 aaatattgtc gatccaacag cattctcagt tgttgcagtc aatacaatcg gatcacccaa
     4081 cttaacttca ttgtatggtt gtacagccat agagactttc agtggatcaa tgcaacactt
     4141 agcaataaaa tttttggtaa aggtatgtac acatccatct tgtgaagtga tagtcaatga
     4201 aacagactta tagcccggac catcgtaaat gacacccgga ggattttgct gtgtcgaact
     4261 tccaatattg gaattggccc caaattccca ttgataagaa acaattgggt ccgtcggagc
     4321 aatggaggtg ttgataaa
//
LOCUS       DAOQMC010000197         4322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9865_length_4322_cov_6.466839, whole genome shotgun sequence.
ACCESSION   DAOQMC010000197 DAOQMC010000000
VERSION     DAOQMC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9865_length_4322_cov_6.466839"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKV31_07920"
     CDS             <1..243
                     /locus_tag="PKV31_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92823.1"
                     /translation="ADITQFDLDTLKKKYSKEEKQDLMHLAVCTLLEPEGYYSYKGLD
                     DDGWPHWDIVKPFTLTGVDAQERYLKELIVKYMDSL"
     gene            complement(238..1473)
                     /locus_tag="PKV31_07925"
     CDS             complement(238..1473)
                     /locus_tag="PKV31_07925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB92824.1"
                     /translation="MKFDTLDIDERLLEGIDAIGFTDMTPVQEQVIPVILEGRDVIAS
                     AQTGTGKTAAFLLPVIHKLLTNDHDESKINCIIIVPTRELALQIVQTLDGLSYFTDIS
                     SIAVYGGGDGPSFANEKNALSSGVDMVVCTPGRMIAHLNMGHVDLSDVRYLVLDEADR
                     MLDMGFSDDIKKIISKLPQKRQSLMFSATMPHKIRELARTLLSDPFELNIAISKPPEK
                     ITQEAFIVYEAQKMPLIRMVLKSKDFGSIIVFCSKKQHVKVLTHELKRAGFSVDEVHS
                     DLEQTKREQTLLDFKNKKLKILVATDVLSRGIDIEDIDLVVNYDVPNDGEDYVHRIGR
                     TARAASDGTAYTFVSEKEQRKFHEIERLLGKEVTKAVIPEKLGPTPSYNPILAKRSNK
                     KRNFRPRNRRKPGPGQKSS"
     gene            complement(1542..2231)
                     /locus_tag="PKV31_07930"
     CDS             complement(1542..2231)
                     /locus_tag="PKV31_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB92825.1"
                     /translation="MEEVKPYFKFGLSIDCVIFGYDKGALKILLIKRKEAPFFNTWAL
                     PGNLVNPDEELESAAVRILKEITGLESIYLEQVRTFGEVNRHPIGRVITTAYYSLINI
                     NNFNLVPGSLAERAEWVDIKHVKQLAFDHNNIFNACFNHLKNSIRTKPIGFELLPQKF
                     TLSQLQGLYEAILQQPLDKRNFRKKILSMNILIDNKEQQRHVAHRPAKLFEFDEDKYN
                     NFLREGFTFDL"
     gene            complement(2568..3701)
                     /gene="chrA"
                     /locus_tag="PKV31_07935"
     CDS             complement(2568..3701)
                     /gene="chrA"
                     /locus_tag="PKV31_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNB92826.1"
                     /translation="MATLFDIFWFFLKAGAFSFGGPNSHIKLMHNELVTKKHLIGEEE
                     FIKIKRLCNTLPGPPSMMLAMKIGYIRKGVLGQLAALIGFILPSSLLMLVIAIYYKEF
                     VNDNFKIIDPFLYGMRTSVIAILLYTTLVYCIRYIRDFKLGAIFLVVAGAALAGLNIV
                     LLLFLSGAAYVLLRQFSSVKKSSLMLILQAQPQFVLPVLDNQKIFWVFVKLGSLILGS
                     GYFVFALIKMDLVDNGLFSINALLDTVAFSLFVPGSIMVAVTFVGYIISSWQGAALAL
                     GGALVPIFVYSLLSSTLLSRLISTASYKLFFRGVLVASLAVLLSIIFQLGYETIGDIR
                     QWVLFILCMLIMFKWPKLNLLWIIIIGANLGYIILHFYPLAID"
     gene            3977..>4322
                     /locus_tag="PKV31_07940"
     CDS             3977..>4322
                     /locus_tag="PKV31_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HNB92827.1"
                     /translation="MRTKFPDFTNTEIAFATKSDAELKKMHWLFDKMNRPWLVDLGTR
                     FGLFAIRVNFPFAQTILKNTLFEQFCGGRTLLECVSNVEKLASKNVMTILDYGAEGKD
                     TEDDFNLTMNQII"
BASE COUNT         1327 a          935 c          813 g         1247 t
ORIGIN      
        1 gcagacatta cccagtttga cttagatacg ctgaagaaaa aatacagtaa ggaggaaaaa
       61 caggatttga tgcaccttgc cgtatgtaca cttctggaac cggaaggata ttattcctac
      121 aaaggcctgg atgatgatgg ctggcctcac tgggatattg taaaaccatt taccctgacc
      181 ggagtggatg cacaggagcg ctacctgaaa gaattgatcg tcaaatacat ggattcccta
      241 tgatgacttt tgtcccggac ctggttttct tcgatttcta ggcctgaaat ttcgcttttt
      301 attgctcctt ttcgcaagaa ttggattgta ggatggggtc ggacctaatt tttcaggtat
      361 gactgccttt gtaacctcct tgccaagtag tctctctatt tcgtggaact tccgttgttc
      421 cttttccgat acaaaggtat aggccgtgcc atctgatgct gcacgtgcag ttcgtccaat
      481 acggtgcaca tagtcttcac catcattagg cacatcataa ttgaccacca aatcgatatc
      541 ctcaatatcg atccccctgc tcaacacatc ggtggcgaca aggatcttga gcttcttatt
      601 cttgaaatca aggagggttt gttctcgttt tgtttgttcc agatcactgt ggacttcatc
      661 gacagaaaat cccgcccgct tcagttcatg tgtcagcacc tttacatgtt gctttttact
      721 gcaaaaaaca atgatactgc caaaatcttt tgacttaaga accatcctga tcaatggcat
      781 tttctgtgcc tcatacacaa taaatgcttc ctgcgtaatt ttttccggcg gcttggatat
      841 ggcaatattc agttcaaaag gatcggacaa taaggttcgg gcaagttccc tgatcttatg
      901 tggcatggta gcggaaaaca taaggctttg cctcttttgg ggtaacttgg agataatctt
      961 tttgatatca tcactaaagc ccatatcaag catgcgatca gcctcatcca gcacaagata
     1021 ccggacatca ctcagatcga catgacccat gtttaggtga gcaatcatcc taccgggcgt
     1081 acatacaacc atatccacgc ccgaactcaa tgcatttttt tcattggcaa atgaaggtcc
     1141 atcaccgcca ccatatactg caatactgct gatatccgta aagtaagaca gcccatccag
     1201 tgtctggacg atttgtagtg ccaattcacg ggttggtaca atgatgatgc aattgatctt
     1261 gctttcatcg tgatcatttg taagtaattt atggatgacg ggcagcagga aggccgctgt
     1321 cttccccgta ccggtctggg cagaagcgat cacatcacgt ccttcgagaa tcacaggaat
     1381 cacttgttcc tgaacaggcg tcatatccgt gaatccgatg gcatcaattc cttcgagcaa
     1441 ccgctcatca atatccagag tatcaaattt caaatccagt cattttaaat ccgttagcgg
     1501 aattaaagca ttgccttatc aggtagagca gagcttgtgt atcaaagatc aaaggtaaac
     1561 ccttctctga gaaaattatt gtatttatcc tcatcaaatt caaatagttt ggccggacga
     1621 tgcgctacat gcctttgttg ttccttatta tcgataagga tattcatcga aagtatcttt
     1681 tttctgaaat tacgtttgtc caatggctgc tgcaatattg cctcatagag tccttgcagt
     1741 tgagaaaggg taaatttttg aggcaacaac tcaaatccaa taggcttggt cctgatactg
     1801 ttctttagat gattgaagca tgcattgaaa atattattat gatcgaatgc cagttgcttt
     1861 acgtgtttga tatcgaccca ttctgcacgc tccgccagag atccaggcac tagattgaaa
     1921 ttattgatat tgatgaggga gtaataggct gttgtgataa ccctcccaat tggatgacgg
     1981 ttgacctcgc caaatgtcct cacctgttca aggtagatcg attccagtcc ggtaatctcc
     2041 ttcaaaatcc tcaccgcagc cgactccagt tcttcgtcag ggttgaccag gttgcccggt
     2101 aatgcccatg tattgaaaaa cggcgcttcc tttcgcttga tcagtaatat cttcaatgct
     2161 cccttgtcat atccgaatat tacgcagtct attgataagc caaacttaaa atatggtttc
     2221 acttcttcca tttaataaat atttcatctt ttaatttaca tatcatacac gaaatatcct
     2281 cccatcatcg aagactatac aatgccacct aaatatgaaa aggcagtacc ctttagctct
     2341 caaaaatgat aacttcggaa tgataaatcg cacaaagaat ctcaattatt ctttatactt
     2401 ggtgtaaaaa taacaataag tataaattat ttaacaaaga ataaaatagt tttataaaat
     2461 attatgttaa aaccgtccaa agatagcata tattaagcat tgaccggagt attaatactg
     2521 ctaaagtagt aggaatcagc cgccggatgg tcgtaatcac cgccacatca gtcaatggca
     2581 agtgggtaga aatgcagtat gatgtaaccc agattggctc ctatgataat tatccacagc
     2641 aaattcaatt tcggccattt aaacatgata agcatgcata aaatgaacaa aacccactga
     2701 cgtatgtctc caatcgtttc ataccccagc tgaaatataa tggataacaa tactgccaga
     2761 gaggccacca gaacacctct gaaaaataat ttataggaag ccgttgatat caaacgcgac
     2821 aaaagggtcg aactaagcag ggaataaaca aaaatcggaa ccaatgcacc gcccaatgcc
     2881 agagcagctc cctgccaact actgataata taacccacaa aggtaactgc gaccataatg
     2941 gatcccggca caaacaagct gaaagccaca gtgtccaaca aggcattgat agagaagagg
     3001 ccattgtcca ccagatccat tttgatcaat gcaaatacaa aataaccact tccgaggatc
     3061 aatgaaccta acttgacaaa tacccagaat atcttttgat tatcaagtac aggaagcacg
     3121 aactgtggtt gagcctgcaa aattagcatc agggatgatt ttttcacact tgaaaattgc
     3181 cttaacaaaa cataggcagc tccactgaga aacaataaaa gcacgatgtt cagaccagcc
     3241 aaagccgcac cggccactac aagaaatatt gcacccaact taaaatcacg aatataacgg
     3301 atacaatata ccagtgttgt atagagcaat atggctatta cagaagtcct catcccatac
     3361 aggaaaggat caataatctt aaagttatca ttgacgaatt ccttatagta aatggcaatc
     3421 accagcatca gtaaagaaga tggtaatata aaacctatca aggctgccag ttgcccgagt
     3481 acacctttgc ggatgtaccc aatcttcatg gcaagcatca tacttggggg ccctggtaat
     3541 gtgttgcaca gtcttttgat tttgataaat tcttcctcac ctatgagatg cttctttgta
     3601 actaattcat tgtgcatcag cttaatgtga gaattcggcc cgccaaagct gaatgcaccg
     3661 gcttttagaa aaaaccaaaa tatatcaaaa agtgttgcca tctatcgctt cttgttgcaa
     3721 atatataaaa atatttttaa tatgataggg tcccagccat tttcaagtca cctgagtgtg
     3781 catttaatag atttttggct gatcatttat cagatttcaa gataatatgg cttctttctt
     3841 ctatcctccg tcattaacag agctttattt atagataaaa aaattgagtt tcatgattgg
     3901 ttgatgattt atcccaaatt aatcattgaa tttgcccgaa tccattatat ttgattccaa
     3961 atatcgaaat cgaataatgc gtactaaatt tcctgatttt accaatacag aaatagcttt
     4021 cgccaccaag agtgatgccg agttgaaaaa aatgcattgg ctttttgaca aaatgaatag
     4081 accctggttg gttgaccttg gtaccagatt cggcttgttt gcaatcaggg taaatttccc
     4141 ttttgctcag accattctta agaatacttt gtttgaacag ttttgtggtg gcaggactct
     4201 tcttgagtgt gtgtctaatg tagaaaaact ggcctcaaag aatgtcatga ccatactgga
     4261 ttatggcgct gaaggaaagg ataccgaaga tgatttcaac ctcaccatga atcaaatcat
     4321 tc
//
LOCUS       DAOQMC010000198         4300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9960_length_4300_cov_4.179034, whole genome shotgun sequence.
ACCESSION   DAOQMC010000198 DAOQMC010000000
VERSION     DAOQMC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9960_length_4300_cov_4.179034"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKV31_07945"
     CDS             <1..229
                     /locus_tag="PKV31_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92828.1"
                     /translation="EVESIFLTAGGYGLQSVLLLSLVYGVCSILSIMLMIYLVYKGSK
                     IFANNEFIEHNEKRITGLVLIIVGLLSFFLH"
     gene            239..1099
                     /locus_tag="PKV31_07950"
     CDS             239..1099
                     /locus_tag="PKV31_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNB92829.1"
                     /translation="MKRSVLFLFSFFSFLHLYGQKASIHGADVSTSDTLGITSFNIRY
                     GEANDGINSWPNRKSLIKNYVQSTRPDILGVQEALIGQIAYLDSIPGYEFGGVGRDNG
                     MTEGEYCAIFYNADELEVRESGTFWLSETPGVPTKGWDGACPRICTWAKFKNKNGKEF
                     CVFNTHLDHMGRKARMEGARLIISKINLLNPRIPVFLIGDFNATSEDLPIRIISDFLA
                     DSREISQFPDTTDGFTFNGFKEEGQATERIDYIFVNNFVRVISFHTNQLRPDNKYLSD
                     HYPIEGTFIY"
     gene            complement(1489..2826)
                     /locus_tag="PKV31_07955"
     CDS             complement(1489..2826)
                     /locus_tag="PKV31_07955"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB92830.1"
                     /translation="MGSVIIRNARIVNEGTIIEGDVKVINGYFEQIGRIRHEGDTPEI
                     DFEGDYLIPGIIDDQVHFRQPGLTHKADIRTESMAAAAGGVTTFMEMPNTYPPATTLE
                     LLEDKYSIAEEASIVNYSFFLGASNNNLDEILKADYSQICGVKIFMGSSTGDLLVDNE
                     TALENIFENCPALIATHCEDEATIRQNMEEYIAKYGDNLDAHFHPLIRSAEGCYLSSS
                     RAINLAKKHNSRLHILHISTGKETELFGNDVPLKNKRITAEACVHHLYFSDRDYTRLG
                     NKIKCNPAIKTQEDREAIFKALKSGKLDIVATDHAPHTLDEKNQPYLKAPSGLPLVQH
                     SLNIMLSFVRKEKATLEEVVHWMCHAPAICFRIKGRGYIREGYHADFVRLNLSTNTLV
                     DEESLFYKCGWSPLEGESLPGKVISTWVNGVKVYEDGIIITNQGKRVEFEVDY"
     gene            complement(2922..3854)
                     /gene="mdh"
                     /locus_tag="PKV31_07960"
     CDS             complement(2922..3854)
                     /gene="mdh"
                     /locus_tag="PKV31_07960"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNB92831.1"
                     /translation="MSKVTVVGAGNVGATVANVLAHGDFVNEVVLLDIKGEMAQGKAL
                     DSWQQAPIDGYSTFLIGSSDYADTANSDVVVITAGLPRKPGMSRDDLISTNAGIVNGV
                     TKSILQYSPNAIIIVVSNPLDVMTYAAHKTSGLSSSRVIGMAGILDTARYRAFLAEEL
                     KVSPKDIQAILMGGHGDTMVPLPRYTTVGGIPVTELVAEEKLNAIVERTKKGGGELVQ
                     LMGTSAWYAPGAAAAQMVTAILKDERRIFPCCIKVEGHYGIDGVYVGVPVVLGRNGVE
                     KVIELDLNESEKALLAESTAAVKSVMDVYDGMGL"
     gene            4002..>4300
                     /locus_tag="PKV31_07965"
     CDS             4002..>4300
                     /locus_tag="PKV31_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92832.1"
                     /translation="MRIFLTTSWLSLFFIFCHTLEAQQVRGYCGTQSSPVADAQLDAN
                     IAYLAAHPDVLNTRGITYVPVRFTIVRSASGNGGVNPKSVFRMFCKLNEDYLDQD"
BASE COUNT         1127 a          873 c          917 g         1383 t
ORIGIN      
        1 tgaagtagaa tccatatttc taactgcagg tggctacggt cttcaaagtg tactgttgtt
       61 gtcgctggtt tatggtgttt gcagtattct ttcgattatg cttatgattt atctggtcta
      121 taagggaagc aagatatttg ccaataatga atttattgaa cacaatgaaa agcgaattac
      181 cggattggta ttgattatcg taggcttatt gtctttcttt ttacattaaa tgataaaaat
      241 gaagcgttct gtcttattct tgttctcatt tttttcattt ctgcaccttt atgggcagaa
      301 agcctccata catggggctg atgtttctac atccgatacg ctcgggatta cttcctttaa
      361 tattcgttat ggtgaggcca atgatgggat caatagttgg cctaatagaa agtcattgat
      421 taaaaattat gtacagtcaa cccgtcctga tatcctggga gtgcaggaag cactgattgg
      481 acaaatagcg tatcttgact caataccggg ttatgaattc ggcggtgttg gccgtgacaa
      541 tggaatgaca gaaggtgaat attgtgccat attctataat gccgatgaat tagaagtcag
      601 agagtcgggg acgttctggt tatctgagac gccgggtgtt cctacaaagg gatgggatgg
      661 tgcgtgcccc cgtatttgta cctgggcgaa atttaaaaat aaaaatggaa aagagttttg
      721 tgtatttaat acgcatctgg accatatggg ccgcaaggcg aggatggaag gagcaagact
      781 gatcatttca aaaattaact tgttgaatcc caggattccg gtcttcctca tcggtgactt
      841 taatgcgact tccgaggatt tgcctatcag aatcatttcg gattttttag ctgacagcag
      901 ggagatttct caatttccgg acaccacgga tggttttacc ttcaatggct ttaaggaaga
      961 aggacaggca acagagcgca ttgattacat cttcgtaaat aattttgttc gtgtgatctc
     1021 tttccatacc aaccaactgc ggccggacaa taaatatctt tctgaccatt atccaattga
     1081 aggtactttt atttattaaa agtttttgga aacagaacat ttattttgat ttagcaagac
     1141 gctattatac tgtaagcatt gtgatttttt gagcatcatt gtcaaggcca actgctaatg
     1201 ctacattttt attctcatgc tccgggtatt tcaaattctg aaatttagga tgcaattaaa
     1261 gtcatccttg ttactattgt gattccgaca atccttcaac agactcttgc aactgttgat
     1321 aaagctattg gggttgtggg tgatgagcag taaggcattg gattgaaata gttcggaggc
     1381 gcaatgatgg gaaataaggc agataatttc aaaagaaatt caggcaattc ccgttgaatt
     1441 tatacggata atttattccc tcaggtgatt ttatattggt atcggatttc aatagtcaac
     1501 ctcaaattct actctctttc cctgattggt tatgatgatg ccatcttcgt atactttgac
     1561 gccatttacc caggttgaga ttactttgcc tggaagagat tcgccttcca aaggtgacca
     1621 tccacatttg tagaacaggg attcttcatc aaccaatgtg tttgtactca ggttgaggcg
     1681 aacaaaatct gcatggtacc cttcgcggat ataccctctt ccttttattc taaagcaaat
     1741 agccggggcg tggcacatcc agtggacaac ttcttccagg gtggcttttt ccttcctgac
     1801 aaaactaagc atgatattaa ggctgtgttg gaccaatgga agtcctgaag gggctttcaa
     1861 atagggctga tttttttcat ccagcgtatg aggggcgtgg tcggtggcca ctatatccag
     1921 ttttcctgac tttaatgctt taaatattgc ctctctgtct tcctgtgttt taatggcggg
     1981 attacatttt attttattgc ctaatcgggt ataatccctg tctgaaaagt aaagatggtg
     2041 tacacaggcc tctgcagtga ttcttttgtt tttgagaggc acatcattgc caaatagctc
     2101 cgtctccttg ccggtactga tgtgcaggat atgcagtcgt gagttgtgtt tctttgcaag
     2161 atttatcgcc ctggaagatg aaaggtagca tccttctgca cttcgaatca atggatgaaa
     2221 atgagcatcc aggttgtcac catattttgc tatgtattcc tccatatttt gtctgatagt
     2281 agcttcatcc tcgcaatgtg ttgctatcaa cgccggacaa ttttcaaaaa tattttccag
     2341 ggcagtttca ttatcgacta aaaggtcgcc ggtactgctt cccataaata ttttgacacc
     2401 acagatttga gagtagtcgg ctttcaggat ttcgtccaaa ttattattac ttgctccgag
     2461 aaaaaaggag taattgacaa tacttgcctc ttcagctatg ctgtatttat cttcaagtaa
     2521 ttccagggtg gttgccggag gataggtatt gggcatttcc atgaaggtgg tgactcctcc
     2581 tgctgcggct gccattgatt ctgtcctgat atcggcttta tgagtcaatc cgggttgtct
     2641 gaagtggact tggtcatcaa tgatacccgg gatgagataa tcaccttcaa agtcaatttc
     2701 aggcgtatca ccttcatgcc ggattctgcc aatttgttca aaatagccat tgatgacttt
     2761 gacatctcct tctataattg tgccttcgtt gactatccgt gcattcctaa tgattaccga
     2821 tcccatacat ttatgcttag tttgcaaaaa taatgtttat aacgcaaaac agccacccaa
     2881 ataagagtgg ctgtcctggc atatgttatc attgtggatt atcaaagacc cattccgtca
     2941 tatacatcca tgactgactt gacagcagct gtcgattcag ccaggagtgc cttctcagac
     3001 tcattaaggt ctaactcgat tactttttca acaccatttc gtcccaacac aaccggaaca
     3061 cctacatata caccatcgat accataatgt ccttctactt tgatgcaaca agggaagatc
     3121 cttctttcat cctttaagat ggcagttacc atttgtgctg ctgccgctcc aggtgcatac
     3181 catgctgaag tgcccatcaa ttgtaccagc tcaccaccgc ctttcttggt cctttctact
     3241 atggcgttta gcttttcttc agctaccagc tcagttaccg gaatacctcc gactgtagtg
     3301 tatcggggta gtggaaccat tgtatcaccg tgaccaccca tgaggatagc ctggatatct
     3361 ttaggagaaa ctttgagttc ttcagccagg aaggcacgat agcgcgctgt gtctaatata
     3421 ccggccattc cgattaccct ggatgaagaa aggccggaag ttttgtgcgc agcataagtc
     3481 ataacatcca atggattgga aacaactatg ataatggcgt ttggcgaata ttgaagaata
     3541 ctttttgtta cgccattcac gatgcctgca ttggttgaga tcaggtcatc acggctcata
     3601 ccgggtttgc gtggaaggcc tgcagtaatc actactacat ccgaattcgc tgtatcggca
     3661 tagtcgcttg agccgatcaa aaaggtgcta tatccatcga tgggtgcttg ttgccaggaa
     3721 tcaagggctt ttccttgtgc catttcacct tttatatcca ataaaacgac ttcattgacg
     3781 aaatcgccat gtgccaatac gttggctaca gtggcaccca cattgccggc gccaactaca
     3841 gttactttac tcattgctaa tattattgat gtttggaatt taatttcagg ctgcaaaagt
     3901 atgactttaa atagtaaata aatagtaaat tttgttttat ttaaaaaggc atttccggtt
     3961 tttgcgtatc ttgcagcttt ataattctaa tctcgttctc tatgcgaata tttttgacaa
     4021 cctcctggtt aagtttgttt tttatttttt gtcatacgct tgaagctcag caggtgcggg
     4081 gctattgtgg gacacaaagc agtcctgtgg ccgatgctca gctggatgct aacattgcct
     4141 atctggccgc acaccctgat gtattgaata ctagaggaat tacttatgtt cccgttcgat
     4201 ttacgatagt cagatctgcc agtggaaatg gtggtgtcaa cccgaaatca gttttcagaa
     4261 tgttctgtaa gttgaatgaa gactatttgg atcaggatat
//
LOCUS       DAOQMC010000199         4295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9983_length_4295_cov_5.007311, whole genome shotgun sequence.
ACCESSION   DAOQMC010000199 DAOQMC010000000
VERSION     DAOQMC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9983_length_4295_cov_5.007311"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2545
                     /locus_tag="PKV31_07970"
     CDS             <1..2545
                     /locus_tag="PKV31_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB92833.1"
                     /translation="ILYWGDGTFQKVGRNNGSGHGISLGGDVKYNIYTYEHTYPSRGT
                     YTISMTDPNRNGGILNVNPPNSDNVPFYLETTFTFLNSTFQGYNNTPTLLQPPIDEGC
                     IDQPFIHNPNAFDIDGDSLAYELIVPMMAKNTPVPNYIFPDQIVAGANNILTLNPITG
                     DIVWNAPKKAGEYNIAIRVNEYRQGVLISSTVRDMQILIINCNNRPPKIETITDTCIV
                     VGQTLTFNVMATDPDSPAQKVRLSALGGPMVIEGNKAEFQAPRGFAPSPVSGTFIWAP
                     DCNAISPSPYSIIFKAEDNQKDSTGLVDLKTVRIKVVGPGPENLTGEPNLNTIVLKWT
                     DPYYCDNARHDYFQGFNIWRRVSSSGFEPDSCNPSMEGKGYTKIAFKQKSSDGIFYHY
                     VDDQVEKGKAYCYRIEAEFAKISPGGFPYNVVRSLPSNEVCLQLNLDVPLITKASVLS
                     TGLDDSVRIEWIKPSVSDFDTLKYPGPYRYVLKHGTGLSPTTYTEVPGASFTSQHFLD
                     PIVTYFTHHSLKTESEPYTYIVDFYSDGSGEKYGTSTSASTVFLNVVGSDKKAIITWA
                     ESVPWSNHTYTIYRKNNLNVFDSIGTTQSRRYEDRNLENGKNYCYKIRTSGSYGIAGL
                     PNPILNFSQEQCATPVDTVPPCSPTLKITNDCMAENPSPSIVNKLTWNNPDEVCDTPG
                     DTKGYRIYVIHDKVDEPELIFETNDPKLISLDYASEEYGLAGCYYIVAFDSVFNESPK
                     SNIVCIENCPSYRLPNAFSPNNDGHNDVFKPYPYRFVNKVEFKVFNRWGNVIFETTNP
                     NINWDGNNRAGNKVPDGTYYYTCKVFETIETMTGIENTIILTGYIELIR"
     gene            2600..3193
                     /locus_tag="PKV31_07975"
     CDS             2600..3193
                     /locus_tag="PKV31_07975"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNB92834.1"
                     /translation="MFTGIIEALGKVISDKREGEKIHYRLTCPFTKELKLGESISHDG
                     VCLTVTDITDKDYAVTAIDTTLKITNIDWKEGKLVNLERAMSANSRFDGHIVQGHVDT
                     VGIVKEILHTEGSVQIEISFDSRFAKNIIDKGSITVNGISLTIVKTSNDSFSIAIIPH
                     TWSHTNLHQLKEGDRVNLEFDVIGKYVAKILSAQGVH"
     gene            complement(3239..>4295)
                     /locus_tag="PKV31_07980"
     CDS             complement(3239..>4295)
                     /locus_tag="PKV31_07980"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNB92835.1"
                     /translation="DHFVSFVISLEVLSVSLFGLIGYLKKSTISIEAAIKYLIMAAVA
                     TAFILFGFALIYSVTGKMDLMGLSESFSNGQIDNMAVTGLALVIVGFGFKMALTPFHL
                     WTPDIYSAAPSPVAAFVATISKGAAFIFLMRLFYSTGGLEHFGVWITFVLVATASMFI
                     GNWLGLRQQNLKRLLAYSSISHLGYLMVGFLAVSHAGIKASVFYLVIYFTSILAAFGM
                     ITYLTGKKGEPTMVEDYRGLFWTKPWLAAFFTLVMLSLAGIPLTAGFMGKFYLLTAGM
                     AYGSLFLLIVLVVSTTIGLYYYLRVVAVMLSRSEAQIGEHIGSNSFVLKAVFAVLFVL
                     ILWMGVYPNTFFSLLGM"
BASE COUNT         1417 a          956 c          838 g         1084 t
ORIGIN      
        1 gattctttat tggggcgacg gaaccttcca aaaagtaggc cgcaataacg gctcaggaca
       61 tgggatttct cttggaggag atgtgaagta caatatttat acttacgaac atacatatcc
      121 ttctcgaggt acgtacacga tcagtatgac tgacccaaac cgcaatggag gcatcctcaa
      181 tgtcaaccct cccaattcag ataatgtccc tttctatctg gaaacaacat ttaccttcct
      241 taattcaacc ttccaggggt ataacaacac gcctacccta ttacagccac ctattgacga
      301 aggctgcatc gaccaacctt tcattcacaa tccaaatgct ttcgatatag atggtgacag
      361 cctggcatat gaactcatcg taccgatgat ggctaaaaat acaccggttc ccaattatat
      421 ctttccggat cagattgttg ccggagctaa taatattctg actctcaatc ctatcacagg
      481 cgacatcgtc tggaacgcgc ccaaaaaagc cggcgaatac aacatagcca taagggtaaa
      541 cgaatacagg caaggagtac ttatatcctc tactgtccgt gatatgcaga ttttgattat
      601 aaattgtaat aacagacccc ccaaaattga aacaattaca gatacatgta tagtcgttgg
      661 ccagacactc accttcaacg tgatggcaac cgatccggac agccctgccc agaaagtcag
      721 actatccgct ttgggaggcc caatggttat agaaggcaat aaagctgaat ttcaagcacc
      781 cagaggattt gctccatcac cggtcagcgg aacatttatt tgggctcctg attgtaatgc
      841 tatcagtcct tcaccctact ccattatttt taaagctgag gataatcaaa aagattcgac
      901 cggcctcgta gacctgaaaa cagtcaggat caaggtcgtg ggaccgggcc ctgaaaatct
      961 cacaggtgag cccaatttga acaccatcgt cctgaaatgg acagatccat attattgtga
     1021 taatgcccgg catgattact tccaggggtt caatatctgg aggcgggtca gcagcagtgg
     1081 ctttgaaccc gatagttgta atccgagtat ggaagggaaa ggttatacca agattgcatt
     1141 caaacaaaaa tcatctgacg gtattttcta tcattatgta gatgatcaag ttgaaaaagg
     1201 gaaggcctat tgctaccgca tcgaagccga attcgcaaag atttctcccg gaggattccc
     1261 ttataacgtt gttcggagtc tgccttccaa cgaagtgtgc ctccagctca atctggatgt
     1321 acccttgata acgaaggctt cggttctttc tacaggattg gacgattcag tccgtataga
     1381 gtggatcaaa ccgtcagtat cagacttcga taccctgaaa taccccggtc cttatcgcta
     1441 tgtccttaaa catggaaccg gtctttctcc taccacctat actgaggttc ccggcgcaag
     1501 ttttacttct caacattttc tggatcctat tgtcacgtat tttacccatc actcattgaa
     1561 gaccgagagt gaaccatata cttatattgt tgatttttat tctgatggtt ccggagagaa
     1621 atatggaaca tcaacttcag cctccacagt atttctcaat gtcgtgggat cggacaaaaa
     1681 ggcgattata acctgggccg aatcagttcc ctggtccaat catacctaca ccatttaccg
     1741 gaaaaataat ctaaatgttt tcgattccat tggaactact caatcaagaa ggtatgaaga
     1801 cagaaacctt gagaatggca aaaattattg ctataagatc aggacttcgg gctcttacgg
     1861 gattgccgga cttccaaacc caattcttaa tttttctcag gaacaatgtg ctactccagt
     1921 cgatactgtg cccccatgta gcccaacact caaaatcacc aatgattgta tggctgaaaa
     1981 tccctctcct tcgattgtca acaaacttac gtggaacaat cctgatgaag tttgcgacac
     2041 accgggcgat accaaaggct accgcatcta cgtgattcac gataaagttg atgaaccgga
     2101 actaatattt gaaacaaatg accccaaact gattagcctg gattatgcaa gtgaagaata
     2161 cggcctggcc ggctgttact acatagtagc ctttgattcc gtatttaatg aaagccctaa
     2221 gagtaacatt gtttgtatag aaaattgtcc aagttatcgg ctcccaaacg ctttttcccc
     2281 aaataatgac ggtcataatg atgtgttcaa accgtatccg tatagatttg tcaataaggt
     2341 tgaatttaag gtgttcaatc gatggggtaa cgtaatcttt gaaacaacaa atcccaatat
     2401 caattgggac ggaaacaacc gagcgggaaa taaggtacct gatggaacct attattatac
     2461 atgcaaagta tttgaaacga ttgaaactat gaccgggatc gaaaatacta ttatccttac
     2521 aggttatatc gaactcatca gataaaatac caaactttac ttcattcaaa attatcaatg
     2581 gtttgaaaaa ttcaaaaaca tgtttacagg tatcatagaa gctcttggca aagttatttc
     2641 agataagaga gaaggcgaaa aaatacatta tcggttaacc tgtcccttca caaaagaatt
     2701 aaagcttggt gaaagcatca gtcatgatgg ggtttgtctt acggtaaccg atattactga
     2761 caaggattac gcagtaacag ccatcgatac aacactgaag ataacgaata tagattggaa
     2821 agagggtaag ctggtaaatc ttgaacgtgc tatgtcagcc aacagtcgat tcgatggcca
     2881 tattgtccag ggccatgtag atacggttgg gatagtcaaa gaaatattgc acacagaggg
     2941 ttctgttcag atcgaaatca gctttgacag cagattcgcc aagaatatta ttgataaagg
     3001 aagtattacg gtaaatggca tcagcttgac catagtcaaa acatccaatg attccttctc
     3061 gattgcaatt atccctcata cctggtcaca caccaaccta catcaactaa aagagggaga
     3121 tcgggtcaat ctggaatttg acgtaatagg aaaatatgta gcaaaaattt tatctgcaca
     3181 aggagtacac taataccttt ttgacagcca acaggacaaa aacgaaagag attcttgatt
     3241 acatgcccag taaactaaag aatgtattcg gatatacgcc catccataga attaaaacaa
     3301 agagaaccgc aaatacagcc tttaaaacaa aagaattact tccgatatgc tcaccaatct
     3361 gtgcttcaga acgtgataac ataacagcca ctacgcgtaa gtaataatac aaaccaatgg
     3421 ttgtagatac caccagaaca atcaataaaa atagtgatcc ataagccata ccggccgtaa
     3481 gaaggtaaaa cttacccata aacccggcag taagtggtat ccccgcaagc gaaagcataa
     3541 ccaatgtaaa aaatgcagct agccagggtt ttgtccagaa caaaccacgg tagtcctcga
     3601 ccatggttgg ttctcctttt ttaccggtca ggtaggtaat cataccaaaa gcagcaagaa
     3661 tactggtaaa gtaaatcacc agatagaata cggaagcttt gataccggca tgagaaaccg
     3721 caagaaaacc gaccatcaga taacccaaat gggaaattga agaataagcc agcaatctct
     3781 ttaaattctg ttgcctcaaa ccaagccagt taccgataaa catcgaagct gttgctacca
     3841 gcacgaatgt aatccataca ccgaaatgct ccaggccacc ggtgctgtaa aatagtcgca
     3901 tcaagaaaat aaaggcagca cctttactga tggttgcaac gaatgctgca acaggtgaag
     3961 gagcagcgga atatatatca ggtgtccaga gatggaaagg agtcaacgcc attttaaagc
     4021 caaatcccac gatgaccagt gccaatcctg taacagccat attatcaatc tgcccattgg
     4081 aaaatgattc tgagagtccc atgagatcca tctttcccgt cacagaataa atcaatgcaa
     4141 atccaaacaa tatgaaagca gttgcaactg cagccataat caggtacttt atagccgctt
     4201 cgatagaaat ggtcgatttc ttaagatacc caatcaatcc aaacaaggaa acactgagaa
     4261 cctctaatga gataacgaat gaaacaaaat ggtca
//
LOCUS       DAOQMC010000200         4294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_9990_length_4294_cov_5.318000, whole genome shotgun sequence.
ACCESSION   DAOQMC010000200 DAOQMC010000000
VERSION     DAOQMC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9990_length_4294_cov_5.318000"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="rpoB"
                     /locus_tag="PKV31_07985"
     CDS             complement(<1..1007)
                     /gene="rpoB"
                     /locus_tag="PKV31_07985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534572.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNB92836.1"
                     /translation="MSSNIASLNSELKRHNFGKIKLSDHQPDLLEIQLKSFKEFFQLE
                     TTPENRINEGLYRVFQENFPISDARNIFVLEFLDYYIDPPRYDIDECMQRGLTYSLPL
                     KAKLRLSCNDEEHVDFQTIVQDVFLGNIPYMTPKGTFVINGAQRVVVSQLHRSPGVFF
                     SQTFHPNGTKIYSARVIPFKGAWMEFATDINTVMYAYIDRKKKFPVTTLLRAIGFDSD
                     KDILEIFGLSEEIPAKKKDLQKAVGRKLAARVLRKWTEDFVDEDTGEVVTIERNEIIL
                     ERDVILEEAHFDLILDAGIDTVILQKEDIEEDYTIIYNTLQKDPASSEIEAVEYIYRQ
                     LR"
     gene            complement(1239..1622)
                     /gene="rplL"
                     /locus_tag="PKV31_07990"
     CDS             complement(1239..1622)
                     /gene="rplL"
                     /locus_tag="PKV31_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNB92837.1"
                     /translation="MADLKSFAEQLVNLTVKEVNELAAILKEEYGIEPAAAAVAVAGP
                     VAGGGGGEAAAAVQTEFDVILKSAGANKLAVVKEVKNLLNLGLKEAKDIVDAAPAPLK
                     NGVSKDEADSIKAALDAAGAEVEIK"
     gene            complement(1720..2247)
                     /gene="rplJ"
                     /locus_tag="PKV31_07995"
     CDS             complement(1720..2247)
                     /gene="rplJ"
                     /locus_tag="PKV31_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNB92838.1"
                     /translation="MNKAQKTQVIEDLKEKFANATFFYITDASTLTVAQVNSFRKLCF
                     EQNVQMQVVKNTLVKKAFEASDESKNFSGLYDSLHGPTALLFCETANIPAKILEKFRK
                     ANSKPILKAAYIDSSVYIGDDQIEVLTKLKSKEELVGEIIGLLQSPAKNVISALKSGG
                     STIAGLLKTLEERAN"
     gene            complement(2269..2961)
                     /gene="rplA"
                     /locus_tag="PKV31_08000"
     CDS             complement(2269..2961)
                     /gene="rplA"
                     /locus_tag="PKV31_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNB92839.1"
                     /translation="MSKLTKKQKASQDKIDANKFYPIEEAMAVVKDANYAKFDASVDV
                     HIKLGVDPRKADQAIRGTVSLPHGTGKTKTVAVFCTPEKEDEAKAAGAEYVGLDELID
                     KVAAGWTDVDVVVAMPGVMAKMGKIARILGPRGLMPNPKTGTVTNEVGAAVSEVKKGK
                     ISFRVDKYGIVHSSVGRISFSPSQLADNANELLQTLNKMKPSSAKGTYMKSITVASTM
                     SPGVKIDTRSIK"
     gene            complement(3056..3499)
                     /gene="rplK"
                     /locus_tag="PKV31_08005"
     CDS             complement(3056..3499)
                     /gene="rplK"
                     /locus_tag="PKV31_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNB92840.1"
                     /translation="MAKEVEYKIKLQVKGGQANPAPPVGPALGAKGVNIMEFCKRFNA
                     ETQDKMGKVCPVLITVFKDKSFEFVVKTAPASVQLMELSGAKKGSAESNRNKVGKVTW
                     DQVRAIAEDKMPDLNAFTVESAMKMVAGTARSLGITVEGTAPWDN"
     gene            complement(3548..4117)
                     /gene="nusG"
                     /locus_tag="PKV31_08010"
     CDS             complement(3548..4117)
                     /gene="nusG"
                     /locus_tag="PKV31_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNB92841.1"
                     /translation="MSETPEITVPSDRKWYSLRVISGKEKKAKEAIDLELLRSGWKDI
                     ITQVVVPTEKVYKIEKGKKVIRERNILGGYILIEAVFKKMTGEIIQTINNIPNVIHFL
                     GGQKPIPMQDAEANRLLGKIDESQDMDEAMLEPFIVGETVKIIDGPFNEFVGDIKEVN
                     DEKKKLKVIVKIFGRGTEVELNFVQVEKK"
     gene            complement(4121..4291)
                     /gene="secE"
                     /locus_tag="PKV31_08015"
     CDS             complement(4121..4291)
                     /gene="secE"
                     /locus_tag="PKV31_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNB92842.1"
                     /translation="MKESYDELVHKVEWPTGVMLVQSTVATIVGLLILSLLVLGMDGL
                     NKTLFDFIYKIK"
BASE COUNT         1147 a          978 c          798 g         1371 t
ORIGIN      
        1 cggagctggc ggtagatata ctcgacagct tcaatttcac tacttgccgg gtccttctgg
       61 agtgtgttgt atatgatggt gtaatcttcc tcaatgtcct ctttctgcaa gatcacagta
      121 tcgattcctg catcaagaat tagatcaaag tgcgcctctt ccaggataac gtctctttcg
      181 aggatgattt catttctttc gatggtgaca acttcgccgg tatcttcatc gacaaagtct
      241 tcggtccatt ttctaaggac acgtgcggcc aattttcggc cgactgcttt ctgcaaatcc
      301 ttcttttttg ccgggatctc ttccgaaaga ccaaaaatct ccaggatatc tttatcggag
      361 tcaaaaccga tggctctaag aagtgtggtt accgggaatt tctttttacg gtcaatgtag
      421 gcgtacatca ccgtattgat atcggtggca aactccatcc aggcaccttt aaaggggatt
      481 acccttgccg aataaatctt cgtaccattc gggtgaaaag tttgactaaa aaacacaccg
      541 ggagagcgat gcagctgcga tactacaacg cgttgagcac cattaattac aaaggtaccc
      601 tttggtgtca tataaggaat attgcccaga aacacatcct gaacaatagt ctggaagtca
      661 acatgttctt cgtcattaca ggacaaacgt aacttagcct taagggggag gctataggtc
      721 aatccccttt gcatacattc gtcgatatcg tatcttggtg gatcaatgta gtaatccaga
      781 aactccaaaa cgaagatatt tctggcatcg ctgattggga aattttcctg aaacactcta
      841 tacaaacctt cattgatacg gttttcagga gtggtttcca gctgaaaaaa ttctttaaaa
      901 gactttagct gaatttcgag taagtcaggc tgatggtctg aaagtttgat ttttccaaaa
      961 ttatgtcttt tcaactcgct attgagagag gctatgtttg agctcattag atcgatctgt
     1021 tttagaatat aatgttatcc gggataacct gggatagatg aaatcaagat aattgggacg
     1081 tcattaagta gttttaattc atatccactt ataggcatag gcttagcaag actgtgcaca
     1141 gtcctactaa gcctaatcaa tatagtaaat cactttattc agcaacacta tgtatgtacc
     1201 caactgcacc gaaatgctgt tggctatgga attaaatatt acttaatctc tacctcagct
     1261 cctgcagcat ccaaagccgc tttgatggag tctgcctcat ccttagaaac accattcttc
     1321 aatggagcag gtgcagcatc aactatgtcc ttagcttctt tcaagcccag gttcaacaag
     1381 tttttcactt ctttaacgac tgcaagtttg tttgcacctg ctgacttcag gataacatca
     1441 aattcagttt gaactgctgc tgctgcttca ccaccgccac cacctgctac aggtccggca
     1501 actgctactg ctgctgcagc tggctcgatg ccatattcct ctttgaggat agctgctaat
     1561 tcattaacct ctttaacagt aaggttaact aattgttctg cgaaagattt taaatccgcc
     1621 attgtaataa atttttaaat tttagtcaat aaatatatta ttgcgtaaag cttggaagcc
     1681 gtattcaata ggtggcatca tgatgccttt ggaaaaagat caattcgctc tttcttccaa
     1741 agttttgagt aaccctgcga tggtagaacc tccggatttg agtgcggaga taacattttt
     1801 cgcaggagac tgcaacaatc cgatgatttc gccaaccaat tcttccttgg atttcagctt
     1861 agtcagaact tcaatctgat catcgccaat atagacggaa gaatcaatat atgcggcctt
     1921 taggattggt ttgctattgg cttttctgaa tttctccaga atcttagcag gaatatttgc
     1981 agtctcgcag aaaagcaatg ctgtaggccc atgaagtgaa tcatacaaac cggaaaagtt
     2041 ctttgactca tcagacgcct cgaatgcttt cttcaccaat gtattcttta ctacctgcat
     2101 ctgaacattc tgttcaaagc atagctttct gaagctattt acctgagcaa ccgtcagtgt
     2161 agaagcatca gtgatgtaaa agaaagtcgc attggcgaat ttctctttca ggtcttcaat
     2221 tacctgtgtc ttttgtgctt tattcatatc tgtcagtgtt gatagtaatt atttaataga
     2281 tcgggtatca attttcacgc ccgggctcat ggttgaagcg acagtgatcg acttcatata
     2341 agttcctttt gcggaagaag gcttcatttt attgagtgtt tgcagtaatt cattggcatt
     2401 atcagccaac tgactcggag agaacgaaat ccttcctaca gaagagtgga cgataccgta
     2461 cttatccaca cggaaggaaa tttttccttt cttcacttca gacactgctg ctccaacttc
     2521 attggttact gtacctgttt tagggttagg catcaagcct ctaggaccaa gaatcctggc
     2581 tattttaccc attttagcca taactcccgg cattgcaacc accacgtcaa catcagtcca
     2641 gccagctgca accttgtcaa ttaattcatc aagaccgaca tattcggcac ctgcagcttt
     2701 agcctcatct tctttttccg gggtacaaaa aactgcaact gttttggttt ttccggttcc
     2761 gtgcggcagg gaaactgtac cgcgaatagc ctggtcggcc tttcttggat ccacgcccaa
     2821 tttgatgtga acatcaaccg atgcatcaaa cttggcatag ttggcatctt ttacgacagc
     2881 cattgcttct tcgatgggat agaacttatt ggcatctatc ttatcttgag aagctttctg
     2941 cttttttgtt aatttagaca tctcaaaatg ttttaaggta tatcgttgga ggaatccaac
     3001 tcccttcagt taataaatat ttacatctga cccatccgga tcgttaattt tcaaatcaat
     3061 tgtcccaggg tgctgtacct tcaacggtaa tgcccagtga cctagctgta ccggcaacca
     3121 tcttcatagc gctttcgacc gtaaatgcat tcaaatccgg catcttgtcc tcagcaatgg
     3181 ctctaacctg atcccaagtc acttttccga ccttatttct gtttgattct gccgaacctt
     3241 tctttgcgcc tgacaattcc atcagctgca cagaggccgg agcggtttta actacaaatt
     3301 caaaggactt atctttaaac accgtaataa gaaccgggca gactttgccc attttatcct
     3361 gggtttctgc attgaatctt ttacagaatt ccatgatatt cacacctttg gcacccaatg
     3421 caggaccaac gggtggagca gggtttgcct ggcctccttt tacctgcaat ttgattttat
     3481 attcaacctc ttttgccatg ataaataatt atttgtattg tctttaataa ttcagttccg
     3541 gatgattcta tttcttctcg acctggacaa agttcaattc aacttcagta cccctgccaa
     3601 atatttttac tataaccttt aacttcttct tttcgtcatt cacctctttg atatctccga
     3661 caaattcgtt gaatgggcca tcgatgatct tgacggtttc acccacgata aacggttcga
     3721 gcattgcttc atccatatcc tgggactcat cgatcttacc gagcagtcga ttagcttcgg
     3781 cgtcctgcat tggaattggt ttctggccgc ccagaaaatg tatgacatta ggtatattat
     3841 tgattgtctg gataatttct ccggtcattt tcttaaatac cgcttcaatc aaaatatagc
     3901 caccaaggat atttctttct ctgatgactt tcttcccttt ttcaatctta tatacctttt
     3961 cggtaggaac aactacctgg gtaataatat ccttccaccc tgagcgcaaa agttccaggt
     4021 cgattgcttc cttagctttt ttttccttac cgctgataac tctgagtgaa taccattttc
     4081 gatcactcgg aaccgttatt tcaggagttt cagacattta ttattttatt ttatagataa
     4141 aatcaaacag tgttttattc aaaccgtcca ttccaagtac aagcagggaa aggatcaata
     4201 atccaacgat ggtagctaca gtactttgaa ccaacataac tccggtcggc cattctactt
     4261 tatgcaccaa ttcatcataa gattctttca agta
//
LOCUS       DAOQMC010000201         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10155_length_4253_cov_4.744879, whole genome shotgun sequence.
ACCESSION   DAOQMC010000201 DAOQMC010000000
VERSION     DAOQMC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10155_length_4253_cov_4.744879"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..3798)
                     /locus_tag="PKV31_08020"
     CDS             complement(298..3798)
                     /locus_tag="PKV31_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92843.1"
                     /translation="MLIRFRKYVSYIIPALLLSISAKAQVMGPLGNLRSIFFHPDSLP
                     LSIPETPVPGTWSFYLQDRSTLVLNDFFEISNNIVRRRIPLPDSLSMETVVLSYRIFS
                     KNLSLPYRLMDTSMILQVWDPTLSERELRYASSPLIPERNTMNYRGAFTRGISLGNTQ
                     SLALNSAFNMQLSGELGDDIKIVGALSDNSIPIQPEGNSQQLQEFDKIYIVLSRKNSS
                     LTVGDFELNRPPGYFLNYFKKLQGVQASTTATLKGNKLSTSGGIAVTKGKFNRFTLTV
                     TEGNQGPYRLPGAEGEAFVIVLAGTERVYLDGIQLVRGFDRDYIIDYNSGEITFTPTR
                     IITKDSRIVVEYEYSNQQYLRSFANLNVRYQTKKWDFNLNAYNERDSKNSDRVRELSA
                     EDRMTLQQAGDSISNKLVNSIREIDPSNTSVVVSYQLNDTIINNQPFSFLVFPWKEGS
                     KRYLADFTDVGQNNGYYDRVNDGINGRVYRFAGIDADGRPKGRYNPVSRLTAPAQRQM
                     FTAGAAWKPDTNTVIRLETALSGQDNNLFSSEDDADNYGLGVHVYAERKNILVSDKRK
                     WTASVGGEYEHTGLQFNPITPYRSQEFNRDWNIASIPLESQQYLRLFLNQNIGTQLRL
                     NYALSGLFQPIHFKGVKQEAALNYKLKKFEFRADASYLKSESINETSAFLRPNVSIRR
                     AFPRLGGLNLIATYQGEYNRRRSVSSDTLLATSFAFHVMRLSAQNTFSKNFSSEIYIQ
                     RRNDYLPFTHDLLLANHALEYGIKSTLKSGLSSVLNLTATVRTFDADRIKPGAKFNSK
                     DTYLGQLDYLFSALKNRLSGNTLFEVSSGQEQKTEFNYLAVQPGTGQYVWNDYNNDSI
                     QQVNEFTTEGFPEMRNYVRVNILTNQFIPTLNTSFNQSFRWQGKSTGKNKTARFFNRF
                     SSQTGYRFDRKIKDTTKKGFWNPFYTNIPDTSLVTYLQNFRHTFFYNRGDSRYEIQLN
                     ANTNNVRNVQITGYERRRTDVYGLVIRYSLGAPYTLLFNYNRSHRFSDSEAEAFKLNN
                     FSIDADEIRPEFNWIIASDYRISLNYRFLKQKNLLAPHYEKAASNDIKLNFDFNQNSL
                     SNLRLSASISMIRFDGIRNSAVEFAMLEGLKNGRNYLWSVNYDLNLKNNVQLSFGYDG
                     RKSEGINVVHTGNASIKAYF"
     gene            complement(4015..>4253)
                     /locus_tag="PKV31_08025"
     CDS             complement(4015..>4253)
                     /locus_tag="PKV31_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNB92844.1"
                     /translation="YRNWNGYGAKIELSPEINIMDAFRILPDPQTNGGLLIAVSPDGL
                     EAAREILTDNGYDAFTEPIGRITAKGEKAITVLA"
BASE COUNT         1177 a          902 c          959 g         1215 t
ORIGIN      
        1 ttctttgacc gttttttaac ataatttgtt gaataatgcg caacttgggt taatgctcat
       61 aatcgtggga ggcctttcta tgactatctt tgaagccaat caaaggttat gtataaaaaa
      121 gttattttat tgtggaattc caatcactaa aaaaatggat ttgtcaatgt cattcaatgt
      181 tatatatgcg gagctgatag acggcagttt ttaagaggtg aaaaaagaaa aaaagaatct
      241 ttaggggagc gacatatcaa gcctcaaagc aacgatcaaa gtgctcaccg ggtgccctca
      301 aaaatatgcc ttgatgcttg cattgccggt atgcaccaca ttgattcctt ctgacttcct
      361 gccatcgtaa ccaaagctca actgcacatt gtttttcaaa ttgagatcgt agttgaccga
      421 ccaaagatag ttgcgtccgt ttttcaagcc ctcgagcatc gcaaattcaa cggcagagtt
      481 tctgattcca tcaaacctga tcatcgaaat agaagcggaa agccggaggt tggataaaga
      541 gttctgattg aagtcaaaat ttagcttaat atcattggaa gcggcttttt catagtgggg
      601 tgcaaggaga ttcttttgtt tcaggaagcg atagttcagt gaaatacgat aatcggatgc
      661 aatgatccag ttgaattcgg gccgaatttc atcagcgtca atcgaaaaat tgttgagctt
      721 gaatgcctcc gcctccgaat cactaaaccg gtgcgacctg ttgtaattga acaacagggt
      781 atatggtgca cccaatgaat atctaatcac caatccatac acatccgtcc ttcgtcgctc
      841 gtatcctgtt atctgtacat tccgaacatt gttagtattg gcattcaact gtatctcata
      901 acgcgaatca ccccgattgt aaaaaaaggt atgtctgaag ttctggagat aggtcaccag
      961 tgatgtatcg ggtatatttg tgtagaatgg attccagaat ccctttttag tggtatcctt
     1021 tatttttcgg tcaaaccggt atcctgtctg ggaagaaaac cgattgaaga agcgggcagt
     1081 cttgttctta ccggtggatt ttccctgcca gcggaagctt tgattgaatg aagtattgag
     1141 cgtggggata aattgattgg taagaatgtt gacacggaca taattccgca tctccggaaa
     1201 gccctcagta gtaaattcat tgacttgttg aatagaatca ttattataat cattccacac
     1261 atactggcct gttccgggtt gtacggcgag ataattgaac tctgtttttt gttcctgacc
     1321 tgagctgact tcaaagagtg tattgccgga tagcctgttt tttaaggcgg aaaacagata
     1381 atcaagttgc cccagatagg tatccttgga gttgaatttg gctcctggtt tgatgcgatc
     1441 agcatcgaac gttctcactg tggccgtaag gttgagcacg gaagaaagac cggacttcag
     1501 ggttgatttg atgccgtatt ccagggcatg atttgccagg agcaagtcat gagtaaatgg
     1561 aagatagtca ttgcgccgct gtatataaat ctcagaactg aaattcttgg aaaaagtatt
     1621 ttgagcagat aatctcatta catgaaaggc aaaggaggtg gcaagtaagg tgtcggaaga
     1681 gacggagcgc cgcctgttgt attcgccctg ataagtagca atcaggttta gtccgcccaa
     1741 tcgggggaag gctctcctga tgcttacatt tggtctgagg aatgctgagg tttcattgat
     1801 actttccgac ttgaggtaac tggcatcagc acggaactca aactttttta atttgtaatt
     1861 gagggcagcc tcttgtttta ctcccttgaa atgtatcggc tgaaacaaac cggacagtgc
     1921 ataattgagt cttaactggg tgccgatgtt ctgattcagg aaaagtcgta aatactgttg
     1981 ggattcaagg ggaatactgg cgatgttcca gtcacgattg aattcctggc ttcgatatgg
     2041 tgtgattgga ttgaattgaa gccctgtatg ctcatattct cctccgacac tcgctgtcca
     2101 tttcctttta tctgatacca gtatgttttt tctttcggca tatacatgaa cgccaagccc
     2161 atagttatct gcatcatctt cgcttgaaaa aagattgttg tcctgccctg acaatgctgt
     2221 ttccaatcga attaccgtat tggtgtccgg cttccacgct gcacccgcag taaacatttg
     2281 ccgctgtgct ggagcagtaa gcctggaaac aggattatat ctcccctttg gcctcccgtc
     2341 tgcatctatg cctgcaaatc tatatacacg gccattgatt ccatcattca cacgatcgta
     2401 ataaccattg ttctgaccga catccgtaaa gtcggccaga tacctcttac taccctcctt
     2461 ccatggaaaa actaaaaagg aaaacggctg attgttgatg atcgtatcat tcagctgata
     2521 tgaaacaacc acacttgtat tggaagggtc tatctcccgg attgaattga ccaatttatt
     2581 cgatatgctg tccccggctt gctggagcgt cattctgtcc tctgcactca attcacggac
     2641 acggtccgaa tttttgctgt cgcgctcgtt ataggcattc aggttaaaat cccatttttt
     2701 ggtctgatag cggacattca ggttggcaaa ggatctcaga tattgctgat tggagtattc
     2761 gtattccacc acaatcctcg aatccttggt gatgatgcgc gttggcgtaa aggttatctc
     2821 accgctgtta taatcaataa tgtaatcccg gtcaaaaccg cgcaccaact ggattccgtc
     2881 cagatacaca cgctctgtac ccgctaaaac aataacaaaa gcctctcctt ccgcgccggg
     2941 aagacgatac gggccctgat taccttccgt aacagttagg gtaaatcgat tgaactttcc
     3001 tttggtaacg gcaataccac cacttgtact caacttgttg cccttgagag tggccgtggt
     3061 ggaagcttgc actccctgga gttttttgaa ataattgagg aagtagccgg gggggcgatt
     3121 gagctcgaag tcacctactg tgagactgct gtttttcctg gaaagcacaa tgtaaatctt
     3181 gtcgaattcc tgcagttgtt gtgaattacc ttccggctga atcggaatag agttgtcgga
     3241 cagagcacct acaatcttaa tatcatcacc caactcaccg gagagttgca tgttgaatgc
     3301 actgttgagt gctaaacttt gggtgttacc aagtgaaatg cctcgggtaa atgctccgcg
     3361 ataattcatg gtgttgcgtt caggaataag aggcgaagag gcataccgca gttctctttc
     3421 agagagggtt gggtcccaga cctgcaatat catcgaagtg tccatcaacc tgtatggaag
     3481 agaaaggttc ttagaaaata tgcgatagct caataccacg gtttccatgg aaaggctgtc
     3541 cggcaaggga attctgcgtc tgacaatatt gttgctgatt tcgaagaaat cgttcaacac
     3601 aagagtcgac ctgtcttgca ggtaaaacga ccatgttccc ggaaccggcg tttctggtat
     3661 cgataaaggc aaactatcag gatgaaaaaa tatggaccga aggttgccca gtgggcccat
     3721 cacttgcgcc tttgccgata tcgacaataa caaggcagga atgatatagg acacatattt
     3781 tctaaaccgt atcaacaagc aaatccggaa taattttaca atcagagtat aaaattaacg
     3841 aataatatca caaagatggt attgcctgat aacaggtgca aggacaaagg gaatatgcat
     3901 ggaattaaag aattgaaaac aatgatgcca tgaaacacaa atccatcctc ctgcatgaaa
     3961 tatcgatacc cggactaaca ctttgtaata agtccggagc atcaaacaag cgttctaagc
     4021 aaggacggtt attgcttttt cacccttagc tgttattctt ccaataggtt ctgtaaaggc
     4081 atcgtaacca ttgtctgtta atatttctct ggcagcctcc aggccgtcgg gagatactgc
     4141 aatgagtaag cctccgttgg tctgtgggtc tggcaggatg cgaaatgcgt ccattatatt
     4201 gatttcaggg ctcaactcga tcttagcgcc ataaccattc cagttacgat agg
//
LOCUS       DAOQMC010000202         4251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10167_length_4251_cov_4.710677, whole genome shotgun sequence.
ACCESSION   DAOQMC010000202 DAOQMC010000000
VERSION     DAOQMC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10167_length_4251_cov_4.710677"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKV31_08030"
     CDS             <1..768
                     /locus_tag="PKV31_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB92845.1"
                     /translation="GIFGFFLYLLLTINGSMIVRSVMEEKTNRIIEVMISTVTPKQLM
                     FGKIIGVGLVGLFQILIWCITTPLFIFLITFLFAGDMDMSGMMATGATGEVVQTQVAN
                     SFMGDIIGQLQAVQWWVLIPTIFLFFLLGYLMYSSMFAAIGSTMGDDQGEGQSLTFLV
                     IMPLLFGFYISMAAISSPNSSMAVWSSIIPLFSPIVMPVRLAFDPPVWQIVTSLISLA
                     LSSWFFAWLSARIYRVGILMYGKKATFKDMVRWFKAK"
     gene            1061..1957
                     /locus_tag="PKV31_08035"
     CDS             1061..1957
                     /locus_tag="PKV31_08035"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNB92846.1"
                     /translation="MRNIHFIVNPISGKGRQTKELNEDFLKSMFPSDQFSITMSIIPH
                     KGATGILTEQAIAAGAETIVACGGDGTINEVARHLVHTDIRLGIIPRGSGNGLASHLA
                     IPKNLLSCLNIIRTGNTTKIDTALAGNFRFFSNCGFGFPTELIRQYELIPQRRLSGYI
                     KAGLSSIHAIKDSAMLKITCNGKIMNARHLFVSNSNKMGYGMTLAPGASLKDGLFNVQ
                     VIGCETLISFGIYGLMVLMGKGDKKKEVTDMLATGVEVEGFKPLQAQIDGEFHQLNEQ
                     KIQIINEPASLNVLIANTSNIP"
     gene            1954..3198
                     /locus_tag="PKV31_08040"
     CDS             1954..3198
                     /locus_tag="PKV31_08040"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNB92847.1"
                     /translation="MKATIIAIGDELLIGQVLDTNSQWIARQCTELNIEIVRKTTVSD
                     GIEPIVAAIQEGVALSDIIIMTGGLGPTSDDYTPRAIAEYFGMEIKFHPPTWERILAY
                     FLSRGREPNEALKAQALLPERITFLVNDMGTAPGMYHHWEDKHLVSFPGVPYEMKHLF
                     TDRFVPIIKPYLGDNHVKSRTILTVGKGETDIATAISDIESNLDSRLSIAYLPDIGRV
                     RVRITGRDTNGEDLNGLINRTGDAIIDRLKDSVYGEGKLTLSEAIRDIMVEKGLTLGL
                     AESCTGGNISRQVVSASGSSAYYKGGIVSYSNQAKQSLLGVSPDSLEQYGAVSEQVAK
                     EMAEGALNVFHSDIAASVTGIAGPDGGSESKPVGTFWVGLAMKDKETRAFKINFNRDR
                     ERNIEFVSIFVMNTIRMSILSM"
     gene            complement(3471..3794)
                     /locus_tag="PKV31_08045"
     CDS             complement(3471..3794)
                     /locus_tag="PKV31_08045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNB92848.1"
                     /translation="MKFSQYEIVLVNLEPTLGSEISKTIPCVVVSPDEMNHYLNKIVI
                     IPITSTLKKYPTRISISNHNVTGMAAVDQIRTIDKRRIVKIIGSVEIETIEKIKKTID
                     ETYVK"
     gene            complement(3791..4030)
                     /locus_tag="PKV31_08050"
     CDS             complement(3791..4030)
                     /locus_tag="PKV31_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNB92849.1"
                     /translation="MLTKIIKIGNSKGLRLSKTILEKYNIGETVNMRMEENYIIIEPN
                     TKPRTDWVKSFKEMRKNGDDELLIQDIFEEESKWV"
BASE COUNT         1241 a          848 c          881 g         1281 t
ORIGIN      
        1 ggtatcttcg gattctttct ttatttacta ttaaccatca atgggtcgat gattgtgaga
       61 agcgtgatgg aagagaagac taaccgaatt atcgaagtta tgatcagcac tgtgactccc
      121 aaacaattga tgttcggaaa gattatcggt gtagggctgg tgggcttgtt tcagatcctg
      181 atatggtgta tcactactcc cctcttcatc tttctgatca ccttcctgtt tgcaggtgat
      241 atggacatgt caggcatgat ggctacgggc gcgaccggag aagttgtcca gacacaggtc
      301 gccaattcct ttatgggaga tattatcggt caattgcagg cagtgcaatg gtgggtactg
      361 attccgacta tttttttgtt tttcctgctg ggttacctga tgtattcttc catgtttgct
      421 gcaataggtt ctactatggg tgatgatcag ggcgaaggcc agagccttac ctttctcgtc
      481 atcatgcctt tgttatttgg tttctatatc tccatggctg ccatcagttc acccaatagc
      541 tcaatggcag tatggagcag catcatacct ttattttcac ccattgtaat gcccgtcaga
      601 ctggcatttg atcctcctgt ttggcagata gtgaccagtc tgatatccct ggcattgtca
      661 tcctggtttt ttgcctggct ttcagcaagg atatatcgtg taggaatatt gatgtacggc
      721 aaaaaggcaa cattcaaaga catggtaaga tggttcaaag ccaaataata aaaggcagac
      781 acttttgcct acctttcatt caatgatatc tattaaccta aagattttca aaaacaatgg
      841 agggaactcg cttaccaacg actggtttca tgaatatcct tgtgaataca tttccactcc
      901 tttatcaaat aacgaaaacg caaatcggta tgctgccatc caggatattt ttatccggaa
      961 tatatcatcg atgatgggcc ctgttttaaa atttttaggt aaattagaga tgaccaatac
     1021 ataaacaaaa tataaagtac ttattgtctg ttgttcttat atgagaaata ttcattttat
     1081 cgtaaatcca atttcaggaa aaggtagaca aacaaaggaa ttgaatgaag actttctgaa
     1141 atcaatgttc ccatctgatc aattctccat cacaatgtca atcattcccc acaaaggagc
     1201 aaccggtatt ctcacagaac aagccattgc tgccggtgct gaaaccatag ttgcatgcgg
     1261 cggggatggt acgatcaatg aagtagccag gcaccttgta cacaccgata tccgcctagg
     1321 catcatacca cgcgggtccg gcaatgggct ggcaagccac ctggcaattc caaagaatct
     1381 gctttcatgc cttaatatca tccgcacagg caatacaaca aaaatagata ctgcattggc
     1441 aggtaacttt cggttcttca gtaactgcgg ttttggcttt cctacagaat taatcaggca
     1501 gtacgaacta attcctcaaa ggcgtttgtc gggatacatc aaggccggat tatcatccat
     1561 tcacgccatc aaggattctg ctatgttgaa gataacttgc aacggtaaga tcatgaatgc
     1621 ccgacatctc tttgtctcca acagtaacaa gatgggatat ggcatgacac ttgcaccggg
     1681 agcatccctt aaggacggat tgtttaatgt acaggttatt gggtgtgaaa cccttatttc
     1741 attcggaatt tatggactaa tggttcttat gggaaaggga gacaagaaaa aagaagtaac
     1801 tgacatgctc gcaaccggag ttgaggtaga gggatttaaa cctttacagg cacagatcga
     1861 tggagaattt catcaattga atgagcaaaa aatccaaatc atcaacgaac cggcatccct
     1921 gaatgtttta atagctaaca catcaaacat accatgaaag caaccatcat agccatcggc
     1981 gatgaattgt tgatcggtca ggttctggac accaacagtc aatggatcgc ccgtcaatgt
     2041 actgaactga acatcgaaat agtaaggaag actacggttt cggacggaat tgaaccaata
     2101 gtagcggcca tacaggaagg agtcgcctta tcagatatta tcatcatgac aggggggctt
     2161 ggtccaacga gtgacgatta tacccccagg gccattgccg aatattttgg gatggaaatc
     2221 aaatttcacc caccgacctg ggaaagaata ctagcctatt ttctgtcaag gggacgtgaa
     2281 cctaacgaag ccttgaaagc ccaggctttg ttgccggaac gaatcacttt tctcgtcaat
     2341 gatatgggta ccgctcctgg aatgtatcac cattgggaag ataagcatct ggtctccttc
     2401 cccggtgtac cctacgaaat gaagcacctt tttaccgacc gattcgtccc catcattaaa
     2461 ccatatttag gtgacaacca tgttaagtcc aggactattc tgaccgttgg caaaggtgaa
     2521 acggacattg ctacagcaat tagtgatatt gaatcaaatc ttgactccag actctccatt
     2581 gcctatttac cggatatcgg gcgtgtgcga gtccggatta caggtaggga tacgaacgga
     2641 gaagacttga atggactcat aaacagaacc ggcgacgcaa tcattgatcg gcttaaggat
     2701 tccgtgtatg gtgaaggtaa acttacccta tcagaggcca tcagggatat aatggtagaa
     2761 aaaggtttga cacttggact ggcagaaagc tgtaccggag ggaatatcag cagacaggtt
     2821 gtttctgcat ccggatcctc cgcatattat aaaggaggaa ttgtatctta ttccaatcag
     2881 gcaaaacaaa gcttgcttgg tgtaagcccg gacagtctgg agcagtatgg tgcggtaagt
     2941 gaacaggttg caaaggagat ggcagaaggt gcactaaatg tatttcattc agatattgcg
     3001 gcaagtgtaa ccggaattgc aggcccggat ggaggaagtg aatccaaacc ggtaggtacg
     3061 ttttgggtcg gcctggcaat gaaagataag gaaacccgcg cattcaagat caatttcaat
     3121 cgggacagag agaggaatat tgaatttgta tccatttttg tgatgaatac aatccgtatg
     3181 tctatccttt ccatgtagga tacaatatcc tatcctaaaa atgccaggat atatccttac
     3241 tccggtaaat acccggacat gtgagttgcc tgaataactt tgacgaaagg gcctggcaga
     3301 acaaaatatc atcgtcatga aagcctgtta aacaccggag ggagtccttt tcctttcacc
     3361 tgcgaaataa ttgctaaaaa gaattaccgg tctttttgaa aattggtaga tttcctgaag
     3421 acaagatgct ttatctaata acgtccagtg cattgttctc accagtatta ttatttaaca
     3481 tatgtttcat cgattgtttt ttttattttt tctattgttt caatttctac gcttcctatg
     3541 attttaacaa ttctccgttt atcaatagtt cttatttgat ccacggccgc cattcccgtt
     3601 acattatgat ttgatatact aatccttgtt ggatatttct taagcgttga ggttattgga
     3661 ataattacaa ttttgttcaa atagtggttc atttcatcgg gtgatactac cacgcaaggt
     3721 attgtcttac ttatttcact tcccaaagtt ggctctaaat taacgaggac aatttcatat
     3781 tgactaaatt tcatacccat tttgattctt cttcgaaaat gtcttggatt aataattcat
     3841 catctccatt ttttcgcatt tctttgaaag attttaccca atcggttcgt ggtttagtat
     3901 tgggttctat tataatgtag ttttcttcca tcctcatatt tacagtttct ccaatattat
     3961 acttctctag aatagtttta cttaatcgaa gtccctttga attgcctatt ttaattattt
     4021 tggttagcat ggtaaatttt tattgcaaag ttattacatt gtaatcacat ttacagattt
     4081 attatattgg tgagaacata tgtttcatgc gttcgtgact cgccaattga tttataaaca
     4141 tctgataatc attataattc ggtgaaaagt gaacatgatt tttctgattg gaagtaaaat
     4201 gacagaatac atgtaaaaag taagtttctg taaaatcctg aaataattaa a
//
LOCUS       DAOQMC010000203         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10174_length_4249_cov_5.285169, whole genome shotgun sequence.
ACCESSION   DAOQMC010000203 DAOQMC010000000
VERSION     DAOQMC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10174_length_4249_cov_5.285169"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKV31_08055"
     CDS             complement(<1..373)
                     /locus_tag="PKV31_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92850.1"
                     /translation="MKKSTYYLILAFVTLILSSCTTTLYVPNAVNTPLLKEKHEFKAT
                     IGPNNAQAAFGLTDNFGIIANGYWDKFKSETTTNGQTTQTLNKGNLYELGLGYYKPLT
                     KVIVFETYLGGGLGNINFNQDN"
     gene            complement(469..1026)
                     /locus_tag="PKV31_08060"
     CDS             complement(469..1026)
                     /locus_tag="PKV31_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNB92851.1"
                     /translation="MEKVKIDKGSILLAEPFMMDSNFIKAAIIITDYHPTGTIGFILN
                     KPINMKINDLLASFPEFESDVFFGGPVATDTIHFLHNLGDLLEGSKYITKGVYWGGNF
                     EKLKFLITSGLVYPNQIRFYVGYSGWSPGQLEEEISTSSWVISESDPNYVFKSNSDGL
                     WKQVMIDKGELYAILSELPENVNLN"
     gene            complement(1161..2039)
                     /gene="fabD"
                     /locus_tag="PKV31_08065"
     CDS             complement(1161..2039)
                     /gene="fabD"
                     /locus_tag="PKV31_08065"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNB92852.1"
                     /translation="MKSYLFPGQGAQFLGMGKDMYDQYDHIARMFEEANKLLGFEITD
                     ILFGDDEDALKRTDVTQPAIYIHSMAKALILGDDFRPDMAAGHSLGEFSALAACGAMT
                     FEDGLRLVSMRAKAMQEACEANPGTMAAVLGLDDQIVEEVCGNIDEVVVPANYNCPGQ
                     LVISGSLDGIKIASEKLLAKGALKVVTLQVGGAFHSPLMADAQAKLEKAIRETEMKMP
                     TCKVYQNVNARPGSTVEEIRQNLIEQLTAPVRWTQTMQNMIADGMTSALEVGGTGSVL
                     RGLLRKVDRSIPSETA"
     gene            complement(2210..2911)
                     /locus_tag="PKV31_08070"
     CDS             complement(2210..2911)
                     /locus_tag="PKV31_08070"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNB92853.1"
                     /translation="MKTERLKIAILSGAGISAESGISTFRDQNGLWENHDIMEVASIT
                     GWIKNPALVLDFYNKRRAQLKQVECNRAHMICALLDNHHDVTIITQNVDDLHERAGSK
                     NIIHLHGELLSARSEFDETIKIRCDGDILPGDLAPDGHQLRPDIVWFGEMVPKLEEAA
                     LIVSNCDILIIVGTSLLVYPAAGLINYAFTAKSIYIVDPNLRPKDIPNFNNQRMQLIN
                     ETAGHGMQLVFDAIT"
     gene            complement(2908..>4249)
                     /locus_tag="PKV31_08075"
     CDS             complement(2908..>4249)
                     /locus_tag="PKV31_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNB92854.1"
                     /translation="GNNGADGLEIANLLSSAGYNVEVGVILIAKKSSDTFRHKLENLF
                     SSIPTIELDVQDLHDFTLPFYDVLIDGILGSGINRELPTELQSLITRINQNKQPVFSI
                     DIASGLSCEGTLPKVAIKPHYTIAMGTIKPAYFLAEGVDHVGILIFARIQSFEECLSQ
                     IKSHEFWINPSSAKKILKPYNRLDHKYIHGIASIIGGSYGMSGCIKLSGEAALRSGCG
                     VVYLHVPYNSVDFLQSSLPEAIIHPDDFKYHIGSFENNMRFTAIGIGPGMGYKIKQSE
                     MFGGLLDDNKTIPMVIDADALTILSGWDDWPRRINCPVILTPHKGEFERLFGKFETQQ
                     QRVDAMRNHSTKYGHVILLKGADTVISGPDGSVYFSNTGNSGMATAGSGDVLTGILTG
                     LLAQGYSPIDTAILGSQIHGLSGNIAEANWGKRSIIAGDLVKNIGLAIQSIENQ"
BASE COUNT         1212 a          878 c          818 g         1341 t
ORIGIN      
        1 cattatcctg attgaaatta atattgccca gaccaccgcc caggtatgtt tcaaatacga
       61 tgacctttgt gaggggtttg taatagccta aacccaattc atacaaattg cctttgttca
      121 aggtctgtgt tgtctgcccg ttggttgtag tctcagattt gaacttatcc caatatccat
      181 tggcaattat gccaaaatta tcggttaagc caaaagctgc ctgggcattg ttagggccaa
      241 ttgtggcttt gaattcatgc ttttctttta gtaatggcgt attgactgcg ttgggtacgt
      301 acagtgttgt cgtacaagat gacaatatga gcgttacaaa ggccagaatt aagtaataag
      361 tagacttttt catggtgaaa tatttaaaat gataagaaat cataacatta tatgatcata
      421 tattaaaagg tgcaattatt ttatcctttt cgatatttgc aaatcctatt aattgagatt
      481 gacattttcc ggcaattcgg ataagatcgc atatagctct cctttatcga tcattacctg
      541 tttccacaaa ccatcagaat tggatttaaa cacataattt gggtcacttt cactgattac
      601 ccacgaagat gtgctgattt cttcttccaa ttgaccggga gaccaacctg aataaccgac
      661 ataaaatcta atctggttgg gatataccag accggaagtt atcaaaaatt taagcttttc
      721 aaaatttccg ccccaataga ctccttttgt aatgtactta cttccttcca gcaaatctcc
      781 caggttgtgc aaaaagtgaa tggtatcagt agcaacggga cctccgaaga atacatccga
      841 ctcgaattca gggaaacttg ccaacaaatc attgattttc atgtttatcg gcttgtttag
      901 aataaatcct atagtgccgg tcggatgata atcagtgata atgattgcgg ccttgatgaa
      961 attggaatcc atcataaatg gctctgccaa aagaattgaa cctttatcta tcttaacctt
     1021 ttccaatgtg ctaatccaat gagattactc aaaatcttta ttgttgtaaa tttagcaaat
     1081 tggattggat taaatatatt aatgatcaat attttaaaat ttatttaatg tcaatcggca
     1141 ttatgccaca ttctttttta tcaggcagtt tcacttggga tgcttctgtc aacttttctg
     1201 agtaatcctc ttagaacaga gcctgtacca ccaacttcca gcgctgaagt catcccgtct
     1261 gcaatcatat tttgcatggt ttgggtccat ctgacaggag cagttaattg ctctatgagg
     1321 ttttgacgaa tttcttcaac ggtacttccc gggcgtgcat taacattctg gtaaaccttg
     1381 caggttggca ttttcatttc tgtttctctg atagcttttt caagttttgc ctgggcgtca
     1441 gccatcaacg gtgagtggaa tgccccacca acctgaagag tgactacctt aagcgcgcct
     1501 tttgccaata atttttcaga agcaattttt ataccatcca gtgatcctga tatgactaat
     1561 tgtcccggac aattataatt ggcgggtaca acgacctcat caatgttacc acatacttcc
     1621 tcaacaattt gatcatccaa accgagtaca gcagccatgg tacccggatt tgcttcacag
     1681 gcttcctgca ttgcttttgc tcgcatggaa acaagtctga gtccatcttc aaaggtcatg
     1741 gccccacaag ctgcaagggc agaaaactct ccaagggaat gtcccgcagc catatctggt
     1801 ctgaaatcat cgcctaatat aagggctttc gccatggaat ggatatagat agccggttga
     1861 gtcacgtccg ttcttttaag ggcgtcctca tcatctccaa ataggatatc ggtaatttca
     1921 aatcctaata acttatttgc ttcttcaaac atgcgtgcaa tatggtcata ttggtcatac
     1981 atatccttgc ccatacctaa gaattgcgca ccctggcctg gaaataaata acttttcatt
     2041 ctaagtagtt gtaaattaat ataataaaat aaatatgata aataattact ttaagtattg
     2101 acttattaaa tctgcgctaa tatcgacatc ataaatcata ttattcgaac attagttaaa
     2161 aaaaatcaag attataccat cgatttaggt aattcggttc tatttttctt taggtgattg
     2221 catcaaatac taattgcatg ccgtgaccgg cagtttcatt tattagttgc atacgttggt
     2281 tgttgaaatt ggggatatct tttggtctca aatttggatc tactatgtaa atgctttttg
     2341 cagtaaaagc ataattgata agacctgccg caggataaac caataatgat gtgccgacga
     2401 taatcaaaat atcgcaattg gaaacaatta atgctgcttc ttctaactta ggtaccattt
     2461 ctccaaacca aactatatca ggccttaatt gatgcccgtc aggtgctaag tccccgggta
     2521 aaatatctcc atcacacctg attttaatcg tctcatcaaa ttcgctacgc gcacttagga
     2581 gttctccatg taaatgaatg atatttttgc tgccggcacg ttcgtgcaag tcatcgacat
     2641 tttgagtgat tatcgttaca tcatggtgat tatcaagtaa tgcgcatatc atgtgggcgc
     2701 ggttacactc aacttgtttt aattgtgcgc gacgtttatt ataaaaatca agtaccagag
     2761 caggattctt tatccagccg gttatgctgg ctacttccat gatatcatga ttttcccaca
     2821 gtccgttttg gtctctgaag gttgatatgc cgctctcagc gcttattcct gctccggaga
     2881 gtatagctat tttcagtcgc tctgttttca ttgattttcg atggattgaa tggcaagtcc
     2941 gatatttttg acaaggtcac ctgcgatgat tgatcgcttt ccccaattgg cctcggcaat
     3001 attgccggac aacccgtgta tttgactacc aagaattgct gtgtctatag ggctgtatcc
     3061 ctgggccaat aacccggtca gaattccggt caggacatcg ccggatccgg cagtggccat
     3121 accgctattg ccggtgttgg aaaagtatac agaaccatcc gggccggata ttaccgtatc
     3181 ggctccttta aggagtataa catgaccata tttggttgaa tgattgcgca ttgcatcaac
     3241 cctctgttgt tgtgtttcaa atttaccaaa caaacgttcg aactcacctt tatgaggagt
     3301 aagaatgaca gggcagttta ttctccgggg ccagtcgtcc cagcctgata gaatggtcaa
     3361 ggcatcagca tctataacca tcggaatggt cttgttgtca tcaagcagtc ccccaaacat
     3421 ttccgattgt tttattttgt agcccatgcc tggtccaatt cctattgctg taaaacgcat
     3481 gttgttttca aaggaaccga tatgatattt aaaatcatca ggatggataa tggcctccgg
     3541 aagggatgat tgcagaaagt caacagagtt gtaaggtaca tggagatata caacaccaca
     3601 ccctgaacgc agggcggcct ctcctgaaag tttaatgcag ccggacatgc cataacttcc
     3661 accgataatt gaagcaatac catgtatgta tttgtgatcc agtctattat atggtttcaa
     3721 tattttttta gcactggagg gatttatcca aaattcatga gatttgatct ggctaagaca
     3781 ttcctcgaac gattgaatcc tggcgaagat caatatgccg acatgatcta cgccttcagc
     3841 cagaaaatag gcgggcttta ttgttcccat ggcgatggta taatgaggct ttatcgcaac
     3901 cttgggtaaa gtaccttcac agcttaaacc actggcaata tcaatggaaa aaacaggttg
     3961 tttattttga tttattctgg taataagtga ttgtaattca gttggaagtt ctctgttaat
     4021 acctgaacct aagattccgt ctatcaaaac atcgtaaaag ggtagagtga aatcatgtag
     4081 gtcctgtaca tcaagttcaa tggtaggaat tgaactgaac aggttttcga gcttatgcct
     4141 gaatgtatcc gatgattttt tagcaataag gattacaccg acttcaacat tataccctgc
     4201 actggatagt aagttggcaa tttcaagacc atcagcacca ttatttccg
//
LOCUS       DAOQMC010000204         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10197_length_4242_cov_4.036064, whole genome shotgun sequence.
ACCESSION   DAOQMC010000204 DAOQMC010000000
VERSION     DAOQMC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10197_length_4242_cov_4.036064"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKV31_08080"
     CDS             <1..214
                     /locus_tag="PKV31_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92855.1"
                     /translation="TIAEQLYRKGVKEGKEQGVQEGLKKGLKQGIEKAKFEIIVKLFE
                     NGASIGFITNITGLPENKIKEILNIQ"
     gene            complement(897..2429)
                     /gene="dnaB"
                     /locus_tag="PKV31_08085"
     CDS             complement(897..2429)
                     /gene="dnaB"
                     /locus_tag="PKV31_08085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNB92856.1"
                     /translation="MPNPKQSSSDPVSNLRALKGSNDHNGTIGGKVIPQAVPVEEVIL
                     GAIMIDVNAMSAVLDMLSPEVFYNPAHAKIFAAMMRIFNKSQPIDLLTVNEELKKSGE
                     IDEIGGTAYLAELTFRVGNSANIEHHARIVVQKYIQRELINVSNQIIQDSYEDTKDVF
                     DILDEAEQNLYRITDNNLNNGPQTFTAMANQVRLRMEEMASRPEGLTGVSTGFRELDK
                     LTSGWQPSDLVIIAARPSVGKTALVLSMAVNAAKKGFPVAIFSLEMSTLQMAQRIIAM
                     EAEISSSALRVGKLSDDELKRFHHTLQTKSDLKIFVDDTPAINIFEMRAKCRRLKTQH
                     NIQMIVIDYLQLMSAGSESKMGGNRTQEVSVISRALKGLAKELNVPVIALSQLSRALE
                     TRADKRPMLSDLRESGSIEQDADIVGFIYRPDYHNLAEEGGINVAEPEAELLIQKHRN
                     GALGNIKMTFVREYARYQDPDDANHLFGFGSASAISDSGSSGIIRKGSRANDDDFIDA
                     PF"
     gene            3007..>4242
                     /locus_tag="PKV31_08090"
     CDS             3007..>4242
                     /locus_tag="PKV31_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNB92857.1"
                     /translation="MEYTPKYKQIKEILQQRIMIIDGAMGTMIQQHKLTENDFRGTRF
                     ADVQKDIKGNNEVLSLIRPDIIEAIHKAYLEAGADIIETNTFSANAISQADYDMESLC
                     YEMNLASARIARKAADEQTSLTPDRPRFVAGAMGPTTKLASMSPDVNDPGFRALNYDE
                     LVAAYGEQARGLIDGGADILLIETITDTLNCKAALYAIDEIFEQRGILMPIMISGTIT
                     DQSGRTLSGQTVEAFYISVSHMPLLSIGLNCALGAKEMRPHIEELANISDCFISAYPN
                     AGLPNEMGEYDQDAHEMGHLIRDFALSGFVNIVGGCCGTTPSHIRAMAEKMKSIPPRR
                     LKEQPAYTKLSGLEPLVFRPDINFVNVGERTNVTGSKKFERLIKKKDYAAALEVAQQQ
                     VEGGAQVLDVNMDEGLLDSK"
BASE COUNT         1189 a          881 c          966 g         1206 t
ORIGIN      
        1 gaccatcgcc gaacaattat atcgcaaagg agtaaaggaa gggaaagaac aaggtgttca
       61 ggaaggcctt aaaaaaggct tgaaacaagg tattgaaaaa gcgaagtttg aaataattgt
      121 aaagttattt gaaaatggtg catctatcgg ttttataacc aacattaccg gacttcccga
      181 aaataaaatt aaagaaattt tgaatataca ataatcggtt tgatggagct tgattgtcat
      241 ttatttattc aaatttctac atctccggca gaaatttttt agatttttaa aataaatcat
      301 tcggagggct ctattattaa aggtagcttt atttatcaaa gtcaaaattt aattcctccc
      361 gaattggcca atgcaccttc ccggcaaaca attccgtaaa ccttccccat aaaattgaac
      421 tttagattga tgttccagcc ttaattgact ctctgatttt tccatcaaat ttttcgagcg
      481 atgcaaagtt tccggaacgg ttctccctct tatggaagat tatcagcaaa ttaaggattt
      541 ttaaccttgc gtcacccaaa acagttaccc gactccattc ataatattga ttattaggtc
      601 tttataaaaa tttgtaaatt aaaagtgccg aagtcaggaa aagaatcgga tgataatgcc
      661 ctgtaatagt ttttgcaggg tatttttttt caggtcggag tagaaggcgt atcaaattgg
      721 gaagcagcat acattggtaa agatgaaaag ggccttctat cggtttattt ggcgaacgtg
      781 gcattctcaa tcaagtacaa ttaatgccga ttagcatttt tctcgagtcg gccggacaag
      841 tatctgctcg cggaaggatg gagaatcgtg gtagtcttga aaaatgtaat taatttttaa
      901 aacggagcat ctataaaatc atcatcattc gcccggctgc cttttctgat gatgcccgaa
      961 ctgccggaat cacttattgc tgaagccgaa ccaaatccga ataaatgatt tgcatcgtcc
     1021 gggtcctggt atcgggcgta ttcacgtaca aaggtcatct tgatattgcc aagtgcaccg
     1081 ttacggtgtt tctggatgag gagctctgct tccggttctg cgacattaat gccaccttct
     1141 tctgcgagat tgtgatagtc gggcctgtat atgaatccta cgatatcagc atcctgctct
     1201 atggagcctg attcccgaag gtcggacagc atggggcgct tatcagcccg cgtctccaat
     1261 gcccggctca gctgtgacag ggcgatgacg ggcacattca attctttggc aagacctttt
     1321 aaggcacggg agatgacaga gacttcctgg gtgcggttgc cgcccatctt tgactcactc
     1381 ccggctgaca tcaactgcag gtagtcgatg acgatcatct ggatattgtg ctgtgtcttc
     1441 agccttctgc attttgccct catttcaaag atgttgattg ccggcgtatc gtccacaaag
     1501 atcttcaggt cggactttgt ctgaagggta tgatgaaaac gctttaattc gtcatcagat
     1561 agcttcccga ctcgtaaggc ggagctggat atttcagcct ccatggctat aatccgttga
     1621 gccatctgca gggtggacat ctccaaagag aagatcgcca ccggaaaacc tttcttggct
     1681 gcattgacag ccatggaaag aacaagtgcc gtcttaccca cactgggtcg agctgcgatg
     1741 atgactaaat cggaaggttg ccaccctgaa gtaagcttgt ccagctccct gaaaccggtc
     1801 gacactccgg tcaagccttc aggtcgactg gccatctctt ccattcggag tcttacctga
     1861 ttggccatag cagtaaaggt ctgtggcccg ttgttgaggt tgttgtcggt gatcctgtat
     1921 aggttttgct ctgcctcatc aaggatgtca aatacatctt tggtgtcttc ataactatcc
     1981 tgaatgattt ggttggaaac attgattagt tctcgctgga tatatttctg gaccacgatc
     2041 cgggcatggt gctcaatatt ggccgagttg cccacgcgga atgttagttc cgccagatag
     2101 gccgttccac cgatttcgtc aatttctccg gattttttca actcctcatt gactgttaat
     2161 aagtcgatgg gttgcgattt gttgaaaatg cgcatcatcg cagcaaatat cttcgcatga
     2221 gcgggattgt agaagacttc cggtgacaac atatccagca ccgcggacat ggcgttgaca
     2281 tcaatcatga tggcccccag gatgacttct tccaccggaa ctgcctgagg aattaccttc
     2341 cctccgatgg tgccgttatg gtcgtttgat ccttttaggg cacggagatt agataccgga
     2401 tcggaagaag attgttttgg attaggcata ttgattggat cgtgtgttta agtatgatgc
     2461 aagattaaga aaaaattacc tatctgctta aagaggactc tttcataaga aaacaacact
     2521 gtggataact tagtttccat tgtggaaaat tatgtggata agctgaaatt ttatgtgaat
     2581 acgtcattgc tccgagagat atatttatca attacatata taataatatt ataatataaa
     2641 tatccggccg atgtaaaaac ttgaaaacaa gtctaaaatt attcatatat acaataaaat
     2701 ttatatgtaa attatttaat ataaatattg ctacagaaaa aaccaagtgt tagttttatt
     2761 ttaaaatttt ttattttatg aattccggga ttttcaccgg gcaggtagcc ggcaaccttt
     2821 gggttagaca gggtgaggca aacaaagggt gaggtatttt acttaaggta tctggatgtt
     2881 gacccaatat tatttattta cacgacaatt ttccgaataa tattggcaaa tcaaattctt
     2941 ctcaaccgat atttagataa ttggtgttaa tactgataac tttgcagcat taatttttga
     3001 ccaaaattgg aatacacgcc aaaatataag cagataaagg agattctgca acagcgtatc
     3061 atgatcattg atggtgcaat gggcacgatg atccagcagc acaaattgac tgagaatgac
     3121 tttcgcggca ctcgatttgc cgatgtccaa aaggatatta agggcaataa tgaggtgctc
     3181 agtctaatcc ggcctgacat catagaagcc atacacaaag cctatctcga agccggtgcc
     3241 gatatcattg agaccaatac tttttcggcc aatgctattt ctcaggctga ttatgacatg
     3301 gaatcgttgt gttatgaaat gaatctcgct tcagcgcgaa ttgctcgtaa agccgcagat
     3361 gagcagactt ctctgacccc tgacagaccg cggtttgtgg caggtgcaat gggccctacc
     3421 accaaactag cctctatgtc acctgacgtc aacgatccgg gttttcgggc acttaactat
     3481 gacgaattag tggcggccta tggtgagcag gcacgcggtc tgatcgatgg aggtgcggat
     3541 atcctgttga tagagacaat taccgacacg ctcaattgta aagcagcatt gtatgccatc
     3601 gatgagatat tcgagcagcg cggtatcctg atgcccatca tgatatcggg aacgatcacc
     3661 gaccagagtg gcaggacctt atccggccag accgttgagg ccttctatat atcagtgagt
     3721 cacatgcctt tgttgtctat cggcctcaac tgtgcattgg gagcgaagga aatgcggcct
     3781 catatcgagg aattggcaaa catatcggat tgcttcatat cagcataccc caatgcaggc
     3841 cttcctaatg aaatgggtga atacgatcag gatgctcacg agatgggtca tttgatccgg
     3901 gactttgcgc ttagtggctt tgtcaatatt gtcggaggct gttgtggcac gactccatct
     3961 catattcgcg cgatggcaga aaaaatgaaa tccatcccgc cacgaaggtt aaaggaacaa
     4021 ccggcctata ccaaactcag cggacttgaa ccgcttgtct tccgtcctga tatcaacttt
     4081 gtcaatgtcg gcgaaaggac caatgtcacg ggctcgaaga aatttgagag gctgattaag
     4141 aaaaaagatt atgcagcggc tctggaagta gctcaacagc aggtggaggg cggggcacag
     4201 gtcttggatg tcaatatgga tgaaggtttg ctggatagca aa
//
LOCUS       DAOQMC010000205         4235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10224_length_4235_cov_5.104785, whole genome shotgun sequence.
ACCESSION   DAOQMC010000205 DAOQMC010000000
VERSION     DAOQMC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10224_length_4235_cov_5.104785"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3944
                     /locus_tag="PKV31_08095"
     CDS             <1..3944
                     /locus_tag="PKV31_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNB92858.1"
                     /translation="EVYQYILHPKKGSESKYTASDLRTMQDWIVARQIYGTEGVAEVN
                     SFGGALKQYEVAVDPNRLKAMGVSVSDIFNALEKNNQNTGGAYIDKKPNAYFIRGIGL
                     VTTLEDIRNIAVKDETGSVPIFIKDVADVRFGSAIRYGAMTYNGEKDAVGGVIMMLKG
                     ANSNEVVNSIKAKIPGIQKSLPDDVVIEPFLDRTDLVKRAISTVERNLLEGALIVIFV
                     LVLFLGNLRAGLIVASAIPLSLLFALGMMNIFGVSANLMSLGAIDFGLIVDGAVIIVE
                     AILHLMSLEKTKTVLNSEEMDLVVEASAEKMMNSAAFGQIIILIVYLPILSLTGIEGK
                     MFTPMAMTVSFAILGALILSVTYIPMMSSKFLSRKTTHRETLSDRMMKIFQKFYQPLL
                     QRAFRFKYLVVSAAIGLFLISLLAFRTLGGEFIPQLQEGDFAFHCILPQGSSLTQSIE
                     TSMHASRIIKQFDEVKMVVGKTGSAEVPTDPMPPEVSDLIILLKPQEEWKEQKSYNKL
                     AEEILEKLESIPGIFFEANQPIQMRFNELMTGIRQDVAVKIFGENMDTLLTYAEQSAR
                     VIQQISGVTTPQIEKVSGLPEINVEYDRTRLANYGLNVEDVNDILSTAFAGRVAGQVY
                     EDERRFDLVVRLDTPFRKNIDDVNSLMVPIRGGAQIPLSQIARVSYKLGPAQISRESG
                     KRRIVVGFNVSGRDVESVVSDIQEKLEQQVHLPSGYYFTYGGQFENLQQASKRLLVAV
                     PISLLLIFILLYFTFHSLKQAVLIYTAIPMSAIGGVFALLIRGMPFSISAGIGFIALF
                     GVAVLNGIVLIGTFNQLEKDGIRDLYQRVMEGTRSRLRPVLMTATVASLGFLPMAVSH
                     GAGAEVQKPLATVVIGGLVTATILTLLVLPLLYIIFNTKIKRRMKPDGIVAFVAMLLM
                     SFSSQAQTLVIDNADQAVEWAIKNNPGLRAKNLETLAAHSLIRTSGELPKMDLGIQLG
                     QYSSIRFDNAFSVSQNIPFPTVFKAKREFAESVEAGKDIQAKVSINELRHSVRKMYHS
                     IQCFINIERQLMYLDSLYLEFTEVAKLRHKTGDTKMVDVSTAETKRGEIDLKIKQNTV
                     NMSNAYHNLRVLLNVRDSIRILPEEDYSPLTLSLYTDTLALAHHPTIMAYYLESLILE
                     KSKAVEKAQGLPEFTVGYTNQSLIGNQIIDGQEHFFGAGKRYGYYTAGIAIPLTFGAT
                     KARIQSIEYSRAASEANAIQQQNDLQAQLQSLIRQYEHCLLQYKHYNEKALPNAGEIV
                     RAARLGYRTGDISYMEYLYALQTAADVRFNYIESICQLNQIIIEIYRLLNQ"
     gene            3955..>4235
                     /locus_tag="PKV31_08100"
     CDS             3955..>4235
                     /locus_tag="PKV31_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92859.1"
                     /translation="MKYINSNSINTNPTGDNHSLYFNQKAIKKTFFAFIALLLLLLHA
                     ACQSDTGHISDKAAGDKVEAAAHNHREMQPMVTSLSLEQMKTIGLEFGT"
BASE COUNT         1218 a          836 c         1020 g         1161 t
ORIGIN      
        1 gtgaggtgta tcaatatatc ctgcatccaa agaaaggaag cgaatccaaa tataccgcct
       61 ctgatctcag gaccatgcag gactggatcg tggcacggca aatatacggt acggaaggag
      121 tggcagaggt caacagtttc ggtggcgcac tcaagcaata tgaagttgca gtggacccca
      181 atcggcttaa agcaatggga gtgagtgtct cagatatttt taacgccctg gagaaaaaca
      241 atcagaatac cggtggcgct tatattgaca agaagccgaa tgcatacttt atcagaggca
      301 tagggctggt cactacactg gaggatattc ggaatattgc cgtgaaagat gaaaccggaa
      361 gcgtgcctat ttttattaaa gatgtagcag atgtacgctt cggtagcgct atccgatatg
      421 gtgctatgac ttacaacggt gagaaggatg ccgttggcgg tgtcattatg atgctcaagg
      481 gagccaactc caacgaggtc gtcaatagta tcaaggccaa gattccgggc atacagaagt
      541 ctctgcctga tgatgtcgtc atcgaacctt ttctggaccg aacagacctg gtcaagcgtg
      601 ctataagtac agtggaaaga aacctgcttg aaggtgcctt gattgtgatt tttgtcctgg
      661 tccttttttt gggtaattta agagcgggtt taattgtagc atcggcaatt ccactttccc
      721 ttttatttgc tttgggaatg atgaatatat ttggagtcag tgctaacctg atgagtctgg
      781 gagccattga cttcggtctc attgtggatg gagcagtcat cattgtggaa gccatacttc
      841 atctcatgtc tttggaaaag acaaagacag tacttaatag cgaagaaatg gatcttgtgg
      901 tggaggcaag tgcagagaag atgatgaatt ctgcagcatt cgggcagatt atcatcctta
      961 ttgtctatct gccgatactt tcacttaccg gtattgaagg taagatgttt acgcctatgg
     1021 ctatgaccgt gagctttgcc atcctcggtg ccctcattct ttcggtgact tacattccga
     1081 tgatgagttc caagtttctt tcaagaaaga ctacacatag ggaaacgctt tcggaccgga
     1141 tgatgaagat atttcagaaa ttttatcaac cattgcttca aagggcattt aggtttaaat
     1201 atttggttgt ttcagctgcc attggattgt ttttaatcag cctcctggcg ttcagaacgc
     1261 ttggtgggga atttattccg caacttcagg aaggcgattt tgcatttcat tgcatcctgc
     1321 ctcaggggag ttctttaacc caaagtatcg aaacatccat gcatgcctca cgtatcatta
     1381 agcaattcga tgaagtgaag atggtagttg gtaagacagg ctcggcggaa gtgcctacag
     1441 atccaatgcc gcctgaggtt tccgacctga ttatcctgtt gaagccgcaa gaggaatgga
     1501 aggaacaaaa gtcgtacaac aaattggccg aagaaatcct ggaaaaactg gaaagcattc
     1561 caggaatatt tttcgaagcc aatcagccga tccagatgag gttcaatgag ttgatgacgg
     1621 gtatcaggca ggatgtagcg gttaagattt tcggtgaaaa catggatacg ctactgacct
     1681 atgctgaaca atccgcaagg gttattcagc aaatttcagg tgttacgaca cctcaaatcg
     1741 agaaagtgtc cggtctcccg gagattaatg ttgaatatga tcgcacaaga ttggccaatt
     1801 atggtctgaa tgtggaagat gtaaatgata tactaagtac tgcctttgcg ggaagggttg
     1861 caggccaggt ctatgaagat gaaaggcgct ttgatttagt cgtgagattg gatactccat
     1921 tcagaaaaaa tatcgatgat gtcaacagcc taatggttcc tatcagaggt ggtgctcaaa
     1981 tccctctctc tcaaattgcc cgggtcagct ataagcttgg gcctgcacaa atcagtcggg
     2041 aaagtggcaa acgaaggatt gtagtaggtt ttaatgtttc cggtcgagat gtagaaagtg
     2101 tggtgtctga cattcaggaa aagctggaac agcaagtaca tctgccttcc ggatattatt
     2161 ttacttatgg aggacagttt gaaaatctgc aacaagcgag taaaaggttg ttggtagcag
     2221 taccgatatc cttgttactc atttttatac tgctttattt tacctttcat tccctaaaac
     2281 aggcagtcct gatctataca gcgataccaa tgagtgccat aggcggagtg tttgcattat
     2341 taatacgcgg catgccattc agtatcagtg cagggattgg atttattgcc ctgtttggcg
     2401 ttgcggtgct taatggtatt gtgcttatag gcaccttcaa ccaactggaa aaggatggga
     2461 taagggactt gtatcaaagg gtcatggaag gtaccagatc gaggttgagg ccggtgttga
     2521 tgacggcaac ggtggcttct ctgggattcc ttccaatggc tgtgtctcat ggggctggcg
     2581 ctgaagtaca aaaaccactt gcaactgttg tgataggtgg gctggtgacg gcaaccattc
     2641 tgacattgct ggtgctacca ttgttatata tcattttcaa tactaaaatt aaacggagga
     2701 tgaaacctga cggaatagtc gctttcgtgg caatgttgct aatgagcttt tcatcacagg
     2761 cacagacctt ggtcattgat aatgctgatc aggcagtaga atgggccata aagaataatc
     2821 ccggcttaag agctaaaaat ttagagacat tggctgcaca tagcctgata agaacatccg
     2881 gcgaattgcc caagatggac cttggcatac aattggggca gtatagtagc atcaggtttg
     2941 acaatgcttt cagcgtttct caaaatatac catttccgac agtatttaag gcgaagaggg
     3001 aatttgcaga atctgtcgaa gcaggtaagg atattcaggc aaaggtttcc atcaatgaac
     3061 tccgacatag tgtgcgaaag atgtatcatt ccatccagtg ttttataaac atcgagaggc
     3121 aattgatgta tctggatagc ctttatctgg aatttactga agtggctaag cttcgtcaca
     3181 agacaggtga tacaaaaatg gttgacgtca gtacggctga gaccaagcgg ggtgagattg
     3241 atcttaaaat aaagcagaac acagttaata tgagcaatgc ctatcacaat cttcgtgtgt
     3301 tgctgaatgt cagagactca atccggattc ttccggaaga agactatagc ccactgacac
     3361 tttctctcta taccgacacc ctggcattgg ctcaccatcc gaccataatg gcatactatt
     3421 tggaatcctt aattttggag aagagcaaag cggtggaaaa ggctcaggga cttccggaat
     3481 ttacggtagg ctatactaac caatctctga ttggcaacca gattatcgat ggacaggaac
     3541 atttttttgg agctggtaag aggtatggct attacactgc aggaattgca ataccgctga
     3601 ctttcggtgc cactaaagcc cggattcagt ccattgaata ttccagagcc gcttcggaag
     3661 caaatgccat tcaacagcaa aatgatctgc aggcgcagtt gcaaagtttg atccggcaat
     3721 acgagcattg cctattacaa tacaaacatt ataatgagaa agcattgcca aatgccggcg
     3781 agatcgtaag ggctgcacga ctgggctatc gcaccggtga tataagttat atggaatatt
     3841 tgtatgcttt gcagactgcg gctgatgtac gattcaatta tattgaaagc atctgccaac
     3901 tcaatcagat catcatcgaa atctatagac tcctcaatca ataattttcc tgaaatgaaa
     3961 tatataaatt caaatagtat caatacaaat ccaacaggcg ataatcactc cttatatttt
     4021 aatcaaaagg ccattaagaa gacattcttt gcatttatcg cattgttatt acttctgttg
     4081 catgctgcgt gccaatcaga taccggacat atttccgaca aagctgccgg tgataaggtc
     4141 gaggcggctg cccataacca ccgggaaatg cagcctatgg tcacttcatt gtctttggaa
     4201 caaatgaaaa caatcggact tgaattcgga acctt
//
LOCUS       DAOQMC010000206         4226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10250_length_4226_cov_5.468713, whole genome shotgun sequence.
ACCESSION   DAOQMC010000206 DAOQMC010000000
VERSION     DAOQMC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10250_length_4226_cov_5.468713"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2685)
                     /locus_tag="PKV31_08105"
     CDS             complement(<1..2685)
                     /locus_tag="PKV31_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNB92860.1"
                     /translation="MKTRSGLILLFSLIVFASFAQMTDNGVTKYGNEWIVPGREYYKI
                     RISKDGFYKIDFNALKASGIDGEIVGSQLRLFRNGVEIPITVSANGQWSDSDFLTFHG
                     YYNKSEMDKYSFPNGVNDIVNPKASLYSDTATYFLTINPDGNNLRTNILPNNLINPPS
                     PEPYIIRISEMDFREILNTKTEYVGQHPFVNTPFYPGKGMVTSADNQTYKLSCPGLLP
                     DSRLPIFRYRGITNAGNVGVNLGHDLKFSMKGQQMYSVFSTGQRVMYVNFTYVDTNRV
                     ITASDNQYSLTNGHPLDLIKTGYVAIEYPSDLNIDATRTQSFFLEGKASDRLLDFIAS
                     GDVPTAPYLTDSLGTWRIPGSVTGNTIRFRIPAFTVTSRLIYRPGDDAESISSMTQVK
                     MKDFNNLNADYIILSHNLLMEDNNGPSAVEQYAAYRRSAAGGAYDVEVVNVSDLYNSF
                     GYGLDYNPMAIKNFANFIGLKGSSRFLFIIGRGRDYKEIRSNNQLATAISKGYGVPTF
                     GDFGSDNLLVSKSFHKVDMNLAVGRLPAVTQDEVITYLNKIRASDAAYNAPGDNSSRQ
                     WTKTIVQVNGGNIGKPDQAAISNAQKDVEALIKSSKLAGFVETFVKGSNETIGKASDD
                     FFKLVNQGVAVVDYFGHGALSTLEYPIDLPSKYSNSPRLPILIIKGCKTGNCQRDGSS
                     YPSKLLYEESYKNSGYRAVIGSISDSELYSLSMLAHRFYTLWGGDMYGKTFGELFQAT
                     FNSPGLNNSQEGIQQLYVGDPALMVQPFPGPDFTILPGEVKTIPENIASTDNAFTVQF
                     TVKNLGTSVEDTLFYRVTYENGDKKVVSVDSSFIYRPNSENVIYAEFQLDKTSTSGEN
                     TIYITLDPKNKISESPAPIAELNNDYINPGGSKGYKFYI"
     gene            2792..3901
                     /locus_tag="PKV31_08110"
     CDS             2792..3901
                     /locus_tag="PKV31_08110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB92861.1"
                     /translation="MFNIVLIIGILCLIIQSVFWLACTALLNVNKRSSTEKSIVHPAV
                     SIVICARNEAENLQRNIPAFMGQKNILLEVIVVDDASTDETPQILDRMVQEFSALKII
                     RMSEKTGAGKKSALMQGILHAKYDTVLLSDADCFPASENWAEIMTAEIGSGHVIVAGY
                     SPFLEERNSLNTYARYENVMTALQYIGLGRLGLPYMGVGRNIAYQKSALAEIGYFKSH
                     SDLIAGDDDLTVQALLVKRGPKSMTFVTNEEAFVWTNAPKNRNEYFRQKLRHYSVSGH
                     YPFVIGAILSLIHISWLLFYIGLIVLLLFGSVYYFAFLLVAYVMIRLIGWQKIEKGLG
                     QRFTLKQKLVLDIIYPVLTLILTIFGLLKKNRNWT"
     gene            3892..>4226
                     /locus_tag="PKV31_08115"
     CDS             3892..>4226
                     /locus_tag="PKV31_08115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB92862.1"
                     /translation="MDIILKYFTSLTDEQLRQIEALMPLYREWNEKINLISRKDIDSL
                     YLHHVLHSLAIAKFVSFRAGARVMDLGTGGGFPSVPLAILFPETEFMAVDSIGKKVRV
                     VQDISERLGL"
BASE COUNT         1122 a          873 c         1029 g         1202 t
ORIGIN      
        1 gatgtaaaat ttataacctt tgctgccgcc gggattgata taatcattgt tcaattccgc
       61 tatcggagcc ggtgattcgg agattttgtt ttttggatca agggtgatgt agatggtgtt
      121 ttcgccggaa gtagaggtct tgtccagctg gaattcagca tatatgacgt tttcggaatt
      181 aggacggtag ataaacgatg aatctacgga tacgactttc ttatccccgt tttcataagt
      241 cacccggtaa aataaggtat cctctaccga tgtgccaagg tttttgacgg taaactgaac
      301 cgtgaaggca ttgtcagtcg atgcgatatt ctccggtatg gtcttcactt cacccgggag
      361 aattgtaaag tcaggtccgg ggaagggctg caccatcagc gccggatcgc ctacatacaa
      421 ctgttgtata ccttcctgag aattattgag gccgggcgaa ttaaaggtag cctgaaacag
      481 ttcaccaaaa gtctttccat acatgtcacc accccataag gtataaaaac ggtgggccag
      541 catactcagc gaatacagct cactgtcaga gatactgccg atgacggccc ggtagccgga
      601 gtttttgtag ctctcttcat agaggagctt ggaaggatag gacgaaccat ccctctggca
      661 attgcccgtc ttacaaccct tgataataag gataggcaga cgtgggctgt tggaatactt
      721 tgaaggcagg tctatcgggt attccagtgt ggacagggcg ccatgaccga agtaatccac
      781 gacggctaca ccctgattga ccagcttgaa aaagtcgtca gaagccttgc cgatggtctc
      841 attgctgcct ttgacaaagg tctcgacgaa gccggcaagt ttagaagact tgatcaatgc
      901 ttctacatct ttttgggcgt tagatattgc tgcctgatcc ggcttaccaa tgttgccacc
      961 attaacctga actatggtct tggtccattg cctggaagaa ttgtctcccg gtgcattgta
     1021 agcagcatca ctcgcccgga tcttattcag gtaggtaatg acttcgtctt gggttaccgc
     1081 cgggagccgg cctacagcta aattcatgtc aaccttgtgg aaggactttg acacgagaag
     1141 attgtcagat ccaaagtctc caaaggtagg cactccatag cccttgctta tcgctgttgc
     1201 caactgatta ttggaacgaa tttctttata atccctgcct ctgccaatga taaaaaggaa
     1261 gcgtgaacta cctttaaggc ctataaaatt ggcaaaattc ttgatggcca tcggattata
     1321 atccagcccg tatccaaagg aattgtaaag atcactaacg ttgaccacct caacatcgta
     1381 agcacctccg gctgcggatc ttctgtaagc ggcgtattgt tctactgcag aaggtccgtt
     1441 gttatcttcc atcagtaaat tgtgggagag tatgatatag tcggcattca ggttattaaa
     1501 atccttcatt tttacctgag tcatagatga aatagactcg gcatcgtcac cgggtctata
     1561 gatcaaccgg cttgtcactg taaatgccgg aatcctgaac cggattgtat tgccggtcac
     1621 cgagccgggt atcctccacg tccctaagga atctgtcaga taaggcgcgg tcggcacatc
     1681 accagatgca ataaaatcga gcaaacgatc ggaagccttt ccctccagga aaaaagattg
     1741 ggtcctggtg gcatcgatgt tcaggtctga cgggtattcg atagctacat accctgtttt
     1801 gataagatcc agggggtggc cgttggtgag gctgtattgg ttgtcagatg cagtaatgac
     1861 acgattggta tcgacataag taaaattgac atacatcacc cgctgtccgg tggaaaatac
     1921 actgtacatc tgctgcccct tcatagagaa tttcaggtcg tgaccaagat tgacaccgac
     1981 attgccggca ttggttatgc cgcgatagcg gaaaataggc agtctggaat ccggcaagag
     2041 gccagggcag gacaacttgt atgtttggtt gtccgcagaa gtcaccatgc cttttccggg
     2101 ataaaaaggg gtgttgacaa aggggtgctg gcctacatat tccgttttag tattcagtat
     2161 ttccctgaaa tccatttcgg atatcctgat gatgtatggt tcaggagagg gagggttgat
     2221 gaggttgtta gggaggatat tggtacggag attgtttcca tccggattga tggtgaggaa
     2281 gtaggtggcg gtatcactat acaagctggc tttgggattc acgatatcat tgacgccatt
     2341 gggaaaggaa tatttgtcca tttccgattt gttgtaataa ccgtgaaacg ttagaaagtc
     2401 actatctgac cattgtccgt tggcggatac cgtgatggga atttctaccc cgtttctgaa
     2461 caaccggagt tggctcccga ctatttcgcc gtctattcct gatgccttga gtgcattgaa
     2521 atcgatttta taaaatccat ctttgctgat cctgatctta taatattcac gacccggcac
     2581 aatccattcg ttgccgtatt ttgtaacgcc gttgtcagtc atttgagcaa atgaagcaaa
     2641 cactatgagt gaaaatagca gaatcagacc ggatctcgtc ttcatattac aaatatatat
     2701 aaattgtaat tcgtttgtac atcaaatatt atgcaaagat aactaatcaa ttgtaaattt
     2761 accatacatt tgatattatt tgctctgtag tatgttcaac atcgtgttaa tcatcggtat
     2821 cctgtgtctg attatacagt cggtattctg gttggcatgt actgccttac tgaatgtaaa
     2881 taaacgttca tctacggaaa aaagtatcgt tcatcctgcg gtttccattg tcatctgtgc
     2941 gcgtaatgaa gcggaaaatc tgcaaagaaa cataccggcc tttatggggc agaaaaatat
     3001 cctgcttgaa gtcattgtgg tggatgatgc aagtacggat gagacgccac agattttaga
     3061 ccggatggtg caggaatttt cggcattgaa aatcattcgg atgtcggaaa agacaggggc
     3121 tggaaagaaa tccgcactca tgcagggaat cctacatgcg aaatatgaca ccgttttact
     3181 ttccgatgcc gattgctttc cggcaagtga aaactgggca gagatcatga cagcagaaat
     3241 cggatcagga catgtaatag tggccggcta cagtcccttc cttgaagaaa gaaacagcct
     3301 gaatacctat gcacgctacg aaaatgtaat gactgccctg cagtacattg gcctgggcag
     3361 gttgggcttg ccttatatgg gggtaggcag gaacattgct tatcagaaaa gcgctttggc
     3421 ggaaatcggt tattttaaaa gccattctga tttgatcgcc ggggacgatg acctgactgt
     3481 tcaggctttg ctggtgaaga gaggtcctaa atccatgact tttgtaacga atgaggaagc
     3541 atttgtatgg actaacgcgc caaagaaccg gaatgaatat ttccggcaaa agctgcgcca
     3601 ttacagtgtc agtggacatt atccatttgt cattggagca atattgtctt tgatccatat
     3661 ttcatggctc cttttctata taggtttgat tgttttgttg ctttttggaa gtgtatatta
     3721 ctttgcgttt ttgttggtgg cttatgtcat gatacgtctg ataggatggc aaaaaattga
     3781 aaaagggctg ggtcagcgtt ttaccttgaa acaaaaattg gttttggata taatttatcc
     3841 ggtgcttaca ctgattctga ctatttttgg gcttcttaaa aaaaatcgca attggacata
     3901 atcctcaaat atttcacgtc actgaccgat gaacagctta ggcagataga agcattgatg
     3961 cctttgtatc gggaatggaa cgaaaagatc aaccttatat ccagaaagga tattgatagt
     4021 ctgtatctgc atcatgtgct gcatagtctg gccatagcga aatttgtttc tttcagggcc
     4081 ggagctcgcg tgatggatct tggtaccgga ggaggttttc cgtctgttcc gttagccatc
     4141 ctttttccgg aaacggaatt tatggcggtg gacagcattg gcaagaaggt acgtgtggtc
     4201 caggacatca gtgagcgcct gggtct
//
LOCUS       DAOQMC010000207         4216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10286_length_4216_cov_6.166306, whole genome shotgun sequence.
ACCESSION   DAOQMC010000207 DAOQMC010000000
VERSION     DAOQMC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10286_length_4216_cov_6.166306"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKV31_08120"
     CDS             complement(<1..476)
                     /locus_tag="PKV31_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB92863.1"
                     /translation="MKNNHYIAIMAGGVGSRFWPASREARPKQFIDMLGMGRSLLQMT
                     YDRFTPLVNPENIFCVTNQAYVNLVSQHLEELPPANIIGEPSRNNTAPSVLLTTLLIA
                     RENPDAVIGFVPSDHLIVKDQHYREICTEAFDFAQKNDAIVTLGIHPGRPDTGFGY"
     gene            complement(486..1052)
                     /locus_tag="PKV31_08125"
     CDS             complement(486..1052)
                     /locus_tag="PKV31_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB92864.1"
                     /translation="MKTPNPEGYHVVLTTHNSRTSSRMIKHGIFAGPALLLNLEQEIL
                     LTRCIGQVIIESGFRCIAYNVCRDHVHLIIVCQPEHLTGIIQKIKSVSSKNFRRNLPR
                     GQDPLDGHHPLSHSHLWSQKFYRANLDVWNLNTLTSQSGYIYKSTHLENAICYIINNR
                     KKHKLPESLELEEVISTFVVSQELAFEK"
     gene            complement(1431..2054)
                     /locus_tag="PKV31_08130"
     CDS             complement(1431..2054)
                     /locus_tag="PKV31_08130"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNB92865.1"
                     /translation="MLQTQTVEPGTLDLLKKCMELDALQPFTLVGGTALSLQYGHRMS
                     IDLDFFGNVNFLDDKLISEQFASIGNAELVNQSSVMLGFFINEIKVDIVKYKYDFIEK
                     PITEGIIRLAHPKDIGAMKLAAISGRGKRKDFYDLYYLLRDYSLEDLISFNKKKFPDS
                     NEMIIIKSLGYFDDADLDQQPILMEQVSWSTVKKTISGHLQNYIRKK"
     gene            complement(2386..3309)
                     /locus_tag="PKV31_08135"
     CDS             complement(2386..3309)
                     /locus_tag="PKV31_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNB92866.1"
                     /translation="MIKTSKIYVAGHNGMVGSAIVRKLRKVGYDNIVVRTSAQLDLRN
                     QAAVDAFFEEEKPEFVFLAAAKVGGIVANNTYRAEFLYDNLMIESNIIHAAYQHGVDK
                     LLFLGSSCIYPRIAPQPLREEYLLTGPLEPTNEPYAIAKIAGIKLCEAYRDQFGCNFI
                     SAMPTNLYGPGDNYDLEKSHVIPALLSKIHHAKVHGDATVEIWGSGNPRREFLHVDDL
                     ADACFYLMHNYEERQFVNIGCGEDISIRELAELIGKIVGFTGQFVFNTDRPDGTPRKL
                     MDVSILRKRQWSPAISLEEGLRSVYEERFLK"
     gene            3562..>4216
                     /locus_tag="PKV31_08140"
     CDS             3562..>4216
                     /locus_tag="PKV31_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB92867.1"
                     /translation="MKNFTTTLLFLLISTCIFSQTKHNISFMLDGHMRESIVVIPSHA
                     PPATGYPLVMMLHGTSGDGEKFYKISGWKELGEQENFVTVFPSSLTWCFVDDGVEKFN
                     TRWVNGNVTENPCSGAPQDYVDDVKFLKLLVSMIKDSVPINANKVFISGFSNGCAMIH
                     KMANEAGDVFAAAAGTSALLARSDSSRPVNRIPLWNVVGNQDDRFFFPPYTEVPFGRD
                     "
BASE COUNT         1194 a          853 c          845 g         1324 t
ORIGIN      
        1 atgtatccaa agccggtgtc gggacggcct gggtggatac caagggttac gatggcatca
       61 tttttctgag caaaatcaaa ggcttctgta catatttccc ggtagtgctg atccttgacg
      121 atgaggtggt cggacgggac gaatccgatg acggcatccg gattttctct ggcgatcagt
      181 agggtggtca gcaggacgct cggggctgta ttgttgcgcg acggctcgcc gatgatgttg
      241 gcgggtggca attcttccag gtgttgactg acgaggttta cataggcctg attggtgacg
      301 cagaagatat tctctggatt gaccaggggt gtaaaccggt cataggtcat ctgtaacaac
      361 gatcgtccca taccgagcat gtcaataaac tgtttgggac gtgcttctct gctggcaggc
      421 cagaacctgc tgccgacgcc gcctgccatg atggctatgt aatgattatt tttcatgctt
      481 tttatttatt tttcaaatgc cagttcctga ctcaccacaa aggtcgaaat tacttcttcc
      541 aattccaatg attcaggcaa tttatgtttt tttctgttgt tgatgatata acaaatggca
      601 ttttcgagat gtgttgactt atatatgtat ccagattgac tggtcagcgt atttaaattc
      661 cacacgtcaa gattcgcccg ataaaatttc tgcgaccaaa gatgactgtg ggagagtggg
      721 tgatgtccat ccaaggggtc ctgacccctt ggaaggttac ggcggaaatt tttactcgag
      781 acagatttta ttttctgtat aatcccggtc aaatgttccg gttggcagac aatgatcagg
      841 tggacatggt cccggcacac attataggcg atgcagcgaa aaccactttc aatgataacc
      901 tgaccaatac aacgtgtcag caaaatctcc tgttccagat tgagtaataa agccgggcct
      961 gcaaatattc catgctttat catacgggat gacgtacggc tgttgtgggt agtgaggacg
     1021 acatggtagc cttcagggtt tggggttttc ataaatgtta aaatttaggg aagttactta
     1081 taaatattca atttgtacaa atttagaaaa ttttgacaaa tactatcctt tattttttca
     1141 gaatagttta aaacaaatta ctcttttctg aattttaatg ccggttcgac aatggatcaa
     1201 ttaaaaagct taacgctcga tatttaaggc tctactttat aactcttcgt tatttttggg
     1261 caattcatcg tctcattcgt taaatttaac aagcctgtca gagggcttac atccgacgga
     1321 aatttgtttt ttatttgtga aatatatcat acaaaaaatt ccaggttgca tcatctgaaa
     1381 tcagattttc acctatttgt tgggcatgta gaaattctga tggttgagaa tcattttttg
     1441 cggatataat tttgaagatg gcctgaaatc gtctttttaa cagttgacca tgatacttgt
     1501 tccattaaaa taggctgttg gtcaaggtca gcatcgtcaa agtatccaag actttttatt
     1561 attatcattt cattactatc agggaatttt ttcttattaa aactaatcaa atcttccagg
     1621 gaataatccc tcaaaagata gtaaaggtca taaaagtctt ttcttttgcc tctacctgaa
     1681 attgctgcta atttcattgc tccaatatct tttggatgag caaggcggat aattccttct
     1741 gttataggtt tttcgatgaa gtcatattta tatttcacta tatcgacttt aatttcatta
     1801 atgaaaaaac ccaacataac actagattga tttacaagtt cggcgtttcc tatagaagca
     1861 aattgttctg aaataagttt atcatcgaga aaattaacat tgccaaaaaa atcaaggtca
     1921 attgacattc tatgaccata ttgtaaggaa agtgctgtac cgcctacaag ggtgaatggt
     1981 tgaagggcat ccaactccat acattttttc aataaatcca gcgtccctgg ttcaactgtc
     2041 tgtgtttgta acatctgaaa tcagattttt gtaatccgga aagtatcacc aaaaatgata
     2101 aagtcttcgg gctgagatat cttattcctc tgcttatttc aatgatatta tcaacgttat
     2161 aaacctgttt tataatttgc caatcgttcc aatttccata ctccaatact ctttcgatta
     2221 taaaagcagc attttggtcc cactgaagat cttctttttt cgtatcccag aagatttgac
     2281 tggaaaggtc aattatattg taatttgtag tcattcttga gatttggtaa gatatacttc
     2341 aaagataaca aaaattatac ttaaaaaatt acctgcaatt agtcgctatt ttagaaacct
     2401 ctcctcatac acacttctca atccttcttc cagactgatc gccggactcc actgcctttt
     2461 tctcagaata cttacatcca tcagctttct gggagtgccg tcagggcggt cggtattgaa
     2521 gacaaactga cctgtaaatc ctactatctt accgatgagt tcggcgagct ctctgatgga
     2581 gatatcttca ccgcaaccga tattgacaaa ttgtctttct tcataattgt gcatcagata
     2641 aaaacaggca tctgccaggt catccacatg gagaaattcc cggcgcggat tgccgcttcc
     2701 ccatatctcc accgtcgcgt caccatgcac cttagcatga tgtattttgc tgagcagggc
     2761 agggatgacg tgtgactttt caaggtcata attgtcgccg gggccgtaaa gattggtcgg
     2821 catggctgaa ataaaattgc atccgaactg atcacgatag gcctcacaca gcttaattcc
     2881 ggcaatcttg gcaatggcat acggctcatt ggtaggttcc agcggaccgg tcaacagata
     2941 ctcttcccga agcggttgag gcgcaatcct gggatagata cacgaactcc ccagaaacaa
     3001 caacttatcc acaccgtgtt gataagcggc gtggatgatg ttggattcta tcatcaggtt
     3061 gtcgtataaa aactcagcac gataggtatt gttggcaacg atgccaccca cttttgccgc
     3121 tgcgagaaaa acaaattccg gcttctcttc ttcaaaaaat gcatcgacag ctgcctgatt
     3181 ccgaagatcc agttgagcac ttgtcctgac gacgatgttg tcataaccaa cctttctgag
     3241 tttgcgcaca atagcagaac cgaccatccc attgtgtccc gcgacatata tttttgaggt
     3301 tttaatcatg aaatattttt tgtttaaatt gactttcaaa ataaatttga aatatatgaa
     3361 aatttgaaat attcaatcca gggatatcta gtaccaagtg gattattgaa taaatattaa
     3421 tttcctgcta aaatataatc tatttcctgt agaaaaaaag ttaaaatgaa gtatcagtaa
     3481 tataattgtt cttaattaaa aaactattta attgatatat tttcaagtat tattgttaat
     3541 tttgaacaaa aatcattcgc catgaaaaac tttactacaa cactcctctt ccttctgatt
     3601 tcaacctgta ttttttcaca aacgaagcac aacatcagtt ttatgctgga tggacatatg
     3661 cgagaaagta tcgtcgtcat tccgagccat gcgcctcctg ctaccggtta tcccctggtc
     3721 atgatgctcc atggtaccag tggtgacgga gaaaaatttt acaagatctc cggttggaaa
     3781 gaactgggag aacaggaaaa ttttgtcact gtatttccat cttccctcac ctggtgcttt
     3841 gttgatgatg gggtagaaaa atttaacacc agatgggtca atggtaatgt taccgaaaat
     3901 ccttgttccg gagcaccaca ggattatgtg gatgatgtca aatttttgaa attattggta
     3961 tctatgatta aagactcagt gcctatcaat gccaataagg tatttatcag cggtttttcc
     4021 aacggttgtg ccatgatcca caaaatggcc aacgaggccg gagatgtctt tgcggctgcc
     4081 gccgggacca gcgccctatt ggcccgatca gattcttcgc ggcctgtcaa tcggattccc
     4141 ctttggaacg ttgtcggaaa tcaggatgac agatttttct ttcctcctta tacggaagtg
     4201 ccttttggaa gagatt
//
LOCUS       DAOQMC010000208         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10559_length_4152_cov_3.933610, whole genome shotgun sequence.
ACCESSION   DAOQMC010000208 DAOQMC010000000
VERSION     DAOQMC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10559_length_4152_cov_3.933610"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKV31_08145"
     CDS             <1..843
                     /locus_tag="PKV31_08145"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HNB92868.1"
                     /translation="DIDFIIFATLSPDYFFPGCGVLLQRELGITDTECGALDVRNQCS
                     GFIYALSVAHQFASSGMYRNILVVGAEVHSMGLDYTTRGRNVTVIFGDGAGAAIVQPT
                     AESGEGILATCMHSNGTHAEELAFINPGCHGGRYGGIENYGYLDEKEYGGTFLTQKMM
                     DDALIYPNMNGQLVFKNAVQKMPEAIMEVLNKAGYKPSDLKMLIPHQANLRIAAFVQH
                     TLRLNDDQVYNNIQKYGNTTAASIPIALTEAYQQGLFSSGDLVCLTAFGSGFTWGAVL
                     MRWA"
     gene            1038..1238
                     /locus_tag="PKV31_08150"
     CDS             1038..1238
                     /locus_tag="PKV31_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92869.1"
                     /translation="MKKILTYIVLFVSIAAFSASVSSCSRKSGCPAYDEAVSKPKKNG
                     NFNKGKSQLFDKKMRKRMAQRG"
     gene            1336..>4152
                     /locus_tag="PKV31_08155"
     CDS             1336..>4152
                     /locus_tag="PKV31_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HNB92870.1"
                     /translation="MESGKQYNLLIQKLDQFIRKFYLNRIIRGSLYLLGFVTVYFMAI
                     ALLENFFYFGTAGRKFLFYSFLLASLFTLIRWIIVPAMKFFRLGSTISNEQAAAIIGD
                     HFGNVKDKLLNVLQLNEQARNNPDSSLLIAGIEQKTKELQPIPFKNAIDLSSNKKYLK
                     YSVPPVLALLLMLFTAPSLIKKPVNRLINNDKEFEREAPFTFQILNKNLKVAEFDNYT
                     LEVKLEGNSIPDQIDVVYHDNPYRLVKDSRGIFTYTFANVQDAFDFRLKAAGFNSKVY
                     RLDVMKKAGMTDLNLKLVYPGYIGRPEEYVKGSGDIIVPFGTKVYWDIRTRLADRVEL
                     EFEGNHAVSAQRAGDNRFTFLKTAIQDQNYKIFTGNQALPVSDSMTYSIHVVMDENPV
                     ISLDQYVDSANQKLVYLSGNVSDDYGFTKLQMVQQIKSEDGKVRTRTLPLQKPATKQG
                     FYSYLFDIESLKLKPGDELTYFFEVFDNDGAKGPKSARTSVMNFKKPSVKEMEKNIDK
                     STREIQSDLEKMVKESKKVNDAINKLREKLLQQKSLNWQDKEQLNKLLEKQKELQDQL
                     KETQKKLEEKRKAEEEIKQPDPEMKEKQEKLDEMIKEMQNPEEQKLMDKIRELMDKLD
                     KKESLKQLEQMSQQNEQQHKSLDRLMELMKQLELEAKVQDQIDKLNELAEKEEKLAEQ
                     TENNQKDQEQLKKEQDQINKDFQEVKKKMDEIQKENKELKAPKKLNDQKEEQQSTEQD
                     LQDAKEQLEKKENKSASKSQKKAGQKMKQMAQNMQNDMAASEKEQIELDMKAVRQLLE
                     NIVGLSFNQEDLIKQYNATMINTPRYIGLNREQKKIKSDFKMVDDSLQELSKRVMQIQ
                     SFIIKKVAEVNDNLDKTMDNLEDRRKPEAAVNQQQSMTSLNDLALMLSEALQQMQMQA
                     QSMPGNASCSKPGGSNPQDGKTGKP"
BASE COUNT         1344 a          799 c          984 g         1025 t
ORIGIN      
        1 gacatcgact tcatcatctt cgctacgctg agcccggatt acttctttcc cggatgtggg
       61 gtgttgttac agcgggagtt gggcatcact gacactgaat gtggcgcctt ggatgtcaga
      121 aaccaatgtt ccggctttat atatgcgcta agtgtggcgc atcagttcgc ttcttcaggt
      181 atgtacagga atattcttgt ggtgggcgcg gaagtgcatt ccatgggtct cgattatacg
      241 acaagaggca ggaatgtgac cgttatcttc ggtgatggtg ccggagcggc catcgtacaa
      301 ccaacagcag aatcaggaga gggtatctta gctacttgta tgcacagtaa tgggacgcac
      361 gcggaagagc tggccttcat caatccgggt tgccacggcg gccgatatgg cggtattgaa
      421 aattacggtt atctcgatga gaaggaatac ggaggtacct ttctgacaca gaaaatgatg
      481 gacgatgcat tgatatatcc taatatgaat ggacaattgg tgtttaagaa tgctgtccag
      541 aagatgccgg aggccatcat ggaagtattg aataaggcag gatacaaacc atcagacctg
      601 aagatgctga ttccacatca ggccaatctg cgtattgcgg cctttgttca gcacaccctt
      661 agactcaatg acgaccaggt gtacaacaac attcagaaat acggcaacac tacagccgct
      721 tccatcccta ttgcactgac agaggcctat cagcagggct tgttctcttc cggagacctc
      781 gtatgtctta ctgcctttgg tagtggcttt acctggggtg cagtgttgat gcgctgggca
      841 taaatccaat tagatctata ttagaattac ggaatagcat ttgcatagag attaccaaat
      901 aattaatcat tgaaccttct tgaagataaa gtgttaatta tgccgttaat catgttgatt
      961 acgaactatt tagattttaa cagcgtttta tagggagaaa ttcaatttaa catgtagtcg
     1021 ctgacaagca tttgcctatg aaaaaaatac tgacgtacat tgtccttttt gtttccattg
     1081 ctgctttttc tgcatcagtg agctcctgct caaggaaatc gggatgccct gcttatgatg
     1141 aagctgtttc gaagccgaag aagaacggaa attttaataa aggtaagtct cagctttttg
     1201 acaagaaaat gcgtaaaaga atggcgcaga gaggctgatt cgggacttaa tttaattttt
     1261 atcacatcat tgatctttat aatctgaaac gaaactgtcg gattttcggt taaaataatt
     1321 tggagttgat tatttatgga gtcaggaaag caatataatc tattgataca aaaactggat
     1381 caatttatcc gcaagtttta tctgaacagg attattcgtg gttcgcttta tttgctgggc
     1441 tttgttaccg tatatttcat ggcaatagcc ttgctggaga attttttcta tttcgggact
     1501 gcgggccgca agttcctttt ttactcattc ttacttgcct ccctgtttac tcttatacgg
     1561 tggatcattg ttccggctat gaagtttttc cggttaggct ccaccatttc caatgaacag
     1621 gcggctgcca tcataggaga tcattttggc aatgtcaagg acaaattact caatgtattg
     1681 caactcaatg agcaagcacg taacaacccc gattcctccc ttttgatcgc aggcatagag
     1741 caaaaaacaa aagagttgca gccaattccc tttaaaaatg ccattgacct gagctccaat
     1801 aaaaaatatc tcaagtatag tgtgccgccg gtattggcgc tgcttcttat gctttttacc
     1861 gccccctccc tgattaagaa gcctgtcaat cgattgatca acaacgataa ggagtttgaa
     1921 cgggaggcac catttacctt ccagatactg aacaaaaacc tgaaggtcgc cgagtttgat
     1981 aactatacac tggaagtcaa attggagggg aattcgattc cggatcagat tgatgtggtc
     2041 tatcatgata atccatacag gttggtcaag gatagtcggg gcattttcac ctacaccttt
     2101 gctaatgtcc aggatgcttt tgatttcaga ctgaaggctg ccggatttaa tagcaaggtg
     2161 tatcgattgg atgtaatgaa aaaagcggga atgacagacc tcaacctgaa actcgtctat
     2221 ccgggctata ttggaagacc tgaggaatac gtcaaaggct ccggagacat catcgtccca
     2281 ttcggcacaa aagtatactg ggatatcagg accagacttg ccgatcgggt tgagttggaa
     2341 tttgaaggca atcatgccgt gagcgcacaa cgggcgggcg acaatagatt tacatttttg
     2401 aagacagcca tacaggatca aaattataag atcttcacag gcaatcaggc actcccggta
     2461 agtgacagca tgacatattc gatccatgtc gtgatggatg aaaaccctgt aatcagtctt
     2521 gatcaatatg tggattcagc caaccagaaa ctggtctatc tcagtggcaa tgtctctgat
     2581 gattatggct ttacaaagct gcaaatggtg caacagatca aatccgaaga tggaaaagta
     2641 agaaccagga cactgccact acagaaaccc gcaactaaac aaggattcta tagctatctt
     2701 tttgatatag aatcgttaaa gttgaaaccg ggtgatgaat taacttattt tttcgaggta
     2761 ttcgacaatg atggtgccaa agggcccaaa tctgccagga cttcagtgat gaactttaag
     2821 aagccttctg ttaaggagat ggagaaaaat atcgataagt ccaccaggga aatccaaagt
     2881 gatctggaaa aaatggtgaa ggaatcgaaa aaagtcaatg atgcaatcaa taagctcagg
     2941 gaaaaattac tgcagcaaaa gtcattgaat tggcaggata aagaacagtt gaataagttg
     3001 ctggagaaac aaaaggagct tcaagaccaa ctaaaggaga cccagaaaaa actggaggag
     3061 aaacgcaagg cagaagaaga gataaagcag cctgaccctg agatgaagga aaaacaggag
     3121 aaacttgatg aaatgatcaa ggaaatgcag aacccggaag aacaaaagtt gatggataag
     3181 atccgggagt tgatggataa actggataaa aaggagtccc tcaaacaact ggagcaaatg
     3241 tctcaacaaa atgaacagca gcataagagt ctggatcggc ttatggaatt gatgaagcag
     3301 cttgaactgg aagctaaggt ccaggatcag atagataaac tgaatgaact ggccgaaaag
     3361 gaagaaaagc ttgctgagca gactgagaat aatcaaaagg atcaagagca acttaagaag
     3421 gaacaagacc agatcaataa ggactttcaa gaggtgaaga agaaaatgga tgagatacag
     3481 aaagaaaaca aggaattgaa ggcgcctaag aagttgaatg atcaaaaaga agagcagcaa
     3541 agtacggagc aggaccttca ggatgccaag gaacaattgg agaaaaaaga aaataaatcc
     3601 gcttctaaga gccaaaagaa agccggccag aagatgaagc agatggctca gaatatgcag
     3661 aatgatatgg cggcatctga aaaggaacag atagagctgg atatgaaagc cgtccgtcag
     3721 ttgctggaaa atatagttgg tctgtcattt aatcaggaag accttatcaa acagtataat
     3781 gcaaccatga tcaacacacc gagatacatc ggtctgaaca gggaacagaa aaaaatcaag
     3841 tctgacttca agatggtgga tgacagtctg caggaactta gtaagcgggt tatgcagata
     3901 cagtcgttta tcatcaagaa agtcgctgaa gtcaatgaca acctggacaa gactatggat
     3961 aatctcgaag acagacgtaa gccggaggct gctgtcaatc aacagcaatc aatgacaagc
     4021 ctcaatgatc ttgcacttat gctaagtgag gcattgcagc aaatgcagat gcaggcacaa
     4081 tctatgccgg gtaatgccag ttgtagcaaa cccggaggaa gtaatcccca ggatgggaag
     4141 accggcaaac cc
//
LOCUS       DAOQMC010000209         4138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10622_length_4138_cov_4.415871, whole genome shotgun sequence.
ACCESSION   DAOQMC010000209 DAOQMC010000000
VERSION     DAOQMC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10622_length_4138_cov_4.415871"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PKV31_08160"
     CDS             complement(<1..921)
                     /locus_tag="PKV31_08160"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HNB92871.1"
                     /translation="MKHPISILLLIFFIHDAFSQQIASPTPPMGFMTWNYFKDNLTEN
                     DVRTIADALVSTGLRDLGFNHIFIDDGWQGGRDNRNNLIPDPVKFPSGMKTLSDYVHS
                     QGLKLGIYSDGAPLTCAGYTGSLHFEDQDARTFAQWGIDYLKYDYCNAPSDRETAIAR
                     YAKMAKALKSCGREIILGICEWGDREPWLWAGKAGGQLWRTTADVRDKWRSKTKPETP
                     ADLHHLGAGIFDIMNVNASLNKFAGPGGWNDADMLVVGLNGKEGPSGDLGGTGCSDIE
                     YQSQMSLWCLMAAPLMISCDIRNMNTTTKMI"
     gene            complement(1076..2713)
                     /gene="groL"
                     /locus_tag="PKV31_08165"
     CDS             complement(1076..2713)
                     /gene="groL"
                     /locus_tag="PKV31_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNB92872.1"
                     /translation="MMAKEISFDSAARNELKAGVDALANAVKVTLGPKGRNVVIQKSF
                     GAPTVTKDGVTVAKEIELENPVQNMGAQMVKEVASKTADAAGDGTTTATVLAQAMVTA
                     GMKNVTAGANPMDLKRGIDKAVKEVISDLHKQSEVIGDDFNKIKQVGSISANNDEQIG
                     TLIADAMKKVSKDGVITVEEAKGTDTYVDVVEGMQFDRGYLSAYFINNPDKMLAEYEH
                     PYILIHDKKISNMQDIVPVLEKTMQSGRPLLIIAEDIDSQALGILVVNRLRANLKIVA
                     VKAPGFGDRRKAMLEDIAILTGGTVISEEQGYKLENVELEHLGTAEKITVDKDNTTII
                     GGAGTQEEIQVRINSIKQQIEITTSDYDREKLQERLAKLSGGVAVLYVGAPTEVEMKE
                     KKDRVDDALHATRAAVEEGIVAGGGVALVRALDAIKDLKGDHEDETIGIQIVRKALES
                     PLRTIVENAGMEGSVVLQKVIEGSGSFGYNARSDKYEDLKLAGVIDPTKVTRIAIENA
                     ASIAGMILTTECVINDKPKEDAGHDHPAGMGGMGGMM"
     gene            complement(2764..3033)
                     /locus_tag="PKV31_08170"
     CDS             complement(2764..3033)
                     /locus_tag="PKV31_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNB92873.1"
                     /translation="MDMKPINDRVVVKPAPAEEKTKGGIIIPDTAKEKPQRGEVIAVG
                     PGKDGNLMTVQVGDIVLYGKYAGQELNYQGEEFLIMREDDILVIL"
     gene            3345..>4138
                     /locus_tag="PKV31_08175"
     CDS             3345..>4138
                     /locus_tag="PKV31_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB92874.1"
                     /translation="MRLNIILSGILLVLFGACNSSVKIRTGEAAFDVGQYSVAVEMLK
                     EEIEKTENKALKAEKAFLMGKAYEMMNDPDNALVWFRKGIEGSDSPTRMVYLAYALKN
                     TGRYAEAMQVFNELSKQLNDPNRFRSEIVSLNNAFQWQKDAVTDPYKLTLCPFNSADA
                     DYSPVISKEGKIIFTSDRKQDDAEAPYKWTGRGFSNIFVADSGGSEALPLSGLVNTTD
                     NEGLLVYTQAGDEMAFCRCFDRVGGNSFCKILISRYVDGSWTDPQV"
BASE COUNT         1027 a          928 c          904 g         1279 t
ORIGIN      
        1 aatcattttg gttgtggtgt tcatattgcg gatatcacag gatatcatga gcggagcagc
       61 catcaggcac caaaggctca tctggctttg atattcgatg tcagaacaac ccgtgcctcc
      121 aagatctccg gaggggcctt ctttcccatt aagacctaca acgagcatat cagcgtcatt
      181 ccagccaccc gggccggcaa atttgttaag gcttgcgttg acattcatga tgtcgaatat
      241 tcctgcgccg agatggtgca gatcggccgg agtctccggt ttggttttac ttcgccattt
      301 atctctgaca tcggctgtgg tgcgccataa ttgcccacct gcttttcccg cccaaagcca
      361 aggttcacgg tcaccccatt cgcagatgcc gaggatgatt tcacgcccgc atgatttaag
      421 tgctttggcc atttttgcat atcttgctat ggctgtttcc cgatcggaag gcgcattgca
      481 ataatcatat ttgaggtaat caatgcccca ttgggcaaag gtgcgtgcat cctgatcctc
      541 gaaatgaagg ctaccggtat aaccggcaca ggtcagtggt gcaccatcgg aataaatacc
      601 cagcttcagt ccctggctgt gcacatagtc gctgagggtt ttcatgccgg agggaaactt
      661 gaccggatcg gggatgagat tgtttcggtt gtccctacct ccctgccatc cgtcatcaat
      721 gaagatatga ttaaatccaa gatccctcag cccggttgac actaaggcat ctgctatggt
      781 acgaacatcg ttttcagtga ggttgtcctt gaaataattc caggtcatga agcccatggg
      841 aggggtgggg gaggctatct gctggctaaa tgcgtcgtgg atgaaaaaga ttagtaaaag
      901 gatcgaaatc ggatgcttca tttgtgagaa tgagattgat acaatgcgtc aagatacgca
      961 aaataacaat tatcgcgatg atcagcccat ctctattttt cgaattgcct tatggttttt
     1021 acaaaaaaag gccggcaagt gccggccttt ttttgatatt tcaaggggaa aatgattaca
     1081 tcattccgcc cattccgccc attccggccg gatggtcatg ccctgcatct tcttttggct
     1141 tgtcgttgat aacacactcc gtagtgagga tcattccggc tatagaagcg gcattttcaa
     1201 ttgctatcct tgttactttt gtaggatcta tgacacctgc aagcttaagg tcttcatatt
     1261 tgtccgaacg cgcattatat ccgaagcttc ctgagccctc aataaccttt tgcagcacta
     1321 cagaaccttc catgcctgcg ttctcaacaa tggtacgaag aggagactcc agtgcttttc
     1381 taactatctg gataccgatg gtttcatctt catgatcacc tttcagatct ttgattgcat
     1441 ccagtgctct gactaacgct acgccaccac ctgcaacgat accttcttca acggcagctc
     1501 ttgtagcatg taatgcatcg tcaacgcggt cttttttctc cttcatttcg acttctgttg
     1561 gagcaccgac ataaagaacg gcgacaccac cggacaattt agccagacgc tcctgaagct
     1621 tttcccggtc gtagtcggaa gtggtgattt cgatttgttg cttgatggag ttgatacgaa
     1681 cctggatttc ctcctgagtt cctgcgccac caataatggt cgtgttgtcc ttatctactg
     1741 taattttttc agcagtacca agatgctcta gttcaacatt ttccagtttg tagccctgct
     1801 cttcagagat tacagttccg cctgtcaaaa tggcaatatc ctcaagcatg gcttttcttc
     1861 tgtcaccgaa gccgggagct ttgacagcaa cgattttaag gttggcacgc aatctgttga
     1921 caaccaaaat gcccaaagcc tgactgtcta tgtcttctgc aataatcaac aatggccttc
     1981 ctgattgcat ggtcttttca aggacaggga cgatgtcctg catgttggag attttcttgt
     2041 cgtggatcag gatatatgga tgttcatatt ctgccagcat tttatccgga ttgttgatga
     2101 aatatgcgga taagtatcca cggtcaaatt gcataccttc caccacatca acataagtgt
     2161 ctgttccctt agcttcctca acagttatta ctccgtcttt agaaactttc ttcatagcat
     2221 ccgcgataag ggttccaatc tgctcatcgt tgtttgcaga gatcgaacca acctgcttga
     2281 tcttgttaaa atcatcgccg atgacttctg attgcttatg caggtcactg ataacttcct
     2341 taacagcttt gtcaatacct cttttcaggt ccattggatt ggcacctgca gtaacgtttt
     2401 tcattcccgc cgttaccatt gcttgagcaa gtactgttgc agtggttgtt ccatcgccgg
     2461 ctgcatcagc agttttggaa gcaacttcct ttaccatttg agcacccata ttctgaaccg
     2521 gattttccaa ttcaatttcc tttgccacgg taacaccgtc cttggtaaca gtcggtgctc
     2581 cgaagctttt ttgaataact acattacgac ctttaggtcc aagagttact tttactgcat
     2641 tggccaatgc atccacaccg gctttcaatt cattgcgcgc tgcgctatcg aagctaattt
     2701 ctttagccat aatactttag atttttaatt ttaacaaatg agtaaataga atgtattagc
     2761 gagttacaga atgacgagaa tgtcgtcttc acgcatgatc agaaattctt ccccctggta
     2821 gtttaattcc tgaccggcat attttccata aagaactatg tccccgacct gtacggtcat
     2881 aagattgcca tccttgccgg gacctaccgc gatcacttca cctctttggg gcttctcttt
     2941 tgccgtatcg gggataatga taccgccttt ggttttttct tcagcaggag caggcttcac
     3001 tactactctg tcgttaattg gtttcatgtc tatgttctat ttaattttta caaaaatatt
     3061 tttcgcttac ccttaagcat aaaccatgcc aatggaaggt gatgtgacaa tttttcgttt
     3121 tctttgccat tgtgtcagtt gctgtgtgtt ttaattggca tgtatcgtta ttattgttgc
     3181 atatattaat taacggttat aatgttggtt cttacaggaa ttttcatatc ttagtaattt
     3241 gatgtattgc cagaactttg aaatttgttt aagcgtcttg agtttctata atagtcagta
     3301 gcattgggaa tggcatatgt ttttaaatcc gaatactttt ttagatgcgt ctaaatatta
     3361 ttttatctgg tattctgtta gtcctctttg gggcatgcaa tagttcggtt aagattcgaa
     3421 ccggcgaagc agcttttgat gttggtcagt attccgttgc agtggaaatg ctgaaggaag
     3481 aaattgagaa aacggagaac aaggctttga aggccgaaaa ggctttcctg atgggcaagg
     3541 cttatgagat gatgaatgac cctgacaatg cccttgtctg gttcagaaag ggcatcgaag
     3601 ggagcgactc tccgacccgc atggtgtacc ttgcctatgc tttgaagaat acaggtcgat
     3661 atgcagaagc aatgcaggtt ttcaatgaac tcagcaagca attgaatgat ccgaatcggt
     3721 tcaggtcaga aatcgtttcc ttgaataatg cttttcaatg gcaaaaagat gctgttacag
     3781 atccctataa actgacttta tgtcccttca atagtgctga tgcagattat tcaccagtca
     3841 ttagcaagga gggcaaaata atatttactt cagatcggaa acaggatgat gcggaagctc
     3901 cttacaaatg gaccggaaga gggtttagca atatttttgt tgcagattcc ggtggtagcg
     3961 aggcattgcc attatccggg ttggttaata cgacagataa tgaaggattg cttgtatata
     4021 cccaggcagg agatgagatg gcattttgcc gctgctttga cagagttggc ggaaattcct
     4081 tttgcaaaat cctgatatca aggtatgttg acggaagctg gacagatcct caggtatt
//
LOCUS       DAOQMC010000210         4126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10687_length_4126_cov_4.849914, whole genome shotgun sequence.
ACCESSION   DAOQMC010000210 DAOQMC010000000
VERSION     DAOQMC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10687_length_4126_cov_4.849914"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(782..1636)
                     /locus_tag="PKV31_08180"
     CDS             complement(782..1636)
                     /locus_tag="PKV31_08180"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNB92875.1"
                     /translation="MKNNIFRLCILTGMALGLWSCQETEIVVPRVKEIVSDRVVLIED
                     FTGVQCTNCPNASREISSLAHKYNGNVVAIAYHTDFLGLPITKDGYKSKYNFTTPDGN
                     LLEEEMGGYQGKPAVTLNRKLYDAQQEYLLTSSSILGNIESELRSIPRVVIDIENQYD
                     AETRKLRTKVDIKPQAVASGDFRLHVCITEDKIIDSQIDNIVYVKDYEHNHVFRTMLS
                     TLAGDILGTDLVPGSNLSRTYEYTLPPEAGWWVASHCNVVAFVTDLSQKAFNVGAVLQ
                     AAEKHVIE"
     gene            complement(1647..3296)
                     /locus_tag="PKV31_08185"
     CDS             complement(1647..3296)
                     /locus_tag="PKV31_08185"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNB92876.1"
                     /translation="MQKLALTFFGFFLVLWQGYGQGITGGKVSGGLQTNLNFFMKDAS
                     INAANTPQYETQLYSADSWLNVNYNTKGFEAGIRLDVYNHSYLFNPNGGSYTGIGIGN
                     WYVAKQIDKLNIAGGYLYDQIGSGIIYRAYEERPLAIDNALFGVRASYDLTPNWRVKG
                     FTGQQKFQFDRYGAIIKGASIDGFITGSDSAAKHTLTLSPGAGVVARTFDNPTIEGIN
                     NVLKYYEPIDSIATYKSNTYAMTVYNTLSYGPFTLYTEAAYKTPEVMYSPTEFRHIGL
                     DSSAGKFIKAAGTVFYGSLSYADHGLGINIEGKRTKNFSFRTDPNLLLNRGLINYIPA
                     MSRVNTYRLTSRYNPAIQFMGEYAFQVDVQYRFNDHWNALINYSHINDLNDVKLYREI
                     YGEVTFKPNKKNQFILGLQRQEYNQYIYEGKVSVPNVETFTPYIEYLHKFNRKTAIRV
                     EGSMMATRQDYGSWAFGLVELTIAPHWSFVIADMYNYDYNPEKTKKANNYPSIIAFYT
                     RGSNRFSLGYIKQVEGIVCTGGICRYEPAFSGVKATVSTTF"
     gene            3595..4071
                     /locus_tag="PKV31_08190"
     CDS             3595..4071
                     /locus_tag="PKV31_08190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB92877.1"
                     /translation="MMLFVIGVSTQAFGQTDLPSTRVKTLDGKSVTLQEVIKPGVITV
                     ISFWATWCAPCKKELDAINEIYEDWQSEYGVELIAISTDDARTAPKIKPTITQKGWKY
                     TFLNDQNQELQKALNFQAVPQTFLIGKDGKIAWTHAGYVPGDENELENHIKELAGK"
BASE COUNT         1129 a          901 c          891 g         1205 t
ORIGIN      
        1 ctcaaatgct tcgtctaaaa atattacttc aaaaagtttg cccattcgtt aaattgccaa
       61 caaagcaaag acaataaaag tttcgttata atgaaacatt gggtcgattt attttgtcat
      121 gattggtcgg caaatatggc atagaacggt ttcgggcttt gagcaattgt tcggtggatt
      181 gttggttggt aataatattt tcttttaatt gttgggtaaa ggcaatatcc tttttttcaa
      241 ctaccccaac ggggtatcat gtttatagaa acatgattat aatgaatgtg cgaccctgta
      301 ggggtcgaat aaaattgttt tacaattttt ctataaacat taaatccctt caggattatg
      361 cgtattccgt ttccgtcaca tcaaatgcct gatgtagcaa ggcttttaac aattgttcgc
      421 tggattgttg gttggtaata atattttctt ttaattgtta tgtaaggaca atatcctttt
      481 cttcaactac cccaacgggg tatcatgttt atagaaacat gattataatg aacgtgcgac
      541 cccaacaggg gtgtgatgca ataggacaaa tgcaatttct atatcattca atggggcatc
      601 aatggaaagt tggatttcat ccctcatcag tctgtacagt atggacaatg caaatgaagc
      661 cggattgaat acccaccccc ttggcaggtg tcaaccccat aaccaaattc aaactttctt
      721 tatttgaaaa aaatatttag ctcatcctgc ggacgacata tcagtctcga ccgctaatgc
      781 gctattctat tacatgcttt tcagctgcct gcagtactgc ccctacattg aaagccttct
      841 gtgacaagtc agtaacgaat gccacaacat tgcaatggga cgctacccac caacccgctt
      901 ccggcggaag ggtgtactca tatgttctgc tgagattact gccaggcacc agatctgttc
      961 cgaggatatc acccgcaagt gtgcttaaca tcgtcctgaa gacatggttg tgctcatagt
     1021 ccttgacata tactatattg tcgatctgag aatctatgat cttatcttcc gtgatgcata
     1081 catgaagccg gaagtcgccg ctcgccacag cctgcggttt gatatccacc tttgtgcgca
     1141 acttccgcgt ctctgcatcg tattggtttt caatatcaat gactaccctg ggtatacttc
     1201 tcaattctga ttcgatattg cccaaaatag aggatgatgt gagcagatac tcttgctgag
     1261 catcatacaa tttcctgtta agagtgactg ccggcttgcc ctgatagcca cccatctctt
     1321 cttccagcaa gttgccgtcc ggtgtagtaa agttgtattt actcttatat ccgtccttgg
     1381 tgattggtag cccgaggaag tcggtatgat aagcgattgc caccacatta ccattgtatt
     1441 tatgtgcaag actggagatc tcccggctgg cattcggaca attggtgcat tgtactccgg
     1501 taaaatcctc gatcaatacc actctgtcgg atacaatctc tttgacacga ggcacgacga
     1561 tttcagtttc ctggcagctc caaagtccga gtgccatacc tgtcagaata catagtctaa
     1621 aaatattatt cttcattttg aaaaaattaa aaggtggtcg aaacagttgc cttcacgccg
     1681 ctgaatgccg gctcataccg acatatacct cccgtacata caatgccctc cacctgcttg
     1741 atatagccca atgaaaagcg gttgctgccg cgtgtataaa aggcgataat ggacgggtaa
     1801 ttattggctt tttttgtttt ctcggggttg taatcataat tgtacatatc agcaatgaca
     1861 aaactccagt gtggcgctat ggtcagctct accaggccaa atgcccagct tccatagtcc
     1921 tgcctggtag ccatcatcga gccctctacg cgtatggctg tcttgcgatt aaatttgtgt
     1981 agatactcta tataaggggt gaaggtctct acattgggca cgctcacctt gccttcatat
     2041 atatactgat tgtactcctg acgctggaga cccaggataa actggttctt cttgttgggc
     2101 ttgaaggtca cttctccgta tatctcccga tatagcttga catcattgag atcgttgata
     2161 tgagaataat tgatcagggc attccaatgg tcattgaatc tatactgcac atcgacctgg
     2221 aaggcgtatt ctcccataaa ctggatggca gggttgtatc tggatgtcag ccgataggta
     2281 tttactctgg acatcgccgg aatatagttg atcaggccgc gattgagcag caggttagga
     2341 tcggttcgga atgaaaaatt cttagtacgc ttgccttcaa tattgatgcc cagaccatgg
     2401 tcggcatagc tcaaactgcc atagaataca gttccggccg ccttgatgaa tttcccggct
     2461 gaagaatcca ggccgatatg tcgaaattca gttggagaat acatgacttc cggtgttttg
     2521 tacgctgcct ccgtataaag cgtaaaggga ccataactca gcgtattgta tactgtcatc
     2581 gcataggtat tggacttata ggtagcaata ctgtcgatgg gttcgtaata tttaagtaca
     2641 ttgttgatgc cttcgatggt cgggttgtcg aatgtcctgg ctacaacacc ggcacccggg
     2701 gacagagtaa gcgtatgctt ggctgcgctg tcgctgcccg tgataaaccc atcgatgctt
     2761 gcgcccttga tgatggcccc ataacgatca aactggaatt tctgctgtcc tgtaaaaccc
     2821 ttgacccgcc agttgggtgt cagatcatac gatgcacgta caccaaacag ggcattgtcg
     2881 atggcaaggg gacgctcttc ataggcccgg tagatgatgc ctgatcctat ctggtcatac
     2941 aggtatccgc cggcaatatt cagcttgtct atctgcttgg ccacatacca gttgccgata
     3001 ccgatgccgg tataagagcc gccattggga ttgaacaagt aagaatggtt atatacatcc
     3061 agtcgaatcc ctgcctcaaa cccctttgtg ttataattga cattgagcca gctgtccgca
     3121 ctgtaaagct gagtctcata ctggggtgta ttggcggcat tgatggaggc atccttcatg
     3181 aaaaaattca ggttggtctg tagccctccg gataccttgc cgccggtaat tccctgtcca
     3241 tatccctgcc ataacaccag gaaaaaaccg aaaaaggtca acgctaattt ttgcatctga
     3301 ggttgaataa aattttaatg tttgctgcaa aagtatgctt ttatttcttc taagccattt
     3361 acaaaacaat aaattattga caaaaaatga agtagctttg cgccgtccat tgagaataaa
     3421 ggggttgttt gtcttaatat acgtgtgaaa attggttaaa gtctagttaa acacactgca
     3481 ttgagtaaaa aataaacgtt ctttatggtt taatagatcg atattttttt gcatttttta
     3541 attaaattaa aaagaaagga tacaatgcgt tttcaaaatc tataccttac tttcatgatg
     3601 ctgtttgtaa ttggtgtaag tactcaggct ttcggtcaga ccgatctccc atcgacccgt
     3661 gtcaagacac tcgacggcaa aagtgtgact cttcaggaag taatcaagcc gggagtcatc
     3721 acagtcattt cgttttgggc cacatggtgc gcaccatgca agaaagagtt ggatgccatc
     3781 aatgagatat atgaggactg gcagagtgaa tacggggttg agctaatcgc catttcaacc
     3841 gatgatgcac gtactgcacc taagatcaag ccgaccataa cccaaaaagg ctggaaatat
     3901 accttcctca atgatcaaaa tcaggaattg cagaaagcac tcaattttca ggcagttccc
     3961 cagactttcc tgataggtaa ggatggtaag atcgcctgga cgcatgcagg atatgtaccg
     4021 ggtgatgaaa atgaactgga aaaccatatt aaggaattag ccggaaagta accttgtatt
     4081 gatgaataaa ttattacatc ccgggcttag gaagaagcct tttttc
//
LOCUS       DAOQMC010000211         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10688_length_4125_cov_5.457740, whole genome shotgun sequence.
ACCESSION   DAOQMC010000211 DAOQMC010000000
VERSION     DAOQMC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10688_length_4125_cov_5.457740"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKV31_08195"
     CDS             complement(<1..399)
                     /locus_tag="PKV31_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNB92878.1"
                     /translation="MKRFINTLKNIWAIKELRNRILYTLLLLLVFRVGSFIVLPGVAS
                     GVLTDVANSRSANDLLGLINIFTGGAFNQASIMALGIMPYITASIIIQVLGFAVPYFQ
                     RLQQKEGESGRKKLNQITRVLTVAITLVQGG"
     gene            complement(453..899)
                     /gene="rplO"
                     /locus_tag="PKV31_08200"
     CDS             complement(453..899)
                     /gene="rplO"
                     /locus_tag="PKV31_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNB92879.1"
                     /translation="MELNNLRPAKGSNKSGKRIARGQGSGRGGTATRGHKGDKARTGF
                     KNKRAHEGGQMPLQMRLPKFGFKNVNRVDYVGINVSTLEALAEIHNVTEINFDFLTSK
                     KVIKKSEKFKILGAGELSKALTVSAHSASKAAQDKITAAGGTFTTI"
     gene            complement(928..1110)
                     /gene="rpmD"
                     /locus_tag="PKV31_08205"
     CDS             complement(928..1110)
                     /gene="rpmD"
                     /locus_tag="PKV31_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNB92880.1"
                     /translation="MGKLKITQVKSTIDRNKRQKATIEALGIKKVYRSVVHEDTPAIQ
                     GMIRAVAHLVKVEEVK"
     gene            complement(1118..1624)
                     /gene="rpsE"
                     /locus_tag="PKV31_08210"
     CDS             complement(1118..1624)
                     /gene="rpsE"
                     /locus_tag="PKV31_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNB92881.1"
                     /translation="MRPVDAELKDKLVVVNRVAKVTKGGRTFSFAAIVVVGDGQGTVG
                     QGLGKARDVSDAIAKAIDDAKKNLITFPILKGTIPHPQKGKFGAGKVLIRPAADGTGV
                     IAGGSMRAVLEIAGVHNVLAKSQGSSNPHNVVKATIDALSKLRDPHTVAQQRGITVKE
                     LFSGEFEK"
     gene            complement(1668..2018)
                     /gene="rplR"
                     /locus_tag="PKV31_08215"
     CDS             complement(1668..2018)
                     /gene="rplR"
                     /locus_tag="PKV31_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNB92882.1"
                     /translation="MKKTKEESRSRIHYRIRKKVAGSAERPRLSVFRSNKAIYAQLVD
                     DTNGNVLTSASSALLETKSDNKVEQAKAVGKLIAEKAKDKNIDSVVFDRSGYLYHGRV
                     KALAEGAREGGLNF"
     gene            complement(2048..2605)
                     /gene="rplF"
                     /locus_tag="PKV31_08220"
     CDS             complement(2048..2605)
                     /gene="rplF"
                     /locus_tag="PKV31_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNB92883.1"
                     /translation="MSRIGKAPITIPKGVEFNVDKSNVITVKGPNGTLTRKFDADMIF
                     KTEDGVLTVERPTDSKRHRSVHGLSRALLNGMVEGVSSGFTKKMELVGVGYRANNTGQ
                     LLELALGFSHPIMFQLPDEVKVETKNEKGMPPTIILNSFDKQLIGQVAAKIRSFKKPE
                     PYKGKGVRFAGEVLRRKAGKTAGKK"
     gene            complement(2635..3039)
                     /gene="rpsH"
                     /locus_tag="PKV31_08225"
     CDS             complement(2635..3039)
                     /gene="rpsH"
                     /locus_tag="PKV31_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNB92884.1"
                     /translation="MALNDPIADYLTRIRNAQQAGHRIVEIPSSKMKKKITEILYENG
                     YILKYTFDDAAGKQGIIKIALKYNEATKKPVIQELKRVSRPGLRQYRHCEELPRVING
                     LGIAIISTSHGLLTDKQARAQNVGGEVVCFIS"
     gene            complement(3084..3353)
                     /gene="rpsN"
                     /locus_tag="PKV31_08230"
     CDS             complement(3084..3353)
                     /gene="rpsN"
                     /locus_tag="PKV31_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNB92885.1"
                     /translation="MARKSVIARERKRERMVAKYAEKRKQLKEAGDYDALDKMPRNSN
                     SIRLHNRCKLTGRPKGYMRDFGICRVMFRKMALDGKIPGITKSSW"
     gene            complement(3379..3927)
                     /gene="rplE"
                     /locus_tag="PKV31_08235"
     CDS             complement(3379..3927)
                     /gene="rplE"
                     /locus_tag="PKV31_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNB92886.1"
                     /translation="MSYIPRYKTKYKDEVVPKLMEEFKYTSVMQVPRLEKICINQGLG
                     DAVADKKVVDNAQADLTTISGQKAVVTKARKSVSNFKLREGMPIGVRVTLRNNRMYEF
                     LDRLISISLPRVRDFKGVNDKSFDGRGNYTMGITEQIIFPEIDIDKIAKVLGMDITFV
                     TTAKTDKEAHALLSALGIPFKK"
     gene            complement(3924..>4125)
                     /locus_tag="PKV31_08240"
     CDS             complement(3924..>4125)
                     /locus_tag="PKV31_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNB92887.1"
                     /translation="EGRNIARKHTKPQQDQPGGIEDVALPIHISNLMVVDKKGNPSRI
                     GRKEEDGKLVRYFKKSGETIKR"
BASE COUNT         1043 a          975 c          754 g         1353 t
ORIGIN      
        1 accaccctgc accaatgtaa tagcaacggt caatactctg gtaatttgat tcaacttctt
       61 tctaccggat tcaccttcct tctgctgcaa acgctggaaa tagggaacgg caaaacccaa
      121 aacctgaata ataatcgagg ctgtgatata aggcatgata ccgagggcca tgattgaagc
      181 ctggttgaag gctccaccgg taaagatatt aattaatcct aacaggtcat tagcgcttct
      241 gctattcgcg acatcggtca acactcctga tgcgacaccc ggcaatacta taaaagaccc
      301 cactctaaag accaaaagca gtagaagcgt ataaagaata cgatttctta gctccttgat
      361 ggcccaaata ttttttaaag tgttgataaa tcttttcatc cttaatctta atatttaaat
      421 gatctcccta aggttattca gggcttcgta tattaaatgg ttgtgaatgt tcctccggca
      481 gcggtaattt tatcctgtgc agctttggag gcagaatgtg ctgatacggt tagtgctttg
      541 gacaactccc ctgcacccag aatcttgaac ttctcagact tctttattac cttcttagag
      601 gtcagaaaat caaagttgat ctcagttaca ttgtgtattt cagccaaagc ctccagtgtg
      661 cttacgttga taccgacata atccacccga ttgacattct tgaaaccgaa cttaggaagt
      721 cgcatctgaa gaggcatctg tccaccttca tgagcgcgct tgtttttgaa acctgttcgt
      781 gccttatctc ccttatgacc acgagtggca gtaccgcctc taccgcttcc ctgccctctg
      841 gcaattcttt tacctgactt gttcgaacct tttgccggtc ttagattatt taattccatc
      901 ggaataattt acttaattat gtaaatatta ttttacttct tccactttca ccaggtgagc
      961 taccgctctg atcataccct ggatggcagg tgtatcttcg tggactaccg aacggtaaac
     1021 cttcttgata cctaatgctt caatggttgc tttttgtctt ttattacggt caattgtact
     1081 tttgacctga gttattttca actttcccat gattcaatta tttttcaaat tcaccactga
     1141 acaattcctt gacggtgatt cctctttgct gagcaaccgt atgaggatct cttaatttgg
     1201 ataatgcatc aatcgttgcc tttacaacgt tatgaggatt ggagctaccc tgtgacttgg
     1261 ccaatacgtt gtgtactcct gcgatctcca gtacagctct catggaacct ccggcgatca
     1321 caccggtacc atctgctgca ggtctgatca acacctttcc tgctccaaat ttcccctttt
     1381 gaggatgggg tatggtacct ttcagtattg ggaaggtaat cagatttttc ttggcatcgt
     1441 caatcgcctt ggcaattgca tcagatacat cccttgcctt acccagtcct tgtccgaccg
     1501 taccttgtcc atcacctacc actacgatgg cagcaaagct aaatgtacgt ccaccctttg
     1561 ttacttttgc aacccggttg acaactacaa gtttgtcctt caattcggcg tcaactggac
     1621 gcattctatt ttgtggttgt tttgccatta ttctttagct aataaattta aaaattcaaa
     1681 ccgccttcgc gagctccttc tgctaatgct ttcactctac cgtgatacaa ataaccgcta
     1741 cggtcaaaaa ccaccgagtc gatattctta tccttggctt tttctgcaat caatttgcca
     1801 acagccttag cctgttcgac cttattatct gatttagttt ccaacagagc agaagacgct
     1861 gaagtcaata cattaccatt ggtgtcatca accaactgag catagatagc tttgttgcta
     1921 cgaaacacgc ttaatcgtgg tcgttcagct gaacctgcaa ctttctttct gattctgtaa
     1981 tgaatccgcg atctggattc ttcttttgtc tttttcatat ctaatgatat agagaattaa
     2041 acggatttta tttcttacct gcagtttttc cagcttttct gcgtaaaact tctccggcaa
     2101 atcgaacacc ctttccttta tagggttcag gtttcttaaa tgagcgaatc ttggctgcaa
     2161 cctgaccgat caattgcttg tcaaagctat taagtatgat agtaggtggc atacccttct
     2221 cattttttgt ctccactttc acttcatcag gaagttggaa catgatagga tgtgagaaac
     2281 cgagtgcaag ctcaagtagt tgtccggtat tattcgctct gtaaccgaca cctacaagtt
     2341 ccattttctt ggtaaaaccg ctagatacgc cttccaccat accattgaga agggcacggc
     2401 tcagtccgtg aacagagcgg tgacgcttgg agtcggtagg tctttcaaca gtcaatactc
     2461 catcttcagt cttaaagatc atatcagcgt caaacttcct ggtaagggta ccattaggac
     2521 ctttaacagt aattacattt gacttatcaa cattaaactc cactcctttc gggatcgtaa
     2581 tgggagcttt accaattctt gacataatta attatttctt tctttctcaa aaaattagct
     2641 aataaagcaa acaacttcac caccgacatt ctgtgccctt gcctgcttat cagtcagcag
     2701 accgtgtgat gtagagatga tagcgatacc cagaccattg ataactcgtg gtaattcttc
     2761 gcagtgacga tattgtcgca atcccggtcg acttaccctc ttgagctcct gaataacagg
     2821 ctttttagtt gcctcattat atttaagtgc aatctttata ataccttgct taccggcagc
     2881 atcatcaaag gtatacttca ggatatagcc attttcatac aggatttcag tgattttctt
     2941 cttcattttg gaagagggaa tctccacaat tctgtgtccg gcttgttgag cgtttctgat
     3001 tctggtcagg taatctgcta ttggatcatt cagagccatt ctattataat ttagtttgct
     3061 ataattaatt tatgggaata ttgttaccag gaggatttcg ttataccagg aatcttgcca
     3121 tccagagcca tttttctaaa cattaccctg cagataccga agtcacgcat ataacctttt
     3181 ggtcgaccgg tcaatttgca acggttgtgc aacctgatcg aattagaatt gcgtggcatc
     3241 ttgtccaaag catcataatc tcctgcttct ttcagttgtt ttcttttctc ggcgtattta
     3301 gcaaccatgc gttctctttt tcgctcgcgt gctataactg attttcttgc catcctgaaa
     3361 tattatttgt ctttattatt attttttaaa tggaattccc aatgctgaca acaaagcgtg
     3421 agcttcctta tccgtctttg ctgtagtaac gaatgtgata tccataccaa gtactttggc
     3481 gatcttatct atgtctatct ccggaaagat aatctgttca gtgatgccca tagtatagtt
     3541 accacggcca tcgaaactct tgtcattgac tcctttgaag tccctaaccc gaggcaggga
     3601 tattgaaata agacgatcca ggaattcgta cattctattg ttgcgcaatg tcacgcgtac
     3661 accgatcggc ataccttctc tcaacttaaa gttggagacg gatttgcggg ctttagtaac
     3721 aaccgccttt tgtcctgaaa tggttgtcag gtcagcctga gcattatcaa ccaccttctt
     3781 atctgccacc gcatcaccca atccttggtt gatacatatc ttttcaagac gaggcacctg
     3841 catcactgat gtatatttga attcctccat cagttttgga acaacttcgt ccttatactt
     3901 ggtcttatat cgtggaatat aactcatctt ttaatagttt ctcctgattt tttaaaatat
     3961 cttaccaatt tgccatcctc ttccttacgg ccaattctgc tggggttgcc tttcttgtcc
     4021 acaaccataa ggttggagat atgaataggc agagcgacat cttcgatacc gcccggctgg
     4081 tcctgctgag gcttagtatg ttttctggct atattgcgtc cttcg
//
LOCUS       DAOQMC010000212         4121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10707_length_4121_cov_4.551648, whole genome shotgun sequence.
ACCESSION   DAOQMC010000212 DAOQMC010000000
VERSION     DAOQMC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10707_length_4121_cov_4.551648"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..2461
                     /gene="dnaK"
                     /locus_tag="PKV31_08245"
     CDS             542..2461
                     /gene="dnaK"
                     /locus_tag="PKV31_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNB92888.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNDPVVIPNDEGARTTPSVVAFMD
                     NGERKIGAPAKRQAITNPQRTIASIKRFMGHRFSEVGADVEHSAYKVIKGDNDTVRVE
                     IDGRFYTPQEISAMILQKMKKTAEEFLGQEVTEAVVTVPAYFNDSQRQATKEAGEIAG
                     LNIKRIINEPTAAALAYGLDKKHKDSIVAVYDLGGGTFDISILELGDGVFEVKSTNGD
                     TQLGGDDFDKKIIDWLADGFKAQENIDLRKDPMALQRLKEAAEKAKIELSSGNETEIN
                     LPYVTAIDGVPKHLVMKLNRSKFEQLTDDLVSRSIEPCRQALKDAGLTTNDIDEVILV
                     GGSTRIPKIQEAVEQFFGKKPNKGVNPDEVVAIGAAIQGGVLSGDVKDVLLLDVTPLS
                     MGIETMGGVYDVVIEANSTIPTKKSKIYSTAADNQPSVEIHILQGERPMAKDNRSIGR
                     FILGDIPAAPRGTPQIEVTFDIDANGILQVTSKDKGTGKQQSIRIEASTGLSKEEIER
                     MRTEAAANADADKKAKERIEKLNGADSMIFQSEKQLKEYGDKISAGNKTAIEAALNDL
                     RDAHKTENFDKIDSASAALNNAWQAASQEMYQAGQTGADPGAGAGTESNHSHNNSNGA
                     SEDVTDVEFEEVDKN"
     gene            2696..3124
                     /locus_tag="PKV31_08250"
     CDS             2696..3124
                     /locus_tag="PKV31_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HNB92889.1"
                     /translation="MYWYLEVFSRYADFSGRSRRKEYWMFILFNMLFLFGIFAIMTAS
                     LAIFGDSRIVTLLLGIVMIIYVLIVFIPSLAVTVRRLHDTGKSGWYILLNLIPYVGGL
                     IIFIFTVMDSEPGENKWGPNPKEDYLYDSEIDEIGMKDEY"
     gene            complement(3142..>4121)
                     /locus_tag="PKV31_08255"
     CDS             complement(3142..>4121)
                     /locus_tag="PKV31_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB92890.1"
                     /translation="TSKTMVYKMRPRERGVFSFGALNIFVTSAFSLVSRRYIFDADKE
                     VPVYPSYIHLDRYDIRRIGNDLNFPGIKKVRRIGHSMEFEQIKEYVAGDDWRSVNWKA
                     TSKTAKLMVNQYMEEKAQPVYCLIDTGRMMKMPFDGLTLLDHCINSVLVLSHVVIGNQ
                     DKAGLATFSRKLENFLPAERRTGQMHLILEKLYRIRTDYKESDFGRLYNDIRRLINQR
                     SLLLLYTNFDTIDALRRQLKYLRGIAKGHVLLIIFFKNTEMEAIKYEEALDIREIYDR
                     AVVEKFNFEQRLIVNELRKYGIYSILTEPRYLTVDLINKYLEVKARGLI"
BASE COUNT         1290 a          863 c          835 g         1133 t
ORIGIN      
        1 actctctact gacgcgcttc ctgcaaaata ctggttaaac ttttgttcat cacaacgcgt
       61 atttcaaatt gcgatcgccg gatatgccgc ttcacacagt aagggaagct attaatcatc
      121 tacctacttc gttgcggacc gtgaaatcag tagggtttca catcgtcggc agcaatttgg
      181 aaaaggaaaa atcgaagtag gtaggaaagt aatagtcatg aactccctat tgatgcacct
      241 cctgcaaaac actggttaaa tttttgttca tcacaacgcg tatttcaaat tgcaatcgcc
      301 gaaaatgcct cctcacacag taagggaagt tattaatcat ctacctactt cgttgcggac
      361 ctcattccca tttaccaccc tatattccaa ctgccaatat ttcacatatt ctaaacattc
      421 cactcaaatt tggcaccttc cctcattcgc tcaccactta atatgacatt ttgtcggtca
      481 gattcgaatg gcacactagt tgactatatc ggattaaatt tataattcaa aaacaataga
      541 tatgggaaaa ataataggaa ttgacttagg aacaaccaac tcctgtgttt ccgtaatgga
      601 aggtaacgac cctgtcgtaa taccaaacga cgaaggtgca aggactactc cttcagtagt
      661 tgcctttatg gacaatggag aaagaaaaat cggtgcacca gctaaaaggc aggcaatcac
      721 caaccctcag aggactattg cctccattaa aagattcatg ggtcatagat tctctgaagt
      781 aggtgcggat gtagaacatt ccgcctacaa agtaataaaa ggagacaatg acacagtgcg
      841 agtagaaatt gatggcagat tttatacccc acaggaaatt tcagccatga ttcttcagaa
      901 aatgaaaaag acagctgaag aattcctggg ccaggaagtt acagaagcag tggttactgt
      961 acctgcttat ttcaatgact cacagcgcca ggctaccaaa gaagcgggcg aaattgcagg
     1021 ccttaacatt aagcgaatca tcaatgaacc tactgccgct gcattggcat acggccttga
     1081 taagaaacat aaggattcta ttgtggctgt gtatgacctt gggggaggta ctttcgatat
     1141 atccatcctt gaactgggcg atggtgtatt tgaagtaaaa tcaaccaatg gagatactca
     1201 attgggaggt gatgatttcg ataaaaaaat tatcgattgg ttagctgacg gttttaaggc
     1261 gcaggaaaat attgacctta gaaaagaccc tatggcactt cagcgcttga aggaagctgc
     1321 agaaaaggcc aaaatcgaat tatcctccgg gaatgagaca gaaatcaacc tgccttatgt
     1381 tacagcaatt gatggtgttc ctaaacacct tgtaatgaaa ctcaacagat ctaaattcga
     1441 acaattgact gatgatctgg taagcagaag tatagaaccg tgccgtcaag cattgaagga
     1501 tgcaggtcta actaccaatg atatagacga agtgattctt gtcggtggat caaccagaat
     1561 tcctaagatc caggaggctg ttgagcaatt tttcggtaaa aaaccgaata aaggagtcaa
     1621 tccggacgag gttgtagcaa tcggggctgc aattcagggg ggtgtcttga gtggagatgt
     1681 taaggatgta ttgctactcg atgtaacgcc cttatccatg ggaatcgaga caatgggagg
     1741 agtatatgat gtggttattg aagccaactc aaccattcct accaagaaat ccaaaatata
     1801 ctcaaccgca gcagacaatc aaccctctgt agaaatccac attctccagg gagaaaggcc
     1861 aatggcaaag gataacagat ctatcggaag gtttatcctt ggtgatattc cagctgcccc
     1921 tagaggcact cctcagattg aagtaacctt tgatatcgat gccaacggta tcctccaggt
     1981 aacctcaaag gataaaggta ccggtaagca acaaagcatc cgtatcgaag cttctactgg
     2041 attgtcaaag gaagagattg aaagaatgcg tacagaagct gctgctaatg ctgatgcaga
     2101 taagaaggct aaagagagaa tagagaaact caatggagca gactcaatga tcttccagtc
     2161 tgaaaaacag cttaaggaat atggagacaa aatctctgcc ggtaacaaaa cggctattga
     2221 agcagctctg aatgatctca gagacgctca caaaacagaa aattttgaca agatagattc
     2281 tgcaagtgcc gcactgaaca atgcctggca agctgcaagt caggaaatgt accaagccgg
     2341 tcaaaccgga gctgatcccg gagcaggtgc cggtacggaa agtaaccatt ctcacaacaa
     2401 cagtaatggt gcaagtgaag acgttacaga cgtagaattt gaagaagtgg ataaaaatta
     2461 atccctttta aaggtcatca gcccctactt aacaggctga aactgaacct ttcaattagg
     2521 atattgacgg agagaaatta tcaggataga ataaatgtta tccaattgca atccgtatta
     2581 taccccataa attaaaaaaa ggccgggctt atcagtccgg ccttttttta tattttatgg
     2641 cgataaggag aattgttaat gatttattta ttatttttga agaaaaatat ttgttatgta
     2701 ttggtatttg gaagtattct caagatacgc tgacttttca ggcagatcca gaagaaagga
     2761 atattggatg ttcatactat tcaacatgct ttttttattc ggcatttttg ctattatgac
     2821 tgcttcatta gccattttcg gtgacagtag aatcgttacc ctccttttgg gcatcgtgat
     2881 gataatatat gttttaatcg tttttatacc ttctctggcc gtgaccgtaa gacgccttca
     2941 tgataccggt aaaagtggat ggtacattct tctgaacctt attccttatg taggtggatt
     3001 gataatattt atttttactg ttatggacag cgaacctggt gagaataaat ggggacctaa
     3061 cccaaaagaa gactacctgt atgattctga aattgatgaa atcggaatga aggatgagta
     3121 ttgaccattg ttgggctcaa tttatatcag tcctctggcc tttacttcca agtatttgtt
     3181 gatcaagtca actgttaggt acctgggctc tgttagtatg gaataaatgc catatttacg
     3241 caattcgttg acaatcagtc tctgttcgaa attgaacttt tcaaccactg ccctgtcata
     3301 aatttcacgg atatcaagtg cttcttcata cttgattgct tccatctccg tatttttaaa
     3361 aaaaatgatc agcaaaacat gccctttggc aataccgcgc aaatacttca actggcgccg
     3421 aagtgcatct atcgtatcaa aattggtata caacaaaagc aaacttctct gattgatcag
     3481 acgccgaata tcattgtaca accttccaaa atcagactcc ttataatcag ttctaatccg
     3541 atagagcttc tccagaatta aatgcatctg ccctgttctt cgttctgccg gcaggaaatt
     3601 ttccaatttc ctgctaaaag tcgctaatcc ggccttgtcc tgattaccta ttacaacatg
     3661 gcttagtact aagacgctat tgatacagtg atccagcaga gtaagtccat caaacggcat
     3721 tttcatcatc ctacccgtat caatcagaca atataccggt tgcgccttct cttccatata
     3781 ttgattgacc atgagtttgg ctgtcttgga agtagccttc cagttgactg accgccaatc
     3841 atctccagcc acatattcct tgatctgctc aaactccata ctatggccta tcctcctcac
     3901 cttctttatt ccgggaaaat taagatcatt gcctatcctc ctgatgtcgt acctgtccag
     3961 atggatatat gaagggtata cagggacttc cttatctgcg tcaaatatat atcgtcgact
     4021 tacaaggcta aatgcagaag taacgaaaat attcaaagcc ccgaaagaga acacaccacg
     4081 ctcccgggga cgcattttat acaccattgt tttggatgtc c
//
LOCUS       DAOQMC010000213         4064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_10984_length_4064_cov_4.871040, whole genome shotgun sequence.
ACCESSION   DAOQMC010000213 DAOQMC010000000
VERSION     DAOQMC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10984_length_4064_cov_4.871040"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PKV31_08260"
     CDS             <1..974
                     /locus_tag="PKV31_08260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB92891.1"
                     /translation="QSIRQITTDEHDYSAIIGVGADGKIIVQRTDYNHAVEIFSVDPS
                     TGKATQLTHVNDDLYKTISMSKVEKKIIKTTDGKDMVAWVIYPPNFDPNKKYPTLLYC
                     QGGPQSALTQFYSFRWNMQLMAANGYIVIAPNRRGMPGYGQEWNEAISGDWGGQPIRD
                     YLSAADAMAAEPYVDKNRMGAVGASYGGYSVYMLAGKHEKRFKTFISHCGLFDMDSWY
                     GSTEELWFANFDLKGPYWQKPQPKSYTDFNPIKFVDRWDTPILIFEGEKDYRVPYTQG
                     LEAYQAAQLRGIKSRLVIYPDEGHWVTKPFNSLVWHHEFFRWLRETL"
     gene            complement(1245..2507)
                     /locus_tag="PKV31_08265"
     CDS             complement(1245..2507)
                     /locus_tag="PKV31_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92892.1"
                     /translation="MRDRIINSLLFLFIFYSAGVAQSISSSHAVMDAAVTGKIINRES
                     VLLGKKINLQTDQNTYPDIELNGDGTFAVELPENFPLRYRLFDDKDFLNGINVGDIIK
                     IHSYILGKNPINDPLSLIAADVNRSGKVTVADIVEIRRLILGKVDQFQSGDSWIFIDA
                     EFPLFINNWELANTYVNPTTDNSFYNEFLAVKVGDVDRIIFSRSDDVYIRDPSSKSIA
                     YLTRDQGRIMLWLDKTARHAEGIQLGLNLDFGLQFTSCNPMLDDDQYSFSEDGKSVKI
                     VLLNDEIEALYDKPLFIFDYYTLNNDQEPIRLSEDFENMIISNGESFSVAPIGFKKLS
                     VSASYPSPNPFRDNTVISFKSEVVQDVKLEVYDLNGCKIYNQNINALPGQNTVSLTRT
                     ALKERAGVYYYNLFGKDIHSQGRIIMLD"
     gene            complement(3068..4027)
                     /locus_tag="PKV31_08270"
     CDS             complement(3068..4027)
                     /locus_tag="PKV31_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92893.1"
                     /translation="MIPAIPGYSVEAWKSFVFNPGRLRRMQEGAGIILDFLQLVLGQG
                     LLASELLSEDNLKELSTRLVDTQIPGAARKIKSFARYELNPETLPMFRFELRYLGNLA
                     HMLMGFEQLSLSAKLQVWQICGGLISKELVLNQPGVNDRWVTKSVVVAREDGLVTRKT
                     WFYGTHTLMWAFTLDYSFGTQPLPEGNKVGDTFNGTAKFYPGLLPGRILFTEKAEDSG
                     VYRTPDVMRFEDIARLKLGVARLAAHDPLFHEYPVLMDKIILTSNNGQFYLVDVNNEF
                     LPLITVEKRGYGSKDDAWERFADAGGHPVSLWVMVSQGQFILI"
BASE COUNT         1268 a          905 c          729 g         1162 t
ORIGIN      
        1 agcaaagcat cagacagatt acaactgacg agcatgatta ttcagccatc ataggtgtgg
       61 gtgctgatgg taagatcatt gttcaacgta ctgactacaa tcacgctgtc gaaatttttt
      121 cggtagatcc ctcaaccgga aaagcaacac agctaacaca tgttaatgat gatttgtaca
      181 agactatcag catgagcaag gttgaaaaga agatcatcaa gacaacggac ggaaaggaca
      241 tggtcgcctg ggttatttat ccaccaaact ttgatcccaa taaaaaatac ccaacattac
      301 tgtactgcca gggaggtcca cagagtgctc tgacacaatt ctattctttc agatggaaca
      361 tgcagcttat ggcagccaac ggatacattg tcatagctcc gaaccgtcgg ggaatgcctg
      421 gttatggcca ggaatggaat gaagccattt caggtgattg gggtggacaa ccgattagag
      481 attatctaag tgcagctgat gccatggctg cagagccgta tgttgataaa aacagaatgg
      541 gagcagtagg cgcatcttac ggaggatatt ctgtgtatat gctcgccgga aagcatgaaa
      601 aacgattcaa aacctttatc tcacattgtg gacttttcga tatggatagc tggtatggtt
      661 ctacagaaga actatggttt gccaacttcg acctgaaggg tccctattgg caaaaacctc
      721 agcccaagtc atataccgac ttcaacccaa taaaattcgt ggaccgatgg gatacaccaa
      781 tattaatatt tgaaggagag aaggactatc gcgtacctta tacccaggga ttggaggctt
      841 atcaggctgc acaactcaga ggaattaaat caagactggt aatctatccc gatgaaggcc
      901 attgggtcac aaaacccttc aatagcctgg tgtggcacca tgagtttttt cgatggctta
      961 gagagacttt gtaatgccca ccttgataaa ggtctgtacc cgatctgaat agctctggta
     1021 aagaacctga tactatcaac cgcaataaaa aagtaaagag actatccaac aaggcagata
     1081 gtctcttttc attttccgga aaatggtaaa cagtagtagt atcccttctc aataaatgaa
     1141 tcgtataaaa aaaggccatc cttttaagac agcctctttt tgtccaaatt cataataatt
     1201 aaaacctacc taatccgttg cttccgaaac gggtatttct tcaatcaatc cagcattata
     1261 atccttccct ggctatggat gtctttcccg aaaagattgt agtaatagac tcccgctctt
     1321 tctttcaacg ctgttcgggt taaagaaaca gtgttctgtc cgggcagagc attgatattt
     1381 tgattgtata ttttacatcc attcagatca tagacctcta gtttcacatc ctgaacaact
     1441 tctgacttaa aactaattac cgtattatcc ctaaaaggat tggggctcgg ataacttgct
     1501 gaaacagaaa gcttcttaaa tccaatcggt gcaacggaaa aggattcgcc attcgatatg
     1561 atcatgtttt caaaatcttc tgacagccta ataggctcct gatcattatt caatgtatag
     1621 taatcaaaaa taaatagcgg cttatcatat aatgcctcta tttcatcatt taatagcact
     1681 atcttaacac ttttgccatc ttcactgaaa gaatactgat catcatcaag cattggattg
     1741 caggaagtaa attgcagtcc gaaatccaga ttcagcccca attgaatacc ttcagcatgc
     1801 cttgccgttt tatccaacca aagcattatc ctcccctgat cacgtgtcag ataagcgatt
     1861 gacttacttg aaggatctct gatatagaca tcatcacttc gactaaatat aattctatcg
     1921 acatcgccaa cctttacagc cagaaattca ttgtaaaaac tattatctgt agtcggatta
     1981 acataggtat tggccaactc ccaattatta atgaatagcg gaaattctgc atcgataaat
     2041 atccatgaat ctccggattg aaattgatct acctttccca atatcaatct tcttatctca
     2101 acgatatcgg caacggtcac cttcccactc ctgtttacat ctgcagcaat caggcttaac
     2161 ggatcgttta ttgggttctt acctaaaatg taggaatgaa tcttgatgat atctcctaca
     2221 ttaataccat tcagaaagtc cttatcgtca aacaacctat accgaagtgg aaaattctcc
     2281 ggcagctcaa ctgcaaacgt tccatcacca ttcaattcaa tatcagggta agtattctgg
     2341 tcagtttgaa gattgatctt ctttccaagt aatacactct cccgattgat tattttaccg
     2401 gtcactgctg catccataac tgcatgtgaa gaggaaatag attgtgcaac tcctgctgaa
     2461 taaaaaataa acaggaataa caggctattt ataattctat ctctcatagt gaatatttcc
     2521 aatgcacgat agcagctatt tctaagattt atcaggaaca aatgattttc catatcagta
     2581 agattaaaat cattgatttt ctattgagaa cgaatgaaac actcaggcaa tgccttcgaa
     2641 ttgttatttc atcagtctgt aaaagatcta tcttgactgg tttgaggatg ccaccttgac
     2701 cagatctccg ataggagacc gataccaggc gttaaattct tataccattg tgaccagtca
     2761 ttacttgcac cgacccacag atctaatacg gccttaaccg tattagaacc atggataaaa
     2821 ttcatacctt taaagataag caacagagca gagtttcctt tgaaatctga tggaactatt
     2881 aatgccccaa aagcatgaag tggtaaagtt taaaaacctt aatacaaaaa tcaaacgagt
     2941 aaattctata agacattact ccttgacgat cccatccgtt gcctcaaatt tgatttttta
     3001 ttttaatatt tattgtgtta atatatataa atctgtaata gtatttatta tatattaaat
     3061 attttaatta aattaaaata aattgccctt gggaaaccat cacccacagg ctgaccggat
     3121 gccccccggc atcagcaaac cgctcccagg catcatcctt gctaccatat cctcttttct
     3181 ccacagttat caatggcaaa aattcattat tcacatccac taaataaaac tgtccattat
     3241 tgctggtcaa tattatctta tccatcaaaa ccggatactc atggaacaat ggatcatggg
     3301 cagccaatct ggctactccg agtttcaacc ttgcaatatc ttcaaaacgc atcacatcag
     3361 gtgtccgata tacaccggaa tcttctgctt tctctgtaaa aagtattcgg cccggtaaca
     3421 atcccggata aaatttggct gttccgttaa acgtatctcc tactttattc ccctcaggca
     3481 ggggttgtgt cccgaagctg taatcaagag taaaagccca catcaaggta tgcgtgccat
     3541 agaaccatgt ttttcttgtg acaaggccat cttctcttgc aactacaaca ctcttagtca
     3601 cccagcgatc attaacaccg ggttgattca gaacaagttc cttactgatc agcccgccac
     3661 agatctgcca aacttgtagt tttgctgaca aagataattg ctcgaagccc atcaacatat
     3721 gtgcgagatt gcccaggtat ctcaactcaa aacgaaacat aggcaatgtc tccggattga
     3781 gttcataacg agcgaatgac tttattttgc gagccgctcc gggaatttgc gtatcgacca
     3841 gtctggtgga taattctttc aaattatctt cagaaagcaa ttcactcgct aaaagtcctt
     3901 gccctaatac taattgaaga aaatccagta tgatgcctgc tccctcctgc attctacgga
     3961 gtcggccggg gttaaaaaca aagctcttcc aggcttcaac tgaatatccc gggatggcgg
     4021 gtatcacaac aggctcgttt cagcatcctc cagtgagagg atac
//
LOCUS       DAOQMC010000214         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_11004_length_4060_cov_5.335331, whole genome shotgun sequence.
ACCESSION   DAOQMC010000214 DAOQMC010000000
VERSION     DAOQMC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11004_length_4060_cov_5.335331"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="PKV31_08275"
     CDS             complement(<1..1774)
                     /locus_tag="PKV31_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNB92894.1"
                     /translation="MKIFTSKMFVLAVMAGLVLGLTAWVDDPPDFHTGAPGELTCASC
                     HVPPSVNPSVGTLEIIGLPDSLQPATEYRFTVRMTKTSGDAIQAGFQMTLVNERDVKF
                     GVFSNPSPKSAISVRPYRDYFEHRTPGSFATGNVIEWNVSWTSPPADEGFSQVLRLYA
                     SGLMSNADSSITGDVTLNVNETIRYFSPYKRLEGGITGTNITCSGAKDGTATASATGG
                     KPPYKYNWNTGDTTETVDNLMAGTYSLTITDQGTDTIVKIVTLTQPFPVFGTIIKTDI
                     PCNSSVAGEAIVVPNSGIPPYSFLWSNGDTTTTTSFSKHGPISVVITDSNGCSYTAHS
                     SIIKKGDFTASISEVTNVKCFGGSTGTARVTTSIPFPTSFTWSNGASTQYVEGLAAGT
                     YSVTVTDVDGCEARASAEVLQPTAIQVKKNQSNQPSCRGSFNGYISVFADGGVPPYSF
                     AWEDGTNLKTLANLTAGDYTVTVTDAFGCTSSGTFVLTEPDEIDILVDKNNESASNAN
                     NGKALILPSGGSIPYTYLWSDGVTSRDRTGMAPGYYTVTVTDVKGCTAETGLYIEPFE
                     CGLVVTGVEVHDVECNGTAGGYIHL"
     gene            2153..3247
                     /locus_tag="PKV31_08280"
     CDS             2153..3247
                     /locus_tag="PKV31_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNB92895.1"
                     /translation="MKFLKILDWFIIKKYLTTFFFTAFIFTMIAVVIDTSDKLEKFLR
                     EECTLQEIIFQYYGAFIPHINWLLWPLFSLISVIFFTSRMAANSEVISILNAGVSYNR
                     FLRPYFISAGFICLVHAIGNHFFIPILNKNRIDFEAQYIKKVDNQNQNNEVHLMLSAD
                     SKVFVRFYNRADSTARDLRMETFKNGNLVEIYKAREAKFLGNPNQWLLTGLERHTFDS
                     LQETIRIQPRDTVRMSLNIGPDDFYTIRNFKETLPSPALLRFIKKEESRGTGVAGEYR
                     IEFHRRTADSATLIILMLIGVSVASRKVRGGMGMNLAFGVSLGAAYMILSRFSVTFAQ
                     SNIISPLLGVWIPNLIFIVVSIWLVSKAQK"
     gene            3404..>4060
                     /locus_tag="PKV31_08285"
     CDS             3404..>4060
                     /locus_tag="PKV31_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNB92896.1"
                     /translation="MNKILSCLLVLLCIYSYGQTIPQWRIADWTGVGIKKPMNDDRVL
                     LRIADFGGLGNGTTSNVIAWDEAISALQGKPGIIEFDQGIYLFDRIISIPSHTILRGK
                     GAESTILRFVYPATGNLISLQGRLDDDVAFLTRDAFRNDDFIILTTTDAFNAGDLVRI
                     SMDDTGLVTSDWAKGTVGQIIEIKDQRHDTLFLSEQLRLDLPLQRQPVIRKIDPVKYA
                     G"
BASE COUNT         1088 a          878 c          900 g         1194 t
ORIGIN      
        1 caagatgaat atatccaccc gcggtgccat tacactcgac atcgtgtacc tcaactccgg
       61 tcaccaccaa cccacactca aatggttcaa tgtatagacc ggtttctgct gtacaccctt
      121 tgacatcggt gacagttact gtatagtaac ccggagccat accggtccga tcacgggaag
      181 ttacaccatc tgaccatagg taggtatatg ggatggagcc gccggaaggt aggatcaacg
      241 cctttccatt atttgcattg gaggcacttt cattgttttt atccactaaa atatcaattt
      301 catccggttc cgtcaacaca aaggttccgg aagaggtgca gccgaaagca tcggtcaccg
      361 taacagtata atctccggcc gtaagattgg caagcgtctt gagatttgtt ccatcctccc
      421 aggcaaatga atacggaggt acgcctccat ctgcaaatac cgaaatataa ccattaaaac
      481 tgccacgaca actgggctgg ttggattggt tctttttaac ctgaattgcc gtcggctgta
      541 gtacttcagc tgaggctctg gcttcacatc catcaacatc tgttactgtc acactgtacg
      601 tccctgctgc aagaccctcg acatattgag tcgatgcacc attagaccag gtaaatgaag
      661 tagggaacgg gatagacgtg gttacgcgag ctgttcctgt ggatcctcca aaacatttca
      721 cattggttac ctctgaaatg gaggcggtaa aatcgccttt cttgatgatt gagctgtgag
      781 ctgtgtagct gcaaccattg gaatcggtta taaccacaga aatcgggcca tgttttgaaa
      841 atgatgtggt agttgtagta tctccattag accaaaggaa actataaggt ggtattccgg
      901 aattcggtac cacgattgct tctcctgcaa cggaagaatt acagggtatg tcggtcttta
      961 tgatggtccc gaacacagga aaaggttgag ttaaagtcac tattttgacg atggtatcag
     1021 ttccttgatc ggtgatggtg aggctatagg tgcctgccat aagattatcg acagtttctg
     1081 ttgtatctcc cgtattccag ttgtatttgt agggtggttt tccacctgtg gcactggcgg
     1141 tagctgttcc atcttttgct ccactgcaag taatattggt tccggtgatg cccccttcta
     1201 gccttttata tggagaaaaa tagcgaattg tttcattgac gttcaaggtt acatctcctg
     1261 ttatactgct gtcggcattg ctcatcagac cactggcata aagtcgcaat acctggctaa
     1321 atccttcatc agccggaggg cttgtccagg aaacattcca ctcaatgaca ttacccgtag
     1381 caaatgatcc gggcgtcctg tgttcgaagt aatcgcgata cggacgcaca gaaattgcag
     1441 attttggtga tggattgctg aatactccga attttacatc cctttcgttg accagagtca
     1501 tctggaatcc cgcctggatt gcatccccac tggttttcgt cattctaacc gtgaagcgat
     1561 attcagtggc gggctgcaat gaatcgggaa gacctataat ctcaagtgtc ccaacagaag
     1621 gattaaccga tggaggaaca tggcatgaag cacaggtcaa ttctcccgga gctccggtat
     1681 gaaagtcagg cggatcatca acccaggcgg ttaaccccaa aacaaggccg gccatgacag
     1741 ccagaacaaa catcttactt gtaaatatct tcataatgaa ggggattttt ttgttcaaag
     1801 taagcaaaga taatcagtcc ttgataaaat tttccaaatt gttgtcatcc tcacttccat
     1861 cattattatc aactattgga atatgcgcca ttgtaaagac tttcgatgaa tgatgtagaa
     1921 taattcaatt tcatactatt tcatataggg gaagagtcat cgatagtagc ggctgaatgc
     1981 ctgtttctga tttcgcctta tttatagcat catggattga tacaactttt ttacctttgg
     2041 gcgttatctt gtcgcttcat gaatatttac aattttgatt ctaataaagt gtcgttggat
     2101 gagcaagccg gacccggaga taattttcat tgctaactgt tataactaaa ggatgaagtt
     2161 tctgaaaatc ctggactggt ttatcattaa gaagtatttg acgaccttct tctttacagc
     2221 gtttatcttt acaatgattg ccgttgtaat tgacacaagt gataaacttg aaaaatttct
     2281 tcgggaagag tgtacacttc aggagatcat cttccaatat tatggagcat ttataccaca
     2341 catcaattgg cttttatggc ccttgttttc actgatcagc gtcattttct tcacttcccg
     2401 aatggcggca aactctgagg taatcagtat tctgaatgcc ggagtgagct ataacaggtt
     2461 tcttcgaccg tatttcattt cggcggggtt catatgtctg gtacatgcga taggcaatca
     2521 cttttttatt cctattctca acaaaaacag gattgacttt gaagcccaat acatcaagaa
     2581 ggttgacaac cagaatcaga ataatgaagt acacctcatg ctttcagctg attccaaggt
     2641 ctttgtgcga ttttacaatc gggcggatag tacggccaga gatttgcgca tggagacttt
     2701 caagaatggc aacctggtag aaatctataa agcccgtgaa gccaaatttc tcggtaatcc
     2761 aaatcaatgg ctgttgacag ggctggagag gcatactttt gacagcttac aggaaaccat
     2821 ccggatccag ccacgagaca cagtgaggat gagcctcaac atcggtccgg atgacttcta
     2881 taccattcgt aattttaagg aaacattacc ttctcctgcc ttgctccgat ttataaagaa
     2941 agaagaatcc cggggcaccg gagtggcagg tgagtatagg attgagttcc acagacgcac
     3001 tgccgatagt gcaactctga ttatattaat gctcatcggt gtatccgtgg cttcccgtaa
     3061 ggtaagaggt gggatgggta tgaaccttgc ctttggcgtt tcccttggcg ctgcctatat
     3121 gatcctttca cggttttctg taacctttgc acaaagtaat attatttctc cacttctggg
     3181 tgtctggatt ccaaatctca tatttattgt agtaagtatt tggctggtaa gcaaggcaca
     3241 gaagtaatgt ataggttgcc gagggatttg tttttggaag cctttgggtt attccggtta
     3301 taatatttat caatctttcc tccgaaaata ttcaccgcta aacatttttc aatcattttt
     3361 atgctgatgt gactatattt ggatattgca tccctttttt cgcatgaata aaatattgtc
     3421 gtgcctgttg gtcctattgt gcatttatag ctatggccag accattccac agtggcgtat
     3481 cgctgattgg acaggtgttg gcatcaaaaa accaatgaat gatgatcggg tgcttcttag
     3541 aattgcagac tttggtgggc tgggaaatgg aaccacatcc aatgtcatcg catgggatga
     3601 agctatatcg gccttacaag gcaaaccggg aatcattgaa ttcgatcaag gcatctatct
     3661 gtttgaccga atcatttcta ttcccagtca taccattctg agaggcaagg gcgcggagtc
     3721 taccatcctg cgatttgtct atccggcaac aggcaatctt atttcacttc aaggcagatt
     3781 ggatgatgac gtagcctttt tgacccggga tgcatttcga aatgatgact ttataatcct
     3841 gacaaccaca gatgctttca atgccggtga tcttgtccgt atatccatgg atgataccgg
     3901 tctggttacc tctgattggg ctaaaggcac ggttgggcag atcatagaaa taaaagacca
     3961 acgtcacgac acgctttttc tatccgaaca gctcagattg gatttgccgt tacaacgaca
     4021 gcccgtgatt agaaagatag atccggtcaa gtatgcaggt
//
LOCUS       DAOQMC010000215         4032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_11112_length_4032_cov_4.662308, whole genome shotgun sequence.
ACCESSION   DAOQMC010000215 DAOQMC010000000
VERSION     DAOQMC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11112_length_4032_cov_4.662308"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKV31_08290"
     CDS             complement(<1..727)
                     /locus_tag="PKV31_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB92897.1"
                     /translation="MRNFLILFLLALSCTIHAQMPFNMMQTKGKGRISGGVVDSISKQ
                     EIEFVSVAVYRQGNSTPVDGALTDKSGMFNIKELKNGVYDVHINFLGYQNKVISNIKV
                     SDSIPDQMLGKIYISPESKLLNEVVVTGEQGLVENKIDRMVYNAGKDISNKGGNAADV
                     LRKVPTLSVDLDGNLSMRGSSSIRVLINGKPSAVMASSVADALKMIPSDEIDKVEVLT
                     SPGAKYDAEGSAGIVNIITKRKTI"
     gene            complement(1465..1650)
                     /locus_tag="PKV31_08295"
     CDS             complement(1465..1650)
                     /locus_tag="PKV31_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92898.1"
                     /translation="MKLIAIIIVTILFVLSVLNFLFRMPTLNITNIELAQVLVIGFFG
                     GALIALILNRVSDSKYF"
     gene            complement(1657..2028)
                     /locus_tag="PKV31_08300"
     CDS             complement(1657..2028)
                     /locus_tag="PKV31_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNB92899.1"
                     /translation="MNKGIITFVLLVLSNILMTFAWYGHLKFKDIAWLNKFGLPGVII
                     ISWLIAFFEYCFMIPANKIGFVENGGPFNLWQLKVMQEVITLIIFTVFTVFLFKNEVF
                     RLNHIIGFTFLILAVFFIFKD"
     gene            complement(2050..2583)
                     /locus_tag="PKV31_08305"
     CDS             complement(2050..2583)
                     /locus_tag="PKV31_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNB92900.1"
                     /translation="MIDYIVFLRGINVNGQNLIKMEDLRKILIDIGLHEPKTYIQSGN
                     IWVKSELSEAESVESIIRKAIRERLGFDVPTLALPQDTLRRIIDNNPFTHEETDKKRL
                     YYTLLAEPPDRVRAEALMEMDTCGDKIVLGNKVVYLLVRDKYSKTKLTNQYIEKILKV
                     KATTRNHATMVKMTETI"
     gene            complement(2584..3147)
                     /gene="pth"
                     /locus_tag="PKV31_08310"
     CDS             complement(2584..3147)
                     /gene="pth"
                     /locus_tag="PKV31_08310"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNB92901.1"
                     /translation="MKYLVAGLGNMGAEYDGTRHNIGFDVADKLVSDAGEKFKHEHLG
                     DLSSIKYKGRTFYILKPSTYMNLSGKALQYWMRKLSIPQDRVIVILDDLNIPFKSIRI
                     RPNGADGGHNGLKNINEVLGNQNYARIRIGIGGDFKRGRQVNFVLGKWNKEEEQDLPE
                     IIAHAAKAVLAFGTLGITETMNQYTRK"
     gene            3437..>4032
                     /locus_tag="PKV31_08315"
     CDS             3437..>4032
                     /locus_tag="PKV31_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNB92902.1"
                     /translation="MGQKLLIVESPAKAKTIEKYLGKDFVVKSSFGHVRDLEKGNNAI
                     DVDNNFTPHYVITPDKVKVVKDLKDISKKSEEVWLATDEDREGEAISWHLCEVLGLNP
                     HTTKRIVFREITKPAIQNAVANPRNLDLDLVNAQQARRVLDRLVGFELSEVLWRKIKA
                     SLSAGRVQSVAVRLVVDREREINNFEVETFFRVKASFPV"
BASE COUNT         1105 a          827 c          821 g         1279 t
ORIGIN      
        1 tgattgtctt gcgtttagtg atgatgttta cgatacccgc gctcccttca gcatcgtatt
       61 tagctcccgg gcttgtgagt acttctacct tgtctatttc atcggaaggt atcatcttaa
      121 gggcgtctgc tacgcttgag gccatgactg cagaaggctt gccattgatc agcacccgaa
      181 tgctgctact tccgcgcata gacagattac catccaggtc aacacttagg gttggtacct
      241 ttctgagtac atcggctgca ttgcctccct tgttggatat atcctttccg gcgttataca
      301 ccatacgatc tattttattc tctactaatc cctgctcccc ggttaccacg acctcattga
      361 gcaacttcga ttccgggctg atgtatatct tgccaagcat ttgatccggg atggaatcac
      421 tcaccttgat attcgaaata actttattct ggtaacccag gaaattgata tgaacatcgt
      481 aaacaccatt tttcaattcc tttatattga acataccact tttgtctgtc agtgctccat
      541 ctaccggtgt ggaatttcct tgacgataga ctgccacaga tacgaattca atttcctgtt
      601 tgctgatgct atcaacaaca ccaccggata tgcgcccctt acctttggtt tgcatcatat
      661 tgaaaggcat ttgggcgtgt attgtgcaac tcaatgccaa caggaagagt atgagaaaat
      721 ttcgcatagt attgtttttt tatgataaca caaaggaaag ggattgagaa tgaatggaag
      781 gccttttttc gattaataaa ggcaaaaaaa agacgaagta gctctaaagg cgaatcctgt
      841 tcaatggagg aaaaggtatt tccgttgaaa tccaaaaatc ttgccggatg ttatgtggtt
      901 tgatatcgaa ggcccttgtt atcttattga agccaccagg ctatgcagtt agccgttgat
      961 caggtaatca atattgcgac gatgactatt ggttgctgta tcagcctgtc atgaaatata
     1021 atttgcctga aatccttagt ggcatatcca gcggtagttg attcagaata gaggaagcat
     1081 tttgaatcag taattaatca tgccgttatt atgagccatc caaagaaata tgcatgaatt
     1141 caggatgaat gggaatgtaa atggttaaaa tttgtgatga atataaagtt ataacttcag
     1201 tatcttatct ctacctaaga tgccctttgt aaatggatcc ttgtcctcgt atagcttgtg
     1261 ttcagttaag gtttcggttt tatagccgac caaattattc tataaatatt gatttgttct
     1321 tctttctaaa tgttgaaacc cgggttaaat atttcttcta ataattaatt gcatggtatc
     1381 gttgttttta ccttcgtttg atttttactg gatgtaaaaa cgaatatcct gccaggcaag
     1441 aaataaagcc tttttctttg caattcagaa atatttcgaa tcacttactc tgttgagtat
     1501 caaggcgatc aacgccccgc caaaaaagcc gattactaaa acctgtgcca attctatatt
     1561 cgtaatattg agcgtcggca tcctgaaaag aaaattcagg actgacaaaa caaacaggat
     1621 cgtcacaata atgatggcta ttaatttcat ccggaatcaa tctttaaata tgaaaaatac
     1681 agctaaaatc agaaaggtaa aaccaattat atgattaagc cggaatactt cattcttgaa
     1741 taaaaaaaca gtaaaaacag taaaaataat caatgtgatg acttcctgca ttaccttcag
     1801 ttgccaaaga ttgaagggtc cgccattttc cacaaagcca atcttattgg ccggtatcat
     1861 gaagcaatat tcgaaaaatg ctatgagcca gctaatgatg atgactccag gcagcccgaa
     1921 cttgttgagc caggcaatat ccttgaattt aagatgcccg taccaggcaa atgtcatcag
     1981 gatattggac agaacaagca atacaaatgt aatgatccct ttattcattt ggttttcgtc
     2041 tgaatgtaat tatattgttt cggtcatctt taccattgtt gcatgatttc tggttgtggc
     2101 cttaacttta agtatttttt cgatatattg attcgtcagc ttcgttttgc tgtacttatc
     2161 ccttaccaga agataaacta ccttgttgcc taacacaatc ttatcaccgc aggtatccat
     2221 ttccatcagt gcttcagcgc ggactctatc aggaggttct gcaagcaaag tgtaatagag
     2281 tctttttttg tccgtttctt catgggtaaa tggattattg tcaatgattc ttcgcagggt
     2341 gtcttgtggt aatgccaatg ttgggacatc gaagcctaat cgttcccgga tggctttacg
     2401 tatgatggac tccactgatt cagcctcaga gagctcggat tttacccata tatttccact
     2461 ttggatatag gtttttggtt catgcaaacc tatgtcaatg agtattttcc tcaagtcttc
     2521 catcttaatc aggttttgac cattgacatt gatcccgcgc agaaatacaa tataatctat
     2581 catttatttc cgggtatatt gattcatcgt ttctgtgatg cctaaggtgc cgaacgcaag
     2641 tacagctttt gcggcatgtg cgatgatttc cggcaagtct tgctcctcct ctttattcca
     2701 tttcccaagt acaaaattta cttgtcttcc tcttttaaaa tccccgccta tcccgattcg
     2761 gattctggcg tagttttgat tacccaatac ttcattgatg ttttttaggc cattatggcc
     2821 tccatctgcg ccattgggcc tgatgcggat gcttttaaat ggaatattca ggtcatcgag
     2881 tatgacaatg accctgtctt gtggaatgga caatttgcgc atccagtatt gtagtgcctt
     2941 cccgctcaga ttcatgtagg tcgaaggttt caggatatag aaggtcctgc ccttgtattt
     3001 tatggaagac aagtctccaa gatgctcgtg tttgaatttt tctcctgcgt cactgactaa
     3061 tttatctgcg acatcaaacc cgatattatg tctggtgcca tcgtattcgg cacccatatt
     3121 gcccaagccg gcgactaaat atttcatctc gtcgttgttg acctcccgtt ttttaaaaaa
     3181 attgaaaatt cccatttgat gcgacaaaaa taggttttag tgcattaaat gtaaaaggtg
     3241 gattaaaggt tttttgctat ttacaaatat cttgttttat gtttgcggtg attttcgtgt
     3301 atttgatggg gcatttttca aatatacatg aggatttttt ttctcttgga tttgttaaac
     3361 attcattgaa tatgcaactg taacagcctc acccgggtga tgtacagtcc tgttttatct
     3421 aaatgacaca atgaatatgg gtcaaaaatt attgattgtc gagtcgcctg ccaaggctaa
     3481 aacgattgaa aagtatctgg gaaaggattt cgtagtcaag tcaagtttcg gccatgtgcg
     3541 cgatctggag aaaggtaata atgccatcga tgtggacaac aatttcacgc cacattatgt
     3601 cattactcct gataaggtca aagtcgtaaa ggacttgaaa gatatcagca aaaagtcgga
     3661 ggaggtgtgg ctggcaacgg atgaagaccg cgaaggagaa gccattagct ggcatctgtg
     3721 tgaagtgttg gggctcaacc cccataccac caagcgaatt gttttccgcg aaattaccaa
     3781 gcccgccatt cagaatgcag tcgccaaccc acgcaacctc gatctggatc tggtcaatgc
     3841 acaacaagcg cgccgggtac tggacagact cgttgggttt gagctttcag aggttctttg
     3901 gaggaagatc aaggcctcac tatctgcagg ccgtgtccaa tcggttgccg tgcgtcttgt
     3961 ggtggatcgt gaacgggaga tcaacaactt cgaggtagag actttcttcc gagtcaaggc
     4021 atccttcccg gt
//
LOCUS       DAOQMC010000216         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_11258_length_3999_cov_3.773073, whole genome shotgun sequence.
ACCESSION   DAOQMC010000216 DAOQMC010000000
VERSION     DAOQMC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11258_length_3999_cov_3.773073"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..1674)
                     /gene="radA"
                     /locus_tag="PKV31_08320"
     CDS             complement(304..1674)
                     /gene="radA"
                     /locus_tag="PKV31_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNB92903.1"
                     /translation="MAKAKTVYICSSCGANSPKWIGKCNSCGEWNTYQEEIIQSSNTN
                     RNYSSRTGESGPKVSDKPKLLKEVKSNSHERIPTGDDELDRVLGGGIVPGSVILIGGH
                     PGIGKSTLILQSAMSMKTTVLYVSGEESEEQIKMRADRIGNISGQCYIFGETNIDTIL
                     RQADALQPSAIVLDSIQTMFLEHLESSAGSISQIRETAAELQRYAKERNIAVIIIGHI
                     TKDGDLAGPKLLEHIVDTVLYFEGDRHYAYRLLRSRKNRFGSTDEIGIYEMRESGLTP
                     VINPSDVLSGHGGQSYSGNALGIIGEGSRTFMLEVQALVTRAVYGTPQRSATGYDTRR
                     MNMLLAVLEKKCQLPLGFNDVFLNVAGGIKVDDPSIDLALAAAIISSLQNVAVPDKFC
                     FVGEVGLSGEVRPVQKIDQKLLEAERMGIDTIFISKYSKYNVPANFRLNIQPIVKVEE
                     LLDLFS"
     gene            1903..2715
                     /locus_tag="PKV31_08325"
     CDS             1903..2715
                     /locus_tag="PKV31_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNB92904.1"
                     /translation="MANVVSERFVLAIKKLIKDGNIKSMRRISLELDYLPQSLSEVTK
                     GKRDVPVEVLQRFITRFNVNPIFLFMGEGEIFLDQDQQYSFKTLTIVTDSDNEERILH
                     VPVPAQAGYAEELNAPEMIRELPTYTLPDITYNSGTYRSFDVRGDSMEPTIDENDILV
                     CSYLEPSLWETGIRDHHVYVIVTRGDVVVKRVINNLRKHKHLELYSDNEEYKMYRVYG
                     NDIREVWYVNTKISKFRHNQSTPAQAYTQGEFSALRDTIKAQAELIKTLTKH"
     gene            complement(2744..3388)
                     /locus_tag="PKV31_08330"
     CDS             complement(2744..3388)
                     /locus_tag="PKV31_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB92905.1"
                     /translation="MNERRKAKFEEVASKSQLSLTVILENVHDVLNIGAILRSCDSVG
                     IRNIFVINNSGLKQHKSLTLGKRSSMGTRKWVDVRYFHDLDTCIEEVRKDYDVIAGAA
                     ICEKTINYLDFDYTGKVAILMGNEKEGLSKAALDHCDVLIRIPQVGMAESLNVSTATA
                     VILYEAFRQRLVAGKYDHLPDISEGQANELLNRLHEKVFDKTKSTKKYKIKAEE"
     gene            complement(3381..>3999)
                     /locus_tag="PKV31_08335"
     CDS             complement(3381..>3999)
                     /locus_tag="PKV31_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB92906.1"
                     /translation="KARLNNNGQTCIAAKRFYIHKNIYPKWKESFVEIIKSKKIGDPK
                     DHSNELSGLARNDLAETLQAQVDKTVSMGARKLLSGGVDKDNPSIYHPEIIEHIDSKS
                     PLSCEETFGPVAALFPFEDEQEMLSRVNASRYGLGASVWSTDTDHAIAIADQVNAGSV
                     AVNQMMSSDPRLPFGGIKKSGFGREMSMEGFLEFLNLKTIIVSNE"
BASE COUNT         1140 a          847 c          770 g         1242 t
ORIGIN      
        1 ccatgaattc tctcctttta caatattcca ctgccgcata tttcgcggtt gtcccttact
       61 tgtttcttta ctcatattaa cataacatat tcacgttcaa taaaatctat tcattcaaat
      121 aaaattgaaa gcctattgaa ttcatttcaa atttgacctt tgattagaca aagacagtcc
      181 ttttagggca aaaaaccgat aaaaatacaa aaattcgaat tattatatca atttttaacc
      241 gacttcaata aaagcaatta tgccataaat gagcatttgc ctcaacagca tacaaagctt
      301 ctattagctg aaaaggtcta gcaattcttc gacttttaca ataggttgaa tattaagccg
      361 aaagttggca ggaacattat actttgaata tttcgaaata aaaatggtat caatacccat
      421 cctttcagct tccagcaatt tctgatcaat tttttgaaca ggtcttactt caccactcag
      481 ccccacctcg cccacgaagc agaatttatc cggtacagca acattttgca gggaagatat
      541 gattgctgct gccaaagcaa gatcaattga aggatcgtcc acttttattc cgccggcaac
      601 attgagaaag acatcattaa agcctagtgg taactggcat tttttttcaa gtactgccaa
      661 cagcatattc atcctcctgg tgtcgtatcc ggttgcggag cgctggggcg taccgtaaac
      721 tgcccgagta acaagtgcct gaacttcaag catgaaagtg cgtgacccct cacctattat
      781 tcccagggca tttccactat acgattggcc cccatgacct gacaacacat cgcttggatt
      841 aatcacaggt gtaagaccgg attctcgcat ctcatagata ccgatttcat ctgtagatcc
      901 aaatcggttt ttccttgaac gcagtaatct gtaggcataa tgtcgatctc cctcaaaata
      961 tagtacggta tccacaatgt gctcaagtaa tttgggtccg gctaggtctc catccttagt
     1021 tatatgccct atgatgatga cggcgatatt tctttccttg gcatatcgtt gaagttcagc
     1081 agcagtttct ctgatttgac taatgctacc ggcagagctc tccaagtgtt ccaaaaacat
     1141 ggtttgtatg ctgtctagaa ctattgcaga aggttgtagt gcatctgctt gtcttaaaat
     1201 tgtatctata ttggtctctc caaagatgta acattgtcct gagatatttc cgattctatc
     1261 tgctctcatc ttgatttgct cttcgctttc ctcgccggag acataaagga cggtagtctt
     1321 catggacatt gctgattgaa ggatgagcgt agatttgccg atgcccggat gaccacctat
     1381 gaggataacg ctaccgggta ctattccgcc tcccaagaca cgatctaatt cgtcatcgcc
     1441 cgtaggtatc ctttcgtgtg aatttgattt tacttctttc agtaattttg gtttatcgga
     1501 aacttttggt ccggattctc cggtacgtga tgaataattc cgattagtat ttgatgactg
     1561 tatgatttct tcctgatagg tattccattc tccacatgaa ttgcattttc ctatccattt
     1621 aggggaatta gcaccacagc tggaacatat atatacagtt tttgctttag ccaaggtaaa
     1681 ttaaataatt ttgcctgcaa aggtatgatt aaaaggcgat aaagccaagt atccattgat
     1741 caacaaagcc aatataaaca taatcgggac aagaaaggga ggataatatc attcactatt
     1801 ttgcgtataa ttttatgatg tgtaaatatt atgataatat atgtgtgaaa aatatataat
     1861 attttaatac ctttgaacct gattaatacg aattaataaa atatggcaaa tgttgtgtcg
     1921 gagcgttttg tgctagcaat taagaaactg attaaagatg gtaatatcaa aagcatgcgg
     1981 aggattagcc ttgagctgga ttaccttcca caaagtctca gcgaagtaac caaaggcaag
     2041 agggatgttc ctgttgaagt gttgcaacga tttataaccc ggttcaatgt caatcctata
     2101 tttctcttta tgggtgaggg agaaatcttt ctggatcagg atcagcaata ttcattcaag
     2161 actctcacaa ttgttacgga ttcggacaat gaggaaagaa tcctccatgt tccggtaccc
     2221 gcacaagcag gttatgccga agaattgaat gctccggaga tgatccggga actacctacc
     2281 tatacattgc ctgatatcac ctataattca ggcacctacc gtagttttga tgttaggggg
     2341 gattcgatgg agcccaccat cgatgagaac gatatcctgg tctgtagtta tctggaaccc
     2401 tctctttggg aaaccggcat ccgggaccat catgtctacg taatcgtcac cagaggggat
     2461 gttgtagtaa agcgagtaat aaataatctt agaaagcaca aacaccttga gttgtactca
     2521 gacaatgagg aatacaaaat gtacagggtc tatggaaatg atatccggga ggtctggtat
     2581 gtcaacacaa agatttcaaa gttcagacac aatcagtcta caccggctca agcatatact
     2641 cagggtgaat tcagtgcttt gagggatact attaaagctc aggctgaatt aatcaagact
     2701 ttaacgaagc attgatgact gattcttcgg atgcatgatt tcactattct tcagccttga
     2761 ttttgtattt tttcgtactc ttggttttgt caaaaacttt ttcatggagt ctgttaagca
     2821 attcgttagc ttgaccttct gaaatatccg gtagatggtc atacttaccc gctaccaatc
     2881 gctgtctgaa tgcttcatat aatatgacag ccgttgctgt ggatacattt aaactttcag
     2941 ccatccctac ctgagggatt cggatcagga catcacaatg gtctagagca gctttagaca
     3001 gaccttcctt ctcatttccc attaaaattg cgaccttgcc cgtataatca aaatcaagat
     3061 agtttattgt tttctcgcag attgccgcac cggcaatgac atcgtaatct ttcctgactt
     3121 cttcaatgca ggtatctaaa tcatggaagt atctgacatc gacccatttt ctggtaccca
     3181 tggaacttct ttttcctaaa gtcagtgatt tatgctgttt taagcctgaa ttgttgatta
     3241 caaaaatatt acggatacct actgaatcac aggatctaag aatggcgcct atattaagta
     3301 catcgtggac attttccaga ataacggtga gtgaaagctg agattttgat gcgacttcct
     3361 caaattttgc ctttcgtctt tcattcatta ctgacaatga ttgttttcag gttgagaaat
     3421 tcgagaaaac cctccatact catttctctt ccgaatccgg attttttaat acctccaaaa
     3481 ggcaatctgg ggtcactgct catcatttgg ttcacagcaa cagaacccgc attcacctga
     3541 tcagcaatgg ctatggcgtg atctgtatcg gttgaccata cacttgctcc caggccatac
     3601 cggcttgcat taacccgact aagcatttcc tgctcatcct caaagggaaa taaagcggca
     3661 acaggaccaa aggtttcttc acaagataga gggcttttgc tgtcgatatg ctcaattatt
     3721 tccggatggt agatagatgg attgtcttta tcgacaccgc cggagagtaa ttttcttgca
     3781 cccattgaaa cagtcttatc gacctgggcc tgcaaggtct ccgcaagatc atttctggcc
     3841 aggccgctca attcattgga atgatccttc gggtcgccaa tcttcttgct tttaataatc
     3901 tcgacaaaag attctttcca ctttggatag atatttttgt ggatatagaa tctcttagca
     3961 gctatgcagg tttgaccatt gttgtttaag cgtgctttg
//
LOCUS       DAOQMC010000217         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_11299_length_3991_cov_4.332063, whole genome shotgun sequence.
ACCESSION   DAOQMC010000217 DAOQMC010000000
VERSION     DAOQMC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11299_length_3991_cov_4.332063"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PKV31_08340"
     CDS             complement(<1..813)
                     /locus_tag="PKV31_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HNB92907.1"
                     /translation="MKKLAFLFFICFSSLVSAQKSADTAQVKIPGRVNSATTLTQPYV
                     ILISSDGFRYDYLKKYNTQYLLDFAENGVWAKKGMYPTYPSITFPNHYSIATGLYPAH
                     HGIVDNKFYDPKRNEMYALGSKSILDGSWYGGEPFWSLAEKQGMMAASLFWVGSESDA
                     GGMRPSYYYKYHEKFTDDDKVRIIKGWLTLPEEVRPHFITLYFPEVDHMGHKYGPDAP
                     QTEESVHYVDTAINKLVKALQPLNLPINYIFVSDHGMIGVEKKDLVPMPAIDP"
     gene            1375..3744
                     /gene="pbpC"
                     /locus_tag="PKV31_08345"
     CDS             1375..3744
                     /gene="pbpC"
                     /locus_tag="PKV31_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNB92908.1"
                     /translation="MKWRSQKLRVLIVCAVLLAAFYFILPRKLFNPSYSIILEDRNGI
                     LMGARIAADGQWRFPPEGGDDTLSQKYIDAVLMYEDRNFYRHGGVNIRSLARATWQNI
                     KARKIVSGASTVSMQVISLYRGHKARNILDKLWEIILAMRLEVRYSKEEILKLYASHA
                     PFGGNTVGYGAATWRYFGKHNKDLTWAEAALLAVLPNDPALIHPGKNRDRLRLKRDNL
                     LRKLASKEVFDPANLDLYLEEELPDRPYSMPDVAPHLLSSMQRLTGKSYMHSSVDMLL
                     QQRLNDLLANYGLALKANEVNNAAICVADVMTGEVKAYVGNMPGTGSSHGEQVDIIQS
                     SRSTGSLLKPFLAMMAIGEGKLTTRTLLADIPVNIDGFRPDNFTYDFEGAVPLDVALR
                     KSLNIPFVLLLKEYNISRFLQNLRLLGVSRLDKSADHYGLSLIIGGGESSLWELCGTY
                     ASCARILNHYNENSSRYSRHDVRPLSMEINKIEYQVKDKSPSVLDAGSIFKIFDVLSE
                     NTRPGEYEAWKTVSGPGRIAWKTGTSIGFRDAWSIGVNSRYVVGVWIGNADGEGRPGV
                     IGLETAAPLLFKVFDLLPSAPWFAKPFDGLKKVIICRNSGLAPNDTCPKDTMEVPKHA
                     DMLALCTYDRSIALDKTGSFRVSSECYSPFQMVFKNYFLLPPAQQYYYSSKHPSYNSP
                     PMWFPGCDHGIESRQSVMQFIYPNNIRKLKLPRDANGKLNPVTFVVAHKDHSVALFWH
                     LDDRYMGTTKGKHSFSTVLPSGFHTLTVVDEAGISISTSFEAVIVSNND"
BASE COUNT         1126 a          737 c          911 g         1217 t
ORIGIN      
        1 aggatcgatg gcaggcattg ggactaaatc ctttttttcg acacctatca ttccatggtc
       61 ggagacgaaa atatagttga ttggcagatt caatggttgt aaagctttta ccaatttatt
      121 gattgccgta tcgacgtagt ggacgctttc ctcggtctgt ggagcatcag gcccgtattt
      181 gtgacccatg tgatccacct ctggaaaata aagagtgata aaatgaggcc tcacctcttc
      241 aggcagtgtc agccatcctt tgataattct cactttatca tcgtccgtaa atttttcatg
      301 gtatttgtag taatagctgg gcctcatgcc tcccgcatca ctctcggaac caacccaaaa
      361 caagcttgca gccatcatgc cttgtttttc tgccagagac caaaaaggct ctccgccata
      421 ccagcttcca tccaagatgg actttgagcc cagcgcgtac atttcatttc ttttcggatc
      481 gtaaaatttg ttgtccacaa ttccatgatg agcaggataa aggccggtag ctatggagta
      541 atgattggga aaggtaatgg aaggataggt aggatacata cctttcttag cccagacacc
      601 attttcggca aagtccagca agtattgggt attgtatttt tttaaataat catatcggaa
      661 tccatcagac gaaatcagga ttacataggg ttgggtcaat gttgtggcag aattgactct
      721 cccgggaatt ttcacctgtg ctgtatctgc ggatttttga gccgatacca gtgatgaaaa
      781 acaaataaaa aataaaaagg ccagtttttt cacggctgtt gttttttcaa aggtaaaaca
      841 acttccaaga ttttcaaaca aatggaaaga actgtgttga atacaaattg caaatgttta
      901 ccggataaaa gaatggtcga gtagctatga ataatgatga tacccttaat ttaatactaa
      961 acagaaagtc aggtgttgtt ttatttggca ttattttttc ttgataaaag ataaaccaaa
     1021 aatcaaagtg aataaaaatc ttatcgatgt tattataatg gacggggttt aaaacttgtg
     1081 tccaggttaa taataagtcc attgccttcc ggggtaaatt ccttaatcat tccccggcta
     1141 aatatcacct catttttgtc acaatcatta attattttga ctaatctccc ttggcttatt
     1201 attcgatttt attatgatat aaggccggtc tgtattagga tttttttcat actaacactt
     1261 gaattaattc tgcaattata aatatacaag aaaggacttt gttattgaac tgcaatagcc
     1321 ataattacaa aaactatccg gtatggatta aaattgattt aaatttgttt tcggatgaaa
     1381 tggcgttcac aaaaattgcg ggttctcatc gtatgtgctg tcttgttggc ggcattttat
     1441 tttattttgc ctcgcaagct gttcaaccct tcttacagca taattcttga agaccgaaat
     1501 ggtattctga tgggagcgag aatagccgct gacgggcaat ggagatttcc tcctgagggc
     1561 ggtgatgata ctttatcaca aaagtatatt gacgcggtgc tgatgtacga ggataggaat
     1621 ttttatcgtc atggaggggt taatattcgt tcattagcac gagcaacgtg gcagaatatc
     1681 aaggcccgca agattgtttc aggggcatca accgtttcca tgcaggttat cagcctttat
     1741 cgtggtcata aagcacgaaa tatacttgat aagctgtggg aaattattct tgcaatgagg
     1801 ttggaggtcc ggtattcgaa ggaggaaata ttaaagttgt atgcatctca tgctcccttt
     1861 ggaggcaata cagtgggcta tggggctgcc acctggcgat actttgggaa gcacaataag
     1921 gatttgacgt gggcagaggc tgcattattg gcggtgttac ccaatgatcc ggcgcttatt
     1981 catccgggga agaaccgcga cagattacgt ctgaagcgag ataacctact taggaaatta
     2041 gcatcaaaag aggtatttga tccagcaaat ctggatcttt atcttgaaga agagcttcct
     2101 gatcgtccgt attccatgcc tgatgttgct ccacatttgt tgtcttccat gcaaaggttg
     2161 accggcaaaa gttacatgca ttcatcggtt gatatgttgc ttcagcaaag acttaatgat
     2221 cttttggcaa attacggcct tgctctgaag gccaatgaag tcaataatgc cgcaatctgt
     2281 gtagcggatg taatgaccgg tgaagtcaaa gcttatgtcg ggaatatgcc aggaacggga
     2341 agcagccatg gtgagcaggt ggatatcatt cagtcaagta gaagtacggg aagtttatta
     2401 aagccatttc tggctatgat ggctatcggg gaaggcaagt tgacgaccag gacgttgttg
     2461 gcagatattc ctgtcaatat agatggattc cgtccggaca attttactta tgattttgaa
     2521 ggggcagtac cattggatgt agcattgaga aagtcactga atattccatt cgtattgctt
     2581 ctaaaagaat acaatattag tcgattttta caaaatctga ggttgctggg agtaagccgc
     2641 ttagataaat ctgctgatca ctatggtttg tcattgataa tagggggagg ggagtcgtca
     2701 ctttgggagt tgtgcggaac ttatgccagt tgtgcacgta tccttaatca ctacaatgaa
     2761 aattcaagcc ggtatagtag gcatgatgtc cgaccactct cgatggaaat aaataaaata
     2821 gaatatcagg tgaaggataa aagcccgagt gtgctggatg cggggtcaat atttaagata
     2881 tttgacgtcc taagcgagaa taccaggccg ggagagtatg aagcatggaa gacggtttcc
     2941 gggccaggtc gtattgcatg gaagactgga acctctattg gtttcagaga cgcatggagt
     3001 ataggagtta atagcagata tgtagttggg gtatggattg gaaatgccga tggtgaaggt
     3061 cgtccgggtg ttattggcct tgaaactgct gctccactat tgtttaaggt atttgactta
     3121 ttgcccagtg cgccttggtt tgccaagcct tttgatggtt tgaaaaaagt aattatttgt
     3181 agaaacagcg gattagcgcc taatgatact tgcccaaaag ataccatgga ggtaccgaag
     3241 catgctgata tgcttgcctt gtgcacgtat gaccgttcta tcgctttgga caaaaccgga
     3301 tctttccgtg ttagctcaga atgttacagc ccttttcaaa tggtttttaa aaactatttt
     3361 ctactacccc ctgcccaaca atattattat tcttcgaagc atccatcata caatagtcct
     3421 ccaatgtggt tccctggttg cgatcatgga attgaatcgc ggcaaagtgt tatgcaattc
     3481 atatatccga ataatatcag gaagttaaaa ttacctcggg atgcgaatgg caaacttaat
     3541 cctgttacct ttgtggtggc ccataaggat catagtgtcg cattattttg gcatttggat
     3601 gatcgataca tgggtacaac caaagggaaa cattcgtttt caactgtttt gccatcaggt
     3661 tttcatacct tgacggttgt tgacgaggcg gggatttcaa tttccaccag ttttgaagca
     3721 gttatagtct cgaacaatga ttgaaaaaga gttgagtatt tctgaccatc tatgcaaatc
     3781 tgacaaagat ttcaacgagt ttttaaatat gatttgtatt tggcagtagg tcttagttgc
     3841 cctagttggt attgaatgcg catcctgttc atattcagat tattaacccg tagttaacat
     3901 cttacaggtc atcggcctat gcctgaccat tctggagatg atgtcatcca ggatttcgcc
     3961 tttcatgcgg tgcctgttgt agcggtagca g
//
LOCUS       DAOQMC010000218         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_11484_length_3956_cov_4.918995, whole genome shotgun sequence.
ACCESSION   DAOQMC010000218 DAOQMC010000000
VERSION     DAOQMC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11484_length_3956_cov_4.918995"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKV31_08350"
     CDS             complement(<1..449)
                     /locus_tag="PKV31_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92909.1"
                     /translation="MKNLKLFIVIILGLFIVGCTQDDVVVDKKLPLVSDFDYKVINDW
                     NTTFLRIERYADGYRPGPAPRGLAYLGLAAYEACIPGMPEYKSFSGYFPGFQVPAGDP
                     NAEYNWPLVVNETYHYLIPLFFSQATQQQLNAADDMYNSNLNRLKTNV"
     gene            756..2366
                     /locus_tag="PKV31_08355"
     CDS             756..2366
                     /locus_tag="PKV31_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB92910.1"
                     /translation="MITVQNLALQYGKRVLFEDVNLKFTQGNCYGVIGANGAGKSTFL
                     KILSGEIAANRGLVQMEPGKRMAVLRQNHQEFDNYTAMDTVIMGHRKMYDIMIEKNNI
                     YMDPDATEADSLRAADLEAEFGEMGGWTAESDAGELLSNMGIPTALHTALMSELRGPD
                     KVKVLLAQALFGNPDYLLLDEPTNDLDALTVTWLSDFLADFRNIVIVVSHDRYFLDMI
                     CTHMVDIDFGAINIFSGNYTFWYETSQIMRRQTDINNKKVENKRQELMEFIARFSANA
                     SKSRQATSRKKALEKLNLEEIKPSSRRYPYIAFIPERQPGDILLKVDGLTKKDINGNV
                     LFDNISFTVNSDEKVGIICDRAVAMTAFFDVLAGKETPDAGTIEWGQTITYDYLPNDN
                     SAFFTGANDLTLVDWLRQYSEEKDEQFIRGFLGRMLFSGEEVFKKVSVLSGGEKVRCM
                     LSKIMLAHPNFLLFDDPTNHLDLESIITLNDAMKNFKGNIIFNSHDHQLMQTVSNRII
                     EILPMGTKESLSSYEEYLQQKKLQLAGA"
     gene            complement(2374..3324)
                     /locus_tag="PKV31_08360"
     CDS             complement(2374..3324)
                     /locus_tag="PKV31_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB92911.1"
                     /translation="MNKSLSAHLALLGLNIIYAANHLLAKGVTPYYLGPNGFVFIRVG
                     MTSLLFLLLYIALLREKIERKDFLRLFISGILGSGLSQLLFFNGLARTSAINTGVLMT
                     SIPIFTVILSFFFLKENITKYKISGIIIGAVGAIALTTLGKSPAFDSAIGDILIIANA
                     FVFAAYLVMIKPLMKKYHPVTVATFNFLSGLIFVALYPDLWTDLNNAHFEAYNQRVWM
                     ILIFVVFFATFITYLLNIFSLKYLSPSITGSYVYTQPALVILLAHIFTFIGWTDDVTG
                     SINSAKILFMTMIAIGVYLVSKPPTEGCSTTLAKKGKENN"
BASE COUNT         1242 a          688 c          893 g         1133 t
ORIGIN      
        1 acattggttt taagtctgtt cagattggag ttgtacatat catcagctgc attcaattgc
       61 tgctgagtgg cctgagagaa gaacaatgga attagataat gataagtttc attgacaacc
      121 aatggccaat tatattctgc atttggatca ccggccggaa cttgaaatcc cgggaaataa
      181 ccgctgaagg atttgtactc aggcatgccc ggaatacaag cttcataggc agcaagccca
      241 aggtaggcaa gacctctggg agcaggtcct ggacgataac cgtcagcgta tcgttcgatt
      301 ctaaggaaag tagtgttcca gtcattaatg accttgtagt cgaaatctga gaccaatggc
      361 agtttcttgt cgacaacaac atcatcttga gtacatccga ctatgaataa gccgaggatg
      421 attacaataa atagctttaa atttttcatt tttaaataaa tgtatgttat gataggttat
      481 ttaatataaa gaacaaatat acacctgtgc ccggccttat tttgaaaaat tcattaaaaa
      541 gttattaaaa tttgacattg tcattttata attagccctt ttattctctg tatttactaa
      601 agatttaagt atattatttg atggaaagag atgattgtgt tagttggtcg attccaggat
      661 taaatatatt gtaatctcac attctgtcaa tacctaatta cgcaatattc aggtatattt
      721 tttatacctt tgcggcttct taccaaaatc tcatcatgat tacagtccag aatttagccc
      781 tgcaatatgg caaaagagtc ctttttgaag atgtcaatct gaaattcacc caaggcaatt
      841 gttatggtgt cataggcgct aatggtgcgg gtaaatcaac ctttctgaag atcctgtccg
      901 gtgagattgc cgccaataga gggttagtcc aaatggagcc gggaaaacga atggcggttc
      961 ttcgtcagaa ccatcaggaa tttgacaatt atactgccat ggatacagtc attatgggac
     1021 atcggaagat gtatgacatc atgattgaga agaacaatat ttatatggat ccggatgcta
     1081 cggaagctga tagtctccgc gctgcagacc tggaggcaga atttggtgag atgggtggct
     1141 ggacggcgga aagtgatgca ggtgaattgc tgagcaatat gggtatacct accgctttgc
     1201 acactgcatt gatgtctgag ttgcggggac ctgacaaggt aaaggtattg cttgctcagg
     1261 ccctgtttgg caatccggat tatcttttgc tcgatgagcc taccaatgac ctggatgcat
     1321 tgacagtgac ctggctgtcg gacttcctgg cagatttcag aaatattgta atcgtagttt
     1381 cgcatgacag gtattttctg gatatgattt gtactcatat ggtggatatc gattttggtg
     1441 ccattaatat attttcagga aattatacct tctggtacga aaccagccag atcatgcggc
     1501 gccagactga tatcaacaat aaaaaggtcg agaataagcg tcaggaacta atggaattta
     1561 ttgcacgatt cagcgccaat gcctctaaat caagacaggc taccagtcgt aaaaaggcgc
     1621 tcgaaaagct gaatctggaa gaaatcaaac cttcgtcgag aagatacccc tatatagcct
     1681 ttattccaga acgacaaccg ggtgatattc ttctgaaggt ggacggtctg actaaaaagg
     1741 atattaatgg aaatgtcctg tttgataaca tcagttttac tgtgaatagc gatgaaaagg
     1801 tcggcatcat ttgtgaccgt gcagtagcga tgactgcatt ttttgatgtg ctggcaggaa
     1861 aggaaacgcc ggatgcaggc actatagagt ggggtcagac cataacctat gactatcttc
     1921 caaacgacaa ttcggcgttt ttcaccggag cgaatgatct cacccttgtt gattggctgc
     1981 gccagtattc ggaagagaag gatgagcagt ttattagggg ttttctgggc aggatgttgt
     2041 tttccggaga agaggtcttc aagaaggtca gtgtgttgag cggtggagag aaggttcgat
     2101 gtatgctctc caagatcatg cttgcacatc ccaattttct cctctttgac gaccctacta
     2161 atcatctgga tttggagtcc attatcacac tcaatgatgc gatgaaaaat ttcaaaggca
     2221 atatcatttt caatagtcat gaccatcagt tgatgcagac tgtctctaac aggatcattg
     2281 agatattgcc aatgggcacc aaggaaagcc tgtcctccta cgaggaatac cttcagcaga
     2341 agaaactgca acttgcagga gcctgaagta gcctcagtta ttttctttac cttttttggc
     2401 aagcgtggtc gaacatcctt ccgtaggggg tttggacaca agatacaccc ctatggcaat
     2461 catggtcatg aatagaattt ttgcgctatt gattgatcct gtgacgtcat ccgtccaacc
     2521 gataaaagtg aagatatgag ctaaaaggat gacaagtgca ggttgtgtat atacataact
     2581 cccggtaatg cttggggaca aatatttcaa agaaaaaata ttgagcagat aggtaatgaa
     2641 ggtcgcaaag aagacgacaa agattaaaat catccaaacc cgttggttgt aagcttcaaa
     2701 atgcgcatta tttaaatctg tccacaaatc tggatatagc gccacaaata tcagtccgga
     2761 aagaaaattg aaggtagcga cggtaaccgg atgatatttt ttcataagtg gtttgatcat
     2821 gaccagatag gccgcaaaaa caaatgcatt ggcaatgatc agtatgtctc ctatggcgga
     2881 atcaaatgcc ggtgactttc ccagtgttgt cagtgcaata gcccccacag cgccgatgat
     2941 aatgcctgag attttgtatt tcgttatgtt ttcctttagg aagaaaaaag aaaggatcac
     3001 cgtgaatatc ggtatcgatg tcatcaatac accggtattg atcgctgagg tacgtgcaag
     3061 accgttgaaa aatagcagtt ggctaaggcc gcttcccaat ataccggaga taaaaagtcg
     3121 aagaaaatct tttctttcga ttttttcacg taacaaggca atatataaaa ggagaaacaa
     3181 aagagaagtc atacctactc ggataaaaac aaagccattg ggacccagat aatacggagt
     3241 tactcctttg gcaagaaggt gattggcggc gtaaataata tttagaccca gcagggccaa
     3301 atgggcagac agggatttat tcacgcttac ttaatatccg gatagaaaat aatgtccatt
     3361 tcatttttac acaaaataag caagtcctgg tggttgtttt gctttggcga tgcgaaatca
     3421 gagaggagca tttcttaaac cgtgcttaaa aattatgagt aaaaaacatc agcgaaggta
     3481 gtgatttatt gcaagagtgt caaaaatcaa aactgatttg gtattgaagt gaggaaggaa
     3541 tagaagcctt atcatgataa aggacaattg agcatcaggt aatttgaaag ttatggtttt
     3601 tactatcaag agtgtttaaa tcaaaataaa ggctttgcat ctttaaggca atgaagtaca
     3661 aaattatatt tgggacgcat ttagcaaaat gtttatttgt atatatgtaa tattgtggaa
     3721 gatagaaaaa ccactaaaga tggggagtgt atgagagtat tgatcaacca tactatggta
     3781 taattttgaa aaaaaggagg tggcttcggc cgaatttttg aatatcatgg accgattttt
     3841 ttgatcttat aaaataatat tttgttgtaa gttattgata atgagtattt ggaatgggtg
     3901 gcaaaaatat ttttcaaaaa aaagggtaaa aatattttgt gggagagaaa aggtat
//
LOCUS       DAOQMC010000219         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_11581_length_3937_cov_4.889490, whole genome shotgun sequence.
ACCESSION   DAOQMC010000219 DAOQMC010000000
VERSION     DAOQMC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11581_length_3937_cov_4.889490"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1148)
                     /locus_tag="PKV31_08365"
     CDS             complement(153..1148)
                     /locus_tag="PKV31_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNB92912.1"
                     /translation="MNLRHPHNIFGEEKRIERVIGRYDEGVKGPLVICIGGIHGNEKA
                     GLKAIDLMLKMLEVEHITNTNFEFKGRFLGVSGHLAAIRQNQRYIEKDLNRLWLKDKI
                     EWLSQQNRNTLSAEDLEMFDLESLITHEIRDYGADRTVILDLHTTSADGGIYTIVPDH
                     PLAIEYALGIKAPVVLGFLEGLKGTTLHYFTSHNFPGEVTSICFEAGQHDDPMSVNRA
                     IAAITNCLRYAGCVEGRHIEGRHIEVLSEYSGQLPPLTRLAYRYPINPDEGFRMLPGF
                     QNFQPIHKGQHLADNHVGPILSPMDGFILMPLYQSKGEDGFFIVKRASLYKDDEF"
     gene            complement(1151..1810)
                     /locus_tag="PKV31_08370"
     CDS             complement(1151..1810)
                     /locus_tag="PKV31_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92913.1"
                     /translation="MKYLPEAIEFLYQQDAGWAVLVELTSIPEFGVPEPPYIELMSSI
                     VSQQLSTKVAKVIWNRFVDLFPRGFPEPSLVIELSDLQLREIGFSNAKANYVKNIAGH
                     FLAAPYTRDSLEAMDDLELLKEFSSIKGVGEWTVQMLQIFNLHRPDILPVKDLGVRQA
                     YSNFYGLNLTERELILHMNEKAEKWSPYRSAASLLLWNWKNHQFPGLEEIQKLNKNSK
                     N"
     gene            1873..3621
                     /locus_tag="PKV31_08375"
     CDS             1873..3621
                     /locus_tag="PKV31_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB92914.1"
                     /translation="MKFLRFLTSLLIIIFCHFNQLFAQVYIEPGVQRELKEKGFSSCV
                     LAFQPLILKDKIPANRSKTEKGNTAYNLLKNHLKDNILEACTILDKSKIKYRINYLSS
                     TINAYHCPEAIVIKLAGLTNVKAVLYNYSFDGIKGDFQENSGGNRDSYTWGIIKTEAN
                     VIQELGYRGQGAVVGGNDTGFDWTHPAITTRYRGYNNGGVDHNYNWHDAIHEVSPLSN
                     PNQNNPCGLSLTAPCDDHSHGTHTVGTMVGVKEDNEVIGMAPDAKWIGTRNMERGNGS
                     LQSYLEALDWFLAPTDTNDLNPDPAKSPHVINNSWYCSEEEGCNVDNWNLMQVSIANL
                     KAAGIVVVASAGNSGPRCESIAFVPGMLDEVFSVGATNQVDTIAGFSSRGLVTADGSN
                     RLKPDIAAPGVGVLSSVLNHQYSYSSGTSMAGPHVAGLVALMVGANPDLAGQVDTIQE
                     IIKRTADPLTLDQTCNGLSGLDIPNPIYGHGRINAFKAVTAAINHGTNKTVSNKYHSL
                     QLYPNPANDQLVIGGWLNDGVVHVEICEMNGKPVFSSILNQRYSTLNLEPVPPGTYLV
                     RAIADRSVYIGKFVKI"
     gene            3707..>3937
                     /locus_tag="PKV31_08380"
     CDS             3707..>3937
                     /locus_tag="PKV31_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB92915.1"
                     /translation="MSKLYAIVDIETTGGSAGMDRITEIAIVHHNGENVTGHYSTLIN
                     PERSISPFISGLTGITNEMVKDAPKFYEVAREI"
BASE COUNT         1211 a          851 c          772 g         1103 t
ORIGIN      
        1 ataaagccaa agataaatta cagcaatggt ttgattcaga agaaatacag aaagcaataa
       61 aggcaatcga taaaaaaatg gcaaagcaat aattgccatg aatatcaaaa agagtaataa
      121 tatttggggt ttaagttaat gaatcggcac tttcaaaatt catcgtcttt gtataatgaa
      181 gctcgtttta caataaaaaa tccatcctct cccttacttt ggtataatgg catcagaatg
      241 aacccatcca ttggactaag aatgggcccg acatggttat cagccagatg ctgaccttta
      301 tggattggtt gaaaattttg gaagcccgga agcattctaa atccttcatc cgggttaatt
      361 gggtaacggt aagccaacct ggttaagggc ggaagttggc cggaatattc agacaatact
      421 tctatatgac gaccttcaat gtgcctgcct tcaacgcacc ctgcataacg cagacagttg
      481 gtaattgcag caattgccct gttgactgac ataggatcat cgtgctgacc tgcctcaaaa
      541 caaatacttg ttacctctcc ggggaaatta tgacttgtaa agtagtgcaa agtggttcct
      601 ttcaatcctt ccagaaaacc caataccaca ggagctttaa tacccaatgc atactcgatg
      661 gcaagaggat ggtcaggtac tatcgtatag atgcccccgt cagctgaagt agtgtgcaga
      721 tcaaggataa ccgtcctgtc tgcaccataa tctcgtatct cgtgcgttat caatgattca
      781 aggtcaaaca tttccagatc ttccgccgac aaagtatttc tattttgctg acttaaccat
      841 tcaatcttat ccttcaacca taatctgttc aaatcctttt caatgtacct ttgattctgt
      901 ctgatcgcgg caagatgtcc ggacacacca agaaatcgac ccttaaattc aaaattagta
      961 ttagtgatat gttcgacttc aagcatcttc agcatcaaat caatggcttt taaaccagcc
     1021 ttttcatttc catgaatgcc cccgatacag attaccagtg gaccttttac accctcgtca
     1081 tagcgtccta tcacccgctc tatccttttt tcttcaccaa agatgttatg aggatgtctt
     1141 aaattcatgt tcaattttta gaatttttat tgagcttctg tatttcttcc aatccgggaa
     1201 attggtgatt cttccaattc cacaataata atgaggcagc acttctatat ggactccatt
     1261 tttccgcttt ttcattcata tgcaatatca attcacgctc cgtaagattc aatccataaa
     1321 aattactata agcttgcctt actcctaaat ccttgacagg caagatatcc ggacgatgta
     1381 ggttaaatat ctgcaacatc tgaactgtcc attcgcctac acccttgatg gaagaaaact
     1441 ccttcaacaa ttccagatcg tccatagcct ccaaagaatc ccttgtatac ggtgcagcaa
     1501 ggaagtgccc ggctatattt tttacataat ttgcctttgc atttgaaaat ccaatctcac
     1561 gcaattgcag atcactcaac tctataacaa gggaaggctc aggaaatccc cggggaaaaa
     1621 ggtcaacaaa acgattccat attactttcg ccactttggt actcaactgt tgtgaaacaa
     1681 tggacgacat caattcaatg tatggtggtt caggcacacc aaattcagga atggaggtca
     1741 attcaaccaa aacagcccat ccggcatctt gctgataaag aaattcaata gcctcgggta
     1801 aatatttcat ttgtcaaagc tatatattta ttttaaaatg tagctatctt tgaaaactaa
     1861 tcaatcaagt caatgaaatt tttacgtttc cttacctctt tgctcattat tatcttttgt
     1921 cactttaatc aactgtttgc tcaagtgtat atcgagccgg gtgtacaaag agaattgaag
     1981 gaaaaaggct tctcttcatg tgtcctggca ttccagccat tgattttaaa ggataagata
     2041 ccggccaatc gttcaaaaac ggaaaaagga aatacggcct acaatctttt aaaaaatcat
     2101 cttaaggata atatcctgga agcctgtaca atactggata aaagcaaaat aaagtaccgt
     2161 atcaactatc tatcttccac cattaatgcc tatcattgtc ctgaagctat tgttatcaag
     2221 cttgcaggat tgacaaatgt caaggctgta ctttataact attcatttga tggaataaaa
     2281 ggagattttc aggaaaattc tggcggcaac agagactctt atacatgggg gattattaag
     2341 acagaagcaa atgttataca agaactgggt taccggggcc agggcgcggt cgtcgggggt
     2401 aatgatacag gatttgactg gacacatcca gcgataacca ccagatacag gggttataac
     2461 aatggaggtg ttgatcataa ttacaactgg catgatgcta ttcatgaagt tagtcctttg
     2521 agtaatccca atcaaaacaa cccttgcgga ttgagtctta ccgctccctg tgacgaccac
     2581 agtcatggca cacataccgt aggcactatg gtgggtgtaa aggaagataa tgaagttatc
     2641 ggaatggctc cggatgctaa atggattggc accagaaata tggaacgcgg caacggtagc
     2701 ctccagtctt atcttgaagc tttggattgg tttttggctc caacagatac aaacgatctc
     2761 aatccggatc ctgccaaatc accacatgtc atcaacaaca gctggtattg ttctgaagaa
     2821 gaaggttgta atgtagacaa ctggaatctc atgcaagtct ccattgccaa tctaaaagct
     2881 gccggcatag tggtagtggc atcagcaggc aattcaggac cacgttgtga atctatcgca
     2941 tttgtcccgg gaatgctgga tgaagtgttt tcagtcggtg caaccaatca agtcgatacc
     3001 atagcaggct ttagtagtag gggtttagta actgcagatg gttcaaacag actcaaacca
     3061 gatatcgctg cacccggagt aggtgtatta tcctctgtcc tgaatcatca atatagctat
     3121 tcttcgggaa ccagcatggc aggtccacat gtagcaggcc ttgtcgcctt aatggtaggt
     3181 gcgaatccgg atctggcagg tcaggtagat accattcagg aaataattaa aaggaccgct
     3241 gatccactta ccctcgatca aacttgcaac ggactttccg gtctggatat tcctaatccg
     3301 atatatggtc atggaagaat caatgcattc aaagcagtca cagctgcaat caatcatggt
     3361 acaaacaaga cagtctctaa taaataccat tctttgcaat tgtatcccaa cccggccaat
     3421 gaccaactgg ttattggagg atggttgaat gatggtgtcg tgcatgtaga aatatgtgaa
     3481 atgaatggta agccggtctt ttcctccatt ttgaaccaga gatattcgac gctcaatctg
     3541 gagccggttc ctccgggtac ttatcttgtc agagcaattg ctgatcgttc tgtatatata
     3601 ggtaaattcg taaaaattta aaagtccccg gccttttgca ccagaaagtt ttatccggtc
     3661 atgtatatag agaactgaca ttgatcaaca aaatttacaa caatatttgt ccaagctata
     3721 cgccatagtc gatatcgaaa ctacaggagg ttcagcagga atggacagaa tcactgaaat
     3781 cgccattgtc catcacaatg gagagaacgt gaccggacac tactctaccc tgatcaatcc
     3841 ggaacgatcc attagtcctt tcatttccgg actgaccgga ataactaatg aaatggtcaa
     3901 ggacgcaccg aagttttacg aggtagccag agaaatc
//
LOCUS       DAOQMC010000220         3906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_11726_length_3906_cov_4.958193, whole genome shotgun sequence.
ACCESSION   DAOQMC010000220 DAOQMC010000000
VERSION     DAOQMC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11726_length_3906_cov_4.958193"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKV31_08385"
     CDS             complement(<1..478)
                     /locus_tag="PKV31_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92916.1"
                     /translation="MINNFKSLSLKAFQQRFTSTYQCIDYLVSIKWPGEFICKQCNHN
                     KYCKGKKYGDRQCTLCKKIESPKAGTLFHSMKIPLETAFYMLFLIVTDKKGCPSTHLA
                     RQTGLQQKTCWLFRRKVMAAMESDHQHLLEGEVDLVEIELNPSCAYADEILRRLEVS"
     gene            877..2379
                     /locus_tag="PKV31_08390"
     CDS             877..2379
                     /locus_tag="PKV31_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNB92917.1"
                     /translation="MTGNFHSLLSPYAILAIIGIYFLVLIIISGLTGKNTTNSKFFLA
                     GRSAPWILVSIGMIGASLSGVTFISVPGKVGVEGPNQYFSYLQFVFGNLAGYLFIGSV
                     LLPLYYKHNLTSIYGYLEKRLGFSSYKTGAAFFLISRGIGSALRLYLAAMVLHKFCTA
                     PLGLPFAATVFLTIGLIYVYTAKGGINTILYTDVLLTFSMLSALVMCVVIISKSMGNS
                     LTSMISQVYHSEYSKVFFFSDGWSDPNNFFKQFLSGALIAIVMTGLDQDMMQKNLSCR
                     TLRDSQKNMFIFCINLVIVVTLFLTLGAALYLFADQQGIPLPAKSDEFFPMMAMQYLG
                     VTVCVLFFVGLIASTYSSSDSALTALTTSICVDFLDIEKKDYSERQSKRIRTIVHLSV
                     SIALFFIILAFYYMANDAVIVMVFKIAGLTYGPLLGLFAFGIFTKYVPDNRWVPWICV
                     VCPLITWWIDNNSDWLLGGFQFGFLNLALNGLLIFIGLRIVTVGRVKHSL"
     gene            2476..3762
                     /locus_tag="PKV31_08395"
     CDS             2476..3762
                     /locus_tag="PKV31_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB92918.1"
                     /translation="MEQILKLSGEFIDCLDEMEQGSANLFITGKAGTGKSTLLDLFRR
                     STSKNIVVLAPTGIAALRIQGQTIHSFFKFPPRLILASDIQLKPGLLGLVRKLEILVL
                     DEISMVRADILDGINRMLQLHRKSNLPFGGVRLIVFGDIYQLPPIVNSIEEKQFFSTE
                     YGSPYFFSSHIWRSLENIHFISLSTIYRQTDRRFIRLLDDIRHGIVDYDTLEELNHRV
                     DEAFEEHGYIHLSPRNSTMQRINQRKLDELEGEERSYLSVITGEYNAQFAPTDPILKL
                     KVGAQVIFVRNDPERIFVNGTIGTITILDNNSITVRIVPDGKEVTVTRASWDMVRYKL
                     DENDNGKIKPEVVGSFEQFPLKLAWAMSIHKSQGQTFDRVILDMEGGAFEFGQTYVAL
                     SRCRTLEGIVLKRPLKPRDIMVDQRVVDFYFDHFRG"
BASE COUNT         1033 a          774 c          830 g         1269 t
ORIGIN      
        1 cggaaacttc caatctgcgc agaatttcat ccgcataagc gcaacttggg ttaagctcta
       61 tctcaaccaa atccacttcc ccttcaagta aatgttggtg atcactttcc atagctgcca
      121 tcaccttacg tcgaaatagc cagcaagttt tttgttgcaa acctgtttgc ctggccaaat
      181 gtgtggaagg gcagcctttt ttgtctgtaa caataaggaa gagcatgtaa aacgctgttt
      241 cgagaggtat tttcatagaa tgaaacaggg ttcccgcttt agggctttct attttcttgc
      301 aaagggtgca ttgacgatca ccatatttct tgcctttaca gtatttgtta tggttgcatt
      361 gtttgcagat aaactcaccc ggccacttga ttgaaacaag atagtcgatg cattggtacg
      421 tggaagtaaa tctttgttgg aatgccttca gggaaagaga tttgaagtta ttaatcatgt
      481 taaaagaatt gggtaatcag aaaaaaattt acgctgtgaa ggtaagaata aatttttgaa
      541 tgagcaaaag ggagggggtg tttttttgac aaaaatatta agtacgtatg aaaataatac
      601 tgcaatgaca ggtagatctt taaatatttg ccttcggcat tctaaaatga cagaataatg
      661 atcggatctg aaagaaattc tcttccaaga gtatgggaat gtgttcttta cagtattact
      721 taactactta gttaaagtta caccccaact atttgatttt taccagatat ttctttgtaa
      781 accaattcac tatcatcata acactccact tttgatcaat ttctgttgtc ctcttttcgt
      841 aaatattctt tttctttgtc attccaacct aattttatga caggcaactt tcattcttta
      901 ctttcaccct atgctattct tgcaatcatt gggatatact ttttagtcct catcattata
      961 tcaggattaa caggaaaaaa caccactaat agcaaatttt ttcttgcagg ccgtagcgct
     1021 ccctggatcc tggtgtcaat tggcatgata ggggcttcat tgtcaggagt gacatttatt
     1081 agcgtccccg gcaaggtagg tgtggaaggt cccaatcagt atttttccta tttgcaattt
     1141 gttttcggta atctggccgg ctaccttttc attggatccg tcttattgcc cttgtattac
     1201 aagcataatc tgacttcgat ttatggatat ttggaaaaac ggcttggctt ttcttcctat
     1261 aagaccggtg ccgctttctt tctgattagt agaggaatcg gttctgcctt gaggctttac
     1321 ctcgcagcca tggttttaca caaattttgt actgctccat tgggcctgcc cttcgccgca
     1381 accgtttttc ttactattgg tttgatttat gtctatacgg cgaagggcgg catcaacact
     1441 atcttataca ccgacgtgtt attgaccttc agcatgctga gcgccttggt gatgtgtgtg
     1501 gtaatcatca gcaaatccat gggcaacagc ttgacttcta tgatttctca ggtgtatcat
     1561 agtgaataca gcaaggtgtt tttcttcagt gacggatggt cagatcccaa taatttcttt
     1621 aagcagttcc taagcggtgc tttgattgca attgttatga caggtttaga tcaggacatg
     1681 atgcaaaaga atctttcctg ccgcaccctc agggattccc aaaaaaatat gtttatcttt
     1741 tgtatcaatc tggttatcgt agtgactctc tttctgacac tcggagcggc actctatcta
     1801 ttcgctgatc agcaagggat tccgttgcct gccaagtcgg atgaattctt ccccatgatg
     1861 gctatgcaat atcttggtgt taccgtgtgt gtccttttct ttgtcggtct gatagccagc
     1921 acttattcaa gctcggattc ggctctgacg gctctcacca cctccatttg tgtggacttc
     1981 ctggatattg aaaagaaaga ctattcagag agacaaagta aaagaatccg gactattgtt
     2041 cacctttcgg tatccatcgc cttattcttt atcatcctgg ccttttatta tatggctaat
     2101 gatgctgtca ttgtaatggt tttcaagatt gcaggcctga cttatggacc tctgttgggc
     2161 ttgtttgcct ttggaatttt tactaagtat gtaccggaca ataggtgggt gccatggatc
     2221 tgtgttgtgt gccctttgat tacatggtgg attgacaaca attccgattg gttactggga
     2281 ggatttcaat tcggctttct caatcttgct ctcaacggcc tcctgatatt tatcggattg
     2341 cggattgtta cggtgggccg cgtgaagcat agtttataac ctttattatt tattcgatac
     2401 acttggctgt atttaccatt tagccatact tttcctttcg ttttcatcta gaaatgctat
     2461 cttgcattga gcagtatgga acaaatactc aaactttccg gagaattcat tgattgcctt
     2521 gacgaaatgg aacaaggtag tgcaaatctt tttattacag ggaaggcggg taccggaaaa
     2581 tctaccctgc tcgatttgtt caggcgctct acttcaaaga atattgttgt acttgcacct
     2641 acaggcattg ccgcacttag gattcaaggt cagaccatac acagtttttt caagttccca
     2701 ccccggttga tcctggcgtc cgatattcag ctgaaacccg gcttgttggg attggtccgt
     2761 aagctggaga ttctggttct ggatgagatt tctatggtta gagccgacat ccttgatgga
     2821 attaatcgca tgcttcaact gcacaggaag agtaacttac cttttggcgg tgtcaggctt
     2881 atcgtttttg gagatatata tcaattacca ccaattgtaa acagtattga agaaaaacag
     2941 tttttctcca ctgaatacgg ttctccttat tttttttctt cccatatttg gagaagtctt
     3001 gagaatatac attttatcag cctttccaca atttatcggc agacagacag aaggtttatt
     3061 agattgctgg atgatatccg gcatggaatt gtggattatg atacattgga agagctcaac
     3121 catcgagtgg atgaggcttt tgaagaacat ggctatattc acctgagtcc tcgtaattct
     3181 acgatgcagc ggataaatca gagaaaatta gatgaattag agggagagga aagaagttat
     3241 ctttcagtga tcaccgggga gtacaacgct caatttgcgc ccactgaccc tattttgaaa
     3301 ttgaaagtag gggcccaggt catcttcgtt aggaatgatc cggaacggat ttttgtcaat
     3361 gggaccatag gcactataac tatcctggac aataactcta ttacagttag aatcgttccg
     3421 gatggcaagg aggtgaccgt aacacgcgcc agttgggata tggtccggta taaacttgac
     3481 gaaaacgaca atggtaaaat caagcctgaa gttgtaggca gctttgaaca gtttcccctc
     3541 aagctggcat gggcaatgtc tattcacaaa agccagggcc agactttcga cagggttatc
     3601 ctggatatgg aagggggagc ctttgagttt ggtcaaacct atgtggcttt aagtcgatgc
     3661 cgaacactcg aaggcattgt gctaaaacgc ccgcttaaac cgcgtgatat aatggtggac
     3721 caacgagtgg tggatttcta tttcgatcat ttcagaggat aaaaatagtc ttgtgaatgc
     3781 cgggtccatg attagggtaa atccatctta tcttctttag agcttttaat gaatagaaga
     3841 taggaaactg cgaaaatgtt ccatatgata tttccacttg tattaacttt atacctataa
     3901 aaattt
//
LOCUS       DAOQMC010000221         3885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_11868_length_3885_cov_4.436292, whole genome shotgun sequence.
ACCESSION   DAOQMC010000221 DAOQMC010000000
VERSION     DAOQMC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11868_length_3885_cov_4.436292"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKV31_08400"
     CDS             <1..524
                     /locus_tag="PKV31_08400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNB92919.1"
                     /translation="IRIGWYLMHYIKTLASEVEVNILDCRKYEVPLDDATYKNDGSAP
                     KHLQPLVELFFRTDGFILLSPEYNGSYTPSLKNLLNHFPKLDHKACGIVTASDGALGG
                     MRAAQQLQQLIAAHFGIISPYMLIVPHVGGKFNEEAELTDETFSRHIIRFVDEYLWLF
                     KALFDSRKKAFKN"
     gene            543..1757
                     /locus_tag="PKV31_08405"
     CDS             543..1757
                     /locus_tag="PKV31_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB92920.1"
                     /translation="MKLGILREGKIPHDDRVALTPAQCRILMGDPHNFSIVVQPSPTR
                     CFKDEEYIAAGIPVQEGLDDCNIILGVKEVPIKDLMQDKTYFFFSHTIKKQPHNRKLL
                     QAILEKRITMIDYETLEDSNGKRLIAFGKYAGIVGAHNGIWTYGKRTGLIDWPRLKDL
                     HDYEEAKDVYSSTSLPPFRIALTGSGRVAMGAISALDEMGIREVSPEEYLKNISYDHP
                     VYTHLSSQEYVRHKEGREFEKKDFYTNPQNYESAFEDYAEVTDIFLNCIFWNIHSPAF
                     FSLDDISNRKFRISVIADITCDIAPASSIPTTYRASTIDDPVYGIDRLTFAETAPYQE
                     NSIDIMAIDNLPNELPRDASKYFGSKFVKVIIPELEAEHSDIIQQATIAKDGKLMPRF
                     KYLEDYIATTNV"
     gene            complement(1785..3215)
                     /locus_tag="PKV31_08410"
     CDS             complement(1785..3215)
                     /locus_tag="PKV31_08410"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HNB92921.1"
                     /translation="MSVFRTGLLLSFILLANSVFAQSGYKIEIKINNYKNDTLLLGYY
                     LGDRQYVKDTAISKKGVFSFTGKNDLDPGVYMAVLLPEKNVFQFVVDKDNRFFSIEAD
                     GANLSKKLKVKNSDENTIFFNYIHFLTSQRPMADSLRSKQQIIQDPVEKQKLAEKIDE
                     IDQRVYKMQDDYVSKYPQSFTAKLIQTSKEQFIPKYGQNDDMETKLRQYNKYKDHYLD
                     NMDFSDERLFRSPVYFQKIDNYIQNITLQQPDSLIKALDIVLAKVKVIPDAFKFYLSH
                     YFNFFIKSKFVGMDAVYVHLIDQYYSKGLAPWVDKENLQKMVDAANDARPTLVGRIAP
                     NLTFINQKDSMDIRLHDIVSPYTVMFFWAPDCSHCEKAVPIVLDFYEKYKDKGVKVFA
                     VCTGLLDEAKDCWKNVVKKHMEPFVNVYDPVLLSNFKVIYNIKSTPQFFILDKDKKIL
                     SKGIGADQLNEVMGKIMEFDKNNHQQ"
     gene            complement(3292..>3885)
                     /locus_tag="PKV31_08415"
     CDS             complement(3292..>3885)
                     /locus_tag="PKV31_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNB92922.1"
                     /translation="PSPSLAISLANKIDASILNAGDGTHEHPTQALLDAFSMMEQIGD
                     LKGRNIAIVGDIIHSRVALSNIFCLKKLGAKVRVCGPPTLIPRYISELGVEVSYDLED
                     TLKWCDVANVLRIQLERQNTKYFPSLREYSRYFGINRQILEKVNRNIVIMHPGPINRG
                     VELTSDVADSPASIILQQVENGVAIRMAALFLLATRV"
BASE COUNT         1212 a          762 c          763 g         1148 t
ORIGIN      
        1 caatacgcat aggttggtat ttgatgcatt acatcaagac tttggcatct gaagttgagg
       61 tcaatatact tgattgtcgg aaatatgaag tcccactgga tgatgcaaca tacaaaaacg
      121 atggaagcgc tccaaagcat ttacaacctc ttgtagagtt atttttcagg acggacggat
      181 tcattttact ttcacctgaa tataatggga gctatactcc atcgctaaaa aatcttctca
      241 atcattttcc taagctggat cataaggcct gtggtatagt aacagcttct gatggtgctt
      301 tgggaggaat gcgtgcagct cagcagttgc agcagctcat tgcagctcat ttcggaatca
      361 tctctcccta tatgctcatc gtgcctcatg tcgggggaaa attcaatgaa gaagcggaat
      421 tgacagacga gactttttca aggcatatta tcagattcgt cgatgaatat ctttggcttt
      481 tcaaggcatt atttgattca aggaaaaagg catttaaaaa ttaacaggaa acgaaaaaat
      541 acatgaagtt aggaatactt agagaaggga aaataccgca tgatgacaga gtagcattga
      601 ctcctgccca gtgcaggata ttgatgggag atcctcataa tttcagcatt gtcgtacagc
      661 cctcacctac aagatgcttt aaagatgaag aatatattgc tgcaggaatc ccggttcagg
      721 aaggtctgga cgattgtaac atcatacttg gagtaaagga agttccaata aaggatttga
      781 tgcaggataa gacctatttt ttcttttcgc ataccatcaa aaagcaacct cataacagaa
      841 aattacttca ggcaattctt gaaaaaagaa ttaccatgat agattatgaa actcttgaag
      901 acagcaatgg aaaaaggctg atcgccttcg gaaaatatgc aggaatagta ggggctcaca
      961 atggaatatg gacttatgga aaaagaaccg gattgataga ttggccgcgc cttaaggatc
     1021 tacatgatta tgaagaagct aaggacgtct attcatcgac ctccctacct ccattcagaa
     1081 tagccttgac gggtagtggc agagtagcga tgggagctat ttcagccctg gacgaaatgg
     1141 gcatccgtga agtatcaccg gaagaatatc tgaaaaatat aagttacgat catccggtat
     1201 atacacatct atcctcacag gaatatgtca ggcataagga aggtcgtgag ttcgaaaaaa
     1261 aggattttta caccaatcct caaaactacg aatctgcttt tgaagattat gctgaagtga
     1321 ctgacatttt cctaaactgt attttttgga acattcattc accggctttc ttcagtctgg
     1381 atgacatttc gaacagaaaa tttcggattt cagttattgc ggacataact tgtgacatcg
     1441 ctccggcatc ttccattccc acaacatacc gggcaagtac catcgatgat cccgtgtatg
     1501 gcattgaccg tttgacattt gccgagacag cgccatatca ggaaaatagt atagatataa
     1561 tggcaatcga taatctaccc aatgaattgc cccgggatgc atccaagtat ttcggcagta
     1621 aatttgtgaa agtcataatt cctgagcttg aagctgagca ttctgacatc atacaacaag
     1681 caactatagc aaaagatggt aaactaatgc cccggtttaa atatctggag gattatatag
     1741 ccacgactaa tgtataggcc ggtataattg gaaagccgga aattctattg ttggtggtta
     1801 tttttatcaa actccataat tttacccata acctcattca gctggtcggc accaattcct
     1861 ttggaaagaa tctttttatc tttatcgagg atgaagaatt ggggtgttga tttgatatta
     1921 tagataacct taaaattgga aagcaacacc ggatcgtaga cattgacgaa aggctccatg
     1981 tgttttttca ccacattctt ccagcaatct ttcgcctcat ccaatagtcc ggtacatact
     2041 gcaaagactt ttacgccttt atccttatat ttttcataga aatccaggac aatgggaaca
     2101 gctttctcac agtgcgaaca atcgggtgcc cagaaaaaca tgactgtata aggagacact
     2161 atatcatgca atctgatgtc catactgtct ttttgattga taaaagtcag gttaggtgca
     2221 attctgccta ccagtgtcgg tctggcatca ttggccgcat ctaccatctt ttgcaagttt
     2281 tctttatcca cccacggagc aagtccttta ctatagtatt ggtcgataag atggacatat
     2341 acggcatcca ttccgacaaa tttacttttg ataaaaaagt tgaaataatg gctcaaataa
     2401 aatttgaatg catccggaat gaccttgacc ttagccaaaa caatatccaa agccttaatc
     2461 agagaatcag gctgttgcaa tgtaatattc tggatataat tatcaatttt ctgaaaatat
     2521 acaggggatc ggaagagtct ttcatcgctg aaatccatat tgtccaaata atgatccttg
     2581 tatttattgt attgtcggag cttggtctcc atatcatcat tctgtccgta ttttggaata
     2641 aactgttcct tggatgtttg aattaatttg gcagtaaagg attgcggata cttagaaaca
     2701 taatcatcct gcatcttata tactctttga tcaatttcat ctatcttttc tgccagtttt
     2761 tgcttttcta caggatcttg aataatctgc tgttttgaac gaagggaatc tgccatgggc
     2821 ctttgggagg taagaaaatg gatataattg aaaaatatag tattttcgtc actgttcttt
     2881 accttcaatt tttttgaaag atttgctccg tcagcctcta tactgaaaaa tcgattatcc
     2941 ttatccacta caaactggaa aacatttttc tcaggcaata aaacagccat atacacaccg
     3001 ggatccaaat cattttttcc tgtaaaagaa aagacaccct tcttgcttat tgctgtatcc
     3061 ttgacatatt gcctgtctcc cagataataa cccagcaata aagtatcatt tttataatta
     3121 tttatcttga tttcgatctt ataaccggac tgtgcgaaaa ctgaattcgc taaaagaata
     3181 aaggataaca atagacctgt acgaaacact gacatattta ttactattaa aacagatgta
     3241 aagtaacgca ttggcagcga aattagttct taatgttttg caaaaataag gtcagactct
     3301 ggtagcaagc aggaagagcg ctgccatacg gatagcgaca ccattttcaa cctgttgtaa
     3361 gataatagat gcagggctat cagcaacatc gctggtcaat tcaacacctc tattaattgg
     3421 gccggggtgc ataatgacga tgtttctatt tactttctca agaatctggc gattgattcc
     3481 aaagtatctt gaatattcac gcaaggaagg aaaatatttg gtattctgcc gttctaattg
     3541 aatcctgagc acattagcga catcacacca tttaagtgta tcctctaagt cataggacac
     3601 ttcgactccc aattcggaga tatatcgtgg gatcagtgtt ggaggcccac aaacccggac
     3661 tttggcacct aattttttca ggcagaaaat gttactcaat gctaccctgc tatgaatgat
     3721 atcaccgaca atggcgatat tgcgtccttt gaggtcacca atttgttcca tcatggaaaa
     3781 tgcatcaagc aaagcctggg tgggatgttc atgggttcca tcacccgcat tcagtatcga
     3841 agcatcgatt ttattggcta aagaaatcgc taaagacgga gaagg
//
LOCUS       DAOQMC010000222         3855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12030_length_3855_cov_5.315526, whole genome shotgun sequence.
ACCESSION   DAOQMC010000222 DAOQMC010000000
VERSION     DAOQMC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12030_length_3855_cov_5.315526"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKV31_08420"
     CDS             <1..798
                     /locus_tag="PKV31_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB92923.1"
                     /translation="AMFGIVPKVMWNKMNPADEDNMCSWAMRSLLIETDTRKILIDTG
                     IGQKQGEKFRSHFHPYGKDVLESLSDLGIKSSDITDVFLTHLHFDHCGDAVKIDEQGN
                     YVPTFENAQYWINKKHWEWALESNPREKASFLPENYMPLQEAGVLNLIDFRGENSTNW
                     LEGIRLIGCNGHTRGLTMLDIPYQDTRMIYLTDLIPSVHHVPLPYIMAYDLWPWDTLE
                     EKTAVLEQAVAERHVLFFQHDPVNAVARVRKNEKGRIVAFDISSELY"
     gene            798..2060
                     /locus_tag="PKV31_08425"
     CDS             798..2060
                     /locus_tag="PKV31_08425"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB92924.1"
                     /translation="MESGKETANVYRNREFRIYLATRFLMTFGIQMQNVITGWYLYEL
                     TKDPLILGMVGLSEALPALSSALFAGDLVDKFDKRKVLIIAFSVYLVCGIGFTFLGTQ
                     YAASLSHQLVVWGFYGISMLLGLARAFATPASFSILSYLFPLEMLPKAAPASSTAWQV
                     GAILGPATGGFVYALTDAPSSSFVVCLFMLSSVILASFLSPKPPYEEKKREEQSRRQR
                     ISEGIRYVFSNKVILSAITLDLFAVLFGGATAILPAFASEILHVGAVELGWLRAAPSV
                     GSAILLFLLSIRPPESKSGIKLLLAVTGFGLCMIAFSLSTNLWLSIFFLFASGAFDAV
                     SVVIRSTILQIYTPDHMRGRVSAVNTMFVGSSNEIGAFESGTAARLLGTAPSVLIGGS
                     ITLLVVALVARFAPTLRKLELNTIKLRE"
     gene            2845..>3855
                     /locus_tag="PKV31_08430"
     CDS             2845..>3855
                     /locus_tag="PKV31_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HNB92925.1"
                     /translation="MKIKFAESCQRGFDSTVSIITDQKQIKKVLAKLEIKAKASIIKD
                     QLKENGEMITTIDPQKGKVVFLKIDENKCNGNSSKVIRQTLIKNRNLFGSKITFLLPD
                     GDSESDERWARNVVRACMLSTNYLGKYKFKPNGLPRPQDVVICTISGNVPDAGVLEEE
                     KTIANARLEMMDLINTPSNIKTPEFIADHVARNGKRNGYKVEVFSEKDIKKFNLAALQ
                     SVNNGSDHPPRFVICHYKPKNAGKVKTLGLVGKGVTFDTGGISIKSSTNMHFMKSDMS
                     GAALVLTFTDLCARLQLPVEVITAVPLTENMVDGSSTKPGDVVGSYIGKSIEVIDTDA
                     EGR"
BASE COUNT         1148 a          819 c          808 g         1080 t
ORIGIN      
        1 gccatgttcg gtatcgtccc aaaagtcatg tggaacaaga tgaatccggc cgatgaagac
       61 aacatgtgtt cctgggcaat gcgcagcctc ctgatcgaaa ccgatacaag gaaaatcctc
      121 atcgacaccg gtattggtca aaagcaagga gaaaaattcc gctcccactt tcatccctat
      181 gggaaggacg tcctggaaag cctttctgat ctcggaatca agtcaagcga catcacagat
      241 gtatttctaa cgcatctgca ttttgatcac tgtggtgatg ctgtcaagat tgacgaacaa
      301 ggtaattacg tccccacctt tgaaaatgcc caatattgga tcaacaaaaa acactgggag
      361 tgggcacttg aatccaatcc gagagaaaaa gcttccttcc tccccgaaaa ctatatgcca
      421 ctacaggagg ccggagtact caacctgatt gattttcggg gtgagaattc taccaattgg
      481 ctggaaggta tccgattgat tgggtgtaat ggtcacaccc gtggactcac catgttggat
      541 atcccctatc aagataccag aatgatttac ctcaccgatc ttattccatc cgtccatcac
      601 gtgccactcc cttacatcat ggcctatgat ctttggccct gggacacctt agaggaaaaa
      661 acagctgtac tggaacaggc cgttgctgaa agacatgtcc tgtttttcca gcacgatcct
      721 gtcaatgcag tagcgcgtgt ccggaaaaat gaaaagggaa ggattgtagc cttcgatatt
      781 tcttccgaat tgtattgatg gaatccggca aagaaacagc caacgtatat agaaacaggg
      841 agtttaggat ttaccttgcg acacgttttc taatgacctt cggcatccag atgcagaacg
      901 tcattacagg ctggtacctg tatgaactaa caaaggatcc gctcatatta ggtatggtag
      961 gcctctctga agcattgccg gcactttcct ctgcattgtt tgccggtgat ctggtcgata
     1021 aatttgataa aagaaaggta ttgattatag ccttcagtgt gtacctggta tgtggtatcg
     1081 gatttacatt tttaggcaca caatatgcag catccctatc ccatcagctc gtagtttggg
     1141 gattctatgg catttcgatg ttgctggggc tggcaagggc tttcgcgact ccggcatcct
     1201 tttccatcct atcctatctg ttccctctcg aaatgcttcc aaaggcagct ccggcaagca
     1261 gtactgcctg gcaggttggt gccatccttg gtcccgctac aggcggcttc gtatatgcac
     1321 tcactgatgc tcccagctcc tccttcgtgg tatgcctttt tatgttgagt agtgttatcc
     1381 tggcaagctt cctgtcacct aaacctccct atgaagaaaa aaagagggag gaacagtcca
     1441 gaaggcagag aatcagcgag ggaatcagat atgtattcag taacaaagtc atactttcag
     1501 ccatcaccct ggacttattt gctgttcttt ttggtggtgc aacggcaatc cttccagcct
     1561 ttgccagtga aattctacat gtaggagctg tcgagcttgg gtggttgaga gctgcacctt
     1621 ccgtcggatc cgctattttg ttatttcttc tgtcaatcag gcctcctgaa agtaaatccg
     1681 gaatcaaact actgttggca gtaaccggat ttggattgtg catgattgcc ttcagcctct
     1741 caaccaatct ttggttatcc atattttttc tatttgccag tggagccttc gatgctgtca
     1801 gtgtagtgat ccgaagcacc attcttcaga tatatacccc tgaccatatg cgtggccgtg
     1861 ttagtgcagt caatactatg tttgttggat cttccaatga aatcggtgcc tttgaatcag
     1921 gtactgcagc aaggttactt ggaacagcac cttctgttct tattggtgga tccattacat
     1981 tgctcgtggt ggccctggtt gccaggttcg ctccaacgct cagaaaattg gagcttaata
     2041 caatcaagtt gcgagaataa ggcgtgctta caacgaatag ctccaaatcg ataaagcatc
     2101 tgataagaac tagttcagtg tagacctgtc caatattgca cttacaaatt ataccaagat
     2161 gatttgtata attgcccaca aatttttggt attaattgga aaataagcaa aatgaaagat
     2221 tgccccatat caattgttcc ttaggaaaag attaatcggt gcctaatcaa aaaaacagcg
     2281 tattaaattg ataataacct gatgctctag ggcggtttgc cggcttaaca cttgtttgat
     2341 tcaagaaaga ccggacattg aatctgacta atgacaaagc tgctaatagc aaagttttaa
     2401 agagcagcgt gaagtttcct aagtttttat taccccatta agtatactat tcaactttaa
     2461 ttaattgacc tattccataa tatattttaa tttatattaa tatataaaat aaatattatc
     2521 tatacattat cactttttca taacccgtaa aatcttttat aatccacctg ttcttcccgg
     2581 cagaagaaag gattcatttt attcagctca cattgaggaa tatattttat ctcaactgat
     2641 attttttcca taatttgcac cattattaag gttaaaaatg tattaaacag gctgaataca
     2701 aaattttctt tcggtataag ggccggaatc ctttcggtta aatgtttaga tcatcattta
     2761 cagaccggat ataaaaatcc attaccctaa tagtcaatct gttaaagtca gaattatcag
     2821 gattttaaaa caaagaaaaa ttagatgaaa attaaatttg cagaatcttg ccaaagagga
     2881 tttgactcta cagtttcaat catcactgat caaaaacaaa tcaaaaaggt cttggccaaa
     2941 ttggagatta aagcaaaagc ttccattatc aaggaccaat tgaaggaaaa tggggagatg
     3001 ataaccacca ttgacccaca aaaaggtaaa gtcgtcttcc ttaaaataga tgaaaacaaa
     3061 tgcaatggca atagcagtaa agtaatcagg cagacactta tcaaaaacag gaatctgttc
     3121 ggttcgaaaa tcactttcct attaccggat ggcgattcgg aatccgatga acgatgggcc
     3181 cgaaatgtgg taagggcgtg catgctgtca accaattatc ttggaaagta taaatttaaa
     3241 cctaatggat taccccggcc acaagatgtt gtgatttgca ctatttcagg caatgtgcct
     3301 gatgcgggag tcctggaaga ggaaaaaacc atagccaatg cacggttgga gatgatggac
     3361 cttattaata caccttccaa cattaagact cccgaattca ttgcggacca tgtcgcccga
     3421 aacggtaaaa ggaatggcta caaagtggaa gtcttctctg aaaaagatat taaaaagttt
     3481 aatcttgcag cacttcaatc agtaaacaat ggcagtgatc atccgcccag attcgtcatt
     3541 tgccattata aacccaaaaa tgcaggtaag gtaaagaccc ttgggctcgt cggcaaaggc
     3601 gtaactttcg ataccggcgg cataagcatc aagagttcaa ccaatatgca ttttatgaag
     3661 agtgatatgt cgggagcagc tttggtactt acatttaccg atctatgtgc ccggctccaa
     3721 ctgcctgtcg aagtgataac ggcagttcct ttgacagaaa acatggtgga cggcagttcg
     3781 accaaaccgg gtgatgtagt aggcagttat attggcaaaa gcatagaagt catagatacc
     3841 gatgcagaag ggagg
//
LOCUS       DAOQMC010000223         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12064_length_3849_cov_4.884555, whole genome shotgun sequence.
ACCESSION   DAOQMC010000223 DAOQMC010000000
VERSION     DAOQMC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12064_length_3849_cov_4.884555"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PKV31_08435"
     CDS             <1..1188
                     /locus_tag="PKV31_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92926.1"
                     /translation="QSSRGGSILKNVKDKTALNIFFVEEIKSSSPGSVLGFFSPENDY
                     LVVRNSEANYESQTTSHEMGHFFSLRHTFYGWECEPWNVNDHGQIVTFTIAPCFSAQV
                     ELVNGSNCSTAADKVCDTPPDYNFGFGWPNDCRPFNVDVYDKNNDLIRPSQNNFMSYF
                     FGCTGWHFSQGQKNLIRTDLNSSKRSYLKSTYTPPSTTVSGEVNYQYPINNQEALRNA
                     PIVLQWENVPGATFYIVEYSRRKSFDSNFTVTEMVRTNQVTIPANYFPSGAATGYWRV
                     IPMNEAVTCNVPANGSEQSFTLVNALGTKSIDGLQHFVVKPNVLRSGNSIQVEVSAGK
                     SMDCMVQITDINGRVVYHYGQYKMNLGMNKIVLDEINLSTGIYIVSIKNKSGINSQKL
                     IVL"
     gene            1319..2524
                     /gene="sucC"
                     /locus_tag="PKV31_08440"
     CDS             1319..2524
                     /gene="sucC"
                     /locus_tag="PKV31_08440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNB92927.1"
                     /translation="MNLHEYQGKELLAGYGVAVQRGFVAENVDQAVEAFKRVNEELGS
                     QFAVVKAQIHAGGRGKGGGVKLVKNLDELKAEAGRIIGMYLRTPQTPGGMEGPGKLVR
                     KVFIAEDSYAPDFAACKEFYVSVLLDRGTGKNVIVYSTQGGMDIEKVAEETPELVLKE
                     MVDPTLGLLPFQARKIAFNLGLSGKAFKEFVVFIDRLYKAYVGSDASLFEINPLIKTG
                     DDRLVAVDAKVSIDDNALYRHKDLEEAYDKLEEDPADLEAKDADLNYVKLDGNVGCMV
                     NGAGLAMATMDMIKLIGGSPANFLDVGGTADAARVEKALNLILKDPSVKAILINIFGG
                     IVRCDRVAGGVIEAYKNIGNIPVPIIVRLQGTNAEIAKKMIDESGLKVLSAIELKEAA
                     EQVSKALAG"
     gene            2580..2981
                     /locus_tag="PKV31_08445"
     CDS             2580..2981
                     /locus_tag="PKV31_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92928.1"
                     /translation="MKKLILVLCIIIGIGGMFLPWFSLPLIESPSGIELGYSWLGLAL
                     MGINLIPVFLASFDKKSSGIWASLILVSGLLACGYGLLNYFSYKSDILDMGDGNFIAK
                     AVSDTISLGAGYIMYMAAGLVIFLIGLLSKK"
     gene            complement(2988..>3849)
                     /locus_tag="PKV31_08450"
     CDS             complement(2988..>3849)
                     /locus_tag="PKV31_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNB92929.1"
                     /translation="SRLEHHCVSHTLTYLQRNHGTEVILLDNDEYGRIDLIQLEEYLA
                     NSPDKKLVSLMHANNEIGTVADIEAIGNLCRQYDALFHSDTVQTVGHFPLDVSKINIH
                     FLTGTGHKIHGPKGSGFLYIRSGHILEPLIDGGSQEKNLRGGTENLHGIIGLAKSMEL
                     AVNHLPERRTYITGIKNYFIEQLRQHIPEVDFNGDLSEDSLYSVLNVSFPAHPKNEML
                     LLHLDIHGVSVSGGSACSSGADRGSHVIEALDKDPMRKAVRFSFSHHNTFEEVDKVVR
                     MIRSIYYGNA"
BASE COUNT         1072 a          721 c          910 g         1146 t
ORIGIN      
        1 caaagttcaa ggggtggaag tatcctgaag aatgtaaagg ataaaacagc attaaatatc
       61 ttttttgtag aagaaatcaa atccagcagc ccgggaagcg ttcttggatt ttttagtccg
      121 gagaatgatt accttgtcgt tagaaacagt gaggctaatt acgaatctca aaccacttca
      181 catgagatgg gacacttttt cagccttcga catactttct atggttggga atgcgaaccg
      241 tggaatgtaa atgaccatgg ccaaatagtc acttttacga ttgccccttg ttttagcgct
      301 caggttgaat tagtcaatgg ttccaattgt tctacagcag cagataaggt ttgtgatact
      361 ccaccggact acaactttgg ctttggctgg cctaatgatt gcagaccatt caatgttgat
      421 gtatatgaca aaaataatga cctgatcagg ccctctcaaa acaactttat gtcatacttt
      481 tttggttgta caggttggca tttctctcag ggtcagaaaa accttatcag gactgatctg
      541 aatagttcga agcggagtta cctgaaatct acatacacac caccctcaac taccgtgagt
      601 ggtgaagtaa actatcagta tcctattaat aaccaggaag cgttaagaaa tgctccaatc
      661 gtacttcaat gggagaatgt tcccggggct actttttata tcgtagaata ttcaagaaga
      721 aagtcatttg attctaattt tactgtaaca gagatggtaa ggaccaatca agttacaatt
      781 cctgccaatt attttccatc cggagctgca acaggttact ggagagtaat accgatgaat
      841 gaggccgtta cttgcaatgt tccggctaat ggaagcgagc aatcttttac ccttgtcaat
      901 gctttaggta ctaaaagtat tgatgggctg cagcattttg ttgtcaagcc caacgtctta
      961 cgctccggca attccattca agttgaggtt agtgcaggca aatccatgga ctgtatggtt
     1021 cagattactg acattaacgg tagggtagtc tatcattatg gacagtataa gatgaattta
     1081 ggtatgaata agattgtctt ggatgagatt aatctgtcta ccgggatcta tatcgtcagt
     1141 attaagaaca aatcaggtat caactcgcaa aagttgattg tactttaatc aagatgtatg
     1201 ataatgcccc gggataattt ttggggcact gttatttaat gacatttggg ttccttttat
     1261 ctgaatatac ttttgtatca ttgcagcatt atattaaatc agttataaac aatatacaat
     1321 gaatttacac gaatatcagg gaaaagagtt gcttgccgga tacggcgtgg ctgttcagcg
     1381 aggatttgta gcagagaatg tcgatcaggc tgtcgaagca tttaaaaggg tcaatgaaga
     1441 gttgggttca cagtttgctg tggtgaaggc tcagattcat gccggtggcc gaggaaaggg
     1501 tggtggtgta aagctggtca aaaatctgga tgagttgaag gcagaagccg gccgtatcat
     1561 cggtatgtat ctgcgtacac ctcagactcc cggaggcatg gaaggtcccg gaaaactggt
     1621 tagaaaagtt tttatagcag aggactccta tgcaccggat tttgctgcat gtaaggaatt
     1681 ttatgtttct gttttacttg acagagggac cggaaagaat gtgatcgttt attcaacaca
     1741 agggggaatg gatatagaaa aagtggcaga agaaactccg gagttggttt tgaaggagat
     1801 ggtagatcct acgttgggac ttcttccatt tcaggcaaga aagattgcct ttaatcttgg
     1861 attgtcaggt aaggctttca aagaattcgt tgtattcatt gatcgcttat ataaggctta
     1921 tgtaggctcc gatgcaagtt tgtttgaaat taatccgctg attaaaacag gagatgatcg
     1981 tttggttgct gtcgatgcaa aggtctccat tgacgataat gctctgtaca gacataaaga
     2041 tctggaggag gcttatgata agctggagga agatccggca gaccttgagg ctaaggacgc
     2101 cgatctcaat tacgtcaagc tggatggaaa cgttggctgt atggtcaatg gagcaggtct
     2161 ggccatggct actatggata tgatcaagtt gataggaggc tctccggcca acttcctgga
     2221 tgttggaggt actgcagatg ccgcgagggt ggaaaaagcg cttaatctca ttcttaagga
     2281 tccttccgta aaggccatcc tgatcaatat tttcggaggg atcgtgcgct gcgaccgtgt
     2341 tgccggaggc gttatagaag cctataaaaa tattgggaat attcctgtac caatcatcgt
     2401 ccggcttcag ggcaccaatg ccgagattgc taagaaaatg atcgacgaat cagggctaaa
     2461 ggtactttct gccatcgaat taaaagaagc tgcggaacag gtatctaaag ctctggcagg
     2521 ttaaaaataa aggccggatc tatttccggc ttttttttcc ttcaaatttt tgaattgaat
     2581 tgaagaagtt aattctggtt ttatgtatca tcattggtat aggaggtatg ttcttgccct
     2641 ggttttcttt gccactgatt gaaagccctt caggaatcga actgggatat tcctggcttg
     2701 gattggcatt gatgggtatc aatttaatac cggtattttt agcgtcattt gataaaaaat
     2761 cttccggaat ttgggcttcc ttaattttgg tatccggcct gctagcctgc ggttatggac
     2821 tgttgaatta tttcagttat aagtcagata ttttagacat gggtgatggc aatttcattg
     2881 caaaggcagt atctgacacc atttccttag gtgccggtta tattatgtat atggctgcag
     2941 gactggttat tttcctgata ggattacttt cgaagaaata agtcgcctta agcatttcca
     3001 taataaatgg atctgatcat cctaacgacc ttgtccacct cttcaaatgt attgtggtga
     3061 gagaacgaga atcgcacagc cttcctcatt ggatccttgt ctaaagcctc gatgacgtgg
     3121 ctacctcggt cagcacctga agagcatgca cttcctccgg agacagagac gccatgaatg
     3181 tcgagatgca aaagcaacat ttcgtttttg ggatgcgccg ggaaagaaac attcagtacg
     3241 ctataaagcg aatcttcact gaggtcacca ttgaaatcca cttccggaat atgttgtctt
     3301 aactgttcga taaaataatt cttgatgcct gtaatgtagg tacgtctttc cggcaaatgg
     3361 ttgacagcaa gttccataga ttttgccaga cctatgatgc catggaggtt ttcagtgcct
     3421 ccccggagat ttttctcttg cgaaccacca tcaatcaatg gttcgagaat gtggccggaa
     3481 cggatataca gaaatccgct gcctttcggg ccgtggattt tgtgacctgt tcctgtcagg
     3541 aaatggatat taatcttaga cacgtctaaa gggaaatgac ctactgtttg cactgtatcc
     3601 gaatgaaata gtgcatcata ctgacggcaa agattgccaa tggcttcaat atctgcgact
     3661 gtaccaattt cattgttggc gtgcataagg gagaccagtt ttttatccgg gctattggcc
     3721 agatattctt ccagttggat caagtctatt ctgccatact cgtcgttgtc gagtaagatg
     3781 acttctgtgc catgattcct ttgcagataa gtgagtgtat ggctgacgca atgatgctcc
     3841 aatctggaa
//
LOCUS       DAOQMC010000224         3813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12250_length_3813_cov_6.179351, whole genome shotgun sequence.
ACCESSION   DAOQMC010000224 DAOQMC010000000
VERSION     DAOQMC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12250_length_3813_cov_6.179351"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKV31_08455"
     CDS             <1..324
                     /locus_tag="PKV31_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92930.1"
                     /translation="IQIIQYTKFWSIYFLTFVLVNKPNTTKIQARVQYAPESVLLLTS
                     QIAQRSQHAPESNVRTLQNAKRSISSIHIFFEISQERGSTCSGICLMLIELSGWAAYA
                     GMSEC"
     gene            complement(395..1810)
                     /locus_tag="PKV31_08460"
     CDS             complement(395..1810)
                     /locus_tag="PKV31_08460"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNB92931.1"
                     /translation="MKSITVFEIIKVGIGPSSSHTMGPWNAAEMFLDLIREKKDIASV
                     REIFVEFFGSLAKTGIGHGTDIAGMLGLSGENFKTIDTSQIMEKADVIKSEGKIILGG
                     EKEIPFVYGKNLVLNKDKSLPFHPNGMIFKAIFNDGSELSQDYYSVGGGFVATQEEVS
                     IENYCVRTLHPCHHGDDILKYCNQLGVSVSDLIYINEESWQSREETNRKALEIWHHIK
                     ECIYHGVNTEGILPGGLNVVRRAAGLNRKLLGDKVYRNMEEWYNLIVDSEVAFTEINK
                     WISCFALAVNEENASFGRIITAPTNGASGVIPAVLMYSQAFTKYTGDDNIIRFLLVAG
                     EIGTLFKKNATISAAMGGCQAEIGVSSAMAAAGLTEIMGGTPAQVLMAAEISMEHHLG
                     LTCDPVGGLVQIPCIERNSMGAIKAITAANIAIESDPSKARVTLDEVIQSMWETALSM
                     NDRFKETSEGGLAIAVNIAEC"
     gene            2301..2387
                     /locus_tag="PKV31_08465"
     tRNA            2301..2387
                     /locus_tag="PKV31_08465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2335..2337,aa:Leu,seq:cag)
     gene            complement(2453..3712)
                     /locus_tag="PKV31_08470"
     CDS             complement(2453..3712)
                     /locus_tag="PKV31_08470"
                     /inference="COORDINATES: protein motif:HMM:NF028181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HNB92932.1"
                     /translation="MFIKKLLILYFSFFIGISVYGQQVLCAKIPGYTGYSIPNDKGIV
                     FDELLGCTQWTNENLTLQYNMQIEEKGDLILKFMLSNDGPSPAYISVKFGDKTREVKV
                     PPTGSHIQYILVDAGIFTVDYPGFYSVWIKPISKSGQYFPGIMNVQLCAPFADKINFP
                     TIPDRNAPTVNLTYLNPQKKKVSGMVVNALVPNNFDYTGTRIAAIGNEVFKVGMANET
                     KGRYIYLSWKKVKGYEIPQFQYSQFKSFFIDSSNEKVNRLVIPYNWSPDQPIAFSLSL
                     REDTCTGEHFWEAQVFNDKKKRWHTIGIVSGGKMDNLIKDWNSTIVNSDPNTGNVEHK
                     ALFSNQYFILADGSKYQVAKARFGHDVKGKKERKDYGAGIVNNSFWLSTGGFSFSQAT
                     YGRIYEVKLKPGVPSNEIFLASFDSAK"
BASE COUNT         1074 a          783 c          743 g         1213 t
ORIGIN      
        1 atacaaataa tccaatacac caaattttgg tctatttatt ttttaacttt cgtattagtt
       61 aataaaccaa atacaaccaa aatccaagcc agggttcaat atgctccgga atcagtcttg
      121 cttctgacct cacaaattgc ccagaggagt caacatgctc cggaatccaa tgtgcgcacg
      181 ctacaaaatg ctaaacgcag catttcatca atccacattt tctttgaaat ttcacaagaa
      241 agggggtcaa catgctccgg aatttgcctc atgcttattg aactttcagg gtgggcagct
      301 tatgccggaa tgtcagaatg ttgaaagatt ataacttgtt tctgagattt cttttcttct
      361 aaatttgggg tcagcttatt ccggaatatc catattagca ttctgctata ttcactgcta
      421 tggctaagcc tccctcggat gtttccttga agcggtcatt catactaagt gctgtttccc
      481 acatagattg aatgacttca tctaaagtga ctcttgcttt ggagggatca ctttctatcg
      541 ctatattggc tgcggtgatg gctttgatgg cgcccatcga gtttctttca atacacggga
      601 tctgcacaag gccgccaacc ggatcacagg tcaatccaag atgatgttcc atagatattt
      661 ctgccgccat gagtacctgt gcaggtgttc cacccatgat ttctgtaaga ccggctgcag
      721 ccattgctga tgaaacaccg atctcagctt gacacccacc cattgctgct gatattgttg
      781 cgttcttctt aaacagggtg cctatttcgc cggctactag taagaatcgg attatattgt
      841 catcgcccgt atatttggta aatgcctgag aatacatcag tacagcgggt ataacaccac
      901 tagccccatt ggttggtgct gtgatgatgc ggccgaaact ggcattttct tcattgaccg
      961 caagagcaaa gcaagaaatc catttattaa tttcagtgaa tgcaacctct gaatctacaa
     1021 tgagattata ccattcctcc atatttcggt acaccttgtc gccaagtagt ttgcgattta
     1081 gtcctgctgc acgccttacc acatttagcc ctcccggtag gatgccttca gtatttactc
     1141 catggtatat acactccttg atatggtgcc agatttcaag cgcctttcga ttggtttctt
     1201 cccgactttg ccagctttct tcattgatat agatcaaatc tgaaacagaa actcccaatt
     1261 ggttacagta tttcaaaata tcgtcaccgt gatggcaagg atgcaaggtg cgaacacaat
     1321 aattttcgat ggatacctct tcctgtgtag ctacaaatcc tcctcccaca gagtaataat
     1381 cctgagataa ctcgctgcca tcattgaaga tagctttgaa gatcattcca ttgggatgga
     1441 agggcaggct tttgtcttta ttgagtacga ggtttttacc gtacacaaag ggaatctcct
     1501 tttctccacc cagtataatt ttaccctctg atttgataac atcagctttt tccataatct
     1561 gcgatgtatc gattgttttg aagttttctc ccgataatcc cagcattccg gcaatatcgg
     1621 taccatggcc gattcccgtc ttggcaaggg aaccaaaaaa ttcaacaaat atttccctca
     1681 ctgatgctat gtctttcttc tccctgataa gatcgagaaa catctcagcg gcattccagg
     1741 ggcccatggt gtgtgaactg gatgggccta ttcccacttt tataatttca aaaacagtta
     1801 ttgatttcat cttaatttta atataaatga tgatttggtt gatgacaaat ctacatcaat
     1861 tcaaaagtat aacatagcaa tttatgaata tattcaacaa caatacagag tttgattgag
     1921 ttatagcggg ttgttatcgg catgtttgat ctgggtgcgt tgaataatcc gaattgaaaa
     1981 attggaaaaa aaatgatact tcgtgcagcg tttttttgaa tgaatgaata tatagatata
     2041 taacgatatt tttcttactc attttcctaa aaaaatacag gaatatagtc aatattataa
     2101 aagccttccg ttgggaggct ttttattttt ccgcctgatt cctttcagta tgcttgttag
     2161 gttgcgagtg cgttattttc aatatattat gcaatttggg gtaattccag ggcaaattgt
     2221 atggttgttt tatggataat taagtaaaaa aatgatgttg tttttaaaaa ttatgattat
     2281 tcttgcatcc taaatcagtg gcccaagtgg cgaaattggt agacgcactg tgttcaggtc
     2341 gcagcgttcg aaaggacgtg ctggttcgaa tccggtcttg ggcaccagtt ttaacaaagc
     2401 gtaggaagtg tttcctgcgc tttatttttt ttatgttggt tattgaatta atttacttgg
     2461 ctgaatcgaa gcttgctaaa aaaatttcgt tgcttggcac accgggtttt aacttaactt
     2521 catagatgcg accataggtt gcctgagaaa aagagaagcc acctgtactt agccagaatg
     2581 agttgttgac aatacccgct ccataatcct tgcgttcttt ttttcctttt acatcatgtc
     2641 cgaagcgtgc ttttgcaacc tgatactttg aaccatccgc cagtataaaa tattggtttg
     2701 aaaaaagagc cttatgttct acattgccgg tatttggatc tgaatttacg atggtgctat
     2761 tccagtcctt gatcaggttg tccattttac ctccactaac aataccaatg gtatgccatc
     2821 ttttcttctt gtcattgaag acttgagctt cccagaaatg ttcccctgta caagtatctt
     2881 cgcgaagtga aagtgagaat gcaatgggtt gatcaggact ccagttgtat ggaataacca
     2941 atctattaac cttttcatta gaagaatcaa taaaaaagct tttgaattgg ctatattgga
     3001 attgaggtat ctcgtagccc ttgacttttt tccaggataa ataaatatac ctacctttgg
     3061 tctcattggc catgccaact ttgaaaacct catttcctat cgcagcaatc ctggttcccg
     3121 tataatcgaa attgtttggt acaagtgcat ttacaaccat gccgctgact ttcttctttt
     3181 gaggattaag ataggttaga tttacagtag gggcattgcg gtctggtata gtcgggaaat
     3241 tgattttatc tgcaaacgga gcgcacagct gaacattcat gatgcccggg aagtattggc
     3301 cggatttgga tatcggcttg atccacacag aataaaaacc gggataatcc acggtgaata
     3361 ttccggcatc aaccagaata tattggatat ggctgcctgt aggagggact ttcacctctc
     3421 gggtcttgtc accaaatttc acactgatat aggcaggaga gggcccgtca ttcgacaaca
     3481 taaatttcaa tataagatca cctttttctt cgatctgcat attatactga agtgttagat
     3541 tttcattggt ccattgcgta cagcccaata attcatcgaa gacgatgcct ttgtcgtttg
     3601 gaatagaata gccggtatag cccggtatct tagcacaaag tacctgctga ccgtagacag
     3661 aaatgcctat gaaaaaggag aagtataaaa tcagcagttt ttttataaac atatttccaa
     3721 accgatatct tgcaaacagt gaaaatttac attaagagca ggcctaaaag ctgacaaaaa
     3781 tacgtaaatg gaattatggt tcaaagctaa aaa
//
LOCUS       DAOQMC010000225         3777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12459_length_3777_cov_4.389575, whole genome shotgun sequence.
ACCESSION   DAOQMC010000225 DAOQMC010000000
VERSION     DAOQMC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12459_length_3777_cov_4.389575"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /gene="map"
                     /locus_tag="PKV31_08475"
     CDS             <1..717
                     /gene="map"
                     /locus_tag="PKV31_08475"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNB92933.1"
                     /translation="RPGVSGKFLDKEAETLINDLGGKPAFKGYNGFPASLCISVNDAV
                     VHGIPRDKELQEGDVVSVDCGAVMNGYFGDSAYTFALAPVSDDITKLLRITNESLYKG
                     VEQARVGNRIGDIAFSIQEYTEIKHGYGVVRELVGHGIGKNLHEAPDVPNFGRRGNGL
                     CLEENWTIAIEPMINMGTKHVYQAPDRWTILTKDGKPSAHFEHTVAVKPQGGMILSDH
                     RPIVEEIKNNPNIVNISENI"
     gene            745..963
                     /gene="infA"
                     /locus_tag="PKV31_08480"
     CDS             745..963
                     /gene="infA"
                     /locus_tag="PKV31_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNB92934.1"
                     /translation="MSKQDIIKQDGIIEEALSNAMFRVRLENDHQILATISGKMRMHY
                     IRILPGDKVSVEMSPYDLSRGRIIYRYK"
     gene            979..1095
                     /gene="ykgO"
                     /locus_tag="PKV31_08485"
     CDS             979..1095
                     /gene="ykgO"
                     /locus_tag="PKV31_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNB92935.1"
                     /translation="MKVRASIKKRSADCKIVRRKGKLYVINKKNPKFKQRQG"
     gene            1124..1501
                     /gene="rpsM"
                     /locus_tag="PKV31_08490"
     CDS             1124..1501
                     /gene="rpsM"
                     /locus_tag="PKV31_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNB92936.1"
                     /translation="MARIAGVDLPKNKRGVIALTYIFGIGRSLAGKVLAQAGVSEDTK
                     VIDWTDDNIKTISRIVNDEHKTEGQLRSEVQLNIKRLMDIASYRGLRHRKGLPLRGQR
                     TKNNSRTRKGRRKTVANKKKATK"
     gene            1535..1924
                     /gene="rpsK"
                     /locus_tag="PKV31_08495"
     CDS             1535..1924
                     /gene="rpsK"
                     /locus_tag="PKV31_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNB92937.1"
                     /translation="MAKAQKKVKKRKVKVESEGLAYIQASFNNLIISLTNRQGEVISW
                     ASAGKAGFRGSKKNTPYAAQVAATDAGKVAYDAGLRNVEVYVKGPGAGRESAIRAIDG
                     AGIKVTRIFDVTPIPHNGCRPPKKRRV"
     gene            1956..2567
                     /gene="rpsD"
                     /locus_tag="PKV31_08500"
     CDS             1956..2567
                     /gene="rpsD"
                     /locus_tag="PKV31_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNB92938.1"
                     /translation="MARYTGPATKKARAFGEAIYGPDKAFDKKKYAPGQHGASRKRRQ
                     KSDYALQLMEKQKAKYTYGVLERQFRNTFTKAARKGGVTGTVLLQFLESRLDNVVYRM
                     GIASTRREARQLVSHRHVVVNGVVTNIPSFHVRPGDVVEVKGKSRGMDIILSNVKSKH
                     DHRKYAWIEFNPERMEGKFLAYPEREQIPENINEQLIVELYSK"
     gene            2617..3615
                     /locus_tag="PKV31_08505"
     CDS             2617..3615
                     /locus_tag="PKV31_08505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNB92939.1"
                     /translation="MSILNFQKPDKIILQKSTDFDGVFEFKPLEPGFGQTIGNSLRRV
                     LLNSLEGFAISAIRIGGVDHEFSTIPGVVEDVIEIILNLKQVRLKLQMKDEDIQNEKI
                     YLTLRGKEQFLSGDIEQFTNVFKVMNPDLLICQMEPSVTLEIELTITKGRGYVAADEQ
                     PERESVIGVIPVDSIYTPVKNVAYQVSNTRVGQRTDYEKLTIEVKTDGTIHPEDAVKE
                     ASRILIQHLMLITDENISFDDAAKGEEDVVDEHILHMRKLLKTSLEDLDLSVRAYNCL
                     KAAKINSLGEMVQYETHELLKFRNFGKKSLVEIEELLSTKGLHFGMDLSKYKLEEE"
BASE COUNT         1209 a          682 c          892 g          994 t
ORIGIN      
        1 aggcccggag tgtccgggaa gtttttggac aaagaagcag aaacactcat caatgacctg
       61 ggcggcaagc ctgcattcaa agggtataat gggtttcctg cttctttgtg tatttcagtg
      121 aatgatgccg tagtacatgg aatacccagg gataaggagt tgcaggaagg tgatgtggtg
      181 tcggttgatt gcggggcagt gatgaatgga tattttggag actccgcata tacttttgcc
      241 ctggcacctg tcagcgacga cattactaaa ttactgcgta tcacgaatga atccctgtat
      301 aagggtgtag agcaggcaag agtaggaaat cgtatcggtg atatagcctt ttcgattcag
      361 gaatataccg agataaagca cggttatggt gtcgtaaggg agttggtggg ccatggtatt
      421 ggaaaaaatc tccacgaagc acctgatgtg ccgaactttg gacgaagggg gaacggattg
      481 tgcctggaag aaaactggac cattgcaatc gaaccgatga tcaatatggg tacaaagcat
      541 gtctatcagg ctccggatcg ctggaccata ctcactaagg atggcaagcc ttcagcccac
      601 ttcgaacata cagtagcagt gaagcctcaa ggtggtatga tactgtcaga tcacagaccg
      661 attgtggaag aaataaaaaa taacccaaat attgtgaata tttcagaaaa tatttagata
      721 tttgcactcc gaaaaaaagg acgtatgtca aaacaggata taatcaagca ggacggtatt
      781 attgaggagg cgttgtcaaa cgccatgttc agggtacgcc ttgagaatga tcatcagatt
      841 ttggcaacca tttcgggaaa aatgagaatg cactatatac gtattctgcc cggtgataag
      901 gtttctgtgg agatgtctcc ttatgatttg tcccgtggaa gaatcattta tagatataaa
      961 taattaaaat tttgactgat gaaagttaga gcatcaatta aaaaaagatc tgcggattgc
     1021 aagattgtca gaagaaaggg taagttgtat gtaatcaaca aaaagaaccc taagtttaaa
     1081 caaagacaag gataatattt agcaattata atatagtaat tatatggctc gtatagcagg
     1141 agttgattta ccaaaaaaca aaaggggtgt catcgccctt acttatattt ttggcatagg
     1201 ccgttcactg gctggtaagg tgcttgcgca ggcaggtgtg agtgaagata ctaaagttat
     1261 tgactggaca gatgataata tcaagaccat ctccagaatt gtcaatgatg aacacaagac
     1321 tgaaggccag ttgaggtctg aagtgcagct taatatcaag cgattgatgg atattgccag
     1381 ttaccgtggt ttgcgccatc gtaaaggctt gccattgaga ggccagagga ctaagaataa
     1441 ctccagaaca cgtaaaggaa gacgtaaaac tgttgctaac aagaagaagg caaccaaata
     1501 ataataaata acattgtttt tatagcttat cattatggct aaagcacaaa agaaagtaaa
     1561 gaaaagaaaa gttaaagtcg agtctgaagg actggcttat attcaggcaa gctttaataa
     1621 cctgattatt tctttgacca acagacaagg agaagtgatt tcctgggcat ctgccggtaa
     1681 ggccgggttc agagggtcta aaaagaacac tccttacgct gcacaagtcg cagctactga
     1741 tgcaggcaaa gtggcttatg atgcagggct tagaaacgtt gaggtgtatg tcaagggacc
     1801 tggagcggga agagaatccg ctatcagagc catcgatgga gcgggtatta aggttaccag
     1861 gatttttgac gtaaccccca tcccacacaa tggatgccgc cctcctaaga aaagaagagt
     1921 ttaatcacta ttattttata aagtagaact atattatggc aagatataca ggtcctgcaa
     1981 ccaagaaggc aagagctttt ggtgaggcta tttacgggcc ggataaggcc tttgataaga
     2041 aaaaatatgc tcccggccag catggtgctt ccagaaagag aaggcaaaag tctgattatg
     2101 cacttcaatt gatggaaaaa caaaaggcaa aatataccta tggtgtattg gagcgtcagt
     2161 ttagaaatac attcaccaaa gctgccagaa aaggcggtgt gaccggtacc gtgttactac
     2221 aattccttga atccagactg gacaatgttg tttacaggat gggaatcgcc agtacacgtc
     2281 gggaagctcg tcagttggtc tcacatagac atgttgtagt taatggcgtt gtaacaaata
     2341 ttccatcctt ccatgtcaga cccggagatg ttgtcgaggt aaaaggtaaa tccagaggta
     2401 tggatatcat cctttccaat gtcaaatcca agcatgacca ccgtaaatat gcctggattg
     2461 aattcaatcc tgaacgtatg gagggaaagt tcctggctta tcctgaaaga gagcaaattc
     2521 ctgaaaacat caatgaacag ttgatcgtcg aattgtattc caagtaattt tgtattcttt
     2581 tatcaagtct tttcttcatc tatagatttt ttcaaaatga gtatattaaa ttttcaaaaa
     2641 cctgataaaa ttatacttca aaaatccact gacttcgatg gtgtttttga atttaaaccg
     2701 ttggaaccgg gcttcggtca gacaatcggt aactcattga gaagagtact cctcaactct
     2761 ctggaaggtt tcgctatttc agcaatcaga attggcggtg ttgaccacga attttcaacc
     2821 attcccggag ttgtggaaga tgtcattgag atcatcctga atcttaagca ggtccgtttg
     2881 aagctgcaaa tgaaggatga agatattcaa aacgaaaaaa tttacctcac tctccgcggt
     2941 aaggaacaat tcctttccgg tgacattgaa cagtttacca atgtgttcaa agtgatgaac
     3001 ccggatctgc ttatttgtca gatggaaccc agtgttacat tggagattga attgaccatc
     3061 acaaaaggcc ggggatatgt cgccgcggac gaacaaccag agcgtgaaag tgtcataggt
     3121 gtaattcctg tggattctat ttatactccg gttaagaatg tcgcttacca ggtttccaat
     3181 acaagggtag gacaacgtac agactatgag aagttgacca tcgaggtgaa gacagatggg
     3241 acaatacatc cggaggatgc agtgaaggag gcttcaagga ttttgatcca gcatctgatg
     3301 ctcatcactg atgaaaatat ttcatttgat gatgctgcta aaggtgagga agatgttgtc
     3361 gatgaacata tcctgcacat gcgtaagttg ttgaagactt ctctggagga tctggatctg
     3421 tcggtaagag cttacaactg tctgaaagct gccaaaatca attccctggg tgaaatggta
     3481 caatatgaaa ctcatgaatt actgaagttc cgcaattttg gtaagaagtc tttggtcgag
     3541 atcgaagaat tactttctac aaaaggactg cactttggta tggacctttc caaatacaag
     3601 ctggaagaag aataatttaa tctataaaat tgcaaattac cggtgcctat tgcccggtgt
     3661 tgcgaagcta aacatttgaa aaaatgagac acggtaacaa agtcaatgcc cttgggcgta
     3721 ctaaatcaca tcgggatgca gtaatgaaaa acatggcatc ttccctgatc gagcata
//
LOCUS       DAOQMC010000226         3776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12464_length_3776_cov_5.198603, whole genome shotgun sequence.
ACCESSION   DAOQMC010000226 DAOQMC010000000
VERSION     DAOQMC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12464_length_3776_cov_5.198603"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKV31_08510"
     CDS             complement(<1..364)
                     /locus_tag="PKV31_08510"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92940.1"
                     /translation="MVFLQIAKELIRDTDKEVVSVVYWMDKFSELVIGFIPRLILALL
                     LYILGTWLIRKALRILTKIFHRSHIDPSLRNFLIMLTRIILVILLLLTVISLLGINIT
                     SFAALLAGLPITVAGCTIN"
     gene            622..2808
                     /locus_tag="PKV31_08515"
     CDS             622..2808
                     /locus_tag="PKV31_08515"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNB92941.1"
                     /translation="MFRFFSAILIFFLCTACGLSGFAQKKDLPVNFVNPLIGTAGHGH
                     VFLGANVPFGMVQVGPNNIGKGWDWCSGYHYSSKEILGFSHLHLSGTGIGDWGDILVL
                     PATDKPKTIAARKDDLSDGYGSPFDHSNEECKPGYYKIYLDRYKVTAEISATSRAAIH
                     HYRYDAGVNNPHILLDLSFGIGWDGPKDMDLRRLDDSTYIGHRLSKGWADDQRVFFAI
                     RFRNKLGKIEIFNDTSKNNMAHAAAFHPHLDTDGTVDFKVAISSVSTENALLNLVTEM
                     PHWNFNQYREQATQLWNEALSVVDYKADHKKKTIFYTALYHSFFFPSVYDDVNGEYRS
                     SDQMVRQGVTNTNYTVFSLWDTYRAQHPMLTILKPRMVDDLINTMLRIYQEQGKLPVW
                     HLSGNETNTMVGYSAVPVVVDAWRKGFRGFDSNLAYEAVRHSSLQGTDGIQYIRKLQY
                     IPADSVHEAVAKALEYAISDGCIALMAEGLGHEGDASYYNKRSKMYSEYYDPETRFMR
                     GKMADGTWRPGFSPFEAKHRENDYCEGNAWQYTWLAPQDVNGLIELLGGAARFEAKLD
                     SLFVVTGDMGENASVDISGLIGQYAHGNEPSHHIPYLYNYIDRQDKTATLVHRIMHEM
                     YTDKPDGLCGNEDVGQMSAWYILSAMGFYPVHPAEGIFQIGTPMMNEARIKLPDNKTF
                     IIKVKRKKAEDYLVKSMKLNGQPYTKNYIHYKEIMAGGILEFILGK"
     gene            3064..3735
                     /locus_tag="PKV31_08520"
     CDS             3064..3735
                     /locus_tag="PKV31_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNB92942.1"
                     /translation="MAVKMRLQRKGRKQAPFYHIVVADSRSPRDGKYIERLGSYNPMT
                     KPATIDIDRELAFSWLMKGAEPTDTVRAILRFKGVLVKKHLMRGVAKGAFTQEEADKR
                     WQAWIDAKETRISERREGAKAEKVAKHKSIHGEAKVKPAPVVEEVPVEEAPVVEETTA
                     TEEVPAVVETPVAEEAPAAETPATEESPATEEAPVAEASVEEIPAEETPAEENKEAPA
                     DDQNA"
BASE COUNT         1182 a          763 c          856 g          975 t
ORIGIN      
        1 gattgattgt gcagccagct actgttattg gcaggcctgc aagcaaagcg gcaaaactgg
       61 tgatattgat gccaagcaga ctgatgacgg tcaaaagtaa caggattaca agtatgatcc
      121 tggtcaacat aatcaggaag ttcctgaggg aaggatcgat atggctgcgg tgaaaaattt
      181 tcgtcaaaat tctgagagcc tttcgaatca gccatgtccc caaaatatac aacagcaatg
      241 ctaagatcag cctgggaata aatcctataa ccagttccga aaacttatcc atccaataaa
      301 ctacactaac cacctcctta tcagtatctc gaatcaactc ttttgcaatt tgcagaaaaa
      361 ccattttggt aataatttca ctctaaaaaa acaaaaatac tttgaacttt gtatttaaaa
      421 ccacgaatat attcaattct aaatgtaacg aaacaatagt tttctaacca aaatctcctc
      481 cgggttgtat caagaataat ccgggacaaa atatgcccca taattagtct cagcctgttt
      541 aaattttaac caaccgggct gcaatcccgt tgaaatgcct taatattgca ataattattt
      601 tcattaaatt ttatgaatcg aatgtttcgc tttttctcag ccatattaat tttcttcctc
      661 tgtacagctt gtggtctgtc gggatttgca caaaaaaaag atttaccggt gaattttgtt
      721 aacccgctta tcggtacagc cggccatggt catgtattcc tgggggcaaa tgtacctttc
      781 ggtatggttc aggttgggcc aaacaatatt ggcaaaggct gggactggtg tagcggatat
      841 cattattcga gcaaggaaat tctcggtttt tcacatctgc atcttagtgg tacaggaatt
      901 ggcgattggg gagacatact ggtcttacct gcaaccgata agccgaaaac tattgcagcc
      961 aggaaggacg atctaagcga tggttacggt tctcccttcg accacagcaa tgaggaatgc
     1021 aagcccggat attataaaat ctatctggat cgttataagg taaccgccga aatatcagcc
     1081 acatcaagag ctgcaatcca tcattacaga tatgatgcag gggttaataa cccacatatt
     1141 ttacttgacc tatcattcgg aatcggctgg gatggcccta aggacatgga tctcaggcga
     1201 ttggacgact ccacatatat tggccaccga ctatcaaagg ggtgggctga tgaccagcgt
     1261 gtattctttg ccatccgctt cagaaataaa ttaggcaaaa tcgaaatttt caacgacact
     1321 tcaaagaaca acatggcgca tgctgccgcc tttcatcctc atcttgatac agatggaacc
     1381 gttgatttca aggttgcgat ttcgtctgtc agtaccgaaa atgctttgct caatttagtg
     1441 acagaaatgc cacattggaa cttcaaccaa tacagggaac aggcgacgca actatggaac
     1501 gaggctttgt cggtcgtgga ttacaaagca gatcacaaga aaaaaacaat attctacaca
     1561 gccttgtacc atagcttctt cttcccatca gtgtacgatg atgtcaacgg cgaatacaga
     1621 agtagtgacc aaatggtcag gcaaggtgtt acaaatacta attatacggt attttcgctt
     1681 tgggatactt atcgggctca acacccgatg ctaaccatac tcaaaccccg gatggtagac
     1741 gatctgatca atacaatgct tcgcatctat caggaacagg gcaaacttcc ggtatggcat
     1801 ctgtccggca acgagacgaa taccatggta ggctatagtg ctgtgccggt tgtcgtcgat
     1861 gcatggagga aaggttttcg gggatttgac tcaaatcttg cctatgaggc tgtccgacat
     1921 tcctcacttc aaggcacaga tgggattcaa tacattagaa aattacaata tatccctgcg
     1981 gactctgtac acgaagctgt tgcaaaggct cttgaatatg ccatcagcga tggttgcata
     2041 gctttgatgg cagaaggact tggccatgaa ggggatgctt cttattacaa taagcgaagc
     2101 aagatgtatt cggaatatta cgatcctgaa acaagattca tgcggggcaa aatggctgat
     2161 ggtacctgga gaccgggctt cagccccttt gaggcaaaac atcgggagaa tgattattgc
     2221 gaaggtaatg catggcaata tacctggctg gcacctcagg atgtaaatgg attaatagaa
     2281 ttgctggggg gggcagccag gtttgaagct aaacttgact cgctttttgt cgtgaccggg
     2341 gatatgggtg aaaatgccag tgtggatatt tcaggcctga taggtcagta tgcccatggc
     2401 aatgaaccaa gtcatcacat accctaccta tataattata tcgatcgtca ggataaaact
     2461 gccacactgg tgcatcgtat catgcatgaa atgtacaccg acaaacctga cgggctttgt
     2521 ggtaacgaag atgtaggtca gatgagtgcc tggtatatac tgtcggccat gggattctat
     2581 ccggtacatc cggcagaagg tatatttcag attggtacac cgatgatgaa tgaagctcgc
     2641 atcaaattgc cggacaataa gacctttatc atcaaagtaa aaagaaaaaa ggctgaagat
     2701 tatctggtca aatcaatgaa actaaatggc cagccttata ccaagaatta tatccactat
     2761 aaggaaatca tggcaggtgg tatactggaa ttcattttgg gtaaataatt taaaggaatg
     2821 cttgaaatta ttattatttc acgcgaaaat gtatttaaat tattccggtt ataaagattc
     2881 ggtggcaggg gcacaatcag gtgttggagt aatttttact atataaagga aaaatattaa
     2941 tccggcccat actctgaaat aaaaaaatat tcttatcttt gcgcctcgtt aaatccctgt
     3001 ggtttgctcg acaaaggaca tggggttgat attcatatat ttataacaaa aaatttaaca
     3061 gacatggcag ttaaaatgag attgcaaaga aaaggaagaa aacaagctcc tttttaccac
     3121 attgttgttg ctgattcccg ctctccacga gacggaaaat acatcgaaag actgggttca
     3181 tacaatccaa tgaccaaacc ggctactatc gatatcgata gagaattggc tttttcatgg
     3241 cttatgaagg gagctgagcc gacagataca gtcagagcta tcctgagatt caagggtgta
     3301 ttggtgaaga aacatcttat gagaggtgta gcaaaaggtg catttaccca ggaagaagcc
     3361 gacaagagat ggcaggcctg gatcgatgcc aaggagacac gaatctctga gagacgtgaa
     3421 ggagcgaagg ctgaaaaagt tgcaaagcat aagtcaattc atggcgaagc aaaggtcaaa
     3481 ccagctccgg tagttgagga ggtacctgtt gaagaagctc cggtagttga agaaaccaca
     3541 gcaacggaag aggttccggc tgtagtagag acacctgtag ctgaagaggc accggcggct
     3601 gaaacccctg ccaccgaaga atcgcccgca actgaggaag cgccggtagc cgaggcttcg
     3661 gttgaagaga ttccggcaga agagactccg gctgaggaaa acaaagaggc tcctgccgat
     3721 gatcaaaatg cgtgattaat atttttttaa tcaatagaga aatatttcac gaaaat
//
LOCUS       DAOQMC010000227         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12641_length_3744_cov_4.014096, whole genome shotgun sequence.
ACCESSION   DAOQMC010000227 DAOQMC010000000
VERSION     DAOQMC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12641_length_3744_cov_4.014096"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKV31_08525"
     CDS             <1..652
                     /locus_tag="PKV31_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNB92943.1"
                     /translation="SYCLDAEKMFIWKDVEGVLTADPRMFKNVQKLSWLSYKEAIEMT
                     YYGASVIHPKTIKPLQNKNIPLYVRSFINPEAEGTYIGPDVEEHYPPIVILDGNQTLL
                     HIATKDFSFVVEHHMARLFKVFADLRIFINMMRNTAISFTVCVPNIPDRIAKLKEILE
                     DEYTVTEEVNLELLTIRHYEEEVLKSLTRGKIVLMEERLRNTVQLVIKDIPLIERI"
     gene            729..802
                     /locus_tag="PKV31_08530"
     tRNA            729..802
                     /locus_tag="PKV31_08530"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:763..765,aa:Ala,seq:ggc)
     gene            complement(885..1514)
                     /locus_tag="PKV31_08535"
     CDS             complement(885..1514)
                     /locus_tag="PKV31_08535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB92944.1"
                     /translation="MIWVFILLIIALSYFIFAIFSIRHGIFIKSISRSPFGHGILTFD
                     DGPHPEFTPKILALLEQYHQKAIFFCIGKNANEHPEIIKQIAAAGHILGNHTMNHSIR
                     STFSNRTAYLKEINDTTHLLQSISGQEIKYFRPPFGVTNPEIAAAIKKSGLISMAWNI
                     RTFDTVSTDSAKICSKMIQNWNDNAIVLMHDNNVVTEEALNLFLGQISK"
     gene            complement(1516..2397)
                     /locus_tag="PKV31_08540"
     CDS             complement(1516..2397)
                     /locus_tag="PKV31_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNB92945.1"
                     /translation="MKAYINHINAISRYQYLEGSIIEQSEIIEPDYESILDVRAMRRL
                     SKILKMGLFTALSVKNQANRQPDGIICGTALGCLADTYQFLKNMSDRDEQLLTPTAFI
                     QSTHNTLSGTLGIMLQNHSYNITWSNREFSFYDALEDAIIRVNKCTDDHILLTAADEM
                     PRQVAEIIDSIPLDCPVPINSEGAASFWISSRPAGISITIESILTVQTSDVIKIVAET
                     SEKTIVINSNPFLSFTQTDRVHNLVNAHRSLIDDAFCLHVGCQLLSLCREKSNPKQEV
                     LCIGGNDAFSSLVKISC"
     gene            complement(2401..3591)
                     /locus_tag="PKV31_08545"
     CDS             complement(2401..3591)
                     /locus_tag="PKV31_08545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNB92946.1"
                     /translation="MPESRVVVTGMGIMAPLGRGVSANHAALVDNRHGLGDITILNTI
                     HRGSLPAGEIKLTNTDLASIANFQGENIPRSVLLSAVAIQEALASAGLHHNHDINFYY
                     STTVGGMDISEELNDKRLKGQDIDFSGFKYHDCGASTMLLGDFFGFTGRRYSLSTACS
                     SSANCIGRAMMDIKTGRARYAVAGGADALSRFTLNGFNSLMILDKELCNPMDQNRRGL
                     NLGEAAAFLVLESEENAVNRGAEILAIVSGFGNTNDSHHQTASSESGIGAVLAIREAL
                     NDAGLKAENISYINAHGTGTQNNDLSEGNALETVFGKVPPTASTKSYTGHTLAASGAV
                     EAIYSVLAIKNQEMYPTLRFKEAIVGHGFKSFDFTIQGEVNHVLSNAFGFGGNCSALI
                     FSKY"
BASE COUNT         1042 a          790 c          792 g         1120 t
ORIGIN      
        1 atcctattgc ctcgatgccg agaagatgtt tatttggaag gatgtagaag gtgtccttac
       61 agctgaccca cgcatgttta aaaatgtcca aaagttatcg tggctgtcgt ataaggaagc
      121 catagaaatg acctactacg gggcatcagt catccatcct aaaaccatca aaccactcca
      181 aaataaaaat attcctttgt acgtccggtc ttttatcaac cctgaagcgg aaggtactta
      241 tattggtccc gatgtggaag agcattatcc acctattgtt attctggatg gaaatcagac
      301 cttgcttcat attgctacta aggatttttc atttgttgtg gagcatcata tggctagatt
      361 gttcaaggtg tttgccgatt tgaggatctt tatcaatatg atgcgtaata ctgcaatatc
      421 ctttaccgtt tgtgttccga atattcctga ccggatagca aaacttaaag aaatactgga
      481 ggatgaatat accgtaacag aagaagtgaa tttagaattg cttactatcc gccattatga
      541 agaagaagtg ttaaagagtc tgacaagagg taagattgta ctaatggaag agaggctgcg
      601 gaatacagta caattggtaa taaaagacat tccactgatt gaaagaatct gattttttga
      661 aaaataaccc tgaaaaattt tctatttcac aagagggttt tatttttgtc actctctaag
      721 gctaaaaagg gggattagct cagttggcta gagcgcttgc ctggcaggca agaggtcacc
      781 ggttcgagcc cggtattctc cacttttttt acctccttcc aaacaccttt ttcctttttt
      841 aaccttattg tatgaattgt tagtattgct tcggatgagg tatatcactt agatatttgc
      901 cccaggaata aattcagcgc ctcctcagtg accacattat tgtcatgcat taatactatg
      961 gcgttatcat tccagttttg aatcattttg ctacagattt tggcagaatc agtgctgacg
     1021 gtatcaaagg ttcttatgtt ccaggccatg gaaatcaaac cggatttttt aatagctgct
     1081 gcaatttccg gattagtaac cccaaatggg ggtctgaaat atttgatttc ctggccggaa
     1141 atgctttgga gcaagtgggt ggtgtcattg atctctttca gatatgcagt ccgattgcta
     1201 aaagtggatc ggatactgtg gttcattgta tgattaccca agatgtggcc ggctgctgct
     1261 atttgtttta taatttcagg atgttcgttt gcattttttc cgatacagaa aaagatagct
     1321 ttttgatggt attgctccaa tagggcaagg attttaggcg taaactcagg atgaggtccg
     1381 tcgtcaaagg tcaatatacc atgcccaaag ggactcctgg agattgattt gatgaagatt
     1441 ccatggcgta tgctgaaaat cgcaaagatg aagtagctca atgctataat cagtaaaatg
     1501 aaaacccata tcattctagc aggaaatttt caccaaagaa gagaatgcat cattaccccc
     1561 aatacaaagc acttcttgtt tcggattgct cttttcgcga cacaaactca ataactgaca
     1621 tccgacatgt aagcaaaatg catcgtctat cagacttctg tgtgcattta ccagattatg
     1681 cacccgatcc gtttgagtga aactcaggaa tggatttgaa ttgataacga tggttttctc
     1741 ggatgtttca gctacgattt ttattacatc ggaagtttgg accgtcagaa tagattcaat
     1801 agtaattgaa atcccggcag gcctggaact aatccaaaag gaagcagcac cttcactatt
     1861 gattgggacc ggacaatcaa ggggtatgct gtctataatc tcggccacct gccgcggcat
     1921 ttcgtcagcc gccgtaagta gtatatggtc gtcagtacac ttgttgactc ttataatagc
     1981 atcttcaagt gcgtcgtaga atgaaaattc ccgattactc caggttatat tgtagctatg
     2041 attttgcagc attattccta aagtaccgga aagtgtgtta tgtgtggact gtatgaatgc
     2101 agtcggagta agaagctgtt catcgcggtc actcatattt ttcaaaaact ggtaagtatc
     2161 agccagacaa cccaaagcgg taccgcagat gattccatct ggttgcctgt tagcttgatt
     2221 ctttacagaa agtgcagtaa aaagtcccat cttcaggatt ttactcagcc tgcgcatagc
     2281 acgtacgtcc agtattgatt catagtcagg ttcgataatc tcgctttgct cgataattga
     2341 accttcgagg tattggtatc tgctgatggc attgatgtga ttgatgtaag ctttcattga
     2401 ttaatattta ctgaagatca aagcagaaca attcccgcca aaaccgaaag cattggataa
     2461 gacgtggtta acctctccct gtatggtaaa atcgaatgac ttaaagccgt gaccaacgat
     2521 cgcttcctta aatcttagtg ttggatacat ctcctggttt ttgatggcta aaacagaata
     2581 gatagcctct acagcgcctg aagcagccaa agtatgcccg gtataagact tggtagatgc
     2641 tgtggggggg accttaccaa aaacggtttc caatgcattt ccttccgaca gatcattatt
     2701 ttgggtgcct gttccgtgtg cgttgatgta ggaaatattt tcagctttca acccggcatc
     2761 gttcagcgct tcccgaattg caaggacagc cccaattcct gattcgctgc tggcagtttg
     2821 gtgatgtgag tcattggtat tgccaaaccc acttacgatg gccagtattt ctgcacctct
     2881 gtttacagcg ttttcttccg attccagtac cagaaaggcc gctgcttctc ccaaattgag
     2941 accacgcctg ttttgatcca tcggattgca caattcctta tccaatatca tcagggagtt
     3001 gaatccattc aaggtgaatc ggctaagcgc atctgctccg ccggctactg cataccgggc
     3061 acgacccgtc ttgatatcca tcattgctct gccaatacaa ttggctgatg atgaacatgc
     3121 tgtagaaagg ctgtaacgtc gacctgtaaa accaaaaaag tctcccaaaa gcattgtact
     3181 agccccacag tcatgatact taaagccact aaagtcaata tcttgacctt ttaacctttt
     3241 gtcgttcaat tcctctgata tatccatgcc accgacagta gtcgaatagt aaaaattgat
     3301 atcatgatta tgatgcaatc cggctgaagc taaggcttcc tgaattgcaa ccgcactcag
     3361 caacacagat cgagggatgt tctcgccctg gaagttggca atggatgcaa ggtctgtatt
     3421 tgtcaatttt atttcaccgg caggcaaact tcctctgtgg atagtgttca ggatagttat
     3481 atctcccaag ccatgacggt tatcgactaa agcagcatga ttggccgata cgccccggcc
     3541 caaaggtgcc ataatgccca ttccggttac tacaacacgg gattcaggca ttggcttggt
     3601 gcttatgaat atagttagcg attgtcctta cggatgtaaa tatttccctt gcttcctcct
     3661 gattcgtcaa tttgatgcca tagtcgttat caagcataac gatgatttcc agtgcatcta
     3721 ttgaatccag cccaatacga actc
//
LOCUS       DAOQMC010000228         3737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12678_length_3737_cov_5.156980, whole genome shotgun sequence.
ACCESSION   DAOQMC010000228 DAOQMC010000000
VERSION     DAOQMC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12678_length_3737_cov_5.156980"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..968)
                     /locus_tag="PKV31_08550"
     CDS             complement(225..968)
                     /locus_tag="PKV31_08550"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB92947.1"
                     /translation="MKAHNSVSGNKASWVFIEEILSMTEFCIEDIYSDMSEAMEKIAA
                     DDNGAVLVNPGREADFPESLTSYLQAIPKGSCHSVFVWDNDNRHVLRLKLKGDHPSDN
                     TDSTLDKKEINRDNEIPALPNAPIASEDGENTDTRLKPAVGATCTFFKSGTDFIKINF
                     QDILFFQSEHVYVNILLLGRKVPLRTKLDHVESFISDHGFCRIHQRYIVNISNIDRVT
                     SESVYIGNYELPLSKKFKKALLEKVGVFM"
     gene            complement(1365..1709)
                     /gene="rplT"
                     /locus_tag="PKV31_08555"
     CDS             complement(1365..1709)
                     /gene="rplT"
                     /locus_tag="PKV31_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNB92948.1"
                     /translation="MPRSVNAVASRQRRKKILKAAKGYFGARSKVLTVAKNAVDKANG
                     YAFRDRRNKKRAFRRLWIARINAASRTEGVSYSKLINLLREKNVGLNRKVLADLAMNH
                     TDTFKAVVNNVK"
     gene            complement(1803..2000)
                     /gene="rpmI"
                     /locus_tag="PKV31_08560"
     CDS             complement(1803..2000)
                     /gene="rpmI"
                     /locus_tag="PKV31_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNB92949.1"
                     /translation="MPKMKTHSSAKKRFRITGSGKVKRFQAYTSHMMRNKSKKAKLAL
                     RDSTLVSPADEKRVKALLGIQ"
     gene            complement(2222..>3737)
                     /locus_tag="PKV31_08565"
     CDS             complement(2222..>3737)
                     /locus_tag="PKV31_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNB92950.1"
                     /translation="TFAAQYWYLIIGFVLMMWLLWRGYRYTIRHTFRELSPLKFYLSS
                     SAVLFLSLGLGVLAFRGGLQTKPLNVIHASEMTSVQNIPAIINTPFSIFKSLRQKSMP
                     AMNFFPEEELSDLQGGIHRPATLDSFTRENVVIIIVESLSRKYIGYFGGEASTPFLDS
                     LFAVGYIFPNAFANAKESIQGIPAILSSIPSWQSEPFIFSYYSSNKITSMANLLDKVG
                     YTSSFYHGGYNGTMGFDSYAGLAGFDHYYGKNEYKNNDDYDGRWGIWDEPFLQYAADN
                     MDATSQPFFSAIFTLNPHHPFHIPGQYRKVFNKHRQPFLNCVEYEDYALRRFFERIRT
                     SPWFDNTLFVITADHTAINIEGEQSSIMEDYRIPIVFFRPKSGGLKGKSDIIASQIDI
                     LPSVMSLLHYPQPYYSLGTSLFEQTTKRYSINYNGNIYTYIDKDYCYQFNGENPVSLY
                     RWPEDTLCRNNLYSGIPDAILMARDTTLRKMLQQYSGDMTGNRMTYEAIRKSDK"
BASE COUNT         1089 a          805 c          760 g         1083 t
ORIGIN      
        1 ccgaatcgct tagctctcgg gacaagtata ccaaaaattt atggactatt atatatatca
       61 tcttggtata aaatatcact ctttaatcca tcagggaatt caatctaaac acatgcaacc
      121 tatgcgggtc gaatatttat attccgagaa acagattaag caacaccggc atatcagtag
      181 aaaattcaat ggaaagaaaa cacagcgcgg tcgaaaaagt accactacat aaacactcca
      241 accttctcca gcaacgcctt cttgaatttc ttggacaatg gcagttcata attgccgata
      301 tagacagatt cggaggtgac acggtcaata ttactgatat tgactatgta gcgctggtgg
      361 atccgacaaa aaccatgatc cgaaataaaa ctctcaacat gatcaagctt tgtgcgtagt
      421 ggaacttttc ttcccaacaa aagaatattc acatagacat gttcgctttg aaaaaagaga
      481 atatcttgaa aattaatctt aataaaatcg gttcctgatt tgaaaaaagt acacgtagca
      541 ccaacagcag gtttaagtcg ggtatccgta ttttcgccat cttcgcttgc aataggagca
      601 ttgggcaatg cgggtatttc attatcccga ttgatctcct tcttatctaa tgttgaatca
      661 gtattgtccg aagggtgatc acctttgagc ttcagacgta aaacatgccg attatcattg
      721 tcccatacga agacggaatg acaactgcct ttggggatgg cttgcaaata cgaagtcaag
      781 gattccggaa agtcagcctc ccgaccggga ttaaccagaa cggctccatt atcgtccgct
      841 gcaatctttt ccatagcttc agacatatcg ctataaatgt cttcaataca aaattccgtc
      901 atggatagaa tctcctcaat aaacacccac gatgctttat tgccggagac agaattgtgt
      961 gctttcatac taagactata aggttttata ttaaacttcc ccagggaatt gaaatcacat
     1021 aaaagcgaca atctgaaggt gcaattgtat attaaacaat taaatcgatt ttgaccgatt
     1081 taatacgctt ttttttgttt ttcagttcgc aaggtaataa atttttaatt taataatcaa
     1141 aaaaaagtca cttatatctc aaaattcttt tttctcaata tttttactcc tcgccaaacc
     1201 cttgcaggta acacatccgg cagaaaaaat taatgattgc ttcccctttt ctaaattgca
     1261 gattatagac taaggatata ccaaaacttc gcctattccc gactttatgc aataaaaaaa
     1321 gcctcgcaaa aatgcgaggc ttttatataa ataagtatat ttctttactt tacattgttg
     1381 acgacagcct tgaaggtatc tgtatggttc atcgcgaggt cggcaagaac ttttctgttg
     1441 agtcctacat tcttttctct cagcagattg atgagcttgg agtaggaaac accttctgtt
     1501 ctggaagctg cattaatcct ggctatccaa agccttctga atgcgcgctt tttgtttctc
     1561 ctgtcgcgga atgcatagcc attggctttg tcaacggcat tcttcgctac ggtaagaacc
     1621 ttagatcgtg cgccaaaata acctttggcc gctttaagaa ttttctttct tctttgtctg
     1681 gacgctactg cgtttactga acgtggcata ataatttggt tttagtacga tacagggctt
     1741 actcaaaata agctttggcc tgtatcctcg ttaaaaataa tttttaaatt ccgtggtgga
     1801 aattactgaa tacccagcaa agcctttacc cgcttttcat cggccggact gacaagggta
     1861 gaatcccgaa gggcaagttt cgccttcttg gacttattcc tcatcatgtg tgaggtataa
     1921 gcctgaaatc tttttacctt cccggagccc gtaatcctga atcgcttctt ggcactggaa
     1981 tgtgtcttca ttttaggcat aatatctatg cgtatttaat taagttaaac aaattagggt
     2041 gcaaaggtaa gcaattactt aatatcaaga cgctttccct cgaaaaaaat tgaaaattgt
     2101 tgtaattatt cagtttatct cctgtagaag tcttgcgctc aggccccttg gactatttga
     2161 tgtcatcacg taaagtggtt ggaccggaat aagtttcacg ttcaaattct aagatgtacg
     2221 cttatttatc cgactttcta atagcttcgt aagtcatccg gttacctgtc atatctccac
     2281 tgtattgctg tagcatcttc ctgagtgtag tgtcacgcgc catcaaaatt gcatccggta
     2341 taccggagta aagattgttc cggcaaagcg tatcttccgg ccagcggtaa agcgataccg
     2401 gattttcgcc attgaattga taacagtagt ccttatctat ataggtatat atgttgccat
     2461 tgtaattgat ggaatacctt tttgtggtct gctcaaatag gctggttccc aatgaatagt
     2521 aaggctgcgg gtaatgcagc aagctcatca cggaaggcaa gatatctatc tggctggcta
     2581 taatatcgct ttttcctttc agtccaccac tcttcggcct gaagaacaca atcggtatgc
     2641 ggtaatcttc catgatggag gattgttctc cttctatatt gattgcagtg tggtcagcag
     2701 ttatcacaaa cagggtattg tcaaaccacg gtgaagtcct gattctttca aaaaaccttc
     2761 taagtgcgta atcctcatat tccacacaat tgagaaaagg ctgacggtgc ttgttgaaca
     2821 ccttgcgata ctgcccggga atatgaaacg gatgatgcgg attgagggta aagatagccg
     2881 agaagaacgg ttgtgaggtg gcatccatgt tgtcggcggc atactgaagg aaaggttcat
     2941 cccagatacc ccatcgtcca tcatagtcat cattattttt atactcattc ttcccataat
     3001 agtgatcgaa acctgcaaga cccgcatagg agtcaaagcc catggtaccg ttgtaccccc
     3061 cgtggtagaa tgatgaggta tatcctactt tgtcgagcag attggccatt gaagtgatct
     3121 tgttggatga ataataggaa aaaatgaatg gctccgactg ccaggacggg atggaggaca
     3181 atatcgcggg gatgccctgg atagattcct tggcattggc gaaggcattg ggaaaaatat
     3241 aacccaccgc aaataatgag tcaaggaatg gtgtgcttgc ctcacctccg aaatacccga
     3301 tgtatttcct ggacaggctt tccacaatga taatgaccac attttcccgg gtaaatgaat
     3361 ccagggtggc gggccgatgt atccctccct gcaaatcaga gagttcctcc tccgggaaga
     3421 aattcatcgc aggcattgat ttctgcctga gtgacttaaa tatcgaaaat ggtgtgttga
     3481 tgatggcagg aatgttctgc acggaagtca tttctgaggc atggatcacg ttgagtggct
     3541 tagtctgcaa tccgccccgg aaagccagca cccccaaccc cagtgacaga aacagcaccg
     3601 cagaggagct cagatagaac tttaatggcg aaagctcccg gaaagtatgc cgaattgtat
     3661 accgataacc acgccacaac agccacatca tcagtacaaa tccgatgatc agataccaat
     3721 actgggcagc aaaggtt
//
LOCUS       DAOQMC010000229         3714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12810_length_3714_cov_4.819623, whole genome shotgun sequence.
ACCESSION   DAOQMC010000229 DAOQMC010000000
VERSION     DAOQMC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12810_length_3714_cov_4.819623"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1548
                     /locus_tag="PKV31_08570"
     CDS             127..1548
                     /locus_tag="PKV31_08570"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNB92951.1"
                     /translation="MRSLIWLLCFLVIGHDVADAQSVPWYALNGSDVRAARSLNPAFL
                     QNPHFESALGIGSISMDYNNNLGYFIDKSFMSLPIHFSEISVPELQFRKLGFNPALGI
                     DRELRGYEMKLNETDLARMYFSTSLSIAGPSYFRRLKSGMIVGITTGSSFIGGLKNYP
                     AHMTYGPYRRYSSGDRMPVPEFSGTADGRMYVGVNIAREFALLSGTRLGVGANIRYLG
                     GLMHFELSNDEAIREYFFRERDRVEVSNMRLDYKYSHSNANDLHAPWIRGNGMGGDLG
                     IYFESKLKSQQFSSIRGGIALNNLGFVRYSQGLRSGSFNIYEPTEISFGKFDTLIGFD
                     QLVDSFEYIVLKNPVNEHLQNRGGTIYTLPSLNMAFSFGLREYGKIHFFASSPLTANN
                     PEAFQIGVIPELTFNLVNIITPFSYNRWTGFRLGAGLNFGFLTFGTDDVRTFFKRERL
                     QSGSFYCAVHINYFSKSKNGKKK"
     gene            1852..2730
                     /gene="bamD"
                     /locus_tag="PKV31_08575"
     CDS             1852..2730
                     /gene="bamD"
                     /locus_tag="PKV31_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNB92952.1"
                     /translation="MKNKYLHYLCALMMRKHSLVLILLAMMAFASCKSEYEKLRASSD
                     VALVYKKALEYFSKKDYYKAQNLLEQILGRYRGKKEAEDLYVKYAYTHYYQGNYILAA
                     SYFQNFANTYLTSPLREEAAYMSAYSNYLQSPDYKLDQTYTGKAIDELQIFVNTFPKS
                     ERVKEANKLIDELRAKLEKKAFEEGMLYYNLKQYQSATITFNNMLKEFPDSKNVEKIR
                     YFIVKSNFTLAENSIYAKKLVRYQETLLSAELFKEKFPRSKDRKEIDSILKETKIHIK
                     KLENDGHQDKSAGNRS"
     gene            2690..3025
                     /locus_tag="PKV31_08580"
     CDS             2690..3025
                     /locus_tag="PKV31_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNB92953.1"
                     /translation="MTDIKTKVQGIDPNSHARNIRGMASKTGNIYEAICVISARARQL
                     QVGIKRELNSKLDEFTVATDTIEEIQENKEQIEISKFYEKLPNPAIIATEEFINGELS
                     FRYKEEHQD"
     gene            3068..>3714
                     /gene="coaBC"
                     /locus_tag="PKV31_08585"
     CDS             3068..>3714
                     /gene="coaBC"
                     /locus_tag="PKV31_08585"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNB92954.1"
                     /translation="MELQGKKIVLGICGSIAAYKAAFLCRLLIKKGAEVRVVMTPSAS
                     DFITPVTMSTLSNHSVYNSIHDGNTWAGHVDLGLWADLILIAPASANTIAHMANGICQ
                     TMLDAVCLSAKCTVCVAPAMDLDMWKHPATVANINRLRSFGTQIIPVEHGFLASGLIG
                     DGRMAEPEHILAYVIDYFNRGKDLLGQKVLITAGPTHEKIDPVRFIGNASSGKMG"
BASE COUNT         1132 a          680 c          819 g         1083 t
ORIGIN      
        1 tatgatattg aactgtatga atattagtgg ataatgcatt attgtaaaaa tatatgtggt
       61 tattaacaaa aagttgttaa actattgatt atttttgtta actattttta tattaggcta
      121 cttcttatga gatcattgat atggttgtta tgcttcctgg taatcgggca tgatgttgca
      181 gatgcgcagt cagttccatg gtatgcgttg aacggaagcg acgtgagggc tgccagatcc
      241 ctcaatccgg catttttaca gaatccccat ttcgagtctg cattagggat tgggtcgatc
      301 tctatggact acaacaataa tcttggatat ttcattgata aaagcttcat gagtcttccg
      361 atacatttta gtgaaatatc tgtgccggaa ttacagttca gaaaacttgg atttaatccg
      421 gctcttggta tcgacagaga actacgcggt tatgaaatga aactgaatga gactgatttg
      481 gccagaatgt acttttcgac ctctttgtcg atcgcaggcc cttcttattt caggagattg
      541 aaaagcggga tgatcgtagg tataacaacg gggtcttcat tcatcggagg attaaagaat
      601 tacccggcac atatgacgta tggtccatat agaagatatt cctccggaga tcggatgcct
      661 gtaccagagt tttcaggtac agccgatggt aggatgtatg tcggagtcaa tatagccagg
      721 gagtttgctt tgttaagtgg tacacgactt ggcgtgggcg cgaatattcg ttatttgggt
      781 gggctgatgc attttgaatt atcaaatgac gaagcgatta gagaatattt ttttagagag
      841 agggacaggg tagaagtatc caatatgcgg ttggattata aatattcaca ttcaaacgcg
      901 aatgatcttc atgcaccatg gatcagagga aatggaatgg gaggcgactt gggcatttat
      961 ttcgaaagta agctcaaatc ccagcaattc agttccatac gaggcggaat cgccctcaat
     1021 aaccttggtt ttgtaagata ttcccaaggg cttagaagcg gatcctttaa tatttatgag
     1081 cccactgaaa tctcctttgg aaaattcgat accttgatag gcttcgatca attagtagat
     1141 agttttgaat atattgtatt gaaaaatccg gtcaatgagc acctccagaa ccggggaggg
     1201 acaatctata cgctcccgtc tcttaatatg gcatttagtt ttggactacg cgaatatggg
     1261 aaaatacatt tcttcgcttc ttcaccctta acagccaaca atcctgaggc ctttcaaatc
     1321 ggagtgatac cggaactgac cttcaacctg gtaaatatca ttacaccttt ctcatataac
     1381 cggtggacag gattcaggtt gggcgcagga ctcaattttg gttttctgac atttggtacc
     1441 gacgatgtca gaactttttt caaacgagag cgattacagt cagggtcgtt ctattgtgcg
     1501 gttcatatca attatttttc taagtcaaaa aatggaaaga agaagtgagc atagatggtt
     1561 ttgcttggtg aattaaaagt atatccggtc tttccttccc ttgaggtttc cattcaggat
     1621 atcccgggca atatattaaa taaataatga gacttattcg tttttaagga ttcttaaaac
     1681 ctgcattgta aggatgttga ttgtatctgt catcatgaac ctatttttga aatagcatac
     1741 attctaatgg ccatttaagc aattttggca cattatgttt acttataggc atcagagccg
     1801 gaaatttttt cgttgatact aaggtacttt ctgaatcaaa taatttttac tttgaaaaac
     1861 aaatacttgc attatctttg cgcccttatg atgagaaagc attcacttgt cctgatttta
     1921 cttgctatga tggcctttgc atcctgtaag tcggagtatg aaaaacttcg ggccagcagt
     1981 gatgtagcat tggtttacaa aaaagcactg gaatattttt ccaagaagga ttattacaag
     2041 gcgcagaacc tgctggagca aatccttggt cgctatagag gaaaaaagga agctgaggac
     2101 ctgtatgtaa agtatgcata tacccattat taccagggga attatatcct ggcagcatct
     2161 tattttcaaa actttgccaa tacctatctg acaagccctc taagagagga agctgcctat
     2221 atgtcggctt actccaatta ccttcaaagt cctgactata agcttgatca gacatatacc
     2281 ggcaaggcga ttgatgaatt acagatcttc gtgaatactt ttccgaaaag cgagagagta
     2341 aaagaggcca ataaattgat agacgaactg cgtgccaagc ttgaaaagaa ggcgtttgag
     2401 gaaggtatgc tatactataa tctgaaacaa tatcaatcgg ccacgataac tttcaataac
     2461 atgttgaagg aatttcctga ttccaaaaac gttgaaaaaa tcaggtattt cattgtaaaa
     2521 tctaatttta ctcttgcgga aaacagtatt tacgcaaaaa agctggtacg atatcaggaa
     2581 actttattgt ccgctgaatt gtttaaggag aagtttccta gaagcaagga cagaaaagag
     2641 atagactcaa tactcaaaga aacaaaaatt catattaaaa aactggaaaa tgacggacat
     2701 caagacaaaa gtgcagggaa tcgatcctaa tagtcatgca cgaaatatca gagggatggc
     2761 ttcaaagaca ggaaatatct acgaagcgat ctgtgttatt tctgcaagag cacgtcaact
     2821 tcaggtcggc ataaagcggg aactaaacag caaactggat gaatttacag ttgcaactga
     2881 tacaatcgaa gaaattcagg agaataaaga gcagatagaa atatcaaagt tctatgagaa
     2941 gttgcctaat ccggcgataa ttgcgacaga agaatttatc aacggagaac tttcatttcg
     3001 gtataaagaa gagcatcagg attgatttcc cggataggcc ttgcctgatt gccaattcct
     3061 attaaaaatg gaactgcagg gtaaaaaaat agtattgggc atatgtggca gtatagcagc
     3121 ctataaggcg gctttcctgt gcagattgct tataaagaaa ggagcagaag tcagggttgt
     3181 gatgacccct tctgcttctg attttattac acctgttacc atgtccacgc ttagcaatca
     3241 ttccgtgtat aattccatcc atgatgggaa cacctgggcc gggcatgttg atttggggct
     3301 gtgggcggat ttgattctta ttgctccggc atcagccaac accatagccc atatggcaaa
     3361 cggtatttgt caaaccatgc ttgatgctgt ttgtcttagt gccaaatgta cggtatgcgt
     3421 agctccggcc atggatctcg atatgtggaa acaccccgcg acagttgcca atatcaatcg
     3481 attgcgaagc ttcggcaccc aaatcattcc ggtagaacat ggattccttg cgtcagggct
     3541 cattggtgat ggcaggatgg ccgagcctga gcatattttg gcatatgtca tcgattattt
     3601 caaccgggga aaggatctct taggccaaaa agtacttatt actgcaggcc cgacacacga
     3661 aaaaatcgac cctgtgagat tcattggcaa tgcttcaagc ggtaagatgg gtat
//
LOCUS       DAOQMC010000230         3706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12850_length_3706_cov_4.773213, whole genome shotgun sequence.
ACCESSION   DAOQMC010000230 DAOQMC010000000
VERSION     DAOQMC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12850_length_3706_cov_4.773213"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1563)
                     /locus_tag="PKV31_08590"
     CDS             complement(103..1563)
                     /locus_tag="PKV31_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNB92955.1"
                     /translation="MSNYLTRRKSLTSLLSDASESEKGLKRTLSSTSLVALGIGAIIG
                     AGLFSLTGIAAAENAGPAVTISFVLAGIGCAFAGLCYAEFASMIPVAGSAYTYSYATM
                     GEFMAWIIGWDLVLEYALGAATVGVSWSRYFLEFMSGLGIHFPASLVCGPFESVNLSN
                     GTVIPPGIINLPAVIIVSLLSLLLMRGTKGSSTINNILVVVKVAIVLMFIVLGWKFIN
                     PNNYTPYIPENTGEFGHFGWSGIARGAAVVFFAFIGFDAVSTAAQEAKNPQKGMPIGI
                     LGSLLVCTILYVLFAHVMTGLTPYTSFAGDAKPAATAFAVTGYTFLLNFLIIAILAGY
                     TSVILVMLLGQSRVFYSMSHDGLLPKFFSDIHPKFRTPWKTNLFFAAFVSVFAGFVPI
                     SDLGHMVSIGTLLAFVLVCFGVLYMRKKMPDAKRAFKTPFVPIVPIFGILVCLYLMTS
                     LPLESWFRLIAWLIIGLIIYFVYGKKNSKLGTADQG"
     gene            complement(1833..>3706)
                     /locus_tag="PKV31_08595"
     CDS             complement(1833..>3706)
                     /locus_tag="PKV31_08595"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNB92956.1"
                     /translation="TAGGAADDAYITDQFAASGKNSIKIKATSDNGGPEDIILKIGNN
                     MKAGTIDVSWNMLVAEGKVGYFNFQTTPNPGTGWAMEFFMNKDGSIDVVTNSKPFSNR
                     LSYNPGKWFNFRVVIDASNNLWQVYIDGECKGVFRNNLTTVSSIDFFAIANGEYYIDD
                     VVLNSDANDVKVYSGVEAGIMNLNITEGIQIAGTNIKTSVQLYNVGADTITSIEYSIK
                     NGGDVIEEKLEGLSLAPNKSQNIAIPTAIKLADGDNALILTLTKINGKEDELSCNNIL
                     NASAYAVQPAKNRKVLLEEGTGTWCGWCPRGAVFLSQIYPSYDGYLVPIAVHNGDPMT
                     VSTYDKEVTSFNGFTGFPGMIVDRRIVVDPSAALAPGLGYLREEPDATLLIGATEPEG
                     DGSMKVSVSLDYLNEVPKGYSVVLTIVEDQVKGSTAQYNQANYYSGGGSGQMGGYENL
                     PNPVPAAQMVYEHVARTHDHSVKNMDAYPAGEKAVLNYTVKLNSDWNQDEIKIVAFLL
                     NTANQVSNVDGVTLKDAYNNGFVSSTKEKILVNSGLTVYPNPVQGTTTIAINLDKPTD
                     VTTNILDAMGRIVLTKNYGKQNGHAVMNLDVNNLAPGSYTVLVRTTDGIATHKMVVQ"
BASE COUNT         1127 a          877 c          711 g          991 t
ORIGIN      
        1 taacgaatgt accttcggta aaagatcact tcaataaaag aacttctata aattaaaaag
       61 gcggagataa aactccgcct ataagtcatt aatttaaaag aatcaaccct ggtctgcagt
      121 ccccaacttg ctattctttt tgccataaac aaagtaaata atcagtccga taatcagcca
      181 ggcgatcaac ctaaaccagc tttccagtgg cagggaagtc atcaggtaaa ggcaaacaag
      241 gatgccaaat atcgggacta taggaacaaa tggagtcttg aaggctcttt tggcatctgg
      301 catttttttc ctcatataca aaacgccaaa gcataccagt acaaatgcca ataatgtccc
      361 aatgcttacc atatggccaa ggtccgaaat cggtacgaaa ccggcaaaga cagatacaaa
      421 ggccgcaaag aaaagattag tcttccatgg tgtgcggaat ttcgggtgga tatcgctgaa
      481 aaacttaggc aataaaccat cgtggctcat ggagtaaaac accctactct ggccaagcaa
      541 catgacgagg ataactgaag tatatcctgc cagaattgca atgatcagaa agttaagtaa
      601 aaaggtgtat ccggtaacag caaaagcagt agctgcaggt ttggcatcgc ccgcaaagga
      661 agtatatgga gtcaaaccgg tcatcacatg agcgaataaa acatataata ttgtacatac
      721 aagcagggaa ccaaggattc cgatcggcat acctttttgt ggattcttag cttcctgtgc
      781 tgccgtactg acggcatcga agccaataaa ggcaaagaat accactgctg cacctctggc
      841 gataccactc catccaaaat gaccaaactc accggtattt tcaggaatat agggtgtgta
      901 gttattagga ttgataaatt tccatccaag tacaataaac atcaacacaa tcgccacctt
      961 gacgacaacc aaaatattat tgattgtaga agatcccttt gtaccacgca tcagcaacaa
     1021 agacaacaag cttacaatga tcactgccgg caggttgatg atacccggag gaatgaccgt
     1081 gccattggaa aggttgactg attcgaaagg cccacaaacc agagacgccg ggaaatgaat
     1141 tcccaaacca ctcataaatt cgaggaaata tctcgaccag ctgactccca cggtagcggc
     1201 accaagggca tattcaagca caagatccca accaattatc cacgccataa attcgcccat
     1261 tgtcgcatag ctgtatgtat aagcgcttcc tgcaacaggg atcatactgg caaattcagc
     1321 ataacataat ccggcaaaag cacaccctat acctgctagg acaaaggata tggtcactgc
     1381 aggaccggca ttttctgctg ccgcaatacc ggtaagggaa aacaatcctg caccgatgat
     1441 ggcaccaata cccaacgcaa ccaatgaagt ggaggataag gttcttttta aacctttttc
     1501 gctttcactg gcatccgata ataaggaggt caatgatttc cgacgtgtta aataattcga
     1561 catttttcgt tatttatatt tttgatcggt aaatatatgg aaaacacaag tcgcatccat
     1621 attttttttg aaaaattaga cattattgca ataaaatatt gaaaatcaac cacggcacca
     1681 acaaaaaaat ttgtaccttc tgacaacgcc tatacgatac atgctttctc taatcataat
     1741 tcaaaaaaaa gagccatggc atcttaccat ggccctatca aaggatttcc tttcacccgt
     1801 caaggcatta ttgccctcaa cgaatgtata atttattgaa caaccatctt atgcgtagca
     1861 atgccatcag tggtacgtac cagcacagta taagaacccg gagcaagatt gttgacatca
     1921 agattcataa ctgcatgacc attctgcttg ccgtagttct tcgttaaaac aattcgtccc
     1981 attgcatcca ggatgttagt agtgacatca gtcggcttgt caaggttaat agcgattgta
     2041 gtagttcctt gtacaggatt cggatacacg gtcagaccac tgttgaccag aattttttcc
     2101 ttagttgatg acacgaagcc gttgttataa gcatctttga gtgtaactcc atctacattg
     2161 gaaacctggt tggcagtgtt aagcaggaag gcaacgattt taatctcatc ctgattccag
     2221 tcggaattga gctttacggt gtaattgaga acggcctttt ctcctgccgg ataggcatcc
     2281 atattcttca cagagtggtc gtgtgtacga gccacatgct cataaaccat ctgagctgca
     2341 ggaaccggat tgggaagatt ttcgtaaccg cccatctgac cgcttccacc accggaataa
     2401 taattggcct gattatattg tgcagtactc cctttgacct gatcctcaac gatggtaagg
     2461 acgacactgt agccttttgg cacctcattc aggtagtcca gacttaccga aactttcata
     2521 cttccgtcac cttcaggctc tgttgcacca atcagtagtg ttgcatccgg ttcttcccta
     2581 aggtaaccaa gtcccggtgc caaagcggca gaaggatcca ccacaatacg acgatcaact
     2641 atcatccctg ggaatccggt aaatccattg aaagaagtaa cctctttgtc ataggtgctt
     2701 accgtcatag gatcaccatt gtgaacagcg atcggaacca gatatccgtc atagcttgga
     2761 tatatctgtg acagaaatac agcacctctt gggcaccaac cacaccatgt acctgtccct
     2821 tcctctaaca gaacctttct gtttttggcc ggttggactg cataagctga cgcattaagt
     2881 atattattac atgacaactc atcctctttc ccattgattt tggtcaatgt aagtattaag
     2941 gcattatccc cgtctgccag cttgatcgct gtagggattg caatattctg agacttatta
     3001 ggagccagag aaagcccttc caacttctct tcgatcacat caccgccatt ctttatgcta
     3061 tattcaatgc ttgtgattgt atctgctcct acattatata attgaacact tgttttgata
     3121 ttggtgcctg cgatctggat accctctgtt atattcaggt tcataatacc tgcctctacc
     3181 ccactatata ccttcacgtc attagcatca gaattgagta ccacatcatc gatatagtat
     3241 tctccgttgg caatggcgaa aaagtcaata gatgagacag ttgtcagatt attcctgaaa
     3301 acacccttac attcaccatc gatatatacc tgccataaat tattggaagc atcaatcact
     3361 actctgaagt taaaccactt gcccggatta taactaagac ggttggaaaa tggcttggag
     3421 ttagtgacga catcaatact accgtcttta ttcataaaga attccattgc ccatcctgta
     3481 cccggattgg gagtggtctg gaaattgaaa taaccgacct taccttcagc caccagcata
     3541 ttccaggaga catcaattgt tcctgctttc atattatttc cgatcttcag gattatatcc
     3601 tccggacctc cattatcact ggtagcttta atcttaatac tgttcttacc gctcgcagca
     3661 aactggtcgg tgatgtatgc atcgtcagca gcaccacctg ctgtag
//
LOCUS       DAOQMC010000231         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_12872_length_3702_cov_4.926789, whole genome shotgun sequence.
ACCESSION   DAOQMC010000231 DAOQMC010000000
VERSION     DAOQMC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12872_length_3702_cov_4.926789"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="PKV31_08600"
     CDS             <1..1907
                     /locus_tag="PKV31_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92957.1"
                     /translation="QFGANHAFNGNTGSCSGNRNGSTAYEPGSGSTIMSYAGICGAQN
                     IQPHSDPYFHWVSMGEALTHINTTTSCRTAVSGTNNTTPQITSFTTGKTIPANTPFYL
                     DASATDADGDAITYCWEENDLGTAGNINASSTTAPIFRSFNPVSSGRRYFPRLHDVLN
                     NTTTYGEVLPSVARTLKFTITARDNHATGGGICRQSSTITVVASSGFKVTAPNVATTW
                     SSPGTGTITWDVAGTASSPISCANVDVQVSFDNGLSWTSIGVGVPNNGSFIWTIPSGI
                     NSSDVRFRVRCSDNIFYAVNTVPITIGNPVNTCYTYTNNSTLTIPLTPSTVTSSIIIS
                     NRAADEIVKDINITNLNVTHSNLRDLRFKLKSSNGASNILMDSKCSGYANINIGFDDE
                     AVSGTLPCPPTNGGAYKPQEFLNVHDGMPANGQWDLEIDDWYSGNGGSLNSWSVQICV
                     EKKTPLPLHLLSFAAEAVKIGVYIGWTTSNEREVEVFTVERMAHDGSFRSIGHIPAKN
                     IDAISNYGLVDEYPDKGINYYRLKMIGKNGKVSYSEIKSVRFEGRSSVTIFPNPTNGI
                     LSIKRIGNEGEIHSINILDLSGREVMSLPMRLHLYELKDIDVSSLIPGMYLVQVNMNT
                     GVETIPFIKS"
     gene            1942..2118
                     /locus_tag="PKV31_08605"
     CDS             1942..2118
                     /locus_tag="PKV31_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92958.1"
                     /translation="MDDDGSDRGKVQISLAEGLWTLTFLIEGGVHFDECCKKQLPGRM
                     YYPFIKGLQKRCYS"
     gene            2526..3665
                     /locus_tag="PKV31_08610"
     CDS             2526..3665
                     /locus_tag="PKV31_08610"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNB92959.1"
                     /translation="MIISVLRESKDGERRVALTPDVIKLLIKKGFQVKVESGAGSNSF
                     FSDDEYVRAGAVIASTRQEACSQDIILKVNAPSTEEVSMMKTGACCISLLYGYTQPVL
                     LDAFNARSITSFGMDAIPRISRAQKMDALSSQANLAGYKSVILGCDALSKIFPLLMTA
                     AGTITPSKVLIFGAGVAGLQAVATARRLGAIVEVTDVRPETKEQVESLGGRFLSVESD
                     AEVKTEGGYAREVSAEFLQKQQQLIEERIKEADLVITTALVIGKKSPILVTEKMVDSM
                     KKGSVIIDMAVEWGGNCELSEMNKTKVYKGVTIIGEANLPSMLPINASQLYATNISTL
                     LMHLASPEGLNFDREEEIAKGCLITIDGSTVHEFTNKILNASSNK"
BASE COUNT         1066 a          729 c          864 g         1043 t
ORIGIN      
        1 atcaattcgg agccaatcat gccttcaatg gtaataccgg tagttgtagt ggtaatcgta
       61 atggttcaac ggcttatgaa cccggtagtg gttctacgat tatgagttat gcaggtattt
      121 gtggagctca gaatattcag cctcattcag atccttactt tcactgggta tctatgggcg
      181 aggctttgac gcatatcaat acaacaactt catgccgaac tgctgtttcg ggtaccaata
      241 acacaacccc gcagattaca agttttacaa cggggaaaac cataccggcc aatactcctt
      301 tttatcttga cgcctcggcc acagatgccg atggggatgc gataacctac tgttgggaag
      361 aaaatgattt gggcactgcc ggtaatatta atgcttcatc tactactgca cccatcttcc
      421 gttcattcaa tcccgtcagc tcagggagaa ggtattttcc gcgactgcat gatgtattga
      481 acaataccac gacatatggc gaggtgttgc cttccgtggc ccggacactt aagtttacta
      541 tcacagcaag ggataatcat gctacaggcg gaggtatctg tcgccaaagc tcaaccatta
      601 cagtagttgc cagttcagga tttaaggtga ctgctcccaa tgtggctaca acctggagca
      661 gtccaggtac gggcaccatc acatgggatg tagcgggtac ggcatcctcg ccaattagct
      721 gtgccaatgt cgatgttcag gtttcttttg ataatggtct ctcctggacg tctatcggtg
      781 taggagtgcc caataatggc agttttatct ggaccatccc atcgggtatc aattctagtg
      841 atgtccgttt tcgggtaagg tgttcagata atattttcta tgcagtcaac acggtaccca
      901 tcaccattgg aaatcctgtc aatacctgtt atacctacac taataattct accttgacca
      961 taccccttac accgagtaca gtcacatcct caatcattat aagtaaccgc gccgctgacg
     1021 aaatcgtgaa ggatatcaat atcaccaatc tgaatgtaac tcactccaat cttcgggatc
     1081 ttcgcttcaa gctcaaatcg tccaatggtg cgtccaatat tcttatggac agtaaatgtt
     1141 cagggtacgc taatatcaat atagggtttg acgatgaggc tgtatccggt actttgcctt
     1201 gtcctccaac caatggaggt gcctacaagc ctcaggaatt cctgaatgtt catgacggga
     1261 tgccggccaa tggtcaatgg gatctggaga tagatgactg gtattcgggc aatgggggat
     1321 cactcaatag ttggtctgtc caaatatgtg ttgaaaaaaa gaccccactg ccgttgcatt
     1381 tgttgtcatt tgctgcagaa gctgttaaaa tcggcgttta cattggatgg actacgtcca
     1441 atgaaaggga ggtggaagta tttactgtgg aaaggatggc tcacgatgga agctttcggt
     1501 ctatcggtca tattcccgca aaaaatattg acgccatctc caattatggc ttagtggatg
     1561 agtatccgga caaaggaatc aattattacc gattgaagat gattggaaag aatggaaagg
     1621 taagttattc agagattaag agcgtaagat ttgagggaag gtcaagcgtg acaatatttc
     1681 ccaatccgac aaatgggata ctgtcaataa aaagaattgg aaacgaagga gagattcatt
     1741 ccataaacat acttgacctg tccggtcgtg aagtaatgtc acttcctatg cgccttcatc
     1801 tatatgaatt aaaagatatc gatgtgtcct ccctgattcc cggcatgtat ctggtgcagg
     1861 ttaatatgaa tacgggagtg gagaccatac cattcattaa atcttagatc ttaaccttgt
     1921 taagctgagc ataccatatg gatggatgat gatggctcag atagaggaaa ggtgcagatc
     1981 tcattggcag aggggctttg gacattaacc ttcctgattg aaggtggcgt ccattttgat
     2041 gaatgctgca agaagcaatt gcccggtcga atgtattatc cgtttatcaa ggggttgcag
     2101 aagagatgtt acagttgatg tgtttatatg caatagctga gataatattc atcaaggatc
     2161 gatagtagca tccggttatg ttatgaacaa tttgtctttg ggccaaggta aatggttgtt
     2221 ctcattcatt ttggcatgat gacttattga cctggaattt caataaggat atcattgaaa
     2281 agcaatggaa ttccggtaaa acaatttacc gaattaatag aaatccaatg gtacagtgat
     2341 ttaccaatac aaaagtgcag gctctcgttt tggagtttat aataaatcat ttagtcggaa
     2401 ctcccactgg atggggtata ttatatcttc aatgagataa tttccaaaaa tatcttttga
     2461 tttttgattc ttttaacaga tatttatata tttattgctt acaaggtaaa ataatcggca
     2521 attatatgat aatatctgta ctcagagaat caaaggacgg agaaagaagg gtcgcattga
     2581 cacctgatgt gatcaaatta ctgataaaaa agggttttca agtcaaagta gaatccggtg
     2641 ccgggtcgaa ttcgtttttt tccgatgatg aatatgtccg cgcaggagcc gtaatcgcat
     2701 ctaccaggca agaagcttgt agtcaggaca tcattctaaa ggtcaatgct ccctccactg
     2761 aagaagtttc tatgatgaaa accggcgcat gttgtatttc cctcctttat ggatatactc
     2821 agcctgtgtt actggatgct ttcaatgcga gatccattac atcctttggt atggatgcaa
     2881 tcccgcggat ttcaagagct caaaaaatgg atgcgctcag ctctcaggca aatttagcag
     2941 gttataagtc ggtaatcctt ggatgtgatg ccttgagtaa gatttttcct ttattgatga
     3001 ctgcggcggg gactattacc ccatctaagg tcttgatctt cggggccggt gtggcggggc
     3061 tccaggcagt agccactgct cgaagacttg gagccatcgt cgaagtgacc gatgtgaggc
     3121 ctgaaaccaa ggagcaggta gagtctttgg ggggaagatt tcttagtgtc gagagtgatg
     3181 cggaggttaa gacagaaggc ggctatgcca gggaagtttc cgcggaattt cttcaaaagc
     3241 aacagcaact tatagaagaa agaataaagg aggcggatct ggttattacc acagcattag
     3301 tcatagggaa gaagtctccg atattggtga ctgaaaagat ggtcgattca atgaaaaaag
     3361 ggtctgtcat catcgatatg gcagtggagt ggggcggtaa ttgtgaatta tcagaaatga
     3421 ataaaaccaa ggtatacaag ggtgtcacaa tcattggtga ggccaacctg ccttccatgc
     3481 ttccaattaa tgccagtcaa ctctatgcga ccaatatcag cacacttttg atgcatctgg
     3541 cttcacccga agggttgaat ttcgatcggg aagaagagat agccaaaggt tgtctgataa
     3601 ccatcgatgg cagcactgtt catgaattta cgaataaaat cttaaacgct tcaagcaaca
     3661 aatagaataa tatgagtatc gatgtattac taccattgat gt
//
LOCUS       DAOQMC010000232         3658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_13121_length_3658_cov_5.187066, whole genome shotgun sequence.
ACCESSION   DAOQMC010000232 DAOQMC010000000
VERSION     DAOQMC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13121_length_3658_cov_5.187066"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="PKV31_08615"
     CDS             <1..1647
                     /locus_tag="PKV31_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92960.1"
                     /translation="VRMKDGNNRKLSGSGGIGLIASRLTLEGPIGSEHTSFIVSGRRT
                     YVDLITNLINKSKEDDPNWNKIPAYSFYDLNAKVNTRLGKNDRLYLSGYFGKDHFSLK
                     DSILDLGFSWGNATVTARWNHAFGNKLFANTSFIFSDYNYNISNQISGFNFSLGSKIR
                     NYAGKIDIDYNPNHRHAIRMGLSSTYYGFTVGRLKAGSDDGSISFSSGIERDGVEYGV
                     YVSDEFEVTNHLKANAGLRLSMWSSAGFFDAGLEPRLALAYEVDKRWSIKAGYARMKQ
                     YIHLVSNSGLSLPTDVWYPSTSVVHPQLSDQVSAGFSYLIVKGLLLTNEYYYKKMSNL
                     VDFRDGANLFLNNEIEQEFEFGKGYSYGTEISLEKESGKVTGWIAYTLAFVKRGAFAG
                     IMDGRYFAPRYDRRHDVKVVVSYKFNRRWTTSATWVYGSGDLAWLPGGRAVFQDTKGG
                     TFSPVTPIYGNRNTFRLPAYHRGDLSVIYTMSPRWGESNLTLSIYNVYDRRNPYFLTL
                     VTEYKDINQDGVTIKIPEKISARQVSLFPILPSLTWNFNF"
     gene            1851..2336
                     /locus_tag="PKV31_08620"
     CDS             1851..2336
                     /locus_tag="PKV31_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92961.1"
                     /translation="MHLQYKRSKLPDENRMLISVDIPDNLDILKKEAISMDDSNMYTA
                     MASIFQEKCEREDIDSESYIDDRWFDVRFISNNTQRRYDLFVSYIFVPENILNEQKLY
                     IAPKVLSKYPSTVYQQGSFQSQLIYLVSPDEKIGFKAVLINERQLEMDKLNRDGENYL
                     S"
     gene            2736..3593
                     /locus_tag="PKV31_08625"
     CDS             2736..3593
                     /locus_tag="PKV31_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNB92962.1"
                     /translation="MNKLIPCLALMLTLTSCNLSKEVDIDLPQYTSQPVVECYLIPGQ
                     RYELLLTHSNSFFDPLTVDNPTAYLASLLETGAMVRIIHGNDTVVLEEKVAFNSQTGF
                     ISNYSNEAIVPEDFNGEFSLSITLRNGSQIAANTHIPSPVGLDSIRAEYSNQEKTRAI
                     LFAYYRDNRSSVDYYRRLIHIASLDSVPMQNYVIDDKINTSENVAFGAFYERRDKRPV
                     VGDTLIFSLYHITEEYYRFSQSKDNANIANGNPFGQPGQIKSNVVGPSNPIGIFTGFV
                     PRRDTLILQ"
BASE COUNT         1097 a          692 c          836 g         1033 t
ORIGIN      
        1 gttcgcatga aagatggtaa caacaggaaa ttaagtggtt ccggcggcat cggcctgatc
       61 gcatcgagat tgacccttga gggcccaatc ggcagtgagc atacaagctt catagtgtca
      121 ggcaggcgta cttatgtgga tctgatcacg aatctcatca ataaaagtaa ggaggatgac
      181 cccaattgga ataagattcc ggcatatagc ttttacgatc ttaatgccaa ggtcaataca
      241 aggcttggta agaatgaccg gctctatcta agtggatatt tcggcaagga tcatttttcc
      301 ctgaaggata gtattcttga tcttgggttc agctggggca atgctactgt aacggctcgg
      361 tggaaccacg catttggaaa taaactattt gccaatactt cctttatatt ttccgattac
      421 aattataata tatccaatca gatcagtggt ttcaattttt ctttgggcag taagatccgc
      481 aattatgccg gaaaaattga catagattac aatcccaacc atcggcatgc tatcaggatg
      541 gggctttctt ctacttatta cggatttacc gttggcaggc ttaaggctgg ttctgatgac
      601 ggctctattt cattcagcag tggaatcgaa cgagatggcg tcgaatacgg tgtgtatgtg
      661 agcgatgagt ttgaggttac caatcacttg aaggccaatg caggattgcg attatccatg
      721 tggtcatccg ccggtttttt tgatgcaggg cttgagccgc gccttgcatt ggcctatgag
      781 gtagacaaaa gatggtccat caaggccggg tatgcccgaa tgaagcaata catccacctt
      841 gtatcaaatt ccggcttgtc gttaccgacc gatgtatggt acccttctac ctcggttgtt
      901 catcctcaac tttctgatca ggtgtcggcg ggctttagct atctgattgt caaaggtctt
      961 ctgctcacca atgaatatta ttataaaaag atgagcaatc tggtagattt tagggatggt
     1021 gcaaacttgt ttctcaacaa tgaaatagaa caggagtttg aatttggcaa agggtattcc
     1081 tatggcactg aaatttccct tgaaaaggag tcgggaaagg tgaccggctg gatagcttat
     1141 acactggcct ttgtcaagag aggtgcattt gcaggaatca tggatgggag atatttcgca
     1201 ccgagatatg acaggcgtca tgatgtcaag gtcgtggtct catataagtt taaccgacga
     1261 tggactacat cagcgacctg ggtctatggg tcaggtgacc tggcgtggtt gccgggagga
     1321 agagctgtgt ttcaggatac taaaggcggc accttcagcc cggtaacacc tatctatggt
     1381 aataggaata cctttagatt gcctgcttat caccgaggtg atctcagtgt gatttatacc
     1441 atgtccccac gctgggggga gagcaacctg acgctgagca tttacaacgt atatgaccgg
     1501 agaaatccat attttctcac attggtaacg gaatacaagg atataaatca ggatggtgtg
     1561 acgatcaaga ttcccgagaa aattagtgcc cgccaggttt cactttttcc aattctgcct
     1621 tccctgacct ggaattttaa tttttaagaa gtgaaagcag gacgatgaaa tcaagtatat
     1681 tctttcatgg taagatttga aaaggagaag gtgcaaaaga aggaatgttg tgttgggtga
     1741 atccggcaac ttatataata gatgaaccac gttatgatta tctcagaaaa tgaataaata
     1801 ttttgaggac ctacgaggca ttgatcaaac aggctaactt aatctaaaag atgcatttac
     1861 aatacaagcg aagtaaattg ccggatgaaa atcgaatgct gatatcggtg gatatacccg
     1921 acaatttgga tatcctaaaa aaggaggcaa tctcaatgga tgatagcaat atgtacacgg
     1981 ctatggcatc aatttttcaa gaaaaatgtg aaagggaaga tattgattcg gaaagttata
     2041 tcgacgacag gtggtttgat gtccgcttta tttccaacaa cacccaaaga agatatgact
     2101 tgttcgtttc ttatattttt gttcccgaaa atatcttgaa tgaacaaaaa ctatacattg
     2161 cccctaaggt gttgagcaaa tatccatcca ctgtttatca gcaaggttca ttccaatcac
     2221 aacttatcta tttggtttcg ccggatgaaa agattggttt taaggcagtt ttgatcaatg
     2281 aaaggcagtt agaaatggat aaattgaaca gagatggcga gaactacctg agctgagaac
     2341 taagtatgca ggatttttct ataaatacat taaggcggta tcgggtgtta gcaaatttac
     2401 ttttgttgga cgaaaggtat gacggcaacg aactatacct tgaaaaatgc tgcttttttt
     2461 gcaagaacgg agaaatagca tcgttggaat cagtggataa tgttggaatc agagaaatga
     2521 agacttatat cactcctaaa gtatgtttag tttggaatcc ggtgaatata acaattgaaa
     2581 agcgcttccg ttctcaatta atataccagc ttgtttgact tgaaaataaa tatcatgtat
     2641 tttattaaat gtaaaaagta cgattataat aatggtctaa cacaatcccc aacatcggcc
     2701 atatttcttc acaagatttt aaaaaaagta ggaaattgaa taaattaatc ccttgcctgg
     2761 cattgatgct gactctgact tcttgcaatc tcagtaaaga agttgacatc gatttgcctc
     2821 aatatacatc ccaaccggtg gtggaatgtt atttgattcc ggggcagcgc tatgaactac
     2881 tattgacaca tagcaatagt ttttttgatc cactgacagt ggacaatccc acagcctacc
     2941 ttgcttcctt gctggagaca ggggcaatgg tccgtattat acatggcaac gatacagttg
     3001 tactggaaga aaaggttgcg tttaatagtc aaaccggatt tatctccaat tattccaatg
     3061 aagctattgt gccggaagac tttaacggcg aattttcact cagcatcacc ctcaggaatg
     3121 gcagccaaat agccgctaat acacatatcc cttcacctgt aggccttgac agcattcgtg
     3181 ccgaatacag caatcaagag aagacgcggg ccatcttgtt tgcctattac agggataaca
     3241 gaagctccgt tgattattac agaagattaa tccatatagc tagcttagat agtgtaccga
     3301 tgcagaacta tgtcattgat gacaagatca acacgtcgga aaatgtcgct ttcggtgcct
     3361 tttatgaacg aagagataaa aggcctgtag tgggcgatac cctaattttt tcgttatatc
     3421 acatcacgga ggaatattat agattcagtc aaagtaagga taatgccaac attgccaatg
     3481 gcaatccttt tggtcagccc ggccagataa agtcgaatgt cgtcgggcca tccaatccga
     3541 ttgggatatt cacaggcttt gtaccacgtc gtgatacctt gatattgcag tgaggaactg
     3601 tggcaagaag aggtaaggtg tggactctcc gaagtaggta ggaaaataat agcgggcg
//
LOCUS       DAOQMC010000233         3640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_13241_length_3640_cov_4.976290, whole genome shotgun sequence.
ACCESSION   DAOQMC010000233 DAOQMC010000000
VERSION     DAOQMC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13241_length_3640_cov_4.976290"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /gene="lpxD"
                     /locus_tag="PKV31_08630"
     CDS             complement(<1..943)
                     /gene="lpxD"
                     /locus_tag="PKV31_08630"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNB92963.1"
                     /translation="MEITAGFITDFLGGTIIGDRNVVINGASTIEGGRPGTISFLSNL
                     KYEEYIYNSEASAILVDNSFEPSYPVKAALIKVENVYESLARLLDKFNVTTEIERHIS
                     PKAHIDDKAVLGNNVGVGVFSVIEENVRIGDNVEIENQVTIGQGVTIGQGTIIKSGVK
                     ILEGCVIGANVIIHSNTVIGSDGFGFAPTGEGNYVKIRQLGNVVIEDDVEIGSNCSID
                     RATFGSTIIRKGAKLDNLIQIAHNVEIGSNTVIAAQTGIAGSARIGEGCKIGGQVGIV
                     GHIQIADGTQIQAQSGVQSAVKNKNSKLFGSPALDYNN"
     gene            complement(1195..2148)
                     /locus_tag="PKV31_08635"
     CDS             complement(1195..2148)
                     /locus_tag="PKV31_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNB92964.1"
                     /translation="MIRYYAKQQGTLVELPDYAVHCWINIEPPFSHDELEDLSTRLNI
                     PLDFFTDPLDIDERSRYEKEGPARLIVMNSPILNETDRESESIYITIPIGIIFSPESV
                     ITICAHENPVLELFLEDKIKNFNPANIQHFILQILEQNVYRFISCLKKLNLKRNLLEQ
                     ELYDSSRNSELWQMLRIEKSLVYFVNSLKANELLKVKMKRIDFLGLGLNEELEEIYED
                     LMIDNSQALEMANIYTVITGSTMEAFASIISNNMNVDIQRLTFVTIMLMVPTIITGFY
                     GMNVKLPFENYKYASFFIIIFSVLVGAGLIYFLRQKRFRSS"
     gene            2327..2941
                     /locus_tag="PKV31_08640"
     CDS             2327..2941
                     /locus_tag="PKV31_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HNB92965.1"
                     /translation="MNMNENQVIEKQADSIIRNSIVFASGAGLIPVPVVDLMAVTAVQ
                     IEMIRRLSNLYKVDFKETEGKAAILALTSGGVARLGGRMIAKMIPVIGSIVGGIAASA
                     FSGAATYAVGEVFKRHFSEGGTFLDFDWKSFKHFYNEKFEKGKEYTAKIKKEEADNQP
                     QAQSEATNIYLDALQRLYDMKSTGAINEDDYEKLKADIMNKMGV"
     gene            complement(3237..>3640)
                     /locus_tag="PKV31_08645"
     CDS             complement(3237..>3640)
                     /locus_tag="PKV31_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB92966.1"
                     /translation="LYLKVKVTDARGCSTECYVEVDCSLDNPGINVVDPNVIDNSSTI
                     GQLNVINDYTLRPNPTSAQVYLDFESKLAEKVTINVTDNFGKLVYTKKVTTVKGFNTE
                     LLETNHLPVGLYQVSLISSDKLKTLQLIKVK"
BASE COUNT         1059 a          718 c          740 g         1123 t
ORIGIN      
        1 aattattata atcgagtgcc ggtgagccga acagcttact gttcttattc ttaacggcag
       61 attggacacc ggactgggcc tggatttgag ttccatcggc gatttgaata tgccctacaa
      121 ttcctacctg ccctccaatt ttacaacctt cacctattct ggcgcttcct gcaatgccgg
      181 tttgtgcagc gatgacagta ttactaccta tttctacatt gtgggcgatt tggataagat
      241 tgtccagctt ggctcctttg cggatgatgg tactgccaaa tgtcgccctg tctatactgc
      301 aattggatcc aatctcaaca tcatcttcaa tgacaacatt gccgagctgg cgtatcttga
      361 cataattccc ttccccggta ggcgcaaagc caaaaccgtc acttcctatc acagtattac
      421 tatgtatgat gacattagca ccaatcacgc acccttcgag aattttcact cctgacttga
      481 taattgtgcc ctgaccaatg gttacaccct ggccgatggt tacctgattt tcgatttcta
      541 cgttatcgcc aatgcgcaca ttttcttcaa taaccgaaaa aactccgaca ccaacattgt
      601 tgcccaaaac cgccttgtca tcgatatggg ctttcggact aatatgccgc tcgatttctg
      661 tagtgacatt gaatttgtca agcagtcttg ccagagattc atatacattc tccactttga
      721 tcagggctgc ttttacggga taggagggtt caaaggagtt gtcaaccaga atggcagaag
      781 cttcagaatt gtatatatat tcttcgtatt taaggttgga aagaaagctg atggttcccg
      841 gcctgcctcc ttcaattgta cttgctccat tgattacaac attcctatcc ccgatgatcg
      901 taccgcccaa aaagtcagtt ataaatcctg ccgtgatctc cattttcagc tatattttaa
      961 tattattttg caaagaaaca aagttttttg ataaatatgg attacgtttc agaaaacata
     1021 taaatattaa cgtatataat tgcctctttg gttggtaatt agctcaattt tcaaggatta
     1081 tttctacatg attaccgaat tgtatctgtt tgtctttttt attgtttgtt aagctaccta
     1141 aatagagtca atatccgggg cgcaagttat tgaaactgct tattcgaaat aaaattagga
     1201 ggatctgaat ctcttttggc gcaggaaata aattaaacct gctcctacta ataccgagaa
     1261 gatgatgatg aagaaggatg catatttata attttcaaaa gggagtttta cattcatacc
     1321 gtaaaatccg gtgatgatgg taggcaccat aagcatgatg gtaacgaaag tcaaacgctg
     1381 aatatcaacg ttcatgttgt tggatatgat gctggcgaag gcttccatag tacttccggt
     1441 gattacagtg tagatattgg ccatctcgag tgcctgactg ttgtcgatca tcaagtcctc
     1501 atagatctcc tccagctcct cattcaaacc caggcccaaa aaatcaatcc tcttcatctt
     1561 cactttaagc aattcgttgg ctttcagtga gttgacaaag tataccaaac ttttttcaat
     1621 tctgagcatt tgccataatt cactgtttct ggaagaatca tagagttcct gctccaacag
     1681 gttcctcttc aggttgagtt ttttaagaca tgaaatgaat ctatatacat tctgctcaag
     1741 aatctgcaat ataaaatgct gaatatttgc agggttgaaa ttcttgattt tatcctccaa
     1801 aaatagttcc agcaccgggt tttcatgtgc gcaaatggta atgacacttt cagggctgaa
     1861 gataataccg ataggaatcg taatgtaaat agactcgctt tccctgtctg tttcattcag
     1921 aatgggggaa ttcatcacta taagtcttgc aggcccttcc ttctcatatc gtgaacgctc
     1981 atcgatatca agcgggtcag tgaaaaagtc taatggtata ttgagcctgg tagaaagatc
     2041 ttccaactca tcgtgagaaa atggaggttc tatattgatc caacaatgta cggcataatc
     2101 cggcagttcg accaatgttc cttgctgctt tgcgtaatat cgaatcatgc gggtcgggat
     2161 ccatttacat tgttttggtt aaaaaatcaa attccggaca aaggtaaggc tattgttttg
     2221 tagaaacaaa aaaaagtctc attaatctgg tttttattgc cattggtcga gtaggggaac
     2281 aatccgaggt tggaattatg ttggttactt ataaaaactg gaataaatga atatgaatga
     2341 aaatcaggtt atagaaaaac aagcggatag cattattcgc aactctattg tttttgcatc
     2401 aggagccggg ctgatacctg tgccggttgt tgatttgatg gctgtcaccg cagtacagat
     2461 tgaaatgata cgaaggttgt ccaatctgta taaggtggat tttaaggaga cggaaggaaa
     2521 ggcagctatt ctggcattga cctccggtgg agtggctcga ttgggtggcc gaatgattgc
     2581 caagatgata ccggtgattg gaagtatcgt aggaggaatt gcggcttctg cattttcagg
     2641 agcagcaacc tatgctgtag gggaagtttt taaaaggcac ttttcagaag gaggaacttt
     2701 tttggatttt gactggaaaa gctttaagca cttttacaat gaaaaattcg agaaaggcaa
     2761 ggagtataca gccaaaatca aaaaagagga agccgataat caacctcagg ctcaaagcga
     2821 agcgaccaat atatatttgg atgccttaca aaggttgtat gatatgaagt caaccggagc
     2881 catcaatgaa gatgattacg aaaagctaaa ggcggacatc atgaataaaa tgggcgtgta
     2941 aatatagcgg ggcaattttc cgaatcttca ggtcaaccaa catgtgccgg ttttgggcga
     3001 tcccatgggg actgtaattt ggcctattca ataaatttag tcctataaat ttgtattgta
     3061 aatgattggt agactatcat attgagtcca cgaaaaccgg cttataatat atttctcaac
     3121 ttcattttgt actatttgga taaaaaaaag gggctgaatg ccccttttct atttcaaaat
     3181 gaaaaggaat cctcatgcga ggatccctct tccccatatt taactaaggc taaacgttat
     3241 ttaaccttaa ttaactgtaa tgtcttgagt ttatcagaag atatcagact cacctgatac
     3301 aaaccgaccg gtaggtggtt ggtttcaagg agttctgtat taaacccttt gacagtagtt
     3361 actttcttgg tgtacacaag tttaccgaag ttgtcagtaa cattgatggt cactttttca
     3421 gccaatttgg attcaaagtc aagatatacc tgagcacttg ttggatttgg ccttagagta
     3481 taatcgttga ttacattcag ctgtccaatt gttgagctgt tatcgattac attcgggtca
     3541 accacgttga ttccaggatt atcaaggcta caatcaactt ctacataaca ttcagtacta
     3601 catccacggg cgtccgtaac ctttactttc aaatacaatg
//
LOCUS       DAOQMC010000234         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_13723_length_3561_cov_5.280662, whole genome shotgun sequence.
ACCESSION   DAOQMC010000234 DAOQMC010000000
VERSION     DAOQMC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13723_length_3561_cov_5.280662"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKV31_08650"
     CDS             <1..544
                     /locus_tag="PKV31_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92967.1"
                     /translation="MYRIYKIVFKLMSGYVISFGNFVLIPRKQLSRLVYISEIWNHFP
                     GGIMRSKLPYISVPIDRGRRYKGRSKMGMVALVQHGISSITVNIDIVSVRLLLFSVGL
                     MLITFLLFCSLIVVKFFTNFAIPGWTSLIAISLLMIMLQSFFFSLLLTFVVLNARVQK
                     NFIPAIHHMDYVRSLKQYTF"
     gene            544..1254
                     /locus_tag="PKV31_08655"
     CDS             544..1254
                     /locus_tag="PKV31_08655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB92968.1"
                     /translation="MANIDYIGDELELFKHAVNWKDYYGKEIKPYISGEVCEVGAGIG
                     GTTNALLNRSVSSWLAVEPDGNLAEHIGAEVLGNPFASKVDVLHGTLDDIGEERQFDT
                     IIYIDVMEHIEGDQAEFDKAIGRLRKGGNLIILVPAFNFLYNDFDRRIGHFRRYNKGM
                     LKRLAEGRPLTRLKLSYYDSLGFFASVMNKLILKKPIPSAGNIKMWDNFMIPLSKLID
                     PLILRSFGKSLIAVWQKE"
     gene            1289..3400
                     /locus_tag="PKV31_08660"
     CDS             1289..3400
                     /locus_tag="PKV31_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNB92969.1"
                     /translation="MNTTSGAEAKWQYKDGLFKKIFFGVSLFLLIIMPILSFDFGITG
                     DELVHKQNGENVLKYLQTGGADTTYRSYKNLYLYGGLFDASAAMIYERMPNSDPYNVR
                     HVLNSLFGASLIIFTGLLARHLSRSWFVALIALLLTVLSPRLFGDSMNNPKDIPFAMA
                     YVMSVLGILRFNSLLPKWDWRSAIFLGVSMSIALNIRVGGLLLLVYFGLYTIVNVYLK
                     RKEFDSAKVNGLATIGKAAILGICSYFVGLIFFPYSHAAPITNTLEALKIMSNFDYAI
                     RMLFEGHALWSDEIPWYYIPKWLGMAIPLSVIIGLIMFLVRIRSIFKRLDWLPLAFIL
                     FVGIFPVVYAVAKHSSLYDGIRHFLFLMPMINVMAALGWANVMFAFWAKKFKWAVPAV
                     LGLLLVLPLRFMIANHPNEYIYFNELSGGIKKAYGNYETDYWMNSMKELSKWLISHDE
                     RIRKGEAVIVCTNSIDPVKHYFERWAPNVKVIYAAFKDRYKHKADYYLSIPRFIDSDL
                     LKNGSWPPKEVVHSVTVDGVPVGALSKYVDTFTYAGLNATKMQDYLLANEYFKKAYAN
                     DDKNNVAIANLALNYVNQDSLAAASQWADRGLALSPDNEDFLLPKGMILMYQGKVAEA
                     YEYFDRVKKMNKRNVLAYYYSAIIMENQKNYSAALDNLQKVLEQAPQFKQAYLLGAQI
                     MQNSGNPEAAARYMEAANNLK"
BASE COUNT          986 a          660 c          813 g         1102 t
ORIGIN      
        1 catgtatcgc atttacaaga ttgtattcaa actgatgagc ggctatgtga tttcctttgg
       61 caatttcgtc ctgattccca ggaagcaact ttcccgacta gtatatattt ctgaaatttg
      121 gaatcatttt ccgggtggca ttatgcgttc aaagttacct tatatcagtg tgccgattga
      181 tcggggacgt cggtataaag gcaggtcaaa gatgggtatg gttgccctgg tacaacatgg
      241 catcagcagt atcacagtca atattgacat cgtttctgtc agattgcttt tattttcagt
      301 cgggttgatg ctgattacat tcctgttatt ttgtagcctg attgtggtca agttctttac
      361 caattttgcc attccgggtt ggacgtccct gattgctatc agccttctta tgatcatgct
      421 gcaatccttt ttcttttcac ttttgctgac ttttgtggtg ttaaatgcca gagtacaaaa
      481 aaatttcatt cccgctatcc accatatgga ttatgttcgt tcccttaaac aatatacttt
      541 ctgatggcta atatcgatta tataggcgac gagctggaat tgttcaaaca cgcagtcaac
      601 tggaaagatt actatggaaa ggagattaaa ccttacatct ccggtgaagt atgtgaggtc
      661 ggagcaggca taggtggtac cacgaatgcc ctgttaaatc gttccgtttc ttcctggctt
      721 gctgtcgaac cggatgggaa tctggcagag catatcgggg cggaggtcct gggtaatcca
      781 ttcgcctcca aggtggatgt cctgcacggt acattggatg atatcggtga agaacgtcag
      841 tttgacacca ttatctatat tgatgtgatg gagcatatag agggagatca ggcagaattc
      901 gataaggcga tcggaagact gaggaaaggg ggaaatctga ttatcctggt accggccttt
      961 aattttcttt ataatgattt cgatagacga atcggtcatt ttaggaggta caacaaagga
     1021 atgttgaaaa gattggctga gggaagacca ctgacacgac tgaaactgag ttattacgat
     1081 agcttaggat ttttcgcctc tgtaatgaat aagttgatac tgaaaaagcc aattccttct
     1141 gccggaaata ttaaaatgtg ggataatttt atgattccat tgtcaaaact catagatccg
     1201 cttattttgc ggtcatttgg aaaatctttg atagcagtat ggcagaaaga gtgagtttaa
     1261 ttaaaaaaat gttttaagta taagaattat gaatacaaca agcggtgctg aagctaaatg
     1321 gcaatacaag gacggtcttt ttaagaaaat tttcttcgga gtatccttgt ttttgctaat
     1381 catcatgccg attctgagtt ttgattttgg aattaccgga gatgaactcg tccataagca
     1441 aaacggggag aatgtgttga aatatcttca aaccggtggg gcggatacga cataccgatc
     1501 ctacaaaaac ctctacctgt atggtggatt atttgatgca agtgcggcga tgatttacga
     1561 gcgtatgccg aattctgatc cttataatgt gcgtcatgtc ctcaactcct tattcggtgc
     1621 atcactaatt atctttaccg gtcttttggc acggcatttg tcccgaagct ggtttgtagc
     1681 tttgatagcc ttattattga ccgtgctctc accacgacta tttggcgaca gcatgaacaa
     1741 ccctaaggat atcccatttg cgatggcgta cgtcatgtcg gtattaggta ttttaaggtt
     1801 caactcttta ttgcctaaat gggattggcg ttcggcaata tttttgggag tttctatgtc
     1861 gatagccctc aatattaggg taggaggctt actgttacta gtgtattttg gtctttatac
     1921 aattgtaaat gtatatctga aacgtaaaga atttgacagc gccaaagtaa atggcctggc
     1981 aactataggt aaggcagcca tcttaggtat ctgttcctat ttcgttggat tgatattctt
     2041 tccttattcc catgctgcac ctatcaccaa tacattggag gctctgaaga tcatgtccaa
     2101 ttttgattat gccattagga tgctatttga aggccatgct ttatggtccg atgagattcc
     2161 ctggtattat atccctaaat ggctaggtat ggcaattccg ctttctgtca ttatcgggtt
     2221 gataatgttt ttggtcagga tacgttccat ctttaagaga ctggactggt tgccgctggc
     2281 tttcatcctt ttcgtcggaa tatttcctgt ggtctatgca gttgcaaaac acagttcctt
     2341 gtacgacgga atcaggcatt ttctctttct gatgccaatg atcaatgtga tggcagcatt
     2401 gggttgggcc aatgtgatgt ttgccttttg ggctaaaaaa tttaaatggg cggtgccggc
     2461 tgttctgggc ctcctgttag tacttccact tagatttatg attgccaatc accccaatga
     2521 atatatttat ttcaatgaat tatccggtgg tatcaagaaa gcctatggca actacgaaac
     2581 cgattattgg atgaacagta tgaaagaact ctctaagtgg ttgatctcac atgatgaaag
     2641 gatcaggaaa ggcgaggcgg tcattgtatg caccaatagt atagatcccg tcaagcatta
     2701 ttttgagcgc tgggcgccga atgtcaaggt aatctatgct gcttttaagg atcgttacaa
     2761 acataaagct gactattatc tatcaatccc gcggtttatc gatagtgatt tattaaagaa
     2821 cgggagttgg cccccgaaag aggtggtgca ttctgttacc gtagatggtg tcccggttgg
     2881 agcattgtcc aaatatgttg atacatttac ttatgcaggc ttaaatgcga ccaaaatgca
     2941 agattactta ttggcaaatg agtatttcaa gaaggcttat gccaatgacg acaagaataa
     3001 tgttgccatt gctaatttag ccttgaatta tgtcaatcag gattcactgg ctgcggcctc
     3061 ccaatgggct gataggggtc tcgcattatc gcctgataat gaagattttc tactgcctaa
     3121 aggtatgatt ctgatgtacc agggaaaagt agcagaagct tacgaatatt ttgatcgggt
     3181 caagaaaatg aacaaacgca atgttcttgc ctattactac agcgctataa ttatggaaaa
     3241 tcagaaaaat tactccgctg ctttggacaa tctgcaaaaa gttctggaac aggcacccca
     3301 atttaagcaa gcttatttat tgggagcaca gatcatgcaa aatagcggaa atccggaagc
     3361 agctgcaagg tatatggagg cagcaaacaa tttgaagtaa attctaatca aaggaaatgg
     3421 ataaaaaact tttcatactg agagagaagc aatccagggc attgagtggc ggtggttcac
     3481 atcggattga cagtcagcat gccaagggaa agcttacagc acgtgagaga atacaactgt
     3541 tgctcgatga agattctttt g
//
LOCUS       DAOQMC010000235         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_13805_length_3549_cov_3.882370, whole genome shotgun sequence.
ACCESSION   DAOQMC010000235 DAOQMC010000000
VERSION     DAOQMC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13805_length_3549_cov_3.882370"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKV31_08665"
     CDS             complement(<1..358)
                     /locus_tag="PKV31_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92970.1"
                     /translation="MKKLEEVAAICLGFEGNVEHISDNLTELYRIREIKYPKYFKMNL
                     LCKLGFIACDFLIEKHHELLAAQPDEVATIFFNKHASLASDHFHQHNLEAGELPSPAV
                     FVYTLPNILNGELAIRY"
     gene            complement(360..1490)
                     /locus_tag="PKV31_08670"
     CDS             complement(360..1490)
                     /locus_tag="PKV31_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNB92971.1"
                     /translation="MINAYCIGGSIVNGWGTDLDIHWDNILRNTTSLKHYTLPDGTAT
                     YASFLDNEQWEELLTKYGHHYTKAALLCIEAINKATEQLTIDWTTTTIVLATTKGDID
                     LYATLKTINHNTLSFLPHLAAQLNVYYKPQKPVQIISNACISGSQAIQFGASQIRNGF
                     AQTVIACGVDLLTLFTLQGFQCLKALSDKPAKPFDKDRNGVSLGEAASAIVLSNNPLP
                     FRDKAVVACTHGITTNDANHISGPSRTGEGLYKAMMSVNSGMNQLPDAVSLHGTGTLF
                     NDDMESIAMQRAGLEAIDCFGVKGAYGHTLGAAGVLESILSIYSLCFNIIPPTRGYSL
                     SGTPALLNVAKAPVLKPLNTVLKCSSGFGGGNCALLFKKIND"
     gene            complement(1503..1937)
                     /locus_tag="PKV31_08675"
     CDS             complement(1503..1937)
                     /locus_tag="PKV31_08675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB92972.1"
                     /translation="MPGTTGKTITSEHSLPVRFNEVDSMGIVWHGNYIQYLEDAREKF
                     GLKHNIDYLTIASQGFQIPVVDINIKYKKPLRYGDSVKVISTFIDTQAAKIIFNYQLF
                     HEASGELCTAAETTQVFINLKGELQVVPPAFFIEWKRKVGLL"
     gene            complement(2071..2769)
                     /locus_tag="PKV31_08680"
     CDS             complement(2071..2769)
                     /locus_tag="PKV31_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB92973.1"
                     /translation="MKKFRQKYQLFTVEGEKSVSELITSAPHLVKELIIRKSMATDYS
                     ALDNFNIFRAEDETMSKVSSMSTPPGIIAICSMAGYLESDEPDCDKYVLYLDDVRDPG
                     NAGTIIRTAEWFGFKKVFFSPGSIDLFHPKLIQATMGSFFRVFCREMDESSMDTLPRP
                     WVGATLDGKSLAEFKFPPTGTLFVGNESNGITPYLVQKLDSMVRIDESSTNRAESLNV
                     GMATAILMYEIAKR"
     gene            2872..>3549
                     /locus_tag="PKV31_08685"
     CDS             2872..>3549
                     /locus_tag="PKV31_08685"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNB92974.1"
                     /translation="MTLSSALLMSSCNVSRFLSAHQVLLTKETVVVEGEFKGGENTRL
                     KEGLVYLTNTVPFKREAVYSYFKNQGKIDSAGGVKRFFYNIISQEPVYFDSAQIEKTK
                     NNLTNYLKKEGYFDAEVRYTTSIKGKYGKVEYTAIPKQQYFFRDVTYEIREPEISIFL
                     SDSWNKSVLKRGKVVSDLNYQLEKNRISDILFNNGYYFFDPSLIGPLIADSIGHEVNV
                     AFRMNETD"
BASE COUNT         1031 a          713 c          743 g         1062 t
ORIGIN      
        1 catatcgaat ggctaattct ccatttaaaa tattgggcaa agtatataca aatactgcag
       61 ggctgggcaa ttctccggct tccaggttgt gctggtggaa atggtcagaa gcgagtgaag
      121 cgtgcttgtt gaagaaaatt gtcgctactt cgtccggttg ggcagccaat aattcgtgat
      181 gtttttctat taagaaatca catgcgatga agcctagttt gcataagaga ttcattttga
      241 aatatttggg atactttatt tctcttattc tatacagttc agtcaggttg tcggaaatgt
      301 gttccacatt accttcaaac ccgagacata ttgcagctac ctcttccagc ttcttcattt
      361 caatcattta tttttttgaa caaaagggcg caattgcctc ctccaaagcc cgaagagcat
      421 ttcaggacag tattcaaagg cttcaatacc ggcgctttgg caacattcaa taaagcgggc
      481 gttcctgaca ggctataccc acgtgtggga ggtatgatgt tgaaacaaag ggaataaata
      541 ctcaatatac tttcgagcac accggcagcg cctaaagtat gaccatatgc gcctttgaca
      601 ccaaagcaat caattgcctc gaggcctgca cgttgcatag cgatggattc catatcatcg
      661 ttgaaaagcg ttcctgtacc atgtagggaa acagcatccg gtagttgatt cattccgcta
      721 ttgacactca tcatggcctt gtacagaccc tcaccggtgc gggagggtcc gctgatatgg
      781 ttagcatcat tggttgtaat tccatgggta catgcgacga cagccttatc gcggaaaggc
      841 aacgggttgt tgcttaatac tattgctgaa gcagcctctc ccaatgaaac tccattcctg
      901 tccttatcga aaggtttggc aggcttgtca cttagcgctt ttagacattg gaaaccttgt
      961 aaggtaaata aagtcagcag atccacacca caggcaatga cagtctgagc aaatccattc
     1021 ctgatctgag aggctccaaa ttgaatggcc tgactacctg aaatgcaagc atttgaaata
     1081 atctgtacag gcttttgtgg cttgtaatat acattcaact gagctgccaa atgaggtaag
     1141 aacgaaaggg tattgtgatt aatagttttc aatgtagcgt aaaggtctat atcacccttg
     1201 gtagtggcta aaacgattgt ggtagtggtc cagtctattg ttaattgttc agtggctttg
     1261 tttattgcct ctatgcataa aagcgccgct ttggtgtaat gatgtccgta ttttgtcaac
     1321 aactcttccc attgttcatt atcgagaaat gaagcatacg tggcagtacc atcgggaagg
     1381 gtatagtgct tcaaagaggt tgtattcctt aggatattgt cccaatgaat gtcgaggtct
     1441 gttccccaac catttacaat ggaccctcca atacagtatg cgttaatcat cctattcact
     1501 gattacaata gacctacttt tcttttccat tcgataaaga atgcaggagg aacaacctgg
     1561 agttctcctt tcagattaat aaaaacctga gtggtttctg cagcagtgca caactcaccc
     1621 gaggcttcat ggaacaattg ataattaaaa ataatttttg cagcctgcgt atctatgaag
     1681 gtggaaatga cctttacgct gtcgccatat ctaagcggct ttttgtattt aatatttata
     1741 tccacaaccg gaatctgaaa tccttgtgaa gcaatagtca gataatcgat attgtgtttt
     1801 aagccgaact tctctctagc atcctcaaga tattgaatgt agttaccatg ccagacaata
     1861 cccatggaat cgacttcatt aaaccttact ggcagggagt gctcgctcgt gattgtttta
     1921 ccggttgttc caggcataaa tgaaatttat gggtaaaggt attaaatttt gaaaaaggtg
     1981 aaagattggg cggatcattc tttaccgtga aagttaaata aattaaacag cctcattgta
     2041 tttccgatca cctgttatgt aatagatagg ttatcttttt gcgatctcgt acatcaatat
     2101 agcagtggcc ataccgacgt tgagtgattc ggcgcggttg gtagatgatt catcaattct
     2161 taccatactg tccagttttt gtacaaggta cggtgtgata ccattggatt cattgcccac
     2221 aaataaagta cctgttggtg gaaatttgaa ttctgcaagc gattttccat ccaaagtggc
     2281 accaacccac ggacgaggta aagtatccat cgatgactca tccatctccc ggcaaaatac
     2341 tctgaagaaa cttcccatgg tagcctggat caacttagga tggaaaagat caatacttcc
     2401 cggtgaaaag aaaactttct taaagccaaa ccattcggct gtccttatga tggtgcctgc
     2461 attgcctgga tccctgacat cgtcgaggta taatacgtat ttgtcacaat ccggttcgtc
     2521 actttccaga tatccggcca tactacaaat tgcgatgatg ccaggcggtg tggacattga
     2581 ggaaactttg gacatcgttt catcctcagc ccgaaaaata ttaaaattat ctaatgctga
     2641 ataatctgtt gccatgctct ttctgataat caattcctta acaagatgag gagcagatgt
     2701 aattaattca ctgacacttt tctctccttc aacagtaaat aattgatact tttgtctaaa
     2761 ttttttcaaa tgaagcgacg ataagatctt cgcatgattt ttggtaagta attcattgaa
     2821 cattaactag tacgcttgta tcggtttgtc tgcaaatata tctttttttt gatgactttg
     2881 tcatcagctt tactcatgag tagttgcaat gtatcccggt ttttaagtgc tcatcaggtt
     2941 cttttgacaa aggaaacagt cgttgttgaa ggtgaattta aaggagggga gaataccagg
     3001 ttgaaggaag ggcttgtgta cctgacaaat acagtccctt ttaagcgtga agctgtatat
     3061 tcctacttta aaaatcaggg aaaaatagat tcggcaggag gggtcaaacg gtttttttac
     3121 aacatcattt ctcaggagcc tgtatatttt gactcagctc aaatcgaaaa aacgaagaat
     3181 aacctgacca attacctcaa gaaggaaggc tatttcgatg cagaagtgag atatacgaca
     3241 tcgattaagg gaaaatatgg caaggttgag tacaccgcaa tacccaaaca acagtatttc
     3301 tttcgcgatg tcacttatga gatcagggag ccggaaattt ctatttttct aagtgattcc
     3361 tggaataaat ctgtcctgaa gagaggaaag gtggtgtccg acctcaatta ccaattagaa
     3421 aaaaacagaa tatccgacat tcttttcaac aatggatact atttttttga tccgagcctt
     3481 atcggtccct tgattgcaga cagtatcggc cacgaggtca atgtggcatt cagaatgaat
     3541 gagacggac
//
LOCUS       DAOQMC010000236         3542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_13853_length_3542_cov_4.268426, whole genome shotgun sequence.
ACCESSION   DAOQMC010000236 DAOQMC010000000
VERSION     DAOQMC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13853_length_3542_cov_4.268426"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PKV31_08690"
     CDS             complement(<1..1173)
                     /locus_tag="PKV31_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB92975.1"
                     /translation="MNIFYPVLKKVVRFTLRFFFKRIYINKFDNLPDNTPILIACNHN
                     NAFADALFVGAYLSKLDMHFITRGDVFNPKMMWFFRLTNQVPIFRFRDGYENLKKNNN
                     SMEFCYDALGRNQRIIIFSEGDCKTEKHLRPIQKGTARMAFGAYDATGQDKTLIYPVG
                     INYIEPHSFRSSILISQGEPLRLIDYIPLYKENPHKAIKLLTDDLEFQLKKEVLHIEE
                     KEAEKLAQTGFEILDNAYPGTLFSLAKNSQLFNRLKMWSIQVNKEAKDPDSSWLVDLK
                     ELKDILISSRIKTKWPFFNKSFPLNMLLFILLFPIGILAQGFFYPPILLSKWLTIRFK
                     PRREFFLSIRIGISWAILTSWISIFFIIIGVEWSWRYGAAFVFLSPIIAKIGLIWWN"
     gene            1251..2195
                     /gene="miaA"
                     /locus_tag="PKV31_08695"
     CDS             1251..2195
                     /gene="miaA"
                     /locus_tag="PKV31_08695"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNB92976.1"
                     /translation="MKVLICIGGPTASGKTAMAIKLAKELNTEILSFDSRQIYHELNI
                     GVAKPDSNELSEVKHHMIGNVSVERHFNAGTYEREAGKVLDSLLESNNSVVAVGGTGF
                     YLNALIDGLDDMPEVSAIVNASVEEDYRLHGLDWLRQYVIDHDPEYASEADLLNPVRL
                     LRAAKIIRSSGKAFSYYRRGLKRENYFKVLKYFLEPDREKLYHRINLRVDDMMTAGLL
                     NEAKSLQHLRYLKALNTVGYTELMDYLDGKTSLEEAIELIKRNTRRYAKRQFTWFRNQ
                     GDWISLNPESLEENIKIIKQQLYDLENGSNDRRSECDE"
     gene            2146..2571
                     /locus_tag="PKV31_08700"
     CDS             2146..2571
                     /locus_tag="PKV31_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNB92977.1"
                     /translation="MIWKTEATIEGLNAMSENTMGSHLGMEFTGIGDDFLEARMPVDH
                     RTKQPMGLLHGGASAALAETLGSVASYLCIEDISKETAVGLNITADHLRLISKGWVTG
                     IARPIKLGRKIHLWSIEIKNEKDQLVCISKLSVMIIPKP"
     gene            complement(2555..>3542)
                     /gene="gap"
                     /locus_tag="PKV31_08705"
     CDS             complement(2555..>3542)
                     /gene="gap"
                     /locus_tag="PKV31_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNB92978.1"
                     /translation="GFGRIGRLTLRNLLSNPEVEVVGINDLTDNATLAHLFKYDTTHG
                     IYPGEVTSDETSIYINGKKIHATSIRDPKELPWAELDIDVVLECTGIFLTKEKASWHL
                     EAGAKRVVLSAPAGEEVKTIVLGINGHEINDETKIYSNASCTTNCLAPVAKIIVDNWG
                     IVSGSMSTVHAYTADQNLQDSPHKDLRRARAAAQNIVPTSTGAAKAAALVVPELIGKM
                     TSMALRVPVITGSIVDLTIFTEKSLTREEVNETFKKASEGKFKGIIEYSELPLVSSDI
                     IGNKYSAIFDSKLTLVRDNMLRIFAWYDNESGYSARLAELGAAVCQPAMASV"
BASE COUNT         1035 a          648 c          793 g         1066 t
ORIGIN      
        1 attccaccaa atcaaaccga tttttgctat tatcggagat aagaacacaa aggccgctcc
       61 atatcgccaa ctccattcta caccaatgat aataaagaat atggaaatcc atgatgtcaa
      121 aatagcccag gaaataccta tacggataga cagaaaaaat tcacgtcttg gtttgaatct
      181 gattgtaagc cattttgata ataatatcgg aggatagaaa aaaccttgag ctaatatgcc
      241 gatagggaat aataggatga agagtaacat attgagtggg aatgatttat tgaagaatgg
      301 ccattttgtt ttgattctgg aactaatcag aatatccttg agttctttaa gatcgactaa
      361 ccaggaagaa tcagggtcct tggcttcttt attcacttgt atcgaccaca ttttcaacct
      421 attgaacaat tggctgtttt ttgccaggct aaatagcgtg ccgggataag cgttgtccaa
      481 aatttcaaat ccggtctgtg ccagcttttc agcctctttc tcttcaatat gcaggacttc
      541 cttttttaat tggaactcaa ggtcatcagt gagtaacttt attgccttgt gtggattttc
      601 tttatatagg ggtatataat caatcaacct tagaggctcg ccttgtgaga tcagaatgct
      661 tgaacggaaa ctgtgaggtt caatataatt aataccaaca ggatagatca gggttttatc
      721 ctggccggtc gcatcataag caccgaaggc catgcgagct gttcctttct gtataggacg
      781 caagtgtttt tctgttttgc agtctccttc cgagaaaatg atgatgcgtt ggttacgtcc
      841 caatgcatca taacaaaact ccatggagtt gttgtttttc ttaagatttt catatccgtc
      901 tctgaatctg aatattggta cttggttagt aagtctgaaa aaccacatca tcttcggatt
      961 gaatacatcg ccacgtgtga tgaagtgcat atcaagtttg gatagataag cacctacaaa
     1021 aagagcatcg gcaaaagcat tgttatggtt gcaggcgatc aaaataggcg tattgtccgg
     1081 tagattgtcg aacttattga tgtaaatacg tttgaaaaaa aacctgaggg taaatcgtac
     1141 tactttcttt aataccggat aaaatatatt cattaattat aatatgaaaa tcggcttatt
     1201 gcctgaatat ttattttatt atttattttg tgcgaaaata aagattcttt ttgaaagtat
     1261 taatatgtat cggtggcccc acggcaagtg gcaaaactgc catggctatc aagctggcaa
     1321 aagagttgaa cacggagatt ctttcattcg atagccgtca gatatatcat gaattgaata
     1381 tcggggttgc caagcctgat tcgaatgaac tttcagaggt gaagcatcac atgataggca
     1441 atgtgtccgt tgaaaggcat tttaatgctg ggacatatga acgggaagcg gggaaggttc
     1501 tggactcttt attggagtcg aataattctg tagttgcagt aggtggtacc ggtttttatc
     1561 ttaatgcttt gattgatggc ttggacgata tgcccgaggt atctgctata gtgaatgcat
     1621 cggttgaaga agattataga ctccatggtt tggattggtt gaggcagtat gttatagatc
     1681 atgatccgga atatgcatcc gaagctgact tgctgaatcc ggttagattg cttcgggcgg
     1741 caaaaataat aaggagtagt gggaaggcat tttcatatta tcggcgtgga ttaaaacggg
     1801 aaaattactt taaggtgttg aaatattttc tggaaccgga tcgtgaaaaa ctttaccata
     1861 gaattaattt aagagttgat gatatgatga cggccggttt attaaatgag gcaaagagcc
     1921 ttcagcattt aaggtatttg aaggctctta atactgtagg ttatactgaa ttaatggact
     1981 atctggatgg taagaccagt ctggaggagg cgattgaatt gataaagaga aatacccgaa
     2041 gatatgccaa acgccagttt acctggttca ggaatcaagg agattggata tctttaaacc
     2101 cagaatcttt agaagaaaat ataaaaataa taaaacaaca attatatgat ctggaaaacg
     2161 gaagcaacga tagaaggtct gaatgcgatg agtgaaaata cgatggggtc tcatctggga
     2221 atggaattca caggaatagg tgatgatttt cttgaagcca ggatgccggt agaccaccga
     2281 accaaacagc caatgggcct tttgcatggg ggagcctcgg cagctttggc tgaaacattg
     2341 ggcagcgttg catcctatct atgcatagaa gatatttcca aagaaacagc tgttgggttg
     2401 aatatcacgg ctgatcacct tcgtcttata tcaaaaggat gggtgaccgg tatcgcccga
     2461 cccatcaagc tgggtcggaa gatacatctt tggtctattg agataaaaaa tgaaaaagat
     2521 caactggtat gtatatctaa actgtcggtc atgattatac cgaagccata gcaggttggc
     2581 aaactgctgc ccccaattca gcaagacgcg ctgaatatcc tgattcgtta tcgtaccatg
     2641 cgaaaattcg gagcatatta tccctgacga gagtaagctt gctgtcgaag attgccgaat
     2701 atttattgcc aatgatatcg gaggaaacga gaggcaattc actatattct atgataccct
     2761 tgaattttcc ttctgatgct tttttgaaag tttcattcac ttcttctcgg gtcagagatt
     2821 tttctgtaaa aatggttagg tcaacaatcg atcctgtgat cacaggtacc ctcagagcca
     2881 tggaagtcat ctttccaatc agttcaggca ctaccaaggc agctgctttt gctgcacctg
     2941 ttgatgtggg tactatattt tgtgcggctg cacgtgctct tctaaggtct ttgtgcggag
     3001 aatcctgaag attctgatca gcagtatagg cgtgtacagt tgacatggat ccggatacga
     3061 tgccccagtt gtccacaatt attttggcaa ccggtgcaag gcagttggtt gtacaggacg
     3121 cattggaata tattttagtt tcatcattta tttcgtgtcc attaatccct aatacgatgg
     3181 tcttgacttc ctcacctgct ggagcagata ggacaaccct tttagcccct gcttccagat
     3241 gccagcttgc tttttccttg gttaggaaga tacctgtaca ctctaaaaca acatcaatat
     3301 caagttctgc ccatggcagt tcctttgggt cgcggataga agtggcgtgt attttttttc
     3361 cattgatgta aatggaagtc tcatcactgg taacctctcc cggatatatt ccatgtgtcg
     3421 tatcgtactt aaataagtgg gcgagagttg cattgtcagt caggtcattt attcctacaa
     3481 cctcaacttc cgggttggat agaaggtttc tgagcgttaa tcggccaatt ctgccaaagc
     3541 ca
//
LOCUS       DAOQMC010000237         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14072_length_3507_cov_4.502317, whole genome shotgun sequence.
ACCESSION   DAOQMC010000237 DAOQMC010000000
VERSION     DAOQMC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14072_length_3507_cov_4.502317"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..380
                     /locus_tag="PKV31_08710"
     CDS             87..380
                     /locus_tag="PKV31_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92979.1"
                     /translation="MNIYYKKPVITEEKRVIIEQVYELGEEILVNGIIRKELKVIVEI
                     YPSIELLLETISIATMNYNNVSLHGHKTVMQLEMEKKALVDLQVMLSEMLDKK"
     gene            642..1979
                     /gene="ffh"
                     /locus_tag="PKV31_08715"
     CDS             642..1979
                     /gene="ffh"
                     /locus_tag="PKV31_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNB92980.1"
                     /translation="MFESLNEKLEGAFKSLKGEGKLTELNVATSIKEIRRALVAADVN
                     YKIAKEFTDKVKDQAMGEKNVLKAVKPGELMVKIVMDELIELMGGASEGISLSGNPAI
                     ILISGLQGSGKTTFTGKLSLYLKTKRSKKVLVTAGDVYRPAAIDQLTVLSQQVGVDIY
                     KEPENKNPVQIATNAIQYAKDNGFDVVIIDTAGRLAIDEAMMNEIEAIKKAVNPSETL
                     FVVDSMTGQDAVNTAKAFNDKLNFDGVVLTKLDGDTRGGAALSVKYTVGKPIKFISTG
                     EKMEDLDIFYPDRMAQRILGMGDIVSFVERAQQQFDEEEAARIEKKIKKNKFDFNDFL
                     SQINQIKKMGDIKSLMNMIPGMSKLTANLDIDEKAFSKVECIIQSMTPQERANPELLN
                     PSRKRRIAKGAGKDINEINMFIKQFDQMKNMMHNMSIGKMGNMGMPKMGRFRR"
     gene            complement(1990..3378)
                     /locus_tag="PKV31_08720"
     CDS             complement(1990..3378)
                     /locus_tag="PKV31_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNB92981.1"
                     /translation="MNIYLHLAYLTASILFVIGIKMLNKTKTARNGNLVSAVGMLIAI
                     LATVSQVQSITYIDIFVAFLIGSAIGLIFAYKVKMTQMPEMVAIFNGFGGLASVAVAL
                     SDYWLHTQQQSIELSSITVVSIILSIFIGGITFTGSMIAFLKLNGSISGRAIVFKGQN
                     PLNLMLLIIGIILSVLIVMYTSDPSYILLLTILSLVLGVLVVIPIGGADMPVVISLLN
                     SYSGMAACATGFVLNNNVLIIAGALVGASGIVLTDIMCKAMNRSLINVLLGGFGQTSS
                     SVQAGSSQDIVIKEVGVEEAAQIFDSVSSLIIIPGYGMAVAQAQHAIRELYELCEKKG
                     VSVRFAIHPVAGRMPGHMNVLLAEANIPYDKLVEMEAINDDFTNTDLVFVVGANDVVN
                     PAAKNSPGSPIYGMPVLNAELARTVIVSKRGMNPGYAGIENELFGADNCLMLFGDAKQ
                     TITKLVAELKEL"
     gene            complement(3400..>3507)
                     /locus_tag="PKV31_08725"
     CDS             complement(3400..>3507)
                     /locus_tag="PKV31_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HNB92982.1"
                     /translation="SLSKWLGLAAMVLATVNVVGGYAVSDRMLKMFKKK"
BASE COUNT         1118 a          753 c          726 g          910 t
ORIGIN      
        1 ggattgaaaa ataatgagga atattttttt ggacctgaga tagggtgtat ttttaaatat
       61 ttcgaaccaa aataagcgat ataaaaatga atatttacta caaaaagccg gtcattaccg
      121 aagaaaaaag agtaattatt gaacaggtat atgagctcgg agaggaaata cttgtaaatg
      181 gaattatcag aaaggaattg aaagtaatag tggaaattta cccttctatc gaattattgc
      241 ttgaaacaat atcgatagca accatgaact ataataatgt ttccctgcat ggtcacaaaa
      301 ccgtgatgca attggaaatg gaaaagaaag cattagtgga tttacaagta atgttatcgg
      361 aaatgctgga taaaaaataa ccggtttgct tttacccggc aaattgacat ttcgttcgtt
      421 ttttgttcaa tacttccggc ttcttgattt tttttattta aaccttgacc ttcaagtttt
      481 aaacaaagtt gcatatgcct tgtattaata acctgcctag ctgaacttta cactacattt
      541 ggccatggat tctcctgttc ggattatcga atcattattt ctatatactt tcccctctct
      601 attttttaac tatatttgca cctcaaaatt gaaagacaga catgtttgaa agtttaaatg
      661 aaaaactgga aggtgccttc aaatcactaa agggtgaagg caagttaacc gagcttaatg
      721 tcgcaacctc catcaaggaa attcgtcgtg cattggttgc tgccgatgtc aattataaga
      781 tagccaagga atttaccgat aaggtcaaag atcaggctat gggtgaaaaa aacgtcctga
      841 aagccgttaa accgggtgag ctcatggtca aaatcgtcat ggacgaattg atcgagttga
      901 tgggcggggc atccgaaggt atttcacttt ccggcaatcc ggccattatc cttatttcgg
      961 gattgcaagg ttcgggaaag accactttta ccggtaaact ttccctttat ttaaaaacta
     1021 aacgtagtaa aaaagtactt gtaacagcgg gtgacgtgta tagacctgct gcgattgatc
     1081 agctgaccgt gctcagtcag caggttggtg tagatattta caaggaaccg gagaataaaa
     1141 atcctgttca aatcgccact aatgccatcc aatatgccaa ggacaatggt tttgatgttg
     1201 tcatcatcga tacagccggc cgtctcgcca tagatgaggc tatgatgaat gaaatcgaag
     1261 cgattaaaaa agcagtcaat ccgtctgaga ctttgtttgt ggtggattcc atgacaggac
     1321 aggatgctgt caacacggct aaagctttca acgacaagct caatttcgat ggcgtcgtcc
     1381 ttaccaaact tgacggtgat acacgcggtg gtgccgccct ttcggttaaa tatactgttg
     1441 ggaagccaat taaattcatc tccaccggtg agaaaatgga agatctcgac atcttctatc
     1501 cggacaggat ggctcaacga attctgggca tgggagatat cgtttcattt gttgaacgtg
     1561 ctcagcagca gtttgatgag gaagaagcag cccgaatcga gaagaaaatc aagaagaata
     1621 agtttgactt caacgacttc ctctctcaga tcaatcagat aaaaaagatg ggtgacatca
     1681 aaagtctgat gaacatgatt cccggcatga gcaaactgac tgccaacctg gacatcgatg
     1741 aaaaagcctt cagcaaggtg gagtgcatca tacaaagtat gactcctcag gaacgtgcca
     1801 atcctgaatt gctcaaccca agccgtaaac gacgtatagc caagggggcc ggtaaagaca
     1861 tcaacgaaat caatatgttt atcaaacagt ttgatcagat gaaaaacatg atgcacaata
     1921 tgtccatagg taaaatgggc aatatgggca tgccaaaaat gggacgattc agaagataac
     1981 cttacgttgc tataattcct tcaactctgc aaccaactta gttatggtct gcttggcatc
     2041 accaaagagc atcaggcaat tgtccgcgcc aaacaattca ttctcgatgc cggcgtaacc
     2101 cggattcatc cccctcttgg atacaatcac cgttctggcc aattccgcat tcagtaccgg
     2161 cataccatag attgggctac ccggtgaatt cttcgcagca ggattgacta catcattagc
     2221 cccaacgaca aatacaaggt cagtattggt aaaatcatca ttgatggctt ccatttccac
     2281 caatttgtca tacgggatat tggcttcggc gagcaaaaca ttcatgtggc caggcatgcg
     2341 accggcaacc ggatgtatgg caaagcgcac actgacacct ttcttttcac acaattcata
     2401 gagctctctg atggcatgct gagcctgtgc cacggccatt ccatatcccg gaataataat
     2461 cagcgaactt actgaatcga agatctgggc agcttcctcc acgcccacct ctttaatcac
     2521 tatatcctga ctgcttcctg cctgcacaga tgacgatgtc tgtccaaatc cgcctaaaag
     2581 aacattgatt aatgatcgat tcattgcctt gcacatgata tccgtaagga caatacctga
     2641 agctccgaca agggcacctg caatgatgag gacattgttg ttgagaacaa atccagtcgc
     2701 acaagccgcc attcccgaat acgagttgag cagtgaaata accacaggca tatcggcacc
     2761 tcctatggga atcacaacca atacgcccag taccagagaa agaatcgtca gaagtaatat
     2821 gtaggatggg tcggaggtgt acatcactat caaaacagaa agaataatgc caatgatgag
     2881 gagcatcaaa ttgagtggat tctgaccctt gaagactatt gcccgaccac tgatactacc
     2941 attgagcttg agaaatgcaa tcatggaacc ggtaaaagta atccctccaa taaaaatact
     3001 caggattatg gaaaccactg tgatactgct caattcaata ctttgctgct gtgtatgcaa
     3061 ccaataatca gatagagcaa ccgccacgga tgccagacca ccaaaaccgt tgaagatagc
     3121 taccatctca ggcatctggg tcatcttaac tttataggca aatatcagac ctatcgccga
     3181 cccgattaaa aaagcaacaa aaatatcaat ataagttatc gactgaacct gtgaaactgt
     3241 tgctaaaatg gctatcaaca tacccacagc ggaaacgaga ttcccatttc tcgctgtttt
     3301 cgttttattc aacatcttga taccgatgac aaaaagtatc gaagcggtca gataggctaa
     3361 atgtagataa atattcatat caggaaggga atggtgaggt tatttctttt tgaacatttt
     3421 aagcatacga tcagacacag cataaccacc gacgacattg accgtagcaa ggaccatcgc
     3481 agccaggccg agccatttgc ttaggct
//
LOCUS       DAOQMC010000238         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14181_length_3494_cov_5.393138, whole genome shotgun sequence.
ACCESSION   DAOQMC010000238 DAOQMC010000000
VERSION     DAOQMC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14181_length_3494_cov_5.393138"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKV31_08730"
     CDS             complement(<1..258)
                     /locus_tag="PKV31_08730"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNB92983.1"
                     /translation="MMIPYLTLDDTITAISTAPGVGAIGVVRLSGRDAVSVASRIFKG
                     KNLSRQKSHTAHFGKIIDLEGREVDEVVTTIFLHPNSYTGED"
     gene            1000..1611
                     /locus_tag="PKV31_08735"
     CDS             1000..1611
                     /locus_tag="PKV31_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB92984.1"
                     /translation="MLLDNSTEEKIKESARKVFLKKGYAATRTRDIAEGAGINLALLN
                     YYFRSKEKLFELIMLETMFSFMQKMAVVLNDEDSKLEKKVELIASNYIDFFTEEPNVP
                     IFVLNEMHKNPRLLFDTLPVRELFFNSVFFRQYQTQVAKGKIKEGNPANFLINMMSLI
                     IFPFVGSPIFKLITGTLDVQYLELMQERKKLIPRWVKAMMKSM"
     gene            1663..2946
                     /locus_tag="PKV31_08740"
     CDS             1663..2946
                     /locus_tag="PKV31_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB92985.1"
                     /translation="MIKSFFRNKNLWLIGILMTCGQLLSAQVSNPLTLDGCIEMAKIN
                     YPTIRQFSLIEQAREYSLDNARKAYLPQFTLMGQATYQSDVTQIPVSLPGLNIPVISK
                     DQYRLYGEVSQSITDLFTVRNQQKVIHANADIEIQKTEVELYKLRERINNLFFGILMI
                     DAQIGQVELLKKDIKTGIEKTGVAIANGVALKSAADNLEAELLKVDQRAIELKAARKG
                     YTEMLSLFIGKPVDEQSILQAPAAPEIVHTVNRAELKLFDLQRHAFDAQEELITARNL
                     PRISVFLQGGIGRPALNMLHSDLKGYYIGGLRLAWNFTGFYTYMNEKAMLVNQGKLID
                     VQRETFTFNTNLQIKQQNAEIIKMKELLESDKAIVVLRANVTSTTSNQLALGTATTND
                     YLVAINAEDQARQNMLLHQIQLLMIGYHLKTITGN"
     gene            2987..>3494
                     /locus_tag="PKV31_08745"
     CDS             2987..>3494
                     /locus_tag="PKV31_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNB92986.1"
                     /translation="MAKMIRKNTIAAGIMIACMIVGIVSCNKDKDNFDASGSFEAIET
                     IVSAEANGRIERLEIEEGQELEAGRNIGFIDSTQLFLKKKQLEAQIVAILGRKPDIPV
                     QLSALQEQLRTAERERVRTVGLVKGEAATAKQLDDLDAQIALLKKQIEAQKSTLSISS
                     TGLSKEAMP"
BASE COUNT         1099 a          641 c          780 g          974 t
ORIGIN      
        1 atcctcaccg gtatagctgt tgggatggag aaaaatggta gtcaccacct catcaacttc
       61 cctaccttcc aaatcgatga tcttcccaaa gtgtgccgtg tgactttttt ggcgggacaa
      121 attctttccc ttgaagatcc tgcttgcaac gcttaccgca tccctgccac taagccgtac
      181 aacaccgata gcgcccactc cgggagctgt ggaaatggca gtaatggtat catccagagt
      241 caagtatggt atcatcataa taaagggtga aattagtaaa aaaggggaat tcattgtgaa
      301 aggaaagaaa ttggtgtgga tcacgtatgc cctttactct ttccggacta aaagaggaga
      361 agttgtcaat gaggcatgta actcggatca gctatcttcc aataattatt taagtctaat
      421 gtttcgaatg tccttaattc ttgataaata cttatcaaat gtcaaaactg tggaatgatt
      481 ttgaactatt aatgattgat aagaataggt atataaaaaa cccgatgctt gttaaatact
      541 gcttaatggt atacttcagc ataatccttt tactttttta ctgttcatcg tcatatatgc
      601 caccgaagta atcaaatatt atggccattg gctcatcatc ctattaattt gtagataaag
      661 cacaaagccc gggtgacggt ttggctctta agacataaat gtggttgagc ctgctttttc
      721 taattattca atgccattag cgttatgtca tgctccaatt taaattgttg taatcccgcg
      781 atccgaaaag gttgtaattc aggcaaaggc aaattgtaaa ttcaatctga gggttcaacc
      841 attgcaatta ataggtatta atttttcgct tcattttcag atttgataaa caggaataaa
      901 atatttaaac aaatgattaa aatatttggt taatttggtt aattatgtat atctttgttt
      961 gataaaggcc tttcatccat ggtgaaaaag agagaaaaca tgttattaga caactcgacc
     1021 gaggaaaaga taaaggaatc ggcgcgtaag gtcttcttga agaagggcta tgctgcaact
     1081 cgtacgagag atattgcgga gggagcaggt atcaaccttg ccctgctcaa ctattatttc
     1141 cgaagtaagg aaaagctgtt cgaactgatt atgctggaga caatgttcag ttttatgcag
     1201 aaaatggctg tagtgttaaa cgatgaagat agcaaattag agaagaaggt tgaattaata
     1261 gcttcgaatt atatagactt tttcactgag gagcctaatg tgcccatctt cgtgttgaat
     1321 gagatgcaca agaatccacg attgttgttt gatacgctac ctgtcagaga attgtttttt
     1381 aattcggttt ttttcaggca atatcagaca caggtagcca aagggaagat aaaagaaggc
     1441 aatccagcta atttcctgat caacatgatg agcctgatca tttttccctt cgtgggtagt
     1501 ccaattttta agttgattac cggaacttta gatgtacagt atttggaatt gatgcaggaa
     1561 cgtaaaaagc tgattccccg gtgggtaaag gcaatgatga agtcgatgta atattttttt
     1621 gaaacaaatt aatcaaatga ttaaatctaa atattaaact gtatgattaa gtcatttttc
     1681 cgaaacaaaa atctatggct aatcggcatc cttatgactt gtggtcagtt attatctgcg
     1741 caagtaagca atccattgac actggatggt tgtatagaga tggcaaaaat taattatcca
     1801 accattcgtc agttttcatt gatcgagcag gcgcgggaat attcgctaga caatgcccgg
     1861 aaagcttatt tgcctcaatt taccttgatg gggcaggcta cctaccagtc tgatgtgacc
     1921 caaattcctg tctccttgcc cggcctcaat attcctgtta tcagtaagga tcagtaccgc
     1981 ctctatggag aagtgtcgca atccattact gatctattta ctgtcaggaa tcagcagaag
     2041 gttatccatg ccaatgctga cattgagata caaaagacag aggtggagtt gtacaagttg
     2101 cgcgagcgca tcaacaatct gttttttggc atattgatga ttgatgcaca gattggtcag
     2161 gttgaattgc tcaagaaaga tatcaaaacc ggcattgaaa aaaccggggt cgccattgcc
     2221 aatggagtag cattgaagag tgcagcggat aaccttgaag cagaattgtt gaaggtagac
     2281 cagcgtgcca tcgagttgaa agctgcacgt aaaggttata ctgaaatgct gtctttgttt
     2341 atcggtaagc ctgttgatga gcaatcgatc ttgcaggcgc ctgcagcacc cgaaattgtg
     2401 catacggtta atcgtgcaga attgaagttg ttcgatcttc aaaggcatgc ctttgacgca
     2461 caggaagaac tcatcactgc caggaatctg cctcgtatca gtgtttttct tcagggcggt
     2521 attggtcgac cggcactgaa tatgcttcac agcgatctga aaggctatta tataggaggt
     2581 cttcgtctgg catggaattt taccggtttt tatacctata tgaatgaaaa ggcgatgctt
     2641 gtcaaccagg gcaagctgat tgatgtgcaa agagaaacct ttacgtttaa caccaatcta
     2701 caaatcaaac aacaaaatgc ggaaattatc aagatgaaag agctacttga atcagacaaa
     2761 gcaattgtcg tactgcgtgc taacgtgaca agtactacca gcaaccagct tgcattgggc
     2821 acggccacta ccaatgacta tctcgttgca atcaacgcgg aggatcaggc gcggcaaaat
     2881 atgttgctac accaaatcca actattgatg ataggatatc atttaaaaac cataacaggc
     2941 aattgaaggc aggatgtgta attctaaaaa tcaaaaatta acaaaaatgg cgaagatgat
     3001 aaggaagaat acaatagcag caggtataat gattgcctgt atgattgtgg ggatagtatc
     3061 gtgcaacaag gacaaagata acttcgatgc ttcaggaagt tttgaggcga tagaaaccat
     3121 tgtctcagca gaggcaaatg gaagaataga gcgtttggaa attgaagaag gacaggaatt
     3181 ggaagcaggg aggaatatag gcttcattga cagcacacag ctttttttaa agaaaaagca
     3241 gttggaggca cagattgtcg ctatacttgg aaggaagccg gacattccgg ttcaactatc
     3301 tgcattgcag gaacaactca ggacagccga gcgtgaacgt gtgaggactg tcggtttagt
     3361 caaaggtgag gccgccacag ccaagcaatt ggatgacctc gatgcgcaga tagctttgct
     3421 gaaaaagcag atagaggcgc aaaaatctac cctgagtata tcgagtacgg gactcagcaa
     3481 ggaagccatg cctc
//
LOCUS       DAOQMC010000239         3491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14202_length_3491_cov_4.556170, whole genome shotgun sequence.
ACCESSION   DAOQMC010000239 DAOQMC010000000
VERSION     DAOQMC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14202_length_3491_cov_4.556170"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKV31_08750"
     CDS             <1..333
                     /locus_tag="PKV31_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92987.1"
                     /translation="MEDQLITVQKTLHDLGAQDIPTLIVGNKIDAYKKNLFDDFLSNA
                     EKKEILAEQLEHFQRNFPQPWIFISAYTGENLDALKSLLADKVKEIYETKFPHQFQKE
                     YNQNSFKQ"
     gene            344..2566
                     /locus_tag="PKV31_08755"
     CDS             344..2566
                     /locus_tag="PKV31_08755"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNB92988.1"
                     /translation="MLNVNQTWKKWAGMLFLALLSISVSTAQVKMTKKKSKETKAVAP
                     QNVPMSQINDYSGYYPYVEYGKFDNGKMWTFSYPPVQYFKETYGFTPSEEWLENMRMS
                     ALRFSTYCTASFVSEDGLVMTNHHCGRQSVTEVTKDGEDLPKDGFYAATLEEERKVPG
                     LFVDQLIAMKDVTSDILMAMSNGKDDDEKNKLKNEKINELVKENETATGIKVQVHTFY
                     NGSIYQMYTYKRYSDVRLVMAPETDLGYFGGDPDNFTYPRYSLDYTFFRVYDEDGKPL
                     KTKYHYTWSESGAKENEPLFVVGNPGTTQRLSTVSMLEYYRDYTMPHTLDLLNMLGST
                     YKEYIDSHPESKLELTDTYFGYMNSLKAYNGMHGGLKNPDLMGKRASFEKAFKTAVLE
                     KPELKAKYGDIWAKINASRNQMKQYFGKQIMFSPSGSTSKLFALGARAIDIATEMKKP
                     ENERTQGNSDEQLKAKIEKLKLSKSYYPQIEEKAIEFQLTKISKWSGNKMRMIDGTNN
                     PVMIPAQAKAIVANSVVANHEKLMALFNEGPDAVLNSNDPVLAYCKMSKDSLAYYKTI
                     IDPIAAEENKNIQLLGQAFYEVYGTSTPPDANFTLRIADGVLKTYDYNGTTAPEFTTF
                     YGLFDRYYSYGKKEPWNLPQRWLDAEKYMNKSTKYNYVTTHDIIGGNSGSAMVNSKGE
                     VVGLIFDGNIESLPGRYIFDMKSNNRCVAVHSDGIIETLRSVYKAGRIADELKNAKIK
                     "
     gene            2664..>3491
                     /locus_tag="PKV31_08760"
     CDS             2664..>3491
                     /locus_tag="PKV31_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB92989.1"
                     /translation="MKVCGFTIIRNAVKYDYPVVEAITSILPACDKFIVLIGNSDDDT
                     LDLIQSIHSEKIEIHHSVWDDSLREGGRVLALETNKALDLIPSEYDWAFYIQSDEIIH
                     EKYIPAIREAMHRWKDERQVEGLLLKYLHFYGSYDYVGTSRKWYRNEIRIIKNEKSIR
                     SYKDAQGFRKNGSKLNVVPINAWVYHYGWVKDPLVQARKHSDFNKLWHNDDYVARITQ
                     EIYNYSDIDALGRFEDSHPEVMKVRIAAKNWHFDGTRISRKSGIKDKLLFFFEKATGI
                     "
BASE COUNT         1170 a          696 c          722 g          903 t
ORIGIN      
        1 atggaagacc agctgattac cgtacaaaag accctccatg accttggcgc ccaagatatt
       61 cccaccctga ttgtgggcaa taagattgat gcgtataaaa aaaatctttt tgatgacttc
      121 ctttccaatg cggagaaaaa agaaatcctg gctgaacagc tggaacactt ccaaagaaat
      181 tttccgcaac catggatttt catttcggct tataccgggg aaaatttgga tgccctcaaa
      241 tcactgcttg cggataaggt taaggaaata tatgaaacca aatttcctca tcaattccaa
      301 aaggaatata accagaattc atttaaacaa taaattatag actatgctga atgtaaatca
      361 aacatggaaa aaatgggccg gaatgctttt cttggctttg ctatccatct ccgtctctac
      421 cgctcaggta aaaatgacga agaaaaaaag caaggagaca aaagcagttg ctccgcaaaa
      481 tgtaccaatg tcacaaatca atgattattc cggatattat ccttatgtgg aatacggtaa
      541 gtttgacaac ggtaagatgt ggaccttttc ttatcctcct gtacaatatt tcaaagaaac
      601 ctatggcttc accccttctg aagaatggct cgagaatatg aggatgtcag cgctcaggtt
      661 ttctacatat tgtactgctt ccttcgtctc tgaagatggg ctggtcatga ctaatcacca
      721 ttgtggtcgt caaagtgtca ctgaagtaac caaggatggc gaggatctcc ctaaagatgg
      781 tttctatgca gcaacattgg aagaagagag aaaggttccg ggtctttttg tagatcaatt
      841 aatcgctatg aaagatgtca cttctgatat ccttatggca atgtcaaacg gaaaagacga
      901 cgatgagaaa aataaactga agaatgaaaa aatcaacgaa cttgtaaagg aaaatgaaac
      961 agctaccggc attaaagttc aggtccacac tttctacaat ggaagtatct atcaaatgta
     1021 cacttataaa agatattctg atgtgagact ggtcatggcc cccgaaaccg atctgggata
     1081 ctttggcggt gaccctgata attttactta cccacgttat agccttgact acaccttctt
     1141 cagagtatat gatgaagatg ggaaaccact gaaaaccaaa taccactata cctggtccga
     1201 atcaggagca aaagaaaatg aacctttgtt tgtagtaggc aaccctggta ccacccagcg
     1261 cctcagtacg gtttccatgc tggaatacta cagggattat acgatgccac acaccctcga
     1321 ccttctcaat atgctgggct ctacctataa agagtatatt gactctcacc ctgaaagcaa
     1381 actcgaactt acggatactt attttggcta tatgaatagt ttgaaggcat ataacggaat
     1441 gcatggcggt ctcaagaatc cagatttgat gggcaaacgt gcttcttttg aaaaagcttt
     1501 caaaacagca gttttagaaa aaccggagct taaggcaaaa tatggtgata tctgggctaa
     1561 aatcaatgca tcaagaaatc agatgaaaca atattttggt aagcagatta tgttctcacc
     1621 atcgggttca acctctaaat tatttgctct gggtgcaaga gcgattgata tcgccacaga
     1681 gatgaaaaag cctgagaatg agcgcacaca gggcaatagc gatgaacaat taaaagctaa
     1741 aattgaaaaa ttgaaactaa gcaagagtta ttatcctcaa atcgaagaaa aagcaataga
     1801 gtttcaattg accaaaatca gcaaatggtc cggcaataaa atgcgtatga ttgatggaac
     1861 caacaatccg gtaatgattc cggcgcaggc taaagctatt gtcgcaaatt ccgttgtagc
     1921 caaccacgaa aaattaatgg cgcttttcaa tgaaggtccc gatgccgttc taaattccaa
     1981 cgatcccgta cttgcttatt gtaaaatgtc taaagacagc cttgcttact ataagacaat
     2041 cattgatcct attgcagctg aggaaaacaa gaatatccag ctattgggcc aggcattcta
     2101 tgaagtatat gggaccagca caccaccgga tgccaacttc acactcagaa ttgctgatgg
     2161 cgtgttgaaa acatatgatt ataatggcac aactgcgcca gaatttacta ctttctacgg
     2221 cctttttgac agatattatt cctatggtaa aaaggaacca tggaatctgc cacaaagatg
     2281 gctcgatgct gaaaagtata tgaacaagag caccaaatac aactatgtta ccacccatga
     2341 catcattgga ggtaacagtg gcagtgccat ggtcaacagc aagggagagg tagtaggatt
     2401 gattttcgac ggaaacatcg aaagtctccc tggcaggtac atcttcgata tgaagagcaa
     2461 caaccgttgt gtcgcagttc attctgatgg cattatagag actcttcgct cagtctataa
     2521 ggcaggcaga attgcagatg aattgaaaaa tgcaaaaatt aaataaggca taaaaagcaa
     2581 gaaaaagcaa gaatggcctg gctttcaaca aagttcaggc cattttattt aatatattca
     2641 atccttttta ctaaatttca tcaatgaaag tctgtggatt taccatcatc agaaatgccg
     2701 tcaaatacga ttatccggtc gtggaagcta tcacaagcat acttcccgct tgcgataagt
     2761 tcattgttct tattggcaat agcgacgatg atacattaga cctgatccaa tccatccatt
     2821 cggaaaagat tgagatccat cactcggttt gggatgattc attgagagaa ggtggccggg
     2881 tcctcgcatt ggaaaccaac aaggcattgg atctcattcc gtcggaatat gattgggcat
     2941 tttatatcca gagtgacgaa attattcatg aaaaatatat tccggccatc agggaggcaa
     3001 tgcataggtg gaaagatgaa aggcaagtag aaggcctgtt attgaaatac ctgcatttct
     3061 acggctctta tgactatgta gggacttccc gaaaatggta ccgaaatgaa atcagaatca
     3121 taaaaaacga aaaatccatc cgttcataca aggacgctca gggattcaga aagaatggca
     3181 gcaagctcaa tgtagtcccg atcaatgcgt gggtctatca ttatggttgg gtcaaggatc
     3241 ccttggttca ggcaagaaag cacagtgatt tcaacaagct ttggcacaat gatgattatg
     3301 tagcccggat cactcaagaa atatacaact attcagatat tgatgcatta ggaagattcg
     3361 aagactctca tccggaagtc atgaaagtca gaattgcagc aaaaaactgg cattttgatg
     3421 gaacccggat cagccggaaa tccggaataa aagacaagct cctttttttc tttgaaaaag
     3481 ctacagggat t
//
LOCUS       DAOQMC010000240         3480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14267_length_3480_cov_5.046131, whole genome shotgun sequence.
ACCESSION   DAOQMC010000240 DAOQMC010000000
VERSION     DAOQMC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14267_length_3480_cov_5.046131"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKV31_08765"
     CDS             complement(<1..678)
                     /locus_tag="PKV31_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92990.1"
                     /translation="MFRTAIKFIWFDRPKSIGIIVGIVISSFLIGQQLGTLHFITNVM
                     GALIDHANHNTGEIWVVDNTTANVNNLFRLDSRLVNEIRSIPGVQHTYPIVVTSVSVT
                     LPNGRTTPFTIIGSEAPSFAGGPDTARITEGDLSALIRANSVSAEYFDSRSLKIPLNI
                     GTRLEINGKSAIIRLKTRHIQAFGGHFLYSNIDNARFYGNYPNDKVSIISVKIKPGVN
                     VRKVASDI"
     gene            complement(681..1643)
                     /locus_tag="PKV31_08770"
     CDS             complement(681..1643)
                     /locus_tag="PKV31_08770"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNB92991.1"
                     /translation="MIDSMKNFLALTILFLITSCSNKQPSRQATIASDTLSVPHNIEI
                     IVGIGKVEPLDGVIQLASSTGGIVNSVFRQEGDTVKAGEAIFTLQPENASLKIRKIKA
                     QIATERQQIVANQTAIGQYEAQLNYKTANLEAGRKLAQSGAETLQNIANQASEIDVLK
                     NQIEQGRQAVVISRSRMDELQADLRLAEHDLGSLTIRSPSAGILLSQNAQPGSAIQPL
                     QAYAYLAPRGPLVVHGEVDELFADRLALGQEATLHLMGDKQIVGSGKIIFLSPQLNNK
                     SLFTDMPGEAQDRRVRRFKIRIDPSDRPMLLNTKVECEIHLKDH"
     gene            complement(1654..2346)
                     /locus_tag="PKV31_08775"
     CDS             complement(1654..2346)
                     /locus_tag="PKV31_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB92992.1"
                     /translation="MEIVASIRRASKKYKMGDTDITALTETTLEFHSHELTLILGPSG
                     SGKTTLLSLLGCVIYPTTGDVIVMNQNIGTLKEKKLSRIRLNNIGFVFQGFNLIQPLT
                     ALENVMMPLILQGENRRQAKARAMIALEKVDMISKMNNLPHTLSGGQRQRTAIARALV
                     TNPAILLCDEPTASIDSKNASKVMEELKTLSMQGKSVIVVTHDTRLIKYADRIIYLSE
                     GRTSETPFPEEQ"
     gene            2920..3264
                     /locus_tag="PKV31_08780"
     CDS             2920..3264
                     /locus_tag="PKV31_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNB92993.1"
                     /translation="MPDSLKPCPQCNYPYAYASEGGLFSCPECGHEWNPEEIYSLEDV
                     LSVFDANGNELKNGDTVVIIKDLPVKGAPKPIKAGTKVKNIRLSEGDHNISCKIDGFG
                     AMGLKSEFVRKA"
BASE COUNT          906 a          672 c          827 g         1075 t
ORIGIN      
        1 gatatctgaa gccacctttc tgacatttac accgggtttg atttttacgg agattatact
       61 tactttgtca ttgggataat tgccataaaa gcgggcgttg tctatattcg aatataagaa
      121 gtggccacca aaagcttgta tgtgcctggt cttcaaccga atgatggcgc tcttgccgtt
      181 gatttcaagg cgggtaccta tatttaaagg tatttttagt gaacgggagt caaaatattc
      241 ggcagaaacc gagttggcgc gaatcagcgc tgacagatct ccttctgtaa tcctcgccgt
      301 gtcgggtcct ccggcaaatg aaggtgcttc actgccgatg attgtgaagg gtgtcgtgcg
      361 gccattaggg agagtgacac tgactgaagt aacgacgatg gggtaagtat gctgcacacc
      421 cggaatactt cggatttcat ttaccaggcg ggaatccaga cggaacaggt tattgacatt
      481 ggctgtggta ttgtccacga cccagatctc accggtattg tgattagcat ggtcgattaa
      541 tgcacccatc acattggtga tgaaatggag tgtgcccaac tgctggccaa tcaggaaact
      601 gcttatgacg ataccaacga tgatgcctat ggactttggc cggtcaaacc agataaattt
      661 aatggcagtg cgaaacatga ttaatggtct ttcagatgaa tctcacattc taccttggta
      721 ttgagtagca taggacggtc tgaaggatcg attctgatct tgaagcggcg tacgcgcctg
      781 tcctgtgcct cgccgggcat atcggtaaaa agagatttgt tgttgagctg aggcgagagg
      841 aatatgattt ttccgcttcc aacgatctgt ttatccccca tcaggtggag ggttgcctcc
      901 tgtccaagag ctagtctgtc cgcaaacaat tcatccacct caccatgtac cactaatggg
      961 cccctgggag ctaagtaggc ataggcctga agaggttgaa ttgccgaacc gggttgtgcg
     1021 ttctggctaa ggagaatacc ggctgaaggg gatcgtatgg ttagcgagcc cagatcatgt
     1081 tcagcaagac ggagatcagc ttgtagctca tccatcctgg agcggcttat gacgactgcc
     1141 tgtcgacctt gttcgatctg gtttttgagt acatcaattt cggaagcttg attagctatg
     1201 ttttgaagtg tttcggcacc tgattgggcc agcttccttc ctgcctccag attggcagtc
     1261 ttataattaa gttgggcttc atattgacct attgctgtct gatttgcgac gatttgttgt
     1321 cgttccgtcg caatctgggc ctttatttta cggattttaa gggaagcatt ttcgggctgc
     1381 agtgtaaata tggcctctcc ggcttttacc gtatcgcctt cctgccggaa aaccgaattg
     1441 actatacctc cagtggatga cgcgagttga atcactccat ctaagggttc tactttgccg
     1501 atgcccacaa ttatttcaat gttgtgtggt accgaaaggg tatcggacgc aatggttgcc
     1561 tgccggcttg gttgtttgtt gctacatgac gttatcaaga agagaatggt caaagcaagg
     1621 aaatttttca ttgaatctat catgataaca cgattattgt tcttctggaa atggggtttc
     1681 ggaagttctg ccttcggaca gatagataat tctgtctgca tatttgatta gacgggtgtc
     1741 gtgggtgacg acaatgaccg acttgccttg catggacaat gttttcaact cttccattac
     1801 ttttgacgcg tttttggaat caatggaagc ggtcggttcg tcgcagagca ggatggcagg
     1861 atttgtcact aaagcccgag ctatggcagt tctttgtcgc tgcccgccac tgagggtgtg
     1921 gggcaggtta ttcatttttg aaatcatatc gaccttttca agggctatca tcgctcttgc
     1981 tttcgcctgt cgccggtttt cgccctggag aatgagcggc atcattacat tttccaatgc
     2041 tgtaagtggc tggatcagat tgaagccctg gaaaacaaag ccgatgttat tgagccgaat
     2101 gcgggacaac tttttttcct ttaatgtacc aatattttga ttcatgacaa tgacatctcc
     2161 ggtcgttgga taaatgacac accccagcaa tgaaagcagt gttgttttcc cggaaccgga
     2221 cggaccaagg ataagggtca attcgtgcga atgaaattcg agcgtggtct ctgttagggc
     2281 tgtgatgtcc gtatcaccca ttttgtattt ttttgaagct cttctgatgg atgccacaat
     2341 ctccatatta aatacgattt agtattatat attgcaaata taaaatgttt tcgtattatt
     2401 taataaaact acaatggaaa atcaaaatcg gatattgaag gatgaattct ccggatggac
     2461 agaaaggttg atatggggaa attgtattgc agaaagttat tcaatcctca ttcagcaaaa
     2521 aattcctgat cttaaatcaa aaagtggtat ttgtgacatg aatacagctt ttgaagacca
     2581 agggaatacg attttatttt tttggatgta gtcatggcgc ctttattttc aagtaggtat
     2641 tattttaata aggagggaag tgtagttata gaagagatta tcagacaaaa taattaattg
     2701 atttgctgat tttctgtgtg gatgtaacaa attggacgaa aaggcttgtt gttcttaaag
     2761 tgttgtttat taattatcta cccacttaga attttattaa attttctgtc cagtgtaatc
     2821 ggactgatga tttcttttag tgttaattat ttattcctat gtttgcgtta acaaaacctt
     2881 tttcttggac tgaatttttc actttccaaa aatccaataa tgcctgatag tctgaaacct
     2941 tgcccacaat gcaactatcc atatgcctat gcttccgaag gcggactttt ttcatgtccg
     3001 gaatgtggtc acgagtggaa tccggaagaa atttactctt tggaagatgt tctaagtgtt
     3061 tttgatgcaa atgggaatga acttaaaaac ggagataccg ttgtgattat caaggattta
     3121 cctgtaaaag gagctcctaa gccaataaag gcggggacaa aagttaaaaa catccggctt
     3181 tcagaagggg accacaatat aagttgtaag attgatggat ttggggctat ggggctcaaa
     3241 tccgaatttg taagaaaagc ctgaatttat ataaaagcct atttgttttc attcactttt
     3301 tgaggctgac agcttcagta agtattaatg caggatgttc aatctaggtt ttttgcatgt
     3361 agaatgaaca gttttcagga aaccaatttc ttaccggtca tcggcctatg cctgaccatt
     3421 ctggcgatga tgtcatccag gatttcacct ttcatgcggt gcctgttgta gcggtagcag
//
LOCUS       DAOQMC010000241         3480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14268_length_3480_cov_4.999708, whole genome shotgun sequence.
ACCESSION   DAOQMC010000241 DAOQMC010000000
VERSION     DAOQMC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14268_length_3480_cov_4.999708"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKV31_08785"
     CDS             complement(<1..484)
                     /locus_tag="PKV31_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB92994.1"
                     /translation="MSNTIQESGAQQEGVAVAASMNPGNYRVCDITGFKVDLSSEKLI
                     RANAVASVISLLLAVVAALLLVFTRYQPVHLLNAEWYYRLVTFHGLNALIFWIIFFEI
                     AGLYLGSSVFLNVRFCSPKTGWVAFALMVAGLVMSNIIILMGKADVMLTSYTPLKAHP
                     L"
     gene            complement(508..1422)
                     /locus_tag="PKV31_08790"
     CDS             complement(508..1422)
                     /locus_tag="PKV31_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB92995.1"
                     /translation="MSLLSPAEGWYYKKVSKDEKMWMLIALVLCIMLFVWMVMWHVYG
                     KQNPSSTTYKTSTVEFTELTEAYTKQNMIGQDNGIPVVRPEPNSDVFLMAEMWRWSPV
                     LVLEINQAYRMHISSKDIVHGLSIQPVNMNFQVYPSYDYVLEFKPTEAGEYRVVCNEF
                     CGIGHHTMIGKIVVIEKEEDLKLYGYENFKKAATPVKTEDNKPMSEADMALMGEQVFT
                     LKGCGACHKTDGSALIGPSWKGLYGKEEKVKENGKEMTVKVDDAYIRESILEPSRKIT
                     VGFEQTMMPQMAVTDEEINQIIAYMKTL"
     gene            complement(1449..1634)
                     /locus_tag="PKV31_08795"
     CDS             complement(1449..1634)
                     /locus_tag="PKV31_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92996.1"
                     /translation="MDKKQPPEEKRVPFYRMIMDDFMLLMFLGVTIYAIFYLLWGVME
                     LSNLPAIPDALKKSLLK"
     gene            2040..2513
                     /locus_tag="PKV31_08800"
     CDS             2040..2513
                     /locus_tag="PKV31_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92997.1"
                     /translation="MSLEEQIKKIVEDHFDLLGRQDCFLVDIIQKAKKLEIYIDSDDA
                     IDFDICKGLSRVTEQFLDESLLMGDDYILEVSSPGLSRPLKLPRQFKKNIGREVTMTN
                     SDKTKTVGILTQADDENFQITRSVTRKEGKKKITEDLITSVPYTEGRKVLINIKF"
     gene            2535..>3480
                     /gene="nusA"
                     /locus_tag="PKV31_08805"
     CDS             2535..>3480
                     /gene="nusA"
                     /locus_tag="PKV31_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNB92998.1"
                     /translation="MNLTETFKDFKEGKNIDRPTMMRVLEDVFRTLIKKRYGSDENFD
                     VIVNMNTGDLELWRRRTIVPDGEVTDELTEVSITEALELDADYEVGDECNEKIDTDEF
                     GRRAVMAARQTLVSRIMELEKDEIYKRYEDRIGDLVSGEVHQILGKEVLVLDDATGQE
                     LVLPKTEMIRGDYFRKGESTRGVIKKVEMRNNLPVITLSRTEPLFLERLMEQEVPEIE
                     DGLITIKKIVRIPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDIVN
                     FTTNTILYIQRSLTPAKISDIQVSPETMKAAVYLKADQV"
BASE COUNT         1105 a          693 c          734 g          948 t
ORIGIN      
        1 agagcggatg agctttgagt ggtgtgtatg aagtgagcat cacatccgcc ttgcccatca
       61 ggataattat attggacatc accagaccgg caaccataag ggcaaacgcg acccagccag
      121 ttttgggcga acagaacctt acattgagaa aaacgcttga gccgaggtat agtcctgcaa
      181 tttcaaagaa aatgatccag aagatcaagg cattcaaccc atggaatgtg accagtctgt
      241 aataccattc agcattgagt aagtgcacgg gttggtaacg ggtaaacacc agcagcaacg
      301 cagcgacaac ggctagcagc aaggatatga cagaagcaac agcattggca cgtattaatt
      361 tttccgaact caggtcaacc ttgaagccgg tgatatcaca tactctgtaa ttaccgggat
      421 tcattgaagc tgcaacagct actccttcct gttgagctcc ggattcttgt attgtattag
      481 acataaatgc ttgattttaa atgaatatta tagggttttc atgtaggcta tgatttgatt
      541 gatctcttca tcagtgacag ccatctgagg catcatggtc tgttcaaagc caacagtaat
      601 tttcctggaa ggctccaaaa tagattcgcg gatatatgca tcatccacct tgactgtcat
      661 ttcctttcca ttttccttta ctttctcctc ctttccatat aatcctttcc aggacggtcc
      721 aataagtgct gatccatcgg tcttatggca tgctccacaa cccttaaggg taaatacctg
      781 ctcacccatc agagccatat cagcctctga cattggttta ttgtcctctg tcttaaccgg
      841 agtggcagct tttttgaaat tttcatagcc atacagcttc aaatcttctt ccttctcgat
      901 gacgactatc ttgccaatca tagtatggtg acctatgcca caaaactcat tacaaacaac
      961 ccgatactca cctgcttcgg tgggtttgaa ttccagcaca taatcatagg aaggatacac
     1021 ctgaaagttc atattaaccg gttgaatgga aaggccgtgt acaatatcct tggaagaaat
     1081 gtgcatgcgg tatgcctggt tgatttccaa gactaaaacc gggctccaac gccacatctc
     1141 cgccatcaga aatacatccg agttgggttc aggccggaca acagggattc cgttatcctg
     1201 accgatcata ttctgtttgg tataagcttc cgtaagctcg gtaaattcaa cggtactggt
     1261 cttgtaggtt gtactggatg gattctgctt accatatacg tgccacatga ccatccatac
     1321 aaaaagcatg atacagagca cgagcgctat caacatccac attttctcat ctttcgatac
     1381 ctttttataa taccaacctt cagctggact aagtaagctc ataagtagaa attttaagtt
     1441 ttacgaaatt atttcaacag ggattttttc agcgcatccg gaattgccgg aaggtttgac
     1501 aattccatta ctccccaaag caggtaaaaa atagcataaa tggtaacccc aaggaacatc
     1561 aacaacatga agtcatccat gatcatacgg tagaagggca ctcttttttc ttccggtggt
     1621 tgcttcttat ccatacattt tcgattttag gtaaattaat ttaaacatct gttaacaaat
     1681 gtattcagca ctttttcaag aaaaaaagaa tcctttggat taaatattca aaaaatacgt
     1741 tttcgtactt tttaccccgt aatgacattt actgattttt gttataagga gttaattgca
     1801 aaatattata tttaaaactt atctactgat catattatat tttgagttgt cggcaaaact
     1861 aaaaaggcct gtcagcataa gggagctaac aactcaagcg atgattggcc atagcccatt
     1921 gatactcatt gcaatagtta aataaaaaat ttggcggttt gataattaat gcttaccttt
     1981 gtgtcagatt gcaagcaatt ggtagggagc ggaagttccc tattttattt tatataacaa
     2041 tgtcattgga agagcagata aaaaaaatag tagaagatca tttcgatcta ttggggcggc
     2101 aggattgctt cttagtcgat attatacaaa aggctaaaaa actggaaata tatattgact
     2161 cggatgatgc cattgacttt gacatctgta aaggtctgag cagggtgacc gaacaatttc
     2221 ttgatgaaag cttattgatg ggagatgact acattttgga ggtgtcttcg ccagggctta
     2281 gcagaccatt gaagttaccc cgtcagttta agaaaaatat aggaagagag gtcactatga
     2341 ccaattccga taagacaaaa acagtcggga tattgacaca agcggatgat gaaaattttc
     2401 agattaccag aagtgtcacc cgaaaagagg gtaaaaaaaa gattacagaa gacctgatta
     2461 cctcagtgcc ttataccgaa ggacgaaaag tactgatcaa cattaaattt taaaccatat
     2521 tataattttc aaaaatgaac ttaacagaaa cctttaagga tttcaaggaa ggtaaaaata
     2581 ttgaccgccc taccatgatg cgtgtattgg aggatgtatt ccgcacactt atcaaaaaaa
     2641 gatatggttc agatgaaaac ttcgacgtca tagtaaatat gaataccgga gaccttgagc
     2701 tttggagaag gaggactatt gtccctgatg gtgaagtgac ggacgaattg acagaagtat
     2761 ccatcacaga agcgctcgaa ttggatgcag attatgaggt aggtgatgag tgcaacgaaa
     2821 aaatagatac cgatgaattc ggaaggcgtg cagtcatggc agcaagacaa acactggtat
     2881 cccgtatcat ggaactggaa aaggatgaaa tatataagcg ctacgaagat agaataggcg
     2941 atctggtgtc aggtgaagtt catcagattc tgggcaagga agtccttgtc ctggatgacg
     3001 ccacggggca ggaattggta ctcccaaaga cagaaatgat ccgtggggac tattttcgca
     3061 aaggggagtc aacacgcggt gtgatcaaga aagtcgaaat gcgcaacaac ctccctgtca
     3121 ttaccttgtc aagaacagaa ccattatttc tggaaagact aatggaacag gaagtaccgg
     3181 aaattgaaga cggactgatt accatcaaga agattgtgag aattcccggt gaaagggcta
     3241 aagtcgcagt cgaatcctat gacgaccgaa tcgatccggt cggtgcctgt gtggggatga
     3301 agggaagccg tatccatggt atagttaggg aattgagaaa cgaaaacatt gatatcgtca
     3361 atttcactac caacaccata ttgtacattc aaaggtcact tacacctgcg aagatttctg
     3421 atattcaagt atcaccggaa accatgaaag cagctgtata tcttaaagcg gatcaggttt
//
LOCUS       DAOQMC010000242         3478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14285_length_3478_cov_4.397604, whole genome shotgun sequence.
ACCESSION   DAOQMC010000242 DAOQMC010000000
VERSION     DAOQMC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14285_length_3478_cov_4.397604"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PKV31_08810"
     CDS             <1..1078
                     /locus_tag="PKV31_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB92999.1"
                     /translation="ERKSFVSSTFTIFLGLAFASSLVLIAGRPLLLPFLIESRTIDGF
                     YWFCLYSMLNIPSMLIPSLMMLQGNSRELLRFSMFYAIGYTMVFVLNMVFGGRLINLL
                     ILLNVFSFALLVMSGAMSLSLDLRAYRLKWVKRLFLIGSPLVGYAILNGLAPLFDSWL
                     VQRFYTDKAVFAIYKYGAREFPLTVTLAIGLGMALVPMLGRNPTSGMEQIKQRSRKLF
                     PIVFITSALLILTSKYFFPIVFNNNFTSAAAVFNLYVLIIISRMIYPHTIMMGLKRTK
                     ALLYISITEIFIHITSSLLLGLKFGLVGIAGGAVIAFTIERLLQVVYLKWKRGINVNQ
                     YVQLKPYILYSLIVIVAWLLTIYL"
     gene            complement(1170..1252)
                     /locus_tag="PKV31_08815"
     tRNA            complement(1170..1252)
                     /locus_tag="PKV31_08815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1217..1219),aa:Leu,seq:taa)
     gene            complement(1279..1354)
                     /locus_tag="PKV31_08820"
     tRNA            complement(1279..1354)
                     /locus_tag="PKV31_08820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Gly,seq:gcc)
     gene            complement(1439..2540)
                     /gene="prfB"
                     /locus_tag="PKV31_08825"
     CDS             complement(join(1439..2479,2481..2540))
                     /gene="prfB"
                     /locus_tag="PKV31_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_090170511.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNB93000.1"
                     /translation="MTIEQLKDLQDRLASLRRYLDIDKRLHEIEDKTNESLATDFWSN
                     PEKAEATLKELKSHQTWVNKFRSGELSVEEAAVALELAKEDAMTEAEVDEVYRKSMEI
                     IDDMEFKSTITKPEDELNAILEINAGAGGTEACDWASMLMRMYIMYAEKNGFKVTELS
                     STDGDVAGIKSVTLEIVGDLAYGLLKGENGVHRLVRVSPYNAQGKRMTSFASVFVHPM
                     VDDRIEVIINPADLEWDTFRASGAGGQHVNRTESAVRVRHLPTGIVAECQQERSQIQN
                     REKAMMLLKSRIYELELEKQRAEKDAVEASKMKNEWGSQIRSYVLDDRRVKDHRTGYT
                     NNNTDAVLNGDISGFLKEFLLWDGSSASGNSD"
     gene            complement(2591..3427)
                     /locus_tag="PKV31_08830"
     CDS             complement(2591..3427)
                     /locus_tag="PKV31_08830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB93001.1"
                     /translation="MTLPGLVMSQSRKKRNKTPQPVALTAPGGYQYDIFGSGSQLKVE
                     EGDLIYFNLQICHRDSVIEDSKKSIQVPEYVMPGKDLVVNDGPVASGLSVMALGDSLI
                     IYERIDTIAGLPETMKDWKEIIYKIKVIGVTKKSELEEVKLRIPAIQAIIEKDIDDYY
                     NQRFQKTLHTQSGVTVLVHEDGNGERFKPGEVTFVSFYAVSTKDRKYYGSTFESGRPF
                     QVRLGYKSVIEGLDDALSMPKRGAVVTAFIPARLAYGAEGVPGALEPNTDMTFYIEVL
                     DK"
BASE COUNT          956 a          784 c          624 g         1114 t
ORIGIN      
        1 tgagagaaaa tcattcgtca gttctacttt tactatattt ctcgggcttg cctttgcaag
       61 ttcactggta ttaattgccg ggaggccttt actgctaccg tttttgattg aatcacggac
      121 aatcgatgga ttttactggt tttgccttta cagcatgctc aacataccat ccatgttaat
      181 tccctctcta atgatgttgc aagggaacag tcgggagttg ttgcgctttt cgatgtttta
      241 tgcaataggc tacaccatgg tattcgtatt gaatatggta tttggtggta ggcttatcaa
      301 cttactcata ttgctcaatg tattttcctt cgccttgctg gtcatgtccg gggcaatgtc
      361 actgagtctt gacttacgag catataggct aaaatgggta aaaaggttgt tcctgatagg
      421 aagtcctttg gttggatatg caattctgaa tggtctggct ccactttttg attcctggtt
      481 ggtacaacga ttctataccg acaaagcagt tttcgcaata tataaatatg gtgcgcgtga
      541 atttcctctg acggtaacac tggcgattgg attaggaatg gcacttgttc ccatgctggg
      601 gagaaatccg acatcaggga tggaacagat caaacaacgc tcaagaaaat tattccctat
      661 cgtatttata acttcagctc tacttatcct cacttcaaag tatttctttc cgatcgtatt
      721 caataataac tttacttcag cagctgccgt atttaatcta tacgtactaa tcatcatcag
      781 tcgcatgata tacccccata ccatcatgat gggattaaag cggaccaaag cattattata
      841 tatttcgatt accgaaatct tcatacatat tacgtccagt ttgttattag gcttaaaatt
      901 tggcttagtt ggaattgcag gtggagctgt tatagcattt acgatagaga gactactgca
      961 agtcgtctat ttgaaatgga aaagaggcat caatgtcaat cagtatgtgc aattaaaacc
     1021 ctacatcctg tattcattaa ttgtaattgt cgcctggttg ttgacgatat atctatagga
     1081 aaaagatata gacccaaaaa tctttttaaa aaggatttag aaattcaaat tgcaatgttc
     1141 taccttaagg ctatgtgggt gaaaaagtgt acccggagcg ggacttgaac ccgcacgact
     1201 tcgcagtcaa aggattttaa gtccttcgtg tctaccattc caccacccgg gctaaagtat
     1261 aatagaggaa agtaaaaatg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg
     1321 caaggtcgtg ctctaccaac tgagctattc ccgcataaaa attgcttttc ttcagaagcg
     1381 tggcaaaggt atattttaat ttttaattgt aaaaattatt taatcaattt ttttttaatt
     1441 aatctgaatt gccagacgct gagctcccat cccataaaag gaattccttc agaaatcctg
     1501 atatgtcacc attcaatact gcatccgtgt tattgttggt atatccggtt cgatgatctt
     1561 ttactcgtcg gtcatccaat acatatgaac gaatctgact cccccattca tttttcattt
     1621 tggaagcctc tactgcatcc ttttctgcac gttgcttttc taattcgagt tcatatatcc
     1681 tggatttcaa caacatcata gccttctctc tattctggat ctggcttctt tcctgttggc
     1741 attccgcaac aatgccagtt ggtaagtgtc ttacacgcac agctgattct gtcctgttaa
     1801 catgctgacc accagctccg cttgcgcgaa atgtatccca ttcaaggtca gcagggttga
     1861 taatcacttc aattctatca tctaccatcg gatgaacaaa gaccgatgca aaggatgtca
     1921 ttcttttgcc ttgtgcattg tagggactaa cacgaaccaa tcggtgtaca ccattctcgc
     1981 ctttcaaaag gccatatgca agatctccga ctatttcaag ggtcactgac ttaattccgg
     2041 caacatcgcc gtcagtactt gataactcgg ttactttaaa gccgttcttc tctgcataca
     2101 taatatacat gcgcattagc attgaagccc agtcacaagc ttcagttcca cctgcacctg
     2161 cattgatctc aaggatagca ttgagttcat cttcaggttt agtaatggta gacttgaatt
     2221 ccatatcatc gatgatctcc atcgactttc tgtacacctc atccacttcc gcttctgtca
     2281 tagcgtcttc cttagctaat tcaagagcta cggctgcttc ttcgacggat aattcgccac
     2341 tcctgaattt atttacccat gtctgatggg attttaattc cttaagtgtc gcctcggctt
     2401 tttccggatt gctccagaag tctgtagcga gggattcatt ggttttatct tctatctcat
     2461 gtaatcgctt atcgatgtca aagatacctc ctaagagaag ccaacctgtc ctgcaggtct
     2521 ttaagctgtt cgatagtcat atcgtttaat tttattaaat aaaaaattaa ttattcacag
     2581 aaaacaaaaa ctatttatca agaacctcaa tatagaaagt catatccgta ttcggttcca
     2641 atgctcctgg aacaccttct gccccatatg ccaatcttgc aggaatgaaa gctgtaacca
     2701 cagcacctcg ttttggcatg gacaacgcat catccaaccc ctcaatcact gatttatagc
     2761 caagtctgac ctggaaaggg cgcccggatt caaaagtact cccatagtat ttccgatctt
     2821 tagttgatac ggcataaaag cttacaaaag taacttcgcc cggtttaaaa cgctctccat
     2881 ttccgtcttc atgtaccaat acagttactc cactttgtgt atgaagagtc ttttgaaacc
     2941 tttgattgta ataatcatca atatcctttt cgataatagc ctgaatagca ggtatccgca
     3001 atttgacctc ttccagttct gactttttag ttaccccgat taccttaatc ttataaataa
     3061 tttctttcca atccttcata gtctcaggca aaccggcaat agtgtctatc ctctcgtata
     3121 tgatgaggct gtcgccaaga gccattacac ttagccctga agcaacagga ccatcattta
     3181 ccaccaaatc ctttcccggc attacatact cgggcacctg aatagatttt tttgaatcct
     3241 cgatcactga atcacgatgg catatctgca aattaaaata tatgagatct ccttcctcaa
     3301 ctttcaattg gctcccgcta ccgaaaatat catattgata tccaccggga gcggtcaatg
     3361 caactggttg gggagtctta tttcgtttct tcctgctttg tgacattact aatcctggta
     3421 atgtcaaact gataagaatt aaaaataata ccctcatttg atttcgttaa attgcatc
//
LOCUS       DAOQMC010000243         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14524_length_3444_cov_5.421068, whole genome shotgun sequence.
ACCESSION   DAOQMC010000243 DAOQMC010000000
VERSION     DAOQMC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14524_length_3444_cov_5.421068"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKV31_08835"
     CDS             <1..784
                     /locus_tag="PKV31_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNB93002.1"
                     /translation="KSMFNVDTDIIGYFIFGSMIIVPAIIISDKSLTSTEKSRIWVIF
                     ILAFFVIFFWGAFEQAGASLTIFADKQTDRHIFGWEMPASYFQSVNPLSIIVLAPLFS
                     AMWIKLGNRNMEPATPRKMALGLSLVALGYVLIAFAVKGVDASTKINMFWLFSLYVVH
                     TMGELCLSPIGLSMVAKLSPARFSSLLMGTWFLANAAANKFAGTLSELIPSESNPTTS
                     FVGFQITNLYEFFLVFIIMCGVAAGILFLISGKLQKMMGGIK"
     gene            857..2368
                     /locus_tag="PKV31_08840"
     CDS             857..2368
                     /locus_tag="PKV31_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNB93003.1"
                     /translation="MLTIDQVRDFKGKYPRQIWSLFFSEMWERFCFYGMRGMLVFFMT
                     RQLKFGDIQANLQYGATQAFVYAFTFVGGLFADKILGFRKSLYWGGFMMIIGSSLLAV
                     DPHQFFFLGLSFIIIGTGFFKPNISTMVGELYKDGDDRRDAGFSLFYAGINLGAFLGG
                     YFCVAIGKGNMFSGMIDEAHRWNVAFGLAAVGMIISLINFFYTQKSLGPIGLQPGHPH
                     ALVDTRAPGRWTEYAVYIGTLLLIPIIQIMVSKPEYTDYFMYTIGPVTLIYLFYEMSK
                     VTDKERKKLWAALIFIFFSILFWGIYEQSGGSLSIFAAKNLNDSLLGMKFDPNGVNNS
                     AGAFFIIALAPLFGLMWLWMAKRGFEPNTIIKFGLGFILLGLGYYTLFTTRFFATNGV
                     SSLDIFTIALLIITFGELCLSPIGLSIMTKLSPARLQGIMMGMWFLASAYGQYVAGLI
                     GASLAQAEADASATVTMEAYTDGYKMLGFYALIAGAILIAASPWIKKLMQGVK"
     gene            2373..>3444
                     /locus_tag="PKV31_08845"
     CDS             2373..>3444
                     /locus_tag="PKV31_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNB93004.1"
                     /translation="MKNLVRFLLPFFIIITWNMPGMADNGDPVRINPTNWWIGMEDNT
                     VQILIQGHSDGFNNQAVSINYPGVKLEKIHRLPNKHYFALDVSISPATKPGVVRIHIG
                     KDSYDWSLAMKNVKDNGKSYAQGVTAKDLIYLIMPDRFSNGDLSNDRIPGMKDQSLNR
                     DTVFNRHGGDLQGIINHLDYFNQLGVTALWLNPVLENDMPERTEHGYAITDHYRVDPR
                     LGGNEAYTKLVEEAHRKGIKILQDAIYNHIGLDHWSVTDPPSPDWLHHWEHYTQTTYK
                     DQVLMDPYASIADRKRMSDGWFVPSMPDLNQNNPYVANFLIQHAIWCVEQFGIDGWRI
                     DTYAYNDLGFMNRCNAALIREYP"
BASE COUNT          845 a          719 c          828 g         1052 t
ORIGIN      
        1 caagagcatg ttcaatgtgg atactgatat cataggatat tttatctttg gctcgatgat
       61 catcgtacct gctatcatta taagtgataa gtcgcttaca tcaacggaga aaagcaggat
      121 ctgggtaatt tttatccttg cgttttttgt gattttcttt tggggagcgt ttgagcaggc
      181 aggtgcgtca ctcacaatct ttgccgataa gcagaccgat cgtcatatat ttggctggga
      241 gatgccggca agttactttc agtcagtcaa tccgctttcc atcattgtac tcgctccttt
      301 gttctccgct atgtggatta agcttggcaa caggaatatg gagccggcca caccacgtaa
      361 aatggcattg ggcctttccc tggttgcctt aggctatgtc ctaatcgcct ttgcggtcaa
      421 aggtgttgat gcgagtacaa agatcaatat gttttggttg ttcagcctat atgtggttca
      481 taccatgggt gaattgtgtt tgtcgccgat aggactttct atggtcgcca aactttcacc
      541 ggccagattc tcttctttgt tgatgggaac ctggttcctt gccaatgctg cagccaataa
      601 atttgccggt acgttgagcg aattgattcc gagtgagagc aatccaacca cttcatttgt
      661 tggtttccag atcaccaatt tgtatgaatt cttccttgtt ttcattatta tgtgtggtgt
      721 cgctgccggt atattgttcc tgatcagtgg caaattgcaa aaaatgatgg gcgggattaa
      781 ataaggttct tttaaaataa aatgattaat ttgcaaggac gttttactta aggcgtcctt
      841 tttaatttct tgcgatatgt tgacaattga tcaggttcgt gacttcaaag gcaaataccc
      901 acgccaaatc tggagcttgt ttttctctga gatgtgggag cgtttctgct tttacgggat
      961 gcgcggtatg ttggtatttt tcatgacccg ccaactcaaa ttcggagata tccaggccaa
     1021 tcttcagtat ggtgccacac aggcctttgt ttatgccttt acctttgtag gcggtctctt
     1081 tgccgataaa attctcggat tcaggaaatc cctatactgg ggcggattca tgatgattat
     1141 cggaagctcc ttgctggctg tcgatccgca tcagtttttc ttccttgggc tttccttcat
     1201 tattatcggc accggcttct ttaagcctaa tatctctacc atggtaggtg agttgtataa
     1261 agatggcgat gataggcggg atgccggatt ctccttgttt tatgccggta tcaatctggg
     1321 tgctttcctg ggtgggtact tctgtgttgc cataggcaaa ggtaacatgt tcagtgggat
     1381 gatcgatgag gctcatcgat ggaatgttgc ttttggactg gctgcggtgg gaatgatcat
     1441 cagtttgatc aatttctttt atacgcaaaa aagcctggga ccaatcggcc ttcaacccgg
     1501 acatccacat gcacttgtag acaccagggc tccgggccga tggaccgaat atgctgtcta
     1561 tattggtact cttctgttaa tccccattat ccagatcatg gtatcaaaac cggaatatac
     1621 cgattatttt atgtacacca tcgggcctgt aacgttgatc tacttgttct atgagatgtc
     1681 aaaggtcacg gacaaggaac gtaagaaatt gtgggcggct ttgatattca ttttcttttc
     1741 gattctcttt tggggaatct atgaacagag tggtggttca ctcagtattt ttgctgccaa
     1801 aaacctcaac gattccttgt tgggtatgaa gtttgatccc aatggtgtca acaattcggc
     1861 gggtgctttc tttatcattg ccctggcacc gcttttcggt ctcatgtggc tttggatggc
     1921 caagagggga tttgaaccga atacgatcat caagtttgga ttgggcttta tcctcctcgg
     1981 actgggctac tataccctgt ttacaacacg gttttttgct acgaatggag taagctcact
     2041 ggatatattt accatcgctt tgctgattat tacatttgga gagctttgcc tttctcccat
     2101 aggtttgtcc atcatgacca agctgtctcc ggccagattg cagggaatca tgatgggtat
     2161 gtggtttctc gcttctgcat acggtcagta tgtagccgga ttgatcgggg caagccttgc
     2221 ccaggcggag gccgacgctt cagcgacagt tactatggaa gcttataccg atggttataa
     2281 aatgctggga ttctatgcat tgatagccgg cgccatcctt attgctgcat cgccgtggat
     2341 taaaaagctt atgcagggag ttaaataata ctatgaagaa tttagtaaga tttcttttgc
     2401 cgtttttcat catcattacc tggaatatgc cgggtatggc agataatggc gatcctgtgc
     2461 gtattaaccc gaccaactgg tggatcggta tggaagataa cacagtgcag atattgattc
     2521 agggacatag tgatggcttt aataatcagg cggtttctat caattatccg ggagtgaagc
     2581 tggagaaaat ccaccgactt ccgaataaac actactttgc actggatgtt tccatatcgc
     2641 cagctacaaa gcccggagta gtcaggatcc atatcgggaa ggattcctat gactggtcac
     2701 ttgctatgaa aaatgtaaag gacaatggaa agtcctatgc acagggagtg actgcaaagg
     2761 atctaatata tcttatcatg ccggatcggt tttccaatgg tgatctctcc aatgaccgta
     2821 ttcccggtat gaaggatcag agtctcaacc gcgatacggt gttcaacagg catggtggag
     2881 atcttcaagg gataattaac catctggatt attttaatca gcttggtgtg acggccttat
     2941 ggctaaatcc tgtattggag aatgatatgc ctgagcggac agaacacggt tatgccatca
     3001 ccgatcacta tcgggtagac ccacgtctgg gtggtaatga agcttataca aagctagtcg
     3061 aagaagcgca caggaaaggt atcaaaatcc tccaggatgc gatatacaat catataggac
     3121 tggatcactg gagcgttacc gatccgccgt caccggactg gctgcatcat tgggagcatt
     3181 atactcagac tacctataag gatcaggtcc taatggatcc ctacgcaagt atcgctgacc
     3241 ggaaaagaat gagcgatgga tggtttgtgc cttccatgcc agatctgaat caaaataatc
     3301 catacgttgc caacttcctg atacaacatg ctatctggtg tgtggaacag ttcggtattg
     3361 acggttggcg aatcgataca tatgcttaca atgacctcgg gtttatgaac cggtgtaatg
     3421 ccgccttgat cagggaatac ccgt
//
LOCUS       DAOQMC010000244         3440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14561_length_3440_cov_3.521713, whole genome shotgun sequence.
ACCESSION   DAOQMC010000244 DAOQMC010000000
VERSION     DAOQMC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14561_length_3440_cov_3.521713"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..961)
                     /locus_tag="PKV31_08850"
     CDS             complement(164..961)
                     /locus_tag="PKV31_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93005.1"
                     /translation="MNKKSLKLSLNILGLLLFIVAVLGLISSTEKESTGNVKKVIVDI
                     KPLDGGVLLVNNEDLARLLKKDYDLKLKGQEIGLLDLGKIESIIEHNEFVQDAQVYID
                     ARNNLKIRVIQNNPILRVISPGQINFLITDQGEKVPMPDNAVLRLPVLTGSLSSFTVK
                     AMKDSSDVYFKAYSIAMAMKKNPFMNSLAEQLVADGNGDIILIPKLGNHKIILGDAQN
                     LSIKFKKLEIFYKNAMPAEGWNMYKEINLKYKDQVIAKKAETIPIKS"
     gene            complement(958..2328)
                     /gene="murC"
                     /locus_tag="PKV31_08855"
     CDS             complement(958..2328)
                     /gene="murC"
                     /locus_tag="PKV31_08855"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNB93006.1"
                     /translation="MKEMKGIRKIFFLGIGGIGMSALAKYFHRRGIEISGYDRMHTPL
                     IAELESMGMKITHLDQAIGLDRPDLVIYTPAVPSTSILRNYFESQGIPMMKRSQVLGF
                     ISDSEKTIAIAGTHGKTTTTAMATHILYHSGVKVTGFVGGIMADYNSNYVDDGSDYVV
                     VEADEFDRSFLTLTPYISAVISMDPDHLDIYGSQHEVLRGFKDFINKTIPGGTVYLHR
                     STVSKFTADEFYVLQRTYEILTFGDSTSDIYADNIRFEEDGTTKFDFHYKTQLFSDVT
                     INMPGRHNVSNATVAMSIGLQLKLSPKVVFEAISGFKGIKRRFEIIVKTPDCIYIDDY
                     AHHPEELRNAIATAKSIYPDQKITGIFQPHLFSRTRDFMAEFAEVLDELDEPILLDIY
                     PARELPIEGITSAALLSKMSNPNKRLMDFDSVCEYISITKPKVLITLGAGDIDTIVPK
                     LKKILS"
     gene            complement(2325..3431)
                     /gene="murG"
                     /locus_tag="PKV31_08860"
     CDS             complement(2325..3431)
                     /gene="murG"
                     /locus_tag="PKV31_08860"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNB93007.1"
                     /translation="MKRVIISGGGTGGHIYPAIAIADAVKAALDNVELLFVGAEGKME
                     MEKVPKAGYRIIGLPVAGFQRRLTLKNLMFPFKLIRSMARSKKVVRDFKPDIAIGVGG
                     YASGPVLRVASGSAIPTVIQEQNSYPGVTNKILAKRASAICVAYEGMERFFPADKIHL
                     TGNPVRRDIADLKDKRDEGIRFYHLDPAKKTILVMGGSLGARTLNEALAAATQMIAGN
                     EDIQWIWQSGSFYKGMYDDSPLAKLSNVRNLAFIERMDLAYACADLVIGRAGALTISE
                     ICIAGKPSILVPSPNVAEDHQTKNAMALVEKDATILVKDDDAMNELVSQSIALVRNEE
                     RMRLLGAHARSMGKPNAADDIARVIIEILNRNAL"
BASE COUNT          932 a          825 c          642 g         1041 t
ORIGIN      
        1 acctttccga cggccaatat ctccaggcgg ccaaaaaggt cccgacgacc ggccattgca
       61 cacactttgg tggtgccaat gtcaacggcg actacaatcg gattctcatt tgtgaaattt
      121 ttgtccatag taataagatt tcaaaaatta taattaggtt gttttaactt tttatgggta
      181 tagtttcagc tttctttgca atgacctgat ccttatactt cagattgatt tccttgtaca
      241 tattccagcc ttctgccggc atagcattct tatagaaaat ttccagtttt ttgaatttta
      301 tactcaggtt ttgtgcatct cccaaaataa ttttgtgatt tcctagcttc ggaataagga
      361 ttatgtctcc atttccatca gcgacgagtt gttcagcaag gctattcata aaagggttct
      421 tcttcattgc catcgctatg ctgtaagctt tgaaatacac atcactggaa tccttcatag
      481 cctttactgt aaacgatgat aaggatccag taagaaccgg gaggcgtaaa acagcattat
      541 ccggcatagg gaccttctcc ccttgatcgg ttatcaggaa attgatttga cccggactga
      601 ttacacgcag gattgggttg ttctgaatta ccctgatctt caaattattt cgtgcatcga
      661 tatagacctg cgcatcctga acaaattcat tatgctctat gattgattcg atcttgccca
      721 ggtcaagaag ccctatttcc tgacctttca atttcaggtc ataatctttc ttaagcaatc
      781 gtgccagatc ttcattgttg accaaaagca cacctccatc caaaggtttg atgtctacaa
      841 taaccttttt aacattaccc gtgctttcct tttcagtgga gctaatcaag cccaacactg
      901 caactatgaa gagtagcaaa cccagaatat tcaaactcaa tttcaatgac ttcttgttca
      961 tgacaatatt tttttcaatt taggaacaat ggtgtcaata tctccggcac ccagtgtaat
     1021 cagaacctta ggctttgtaa ttgaaatata ttcacatact gaatcaaaat ccatcaaccg
     1081 tttattggga ttgctcatct tgctcaacaa ggctgcactg gtaatccctt cgataggcaa
     1141 ttcgcgtgca ggataaatat caagcaaaat aggttcatct aattcatcca atacctcggc
     1201 aaattctgcc ataaaatctc tggtcctgga aaaaagatga ggctggaaga ttccggtaat
     1261 cttttggtca ggataaatac tcttcgcagt ggctattgca ttcctcaatt cttccggatg
     1321 atgtgcataa tcatcaatat agatgcaatc aggtgtctta acaataatct caaacctgcg
     1381 cttaattccc ttgaaaccac tgattgcttc aaacactact ttgggtgaga gtttcagttg
     1441 gaggccgatg gacatggcta ctgttgcatt ggatacattg tgccttcccg gcatattgat
     1501 agtaacatcg gagaagagtt gggtcttgta atgaaaatca aacttggtcg ttccatcctc
     1561 ttcaaatctg atattatccg catagatatc ggaagtagaa tctccaaaag tcaatatttc
     1621 ataagttctc tggagaacat agaattcatc ggcagtaaat ttggaaactg tagatcggtg
     1681 cagatataca gtccctcccg gtatagtctt gttgataaaa tccttaaaac ccctgagtac
     1741 ttcgtgctgg ctgccataga tatccagatg atcgggatcc atgctaatta cagcactgat
     1801 ataaggcgta agtgtcaaaa aggatctgtc gaattcgtcg gcttccacta caacataatc
     1861 agaaccatca tctacataat tggaattata atctgccatg atgccaccaa caaatccggt
     1921 aaccttgacc ccactatgat ataaaatgtg agtcgccata gcggtagttg tggtctttcc
     1981 atgagtcccg gctattgcta tggttttttc cgaatcactg ataaatccaa gcacctgaga
     2041 tcgcttcatc atgggtattc cctgcgattc aaaataattg cgtaaaattg atgtcgaagg
     2101 cacagccggt gtatatatga caagatccgg tctatcaagg ccaatcgcct ggtcgaggtg
     2161 ggtaatcttc atacccatgg attctaactc ggcgatcaat ggagtgtgca ttcgatcata
     2221 accggagatt tcaatgcccc gcctatggaa atatttcgcc agggcgctca tccctattcc
     2281 acctattccg agaaagaata tttttcttat gcctttcatc tccttcataa agcattcctg
     2341 tttaatattt caatgatgac ccgtgcaata tcatcagcag cattcggttt tcccatcgat
     2401 cttgcatgtg cgcccaatag ccgcattctt tcttcatttc tgacaagtgc tatgctttgg
     2461 ctgaccaatt cattcatcgc atcatcatcc ttcaccagga ttgtcgcatc tttctcgacc
     2521 aatgccatgg cattcttagt ctgatgatct tctgctacat taggtgatgg cacaagaatt
     2581 gatggtttcc ctgcaataca aatttccgat atggtaagtg cccctgccct tccaatcacc
     2641 aaatctgcgc atgcgtaagc cagatccatc ctttctatga atgccagatt tctgacattt
     2701 gacaacttcg ccaaggggct gtcatcgtac atacctttat aaaaactacc actctgccaa
     2761 atccattgaa tatcctcatt tccagctatc atttgagtcg ctgctgcaag agcttcattg
     2821 agcgtccgcg ccccaaggct tccacccatc actaaaatgg tttttttagc aggatccaaa
     2881 tgataaaaac ggatgccttc gtctctttta tccttgaggt cggcaatgtc ccgacgaaca
     2941 ggatttccgg tgaggtgaat cttatctgcc ggaaaaaatc tttccatccc ttcataagct
     3001 acacagatgg cagaagccct ctttgccaga attttattgg tgactcccgg ataactgttc
     3061 tgctcttgga ttacggtagg gatagcgcta ccggaagcta cccgaagtac agggccgctt
     3121 gcatatccgc ccacaccaat agcgatgtcg ggcttgaaat ctctgacgac ctttttcgac
     3181 ctggccatac ttcgaatcaa tttaaaaggg aacatcaggt tcttcaaggt aagcctacgc
     3241 tgaaagcctg caaccggaag accaataatt ctatagcctg ctttgggtac tttttccatc
     3301 tccattttac cttcagcgcc tacaaacaac aattctacat tgtccagggc agccttcacc
     3361 gcatccgcaa tggcgattgc cgggtaaata tggcctcctg tgcctcctcc gctgataatc
     3421 accctcttca tccttctgtt
//
LOCUS       DAOQMC010000245         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14844_length_3403_cov_4.755376, whole genome shotgun sequence.
ACCESSION   DAOQMC010000245 DAOQMC010000000
VERSION     DAOQMC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14844_length_3403_cov_4.755376"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..575)
                     /locus_tag="PKV31_08865"
     CDS             complement(222..575)
                     /locus_tag="PKV31_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNB93008.1"
                     /translation="MSKNKKNRDGVVYSTDPDYNYNYFSDLQPVEQGFDPAMMKLRVA
                     IDRKQRGGKEVTLITGFRGNESELNDLAKKLKTKCRVGGSAKDGEIIIQGNHKEKVIN
                     MLHEWGYKETKGSGG"
     gene            complement(577..1629)
                     /locus_tag="PKV31_08870"
     CDS             complement(577..1629)
                     /locus_tag="PKV31_08870"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNB93009.1"
                     /translation="MASLPGRAQRLHLENEIRKIVRLDDDIDLNQGSCTVIGIIDGDS
                     TYTYQWGTLEHQSQSPVTDTSLFVAGGVSHAQLILLTYHMMQLGLINIDDSLGRFQFR
                     EFSGSRLNTFTIGSLLSHNTGLPAILPELTHLKADPDDDYKDYTETAIDSALVVRTKE
                     SGPEGNRPFSHYNQYLLSKVLWAVKDKRVHDNGRMIPEMSHTWFLPLDALKAGSIPAH
                     YTAQQRMLSMPHFNALTPAMGMVTCMDDLVIMTKVLLRLSVQSDFQKTFLDARTTRTK
                     KGIPLSKGGFRMIDGHKSGNIYFMTGLLKGESAFLCFAPKTHTAVIILHNAGVSQHIL
                     GHQIMRMINYQYTRTY"
     gene            complement(1675..>3403)
                     /gene="hscA"
                     /locus_tag="PKV31_08875"
     CDS             complement(1675..>3403)
                     /gene="hscA"
                     /locus_tag="PKV31_08875"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNB93010.1"
                     /translation="LVLHHKNSTSLVPSVIYLDENHNAIVGSLAKEKLVSDPASTIFS
                     VKRLMGRSFKDVEAHRNFFSYEIVDTDTESLVKIKSGNKFYTPIELSALILKELKRIA
                     EEELGRKVSECVITVPAYFNDSQRQATKDAGKLAGWDVLRIINEPTAAALAYGFDKTS
                     EEGRNIAVYDMGGGTFDLSILTLEGGVFEVLSTKGDTWLGGDDVDKAILDFVIKKGIN
                     NVDLDKGQMQQLRLVCETAKKYLADHDIFEGSFDNQQIILTREEYKNLTMPFIKKSLE
                     LTQAALKDAGLDAKDIAEVILVGGSTRIPFVKESLSGIFSAPINDKINPDEVVAQGAA
                     IQADILAGNQSHLLLLDVTPLSLGIETVGGLMDVIIPRNSKIPSGAGRHYTTSVDGQV
                     NLRISVFQGERDLVKDNRKLGEFTLRGIPPMPAGLPKIEVVFKVDADGILLVKAREER
                     SNTETQVEIKAQYGISEEEMALMLLDSIKNAKEDVKIKALIEARNEANNIILSTEKFL
                     NQHAELFTDEEFAPLILIADKLKEVVKGEDKDAIHDIIHQLDKIARPMAEKAMDYSIR
                     QSLAGKSIV"
BASE COUNT          843 a          767 c          740 g         1053 t
ORIGIN      
        1 aaggaatcag catcggaaaa gaatggtgcc attgcgatca tgatcgagaa gccatctgtc
       61 atcaaacgcc cattgataga ggatgaaagc ggaaaagtga tgtgtctggg cttcagtgat
      121 gagttgtatg aggacttgtt caatggcaaa tgattgcctg gcaattttaa ggaataatga
      181 ccatttaggt tttttgggac ctttaaatgg gaagccattt cttatcctcc gctgcccttg
      241 gtttccttgt agccccattc atgtagcata ttgataactt tttccttgtg atttccttga
      301 atgattatct cgccatcctt ggcacttccg cctactctgc actttgtctt cagtttcttt
      361 gccaaatcat tgagctctga ctcatttccc ctgaatcctg taatcagggt gacctccttt
      421 ccacccctct gctttcggtc gattgccacc cgtaacttca tcatggcggg atcgaatccc
      481 tgttccactg gttgtaagtc ggaaaaataa ttgtagttgt aatcaggatc tgtggaatag
      541 acgactccgt ctctgttttt tttgttttta gacatatcag taagttctgg tatattgata
      601 attgatcatg cgcatgattt gatgtccgag gatgtgctgt gacacgccgg cattatgaag
      661 gatgattact gctgtatgcg ttttgggagc aaagcaaaga aatgcacttt cgcctttgag
      721 taatccggtc atgaaataaa tgttgccgga tttatgtcca tcaatcatcc tgaatccgcc
      781 cttactcaat ggtatcccct tctttgtcct tgtagtgcgg gcatcgagaa aggttttttg
      841 aaagtcagat tggacggata acctcagcaa cacttttgtc ataatcacca ggtcatccat
      901 acacgtcacc atacccatag cgggtgtcaa tgcattgaaa tgcggcattg aaagcattct
      961 ttgctgagct gtataatgtg ccggaatcga cccggccttc aatgcatcca ggggaaggaa
     1021 ccaggtatgg ctcatttccg ggatcatccg gccattgtca tggactctct tgtccttgac
     1081 ggcccaaagt actttgctga gtaaatattg gttgtaatgg ctaaatggcc gattcccttc
     1141 cggtccactt tctttagttc taactacaag tgcagagtct attgcagtct ccgtataatc
     1201 cttgtaatca tcgtccggat cagctttcaa gtgtgttaat tccggcaata tggctggtaa
     1261 gccggtatta tgactgagta atgagcctat tgtaaatgta ttaagccggc tgccagaaaa
     1321 ttcgcggaat tggaatcttc ccagcgaatc atcaatattg atcagtccta gttgcatcat
     1381 gtgatatgta agtagtatga gttgagcatg acttacacct cccgcgacaa ataaggaagt
     1441 atccgtaaca ggcgattggc tttgatgttc taaggtgccc cactggtaag tataggtaga
     1501 atcaccatca atgataccta tgacggtgca ggaaccctga ttgaggtcga tatcgtcatc
     1561 aagacggact attttccgga tttcgttttc taagtgcaac cgttgtgctc ttcccggaag
     1621 agatgccata attgtcaagg tgagtagggg gactaaggtc ctgaaacaca tgggtcaaac
     1681 gatggatttg ccggccaatg attgccggat ggaataatcc atagcttttt ctgccatagg
     1741 tctggctatc ttgtccagct gatgtatgat gtcatgaatg gcatccttat cttcgccttt
     1801 gaccacttcc ttcagcttgt ccgcgataag tatcaaaggg gcgaattctt catcagtaaa
     1861 taattctgcg tgctgattca gaaatttttc tgtggatagg atgatgttgt tggcttcatt
     1921 tctggcttct atcaatgctt ttattttgac atcttccttt gcatttttga tgctatccag
     1981 aagcatcaat gccatttctt cttcacttat gccgtattgt gccttgattt ctacctgtgt
     2041 ttctgtgttg gagcgctctt cacgggcctt aaccaaaaga attccatcag catccacttt
     2101 aaagacgact tctatttttg gcaggccggc cggcatgggt ggaataccac gaagggtaaa
     2161 ttctccgagt ttgcggttgt cctttactaa atctctttcg ccctggaaca ccgaaatgcg
     2221 gagattgacc tggccatcga cactggtggt ataatgccgt cctgcacctg aaggtatttt
     2281 ggaattacgg ggtattatga catccatcaa tccaccaaca gtctcaatac caagggacag
     2341 cggcgtcaca tccagtagca acagatgact ttggtttcct gcaaggatat cagcctgaat
     2401 ggcagcaccc tgagcgacta cttcatcggg attgatcttg tcattgatgg gggcagaaaa
     2461 tatcccggac aggctctctt taacgaaagg tattctggta gatcctccga caagaatgac
     2521 ctctgcaatg tcttttgcat caaggcctgc atctttcagt gcggcttgtg tcagctccag
     2581 agattttttg atgaatggca ttgtaaggtt cttgtattct tctcgtgtaa gtataatttg
     2641 ttggttatca aaggaccctt caaatatgtc atggtcagca agatactttt tagccgtctc
     2701 acacaccaga cgcaattgct gcatttgacc cttgtccaga tcgacattat ttattccttt
     2761 ttttataaca aagtccaata ttgccttatc cacgtcatca ccacctaacc aggtatcgcc
     2821 tttggtagat agtacttcga acacgccacc ctcaagggtg agtatactca ggtcaaaggt
     2881 tccacctccc atatcataca ctgcaatatt tcttccttct tctgaagttt tgtcaaatcc
     2941 ataagcaaga gctgcggcag taggctcatt gataatccga agcacatccc agcctgccag
     3001 ttttccggcg tccttggtgg cctgtcgctg gctgtcattg aaatatgcgg gaaccgtaat
     3061 gacgcattcg ctgaccttgc ggccgagctc ctcttctgca attcttttca actccttgag
     3121 gatcaaggcg gataattcta ttggcgtata aaatttattg ccggatttaa tcttgaccaa
     3181 cgattccgta tcggtatcaa cgatttcgta actgaaaaaa tttctgtgtg cttcgacatc
     3241 tttaaaagat cgacccatca atctcttcac ggaaaagatt gtgcttgccg gatccgaaac
     3301 aagtttttcc tttgccaaac taccaacgat tgcattgtgg ttttcatcca ggtaaatgac
     3361 agagggaacc agtgaagttg aattcttgtg gtgtaatacc aat
//
LOCUS       DAOQMC010000246         3390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_14948_length_3390_cov_4.967016, whole genome shotgun sequence.
ACCESSION   DAOQMC010000246 DAOQMC010000000
VERSION     DAOQMC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14948_length_3390_cov_4.967016"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKV31_08880"
     CDS             <1..637
                     /locus_tag="PKV31_08880"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB93011.1"
                     /translation="FMKNVSLIFNVILTIAVAFLFYLNFKKPVQVTTITTSSGKDSTI
                     KVDIPETLPEGVPMGKILYVDIDTINEKYTFIKNQKAIIERQGAQAEASLKAKAQSLQ
                     SEFIEYQKKAQAGELTQQQAMAMEKSLTDKQQALAQQEQKLSTQLVEKTQKIQEELNA
                     KMRKELKVFMEKYGADYILGYTEGANILLPNPKLNITKEVLNRLNESDKKK"
     gene            644..2671
                     /gene="uvrB"
                     /locus_tag="PKV31_08885"
     CDS             644..2671
                     /gene="uvrB"
                     /locus_tag="PKV31_08885"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNB93012.1"
                     /translation="MSFHLESAYKPTGDQPEAIRQLVDGVNGGDRAQVLLGVTGSGKT
                     FTMANVIKELNRPTLILTHNKTLTAQLYGEFREFFPDNAVEYFVSYYDYYQPEAYIAV
                     TDTFIEKDLQINEEVDKLRLRATSSLLSGRNDVIIVASVSCIYGMGNPDDYKNSIIRL
                     QRGQIISRNAFLYKLVDSLYARTEAMLTRGHFRVKGDTVDIALPYVEHGLRIHFYGDE
                     IEEIEILEVESAKAIQKIETAAIYPANLYVAPKDKLNDILHSINHELKEHESYLESEG
                     KYLEAKRIHERTTFDIEMIRELGYCSGIENYSRFFDGREPGTRPFCLLDYFPDDFLMI
                     IDESHVTIPQLRGMWGGDRSRKTNLVNFGFRLPSAMDNRPLNFEEFESLIHQVIFVSA
                     TPGDYELEQTDGVFVEQLIRPTGLVDPPIEVRPSINQIDDLMEEVAVRIERNERVLVT
                     TLTKRMAEELAKYFQNLNIKTKYIHSEVDTLERVEILRDLRIGEFDVLVGVNLLREGL
                     DLPEVSLVAIIDADKEGFLRNTRSLTQTAGRAARNVNGKVIFYADKITDSMQKTIEET
                     NRRRAKQLAYNEKHNITPKTLAKTKEEILSRSSILDIRGKKQEAYYQEAEHPDIAADP
                     VVAMMSQDQLMALITETENKMKKAAKDLDFLSAAQFRDELFALKKKLKFAS"
     gene            2674..>3390
                     /locus_tag="PKV31_08890"
     CDS             2674..>3390
                     /locus_tag="PKV31_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93013.1"
                     /translation="MMVYYRKKLVILLFMATVVISSFGFKPPLKGRADLPNVLIIEIK
                     GMTFEMMERAYTPNIKFLIKNGFLGSMKYKNPEEPSTGISMNFRRKLGTMAMCMDSMK
                     VCQTSLNTVGAFNESFKGPFSKIRKNYRSNESMVKDFINNSNGTNVRFAMLSFRAFTQ
                     KQGFVDEGQIRELSTIDSLIGVVFKSYQEKVKWEQTTVFIVSDGSAAHEYRESELYKN
                     TPIVIKGPGIPIVYNVENVVS"
BASE COUNT         1037 a          669 c          794 g          890 t
ORIGIN      
        1 ttttatgaaa aatgtatcct tgattttcaa tgttatcctg acgattgccg ttgcttttct
       61 tttttatctg aattttaaga aaccggtaca ggtaacaact attacaacat cctccgggaa
      121 ggattctacc attaaggtgg atatccctga gacgttgccg gaaggtgtcc ctatgggtaa
      181 gattctttat gtcgatattg ataccatcaa tgaaaagtat acttttatca aaaatcaaaa
      241 agcaatcatc gaaaggcagg gcgcacaagc tgaggcaagt ctaaaagcca aggcgcagtc
      301 tctccagtcg gagtttatcg agtatcagaa aaaggcgcag gcaggtgaat tgacacaaca
      361 acaggcaatg gcaatggaaa agtcattgac tgacaaacaa caggcgttgg cccaacagga
      421 gcaaaagctt tctacccagc ttgtagaaaa aacgcaaaag attcaggaag agctgaatgc
      481 aaagatgagg aaggaactca aggtatttat ggaaaaatat ggcgcggact atatacttgg
      541 ttataccgaa ggcgctaata tattgcttcc taatccgaaa ctaaatatca cgaaagaggt
      601 tttaaataga ctgaacgaat ctgataaaaa gaaatagaat taaatgtctt ttcacctgga
      661 atctgcctat aagcctaccg gagatcagcc ggaagcgata cgccagcttg tggatggtgt
      721 aaatggcgga gatcgtgctc aggtgctgct gggtgtaacc ggctctggca agacattcac
      781 catggccaat gtcattaagg agctcaacag gcctactctg atactcaccc acaataagac
      841 attgacagcc cagttgtatg gggagttcag ggagtttttt ccggataatg ccgtggagta
      901 tttcgtctcc tattatgact attaccagcc cgaagcctat atcgctgtca cggacacctt
      961 tatagaaaag gacctccaga tcaatgagga ggtggataag ttgagactca gagctacttc
     1021 cagtctgtta tcaggccgaa atgacgtgat cattgtggca tccgtatctt gtatctatgg
     1081 catgggcaac ccggatgatt ataagaatag tatcatccgc cttcaacgtg ggcagatcat
     1141 ttcaagaaat gcctttctgt ataagctggt ggatagcctt tatgcccgaa ccgaagccat
     1201 gttgacccgc ggacatttcc gggttaaggg agatactgtc gatattgctt tgccatatgt
     1261 ggaacatggt ctgagaattc atttttatgg cgatgaaatt gaagagattg aaatcctgga
     1321 ggtggaatcc gcaaaggcga tacaaaagat tgaaacagct gccatttatc cggccaatct
     1381 ttatgtggcg cctaaggata aactcaatga catccttcat tccatcaatc acgaattgaa
     1441 ggaacacgaa tcctatctgg aatccgaagg aaaatacctc gaagccaagc gcatccacga
     1501 acgcaccacc ttcgacattg agatgatcag ggagttaggc tattgcagcg gtattgagaa
     1561 ttattccagg ttttttgatg gcagggaacc cggtacaaga ccgttttgcc tcctcgatta
     1621 ttttccggat gatttcctga tgatcataga tgaaagccat gttaccatcc cacagttacg
     1681 cggtatgtgg ggaggtgacc gcagtcggaa aaccaatctg gtaaattttg gattcaggtt
     1741 acccagtgcc atggataaca ggcctttaaa ttttgaagaa ttcgagtctt tgatccatca
     1801 ggtgatcttt gtatcagcaa cccccggtga ttacgaattg gagcagactg acggtgtctt
     1861 tgtggaacag ttgatcaggc ctacgggctt ggttgatcct ccgattgaag ttcgcccttc
     1921 catcaatcag attgatgatc tgatggaaga agtggctgtg agaatagaac gcaatgagag
     1981 ggttttggtt acaacgctca ccaaacgaat ggctgaggaa ttagctaaat actttcaaaa
     2041 tcttaatatc aagaccaagt acatccactc agaagtggat accctcgaaa gagtagaaat
     2101 attacgagac cttcgtatcg gagaatttga cgtactggtt ggggtaaact tgctgcgaga
     2161 gggtttggat ctgcctgagg tgtcattggt tgcgatcatt gatgctgata aggaaggctt
     2221 cctgagaaat acgcggtctc tgacacagac agccggaagg gcggccagaa atgtaaatgg
     2281 taaggttatt ttctatgctg acaagataac ggatagcatg cagaaaacaa ttgaagaaac
     2341 caaccggcga cgggcaaaac agttggcata taatgagaaa cacaatataa cccctaagac
     2401 tttggccaag accaaagagg aaatcctatc ccgaagttcg attctggata tccgcggcaa
     2461 aaaacaggaa gcatattacc aggaggctga acaccctgat attgctgcag atcccgttgt
     2521 agctatgatg tcacaggatc aattgatggc attgattact gagacggaaa ataaaatgaa
     2581 aaaagctgcc aaagaccttg atttcctttc cgctgcacag ttccgggatg aattatttgc
     2641 cttgaaaaag aaattaaaat tcgcaagcta gaaatgatgg tttattatag aaaaaagctt
     2701 gtgatattgc tcttcatggc aactgtggtt atctcctctt ttggatttaa accaccattg
     2761 aaaggacggg ccgacctgcc gaatgtgttg attattgaga ttaagggcat gacctttgaa
     2821 atgatggagc gtgcttatac tccaaatatc aaattcctca tcaagaatgg tttccttggt
     2881 tcgatgaagt acaagaatcc ggaggaacct tctaccggta tttccatgaa tttcaggcgt
     2941 aagttaggca ctatggcaat gtgtatggat tccatgaagg tttgtcaaac ttctctcaac
     3001 acagtcggtg ctttcaatga atctttcaaa ggtcctttca gtaaaatcag gaagaattac
     3061 cggtccaatg aatcgatggt gaaggatttt atcaataaca gcaatggtac taatgtcagg
     3121 tttgccatgc tttccttcag ggcatttacg caaaaacaag gatttgtgga cgaggggcag
     3181 atcagggaac tttcaaccat tgatagcctt ataggcgtag tttttaagag ctaccaagag
     3241 aaagtcaaat gggaacaaac cactgtcttc attgtctctg acggatctgc agcccatgag
     3301 tatcgtgaaa gtgagttata taaaaacact ccaatcgtaa tcaagggacc cggaattcca
     3361 attgtgtata atgtggagaa tgtggtatct
//
LOCUS       DAOQMC010000247         3378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15039_length_3378_cov_4.287993, whole genome shotgun sequence.
ACCESSION   DAOQMC010000247 DAOQMC010000000
VERSION     DAOQMC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15039_length_3378_cov_4.287993"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKV31_08895"
     CDS             complement(<1..285)
                     /locus_tag="PKV31_08895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB93014.1"
                     /translation="MLEFHSDIKTYFNIQYLTSRDHIIPYLSQFMDTCSSIRVLEVGC
                     GEAGVLKAFLERGHDCTGIELEQKRIESARELFRETEHGDRIKFIVKNIYD"
     gene            complement(286..744)
                     /locus_tag="PKV31_08900"
     CDS             complement(286..744)
                     /locus_tag="PKV31_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNB93015.1"
                     /translation="MPVVFDLMPFAKQLEVKYVQGKPHFFDKTRRVHLKVTPEEIVRQ
                     SLIIKLHADFGIPWVRMRTEYGIKHNSLQKRIDLLIYDKKGKPWMIIETKAPTVPLSQ
                     LVIDQVAFYNNVIFAPWLMVANGNEAMFASVDFQSKATAEVFEFPLNQWL"
     gene            complement(744..1955)
                     /locus_tag="PKV31_08905"
     CDS             complement(744..1955)
                     /locus_tag="PKV31_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNB93016.1"
                     /translation="MATFLCIASYYKGNDFLIACKEAGNKVYLVTSKGLEDKPWARSH
                     IDNIFYIQEDQNDEWNLDDLIKGTAFLMRSEKIDRVVALDDFDVEKAAALRETFRLPG
                     MGQTTAHHFRDKLSMRMKAFEKGIKVPAFSGLFNDDEVNHYLSTVKAPWLIKPRGEAS
                     ATGIVKCHTREDAWTVIQSLGDKRYQYLIEQFRPGDVYHVDAINDKKKVVFAQVSKYV
                     NTPMEVSHGGGIFRSHTVEYDSYDDKELQKLNADIMKAFGMNYSATHTEFIKCHEDGH
                     FYFLETASRVGGAHLAEMVEFATGVGLWTEWAKVESHSASGTEYRIPKKEKFHAGIII
                     SLSKYECPDYSDFDDDEIVWKLDMEYHIGIIVKSMSLQRIKELLEKYADIIKNEFHAA
                     GPLKPVKHKNS"
     gene            complement(2013..3278)
                     /locus_tag="PKV31_08910"
     CDS             complement(2013..3278)
                     /locus_tag="PKV31_08910"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNB93017.1"
                     /translation="MIKMISLILGGGAGTRLYPLTKHRSKPAVSIGGKYRLIDIPISN
                     CLNSGVKRMFVLTQYNSASLNKHIKNTFQFDKFSHGFVDILAAEQTPSSKQWFQGTAD
                     AVRKSMHHLVNHDYDYVLILSGDQLYQMDFEEMVMAHIEKNAELSIATIPVVARDATG
                     FGIMKVDDTSRITSFVEKPNAEDLPEWVSPMSESNQKAGKNYLASMGIYIFNRPTIEE
                     LFEQNPAADDFGKEIIPIAISEGRKVVSYQYEGYWTDIGNIRSFFEANLELTDDVPKF
                     NLFDNARMIYTRARMLPPSKIFGSKIQHSIISEGAILHAESIIRSVIGIRSRIGANTT
                     IKASIVMGNDFFETIEDIVSNKGRIPMGIGNNCYIENAIIDKNARIGSDVRIQGSTTK
                     ANVEEEGYCIVDGIVVVKKNAVIPSGTVI"
BASE COUNT          981 a          754 c          560 g         1083 t
ORIGIN      
        1 atcgtatata ttcttgacaa taaacttaat tctgtcacca tgctctgttt ctctaaatag
       61 ctcacgggcc gattctatcc ttttttgctc cagttcaatt cccgtacaat cgtgccctct
      121 ttcgagaaaa gctttcaata ctccggcttc accgcaacct acttccaata ctcgtatcga
      181 agaacaagta tccataaatt gagaaagata gggaatgatg tgatctcttg acgtcaggta
      241 ttgaatatta aaatatgtct taatatctga atgaaattca agcatttata accattgatt
      301 taaaggaaat tcaaatactt ctgctgttgc tttcgattga aaatcgactg aagcaaacat
      361 ggcttcatta ccattggcaa ccataagcca cggtgcaaaa ataacattat tataaaatgc
      421 tacctgatcg attacaagtt ggcttaacgg aacagtgggt gctttcgtct ctataatcat
      481 ccagggcttg cccttcttgt cataaatcaa caaatcaatc ctcttttgaa gtgaattatg
      541 ctttatccca tattcggtcc gcattctcac ccatggaata ccaaaatcag catggagttt
      601 gatgataaga ctctgtctta caatttcttc aggagtcact ttaagatgga ctctccttgt
      661 cttgtcgaag aaatgcggtt tgccttgcac atatttgacc tcaagttgtt tggcaaatgg
      721 catcaaatca aacacaacag gcatcagctg tttttatgct tgactggctt caaagggcct
      781 gctgcatgaa attcattctt tattatatca gcatattttt ccagcagttc cttgattctt
      841 tgaagactca ttgacttaac tataattccg atgtggtatt ccatatccag cttccagact
      901 atttcatcat catcaaaatc cgaataatca gggcattcgt acttagacag agaaataata
      961 atacctgcgt gaaacttttc ttttttaggt atcctatact ctgtcccgga tgctgaatgc
     1021 gactcaacct tggcccattc cgtccaaagg cctactccgg tagcaaactc gaccatttcc
     1081 gccagatggg caccgccaac tcgtgaggca gtttcaagaa aataaaaatg cccatcttca
     1141 tgacatttga taaactcggt atgtgttgca ctataattca taccaaaggc cttcataata
     1201 tctgcattga gcttttgaag ttctttgtca tcgtaactat catactctac ggtatgtgag
     1261 cggaatatcc ccccaccatg agatacttcc atcggagtat tgacatactt gctcacctga
     1321 gcaaatacaa ccttcttttt atcgttgatg gcatccacat ggtagacatc gccgggcctg
     1381 aattgttcaa tcagatattg atatcttttg tcccccagac tctgaataac cgtccatgcg
     1441 tcttccctgg tatggcattt tacgataccg gtcgcagaag cctcaccccg aggttttatc
     1501 aaccagggcg ctttaacagt tgaaagataa tgatttactt cgtcatcatt gaacaagcct
     1561 gaaaatgccg gcaccttgat gcctttttca aacgccttca tcctcatgga taatttatcg
     1621 cgaaagtgat gtgcagtagt ctgacccatt cccggcaatc tgaaagtttc gcgtagggca
     1681 gcagcttttt ccacatcaaa gtcatccaac gcaactaccc ggtcaatttt ttcagaacgc
     1741 atcagaaaag cagtcccttt gatcaaatca tccaaattcc actcatcgtt ttgatcttcc
     1801 tggatgtaaa aaatgttatc tatatgggac ctcgcccaag gcttatcttc taatcccttg
     1861 gaagtaacca aatatacttt attaccggct tccttacagg ctatcaaaaa atcatttccc
     1921 ttataatagc tcgcgataca caaaaaagta gccatattgc ttacattaat tttcttattt
     1981 ataatttatt caggctctga ttaatcaata ttttatatca ctgttccgga cggtatgaca
     2041 gcatttttct ttacaaccac tatgccatcg acgatacaat atccttcctc ttctacattt
     2101 gccttcgttg tgctcccctg aattcggaca tctgagccaa ttcttgcatt tttatcgatg
     2161 atggcatttt ctatataaca gttattaccg attcccattg ggatcctacc tttattagac
     2221 actatatcct caatcgtttc aaaaaaatca tttcccatga cgattgaagc ctttatagtt
     2281 gtattggctc caattcttga acgaataccg atgaccgaac ggataatgga ttctgcatgc
     2341 agaattgcac cttctgatat tatgctgtgt tgaatcttgc ttccgaaaat ttttgaggga
     2401 ggaagcatcc ttgccctggt ataaatcatt cttgcgttgt caaaaagatt gaattttgga
     2461 acatcatccg tcaattccaa attggcttca aagaaggatc gaatatttcc aatatcagtc
     2521 cagtagcctt catactgata actgactacc tttctacctt cagatattgc gatgggaatg
     2581 atttctttcc caaaatcatc tgcagcagga ttttgctcaa atagctcttc gattgtaggc
     2641 ctattgaaaa tataaatgcc catcgatgca agataatttt tacctgcttt ttgattggat
     2701 tcagacatgg gagataccca ttcaggtaaa tcttctgcat ttggcttttc tacaaaactg
     2761 gttattcggg aagtatcatc gactttcata atgccaaacc ctgtggcatc acgagctaca
     2821 acaggaatag tcgcaattga caattctgca ttcttctcta tatgagccat gaccatttcc
     2881 tcaaaatcca tttgatacaa ttgatcacct gacagaatta agacataatc ataatcgtga
     2941 ttgaccaggt ggtgcattga tttccggacc gcatcagccg taccttgaaa ccactgctta
     3001 ctacttggcg tttgttcggc agcaagaata tctacaaatc catgagagaa tttgtcaaac
     3061 tgaaatgtat tctttatgtg tttgttaagc gatgctgaat tatattgggt caacacaaac
     3121 attctcttaa cccctgaatt cagacaatta gaaataggta tatcaatcaa acggtattta
     3181 ccaccaatag atacagcagg cttggaacga tgcttagtta aaggataaag tcgtgtcccg
     3241 gctcccccgc ctaaaataag gctgatcatt ttaatcatag tcaatcattt tatatattca
     3301 ttcgacttgt aatattaata taatttttaa aaattatgtt aattccttat aaagattcaa
     3361 ataattatgt gcagcgct
//
LOCUS       DAOQMC010000248         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15053_length_3376_cov_5.566396, whole genome shotgun sequence.
ACCESSION   DAOQMC010000248 DAOQMC010000000
VERSION     DAOQMC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15053_length_3376_cov_5.566396"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PKV31_08915"
     CDS             <1..640
                     /locus_tag="PKV31_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB93018.1"
                     /translation="LRENNTAVLGQLKDLSVITNSQAESIKKSLENIGAKDNYIQTIQ
                     QAAARKDSLNLSLVMNLKGVLGNLNDQDVEVKVDKGIVMISLSDKMLFKSGSYDISEN
                     AGAVLAKVAQVLNNQPDLEILIEGHTDNVPISKPGIKDNWDLSALRATSIARALQTRF
                     GIDPKRITAGGKSEFSPLTTNDTAEGRSVNRRTRIIIMPQLDQFFKLLERKI"
     gene            754..1101
                     /locus_tag="PKV31_08920"
     CDS             754..1101
                     /locus_tag="PKV31_08920"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNB93019.1"
                     /translation="MLIQVFKSKIHRATVTEANLNYVGSITIDEDLMEAANLYEGERV
                     QVVNNNNGERLETYVIRGERGSGDICLNGAAARKVEVGDTVIIIAYGFMTPEEARSFK
                     PAAVFPDSNNKLV"
     gene            1104..2195
                     /locus_tag="PKV31_08925"
     CDS             1104..2195
                     /locus_tag="PKV31_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB93020.1"
                     /translation="MQRKKFIVDFLKFLLFLAIGAGILFWVYISQSRAFQEQCAIDGI
                     PEEQCSLIRKLIADFRSVNILWLVAVIVAFQLSNVVRALRWQMICKPLGYKIKLGNAF
                     WTIMLSYFANLGLSRAGEVIRAGSLARYEKIKLDNVVGTVALDRFLDLVTMSIAILIA
                     FFFQYEEIQSYLASNMRDFDFAAFSRGWIFQTFVTGIVLFFLLVILFRNRIRQSLIYL
                     KIKNFTKGVIAGMKTIRKIERPFLFAFYSILIWVFYFLMTYFAFFSFPPTASLGIQAA
                     LLVFVFGSFGILIPSPGGMGTYHALVVAALAIYGIRGDDAFSYANIVFFNIQILTTIL
                     FGVLSLIFLPINNKHYIPHHILISDGHEK"
     gene            2185..2652
                     /gene="rfaE2"
                     /locus_tag="PKV31_08930"
     CDS             2185..2652
                     /gene="rfaE2"
                     /locus_tag="PKV31_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNB93021.1"
                     /translation="MKNKVLNREEVILQARTWQQQGLEVIFTNGCFDILHPGHVRYLA
                     QSRTLGDRLIVALNDDDSVRRLKGESRPINTLADRAEVVAALCCVDAVTSFSEDTPYD
                     LISALIPDVITKGGDYMPDNVVGADIVEEAGGKVVIIDFQEGYSTTSILKKSK"
     gene            complement(2680..>3376)
                     /locus_tag="PKV31_08935"
     CDS             complement(2680..>3376)
                     /locus_tag="PKV31_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB93022.1"
                     /translation="TKIVTENDRVRRLYIDPRTEETFTAIPGQFITFDLPVGEKRLQR
                     WKSYSIANQITTGEIELCIAKQEGGLGSTYLCEEVKVGDVLKFKGPDGMFYLPDPLPP
                     TVVMICTGTGIAPFRAMLQAALNHDKYKNTQFHLIFGTRYETDILYKEDLEAFSKYPN
                     FKLDIALSRHIGDGNYHKGYVHSIYLNHYNSEKTDALFMLCGWQEMVDEATDILQNAM
                     LMEKTRIMVELYG"
BASE COUNT          892 a          693 c          816 g          975 t
ORIGIN      
        1 cctgagagag aacaatacag ctgtgctggg tcaattgaag gatttgtccg tcattaccaa
       61 cagtcaggct gagtccatca agaaatcatt ggaaaacatt ggtgcgaagg ataattatat
      121 ccagactatc caacaagctg ctgcccgtaa ggattcgctc aatctgtcac ttgtcatgaa
      181 ccttaaaggt gtcctgggta atctcaatga tcaggatgtc gaggttaagg tggacaaagg
      241 cattgttatg atttcacttt cggacaaaat gttgtttaag agtggaagct atgatatttc
      301 agaaaatgca ggagctgtat tggccaaggt ggctcaggtg ctcaacaatc agcccgatct
      361 ggaaatattg atagagggac ataccgacaa tgttccaatc agcaagccgg gaatcaagga
      421 taactgggac ttgagtgcct tgcgagccac atccatagca cgtgcccttc agacccggtt
      481 cggcatcgat ccgaagagga taacagcagg tggcaagagt gaattttccc cacttactac
      541 caacgacact gcagaaggaa ggtctgtcaa tagaaggaca agaatcatta tcatgccaca
      601 attggatcag ttcttcaagc tgttggaaag gaaaatataa tccggagaaa ctttgacaag
      661 caatacactg tttaataata tattcggtga caataggtat ctgttgtcac tgtttttttt
      721 attccaatta aaaaccgtaa atttgtgccc actatgctaa tccaggtatt caaatcaaaa
      781 attcaccgtg cgactgtcac agaggccaat ctgaattatg ttggtagcat caccattgat
      841 gaagatctca tggaagctgc taatctctat gagggtgagc gcgtccaggt agttaataat
      901 aacaacggtg agaggcttga aacttatgta atccgggggg agagaggctc gggagacatt
      961 tgtctgaatg gcgctgctgc acgcaaagtg gaggtaggtg atactgtgat tattatcgcc
     1021 tatggattta tgacacctga agaggcccgg tcattcaagc ctgccgctgt tttccctgat
     1081 agcaacaaca aactggtgtg acgttgcagc ggaaaaagtt cattgttgat tttctgaagt
     1141 ttttgttgtt cttggcgatc ggtgccggta ttttgttttg ggtgtatata tctcaaagca
     1201 gggcatttca ggagcaatgc gctattgatg gcattccgga agaacaatgc agtctgatcc
     1261 ggaaattgat tgctgacttt agatccgtca acattctgtg gttggtggca gtcattgtcg
     1321 cattccagct tagtaatgtg gtacgggctt tacgatggca aatgatatgt aagcccctgg
     1381 gctataaaat caaattagga aatgccttct ggacgattat gctcagttac tttgccaatc
     1441 taggtctttc cagagcagga gaggtgatca gagccggaag tcttgccaga tatgaaaaaa
     1501 tcaaactgga taatgtggtc ggtacggtgg cgctcgatcg tttccttgat ctggtgacta
     1561 tgagtattgc catattgatt gcattcttct tccagtatga agaaattcaa agctatctgg
     1621 catccaatat gagggatttc gacttcgctg cattcagtcg tggttggata tttcagacct
     1681 tcgtcacggg aatagtcctg ttttttctac tggttatact tttcagaaat cgaatcagac
     1741 aatctcttat ttatcttaag attaagaatt ttactaaagg ggtgattgca ggaatgaaga
     1801 cgatcagaaa gatcgaaaga ccatttctgt ttgcttttta cagcattctg atatgggtct
     1861 tctattttct tatgacctat ttcgcctttt tctcttttcc acctactgcg agcctgggga
     1921 ttcaggcggc gttgctggtc ttcgtattcg gtagtttcgg catcctgatc ccatcgcccg
     1981 gaggtatggg gacttatcat gccctggtgg tggcagcgct tgccatttat ggtatccgcg
     2041 gggatgatgc cttcagctat gcgaatattg tatttttcaa tattcagata ctcactacta
     2101 tcctgtttgg tgtactttcc ctgatatttc tgccaatcaa caacaagcat tatatcccac
     2161 atcacatatt gatttctgat ggtcatgaaa aataaggttc tcaatcggga agaagtgatc
     2221 ctgcaggcca ggacctggca acaacagggc ctggaggtta tatttaccaa tggctgcttc
     2281 gacatactgc atccgggtca tgtccgttat ttggcccaat ccagaacttt gggagatcgg
     2341 ttaatcgtgg cactcaatga tgatgattca gtgagaaggc tgaaagggga aagcaggccg
     2401 atcaatacac tggccgatag ggcagaagtc gtggctgcct tgtgttgtgt ggatgcagtg
     2461 acaagttttt cggaggatac tccgtatgat ctgatcagtg ccttgatccc tgatgtgata
     2521 accaaaggag gcgattacat gccggacaat gtggtgggtg ctgatatcgt agaggaagca
     2581 ggtggaaagg tcgttatcat cgattttcag gaggggtatt ccacaacgtc tattctgaaa
     2641 aaaagcaaat agatcctgtc ccctatggat caatgatcat cacccatata actccaccat
     2701 gattctggtc ttttccatca acatggcatt ctggaggatg tctgtagcct catcgaccat
     2761 ttcctgccag ccgcacagca taaacaatgc atcggtcttt tcagaattat aatgattgag
     2821 ataaatggag tggacatatc ctttgtggta atttccgtct ccgatatgtc tgcttaatgc
     2881 tatatccagc ttgaaattag gatatttgct gaatgcctcc agatcctcct tatacaggat
     2941 atcagtctca tatcgggtac cgaaaatcaa gtggaattgt gtgttcttgt atttatcatg
     3001 attgagtgca gcctggagca tggcgcgaaa cggggcaata ccggtgcccg tacaaatcat
     3061 gactacagtc ggtggtagtg gatcaggcag ataaaacata ccatccggac ctttgaattt
     3121 caatacatcc ccgaccttca cttcttcgca taagtaggtg gagcccaggc caccctcctg
     3181 cttggcgatg cagagctcta tttcgccggt agtgatctgg ttggcgattg aatagctttt
     3241 ccacctttga agccgttttt cgcctaccgg gaggtcgaat gtgatgaact ggcctgggat
     3301 ggcagtaaat gtttcttcgg tccggggatc aatgtacaac cgcctgacac ggtcattttc
     3361 ggtaactatc ttagtg
//
LOCUS       DAOQMC010000249         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15130_length_3368_cov_3.703894, whole genome shotgun sequence.
ACCESSION   DAOQMC010000249 DAOQMC010000000
VERSION     DAOQMC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15130_length_3368_cov_3.703894"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PKV31_08940"
     CDS             <1..1137
                     /locus_tag="PKV31_08940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNB93023.1"
                     /translation="NLEKKRLEFTTDLKKAVDFADIIFLALPTPEGEDGSADLSYVLN
                     VANQLGKMISSYKIIVDKSTVPVGTADLVHQAIAQNAAAELFDVVSNPEFLREGVAVE
                     DFMKPDRVVIGTSSPRAREIIHRLYEPFVKQGNPIYFMDERSAEMTKYAANSFLATKI
                     TFMNEIANLCERVGANVDMVRLGMGADSRIGKRFLFPGIGYGGSCFPKDVKALHKIAD
                     QNDFNFRILDAVIQVNINQRTILFDKLHNFYKGNLNGKTIAIWGLSFKPNTDDIREAP
                     ALYIIDKLLDAGAKIRVFDPEAMDNVRRIYGEKLFYAVDQYEVLSDCDALMIMTEWNE
                     FRSPQFDSMFHSIKDKVIFDGRNIFDPKEMQEKGFNYESIGRPV"
     gene            1193..2470
                     /locus_tag="PKV31_08945"
     CDS             1193..2470
                     /locus_tag="PKV31_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNB93024.1"
                     /translation="MNTKIAVIGLGYVGLPLAVELAKHYHVIGFDINKNRLEELRKGK
                     DHTLEVEESNLVSVLNQNDQLGLSFTDDAQKIKGFDFYIITVPTPVNKYNYPDFTPLI
                     KASETVGKVISQDAVIIYESTVYPGATEEICVPVLEQFSGLEYNKGFFIGYSPERINP
                     GDKERTLTKIHKIVSGSTPETAGKVQDLYNSIITAGTHKAASIKVAEAAKVIENVQRD
                     INIAIANELARIFNRLNIDTTQVLEAAGTKWNFIKVKPGLVGGHCIGVDPYYLIQKAQ
                     EAGYHPEIMLAARRLNEGMSDYVAHELIKRMIDKEIRIKGSNVLVLGVTFKENCPDLR
                     NTKVVDIVNTLKDYKVNVDIYDPRVNKAELMAEYKISCLDQLENGKKYDGILLAVAHD
                     EFVTLPLEPLKNNPSVVYDIKSILPIEMVDQRL"
     gene            2525..2968
                     /locus_tag="PKV31_08950"
     CDS             2525..2968
                     /locus_tag="PKV31_08950"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB93025.1"
                     /translation="MDRFFKDLLEYGHHFNQLLADKFLEYSGRTSEKPVRLFNHIVNA
                     HHIWNNRIDPQQPVVGVWDIHDIGELKKMDATNYDRTLKILEKIEPEKMIVYSNSKGE
                     TFTNLVKDIIFHIINHSTYHRGQIAMEFRQSGLEPLNTDYIFYKR"
     gene            3078..>3368
                     /locus_tag="PKV31_08955"
     CDS             3078..>3368
                     /locus_tag="PKV31_08955"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB93026.1"
                     /translation="MANKNILITGGAGFIGSHVVRRFIQQYPDYHIFNLDLLTYAGNL
                     NNIEDIKDAPNYHFIKGDIRDLAFIRSVFSDYHITDVIHLAAESHVDRSISDP"
BASE COUNT         1011 a          749 c          706 g          902 t
ORIGIN      
        1 aatttagaga aaaaacgtct tgagtttact actgacctga aaaaggcggt tgacttcgct
       61 gacatcatct tcctcgccct gcctacaccg gaaggagaag acggttcggc cgacctttcc
      121 tatgtcttga atgtggcaaa ccagctgggt aaaatgattt cttcctataa aatcatcgtt
      181 gataaaagta cggttcccgt tggtacagcc gacctggtac atcaggcaat tgctcagaat
      241 gcagccgcgg agcttttcga tgtggtttcc aatccggaat ttctacgcga aggtgttgcc
      301 gttgaagact ttatgaagcc tgacagagtc gtcatcggta cctcatcccc acgtgcacgc
      361 gagatcatcc accgactcta cgagcccttt gtcaaacagg gtaaccccat ttacttcatg
      421 gacgaacgct ctgccgagat gaccaaatac gccgccaatt cattcctggc aactaaaatc
      481 acctttatga atgaaatagc aaacctatgc gaacgtgtgg gcgccaatgt cgatatggtt
      541 cgcctgggta tgggtgcaga ctcgcggata ggtaagcggt ttcttttccc gggcatcggc
      601 tatggaggat cctgctttcc caaggacgta aaggcccttc ataagatagc agatcagaac
      661 gattttaatt tccggattct ggatgcagtg attcaggtaa atataaatca gcgaaccatc
      721 ctgttcgaca aattacacaa cttctataaa ggtaacctca acggtaaaac cattgccatc
      781 tggggacttt ctttcaaacc aaataccgac gatatccgcg aagctcccgc tctgtacatc
      841 attgataaat tgctggatgc aggtgccaaa atccgcgttt ttgacccgga agccatggac
      901 aatgttagga ggatctatgg agaaaaattg ttttatgctg tcgaccagta tgaagtactg
      961 agtgattgcg atgccctgat gattatgaca gaatggaatg aattccgctc cccgcaattt
     1021 gactccatgt ttcatagcat aaaggataag gtgatttttg acgggcgcaa tatttttgac
     1081 ccgaaagaaa tgcaggaaaa aggatttaat tatgaaagta tcggccgccc cgtgtaatga
     1141 cccgggctca ccccgaaata attagcattt tttaatttaa atactatata acttgaatac
     1201 gaaaatagcc gttatcggcc tcggttatgt cggcctgcct ctggctgtgg aactggccaa
     1261 acactatcat gtaattggtt ttgacatcaa taaaaaccga ttggaagaat tgagaaaggg
     1321 taaagaccat accctggaag tggaagaatc aaacctggtc tccgtattga accagaatga
     1381 tcaactcgga ttatcattta cagatgatgc acaaaaaatc aaaggtttcg atttttacat
     1441 tatcaccgta cctaccccgg ttaacaagta taactatccc gattttacac ctttgatcaa
     1501 ggcaagtgag acagtaggta aggtaatcag tcaggatgcc gtcatcattt acgaatccac
     1561 ggtctatccc ggagctactg aagagatttg tgtgcctgtt ctcgaacaat tttccggatt
     1621 ggaatacaat aaaggctttt ttattggcta ttctcctgaa agaatcaatc cgggtgacaa
     1681 ggaacgtacc ctgactaaaa tccacaaaat tgtttcgggt tctaccccgg aaactgccgg
     1741 aaaagtgcag gatttgtaca attccatcat cacagccggc acccataaag ccgcctcaat
     1801 taaagttgcc gaagctgcca aggtcatcga aaatgttcag cgcgacataa atattgctat
     1861 cgccaatgaa ttggcaagaa tcttcaacag actcaacatc gacacgactc aggtactcga
     1921 agcagccggt acaaagtgga actttattaa ggtcaaaccc gggctggtag gcggtcactg
     1981 catcggcgtc gatccatatt atctcatcca gaaagctcag gaagccggtt atcatccgga
     2041 aattatgctg gctgcccgcc gcctcaatga aggcatgtcg gactatgtcg cacacgaact
     2101 gataaaacga atgatcgata aggaaatccg tatcaagggc tccaatgttt tggttcttgg
     2161 tgtcaccttc aaggaaaatt gtcccgacct cagaaatacc aaggttgtcg acatcgtcaa
     2221 tacccttaag gattacaaag tcaatgttga catctatgat ccgagagtca acaaggctga
     2281 actgatggct gaatacaaaa tttcttgcct ggatcagctc gaaaatggca aaaaatacga
     2341 tggcatatta ctggctgtcg ctcacgatga atttgttacc ttgcctctgg aacccttaaa
     2401 aaacaaccct tctgtcgtgt atgatatcaa aagtatcctt ccgatagaga tggttgatca
     2461 gcggttataa ccgtataagg ttggtgtgat ttttcatttc cataatcgac aaaatttgta
     2521 aaaaatggac cgttttttca aagatttgct tgaatatggt caccatttca atcaactcct
     2581 ggcagataaa tttctggaat attcaggccg tacttctgag aagcccgtca gactttttaa
     2641 tcacatcgtc aatgcccacc atatctggaa taacagaatc gacccgcaac agcctgttgt
     2701 aggcgtctgg gacattcatg atatcggtga gttgaaaaaa atggatgcta ctaattacga
     2761 ccggaccttg aaaatattag aaaaaataga gcctgaaaaa atgatagtgt attccaactc
     2821 aaaaggggag acctttacca atttggtgaa agacattatc ttccacatca tcaaccactc
     2881 cacctaccat cgaggtcaga tagctatgga gttcaggcag tccggacttg agccgctgaa
     2941 tacagattat attttttata aaagataaat gaaaaatatt ggtataattc tttgtttaaa
     3001 ttaaaaaatg ctccgtccat tgtacaaaaa caggaatttt accgaattgc atttttgcaa
     3061 aaattagata cttgagtatg gcaaataaaa atatcctcat caccggaggc gccggtttca
     3121 ttggttctca tgtggtacgt cgctttattc agcaatatcc ggattatcac attttcaatc
     3181 tggacttgct gacctatgcc gggaatctga ataatattga agatataaag gatgctccca
     3241 attaccattt tatcaaagga gacatccgcg acctcgcctt tattcgttca gttttcagcg
     3301 attaccatat aaccgatgtc atccacctgg cagcggaaag ccatgtggac aggtcgatct
     3361 ccgacccc
//
LOCUS       DAOQMC010000250         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15142_length_3366_cov_3.997584, whole genome shotgun sequence.
ACCESSION   DAOQMC010000250 DAOQMC010000000
VERSION     DAOQMC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15142_length_3366_cov_3.997584"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PKV31_08960"
     CDS             <1..645
                     /locus_tag="PKV31_08960"
                     /inference="COORDINATES: protein motif:HMM:NF036547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB93027.1"
                     /translation="NDHSLSILENYSWPGNIRELRNITEQLSVLSEERIITPEILLRI
                     APQLANRNLPSIIKGQEDDHFTERDILYKLLFEMKGDMNDLKTLVFELITRNQLNVGD
                     LSNLRQLKHPTSQQDFSAFSGFNNNQANQPAHTILEPSVPAAHHTYLPDEPDEEVYQH
                     AEEVEDVLSLDAMEKDLIQKALKKYNGRRKEAAKELGLSERTLYRKIKQYDING"
     gene            728..1036
                     /locus_tag="PKV31_08965"
     CDS             728..1036
                     /locus_tag="PKV31_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNB93028.1"
                     /translation="MFGNFEEQQKEMEKKLKAITVHKASSENEINITMNASLEVENIS
                     IDLSKLDLTSSDQLEDLILVTMNEAIQQAQLAQAAESQKMLANMMPGGLGDLGKLFGQ
                     "
     gene            1063..2280
                     /locus_tag="PKV31_08970"
     CDS             1063..2280
                     /locus_tag="PKV31_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB93029.1"
                     /translation="MLRHIFLLLLTCSFFAPIVNAQITDNLYAFYNFDDCSTRDVQGS
                     NHGVPHVTPDCVCGLTDMAIRFDGSQYIELGDKTSGLIPTLTGDEFTLVLSLTTSRKY
                     GPMTVFSIVEDCDSYGYELIYNPIQSKFTLNIRSQTELTSINFDDDPGACWQQVAINR
                     LFNRMDIIVNGQLKYTQKWVTNPDFRSLGLPILGGGGCTGKTTTRFIGLIDEVKVYNR
                     ILSVDNAKIVTSKVINKLNDHFQVIFKGQSYGVEFITNCPVNVSWLPTQGVSDPASNI
                     VELSPETTTKYSVSSFYEGCTDVDTILIKVLDESNIQCNKLLLPNSFTPNDDGLNDTY
                     GISTTYAIDGDCLFEIFNKYGGKVFSTKDPNGRWDGKIGNDVAPPGLYLYKVRYTCAD
                     KQYTTSGSVNIIR"
     gene            2385..>3366
                     /locus_tag="PKV31_08975"
     CDS             2385..>3366
                     /locus_tag="PKV31_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93030.1"
                     /translation="MLRFNLVVFFILTFLFSSFKAYNQSVFVFQGILKEADNSNPVED
                     AMVMLHPLGIGTTTDKVGFFKIEYTGNFSGNIHIKKEGFIDVDYAIQPADVNAAVPVI
                     ITMYRDAAYNARVQSTGTIPVISIDDESANDGSQEISSLLSASRDPFLSIAGYNLGVF
                     RFRLRGYDTEQNSVYLNGIQMNDPENGALVFGEWGGLNDVLRNTDANYGLTPVSFTFG
                     GIGSKTMINLRASEQRKTIRASYAISNRSYRNRIMATYNTGKMANGWSLSASASYRWA
                     QEGYAQGTFYKGISYYLGIEKELNKNHSLGLTIIGAPTQRGKSNGSVQEVY"
BASE COUNT         1075 a          647 c          686 g          958 t
ORIGIN      
        1 aatgatcaca gccttagcat tcttgagaat tattcctggc cgggcaacat tcgtgaattg
       61 cgaaatatta cagaacaact ttcggttctt tccgaagaaa gaatcataac gcctgaaatt
      121 ctccttcgga ttgctcctca actagccaat cgcaatcttc cttcaattat taagggacag
      181 gaagatgatc actttacgga gagggatatt ctatacaaat tgctttttga gatgaaaggc
      241 gatatgaatg atttgaaaac attggtcttt gaattaatta caagaaatca actgaatgtg
      301 ggcgatcttt ccaacctcag gcaattaaaa catccaacca gccaacagga cttttcagca
      361 ttttcaggat tcaataacaa ccaggccaat caacctgcac acaccatttt agaaccaagt
      421 gttcctgcgg ctcaccatac gtacttacca gatgagcctg atgaagaggt gtaccaacat
      481 gcggaagaag tggaggatgt tttatctctt gacgctatgg aaaaggatct gatacagaag
      541 gctcttaaaa aatataatgg tagacggaaa gaagctgcca aggagttagg cctttcggaa
      601 cgaacactat atcgaaagat taaacaatat gatatcaacg gatgattgta ttgtgcgcca
      661 gaaggaaatc ttaatcttta ttcaatatta ttgacttatt ttcgtttaaa atataaattt
      721 atttatcgtg tttggcaatt ttgaggaaca acaaaaggaa atggagaaga agcttaaggc
      781 tattactgta cataaggctt cttcggaaaa cgaaatcaac atcactatga atgcaagcct
      841 ggaggtggaa aatatttcca tagatctaag caaacttgac ctaacttcat cagatcaact
      901 cgaggatctt attctggtaa caatgaatga agccattcaa caggctcaat tagcacaggc
      961 tgccgaaagt caaaaaatgt tggctaatat gatgccggga ggattgggtg atctgggaaa
     1021 actatttggt caataaatca cagtctttgt taaatcctga caatgttaag acatatattt
     1081 ctcttattac ttacgtgtag tttttttgct ccgatcgtca atgcacaaat aactgataat
     1141 ctttacgctt tttacaattt tgacgattgc tcaaccaggg atgtacaggg tagcaaccat
     1201 ggagtgccac atgtaactcc ggattgtgta tgtggcctga cagacatggc gattcgtttt
     1261 gatggttcac agtatattga gttaggggat aaaacaagtg gattgattcc aacattgacc
     1321 ggagacgaat ttacgctggt actctctttg acaacctcta ggaagtacgg tcctatgaca
     1381 gtgttttcca tagtagagga ctgtgatagt tatggatatg aattgattta taaccctatc
     1441 cagagtaagt tcacactcaa tatacgatct cagacagaat tgacttccat caattttgat
     1501 gatgatccgg gagcttgttg gcaacaggta gccatcaaca gacttttcaa cagaatggat
     1561 atcatcgtca acgggcagtt aaaatatacc caaaaatggg tcactaatcc cgatttccgc
     1621 tcactgggcc ttcccatctt gggtggtggc ggttgcaccg gtaagacaac tacaagattc
     1681 atcggtttga tcgatgaagt aaaggtatat aacagaatac taagcgttga caatgcaaaa
     1741 attgttactt ctaaggtaat caataaacta aatgaccatt ttcaggtaat tttcaaagga
     1801 caatcctatg gagtagaatt tatcacaaat tgtccggtta atgtctcatg gctcccaact
     1861 cagggcgtat cagatccggc ctccaatatc gttgaattat cacctgaaac aacaacaaaa
     1921 tactccgtat cttcttttta tgagggttgt actgacgtgg atactattct aataaaggta
     1981 cttgacgaat ctaatatcca atgtaacaag ctactattgc caaatagctt taccccaaac
     2041 gatgatgggc tgaatgacac ctatgggata tctacaactt atgctattga tggtgattgc
     2101 cttttcgaaa tattcaacaa gtatggaggc aaggtattct ccacgaaaga tcccaacggc
     2161 cggtgggatg gtaaaatagg caacgatgtg gctccacccg gcttatattt atacaaagta
     2221 cgctatacct gtgcagataa acaatataca acttcaggga gtgtaaatat tattagatga
     2281 tagattgtgg ataacattcg catcccaata tacaaggtta atatgataaa aaaaggtaga
     2341 aaaattacat aaactctttt acatttgtaa tatatataga gtttatgctg agatttaatt
     2401 tagttgtatt cttcatactc acctttttat tttcttcctt taaagcctat aatcaaagtg
     2461 tattcgtttt ccagggcatc ctgaaagaag cggataattc caatcctgta gaggacgcca
     2521 tggtaatgct acatcccctg ggcattggta caactaccga caaagttggc tttttcaaaa
     2581 ttgaatacac cggtaacttc tccggaaata tacatatcaa gaaagaaggt ttcatcgatg
     2641 tagattacgc tatccaacct gctgacgtca atgcggcagt tcccgtaatt ataaccatgt
     2701 atagagatgc cgcatacaat gcaagagttc aaagtaccgg gacgataccg gtaatatcca
     2761 ttgatgatga aagtgcaaac gatggttcac aggaaattag cagcctgttg tcagcctccc
     2821 gggatccttt tctttcaatt gcaggttata atttgggcgt attccggttt agacttcggg
     2881 gatatgacac agaacagaat tcagtttact tgaacggtat tcaaatgaac gatccggaaa
     2941 acggagctct cgtatttgga gaatggggtg gtttgaacga tgtgttgaga aatacagatg
     3001 ccaattatgg tcttactccg gtatccttca cttttggagg catcggttcc aaaaccatga
     3061 tcaaccttag agcttctgaa caacgtaaga caatcagggc ttcctacgcg atctctaacc
     3121 gcagttatcg caacaggatt atggctacat acaatacagg caagatggcc aatggatggt
     3181 ctctttctgc tagtgcatca tacaggtggg cccaggaagg atatgcccaa ggtacctttt
     3241 ataagggtat ctcttattat ctaggcattg agaaagagct caataaaaat cactcattag
     3301 gattgacgat cataggtgcg ccaacccaaa gaggtaaaag caacggatct gtacaagaag
     3361 tgtacg
//
LOCUS       DAOQMC010000251         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15190_length_3359_cov_4.518160, whole genome shotgun sequence.
ACCESSION   DAOQMC010000251 DAOQMC010000000
VERSION     DAOQMC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15190_length_3359_cov_4.518160"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKV31_08980"
     CDS             complement(<1..648)
                     /locus_tag="PKV31_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNB93031.1"
                     /translation="MRYKLSNFNFDLPKNSIAQFPSEQRELAKMMVVHKDSGKIEHKI
                     FKDILDYFDSGDVMIMNNTKVFPARLYGKKEKTGATIEVFLLRELNREMKLWDVLVDP
                     ARKIRVGNKLYFNDAKGNEVLVAEVVDNTTSRGRTIRFFYDGTPEDFMKILDQLGNTP
                     LPKYISRDANDLDKERYQTVYAKSIGAVAAPTAGLHFSKELLKRLEIKGVDFAELT"
     gene            844..2748
                     /locus_tag="PKV31_08985"
     CDS             844..2748
                     /locus_tag="PKV31_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB93032.1"
                     /translation="MKACISVFLIYVVTIFNVINAQELEYLLPEATISSAFNKPPVNA
                     SFLIRHKADSNYQYTLKSTSGMLREDGIYLKAYNEGGLNTAGSRGYGSSHTKVFWNGF
                     LLNSPLNGTIDLGLQALDPLATDILFNKSNSPVEGSISGAGGSIWISDRIPKGDNAVR
                     AALSAGSFSTYKSALSYTAHRGRFSNHLLLSLMTSANDYRYPSVTDFRRPLVRLNNAD
                     VLLYGINNASSLILPSSRVDLRFNYTRAARGIPPPITSTGPIAFQQDEGIKLGMAWST
                     ALNSRSDLSFNAAYMRDVLFYYPVRNADSKTEYYVNAAPLSARWNYNRWSHHLNIHLS
                     STFYHVNSTELTAPDEVQTYGTIELDRDKAGLLQYGFSVQQLMVKTRLIEPSYRLYVG
                     TRIVKGLNWNLSFASTKQVPTINDRYWPLSGNPDLKVETNRSLELGLNYSQEFGNLQF
                     VKGSLAIFNGMVDDWIGWIQVSGDFWKPFNVQKVQSRGLDLRGDYHWAVSSRVKLLAS
                     YNLSISNPIVIKKYSGSPAQEGKRLIYTPAFQHAACISLELDNHLMKVMPRYTSSRFT
                     TADNKVEYSLDPFVLLDFEYNYHFKIRKSKFTAVFKVNNVFNEYYEMIAYRPMAGRYF
                     EVGLGAGFGQ"
BASE COUNT          927 a          661 c          691 g         1080 t
ORIGIN      
        1 cgttagttca gcaaaatcga ctcccttaat ctcaagcctc ttgagaagtt ccttgctgaa
       61 gtgcagtcct gcagtcggtg cagcaacagc accgatagac ttggcataga cggtttgata
      121 ccgctcttta tccaaatcat tggcgtcacg tgaaatatat ttagggagtg gagtgttgcc
      181 aagctggtcc aggattttca tgaaatcttc aggagtacca tcatagaaaa atcgaatagt
      241 ccttcctctt gatgtggtat tgtccacaac ctcagcgacc aatacttcat ttcctttggc
      301 atcattgaaa tataacttgt tgccaactcg aatctttctc gccgggtcaa ccagtacatc
      361 ccacaatttc atttccctgt tcaactcgcg aagtaaaaat acttcaatcg tagcgccggt
      421 cttttccttt ttgccgtata gtcttgccgg gaaaaccttg gtattgttca taatcatgac
      481 atctccggaa tcgaagtagt ccagaatgtc cttgaatatc ttgtgctcaa ttttgcctga
      541 atccttatga acaaccatca ttttagccaa ttctcgctgt tcagagggga attgggcaat
      601 tgagttcttc ggtaggtcaa aattgaaatt cgatagttta tatctcatga aattatattt
      661 cttattaccg caatttgagc gcaaaaataa tttttttgga caattacatc attttatttt
      721 ttctcatctt gctgtttcct ttatatttaa tatcaatctc ttgaataagt ctattaatgc
      781 caataaggtt caacaagttt atactttttt ctttttaaca taataatttt acttttgcga
      841 cctatgaaag cctgtatttc agtatttctc atctatgtcg taaccatttt taatgttatc
      901 aatgctcagg aattggaata cctgctccct gaagcgacta tttcttctgc tttcaataaa
      961 ccaccggtca acgcctcctt tttaatcaga cataaggcgg atagcaacta tcaatacacc
     1021 ctgaagtcaa cttcggggat gctgcgggag gatggcattt atcttaaagc atataatgaa
     1081 ggtggactaa atactgccgg aagccgaggt tacgggagta gccatacgaa agttttttgg
     1141 aatggctttt tactgaatag cccgctgaat ggtacgattg accttggatt acaggcactt
     1201 gatcctttgg ctaccgatat tttattcaat aaatcaaaca gtccggtaga agggagcatt
     1261 tccggtgccg gaggaagtat atggatctcg gatcgaattc caaaaggtga taatgcggtt
     1321 cgtgcagccc tgtccgcagg ttctttttct acctacaaat ctgccttgtc ttatactgca
     1381 caccggggaa gatttagtaa tcatcttttg ctatcgttga tgacatcggc taatgactac
     1441 cgttatcctt cagtgacgga ttttagacgt ccattagtca ggctgaacaa tgccgatgtc
     1501 ttgctttatg gaatcaataa tgcgtccagc ttgatcttac cttcttcaag ggtggatctc
     1561 aggtttaact atacacgggc agccaggggg attcctccgc cgattacaag tacagggcca
     1621 attgctttcc agcaggatga gggcattaag ttgggtatgg cgtggagtac agctttgaac
     1681 agtcgatcag atcttagttt caatgctgcc tatatgaggg atgttctgtt ttattacccg
     1741 gttaggaatg cggattctaa aacagaatat tatgtaaacg ctgcaccact gtcagccagg
     1801 tggaattaca atcgatggag tcatcatctg aatattcacc tgagcagcac tttttatcat
     1861 gtcaacagca ctgaactaac tgctcccgat gaagtgcaaa cctatggaac cattgaattg
     1921 gacagagaca aggccggtct gcttcaatac gggttcagcg tgcagcaatt gatggtaaag
     1981 acacgactga tcgaaccgtc ttaccggttg tatgtgggca caagaatcgt gaagggattg
     2041 aattggaatt tgtcatttgc ctctaccaaa caggtgccta ccatcaatga cagatactgg
     2101 cccctttcgg gcaatcctga cctgaaagtt gaaaccaatc gatccttaga gctgggtttg
     2161 aattattcac aagaatttgg caatctgcag tttgtaaaag gctccctggc aatttttaat
     2221 ggcatggttg atgattggat tggctggata caggtgagtg gtgacttctg gaagcctttc
     2281 aatgtccaga aggtgcagtc cagggggttg gaccttcgag gtgattatca ttgggctgtc
     2341 tcttcccggg tgaagctttt ggcgtcctat aacctgagta tttccaatcc aattgttatt
     2401 aagaaatatt ccggcagtcc tgctcaggaa gggaagcggc tgatctatac acctgccttt
     2461 caacatgcgg cctgcatttc cctggagttg gacaatcacc tgatgaaagt gatgccgcga
     2521 tatacgtcgt caagatttac aactgcagat aataaggtgg aatacagtct ggatcccttt
     2581 gttttgttgg attttgaata caattatcac tttaagatta gaaagtcaaa atttacggca
     2641 gtattcaaag ttaataatgt ttttaatgaa tattacgaga tgatcgccta tcgtcccatg
     2701 gcagggagat attttgaagt tggtctgggt gcaggttttg ggcagtaact aatttgaagg
     2761 cattttatta ggatagaaga atgtatttat tcaatgtttt tttatccgtt tttgtacttc
     2821 cttacatttc aggaagagga tgaaaaaaat tggataattg cagatgcctt gatagatcca
     2881 tgatgccagt ttggccggaa aaatattgaa atgactataa taatcagata atcatattaa
     2941 tatacattaa aaatattaat atgaatatga ttagtttatt gattattatt tactcatggc
     3001 tgcattccca cttttcatta tccccatttc agtccctcat tgtgccaatt atgttatcgt
     3061 accaattgaa gagatcaaaa agcgacaatc tatatacttt ttgtagatgt aagttgtgct
     3121 accgaatact acactggatg agctttgcag ccatggtatg cccggttaat cgctattgtt
     3181 ggaatgaacc ttttcaaaaa ggtcagtaac cagactgaag actgtccatc ctatgttgct
     3241 ctttatttgt cctgtaattg cttattaatc aaatatcatt acattttttt atttcaatat
     3301 gatattataa gcgtaacttt gtgctttact tttaaaagga gaaccttctt acatgagcg
//
LOCUS       DAOQMC010000252         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15450_length_3324_cov_5.237381, whole genome shotgun sequence.
ACCESSION   DAOQMC010000252 DAOQMC010000000
VERSION     DAOQMC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15450_length_3324_cov_5.237381"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            829..2115
                     /locus_tag="PKV31_08990"
     CDS             829..2115
                     /locus_tag="PKV31_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNB93033.1"
                     /translation="MELNYVSAAEALSLIKSNSRVFLHGSACTPVYLLEELAKNAGNY
                     KNVELVFISLQGPEMEIAKPEYADSFHVNSLFVSAPVRGAVNSGFADFVPVFLSDIPD
                     LFNDGIFPIDVAIIHVTPPDDHGYCSMGVSVDIARAAVNNAKKIIALVNPRMPRTMGD
                     GMIHYKHIDKMVFHPSELPEVDYSSKVTEIDRMIGRNIAEMVEDGSTLQLGIGTIPDS
                     VLTYLGNHKDLGVHTEMLSDGIIDLFLNNVINNRKKRIHPNRMVTSFALGTRRLYDFV
                     DNNAGLAFLDIDYVNNPSVIAKNDKVVAINSCIEIDLTGQVCSDSIGTYQYSGIGGQM
                     DFIRGAALSKGGKPIIAINSRTRKGQPRIVPTLKPGAGVVTSRGHVRYVVTEYGVVNL
                     FGLNMRQRAKALIEIAHPDDREMLHQSAYERFKIFL"
     gene            complement(2770..3117)
                     /locus_tag="PKV31_08995"
     CDS             complement(2770..3117)
                     /locus_tag="PKV31_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNB93034.1"
                     /translation="MIQIKRIYAKNEATDGYRVLVDRVWPRGVSKESAHIDEWLKELG
                     PSTELRKWFGHIPQKFPEFEQKYRYELASVTKTLQRLKSISKKQTLTLLYSAKDEIHN
                     QAMVLSKVLEELD"
BASE COUNT          912 a          650 c          672 g         1090 t
ORIGIN      
        1 atttaagctt ataagccacc ctttaagtaa atctttattc atgccggaga gggtcatgcc
       61 ttagcaggat tatatcgact ctcctccgat acctgacagg aagatttatt tattcgccgg
      121 aaatcaggat agagccatta gagttgccaa ttatttattc catttttagc acagacaaag
      181 caaaaggaat caacccgtat aataggctta ttgtcaccct catcaaaagc ccatttcatc
      241 ctataaccgg atacatggac tgcgtccatg ttatactccg gaaaatgggg tgcagttact
      301 gggttagtta ccatccaccc aaagtccctg gacatagatt tttttttaaa tatgcgcatt
      361 ttgcgttatc ttgtatcata tttccactgt tatgaagtcg ttccatatga tttttattgg
      421 cctgtactgc ctggccgcac tttattttac ctatttgtca tttacaacaa tgtttgttta
      481 tttttccaat aaatcaatgg gattccagga gtcttttctg gaaccgggca agaatcttat
      541 tttagcactt ttattcatct tttttactgc tgcttcatgg tttttgctga agagtcccaa
      601 ttatcataaa ttagggatga ttgtatttta tattccaatt gtaataattg gtttatatat
      661 cttgtgggct gtcatcatgc tgataggatc aggaggtaaa tggaattgat ttggcatatt
      721 gggcctcttt tttttgtact aaaggcatat tgttgaagtt attgatgata tagcataaaa
      781 tttcatttct ccgaaaattg ctcttatttt tggcgccaaa attttgaaat ggagttgaat
      841 tatgtttctg cagctgaagc cttatccctg attaaaagca atagccgtgt atttctgcat
      901 ggcagcgcat gtactcctgt ttatctattg gaagagttgg ccaaaaatgc cggtaactat
      961 aagaatgtag aactggtttt catttccctt caaggtcctg agatggagat tgccaagccg
     1021 gaatatgcag attcattcca tgtcaacagt ctttttgtca gtgctccggt acgtggggct
     1081 gtcaatagcg gttttgctga ctttgttcct gtcttcctga gtgatattcc ggatttgttt
     1141 aatgatggaa tcttcccgat tgatgttgcc atcatacatg tgacaccacc ggatgatcat
     1201 ggatattgct ccatgggagt atctgtcgat atagcacgtg ctgcggtcaa taatgcaaaa
     1261 aaaatcattg ctctggtcaa tccgcgaatg cccaggacaa tgggtgacgg gatgatccat
     1321 tataagcata tcgataagat ggtctttcat ccttctgaac tacctgaggt agattatagt
     1381 tccaaggtga cagaaatcga ccgtatgata ggcaggaata tcgctgagat ggtggaggat
     1441 ggatccacac ttcagttggg aattggtact atcccggact ccgttctgac ctaccttggc
     1501 aaccataaag atcttggtgt acatacagag atgcttagtg acggtatcat cgatctcttc
     1561 ctcaataacg tcatcaataa ccgcaagaag cgaattcacc caaatcgtat ggtgacaagc
     1621 tttgccctag gaacacgacg cctctatgac tttgtggata ataacgccgg attagctttt
     1681 ctcgatatcg attatgtcaa taatccttct gtcattgcaa aaaatgacaa ggtggttgcc
     1741 atcaactcat gcattgagat agatcttacc ggccaggttt gctccgatag catcggcact
     1801 taccaatata gtgggatcgg aggacaaatg gacttcatcc gcggcgcagc tctcagtaaa
     1861 ggcggtaaac caatcattgc catcaacagc cgaaccagga aaggacagcc acgtatagtc
     1921 ccgacactca aacccggtgc aggtgtggta acttcccgcg ggcatgtaag atatgtggta
     1981 actgagtacg gagtcgtcaa ccttttcggc ctcaatatgc gccaacgtgc gaaagcattg
     2041 atagaaatcg cacacccgga tgatcgggaa atgttgcatc agagcgctta tgaacgtttc
     2101 aagatttttc tttaagtaag gaatctactc ttaagtgcca atttttccct gttctgccgg
     2161 cctattacga ttaattttcg tcaaattgat tttacaaccg atgtcaagaa gttgatttct
     2221 attttttcat cttctatttg acggtattta gcactaccgg gagcatgata tttaatggat
     2281 atagttgcca ggcctttgtt taaatttatt ctcatctgtc gattttggaa atcaatggat
     2341 tgattatccg gccaccggga agatcgattg ggctgactac ccgatcgaat tactgtggtt
     2401 ataaggaaaa atgtcctcaa aacaataagg gaaacattaa tggctataaa gtatactgga
     2461 tgatattgac tggcctgatc atttgttggc gacaataaac cggttcgatt agccttttgg
     2521 gtatcgaaat tttaaagata ggtggggact aaactatcat ggatttaacc ctgcattgta
     2581 aataaaggat tattctattt aatcttttat tgtaattaat acagtatgaa tgaattgaag
     2641 atgagtagat tagttaattg ttcctgaaat attctgcaca acaatataaa atgtgttgcc
     2701 ttcccggtta ttgaatgtcg taaaccagaa atcagataac tcattacttg ggatgaatat
     2761 tttcgtattt caatctaatt cctccaatac tttactcaat accatcgcct gattgtgaat
     2821 ctcatcctta gccgaataga gtagggtgag ggtctgtttt ttcgagatgg actttagtct
     2881 ttgtaaggtt ttagttactg atgcaagttc atatcgatac ttttgctcaa attccggaaa
     2941 tttttgcggg atgtgaccaa accattttct caattcagtc gaaggcccca attctttcaa
     3001 ccattcatcg atatgggcgc tttctttgct tacacctctc ggccagacac gatcgaccag
     3061 tactcgatat ccatcagttg cttcgttttt cgcatatata cgtttgattt gaatcattgc
     3121 tacctccttt taatacaatc atgttgcatt taaaaattaa aacttgtaat gaggcaaatg
     3181 gttcattgga ccgggagcat aaattatcct ttctccggat tttattactg tgtaatgaaa
     3241 tccagagtta tgcccatttt ctcagttggt ttaacagtgc cttcagatct ttagggaatg
     3301 gtgcttcgaa aatcatttct tttt
//
LOCUS       DAOQMC010000253         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15522_length_3315_cov_3.998160, whole genome shotgun sequence.
ACCESSION   DAOQMC010000253 DAOQMC010000000
VERSION     DAOQMC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15522_length_3315_cov_3.998160"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKV31_09000"
     CDS             <1..686
                     /locus_tag="PKV31_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNB93035.1"
                     /translation="KEAKSSTSPSKSDKSSFVTGKKGQALDLAGGYLLYPEIASLNSL
                     KSFTVSLWANGLKNNGSHASILFSLGRANEWAGSINIMAETGWRKAGDDTLTIKGLIV
                     SNAANGASWQDSRNDIAKGGDQVFTKGNQWSQYVISYDATTEKFKVFANGVKVSNPDW
                     ETRTGLGELKVFTPSIPIIGAWWTNTGGTPDSWQVPFTGQIDEIRIFNKALSDAELSA
                     LYKLESAGR"
     gene            1383..2633
                     /gene="fabF"
                     /locus_tag="PKV31_09005"
     CDS             1383..2633
                     /gene="fabF"
                     /locus_tag="PKV31_09005"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNB93036.1"
                     /translation="MKRVVITGIGAITPIGNNIEQYNQGLKNGVSGAGPITRFDAANF
                     KTRFACEVKDFNPEDFIDKKEVRRMDLFTQFAMVCSDEALADSKLLEYTELDYDRIGV
                     IWGSGIGGLRSLEAEIETFARGNGIPRFNPFMIPKMISDIAPGYISIKHGFRGINYGT
                     VSACASATHAMMNAFDYIRLGRADVIITGGSEAAITATGMGGFSATKALSERNDDFLT
                     ASRPFDLERDGFVLGEGGGCLIFEDYEFAKKRGAKIYAEVVGSAATADAYHITAPHPE
                     GLGAKNVMKMALADAGISAESIDYINVHGTSTPLGDIAETTAIKDVFGDHAYRLNISS
                     TKSMTGHLLGGAGAIESIASVLSIRDQYVPPTINHFTDDPQIDPKLNLTFNQAQYREI
                     NYAMSNTFGFGGHNACIIIKKFED"
     gene            2715..>3315
                     /gene="rnc"
                     /locus_tag="PKV31_09010"
     CDS             2715..>3315
                     /gene="rnc"
                     /locus_tag="PKV31_09010"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNB93037.1"
                     /translation="MLGYTPAHLELYVQAFKHKSNSVEVNGHRQHNERLEFLGDAILN
                     SIVAEYLYKKYPNGDEGFLTKMRSKIVKRKTLDAIGARMGIDDLMTIMNKTQISRSML
                     GNALEALVGAVYLENGYAFTRKFIVLEILRKYLDIHRLEGMDDNFKSQLLEWCQKNNM
                     EVNYELVSKYKFEKRDRFKIAVMIQGEQVAEADDFNKKNA"
BASE COUNT          973 a          636 c          758 g          948 t
ORIGIN      
        1 gtaaggaagc caaatcttcc accagtcctt caaaatcaga taagtcatca tttgttactg
       61 gtaagaaagg tcaggcactg gatttggcag gtggatatct cctgtaccct gaaattgcct
      121 ccctaaacag cctcaaaagc ttcacagttt ccctttgggc aaatggcctg aagaataatg
      181 gaagccatgc aagcatcttg ttcagccttg gcagagccaa tgagtgggca ggatccatca
      241 atattatggc ggagacaggt tggcgtaagg caggtgacga cacattgacg atcaaaggcc
      301 tcatagtttc taatgctgcg aatggggcaa gttggcagga tagcagaaat gatatagcaa
      361 aaggtggcga tcaggtgttt accaaaggta atcagtggtc acagtatgtt atctcctatg
      421 atgcaacaac tgagaaattc aaagtttttg ccaatggagt gaaggtaagc aatcctgatt
      481 gggaaacaag gaccggttta ggtgaattga aagtcttcac gccatctatt ccaatcatcg
      541 gagcttggtg gaccaacact ggcggcacac cggattcttg gcaggtaccg tttaccggcc
      601 aaatcgatga aatccgtatt ttcaataagg cgctttcaga tgccgagttg agtgcactgt
      661 acaaactgga atctgcagga agataaaatc ttatcattca gaaaattaag gcacctttta
      721 cgggtgcctt tttttatttt cgaaactttt tggacattcc acaatacctt gagggtttta
      781 tcggttgatg atggatccca tgacaattcc tacgaataaa tggatggtgt ggttttggat
      841 ttttttcatt tgtttaattt atttcactac aatggtattt gtgtgttgta atttccaacc
      901 ggactttcat ttatacaata cagcaacaca ttatttagaa gaatattcaa ggtattttgt
      961 tgaatcggcc aaattgaata aatttcatat ggtctttgat gttgagacaa gctctccgga
     1021 gctaaaggca gtataatcct atgatgcctc tccgccgtta tttagttttt tagctccggt
     1081 attggagagt gcaccctcaa tctcttacta tttggtctga ttttgccact gaacaatcaa
     1141 aggattgttg tggaggggcg acttgattga aataagatat ttttattcga ttgactctta
     1201 ttataatttg attcttttac ctcatttgcc gtccgattat agattctatt tcaaaatgtt
     1261 atagacacaa ggcatacagt aatagctact ttgcaaatat gttattatat ttgcctcgca
     1321 gaagttcgct tttcgaaatg aatagtcatg aggctctctg caccagacaa agctataaac
     1381 ttatgaagag agtcgtaatt acaggcatag gtgccattac tcccatcgga aataacatcg
     1441 agcaatacaa tcaaggtctg aagaatggtg taagcggtgc cggccctatt acccggtttg
     1501 acgctgccaa tttcaagacc cgttttgcct gtgaagtgaa ggattttaat ccggaggatt
     1561 tcattgacaa gaaggaggtt cgtagaatgg atttatttac tcaatttgca atggtatgtt
     1621 ctgatgaagc attggctgac agtaagttat tggaatatac agagcttgac tacgacagga
     1681 ttggcgtaat ttggggatcc ggaataggag gattgcgttc tttggaagcg gaaatcgaga
     1741 catttgcacg aggaaatggt atccctcgtt ttaatccatt tatgataccc aagatgatct
     1801 ccgatattgc acccgggtat atcagcatca agcacggttt cagaggcatt aactacggga
     1861 cggtatctgc ctgcgcttcg gccacgcatg ccatgatgaa cgcctttgat tatatccgac
     1921 tgggcagggc cgatgtcatc attaccggag gttctgaagc ggcaataact gcaacaggta
     1981 tgggcggttt tagtgcaacg aaggctctga gtgaacgtaa cgatgacttc cttaccgcat
     2041 ccagaccttt cgacctggag cgggatggct ttgtattggg cgaaggtgga ggttgtctga
     2101 tttttgaaga ttatgaattt gccaagaaaa gaggggctaa aatttacgct gaagtagtag
     2161 gttctgcggc tactgcggat gcctatcata tcacagcgcc tcatccggaa ggtcttggag
     2221 ctaagaatgt tatgaagatg gcattagctg atgccggaat tagcgcagaa tccatcgatt
     2281 atatcaacgt tcacgggaca tcgactcctt taggggatat cgcagagaca acggctatta
     2341 aagatgtttt cggtgatcat gcatatcgat tgaacatttc atccactaaa tccatgacag
     2401 ggcatttatt gggtggagcc ggtgcaatag aaagtatcgc aagtgtactc tctattcgcg
     2461 atcaatatgt accacctacc atcaaccatt ttaccgatga tccacagatt gatccaaaac
     2521 taaatctgac ctttaatcaa gcacaataca gagaaataaa ttatgcgatg agtaatactt
     2581 tcggctttgg tggtcacaat gcctgtatta ttatcaaaaa atttgaggat taacattaaa
     2641 ctgtgaaagg cttattaaaa ttttttaacc gttatttttc gaaagacaaa cacttttgtg
     2701 gaaagttatc ttcaatgctg ggctacactc ccgcccacct ggaactatat gttcaagcat
     2761 tcaagcataa atcaaattcg gttgaagtca atggccatag acaacataac gaaaggcttg
     2821 aattcctggg cgacgctatc ctgaacagca ttgtagctga atatctttat aagaagtatc
     2881 ccaatggaga cgagggtttt cttaccaaaa tgcgctcgaa aatcgtgaaa cgaaagaccc
     2941 tggatgccat tggtgccaga atggggatcg atgacctcat gaccatcatg aataagactc
     3001 agatcagcag atctatgttg ggtaatgccc ttgaagccct tgtcggggcc gtttatctgg
     3061 aaaatgggta tgccttcacc agaaagttta tcgtcctcga gattttacgc aaatacctgg
     3121 atattcatcg tctggagggg atggatgaca acttcaagag tcaactcctc gaatggtgtc
     3181 agaaaaacaa catggaagtt aactatgagc tggtatccaa atataagttt gaaaaaagag
     3241 accggttcaa gattgctgta atgattcaag gagaacaggt agcggaggca gatgatttca
     3301 ataagaaaaa tgccg
//
LOCUS       DAOQMC010000254         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15575_length_3308_cov_4.579773, whole genome shotgun sequence.
ACCESSION   DAOQMC010000254 DAOQMC010000000
VERSION     DAOQMC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15575_length_3308_cov_4.579773"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKV31_09015"
     CDS             <1..622
                     /locus_tag="PKV31_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNB93038.1"
                     /translation="IEVVAQYEGEYGIELEPECACRLWIEIEANDAEALERELYALGD
                     VLSNVPSLNILIADEEATRQRIWKVRRVIGHAVRQYSVYREVDTVVPVSNMVRLIHTI
                     KEIGNRMKFRTLCYGHAGNGNIHVNVLRESMNDRQWAGILTDGVEALLKEVVQLDGVL
                     SAEHGIGLLNRPFMHLQFDAAEIALMRRIKAAFDPNGILNPRKVIP"
     gene            complement(603..1382)
                     /locus_tag="PKV31_09020"
     CDS             complement(603..1382)
                     /locus_tag="PKV31_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNB93039.1"
                     /translation="MDILRLKSYLDSLVLRYNHISFIQDDPISIPKQFSKRQDIEIIG
                     FWTAMLAWGRRSTIIAKAGELAAWMDNSPYDFILNHKETDRKPFLNFKHRTFQGDDAL
                     YFLHFFQSYYQHHNTLEDAFLVDGHNAGIKQGLIRFYETFTGDPLMMSRTKKHVSTPA
                     KNSACKRLNMFLRWMVRKDAAGVDFGIWTRLRMEDLMIPLDVHVAKVARRLGLLDRTK
                     DDWMAVDELTTRLIAFNPSDPIIYDYALFSLGVNDFRELPS"
     gene            complement(1398..>3308)
                     /gene="infB"
                     /locus_tag="PKV31_09025"
     CDS             complement(1398..>3308)
                     /gene="infB"
                     /locus_tag="PKV31_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNB93040.1"
                     /translation="KEIDEKIKSTMARLNVGGKNKRAKIRRDKRETIREKNEAMELAS
                     IDDKIQVTEFISVSELASLLNVPAIQVITTCMNLGVMVSINQRLDAEIIELVASEFGK
                     EIEFISAEDDTDFDTQEEDDPEEMLVERAPIVTVMGHVDHGKTSLLDYIRNANVVSGE
                     AGGITQHIGAYEVMVNGKKIVFLDTPGHEAFTAMRARGAKITDVAVIIIAADDNVMPQ
                     TREAISHAQAAGVPIVFAINKIDKPAADPERIKNELAQMNLLVEDWGGKYQSQEISSK
                     TGQNIDLLLEKILLESEVLDLKANPDRMANGTVIEASLDKGRGYVTKLLVQNGTLNQS
                     DYIVAGEFSGRIKAMFNERGKKLKSAGPSTPVLVLGLSGAPQAGEKFKEFENEQDARQ
                     IAARRAQINREQTNRATKRISLDDIGRRLALGTFKELNLIIKGDVDGSIEALTDSLIK
                     LSVETIQVNVIHKAVGQITESDVLLASASDAVIIGFQVRPSLGARKAAEKEGIQIKHY
                     SIIYEAINDVKAAMEGMLEPTKEETAVGEIEVREVYKISKVGTVIGGYVTEGKISKST
                     YVRIVRDGIVIYPTKENVHGEIGTLKRFKDDVKEVRAGLECGLTIRNFNDVRIGDTIE
                     GYDITEVKQKLS"
BASE COUNT          792 a          753 c          748 g         1015 t
ORIGIN      
        1 tatcgaggta gtcgcacaat atgaaggtga atatggaatt gagttggagc ctgaatgtgc
       61 ctgccgcctc tggatagaaa tcgaagcaaa tgatgcagaa gccttggagc gtgaattgta
      121 cgcgttgggc gatgtgttgt caaatgtccc ttccctgaat attttgatcg ccgatgagga
      181 agctacccgc caacggatct ggaaagttcg ccgggtcatt ggccatgccg tccgtcaata
      241 ttcagtgtat agggaggtgg atactgtagt tccggtaagc aacatggttc ggttgataca
      301 taccatcaaa gagattggca atagaatgaa attcaggacg ttgtgttacg ggcatgccgg
      361 aaatggcaat attcatgtca atgtattgcg ggagagtatg aatgaccggc agtgggccgg
      421 gattctgact gatggggtgg aggcattgtt gaaggaagtg gtccaactgg atggtgtcct
      481 ttcagcggag catggcattg gtttactcaa tcggccattc atgcatcttc aatttgacgc
      541 tgctgaaatt gccttgatgc gcaggataaa agcagcgttt gatcctaatg gaatattaaa
      601 tcctaggaag gtaattccct gaaatcattc acccctaaag agaataacgc gtaatcataa
      661 ataatggggt cggaagggtt gaatgctatg agcctggttg tcagttcgtc cacggccatc
      721 caatcgtcct ttgtgcgatc caataatccc agccgtcttg ccactttggc tacatgaaca
      781 tccagtggta tcatcaggtc ttccattcgt aagcgagtcc aaattccgaa gtctacaccg
      841 gctgcatcct ttctgaccat ccagcgtaaa aacatattca atcttttaca ggcggagttt
      901 ttggcggggg tggaaacatg ttttttagtt cgggacatca tgaggggatc gcctgtaaat
      961 gtttcataaa accggattag tccctgtttg atcccggcat tgtgtccgtc taccaggaat
     1021 gcatcctcca atgtattgtg gtgttggtaa taagattgga aaaaatggag aaagtaaagc
     1081 gcatcatcgc cttgaaatgt cctgtgctta aaattcagaa agggcttgcg atccgtttcc
     1141 ttgtgattca atataaagtc ataaggactg ttgtccatcc atgctgccag ttcgcctgcc
     1201 ttggcaatta tagtggatcg gcgtccccag gcgagcatgg cagtccagaa cccaatgatc
     1261 tcaatatcct gtctttttga aaattgtttg ggaatagaaa tgggatcatc ctgaatgaaa
     1321 gaaatatgat tatacctcaa cacgagactg tcgagataag atttcaatct gaggatatcc
     1381 atagccggat atatttatca ggagagtttt tgctttactt ccgttatatc gtaaccttca
     1441 atggtatccc caattcgtac atcgttgaaa ttcctaatgg tcaagccgca ttcaagtcct
     1501 gccctcactt ctttgacatc atctttaaac cgtttcaatg ttccgatttc gccatggaca
     1561 ttttctttcg tcggatagat aactatacca tcgcggacta tgcgtacata agttgatttg
     1621 ctgattttgc cttcggtgac atagcctccg atgactgtcc ccactttact gattttgtac
     1681 acttcacgga cttcgatttc accgacggcc gtctcttcct tggtcggttc aagcatgcct
     1741 tccatcgctg ccttgacatc attgattgct tcgtaaatga tggaatagtg tttaatctgg
     1801 atgccttctt tttcagccgc ctttcgtgca ccaagggatg gccttacctg gaagccgatg
     1861 attactgcat cggatgctga agcaagaagg acgtcggatt cagtgatctg tcccactgct
     1921 ttgtggatta cgttgacctg gatagtttca accgacagtt tgatcaggga gtcagtaagt
     1981 gcttcaatgg agccgtcaac atctcctttg atgatgagat tgagttcttt gaaggtacca
     2041 agagccaatc tgcgtcctat atcgtcaaga ctgatacgtt tggttgccct atttgtttgt
     2101 tctctgttga tctgtgccct tcttgctgca atttgtcttg catcctgttc attctcgaat
     2161 tccttaaatt tctcacctgc ctgtggagcc cctgatagtc cgagtacaag aaccggagtg
     2221 gaaggtccgg cactttttaa ttttttaccg cgttcgttga acatagcctt tatacgaccg
     2281 ctgaattcac ctgcgacgat ataatccgat tggttgagcg ttccattttg taccagtaat
     2341 ttggttacat agccgcgacc tttgtccagg gaggcttcga taaccgttcc gttggccatt
     2401 ctatccggat tggctttcaa gtccagtact tcagattcca ggagaatttt ttccagcaac
     2461 aggtcaatat tttgtcctgt tttggaagat atctcctgcg attggtattt accaccccaa
     2521 tcttcaacca ggaggttcat ttgagccaat tcatttttga ttctttccgg atcagctgcc
     2581 ggtttgtcaa ttttgttgat tgcaaaaaca atgggcacac ctgcagcctg ggcatgtgat
     2641 atggcttcac gtgtctgagg catcacattg tcatcggctg ctattatgat gacagcgaca
     2701 tcggtgatct tggcacccct tgcacgcatc gctgtaaaag cttcgtggcc cggagtatca
     2761 aggaaaacta ttttctttcc atttaccatc acttcatagg cgccgatatg ctgtgtgata
     2821 cctccggctt caccgctgac gacatttgca ttccgtatat agtccagcaa agaggtttta
     2881 ccgtgatcca catggcccat cacagtgacg atcggagctc tctcaacaag catttcttcc
     2941 ggatcatctt cttcctgagt atcaaaatca gtgtcatctt cggcgctgat gaattctatt
     3001 tccttgccga actcactcgc aaccagttct attatctctg cgtcaaggcg ctggttgatt
     3061 gataccatca caccaagatt catacatgtg gtaataacct gtatcgcagg aacattgagc
     3121 agtgatgcca actctgacac ggagatgaat tcggtaacct ggattttgtc atctatgctg
     3181 gcaagttcca ttgcttcatt tttctcccgg atggtttctc tcttatccct gcgtatttta
     3241 gcacgtttat tttttcctcc gacattgagg cgtgccattg tactcttaat cttttcgtca
     3301 atctcttt
//
LOCUS       DAOQMC010000255         3280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15787_length_3280_cov_4.483101, whole genome shotgun sequence.
ACCESSION   DAOQMC010000255 DAOQMC010000000
VERSION     DAOQMC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15787_length_3280_cov_4.483101"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>221
                     /locus_tag="PKV31_09030"
     CDS             25..>221
                     /locus_tag="PKV31_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93041.1"
                     /translation="MNAAKFNALQVENHKTSLLREIDLFTQQVSGLVEQEKQILNIQS
                     SGVVEMATLETQIQECENGIV"
     assembly_gap    222..231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <232..1182
                     /locus_tag="PKV31_09035"
     CDS             <232..1182
                     /locus_tag="PKV31_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB93042.1"
                     /translation="VAEKIKASQSELTEAERVYFTEKKQVNEDEQAIKLMQRSLAGIH
                     TIANEWKDKWHAAKFEMQRIEDNFSIEFGSTLPDEATTDESPDDTVLKELENQNQKIR
                     HSIHQFGEVNPLAVEAYNEIKARYDEIVSQKNDILDSKDKLLQALNELENTSTTKYLA
                     AYEQVRENFKMVFRSLFTEDDDCDLVIENPGTPLDSDIGIIARPKGKRPQSLSQLSGG
                     EKTLTAIAFLFSLYLLKPAPFCIFDELDAPLDDVNVEKLNKIIRKFSKESQFIIITHN
                     KATMAEVDVMYGVYMEQQGISGLSMVDFRTFEHNLVLEKV"
     gene            1350..1619
                     /locus_tag="PKV31_09040"
     CDS             1350..1619
                     /locus_tag="PKV31_09040"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNB93043.1"
                     /translation="MQNSMEQLIDKLKSEIIEALNLQDMSPDDIDTDAPLFGTGLGLD
                     SIDSLELMVLLERNYGIKIEDPREGRKVLESVRSMAQYIESFSNT"
     gene            1621..2835
                     /locus_tag="PKV31_09045"
     CDS             1621..2835
                     /locus_tag="PKV31_09045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNB93044.1"
                     /translation="MGQNNAEVWITGIGILSALGNGFEENWAALKSGKSALRPIELSG
                     SIHDGKFFAGMVSLSDEELVKQLSELNIRYPHNRPTLMSLYTARQALRNADIEMFTDK
                     IVLISVTTVGGISSTEKHFDNLSLNKTKATQEFLSEMDCGSISRNVCSYLGLSKPHYM
                     ISTACSSSNNAIMLGARLIKAGKAEVVLAGGSDSLSKFVLNGFHSLKNLDPKPCTPFD
                     KDRFGLNLGEGAAYLVLESSKHATNRGKSSLAVVAGYGNISETYHITGSSPHGMGAYG
                     AMSLALTSANLNPQEIDFIHSHGTSTIDNDLAESNAIKRLFGNDIAFGSSKGYTGHTL
                     AAAGAISSVLSIGMMQHGFIFPTINFRHPIPETGLSPVTTIKENQHLRHIMVNSFGFG
                     GNNTSLIFSKAA"
     gene            2832..>3280
                     /locus_tag="PKV31_09050"
     CDS             2832..>3280
                     /locus_tag="PKV31_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93045.1"
                     /translation="MMFISSISYTASSFSEDKGFFKISGPDYSGHFEKNEIRRLSPII
                     ITAVGCGLETNTNTNRELCGIITGTGRGCLSKTEQFLRDITTYKETALNPTHFIQSTN
                     NTIGGMIALKLNVNTYNMTYVNQGLTVLNVLTDTEILVKENPGKNVL"
BASE COUNT         1088 a          647 c          692 g          843 t
ORIGIN      
        1 gaattgagta gtacaggaag tttgttgaac gcagctaagt ttaatgccct tcaagttgaa
       61 aatcataaaa cttcattgct tcgggaaatt gatttattca cccagcaagt ttccgggttg
      121 gtcgaacagg aaaaacaaat cctcaatatc caaagttcag gtgttgttga aatggcaaca
      181 ttggaaactc aaattcaaga atgcgaaaat ggcatagttg annnnnnnnn ngtggctgaa
      241 aaaataaaag cgtctcaatc ggaactgact gaggctgaga gggtgtattt tactgagaag
      301 aagcaggtaa acgaggacga acaggctatc aaattgatgc agaggtcact cgcaggcata
      361 cacacaatcg ccaatgaatg gaaagataaa tggcatgcag cgaagttcga aatgcaacgg
      421 atcgaagata atttctccat tgagttcggt tcaaccttgc ccgacgaagc aactacagat
      481 gaaagtcctg atgatacagt attaaaggaa ctggaaaacc aaaatcaaaa aatccgacat
      541 tccatccatc agttcggaga agtaaatcct cttgccgtag aagcatacaa tgagatcaag
      601 gccagatatg atgaaatcgt aagtcaaaaa aatgacatat tagattcaaa agacaaatta
      661 ctacaggccc tcaatgaact ggaaaatact tcaactacga agtatcttgc cgcctatgaa
      721 caagtaagag aaaattttaa aatggtgttc aggagtcttt tcacagaaga tgatgattgc
      781 gatctggtca tagaaaatcc cggaacaccc ttggattcag atatcggcat catagccagg
      841 ccaaagggca aaaggccgca aagtctcagc cagttgtcag ggggagaaaa aacgctcact
      901 gccatagctt ttcttttctc attgtatttg ctaaaaccgg ctcctttttg tattttcgac
      961 gaattggatg ctccacttga tgatgtcaat gtggaaaaat taaacaagat aatcagaaaa
     1021 ttcagcaagg aatcgcaatt tatcatcatc acacataaca aggctaccat ggcagaagtt
     1081 gatgtcatgt atggtgttta catggaacaa caaggcatca gtgggttaag tatggtggat
     1141 ttcaggactt tcgaacataa tcttgtgttg gaaaaggttt agtgaaaatg taaacatttt
     1201 tagaaaaaaa ttaatatgtt tgtatctaat aatggaacag acagagtgct tattctccgg
     1261 gaattcacgc gattggatta gcatagtgaa ttatcaagac aatcagcgat cgaacagcaa
     1321 aaataaaata ccaattatta aaaaattctc tgcaaaatag tatggaacaa ttgatcgaca
     1381 aattgaaaag cgaaattatt gaagcattga acctgcagga tatgagtcca gatgatatcg
     1441 acacggatgc cccattgttt ggaacagggc taggtctcga cagtattgat tctttggagt
     1501 taatggtttt gctggaaaga aattacggaa taaaaataga agatcccaga gaaggccgga
     1561 aagtactgga gagtgttcga tccatggccc aatatattga gtcgtttagt aatacttaaa
     1621 atgggacaaa ataacgctga agtctggatt accggcattg gcatcttatc agcccttgga
     1681 aatggctttg aagaaaactg ggctgcactc aaatcaggaa aaagcgcact cagaccgatc
     1741 gaattgtcag gctcaattca cgatggcaaa ttttttgccg gaatggtttc tttgagtgat
     1801 gaagaacttg tcaaacaact ctccgaacta aatatccgtt atccccacaa caggcctaca
     1861 ttgatgtcgc tttatactgc ccgacaggca ttaaggaatg ctgatattga aatgtttacc
     1921 gataagattg tactcatcag cgtaactaca gtaggaggca tttcttcaac cgaaaaacat
     1981 ttcgacaacc tgagtctgaa taaaaccaag gcaacacagg agtttctcag tgaaatggat
     2041 tgcggcagta ttagtcgtaa tgtgtgtagt tatcttggcc tgagcaagcc acattatatg
     2101 atcagcactg cttgttcttc ctctaataac gccatcatgc ttggtgccag gttgattaaa
     2161 gccggtaaag ccgaagtagt actggccgga ggaagcgatt cattgagtaa gttcgtactc
     2221 aatggtttcc attcattaaa aaatctggat cccaaaccct gcactccatt tgataaagat
     2281 cgatttggac tgaatctggg tgaaggagcg gcatacctgg tactggaaag cagcaaacat
     2341 gcgacaaaca ggggaaagtc ttccctggcc gttgttgccg gctatggtaa tatcagtgaa
     2401 acttatcaca tcaccggctc gtcaccacat gggatgggag cttatggcgc gatgagttta
     2461 gccctcacct cagccaacct aaatcctcaa gaaattgact ttatccattc tcacgggaca
     2521 agtacaattg ataacgattt ggcagagtcc aacgccataa agagactttt tggcaatgac
     2581 attgcattcg gatcaagcaa agggtataca ggccacacac ttgccgcagc cggagccatt
     2641 tcgtcagtcc tttccattgg tatgatgcaa catggattca tatttccaac tataaatttc
     2701 cggcacccaa ttcctgaaac aggactttcc cctgtaacca ctatcaaaga aaatcaacat
     2761 ttacgccaca taatggtaaa ttcatttggt tttgggggca ataatacgtc cctgatattc
     2821 tcaaaagcgg catgatgttt atttccagca tatcctatac agcatcatct ttctcggaag
     2881 ataaaggctt ttttaagatc tcagggccgg attattcagg acattttgaa aaaaacgaaa
     2941 tacggaggct cagtcctatc ataataactg ctgtcggttg tggccttgag acaaacacca
     3001 atactaacag agaattatgc ggaatcatca caggaacagg tagaggctgc ctatccaaaa
     3061 cagaacaatt tctaagggat atcacgacct ataaagaaac agcgctgaat cctacccact
     3121 ttattcaatc caccaataat accattgggg gtatgatcgc tcttaaattg aatgtcaata
     3181 catacaatat gacctatgtc aaccaagggc tgactgtgct aaatgtcctg actgacaccg
     3241 aaatactggt caaggaaaac ccgggcaaaa atgtcttgtt
//
LOCUS       DAOQMC010000256         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_15811_length_3277_cov_3.489448, whole genome shotgun sequence.
ACCESSION   DAOQMC010000256 DAOQMC010000000
VERSION     DAOQMC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15811_length_3277_cov_3.489448"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /gene="ligA"
                     /locus_tag="PKV31_09055"
     CDS             <1..1983
                     /gene="ligA"
                     /locus_tag="PKV31_09055"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNB93046.1"
                     /translation="LGDTEYDFLFKSLESAEAAHPEWKVKNSPTDRVGLDITENAQQI
                     AHLKPMLSLANSYNEQDLIEFDQQIKRMLKLDSTQDIEYSVEPKYDGGGISLVYTENQ
                     LSRGVTRGDGVSGEEITNNVKSIKTIPLYVEFNKLGIHTIEVRGEAIISKANFEKVNQ
                     QRLAQGQPLFANARNAATGGLRTKDPKDTESRKMDAFIYTISYAVDFEGNDILNSFTT
                     QFGGIDAIGSLGIKTPNQGRMLCRNITEVIEFCHLWQNQREEFPYEIDGMVIKLNNTK
                     WQEELGTTAHHPRWAIAFKFQAKQTATRLIDIEYQVGKIGSITPVGKLEPVALAGVTI
                     SSVSLHNEEFIQSRDLRLGDMVVVERAGDVIPYIVKSLPDVRTGTEKAIVFPSSCPSC
                     HSNLIKPQEEAVWRCVNPDCPEQLKQRLIFFASKDAMDIEGLGKSIVEKFFDLGWLNS
                     FADIYRLSADKISGLEGFGRKSADNLINAIEKSKSNPIHRLLHALSIHHVGRKVAKLL
                     AEEIDSILDLAGKTQEQMETIRDIGPIVAQNVIKYFEDPRNIDMLKELEGLGVNMLAT
                     NQDHKSLIQVTGHYLSGKTILFTGTLHQMGRKEAQEKAEALGAKNLSAVSANLNILVV
                     GENAGSKLKKAKDLGSVEIWTEQEFLDRLAELQTNS"
     gene            2038..3063
                     /gene="pheS"
                     /locus_tag="PKV31_09060"
     CDS             2038..3063
                     /gene="pheS"
                     /locus_tag="PKV31_09060"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNB93047.1"
                     /translation="MSDTHYSKISEMAEDIEAFPVSNKEDLEAFRIAFLGSKNKLKDL
                     FALMGSVAAEHKKAFGQALNGLKKSAEEKFNSLSASLNNQGESLTSGKMDLTRPGYPT
                     TLGSRHPVSLTLNRIIEIFTKIGFTVEENREIEDEWHNFTALNTPEDHPARDMTDTYY
                     LANDFTRMLRSQTSTVQVHVMESQKPPIRIISPGRVYRNETISARSHCQFHQVEGLYI
                     DKNVSLADMKQTLNYYARAMYGPKARTRLRASYFPFTEPSAEMDVYWGLETEEDRRIT
                     KGPGWLEILVCGMVDPAVLENCGIDPDVYSGFAWGMGIERQAMLKYKITDIRHFFEND
                     IRFLSQF"
BASE COUNT         1000 a          705 c          738 g          834 t
ORIGIN      
        1 cttggtgaca cggaatatga ttttcttttc aagtcattgg aatcagcaga agcggctcat
       61 cctgaatgga aggtaaagaa ctcccccact gaccgagttg ggctggacat tacagaaaat
      121 gcacaacaaa tagctcattt aaagccgatg ctgtctctgg ccaattccta caatgaacag
      181 gatctgattg aatttgatca acagatcaaa cggatgctga agcttgatag tacacaggat
      241 atcgaataca gtgttgaacc caagtatgat ggtggaggca tatcacttgt ctatacagag
      301 aatcaactga gccggggagt cacacgtggt gacggagttt ccggtgaaga aatcaccaat
      361 aatgtcaaga gcatcaaaac cattccgctc tatgttgagt tcaataaact cggtatccat
      421 accatcgaag tacgcggtga agctatcatt tccaaagcaa attttgaaaa ggtcaatcaa
      481 cagcgccttg ctcaagggca acccttgttt gccaatgcca gaaatgcagc taccggaggc
      541 ctaaggacca aggacccgaa agatacagaa agcaggaaga tggatgcctt catctatacc
      601 atttcatacg ccgttgattt tgaagggaat gatatattga atagcttcac tacacaattt
      661 ggaggtatcg atgccattgg cagtttaggc attaaaaccc ctaatcaagg caggatgctg
      721 tgtaggaata tcactgaagt aatcgaattc tgccaccttt ggcaaaacca gcgcgaggag
      781 ttcccgtacg aaatagatgg tatggtcatc aagctcaaca atacaaaatg gcaagaggag
      841 cttggcacaa cagcacatca tcctcgatgg gccattgcat tcaagttcca ggccaaacag
      901 actgctaccc gacttatcga tatagaatat caagtcggaa agattggctc gattaccccg
      961 gtaggaaagc tggagcccgt cgctttggca ggggtaacca tcagttccgt ttccctccac
     1021 aacgaagaat ttatccaaag ccgcgatctt aggctgggcg atatggtcgt cgtcgagcgt
     1081 gccggtgatg tcattccata catcgtcaaa tcattgcctg atgtcagaac agggacagaa
     1141 aaggcaattg tttttccctc cagttgccca tcatgccatt ccaacctgat aaaaccgcag
     1201 gaagaagcgg tatggcggtg tgtcaatccc gattgccctg aacagcttaa acagcggttg
     1261 attttcttcg cctccaagga cgctatggac atagaaggtt taggtaagtc catcgtggaa
     1321 aaattttttg atttgggctg gttgaattca tttgcagata tataccgatt atcagcggat
     1381 aagatatctg gcctggaagg attcggtcga aaaagcgctg ataaccttat caacgccatt
     1441 gaaaaaagta aatccaaccc gatccacaga ttgctccacg ctctttctat ccatcatgtc
     1501 ggccgaaaag tagcaaagtt gcttgctgaa gagattgaca gcatcttgga ccttgcaggc
     1561 aagacacagg aacagatgga aaccatccgc gatattggcc ctattgttgc acaaaacgtt
     1621 atcaaatatt tcgaggatcc acgcaatatc gacatgctga aagaattaga gggtttggga
     1681 gtaaacatgt tagcgactaa tcaggaccac aaatcactta ttcaggtcac aggacattat
     1741 ctgtcaggga aaactatctt gtttacagga actctccatc aaatggggcg taaagaggct
     1801 caggagaaag ccgaggccct tggggccaag aatctgagtg ccgtaagtgc caatctgaac
     1861 attcttgttg taggcgaaaa tgcaggtagc aagctcaaaa aggcaaaaga tctgggcagt
     1921 gtggagattt ggacagaaca agagtttctg gacagactgg ccgaattaca gacaaattcc
     1981 tgaaatttca ataataacaa agcaattcct tatttttgcg acataataaa gatagcaatg
     2041 agcgatacac attactctaa aataagtgag atggcagagg acatcgaggc atttccggta
     2101 agtaataagg aagatctcga agcatttagg atagcttttc ttggttccaa aaacaagctg
     2161 aaagacctat ttgccctaat gggcagtgtg gcggctgaac ataaaaaagc tttcggtcag
     2221 gcactcaacg ggttgaaaaa aagtgccgag gaaaaattca acagtctttc tgcctcatta
     2281 aacaatcaag gagaaagcct tacttccgga aaaatggacc tcaccagacc gggctatcca
     2341 actaccttag gatcccggca tcctgtttca ttgaccctta atcgcatcat cgaaatattt
     2401 accaaaattg gatttacggt tgaagagaat cgcgaaattg aagatgagtg gcacaatttt
     2461 acagcattga acactccgga agatcatccc gcgcgtgaca tgacggatac ctattacctt
     2521 gccaatgact tcacacgcat gctcagaagt cagacatcga cggttcaggt gcatgttatg
     2581 gaaagtcaaa aacctcctat ccgcattatt tctccgggac gcgtttatcg taacgaaaca
     2641 atctctgccc gctcccactg ccagtttcat caagtagaag gcttatatat cgataagaac
     2701 gtaagtctcg ccgatatgaa gcagacattg aactattatg ccagggccat gtatggtccc
     2761 aaggccagga ccaggcttcg tgcctcatat tttccattta ccgagccaag tgcagagatg
     2821 gatgtttact ggggactgga aacagaagaa gacaggcgga ttactaaagg ccccggctgg
     2881 ctggagatac tggtttgtgg aatggtcgat cccgccgtat tggaaaattg cggtatcgat
     2941 ccggatgtgt atagtggttt tgcctggggc atgggtatag aaagacaagc aatgcttaaa
     3001 tataaaatta ccgatatcag gcatttcttc gagaatgata tccgtttttt gagccagttt
     3061 taagaaacca tgcttgaata agtaaatcta ttgtcaagaa aataattctt cgtttttcta
     3121 gcaatattgt atttcttagc acttttttct ccggcttatt aagccacaga cgattccagc
     3181 gctaatccag tctcttaatt tcgagtttaa aatcagttct acctgatcta aaaccatcat
     3241 atcatcgttt tttgtttcta ttgatgccgg gtttttt
//
LOCUS       DAOQMC010000257         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_16574_length_3187_cov_3.790549, whole genome shotgun sequence.
ACCESSION   DAOQMC010000257 DAOQMC010000000
VERSION     DAOQMC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16574_length_3187_cov_3.790549"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKV31_09065"
     CDS             <1..702
                     /locus_tag="PKV31_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNB93048.1"
                     /translation="KLDNKYYVTESERIFLEEGAIVECCNINTTTGPVYLGKNSMIME
                     GANIRGGLALCEESIVKMGAKLYGASTFGPHCKVGGEVNNSIFQGFSNKGHEGYLGNS
                     VIGEWCNFGADTNASNLKNTYKKVKIYNYRQNISIDSGLLFLGLIMGDHSKSGINTMF
                     NTGTVVGVFANIFGTGFPPGHIPSFSWGGAEHMEPYQFEKAIEVAEAVMSRKNQHLTP
                     EYKAILRHIYDNTHH"
     gene            717..1409
                     /locus_tag="PKV31_09070"
     CDS             717..1409
                     /locus_tag="PKV31_09070"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNB93049.1"
                     /translation="MKLPVNTWTKLLSYLVKVPVSRSESINDGALYVDLVKGRLQLST
                     DHAVYSFDDKYDNFVKALKAVKADLFRNKSILVLGGGLGSIPYILEKNHQFQGELTMV
                     ENDPAVIRLFLEYSLPRMNTLIDIRESDAIIFMEQNSRKYDMIIVDLFIDDTVPDQFL
                     EADFLMLCKVNLVKGGLVLFNWMTVTKAQTQNYKDYRDMIFNEIMDHPLEVKTKYNHI
                     LVGYSFPTISGS"
     gene            1896..>3187
                     /locus_tag="PKV31_09075"
     CDS             1896..>3187
                     /locus_tag="PKV31_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB93050.1"
                     /translation="MLRYFSILLLFLSIIPLYAQKQSFVSGQVMIMLQDNTGFEDFTR
                     ELYSNNRNVLIDNYKQLSKTIPIYLISSQSFEGDEKVWVSFIAKLSTVRFAQVNHYVT
                     LRNTVPQDPRYSDQWQWNNTGTGNGKIGADVSAEKAWDITTGGLTAAGDTIVVCVIDD
                     GIDLQHEDLAANIWYNNGEIPNNGIDDDGNGFVDDFNGWNFNTQNDNVSVGSHGVNVS
                     GMIGAVGNNNMGVTGINWNVKIMNIVLSDFTEAAIVECYDYPYQSRLAYNNSHGDKGA
                     FVVSVNMSNGIDYGNPDDAPIWCGFYDIMGEAGILNVAATSNQNINVDVDGDLPTGCT
                     SQYLISVGRTDKNDNYAPCGYGPISIDLGAPGVNIVTTRSRNRYTTTTGTSFSSPLVA
                     GLVGLMYSYPCDNIGQLMHDDYAAFALLVKDAILNGVD"
BASE COUNT          987 a          641 c          649 g          910 t
ORIGIN      
        1 aagttagata ataaatacta tgtaaccgaa tcagaaagaa tatttctgga agaaggtgcc
       61 attgtagaat gttgtaatat aaatacaaca accgggcctg tataccttgg caaaaattcc
      121 atgattatgg aaggggcgaa tatacgtgga ggtctggcac tttgtgaaga aagtatcgta
      181 aaaatgggtg caaagttata cggtgctagt acattcggac ctcactgcaa ggttggcgga
      241 gaagtcaata acagtatctt ccagggcttt tccaacaaag gccacgaagg ctatctcggc
      301 aacagtgtta tcggcgaatg gtgcaacttt ggtgcagata ccaatgcatc caatttgaaa
      361 aacacctaca aaaaggtcaa gatttacaac taccgacaaa atatttccat tgactccgga
      421 cttttattcc ttggtttgat catgggcgat cattctaagt ccggcatcaa tactatgttc
      481 aacaccggga ctgtggtagg cgtatttgcc aatatctttg gtacgggatt cccgcccggc
      541 catatccctt ctttctcgtg gggtggagct gaacatatgg aaccttatca gtttgaaaag
      601 gcaatagaag tagctgaagc agtcatgagc aggaaaaacc aacatcttac tcctgaatac
      661 aaggcaatct tacgccatat ttacgacaat acgcatcact aaatatattc atcgaattga
      721 aattacccgt caatacctgg accaaactcc taagttatct tgtaaaggtg cccgtgagca
      781 ggtctgaatc cataaatgac ggcgcactgt atgttgatct ggtcaaggga cgtctccagc
      841 tcagtacgga tcatgcagta tactcctttg atgacaaata cgacaacttt gtcaaagcct
      901 tgaaagccgt caaggcggat cttttccgta acaaatccat tcttgtcctg ggcggaggtt
      961 taggtagcat cccttatatt ttggagaaga accatcaatt tcaaggtgaa ctgaccatgg
     1021 tggaaaatga tcctgctgtg attagattat ttctggaata tagtctgcca agaatgaata
     1081 cccttattga catcagggaa tcggatgcca tcatctttat ggaacaaaat tcaaggaagt
     1141 acgacatgat aattgttgat ttgtttatcg acgatactgt accggatcaa tttcttgaag
     1201 cagatttcct tatgttgtgt aaagtcaacc tcgtcaaagg gggcctggtc ctattcaact
     1261 ggatgactgt tactaaagcg cagactcaaa attataagga ttacagggac atgattttca
     1321 atgaaataat ggatcatccc ttagaagtaa aaacaaaata caatcatatt cttgtcggtt
     1381 attctttccc cacaatttca ggcagttaac ccgcaattcc ccggtatttt atggttaaga
     1441 gccatcactg aggttgcctt aactctgata attatctcac tttgggattt ccgggaagta
     1501 taaattccaa aagccaataa tatcatcatc aacaatgcgc ccatccattt aattatcctt
     1561 gtattcacca tgcctcagga tttgaactac ccataccgat attctgtctc tatttactta
     1621 atgcactgta aaaaccacat gcttattctc aaattatcag acaaactaac cgagatctta
     1681 ttcaaaatat tgcactttga accaaaaaaa atcaaaattt aacactttta agtaagtcga
     1741 tataaggtat aactttggtt ttttggtaat agccgattga tcattaggat ttttcccaaa
     1801 ttgaaaaaat ttccaaaaac catgtatatt tgagagttta taaccataaa agtataaaat
     1861 ataggtcttt ggccacattc ataaaaacca gttctatgct tagatatttt tccattctac
     1921 ttctcttttt gtccataatt cctctgtatg cccaaaaaca atcctttgtc tccgggcaag
     1981 ttatgattat gcttcaggat aatactggat ttgaagattt tacccgggaa ttatattcca
     2041 ataatcggaa tgtgctcatc gataattata agcaattatc caagaccatc cccatctatc
     2101 tgatctcatc acagtcattc gaaggtgatg aaaaagtctg ggtttcattc atagccaagc
     2161 tttcaactgt cagattcgct caggtcaatc attacgtaac acttagaaac actgtcccgc
     2221 aggatccaag atatagtgat caatggcaat ggaataatac cggtaccgga aatggtaaaa
     2281 ttggtgcaga cgtaagcgcc gaaaaagcat gggacatcac aactggcggc cttactgccg
     2341 caggcgatac catcgtagta tgtgttattg acgatggtat tgaccttcag catgaagacc
     2401 ttgcagctaa catatggtac aacaatggcg aaatcccaaa taacggaatc gatgatgatg
     2461 gcaatggctt tgtggatgat ttcaatggct ggaattttaa tactcaaaat gacaacgtaa
     2521 gtgtaggcag tcacggggtg aatgtgagtg gaatgattgg agcggtagga aataataata
     2581 tgggagttac aggcatcaac tggaacgtaa aaattatgaa cattgtcctt tcagatttta
     2641 ctgaagccgc catcgtagag tgttatgact atccgtacca aagcagattg gcttataaca
     2701 actctcatgg agataaaggt gcctttgtgg tttcagtcaa tatgtccaat ggtatcgatt
     2761 atggcaatcc tgatgatgca ccgatttggt gtggcttcta tgatatcatg ggagaagccg
     2821 gaattttgaa tgtagccgcc acatccaatc aaaatatcaa tgtcgatgtg gacggagatc
     2881 tgccgaccgg ttgtaccagt caatacttga tatctgtagg gcgtaccgac aaaaacgaca
     2941 attatgcacc ctgcggctac ggcccgataa gcatcgatct gggtgccccg ggggtcaata
     3001 tcgttactac gagatcaaga aacagatata ctacaacaac aggaacttct ttttcttcgc
     3061 cattggtggc gggcctcgta ggcttaatgt atagttatcc ctgtgacaac ataggtcagt
     3121 tgatgcacga tgactatgct gcctttgcac tacttgtcaa ggatgccatc ctcaatgggg
     3181 tagataa
//
LOCUS       DAOQMC010000258         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_16871_length_3156_cov_4.269268, whole genome shotgun sequence.
ACCESSION   DAOQMC010000258 DAOQMC010000000
VERSION     DAOQMC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16871_length_3156_cov_4.269268"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..2618
                     /locus_tag="PKV31_09080"
     CDS             231..2618
                     /locus_tag="PKV31_09080"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB93051.1"
                     /translation="MEKLNVLGNAMYLAAHPDDENTRLISYFTHGKHFNTTYLSLTRG
                     DGGQNLIGTELGASLGVLRTEELLRARSIDGGNQWFTRANDFGFSKHPDETLQFWNEA
                     EILKDMVNAIRKFKPDIIVNRFTTRNPGETHGHHSASAILADKAILLAADDNYTIPGS
                     NYPAWQVTNMYFNTSYFFYKSREEFERADKSGLYKVDCGLYYPLVGMSNGEIAAQSRS
                     SHRCQGFGSAYSRGSSIEYLELVAGKAPSDFNNPFAEINSTWSRVKGGDAISDKVNSI
                     LRAYDYKHPENSIPALIEVSKMIDKLEDPFWRKIKSEEIKSIILDCAGFYFEAVTDSK
                     TVCPGDSMRINIEYINRASIPVSITKLTLLNNVVKNTNLALADNNNIKENYSVRVPHD
                     IPLTTPYWLQGNSSPMMYDVSDRDIIGRPENIYPLDGRLSIDVAGYAITIPIRILYKN
                     VEPAYGQRYGEVQVVPPVTVNIDRNCILAHPTGKTSLHCTVRGFVSDISARLYPDLPK
                     GWTSEPPYFDIGMKLSGESRDYKFMISGPAFAGIDSVGAHIEWKGKHYNRSFQEIRYD
                     HVPYRIINIANKVPVSIVAPPKSKELIGYIDGAGDLVFESLKAAGYNIERIPSDQLNE
                     SQLAKYRTILVGIRAFNVLENAAVLNQALSEYAANGGRVVVQYTTAGGMKVKQIGPYP
                     FSVSRTRVTDESADVHFLLPEHILVNKPNKITTDDFRYWVQERGLYFGDKYQDNYQAL
                     FGMHDKNENEARGSLLYAPVGKGSFIYTGLVFFRELPAGIPGAYRLMENLLYNPQ"
     gene            2731..>3156
                     /locus_tag="PKV31_09085"
     CDS             2731..>3156
                     /locus_tag="PKV31_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNB93052.1"
                     /translation="MRELDWVVLVATLLFITFYGIAKNLRNQTAESYVVGTRDMKWWA
                     ICISIMTTQASAVTFISTPGQGFVDGMRFVQFYFGLPLAMIVLSAFFVPLYYKMRVQT
                     AYEYLEHRFDLKTRSLTSVLFLIQRAMGAGLTIYAPAIVL"
BASE COUNT          880 a          622 c          746 g          908 t
ORIGIN      
        1 agcaggaaaa atattatttt gagaagtaaa gtaaacttag tattaaaaat gatgttaccc
       61 atgaagttct tttttaagtt ccgaaattga gtaatttttt gttttatttg agttaaaaag
      121 acaaatttat tttgaggcat ttcgtattta cttcattttt aatgtgtttt gcggcaattt
      181 tatccgcgca acaatttcaa ccaacttcag ccgaccggat atttttcaaa atggaaaaat
      241 tgaatgttct gggcaatgct atgtatctgg cagcccatcc ggatgatgaa aatacacgat
      301 tgattagtta ttttacccat gggaagcatt ttaatacaac ctacctttct ttgacaaggg
      361 gcgatggggg gcaaaatctt atcggtactg agttgggagc ttcacttggc gtgttgagga
      421 cggaagaatt acttcgagcc agatcaattg atggcggcaa tcaatggttt acccgcgcca
      481 atgacttcgg attcagtaag catcctgatg agacactgca attttggaat gaagccgaaa
      541 ttctcaagga tatggtcaat gccatcagaa aattcaaacc ggacatcatt gtcaaccgtt
      601 ttacgacccg gaaccccgga gaaacacacg gtcaccacag tgcttcggct attctggcgg
      661 ataaagcgat attactggct gcagacgaca actatacaat accgggttca aactatccgg
      721 catggcaggt gactaatatg tattttaata cttcctattt cttctacaag tcacgggaag
      781 agtttgaaag ggcagataaa agtggtttgt ataaggtcga ttgtggtctg tattatccat
      841 tggtcggtat gtccaatggt gagattgccg ctcagagccg tagttcccat cggtgtcagg
      901 gttttggcag tgcctattca cgcggatcgt ccattgaata ccttgagctg gttgccggaa
      961 aagctccttc agattttaac aatccctttg ctgaaataaa ttccacctgg tcaagagtaa
     1021 agggtggtga tgctatttca gataaggtca actctatctt aagggcctat gactataagc
     1081 atcccgaaaa ttcaatcccg gccctgatcg aggtgtcaaa aatgatcgat aagctggaag
     1141 atccattctg gaggaagatc aagtctgaag agatcaaatc cattattctc gattgcgccg
     1201 gattttattt tgaagctgtg acggatagta aaacggtctg cccgggagat tctatgagaa
     1261 ttaacataga atatatcaat agggcctcaa tacctgtgag tataactaaa ttgacccttt
     1321 tgaacaatgt tgtgaagaat accaatcttg ctcttgccga taataataac atcaaggaga
     1381 attactccgt ccgggtaccc catgacatcc cattgacaac gccttattgg ttgcagggca
     1441 attcctcgcc gatgatgtat gatgtcagcg atcgggacat cattggaaga ccggagaata
     1501 tatatccatt ggacggaagg ttgagtatcg atgtcgccgg atatgccatt acaattccca
     1561 taagaatatt gtataagaat gtcgagccgg cttatggaca gcgatacggt gaagttcagg
     1621 tggtgccgcc tgtcacagtg aatatcgacc ggaattgtat ccttgcacat cccacgggca
     1681 aaacgtcact acattgtacg gtcagggggt tcgtgtctga tatcagtgcg agattatatc
     1741 ccgacttacc aaaaggatgg acatcggagc cgccctattt tgatattggc atgaaactca
     1801 gtggagagtc aagggactat aaattcatga tttcaggtcc tgcatttgcc ggtattgatt
     1861 ccgttggtgc tcacatagaa tggaagggta agcattataa tcgcagtttt caggagatca
     1921 ggtatgatca tgtcccttac cggattatta atattgcaaa caaggttccg gtttccatcg
     1981 tggccccgcc aaaaagcaag gagttgattg gctatatcga cggagccggt gatttggttt
     2041 ttgaatcgtt aaaggcagca ggctacaata ttgaaagaat tccttcagac cagttgaatg
     2101 aatcccaatt ggcaaaatac agaactattt tagtgggcat ccgcgcattc aatgtacttg
     2161 aaaatgccgc tgtgctcaat caagcattga gtgagtatgc tgcaaatggt ggcagggtag
     2221 tggttcagta tacaacagca ggtggtatga aggtcaaaca aatcggacct tatcctttta
     2281 gtgtgagtcg aaccagagtt actgatgaga gcgctgatgt tcattttctg cttccggaac
     2341 atatccttgt taataagccg aataaaatca ctacggatga cttcaggtac tgggttcagg
     2401 aacgaggtct ttattttgga gataaatacc aggataatta tcaggcattg ttcggaatgc
     2461 atgacaaaaa tgaaaatgaa gccaggggaa gtttacttta tgcacctgtt ggcaaaggtt
     2521 catttatata taccggcctg gttttcttta gagagcttcc tgccggtatc cccggagcat
     2581 accgattgat ggagaatttg ttatacaacc ctcagtgatc aggatgaagg aagatcaaac
     2641 caactggaac aaggcgtaca tatgggtgtt tgctgtgatg gtgttcacca tatttgtttt
     2701 ttggctgatt caaataaatt ataaccacgc atgagggagc tggactgggt agttttggtt
     2761 gcgaccttgt tgtttatcac attttacggt attgcgaaga atcttcggaa ccaaacagcc
     2821 gagtcgtacg tcgttggcac ccgggatatg aagtggtggg ctatctgcat atcgatcatg
     2881 acgacacagg ccagtgcagt taccttcata tctacgcccg gccagggttt tgtcgacggc
     2941 atgcgctttg tgcagttcta ttttggcctg ccgcttgcaa tgattgtttt gtcggctttc
     3001 tttgtgccac tttattacaa gatgcgcgta cagacagcct atgaatattt ggaacatcgc
     3061 ttcgacctga agaccagaag cctcacgtct gtgttgtttt tgatacaaag ggccatgggc
     3121 gcaggcctga ccatatatgc acctgcaatt gtcctg
//
LOCUS       DAOQMC010000259         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_16939_length_3149_cov_4.991273, whole genome shotgun sequence.
ACCESSION   DAOQMC010000259 DAOQMC010000000
VERSION     DAOQMC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16939_length_3149_cov_4.991273"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKV31_09090"
     CDS             <1..904
                     /locus_tag="PKV31_09090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB93053.1"
                     /translation="IGAVIGSWLPYALSNWFGVSSEAASNEVPYHIILSFIIGAMVMI
                     GAVMVTIFSVKEFTPEQITQFEQEEGVSTEQPSKGLVSIFSDFAQMPKTMRQLAWVQF
                     FSWFALFGMWVFSTPAIAHHIYGTPLVEYNELTETQKKIYQEAGDWVGIIFGVYNGVS
                     AIFAFFLPWIAKRIGRKKTHALALGIGGLGLISIYFFHSPGMLILSMIGVGIAWASIL
                     AMPYAILAGSIPAKKMGVYMGIFNFFIVLPQIVNAIIGGPIVKYLYGGNAIFAIVTSG
                     IAMIIAGICVFKVYDVDETLLPKS"
     gene            1388..2347
                     /locus_tag="PKV31_09095"
     CDS             1388..2347
                     /locus_tag="PKV31_09095"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNB93054.1"
                     /translation="MVFLDFEKPIADLYEQLNKLRELDNQGDINMEEMVESLEKKIEI
                     EREKLYKNLSAWQRVQLSRHPERPYTLDYISRIFTDFIELHGDRNVHDDKAIVGGMAK
                     LDGKPVMIIGHQKGHNTKERQFRSFGMANPEGYRKALRLMRLAEKFNMPVICFIDTPG
                     AYPGLEAEDRGQAEAIARNIYEMSHLKTNIICYIIGEGASGGALGIGVGDRVFMLENT
                     WYTVISPENCSTILWRSWDYKVKAAEELKLTPEHMFNFNLVDGIVPEPLGGAHSDYDE
                     MAATLKKHIATNLKELAKEGNKNMVRKRIEKYRNMGKFHIKEG"
     gene            2373..2888
                     /locus_tag="PKV31_09100"
     CDS             2373..2888
                     /locus_tag="PKV31_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93055.1"
                     /translation="MSLHTSLSRFFLKRFLRERKDTSSTFKLAENNYQSLLLVFQNQT
                     DVARIVQSIKTIAGRTFSRIDYIIFTDNKSENAPEKGILSTRLVGMQGKFSAKGTQPV
                     HSSAYDIIVDMCDVTELNLIHYLLKLSPQLFVRFNKEKGIFYNFIVPTGQKDLLARFE
                     QFYRIFQTLSK"
     gene            2885..>3149
                     /locus_tag="PKV31_09105"
     CDS             2885..>3149
                     /locus_tag="PKV31_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNB93056.1"
                     /translation="MNNKARFQGTGVAVVTPFKSGQVDYEALTNVLNHVVDGGVDMIV
                     CLGTTGEATALSDHEQQEILAHTIEVVAGRKPIVYGPFGSNNTA"
BASE COUNT          956 a          672 c          682 g          839 t
ORIGIN      
        1 cattggagcc gtgataggct cctggcttcc ctatgcgctg agcaactggt ttggtgtatc
       61 ctctgaggca gcttcaaacg aggtgcctta tcacattatc ctttccttta tcataggtgc
      121 aatggtcatg atcggagctg tgatggttac catattttca gtaaaggaat tcacgccgga
      181 gcaaataacc caatttgaac aggaagaagg agtctcaaca gaacaaccat ccaaaggttt
      241 ggtttctatc ttttccgatt ttgcccagat gccgaaaacc atgaggcaat tagcctgggt
      301 acaattcttt tcatggtttg cactattcgg catgtgggta ttcagtacgc cggccattgc
      361 gcaccatata tacggaaccc ctttggtcga atacaatgag ttgaccgaaa ctcagaagaa
      421 gatatatcag gaagccggag actgggtggg aatcatattc ggcgtataca atggagtctc
      481 ggcaatattc gccttttttc ttccatggat tgccaaaagg attggtcgca aaaagacaca
      541 tgctcttgca cttggcatag gtggcctcgg attgatttcc atttatttct tccattcacc
      601 cgggatgctt attctttcta tgattggtgt gggtattgcc tgggccagca tcctggctat
      661 gccttatgcc atactcgcag gctcaatccc tgccaagaag atgggagtgt atatgggaat
      721 cttcaacttc ttcatcgtac tgccccagat agtcaacgcc atcattggcg gtcctatcgt
      781 caaatatctc tatggtggca atgcaatctt cgccattgtg accagcggga tagccatgat
      841 cattgcggga atttgcgtct tcaaggtgta tgatgtggac gaaactttat tgcctaaatc
      901 ctgaccaata ttaataaatc tgccaggcaa aagacttcat tataaaagtt aataaccata
      961 aatctaaaaa gataacggat atcagaaaaa gaaatcggca gtgaaaaaaa gcgaattagg
     1021 tagatgatta atatgtgaga ggtgcgtgtt tagaacccat tcgcaaatat tatgattgaa
     1081 aaatggtcag atttaggcga aattattttc gttgaattta cagactcttg ttccaaccat
     1141 gttgtcaaat attaatttca tacctacttc catttgccca aattataaag tattattcca
     1201 gtccaggaag cctacatccc gaatcatgcc caacacaccc cattataaat ccaatggcat
     1261 acaaaaacct tataatatat aaatcactat ttctctacac gagtcgaaaa gctttgtcga
     1321 tactgaatca taattgaaat tggtttattg aataaaactt atatttgcag acaaaatatt
     1381 tgttgccatg gtatttttgg actttgagaa accgatagca gacctatacg aacagctaaa
     1441 taagctgcgg gaattagaca atcagggtga catcaatatg gaggaaatgg ttgaatccct
     1501 tgagaagaaa atagaaatcg agcgggagaa gctatacaaa aacctctcag catggcagcg
     1561 tgtccagctc tccaggcatc cggaaaggcc ctataccctt gattatatat cgcgaatatt
     1621 cactgacttt attgaactac atggcgatag aaacgtgcat gacgacaaag ccattgtcgg
     1681 aggaatggct aagttggatg gtaagcctgt catgattatc ggtcaccaga aaggtcataa
     1741 taccaaggaa aggcaattca gaagtttcgg catggccaat ccggaagggt atcgcaaggc
     1801 tttacgcctc atgcgtttgg ctgaaaaatt caatatgccg gtaatctgtt tcattgacac
     1861 accgggagct taccccggac ttgaagctga agacaggggt caggccgaag ccatagcccg
     1921 caacatatac gaaatgtccc acctgaagac caatatcatc tgttacatca ttggcgaggg
     1981 agcctccggg ggtgcattgg gcatcggagt cggcgacaga gtctttatgc ttgaaaatac
     2041 atggtacaca gttatttccc ctgaaaactg ctcgaccatc ctatggagaa gttgggatta
     2101 taaagtaaag gccgctgaag aactcaaact cacacctgaa cacatgttta atttcaacct
     2161 ggtggacggc atcgttcctg aacctctggg cggagcacac tctgactacg atgagatggc
     2221 ggcaacactg aaaaaacaca tagccaccaa cctgaaggaa ttagccaagg aaggtaataa
     2281 gaacatggtc cggaagcgga ttgagaagta ccgcaacatg ggtaagtttc atattaagga
     2341 aggttgatta tttcgattaa tatctacagc acttgagcct ccacacgtcg ctttcccgtt
     2401 ttttcctgaa aagattcctt agggaaagaa aagatacatc atcaacattc aagctcgctg
     2461 aaaataatta tcaatccctg ttgcttgtat tccaaaatca aacggatgtc gcccgaattg
     2521 tacaatccat aaaaacaatt gccggcagga cgttttcccg gattgattac atcatattta
     2581 ctgacaataa gtctgaaaat gccccggaga aaggaatcct aagtacaaga cttgtaggca
     2641 tgcagggcaa attctctgcc aagggcacac aaccggtgca ttcatcagcc tatgatatca
     2701 ttgtcgatat gtgtgatgtc actgaactga acctgattca ctatttgctg aaattatcgc
     2761 ctcaactttt tgtaagattt aataaagaga aaggaatttt ttataacttt atcgttccaa
     2821 ccggacaaaa agaccttctg gcccggtttg aacaatttta caggatattt caaaccttat
     2881 caaagtgaat aacaaagcac gtttccaagg tacaggcgta gcggttgtaa cccctttcaa
     2941 gtcaggacaa gtcgattacg aagccctcac caatgtgttg aatcatgtgg tcgatggcgg
     3001 cgtggatatg atcgtgtgtt tgggcacaac cggagaagcc acggctctca gtgatcatga
     3061 acaacaagag atccttgccc acactattga agtggtcgcc ggaagaaaac ctatcgtata
     3121 tgggcctttt ggaagcaaca atacagctg
//
LOCUS       DAOQMC010000260         3097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_17457_length_3097_cov_5.834320, whole genome shotgun sequence.
ACCESSION   DAOQMC010000260 DAOQMC010000000
VERSION     DAOQMC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17457_length_3097_cov_5.834320"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PKV31_09110"
     CDS             complement(<1..1180)
                     /locus_tag="PKV31_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93057.1"
                     /translation="MKRITLAGCIMVLTVSCFAQKNLKDTINEKEQQVSEVQNDNQNL
                     NQFKVSGYIQAQIQSGSKDATLRVGNKNTDPDHRFNRFGIRRGRVKLTYTKNIVSGVF
                     QLDITERAISLKDAYISIQDPHWGIYSIRAGVFNRPFGYEVAYSSSRRESPERALVNT
                     ILFPNERDLGAMLTLQGPKASMWNFFKLEAAIIAGNSIRIDMDNRKDFLGRVSFNKAF
                     GEKINLSGGLSYYLGGVYQGSYNVYTMKGRSFLLSHDTTNLGAFTKREYCGVDLQFEA
                     TTRLGMTRLYGEFLTGVQPGDEQDSQSPNYSKLPTTDTYIRPFRGGYITFVQDVGHMP
                     LAVIVKYDIYDPNTDVADNDIGLNGTGTGDVAFENIGCGLMWHIRDDLKLTGYYDHII
                     N"
     gene            complement(1891..2211)
                     /locus_tag="PKV31_09115"
     CDS             complement(1891..2211)
                     /locus_tag="PKV31_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93058.1"
                     /translation="MPHNDWSVLHFPDHAESMEDDVRFHFRDPEELKYADPEDIVEED
                     NGFPLHISLTCIALYLFATGMIFLLVQNWNQDRTPYFVRIFISIISATYLIFRFHKRP
                     VLKS"
     gene            complement(2406..2476)
                     /locus_tag="PKV31_09120"
     tRNA            complement(2406..2476)
                     /locus_tag="PKV31_09120"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2442..2444),aa:Cys,seq:gca)
     gene            2640..>3097
                     /locus_tag="PKV31_09125"
     CDS             2640..>3097
                     /locus_tag="PKV31_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93059.1"
                     /translation="MTNDRLIQKYIFLFLIVGLGVTIVSNLSAFITAALGASIFYILF
                     RRQMSRLVEKFKIGKTVSALIIILLSFFIVVLPFTMMGLLIANKVIYYINQPQAFNNI
                     LDLVLEKIKLLPFEIKVGEMISRMTSLVGSLLTGVLNNIFGVLTTIVVMY"
BASE COUNT          926 a          642 c          604 g          925 t
ORIGIN      
        1 cgttgatgat gtgatcataa tatccagtca gcttcaagtc atcgcggatg tgccacataa
       61 ggccgcaacc aatgttctca aaagccacat ctcccgtacc agtgccattc aggccgatgt
      121 cattgtcggc aacatcagtg ttggggtcgt aaatatcata tttgacaatc actgcaagcg
      181 gcatgtgccc cacatcctgt acaaatgtga tataaccacc ccggaaaggt cttatgtagg
      241 tatcggtggt aggcaatttt gaataattcg gactttggga atcttgttca tctcccggtt
      301 gtacacctgt gagaaactca ccatacaacc ttgtcatacc gagcctggtg gtagcttcaa
      361 actgtagatc cacaccacag tattcccttt tggtaaaggc tccaaggttg gtggtatcat
      421 gactcagcag aaatgagcgc cctttcatgg tatacacatt gtagctacct tgatataccc
      481 cacccaaata ataggacaag cctccactca ggttgatctt ttcgccgaag gctttattaa
      541 aggacaccct ccctaaaaaa tccttccgat tatccatgtc gatccgtatg ctgttgcccg
      601 cgattattgc tgcctccagt ttgaaaaaat tccacataga agccttgggt ccttgaaggg
      661 tcagcatcgc ccccagatct ctttcgttag gaaacaggat tgtgttgaca agtgcccttt
      721 ccggagattc ccgcctggaa gaagaatagg caacctcata cccgaagggc cgattgaata
      781 caccggccct gattgaataa atgccccaat gaggatcttg tatggatata tatgcatcct
      841 tcaatgagat tgccctttca gtgatgtcaa gctgaaagac accggaaaca atgttttttg
      901 tataggtcaa tttaactctg cctcttctga tgccaaaacg attaaaacga tgatcaggat
      961 ctgtattttt attgcctacc cgaagcgttg cgtccttgct tccgctttgt atttgtgcct
     1021 ggatgtatcc ggaaaccttg aactgattta aattttgatt atcattctgc acttctgata
     1081 cctgctgctc tttttcattg attgtgtctt ttaaattctt ttgagcgaag cacgagacag
     1141 taagcaccat tatacaaccg gccaaagtaa ttctcttcat cagaatataa atttgattgt
     1201 atgcaaagat tggaatacga tatgaccaca tgatccaagg atggttacga ttagtttaaa
     1261 gtcgaggcga aaatattcaa ttactttaaa atcttaaagt tacaaatttg ctttgatgtc
     1321 tattgacaaa gttttatgat caaattttaa tataatcttg ccggtatttt ccttgtaaaa
     1381 atgcttgaat attggcattc aatccattgc gttcctttct gatgataaag gtgacttgcc
     1441 tgcagagatt gaaaaccagg gtaatgaagg atcctattta tcggtcgaac ctttaaaata
     1501 aaagaccaaa tcatgagtca ctttgatgaa ttcaatgcct gtcaagttga gacaaataac
     1561 aggaagtgac aaagtctgag tgacaggaaa accgggctga cctgcgtgaa cattcatttg
     1621 gagtattgaa ccacccaaac ggttttccac aataagatag caattatcat tcgttggatt
     1681 ttcaaaagcc gaatagcgtt atgcctggtc atttccgaaa cgtgagccta tgatataaaa
     1741 gatgtgatgt tgaatccggc acataacgat tggaatagct atctccggct atcagatatt
     1801 cagttgaatt aacttctcat gaatattgca ttgaacgtgc ctgaaaagaa ctatcctgac
     1861 tagcagaaag agaaacatta cgaatctgaa tcaggatttc aaaacaggtc gcttgtggaa
     1921 cctaaaaatc agataagtcg ctgaaattat agatatgaaa attctgacaa aataaggtgt
     1981 cctgtcctga ttccaatttt ggaccaaaag aaatatcata cctgttgcaa ataggtacaa
     2041 cgcaatacag gtcaggctta tatgcaatgg aaaaccattg tcttcttcca cgatgtcttc
     2101 cggatcggca tatttgagtt cttccggatc ccggaaatga aacctgacat cgtcttccat
     2161 agattcagca tgatccggaa aatgcaaaac agaccagtca ttgtgaggca taagcctgat
     2221 ttcttttgaa atttctcccg gcaaaaccat ggttggaaaa aagaattaaa gttgaacatt
     2281 catttaaaat cccattggta gttttcaaaa aaatattcag aaggccttgt ctcaattacg
     2341 gtaaggacca caattcagtt cttactgtat tccaatcaaa taagggggag tatatcaaaa
     2401 gaaagaggtc ggtggcgggt tcgaaccgcc gtagaaggtt ttgcagacct ttgcctaacc
     2461 gctcggccaa ccgaccctgt ttcgtaagcg gtgcaaatat aattcttttc aaacaaatac
     2521 caagagaggc tgttcatatt ttcaaatttt taaataaata aaagtatgat atttactgat
     2581 tgccaaatat ttaaaatcca tcacaatttt tatacatttg attcttaaat gaattaaaca
     2641 tgacgaacga caggctcatc caaaaataca ttttcctatt tctgatcgtt gggctcgggg
     2701 tgacaattgt gtcaaacctg tccgctttca taacagcagc cttaggtgca agtatctttt
     2761 atattctatt tagacggcag atgagccgtc tggtggaaaa attcaagatc ggcaagactg
     2821 tttcagccct tattatcatt cttttatcat tttttattgt agtcctgcca tttacgatga
     2881 tgggtcttct gattgccaac aaagtcatct attatatcaa tcaacctcag gccttcaaca
     2941 atatcctgga tttagtcctc gagaagatca agttgttgcc gtttgaaatc aaggttggcg
     3001 agatgatctc gcggatgact tcattggtag gctcattgtt gacaggcgtc cttaacaaca
     3061 tattcggtgt acttacaacc atcgtggtca tgtattt
//
LOCUS       DAOQMC010000261         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_17547_length_3089_cov_4.671061, whole genome shotgun sequence.
ACCESSION   DAOQMC010000261 DAOQMC010000000
VERSION     DAOQMC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17547_length_3089_cov_4.671061"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PKV31_09130"
     CDS             complement(<1..757)
                     /locus_tag="PKV31_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNB93060.1"
                     /translation="MTALAIIGCILLVGLVIYQIARINEIIKRIKGEEKAFLQSNNNT
                     AVAILVFGFLLLFLTIYTSYHYKNMMFGYGPMLSASEHGTLIDKSMHIMLILTGIVYI
                     ITHILLFYFAYRYRYRKGKKAWYYPDNTKLEMVWTLTPFFVLAFLAIDGLMVWNKMMA
                     DIKPDEKHIEVEVTGMQFAWIVRYPGIDNILGTRDFKLTSGTNDLGQDWNDPKNWDDF
                     KPDEIVLPKGKKVRIRILSRDVLHSFFLPHFRVK"
     gene            complement(791..2065)
                     /locus_tag="PKV31_09135"
     CDS             complement(791..2065)
                     /locus_tag="PKV31_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93061.1"
                     /translation="MDQFIFKSKYKNTLIAFMVIGLISMVITWFQDDALHTRFWSNYL
                     HNTMYFTGIAFAALMLMTMGILTYGSWWVAFKRLWEAYSMFLITGLILMLVVVGGVWG
                     HYHHLYHWTDAEDVANDLILKGKSGFLNPYWYTLGTVIVIGAWAYFAIKLRQLSKEQD
                     NNNGFDDIKEYKRMKVLSSIFLPIAGFSSAAMIWQWIMSLDAHWYSTLFAWYATASWV
                     VACFAFTALLIIFLKGQGFINFVTVEHLHDVGKFVFAITVFWTYLWFSQFMLIWYGNN
                     GEETTYFNRQMQQYPVLFWGNLVINFVLPFLVLLRNDTKRKIGSMVFVCILVIFGHWW
                     DFFLMIKPGTLHTAHEALAKMEGGHGAVAEGAEHASNFVSGFTLPGLLEIGTMLGFFG
                     LFVYVTFVQLGKLSLKPKHDPLLPESLHHHVQ"
     gene            complement(2120..2920)
                     /locus_tag="PKV31_09140"
     CDS             complement(2120..2920)
                     /locus_tag="PKV31_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB93062.1"
                     /translation="MKYNRYLIFSGLAFIAASLSLESCAPAGGNKPGHEYMQDMGHSI
                     AYEANMVDLFHYNRWDMNGYIKLAQPRNPVMGTIPRGFVGLAKYSNSEIPASVEAEFA
                     NETVNGSVPFYYGDTEEDRKRAENDIKLNPYPITKENLAHGKELYDIYCGICHGEKGD
                     GSGYLVRDDGKYPAQPAILTSDEFIAASNGRFYFAIMHGKNVMGSYSDKLSYKERWQV
                     IQYIRSLQAKTKGLKYDETENTLNNTDFIASAKKAMPAVADTMPTVAN"
BASE COUNT          990 a          682 c          517 g          900 t
ORIGIN      
        1 tctttaccct gaagtgagga agaaagaaac tgtgcaatac atcacgggac aaaattctga
       61 ttcttacttt tttccctttg ggtaaaacta tttcatcagg cttgaaatca tcccaatttt
      121 tgggatcatt ccaatcctga ccaagatcat tggttccgga agttaattta aaatcccttg
      181 tccctaatat attatcaata cccggatacc taacaatcca tgcaaattgc attcctgtaa
      241 cctcgacctc tatatgcttt tcatccggct taatatctgc catcatctta ttccacacca
      301 ttaaaccatc aatggctaaa aatgcgagta caaagaaggg ggtcaaagtc cacaccattt
      361 cgagcttagt gttatcggga taataccaag cctttttacc tttcctatat cgatatctgt
      421 aagcaaagta aaataacagg atatgggtaa tgatatatac aatcccggtt aggatcaaca
      481 tgatatgcat ggatttatca atgagtgttc catgctcaga agcagaaagc attggtccat
      541 aaccaaacat catgttctta taatgatacg atgtgtagat cgtaaggaat agcaacaaga
      601 aaccgaatac caatattgca actgctgtat tgttattgga ttggaggaaa gctttctcct
      661 cacctttaat tctcttaata atctcattga ttctcgctat ctgatagata acaagcccaa
      721 caagcaaaat acatccaatt attgctaacg ctgtcattga ttatttaatg ttaaattatt
      781 gtcaaaaagt ttattgaaca tgatgatgta aactctcagg cagcagagga tcatgcttgg
      841 gtttaagcga caatttaccc aactgtacaa aagttacata aacaaatagt ccaaagaatc
      901 ctaacatagt tccgatctcc aacaaacccg gaagagtaaa gccggagaca aaattgcttg
      961 catgttcggc accctcagca acagctccat ggccgccctc cattttggca agagcttcat
     1021 gagcagtatg cagagttccc ggcttaatca tcaggaagaa atcccaccaa tgaccaaata
     1081 taaccagtat acaaacaaac accatcgatc ctattttccg ctttgtatca tttcttaata
     1141 acaccaggaa aggaagtaca aagttgatga caaggttacc ccagaaaagt acgggatatt
     1201 gttgcatctg cctgttgaaa taagtcgttt cctctccatt gttaccgtac cagatcaaca
     1261 tgaattggct gaaccacaaa taagtccaga aaacagtaat tgcaaaaaca aactttccca
     1321 catcgtgcag atgttctacc gtaacaaaat tgataaaacc ttgtcccttt aggaaaatga
     1381 tcaacaaggc tgtaaacgca aagcatgcta ctacccatga agctgtagcg taccatgcaa
     1441 ataaggttga ataccaatgt gcgtccaatg acataatcca ttgccaaatc attgcagcac
     1501 ttgaaaatcc tgcgatcggc agaaatatac tggatagcac tttcatgcgc ttatattcct
     1561 tgatatcatc aaagccatta ttgttatcct gctctttgga cagttgtctg agcttgattg
     1621 caaaataagc ccatgcacca ataacaataa cagtccccaa agtataccaa tatggattaa
     1681 gaaatccgga tttacctttg aggatcaaat cattggcaac atcttctgca tcggtccaat
     1741 gataaagatg gtgataatga ccccatacgc ctcctaccac aacaagcatc aatatcaagc
     1801 cggtaatcag gaacatcgaa taagcttccc ataaccgctt gaaagctacc caccaactac
     1861 catatgtaag aatacccatg gtcataagca tcagagcagc aaaggcaatt ccggtaaaat
     1921 acattgtatt atgcaaatag ttagaccaga atctggtatg cagagcatca tcctgaaacc
     1981 atgtaataac catgcttatc agtccaatga ccataaaggc aatcagtgta tttttgtatt
     2041 ttgatttgaa tatgaattga tccatcctca attaattttt tgttatcctc gaatataact
     2101 tattactgtt ttcttttcac taattggcaa cagtgggcat agtatcagct accgcaggca
     2161 tcgctttctt tgctgaagct ataaaatccg tattgttcaa cgtgttttct gtctcatcat
     2221 atttcaggcc ttttgtcttt gcctgcaaag atctgatgta ttgaatcacc tgccatcttt
     2281 ccttgtaaga caatttatcg gaataagaac ccatcacatt tttaccatgc atgatagcaa
     2341 aataaaatct tccattcgat gcagcaatga attcatcact ggttaggatt gcaggttgcg
     2401 caggatactt accatcatct cttacaagat atccggagcc atcccctttt tcaccatgac
     2461 aaattccgca gtagatatca tacaactctt taccatgagc aagattttct ttggtgatgg
     2521 gataaggatt caatttgata tcattttcgg cccgcttcct atcttcctct gtatcaccat
     2581 aatagaaagg aactgaacca ttgacggttt cattggcaaa ttcagcttca acagatgcag
     2641 ggatctcact attactgtat ttggcaagcc caacaaatcc tcttggaatt gttcccatca
     2701 caggatttcg aggctgagcc aatttgatat agccattcat gtcccaccga ttatagtgga
     2761 acaaatcaac catattagcc tcataggcga tggaatggcc catatcctgc atatattcat
     2821 ggcctggttt gttacctcct gcaggagcac aactttcaag cgacaagcta gctgcaatga
     2881 atgccagtcc ggaaaaaatc aaatatctat tatatttcat cttcaaaaga atgattcttt
     2941 ttattaaata aacttcgtgt caaccaaaga ggcaccatgc tttgagaaga aagctcgtag
     3001 gccggatttc tgttcatcgg atgcatcact tgtatcaaat gagatacaga atttatcatc
     3061 tgtaattctg tcatgtaatg tctttgcct
//
LOCUS       DAOQMC010000262         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_17629_length_3081_cov_5.112690, whole genome shotgun sequence.
ACCESSION   DAOQMC010000262 DAOQMC010000000
VERSION     DAOQMC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17629_length_3081_cov_5.112690"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1214
                     /locus_tag="PKV31_09145"
     CDS             315..1214
                     /locus_tag="PKV31_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93063.1"
                     /translation="MESIELKIKHFNSLLGSETSIYAMAQNTENNGFFTGGGSGEIVL
                     WPNFLGEEGLLYARTESPVLCLHADAVDNLLLIGTLDGQLNWIDLNKKSTVKRQKWMK
                     GGLYAIQSDEYSYYISGHGGIVTRISRDAKQATGGLQVSGIRCRALTQNSQFLISGTS
                     EGKIYMFDKLRFNNPIISSHKHQGSVFDIIQIENNYISCGKDGKLFKWDTALKKVDEV
                     QAHNSTINTLAPIGNSGLFATGCRDGSIRIWDSHDFELLKVIDLFLHSGHLRSVNKLI
                     WVDHQNLLMSCSDDRQIKMWKVQ"
     gene            complement(1357..2226)
                     /locus_tag="PKV31_09150"
     CDS             complement(1357..2226)
                     /locus_tag="PKV31_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNB93064.1"
                     /translation="MNSKLILSIAIFLFSFFNMSRAQVNQVKNIQLQLEGMVLDTFTL
                     EIRGSEVYINGRIADSSHVKYLLVKKDIATGNKIISEHGIEYQAEMKSAPVQIVEPPY
                     KGSPWLGLVTVQDGKGIKVVEVVKNGPASKTRILPGDYISTIDELKINNLDQLSDLVN
                     AKSPGEKIEIKLIRDETVKIVPVIIGDKNSGEIKRSYSKVDQPQMNGGRQIGMQLKLT
                     QDSKYWKVVSITSGSRAQSAGLNVNDIILEMNGVALKPGTDLAELMSEIGAGTEILLK
                     VKRAGKVLNIKMP"
     gene            2735..>3081
                     /locus_tag="PKV31_09155"
     CDS             2735..>3081
                     /locus_tag="PKV31_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB93065.1"
                     /translation="MFWKELVRQMYDIGIGSLVIVALICIFIGAVTAIQFNYQLDGTL
                     VPKYYIGYIVRDITLIEMAPTITCLVLAGKVGSNMASELGGMRQKEHIDAMDIMGVNT
                     YNFLIGPKVLGGVL"
BASE COUNT          948 a          668 c          546 g          919 t
ORIGIN      
        1 ctatgagttt gaaaataaat gacgccactt tgttacaatt cccggtaact atatcttctt
       61 cttccgcact aacactgacc aatcggctat tacttttcta cctacttcag ttcaaccttc
      121 ctcctcacac cacatcctga tctaccaata ttcctatcaa cccggaaaat gccagtcatc
      181 aagaatcatg gatttaaata aatttacgca cttccataaa caatacccgc catttagcct
      241 ttctaaaagc ataatcgatg attaaatcta tctggtagtc atacacaaaa ttccttgaaa
      301 atatttttgt cacaatggaa agtatcgaat tgaaaatcaa gcattttaat agcttattag
      361 gcagtgaaac atctatttat gctatggctc aaaataccga aaataatggc tttttcaccg
      421 gaggtggaag tggtgagatc gtcctttggc cgaacttcct cggtgaggag ggattgttat
      481 atgccaggac ggagagccct gtattatgcc tgcatgctga tgctgtcgac aatctacttc
      541 ttattggaac ccttgacggt caacttaact ggatcgatct gaacaaaaag tccactgtca
      601 aaagacagaa atggatgaaa ggcggattat atgccataca aagcgatgaa tatagttact
      661 atatctccgg acatgggggc attgtcactc gcataagcag agatgctaaa caggcaaccg
      721 gtggcttgca ggtttcaggt atcagatgtc gtgctttgac ccaaaatagt caatttctga
      781 ttagtggaac ttcagaagga aaaatataca tgtttgataa actccgtttt aataatccaa
      841 tcatcagttc ccacaaacat caggggagtg tcttcgatat catccagatt gaaaataact
      901 atatcagttg tggcaaggat ggcaagctat tcaaatggga tactgcgctt aaaaaagtgg
      961 atgaagtgca ggctcataac tccacaatca atacattggc accaattggt aatagcggat
     1021 tatttgctac gggttgccgc gatggaagta taagaatatg ggattcgcac gactttgaac
     1081 tactcaaagt catcgatttg ttcttacatt ccggacatct gcgctcagtg aataaattga
     1141 tctgggttga tcaccagaac ttattgatga gttgcagtga tgacaggcaa atcaaaatgt
     1201 ggaaggtgca ataagaacaa atcccttaca atttggcaaa cgcttaacta cagtacttcc
     1261 tgaataaaat tccgcctccg gtaattctac agattaccca atcttaatca ttttgcaaga
     1321 gacaaaaaac cggtgtctaa cccaatcatc taattatcac ggcatcttga tattcaagac
     1381 cttacccgct cgctttacct tgagaagtat ctccgtaccg gcgcctatct cactcatcaa
     1441 ctccgccaaa tcagtacctg gctttagcgc tacaccattc atctcgagaa tgatgtcatt
     1501 tacattcagg cccgcacttt gtgcccttga acctgaagta atcgacacaa ccttccaata
     1561 tttactatct tgagttagtt taagttgcat ccctatctgc ctcccaccat tcatttgcgg
     1621 ctgatcgacc ttcgagtaag atctcttaat ctcaccgcta ttcttatcac caattattac
     1681 cggtacgatc ttgaccgttt catcccgaat taatttgatt tcaatctttt ctcccggcga
     1741 tttcgcattc acaagatcgc tcagctgatc taagttattg attttcaatt catcaatagt
     1801 agagatatag tcgcccggca gaatcctggt tttggaagcc ggcccgttct ttaccacttc
     1861 caccactttt attccctttc cgtcctgcac agtaaccaaa cccagccagg gcgaaccctt
     1921 gtatggcggt tcaactatct gtaccggagc gcttttcatt tcagcctgat actcaatacc
     1981 atgttctgaa ataatcttat taccggtagc gatatctttc tttacaagta aatatttaac
     2041 atgtgaagaa tctgctatcc taccattgat ataaacctca cttccccgaa tctcaagggt
     2101 gaaggtatcc aataccattc cttccaattg cagctggata tttttcacct gattgacctg
     2161 ggcccggctc atattgaaaa aactaaagag gaagatggcg attgacaaga tcagtttaga
     2221 gttcataata actaaatttt aatgatttaa aatgttttca ataaaaacgc acagcgactt
     2281 tactccacta aagtaccata atctgggata caatccttta aaaaatagtt aaatttttaa
     2341 acatggcaaa agcgaaaatc cataagcaca ttgagctatg tattacaact tgtcttacaa
     2401 cacattatgg ccaatgccct ctatttttac caaataactt tgaaaggccg ccattctatt
     2461 aaagttatca tttgtttcat tgattaatta tctaatgtga acttccggtt aaatctcgga
     2521 accagttaaa tatttttata gatatgattt tgtaaatcag accattatat ttaaatttaa
     2581 aaaatataat aaaattatta tatatgtaat taaaaatgaa actatattta cccgataatt
     2641 cttgaaccaa acaatgttca aaaatccgct ataccattta ggaaggtact taaccatgat
     2701 gcgactggta ataagccggc ccaataggtt cagtatgttt tggaaggaat tggtccgtca
     2761 gatgtatgat atagggattg gctccctggt tatcgttgcg ttgatatgca tattcatagg
     2821 cgctgtgacg gcaattcaat ttaattacca gctggatggc actctggtcc ctaaatatta
     2881 tatcggctat atcgtaaggg acataacact tatcgaaatg gcaccaacca tcacctgtct
     2941 tgttctggca ggcaaggtag gttcaaacat ggcatcggaa ctgggaggca tgcgccaaaa
     3001 agaacacata gatgccatgg acattatggg ggtcaatacc tataatttcc tgattggccc
     3061 caaagtactc ggaggggtac t
//
LOCUS       DAOQMC010000263         3080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_17648_length_3080_cov_5.087273, whole genome shotgun sequence.
ACCESSION   DAOQMC010000263 DAOQMC010000000
VERSION     DAOQMC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17648_length_3080_cov_5.087273"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PKV31_09160"
     CDS             complement(<1..733)
                     /locus_tag="PKV31_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNB93066.1"
                     /translation="MRKNVLFYLMAFAITMIVGCAAISKNGFTVSGEIKDAANMAVSL
                     DRIGGNNQIVPVGKANIDGQGKFKITSEEALKPGVYRLQIGERIAPIIVDGKENDIKI
                     KGALASFDDLSFEITGAKGTSEFQSAIKKLLDKTLDDAGAKTAVESATNPYAAMQIAM
                     IYFKKPTDAPIHTAVLEKLKKLDPTSDFISGYQSMINQMANPQPQGGGPISIGQMAPD
                     ISLPNPDGKNIALSSLKGKIVLLDFW"
     gene            1182..1913
                     /locus_tag="PKV31_09165"
     CDS             1182..1913
                     /locus_tag="PKV31_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNB93067.1"
                     /translation="MTNYMRIEPGTAAECRTPLAPLRSDPSEMSQLETQILYGQACDI
                     LESGNGDWIRIKVDGYEGWADRKHFYPKKYTRKYFLTRLITPVETLYGTQLLPFGSML
                     TQEEEQQITESKESTTCTKKKPLDFAPLFENAPYLWGGKSILGIDCSGYMQLIFGACG
                     IHLPRNASQQESLGNPIEFGHRQSGDLAFFSNDKGKIIHVGIIDEEGIWHASGWVHHD
                     PITAEGIVHFETGMITHRLASIKRI"
     gene            2364..>3080
                     /locus_tag="PKV31_09170"
     CDS             2364..>3080
                     /locus_tag="PKV31_09170"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNB93068.1"
                     /translation="MESNNSIDNKFGENGITFDDVLLVPAHSEVLPREVDISTRLTEK
                     IRINVPVISAAMDTVTEKELAISIAQQGGIGIIHKNMTIEAQAAHVRSVKRSESGMIL
                     DPVTLHENATIGDALNLMEANHIGGIPIIDHDQRLKGILTNRDLRFETVLTRPVTEVM
                     TKDRLVTAPKGTDLDNARNILQTYKIEKLPVVDSEGRLAGLITYRDIMKLKDFPSASK
                     DTFGRLLVGAAVGVTGDTEER"
BASE COUNT          903 a          725 c          583 g          869 t
ORIGIN      
        1 cccaaaaatc aagtaataca atctttcctt tgagtgaaga aagagcaata ttcttaccat
       61 ccgggttggg taggctgata tccggtgcca tctggccgat actgattggc ccgccaccct
      121 gcggttgagg attggccatt tgattgatca tggattgata tccgctgata aaatcagacg
      181 tcggatccaa tttcttcaat ttttcaagta cagctgtatg aatcggagca tctgtaggct
      241 ttttgaaata aatcatcgct atctgcatgg ctgcatatgg attagttgca gactcaactg
      301 ctgtctttgc ccctgcatca tccaaagtct tgtccagcaa tttctttatt gcactctgaa
      361 attcagatgt accttttgct ccggtgattt caaaactaag atcatcaaag gatgccaatg
      421 cccctttgat cttgatatca ttctcttttc catcaacaat gataggagca atcctctctc
      481 caatctgtaa tctatataca cccggcttca aagcctcttc cgaagtaatt ttaaatttgc
      541 cctggccgtc aatattggct ttacccaccg gaacaatctg attattgcca ccaatacgat
      601 ccaatgaaac ggccatattt gctgcatcct tgatttcacc tgaaacggta aatccatttt
      661 tgcttattgc ggcacatcct acaatcatgg tgatcgcaaa ggccatcagg taaaataata
      721 cgtttttacg catctttgtt ggtttttgat tattgaatta agacaatccg ggaaaactcc
      781 ctccttgtat gctgaatttt gttttcaaac aacgaaatta aagctatttt gttttccaag
      841 cttatatttt cccggctaat tgctattaaa agatttgtta atatgtacaa atccagtttg
      901 ccgacaaacc tgcagtgtgt tcaggcaggt catcaaaaaa ataatatgac ctatctacaa
      961 ttgaaataac cattttgcaa tgtttcaaat ttacttgatt ccaagcctcc cctgatgcga
     1021 ttttatctca aatccttata ccctgatccg actttagcaa ttcctttcat tatccgtttt
     1081 gacgaatgtg caaatatcaa aaaatcaatt ttccatcaat ctgcaaccaa tgcctgacaa
     1141 tcatcccaaa ttgcataact ttgtcttgaa aaccatcccg gatgacaaat tacatgagaa
     1201 tcgaacccgg cacggctgcc gaatgtcgca cacccctggc tcccttgaga agtgaccctt
     1261 ccgagatgtc ccaattggag acccagatac tatatggtca ggcttgcgat atactggagt
     1321 ccggcaatgg cgactggatc agaatcaagg ttgacggata tgagggatgg gccgacagga
     1381 agcatttcta cccaaaaaaa tatacaagaa aatatttcct tacccgactc atcacgccgg
     1441 ttgaaacttt gtatggcacc cagcttcttc cctttggatc tatgctcaca caggaagaag
     1501 agcaacaaat taccgagtcc aaagagtcaa caacatgcac taaaaaaaaa ccgttagatt
     1561 tcgcacccct ttttgaaaat gctccttatc tatggggcgg caaatcgatt ctgggcatcg
     1621 attgcagtgg atacatgcaa cttattttcg gtgcatgcgg aattcatctg cccagaaatg
     1681 cctcacaaca ggaatctttg ggcaatccga ttgaatttgg ccatcggcaa tcaggcgatc
     1741 tcgcattttt ttccaacgac aagggcaaaa tcatccatgt tggcatcatc gacgaagaag
     1801 gtatctggca cgcaagcggc tgggttcatc acgaccccat tacagctgaa ggtatcgtcc
     1861 actttgagac gggcatgatc acacatagac tcgcatccat caaaaggatc taaatctcag
     1921 cctgctcaat agatataatt tttttaaccc ctgatttagt tagatttttc catctgtaac
     1981 atgactattt atttcagcat gtatctgtgc aatttacaat acaaaatatt acctgcaagg
     2041 tactgctcat tactgcatat atttttaata cattatttcc gacttgcgta agggtataag
     2101 aatcagtgtt ttaaatatcg gatattttga ataatgtcat ctgatcaaag agtcctttac
     2161 ggttataccc cggcaaagtg tttcgaccta tcactacatg cgttttacat ataagaataa
     2221 ttactactac ttcatgattc tattgatctt agaatacttt tgatcgcgaa gctaaagtaa
     2281 ttcaggaatc tcatcttcgc caatcaataa attttcttca aaaaaactcc cttcatttca
     2341 aatcgattca cctatctttg cacatggaaa gtaacaatag tatcgacaat aaattcggtg
     2401 aaaacggaat cacattcgac gatgtccttt tagtccctgc ccattcggaa gtcctcccca
     2461 gagaagtaga tatatccacc cggctgaccg aaaaaatccg tatcaatgta ccggtgatct
     2521 cagcagccat ggatacggta acagaaaagg aactggctat aagtattgca caacagggcg
     2581 gtatcggcat catccacaag aacatgacga tcgaggctca ggcagctcac gtgcgcagtg
     2641 tcaaacgttc cgaaagcggt atgatcctcg atcctgtcac cctgcatgaa aacgcaacca
     2701 tcggcgatgc tctcaatctc atggaagcca atcacatcgg tggtatcccc atcatcgacc
     2761 atgatcaaag actgaaggga attctgacca acagggatct tcgctttgag accgtgctga
     2821 cccgcccggt tacagaggtg atgaccaaag accgtttagt tactgctccc aaaggtacag
     2881 acctggacaa tgcccgaaat attctacaga catacaaaat cgaaaaactg ccggtggtgg
     2941 actctgaagg tcggctggca ggcttgatca cctatcgcga catcatgaag cttaaggact
     3001 tcccttcagc cagtaaagat acatttggcc ggcttctggt aggtgctgcc gtcggagtaa
     3061 ccggtgacac ggaagaaagg
//
LOCUS       DAOQMC010000264         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_18187_length_3030_cov_4.846723, whole genome shotgun sequence.
ACCESSION   DAOQMC010000264 DAOQMC010000000
VERSION     DAOQMC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18187_length_3030_cov_4.846723"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="PKV31_09175"
     CDS             complement(<1..1411)
                     /locus_tag="PKV31_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB93069.1"
                     /translation="MEDNGLLYIEDNIKTLDWKEHIRMRPGMYIGKLGDGQSNDDGIY
                     ILIKEIIDNAMDEYFMGHGKEIVIRISGDRVMVRDYGRGIPLGKVIDCVARINTGGKY
                     DSKAFKKSVGLNGVGTKAVNALSESFTVQSFRDGRTKKADFSKGELVKDYPEGKTDEK
                     NGTIIEFTPDNDIFRNFHFREDIVEPMLWNYAYLNSGLKINFNGKTFVSKNGLADMIA
                     GRTNPEELIYEPIHLTGDDIEIVLTHGHQYGEQYYSFVNGQNTTQGGTHLNAFREAVV
                     KVLRDFYKKDYDARDVRTSIIAAVSIRVEEPIFESQTKTKLGSTLMGPDQPSIRTYIM
                     DFLSKELDNFLHKNLEVAGQLQKKILQSERERKEIADIKKEVNKKAKKANIHNKKLRD
                     CRIHLNDKGGTPEQKENSMLFITEGDSASGSITKARDPFTQAVFSLRGKPLNCYGMTK
                     KIVYQNEEFNLLQHALNIEN"
     gene            1822..2529
                     /locus_tag="PKV31_09180"
     CDS             1822..2529
                     /locus_tag="PKV31_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93070.1"
                     /translation="MPEKIPCDVYQTMKVDQLLETPREVRDSVWRTEFFNAVTTACFK
                     CGEPQMSHGPDGFPYFELHLPEPEESFESYCIHNLMDYLLEIGYGVVLNPKGEDADWV
                     FSYGDIVNLSLNQEIYSVPDHDPLPEYYATDEEEKVMIFQPSEEYLPGIVKKMLHSFL
                     TYLHVKEPSMLAFIRSGGTENSGVTQLSFNIFPEDYGSEDEYLIRLKQLSWFLPRHYI
                     ITGVSRSNPITQYFEPL"
     gene            complement(2620..>3030)
                     /locus_tag="PKV31_09185"
     CDS             complement(2620..>3030)
                     /locus_tag="PKV31_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB93071.1"
                     /translation="RLDRDTTGLLLITNDGDLTKRLSHPSYEVKKIYHATLNKPLSEA
                     DLAKIRAGITLEDGFIKVDGISYVRDAGPHEVGITLHSGRNRIVRRIFEFFGYDVVKL
                     DRVYYAGLTKKDLPRGRMRFLTKEEVIRLKHFKV"
BASE COUNT          846 a          664 c          617 g          903 t
ORIGIN      
        1 cattttcaat attgagggca tgttgcaata aattaaattc ttcattctgg taaactattt
       61 tttttgtcat tccataacaa ttcaaaggtt taccacgtaa actgaacact gcctgagtaa
      121 agggatcccg cgccttggtt atggatccgc ttgctgaatc tccttctgtt ataaacagca
      181 tgctattttc cttctgttcc ggcgtaccac ccttatcatt gaggtggatg cggcaatccc
      241 gcaatttttt attgtggata ttggcctttt tagccttttt attgacttct tttttaatat
      301 cagcaatctc cttacgctct ctttcacttt gtagtatctt tttttgcagc tggccggcta
      361 cttcgaggtt cttgtgaagg aaattatcca gctccttaga caggaaatcc ataatatagg
      421 tgcgtatgga tggctgatca gggcccataa gtgtcgaacc aagctttgtc ttggtctgtg
      481 attcaaagat tggttcttcc acgcggatac taactgcggc gatgatggaa gttcgcacgt
      541 cacgtgcatc gtaatctttt ttataaaaat ctctaaggac cttcaccact gcttccctga
      601 atgcattgag atgagtacct ccctgtgttg tattctgacc gttgacgaaa gaataatatt
      661 gctcaccata ctgatgacca tgggtcaaaa cgatttcaat gtcatcaccg gtaagatgaa
      721 ttggttcgta aatcaattcc tccggattgg ttctcccggc aatcatgtcg gccagtccgt
      781 ttttggagac aaatgtcttt ccgttgaaat tgatctttag accactgttg agataggcat
      841 aattccacaa catgggttcg acaatgtcct ccctaaagtg aaaattcctg aaaatatcat
      901 tatcgggtgt aaactcgatg atagtgccgt ttttttcgtc ggtctttcct tcaggataat
      961 ccttaaccag ctcacctttt gaaaaatctg cctttttggt acgaccatct ctgaacgact
     1021 gcacggtaaa agactcgctt aatgcattga cggccttggt tccgacacca tttaatccaa
     1081 cggacttctt aaaagccttg gaatcatatt ttcctcctgt gttgattcgg gcgacacagt
     1141 ctatgacttt tcccaaaggt atacccctgc cataatcgcg gaccatcacc cgatcaccgg
     1201 atatacggat gacaatttcc ttaccatgcc ccatgaagta ttcatccatg gcattatcaa
     1261 taatctcctt aataaggata taaataccat catcattaga ctgtccgtca ccaagctttc
     1321 caatatacat acccgggcgc atacgtatat gctccttcca atcgagtgtc ttgatattgt
     1381 cctcaatgta gagtaaacca ttgtcttcca tgctgcaaaa ttacatatat ttttttataa
     1441 attaaataaa agccttatac aatgaggatt tggtgtaagt aagccgaatt gccggataga
     1501 cctcctttgg ccgatccaat tcttgaaaat aaaccagggt gaaaatttat gtacgagtgg
     1561 agctatggta gcaaaatgaa tccaagaata gaacaaatag cctgaagggc cgatgtttcc
     1621 cgggaatcga tggtgtatgg gagggagcgt aaggttaatc aaatatcgac agggcttagg
     1681 ataatgaggt gaaggaatgt ggtgaccatg ctatgacgaa aattcacgga aaaggagttc
     1741 tctgaataag tgaatttaag caggcaaatt gacagtaatc gcattcaatt ttcctatctt
     1801 cacgccacaa attctcatat tatgccggaa aaaatcccct gtgatgtata tcagaccatg
     1861 aaggtggatc aactgttgga gacgcccagg gaggttcgcg actcagtatg gaggactgaa
     1921 tttttcaatg ctgtgaccac ggcatgtttt aaatgtggag aaccacagat gtcacacggg
     1981 ccggatggct tcccttactt tgagttacat ttacctgaac cggaagagag ctttgaaagt
     2041 tactgtatcc acaatttgat ggactatctg ttggaaatcg gttatggcgt ggtccttaat
     2101 cccaaggggg aagatgccga ctgggtattt tcctatggag atattgtcaa tctgagccta
     2161 aatcaagaga tttattccgt accggaccac gatcctctac cggaatatta tgcaacagac
     2221 gaagaggaaa aagtaatgat attccagcca tcagaggaat atcttcccgg tatcgtaaaa
     2281 aaaatgttgc attccttcct gacctacctg catgtgaagg agccgtcaat gcttgcattt
     2341 atcagatccg gcgggactga aaattcaggg gtaacacaac tatctttcaa tatatttcct
     2401 gaggattatg gaagtgaaga tgaatacctc atccgactta aacaattgag ttggtttctt
     2461 ccacgacatt atataatcac gggtgtctcc aggagtaatc cgatcactca gtattttgaa
     2521 cctctataag agagtaccgt agccaaggtc actacacgga ttgatgcagg tagtaaacac
     2581 ctgactgact catgctttat acctatttca aacaagtaat taaaccttaa aatgcttcag
     2641 cctaatgact tcctcctttg tcaaaaaccg catccttcca cgaggaagat cctttttggt
     2701 caatcctgca taatagactc tatccagttt gactacatca taaccgaaga attcaaaaat
     2761 ccggcggacg attcggttgc ggcctgaatg caaggtaatt ccaacctcgt gggggccggc
     2821 atccctgaca tatgaaatgc catccacctt aataaagcca tcttccagtg ttattcccgc
     2881 cctaatcttt gcgagatcag cttcagacaa gggtttattg agtgtggcat gataaatctt
     2941 tttgacttcg taggaaggat gtgaaagcct tttagtcagg tccccatcat tggtaatcag
     3001 caacagcccg gttgtatccc tgtcgagcct
//
LOCUS       DAOQMC010000265         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_18253_length_3024_cov_5.048164, whole genome shotgun sequence.
ACCESSION   DAOQMC010000265 DAOQMC010000000
VERSION     DAOQMC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18253_length_3024_cov_5.048164"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="PKV31_09190"
     CDS             <1..1804
                     /locus_tag="PKV31_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB93072.1"
                     /translation="TDQPIANAGADATLDCISPFITHSGTFTSTQNNFISVTWSTNNG
                     SINGPINQLTTSFESAGFYVLTVTNSLNNCSDTDTMQVFNNNDKPIISIDPTDTLTCS
                     TTSITVVPHWSNSGISPIIEWITMDGNLVSASTDSIAVVDKAGTYQISVKNQQNGCIS
                     TLNITVIEDKKIPAGNITPPDTLDCQKTETILTFNNNDPAFLFLWSTTNGTLVGATDT
                     NEAVAGKEGTYTLQIIDNRNGCQLNLIQDVIANRVLPIANAGNDLSLNCFNPQISLQG
                     ETGNSGSLEITWSVLGSSGNIVNGEKTLTPLVDLAGTFIMTILNTENGCKNADTVEVI
                     NKITQPAFVNPTIIDANCLGKNGKISFENIASGDGPYQFWLNGNLVNPTNNQFTGLSS
                     GTYHIEGQDSNGCKFEFTGNIKNGENIAFDLPDTVTLNYGESFDIEPIFFFDTVGMSY
                     ENWIGGNFFSCNPCLYPTISPTQNSILTLTVADRDGCKATESMVVRVLRKKAHIFVPN
                     AFSPGDYNGINDKVTVYTDESVIKEIQEFRIFNRWGAEMFYRNNFAPNDEALGWDGYF
                     QGKMCNPNVFVYFARCRDIYGQEITVKGSITLTQ"
     gene            complement(2188..>3024)
                     /locus_tag="PKV31_09195"
     CDS             complement(2188..>3024)
                     /locus_tag="PKV31_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB93073.1"
                     /translation="IIPVGLGLPLEGDTLCIPNNVGPSSNFQLCVNMGGALGDISWLD
                     ANTPPIISFQTTTDPFAPYHDDVLIVPGPNLPVVQVQGSYEVQKSAHNLGINKAFADK
                     TFIDPLSQYVSTINDGFEGLYPIFRANPYDSSPWDYWDRATNPNDASGILTNPDMSFA
                     KASLFMDTIINYFAPRSCLVLNLGCDLTDYVSSTKTISAEVAGVSASPNPAGDFVIIK
                     SENKKIHGIVLYDGNGRTLTAIPNIMNKEYRLERNNIPPGVYYAQVRFEKESATVKFI
                     FN"
BASE COUNT         1001 a          574 c          589 g          860 t
ORIGIN      
        1 cacagatcaa ccgatagcaa atgcaggagc agatgctacg cttgattgta tctcaccatt
       61 catcactcat agtggcacct ttacctcaac tcagaataac tttatcagtg ttacatggag
      121 tactaataat ggaagtatta atggcccaat caatcaattg actacatcat tcgagtcagc
      181 cggattttat gttttaactg tgaccaatag cctgaataat tgttcagata ctgatacaat
      241 gcaggtcttt aacaacaacg acaaaccgat aatcagcatc gatccgactg acacccttac
      301 atgcagcact acatccatta cagttgtacc tcattggtcc aattcaggta tatcccctat
      361 cattgaatgg attactatgg atggaaatct ggtgagtgcc tctaccgatt cgattgctgt
      421 agttgataaa gcaggtacat accagatcag tgtaaaaaat cagcaaaatg gctgtatttc
      481 aacacttaac attactgtta ttgaggacaa gaaaatccct gccgggaata tcactccacc
      541 agacacattg gattgccaaa agactgaaac gattttgact tttaacaata atgatcctgc
      601 ctttctattt ttatggagta caactaatgg gaccctggtc ggcgctactg atactaatga
      661 agcagtagcg ggaaaggaag gcacatatac tttgcaaatc atcgacaatc gcaatggatg
      721 tcaattaaat cttatccaag acgtaattgc caacagagta ttgcctatcg caaatgccgg
      781 aaatgatttg agtttaaatt gctttaatcc acaaatatca cttcagggcg aaacaggtaa
      841 ttcaggcagt cttgaaatta cctggagtgt tttaggaagc agcggaaata tcgtaaatgg
      901 agaaaaaacg ttgacgccac ttgtagatct ggcaggaacg tttatcatga ctatattgaa
      961 tacagaaaat ggctgtaaaa atgccgacac tgttgaagtc ataaataaga ttacacaacc
     1021 ggcttttgtc aatccgacca taattgatgc caattgtttg ggaaagaatg gtaaaatttc
     1081 ctttgaaaat atagcttcgg gtgatggacc gtatcaattt tggttaaatg ggaaccttgt
     1141 aaatcctaca aataatcaat ttaccggatt gagctcgggt acataccaca ttgaggggca
     1201 agattcgaat ggatgcaaat ttgaatttac tggaaatata aaaaatggag aaaatatcgc
     1261 ttttgacctt cctgatacag tcactcttaa ttatggagaa tccttcgata tagaacccat
     1321 atttttcttc gacacagttg gcatgagcta cgaaaattgg atcggaggca acttcttcag
     1381 ttgtaatcca tgcctctacc ctactatatc tccaactcaa aattccatcc tgacgcttac
     1441 tgtcgccgac agagatggct gtaaagccac tgaatcaatg gtggttaggg ttttgagaaa
     1501 aaaagctcat atttttgtac caaatgcttt cagtccgggc gattacaatg gtatcaatga
     1561 caaggtgact gtatatactg atgaatccgt catcaaggaa attcaggaat tcagaatatt
     1621 caatcgttgg ggagccgaaa tgttttacag gaacaatttc gctcctaatg atgaagcttt
     1681 aggttgggat ggctattttc aaggtaaaat gtgcaatcca aatgtttttg tttattttgc
     1741 ccgttgcaga gatatttatg gacaggaaat aactgtcaaa ggaagtatta cgctcacaca
     1801 ataattgcaa ctaatccatc ttcatacatg ggaattgaag aacgagaatt aatttttaaa
     1861 gactaatcca caatcattca tatagatagc tgccaaattg aatgataagg tattagaaac
     1921 tattcaagga aaaacttagc attcataaat gcctaccggt aacaaataag gtacaccttt
     1981 ttccataaga gttctttata aggcgactat tcactttttc aaaatgataa ccttacctta
     2041 attcaaacag ggcaaggatg cttatttggg caatatgttt cccggataaa aaccatatcg
     2101 aagagtaggt atataagaca agaatgatta taaaaaaagc gaggcatctg cctcgcttca
     2161 ttatttgatt cttgaaaaga tccgggatta gttaaagata aatttaacgg tagccgattc
     2221 cttttcaaac ctgacctgtg cataataaac accgggagga atattgtttc tttccaatcg
     2281 atattcctta ttcatgatat tcggtattgc agtcaaagtt cttccattac catcatatag
     2341 gacgatacca tggattttct tgttttcaga tttaatgatt acaaaatcac cggcaggatt
     2401 tggagaagca gacacacctg ctacttcagc cgaaatggtt ttcgtagaag atacataatc
     2461 tgtcaagtca catcccagat ttagcaccag acaagaacgg ggtgcaaagt agttgatgat
     2521 ggtatccata aacaaggaag ccttagcaaa tgacatatca ggattagtca gaataccact
     2581 tgcatcattg gggttggtgg ctctatccca gtaatcccat ggagaggaat cgtatggatt
     2641 agccctgaat atagggtaca aaccttcaaa tccatcattg atggtagaaa catactgaga
     2701 taatggatca ataaaggtct tatcagcaaa tgccttgttg attcccaaat tatgggcact
     2761 cttttggact tcataagaac cttgtacctg aactacggga agattcggtc ccggaacgat
     2821 aagtacatca tcatgatatg gagcaaaagg atctgttgtt gtctggaagg aaatgattgg
     2881 tggtgtgtta gcgtctaacc aagaaatatc accaagggca cctcccatat ttacacaaag
     2941 ctggaaatta gaagatggtc caacattatt aggaatacaa agggtatctc cttccaatgg
     3001 aagacccaaa cctacgggaa taat
//
LOCUS       DAOQMC010000266         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_18281_length_3022_cov_4.014493, whole genome shotgun sequence.
ACCESSION   DAOQMC010000266 DAOQMC010000000
VERSION     DAOQMC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18281_length_3022_cov_4.014493"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKV31_09200"
     CDS             complement(<1..391)
                     /locus_tag="PKV31_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNB93074.1"
                     /translation="MGILQAIIIAIVEGLTEYLPISSTAHMGFVAALMGMEESEYLKM
                     FQISIQFGAILAVVVLYWKKFFDFSNLTFYTKLIIAVIPALVLGFLFDDQIEAVLGNQ
                     IAISSVLILGGVVLIFIDRWFTNPKYTD"
     gene            complement(438..785)
                     /locus_tag="PKV31_09205"
     CDS             complement(438..785)
                     /locus_tag="PKV31_09205"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNB93075.1"
                     /translation="MSQKKKSQASSGQPLKPTASTINQESTERKEATIKQVDKGNLVF
                     GKDNFKWMFIGMGVMLLGYILMSGGTMPNPETWDPSLIYSFRRITLAPFIILLGLAIE
                     VYAIFYIPKKKTS"
     gene            complement(832..1713)
                     /locus_tag="PKV31_09210"
     CDS             complement(832..1713)
                     /locus_tag="PKV31_09210"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNB93076.1"
                     /translation="MARKNRIGIPKPKPNYFLSIVSIALVLFLLGLMALLSLNTNKLI
                     NSFKDNVNLIAELRDSVPSGKIDTLKEFISSLEAVKASTVDIVTKEEALSIMKKEMGD
                     DFLLDEMTNPLNDAIVFNVKADYLDSLQLAGIKTTILNAYDFVTNVYYQETFVGKVTE
                     TLSKIGIGILVISILFLLIALTVMHGTLKLSMYSNRFLIKNMQLVGASKGFIQRPFLW
                     KGVWSGLLSALVACLMLSGVIWAAYLYIPDVSGIIDLNEVGILFGAILILGILITVIS
                     TFFIINRYIRLRREDMF"
     gene            complement(1940..>3022)
                     /locus_tag="PKV31_09215"
     CDS             complement(1940..>3022)
                     /locus_tag="PKV31_09215"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNB93077.1"
                     /translation="DATDDMVTIEAANAIKQYGVGIKCATITPDEARVKEFNLKQMWK
                     SPNGTIRNILDGTVFREPIVMSNIPRLVPNWTAPICIGRHAFGDQYKATDFVTKGKGK
                     LTVKFEGEDGSVQEFEVYNFKGDGVALTMYNTDESIAGFARACFNMSLNKGWPLYLST
                     KNTILKKYDGRFKDIFQEIFDNEFKARFDELGITYEHRLIDDMVASALKWHGNFVWAC
                     KNYDGDVQSDTVAQGFGSLGLMTSVLVTPDGKTMEAEAAHGTVTRHYREHQKGNPTST
                     NPIASIYAWTRGLAFRGKLDDNQALIHFAETLEAVCVETVESGKMTKDLAVCIHGNKV
                     NHGEHYLYTEEFLDALDQNLKAKLGQ"
BASE COUNT          889 a          636 c          561 g          936 t
ORIGIN      
        1 catctgtgta ttttggattg gtaaaccaac gatctatgaa gatcaaaacg acaccgccca
       61 gaataagtac tgaggaaata gctatttgat taccgagaac cgcctctatt tggtcatcaa
      121 aaagaaaacc cagcactaaa gcaggaataa ccgcgattat taatttggtg taaaaagtta
      181 ggttactgaa gtcgaagaat tttttccaat atagaacaac gacagctagt atcgctccaa
      241 attgaatcga aatttggaac atctttaaat attctgactc ttccataccc atcaatgcag
      301 ctacaaaacc catgtgcgca gtggaagaaa ttgggagata ctccgtcagg ccttcgacta
      361 tagcgatgat gattgcctgt agaatgccca tatggttgtc agtgtataaa gattaaatat
      421 tgggacaatg aaggacgtta ggatgttttt ttcttaggaa tgtaaaaaat ggcatagact
      481 tctatggcaa gacctaacag tatgataaac ggcgctaatg taatcctcct gaaactgtat
      541 ataagggaag gatcccatgt ttcagggttg ggcatagttc ccccggacat caaaatatac
      601 cctaacaaca tcacacccat gcctataaac atccatttga aattatcctt tccgaatacc
      661 aaattgcctt tgtcaacttg tttaatagtg gcttccttcc tctctgtgga ctcttgattt
      721 atagtggatg cagtaggctt taatggttga cctgaagatg cctgactttt tttcttttgg
      781 ctcatatatt caaaaattaa tggttcaaag gtagaatgta ttgcccaatt atcaaaacat
      841 gtcttccctt cttagacgaa tataacgatt gattatgaaa aatgtggata taactgtgat
      901 taagatgccg agtatcaata tcgccccaaa caggatgcca acttcattca aatcaatgat
      961 tccactaaca tccgggatat acaaataggc tgcccagatc accccgctaa gcattaaaca
     1021 ggcaacaagg gcactcaaca agccactcca tacacctttc cacagaaatg gtcgctggat
     1081 aaatccttta ctcgcaccca ccaattgcat attttttatt aaaaatcggt tggaatacat
     1141 cgacaacttc aatgtcccat gcataacagt caacgcaatt aaaaggaaca gtatcgatat
     1201 aaccagaatg ccgattccga tcttgcttaa tgtttccgtg actttgccaa caaaggtttc
     1261 ctggtaatag acattggtta caaagtcata cgcattaagg atggtggttt tgatccctgc
     1321 aagttggagt gaatccagat aatctgcctt gacattgaat actattgcat cgttaagagg
     1381 attggtcatt tcgtcaagaa ggaaatcatc acccatttcc tttttcatga tggaaagagc
     1441 ttcctcttta gtaacaatat ctacggtgga tgccttaact gcttccaggg atgagatgaa
     1501 ttccttcagt gtgtcaattt ttcctgaagg taccgaatcc ctaagctccg caatcagatt
     1561 gacattgtcc ttgaatgaat tgattagttt gttggtattc aacgacagta atgccatcaa
     1621 tcccaacaga aaaagaacaa gcgctatact cactatggac aagaaatagt taggtttggg
     1681 cttcgggatg ccgattctat tctttctggc cataagtttt tacggtttta tggttcaatc
     1741 tgtatgattt tacatatata atgtgcaaat atataatttc tttataatat ataattttaa
     1801 attttatttt ttacctgaag taagttattg aatctcttca attcgtatcc taatggatta
     1861 tattttcttg ttacaggaat caacaaaaaa gccgttgtaa aatatcacaa cggcttttgt
     1921 tttactaatt atttgatgat tattggccca gtttagcctt taggttctga tccaatgcat
     1981 ccaggaattc ttcagtatac agataatgtt cgccatgatt gaccttgttg ccatgtatac
     2041 acactgcaag gtctttggtc atctttccgc tttcaacagt ttcaacacaa actgcttcca
     2101 gtgtttctgc gaaatgtatt aaagcctgat tatcatccag tttgccacga aatgcaaggc
     2161 ctcgtgtcca ggcgtagatg gaggcaattg gattggttga tgtagggttg cctttctgat
     2221 gctctctgta atgacgggtt acagtcccat gagctgcttc tgcttccatg gtctttccgt
     2281 ccggggttac aagtactgat gtcataagac caagtgagcc aaatccctgc gctactgtgt
     2341 cactctgtac gtccccatcg taattcttac atgcccatac gaagttgcca tgccatttta
     2401 aggcagatgc caccatgtca tcaatcaaac gatgctcata ggtgatgccc aattcgtcaa
     2461 agcgagcttt gaattcattg tcaaagattt cctgaaaaat atccttgaat ctgccatcgt
     2521 atttcttcag gattgtattt ttggtagaca aatacaacgg ccaaccttta ttcaacgaca
     2581 tattgaaaca ggcacgcgca aaacctgcaa tactttcatc tgtattatac atggttagtg
     2641 caacgccatc tcctttgaaa ttataaactt caaattcttg tacactacca tcttcgccct
     2701 cgaattttac ggtcaatttg ccttttcctt tggttacaaa gtctgtcgcc ttatattgat
     2761 caccaaaggc atgacgaccg atacaaatcg gtgcagtcca gttagggaca agacgcggaa
     2821 tgttactcat gacaatcggc tcccggaata cggtaccgtc caggatattt cggatagtac
     2881 cattgggtga tttccacatt tgtttaaggt taaattcctt tacccgtgct tcatccggag
     2941 taattgtcgc acatttgata cctacgccat attgcttgat ggcattggca gcttcaattg
     3001 tcaccatatc gtctgtggca tc
//
LOCUS       DAOQMC010000267         3006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_18459_length_3006_cov_6.013894, whole genome shotgun sequence.
ACCESSION   DAOQMC010000267 DAOQMC010000000
VERSION     DAOQMC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18459_length_3006_cov_6.013894"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="PKV31_09220"
     CDS             complement(<1..1789)
                     /locus_tag="PKV31_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNB93078.1"
                     /translation="MRRIKNIAVLGSGVMGSGIACHLANAGFEVLMLDMSSDGADRNQ
                     IANTALNRALKSSPAPLYSKKFRNRIHTGNFDDDLSRIKNVDWVIEVVKEDLNIKRLI
                     FNKIDAVRKPGTIITSNTSGIPVSLMVEGMSDDFKKHFCITHFFNPPRYLALLEIIPG
                     TDTDPEIIEFLMVFGNKILGKRTVLGKDTPAFIANRIGVFSMGKAFQLTKELGLTIQS
                     VDKLTGVAIGRPKTGTFRLGDLVGLDTAMAVLNGIRRNCPDDALAGDGEIPEFFARLI
                     ENNWLGDKTGRGFYQKTGEKDAKGKPVILGYNLNTFEYEADPGQKLESLDTSKQIDDP
                     EKRIRVLFDYKDAGGLFVRKSLAYTFIYAALRIPEIADNLYSIDDAMRAGFAWDYGPF
                     QYWDIVGLDKGIEAIRELGLEVPEWVVLMKESGNTTFYRNDAGIISFYDPNSRSYKAL
                     PGAEKSIQLNLLADQKVVYKNDEIRLWDIGDDVLCLEFTSKMNAIGGGILNGIQDAIT
                     LAEEGNWRGIVIGNNSKNFTVGANLMMIAMLAYEQEFDQLNMAVDLFQKTTMRCRYSK
                     IPVVAATQGYVFGGGCEMVMHCDATAVAAE"
     gene            1909..2814
                     /locus_tag="PKV31_09225"
     CDS             1909..2814
                     /locus_tag="PKV31_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93079.1"
                     /translation="MATNQEKREVVNSTKGFSSSLFLHSMLILIGISPLATFNAPIWE
                     EEQPGMEVALGFPDEGQGNDEPTPGNNEPGGLPPSSSNTTASQTSSPLPTTTSPTPTS
                     SKDEAMVTNDNAEAVDVAAANEAKRRKAEQEQIEAEQRRKAELLAEANRKAEAEKKAR
                     EQAEYEASKGKYGGLFKGSGTGRGNAGKPGNQGDPKGDPNAKNLEGIHTGGKGRIGGG
                     LGNRGVLSEPKIVDNSSKEGRVIIKVCVNSEGTVTSAEFTLKGSSTQDATLVNIARRN
                     ALKFKFTPSDVSQQCGTITYDFRLE"
BASE COUNT          937 a          674 c          585 g          810 t
ORIGIN      
        1 attcagcagc cacggcagtt gcatcgcagt gcatcaccat ttcacatcct ccaccgaaaa
       61 cgtatccttg tgttgcagct acaaccggta ttttactata ccgacagcgc atagtcgttt
      121 tttggaaaag atcgactgcc atattaagct ggtcgaattc ctgttcataa gccaacattg
      181 ctatcatcat cagattagcc ccaactgtaa agttcttgct attgttgcca ataactattc
      241 cacgccaatt accttcttca gcaagggtaa tggcatcctg gattccattg agtattccgc
      301 caccaatagc attcatcttg gaagtgaatt ccagacacaa aacatcatca ccgatatccc
      361 aaagacggat ttcatcattt ttatatacta ccttttgatc agcgagaaga ttcagctgaa
      421 tagatttttc agcacccggt aaagccttgt agctcctaga atttgggtca tagaatgaaa
      481 ttattcctgc gtcattcctg tagaaagtgg tattgccaga ttctttcatc aaaacaaccc
      541 actccggaac ttcaaggccc aattccctga ttgcttcgat acctttatcc agtcccacaa
      601 tatcccaata ctggaatggg ccgtaatccc aggcaaatcc tgcgcgcatg gcatcatcta
      661 tggaatacag gttatctgct atttcaggaa tcctcaaagc agcataaatg aaagtataag
      721 ccaaggattt cctaacaaat aaaccaccgg catccttata gtcaaaaagc actctaatgc
      781 gcttctccgg gtcatcgatt tgtttggaag tgtcgaggct ctcaagtttt tggccgggat
      841 ctgcttcata ctcaaaggta tttaagttat agcccagaat aacaggcttc cctttggcat
      901 ccttttcgcc tgtcttctga taaaatcctc tacccgtctt atctcccaac caattattct
      961 ctatgagacg cgcaaagaat tccggaattt ctccatcacc ggcaagggca tcatccggac
     1021 aattgcgccg tataccattc agaacagcca ttgctgtgtc aagacctacg aggtcaccaa
     1081 gtctgaaagt acctgtttta ggccttccaa ttgccactcc tgtcaactta tcaacggact
     1141 gaatagtcaa tccaagttct ttggtcaact gaaatgcttt tcccatagaa aatacgccga
     1201 ttctgttggc tataaatgcc ggtgtatcct tacccaaaac ggtgcgcttg ccaagaattt
     1261 tattcccaaa aaccatcagg aactcaataa tttcgggatc tgtatctgta cccggaataa
     1321 tttcaagtaa ggcgaggtac cttggagggt taaaaaagtg agtaatgcaa aaatgttttt
     1381 taaaatcatc actcattcct tcaaccatca aacttaccgg aatacctgag gtgttggatg
     1441 ttataattgt acctggtttt ctgacagcat caatcttatt aaagatcaag cgtttgatat
     1501 tcagatcttc ctttactact tcaatgaccc aatcaacatt ctttattcgt gacaagtcat
     1561 catcaaagtt gcccgtgtga atacgattcc tgaatttctt agaatacagg ggggcaggtg
     1621 atgatttcag tgccctattt aaggcagtat ttgcaatttg atttctatca gcgccatcac
     1681 tagacatatc tagcataagc acctcaaaac cggcatttgc caagtgacat gcaatacctg
     1741 accccatgac cccagagccc aaaactgcaa tgtttttaat tcttctcata tacttttatt
     1801 gaaaatatta atattataca ttaaaaataa aaacaatatg tatctttgtt tttgaaacct
     1861 caataataag aaaaatttca atttaaacgt ttatatagaa aagttgttat ggcaaccaat
     1921 caggaaaaaa gagaagtggt caacagtacc aagggattca gttcaagtct ttttttacac
     1981 agtatgctga tcttgatagg tatctcccca ttggctacct tcaatgctcc tatctgggaa
     2041 gaagaacaac cgggaatgga ggttgcacta ggttttcccg atgagggtca aggcaacgat
     2101 gaacctacac ccggaaacaa tgaaccggga ggactcccac cttcttcaag caatactaca
     2161 gcctctcaaa ccagctctcc attgcctact acaacatcac ctacgccaac atcgagcaaa
     2221 gatgaagcta tggtcacaaa tgataatgca gaagcagtgg atgtggcggc agcgaatgaa
     2281 gcaaaaagac gaaaagcaga acaagaacag attgaggcag agcaacgaag aaaggccgaa
     2341 ttattagccg aagccaaccg taaagcagag gccgaaaaaa aggcgaggga gcaagcagag
     2401 tatgaggcat ctaagggtaa atacgggggc ttatttaaag gttccggaac aggaagagga
     2461 aacgcaggaa agccaggaaa tcaaggcgat cccaagggtg atccaaatgc caagaatttg
     2521 gaaggtatcc ataccggcgg aaaaggaagg attggggggg gattaggcaa ccgaggtgtg
     2581 ctttctgaac caaaaatagt cgataatagc tcaaaggaag gcagagtcat catcaaggtt
     2641 tgtgtaaata gcgaagggac ggttacaagt gcagaattta cattaaaagg ctcttctact
     2701 caggatgcaa ctcttgtaaa cattgcgcgg agaaacgccc tgaagtttaa atttactcca
     2761 tctgatgtat cgcaacaatg cggaacaatt acatatgatt tcagacttga ataatcaaaa
     2821 agagatcact aatttccaag taccttatta gtttactatg aaaaggtttg attagctcat
     2881 atcctgttgc catattaaga tcaacctaat ttcaatttct tagcaatcgg cctatgcctg
     2941 accattctgg cgatgatgtc atccaggatt tcacctttca tgcggtgcct gttgtagcgg
     3001 tagcaa
//
LOCUS       DAOQMC010000268         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_18496_length_3003_cov_3.627883, whole genome shotgun sequence.
ACCESSION   DAOQMC010000268 DAOQMC010000000
VERSION     DAOQMC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18496_length_3003_cov_3.627883"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2978)
                     /locus_tag="PKV31_09230"
     CDS             complement(<1..2978)
                     /locus_tag="PKV31_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNB93080.1"
                     /translation="MNSRNESLGKPWSKVVNFLDTKPAVRFAGKGNIMPESDGLILPF
                     EAINLNAVDVEIVKIFSDNVLQYYQNYNGYEDSYEINRVGRIILQQKIQLSSLNSAIN
                     KSSWVRYGLDLNKLITTDRNSIYQVRIGFRRAYTSYLCKSGGDWKERELSTEPVAYGE
                     EPVSIYDHNYDGFAEEYDEDYNWLNRENPCKPMYYNKDNFIAQSVYKSNIGLIVKGND
                     NGNFFVAATNLLTAKPLSGVEIKFYDFQQQVIDKGLSNGEGFLNITLKSRPFMAIAEK
                     DGDKSYLLLDDGRSLSLSKFDVGGDSYQKGLKGYLYAERGIWRPGDSIFLNCIIEDKL
                     KTLPASHPIQLEWRDPRNTIIARKTYNYTSGSILPIHLATSPDAPTGVWAATVKVGGA
                     TFTKLMSIETVKPNRLKIKFDVDKKQLMAGQPLVNASLNAAWLIGTVAGNLRAVVDAN
                     IRVQSTPFTKYSDFIFSNSSSGKTFEKKVFEGNTDASGNASLKMNINLNEEIHQISNI
                     VFKTTVFEQGGNFSVDYTSETYSPFKVYVGVSVPHNQWGEPSIPDKKNAPISAIVVDQ
                     NGNPLSGINATINVYKVDWRWWWESDYSDANYYTGDFNEVPLAHYTGKTKGDGKIMFD
                     FRPPAWGRYLIKVTSDASPHVAGTFIYSGYPDYADAEQMSSIATSLPLGIDSKEVKVG
                     ETINLSFTAAAGSKALVTIEGGSSIVEHKWFDCKEGVNEVSIKTTDAMGSNAYAFVTL
                     MQPHKHPENDMPLRMYGVLPFKIVNPALVLNPVISMPQEIQPDKKAEISVSEQNGNEM
                     YYTIAVVDEGLLDITKFKTPNPYNFFYAKSALAVRTWDLFDYVLGAYSGKLNGIFAVG
                     GDMAAAQIEGAPKANRFVPAVVNLGPFKLKKGEKARHHFTIKNYIGSMRAMVVACNDK
                     AFGAAQQSVKVVKPLMVTTTLPRVLSPGETFNLPVNVFVTKNNIRNVTVKINDKNGYM
                     SVTGSSSKSLTFESSGEKMV"
BASE COUNT          851 a          692 c          538 g          922 t
ORIGIN      
        1 tataccattt tctcaccact cgactcgaat gtaagtgact tacttgacga accggtcaca
       61 gacatgtaac catttttgtc atttattttt accgtgacat ttctgatgtt attcttggta
      121 acaaatacat ttaccggcaa gttgaaagtc tcaccagggc tcaatacacg gggcaaggta
      181 gttgtaacca taagtggttt gaccaccttc acggattgct gtgcagctcc gaaagcctta
      241 tcattacatg ccactaccat ggcacgcatt gatcctatgt aattcttgat tgtaaagtga
      301 tgccttgcct tctccccttt tttcaactta aaaggaccga gatttacaac ggcaggaaca
      361 aaacgattgg ccttgggagc tccttctatc tgtgctgcag ccatatctcc tcctacagcg
      421 aagataccat tcaatttgcc actgtaagct cccaaaacat aatcaaacaa gtcccaggtc
      481 cttacagcaa gggcagactt ggcataaaag aaattatagg gattcggagt cttgaattta
      541 gtgatatcca gtagtccttc atctacaaca gcaatggtat agtacatttc attaccattt
      601 tgctcactca cggatatttc tgccttctta tccggctgaa tctcttgtgg catactaata
      661 acaggattga gtaccagtgc cggattgaca attttaaacg gaagtactcc atacattctc
      721 aaaggcatat cattttccgg atgtttatga ggctgcatta atgtcacaaa ggcgtaagca
      781 ttagacccca tggcatcagt tgtctttatc gaaacttcgt tgactccctc cttacaatca
      841 aaccatttat gttccactat ggaacttcct ccttcaatag tcacaagagc cttgcttcca
      901 gctgctgcag taaagcttag attaattgtc tctccgactt taacttcctt ggaatcgatt
      961 cccaaaggta atgaagtcgc aatacttgac atttgttccg catccgcata atccggatat
     1021 cctgaataaa tgaatgttcc ggctacatgc ggactggcat ctgatgttac tttaattaaa
     1081 taccttcccc aggcaggagg gcggaaatca aacataattt ttccatcccc cttggttttg
     1141 cctgtataat gagctaacgg cacctcgttg aaatcaccgg tataataatt agcgtcgctg
     1201 taatcactct cccaccacca tctccaatcg actttatata cattaatagt agcattgatt
     1261 ccggaaagag gatttccatt ttgatcgacc acaatcgcac taatcggagc atttttctta
     1321 tccggaatcg aaggttctcc ccattgattg tgtggcacag aaactccgac atataccttg
     1381 aatggagagt aggtttcaga tgtgtaatct acactgaaat tgcctccctg ctcaaaaacg
     1441 gttgtcttaa aaacgatatt actaatctgg tgtatttcct cgttgagatt gatgttcatt
     1501 tttaaggaag cattacctga agcatctgta tttccctcaa atactttttt ctcaaacgtc
     1561 ttcccagatg aggaatttga aaaaataaaa tcagaatatt tggtaaaagg agtactctga
     1621 actctgatat tggcatccac gactgcccta aggtttccag ccactgtgcc gatgagccag
     1681 gcagcattca gactggcatt gaccagtggc tgccctgcca tcaactgttt tttgtcgaca
     1741 tcaaatttta tttttagtcg attgggcttg acagtctcaa tactcatgag cttagtaaaa
     1801 gttgctcctc caaccttaac ggtagctgcc caaactccgg taggagcatc cggagatgtc
     1861 gccaaatgaa ttggcagaat agagcctgaa gtataattgt atgtcttacg agcaataatc
     1921 gtatttcgtg gatcccgcca ttccaattgg atcgggtgtg aggcaggcaa tgtctttaat
     1981 ttatcttcta tgatacaatt gagaaaaatt gaatctccgg gacgccatat gccccgttct
     2041 gcataaagat atcccttcaa ccctttttgg taagaatcac caccaacgtc aaacttgctt
     2101 aaagaaaggg agcgaccatc atccaacaat aggtaacttt tatcgccatc cttttcagca
     2161 attgccatga atggtcttga tttaagggta atgttcagga aaccctctcc attactcaaa
     2221 cctttatcga tcacctgttg ctgaaaatca taaaacttta tctccacccc ggatagtggc
     2281 tttgctgtaa gcaaattagt tgctgcaaca aaaaaattgc cgttgtcatt tcctttgacg
     2341 ataaggccaa tatttgattt atatacactt tgagcgataa aattatcttt attatagtac
     2401 atcggtttac agggattttc cctattcagc caattataat cctcatcgta ttcttcagca
     2461 aatccatcgt aattatggtc atatatacta acaggctctt ctccgtaagc aaccggctct
     2521 gtacttaact cgcgttcctt ccaatcccct cctgatttac ataaataaga agtatatgct
     2581 ctgcggaatc caatcctgac ttgatagata gaattgcgat ctgtagttat caatttattc
     2641 agatccaaac catacctgac ccacgatgac ttatttatgg ctgaattaag gctggaaagc
     2701 tggattttct gttgcaggat tatcctgcca acccggttga tttcatagct atcttcatat
     2761 ccattatagt tttgataata ttgaagtaca ttatctgaaa atatcttgac gatttctaca
     2821 tccactgcat taagattgat tgcttcaaaa ggcaaaatca gaccatcgct ctccggcatt
     2881 atgttccctt tgccggcaaa tctaactgca ggtttggtgt cgagaaaatt aactacctta
     2941 ctccagggct tgcccaatga ttcattcctt gaattcaaaa ccgaaggtgc aacacggaca
     3001 gtc
//
LOCUS       DAOQMC010000269         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_18540_length_2998_cov_4.209990, whole genome shotgun sequence.
ACCESSION   DAOQMC010000269 DAOQMC010000000
VERSION     DAOQMC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18540_length_2998_cov_4.209990"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PKV31_09235"
     CDS             complement(<1..1083)
                     /locus_tag="PKV31_09235"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNB93081.1"
                     /translation="MKEFLMKIKSFLIFALIIGVVSLFALYPRPIGDPIEKEVVILKS
                     VMNILDKAHFKPKDINDEFSEKVYKSYLESMDGGKRFFIQSDINQLKPYATLIDDEIK
                     GSKLDFFEKSLNLITMAQKRVREYYKEILSKPFSFDKKETISYDYEKLPYAKSEKELK
                     ARWEETLRAAVLDEVIQVMNRQENDSLKVNPEEKKSFAVIEKEAREKVGKDYDRLFDR
                     LEKLRREDRFGDFMNSITEQFDPHTSYFSPSDKEDFDIGMSGKLEGIGARLQADGEFT
                     KVSEVVVGGPAWKEKNLEAGDLIMKVAQDGQPAVDIRGMRLDDVVKKIRGKKGTKVIL
                     TVKKVNGSVQDITIVRDEVLMDESFAK"
     gene            1604..2509
                     /gene="folP"
                     /locus_tag="PKV31_09240"
     CDS             1604..2509
                     /gene="folP"
                     /locus_tag="PKV31_09240"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB93082.1"
                     /translation="MPDYSDNIETKTHGIQHVMPVLQTLNCRGSILDISKPLVMGIIN
                     ITDDSFYSGSRTPGPEGVVKKAKEMIHAGASIIDLGAMSTRPGSDEIPAGLEIERIIP
                     AVEAILSVVPEAILSIDTYRSKVAEKAIQAGAAIINDISSGDIDPDLPFLALQYKCPY
                     IAMHMQGRPKTMQNNPVYSDVVTEVLDYFIQKLEHFTQIGLVDVIFDPGFGFGKTLEH
                     NYTLLNNMEVFRITGKPILAGLSRKGMLWRPLNSDPSKVLPATIAVNLLALQHGATFL
                     RVHDVPENVQLLETFQIIKQFENKY"
     gene            2525..>2998
                     /locus_tag="PKV31_09245"
     CDS             2525..>2998
                     /locus_tag="PKV31_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93083.1"
                     /translation="MEENNNKIKATKDKRSLPGLISHFLWSLTKYSFIFLILMMMGVY
                     FLIQLPSFQNYAATKATEFLSRELKSEVTVGRIRINFFQKVLLEDFVVKDRQQDTLLN
                     IGELKAELKGGFLGLLDGKINITSAFIRNLDAHLVENCPKYDNNYQFLLNYLENGH"
BASE COUNT          922 a          653 c          528 g          895 t
ORIGIN      
        1 ctttgcaaaa gattcatcca tcaatacctc atccctgaca atggttatat cctgaacact
       61 gccattgacc tttttgactg taaggataac cttggtgccc tttttacccc tgatcttttt
      121 tacaacatcg tccaaacgca tgcctctaat atccactgca ggctgcccgt cttgtgcaac
      181 cttcataatc aaatcccctg cttcaagatt cttctctttc catgcaggtc ccccgacaac
      241 aacttcagag acttttgtaa attcaccatc cgcttgaaga cgggcaccaa taccttccaa
      301 cttgccggac attccgatat caaaatcctc cttgtcactc ggactgaaat agctggtatg
      361 aggatcaaat tgttccgtaa tgctattcat aaaatcgcca aaacgatcct cccgtcttaa
      421 cttttccaac cgatcaaaca accgatcata atccttgcct actttctccc tggcttcttt
      481 ttctatcact gcgaaggact ttttttcctc tggattgact ttcaagctgt cattttcctg
      541 cctgttcata acctgaatga cctcatccag aactgctgcc ctaagtgtct cttcccacct
      601 ggccttcaat tccttttcac tcttagcata aggaagtttt tcgtaatcat aagagatggt
      661 ttcctttttg tcaaaagaaa atggcttaga caatatttcc ttgtaatatt ccctgaccct
      721 tttctgagcc atagttatca aattcaggct cttctcgaag aagtcaagct tacttccttt
      781 aatctcatca tcgattaagg tagcataggg cttcaattga ttgatatcac tttgaataaa
      841 aaatctcttt ccaccatcca tactctcaag atagctttta tataccttct ctgagaattc
      901 atcgttgata tcttttggtt tgaaatgggc tttgtctagg atgttcatta ccgatttcag
      961 gataacaact tccttttcta tcggatcgcc aatcggtcgg ggatataagg caaataagct
     1021 tacaacccct ataatgaggg caaaaatcaa aaaagacttt attttcataa gaaattcttt
     1081 caatcgttat aattttctaa gcacaatagt aaacgaaaat cttaacatta ttatccctta
     1141 atagttggat aattgcaatt tttaagacag gattaactaa tttttagaca aaaatagaaa
     1201 atttcattgg atggcattcc tctaaagtac aatagtgtgt ataacaatat ggacttttaa
     1261 gatcaagaaa atatagatta aacaggaaat aatatgtttt tttcattcaa ttattaaaaa
     1321 atagaaatca tcaaacagtc aaagccaggc ctcaacagac agaccaagtc tgttagcggg
     1381 aacacatata agaaaactcg ttaaaaaacc tcattgcgtt ttcctacctt tgtatattaa
     1441 tccatggaca tggccattaa acttgctata tacagcacaa attcaaacca gtatggttcc
     1501 ggatttaata aagtcaaatt cattttctta accaggaatc tgatgtatct cgtgaatttt
     1561 gtcttgaaac accatactcc atcgctcacc tacccaaatg attatgccgg attattctga
     1621 caacatagaa acaaaaacac atgggattca acatgttatg ccggttctgc agaccttgaa
     1681 ttgccggggt tcaatcctgg acatcagtaa acctttggtc atgggtatca tcaatattac
     1741 tgatgattct ttctattccg ggagcaggac acccggcccg gagggagttg tcaaaaaagc
     1801 taaagaaatg atacatgcag gggcatccat cattgatctt ggtgcaatgt ccaccagacc
     1861 cggctcagac gaaattcctg ccgggctgga aatagaaagg ataatccctg cagttgaagc
     1921 aattctttcc gtagtacctg aagccatcct ttcaattgat acctaccgat cgaaagttgc
     1981 ggaaaaggct attcaggccg gagcggcaat catcaatgat atttcatccg gtgatatcga
     2041 tcccgacttg ccatttctcg ccttacaata taaatgtccc tacatcgcaa tgcatatgca
     2101 gggaaggcct aaaaccatgc aaaataaccc ggtctacagt gatgtagtga cagaagtatt
     2161 ggattatttc atacaaaagc tggaacattt cacacagatc ggcttggtag acgttatttt
     2221 tgatcccgga ttcgggtttg gaaagacatt ggaacataat tatacgttat tgaataatat
     2281 ggaggttttt cgaattacag gcaagccaat actggcagga ctgtcccgta agggaatgtt
     2341 gtggcgacca ttgaattccg acccttcaaa ggtattgcca gctactattg cggtaaatct
     2401 gttagctttg caacatggtg ctacattcct gagggtacat gacgtgccag aaaatgtaca
     2461 gttgttggaa acgttccaga tcataaaaca atttgaaaat aagtactgat accggggaaa
     2521 ataggtggaa gagaataata acaaaataaa ggcgaccaag gacaaaaggt cattaccggg
     2581 cctgatatcc catttcctgt ggagtctgac caaatattcc tttattttcc ttattctgat
     2641 gatgatgggc gtgtatttcc tgattcagct accctccttt cagaattacg ctgcaacaaa
     2701 agcaacagag tttctaagcc gtgaacttaa gtctgaagta acagtcggaa gaataagaat
     2761 taatttcttt cagaaagtcc tgcttgaaga ctttgtcgta aaagacagac aacaagacac
     2821 tttactgaat ataggggaat tgaaggctga attgaaaggt ggctttttgg gcctcctcga
     2881 tggtaaaatt aatatcacat ccgcattcat cagaaatctg gatgcccacc tcgtagaaaa
     2941 ttgcccgaaa tatgataata attaccaatt cctgctcaat tatctcgaaa atgggcac
//
LOCUS       DAOQMC010000270         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_18653_length_2988_cov_4.168769, whole genome shotgun sequence.
ACCESSION   DAOQMC010000270 DAOQMC010000000
VERSION     DAOQMC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18653_length_2988_cov_4.168769"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKV31_09250"
     CDS             <1..253
                     /locus_tag="PKV31_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNB93084.1"
                     /translation="DKRIVGLATIVEEAYPDPTDETGKWVAVKVAYLKPLNKPIEYKV
                     LKEMDHMKKFDLIRISRLSVMPVPDEFWYEILEMSETTV"
     gene            257..646
                     /locus_tag="PKV31_09255"
     CDS             257..646
                     /locus_tag="PKV31_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93085.1"
                     /translation="MKNLLLLILGVSLFTSCKKTIISPPVTYKGIWQAGSFVVKDSSV
                     TLDVSNIILKLNEDSRYSFVNNIGQNEAGVYVVRDSLLVVTDTTVSPSVEKAMQITGL
                     TSDSLVLRMNFGGKESWMHFTKQKESK"
     gene            complement(740..2365)
                     /gene="pruA"
                     /locus_tag="PKV31_09260"
     CDS             complement(740..2365)
                     /gene="pruA"
                     /locus_tag="PKV31_09260"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNB93086.1"
                     /translation="MSNAIFKTPRPVNEVVRSYAPGTEDRKQLEAAIKEAKSKTHDIP
                     MYIGGKEVRTDNKVAIRPPHEIKHTLAYYHKGDENHVKAAIDAALGAKAQWSSMPWEH
                     RAAIFLKAAELIAGPYRMKINAATMLGQSKNAFQAEIDSACELIDFLRFNVEYMTEIY
                     KIQPESSPGIWNRLEWRPLEGFVFALTPFNFTAIAGNLPSSPALMGNTVVWKPSETQI
                     FAANVIMEVFREAGLPDGVINLVFVSGAVAGDVIFSHRDFAGLHFTGGTSTFNTIYRK
                     IASHLDDYRSYPRIVGETGGKDFVMVHKSANPDEVAVALARGAFEYQGQKCSAASRAY
                     IPRSLWPAIKERLQKDVESMKMGSTEDFSNFINAVIDKKAFDFITSYIDRAKQSDVAK
                     VIIGGEYDDSEGYFIRPTVIETTNPKYDTLCTELFGPVLTVYVYEDDQFQETMKLLDE
                     TSDYALTGSIFAKDRYAIDLAVKALENSAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSVLNLYRWVSARTIKETFVPPTDYRYPFLG"
     gene            complement(2464..2919)
                     /gene="coaD"
                     /locus_tag="PKV31_09265"
     CDS             complement(2464..2919)
                     /gene="coaD"
                     /locus_tag="PKV31_09265"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNB93087.1"
                     /translation="MKIAVFPGSFDPITVGHFDLIVRSLALFDKVIVAIGINDAKSYL
                     YPLEKRMEWLRIVFAEYDKVEVDHYRGLTADFCKKVKANFLVRGLRNSSDFDYEKTIS
                     QVNYIITGGIETIFLISAPKYSHISSTIVREVIKGGGDVKPFLPKGVVL"
BASE COUNT          831 a          645 c          619 g          893 t
ORIGIN      
        1 cgataaaaga atcgttggcc tggctacaat tgttgaagaa gcttatcctg atcctaccga
       61 tgaaaccgga aaatgggtag cagttaaggt tgcttacctc aaaccgctca ataaaccgat
      121 tgagtataag gtgttgaaag agatggatca catgaaaaag ttcgatctga tcagaatatc
      181 caggctttcc gtaatgcctg ttcccgacga attctggtac gaaatccttg aaatgtcaga
      241 aactaccgtt tgatcgatga agaatctgtt acttttaatt ttgggcgtct ctttgttcac
      301 ctcttgcaaa aaaactatta tatccccccc tgttacttac aagggaattt ggcaagcagg
      361 atctttcgta gtgaaggata gttctgttac cttggatgtc agcaatatca ttctaaaact
      421 gaatgaagat tcccggtaca gttttgtaaa taatattggt cagaatgaag caggtgtcta
      481 tgtagtaagg gatagtcttc tggttgtcac cgacacaacg gtatctcctt cagttgaaaa
      541 agccatgcag atcaccggtt tgacttcaga cagcctggta ttgcgtatga attttggagg
      601 aaaagaatcc tggatgcatt tcaccaaaca aaaagaatca aagtaaacct aaccctgcat
      661 tttattattt tcatagaata ggaacaaaaa tgggatgcca taagcagccc atttttttat
      721 caaataatct tgttttcaat tatccaagga agggatatct gtaatcggtt ggcggaacga
      781 atgtttcctt gatggtcctg gctgataccc atctgtaaag gttaagtaca gaaccggctt
      841 tgtcattggt acctgaccct ctggcccctc caaatggctg ttgacctaca acagcgcccg
      901 ttggcttatc attgatgtag aagttgccgg cactgttttc aagtgctttt actgccagat
      961 caatcgcata cctgtctttt gcaaagattg agcccgtcag tgcataatca gaagtttcgt
     1021 ccagtaattt catggtctct tggaattgat catcttcata tacatatacc gttaataccg
     1081 gcccgaataa ctcagtacaa agggtatcat atttaggatt ggtagtttcg atgacagtcg
     1141 gtctgatgaa gtatccttct gaatcgtcat actcaccgcc gataatgacc tttgctacat
     1201 cagattgttt agctctatct atataagatg tgataaagtc aaaggctttc ttgtcgatta
     1261 cggcgttgat gaaattggag aaatcttcag ttgaccccat tttcatagac tctacatcct
     1321 tttggagcct ttctttgata gcaggccata aggaacgggg aatatatgcc ctggatgctg
     1381 ctgagcattt ctggccctga tattcgaatg cgccacgtgc aagtgccact gcaacctcat
     1441 cagggttagc acttttgtgt accattacaa agtccttgcc tcctgtctca cctactattc
     1501 ttggataaga acggtaatcg tcaagatgac ttgcaatttt tctgtatatt gtgttgaaag
     1561 tcgatgtgcc cccggtaaag tgcaacccgg caaaatccct gtggctgaat attacatcac
     1621 ctgccaccgc tccggaaaca aagacaaggt tgatcacacc atctggcaat ccggcttccc
     1681 tgaatacttc cataatgaca ttggccgcaa atatttgggt ttcagatggc ttccatacta
     1741 ccgtattgcc cattagtgca ggagagcttg gcagattccc ggctatcgca gtaaagttga
     1801 aaggagtcag ggcaaataca aaaccctcta atggcctcca ttccagtctg ttccagatcc
     1861 cgggcgatga ttccggctgg attttatata tttctgtcat atactcgaca ttgaatcgca
     1921 ggaagtcaat taattcacag gcactgtcaa tctcagcctg aaaggcattc ttggactgtc
     1981 ccagcatagt tgcagcattg atcttcatac ggtaagggcc ggcaatcaat tcggctgcct
     2041 taaggaagat ggctgctcgg tgttcccagg gcatactgct ccattgggct tttgctccaa
     2101 gggcagcatc tattgcagcc ttgacatgat tttcatcccc tttatggtaa taggcaagcg
     2161 tatgctttat ttcatgtggt gggcgtatcg caactttatt atctgtcctg acttcttttc
     2221 caccaatata cattgggata tcatgggtct tactcttggc ttccttgatg gcagcctcca
     2281 gttgcttcct gtcttcagta ccgggagcgt atgaacggac gacctcattg accggccgtg
     2341 gagttttaaa tattgcgttt gacataaaaa tggtttataa tttatgataa ttaattaagg
     2401 tcgtgtgagg caattgatac atatgaacga gaagaaccga acaaaaggaa taatcgtcat
     2461 ttcttataac acaactcctt tgggcaaaaa tggtttaaca tccccgccac ctttaatcac
     2521 ctctcgcacg atcgtggaac taatatgaga atacttgggc gcactaatga ggaagatggt
     2581 ttcgattcct cctgtgatga tgtaattgac ctgagagatg gtcttttcat aatcaaagtc
     2641 tgatgaattt cgaagtcccc tgactaaaaa gttagctttg acttttttac agaaatctgc
     2701 tgtcagcccc cgataatggt caacctcaac cttatcatat tcagcaaaga ctatacgtag
     2761 ccattccatt cttttttcaa gcggatacaa atatgacttg gcatcattga tgccaatagc
     2821 gacaataacc ttgtcaaaaa gtgccagact gcggacaatc aagtcaaaat gtccaacggt
     2881 aatcggatcg aaagagcccg gaaatactgc tattttcatc aaacgttact ttcaggataa
     2941 tggttattca acatcatttg atatcgaaaa atatgttgcg ttttttgc
//
LOCUS       DAOQMC010000271         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_18894_length_2967_cov_4.165179, whole genome shotgun sequence.
ACCESSION   DAOQMC010000271 DAOQMC010000000
VERSION     DAOQMC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18894_length_2967_cov_4.165179"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1854
                     /locus_tag="PKV31_09270"
     CDS             238..1854
                     /locus_tag="PKV31_09270"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNB93088.1"
                     /translation="MASLELRKGILGERLAGHLLRRATYRYDIGRIKEFAQYTASEAV
                     DKLMLPVTFTLDQPIDPATGAPWINNGTSTTTAQPALRNYVRSWVLNEMRFDETIHGK
                     MVFFLHKCLVTTYNQSTSERFFDYLALLYKFTTGSFKVLARKMTMDNLMLIYLNNTNN
                     NKNAPNENYAREFLELFTIGKGPQVGPGDYTNYTEEDVVVGAKLLTGIRANTRLATID
                     PDTGLSTGRFAFGQHNTEDKTFSAAFDNRTIKGAKSVDEMPRELEEYVDMVFSKIETA
                     RLYCRRLYRYFISSNITPEIESDIIHPLAQQLMDNNYETASAIKTLLTSVHFYDEDDS
                     DNKDEIIGALIKSPLELFLGSVNFLHQKWPDQTTETASLYNFINRLAIAVILDNGALP
                     FFAPYDVAGYSAYYQEPVYDESWFTANTIVSRYKLCEMLLTGTSYLGGKSTKVTLDLP
                     AFVRNSGFFSNPGDPIQLTKEMANGMLPEPPDDDRINYFLNEMLDGIPPADWTYEWVA
                     YTSSGDPENVKIGLSKLLTAIMYSQEYQLL"
     gene            1884..>2967
                     /locus_tag="PKV31_09275"
     CDS             1884..>2967
                     /locus_tag="PKV31_09275"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNB93089.1"
                     /translation="MKRRNFLRISTPAVLGPLVLEKSILKPFATGSLARIFNCEDVND
                     RILMIVQLKGGNDGLNCIVPTNQYDTYKNFRPTIGTSLDQLIPLDNTLSNDDHISLHP
                     GLISFKELYDQGCFTLVQAVGYANSNKSHFKGTDLWLSGGDSTPAHFNYKSGWMGRYL
                     DASYPGLAGEPTVDHPDPLGIQLGSKQQFLGFHTEHQHEAGINLSGQDPAGFYSLVSE
                     LGGIAPAHIPTSDYGNNIEYIIDIEHSTSRYSNRISEVFKKGINMGTYPDYDLADQLK
                     TVARMISGGGKTKIYVVSIGSFDTHQNQVEGSNPLDGTHGKLLTQLSESILAFQNDLR
                     MMQLDDRVVGVTFSEFGRRPKENGSKG"
BASE COUNT          908 a          660 c          616 g          783 t
ORIGIN      
        1 gtttgaagaa aaaacaagtc gcaaagatat atattttcca tttcaatttc aagagaaata
       61 atatttcaaa atatttgata ttacaggaat agtattttaa ttttaccgtt gctataacgc
      121 aaaagaaaaa tatttacctg gtcattaaac tctttcactt ttagcatatc aaatcaattg
      181 ttgacaggga aattttcacc cttcaagcat caatttgtta acagataaaa tcttattatg
      241 gcttctttgg aattaagaaa aggcatcctg ggagagcggc tcgcaggtca tcttctaaga
      301 cgggctacct accgttatga tatcggcagg ataaaggaat ttgcccaata tactgcatca
      361 gaggcagtgg acaaactgat gttgccggtc acatttactc tggaccaacc catcgatcca
      421 gccaccggag caccatggat caacaatggt acaagtacga ccacagcaca acccgccctc
      481 cgcaactatg tacgatcctg ggtgctcaac gagatgagat ttgatgaaac cattcatggc
      541 aagatggtct tttttctaca taaatgcctg gtgacaacct ataatcaaag tacgtcggaa
      601 agatttttcg actatctggc attattgtac aaattcacga caggtagctt taaggtttta
      661 gcaaggaaga tgacgatgga caatcttatg ctcatctatc tgaacaatac aaataacaat
      721 aagaatgccc ccaatgagaa ctatgccaga gagtttctgg agctgtttac cattgggaaa
      781 ggaccacagg taggtcccgg cgattacacc aattatacag aagaagatgt cgttgtggga
      841 gccaaacttc tgaccggaat cagagccaat acaaggctgg ccaccatcga ccccgacacc
      901 ggattgtcaa ccgggcgatt tgctttcggc caacacaaca ccgaggataa aacgttcagt
      961 gcagccttcg acaaccgcac catcaaaggc gctaaatcag tggatgaaat gccaagagaa
     1021 cttgaagaat atgtggacat ggtcttcagc aagatagaga cggcacgact atattgtcgt
     1081 cgcttataca ggtatttcat ttcatccaat atcacccctg aaattgaatc cgatatcatc
     1141 catccattgg cgcagcaatt gatggataat aattatgaga cagcctcagc aataaagacc
     1201 ttactaactt ctgtccattt ttatgatgag gatgattcag acaacaagga tgaaataatc
     1261 ggtgctttga taaaaagccc tctggaactc tttcttggca gtgtaaactt cctccatcaa
     1321 aaatggccgg atcagactac agaaacagcc agtttgtaca actttatcaa tagattagca
     1381 atagctgtga ttctggacaa tggtgcactg ccttttttcg ctccatacga tgtggcaggc
     1441 tattcagctt attatcagga acctgtgtat gatgaatcct ggtttactgc caatactatc
     1501 gtttctaggt acaaactatg tgaaatgttg ttaaccggga catcctatct tggaggcaag
     1561 agcaccaagg taaccctgga tttacctgcc tttgtccgga attcaggctt tttctccaat
     1621 ccaggagacc ctatacagtt aacgaaggaa atggccaatg gcatgttgcc cgaaccaccg
     1681 gatgatgacc gaatcaatta tttcctcaat gaaatgctcg atggaatacc gcctgccgat
     1741 tggacctacg aatgggtggc ctatacaagc agcggcgatc ctgaaaatgt taagatcgga
     1801 ttgtccaaac tccttacggc aatcatgtat tcccaggaat accaattgtt ataatcctat
     1861 tattctggca taaatcaata atcatgaaaa gacgtaattt ccttcgaata tcaacacctg
     1921 cagtcttagg tcctttagtt cttgaaaaaa gtatcctaaa gccatttgca acaggatcac
     1981 ttgcccgcat cttcaattgt gaggatgtca atgaccggat cctgatgatt gtccagctta
     2041 agggaggcaa tgatggcctc aactgtattg taccaactaa tcaatatgac acgtataaaa
     2101 actttagacc taccattgga acctctttag accaattgat tcccctggac aatacattat
     2161 ccaatgatga ccacataagc cttcatccgg gcctgatttc ctttaaggaa ctctacgacc
     2221 agggttgttt tacccttgtt caggctgtag gttatgccaa ttcaaacaaa tcacatttca
     2281 agggaacaga cctttggcta tcaggagggg acagcacacc ggctcacttc aattacaaaa
     2341 gcggatggat gggccggtat ctcgatgcat cctatcccgg actggcagga gaaccgacag
     2401 tagatcatcc tgatccatta gggatacagc taggaagtaa acaacagttt ctgggttttc
     2461 atacagaaca ccagcacgaa gccggaatca acctttcagg gcaggatccg gccggtttct
     2521 attcattggt ttccgagtta ggcggtattg cacctgcaca tattccgact tctgattatg
     2581 gcaataatat tgagtacatc atcgatatag aacattctac aagtcgctac tccaaccgta
     2641 tatccgaagt attcaagaag ggtatcaata tgggtactta tcctgattac gatttggcag
     2701 atcagctcaa gacagtggca aggatgatta gtggcggagg gaagaccaag atttatgtgg
     2761 tgtctatcgg cagcttcgat acccatcaaa atcaggtaga gggatccaat ccgcttgatg
     2821 gcacacatgg caagctcctt actcagcttt ctgagtccat ccttgctttc caaaacgacc
     2881 tgaggatgat gcagcttgac gacagggtcg taggagtgac cttctctgaa ttcggaagaa
     2941 gaccgaaaga aaatggctca aaaggca
//
LOCUS       DAOQMC010000272         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_19537_length_2911_cov_6.418417, whole genome shotgun sequence.
ACCESSION   DAOQMC010000272 DAOQMC010000000
VERSION     DAOQMC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19537_length_2911_cov_6.418417"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKV31_09280"
     CDS             <1..333
                     /locus_tag="PKV31_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93090.1"
                     /translation="PDQLISVVAMQWAWKMAEGPMDKNPQEFEGTNEITVPSGKLIQF
                     AVTSMDVNHNFAVYNSKGDIVTQTQAMPGYTNRLNFRFQEPGVYHVLCLEYCGLAHGF
                     MVGKINVN"
     gene            347..1828
                     /locus_tag="PKV31_09285"
     CDS             347..1828
                     /locus_tag="PKV31_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB93091.1"
                     /translation="MKELSSIEINGHRKLVKIWAITFLVLFPVLITLGLLMRLNQGEV
                     LDIGIDNFYVFMTLHGLGMAGVMYSMAFAGLWYLLSKKYVRLNLKTGYFVYFAVLIGV
                     VGVTISTLIGKFGAGWYLLYPLPFKGTFWPGWSTLLIIISLVILGVAWLIGNIHVVFA
                     LAREYGGLGNILGWQYFGKKEPERELPPVVLISAVSLIPGILAFIAGAIFLIMYLLQN
                     FEPGLTFDNLLVKNLIFFFGHTLVNITLYCCLGWVYTIMPEYTHREWKVNKLLVWAWS
                     GTFFFILFAYFHHLYMDFAQPKGMQYAGQLASYFSAIPATAVTMFGMIAQVYHAKLKW
                     SIVPLAFLMGVAGWAIGGFAAIIDSTIALNKILHNTLWVPAHFHTYMLGGVVLFILGY
                     LFYITHSKEELLKNNMAKFGFWSFLLAAHGFLAFFYSGGLYSIPRRYSDYKGIPMEET
                     HNIGANIAAVSTIFISFVLIGLLIMYVSLFKKILAKNTQDGII"
     gene            1873..2496
                     /locus_tag="PKV31_09290"
     CDS             1873..2496
                     /locus_tag="PKV31_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB93092.1"
                     /translation="MRNVFIPILSLIICLTVIGVWTLGFSSFTIFSYTLKKAGELPRP
                     LPDIQMINHEGIVFNPGEIKKYKLINFVYLNCPFVCHKINNRLEQIYNKIDTNLVPNE
                     IEFLTVSFDLKNDDVKKIKNYHKLFKGEISGWTFALPYQSTDRYFTDFLSKLGIWKYT
                     VPSTGLINHSIYIFLVNRQNQIVRVFDPARESDEMIIQGITQCLKEK"
     gene            2508..>2911
                     /locus_tag="PKV31_09295"
     CDS             2508..>2911
                     /locus_tag="PKV31_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93093.1"
                     /translation="MSLLGIMIWLLSFTLNEWLSETMPRHQLIQLPSMFFIGVLMGIT
                     ASHHLKPDLPWSIAVLIFVMFSLLFWMIPHSIDYAVISPGFNRIMHANMLLAGFLTIS
                     SIRHTIPEVKIIFLGMVAAMIIAGGIALLSFN"
BASE COUNT          791 a          606 c          612 g          902 t
ORIGIN      
        1 cctgatcaat tgataagtgt tgtggctatg caatgggcct ggaagatggc tgaaggtccg
       61 atggacaaaa atccacaaga atttgaaggc accaatgaaa taacagtccc ttccggtaag
      121 ttgatccaat ttgctgtaac gtccatggat gtcaatcaca actttgccgt gtacaatagc
      181 aaaggcgaca ttgttactca gactcaggcg atgccgggtt ataccaacag gctcaacttc
      241 agatttcagg aaccgggtgt ctaccatgtc ctttgcctgg aatattgtgg tttagcccac
      301 ggttttatgg ttggaaaaat aaacgttaat taaaaaaaag catctgatga aagaattaag
      361 ttctatcgaa ataaatggac atagaaagct cgtcaagatc tgggcaatta cctttctagt
      421 gcttttcccg gtattgatca cacttggtct tctcatgcga ctcaatcaag gtgaagtctt
      481 agatataggt atcgacaatt tttatgtatt catgaccctg catggcctgg ggatggctgg
      541 agtgatgtac agcatggcat ttgccgggct ttggtacctg ttaagtaaaa aatatgtcag
      601 actaaacctg aagaccggct attttgttta ttttgcggta ttaataggtg tagttggggt
      661 caccatctcc accttaatcg gaaaatttgg tgccggctgg tatcttttgt acccgcttcc
      721 attcaaagga actttctggc ccggctggtc cacccttttg atcatcattt ctcttgttat
      781 tttaggagtt gcctggctga taggaaatat ccatgttgtg tttgccctgg ccagggaata
      841 tggtggcctt ggcaatatac ttggctggca atattttgga aagaaagaac ctgagaggga
      901 attacctccc gtggtgctta tttctgcagt aagtcttatc cccggcatac tagcctttat
      961 cgccggtgca atcttcctga ttatgtatct gttgcagaat tttgaaccgg gattgacttt
     1021 tgacaatctt ttagtcaaga atctgatttt ctttttcggg cacacgcttg tcaatataac
     1081 gctatattgc tgtcttggat gggtctatac cataatgccg gaatatactc acagagaatg
     1141 gaaagtcaac aaactgttgg tttgggcatg gagtgggact ttctttttta ttttatttgc
     1201 atatttccac catttatata tggattttgc tcagccaaag ggaatgcaat atgcagggca
     1261 actcgcttcg tatttcagtg ccatccctgc cacggcagta actatgttcg gcatgattgc
     1321 ccaggtgtat catgccaagt taaaatggag catagttcca ctggccttcc tgatgggagt
     1381 ggcaggatgg gcgattggag gatttgccgc aatcatagac tctaccattg cattgaacaa
     1441 aatcctccat aatacccttt gggtgccggc tcatttccat acctacatgc taggaggagt
     1501 ggtacttttt attttaggat acctattcta tatcacacac agtaaggagg aattgctcaa
     1561 aaacaatatg gccaaatttg ggttctggtc cttcctgctg gccgctcacg gctttctggc
     1621 attcttctat tccggaggat tgtatagcat accaaggagg tactccgact ataaaggcat
     1681 accaatggaa gaaacccaca atataggtgc caatatagca gccgtttcga ccatatttat
     1741 cagttttgta ttgattggat tattgattat gtatgtttct ttgtttaaga aaatactggc
     1801 gaagaatacg caggatggaa taatctgaca cctgcttctg agtaattttt cacagcatta
     1861 tcctgtcagt agatgagaaa tgtatttatt ccaattcttt ctttaatcat ttgtctgaca
     1921 gtaataggag tctggaccct gggtttttca tccttcacca ttttctcata taccttgaaa
     1981 aaggcaggtg aattacccag gcctcttccg gatatccaga tgatcaatca cgaaggtatt
     2041 gtttttaatc cgggtgaaat aaaaaagtac aaactcatca actttgttta cctcaactgt
     2101 ccttttgtct gtcacaagat caacaaccgc ctggagcaaa tttacaataa gattgatacg
     2161 aatttagttc cgaatgagat cgagttcctg actgttagtt ttgatctgaa gaacgacgat
     2221 gtaaagaaaa tcaagaatta tcacaaacta tttaaaggcg aaatatccgg ctggaccttc
     2281 gccctaccct atcaaagtac tgaccgatat tttacggact tccttagtaa gttgggtatc
     2341 tggaaatata ccgtaccatc taccgggttg atcaatcact ccatatacat cttcctcgtc
     2401 aatcgacaaa atcaaatagt aagggtcttc gatcccgcca gggaaagcga tgaaatgatc
     2461 attcaaggaa ttacacagtg cctgaaagaa aaataaagat tgcgatattg agcctcttag
     2521 gtataatgat ctggctcctg tcattcactc tcaacgaatg gctgagtgag actatgccca
     2581 gacatcaatt gattcaatta ccatcaatgt tcttcatagg tgttttaatg ggaattacgg
     2641 catcccatca cctaaagccg gatttgccat ggagtattgc tgtattgatc ttcgtaatgt
     2701 tctctctctt gttctggatg attccccatt cgatagatta tgctgtcata agccctggat
     2761 tcaaccgtat catgcatgcc aacatgcttc tggcaggctt ccttaccatt tcttccatac
     2821 gacacaccat tccggaagtt aaaatcatct tcctgggtat ggtggcagct atgatcatcg
     2881 ccggcggcat tgctttgctg tccttcaata t
//
LOCUS       DAOQMC010000273         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_19800_length_2889_cov_5.024347, whole genome shotgun sequence.
ACCESSION   DAOQMC010000273 DAOQMC010000000
VERSION     DAOQMC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19800_length_2889_cov_5.024347"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKV31_09300"
     CDS             <1..1113
                     /locus_tag="PKV31_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93094.1"
                     /translation="DYPLQYIHVGGDEVKIENKDFLPDIVAMIQGRGLRTIAWSPGGE
                     TNSHTMRQLWMDDDGHNNKAGELMYIDSRHLYLNHMDPLEAVTTIYYRKIGERTRGDT
                     AFPGATLCLWNDRAVADGSDILSMNPVYPGMMAFAERVWNGGGRSGWIAGIPLDDVPV
                     FSKFESGLLDHKALYFNNLPFPYVRQSNLRWTLIGPYANGGDFSIRFEPEISGKPGTG
                     RMIPAVGGTIVFRHWWAPQISGALQNPEENTTWYATTTIQSDVDTVRGFWIGFNDLSR
                     SPATDSPPLGGWNHRQGKLWVNGHEVPPPIWRHPGQQGNSEIPLVDEGYSYRPPTMIH
                     LKKGANTVLVKSPIGKFRGKDWQNPEKWMFTFVPVQ"
     gene            complement(1351..2292)
                     /locus_tag="PKV31_09305"
     CDS             complement(1351..2292)
                     /locus_tag="PKV31_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB93095.1"
                     /translation="MEDRTSIFPQFKHADWRKGFSREVGEAHLNEMNFTIDGLSFEPM
                     GDDEDAESFAGKLPKKEENIAFGEIFDFTGGNQPIRQLMESLQHDYTAPLLLVDKKTN
                     AGIFDDVRFDILNASIHVTDGWMDPWKVYFMILHGGYPKGLRIINRKDDSGKDALKHF
                     HFDAKNEVIPFIRQISREIEMTNHQNIQISMTFTGRTLHDIIKARAVKTSWFNILYHA
                     GLPLTPFHFEVILPVEGQYPDIVIGHTNNLIASTMAQADTVVFACRQDDNERRILRNS
                     AHISVIEAGLDKETDPVAGSYFVEKVAAQLAEKALAG"
     gene            2407..>2889
                     /locus_tag="PKV31_09310"
     CDS             2407..>2889
                     /locus_tag="PKV31_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB93096.1"
                     /translation="MMSKRHIYLDNNATTPTDPRVIEAMLPYFYEKPGNPASRNHPFG
                     WIAEEAVDYAREQIADLIHVDPKEIIFTSGATESDNLALKGVFEMYRSKGNHIVTAKT
                     EHKAVLDSCRKIEKMGGEVTYLDVERDGRIDLDKLEAAIKPETILVSIMWANNETGVI
                     Q"
BASE COUNT          789 a          585 c          707 g          808 t
ORIGIN      
        1 gactatcccc tgcaatatat tcatgttggt ggagatgagg tgaagattga aaataaggat
       61 tttcttccgg atatcgtggc gatgatccag gggcggggat tgcgtaccat tgcctggagt
      121 ccgggcgggg agaccaattc ccatactatg cggcagttgt ggatggatga tgacggtcac
      181 aacaataagg ccggagagtt gatgtatatt gattcaagac atctttacct caatcacatg
      241 gatccgttgg aggctgttac tacgatttat tacagaaaga ttggcgagcg gacaaggggt
      301 gataccgcct ttcccggagc tactctttgt ttgtggaacg atcgggctgt agcagatggt
      361 agcgatattc tgagcatgaa tccagtatat ccgggcatga tggcttttgc agagcgggtt
      421 tggaatggag ggggtcggtc cggatggatt gcaggcattc cgctcgatga tgtaccggta
      481 ttcagcaagt ttgaatcagg actgttggac cataaagcat tgtatttcaa caatcttccc
      541 tttccatatg tcaggcaatc gaatctccgc tggaccctca ttggtcccta tgccaatggt
      601 ggtgacttct cgatcaggtt tgaaccggaa atttcaggta agccgggtac cggacggatg
      661 attcctgctg ttgggggcac tattgttttc cgccattggt gggcgcccca gatcagcgga
      721 gcgcttcaga atccggaaga aaacacgacc tggtatgcga caacaaccat acaaagcgat
      781 gtggacacgg tgcgcggatt ttggattggt ttcaatgact tatcgagatc tccggctacg
      841 gatagcccgc cactcggagg atggaatcat cgacaaggta agctttgggt caatggtcac
      901 gaagtgccac cgcctatttg gcgtcatccc ggccagcaag gcaattcgga aattccgctc
      961 gtggatgaag gttacagtta caggccaccg acaatgattc atcttaagaa aggtgcaaat
     1021 accgtgctgg tcaagtcacc gataggcaag ttcagaggaa aggattggca gaatcctgaa
     1081 aaatggatgt ttacctttgt tccggtacaa taatacgact gcagtttgtc ttgaagaccg
     1141 gagatattgt accaatgaat atatggggaa atagcagaaa tttctgagct acaattgtat
     1201 tggtatgtat gttgggttac agcgggttaa tattatcctg tttgattgtt gtggtataaa
     1261 aatttcactt ttcaccgact gtctttttgg tgtaaattcc gcaaaagttg tgaatatggt
     1321 aaaaaaaata aagtagggcc tgttggtgaa ttatccggcc agagcctttt cggccaactg
     1381 cgcggctacc ttttccacga aataactacc ggcaacggga tccgtttcct tgtcgaggcc
     1441 tgcttcaatg actgaaatat gggcagaatt tctcaagatt cgtcgctcgt tgtcatcttg
     1501 cctgcaagcg aatacaacgg tatcggcctg agccattgtt gaagctataa gattattggt
     1561 atggccgatg acaatatcag gatattgacc ctcaaccggc agaatcacct caaaatgaaa
     1621 gggtgtcaaa ggcaaaccgg catgataaag gatattgaac caggatgttt ttactgctct
     1681 tgccttgata atatcatgta aggtgcgccc ggtgaatgtc atggaaatct gtatgttttg
     1741 atgatttgtc atctctattt ctcttgaaat ttgccggatg aatggaatga cttcattttt
     1801 tgcatcaaaa tgaaaatgct tcaaggcatc ttttcctgaa tcatcctttc gattgatgat
     1861 acgtagccct tttggatatc ctccgtgtaa tatcataaaa taaaccttcc aggggtccat
     1921 ccagccgtct gtgacatgta tggaagcatt caggatatca aaccggacat catcaaagat
     1981 cccggcattg gtcttcttat ccaccaaaag caacggagcg gtatagtcat gttgcaatga
     2041 ttccatcaat tgccggatcg gttgatttcc tccggtaaaa tcgaaaattt ctccaaatgc
     2101 gatattttcc tctttctttg gcaactttcc ggcaaaactt tctgcatctt catcatcacc
     2161 cattggctcg aatgagaggc cgtcgatggt aaaattcatt tcattcagat gcgcttctcc
     2221 cacttccctg gagaatcctt ttctccagtc ggcatgctta aattggggga aaatagatgt
     2281 cctgtcttcc attacaaaaa tttctaatag gagcaaaggt agaaacaaat acactggaat
     2341 tgttgaactt aatacttttt ttgtagttat atttgcacgg attttattta gattatttaa
     2401 aaataaatga tgagcaaaag acatatatat cttgataata atgcaacaac gcctacagat
     2461 ccgagagtta tagaggctat gttgccttat ttttacgaaa aaccgggaaa tccggctagc
     2521 cgtaatcacc cttttggctg gattgcagaa gaggcagttg actatgcgag ggagcagatt
     2581 gcggatttga tccatgtgga tccgaaggaa ataatcttta cttcaggggc caccgagtcg
     2641 gacaatcttg ccctgaaagg tgtttttgaa atgtatcgtt ccaaggggaa ccacatcgtt
     2701 accgccaaga cagagcataa ggctgtactt gacagttgca gaaaaattga gaaaatgggc
     2761 ggtgaggtga cctaccttga tgtggaaagg gatgggcgca tagatcttga taagcttgag
     2821 gctgctatca aaccggagac aatcttagtc agcattatgt gggcaaacaa cgaaaccggg
     2881 gtaattcag
//
LOCUS       DAOQMC010000274         2875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_19966_length_2875_cov_5.349645, whole genome shotgun sequence.
ACCESSION   DAOQMC010000274 DAOQMC010000000
VERSION     DAOQMC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19966_length_2875_cov_5.349645"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="PKV31_09315"
     CDS             <1..1289
                     /locus_tag="PKV31_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93097.1"
                     /translation="CGWMPKGVELHITVRPVICPDKAKEVVVGPFYNNASINNIVVDA
                     QKKPVSVKVTCNGVPAGDAIVVLEQNGKTILVDVPNGASTFNLYNYTCDDNSPISIFG
                     FEQKTGKTSSDQAFNFANGVYEFDVCQSGCSLSGVFITDCDPIIIKVSGGSGQYKYQW
                     NTGDTTDRFSIKSADAKLYSVTVTDIQNPTCSQVFTKNLPGLISCTIELTDCHWPISL
                     RVKGSNYNKIKWSNGHDGVSQDVQPDHTTTYSVTVTNEIGCSSTASIVVDPDLFPYVS
                     SARTCSKNLITLPGRFKSGTIQGFNGFSKNLNSPSDLVDLNIFETGYVLWGYVNNGKG
                     CEQPFEFNLPQYDGLKVLNFSTGNVIDGNIIKYTASNGSCYNCDAGDVAIFKASDLNT
                     DLVQLNATGLPGGEYYVVLKDANSGCFVAFVKVKII"
     gene            1291..1863
                     /locus_tag="PKV31_09320"
     CDS             1291..1863
                     /locus_tag="PKV31_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB93098.1"
                     /translation="MDIDNILQGCLQQQQSSQELLVHAFAPKLLSICERYCKDKEMAR
                     DAVQETFINIFKYIHTYNGETAFITWARKIAVNNCLKINKKYFKLQFVDEAEARFNHG
                     SYIPEAYSNLSVESIMELVRKLPDSQYVVFNMYVVEGFSHKEIGEALNITESSSRSTL
                     TRARSKLIEMINDIEDKNNNSIFNSRIALL"
     gene            1860..>2875
                     /locus_tag="PKV31_09325"
     CDS             1860..>2875
                     /locus_tag="PKV31_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93099.1"
                     /translation="MNYKEFEKNIKNKLSEHEAQIDTNALVSAIFLKKKKGKRFPAWW
                     GYVGALIVVTLVSTLWIFDSRNNDLSYKDRAATNPTAESEQPGLTYDDVYDRTPTDGL
                     NTKDNSVKNEMVASLASGNNLDVNSGTASFQDNQSTYNKALSGEDIQQASAITVSDVD
                     DSKIPVSNTLSEEKIVSSDGIQSTYSTSIARVKKGVSDIGVLTSKSVDLHLNNKALFD
                     LGPGVKCPTFKSKRRIGFALIPEVGVFYPLANFKSEAGPENQPFAMRERNERSLEGLQ
                     AGLYGKIFLSNSPWYLKTGLNYGRITRQMTFNDSYIQRDTTIGIIAITESQNHDTLTI
                     IKGP"
BASE COUNT          875 a          527 c          649 g          824 t
ORIGIN      
        1 attgtggatg gatgcctaag ggagttgagt tgcacatcac cgtccgaccg gttatttgtc
       61 ctgacaaggc gaaggaagtc gttgttggcc cgttttacaa caatgcgagt attaacaaca
      121 ttgtggttga tgcccagaaa aagccagtct ctgttaaggt tacctgcaac ggagttcctg
      181 ccggagacgc gatagtagta cttgagcaga atggtaaaac gatcctggtt gatgtcccca
      241 atggagcctc tacattcaac ttgtataatt atacgtgtga tgacaactcg cccataagta
      301 tattcggctt tgagcaaaag accggtaaaa cttcgtctga tcaggctttt aactttgcaa
      361 atggcgtata tgaatttgat gtttgtcaat caggatgttc attgtcaggt gtgtttatta
      421 ccgattgtga tccgattatt attaaggttt caggaggtag tggacaatac aaatatcaat
      481 ggaataccgg tgatactacg gatagattca gtattaaaag tgccgatgct aaattgtatt
      541 ccgtaactgt aaccgatatt cagaatccca cttgtagtca ggtgtttact aagaatctac
      601 ccggtctcat ctcatgcaca attgaattga ccgattgcca ctggccgata tcactcagag
      661 taaagggttc caattataat aaaatcaaat ggtctaatgg acatgatggg gtaagccagg
      721 atgtccaacc tgatcataca acaacctatt ctgtcaccgt aacgaatgaa attggttgct
      781 catccactgc ttctattgta gtagatcctg atttgttccc ctatgtgagt tcggcaagga
      841 cgtgcagcaa gaatctgatc accttaccag gtaggttcaa gtcgggaact attcagggat
      901 ttaatggctt ttcaaaaaac ctgaatagtc cgtctgatct ggtcgacctg aatatttttg
      961 aaaccggtta tgtactttgg ggctatgtga ataacggtaa gggatgtgag cagcctttcg
     1021 agttcaacct gccccagtat gatggtttga aggtgttgaa tttcagtaca ggaaatgtta
     1081 tagatggtaa tattataaag tacacagcca gtaacgggag ttgctataat tgtgacgcag
     1141 gtgatgtagc tatcttcaag gcgagcgatc tcaatactga tctggtacag ttgaatgcca
     1201 ccggcttgcc tggcggagaa tattatgttg tattaaagga tgcgaattcg ggatgttttg
     1261 tagcatttgt taaagtgaaa ataatatagg gtggatattg acaatatact ccaaggctgt
     1321 cttcaacaac agcaaagtag tcaagagctt ttggtgcatg catttgcacc aaagcttttg
     1381 tctatctgtg agagatattg taaggataag gaaatggccc gcgatgctgt gcaagaaacc
     1441 ttcattaata tttttaaata catccacacc tacaatggag aaacagcatt tataacatgg
     1501 gcaagaaaga tagccgtcaa taattgtctg aaaataaata aaaaatattt caagttgcaa
     1561 tttgtcgatg aagctgaagc ccgattcaat catggctcat atatcccgga ggcttacagt
     1621 aacctatcgg tggagtccat tatggaatta gtacgaaaac tgccggatag ccaatacgtt
     1681 gtatttaata tgtatgtggt ggaagggttt agccataaag agatcgggga agcattgaat
     1741 attacggaaa gtagttcgag atcgactctt acccgtgcac gcagcaagct tatcgagatg
     1801 attaatgata ttgaagataa aaacaataat tcgattttta attccagaat cgctctgtta
     1861 tgaattacaa ggaattcgaa aagaatatta aaaataagtt atctgaacat gaggctcaga
     1921 tagataccaa tgcgctggtt tcagcgattt ttttaaaaaa gaaaaagggc aaacgcttcc
     1981 cggcctggtg gggctatgtt ggggctttga ttgttgttac ccttgtttcg acgctctgga
     2041 tctttgattc gaggaataat gatctttcgt acaaggatag ggccgctacg aatcctaccg
     2101 ccgaatctga gcaaccgggc cttacttacg atgatgtcta tgaccggaca cccacagatg
     2161 ggctaaatac taaggacaac tctgtcaaaa atgagatggt ggcatcgcta gcctccggca
     2221 ataatttgga tgttaattca ggtacagctt cattccagga taatcaaagt acttacaata
     2281 aggctctatc cggtgaggat atccaacaag cttctgccat aacggtttct gatgtcgatg
     2341 attcaaaaat acctgttagc aatacgttaa gtgaagaaaa gatagttagc tctgatggca
     2401 ttcaatcaac ttattctact tcaatagcgc gtgttaagaa aggtgtcagc gacataggtg
     2461 tcctgacttc aaaatctgta gatttacatt taaacaacaa ggctttattc gatttgggtc
     2521 cgggagtaaa atgcccaaca tttaaatcaa aaagaaggat tggatttgcc ctgataccgg
     2581 aagtaggcgt tttctatcct cttgctaatt ttaagagtga agcggggccg gaaaaccagc
     2641 cttttgcaat gcgagagaga aatgaacgca gccttgaagg attgcaggcc ggcttatatg
     2701 gtaaaatatt tctttccaat agtccatggt atttgaaaac cggtttgaat tatggacgga
     2761 tcaccagaca gatgacgttt aatgattcct atattcaaag ggataccact atcggtatta
     2821 ttgctataac ggaatcacaa aatcatgaca ccctgaccat cattaaaggg ccgat
//
LOCUS       DAOQMC010000275         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_20014_length_2871_cov_3.294034, whole genome shotgun sequence.
ACCESSION   DAOQMC010000275 DAOQMC010000000
VERSION     DAOQMC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20014_length_2871_cov_3.294034"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..1337)
                     /locus_tag="PKV31_09330"
     CDS             complement(351..1337)
                     /locus_tag="PKV31_09330"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNB93100.1"
                     /translation="MSRVITLRDALREAMVEEMRLDPTVFLMGEEVAAYNGAYKVSKG
                     LLDEFGPERVIDTPIAELGFAGIGVGAAMNGLKPIVEFMTWNFAVLAFDQIVNNAAKT
                     LSQSAGQFGCPIVFRGPSGAAGQLAQQHSQTFESWMGNVPGVKVISCTDPADAKGLLK
                     SAIRDPNPVCMMESEIFYAEKGEVPEGEYLVPIGKAAVRREGSDVTLVSFNKQVKTCM
                     LAAEELAREGVSAEVIDLRTIRPLDIHTVIESVRKTNRIIVVDESWPMFGVSAEIAYE
                     VQKYAFDYLDAPVTRVNAADTSLGYSHVYVDEYMPNPAKVIKAVKEVMYIKA"
     gene            1515..>2871
                     /gene="argS"
                     /locus_tag="PKV31_09335"
     CDS             1515..>2871
                     /gene="argS"
                     /locus_tag="PKV31_09335"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNB93101.1"
                     /translation="MDILSVIQNMVSSAFKDNFQSDIAPANIPISLTKPEIKGDYTVT
                     LFPFAKELRTNPNEMGNRMKDALSGKYPEISSIDVVGGFLNFSLSDTYWVDVLHTFNP
                     SPKNEDIKQRFLIEFSSPNTNKPLHLGHVRNMLLGESITNILRELGHDVIKVQVINDR
                     GIAICKSMLAFQLFGEGQTPTQAGIKGDHYVGNLYVLFENKFKEEYKQWQEGPVAGDL
                     LEASKNPDDPGKFFADFKNQYFNTYSKLGLAARDLLNKWEAGDTETLNLWKMMNDWVY
                     EGMNVTYENMGISFDKNYYESNAYLLGKEAVKIGLDRGIFTRDADGSVWIDLTEKGLD
                     RKILLRSDGTSVYITQDIGLAIQRHEEHHFDKMVYVVADEQNYHFQVLFAILKEMGMD
                     WADNLYHLAYGMVELPSGKMKSREGTVVDADDLMAEVIGEAAASGKERGGLASLSVDA
                     QE"
BASE COUNT          882 a          608 c          572 g          809 t
ORIGIN      
        1 atatcaacga atggaaaaga ttttagtaga agaaagtccg gttatctttt tatattatga
       61 cgccatcact aatttcacat cacctatgat taaggattat atgcctaatc cccttaattt
      121 gttaaaagtt aaatatatta aaaaagattg ccggaaccca atcaagtgat atccatgtag
      181 aaagatttct tctataatta cctattactt tggttaacat accggtacaa taggaagttt
      241 tttggtaagc taacctgatt cagaaaattt accccaaaag ctatgataat ttggggtata
      301 aaaaaaacag gctcaaaaga acctgtttat atttattaac tcaaaaactt ttaagcctta
      361 atgtacatca cctcttttac tgcctttatc actttagcag gattaggcat atattcatcc
      421 acgtatacat gggaataacc aagtgaagta tcggcggcat tgaccctggt cacaggtgca
      481 tccagataat caaaggcgta cttctgtact tcgtatgcaa tttcagcaga gacaccaaac
      541 atcggccaac tttcatcgac aacaatgatg cgattggttt tcctgaccga ctcgatgaca
      601 gtatggatat caagaggtct gatggtcctc aagtcaataa cctctgcaga aacaccttcc
      661 ctggcaagtt cttctgcagc caacatacag gtcttaactt gtttgttgaa agaaacaaga
      721 gtaacatcac ttccttctcg cctgactgct gccttaccga ttggaacaag atattcgcct
      781 tccgggactt cacccttttc agcatagaaa atctcagact ccatcataca taccgggttg
      841 ggatctctga ttgcagattt tagaagtccc ttggcatcag ccggatctgt acaagaaatc
      901 acttttacgc cgggaacatt acccatccag gactcaaaag tctgactgtg ctgttgcgcc
      961 aattggccgg cggcaccgga tggtcccctg aacacaatcg gacaaccaaa ctggcctgcg
     1021 gattgactaa gtgtcttcgc tgcattattg acgatctgat cgaaagcaag cacagcgaaa
     1081 ttccatgtca taaattcaac aatgggttta aggccattca tggctgctcc gacgccgatt
     1141 ccggcaaacc caagttcagc aataggcgta tcaataaccc tttcaggacc aaattcatcc
     1201 aacaaaccct tactcacctt atatgcacca ttataagcag caacttcttc ccccatgaga
     1261 aataccgtcg gatcaagacg catttcttct accattgctt ctctcaatgc atcccgtaat
     1321 gttattactc ttgacattct atttttaata attttgccgc aaaggtaaca tattttcaat
     1381 acaagccaag gcagattttt ccaatattcc cctttttctt cctgtaattg gacctgccta
     1441 agttaggcca gccttatcct ttgctgtaaa aaaaatatgt aagtttgcac cctaattaaa
     1501 attaataagt acgaatggat attctttctg tcatacaaaa tatggtcagc tcagcattca
     1561 aggataattt ccaatccgat attgcaccgg ctaacattcc aataagcctg acaaagcccg
     1621 agataaaagg tgactatacg gttacactgt ttccatttgc caaagagtta agaacaaatc
     1681 caaatgaaat gggaaatcgg atgaaagatg ctttgtccgg caaataccct gaaatatcat
     1741 ccattgatgt cgtcggtggc tttctgaatt tcagcctttc tgatacttat tgggttgatg
     1801 tcctgcatac atttaatcca agccccaaaa atgaggatat caaacaaaga tttctgatcg
     1861 aattttcctc gcccaatact aacaaaccct tacatctggg gcatgtacgg aatatgcttc
     1921 tgggagaatc gataaccaac atccttcgcg agcttggaca tgatgtcata aaagtacagg
     1981 taatcaatga tagaggcata gcgatctgta agagcatgct ggcatttcaa ttatttgggg
     2041 agggtcagac acccacccag gccggcatta aaggcgatca ttatgttggt aacttgtacg
     2101 ttctttttga gaataaattc aaggaagaat acaaacaatg gcaggaaggc cctgtagccg
     2161 gggacctact cgaggcttca aagaatcctg atgatcccgg taagttcttt gcggatttta
     2221 aaaaccaata tttcaataca tacagcaaac taggccttgc tgcccgcgat ttgcttaaca
     2281 aatgggaagc cggtgacacg gaaacgctca atctgtggaa aatgatgaac gattgggtgt
     2341 atgaaggtat gaatgtgaca tatgaaaata tgggcatctc ctttgataaa aactattatg
     2401 aaagcaatgc ttacctattg ggtaaagagg ctgtcaaaat aggcctggat aggggaattt
     2461 ttacgcgtga tgctgacggt tcggtatgga ttgatttgac cgaaaaaggt cttgacagga
     2521 aaatcctgct tcggtcagat ggaacttcag tatatataac tcaggatatc ggtttggcta
     2581 tccaacgtca tgaagaacat cattttgaca aaatggtata cgtcgttgct gatgagcaaa
     2641 attatcattt ccaggtatta tttgctattc ttaaagaaat gggcatggat tgggcagaca
     2701 atttatacca tcttgcgtat ggcatggttg agttgccaag cggtaaaatg aaatccagag
     2761 aaggcactgt tgtagatgca gacgatttaa tggcagaagt aatcggtgag gctgctgcct
     2821 caggtaagga gagaggcggg ctggcttctc tatccgttga tgctcaggaa c
//
LOCUS       DAOQMC010000276         2818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_20693_length_2818_cov_5.346001, whole genome shotgun sequence.
ACCESSION   DAOQMC010000276 DAOQMC010000000
VERSION     DAOQMC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20693_length_2818_cov_5.346001"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKV31_09340"
     CDS             complement(<1..711)
                     /locus_tag="PKV31_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB93102.1"
                     /translation="MAKEAKGQFTEKLVDRLTVHFENDSYTLDEEYKAEINEFIRNLD
                     VVIPYVFRIQGHTDSIGSVQYNQRLSNRRSNTIKDYLLDLGIPEKLISLKGIGEMKPV
                     RPNSEEEGRAYNRRANIFVFEIQKYRLFRSKVILSGNKDNKAAVYIQTDEGMDSTFTD
                     DKGNFAFVIPENKNVTYGIFAKGYMFSTRTINTSRDLPIGDIVLNRIKPGEKVSLNNL
                     YFVGDQAILLPESQPELVN"
     gene            complement(996..>2818)
                     /locus_tag="PKV31_09345"
     CDS             complement(996..>2818)
                     /locus_tag="PKV31_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB93103.1"
                     /translation="TRDLDPRTVFLGSNLMRKYNYAIYHGDNLVESQGYIYPDNLNTQ
                     KVFTRGGNMVETTSNNRSEIASKAGDGYVIIAGKNLMGILKPVSLCSFLIVILLSIVI
                     SVAFLNNRFRILPAELPIFISGQFNLRQRIEYSIILVIVASFAIIAFVTGSYFNNLSL
                     RMEESLLSEKAFGIVSDIESSLGNGGLDSIPEKSLHMISANQQAGFSLYAIDGRERFN
                     TIYAKIRSKKQNLMPPLPYLNLYFDNQSVYFDFAGEKNTIRAVYVPVRKANNQKIGWL
                     AVPYPEAGVTSIFAASDFLGTLLNVYVFLLLVAGGLAFFVANSVTKPLVRLMDNLRKI
                     KLGKKNEQLTWDQQDEIGSLIHEYNKMIDQLEESTALLVKSEREGAWRDMAQQVAHEI
                     KNPLTPMKLSIQYLERKIGSVPQEEMEQVVKDTARTIIEQIDNLASIATEFSNFAKMP
                     APIFEYINFNELVSSIHELFRKKDDIQFNLYVPIDDVMVIADRAHLMRLLNNLVQNAI
                     QSIPPGRQGRIGLELKIVDKNAVLSVKDNGQGIPKDMHEKVFYPRFTTKSSGTGLGLA
                     MCKSIVDSFNGTISFKTQPDVGTEFIVSIPLAQDSHPEIP"
BASE COUNT          745 a          565 c          631 g          877 t
ORIGIN      
        1 attgaccagt tccggttgtg attcgggaag aagaatggcc tgatcgccca caaaataaag
       61 attgttgagt gatacttttt ctccgggttt gatcctgttt aagacaatgt ctcctatcgg
      121 caagtccctg gaagtattga tcgttctggt tgaaaacata taacctttgg caaagatacc
      181 ataggttaca tttttatttt caggtatgac aaaggcaaag ttccccttgt cgtcagtaaa
      241 agtggagtcc ataccctcat cggtttgaat atagactgct gctttgttgt ctttattccc
      301 gctaagaatg actttactac ggaatagtcg atatttttga atttcgaaga caaaaatatt
      361 tgctcttctg ttgtaggccc tgccttcctc ctcgctgttg ggtcttacgg gcttcatttc
      421 tccgattccc ttcagggaaa tcaacttctc cggaatacca agatcaagaa gataatcctt
      481 gatggtgttg gatcttcggt tagaaagcct ttgattgtat tgtacgctgc cgattgaatc
      541 ggtatggcct tgaatcctga agacataagg gatgaccaca tccagattgc ggataaattc
      601 attgatttcc gctttgtatt cttcatcaag cgtataggaa tcattttcaa aatgcacggt
      661 caggcggtca acaagttttt ccgtaaactg accctttgct tcttttgcca atattgttag
      721 taaaatgacg ggaatgatcc tgaataaatg cttattaaaa aaagataaca ttgcaacaaa
      781 caataaggtg ttcagaataa aaattactaa ggatagtttg aaaatcaacc atcttctatt
      841 ttattaattg gacaaaaatt tcgtgcgcaa acaggtagat tgacctactt aatcctaaag
      901 atataaaaaa tattgggatc cggtattttg ccgggagata ttttgttgtt acgggtttga
      961 atggtgtaac gataaaggtt ttaggtgatt ctgttttagg gaatttccgg gtggctgtcc
     1021 tgagccaatg gaatgctgac aataaattct gtaccgacat caggttgagt tttgaagcta
     1081 atggtgccat tgaatgagtc tacgattgat ttacacatag ccagacccag gcccgttcca
     1141 ctgctcttcg tggtaaagcg gggataaaat accttttcgt gcatatcttt aggaattccc
     1201 tgaccattat cttttacgga gagaactgca tttttgtcta caatcttcaa ctccagtcca
     1261 atccttccct gcctgccggg gggaatgctt tggatggcat tttggacgag attgttgaga
     1321 agtctcatca gatgggctct gtcagcaatt accatgacgt catctatagg cacgtagagg
     1381 ttaaactgga tatcatcctt ctttcggaat aattcatgga tggaagacac cagttcattg
     1441 aagttgatgt attcaaagat tggggcaggc atcttggcaa aattggaaaa ttctgttgcg
     1501 atacttgcaa gattgtcgat ctgctcgatg atggttcttg ctgtatcctt gacgacttgc
     1561 tccatttctt cctgcggtac gctgcctatc tttcgctcaa gatattggat gctcagcttc
     1621 atcggggtaa gcggattttt tatttcatgt gcgacctgtt gggccatgtc gcgccatgca
     1681 ccttcgcgtt cgcttttgac caacagtgcc gtgctttctt ccagttgatc gatcattttg
     1741 ttgtattcat gaatcaggga tcctatttcg tcctgctgat cccaggtcag ttgctcattc
     1801 tttttaccta attttatttt cctgagattg tccattagtc tgacgagggg cttggtcacc
     1861 gaattggcga caaaaaaggc aaggccgcct gctacaagaa gtaagaagac atacacattg
     1921 agtaaggtgc ccaggaagtc agaagcggca aatatagaag taacgcctgc ttccggatac
     1981 ggaaccgcaa gccagccgat tttttgatta tttgccttac ggacaggtac atatacggca
     2041 cggatagtat ttttttctcc ggcaaagtca aagtacacgg attgattatc gaaatataga
     2101 ttaagatagg gtaagggagg catcaggttt tgttttttac tcctaatctt tgcatatatg
     2161 gtgttaaaac gctcccttcc atcgatggcg taaaggctga agccggcttg ttgattggct
     2221 gagatcatgt ggagcgactt ctccggaatc gaatcaaggc cgccattccc taaggatgat
     2281 tctatgtctg atacaatgcc aaatgccttt tcgctcaaca ggctttcttc catcctcaat
     2341 gaaagattgt tgaaataact tccggtcaca aatgcgatga ttgcaaagga agctacaatg
     2401 accaggatga tgctgtattc tattcgctgg cgcagattga attgaccgga gatgaatatt
     2461 ggtagttctg ccggaagtat tctgaatctg ttgttgagga atgctacact gatgacgatt
     2521 gacaacagga tgacaattaa aaaagaacaa agtgagacag gttttaggat tcccatcagg
     2581 ttcttgcctg cgatgatgac ataaccgtct cccgccttcg atgcaatttc tgatcggttg
     2641 ttggaggtgg tttcgaccat attgccaccc ctggtaaaaa ctttctgtgt attcaggttg
     2701 tcagggtaaa tgtagccttg actctcaacc agattgtccc catgatagat ggcatagtta
     2761 tattttctca tcaggttcga gcccaggaat acggtgcggg gatccaaatc cctggtat
//
LOCUS       DAOQMC010000277         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_20769_length_2812_cov_5.900617, whole genome shotgun sequence.
ACCESSION   DAOQMC010000277 DAOQMC010000000
VERSION     DAOQMC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20769_length_2812_cov_5.900617"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PKV31_09350"
     CDS             complement(<1..647)
                     /locus_tag="PKV31_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNB93104.1"
                     /translation="MAVNILPLFKEQFESTLISKASELTGENFENSKSAINSIAVAFL
                     EKVTVKTSTQKGSLAVLSLIGQHCFDEMAALISNKDKEYDFNKIMNLGLILIKNIFAQ
                     KSGALIDWVSSDSGVKSSSAASIMSMVAPFIMCPIGQQVKSNGMNAAELTAMMTDQKP
                     FYASLLPSELAALINAPDPLTLERDVVKETKEEEQDLNLGKFLPWLLLILGLCLV"
     gene            1044..>2812
                     /locus_tag="PKV31_09355"
     CDS             1044..>2812
                     /locus_tag="PKV31_09355"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNB93105.1"
                     /translation="MVSLMVLGYGQPIKKVFIEEGSGTWCTNCPRGDVYVRELTKKYP
                     GQFVFVTVHLSDPMEIKEYADAVPFTGIPAGWFDRSSIYTLLPFTDLDSDMAAHLSKE
                     APAEVTVQTGWNADTREITIKVSAEMAGNLSGDFRLAAIVVEDGVTGTGPQYDQVNIY
                     SGGALGPMGGYENLPNPVPASRMVYNHVARHLAGGINGAENSLPADLNAGQSYSYVYK
                     YVLPEDYNEEYVKVAGLLIDANKGTVLNAGQSSYLNGSDNARPFFHSVPQESAFVGLD
                     YQYVIITHDPDFDSLTITQTGDLPEGISFKDEGNGTAQLYGKPTVTGTFPITLKLSDG
                     RHEIEQSFILEVSDAKEDWIQVGKPGFSTFEATSISLKINKEGTAYVLAADISGKNIV
                     VYSKDKNTEPWKQLGGVIKGDVFHSSMTLSDQGFPVVFSDKKVVGWNGTEWKAIGTDL
                     PGSACIYPAIVTAGDGTLYLVYFDIDKSTLTFKFDGSNWQPLGTVDKKYTVWNQFKTD
                     FAGRPMLIYGTEGVNISYSKVAILDNDQWVVQGGGYIEPASQTYFYHDAVASSTGNIY
                     AGLTIGAAEQGLNIYQLKNDKWE"
BASE COUNT          842 a          535 c          637 g          798 t
ORIGIN      
        1 caaacaaggc aaagaccaag aatcagcaaa agccaaggca ggaactttcc aagattcaag
       61 tcttgttcct cctccttcgt ttcttttacc acatctctct caagcgtcaa tggatccgga
      121 gcattgatca atgcagccaa ttctgaaggc aacaaggatg cataaaatgg tttctggtct
      181 gtcatcatgg ctgtgagctc ggcagcgttc atgccattgc tttttacttg ttgtcctatc
      241 ggacacatga tgaagggagc gaccatgctc atgatggagg cggccgatga tgatttaacc
      301 ccggaatccg atgagaccca atcaatcaaa gctcctgact tttgtgcgaa gatatttttg
      361 attagaataa gtcccagatt catgatttta ttaaaatcgt attctttgtc tttattcgag
      421 attagggcgg ccatctcatc aaaacaatgt tgaccaatca ggctcagcac agctaacgaa
      481 cccttttgtg tcgatgtttt caccgtgact ttttcaagga aagcgacagc aatggagttg
      541 attgcagact ttgaattttc aaaattttcc ccggttaatt cacttgcctt tgatataagg
      601 gttgactcaa actgctcttt aaaaagtggc aaaatattga ctgccatggt ttataacata
      661 ttaaaaatta gtcgtaaaag cacataatac atgcctcacc atcttattag atgtaaaaaa
      721 attaaaaagt cactcgtttg aagatgtttt tgtataattt ataatttgta aaaataacaa
      781 atgatcaggg attaaagaat taaaattcaa ttaaatgtga gtatctggaa atggcggcaa
      841 atattcatga ataaattggc aaggatacgg aaggctggcg gggtaataaa gtacgccgat
      901 gatttgagca actaaaaagg atataaaaat gcgtctaaat acatacttac cttatcggaa
      961 tcaattgata gaaaagctgt aatttggttt tttatttaca ggtaaccaaa atgatcatga
     1021 aactaatttt tacaatattt tcaatggtat ccttaatggt tttgggatac ggacaaccta
     1081 ttaagaaagt gtttattgag gagggaagtg gcacctggtg caccaattgc ccccggggag
     1141 atgtctatgt ccgggagttg acaaaaaaat atccgggtca atttgtattt gtgacggtgc
     1201 atttatcaga ccccatggag ataaaggagt atgctgatgc ggtgcccttt acaggaattc
     1261 ctgcaggttg gtttgaccgt tcttccattt atactttgtt gccctttacc gatttggatt
     1321 cagacatggc agcgcatctc agtaaggaag caccggctga ggtaacagtg cagaccgggt
     1381 ggaatgctga tacccgcgag attactatta aagtcagtgc cgaaatggcc gggaatctct
     1441 cgggtgattt tcgattggcg gctattgtgg ttgaagatgg agttacagga acaggaccac
     1501 aatatgacca ggtaaatatc tattcaggcg gagctttagg gcccatgggc ggatatgaaa
     1561 atctacccaa cccggttcca gcttcacgaa tggtatataa ccacgttgcc cgccatcttg
     1621 ccggcgggat caatggcgca gagaattccc ttcctgctga tctgaacgca gggcaatcat
     1681 attcatatgt ttataaatat gtgcttccag aagattacaa tgaagaatat gtcaaagtgg
     1741 ctggtttgtt gattgacgcc aataaaggga ccgtgttgaa tgcaggacaa agctcatatc
     1801 ttaatggcag tgataatgca cgcccctttt tccattctgt accccaggaa agcgcttttg
     1861 taggattgga ttatcaatat gtaattataa ctcacgaccc cgattttgat tctttgacca
     1921 tcacacagac gggagattta ccggaaggca tttcgtttaa ggatgagggt aatggaaccg
     1981 ctcaattgta tggtaagccg acagtaaccg gaacctttcc tattaccctt aaattatcgg
     2041 atggaaggca cgaaatagaa cagtccttta tattggaagt ttctgatgct aaggaagatt
     2101 ggatccaggt gggaaaaccg ggtttttcca cctttgaggc gacttccata agcttaaaaa
     2161 taaataaaga agggacggct tatgtactgg ccgcagacat ttcgggtaaa aatatcgtgg
     2221 tttattcaaa ggataaaaac acagaaccat ggaaacaact gggtggcgtg atcaaaggag
     2281 atgtgttcca cagttcgatg accttatccg accagggttt tccggttgtt ttttcagata
     2341 aaaaagttgt aggttggaat ggtactgaat ggaaagccat aggcacagac cttccgggca
     2401 gtgcctgtat ttatcctgcc atcgtgactg ccggagacgg aactttatac cttgtgtatt
     2461 ttgatatcga taaaagcacg ttaaccttta agtttgatgg ttcaaactgg cagcccttgg
     2521 gtacagttga taagaagtat acagtatgga atcaattcaa aacagacttt gctggcaggc
     2581 cgatgcttat ttacggaacc gaaggagtaa atatttccta ttcaaaggtg gctattttgg
     2641 acaacgatca atgggtagtt cagggtggcg gctatattga gcccgcttct caaacatatt
     2701 tttatcatga tgccgtcgcc tcatccaccg gaaacatata tgcgggattg actatagggg
     2761 cagctgaaca gggccttaac atctatcaac tgaaaaacga caaatgggaa tt
//
LOCUS       DAOQMC010000278         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_20959_length_2797_cov_5.121080, whole genome shotgun sequence.
ACCESSION   DAOQMC010000278 DAOQMC010000000
VERSION     DAOQMC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20959_length_2797_cov_5.121080"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..785)
                     /locus_tag="PKV31_09360"
     CDS             complement(231..785)
                     /locus_tag="PKV31_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNB93106.1"
                     /translation="MGRFAFFIESLKNIRTVGTVTRSSRFLCQSVVRHADLKKAHVVV
                     ELGAGDGVMTRHLLKAMSKDSYLISFEINKVFCERLSKINDSRLIIINDSAETLAQVI
                     ESRGLDKVDVVVSALPFSVFPEDLTKAIVGQCHALMRAGGRFVQIHYSLKTKEIYRNI
                     FGNVGSAFEVRNIPPAYVLYCFKT"
     gene            complement(833..>2797)
                     /locus_tag="PKV31_09365"
     CDS             complement(833..>2797)
                     /locus_tag="PKV31_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93107.1"
                     /translation="KNIYKNLGISLYYNNQLQEAESFLAEYYNIKPNDSEVVYYLGKV
                     FHHEMKFEKAIEFNKYYLSLTKPSGPRYQQVVADVKRCGNAKNIRYQEQEAISENSGR
                     EVNTTYDEFNPVLSPNFFGRVYYTSNQPVDTGRGSEVRLLPPSYNMFGADVQIGSGLM
                     GRGSLLNINLTTRQHEVLYGFNKDGRVLYFGRGDDLGQLSIMAENTNFDPEDSSRIYK
                     LDATFSKLGEARDVFIFSDSVLIFSSDMAGGVGGYDLYYSEYKNGKWGEPINLGDRVN
                     SPFDERSPFLAKDGRTLYFSSNNYNTIGGFDVFTTYYLDKNMEWSDPVNVGLPINSPG
                     DELYFKLTEDGLKAVFSSDRKSGFGGFDLYNAFFKSVRKEQLVEALPSVFFQVPDFKL
                     NSQEYRDEINQSKIVALTLEPMYYTDDASILQAKNKKQLDQLVELAERFPSVTFNFEI
                     NSESNVSSEIELYFGIKRGEIISNYMISRGVKGGRITIQSLGNLYPIARNIVDGKPSI
                     SGQNLNRRIEVRLSHLDSLPLKVTYKQPFVSDLLKAGDGARLRQRTNGLSYRVQFISL
                     RQMYTGGIYSLNPDLLIESLGGSGLYRYMSGLFDNYADAKAFKDILINRGLLDSFVVP
                     YIDNERYAPETLTEAMMNKYPDLRKYFLDL"
BASE COUNT          860 a          650 c          452 g          835 t
ORIGIN      
        1 gatgaaggtc ttagcaggca aattgatgtg attgaaattg aaaaaaataa ggattattaa
       61 tcaaacctac tcaaccaggg tttcttacct tctttttcct ttgtagcctg gaatatccaa
      121 caactccggc ctaattcaag ttgtgtaaag gaaatgctct ctctaattaa ttgataagaa
      181 tacgaaaaaa ccaatctttc aaattctttg ataaatctag gataaagcat ctatgtttta
      241 aaacagtata atacgtaagc cggtggtata ttccgtacct caaaagccga tcctacatta
      301 ccaaaaatat ttcggtaaat ctctttggtt tttaagctat agtgaatttg aacaaatcta
      361 cctcccgccc tcatcaaagc atgacactgg ccaactattg ccttggtaag atcttccgga
      421 aaaaccgaaa aaggcagggc cgataccacg acatctactt tatccaggcc tcgcgactca
      481 atcacctgag caagcgtctc agcagaatca ttgatgatga taagccggga atcatttatc
      541 ttacttagcc tttcacaaaa aaccttattt atttcaaagg atatcaaata cgaatctttt
      601 gacatcgcct taagcaagtg tcttgtcatc actccatccc ctgcaccaag ttcaacaaca
      661 acatgagctt ttttcaggtc tgcatgtctg acaaccgact gacacaaaaa acgtgaactc
      721 cgagtgacag tccctacagt cctgatattt ttcagacttt caataaaaaa agcaaaacgg
      781 cccatatgtc ggtatttccc ttcgggttta tctattgttt gagttatctc cattaaagat
      841 caagaaaata ttttcgcaaa tcgggatact tattcatcat tgcctcagtc aatgtttcag
      901 gggcataacg ctcattatca atatagggta caacgaatga gtctaataaa ccacggttta
      961 tgagtatgtc cttgaatgcc tttgcatcgg catagttatc aaataagccg gacatatacc
     1021 tgtacaatcc actaccacca agactttcta taagtaaatc aggattgagg gaataaattc
     1081 cccccgtgta catctgacgc aatgaaataa attgtaccct gtatgaaagc ccattggttc
     1141 gctgccgcaa tcttgcacca tcacctgctt ttaacaagtc actgacgaaa ggttgcttat
     1201 aggtgacttt caaaggcaat gaatccaaat gactcaaccg cacttcaatt cttctgttga
     1261 ggttttgacc tgaaatactc ggcttcccat ctactatatt tctggctatc ggatacagat
     1321 tgcccagact ttgaattgta atcctgcctc ctttaacacc tcttgaaatc atataattac
     1381 tgatgatttc tccacgctta ataccgaaat aaagttctat ttcggaagaa acattcgatt
     1441 cgctattgat ttcaaaatta aatgtaacag agggaaatct ttcagccaat tctaccagct
     1501 gatctaactg ttttttattc ttggcctgca ggattgatgc atcgtccgta taatacatgg
     1561 gttcgagggt taatgcaaca atcttgcttt gattgatctc gtcccgatac tcctgactat
     1621 tcaatttaaa atcgggcact tgaaaaaaga ccgaaggaag tgcctctacc aactgttcct
     1681 tcctgaccga tttaaaaaag gcattataca agtcaaatcc tccgaatccc gattttcgat
     1741 cagaactaaa taccgctttc aaaccatcct cagtcaactt gaaatacaac tcatctcccg
     1801 gggagtttat ggggagaccc acattgacag ggtcgctcca ttccatattt ttatccaggt
     1861 agtaggtagt aaatacatca aaacctccta ttgtattata attattactc gaaaaataca
     1921 aggtccggcc atctttggcc aagaaaggac ttctttcatc aaatggagaa ttcaccctgt
     1981 ctcccaaatt gattggttca ccccatttgc catttttata ctctgaataa tacaaatcat
     2041 agcctccaac ccccccggcc atgtcagaac tgaatatcag aactgaatca gaaaaaataa
     2101 atacatccct tgcctctcct aacttcgaaa atgttgcatc caacttatag atccgactcg
     2161 aatcctcagg gtcaaaattg gtattttccg ccattatact aagctgcccc aaatcatcgc
     2221 ccctgccaaa atacaacaca cgaccatcct tattaaaacc atacaatacc tcatgttgtc
     2281 gggtagtgag atttatattc aaaaggctcc ctcttcccat taggccactt ccaatctgaa
     2341 catctgctcc aaacatatta taacttgggg gcagcaagcg cacttcacta cctctacccg
     2401 tatctacagg ctgattggat gtataataga cccgcccgaa aaaattggga cttagcacag
     2461 gattgaattc atcataggtt gtatttactt cgcgccctga attctcactg atagcttcct
     2521 gttcctgata tcttatattc tttgcattcc cacaccgctt cacatcggca accacctgct
     2581 gataccgggg accggatggc ttagttaggg acagataata tttattaaat tcaatggctt
     2641 tttcaaattt catttcatga tgaaaaacct ttccaaggta atatacgacc tcgctatcat
     2701 ttggcttaat gttgtaatat tctgccaaaa aggattcagc ttcttgtaat tgattattgt
     2761 aatacaagga aatgcccagg tttttgtaaa tattctt
//
LOCUS       DAOQMC010000279         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_21400_length_2768_cov_5.739034, whole genome shotgun sequence.
ACCESSION   DAOQMC010000279 DAOQMC010000000
VERSION     DAOQMC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21400_length_2768_cov_5.739034"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..2449)
                     /locus_tag="PKV31_09370"
     CDS             complement(101..2449)
                     /locus_tag="PKV31_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB93108.1"
                     /translation="MRHILLVFFILIIGSSTRAQQLNIYDWASLQFYRSGTDVTETPN
                     NIVFNAGSGLVFINKSNIDDIRHVTTIDGLSGSNIINVRYIPDAKKLFIYYSDGSFDL
                     MDESLGITSVQDIKNNNVLVPNKALNAAVVDGKYIYLCFSFGMVEYDATEEVFTNTLS
                     AEGGIGWITVNGNRFYIINNQRLYTIDRSVKPNISFIGNWTLLDAPDSLTHNDASYRG
                     LTTFLGKVYTTFDRKLYRLEEDDSLTRIDSLPTVKKVAYLNAGETYLMVGGSCNSAVL
                     DNKSCIGEVALFSQNLDPIDYSRLNLADLRYAIETKDKIWFADSYHGFRYLDLPVLTP
                     GLIDINRISPWSDKVDAIIKTADALYIAPGAHTSIYQGAGNKEGLFEYKDGFWWVYNQ
                     FSDSFFASPRIISDLSNFAFNENTGELAIASYTDGIISIKGNEKKLYYSENSALQTVP
                     GDPNNTRVSDVGFDSKGNLWATNVLSPTSIYVRTESGVDKGFANNFGQIRLIDMSIDP
                     INEYLWCVEPSRGIVVYDPGKEVLSSNDDRWANFQVYSSFENQNILPKCVYADNEGRI
                     WIGTVSGIFVEDCGSGVFDGSENCGTRFPIMQDETGLGPFLYGQSVNFITEDGAGRKW
                     IGTSNGIYVTNNAVDEVVLHLTTDNSPLADNNVTVLEIDKKAGIVYIGTRAGVQMYRT
                     DASIGNPYHDAKLLIYPNPVRPEYSGPIIIQGLAHDSDVKIADINGKLVYETHSLGGQ
                     AIWYGKDFNGRKVGSGVYFVIGTTTQNTENPAGIIGKVVLVH"
BASE COUNT          785 a          638 c          579 g          766 t
ORIGIN      
        1 cggaaacttc caatctgcgc agaatttcat ccgcataagc gcaacttggg ttaagtgagc
       61 gctatcgcat ttattggttg cgtcaggaga tcaaagcctt tcaatgcacc aacaccacct
      121 taccaatgat accggctgga ttttctgtat tctgagtcgt tgtgccgatg acgaaataaa
      181 cgcccgagcc taccttgcgc ccattgaaat ccttgccata ccatattgcc tgacctccca
      241 gtgaatgcgt ctcatacacc agcttaccat tgatatcagc aatcttaaca tccgagtcat
      301 gagccaaccc ttgtatgatt atggggccac tatactccgg cctgaccgga ttgggataga
      361 tcaacaactt ggcatcatgg tatggattac cgattgaagc atccgtgcga tacatctgca
      421 ctccggcacg cgtgccgata tatactatgc ctgccttctt atcgatttca agcaccgtga
      481 cattgttgtc cgccagtggc gaattgtctg tggtcaaatg cagaaccact tcatctacgg
      541 cattgttggt cacatatatg ccattgctgg tgcctatcca ttttctaccg gcaccatctt
      601 cagtaatgaa attgaccgat tggccataaa ggaaggggcc aagccctgtt tcatcctgca
      661 tgatcggaaa ccttgtccca caattttcac taccatcaaa cacaccagag ccgcaatcct
      721 ctacgaagat acccgaaact gttccaatcc atattctgcc ctcattgtcc gcatagacac
      781 attttggtaa aatattttga ttctcaaaac tggaatatac ctggaaattg gcccatcggt
      841 catcgtttga actcagtact tccttgcccg gatcgtaaac gactatgcct cgtgaaggct
      901 ccacacacca tagatattcg ttgatgggat cgatggacat atctatgagt cggatttgac
      961 cgaagttatt ggcaaagccc ttgtcgacac cgctttcagt tcttacatag attgatgtcg
     1021 ggctgagtac atttgtcgcc caaagattgc cctttgagtc gaagcccaca tcacttacac
     1081 gagtattgtt cgggtctccc ggcactgtct gcaaagctga gttttcagaa taatacaatt
     1141 ttttttcatt gcccttgatg gaaatgatac catcagtata agaggcaata gccaactccc
     1201 cggtattttc attaaaggca aaattggata agtcggaaat aatgcgcgga gaagcaaaaa
     1261 atgagtcaga gaattgattg taaacccacc agaatccatc cttatattca aacaatcctt
     1321 ccttgttgcc cgcaccctgg tatatcgaag tatgggcgcc aggcgcgata taaagtgcat
     1381 cagccgtctt gatgatggca tcaaccttgt ccgaccaggg tgatatccga ttgatatcga
     1441 taagcccggg tgtcaagaca ggcaggtcaa gatatcgaaa accgtgataa ctatcggcaa
     1501 accaaatctt atccttagtt tctattgcgt accggagatc agccaggttg agtctgctat
     1561 aatctatggg atcaagattc tgactaaaca aggccacttc cccaatacaa cttttattgt
     1621 ccagcactgc cgaattgcac gatccgccaa ccattagata agtctcgcca gcattgaggt
     1681 aagcaacctt cttgactgta ggtaaagaat caatgcgggt gagggaatca tcctcctcca
     1741 gacggtaaag ctttcggtca aaagtggtat aaacctttcc gagaaaggtg gtgaggcctc
     1801 tgtacgaagc atcattatga gtgagactat ccggtgcatc cagcagagtc cagttaccta
     1861 taaaagatat attgggcttg actgagcgat cgatggtata caatcgctgg ttattaatga
     1921 tgtaaaaccg gttaccattg acagtgatcc agccgattcc tccttctgct gagagcgtgt
     1981 tggtaaatac ctcttcggtg gcatcgtatt ccaccattcc gaaggaaaaa cacagataga
     2041 tgtacttgcc atctacgacg gcagcgttca atgccttatt aggcaccagc acattattgt
     2101 tcttgatatc ctgcactgaa gtgattccca gactctcatc cattaggtca aaactaccat
     2161 cgctatagta gatgaatagt ttctttgcat ccggaatata tctcacattg atgatatttg
     2221 aaccactcag gccatcgatg gttgtgacat gtcggatatc atcgatgttt gatttgttaa
     2281 taaataccag accggaaccg gcattgaata caatattatt tggtgtctcg gtaacatcgg
     2341 tgccggaacg ataaaactgg agactggccc agtcgtatat gttcagctgc tgtgccctcg
     2401 tcgaagatcc tatgattagg ataaagaata caagtaaaat gtgtcgcata atattcaaga
     2461 tgccaatttc tccaatattg tccaataaaa gcgtaccgag ggcattccaa attaatcgcc
     2521 ggcgggattg ctctattttc cagaattgat ggaggcaaag gtaaaaacag ttcggtctgt
     2581 ttcgccatta tttctcctga cagatagaac gaaataaatt tttataaaat tttgaatgat
     2641 aaaaataata tcttgaggcg tattagaatg agttgatttg tggaataaaa aaattattat
     2701 gcaaacaagg cgtcaatttg taaaagatgt ggcaaaagca tctgcagtgg ttgctgctcc
     2761 gaatttta
//
LOCUS       DAOQMC010000280         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_21468_length_2763_cov_4.600812, whole genome shotgun sequence.
ACCESSION   DAOQMC010000280 DAOQMC010000000
VERSION     DAOQMC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21468_length_2763_cov_4.600812"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKV31_09375"
     CDS             complement(<1..356)
                     /locus_tag="PKV31_09375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB93109.1"
                     /translation="MSWTIAFILILFAIYAFSIFLITIEWLKTRILNTNPELDGISFS
                     IIIPFRNEEKNIEQCIKSIVDQQYDPGKFEIICVDDQSSDRSAEIISTFAAKYKMIRL
                     IDSNGNGKKAALQTGI"
     gene            complement(385..921)
                     /locus_tag="PKV31_09380"
     CDS             complement(385..921)
                     /locus_tag="PKV31_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNB93110.1"
                     /translation="MEIKKVQKNNSARKNISEDKTVEIPLNDKLFSEVKMIFSEFLEK
                     NQCRKTPERYAILEEIYRRGDHFDAETLYFHMKNHNYRVSRATVYNTLELLVNCDLVK
                     KHQFGKNLAQYEKSYGYKQHDHLICEKCGKVVEFCDPRIQNIKNMIGEMLNFNITHHS
                     LNLYGTCLGGCEAIQSEE"
     gene            complement(951..>2763)
                     /locus_tag="PKV31_09385"
     CDS             complement(951..>2763)
                     /locus_tag="PKV31_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNB93111.1"
                     /translation="LSTLNSDVRVVLIKMADRLHNMRTLGSMKRDKQLKIASETSYIY
                     APLAHRLGLYKLKTEFMDLCLKILEPSLYQEIARKLQETKRSRNHYVNEFIKGMHDQL
                     EELGVPYRITGRPKSISSIAEKLKTKGVPFEEIYDLFAVRIIVDVEPKREKSVCWQVY
                     SVITDVYTPVPERLKDWVTMPKSNGYESLHTTVVGPGGRYVEVQIRSERMDEIAEKGF
                     AAHWKYKGVKQSSQNVFDAWLDSVRTLLEDKDKDALEFISEFKSNLSGEDVYVYTPKG
                     DMVTLPKGATALDFAFNIHSDVGYHTISIKINNKLVPMGYVLENGDQIQVITNKNQKP
                     NESWLKMVITGKARNKIKQALKEEDKAQAEYGKEELERKLSNQKLEFDENVDFLVKHF
                     GLKTRFELYLGISKHQIKIDLKKFKVEGNKLMPPPVVTKIKTEETSDELGNVSYAIEP
                     GKNKSFLLINGESADKFSYQFATCCHPLPGDDIFAFVTTANTLKIHRASCPNSEHLLA
                     SYGYRVLKAEWANFVSSSFLEELVITGTDKGQGVILAITETINKFGLNIKSFTIAGNE
                     GVFEGLVKLEIRDLDQLNALIKALKNIPYISNVRRSN"
BASE COUNT          731 a          577 c          510 g          945 t
ORIGIN      
        1 tcgatgcccg tttggagtgc agctttctta ccgttgccat tactgtcaat cagcctgatc
       61 attttgtatt tggctgcgaa tgtgctgatt atttctgcag agcgatcact agattgatca
      121 tcaacacaga ttatttcaaa tttacccgga tcatattgct ggtctactat acttttgatg
      181 cactgttcaa tatttttttc ttcatttctg aatggtatga taatcgaaaa acttatcccg
      241 tccaactcag ggttggtgtt cagaatcctg gtcttcaacc attcgatggt gataagaaag
      301 atactgaaag catatatagc aaacagaatc agaataaatg ctattgtcca ggacatttag
      361 tattgtattt atctcccggg agaattattc ttcagactga attgcttcac atccacctaa
      421 acatgtgcca tataaattga gagaatggtg tgtaatatta aaattgagca tttcgcctat
      481 catattcttg atattctgga tgcgagggtc acagaactca actacttttc cacatttttc
      541 acatatcaaa tggtcgtgtt gtttgtaacc atatgatttt tcatattgtg caaggttctt
      601 gccgaattgg tgttttttga ccagatcaca attgacaagc aattcgagag tattgtatac
      661 cgtagctctt gaaaccctgt agttgtgatt tttcatatgg aaatacaatg tctcggcatc
      721 aaaatgatcc cctcttcgat atatctcttc aagtattgca tacctttcag gagtttttcg
      781 acattgattt ttctccagaa attccgagaa tatcatctta acttcagaaa acagtttatc
      841 attcaaagga atttcaactg ttttgtcctc tgaaatattt ttcctcgcac tattgttctt
      901 ttgtactttt ttaatttcca tatttttaat ttacgagaaa gctttgtatg tcaattactt
      961 cttctaacat ttgaaatata aggaatgttt ttcaatgcct taattaaggc attcaattga
     1021 tccaaatccc tgatctcaag tttcacaaga ccttcgaaca ctccttcatt gcctgctatt
     1081 gtgaaagatt tgatattgag accaaatttg ttgattgtct ctgtaatggc aaggataaca
     1141 ccctgacctt tgtctgttcc tgttattacc aattcttcca ggaaagaaga agaaacgaaa
     1201 ttagcccatt cggcctttaa cacccgatat ccataggagg caagtagatg ctcactgttt
     1261 gggcatgatg cccggtgaat cttcagcgta ttggctgtgg tgacgaaagc aaaaatgtcg
     1321 tcaccgggga gggggtgaca gcaggtggca aactggtaac taaatttatc agcgctttcc
     1381 ccattgatga gaagaaacga tttattcttg ccgggttcaa tggcatagga tacatttcca
     1441 agttcatcac tggtctcttc tgttttgatt ttagtcacta caggaggtgg catcaactta
     1501 ttaccttcaa ccttaaattt ctttaagtca atcttaattt gatgctttga aatgccaagg
     1561 tatagttcaa atctggtttt caaaccgaaa tgcttgacca ggaagtcaac attttcatca
     1621 aattctaact tttgattgga aagtttcctt tccagctctt ccttgccata ttctgcctgg
     1681 gctttatctt cttccttgag tgcctgtttt atcttattgc gggccttgcc ggtaatcacc
     1741 attttcaacc aactttcatt tggtttttgg tttttgttgg taatgacctg aatctgatca
     1801 ccattttcca ggacgtatcc catcggtaca agcttgttgt tgatctttat actgatggtg
     1861 tggtagccca catcggagtg tatattgaag gcaaaatcca aggcagttgc acctttgggc
     1921 agtgttacca tatcaccttt aggtgtatag acatatacat cttctccact caggttggat
     1981 ttgaattcag aaataaattc caatgcatcc ttgtctttat cttccaacaa ggttcggact
     2041 gaatcaagcc atgcatcgaa tacattctgg ctactctgct tcactccctt atatttccag
     2101 tgggctgcaa atcctttttc ggctatttca tccatccttt cagacctaat ctgcacctct
     2161 acgtatcgac cgccagggcc taccacagta gtgtgcaatg attcataacc gtttgacttt
     2221 ggcatggtta cccaatcttt aagacgttcg gggacaggtg tatagacatc tgtaattaca
     2281 gaatacactt gccagcaaac acttttctcc cttttgggct caacatccac gatgattctg
     2341 actgcaaaga gatcatatat ttcctcaaag ggtactccct tagtctttag cttttcggca
     2401 atggatgaga tagatttggg tcgacctgtg atcctgtatg gtactccgag ttcctccagt
     2461 tgatcatgca tgcctttgat aaattcattg acataatgat tcctgcttct cttagtctct
     2521 tgaagtttcc ttgcaatctc ctgatataaa gagggctcga gaatctttag acaaaggtcc
     2581 atgaattcag tcttaagctt atacaagcca agacggtggg ctaggggagc atagatgtag
     2641 cttgtttcag aagcaatttt taattgtttg tcgcgcttca tggagcctaa ggtccgcata
     2701 ttatgcagcc tgtcagccat tttaatcaac acgacccgga catctgaatt cagtgtactc
     2761 aat
//
LOCUS       DAOQMC010000281         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_21535_length_2758_cov_3.960414, whole genome shotgun sequence.
ACCESSION   DAOQMC010000281 DAOQMC010000000
VERSION     DAOQMC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21535_length_2758_cov_3.960414"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="ychF"
                     /locus_tag="PKV31_09390"
     CDS             complement(<1..898)
                     /gene="ychF"
                     /locus_tag="PKV31_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNB93112.1"
                     /translation="MALKCGIVGLPNVGKSTLFNALTSAKALAANYPFATKEPNLGVI
                     TVPDKRLDQLSDIVRPQKVQPTTVEILDIAGLIKGASKGEGLGNQFLANIREVDAIIH
                     VIRCFQDDNVVHVDGSINPVRDKDVIDTELQLKDIETLEKRLDKIQKAAKTGDKAAKA
                     TFDLGQRMLAHLGDGNNARSLDLTEEEALEIKEFQLLTGKPILYVCNVDEDSAVSGNE
                     YSAAIKELVAAENAQVVIISAGIEADIAELETKEERLEFLEAIGLSEPGVNKIISAAY
                     RLLGLITYFTAGEKEVRAWTITE"
     gene            complement(1145..1996)
                     /locus_tag="PKV31_09395"
     CDS             complement(1145..1996)
                     /locus_tag="PKV31_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNB93113.1"
                     /translation="MSIAASELILNEDGSIYHLGLHPEQVYPTIITVGDPDRVEKVSK
                     YFDRIDFKKQKREFISHGGRIGNRDITVVSTGIGTDNVDIVINELDALVNIDLDTRTP
                     KSDIRKLDIMRLGTSGTIRAEVEVDSLVLSRGAIGMDGLMHFYKRDKKPEELEMLTWE
                     INWPLPPYYYSGSESLLARYTYLGIEGITVSNTGFYGPQSRRLRLDPASDNLFETLAG
                     QSINGCPITNLEMETSGIYGLCQLLGHNCLSINVIIANRSLGKFSNAPDKAVINMIET
                     ILEEASK"
     gene            complement(1987..2466)
                     /locus_tag="PKV31_09400"
     CDS             complement(1987..2466)
                     /locus_tag="PKV31_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93114.1"
                     /translation="MMKTINLIIFLLLIQWSCLKAPEYPIEPIISFISVSNSVIKQSQ
                     FNGDSTYITFGFTDGDGDLGDQDSVNIFVTDQRTNIHPTVYKIPFLKQNNGAKGISGE
                     IRIRLFSECCIYPNGQVPCTPSTEFPTDTLIYSIYIKDRAGHQSNIIYTTPIVLLCQ"
BASE COUNT          728 a          565 c          560 g          905 t
ORIGIN      
        1 cttctgttat tgtccaggct ctgacttctt tttcaccggc tgtaaaatat gtaatcagtc
       61 cgaggagtct ataggcagct gatatgattt tgttgacacc cggctcagac aggcctatag
      121 cctctaagaa ctccagtctt tcctctttag tctccaattc agcaatatca gcctctatcc
      181 ccgctgaaat gataactacc tgtgcatttt cagctgccac gagttccttg attgcagcag
      241 aatattcatt gccggagact gcagaatctt catctacatt gcaaacatat aaaatgggct
      301 ttccggtcag gagctggaat tcctttattt ccaatgcctc ttcttcagtc agatccagtg
      361 atcgggcatt attgccatct cctaagtgag cgagcattct ttggcccaaa tcaaatgtgg
      421 ctttggctgc cttatctccc gttttagccg ctttttgaat tttatccagt cttttttcca
      481 gtgtttcgat atccttaagt tggagttcag tatctatgac gtccttatcc cgaactggat
      541 tgatggaacc atcgacatgc acgacattgt catcctgaaa gcatcgtatg acatgaatga
      601 tggcatcgac ttccctgata ttggccagga attgattgcc gagtccctca cctttgctgg
      661 ctccttttat tagccctgcg atatcgagga tttcgacggt ggtcggttgc actttttgag
      721 gccggacaat atccgacaat tgatccaatc gtttatcagg aacggtgatt acaccgagat
      781 taggttcttt ggtggcaaag ggataatttg ctgcaagggc ttttgctgag gttagggcat
      841 tgaagagggt cgatttgcct acattaggta gtccgacgat accgcatttg agagccatat
      901 tttattttgt ttttggccgc gaagatacat ttaatttcag gcataaaact gtctttgtca
      961 aaaatctttt atttagcgat atctatgagc gatgcttata tatcttcaag aagcttcttt
     1021 atcgggagga ttgcatattt ccgggctgaa agagaaaaag ggcgatccga ttgattttaa
     1081 gggtacttga tacaaaggta ataaaagtaa tattgagcat caatatcatt aaataacgta
     1141 taatttattt acttgcttct tccaatattg tctcgatcat attgatcacc gccttgtcgg
     1201 gtgcattact gaatttacct aaggatctat tcgctatgat tacattgatg gacaggcaat
     1261 tatggcctag aagttgacaa agtccgtata tgcctgatgt ctccatctca agattagtta
     1321 tcgggcatcc gttaatactt tgaccggcaa gggtttcaaa taagttgtcc gatgcagggt
     1381 caagtcgcaa ccttcggctt tgaggaccgt aaaagccggt attagatacg gttatccctt
     1441 caataccgag atatgtgtac cttgccagca aactttcaga cccggaatag taataaggtg
     1501 gcaaaggcca gttaatttcc caggttaaca tttccagttc ttcaggcttt ttatctcttt
     1561 tgtaaaaatg catcaggccg tccatcccta ttgctccgcg agacaggacc agactgtcca
     1621 cttcgacctc tgctctaata gttccggatg taccaagcct catgatatcc agtttcctga
     1681 tatctgattt tggtgtcctc gtatccaaat cgatattgac taaggcatcc aattcattga
     1741 tgacgatatc aacattgtct gttcctattc cagttgagac tacagtaatg tccctgttac
     1801 ctattcggcc tccatgcgag atgaattctc ttttttgttt tttaaaatca atccggtcga
     1861 agtatttgct tactttttct acccgatcag gatctccgac agtaatgatg gtcgggtata
     1921 cctgttccgg gtgtaatccg agatgataaa tacttccgtc ttcattgaga atcagttctg
     1981 aagctgctat tgacataata atacgattgg cgttgtgtaa atgatgttgc tttggtggcc
     2041 ggctctatct ttaatataaa tactatatat cagggtatcg gttggaaatt ctgttgaagg
     2101 tgtgcatggg acttgtccgt tagggtaaat acagcattca gagaataacc ttattctaat
     2161 ttctcctgat atacctttgg caccattgtt ctgttttaaa aagggaatct tgtacacggt
     2221 tggatgtata tttgtccgtt ggtccgtcac gaagatgttg acggaatcct ggtccccaag
     2281 atcaccatct ccatccgtaa atccaaaagt gatataagtg ctgtctccat tgaactgact
     2341 ttgtttgatt acggagttgc tgactgaaat gaaactgatg attggctcaa tgggatattc
     2401 cggtgctttc agacaagacc actgaatcaa aagaagaaat attatgaggt tgatggtttt
     2461 catcatatat aattaaatac atccaacggc gtaaaattta caacaaattt gcttcaccag
     2521 ataatccaat aaattttccc tgtgattggg gttaaatata catatttgca ttgtaaaccg
     2581 aaattctttt tgcttcatgg ccgataatat tctcccggaa gagattctaa aactcagccg
     2641 ccaactggta atgggtgaaa taatattggt caatgaagta tatcctgtca tatttgattt
     2701 tcagtcgaaa tttaatccgg tatatcgctc ttatttaaaa cagttgaagt ttgatttg
//
LOCUS       DAOQMC010000282         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_21837_length_2737_cov_5.738628, whole genome shotgun sequence.
ACCESSION   DAOQMC010000282 DAOQMC010000000
VERSION     DAOQMC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21837_length_2737_cov_5.738628"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..2245
                     /locus_tag="PKV31_09405"
     CDS             68..2245
                     /locus_tag="PKV31_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB93115.1"
                     /translation="MKKLFILFLAISSSALLIAQGQKSFDLNDVFRKGTFRTKSLPGF
                     AYMKDGKSYIINKGDALEINHVLDGKSLGTYISSEQWIALAGDNSAGIESYQFSEDES
                     KVLIVTGSEQIFRHSSKGKTFVFDKKKGSGVVVFDGKPVSNPTFSPDGMKVGFTCENN
                     LYYMDLTTNTSTQVTNDGKWNFIINGMCDWVYEEEFSFTRAYEWSSDSRKIGFLRFDE
                     SKVPEYSMEYYRDEMYPIPFSFKYPKVGEDNSTLEVKIYNLDTRNTVTIDQIPDKEIY
                     YPRIKWTRDPNKLCVFKMNRHQNNLELLLADASSGKTTILMKEDNKYYIEINDYLTFL
                     KDGKHFIWVTDKGGFYQAFLHDMNGNRINAITQANYDLSNIYGYDENTKTLYYQLIAP
                     TPMQCEVYKVGLDGKKPVKLSIKDGNNAAQFSPTFEYMTLIYSNINTPTSWTLYDKKN
                     KIVRVLEDNQAAIDKFKEFKAGKAEFFSFTTSEGVQLNGYQIKPSNFDPNKKYPVFMY
                     LYGGPGSQQVVDRWGGGQSWWFKMIAESGYIVACVDNRGTGGRGEEFRKMTYMQLGHY
                     EVIDQIEAAKYLGSLPYVDAGRIGIFGWSYGGYMSSLCLLKGNDVFKAALAVAPVTNW
                     KWYDSIYTERYMRTYKENPDGFKDNSPIYFADRLKGNYFLAHGIADDNVHFQNSVEMN
                     RALIKAGKQFEFHMYPNSNHGIYSEGATMHLYNAMTKFIMDKI"
     gene            2403..>2737
                     /locus_tag="PKV31_09410"
     CDS             2403..>2737
                     /locus_tag="PKV31_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB93116.1"
                     /translation="MYVVPFTIPKLDTRSFHFQEDILPHFYRHLHRHQEVQMTLILKG
                     SGTLVAENRMHVFTKGDFFLLGSGQSHLFREDEDALNSLTKSGIHTLNLFFAPQGIIR
                     PLFDLPEMTV"
BASE COUNT          812 a          530 c          621 g          774 t
ORIGIN      
        1 gatatttttt tttatcttac gttcgatttt ctatttttaa cattgataat caaaattgac
       61 tgtgcaagtg aaaaaattat ttattttatt tctggctatc tcttcttctg ccttgttgat
      121 cgctcaaggt cagaaatcat tcgaccttaa tgatgttttc aggaaaggca cattccggac
      181 taaaagcttg ccgggatttg cctatatgaa agacggcaaa tcctatatca tcaataaggg
      241 agatgcattg gaaatcaatc atgtcctaga cggaaagagt ttgggcactt acattagcag
      301 cgaacaatgg attgccttgg ccggagataa cagtgccggt atcgagtcct atcagttcag
      361 cgaagatgaa agtaaagtgt tgattgttac gggctctgag caaattttca gacacagttc
      421 caaaggaaag acctttgtct ttgacaagaa aaagggcagt ggagttgttg tgtttgacgg
      481 caaaccggtc agcaatccga ctttttcacc ggatggaatg aaggtaggct ttacctgtga
      541 aaacaatttg tattatatgg atctgaccac taatacttcc actcaggtaa cgaatgatgg
      601 taaatggaat tttattatca acggtatgtg tgactgggtt tatgaagaag aattcagttt
      661 tacaagggct tacgaatggt cttcggacag tcggaagatt ggcttccttc gctttgacga
      721 gtctaaggtg cctgagtatt ctatggagta ttaccgggat gagatgtatc ctattccttt
      781 ttctttcaaa tatccgaagg tgggggagga taattccacc ctggaggtca agatatacaa
      841 tctggacacc cgcaatactg tcaccatcga ccagatccct gacaaggaga tatattatcc
      901 gcgcatcaaa tggacacgtg atccgaataa actgtgcgta ttcaagatga atcgccatca
      961 gaacaatctc gagctgcttc tcgccgatgc cagcagcggt aagacaacta tcctgatgaa
     1021 ggaagacaat aagtattata ttgagatcaa tgactatctg accttcctca aggatgggaa
     1081 gcacttcatc tgggtcaccg ataaaggcgg attctatcag gcctttctac acgacatgaa
     1141 tggcaaccga atcaatgcca tcacacaggc caattatgac ctttccaaca tatacggcta
     1201 tgatgaaaat accaagacct tgtattatca gttaatcgca cccactccca tgcagtgcga
     1261 agtgtacaag gtaggactgg acgggaagaa gccagtaaag ctatctatca aggatggtaa
     1321 caatgctgct cagttttctc ctacatttga atatatgaca ctcatttatt ccaatatcaa
     1381 tacacctacc agctggactt tgtatgataa aaagaacaag atagtccggg tattggaaga
     1441 taatcaggcg gcaatcgaca aattcaagga gtttaaggca ggaaaggcgg aattcttctc
     1501 tttcaccaca agtgaaggag tgcaacttaa tggataccag attaagccaa gtaattttga
     1561 tccgaataag aagtatcctg tatttatgta tctttatgga gggccgggaa gccagcaggt
     1621 agtggatcga tggggaggtg gtcagagttg gtggtttaaa atgattgcgg agagcggata
     1681 tattgttgca tgtgttgaca atcgaggtac cggaggtcgt ggagaagagt tccgtaaaat
     1741 gacctatatg cagttgggac attatgaagt gatcgaccag atagaagctg cgaaatacct
     1801 tgggtcatta ccatatgttg atgccggtcg catcggaatt tttggctggt cttatggcgg
     1861 atacatgtca tcactctgcc tgttgaaagg caacgacgtg ttcaaagcag cattggcagt
     1921 ggcaccggtc accaactgga aatggtatga ttctatttat accgaacgtt atatgcgtac
     1981 ttataaagaa aatccggatg gtttcaagga taacagcccg atttatttcg ccgataggct
     2041 caagggcaat tactttctgg cacatggcat cgctgatgat aacgtacact tccagaacag
     2101 tgtagagatg aaccgtgcct tgatcaaggc cgggaagcag tttgaattcc atatgtatcc
     2161 caacagcaac cacggcatat atagcgaagg agctaccatg catctgtata atgccatgac
     2221 taaatttatc atggacaaaa tttgacatct gcccgtttat ttagttgtgt gattttgtgc
     2281 agtatgattt catttattaa tgggcttaat gcaatttcag aaactgaaca gccccaaatg
     2341 actataaacg aattttaact acacaatata agatattgta tttttgtggt taaaattcgt
     2401 ttatgtacgt cgtccccttt actataccga agctggacac gaggagtttt cactttcagg
     2461 aagatatatt gccacatttc tacagacacc tgcatcgaca tcaggaagta cagatgacat
     2521 tgatcctcaa aggtagcggg accttggtag cggaaaacag gatgcatgtt tttactaaag
     2581 gtgacttctt tctgttgggt tccggtcaat cgcacttgtt cagagaagat gaagatgcct
     2641 tgaattcgtt gaccaaaagc gggatccata ccctgaatct attttttgca ccgcagggga
     2701 tcatcagacc tttatttgac ctgcccgaaa tgacagt
//
LOCUS       DAOQMC010000283         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_21840_length_2737_cov_5.206189, whole genome shotgun sequence.
ACCESSION   DAOQMC010000283 DAOQMC010000000
VERSION     DAOQMC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21840_length_2737_cov_5.206189"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKV31_09415"
     CDS             <1..683
                     /locus_tag="PKV31_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93117.1"
                     /translation="RLRPFIGLSAGIGLPFRKMVNHSVESELYIGRQGTEHPLYLTNI
                     QADAGLLLDDSWSIKSGIEWQQAVEQFSYKKFDATRLEYKTDDITGRIDDTSLVRGLL
                     TEQFGNKYTMLNIPLYLGYEKRFGRWVFGIDGGVGFNIHFRTSGKVLVDDQKIQSLDN
                     RHDIYASSTGISWRVGASCLRSIAPGTMVYARLMCMAYPDSWTLSTHPNEIRYRFLTA
                     NVGLRKVF"
     gene            complement(1214..1684)
                     /locus_tag="PKV31_09420"
     CDS             complement(1214..1684)
                     /locus_tag="PKV31_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNB93118.1"
                     /translation="MALEIERKFLVDHDAWEKVDRPVGVPFRQGYIVKDQQKVVRVRI
                     TPDSGFITIKGESKGAVRSEFEYPIPREDANELLELFCSYEVAKRRYFMSFRAKTWEV
                     DVFEGKNSGLIVAEIELNSADELFEKPDWVGEEVTEDPRYYNAFLAEHPYAEWM"
     gene            1817..2392
                     /locus_tag="PKV31_09425"
     CDS             1817..2392
                     /locus_tag="PKV31_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB93119.1"
                     /translation="MEILIRRACPEDNEHIVAHIILAMEDIIFRFIGEESSEKAFAFL
                     SSLVKERGNLYSYENCWVAEHGSEVVATAVVYDGADYSRLKIPVANRVKAMFGRSFEF
                     ENETGPGEYYVDSVGVSREHQGKGIGSRLFRFLIEEYVSKKRTALGLLVDKENPTAKK
                     LYLKLGFEKVGDLVLAGKSMEHLQVKHKNIY"
BASE COUNT          808 a          527 c          608 g          794 t
ORIGIN      
        1 accgccttag acccttcatt ggtttgagcg caggtatagg acttccattt agaaaaatgg
       61 tcaatcactc tgtagaaagc gaactgtata ttggaagaca aggtacggag catccgcttt
      121 atctgacgaa tattcaagca gatgcagggc tattgctgga cgacagctgg agtatcaaat
      181 caggtatcga atggcagcag gctgtggaac aattcagcta caaaaaattt gatgctaccc
      241 gcctggaata taaaacagat gatataacgg ggcggataga tgatacttca ctggtaaggg
      301 gcctattgac agaacaattt ggaaataagt acacaatgct caatattcca ttgtacctgg
      361 ggtatgaaaa gcgttttggc aggtgggtgt tcgggattga tggcggtgtc ggttttaata
      421 tacatttcag aacttcggga aaagttcttg ttgatgatca aaaaattcag tcactggaca
      481 acaggcatga tatttatgcc tcatctacag gcatcagctg gagagtaggt gccagctgtc
      541 tgagaagcat tgctcccgga acgatggtgt atgcccgcct gatgtgtatg gcctatcctg
      601 atagttggac cctaagcacc catccaaacg agattcgata tagatttcta accgccaatg
      661 ttggcctgag aaaagttttt tgattagtga aagggctttc ggtaatcaac agcgacctcc
      721 gaccagacat gattggccac tattaaggtt tcgttcttgt cagatataca ttccagaaag
      781 ggatcaatcg taattcctgt ttagttgtga tttcctcatt ggaggatttg actgtccggg
      841 taaatattct tgaaagcttc gggtaaatgt acaggaactg ttgccggaga tattttctgt
      901 tattacctga atggatttgc cggactgaat tcaaattaac tacgttcatc ctttatacat
      961 tacccatctc cataaataaa tctgtcatga cgaaagcttc ctctttgggt tttcagcgca
     1021 ctgcacggcc tttcaacagt agatgtccaa acgataatcg tagccctggc attgtgattg
     1081 aactgtagtt gaaaaacatt tcgaaaggag ggtgccaata tctgaggcca tgtccaactt
     1141 acggatgcaa aacaggggtc aggaaggata agcagaaaat gaccctgaca tttacctgca
     1201 aaaaaatgaa tggttacatc cactcagcat agggatgctc ggccaggaat gcattgtaat
     1261 atctgggatc ttccgtaact tcttcgccca cccaatccgg cttttcgaac aattcatctg
     1321 ctgaattcaa ttctatctct gccacgatga gaccagaatt ttttccttca aagacatcga
     1381 cttcccatgt cttagcccga aatgacataa aatatcgtct ttttgcaacc tcgtatgaac
     1441 aaaataattc cagtagttca ttggcatctt ccctgggaat gggatattca aactcgcttc
     1501 tcactgcacc ctttgactca cccttgatcg taataaagcc actatccgga gtgattctga
     1561 ctcgaacgac cttttgttga tccttgacta tatagccttg tcggaaaggg acaccaacag
     1621 gtctatcgac tttctcccat gcgtcgtgat caacgagaaa tttcctttca atttccaatg
     1681 ccatttttga tatgatgttt gatttcaata aaaagttgta tgacaagata cacatttgtg
     1741 acaaagaaaa gcgataactg atcgaaagag ttatattgat taccttcacc ttcggaacaa
     1801 tcttatttac ctaaaaatgg aaatactgat aagaagggcc tgccctgaag ataacgaaca
     1861 tattgtcgcc catatcatcc tggcgatgga agatatcatc tttcgcttta tcggagaaga
     1921 gtcatcggag aaggcatttg cgtttttaag cagtctggtc aaagagcgag gcaacctgta
     1981 ttcctatgaa aactgctggg ttgctgagca tggaagcgaa gttgtcgcca cggcagttgt
     2041 atatgatggt gctgattata gcaggttgaa aattccggtg gcgaataggg taaaagccat
     2101 gtttggtcgt tcttttgaat ttgagaacga aaccggcccg ggagaatatt acgttgattc
     2161 ggtgggagtc agccgggaac atcagggcaa gggaataggc tcaaggttat tcagattcct
     2221 gatcgaagaa tatgtttcca aaaagagaac tgcactgggt ttattggtag ataaagagaa
     2281 cccaactgca aagaagcttt atctgaaact cggatttgaa aaagttggtg atcttgtgct
     2341 tgccggcaaa tccatggaac atttgcaggt caaacataag aatatttatt aagtgtccgg
     2401 aattagtttg gatccggtgt tttcattgaa aaattagcta aagttggtag tttgctacaa
     2461 tcagacagac atttattcaa aattcgatag aagaatttaa tttactgata ttggatttca
     2521 ttgagcagtc tgcatttgat taaagctaca ccgacaaaag ttcatctttg ctttcactat
     2581 acaatcttgc tcagaaggtc tattgacaga tgaagacaat gtaaggcatt gatctgttga
     2641 ttgatttccc ggttgaaagc gggattgccc gggctttgtt tcttatacga aagttaaaaa
     2701 ataaatagac caaaatttgg tgtattggat tatttgt
//
LOCUS       DAOQMC010000284         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22013_length_2725_cov_3.489139, whole genome shotgun sequence.
ACCESSION   DAOQMC010000284 DAOQMC010000000
VERSION     DAOQMC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22013_length_2725_cov_3.489139"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKV31_09430"
     CDS             <1..591
                     /locus_tag="PKV31_09430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNB93120.1"
                     /translation="LTIEVDMEHAIAAREALNKKGDVKISYNDLVTKATALSLAKHPQ
                     VNSSWMNDFIRENYVVNIGVAVAVPDGLLVPVVRDANFKTLSELNQEIKTLAAKAKEK
                     KLSLEEMQGNTFTISNLGMFDIDNFTAIINPPDACILAVGSIVSKPVVKNGAIVPGNT
                     MKLTLSCDHRVVDGAVGAQFLQTLKSYLEQPLNMLL"
     gene            complement(1061..1543)
                     /locus_tag="PKV31_09435"
     CDS             complement(1061..1543)
                     /locus_tag="PKV31_09435"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNB93121.1"
                     /translation="MEKKIISFEYQIIPDLDGLESDDRALLKRASEALEQAYAPYSEF
                     KVGASLIDSEGNVFIGANMENASYPICICAEGALLTAYNTSGSKAPIRTVAITATSSS
                     HVIDHPIAPCGACRQMLSEMELKQGQAIRLLLQGSEGPVYIIDKISDVLPLGFSGTEL
                     "
     gene            1671..>2725
                     /locus_tag="PKV31_09440"
     CDS             1671..>2725
                     /locus_tag="PKV31_09440"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNB93122.1"
                     /translation="MSDSKSKKTITLFGAGRSATFLIEYLLKHAPSEGWQIILADSNP
                     EVAFSKLGGNPNGSAVSCDINDAVKRRELIAASDFVISMLPAFLHILVAKDCLMYNRD
                     LATASYLSDELKEMEDEIKAKGLVFANELGLDPGLDHMSAMKIFDEIKRKGGQITSFK
                     SFTGGLVAPESDDNPWHYKISWNPRNVVLAGQCTSQYMESGNVHYVPYHRLFRISEDI
                     KVIGHGTYSMYVNRDSIKYIDLYGLEGIQTMIRGTFRGENYCDAWAVLIDLGLTDNST
                     RMSLNKNFTLREYIIGFLSGSGNNYKHRIESTIGRKISDRTFEQIQWLGLFSNDTCPL
                     QHPTPAEILEYIIVNKWV"
BASE COUNT          860 a          573 c          527 g          765 t
ORIGIN      
        1 cttaccatag aagtggatat ggagcatgca attgccgccc gagaagcgtt aaataaaaaa
       61 ggtgatgtaa aaatttcata taatgaccta gtaacaaaag cgactgcgct ctctctggcc
      121 aaacacccgc aagtcaactc aagctggatg aacgacttta tacgtgaaaa ttatgtcgtc
      181 aacatcggtg ttgcagttgc agttccggat ggattgcttg taccggtggt aagggatgct
      241 aattttaaga cattatcaga attaaatcag gaaattaaaa cgttggcagc aaaagccaag
      301 gaaaagaaat tgagcttgga ggagatgcag ggtaatacct tcaccatttc caatctggga
      361 atgtttgata tcgacaactt tacagcaatc atcaatccac ctgacgcatg cattctggct
      421 gtcggaagta tcgtaagcaa acctgtcgtt aaaaatggtg caatagtgcc aggtaacacg
      481 atgaagctta ctctatcctg tgatcacaga gttgtagatg gtgcggttgg tgcccaattc
      541 ttgcaaacgc tgaaatctta tcttgaacag cctttgaaca tgctgcttta agaggaggtt
      601 tctcaggatg tttacattta tttaggcata ggttttaatc caacctcatg attcgaattc
      661 aataaagcca atggattccg gttcttactt ggaggaatta accctaacag ccaccacctt
      721 attgtcttat ccggatacaa agtgctgatt cataagtcaa acacctaagg tattattgaa
      781 atcaagggta gaaattatat accggaagcc atttatacat ttcattatat cgccgtttct
      841 ttctgtaaat taattagtat aaaatccaat aaacacaaag attaaggata taattttcag
      901 ttcaaatcaa cctcaacttt attgataaac tacctataaa atattttctc ttaaaacata
      961 gaattacctt attcgatcgg acaagaatct tctatattat attcaatagc tattccgccc
     1021 ccgaaacttc catttaaaga cggtataatg ttcattttat ctaaagctca gtccccgaaa
     1081 aacctaatgg caatacatcg cttatcttat cgatgatata caccggccct tcactccctt
     1141 gcagtaatag gcggatagct tgaccttgct ttagttccat ttcactcaac atctgacgac
     1201 atgcgccaca tggggcaatc gggtggtcga tgacatggga tgaggaggtg gcagttatgg
     1261 ctactgtacg aataggcgct tttgatccac tggtattata ggcagtgaga agggcgcctt
     1321 ccgcacaaat gcatatcgga taagaagcat tctccatatt ggccccaatg aatacgtttc
     1381 cttccgaatc aatcaaagat gcacctacct taaattcaga ataaggagca taagcttgct
     1441 ccaatgcctc acttgctctt ttcagaagtg ccctgtcatc actttcaagc ccatccagat
     1501 caggtattat ctgatattca aatgatataa tcttcttctc catatagaaa tgttggatta
     1561 aatataataa aatagttgat gatgttaata taaaaatacc agcaaattca atcaactaag
     1621 gttaaattaa taaattcgca ccttcaaaac cagattttca accggctctc atgtcagatt
     1681 ctaagtcaaa aaaaacaatc actctctttg gtgcagggcg atctgctaca tttctaatcg
     1741 aatacctact caaacatgcg ccatctgaag gttggcaaat tatccttgca gacagcaacc
     1801 ctgaagtagc tttttccaag ctcggcggaa atccaaacgg ctcagctgtc tcctgtgata
     1861 tcaatgatgc cgttaaaagg agagaattga tagccgcatc ggattttgtt atctccatgt
     1921 tgcctgcctt cttacatatc ttagtagcta aggactgttt gatgtacaac agggatttgg
     1981 caacggcatc ctatctgagc gatgaactaa aagaaatgga ggatgaaatc aaagccaagg
     2041 gccttgtttt cgccaacgaa ttgggtttgg atcccggtct ggaccatatg tcagccatga
     2101 aaatctttga cgaaatcaaa agaaaaggtg gtcaaatcac ttcattcaaa tcctttaccg
     2161 gaggccttgt tgcacctgaa agcgatgaca acccatggca ttataagatc agctggaatc
     2221 ccaggaatgt cgtcctcgca ggtcaatgca catctcaata catggagagt ggcaatgtac
     2281 actatgtccc ttaccacaga cttttcagga tatcagaaga tataaaggtt ataggccacg
     2341 gcacctattc tatgtatgtc aaccgagatt ccatcaaata tatcgacctt tatggactgg
     2401 agggcattca aaccatgatc agaggaacat tcagggggga aaattattgt gacgcctggg
     2461 ctgttcttat cgacctcgga ttgactgaca attcgacaag aatgagttta aataaaaatt
     2521 tcacgctgag agaatatatt ataggcttcc tgtcaggatc cggaaataac tataagcacc
     2581 gaatcgagtc tacaataggc agaaaaatta gcgaccgcac atttgaacaa atccaatggc
     2641 tgggcctctt cagtaacgat acttgtcctc tccagcatcc tactcctgct gaaatccttg
     2701 aatatatcat tgtaaataaa tgggt
//
LOCUS       DAOQMC010000285         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22040_length_2722_cov_6.416948, whole genome shotgun sequence.
ACCESSION   DAOQMC010000285 DAOQMC010000000
VERSION     DAOQMC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22040_length_2722_cov_6.416948"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PKV31_09445"
     CDS             <1..1071
                     /locus_tag="PKV31_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNB93123.1"
                     /translation="QILNTFCFYGADPRILIDANPHIGTNKLPKIITAMREAILKAGG
                     RILFEHRMVDFDIEDGRLAKVHFRHQPSIECKAMILATGHSARDIYRLLANRGLKIEA
                     KPFAMGVRVEHPQSLIDSIQYSCPTRPEGLPAAPYSVVSQEGGRGVFSFCMCPGGMIV
                     PAATSPGELVVNGMSPSRRDSPYANSGFVTSVELEDIPAEYGEDAFVAMNFQSSVEQR
                     LFNYGNGTQSSPAQRLVDFTRKKESTDLPNCSYVPGIYPAPMHRILPEFIVDRLISGF
                     LSVGKKLHGYLTNEAVVVGVESRTSSPIRIPRDKESLRHPDLVNLFPCGEGAGYAGGI
                     VSAGIDGVNVAVAVSKYLTMEG"
     gene            complement(1142..2149)
                     /locus_tag="PKV31_09450"
     CDS             complement(1142..2149)
                     /locus_tag="PKV31_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNB93124.1"
                     /translation="MELGVGMFGDIPFDESGNPGKADQKLREIISAIQFADELGLDVF
                     AIGEHHRPDYAVASPEIVLAAAAPVTKQIKLMSAVTVLSSSEPVKVYQDFSTIHLLSA
                     GRAEIGADRGSFIESFPLFGYDLKDYDELFEEKLNLLLKIRNETNVTWTGNLRPPMIN
                     QTVFPRSENLPIWIAAGGTPSSVLRAARLGLPLIVAIIGGNPAQFSPLLEYYREEYIS
                     YGHDPAKMQLAVHAHTFVSENQKDIDNYFHYYSRQMNRIGQSRGWPTFTHGQYEAGRS
                     KHGAYLFGSSQEVSEKILMMKEVFGLTRFIGHIDVGAPPHDMLMNTIELMGTKVVDKV
                     K"
BASE COUNT          778 a          573 c          608 g          763 t
ORIGIN      
        1 cagattctga ataccttttg tttttatgga gctgacccaa gaatactaat cgatgcgaac
       61 ccccatatcg gcactaacaa attgcccaag attattaccg ctatgcggga agctatattg
      121 aaagcaggtg gaaggattct gttcgaacac agaatggttg attttgatat tgaagacgga
      181 agattagcca aagtacactt tcgacatcaa ccttccatcg aatgcaaggc catgatactc
      241 gccacagggc atagtgctcg tgatatttac agattgcttg ccaatagagg attgaagatc
      301 gaagcaaaac cgtttgcgat gggagtacgt gtggagcatc cccaatcatt gattgacagt
      361 atccagtata gttgcccgac cagacctgaa ggtctgcctg ccgctccata tagtgtggtg
      421 agccaggaag gtgggcgggg cgttttttca ttctgtatgt gtccgggtgg gatgattgtc
      481 ccagcagcaa ccagtccggg agagctagtc gtaaatggca tgtctccatc ccgaagggac
      541 agtccctacg ccaatagtgg cttcgttacg tctgtcgagt tggaagatat ccctgcagaa
      601 tatggagaag atgccttcgt agccatgaat ttccaatcat ccgtagaaca gcggctattc
      661 aattacggca atggtacaca gtcaagcccg gcacaaagac tggtcgactt cacacgtaag
      721 aaggaaagca cggacctgcc caattgcagt tatgtgcccg gaatctatcc ggcaccgatg
      781 cacagaatat taccggaatt tattgttgac aggctgattt caggattttt atcagttggc
      841 aaaaaactac atggttactt gaccaatgaa gcagttgtcg taggtgttga aagcagaacc
      901 agttctccaa tccgcatacc aagggataag gaaagtctcc gacatcctga cctggtcaac
      961 ttatttccct gtggagaagg ggcggggtat gcgggaggca tcgtatcggc aggaattgac
     1021 ggtgtaaatg tggccgtagc agtaagtaaa tatcttacta tggagggatg aaatccggaa
     1081 ttgttgggtt ggaagtttgg tttgccattc aggccgtttg ttttagaaat ttggtaaggc
     1141 attattttac tttgtctaca acttttgtac ccatcaattc aattgtattc atcaacatat
     1201 catggggagg agctccaaca tcgatgtgac ctatgaatcg ggtcaagccg aaaacctcct
     1261 tcatcatcag gattttttca gaaacttcct gtgagctacc aaataaataa gctccatgtt
     1321 tggacctgcc ggcttcgtac tgaccatggg tgaaggttgg ccagccacga ctttgaccga
     1381 tacgattcat ttggcgacta taataatgaa agtaattatc gatgtccttt tggttctcac
     1441 ttacaaatgt atgggcatgt acagccagtt gcatctttgc cggatcatga ccataggaga
     1501 tatattcctc cctgtaatat tccaaaagag gagagaattg ggccggattg ccgccaatta
     1561 ttgcaacaat caaaggtaag cccaatcggg ctgcacgaag aacagaagaa ggcgtgcctc
     1621 ctgctgcaat ccatatgggc aggttttcag agcgaggaaa aacagtttgg ttgatcatag
     1681 ggggtcgaag attacctgtc caagtgacat ttgtttcatt tctaattttt aacaaaagat
     1741 tcaacttctc ttcgaacaat tcatcatagt ctttcaagtc ataaccaaat agaggaaagg
     1801 actcgatgaa gctaccacgg tctgccccaa tttctgctcg accagcagaa agcaaatgaa
     1861 tggtggagaa atcctgatat acctttacag gttcagaact gctcaacacc gtaaccgcag
     1921 acattagttt gatttgtttt gtgaccggag cagcggctgc caatacaatt tctgggctag
     1981 caacagcata atccggtctg tgatgttcac cgattgcaaa tacatccagt cctaattcat
     2041 ctgcaaattg aatggctgaa atgatttccc ggagcttctg atctgctttt ccgggattgc
     2101 cggattcatc aaatggtata tcaccaaaca ttcctactcc caattccata atcaataact
     2161 ttattttgtt cttagaataa tataaggggg attatcattc ttagaataat gtaatggtaa
     2221 atcaaatgtg tttgttttac taaaatagtt attgcctgtt ttatttcgtc tatcaaggct
     2281 tgtaaacaaa tattttcaga aacatttcaa agaatgcgtc attgtataaa cctcgattat
     2341 atagaagtat aacaaaaaga aaataatact gaatgcacgt tcgtaacaga ccaatttcat
     2401 tggtctataa cgaatgtaac ctcggcatcc cggaatcccg gattgtttca ctcccgggac
     2461 agagcagagc tctaatcgcc tgactctcgg gacaagtata ccaaaaatat ttggattatt
     2521 ttataattga tcttggtata aaatataggc caaggcactt atcctgtttt taacatctta
     2581 ccggtcatcg gcctatgcct gaccattctg gagatgatgt catccaggat ttcacctttc
     2641 atgcggtgcc tgttgtagcg gtagcaatat tcattgagat agtcctgcag ataggtcaca
     2701 tggccgtgtc tggcatatag cc
//
LOCUS       DAOQMC010000286         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22094_length_2718_cov_4.874578, whole genome shotgun sequence.
ACCESSION   DAOQMC010000286 DAOQMC010000000
VERSION     DAOQMC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22094_length_2718_cov_4.874578"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2718
                     /locus_tag="PKV31_09455"
     CDS             <1..>2718
                     /locus_tag="PKV31_09455"
                     /inference="COORDINATES: protein motif:HMM:NF037268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HNB93125.1"
                     /translation="NFQSAQNFIRISATWVKIWQNLSNVIIRKKGSPALKKKKPSSGD
                     SSLHEPEEYIFDLDARVSLFEELNIKGNLTAKKALELLGHDANQWEVNFVKLEGNHTN
                     QLLYKAYLDILEYEGYDIKELLHVKSNKDEVDLDDLNVPALQIKNMIKDVFGALKIDT
                     SILDFNAELDGVDFEQQPAYQLWHLLYSYEGDDSNSGNERLYRLLNKKFGFTTEHAKI
                     LAGISFQNDYGSLSTKAIRRIYPYLKENKYSDACVLAEYRHSKNSLTKEELESRELKD
                     RLEPIKKNSLRQPVVEKILNQMVNVVNALIDKENDQLEASGHQRNFHFDEIRIELARE
                     LKKNAAERAEMTSNINAAKLNHDKIVKILQKEFGILNPSRNDIIRYKLYEELKNNSYK
                     DLYSNRYIAKEDLFTNKIDIEHIIPKSRMFDDSFSNKTLAFRDVNLRKGEKTAFDFMQ
                     IDTDDEGFELYLSRVENLYKLGMRSPEEGISKAKYKKLLKKESELDDGFIERDLRETQ
                     YIAKKARQMLFEVTRSVISTTGSITDRLREDWGLINVMKELNFQKYKALGLTETLERR
                     SGQKIEVITDWTKRNDHRHHAMDALTIAFTRHSHIQYLNYLNARKDEHHKLHSVILAI
                     ENKETLSRPDADGSKKRVFKEPITNFRKIAKEHLEDIIISHKAKNKVVTKNKNKIAKN
                     NHVQTTLTPRGQLHKETVYGQYRYSVKKEESVGAKMTYDTIMKVTKPAYRNALLERLK
                     ESGGDPKRAFTGKNAISRNPIFLNEAKTECLPEKVQLVWLEKDYSIRKDVNPDNFKDE
                     KLIDKILDEGIKRIMKERLRQYGGDPKKAFTDLANNPIWLNKEKGISIKRVTISGVSN
                     AEPLHYKKDHHGQFLVDENGNNIPADYVSTGNNHHVAVYRDENGNIQEN"
BASE COUNT          993 a          456 c          592 g          677 t
ORIGIN      
        1 gaaacttcca atctgcgcag aatttcatcc gcataagcgc aacttgggtt aaaatctggc
       61 aaaatttatc aaatgtaatt attcggaaaa aaggaagtcc tgcattaaaa aagaaaaagc
      121 catcttccgg tgattcttca ttgcatgaac cggaggagta tatatttgac ctcgatgcca
      181 gggtcagctt atttgaagaa ttgaacataa aaggcaacct gactgcaaag aaagcccttg
      241 aattgcttgg acatgatgcg aatcaatggg aagtaaactt tgtcaaactg gaaggaaatc
      301 ataccaatca attactctat aaggcgtatt tagacattct tgaatatgaa gggtatgata
      361 ttaaggagtt gctgcatgtc aagtcaaata aggatgaggt tgacctggat gacttaaatg
      421 tgccggccct tcagattaaa aatatgatca aagacgtttt tggtgcctta aaaattgata
      481 cctcgattct tgattttaat gcggaattgg atggtgtcga ttttgaacag caaccggcat
      541 accagttgtg gcatcttttg tattcatacg aaggcgacga ttctaatagt ggcaatgaaa
      601 gattgtaccg gttgctgaat aaaaagtttg gatttacaac agagcatgct aagattctgg
      661 ccggaatatc ttttcaaaat gattatggta gtctgtccac aaaggctatc agaaggattt
      721 atccctatct gaaagagaat aaatatagcg acgcttgtgt tttagccgaa tacagacatt
      781 ctaaaaattc gctgacaaag gaggaacttg aatccagaga gcttaaagac aggctggaac
      841 cgataaaaaa gaatagcctg agacaacctg ttgttgagaa aattctcaat cagatggtca
      901 atgttgtcaa tgccttgata gataaggaaa atgatcaatt agaggcatca gggcatcaga
      961 gaaactttca ttttgacgaa ataaggattg aattagccag agaattaaag aaaaatgctg
     1021 ctgaaagagc agaaatgacg tcaaatatca atgctgcaaa gctcaatcac gacaaaatcg
     1081 tcaaaatact gcaaaaagaa tttggcattc tgaatccttc acgaaatgac atcatccggt
     1141 ataagctgta tgaggaattg aagaataata gttataagga cctttattcc aacaggtata
     1201 ttgccaaaga agaccttttt acaaataaaa tagatattga acacatcatt ccaaagtccc
     1261 ggatgtttga tgacagcttc tccaataaaa cactggcttt cagagatgtt aatttgagga
     1321 agggggagaa gacagctttt gatttcatgc agatcgatac tgatgatgaa ggatttgagc
     1381 tttatttaag ccgggtcgaa aacctatata agctggggat gaggagtcct gaagaaggca
     1441 tttccaaagc aaagtataaa aaactgttga aaaaagaaag tgaactggat gatggcttca
     1501 tagaaagaga tcttagagaa acccaataca tagctaagaa agccagacag atgctttttg
     1561 aagtaactcg gagtgttatc tcgacaacgg gaagtattac tgaccggtta agggaagact
     1621 ggggactgat caatgtaatg aaggaactca attttcaaaa gtataaggca cttggcttaa
     1681 ctgagacact ggagaggagg tcggggcaaa aaatcgaagt tattacggac tggacgaagc
     1741 ggaatgatca tcggcatcat gccatggatg cactcaccat agcatttaca aggcacagcc
     1801 atatccagta tctcaattat ctgaatgcaa ggaaagacga gcatcataaa ctgcacagcg
     1861 taatcctggc tattgaaaat aaagagacct tatcaagacc ggatgcggat ggaagtaaga
     1921 aaagagtctt caaagagcca attacgaact ttcggaagat agccaaagag catctggagg
     1981 atattattat ttcccataaa gcaaaaaata aggtagttac caaaaataag aataaaatcg
     2041 ccaaaaataa tcatgtacaa acaacgctca cacctcgtgg ccagcttcat aaagaaactg
     2101 tttacggaca atacagatat tccgttaaaa aagaagaatc tgtgggagct aaaatgactt
     2161 atgataccat catgaaggtt accaaaccgg cttatcgaaa tgcccttttg gaaaggctca
     2221 aagaatctgg tggtgatccc aaaagagcat ttaccgggaa aaatgcaata tccagaaatc
     2281 cgatatttct caatgaggcc aaaacagaat gcctccccga aaaggtgcag ttagtttggc
     2341 tcgagaaaga ttattctatt cggaaagatg tcaatccgga taattttaaa gatgaaaaac
     2401 tgatagacaa aatcctggat gaaggtatca aaagaataat gaaagaaagg ctgagacagt
     2461 acggtgggga tcccaaaaaa gcatttacag acctggcaaa caatccgatt tggctgaata
     2521 aggaaaaagg tatttcaata aagagggtta caattagcgg agtgagtaac gccgagcctc
     2581 ttcactataa aaaagatcat catggtcagt ttttagtcga tgaaaatggc aataatatcc
     2641 ctgcagacta tgtcagtacc ggaaataatc atcatgtggc agtctatcgg gatgaaaatg
     2701 gaaatattca ggagaatg
//
LOCUS       DAOQMC010000287         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22190_length_2711_cov_4.606928, whole genome shotgun sequence.
ACCESSION   DAOQMC010000287 DAOQMC010000000
VERSION     DAOQMC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22190_length_2711_cov_4.606928"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1329)
                     /locus_tag="PKV31_09460"
     CDS             complement(13..1329)
                     /locus_tag="PKV31_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93126.1"
                     /translation="MIKKILGLDLGTTSIGWAYVLEDEDYTNSEIKQIGVRVNPLTVD
                     EQTNFEKGKPFSVNAGRTLKRGMRRNLDRYQDRRKRLKQLLLQGGIIQGDSILSENGK
                     NSTHSTWELRAKAVEEKVDLVDLAKILMAINKKRGYKSSRKANKEDEGTAVDGMAIAI
                     KLYEEKLTPGQLVFDQLIRGKKNIPDFYRSDLQSEFDLIWKCQKQFYPDIFTDAFYES
                     ISGKGMRATSAAFWNTYKFNTADIKDIDDNLKNQFTYQYNLRDKKKLQAYKWRSDAVS
                     KQLSKEQAAYVITEINSNINNSSGYLGDISDRSKELYFNKETIGQYLYRQLKKDRHAR
                     LKSQVFYRQDYLDEFEKIWETQAKFYSQLTPQLKEEIRDTVIFYQRKLKAQKGLISFC
                     EFESKEIEINKDGKIIKKTIGQRVIPRSSPLFQVFNPSCALFNKLC"
     gene            complement(1467..2372)
                     /locus_tag="PKV31_09465"
     CDS             complement(1467..2372)
                     /locus_tag="PKV31_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNB93127.1"
                     /translation="MDDTRPVRTLRLLMMLSGSRRYTMAELMDKFAMSERNLRRDLLL
                     LENAGFVLDRKNGYRIVLEGNRSGVLKKILHFSEEEIALIYGALTTLDAASSIKARLL
                     KKMNAFYDLKVIEKLKEKDDLQKVSLLRRSMEQGKQVILSNYRSSNSQTIEDRQVEAF
                     DFGPDYETVWCYDLRDHECKQFKVTRMSGVCLTANDWKYKGSHRRPFTDIFRVSAPEA
                     IDRVKLRLSLRAYNLAVEEYPLANDYIRQAESSYLLEAPIAEYSGVGRFVMGFLDEVQ
                     VLESEGLKEYINRLIRSYDVGNVWK"
BASE COUNT          721 a          627 c          486 g          877 t
ORIGIN      
        1 tttgaccgtt ttttaacata atttgttgaa taatgcgcaa cttgggttaa atacctgaaa
       61 taagggtgaa gacctgggta tgactctttg cccgatcgtc ttttttatta tctttccgtc
      121 cttgttgatt tctatctctt tgctttcaaa ttcacagaaa cttatcaggc ctttttgagc
      181 cttcagttta cgctgataga agattacggt atctctgatt tcttccttaa gctgaggagt
      241 caactgagaa taaaactttg cctgtgtctc ccatattttt tcaaactcat ccaaataatc
      301 ctgtctgtaa aacacctgac ttttgagcct tgcatgcctg tcttttttca gttgcctgta
      361 tagatactga cctatagtct ctttattaaa atacaattcc ttgcttctgt cagaaatatc
      421 cccgaggtag ccgcttgaat tattgatatt actattaatt tcagttatta cataggcagc
      481 ctgctctttt gaaagttgtt tactcacggc atcacttctc catttgtagg cctgtaattt
      541 tttcttgtcg cgaaggttat attgataggt aaattgattt tttaagttgt catcgatatc
      601 tttaatgtca gctgtattga atttataggt attccaaaat gcagcagaag tagctcgcat
      661 tcctttacca ctaatactct cataaaaggc atcggtaaaa atatcaggat aaaattgctt
      721 ttgacatttc cagattaagt caaactcact ttgcaaatct gatcggtaga aatctggaat
      781 attctttttt ccacggatta attgatcaaa aacaagttgc cccggagtca gcttttcctc
      841 ataaagcttt atagctatcg ccatcccgtc cacagctgta ccctcgtctt ccttattagc
      901 tttccggctg cttttatatc cgcgtttctt gttaattgcc attaaaattt tagccagatc
      961 cacgaggtca accttctctt caacggcttt ggcacggagt tcccaggtag aatgtgtgga
     1021 attttttccg ttttcagaca gtatagaatc tccttgaatt attccgccct ggagtaaaag
     1081 ttgttttagt cttttccggc gatcctggta cctatccagg tttctgcgca ttcctctctt
     1141 taatgtcctg ccggcattga cagaaaatgg tttccctttc tcaaagttgg tttgttcatc
     1201 gactgtaaga ggattgaccc tcactcctat ctgttttatt tcagaattcg tataatcttc
     1261 atcttcgaga acataggccc agccaatgga agtagtcccc aagtcaagtc ctaaaatttt
     1321 ctttatcatt ttatctggtc tttaatcaac taatttatat atttgcctta caaaagcaat
     1381 tcacaataag gatttttccg ttgtgaagac atctagcgcc tcgcctatct gcggggcttt
     1441 tttatgcctg ttgcacaaat ctaattttat ttccacacat tcccaacatc ataggatcgt
     1501 atcaaccggt tgatgtattc cttcagccct tcactctcca gtacctgcac ctcatccaga
     1561 aaacccataa caaaccggcc tacaccacta tattcagcga tgggcgcctc aagcagatag
     1621 gatgattcgg cctgcctgat gtaatcgttg gcaagcggat attcttctac cgcaaggttg
     1681 taagcacgca ggctgagccg gagctttacc cggtctatgg cttccggagc agatacccgg
     1741 aagatatcag taaacggacg ccggtgactc cctttatact tccaatcatt agccgtaaga
     1801 cagacacccg acattcttgt tactttaaat tgtttgcact catggtcacg gagatcataa
     1861 caccagacgg tttcataatc cggaccaaaa tcaaacgcct ctacctgcct gtcttcgatc
     1921 gtctgactat tggatgatcg gtaattgctc agaattacct gcttgccttg ctccatcgat
     1981 cttcgcaaca aactgacctt ttgaaggtcg tctttctcct tcagcttttc gattaccttg
     2041 agatcataaa aggcattcat tttcttcaaa agtcgtgcct tgatagaaga agccgcatcg
     2101 agagttgtaa gcgccccata aatgagggcg atctcttctt ctgaaaaatg aagaatcttc
     2161 tttagcaccc cggaacgatt tccttcaagc actatgcgat atccattctt ccggtcaagg
     2221 acaaagccgg cattctcgag caataacaag tctcgccgca gattgcgctc cgacatggca
     2281 aacttatcca tcagctcggc catcgtatat cgacgcgaac cggagagcat catcaataat
     2341 ctgagtgtcc ggacaggtct ggtatcatcc attttgttca atttagtcgt gattgcctca
     2401 tatcgacaaa ataaggttag tacggataaa cttaaggtat ttattggatt ttttcttgaa
     2461 ggggccggag attttctact aaagaagcaa atccccggca aatttatatt aagggtaaca
     2521 cctgatgaaa tcaactctcc ccgatgctgg cgcaaagcta aatcctacac aggacaaaac
     2581 ctgtccgtgt aagtcattcc tctcacaaaa tctgaagttg agatattata acagaatcac
     2641 aaggaataac agggtcaata atctcggtca atgattttcc aatccaatat ttccatggac
     2701 gaatattttg t
//
LOCUS       DAOQMC010000288         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22235_length_2708_cov_4.718809, whole genome shotgun sequence.
ACCESSION   DAOQMC010000288 DAOQMC010000000
VERSION     DAOQMC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22235_length_2708_cov_4.718809"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PKV31_09470"
     CDS             complement(<1..1149)
                     /locus_tag="PKV31_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB93128.1"
                     /translation="MRLFFISLVIVLAGTLYSQELWNPVEFNTREETRIKVSKFDFYK
                     LDLTLLKESLTKASNEFQRDNQGVIIEIPMAGKSPQRFRVKQTNTIHESLRSKYPGID
                     NFVIQSVDNPRIHGRIDYTYQGFHAIIKDGSSTTFIDPAFEHNGDYYMVYTRREAINT
                     KSFECYFSKEDDFKPYERMGVSFSDNMLRTYRLALSCTGEYAQFHGGTTPLVLSAMNT
                     TINRVNFVYELEYSVRFEIIPNTDVLIYLNPITDPYDNDDIGKQLDQNQIECDTKIGA
                     ANYDIGHIFSTGGGGLAGYAVVCKNNKAWGGTGSSKPINDPFDIDYVAHEIGHQLSGS
                     HTQNNSCNRDNSACYEPGSASTIMGYAGICSPNVQNNSDAYFHGYSIQE"
     gene            complement(1217..1903)
                     /locus_tag="PKV31_09475"
     CDS             complement(1217..1903)
                     /locus_tag="PKV31_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNB93129.1"
                     /translation="MHLTESEEDHIRIIFNLYERTGKPVSTNAIAAGMKTTAASVTDM
                     LKRLQVKQLIDYQKYYGVTLNTRGNALAIHLIRRNRLWNVMLRDKLHVKWDQIESITD
                     QLEHINSDMLIEELDRYLGYPQNDVFGDPIPDSNGKFVLRARYPLNEVSFGQQLTVVG
                     ILNHSAEFLKLIDVANIQIGSEIIILEKFDFDQSYLILHKGIEKQLSISIARNLLVRY
                     NNRINHPGKK"
     gene            2018..>2708
                     /locus_tag="PKV31_09480"
     CDS             2018..>2708
                     /locus_tag="PKV31_09480"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNB93130.1"
                     /translation="MKTKEEIINNWLPRYTGRSLEEFGKFILLVNFKDYVVRFANTYG
                     VPLYGEDRAMQSASFDDVTIINFGMGSPNAATIMDLLEAITPKAVLFLGKCGGLKKVN
                     KVGDLILPIAAIRGDGTSDDYFPPEVPALPAFALQKAISTAIRENERDYWTGVVYTTN
                     RRVWEWDDNFKDYLFSIRAMGIDMETATIFSVGFHNRIPTGALLLVSDVPMFPEGVKT
                     DKSDMHVNANFV"
BASE COUNT          785 a          555 c          520 g          848 t
ORIGIN      
        1 ctcctggatg ctgtatccat ggaaataagc atcgctgttg ttctgaacat ttggtgagca
       61 gatcccggca taacccatga ttgtagatgc gcttcccggc tcataacagg cagaattatc
      121 cctgttgcaa ctattgttct gggtatgtga cccacttagt tgatgaccaa tttcgtgcgc
      181 aacatagtca atatcgaatg gatcattaat gggtttggaa gaacctgtgc ctccccaggc
      241 cttattattc ttgcatacta ccgcataacc tgccaatcca cctccaccag tgctgaaaat
      301 atgaccaatg tcatagttgg cggcccctat cttcgtatca cattctattt gattctgatc
      361 caattgcttc ccgatatcat cattatcata tgggtcagta ataggattta aatatataag
      421 cacatcggta ttaggaataa tctcaaacct tacgctatat tccagttcat atacaaaatt
      481 aactctgttg atcgtggtat tcattgcaga taataccaat ggcgtagtac ctccatgaaa
      541 ctgcgcatat tcaccggtac aggaaagagc caaccgatag gttctaagca tattgtcaga
      601 gaagctgaca cccatccttt catatggttt aaaatcatct tctttggaaa aataacattc
      661 gaaagacttt gtattgatgg cctcccgtct agtatatacc atatagtaat caccattatg
      721 ttcgaatgca ggatcaatga aggtggtact acttccatcc tttatgatgg catggaatcc
      781 ctggtaagta taatcaatcc tcccatgaat ccggggattg tctaccgact gaataacaaa
      841 attatcaata cccggatatt tactccgcag actttcatga attgtatttg tctgcttgac
      901 tctgaacctt tgtggactct tccctgccat aggtatttca ataatgaccc cctgattgtc
      961 ccgttgaaat tcgttggagg ctttcgtcaa actttccttc aacaacgtga ggtcaagctt
     1021 gtaaaaatca aatttactga ctttgattct tgtctcttct cgcgtattaa attcgactgg
     1081 attccacaat tcctgactgt aaagggttcc ggcaagtaca attaccaaag aaatgaaaaa
     1141 taaacgcatt cttgttgtat tataggtttc tgttaattaa aggtaaatat atgaagaatt
     1201 tatgctcatg aacattttat ttcttcccgg gatgatttat tctgttattg tatctgacta
     1261 aaagattgcg ggcaattgaa atcgacaatt gtttttcgat tcctttatga agaatgagat
     1321 aagactggtc aaagtcaaat ttttccaaaa ttattatttc actgccgatt tgaatatttg
     1381 caacatcgat caatttcagg aattcagcac tatgatttaa aatacctact acggtgagtt
     1441 gttgtccaaa actaacttca ttcaatggat atcttgcccg caagacaaac ttgccgttag
     1501 aatccggaat tggatcgcca aatacatcat tttgaggata ccccaaatac ctgtccagtt
     1561 cttcgattaa catgtcactg ttgatgtgct ctaattgatc ggttatagat tcaatttggt
     1621 cccatttgac atgaagttta tccctgagca taacattcca caatcggttt ctgcgtataa
     1681 gatgtattgc caaagcattc cccctggtat tgagagtgac tccataatat ttttgataat
     1741 caatcaattg tttaacctgc aatcgtttga gcatatcagt cacagatgct gctgttgtct
     1801 tcatacctgc agcaattgca ttggtcgaca cgggctttcc ggtcctttca taaagattga
     1861 aaattatcct gatatgatct tcttctgact ccgtcaaatg catactgaca ctgaatttgg
     1921 aaggttgcaa gttagaaaaa aaagagccaa cattccaggt ttatttccaa gtctttgaat
     1981 atttttataa tttgccactt ttaaaaagca aatactgatg aagacaaaag aggaaattat
     2041 caataactgg ttacccagat acaccggacg atctttagaa gagtttggta aatttatttt
     2101 attggtcaat ttcaaggatt acgttgtacg atttgccaat acctatgggg tacctttgta
     2161 tggtgaagac cgtgctatgc aaagtgcaag ttttgatgat gtaaccatca tcaatttcgg
     2221 catgggaagt ccaaatgcag caacgatcat ggacctgctg gaggccatca ccccaaaggc
     2281 tgttttgttt ttgggtaaat gtggcggcct gaagaaggtg aataaagttg gcgaccttat
     2341 ccttcctata gctgccatca gaggtgacgg tacttcagat gattactttc cacctgaagt
     2401 gcctgctctt ccggcattcg cattgcaaaa agcgatttcc acagctattc gtgaaaatga
     2461 acgtgattat tggacaggtg tcgtatatac aaccaacaga cgtgtttggg aatgggatga
     2521 caacttcaag gattatctct tttcaatcag agcgatggga atcgacatgg agaccgcgac
     2581 tattttttcc gtaggatttc acaacagaat accgaccggg gcactgctac tggtgagtga
     2641 tgtccccatg ttccctgaag gcgtaaagac ggacaaaagt gatatgcacg tcaatgctaa
     2701 ttttgtag
//
LOCUS       DAOQMC010000289         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22384_length_2699_cov_4.474281, whole genome shotgun sequence.
ACCESSION   DAOQMC010000289 DAOQMC010000000
VERSION     DAOQMC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22384_length_2699_cov_4.474281"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PKV31_09485"
     CDS             complement(<1..1208)
                     /locus_tag="PKV31_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB93131.1"
                     /translation="MKKSTIWILVTLMSIALFGLTGIQYYWISSAVKTAEEKFQTDVF
                     DVLNKVADQLRNDERYRMYQETFGSRSGSRSMATEFEYESLRRMFNETSLGERIKVKQ
                     LNYYIKTFLADKNIREEYDYGVYSQTAKSFVILNGYYAVTNNNKNVYHDNMDQGLFNT
                     PYKVSIFNDSGSLEHGELMIYFPHRSSFRNLWGIVLSSALFTAIILFTFIFTIYEIVT
                     QKKLSIMKNDFLNNMTHEFKTPIATISLATDSISNDNIINSPDKVRRFLDIIKQENNR
                     MLSQVEKVLQMALIDRKKMKLNLSEIHLHDVIKSAVTHINLQVEQKGGYIETFDEADP
                     DIIQGDITHVSNMVHNLLDNANKYSPEYPEITVRTRNVADGIQVIVTDKGIGMNKEAR
                     KHIFDKFYRAT"
     gene            1371..>2699
                     /locus_tag="PKV31_09490"
     CDS             1371..>2699
                     /locus_tag="PKV31_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNB93132.1"
                     /translation="MNYLTLEHIKKSYGDKVLFDGLSMSINEGHKIALVARNGTGKTT
                     LLQVIAGLEGSEGENAKILFKKGLRIEYLTQDPSFNPEHTVIDAVFDSDNQTLIAVKE
                     YERALLTPGNEARLQAAVNDMEDKKAWDVEARVKEILTKLKIGNFDQKVNQLSGGQKK
                     RLALAKVLISEPELLILDEPTNHLDMEMIEWLEEYLQHPGLTLLLVTHDRYFLENVCI
                     QILELENGNLYKYSGNYSDYLEKKHAKAENEAIVLDKNKKLFLKELEWVRRMPQARTT
                     KAKARIDKFDELKNELSQQREAAILQINIKSNRLGSKIIEAHNVGKSYNDRHLFSHFN
                     YKFNRFDRVGIIGPNGSGKSTFLNILTGNLKPDEGKIVIGDTVVFGYYTQSGINLKED
                     KRVIDVITDVAEYIPTEKGHNITAASLLERFMFPREQQQVYVSQLSGGEKR"
BASE COUNT          788 a          608 c          586 g          717 t
ORIGIN      
        1 gtagcccggt aaaacttgtc aaaaatatgc ttgcgggcct ccttgttcat tccgatgccc
       61 ttatcggtga cgataacctg tataccatcg gcaacattgc gtgtccttac cgtaatttcc
      121 ggatactccg gtgagtattt gttggcattg tccaatagat tgtgaaccat gttggagaca
      181 tgggtgatat ctccctgaat gatatcgggg tcggcttcgt cgaaggtttc gatgtaacca
      241 cctttttgtt ccacctggag attgatgtgt gtcacggcag acttaatcac atcgtgcaaa
      301 tgaatttccg acaggtttag cttcattttc ttccggtcga tgagtgccat ctgcaatacc
      361 ttctctacct ggctcagcat ccggttgttt tcctgtttga tgatgtcaag gaagcgtcgc
      421 accttgtcag ggctattgat gatattgtcg ttggaaatac tatctgtggc aaggctgatc
      481 gtggcgatgg gcgtcttaaa ctcatgcgtc atattgttga ggaagtcgtt tttcatgatc
      541 gacaacttct tttgagtcac aatctcataa atggtaaata taaaagtaaa caatataatt
      601 gccgtaaaca atgcggaact tagtacgatg ccccacaggt ttcggaaaga tgatctgtgt
      661 ggaaaatata tcatcagttc tccatgctca agactgccgc tgtcattgaa aatcgaaact
      721 ttatagggtg tattgaacag accttgatcc atgttgtcgt gatagacatt cttgttattg
      781 ttggtgacgg cataatatcc gttgaggatg acgaaactct tcgctgtctg gctgtacaca
      841 ccataatcat attcttcccg gatattttta tcggccagaa aagtcttgat ataataattg
      901 agctgcttga ctttgattcg ttcacccaaa ctggtctcat tgaacatccg acgcaggctt
      961 tcgtactcga attcagtcgc catagaacga ctcccagaac gactgccgaa agtttcctga
     1021 tacatgcggt atctttcatc attccgtaac tggtctgcca ccttgttgag cacatcaaaa
     1081 acatccgtct ggaacttttc ttcagcagtt ttcacggcac tggaaatcca gtagtattgt
     1141 atccctgtca gcccaaacaa ggctattgac atcaaagtca ccagtatcca gatcgtgctt
     1201 ttcttcataa attatgatgg tatattggac ctgatctttc aggatattgc tctatctcca
     1261 ctatataacg gagtcaaaga taggaatatg tcaaacttac acatatttcc ggaaatatac
     1321 agccgaaaaa tccacgattc ttttgctaat aaatacctta ctttgcgcct atgaattatc
     1381 tgacactcga gcatatcaag aaatcctacg gtgacaaagt cctgtttgac ggtctgtcta
     1441 tgtcgatcaa tgaagggcac aaaatcgccc ttgttgcccg aaacgggaca ggcaaaacga
     1501 ccctgctgca agtaatagcc ggtttggaag gcagtgaagg cgaaaatgca aagattctct
     1561 tcaagaaagg cctccgtatc gagtatctga cacaggatcc atcatttaat cctgaacata
     1621 ccgtgatcga tgcggtattc gacagtgaca atcagacact cattgcagtg aaggaatatg
     1681 aaagggccct gctcactccc ggcaacgaag cacggttaca ggcagccgtc aatgacatgg
     1741 aagacaaaaa agcctgggat gtggaggcaa gggtcaagga aatcctcacc aaacttaaaa
     1801 taggcaattt tgaccaaaag gtcaatcaat tgtccggtgg gcaaaagaag agacttgcct
     1861 tggcaaaggt attgatttcc gaaccggaac tgctcatcct cgatgaaccg accaaccatc
     1921 tcgatatgga gatgatcgaa tggctggaag aatatctgca acaccctggc cttactctcc
     1981 tactggttac acacgaccga tattttctgg aaaatgtctg catccagatc cttgaactgg
     2041 aaaatggtaa tctgtataaa tattcgggaa actattcgga ttatctggaa aagaagcacg
     2101 ccaaggcaga aaatgaagct atcgtccttg ataaaaacaa gaaactgttt cttaaagagc
     2161 ttgaatgggt gcgtcggatg ccacaagcca gaacgaccaa ggcaaaagcg aggatagaca
     2221 agtttgatga gttgaaaaat gaactttcgc aacagcgcga agccgccata ctccagatca
     2281 atatcaagtc caatcgcctc ggttccaaga tcatcgaagc gcacaatgta ggcaaatcct
     2341 acaatgacag gcatcttttt tcgcacttca attataaatt caaccgtttt gaccgtgtag
     2401 gcatcattgg gccaaacgga tccggtaaat ccacattcct gaatatcttg acgggcaatc
     2461 tgaaacccga cgagggaaaa atcgtaatag gagataccgt cgtatttggc tactataccc
     2521 aatccggaat caacctgaag gaagacaagc gcgtgatcga cgtcatcacg gatgtagctg
     2581 aatacatacc caccgaaaaa ggccataata tcacagctgc atccctgctg gaaagattca
     2641 tgtttccccg ggaacaacaa caagtgtacg tatcccagct gagtggtgga gaaaagcgc
//
LOCUS       DAOQMC010000290         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22507_length_2691_cov_4.376707, whole genome shotgun sequence.
ACCESSION   DAOQMC010000290 DAOQMC010000000
VERSION     DAOQMC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22507_length_2691_cov_4.376707"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PKV31_09495"
     CDS             complement(<1..1037)
                     /locus_tag="PKV31_09495"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNB93133.1"
                     /translation="MSKRIFFVLMLLTFFNSCIFGQLSEDKRMIRQLHELSVTKGKAY
                     DWLEFLCKDIGHRLSGSPQLAAATNYTYYLMKELGFDSVWLQSCKVPHWERGPKEIVR
                     VTGSVEGQFDLSALALGNTWGTGPNGVSGEVIAVYGLEQLSKMPDSLVKGKIVFFNRP
                     FAEAPLNTFESYGMTVDQRGGGPALAAKKGAIAALVRSVTSSNDDIPHTGGSWFGEEG
                     PFIAAYAIGCQSADRLAHALKLGKVVAYCQSDSRMLPDADGWNVIGEIRGSKYPEEII
                     VVGGHLDSWDVGEGAHDDGAGTVQSIEVLQLLRLAGYKPQRTIRAILFTNEENGARGG
                     KAYKTFTDKTN"
     gene            1636..>2691
                     /locus_tag="PKV31_09500"
     CDS             1636..>2691
                     /locus_tag="PKV31_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB93134.1"
                     /translation="MSHFETKALRAGYNPANSAGSSSVPIFQTVSYVFEDTDYAAGVF
                     NLSRPGYIYTRLNNPTNDILEQRIAALEGGIGAVVTSSGTSAIATTLLTLLRSGDHIV
                     ASESLYGGTFNLLNVTLPRFGITSEFVDPSAPDNFSRAIRENTRAIFIESLGNPKLDV
                     LDIKAIAAVAKAHRIPLIVDNTVATPYLLRPLEHGANIVIHSLTKYAAGNGTTLAGAI
                     IDGGTFDWSNGKYPEFTEPSPGYHGLIYHDVLGASAFIAKIRIEGLRDLGAALSPFNA
                     FQVIQGLETLPVRVSKHSENALNLARWLEEQDEVAWVNYPGLPSSNYYDLAKQYLPNG
                     QSGLLTIGLKGGYEAAKI"
BASE COUNT          812 a          605 c          537 g          737 t
ORIGIN      
        1 tcatttgtct tatcggtaaa tgttttgtaa gctttacccc ctctagctcc attctcttca
       61 ttcgtaaaaa gaatggcgcg aattgtccgc tggggcttgt atccggccaa tctcagcaat
      121 tgcaggactt caatagactg gacagtacct gcaccatcat catgagcacc ttctccaacg
      181 tcccaactat ccaggtgtcc acctacaacg atgatttctt ccgggtattt actacctctg
      241 atctcgccga ttacattcca gccgtctgca tccggaagca tcctgctatc tgattgacaa
      301 taggcaacga ctttgccgag cttcaatgca tgagccaggc gatccgctga ttgacagccg
      361 atagcatagg cagcgatgaa aggtccctcc tcgccaaacc agctaccccc ggtatgtgga
      421 atatcatcat tggaactcgt cacactccgc accaatgcgg caatggcgcc cttttttgct
      481 gcaagtgctg gaccgccccc tcgctgatct actgtcatgc cataggattc aaaggtattg
      541 agtggcgctt ctgcaaaggg tcggttaaag aatacgattt tacctttaac caatgaatca
      601 ggcatctttg aaagctgctc aagcccatac acagctatta cttctccact aaccccattc
      661 ggaccggtgc cccaagtgtt gcccaaagcc agcgctgaaa ggtcgaattg tccttcaaca
      721 gaaccagtga cccttacgat ctccttaggg ccacgctccc agtgtggcac tttacaagat
      781 tgaagccaaa cagaatcaaa gccaagttct ttcatgagat agtaggtata gttggtcgca
      841 gcggctaatt gcgggctacc acttagcctg tgaccaatat ccttacagag gaactcaagc
      901 cagtcatatg ctttgccttt ggtaactgac agctcatgca attggcgaat catcctctta
      961 tcttctgaaa gttgaccaaa aatgcatgaa ttgaaaaaag tgagaagcat aagaacgaaa
     1021 aaaatccttt tactcatacc gaataattat ttacttagtt atatatacaa aagtaataaa
     1081 acaggaatcc aaacaaataa ttattgaaac cattcatatg acaaaatgaa tactgaacat
     1141 ttttatctta actttatatt attgattata tgttatatac atattatata ataaaatatt
     1201 taaacaaaca ttacctcatt ttaaaaaata atttctcatc tttgctgcac gaaaaagaaa
     1261 tcaatgaatc agattaataa caatatgcag gccatgatga tgtgtatagg tacacgaggc
     1321 ttgttatttg tataatctga atataaaaag ataaatatat aatgagcctc tgcagatgca
     1381 ggggcttttt tttgacatta tgaaaaaaat aaaattcgaa agatatcggc tgatagtcac
     1441 tacatctagt gaccaaggag caatgtgttg ttgtatgatg tgcatgatga tgcgccacaa
     1501 agcccgttgc cttaagtcac gagattagag atccaaagaa aatctgtaag aagtcctttc
     1561 ggcaataacc ggaaggactt ttttttaacc ttaaattatc attgtaaaca ttgtaaacat
     1621 ttaaaatttt taaaaatgag tcattttgaa accaaagcat taagagccgg ttataatccg
     1681 gcaaattcag caggcagtag ttctgttcca attttccaga ctgtatcgta tgtatttgaa
     1741 gatacggact atgcggcagg cgtctttaac ctttcaagac cgggatacat atacacccgt
     1801 ctaaacaacc cgaccaatga catcctggaa caaaggattg cagctttgga gggtggcata
     1861 ggcgctgttg tgacttcatc gggcacatct gccatcgcca ctaccctgct tacgctactc
     1921 agatcaggcg atcatatcgt agcttccgaa agtctatatg gaggcacttt caatctactg
     1981 aatgttactc tacccaggtt tggcattaca agcgaattcg tcgacccttc agctcctgat
     2041 aatttcagca gggcaatccg tgaaaatacc agggctatct tcattgagag tcttggaaat
     2101 cccaagcttg atgtgttgga catcaaagct atagcagcag tagctaaggc tcatagaata
     2161 ccgttaattg tagacaacac tgtagcaact ccttacttac taaggccatt agagcatggt
     2221 gccaatatcg tcattcactc actcacaaaa tatgcggcgg gcaatggaac cactttagca
     2281 ggggccatta ttgatggcgg aaccttcgat tggagtaatg gtaaatatcc agaatttacg
     2341 gagccatcac caggatacca cggtttgata tatcatgatg tattaggcgc ttcagctttc
     2401 atcgcaaaaa ttaggattga gggtctcaga gaccttggtg cagccttaag tccattcaac
     2461 gctttccagg ttatccaagg gctggaaacc ttgcctgtca gggtcagcaa acatagcgaa
     2521 aacgcactta atctcgctcg atggcttgaa gaacaggacg aagtcgcatg ggtcaactat
     2581 cctggtctgc catcaagtaa ctactatgat ctggcaaaac agtatctacc taatggtcag
     2641 agcggtttgc taacaattgg tctgaagggt ggttatgaag cggctaaaat c
//
LOCUS       DAOQMC010000291         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22743_length_2676_cov_5.447921, whole genome shotgun sequence.
ACCESSION   DAOQMC010000291 DAOQMC010000000
VERSION     DAOQMC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22743_length_2676_cov_5.447921"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..803
                     /locus_tag="PKV31_09505"
     CDS             171..803
                     /locus_tag="PKV31_09505"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNB93135.1"
                     /translation="MDITQLLSGGLGQQVINGIGNQTGTSEQETSSVINAALPVLMGM
                     LRKNASTEEGESGILNALNKHNGGIFDHLSDFLGSGDTSDGNGILGHILGNKKSSVED
                     QISQQTGVSSLNVSKIIAMLAPIVMGMLGKQANQSQTSNAGGLGNILGGLLGAGGASS
                     SSVGGGILASILDQDGDGQLGVGDLLSAVSGPDKKSGGLGGLLGGLFGKQ"
     gene            complement(901..1398)
                     /locus_tag="PKV31_09510"
     CDS             complement(901..1398)
                     /locus_tag="PKV31_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB93136.1"
                     /translation="MSLKQALIDEIEREAVSTGKMLERIPEDKFDWSPHARSMAMKAL
                     AVHIVGIAAYTDVIATTDYLDIGERRPPQPEINTTQDIVDAFESATQKSLDALHTLSE
                     EALGEEWIMRFGERIILRASKATAMRTMAMNHMYHHRAQLGVYLRLLDIPVPGMYGPS
                     KDERL"
     gene            complement(1425..2030)
                     /locus_tag="PKV31_09515"
     CDS             complement(1425..2030)
                     /locus_tag="PKV31_09515"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB93137.1"
                     /translation="MDREKNTKNGLDVAFWNQRWLDGTTGWDIGYASPPITDFLSGYD
                     QRDARILIPGCGNAYEAEFLSDNGFSDIVLLDIAPEAVRRLKEKFTLSKAVTVVCGDF
                     FEHEGKYDLILEQTFFCAQVLERREEYVEKVHTLLNPGGILAGVLFNIDFEQAGPPFG
                     GDIEEYRRLFEPYFNIAIMQPCYNSIAPRAGSELFVKMIKK"
     gene            complement(2245..>2676)
                     /locus_tag="PKV31_09520"
     CDS             complement(2245..>2676)
                     /locus_tag="PKV31_09520"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNB93138.1"
                     /translation="VLSAILGLGPGFIMIYVVTRASTMTELRHAYLIRNRLHIFVMIG
                     GTILLLTGLAMGILNPYWFRQGWYITSLTLFLIALGFGPAVLSPLSKPIKKLLKEHQG
                     DDIPVHYNELIRKLFFYERIENVLFIIIIVLMVLKPDFQLW"
BASE COUNT          810 a          575 c          540 g          751 t
ORIGIN      
        1 acatctaaac ttataaatat gttgtttttc atacacaccg ggaaatggat tgattataaa
       61 ttttcaatca aatggtaata aatggtaata aatggtaata aaagcttaat gtactaaaat
      121 aatgaattaa taatcacaac tgattgttta caccaaaaat caatcaagaa atggacatta
      181 cacaactttt gagcggtggt ctcggacaac aggtcatcaa tggtatcggc aatcaaaccg
      241 gcacctctga acaagaaact tcttcagtta ttaatgcagc tctgcctgta ttgatgggca
      301 tgttaaggaa aaacgcatct accgaagaag gagagtcggg catcctcaat gcactaaaca
      361 agcacaacgg aggcatattc gaccatttgt cagacttttt gggatccggt gacacatcag
      421 acggaaacgg tattctggga cacatattag gtaacaaaaa aagcagtgtt gaagatcaga
      481 taagccaaca aacaggtgtg tcttccctca atgtgtctaa gattattgcc atgcttgcgc
      541 cgatcgttat gggaatgctg ggtaagcagg ctaatcaatc tcaaaccagc aacgccggtg
      601 gacttggcaa tatattaggt ggactattag gggcaggagg agcatcctca tcatctgtag
      661 gtggcggcat cctcgcttct atcctggatc aggacggaga tggccaatta ggcgttggcg
      721 acctgctaag tgcagtttcg ggaccggata aaaaatcggg tggccttgga ggcttattag
      781 gtggattgtt cggaaagcaa taattatcac ctgattgggt gaaggtacaa acatgaatcg
      841 ggtgagaaaa ttggaaaagt aatccatttt ttctcacccg attggtttta ataagaaatg
      901 tcaaagcctt tcatccttgc tgggtccgta catgccgggt accggaatat caagtaagcg
      961 aagatatact cctaattggg cacgatggtg atacatatga ttcatagcca ttgttctcat
     1021 cgctgttgct ttagatgccc gcaggatgat acgctctccg aatctcatta tccattcttc
     1081 ccctaatgct tcttcgctga gcgtatgcag ggcgtctaat gatttttggg tagctgattc
     1141 aaacgcgtcg acaatatcct gagtcgtgtt aatttctggt tgtggcggcc ttctttcccc
     1201 aatatccaga taatcggtag ttgcaattac atctgtatat gctgctattc ccacaatatg
     1261 cacggcaaga gctttcatag ccattgacct ggcatgtggc gaccaatcaa atttatcttc
     1321 cggaattcgc tcaagcattt tcccggtact tactgcctct cgttcaattt cgtcaataag
     1381 tgcttgtttt aatgacatct aaatggtttt tgttgttaaa taaattattt ctttatcatt
     1441 ttgacgaaca attcagaacc ggctctgggc gctatactat tgtagcatgg ctgcatgatc
     1501 gcaatattga aatacggttc gaacagtctt ctgtattcct ctatatcacc tccaaatggc
     1561 ggtcccgctt gctcaaaatc gatattaaag agaactcctg ccaggatacc gccgggattg
     1621 agcaatgtat gaactttctc cacatattcc tcccttcttt caagtacctg tgcacaaaag
     1681 aaagtttgct caaggatcag gtcgtactta ccctcatgtt cgaaaaagtc accacacacc
     1741 acagtcacag ccttcgataa ggtaaatttc tccttcaagc gcctgacggc ctccggcgca
     1801 atatccagta atacgatatc agaaaatcca ttgtcagaaa gaaattctgc ttcataggca
     1861 ttaccacagc caggaattaa gattcgggca tctctttggt cataccctga taaaaagtcg
     1921 gtaataggcg gagatgcata accgatatcc cagccggtgg ttccgtcaag ccatcgttga
     1981 ttccagaacg caacgtccag accattctta gtgttttttt ctctatccat taaaaaagat
     2041 ttaactggtc ataattaggc atcctttcaa atgctgcatg ttaatttcaa agggaaatat
     2101 taaattattt tcccctttag aacaaaatat aattaatacc gaaggtacat tcgttattat
     2161 accaaaaatt tatggactat tatataaatc atcctggtat aacatcacaa gctacgaaaa
     2221 gattaagaaa gacaacttgc atgctcacca aagttgaaag tccggcttca gcaccattaa
     2281 tacgataatg ataataaata gaacattttc aatccgctca taaaagaaca gctttctgat
     2341 cagctcatta tagtggactg gaatatcatc accctgatgc tccttcaaca gtttctttat
     2401 gggtttggaa agaggcgata acactgccgg tccaaatccc aaagctatca gaaacagggt
     2461 taggctggtg atgtaccacc cctggcgaaa ccaataagga ttgagaatcc ccattgctaa
     2521 cccggtcagt aaaagaatcg taccaccaat cataacaaaa atatgtagtc ggtttcgaat
     2581 gaggtaagca tgccttagtt ctgtcatagt actcgctctt gtgaccacat atatcatgat
     2641 gaatcccggc ccgaggccga gaatggccga aagaac
//
LOCUS       DAOQMC010000292         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22780_length_2674_cov_3.937763, whole genome shotgun sequence.
ACCESSION   DAOQMC010000292 DAOQMC010000000
VERSION     DAOQMC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22780_length_2674_cov_3.937763"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="PKV31_09525"
     CDS             <1..2073
                     /locus_tag="PKV31_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93139.1"
                     /translation="TTFEVKSDLFADGDQRLINHKKYYYTAVAYAYNNYKKMDYKGDG
                     QRITFALGRRNSQIYTAIPRPIVDRVLNSDYGSGAVITRIDGEGNYRSFLELADGERD
                     RLFNLSFENGSSPERTIQYKEGFGPVAVKVYNPIDVENGVMNVAFNDANPTDGKLDIN
                     SAVWHLTNSAGDTLVNAEKFSKFNETLIAEFGISVFFPNNEPGEDRDDVNGYIGQRLQ
                     YSDATKDNWLSMLRDGGEGVTENPNISLILSEALNWINYVEKFSGNPDPKGVYEKLGF
                     WPLTLALKEPQDVAVNEFLQPVPPSSNIASTVSKSNTLADLNNVDIVFTNDRSKWSKC
                     IVVEASNTKYSGEGLPPVGSAPQFSLRKSRSITKDFDADGNPVLNANPDSTGMSWFPG
                     YAIDVETGMRLNIFFAENSSYDPAIFGDLIQEVGDNPNCGQDMIFNPGGNLFINASNN
                     LLSAANFFAGGQHYIYVTKTKYNECATFLNNLKGQNPSDINKSRVLAQVTWVGIPLGF
                     NMLSFKDGLIPQETVYKLRVNNSYKVPALADGRTGVNNGMPMYKIEIKDKAADTKSPT
                     IAPSALDKISVVPNPYYAYSSYENTEISNIVKITNLPPKCVVTIYSLDGKFIRQFNRN
                     EVRREVSGEARGVRYAQVTPDIEWDLKNGSGITVGSGIYLIHIKSDDGERVIKWVGAI
                     RQLEISGF"
     gene            2146..>2674
                     /locus_tag="PKV31_09530"
     CDS             2146..>2674
                     /locus_tag="PKV31_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93140.1"
                     /translation="MKRLFTIICLIAFSQIGFAGNPDRQGSAGAGELLINPWARSAGL
                     HTMNTAGISGIEATRLNIAGLASVKSMEIGYSHGRYLVGTGISTNALGLAKKIGKKGV
                     FGLDFMSVNFGDIPVTTVNFPEGNGANFNVAWTNIGLSYAHNFFNEESLGKVSVGLGV
                     RLVMESIPGISATGVA"
BASE COUNT          800 a          458 c          617 g          799 t
ORIGIN      
        1 actacttttg aagtgaaatc tgacttgttc gccgatggag atcagcgctt gatcaaccat
       61 aagaaatatt attacaccgc tgtagcctat gcctataata attataaaaa aatggattac
      121 aaaggtgatg gacagaggat tacatttgca ttgggaagac gtaactctca aatatatacg
      181 gcaattccac ggcctattgt agatcgtgtg ctcaattcag attatgggtc aggcgccgtt
      241 ataaccagaa ttgatggtga gggcaactat agatctttcc ttgaattggc ggatggcgaa
      301 cgtgatagac tgtttaacct cagctttgaa aatgggtctt cacctgaaag aacgatccaa
      361 tataaagaag gttttggtcc ggttgcagtt aaagtttata atccgataga tgtcgaaaat
      421 ggtgtaatga atgttgcatt taacgatgct aacccaactg atggtaaact cgacattaat
      481 tctgcagttt ggcatttgac caattctgcc ggagatacac tggtaaatgc ggagaaattc
      541 tctaaattta atgaaacatt gattgcagaa ttcgggatat ccgttttctt ccctaataat
      601 gaaccgggag aggaccgtga cgatgtgaat ggttatatag gtcaaagatt gcaatacagt
      661 gatgcgacta aggataactg gctttctatg ttaagagatg ggggagaagg ggttacagaa
      721 aatcctaata tttccctgat cttgtccgaa gcattgaatt ggattaatta cgttgagaaa
      781 tttagtggta atccggatcc taaaggagta tatgaaaaac ttggtttttg gccactgact
      841 ttggcgttaa aagagcctca ggatgtggca gtcaatgagt ttcttcaacc ggtcccacct
      901 tcatccaata ttgcatccac agtatctaaa tcaaatactt tggcagattt gaataatgtc
      961 gatattgtat ttaccaatga tagatcaaaa tggtcaaagt gtattgtggt cgaggcgtcc
     1021 aatactaagt actcaggaga aggccttcca cctgttggta gtgcacctca gtttagcctt
     1081 cgtaagtcac ggtccataac taaggatttt gatgccgatg gcaatcctgt tttaaatgcc
     1141 aatcctgact ccacaggtat gagttggttc cctggttatg caattgatgt agagacagga
     1201 atgaggctta atattttctt tgcagagaac tccagttatg atcctgctat tttcggtgat
     1261 ctgattcagg aggtcggcga taaccctaat tgtggtcagg atatgatttt caatccgggt
     1321 ggaaatttat ttattaatgc tagtaacaac ttactaagtg cagctaattt ctttgcaggg
     1381 ggtcagcatt atatatacgt cacaaagact aaatacaacg aatgtgctac ttttctgaac
     1441 aatttgaaag gtcagaatcc ttccgatatt aataagtcca gggttcttgc tcaggtgacc
     1501 tgggtgggca tacctcttgg ttttaatatg ttgtcattta aagatggatt gatccctcag
     1561 gagactgtct ataagcttcg tgtcaataat agttataaag ttcctgcatt ggcagatggt
     1621 agaacaggtg tcaataatgg tatgccgatg tataagatag aaatcaaaga taaggctgcc
     1681 gacacaaaat caccgactat tgctccaagt gctttggaca agatatcggt tgttccaaat
     1741 ccttattatg cctattcatc ctatgaaaat acggaaatat caaatattgt taaaataact
     1801 aatctgccgc ccaaatgtgt ggtaaccatt tattcccttg acggcaagtt tattcgacag
     1861 tttaacagga atgaggtcag aagggaagtg agtggtgaag caaggggtgt gcgatatgca
     1921 caggtgacgc ctgacattga atgggatttg aagaacggta gtggtattac tgttggtagt
     1981 ggtatttatc taatccatat caaatctgat gatggagaaa gggtaatcaa gtgggttggg
     2041 gcaatccgtc agcttgaaat atcaggattc taatgatata aatggttccg gagttatccg
     2101 gaaccattaa aacgaacatt ttttaggacc aaaaaagaat ttaaagtgaa aagattattt
     2161 acgataatat gcttaatagc attctcacaa attggttttg ccggtaatcc cgatcgtcag
     2221 ggttcagctg gcgccggtga gcttttaatc aacccatggg ctagaagtgc cggactgcat
     2281 accatgaata ctgcaggaat cagcggtatt gaagcaaccc gtcttaatat agccggatta
     2341 gccagtgtta aaagtatgga gattggttat agtcatggtc gatatttggt tggaacaggt
     2401 ataagcacaa atgcattggg actggctaag aaaattggga aaaaaggggt tttcggcctt
     2461 gatttcatgt cagtcaactt tggagatatc cctgtaacta cagtcaattt cccggaaggc
     2521 aatggggcta atttcaatgt cgcatggact aatatcggat tatcttatgc acataatttc
     2581 tttaatgagg agtcattagg taaggtttct gtaggtctgg gtgtacgtct tgttatggaa
     2641 tcaatccctg gcatatctgc taccggagtc gcgt
//
LOCUS       DAOQMC010000293         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22885_length_2668_cov_3.376196, whole genome shotgun sequence.
ACCESSION   DAOQMC010000293 DAOQMC010000000
VERSION     DAOQMC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22885_length_2668_cov_3.376196"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2273)
                     /gene="polA"
                     /locus_tag="PKV31_09535"
     CDS             complement(<1..2273)
                     /gene="polA"
                     /locus_tag="PKV31_09535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNB93141.1"
                     /translation="MSSDKRIFFIDGHAMVYRAHYAFINRPLMNSKGQNVSAINGFIR
                     AIWDLIVNQKPTHLGVVFDPKGGTFRSDLYEEYKAQRDAQPEDISFAIPYIIKILEGM
                     NIPVEIIPNYEADDVIGTLAKKAEKEGFEVYMVTPDKDYAQLVSENIFQYKPARFGND
                     IEILGVEEVLKKWEIKRVDQVIDILGLMGDSVDNIPGLPGVGPKTAVKLIEEFDNLEN
                     VLANADKMKGKLQETVQTKADLARLSKTLATIHIDAPVDFDYNHYEVNEFNNDVLLEV
                     FKELEFKTLAATILSYNSKTIGKSTSTTKAASTKKQDSEVYQGSLFDNKDTVEEKETL
                     SEELSNAQFNITNTPHEYKLVATDDDWKALRTEITARKFFCFDTETTSLDPIVAELVG
                     ISISTEAHKAWYISVPEDQQVAQVIANQLKPLWENPEIDKIGQNLKYDISVLKKYGID
                     IKGRLFDTMLIHYLIEPELRHGMDYMAEAYLKYRPIPITELIGEKRERQLSMRDVALE
                     QIKEYASEDADITLQLYNYLVPGLKEIQAYDLYNNIEGKLITVLADLEMAGVNIDAEY
                     LNHYSVELENRIVELERKIYQEAGSEFNINSPRQVGDLLFGTMKIPYRWAKTKQGQHS
                     TDEAKLSELALEYAFVQDILEHRSLSKLKSTYVDALPKMVHHSTGKVHSSFNQALAAT
                     GRLSSNNPNLQNIPIRTREGREIRKAFIPSGQDNILLSADYSQIELRIIAELSKDKNM
                     VEAFQLGHDIHTATASKV"
BASE COUNT          669 a          540 c          523 g          936 t
ORIGIN      
        1 acttttgaag cagtcgcagt atgtatatcg tgtccaagct ggaatgcctc taccatattt
       61 ttatccttgc tgagctctgc tatgatgcga agttcgattt gagaataatc tgcagaaagg
      121 agtatgttgt cctggccgga aggaatgaac gcctttctga tttcccggcc ttcccgagta
      181 cggatcggta tgttctgcaa gttcggattg ttggaactta gtcttcctgt tgcagcaagt
      241 gcctgattaa aactgctgtg aacctttcct gttgaatgat gaaccatctt agggagtgcg
      301 tcgacatagg tagacttgag cttgcttaag ctgcggtgct ccagaatatc ctgcacaaat
      361 gcatactcta atgctaattc ggataatttg gcttcatctg ttgaatgttg accttgtttg
      421 gtttttgccc accggtaagg aattttcatg gtgccgaaca atagatcacc aacctgtcgc
      481 ggactattaa tgttaaattc tgaccctgct tcctggtaaa tctttcgttc cagttccaca
      541 atcctgtttt ccaattccac actgtaatgg ttaagatatt ctgcatcgat gtttacaccg
      601 gccatttcca gatcggccaa aaccgttatc agcttgccct caatgttgtt ataaagatcg
      661 tatgcttgaa tttccttaag gcctggtaca agataattat ataactgtaa ggtaatatcg
      721 gcatcctcag aggcatactc ctttatttgt tccaaagcca cgtcccgcat ggatagttgt
      781 ctttctcttt tttctccgat caattcagta attggtatcg gtctgtattt aagatatgct
      841 tctgccatat agtccatgcc gtgccttagt tctggctcga tcaggtagtg aataagcatg
      901 gtatcgaata gtcttccttt gatgtcgatt ccgtattttt tcaggacact gatatcgtat
      961 ttgagatttt gaccgatttt atcaatttcg gggttttccc acagcggttt cagttgattt
     1021 gctataacct gagcgacttg ttggtcttca ggcaccgata tataccatgc cttatgagct
     1081 tcagtgctga ttgagatacc cactaattca gcaactattg gatcaagact tgtggtttcc
     1141 gtatcgaaac aaaagaattt ccttgcggta atttccgtcc ttaatgcctt ccagtcatca
     1201 tctgttgcga ccaacttgta ttcatgtggt gtattggtaa tattaaattg ggcattggat
     1261 aattcttccg atagcgtttc cttttcctca actgtatctt tattgtcaaa aagggaccct
     1321 tgatatactt ctgaatcttg tttttttgtg gaagctgctt tggttgttga ggttgacttt
     1381 cctatggttt tagaattata ggataatatg gtggctgcca gggttttaaa ctccagttcc
     1441 ttaaagacct ctaacaatac atcattatta aattcattca cttcatagtg gttgtagtcg
     1501 aaatctaccg gtgcgtcaat gtgaattgtt gccagtgtct tggatagtcg agccaggtct
     1561 gccttggttt gcacagtttc ctgaagtttc cccttcatct tgtcggcatt ggccagtacg
     1621 ttctccaggt tgtcaaattc ttcgatcaat ttgacggcgg tcttcggacc aacacctggg
     1681 aggcctggaa tattatctac actatctccc atcaacccaa ggatatctat tacctggtca
     1741 acacgtttga tttcccattt ttttaacacc tcctctacac ccaggatttc tatgtcatta
     1801 ccgaatcggg ccggcttata ttggaaaata ttttcggaca caagttgcgc gtaatccttg
     1861 tcaggcgtga ccatatagac ctcaaatcct tctttttctg cttttttggc caatgttccg
     1921 atgacatcat ccgcttcata atttggaata atttccaccg gaatattcat tccttcaagg
     1981 atctttatga tgtatggaat agcaaatgat atatcttctg gctgggcatc tctttgagct
     2041 ttgtactcct catataagtc gcttctgaat gtgccgcctt ttggatcaaa tacaacaccc
     2101 agatgcgttg gtttttggtt gacaatcaaa tcccagatag ctcttatgaa tccattgatt
     2161 gcagagacat tttgcccttt ggaattcatc agtggcctgt tgatgaatgc gtaatgggct
     2221 ctgtatacca tcgcatggcc atcaatgaaa aaaatcctct tgtctgaaga cataacttta
     2281 attttatggg taaaagtaag gtattgtgcg gggcttacat taaattttga acaatttatt
     2341 attcgtttgg gaggttttgg aacagcattt aaaggtatcc cgttctgatt tttatttttt
     2401 tcatattgat tttcaaaaag aatgaaaata aaatatttat ccttttggta tgggacctac
     2461 agtagggtag actttacttt tatgattgtt aaaatttttt tatatccggc agtatttcca
     2521 tcaatgaact ttcctgttag gaatttgttt agcaatacga ttttttctta atttagaaaa
     2581 gatataaata aaccgcaaat ctttgtttcc acaaatggct aaagtaactt ttaaatttga
     2641 agataagcag cctgacgtca ctattgag
//
LOCUS       DAOQMC010000294         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_22895_length_2667_cov_4.692956, whole genome shotgun sequence.
ACCESSION   DAOQMC010000294 DAOQMC010000000
VERSION     DAOQMC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22895_length_2667_cov_4.692956"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKV31_09540"
     CDS             complement(<1..613)
                     /locus_tag="PKV31_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNB93142.1"
                     /translation="MKRRSFIINSGLMLGTGGLDMTAFQQEGPVKGKPIVISTWNFGL
                     EANKEAWRILSANGNALDAVEKGVMVAESDLNNTSVGRGGNPDRDGIVTLDASIMDQH
                     GNAGSVAALEDIEHPISVARLVMEKTPHVMLAGDGARQFALENGFKPIDLLTEKSKAN
                     YKKWLETSKYKPVINIENHDTIGMLALDASGNFSGACTTSGLAF"
     gene            complement(637..>2667)
                     /locus_tag="PKV31_09545"
     CDS             complement(637..>2667)
                     /locus_tag="PKV31_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB93143.1"
                     /translation="ISFPMKILTVPILILLTMAASTVLAQPLKTIPYQARIEKADEYA
                     DRNNYASQLDELEEVYKERKDKSMLPLMAQLNYKLRDYGRAENLYKRIVDGDKKGTDI
                     EARYMYGKMLKLNGKYQEAAEVLREVKNTDDDVYAPLAIMELKGILLAVSTDTVKQIS
                     VVPAGPKINTKSGEYSPTLYNNELYFTSTALAAGEEPGNTYVVKLLKSQRDKDGGWSK
                     ASEVDALISASSPAVGNVAFSQADQIMYLTKVTFDGEEVATSRVYYSKREGDAWTDPA
                     LVKGLPDSFIVKNPMPGELYGRDVLFFSSNAPGGRGGFDIFYATKISEGIFDSPVNLG
                     PTVNTAGDEITPFYKEGKLVYATDGLPSYGGFDLYSTEWNGTSWSTPANMGPGFNSPA
                     DDMYYTIDEEGYEGLLVSNRVGTTSLRGKTCCDDIFTFSIAKPVVELEVYVLDENKPL
                     RHGELSIMEQFKPETKVTEGKDDTYHFSYELDINKGYFIKVTRIGFFPDSLYVKTTGI
                     KEDSTMIVKINLKPKPEVEVISINQPIRLNNIYYNYNDAKILKAAMPDLDYLYELMQQ
                     YPDMVIELSSHTDSRGNDEFNMKLSQRRADSAMNYLLAKGIAPERIQAVGYGESMLLN
                     NCGNDVKCSEEEHQLNRRTEFKIISGPTNIEIKKQITRERGKIIDTKTIKEK"
BASE COUNT          672 a          635 c          552 g          808 t
ORIGIN      
        1 taaatgccaa accgcttgtt gtacaagcgc ctgaaaaatt tcccgaagca tcaagggcaa
       61 gcatgccgat ggtgtcgtga ttttcgatat tgatcacggg tttatatttg gatgtttcca
      121 accatttctt ataattggct ttactttttt ctgtcagcag atcaatgggt ttgaagccat
      181 tttcaagggc aaactggcgt gcgccgtcac cggctagcat gacatggggt gttttctcca
      241 tgaccaatct agccactgat atcgggtgtt cgatgtcttc aagggctgct acagaaccgg
      301 cattaccatg ttgatccatg atgcttgcat caagggtcac gattccatcc cggtcgggat
      361 tgccgccacg tcctaccgaa gtattgttga ggtcagattc ggctaccatc acccccttct
      421 ccactgcatc gagggcattg ccattggctg acaggatacg ccatgcttcc ttgttggctt
      481 caagaccgaa attccaggta gagataacaa tcggctttcc tttgacaggg ccctcctgtt
      541 gaaaagctgt catatcaaga ccaccggtgc caagcatcag gcccgaattg atgataaaac
      601 tccttctctt catgacaagc ttttaatatc aaagaattat ttttccttaa tggttttcgt
      661 atcaatgatt tttcctcttt cccgtgtgat ttgtttctta atttcaatat tagtcggtcc
      721 ggaaataatc ttgaattcag tacgcctgtt gagttgatgc tcttcctcac tgcacttgac
      781 atcatttcca cagttgttga gcaacatgga ttccccgtat cccaccgctt gtattctctc
      841 cggagcaatg cctttggcca acagataatt catggcagag tccgctctac gctgggacaa
      901 tttcatattg aattcatcat taccacgcga gtcggtgtgt gaggacaatt cgataaccat
      961 atccggatat tgttgcatca attcgtacaa ataatccaaa tcaggcattg cagccttcag
     1021 aatttttgca tcgttataat tataatagat gttgttgaga cggataggct gattgatgct
     1081 aattacctca acttcaggtt tcggttttag gttgattttg acaatcatgg tactgtcttc
     1141 cttgataccg gttgtcttta cataaagaga atcggggaaa aatccaatcc tggtcacctt
     1201 aataaaataa cctttattga tatccagttc ataagaaaaa tggtaggtat catcctttcc
     1261 ttctgtaact tttgtttcgg gtttaaactg ctccataatg gacaattcac catggcgcaa
     1321 tggtttgttc tcatcgagga catatacttc cagttctacc acaggctttg ctatggagaa
     1381 ggtaaagata tcatcacagc atgttttgcc gcgtaatgag gtagtgccta ccctattgga
     1441 taccagcagg ccttcgtaac cttcttcatc gatggtatag tacatatcat cagccggaga
     1501 attaaaaccc ggacccatat tggcaggagt ggaccaggag gtgccgttcc attctgtgga
     1561 atacaggtcg aaaccaccgt atgaaggaag tccgtctgtt gcatacacca attttccttc
     1621 tttataaaag ggcgtaattt catcgcccgc agtattgaca gttggtccaa gattgaccgg
     1681 tgaatcgaat atgccctcgg aaatcttagt cgcatagaag atatcgaatc ctcctcttcc
     1741 accgggggca ttagaggaga agaacagtac atctctgccg tataattctc ccggcattgg
     1801 attcttgacg ataaaagaat ccggcagacc tttgaccagt gcgggatcag tccatgcatc
     1861 tccttcccgc ttactgtaat acaccctgga tgtagctact tcttcgccat caaaggtaac
     1921 cttggtcaga tacataatct gatcggcttg cgaaaaggcc acattgccca cagccggact
     1981 ggatgcagat atcagtgcat caacctcaga agccttactc catcctccat ccttatccct
     2041 ttgtgacttc agcaatttga ccacataagt atttccgggc tcttcaccgg cagcgagggc
     2101 tgttgaggtg aagtacaatt cattgttgta tagagtgggt gagtattctc cgctttttgt
     2161 attgatcttg ggtcccgcag gcactacgga tatttgcttt acggtatccg tacttactgc
     2221 gaggagaata cccttgagtt ccatgatagc aagaggggca tatacatcat cgtcggtatt
     2281 cttaacctct ctgagcactt ctgcagcttc ctggtatttg ccattgagtt ttagcatttt
     2341 accatacata taccgggctt ctatatccgt acctttcttg tcgccatcca caatccgttt
     2401 atacagattc tccgcacgac catagtctct cagcttataa ttcaattgcg ccatcagagg
     2461 caacatggat ttgtcctttc tttccttata cacctcttcc agttcatcca gttgactggc
     2521 ataattattc ctgtcggcat attcatcagc cttctcaatg cgagcctgat acggaatggt
     2581 cttcaatggc tgggccaaca cagttgaagc tgccattgtc aacaaaatca atataggaac
     2641 ggtcaatatc ttcataggga atgagat
//
LOCUS       DAOQMC010000295         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_23007_length_2661_cov_4.548350, whole genome shotgun sequence.
ACCESSION   DAOQMC010000295 DAOQMC010000000
VERSION     DAOQMC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23007_length_2661_cov_4.548350"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PKV31_09550"
     CDS             <1..1325
                     /locus_tag="PKV31_09550"
                     /inference="COORDINATES: protein motif:HMM:NF002557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB93144.1"
                     /translation="ITMGIHTIMKAKRIILMAWGMKAPIVAKAVEETMTEQIPASLLQ
                     DHPDCRFVIDTLASTELTRIKSPWLAGEITWTPDMTKKAVVNMAIKTGKPILSLTNND
                     YNENGLGDLLVEKGDSYDINLEVFYALRDSITGWPGGKPGNQLPNHPERSHIIPKRCL
                     IFSPHPDDDIISMGGTFMRLHDHGHEVHVAYQTSGNIAVTDEFVTRFLDFAVGFEQMF
                     GIDNARSEQILNESRSFLQNKKPSQSDTEEVRAIKGLIRRGEATATCRYVGLPEGRWH
                     FQNLPFYETGTIVKNPMGEKDIELTMKLLREIKPHQVFAAGDLADPHGTHKVCLDIVL
                     ESLKRIKEAGDEWIKDCWLWLYKGAWQEWDIGEIMMAVPMSPDQVLKKRHGIFIHQSQ
                     KDMVPFQGSDAREFWQRAEERNANTANLYGALGLTRYAAIEAFVRWDY"
     gene            complement(1400..1555)
                     /locus_tag="PKV31_09555"
     CDS             complement(1400..1555)
                     /locus_tag="PKV31_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93145.1"
                     /translation="MKSFVRLFVPKNYYRLTILQVNRLSWHCEAAFTEAGLMSGVDFE
                     TPTSPKD"
     gene            complement(1714..2424)
                     /locus_tag="PKV31_09560"
     CDS             complement(1714..2424)
                     /locus_tag="PKV31_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB93146.1"
                     /translation="MGTNGRKILLVEDDQNFGDVLKSYLEMNDYEVSLGKDGVSGMEL
                     YRGSKYDLAIFDIMMPRKDGLTLAKEIRERDKDLPIIFLTAKNMKEDILHGFRAGADD
                     YITKPFSSEELLLRIQALLKRSAPKSDPEDEVKDFVIGKYKFNFPLRILTYTPDPTDE
                     EKLSPKEAMLLKMFCKRQNNVLERSEALTKIWGEDNYFTARSMDVFITKLRKYLKKDE
                     NIEIVNIHGNGFQLLVKE"
     gene            complement(2457..>2661)
                     /locus_tag="PKV31_09565"
     CDS             complement(2457..>2661)
                     /locus_tag="PKV31_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB93147.1"
                     /translation="KEARKHIFDKFYRATSGNLHDVKGFGLGLSYVKAMITAHKGTVD
                     VKSELGKGSTFTLYFPYKQQIED"
BASE COUNT          749 a          607 c          591 g          714 t
ORIGIN      
        1 ccatcaccat gggtatccat accatcatga aggccaaaag aatcatcctg atggcctggg
       61 gcatgaaagc accgattgtg gcaaaggccg tagaagaaac aatgacggaa caaataccgg
      121 cttccctctt gcaagaccat cctgattgcc gctttgtcat cgatacatta gcctccaccg
      181 aactgaccag aatcaagagt ccatggctgg caggagaaat aacctggaca cccgacatga
      241 ccaaaaaggc tgtggtcaat atggccataa agaccgggaa gccgatccta agccttacca
      301 acaacgatta taatgagaat ggtctaggcg atctgctcgt ggagaaagga gattcttacg
      361 acatcaacct ggaagtattt tacgctctgc gggattccat caccggctgg ccgggaggta
      421 agccgggcaa tcagttgcct aaccatccgg agagaagtca tataatcccc aaacgttgcc
      481 tgatcttctc cccgcatccg gatgacgata tcatcagcat gggaggtacc ttcatgcgac
      541 tgcacgacca tggtcatgaa gtacatgtcg cctatcagac cagtggcaat attgcggtta
      601 cagatgagtt tgtaacccga tttctggatt ttgcggttgg atttgaacag atgttcggta
      661 ttgacaatgc ccgttctgag cagatactca acgaatctcg ttccttcctg cagaacaaga
      721 aacctagcca gtccgatact gaagaggtta gggccatcaa gggtctgatc cgacgcggtg
      781 aagctacggc aacctgtcgt tatgtaggac tgcctgaagg gcgatggcac tttcaaaacc
      841 tgccattcta tgagaccggg accatcgtta aaaacccgat gggcgaaaag gatatcgaac
      901 tcaccatgaa actgttgcgt gagataaagc cgcatcaggt atttgcagcc ggagaccttg
      961 ccgatcccca tggcacgcac aaggtgtgtt tagacatcgt attggagagt ctcaaaagga
     1021 taaaggaagc cggagatgaa tggattaagg actgctggct atggctatac aagggagcat
     1081 ggcaggaatg ggatatcggg gagatcatga tggctgtacc tatgagcccg gatcaggtgc
     1141 tcaaaaaaag gcacggtatc ttcatacatc aatcccaaaa ggatatggtg ccgttccagg
     1201 gcagtgacgc aagagaattt tggcagagag ccgaagagcg aaatgcgaat acggcaaacc
     1261 tttatggtgc tttgggactt acccgatatg ccgccatcga ggcattcgta cgttgggatt
     1321 attgatcaga aaaatgtacc tgtaacaatt tacaccggta gtttctgatc atgctatttg
     1381 gcaccaatct ccgggagaat taatctttcg gggatgtagg agtttcaaaa tcaactccgc
     1441 tcatcagccc ggcttcggta aaagccgctt cacaatgcca cgacaacctg ttgacctgta
     1501 aaatggttaa cctgtaataa tttttaggta caaaaagccg gacaaaactt ttcatttgtc
     1561 caccatttag tgctgaggaa atattcagga ttgagtcatt gtcagaccgc gaaatatcca
     1621 agttattatg gctatttggc tggtgaaagt aaaattccta tccattctta cgaaaaaatg
     1681 tttgactatc ttttttgaat aggcaaataa atactattct ttcacaagca actgaaatcc
     1741 attgccgtgg atgttgacga tttcgatatt ttcatccttt ttcaaatact tacgcaattt
     1801 ggtaataaaa acatccatac tgcgtgctgt gaaatagttg tcttcacccc aaatcttagt
     1861 taacgcttcg gatcgttcaa ggacattatt ctgccgcttg caaaacattt tgagcagcat
     1921 ggcttcctta ggagacagtt tctcttcatc tgttggatcc ggtgtatagg tcaggatacg
     1981 caatggaaag ttaaacttgt atttgcctat gacaaaatcc ttaacctcat cttcaggatc
     2041 ggacttgggt gcgcttctct tgagcaatgc ctggatgcgg agtaataatt cttcagaaga
     2101 aaatggcttg gtgatataat cgtcagcacc agcacgaaat ccatgaagga tgtcttcctt
     2161 catgtttttg gcagtcagaa aaataatcgg caggtcctta tccctttccc ggatttcctt
     2221 tgccagcgtt aagccatcct ttcggggcat cataatatca aaaatagcca aatcatattt
     2281 cgaaccccga tacaactcca ttccggacac tccatccttg cccaggctga cttcataatc
     2341 attcatttcg agatagcttt tcagtacatc cccaaaattt tgatcatctt ccactaatag
     2401 tatttttcgt ccgtttgtgc ccatttatca atgttgtttt ttctttataa cgtatatcag
     2461 tcctcaattt gttgtttgta tggaaaataa agtgtaaaag tactgccttt gcccaactca
     2521 gattttacat ctactgtgcc tttatgtgcg gtgatcatcg ccttaacata gctcaatcca
     2581 aggccgaagc ccttgacatc gtgcaggttg ccagaagtag cccggtaaaa cttgtcaaaa
     2641 atatgcttgc gggcctcctt g
//
LOCUS       DAOQMC010000296         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_23086_length_2656_cov_4.305652, whole genome shotgun sequence.
ACCESSION   DAOQMC010000296 DAOQMC010000000
VERSION     DAOQMC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23086_length_2656_cov_4.305652"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKV31_09570"
     CDS             complement(<1..230)
                     /locus_tag="PKV31_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNB93148.1"
                     /translation="MYPIQLTQPMAKELESIGFQSLQDPEQVKNALDKKEGTTLLVIN
                     SVCGCAAANARPAVRYALENGRNPDHLVTVFAG"
     gene            complement(1325..2071)
                     /locus_tag="PKV31_09575"
     CDS             complement(1325..2071)
                     /locus_tag="PKV31_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNB93149.1"
                     /translation="MSDSLVIIPTFNEKENIENMVRKVFSLPKPFHILVVDDESPDGT
                     AAIVRELQKEFSENLYLIERKGKLGLGTAYITGFRYGLERNYDYIFEMDCDFSHDPED
                     LIRLYQACHKGGYDMAIGSRYVTGGRVENWPWTRVALSYGASLYVRIITWMNIKDPTA
                     GFVCYKHEVLNTLDLDKIKFIGYAFQIEMKYAVLSLGFKIKEVPITFKDRELGTSKIS
                     KGIVKEGFMGVLKMASKRLFTNYRKSGLAV"
     gene            complement(2084..>2656)
                     /locus_tag="PKV31_09580"
     CDS             complement(2084..>2656)
                     /locus_tag="PKV31_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNB93150.1"
                     /translation="LRGMHICVPRNMTQAAGMYNTLMDGMDPALVIECLNGYRLKEKC
                     PVNLDTFSVPLGIPEILHEGEDVTLVTYGSCVRVAQEAIKLLDTMDISVELIDVQTLL
                     PFDINHIIGASLAKTNRLVILDEDVPGGATAYMLQEILESQDGYFSLDAKPLCITAKA
                     HRTPFGSDGDYFTKPNAEDVVEKIIELIKE"
BASE COUNT          749 a          516 c          526 g          865 t
ORIGIN      
        1 cctgcaaaaa cagttaccag atgatccgga ttcctgccgt tttccagggc gtatcgcact
       61 gcaggccttg cattggcagc agcacagcca caaactgaat ttatcacaag cagggtggtg
      121 ccttcttttt tatcaagggc gttttttact tgttccggat cttgaagtga ttgaaagcca
      181 atggattcca attctttggc catgggttga gtcaactgta tcggatacat aatataacgt
      241 tatttaattt gatttttata aataatgaat aaaacaagaa ggagcgaaat tggttcagta
      301 tttcctgact tgtattgaat tttatgcgat tctattcgta ttttatattt taaatgttaa
      361 gcacatgtta acgatcttaa cggtgttgga tttctcgttt ttaatagaag cttttaaaag
      421 cctcaatcta taagcctttg agcgatactt tccgaaatta ggaagtaaat tttttcattt
      481 tttctcagta gattttgaat ggttactttg ggtccggaat tggatattta aaatgcatat
      541 ttcacattta ttatgattgc tttagttgta aaaattataa aatacagata atcagatata
      601 tatgatatcg caaaatacat attacataaa tatatatata acaaacgtaa aaaaatgtat
      661 ttgtacgagg ctgattattg cctcatcttt gtattgaata tttactgaaa gaagtagcta
      721 ggtaaaagtt cttctaaagt aatgaagaat gtaaaccaat ggtaaaagaa tcatttgggt
      781 ttagatttgg atatgttcat gggattataa ttgttgagag agggtcgtaa atcggccgat
      841 ttacgacccg aactttaact cccgcttagt tttgatttaa gaaatattag gtgtcttgag
      901 catttagttt tggataaaat ttggatttcg tttggatgta agtcttagtg ccgcaatact
      961 aagacttatt tttttgcctt acccttcgga tcagtttaca gatttactca tgtttcgatt
     1021 aaagtgtcta atcaccccat agaggatcaa tttttcgttg cggctcctgt tcaacaactc
     1081 ttcaagtgag ccatggaata ttggttcctc ctgatgagta tgaacttaga tcaaattgtg
     1141 agcatggatc tcgaaagcag attgtgtacc ggtcttttgt aagtttcctt aggtaggtcg
     1201 gagcgttgtg ttcgtcaagg tatatgacaa tcgtgcgttt gggcaaattt gcaggttatg
     1261 gtaaatggag gaatcaaaaa tttattttta agtatattct accctgatgg taaggatttt
     1321 tacgtcaaac agccagccct gatttacgat aattagtaaa gagccttttg cttgccattt
     1381 tcaaaacacc cataaaaccc tccttgacga tgcccttcga gatctttgat gtgcctaatt
     1441 cacgatcttt gaatgtaata gggacctctt tgattttgaa tccaagtgaa agaacggcat
     1501 atttcatttc tatctggaag gcatagccga taaattttat cttgtccaga tctaatgtat
     1561 taagtacttc atgcttgtag cagacaaatc cggctgtggg gtccttgata ttcatccagg
     1621 tgatgattct gacatacaat gatgcgccat aggacaacgc caccctggtc cacggccagt
     1681 tttcaaccct acctcctgtg acataccttg aaccgatggc catgtcgtaa cctcccttat
     1741 gacaagcctg atacaaccgg atcaaatcct cgggatcatg cgaaaaatca caatccatct
     1801 caaagatata gtcgtaatta cgttccagtc cataacgaaa tccggtgatg tatgctgttc
     1861 ccaatcccag ctttcctttt cgttctatca gatatagatt ttcagaaaat tccttttgaa
     1921 gttcacggac aatggctgct gtcccatccg gactttcgtc atcgacaacg agaatgtgaa
     1981 atggtttcgg taaggaaaaa accttcctga ccatattctc aatattttcc ttctcattga
     2041 aggtgggaat gattactaga ctatcggaca aaggacgatg gatttattct tttatcagtt
     2101 ctatgatctt ctccaccaca tcttcagcat taggtttggt gaaatagtca ccatcagaac
     2161 cgaatggtgt tctgtgggct tttgccgtaa tgcacaacgg cttcgcgtct aaagaaaaat
     2221 atccatcctg actttccagt atttcttgta gcatataagc cgttgcacct cccggtacat
     2281 cttcatccag gattacaagt ctgtttgtct tagcgaggga tgcccctatg atatgattaa
     2341 tgtcaaaagg taaaagtgtc tgcacatcaa tcaattcgac agatatatcc atggtgtcca
     2401 atagcttgat ggcctcctga gcgaccctga cgcaactacc gtatgtgacc agtgttacat
     2461 cttctccttc gtgcaatatc tcagggattc ccagaggaac ggagaaggtg tcaaggttga
     2521 ccggacattt ctccttcaaa cgatatccgt tgagacattc tattaccagc gccggatcca
     2581 tgccatccat tagggtattg tacatgcctg ctgcctgggt catgttgcgt ggaacacaga
     2641 tgtgcatgcc ccggag
//
LOCUS       DAOQMC010000297         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_24180_length_2586_cov_4.999605, whole genome shotgun sequence.
ACCESSION   DAOQMC010000297 DAOQMC010000000
VERSION     DAOQMC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24180_length_2586_cov_4.999605"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..915
                     /locus_tag="PKV31_09585"
     CDS             424..915
                     /locus_tag="PKV31_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93151.1"
                     /translation="MNISKCVILTLGIMSLLSCKNDSHTTEHGSNESSHAAHVSTIDS
                     TQPPMFNEIMAVHDEIMPWMNNIETLKQDIRMKMDSIDAFGGSDANRKQFRHAMAELN
                     RADNAMTDWMSSFKENYDTIQIEKNKMVFLESERAKIEGVKNLMTESIKVAQLTLKNT
                     PIK"
     gene            934..1533
                     /locus_tag="PKV31_09590"
     CDS             934..1533
                     /locus_tag="PKV31_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB93152.1"
                     /translation="MTFRFFIFSLLLLVLNGCNTKPEPLPFLGIKVHEDDKPQIPEYH
                     FIDQLNRPVTPETFKGKIYMTDFFFTSCPTICPIVTGQMLRIYKEFEKDDRVAFLSHS
                     IDTRHDSVPKLKAYADKLHINDDKWRFITGKKEDIFNISKNYLSMVLEDPNTPGGFDH
                     SGYIILVDKDRHVRGFCNGTDPKDVDKFMGKVKQLLDTE"
     gene            1530..1913
                     /locus_tag="PKV31_09595"
     CDS             1530..1913
                     /locus_tag="PKV31_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB93153.1"
                     /translation="MTRWLIVLLSAVFVLYSGCTTYSQGKRIYESKCANCHGIKGEGL
                     AALYPALDKSIIITHHKSRIPCIIELGRSSRDSLGNLTSEMPPVKGLTVAEITNLINY
                     LLTLNPNGQETVTIDSVKVWSEACN"
     gene            2068..>2586
                     /locus_tag="PKV31_09600"
     CDS             2068..>2586
                     /locus_tag="PKV31_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93154.1"
                     /translation="MKYDAVGKIFVENCEATKGTIVFIFDNQQVERRFEVKFTGDATP
                     GTDFQVDGLSDTLVVGSGIQTIELPFTVNVDNVAEGREDLTITLVSIPEGQEIASYST
                     SIYDEFSVSINDGVQVESCKGQKLLLTASEAGTFLWTPSENFTDATKQTVEYVSNESS
                     TVHLTAGLGSCTA"
BASE COUNT          847 a          501 c          493 g          745 t
ORIGIN      
        1 taggctgaat gcgatacaag catttgattt tcataagctt cggattacct tatcagacac
       61 tttcaattta tattcaccgc tttccacctt caatagctta tacaataaaa caaaagcctg
      121 ttggagcaac accttgcttc actttctgat aaattcctga ttggcaggat aaaatatact
      181 attccaacaa gaagactttc gaagaaatgt cagacttata tgatattgag tatgtaaagt
      241 aaaactatac aagagaaaga taaaattaaa aaaattaaag cgatcaccgc caaagcttaa
      301 agcctacccg attgattatg tggcttttac aacatttatc caaactccga caaattaaaa
      361 tttatcagat atgactttta gtttgttgaa aattacttac ttttaccgga aatattttac
      421 cttatgaaca tatctaagtg tgttattcta accctgggga taatgtcttt actgagttgt
      481 aaaaatgact cccataccac agaacatggc tcgaatgaat catctcatgc cgctcatgtt
      541 tctacaattg acagcaccca accccctatg ttcaacgaaa tcatggcagt acatgatgag
      601 attatgccgt ggatgaacaa tatagaaaca ctaaagcagg atatccggat gaaaatggat
      661 tccatagatg cctttggagg tagcgatgcc aacagaaagc aattcagaca tgctatggca
      721 gagctcaata gagctgacaa cgcaatgact gattggatgt caagctttaa ggaaaactat
      781 gataccattc agatagaaaa aaacaaaatg gtattccttg aatcggaaag agctaaaatt
      841 gagggtgtta aaaatcttat gacagaaagt atcaaggtgg ctcaattgac cttgaaaaat
      901 acacccataa aataaatacg ctctcaaggc attatgacat tcagattttt cattttctct
      961 ttattgcttc tggtgcttaa tgggtgcaat accaagcccg aaccattacc cttcctcggt
     1021 ataaaagtac atgaggatga taagcctcaa attccggaat atcattttat cgatcagctt
     1081 aaccgcccgg ttacgcctga gacttttaaa ggcaaaatat atatgactga cttctttttc
     1141 acctcctgtc ctacaatttg cccgattgta acaggtcaaa tgctccgcat atataaggag
     1201 tttgaaaaag atgatcgggt ggccttcctt tctcattcca tagatacccg acatgactcg
     1261 gtacctaaac tgaaggccta tgcggataag ctgcacatca atgatgacaa atggagattc
     1321 attaccggca agaaagaaga catcttcaac atctcaaaga attacctgag tatggtgctg
     1381 gaagatccaa atactccggg agggtttgat cactccggat atattatcct tgtcgataaa
     1441 gacaggcatg tacgtggctt ttgcaatgga accgacccaa aggatgttga taaatttatg
     1501 ggcaaggtga agcaattact tgatactgaa tgacccggtg gctgattgta cttttgtcag
     1561 ctgtttttgt tctttattca ggctgcacca cgtactccca aggcaagcga atctatgagt
     1621 caaaatgcgc caactgtcac ggaatcaaag gggaaggtct agcagcactc tatccggcac
     1681 ttgataaatc catcatcatt actcatcata aatcacggat tccctgtatt atcgaactcg
     1741 gcaggagttc cagagacagt ctgggaaacc taacttcaga aatgcctcct gtcaaaggac
     1801 tgactgtagc tgaaatcact aatttgatca attacctgct aacattgaac cccaatggac
     1861 aggaaacagt aaccattgat tcagtcaagg tatggagtga ggcttgcaat taaatatttt
     1921 ttcttatcgc aagaaataat tatgtttaca tctgtttttt aaatgaatta tatgttctta
     1981 agattatctt taataggcct aatttcaatc ttttccctgt ttttaacaaa tcaaatactt
     2041 cttgctcaga attcaattgg aatagatatg aaatatgatg ccgtagggaa aatatttgtt
     2101 gaaaactgtg aagctactaa gggtacaata gtttttatat ttgataatca acaggttgaa
     2161 aggcgttttg aagtaaaatt tacaggagat gcgacacccg gcactgattt tcaggtcgat
     2221 ggacttagtg atactcttgt agtgggctcc ggaattcaaa ctattgaatt gccatttaca
     2281 gtgaatgtag ataatgtggc ggaaggaagg gaagacctga caatcaccct ggtttcaatt
     2341 cctgaaggtc aggaaattgc aagctacagt acaagtatat atgatgaatt tagtgttagt
     2401 atcaatgacg gtgtgcaggt agaaagctgt aaaggtcaaa agttactcct gacagcctcc
     2461 gaagcaggaa catttttatg gacaccttcc gaaaacttta ctgatgcaac caaacaaact
     2521 gtcgaatatg tcagcaatga atcatccaca gttcatctga ctgcaggcct gggttcttgc
     2581 acagct
//
LOCUS       DAOQMC010000298         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_24198_length_2585_cov_4.725692, whole genome shotgun sequence.
ACCESSION   DAOQMC010000298 DAOQMC010000000
VERSION     DAOQMC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24198_length_2585_cov_4.725692"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKV31_09605"
     CDS             complement(<1..242)
                     /locus_tag="PKV31_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB93155.1"
                     /translation="MANGLLKGKKGIIFGALDEKSLAWKVAERAYEEGASLVLTNAPV
                     ALRFGQINALAEKTGAPVIPADATSSEELAKLIDDS"
     gene            complement(272..1930)
                     /gene="recN"
                     /locus_tag="PKV31_09610"
     CDS             complement(272..1930)
                     /gene="recN"
                     /locus_tag="PKV31_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNB93156.1"
                     /translation="MLQRIRVRNYAIIDEVSIEFDPHFNIITGETGAGKSILLGALGL
                     ILGNRADSKTFFNTEEKCIVEAEFSIGSYSLHPWFEANDLDYEDLTLIRREIQPAGKN
                     RTFINDTPVTLQQLQEFTQQLIDIHQQFSLYDIQKPAYQLMLFDAYSGNKVLMGQYSE
                     LYRKYIALGRTLKELQEERQKSLREADYLAFQLEELNSVDFVNMDQDALEEELNMIDH
                     AGTIKSTAGAAYVALYDDDNNIISAIEDILNSFGPITAVSPRIKEVVDRLQSSRIELR
                     ELAEDINRLAESTEIDEERAAEIRESLDQLYKLQSKHQVGDIAGLVALKEEIDGKLNN
                     YSKVDDDIVRIEQEMSHHEEVLNRMAESLSESRKSHIPDFIDTLKGLLSDLGMPHATF
                     VIEMEPSTALTPSGKDAIRFLFAANKGGQPAAIKDVASGGEISRLTLSIKSMVAAAIP
                     LPSLIFDEIDTGLGGEIALKMAEIMREMAREHQLIAITHSPQLASKADKHFFVYKEIE
                     DNNTFTRIKELSLDERIENIAIMLSTKPPTKAAVENARELLERK"
     gene            complement(2151..>2585)
                     /locus_tag="PKV31_09615"
     CDS             complement(2151..>2585)
                     /locus_tag="PKV31_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93157.1"
                     /translation="DFLIEKLYKMKHAFKAFLLLMIFFIVGSVNAQNRSGDDGQASMR
                     AAKIKSAKKKDQSTQQKSNPAHASLFDNLDTNKDGKVSREEANKSNLVIIKEKFDNLD
                     TNKDGFLSKAEVQNARYNVNKGQQRTTSKVKSSTTNTAAKKQ"
BASE COUNT          640 a          612 c          519 g          814 t
ORIGIN      
        1 atactgtcat cgatcaactt agccagctct tcactggatg tggcatcggc agggataacg
       61 ggagctccgg tcttctctgc cagggcattg atttgtccga atctcaatgc aaccggcgca
      121 ttcgtcaata cgagggaagc gccctcttca taagcccttt ctgcaacttt ccaggcgagt
      181 gatttttcgt ccagcgctcc gaagatgatg cctttcttac ctttcaacag tccatttgcc
      241 ataattcttt ctcttttaat attttgatta atcactttct ttccagaagc tctcttgcat
      301 tctcgaccgc tgccttggta ggtggtttgg tcgaaagcat gatagctata ttctcaattc
      361 tctcatccaa agataattcc ttaatgcgag taaatgtatt attgtcttct atttctttat
      421 aaacgaaaaa atgtttatct gcttttgaag ccaactgcgg tgaatgtgtg atggctatca
      481 gttgatgctc acgcgccatt tcgcgcatga tttcagccat tttcagtgca atttcaccac
      541 cgagtccggt atcgatctca tcaaatatca gggaaggcaa cggaattgct gcggccacca
      601 tagacttgat cgaaagggta agtctggata tctcccctcc tgaggcaaca tccttgatgg
      661 ccgcaggctg accaccctta ttggctgcaa aaagaaatcg aatggcatct tttcctgatg
      721 gtgtaagagc cgtggatggt tccatttcaa ttacaaatgt ggcatgaggc atacccagat
      781 ccgacaacag tcccttaagt gtgtcgatga agtcgggaat atggcttttc cttgattctg
      841 aaagactttc tgccatcctg ttcaatactt cttcatgatg actcatctct tgttctatcc
      901 gtacaatatc atcatccacc ttggagtaat tattcagctt gccatctatt tcctccttca
      961 aggcaacaag accagctata tcccctacct ggtgcttgct ttgcaattta tacaactgat
     1021 ccagcgactc cctgatctct gcagctctct cttcatcaat ctccgtactt tcagccagtc
     1081 ggttgatatc ctctgccaac tctctcaact ctatcctgga ggactgaagt ctgtcaacca
     1141 cctccttgat tctgggagag actgccgtga ttggaccaaa actgttgaga atgtcctcta
     1201 tcgctgaaat gatattgttg tcgtcatcat aaagtgcaac ataggctgcg ccggcggttg
     1261 atttgatggt acccgcatgg tcgatcatgt tgagttcttc ttccagtgcg tcttgatcca
     1321 tattgacaaa atccacacta ttcaattcct ccagctgaaa agcaagataa tctgcctctc
     1381 gtaacgattt ttgccgttct tcctgcaatt ctttcaatgt acgtccgagg gcaatatatt
     1441 tccggtaaag ttcagaatat tgccccatca acaccttgtt gccgctgtat gcatcaaaga
     1501 gcatcaactg ataagccggt ttctgtatgt catagaggct gaattgttgg tgtatatcga
     1561 tcagttgctg ggtaaattcc tgcaattgtt gcaaggtcac aggtgtatcg ttgataaagg
     1621 tgcggttttt acctgccggt tgaatctctc tgcggatcaa cgtcagatcc tcataatcca
     1681 gatcattggc ctcaaaccag ggatggaggc tgtagcttcc tatgctgaat tctgcctcga
     1741 caatgcattt ctcttcagta ttgaagaagg tcttgctgtc ggcacgattg cccaaaatca
     1801 atcccagcgc acccagtaag atggactttc cggcacctgt ctcaccggta ataatattga
     1861 aatgcgggtc gaattcaata ctgacctcat cgatgatggc ataatttctg acccggattc
     1921 gttgcaacat aataaatagt ctgattgacg ggcgaagtta aggaaaagag acgtcttcgt
     1981 atatcgattc cggcaatttt caattttgaa ttgaacacca acagacagat tcaacactcc
     2041 taaaaagtga gcaagaaaaa aaggtgccgt cgcattcact acaaaacggc accttttccg
     2101 gtgcgacaga tccttattgg ttacagacaa tcaccaccaa ccggttatca tcattgcttt
     2161 ttggctgcgg tattggtagt gctgcttttt acttttgatg tcgtacgctg ctgtccctta
     2221 ttgacattgt atctggcatt ttggacctcg gcttttgaaa gaaagccatc cttattggta
     2281 tccaggttat caaatttctc ctttatgatt accagattac ttttattggc ttcttcccgg
     2341 ctcactttgc catctttatt cgtatccagg ttatcgaaca aagacgcatg tgcgggattg
     2401 gatttttgtt gggttgattg atcctttttt ttggcgctct tgatttttgc cgctcgcatg
     2461 gaagcctgtc catcatcacc acttctgttt tgtgcattga ctgaaccgac aataaagaat
     2521 atcatcaaaa gtaaaaaagc tttgaacgca tgtttcattt tatataattt ttcgataaga
     2581 aaatc
//
LOCUS       DAOQMC010000299         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_24302_length_2578_cov_5.265557, whole genome shotgun sequence.
ACCESSION   DAOQMC010000299 DAOQMC010000000
VERSION     DAOQMC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24302_length_2578_cov_5.265557"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKV31_09620"
     CDS             complement(<1..182)
                     /locus_tag="PKV31_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93158.1"
                     /translation="MGNPPALPEDWKSLTFQGVHQETSSREPPSTRLWRIPMDESESL
                     SRRSRNQTGQGFGHSL"
     gene            complement(189..2411)
                     /locus_tag="PKV31_09625"
     CDS             complement(189..2411)
                     /locus_tag="PKV31_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93159.1"
                     /translation="MAIFEKERIKEYDDYLSVLTRHGYAKYIRPFVDILIIQVQPQAN
                     SKARDEDKKDWFAIKPANDDIFATLSRLAESLTLPCCQLSDTTFGIGFQAKDASQRQV
                     IMSGYGLIEDKVFDSSMDCNNLSLEELASGTGTFEAFILEDDEVFACNDYFGMGRLFI
                     YSWNGGALVTNRAHLAAAVIRLARLPLKLNEDYMRTLVLEQHVFSISGFNEESPIKNL
                     TKLPIRHLARISEGGAYCKHHIFPKKVANEECYQDLLLRAKSSLVTSMRKFIEYFKNN
                     GYEIVCDVTGGKDSRILLSLMLDIGEKFNVRTIDQEDDLLYSNMICSRYRLKYIDSEP
                     DFYAKINAAYYFDIMNSYTMGNNNTQSLPGSTQGFGQNGRVIFTGGGGEIYREQWSTH
                     FHCLSGESVADNVVRLMDKNSAMIDKYLSSDEIEKLRFYLIDSFSKFDEISPRYEHYL
                     HNQLRSHFGITGNLAFVNSFLHHQILSKDLQEAYLALSVEDRENGRCIYDFIFNIDQS
                     LADLPYTGGFPYADSRRQNSIPDKPDPQDEMVNAKLEEWNTCRKRKLAKASLPVKAVG
                     EAISDPRRYLHILFCKATCRLLASFPSLKGILEQVANVVNLYLYSVPSNRLISRCYHT
                     IGCENFLFPLNSEKYEICDDDVKKYLTPIRNFTFNVRKSSIEVSMVLFDDCNRDDYVY
                     KFHAYSNHSKKAISSVVTAEFPNCLLSDVEAAPDMTIVGTCIHKKNKRRFWGVSEKIN
                     "
BASE COUNT          723 a          573 c          495 g          787 t
ORIGIN      
        1 ctaaggctat gaccaaaacc ttgacctgtt tggttccggc ttcgccggct taggctttcg
       61 gactcgtcca tcgggattct ccatagtcgt gtgcttggcg gctcacggct tgaagtttcc
      121 tgatggactc cctgaaatgt caaacttttc cagtcctctg gcagagccgg gggatttccc
      181 atttttggtt aatttatttt ttcactcact ccccagaatc gtcttttgtt ctttttatga
      241 atgcatgttc ccacaatagt catatctggt gctgcttcta catcagacaa aaggcaatta
      301 gggaattccg ctgtaacaac acttgaaata gcttttttac tatgattact ataggcatgg
      361 aatttataga cataatcatc tctattacaa tcatcaaaga gcaccataga aacctcaatt
      421 gagcttttcc gaacattgaa agtgaagttt ctgattgggg taagatattt tttgacgtca
      481 tcatcacaga tttcgtactt ctctgagttg agcgggaaaa ggaaattctc gcaaccaata
      541 gtatgataac atcgtgaaat aagtctattg ctaggcactg aataaaggta aagattaact
      601 acattcgcaa cctgttcgag tattcctttc agtgagggaa aggatgccaa aaggcgacag
      661 gtagccttgc agaacagtat atgtaaatat cgtctagggt cggatattgc ctctccgaca
      721 gctttaactg gtaatgaggc tttcgccagc ttgcgcttgc gacaagtatt ccattcttcc
      781 agtttcgcat tcaccatctc atcctgtgga tccggtttat ctggaattga gttctgccgt
      841 ctactatcag catagggaaa accccctgta tacggtaaat cagccagaga ttggtctata
      901 ttgaagataa agtcataaat acatcggcca ttttctcgat cttccacact aagggccaga
      961 taggcctctt ggagatcttt agagagaatc tggtgatgaa gaaaactatt gacaaacgcc
     1021 aagtttcctg ttatgccgaa atgactacga agctgattgt ggaggtaatg ctcatatcta
     1081 ggggatattt cgtcaaactt gctgaaactg tcaattaagt aaaagcgtag tttttctatt
     1141 tcatccgacg agagatattt atcaatcatg gcgctatttt tatccatcaa tctcacgaca
     1201 ttatcagcta ctgactcacc tgacaaacaa tggaaatgag tactccattg ttcacggtaa
     1261 atttcccctc ctcctcctgt aaatataact cttccatttt gtccaaatcc ctgcgtgctc
     1321 cctggaaggg attgtgtatt gttattaccc atcgtgtagc tgttcataat atcaaaataa
     1381 taggcggcat taatcttggc atagaagtcg ggctcactgt cgatatattt taacctatag
     1441 cgcgaacata tcatattcga ataaagtaga tcgtcttctt ggtcgatagt gcgtacatta
     1501 aacttttctc caatatctag catgagcgat agcaatatac gagaatcttt tccaccagtt
     1561 acatcgcata cgatttcgta gccattgttc ttaaagtact caataaattt tctcattgaa
     1621 gtaacaagac ttgacttggc gcgaagtaac aaatcctgat aacattcttc attggctact
     1681 ttcttcggga agatatgatg cttgcagtaa gctcccccct ccgatattcg cgctaaatgc
     1741 cgtattggta attttgttag gttctttatg ggggattctt cattaaaacc agaaatggaa
     1801 aatacatgct gctcaagaac aagagttctc atgtaatcct catttagctt taaaggcaat
     1861 cttgccagcc taatcacagc agcagctagg tgggcacggt tcgtaacaag agcgcccccg
     1921 ttccaagaat atatgaagag cctgcccata ccaaaataat cattgcaagc gaagacctca
     1981 tcatcctcaa gaataaacgc ttcaaatgtg ccagtacctg atgccagttc ctctaagctc
     2041 aggttattgc aatccatgga actatcgaat actttgtctt cgattagacc ataaccactc
     2101 attatcacct gcctttgcga cgcgtccttt gcctgaaaac caatcccaaa agttgtgtcc
     2161 gacaactgac aacaaggaag ggtcaatgac tcagctaacc gcgataatgt ggcaaagata
     2221 tcatcattcg ctggcttaat tgcaaaccaa tcttttttgt cttcatccct tgctttggaa
     2281 ttcgcctgtg gctgcacctg aatgattaaa atgtcgacaa aaggccttat atatttagca
     2341 taaccatgcc tcgtcagcac agaaagataa tcatcgtatt cttttatacg ttctttttca
     2401 aagatagcca tggctattca ttcctctgag ctaaaaacgc ttggcgacgt gcgaagcgtt
     2461 ggtccataaa tgtggcgctg gtatagtata ttgacatact tcctgtaatg tatgcgttta
     2521 gcccatatcc ttacacacaa ctcattttgc atgtttgcca aactttttgc gggtgcgt
//
LOCUS       DAOQMC010000300         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_24782_length_2552_cov_5.155386, whole genome shotgun sequence.
ACCESSION   DAOQMC010000300 DAOQMC010000000
VERSION     DAOQMC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24782_length_2552_cov_5.155386"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKV31_09630"
     CDS             complement(<1..644)
                     /locus_tag="PKV31_09630"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNB93160.1"
                     /translation="MIKRSFIIISLAFMSIMTGSTQTIMHRIAIGSNLDQEGSQRIID
                     LIARRKPDAFISLGNIITGGSANQVEMREKYGTLSEWQGLKKLKESTKFFATWDDHEY
                     GESEGGKHFSDKGAYKQLFLDFAGDPDNSLRKSHEGVYNAYGYGNAGSRVSIILLDTR
                     TFRDNLLPYQGDRRDDPRFFHPLQYSPYKSADSTLLGEDQWIWLERQLMEPALV"
     gene            1174..>2552
                     /locus_tag="PKV31_09635"
     CDS             1174..>2552
                     /locus_tag="PKV31_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HNB93161.1"
                     /translation="MAKMYYILIFLSLGTIMLGQSKAEDKGKPRDEKPGKYDRSLDFD
                     YKVEKNIQSVINGKNVPYKVTAATLPVWDEEGKAMAGVFYTYYERTDISDKSTRPLVF
                     SFNGGPGTASVWMMLGYTGPRLLHIDDEGYPLQPYGLKDNPNSILDVADLVYVDPVNT
                     GFSRPTNKDIPRDKFFGVNADVRYLADWINSFVNKYQRWASPKFLIGESYGTTRVSGL
                     ALELQNSHWMYLNGVILVSPTDLGIKRDGPVGRALMLPYYTATAWYHKALAPALQNKD
                     LLDILPEVERYTIEEYLPALALGSSIPPEKYNAMVKMVSYYSGISEKSVRENNGIIYT
                     NFFWKELLRDRGYTVGRLDSRYLGIDSRDAGMGPEYNAELTTWLHAFTPAINMYMRDE
                     LGYRTDLDYNMFGPVHPWNSDNNNTGNNLRAAMAQNPFLHVLVQSGYYDGACDYFNAK
                     YNLWQMDPNG"
BASE COUNT          685 a          513 c          592 g          762 t
ORIGIN      
        1 tgaaccaggg caggttccat caattgtctt tccagccaaa tccattgatc ttcacccaga
       61 agtgtgctgt cagcagattt atagggagaa tattgcaaag gatggaagaa tcttgggtcg
      121 tctcttctat caccctgata cggcaaaaga ttgtcgcgaa atgtccgggt atcaagtaaa
      181 atgatgctga cgcgagagcc ggcattcccg tagccgtaag cattatagac accttcatgg
      241 cttttgcgca gggagttgtc cggatcacct gcgaagtcca ggaaaagttg cttgtatgct
      301 cctttgtcag aaaagtgttt gccaccttct gattcgccat attcatggtc atcccatgta
      361 gcaaaaaatt ttgtcgattc cttcaatttt tttaggcctt gccattctga caaagtaccg
      421 tatttttcgc gcatttcgac ctgattcgca cttccacctg taataatatt tcccagagaa
      481 atgaatgcat cgggtttcct gcgagctatc agatcgataa ttctttgaga accctcctga
      541 tccagattgc ttcctatggc tatcctgtgc atgattgttt gggtactccc ggtcataatt
      601 gacataaaag ccagggagat aataataaaa cttctcttta tcataggtca aaaatataaa
      661 tttatcctga cccgactatc cggttgaatt tcttgtcatt gcattaatat tatattttat
      721 atttaacctt aatatatata ataacttata ttgggttcgt tgtaaaatta tcactctgtt
      781 aaaatttgtc aacctaatca tgttgttttc tgaagaactt gcccggatat tcatgggtcc
      841 atttttagtg ataagtagga atagtaccaa ccgatgaggt ttattattgt ctattgagct
      901 aaattcaact tgttgttcgc agcacaaaat ttttttcggg ataggagaag ggtgcaattc
      961 gttttggacg cttcggtttg atgcccagga tcctgaaatc agctcaaagg tacgggtagg
     1021 ttcattgtgg cgactgcatt ctatatacat tgtcaatcgg gaaatgaagt gattttatcc
     1081 gatgtttcac taattatgga gaataaatgc cttttcctgc cttattcttg agtactcttg
     1141 cttatatttg atactttcat taaaattctc gatatggcaa agatgtatta tatattgatt
     1201 ttcctttctc tgggcactat tatgctcgga caatctaaag ctgaagacaa aggcaagcca
     1261 agggatgaaa agcccggaaa gtatgatcgc agccttgatt ttgactataa ggtcgagaag
     1321 aatattcagt ccgtgatcaa tggcaagaat gttccgtata aggtaactgc tgccaccctg
     1381 ccggtatggg atgaggaggg taaggcgatg gcgggagtat tttatactta ttatgagcgt
     1441 acggacatat ccgacaagtc cacacgtcca ttggtgtttt cattcaatgg aggccccgga
     1501 acagcttctg tatggatgat gctgggatat acaggccccc gattgctgca catcgatgat
     1561 gaagggtatc cgctgcagcc atatggtcta aaggacaatc ccaattccat cctcgatgtg
     1621 gcagatttgg tttatgtcga tccggtcaat accggatttt cgcgaccgac taacaaggat
     1681 ataccgcgtg acaagttttt tggggttaat gccgacgtaa ggtatcttgc tgactggatc
     1741 aactcttttg tcaacaaata ccagcggtgg gcctctccca agtttctgat tggagagagt
     1801 tatggcacca cacgtgtttc cgggctggca cttgagttac aaaacagcca ttggatgtat
     1861 ctcaatggtg tgattctggt atctccgact gatttgggga taaagcgaga tgggcctgta
     1921 ggaagagcgc tcatgctgcc atactatacg gcgactgcat ggtatcataa agccctggca
     1981 ccggccttgc agaataagga tttgctggac atcctgcctg aggtagagcg atatacgata
     2041 gaggaatatc tgcctgcttt ggcattggga agttcgatac cgccggagaa gtacaatgca
     2101 atggtaaaga tggtaagtta ttattcaggc attagtgaga aatcagtccg tgaaaacaat
     2161 ggtatcatat acaccaattt cttttggaag gagttattac gcgatcgcgg atatactgtt
     2221 ggtcgtcttg actcgaggta tctgggtatc gactcacgtg atgcaggcat gggacccgag
     2281 tataatgccg agttaacgac ctggctacat gcttttacac cggctattaa catgtatatg
     2341 cgggatgagt tgggctatag gacggatctg gattacaata tgttcggtcc ggtccatcca
     2401 tggaatagtg ataacaacaa taccggcaac aacctgagag ctgcgatggc acaaaaccct
     2461 ttcctgcacg tattggtgca gtcgggatac tatgatggag cttgtgatta tttcaatgcc
     2521 aaatacaatc tgtggcagat ggatcccaat gg
//
LOCUS       DAOQMC010000301         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_25004_length_2538_cov_5.387435, whole genome shotgun sequence.
ACCESSION   DAOQMC010000301 DAOQMC010000000
VERSION     DAOQMC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25004_length_2538_cov_5.387435"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKV31_09640"
     CDS             <1..468
                     /locus_tag="PKV31_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB93162.1"
                     /translation="GITKDGLLLRMSEEMWDAVIDTNLKSVFNLTKHAIRPMLRTGGS
                     IINMSSVVGVFGNAGQANYSASKAGIIGFTKSVAKELGSKNIRCNAIAPGYIATEMTQ
                     VLDESVKEGFMANVPLKRLGEAEEIANVTMFLASDLSSYITGQVLNVCGGMAI"
     gene            485..976
                     /locus_tag="PKV31_09645"
     CDS             485..976
                     /locus_tag="PKV31_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93163.1"
                     /translation="MSSLKELEKFNPDSKVWVFTANRVLSDTEITQINSRLEEFAQSW
                     VSHGDPLSARAFVLHRYFVILAVDNKMYLASGCSIDTAMRFIQTLQIDFDVDLLDRQS
                     FVYLKDHKPVLIRRKDMKSAYRDGIILDDTLFFDPLVTSWQEMREGFLKPMRDFWMKN
                     MIR"
     gene            1315..>2538
                     /locus_tag="PKV31_09650"
     CDS             1315..>2538
                     /locus_tag="PKV31_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNB93164.1"
                     /translation="MKLTVFKMIRFVYISLILCVTFGYVYGQYQMPVYYATSYRPGQG
                     GEEVGNAIDGNDATLYHSRYDLYAVPDTLTFYFNPNVPEINKIEYSPRKERYNGIWTK
                     VDIMYSKRDKPDQFIRLNSDPIIWDLNNDVKSVNLDSALEHVFAIRIAVHEAYDDYSS
                     CAEMRFYGTKPALPDGSIDCSLDASTLQGNKDAKIKIDEAGSSASSYQYYENIEKSFD
                     GNFSTLYHSNYNAGQDAFPIDLVYHFSNKPVIDYFIYYPRSDGGYNGFFGATSIYYNT
                     EESGPGAEYTHLLDYDFKMEGLPVRIAFPTSLKVNNVKIVVKNGASGFASCAEMEFYE
                     KNGGDPNVFEYEQIFTDRLYTDLLPLVTQEMIDTISVPFYKQLASCLYAGTYNRSLRI
                     RDFKAFESTGQLSDKL"
BASE COUNT          736 a          490 c          549 g          763 t
ORIGIN      
        1 ggaataacca aagatggcct tttactcagg atgtcagaag agatgtggga tgctgtcatt
       61 gatactaacc ttaaatccgt gttcaacctg accaaacacg ccatccggcc tatgttgcgt
      121 acaggtggca gtatcatcaa tatgagctct gtagtaggtg tgtttggcaa tgccggacag
      181 gccaattact ccgcctcaaa agccggtatc atcggtttta caaagtcagt tgctaaggaa
      241 ttgggcagta agaatatcag atgtaatgcc attgcacccg gttatatagc taccgaaatg
      301 acgcaggtgc tggacgaaag cgtaaaagaa ggttttatgg ccaatgttcc gttgaaacgc
      361 cttggggaag ctgaagaaat cgcaaatgtg actatgttcc tggcttctga cctttcatcc
      421 tacatcaccg gccaggtgct caatgtatgc ggcggaatgg ccatctaatc cataacgaat
      481 caatatgtct tcattgaagg aacttgaaaa atttaatccg gacagcaagg tttgggtttt
      541 taccgccaat cgcgtattgt ccgatacgga aattacccaa atcaattccc gactggaaga
      601 atttgcacaa tcctgggtga gtcatggtga tccattgagt gcacgggcct tcgttttaca
      661 ccgatatttc gtcattctcg ccgttgataa taaaatgtat ttggcaagcg gttgttctat
      721 tgatactgct atgcgattta ttcaaacact tcagatagac tttgatgtcg atcttcttga
      781 cagacaatct tttgtctatc tgaaggatca caaaccggtt ttgatccgta gaaaagatat
      841 gaagtcggcc taccgggatg gaatcattct tgacgacacc ttgtttttcg accctttggt
      901 gaccagttgg caggaaatga gagaaggctt tctgaaacca atgcgtgatt tctggatgaa
      961 gaatatgatc cggtaattgc attcagattc ctgatccgga ctataggcaa atcaaatttt
     1021 ttataacaaa ctaattttgt ttgaggggca aaagctgtat aatttgataa gcaattcatt
     1081 ccggaatatt tttttgcaac cacgatactg tcctcggtat taatctgatt gccctctcct
     1141 tcattgaaag acacagatcg aacaataatg caataaagtg tcggtgatag tgaccaactt
     1201 acctttggtg atggatgaag ctgtttgaga aaatttcaca atattattag gagtgcatcc
     1261 aaaaaatccg attctgcaca aaataatata atttcttctg ccaattgaat acctttgaag
     1321 cttactgttt tcaagatgat cagatttgtt tatatttcat taattctttg tgttacattt
     1381 ggttatgtgt atggtcagta ccaaatgccg gtatattacg caacttccta caggcccggt
     1441 caaggtggcg aagaggtagg caatgccatt gatggtaatg atgccaccct ctatcattcg
     1501 aggtatgacc tctatgcggt gccggacaca ttgacctttt actttaatcc taatgttcct
     1561 gagatcaata aaatagaata cagcccgaga aaagagagat ataatggtat ttggactaag
     1621 gtagacatca tgtattcaaa aagagataag ccggatcaat tcatcagatt gaattccgat
     1681 ccgattatct gggatttgaa taatgatgtc aagtccgtga atcttgactc cgccctggag
     1741 cacgtctttg cgatcaggat agctgttcat gaagcctatg atgattattc cagttgtgcg
     1801 gaaatgagat tctacgggac taagcctgca ttgcctgacg ggagcattga ttgcagtctg
     1861 gatgcttcca ctctccaggg taataaggat gccaaaataa aaatagacga agcgggatct
     1921 tcagcatcat cataccaata ttatgagaat attgaaaaga gttttgatgg taatttttcg
     1981 accttatacc attcaaatta taatgcaggt caggatgctt ttcctattga tctcgtttat
     2041 cattttagca ataagcccgt gatagattat ttcatttatt atccaagaag tgatggaggc
     2101 tacaatggtt tttttggtgc tacttccatt tattacaata cggaagagtc cgggcctggt
     2161 gctgaataca cgcatttgct ggattatgat ttcaaaatgg aaggcctgcc ggtcaggatc
     2221 gcttttccga cttccttgaa agtcaataat gtcaagatag tcgttaaaaa tggggcaagt
     2281 ggctttgcca gttgtgctga gatggaattt tatgaaaaaa atggtggaga tcctaatgta
     2341 tttgagtatg aacaaatctt tacggacagg ttatataccg accttcttcc cttggtcact
     2401 caagaaatga tagataccat cagtgttcca ttctacaagc aactggcatc atgtctttat
     2461 gccggcacct acaatcggtc acttcggatc agagatttta aggcctttga atccaccgga
     2521 caattaagcg ataaattg
//
LOCUS       DAOQMC010000302         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_25619_length_2507_cov_4.174959, whole genome shotgun sequence.
ACCESSION   DAOQMC010000302 DAOQMC010000000
VERSION     DAOQMC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25619_length_2507_cov_4.174959"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKV31_09655"
     CDS             complement(<1..255)
                     /locus_tag="PKV31_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93165.1"
                     /translation="MKLKSLKRPLIVIGAMIIVAAFIFKGSIVPNVTSAAETKFIFVR
                     KGQSLKSLADSLEAGGLVKNGSAILKTAGIMGFKDSDLVPG"
     gene            complement(301..1116)
                     /gene="panB"
                     /locus_tag="PKV31_09660"
     CDS             complement(301..1116)
                     /gene="panB"
                     /locus_tag="PKV31_09660"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNB93166.1"
                     /translation="MSIQKEIKRVTTHIMQEMKNNGEKISMLTGYDYSMARIIDDAGV
                     DIILVGDSASNVMAGHETTLPITLDQMIYHASSVIRAVKRSFVVVDLPFGSYQGDSKK
                     ALNSAIRIMKESGAHGVKLEGGREVGESVKRILTAGIPVMGHLGLTPQSIYKFGTYSV
                     RAKEEEEAQRLVEDSQYLESLGCFSIVLEKIPASLAEKVATTLTIPVIGIGAGGGVDG
                     QVLVTHDLLGINKEFKPRFLRLYEDLYSRMFDAFSAYIRDVKSGEFPNKSEQY"
     gene            1220..2257
                     /locus_tag="PKV31_09665"
     CDS             1220..2257
                     /locus_tag="PKV31_09665"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNB93167.1"
                     /translation="MRQIVNYSLFCVFSISVLFFSSCNNELELIGDKVEIPVVYGTIS
                     TTGDFVFIRLERAFGDNKISPGILSKNPDSLYYKDAKVTISLPEENFSIDLEKTDAES
                     LGIKRDTGVFANTPNYIYVAPNDQFVFKPGAEYQLTIEVKGKTYTAKTTLVNTIEIIL
                     PTHTSQVAWIPRTPELQSQYPRVLYEPKGENRSADPAILDLHIRFNYSEKDSRKGNTY
                     EKKSFLIPVTGSIITDANTDFRYSPNRIFEYLRDHMEKDPAFDRFFTSFDFVITGGGR
                     EFLNFTEALAANAGITGTQDFPLYSNIEGGQGIFSSKNTMEFKGYNISSASLDSLAFS
                     RYTIGLNFRKI"
BASE COUNT          755 a          547 c          513 g          692 t
ORIGIN      
        1 acccggaacc aggtccgaat ccttaaagcc catgataccg gcagttttca aaatagcaga
       61 gccgtttttg accaagcctc ctgcttccaa ggagtctgca aggctcttca ggctttgacc
      121 ttttcgaaca aaaataaatt tcgtttccgc tgcagaagtg acatttggga caatgctgcc
      181 tttaaaaata aatgcagcta caattatcat agctcctatg acaatcaggg ggcgcttgag
      241 ggattttaat ttcattgagc agtagttatt ctgttaaaag gataattaaa ggcagttcaa
      301 ttagtattgt tcactcttat tcggaaattc accggatttt acatctctga tataagcaga
      361 gaaggcatcg aacattcggg aataaaggtc ttcatacagt ctgagaaatc gcggcttgaa
      421 ttctttattg atccccaaaa ggtcatgtgt caccagcact tgtccatcta cacctccacc
      481 tgcgccaata ccgataacag gaatggtcag ggtcgtggcg actttctcag ccaatgaagc
      541 gggaatcttc tccaacacga tagaaaaaca ccccagggac tcaaggtatt gggaatcctc
      601 aaccagacgt tgagcctctt cttcttcttt ggcacgtacg gaataggtac cgaatttgta
      661 gatactttgt ggggtcaatc caaggtggcc catgacagga attcctgccg tgaggatacg
      721 cttaaccgac tctcccactt cacgccctcc ttcaagcttt accccatggg ctccggattc
      781 cttcatgatt ctaatggcgc tgttgagtgc tttcttgcta tctccctggt aagaaccaaa
      841 aggaaggtca acaaccacaa agctgcgttt aactgccctg atgactgatg aagcatggta
      901 gatcatctgg tccagcgtga tggggagtgt tgtttcatga ccggccatga cattggaggc
      961 agaatcaccg accagaatga tgtcaacccc agcatcatct atgatcctgg ccatactgta
     1021 atcatagccc gtcaacatgc taatcttctc cccattgttc ttcatctcct gcatgatgtg
     1081 ggtagtaacc cgcttgattt ccttttgaat tgacataaaa acgaatgtta aaaaatgtgc
     1141 tgcaagttaa aaaaatgcct aatttcgcag gaattttatt tgaagattaa tcatccggct
     1201 aaattaattg aatacaaata tgcgtcaaat agtaaattat tccctctttt gtgtcttctc
     1261 tatttccgta ttatttttca gttcatgcaa caatgagttg gaattgatcg gtgacaaagt
     1321 cgaaatacct gtggtgtatg gaactatttc caccaccggc gattttgttt ttatcagact
     1381 ggagcgtgct tttggtgata ataaaatcag tcccggcatt ttatcaaaaa acccggatag
     1441 cctttattat aaagatgcca aagtaacaat cagcctgcct gaggagaatt tttcaatcga
     1501 tttagagaag actgatgctg aatccctggg cataaaaaga gacaccggag tttttgccaa
     1561 tacacccaac tatatatatg tcgctccaaa cgaccaattt gtgttcaaac cgggggctga
     1621 gtatcaacta acgattgagg tcaaaggcaa aacctacaca gctaaaacca cgcttgtcaa
     1681 taccatagag atcatcctcc cgactcacac cagtcaggtg gcatggatac ctcgaactcc
     1741 ggaacttcaa agccagtatc ccagagtact ttatgaacca aaaggagaaa acaggagtgc
     1801 agatccggcc atcctagact tgcatatcag gtttaattat tcagagaaag acagcaggaa
     1861 aggaaataca tatgaaaaaa agagtttctt gataccggtg accggatcca tcataacaga
     1921 tgccaatact gacttcaggt attcgcctaa cagaatcttt gaatacctta gagatcatat
     1981 ggaaaaagac ccggcatttg acaggttctt tacctcattt gacttcgtga ttacaggtgg
     2041 tggcagggaa tttctcaact ttacagaggc attggcagct aatgccggta tcaccggcac
     2101 ccaggatttt ccgctttatt cgaatataga aggcggacag gggatctttt cttccaagaa
     2161 caccatggaa ttcaaaggct ataatataag ctcagcttct cttgattcac tagcattcag
     2221 cagatataca atcggcctga atttcagaaa gatctaaaaa cttaagatga tctgaaggtc
     2281 ccttaattca atgccatgaa atttactttt atggagttaa ttctcattgc tgtttaatat
     2341 tgaggatctt ggcaaaaatt cagcttttgt ttcaaaaagc gcctttacat ctatcttagg
     2401 gtgacatagc cggaataaac aaatagaagc caaaggatat ggcgtaacgg ttgatatgag
     2461 cggggaaaaa tcgaagtagg taggaaagta atagctatga actccct
//
LOCUS       DAOQMC010000303         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_25692_length_2503_cov_4.216503, whole genome shotgun sequence.
ACCESSION   DAOQMC010000303 DAOQMC010000000
VERSION     DAOQMC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25692_length_2503_cov_4.216503"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..216)
                     /locus_tag="PKV31_09670"
     CDS             complement(4..216)
                     /locus_tag="PKV31_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93168.1"
                     /translation="MDTRLRCIVVEDEPHIRASLISKLSTFEELEIVGEAASISQAFK
                     LISEKRPDSAFIPDYALEKGKSKFWD"
     gene            complement(311..>2503)
                     /locus_tag="PKV31_09675"
     CDS             complement(311..>2503)
                     /locus_tag="PKV31_09675"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB93169.1"
                     /translation="KPNFVTKDAAGNFWIGTERAVQKILCYQYSIPLGTTGLLNSVWT
                     IQQSSDSNLWLGSFGEGLTIFDGYKFRRPPGNFNDLSKAQLANSTIRMHDGTMLFNVH
                     SSKFLTTGVLAVKDKKWKWLMDKKLGIYFDRDRHGRLMVGTFSDGLYIFRDDKKLTSD
                     NLYKVIDKKKGLKLDVVLAAVQDRYGRYWMGRASQGIAVYDPGKDTVINWLIRDKKKD
                     IGAISIAEDRRGNLWFGGLTGLYFLKNRPDIEAIQEMRKECIKVGEEIFEKNELNEIN
                     EIELVDDSTLLVGYPKGYFLIDLNKFYTNPQICPLRGAFKMKNENYTLGSIEQNSFLR
                     SKDGNYWLVTVDGIVSHNPFKYKPTNPPGDVFIDSMKVGNRLFTSFEDKIKLANEQRT
                     LSLYFSVPVDSLLYHNLSFQYRLNNDDWSPLQRESQILFQNLNSGDYNLQIRAVKDGN
                     YSPVKNISFAILPPLWLRWQVWLVSFIIFTLLGLYLYSRQRTIYRQKLSLAQKETEFE
                     LMNREKDTLRVSAIVNQLNPHFINNALQWLQVKMEDDVESISVIGRLAENINTVFRNS
                     RKNISFHGLANELQLASNYLYIQATRFGPGLKYRMPELSRDDFIMQVDVPIMIIQIHV
                     ENAVEHGIRNTDTGDGEVSVTVREDGEYIVIAIEDNGVGREKAALLGSKGTQNGTTML
                     RELEKIYNKNNRLKISQTYYDGIFTEETGEKYGTRVVIRIPKAYSYKI"
BASE COUNT          679 a          619 c          434 g          771 t
ORIGIN      
        1 gaatcaatcc caaaactttg acttcccttt ttctaatgcg taatctggga taaatgcaga
       61 atccggcctc ttctccgaaa tgagcttaaa agcctggctg atactggccg cctctcctac
      121 aatctccaac tcttcaaacg tcgaaagctt gcttataaga ctagccctga tatgcggctc
      181 atcttccacc acgatacatc ttagcctggt atccatccac ttttcatttt ttcagtgctc
      241 tatttgggtt gtctttcttc ataatcagaa aggttgttta tattaagggt caatttagta
      301 aaacattcgt ttatatctta tatgaatagg ctttcggtat ccggatgaca actctggttc
      361 catacttttc gcctgtctcc tccgtaaaga tgccgtcata atacgtctga cttatcttaa
      421 gcctgttatt tttattatat attttctcaa gttccctgag catcgtggta ccattctgcg
      481 tacccttcga acccagcaat gccgccttct cccgacctac gccattatct tcaatcgcga
      541 tgactatata ctctccatcc tccctgaccg taacactgac ctctccgtca cccgtatctg
      601 tatttcgaat accatgttca accgcattct ccacgtggat ctggataatc ataatcggaa
      661 catctacctg catgatgaaa tcatctctgc tcagctcggg catcctgtac ttcaatcccg
      721 gaccaaaccg cgtcgcctga atatacagat agtttgatgc cagttgaagt tcatttgcaa
      781 ggccgtgaaa actaatattc tttctgctgt tccggaaaac ggtattgatg ttttcagcca
      841 atcgtcctat cacactaatg gattccacgt cgtcctccat ctttacctgc agccactgca
      901 aggcattgtt gataaagtgc ggattcaact ggttgacgat ggcactcact ctcaaggtat
      961 ccttttcccg attcatcaat tcaaattcag tttctttctg agccagactc aatttctgac
     1021 gatagattgt tctttgtctg ctgtacaaat ataatcccag cagagtaaaa attataaaac
     1081 ttaccaacca aacctgccat ctgagccaca aagggggtaa aatggcgaac gaaatatttt
     1141 tgacaggaga ataattccca tctttgaccg cccggatctg aagattatag tcacctgaat
     1201 tcagattttg aaaaagaatc tgactttccc gctggagagg tgaccagtca tcattgttga
     1261 gacgatactg aaaactcaga ttgtgataga gtaaactatc caccggcacg ctaaagtaaa
     1321 ggctaagcgt acgttgctca ttggcaagtt ttattttgtc ctcaaatgag gtaaacaacc
     1381 tattgcccac cttcatactg tcaatgaaga catcccccgg agggtttgtc ggcttgtatt
     1441 taaagggatt gtggctgacg ataccatcga ccgtcaccag ccaataattc ccatcttttg
     1501 agcgaaggaa gctgttctgt tcaattgacc ccaatgtata attttcattt ttcattttaa
     1561 aagccccacg aagcggacag atttgtggat tggtataaaa cttattcagg tcaatcaaaa
     1621 agtatccttt tggatagcca actaaaagag tggagtcatc aactaattct atttcattaa
     1681 tttcatttaa ttcatttttt tcaaaaattt cctcccccac cttaatgcat tctttgcgca
     1741 tttcctgaat ggcttcgatg tcaggccggt ttttaagaaa gtataatcct gtcaatcctc
     1801 caaaccatag attccccctc ctgtcttcag caatggatat agcaccgatg tctttctttt
     1861 tatccctgat tagccaattg attactgtat ctttcccggg atcatacact gctattccct
     1921 gcgaggccct gcccatccag tacctgccat atctgtcctg aacagcagct aacacgacat
     1981 ccagttttaa tccctttttc ttgtcgatga ccttgtacag attgtcagag gtcaattttt
     2041 tatcatccct aaagatatac agaccatcag aaaatgtccc gaccatcaac ctgccatggc
     2101 ggtcccggtc aaagtaaatc cccaattttt tatccatcag ccacttccat ttcttatctt
     2161 ttacagccag gacgcccgtt gtaagaaact tagaagagtg cacattgaat agcatggtcc
     2221 cgtcatgcat ccggatggta gaattggcta actgagcctt acttaaatca ttaaaatttc
     2281 cgggtggtct tctgaactta tatccatcaa aaatagtcag accttcacca aagcttccca
     2341 gccaaagatt gctatcagaa gattgctgaa tagtccagac agaatttagt agtcctgtcg
     2401 tgcctaatgg gatagaatac tgatagcaca aaattttctg aacggcgcgt tccgtaccta
     2461 tccaaaagtt acccgcagca tctttggtaa caaaattggg ttt
//
LOCUS       DAOQMC010000304         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_25798_length_2497_cov_4.755528, whole genome shotgun sequence.
ACCESSION   DAOQMC010000304 DAOQMC010000000
VERSION     DAOQMC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25798_length_2497_cov_4.755528"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(520..1083)
                     /locus_tag="PKV31_09680"
     CDS             complement(520..1083)
                     /locus_tag="PKV31_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HNB93170.1"
                     /translation="MQNQSDNFLTSVIRLSEYYKSLGEKAMVQLEEEHLNLRLNEESN
                     SISTIVKHLWGNMLSRWTDFLNADGEKEWRERDAEFENDICGRHDIMAKWEEGWKCYL
                     DALRALSPDDLTKIVYIRNQGHTVQDAIHRQLAHYSYHVGQLVFLAKVLVNHPWESLS
                     IPRHGSIAYNAEKFSQEKRREHFTEDK"
     gene            complement(1103..>2497)
                     /locus_tag="PKV31_09685"
     CDS             complement(1103..>2497)
                     /locus_tag="PKV31_09685"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNB93171.1"
                     /translation="LRLAWAITIHKSQGLTFDRVMIDAAASFSSGQVYVALSRCKTLE
                     GIVLLSRIESRAILFDNRIDTGISRLNYEKRAQAYLQQARNNYILTIVGEMLGFHNEI
                     RYCNFILSELRKFGDKFPTDSGEYWIGVKSTLAEYQPIVDKFILSLEKLSKEDKQFEE
                     KATLKGRIHDAIKYFEPRVAGLVRQVKANPTNTESREAASRVNELLNELFDALARKQH
                     ILSRCNTAFKLELYQEAKKTFNPRHEKLSVYNQSSDADDEGNHPLLYKRLRRWRDSIV
                     QSNNIPVYMVGNSKMLQEIATLLPVEKKQLLNVKGMGSAKVAKYGEEILDIVRDYCDE
                     FDIEPAMPALTFEKKTKVDEPKKKVSEGKSIKQKTTEITYGLWRANPDINYLARLRNL
                     TTGTIEHHLSLLIQEGKIKIEEFVPPDMIRLLTPILARHEGKTLSEIKPFCPESVTFE
                     QLRALREHLNQGKS"
BASE COUNT          615 a          561 c          543 g          778 t
ORIGIN      
        1 cgaggttgag gcgattgacg aagagggcaa attcacccgt gatgagttgc agagacaatg
       61 tgacgccttt tttcattttt tcgcccttag tgaaagtgac ctggtcaatc aatcatacag
      121 tgacctgttg ttgaatacag catgacgtga acctaataat cagtaatctt catcgtctat
      181 ccatgaaagg ggatgcttcc tttacgatca aaacctgtat cagacaaatc ttccgatgac
      241 ctaatctccg ttcctgcttt tagccggatt agtgcgattt tataccagga tgatttatat
      301 aatagtccat aaatttgagg tatgcttgtc cggataacta tgctaccaga gctatgctct
      361 gtcccgggag tgaaacaatc cgggattcgg gatgccgagg ttacagtcgt tattagtttt
      421 agccttgtaa actaaaataa gaaaaacggt atcttggttt gtgtcgctat ttacagcctg
      481 taaaaatctt tcgttatgtg ccgtccttga agcaactccc tatttatcct ccgtaaaatg
      541 ttctcttctc ttttcctgag agaatttttc agcgttgtag gctatggatc cgtgccgtgg
      601 tatgctgaga gattcccacg ggtgattgac caggactttg gccagaaata caagctgacc
      661 cacatgatag gaatagtgtg caagctgacg atggatagca tcctgaaccg tatggccctg
      721 attgcggata tatacaattt tggtcagatc atccggagaa agtgcccgga gtgcatcaag
      781 ataacatttc cagccctcct cccatttggc catgatatcg tgcctgccgc aaatgtcatt
      841 ttcaaactct gcgtcacgtt cgcgccattc cttttctccg tcagcattca gaaaatccgt
      901 ccagcgtgaa agcatattgc cccaaagatg cttgacgata gttgatatgc tgttgctctc
      961 ctcattgagc ctgaggttga gatgttcttc ttccagttga accatggcct tctcccccag
     1021 agatttatag tattcagaaa gtctgatgac cgaagtaaga aagttatcac tttgattttg
     1081 catctgataa aacgttttag gttcatgatt tcccttgatt aaggtgttct ctcaatgcac
     1141 gcaattgctc aaaagtaaca ctttccggac agaatggttt gatctcggag agcgttttcc
     1201 cttcgtgcct ggcaaggata ggggtaagca atcgaatcat atccgggggg acgaattctt
     1261 ctatcttgat ttttccttcc tgtatcaaaa gggacagatg atgctcaatg gtcccggtag
     1321 tcaggttcct caacctggca aggtagttaa tatccggatt ggcacgccat aatccatagg
     1381 tgatttccgt cgttttttgt ttgatggatt taccttcgga gaccttcttt ttaggttcgt
     1441 caactttggt tttcttttcg aaagtcaaag cgggcatcgc tggttctata tcaaattcat
     1501 cacaataatc gcggacgatg tccaatattt cttctccgta ttttgccact ttggcacttc
     1561 ccatgccctt cacattaagt agctgttttt tttcgaccgg cagaagagtg gctatctcct
     1621 gcagcatttt actgttgccc accatgtata caggaatgtt gttgctttgc acaatactat
     1681 cccgccagcg tcgtagtcgt ttgtataaca aagggtgatt gccttcatca tccgcatcgg
     1741 atgactggtt atacaccgaa agtttttcat gtcggggatt gaatgttttt ttagcctcct
     1801 gatataattc gagcttgaat gcagtgttac agcgactcag gatgtgctgc ttacgagcaa
     1861 gtgcgtcaaa caattcattc agtaattcat tgactctcga agctgcttcc ctggattcgg
     1921 tattcgttgg attggccttt acctgccgaa ccagccctgc aacccttggc tcaaaatatt
     1981 ttatggcatc atggatacgt cctttcagtg ttgccttttc ttcaaactgc ttgtcttcct
     2041 ttgatagctt ctccaggcta agaataaact tatctacaat cggctgatat tctgccagtg
     2101 tactttttac cccaatccaa tattcgccgg aatctgttgg aaacttgtca ccaaacttac
     2161 gcaattcgga gaggatgaaa ttacaatatc tgatttcatt atggaagccg agcatttctc
     2221 ccactatggt aagaatataa ttattacgag cttgttgtag gtatgcctga gcccgttttt
     2281 catagttgag tctactgata ccggtatcga tgcggttgtc gaagaggatg gcgcgcgact
     2341 caattctgga aagcaggaca atgccttcca gtgttttgca tcgactgaga gccacataga
     2401 cctgaccact ggaaaatgat gctgctgcat cgatcattac ccggtcgaag gtcaggccct
     2461 gacttttatg gatcgtgatg gcccaggcaa gcctgag
//
LOCUS       DAOQMC010000305         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_25952_length_2489_cov_5.634758, whole genome shotgun sequence.
ACCESSION   DAOQMC010000305 DAOQMC010000000
VERSION     DAOQMC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25952_length_2489_cov_5.634758"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKV31_09690"
     CDS             complement(<1..411)
                     /locus_tag="PKV31_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93172.1"
                     /translation="MTGSGRTSTNYHYLCTYLYGILMQKQVLEAEQKALEINLDPSIY
                     GTFAEIGAGQEVARYFFQAGAAAGTIAKTMSAYDKTYSDEIYGAEPSGRYVCESRLYK
                     MLDHEFKLLVDRLGKIRPDTTFFAYADTVSATNYA"
     gene            1191..2243
                     /locus_tag="PKV31_09695"
     CDS             1191..2243
                     /locus_tag="PKV31_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93173.1"
                     /translation="MSFTKTSTSNLSGKLSGKSIGMGSKGQPWYMNKVFLGSALFAPV
                     GGLIGIVAAKSLDDNSPLSTEGKTATTIAVDKPEETIAVASAPAENAANIIPETESEE
                     MHINFISDLPCGHPSDNLSFGEAFREQRNILGQGGVFEYQGKYYNTYYKEEWDAMTDE
                     QKNEYFLSIDSKIDYSKSFITGHHKGNQYEVSVQDFNQSAFSNHNSPFIPEDQAEGYV
                     PFEYGHTPMEAGPNAIHLQPDFEVPSTVIVDDYDFNSGSHSFNGEIDHSYFNYDNSIG
                     LNDDLELQASIVGSDDSILDVEPGDLAHHFDYNQYGNSDGAGHETIYFDHDMDTPDSL
                     DENGHHEHGYTFPDDF"
     gene            2272..>2489
                     /locus_tag="PKV31_09700"
     CDS             2272..>2489
                     /locus_tag="PKV31_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93174.1"
                     /translation="MELKCPFCDERIILANIEKYVGKQIRVKCVNKSCQQYFVTRVPD
                     SSDKTIVFSAGSVKTRAKLTVLASKFAP"
BASE COUNT          768 a          510 c          494 g          717 t
ORIGIN      
        1 ggcataattg gtggcggaaa ccgtgtcggc ataggcaaaa aatgttgtgt ccggccgtat
       61 ctttcccaac cgatcaacca gcaacttaaa ctcatggtcc aacatcttgt acagtcggga
      121 ctcacataca taacgaccac tgggttctgc gccatatatt tcatcggaat aggtcttgtc
      181 ataagcagac atggtcttgg cgatggtgcc tgcagcagca ccagcctgaa aaaagtatct
      241 ggcgacctcc tgtcctgcgc ctatctcagc aaaggtgcca taaatacttg gatcaagatt
      301 gatctcaagc gctttttgtt cggcttctaa tacttgtttc tgcatcaata ttccgtaaag
      361 ataggtgcaa agatagtgat aattggttga agttctgcct gatcctgtca cgcacgatat
      421 tcacaaaaaa taaggacatt agtaagacac gaaacccgtg ttgttccctc cttgtactgc
      481 gcttactatt gaataggaca atctcgtcct ggacttattc tcccggacaa tttgttacaa
      541 ccggtagtgt ggaataatcg atgaataacg accaaaatat cgcgttttac aaagccttca
      601 gcattggttt taccaattag ataacaaatt ttactgagaa acaaaaaacc aaagctatgt
      661 agaattatta atgtttcttc gacggtgaat tctacaaggc cggtgcccca aagagcttac
      721 ttttggcgga tgccgggttc ttcatttgtg cattttgtta ttattacgtt gaattctgat
      781 ctgacattct taattcatgc tgaagacaaa agatgggttc cttaaattta tggtgttttg
      841 tcagacactg ataaaacacc ataaatatcg aacaaatcaa tttattttac tgcatcacac
      901 attttgaaag ttatccgcat tccatcttta ttattcgaaa agggcacttt gtgatttaca
      961 cccggttagc cgatatctgt aacaaaagac ttaggattca aataaaaatt agcaatgccg
     1021 tgtcttttcg ggccttcaat aaatttcaat ttgaataaat accccggtca taatcttcaa
     1081 aatcacggat tcatacaaat taagctttga ttcatacata tttaaagcct ggattaggga
     1141 tagaatatta attttaagcg agaattatta aagcattaaa ccaatacagt atgtcattta
     1201 caaaaacatc tacatcaaat ctttcaggca aattgtccgg aaaatccatt ggaatgggat
     1261 caaaaggaca accttggtat atgaataaag tttttctggg gtcagccctg tttgctccgg
     1321 tcggaggctt aatcggtata gtagctgcca agtccctgga tgacaactcc ccattatcaa
     1381 cagaaggaaa aacagcaaca accatcgcag tagataagcc ggaagaaacc atcgcagttg
     1441 cgagcgcccc ggctgagaat gcggcaaata ttatacccga aaccgaatcg gaagaaatgc
     1501 atatcaattt tatctccgat ctgccttgtg gacatccgtc cgacaacctt tctttcggag
     1561 aagcattccg tgaacaaaga aatattttgg gtcagggggg cgtatttgaa tatcaaggca
     1621 aatattataa cacctattac aaggaagaat gggatgctat gacggatgaa cagaagaatg
     1681 aatatttcct gtcaatagat tcaaagattg attattcaaa gtctttcata acaggccatc
     1741 acaaaggaaa tcaatatgag gtgtccgtac aggatttcaa ccagagtgcc ttttctaatc
     1801 ataattcacc attcattccc gaagaccagg ctgaaggata tgtgcccttt gaatacggtc
     1861 atacacccat ggaagccgga ccaaacgcca ttcatcttca acctgatttt gaggttccct
     1921 ccacagtaat agttgatgat tatgacttta attccggctc acactcgttc aatggagaaa
     1981 tagaccattc ctacttcaat tacgataatt cgataggatt gaatgatgac ttagagcttc
     2041 aggcttcgat cgtaggaagt gacgatagca tcctggatgt agagcccggt gatttggcac
     2101 accatttcga ttataatcaa tacggtaatt ctgatggcgc cggtcatgaa acaatatatt
     2161 tcgatcatga tatggatact ccggattcat tggatgaaaa tggccatcat gaacatggat
     2221 ataccttccc ggatgatttt tgatatacgg ataacctcgt ggtattgaag tatggagttg
     2281 aagtgtcctt tttgtgatga gagaattata cttgccaata tcgaaaaata tgtagggaag
     2341 caaatccggg tcaaatgtgt caataagtca tgccagcaat actttgttac cagagttcct
     2401 gattcatccg acaaaacaat tgttttttct gccggatctg tcaagacgag ggcaaagctg
     2461 accgttcttg ccagtaaatt cgccccttt
//
LOCUS       DAOQMC010000306         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_26131_length_2479_cov_4.688944, whole genome shotgun sequence.
ACCESSION   DAOQMC010000306 DAOQMC010000000
VERSION     DAOQMC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26131_length_2479_cov_4.688944"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKV31_09705"
     CDS             complement(<1..721)
                     /locus_tag="PKV31_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB93175.1"
                     /translation="MLRSDKYISINPYTGNLIREFPYWDGKQIDQSIRSSKQAYHFWS
                     HTTIEERKSFLAALSEFLKLKADDWAVIITEEMGKPLSQSKAEILKSATLCDYYYQQL
                     EVLSQPRMIPLDNKRYGMVKPQAQGAVLGMMPWNFPIWQAIRFAVPVISGGNVVLLKH
                     AQNVGITANLIEEAITHAAGLEGVLINTFITHDDIARYLQDEHIVGASLTGSERAGVA
                     VGRIAGINIKKTVMELGGSDVF"
     gene            complement(705..2015)
                     /locus_tag="PKV31_09710"
     CDS             complement(705..2015)
                     /locus_tag="PKV31_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB93176.1"
                     /translation="MRLRSFIVLAITLILVSCKSTTRVVYPEKKDKTSIPEIKNPERP
                     DSTHHSKIVKQNKKDSVGNEVIKPDANPVVIPTEKTKPNKHLKNNIKVAVALPMKGGY
                     DVSRFVEFVNGMSLAGSLSGGTSTDRLDIHFMDIGNDNSAEKLASIKAILDADIILGG
                     YQTSQVKALAEIALSKAIPYISLWNSSDDIVNHNPYFFQMKPSLEAYCSNMASYITKQ
                     LRPDLTIILLEGKNTKDASTIDYFSNIYQKERINFKVVYGLGSVDWQTLFMNYNRIVV
                     NIPNWDERQFVKKALKQLIEAKKNKNLTVTGMPQWASWDQADFTLFENCGLLVPIATS
                     VDLNAPEVIIFQKNYFEKYGTWPTSESFYGNDILYLIKTISSNLMKGDSFEPVNGYSA
                     KYFSSYEFRPVVTRPGGEVPQLSYYRNQSITIEKFEGGRFVTVR"
     gene            complement(2054..>2479)
                     /locus_tag="PKV31_09715"
     CDS             complement(2054..>2479)
                     /locus_tag="PKV31_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB93177.1"
                     /translation="RRVGIALGLQPQFVHRHPFPGPGLGVRILGDITAEKVRILQDAD
                     DIFISGLRKSGLYDQVWQAGVVLLPVRSVGVMGDERTYENVVALRAVSSTDGMTAEWS
                     RLPHDFLADISSEIINKVKGVNRVVYDVSTKPPATIEWE"
BASE COUNT          678 a          534 c          471 g          796 t
ORIGIN      
        1 tgaaaacatc gctgcccccc agttccatca ccgttttttt aatgttgata ccggcgatgc
       61 gtcctacagc tactcctgcc ctttcgcttc ccgtcaggga tgcccccacg atatgttcat
      121 cctgtaagta ccgggcaata tcatcgtgag taataaaggt attgataagt acaccttcca
      181 ggcctgctgc atgggttatc gcttcttcaa tcaaattagc tgtgattcca acattctgtg
      241 catgtttcag caggacaaca tttccaccgc ttatgaccgg tactgcaaag cgtatagctt
      301 gccaaatcgg gaaattccac ggcatcatgc cgagtactgc cccctgagcc tgtggtttga
      361 ccataccata gcgtttattg tcaagcggaa tcatgcgagg ctgtgacagt acttccaact
      421 gttggtaata ataatcacac agtgtggcgg atttaaggat ttcagctttg ctctgggaca
      481 atggtttacc catttcttcg gtaatgatta ctgcccaatc atctgctttc aatttcaaaa
      541 attcagacaa agctgcaagg aaggactttc gctcttctat ggtagtatgg ctccagaaat
      601 ggtatgcctg tttggaggat cttatagatt gatctatctg cttgccgtcc caatatggaa
      661 attctcttat taggtttcct gtataggggt tgattgaaat atatttatct gaccgtaaca
      721 aaacgccctc cttcaaattt ttctatcgtt atggactgat ttcggtaata ggacaattgt
      781 ggtacttctc ctccaggtct ggttactaca ggtctgaatt cataggatga aaaatattta
      841 gcagaataac catttaccgg ttcaaaggaa tctcctttca tcaagtttga ggaaatagtt
      901 tttatcaaat aaagtatatc atttccataa aaggattctg aagtaggcca tgttccatat
      961 ttttcgaaat aatttttctg aaatataatg acttcaggag cattaagatc aacactagtt
     1021 gctatgggca ctaacagtcc gcaattttca aatagagtaa aatcagcctg atcccagctt
     1081 gcccattgag gcataccggt aacagtcagg tttttatttt ttttagcctc aattaattgc
     1141 ttcaatgctt ttttgacaaa ttgtctttca tcccaattag gaatattaac aactattctg
     1201 ttataattca taaaaagtgt ttgccaatcg acacttccaa ggccatagac taccttaaaa
     1261 tttatccttt ctttttgata tatgttcgaa aagtaatcta tcgttgatgc atccttagta
     1321 ttctttccct caagcaaaat aatggtcaaa tcaggcctca attgctttgt aatgtagctg
     1381 gccatgtttg agcaataggc ttccaatgaa ggtttcattt gaaaaaaata cggattgtga
     1441 ttaactatat catctgaact attccagagt gaaatgtagg gtattgcttt cgaaagggct
     1501 atttcagcta aagcttttac ctgactggtt tgatatccac ccaggatgat atctgcatcc
     1561 aggattgcct taatagatgc cagtttctct gcactgttgt catttccaat atccataaaa
     1621 tggatgtcta gtctatccgt tgatgttcca ccggataaag acccggcgag gctcatacca
     1681 ttgacaaact caacaaaacg acttacatcg taccctcctt tcatgggaag ggcgacagca
     1741 accttgatat tattttttaa atgtttattc ggtttggttt tttcggtagg tattaccaca
     1801 ggattagcat ccggcttaat aacctcattg ccaacagaat cttttttatt ttgtttgact
     1861 attttggaat ggtgtgtact atcgggacgc tcaggattct tgatttccgg tattgatgtt
     1921 ttgtcctttt tttcaggata aactaccctg gtcgttgatt tacaactgac taaaattaag
     1981 gtaatagcaa gtacaataaa tgaccgtaac cgcatatcgt tgtctgaatt ttcgtgggac
     2041 tatagcgaaa cagttattcc cactcaattg ttgcgggtgg ctttgtactg acatcgtaaa
     2101 ctactctgtt aactcctttt actttattga tgatctcaga agatatatcg gcgaggaagt
     2161 catgcggtaa tctactccat tcagcagtca tgccgtcggt acttgaaaca gcccgtaatg
     2221 ccaccacgtt ttcgtaagta cgttcatcac ccataacccc gacagatctg acaggcaata
     2281 atacaacgcc tgcctgccag acctgatcat agagtcctga tttgcgcaag ccgcttataa
     2341 agatatcgtc agcgtcctga agtattctaa ccttttccgc agtaatatct cctaatattc
     2401 taactccgag accaggtccc gggaagggat gcctatgtac aaattgtggt tgtaagccta
     2461 atgcaatgcc cacacggcg
//
LOCUS       DAOQMC010000307         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_26474_length_2461_cov_3.494181, whole genome shotgun sequence.
ACCESSION   DAOQMC010000307 DAOQMC010000000
VERSION     DAOQMC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26474_length_2461_cov_3.494181"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="PKV31_09720"
     CDS             complement(<1..1574)
                     /locus_tag="PKV31_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNB93178.1"
                     /translation="MHKIVLWILLLLCSWQAFGQDLPRGFAEGEKELMPQYIRTATSY
                     NPFMRGVDVPGPIRSMAEWEELQGLIISWTENFRDIQAEIVRSTIPQCRIIIACSNPD
                     NVSKFLASKGISDSLNVQYVVGRYNSIWVRDYGPNSVYYNDVDSLYLTDWIYNRPRYQ
                     DDTLARQLSRALNLPLMETNKKPDDLVHTGGNYMSDGQGTAFSSLLVMDENGIGNTFG
                     FSIHTEPEVDSLMAKYMGTKVYAKMETLPYDAIHHIDMHMKILDEQTILVGKYANDVA
                     DGAQINANIAYLLDHYKNYFGKPFTIKYIPMPPGPNGRYPDQNGDYRTYTNSVFVNKT
                     VIVPFYEEKYDTIARRIYEEALPGYQIVGINCNKIIPSLGAIHCITKEVGVTDPLLIS
                     VDIAQPIINPDNERERTIHAIVKNKCGIDEVWLHFTLDGSHDTTDSLKMELTDSEKSI
                     YRAIIPTFKKEARYYITAKSISGKTISRPMTAPEGYFKTVAIPTASTRTNTPQRMHIF
                     PNPARSLTCVDVDYPMA"
     gene            complement(1591..>2461)
                     /locus_tag="PKV31_09725"
     CDS             complement(1591..>2461)
                     /locus_tag="PKV31_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB93179.1"
                     /translation="IGLKYNANDRLRFKGAAGMYSQNILSTVDDRDVVNLFNGFLSGP
                     DESIYDFGKNEQLNDVLQKAWHAVVGVEYDLNQYLELNIEPYYKDFTALLNLNRGKSK
                     ANDPNFILETGNAYGIDLTAKYSRGRYYFWGTYSYSKVTRDDGRQIYPTIYDRTHNIN
                     LLGSVAFGKERSFELSGRFNFGTGFPFTRALGFFEQIPATDLSTDFASTNGQIGIIYE
                     SKRNGGRLPDYARFDLSAKKTFKFKEHLRLEVNASVSNVTNRDNIFYVNRLTYERINQ
                     LPILPSAGMNLFF"
BASE COUNT          665 a          512 c          518 g          766 t
ORIGIN      
        1 gccatcgggt aatccacatc tacacatgtc agggatctgg caggatttgg gaaaatatgc
       61 attcgttgag gcgtgttggt ccttgtcgaa gcggttggga tggcaacagt tttaaaataa
      121 ccttccggtg cagtcatagg cctgcttatg gtttttccgg aaattgattt ggctgtgata
      181 taatatctcg cttccttttt aaatgtaggg ataatggctc tataaataga tttttctgaa
      241 tctgtcaatt ccattttaag gctatcggtt gtatcatgac ttccatctaa tgtgaaatgt
      301 agccacacct cgtcgatgcc gcatttgttt tttacaatgg catgaatagt ccgttccctt
      361 tcattgtcgg ggttaatgat tggttgggct atatccaccg aaatgagcag cgggtcggtg
      421 acgcccactt ccttggtgat acaatggatt gcacccaggg agggtataat tttattacaa
      481 ttaataccta caatttgata tccgggcaat gcctcttcat atattcgcct ggcaattgta
      541 tcatattttt cttcataaaa tgggacaatg actgttttat ttacaaacac agaattagta
      601 taagttctgt agtccccatt ttggtcagga tatcgaccat ttggcccggg tggcataggt
      661 atgtatttga tggtgaatgg tttaccaaaa taattcttat aatgatccag taagtaggct
      721 atattggcgt tgatttgggc gccatccgca acatcattgg cgtacttacc taccaggatg
      781 gtttgttcat ccaaaatttt catgtgcata tcgatatggt gaattgcgtc atatggcaag
      841 gtttccattt tggcatagac tttggttccc atgtatttcg ccataagaga atcaacttca
      901 ggttccgtat gaatggaaaa gccgaaggta ttgccgatgc cattttcatc catcaccaac
      961 aaggaagaaa aagctgtgcc ctgtccatct gacatataat tcccacctgt atggactaag
     1021 tcatcaggtt ttttgttggt ttccatcaat ggcaggttga gtgccctgct caattgtcgg
     1081 gccagtgtat cgtcctgata ccgcggccgg ttataaatcc aatcagtcag atacaacgag
     1141 tcgacatcat tataatatac cgaatttggt ccataatccc gaacccagat cgaattatac
     1201 cttcctacta catattgtac attgagactg tcgctgattc ctttggacgc gaggaattta
     1261 cttacattat cggggttaga gcaagcaatg ataatcctgc attgtggaat ggtactgcgc
     1321 actatttctg cctgaatatc gcggaaattt tctgtccatg aaatgatcag accttgcagt
     1381 tcttcccatt ctgccatact tctgattggt ccggggacgt cgacaccccg catgaagggg
     1441 ttgtacgaag ttgccgtacg aatatattga ggcatgagtt ctttttcacc ttctgcaaat
     1501 ccacgcggca aatcctgacc aaaagcttgc catgaacaca gaagtaatag gatccaaaga
     1561 actattttat gcatggcaaa tgatatcttg ttaaaagaag agattcatac ctgcactagg
     1621 cagaataggc aattgattga tcctttcgta ggttagcctg ttgacataga atatattatc
     1681 tctgtttgtt acattcgata cacttgcgtt aacctccagt cgcagatgct ctttaaattt
     1741 gaatgttttt tttgctgaaa ggtcgaatct tgcataatcc ggaaggcgac ctccatttcg
     1801 tttgctttca tagattatgc cgatctgtcc attggtagaa gcaaagtcgg tacttaggtc
     1861 ggtagccgga atctgttcaa agaatcccaa ggcacgagtg aatggaaaac cggttccaaa
     1921 attgaacctt ccgctaagtt caaacgatct ttcctttcca aaagcaactg atcctaagag
     1981 attgatattg tgggttctgt catagatcgt ggggtatatc tgacgaccat catccctggt
     2041 aaccttactg taagaatagg taccccaaaa gtaataccgg ccacggctat atttagcagt
     2101 caggtcaatt ccgtaagcat tcccggtttc taatataaaa tttggatcat ttgccttgga
     2161 tttcccccga ttcaaattga gcagggctgt aaaatcttta taataaggct caatatttag
     2221 ttcaaggtat tgattgaggt catattcaac cccaacaaca gcatgccagg ccttttgcag
     2281 tacatcgtta agttgttcgt ttttgccaaa atcatagatt gactcatcag gaccggaaag
     2341 gaagccgttg aaaaggttga caacgtccct atcatccact gtactgagaa tattttgaga
     2401 atacataccg gcagcgccct tgaacctaag tctatcgttg gcattgtatt tcaatccaat
     2461 c
//
LOCUS       DAOQMC010000308         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_27384_length_2415_cov_3.240254, whole genome shotgun sequence.
ACCESSION   DAOQMC010000308 DAOQMC010000000
VERSION     DAOQMC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27384_length_2415_cov_3.240254"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1453)
                     /gene="rlmD"
                     /locus_tag="PKV31_09730"
     CDS             complement(32..1453)
                     /gene="rlmD"
                     /locus_tag="PKV31_09730"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNB93180.1"
                     /translation="MGRKRKGFKSIIFENREITGIAEESRSVCRTDDGIVCFVEGAVP
                     GDVVDVLVTQKKKGFYFGAVHNLVEASPDRIKAPCKHFGICGGCKWQHFHYAGQLREK
                     ENKVLQAMLRIGKTEPQEFQPILGCGEEFFYRNKLEFTFSNKRWLEPEEMSSEGDILK
                     EPGLGFHRAGAFDKVVDIEECMLQPDPSNAIRNFVKAFAIRMNWTFFNVRQREGFLRN
                     LMVKTSSIGEVMVLLSFHYKDDEAIGVLMEAIMTNFPEITTLVYVINEKANDTFLDLP
                     IEIYHGPGYIVEKLGEVKYRLGPKSFFQTNTNQAVRLYDLALEFAGLQPTDHVYDLYT
                     GLGSIALYASSRCKSIVGVENVAPAIEDARANADWNGISNATFVVGNVEDVVDTGFIT
                     KYGRPDVVITDPPRAGMHERVIDTLLQLECPKIVYISCNPSTQARDVAMLSTKYNLEK
                     LRAVDMFPQTNHIESVALLTLKK"
     gene            complement(1466..>2415)
                     /locus_tag="PKV31_09735"
     CDS             complement(1466..>2415)
                     /locus_tag="PKV31_09735"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNB93181.1"
                     /translation="TADNYNTLEPISPFLNELTSLFFKRNYIKFYDNTYIRLSGMHQV
                     SPGFSLIGSVRAQHREGLLNHTDYSFRLKDENYQFNYPEEWSLENFYKKSNAYTTSLT
                     LRWQPGNKIIKLPERSFILGSSLPVVDLSLINAWAFSASDARYNRVNLEVSNIKVALG
                     ALGTITSNIHGGLFYGKRPQYEMDYAHFPGNLVYVKNTNSYIDAFKRFSYYKYSTPDK
                     YLTMFAEWNLKGFLFKKIPLLDKTGFEEVFAINAMATPETGSRIEVSAGIDRIGYSVF
                     RGVRFDYIWIFENGKYLTDNFVIGLNLDAITSLFKGISQ"
BASE COUNT          671 a          573 c          483 g          688 t
ORIGIN      
        1 cttgatttca ggatttagaa agtgattccg tttatttctt cagggtcagc aaggcaacag
       61 actctatatg attggtctga ggaaacatgt ctactgcccg caacttttca agattgtatt
      121 tagtgctcag catggcgaca tcacgtgcct gagtggatgg attgcagctg atgtatacta
      181 ttttagggca ttccaattgc aagagggtat ctattaccct ttcatgcata ccggctctgg
      241 gagggtcggt aatcacgaca tccggtcttc catattttgt tataaatccg gtatccacca
      301 catcctcaac attaccaacc acaaaggtgg cattggagat accattccaa tctgcattgg
      361 ccctggcatc ttctatggca ggggcgacat tctcgacacc tactatactt ttgcaacgtg
      421 acgaagcata cagggcgata ctgcccaaac ctgtatagag gtcataaaca tgatcggtgg
      481 gctgcagccc ggcaaactca agggccagat cgtataacct tacagcctgg ttagtattcg
      541 tctgaaaaaa tgacttaggg cctagccgat atttgacttc acccagcttc tccacgatat
      601 aacccgggcc gtggtaaatc tcaataggaa gatccagaaa agtatcattc gccttttcat
      661 tgatgacata taccagcgtt gtaatttcgg gaaaattagt catgatagcc tccataagca
      721 ccccaatcgc ttcatcatcc ttgtaatgga aagaaagtaa caccatcact tcgccgatgg
      781 aagaggtttt aaccatcagg ttacgcagga agccctctct ttgtctgacg ttgaaaaaag
      841 tccagttcat ccggatggca aatgctttga caaaattgcg aatggcattg gatggatcgg
      901 gttgcaacat acattcttcg atatcgacaa ccttatcaaa ggctcctgcc cggtgaaaac
      961 caaggccagg ctcctttaag atatcaccct cactactcat ttcctccggc tctaaccagc
     1021 gtttattaga gaaggtaaat tccagtttgt tacgatagaa aaactcttct ccgcaaccca
     1081 gtatgggctg gaactcctga ggctctgtct taccgatacg caacattgcc tgcaaaacct
     1141 tattttcctt ttcacgtaat tgtcccgcat aatgaaagtg ctgccatttg cagcccccgc
     1201 aaattccaaa gtgtttgcag ggtgccttta ttctatccgg tgatgcttca accaaattat
     1261 ggactgctcc gaaataaaag cctttctttt tctgtgtaac caggacatcc actacatccc
     1321 ccggaactgc cccttcaaca aagcaaacga ttccgtcatc ggtcctgcat accgatctgc
     1381 tttcttctgc aatacccgta atctcccggt tttcgaaaat aattgattta aatcctttac
     1441 gttttctacc cataacagag attacttatt ggctgattcc tttaaacaaa cttgtaatgg
     1501 catccagatt caatccaatt acaaagttgt cggtcaggta cttgccattt tcaaaaatcc
     1561 agatataatc aaatctaacg ccacggaata cggaatatcc aatcctgtca attcctgcac
     1621 tcacctcgat tctggatcct gtttccggag tcgccatcgc attgattgcg aaaacctcct
     1681 caaaaccggt tttatccaac aatggaattt tcttaaaaag aaagcccttc agattccatt
     1741 ccgcaaacat ggtaagatac ttatcaggcg tactgtactt ataataggaa aaccgcttaa
     1801 aagcatcgat ataagaatta gtattcttaa catacaccag gttgccgggg aaatgagcat
     1861 aatccatttc atattgcggg cgtttgccat agaaaaggcc tccatgtatg tttgatgtta
     1921 ttgtgcccag ggcacccagg gcaactttga tattacttac ttccagattg acccgattgt
     1981 atctggcatc acttgctgaa aaagcccagg cattgatcag ggacaaatct acaaccggca
     2041 gggaagaacc aaggatgaag gacctttcgg gcagcttgat gatcttgttg ccgggctgcc
     2101 atcttaatgt cagggatgtg gtatatgcat tactcttctt atagaaattt tcaaggctcc
     2161 actcttccgg gtaattaaac tgataatttt catccttgag tctgaagctg taatctgtat
     2221 ggttgagcag cccttcccgg tgctgggctc taacagaccc gattaaggaa aagccggggc
     2281 tgacctgatg cattccggaa agcctaatgt aggtattgtc gtaaaatttt atgtaattcc
     2341 ttttaaagaa cagactggtc aattcattca aaaaaggtga gatcggttcc agtgtattgt
     2401 aattatctgc tgtgt
//
LOCUS       DAOQMC010000309         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_27765_length_2396_cov_4.908586, whole genome shotgun sequence.
ACCESSION   DAOQMC010000309 DAOQMC010000000
VERSION     DAOQMC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27765_length_2396_cov_4.908586"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2396)
                     /locus_tag="PKV31_09740"
     CDS             complement(<1..>2396)
                     /locus_tag="PKV31_09740"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNB93182.1"
                     /translation="TAIIPGFKAPEALKKLGTINYSGKLDGYILDFVTYGKVKTAQGD
                     ANVDIHLDIRDGAENAKYSGIIGLSQFNLGNLTGNQDLGKVTGTFTIKDGQSFSLESI
                     STELSTEVQSFTYKGYDYKNFKIDGKLLKNTFDGKLVISDPNANLKFDGAIDFSKDRK
                     VLNFNSEIYNLDLSKLNLANLSWKIKGTINSNLTLSDLSDMEGSFIAQNLDIKDTSGH
                     HLELDYLNFNAIKSGNGLKEYTLTSNMVDLDLIGKFEIEQIGQALIKVFHYNHTALAN
                     QLGIQSRVQNIYNNDFNFNLNVKDSKELLNLLGTSLQPVQGSRISGYFSNIDSTKYRI
                     NINGNIPVLRSDNFNFNYLFFEGIGNQESSEYFIFANSGNILDRSLSQMDLSATLIVD
                     SVRFNIKTPQIQKIAENLSIAGIYTVENGYNILKFNQSKFDFFDDGWTVNKDNLVKFG
                     KNELYLKNLTATNGRQEVIFNSYGKQGLEVNVKNFNAAVLDSLLANADLKIIGTGDIN
                     LRMDSVFTQQNILLTSHFDSVNVNGIALGKLNLEANAQSLKSKVGFDLSMGELNKELH
                     VTGLYTLPQYEAFDYPPNYIDLLISSESYPLVIADIFLGEMIKDTRGTFTTNFRIKGK
                     TNQLDLNGTALVQNMGTTIRYLGTRYYVPKHTVRINNNMIDLDGLTLLDERGNIASVS
                     GGIKHNRFKNLNADIEINSDNFLMLKTTEKDNPNYYGTASGRFTGKFKGPFDQLNIEI
                     EATTGNGTDLFLPIGTSKEVDALSFIEFKNRFKEDVEKEINKVVTSKGLAFKMNAEVN
                     "
BASE COUNT          659 a          458 c          443 g          836 t
ORIGIN      
        1 cattcacttc ggcgttcatc ttgaaggcca ggccttttga tgttacgact ttattgattt
       61 ctttttccac atcctcctta aatctatttt taaattcaat aaaggaaagt gcatctactt
      121 cctttgatgt ccctattgga aggaatagat ccgtaccatt gcctgtcgtt gcttcgattt
      181 caatattgag ctgatcaaaa ggccctttaa acttacccgt aaaacgtccc gaagctgttc
      241 cataataatt gggattgtct ttttcagtag tcttcagcat gaggaaattg tcggaattaa
      301 tttcgatatc ggcattaagg tttttaaatc tgttgtgttt gattcctcct gatacagatg
      361 caatatttcc tcgttcgtcc aatagggtca agccatccag atctatcatg ttgttattga
      421 tccggacggt atgtttaggt acataatatc ttgtcccaag gtatctgatg gtcgtaccca
      481 tattctgcac cagagcagtt ccattcagat cgagttgatt tgttttgcct tttatcctga
      541 aattagtggt aaatgttccc cgtgtatctt ttatcatttc acctagaaaa atatctgcaa
      601 tgacaagagg atatgattca gaacttatca gcaggtctat ataattcggg gggtaatcga
      661 atgcttcata ttgtggcaag gtgtaaaggc ctgttacatg tagttcttta tttaattctc
      721 ccattgacag atcgaatcct actttgcttt tcagtgattg tgcatttgct tcgaggttca
      781 atttgcccaa tgctattcca ttgacattta cgctgtcaaa gtgggaagta agcagaatat
      841 tttgttgagt gaatacactg tccatcctaa ggttaatgtc tcctgttcca ataatcttaa
      901 gatcagcatt ggctaataaa ctatccaaca ccgctgcatt aaagttcttg acatttacct
      961 ccaatccctg tttgccataa ctattgaaaa tcacttcttg tctgccgtta gtagccgtta
     1021 ggtttttcaa atacaattcg ttcttcccga atttcaccag gttatcctta ttgactgtcc
     1081 agccatcgtc aaagaaatcg aattttgatt gattgaattt taaaatatta tagccgtttt
     1141 ctacagtata gatacctgcg atacttagat tttcagctat tttttgaatt tgaggtgttt
     1201 taatattgaa ccggacgctg tccacaatta atgtcgcact taagtccatt tgactaagag
     1261 atctgtccag aatattgccg ctatttgcaa agatgaagta ttcagaggat tcctgattgc
     1321 caataccttc gaaaaaaaga tagttgaagt taaaattatc ggaacgtaag accggtatat
     1381 tcccattgat gttgatccgg tatttagtag aatcaatgtt ggagaaatag ccggaaatcc
     1441 ggcttccctg aacaggttgc aacgatgtgc ctaatagatt tagaagttct tttgaatcct
     1501 tgacattaag attgaaattg aaatcattgt tgtatatgtt ctgcaccctg gattggatgc
     1561 ccaactggtt ggccaatgcg gtatggttgt aatgaaagac ttttatcaga gcttgtccga
     1621 tttgttctat ttcaaacttg cctatgaggt ctaaatcgac catgttggaa gtaagggtat
     1681 attcttttaa accattcccg cttttaatag cattgaaatt gagataatcc aactcaagat
     1741 gatgaccgga ggtgtcctta atatctaaat tctgagcaat aaaacttccc tccatatcac
     1801 ttaggtcgct cagtgtcaga tttgagttta tggttccttt aattttccag cttaaattgg
     1861 caaggtttaa ttttgaaaga tcaagattgt aaatttccga attgaaattc aataccttcc
     1921 tgtctttgga aaaatcaatg gcaccatcga attttaaatt agcattggga tcggaaatga
     1981 ccaattttcc gtcaaaagta tttttcagaa gctttccgtc aattttaaag tttttgtagt
     2041 cataaccttt ataggtaaaa gattggacct cggtagacaa ttctgtactg attgattcca
     2101 gtgaaaagct ctgtccatct ttaattgtaa aggttccggt gacttttcca aggtcctgat
     2161 tgccagtcaa gtttccaagg ttgaattggc ttaatccgat aattccactg tattttgcat
     2221 tttctgcacc atccctaata tcaagatgaa tatccacatt ggcatccccc tgcgcagttt
     2281 ttactttgcc ataagttaca aagtcaagga tatagccatc gagttttcca ctatagttga
     2341 tggtgcccag ttttttcagg gcttccggtg ctttaaatcc gggaatgatt gctgta
//
LOCUS       DAOQMC010000310         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_28140_length_2379_cov_3.409208, whole genome shotgun sequence.
ACCESSION   DAOQMC010000310 DAOQMC010000000
VERSION     DAOQMC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28140_length_2379_cov_3.409208"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKV31_09745"
     CDS             <1..573
                     /locus_tag="PKV31_09745"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNB93183.1"
                     /translation="INYFEEGWFHIMSLDALDHQLFIISLAALFLLKDWRRVLVLVTA
                     FTIGHSLTLALSAYDIIRFSTAWVEFLIPITIVITALYDLTYTNLSKIEKRNINFNYI
                     IALSFGLIHGMGFANSIRMMLASDQRIGMPLLSFNLGLEAGQIVLILLILLAAQLIVV
                     NMKIPRKYWIILLSVIPLVLSFKMALERIP"
     gene            complement(601..1302)
                     /locus_tag="PKV31_09750"
     CDS             complement(601..1302)
                     /locus_tag="PKV31_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93184.1"
                     /translation="MTKLFVLISKQRHTFRRQRFFLAAIWMLEAFLCFSQQSDSMRIY
                     TQVDHMPQLVSCASGYGNMYKLDRCTVASLKAWMLANMQYPATALESGREERYSFRAL
                     VDTTGQIKEIKPAQDLSSECGNEANRLTKLMLQSGEKWTSGRQGTRKVPVWIEFCIEF
                     NAKDWQNELTRRANPQAFVSRDSLALKPVKPEMPKAKSKVKPKSNSKAGNKAKKNSSS
                     KSKYSKKKTSTKKKR"
     gene            complement(1360..>2379)
                     /locus_tag="PKV31_09755"
     CDS             complement(1360..>2379)
                     /locus_tag="PKV31_09755"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNB93185.1"
                     /translation="KEGDVIQHSMITKSIERAQKRVEENNFGIRKRLLEYDDVMNIQR
                     EAIYKKRNNALFGDRLSVDLSAMFSSATEDVVLRGRQLHSFEEFRELSIRELGFDPAD
                     LGEGFTAMNEDQAIESFQVAFMKYYNTKNQRISEVILPIIKNIKEREGDRFKRIALPF
                     SDGSAHPLPVSAGMNEAIESDGRSIASDLEKTITLSIIDDKWKEHLRNMDDLKDAVQA
                     ASFEQKDPLVIYKMEAFTLFENLVGKINHEVTAYLAKGNMAIAMEPEQVREARVQKTD
                     FSKARMHKDNSEGYDDDPMREGPPKSTVEKPVTYIRSEKKVGRNDPCPCGSGKKYKAC
                     HGSHM"
BASE COUNT          602 a          537 c          478 g          762 t
ORIGIN      
        1 atcaattatt ttgaggaagg gtggttccat atcatgagtc ttgatgcact cgatcatcaa
       61 ttattcatca tttcgttggc agcacttttc ctattgaagg attggcgtcg tgttctggta
      121 ctcgtgacag catttaccat tggtcattcc cttacattag ctttaagtgc ttatgatatt
      181 attcgcttca gtactgcatg ggtcgaattc cttataccga ttaccatagt cattactgct
      241 ctttacgacc taacctatac aaatctttcg aagatagaaa aaaggaatat caatttcaat
      301 tatattatcg cactttcatt tgggctgatc catgggatgg gttttgccaa cagtatccgt
      361 atgatgcttg ccagtgacca gcgaatcggg atgccattac taagttttaa tttagggctt
      421 gaggccgggc agatagtact tatactcctt atacttttag cagctcagtt gattgtagtc
      481 aacatgaaaa ttccacggaa atactggata attctgctga gtgtaattcc actggtacta
      541 tccttcaaaa tggccttgga aagaattccc taaatagcag gtaaatcgtc ggcggaagga
      601 ttaacgcttc tttttagtgg aagttttttt cttagaatat tttgatttgg aagatgaatt
      661 tttcttagct ttattgcccg ccttactatt gcttttgggc ttgactttac ttttggcttt
      721 gggcatctca ggttttacag gctttaaagc cagggaatcc cgacttacaa aagcctgggg
      781 atttgctctg cgtgtcaatt cattttgcca gtctttggca ttgaattcaa tacaaaactc
      841 gatccacacc ggaacttttc ttgtgccttg tcgtcctgat gtccattttt caccggactg
      901 caacatcaat ttggtgagac gattagcttc attgccacat tccgacgaca aatcctgtgc
      961 aggcttaatc tccttgatct gcccagtagt gtcaaccagt gccctaaagg aatatctttc
     1021 ttccctacct gattccaaag cagtagccgg atattgcata ttagccaaca tccatgcttt
     1081 aagagatgcc accgtacagc gatccaactt atacatattg ccataacctg aggcacagga
     1141 aaccagttga ggcatatgat ccacttgagt gtatatcctc atggaatcac tttgttggct
     1201 gaaacaaaga aaggcttcca acatccaaat ggcggccaga aaaaaccttt gtcttcggaa
     1261 agtatgacgc tgctttgaaa tcaatacaaa tagttttgtc aaaaaacatc tcaacggatt
     1321 atcgttgaga tgttgcaatt atactgattt tcggataatt tacatatggg atccgtgaca
     1381 agctttatac tttttcccac ttccgcaagg gcaaggatca ttacgtccga cttttttctc
     1441 tgacctgata taggtcacgg gtttctcgac agtggatttt gggggccctt ctctcatagg
     1501 atcgtcatca taaccttcag aattatcttt gtgcatcctt gcttttgaaa aatccgtttt
     1561 ttgcacccga gcttccctta cctgttccgg ttccatagct atggccatat tacctttggc
     1621 aagataagct gtcacctcgt gattgatctt acccaccaaa ttttcaaaca aggtaaatgc
     1681 ttccatctta taaatcacca gtgggtcttt ctgctcaaac gaagcagcct ggacggcatc
     1741 cttcaggtcg tccatattac gcagatgttc cttccattta tcatcgatga tggacagggt
     1801 tatggttttt tctaaatcac tagcaatact tcttccatca gattcaatgg cttcattcat
     1861 tccggctgaa accggcaggg gatgtgcact tccgtcggag aatgggaggg caatccgctt
     1921 gaaacgatcc ccttcgcgtt ctttgatgtt cttgatgatg ggcaggatga cttcactgat
     1981 cctctggttc ttggtgttat aatacttcat aaaggcaacc tggaaacttt caatcgcctg
     2041 gtcttcattc atggcagtaa aaccttcacc caggtctgca gggtcaaagc ccagctcgcg
     2101 gatggatagt tccctgaact cctcaaagga atgcaactgc cggccacgga gtactacgtc
     2161 ctcagtggca gaggaaaaca tggcactgag gtctacagaa agcctatctc cgaagagggc
     2221 attatttctt ttcttataaa tagcctctcg ctggatattc atgacgtcat catattccag
     2281 gagtcgttta cggatgccaa agttgttttc ctctactctt ttctgggctc tttcaatgga
     2341 tttggtgatc atgctgtgtt gaatcacatc gccttcctt
//
LOCUS       DAOQMC010000311         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_28171_length_2377_cov_5.251938, whole genome shotgun sequence.
ACCESSION   DAOQMC010000311 DAOQMC010000000
VERSION     DAOQMC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28171_length_2377_cov_5.251938"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKV31_09760"
     CDS             <1..352
                     /locus_tag="PKV31_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93186.1"
                     /translation="ATVGVIYMLKLSHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQ
                     RFGEMEVWALEAYGASNILQELLTIKSDDIQGRAKAYEAIVKGDNLPEPNIPESFNVL
                     VHELRGLALDVGFD"
     gene            418..>2377
                     /locus_tag="PKV31_09765"
     CDS             418..>2377
                     /locus_tag="PKV31_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNB93187.1"
                     /translation="MSLKKKNSTPKQNFSSITIRLASPDEILERSYGEVLKPETINYR
                     SYKPERDGLFCERIFGPVKDYECYCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHI
                     KLVVPIVHIWFFRSLPNKIGYLLGVSSKKLESIIYYERYVVVQPGVKESSGVNRLDLL
                     AEEEYFEILDSLPKENQLLDDTHPDKFIAKMGAEAVRDLLMRIDLDQLSYELRHLAST
                     ETSQQRKSEALKRLRVVESFREGMETTENRPDWAVIQYLPVIPPELRPLVPLDGGRFA
                     SSDLNDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQESIDSLFDNSRKSNAVKAE
                     GGRALKSLSDILKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLKLHECGIPKGMASELF
                     KPFVIRKLIERGIVKTVKSAKKLVERKEPVIWEILENILKGHPVLLNRAPTLHRLSIQ
                     AFQPKLIEGKAIQLHPLVCSAFNADFDGDQMAVHVPLSQASIIEAQLLMLSSHNMLNP
                     QDGSPVTLPSQDMVLGLYYLTKPKVSTPESPVRGEGMKFYSSEEVLIALNEGKLDLHA
                     QIKVRERVEENDSMVLKLIDTTVGRVLFNKAVPANVPFVNELLTKKNLKKVINDIIKR
                     TNFAVTADFLDGIKEMGYFWAFKGGLSFN"
BASE COUNT          718 a          452 c          566 g          641 t
ORIGIN      
        1 agctacagta ggtgtcatct atatgcttaa gctatcgcac atggtcgatg acaagatgca
       61 cgcaagaagt atcgggccat actctctcat cacacaacaa ccactcggag gtaaggctca
      121 gtttggtggg cagcgatttg gagagatgga ggtatgggca cttgaagcat atggcgcttc
      181 taatatctta caggaattat tgaccataaa gtcggatgat atccagggaa gggctaaggc
      241 ttatgaagcc attgtaaaag gtgataacct gccggagcct aatatacctg agtcattcaa
      301 tgtattggta catgaattga gaggattggc tcttgatgtt ggatttgact gatgaattga
      361 atcgttaaat ccccatagac agccattttt aagaatccga taatttttat ataaattatg
      421 tctttaaaga aaaaaaattc aacaccaaag caaaatttct cttccattac cattcggttg
      481 gcaagcccgg atgagatttt agagcggtca tatggcgagg ttctaaaacc agagacaatc
      541 aattaccgct catataagcc tgaaagagac ggcctttttt gtgaaagaat ctttggtccg
      601 gtaaaggatt atgaatgcta ttgtggcaaa tacaaaagaa tcagatataa gggcatcgtc
      661 tgcgatcgct gcggtgtcga ggtgactgaa aagaaagtaa gacgtgagcg tatgggtcac
      721 atcaagcttg ttgtgcctat tgttcatatc tggttcttca gatctttacc caacaaaata
      781 ggttatctgt tgggagtatc ttccaagaaa ctggagtcca tcatttatta tgaaagatat
      841 gtagtggtac aacccggtgt aaaggagagc agtggtgtaa atcgattgga tctccttgca
      901 gaagaggaat acttcgaaat acttgattcc cttccaaagg aaaatcaatt gctggatgat
      961 acccatcctg ataagtttat cgccaagatg ggtgcagaag cagtgaggga ccttttgatg
     1021 agaatagatc tggaccaact atcttatgaa ctgcgacacc tggccagcac cgaaacctca
     1081 cagcaaagaa agtcagaagc cttaaagaga ctccgtgttg tagagtcctt cagggaagga
     1141 atggagacaa ctgaaaacag accggattgg gcagtaattc agtatcttcc ggttattcca
     1201 ccagagttaa ggccattggt tccattggat ggaggtcgtt ttgccagttc agatttgaac
     1261 gatttgtaca gaagagttat cattagaaac aacaggttga agcgccttct ggagatcaag
     1321 gctcccgaag tcatccttcg gaatgagaag aggatgcttc aggaatccat tgatagcctg
     1381 tttgataatt ccaggaagtc caatgctgtt aaggccgaag gaggcagagc cctgaagtca
     1441 ctttctgata tcctgaaagg taagcaggga cgattccgtc aaaacttatt aggtaaaagg
     1501 gtggattatt ccggccgttc cgtgatcgtg gtaggcccaa ctctcaagct gcatgagtgc
     1561 ggtattccta aaggaatggc ttctgagctg ttcaaaccgt ttgtgatcag aaaattgatt
     1621 gagcggggaa tcgttaagac tgtaaagtct gctaaaaaat tggtcgaaag aaaagaacct
     1681 gttatatggg aaattcttga gaatatattg aaaggtcatc cggtattgct caaccgtgca
     1741 ccgacactcc accgattgtc catacaggct ttccagccta aattgattga aggaaaagcg
     1801 atccaattgc atcctttggt atgtagtgca tttaatgccg acttcgacgg cgaccagatg
     1861 gctgtacacg taccattgag ccaggcatcc atcattgaag ctcaattgct gatgctgtct
     1921 tcacacaaca tgctcaatcc acaggatggt tcgccggtta ccttaccctc tcaggacatg
     1981 gtacttggac tttattattt gactaaacca aaggtatcta ctcctgaatc tcctgtaaga
     2041 ggtgaaggca tgaagttcta ctcttcagaa gaagtactta tcgcactgaa tgaagggaaa
     2101 ctggacctgc atgctcagat aaaggttagg gaaagagttg aagaaaatga cagcatggta
     2161 ctcaaattga tcgacaccac cgttggaaga gtgttgttta ataaggctgt tcctgctaat
     2221 gtaccatttg tcaatgagtt gttgaccaag aaaaacctta agaaagttat taatgatatc
     2281 atcaagagga caaactttgc tgttacggct gacttcttgg atggcatcaa ggaaatgggt
     2341 tatttctggg cgttcaaggg tggattatca tttaacc
//
LOCUS       DAOQMC010000312         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_28227_length_2374_cov_4.953428, whole genome shotgun sequence.
ACCESSION   DAOQMC010000312 DAOQMC010000000
VERSION     DAOQMC010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28227_length_2374_cov_4.953428"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..393)
                     /locus_tag="PKV31_09770"
     CDS             complement(232..393)
                     /locus_tag="PKV31_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93188.1"
                     /translation="MADRFAKSGLDKDVFEIEAGRKGSTLRYYLQRVKKTRSVHWHRA
                     GRIIYLRES"
     gene            680..2017
                     /locus_tag="PKV31_09775"
     CDS             680..2017
                     /locus_tag="PKV31_09775"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNB93189.1"
                     /translation="MMCRLIGIFMYLVLTGHYLNAQDYYGAEPEVVKKGLTDDKLLDE
                     IQRVTFRYFWDFADPNSGMARERSNTNTGYGHEVITSGGSGFGVMAIIVAVERGWISR
                     EEGVGRLLKMVNFLRKADSFHGMFPHWWNAETGRVRPFSRKDDGADIVESSFMFQGLI
                     CAMEYFDRDTKEERDLRGKINLTWREAEWDFFTRDGRQVLYWHWSPNNGWAMNFEIRG
                     FNECLITYVLAASSPTYAIDEKVYHNGWAMSNFFKNGKQFYGYTLPLGFDYGGPMFFA
                     HYSFMGLDPRGLKDRYADYWEQNTHHTLINRAYCIDNPKKFKGYGPDCWGLSAGDECG
                     GYSAHSPENDNGYISPTAALASMPYTPEYSMQALRYFYEKMGDKLWTRYGFLDSFNPQ
                     TGCVTPTHLAIDQGPIIVMIENHRSGLLWKLFMKNKDVQRGLTKLGFTFRSEK"
     gene            2061..>2374
                     /locus_tag="PKV31_09780"
     CDS             2061..>2374
                     /locus_tag="PKV31_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93190.1"
                     /translation="MKRLLVNIAAIFVFSMLIFSCKKEQPIELPDFVGLSATIDDISK
                     ITPTGISLRPKISIHFSSGILSTSASSAIRLEGYSGQLNVSVSDSTLLIQPEGDLAYL
                     EK"
BASE COUNT          650 a          518 c          526 g          680 t
ORIGIN      
        1 aactctgcgt tctctgcgag aaaaaaaaga aggaaagtga ttcagacaaa cgtaatcgac
       61 cgagcaacta cccatcattt ctttatatcc ccggagcagt tcatgtattc ggttgatagg
      121 ataggataga gttttattga gggtatctac gagccattca taggtgttaa ttcctttagc
      181 tttgcatgta taataaaaaa agaatacaac atagcggctc tgacggctcc atcatgactc
      241 cctcaaataa ataattcttc ctgcccggtg ccagtggacg gatcttgttt tcttgactcg
      301 ttgtagataa tatcgcagtg ttgatccttt tctccctgct tctatctcaa aaacatcctt
      361 atcaagaccg gacttcgcaa atcggtccgc cattttttca tactttgtca tggttcttat
      421 ttcccctcta aggtatgacc accgataaca tgaaaaaaga tgtacttaga cggttattta
      481 cgttctttac aggcatatta aatccgatcc ataaccatgt ccgataacca tagtacttcc
      541 agcggaatgg atttttatca atgatatggt tctatagaat tttcgactgc ctgttatcaa
      601 taaaatcaat gatgaggtaa gtatctgtac catcctcttc tttcaaaaag aaatactatc
      661 ttgcagaaat tttatcagta tgatgtgtcg tttgatcggt attttcatgt atttggtatt
      721 gacgggccat taccttaatg cgcaggacta ttatggcgca gagcctgaag ttgttaaaaa
      781 aggattgaca gatgacaagc tgctggatga gatccaaaga gtcactttcc gttacttctg
      841 ggattttgcc gatccaaact ccggcatggc gagagaacgc agcaatacca ataccggtta
      901 tggccatgag gtcatcacca gtgggggaag tgggttcggt gtgatggcca ttattgttgc
      961 tgttgaacga ggctggatca gcagagagga gggagttggc cggctattaa agatggttaa
     1021 ttttctcaga aaggcagata gcttccatgg aatgtttcct cattggtgga atgcagaaac
     1081 cggtcgtgta aggccttttt ccagaaaaga tgatggtgcg gatatcgtcg aatcctcctt
     1141 catgtttcag ggacttatct gcgccatgga atatttcgac cgggatacta aggaagagcg
     1201 tgatctccgt gggaagatca atctgacctg gcgcgaggca gaatgggact tcttcacccg
     1261 cgacggcaga caggtattgt actggcactg gagtcccaac aacggctggg cgatgaactt
     1321 cgagatacgt ggcttcaatg aatgcctgat tacctatgtc cttgccgcct cttcgcccac
     1381 ctatgccatc gatgaaaagg tgtatcacaa cggatgggcg atgagtaatt tctttaagaa
     1441 tggcaagcag ttctacggtt ataccctgcc cctcggattt gattatggag gacccatgtt
     1501 ttttgctcat tattccttta tgggacttga cccccggggg ttgaaggaca ggtacgcaga
     1561 ttactgggaa caaaatactc atcacaccct gatcaaccgt gcatactgca tcgacaatcc
     1621 gaagaaattc aagggatatg gaccggattg ctgggggttg tcagccggag acgaatgtgg
     1681 cggttattct gcacactcac cggaaaatga caatggctat atctctccga cagcggccct
     1741 tgcctccatg ccttatacac ctgaatattc tatgcaggca ttgcgatatt tttacgagaa
     1801 aatgggcgat aagctctgga cccgatatgg atttctcgac tccttcaatc ctcagaccgg
     1861 ctgcgtcaca cctacccatc tggccataga tcagggacct atcattgtaa tgatcgaaaa
     1921 tcaccgttcc ggattattgt ggaagctttt tatgaaaaat aaagatgtac agcgcggctt
     1981 gaccaaactt ggatttactt tcagatcaga aaaataactg ttttcaagat taaaacaatg
     2041 tcgattcaat ttacgatgcc atgaaacgac tgcttgttaa tattgcagca atatttgtct
     2101 tctcgatgtt gatattttcc tgtaaaaagg aacaaccaat cgaactgcct gactttgtcg
     2161 gcctttcagc caccattgat gacatatcca aaattactcc cacaggtatt tccctcagac
     2221 caaagatcag catacatttc tcttccggga tactgagcac ttcagcatca tcggcaatcc
     2281 ggctggaagg atattcagga caattgaatg tttcggtgtc cgattccaca ctgttgattc
     2341 agcctgaagg ggatcttgct tatcttgaaa agta
//
LOCUS       DAOQMC010000313         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_28957_length_2343_cov_5.242570, whole genome shotgun sequence.
ACCESSION   DAOQMC010000313 DAOQMC010000000
VERSION     DAOQMC010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28957_length_2343_cov_5.242570"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKV31_09785"
     CDS             complement(<1..295)
                     /locus_tag="PKV31_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93191.1"
                     /translation="MNWDQSEKKSREASSNQINESSEAASAFSRPAVPFQLKASEEFI
                     HENREKESTGSEYFPLNSSENGPKSFPAPPIQLKSEVVQRKADDEEGEAGMSHS"
     gene            complement(433..1938)
                     /gene="putP"
                     /locus_tag="PKV31_09790"
     CDS             complement(433..1938)
                     /gene="putP"
                     /locus_tag="PKV31_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HNB93192.1"
                     /translation="MHIYEAISLGLYMLLMLAIGVYSYRKSTSNSEEFLIGGRRLGAA
                     VTALSAGAADMSGWLLMGLPGAMYFFGVSSAWIAIGLTIGAYLNYVVVAPRLRVYTEV
                     AQNSITLPVFFENRFKDKSHLLKISSSIFILIFFTLYTSAGMVSGGRLFESAFGMDYN
                     TGVWITSLVVVIYTFLGGFLAVSLTDFVQGAIMIIALILVPLVALDKIGGVQETVTLI
                     NSKGVQYLDLFKGTTLVSIISLMAWGLGYCGQPHILVRFMAIDKPAQLAKARRIGITW
                     MIFTVGGALCVGLIGIAYLMKFDTATMEVFDKSKNEAETIFIYFTRVLFHPLIGGFLI
                     SAILAAVMSTISSQLLVTSSSLTEDIYRSLVKKDASPGHFLIAGRLSVLIVAGVALLL
                     SLNPKDSILNLVGNAWAGFGSAFGPLIVLSLLSRKTTWQGALAGMLTGGITVLLWVYL
                     DHPYKDWYEMIPGFLSSLVCNLIVSQLTYKKNPVIEAEFEQVEKMINDNQR"
     gene            2158..>2343
                     /locus_tag="PKV31_09795"
     CDS             2158..>2343
                     /locus_tag="PKV31_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93193.1"
                     /translation="MEDDLGTEVRFALVCIAKLSVVLWQTIGHHFSTFAFNAGLFSLT
                     LKCRFAGLTKMYQWCRVI"
BASE COUNT          668 a          516 c          514 g          645 t
ORIGIN      
        1 aactatggct catgcccgcc tcgccttctt catcatcagc ctttcgttgt accacttcac
       61 tctttaactg aatgggtggg gcgggaaatg attttggtcc attttcggat gaattcaatg
      121 gaaaatattc agaacctgta gattccttct ctcgattttc atggataaat tcttcggagg
      181 cttttaattg aaagggaaca gctggccgac tgaaagcact ggcagcctcc gatgattcat
      241 tgatttgatt ggaggaagcc tcccgagatt ttttctcaga ttgatcccag ttcatagatg
      301 gagattttat ttaatgtgca tgtaaaatta ataaaacaat gctttacttt cttttaattg
      361 gtctgagtat tttattaaag taagacaatt atataaataa atacacccga atacggcagt
      421 ataatattgt ttctagcgtt ggttgtcatt aatcattttc tctacctgct caaactccgc
      481 ctcaattacc ggattcttct tgtacgtcag ttgtgaaacg atgagattac agactaatga
      541 agacaggaaa cccggaatca tttcatacca atccttgtaa ggatggtcga gatataccca
      601 gagtaaaaca gttatgcctc ctgttaacat gccggccaac gctccttgcc aggtcgtctt
      661 tctgctcaac aaggacaata caatcagagg tccgaaggct gaaccgaagc cggcccaagc
      721 atttcctacc aggttgagga tgctgtcctt ggggtttaag gaaagtaaca gggctactcc
      781 ggctacaatc agcacggaca gtcttccggc gatgagaaaa tggcccggac ttgcatcctt
      841 ttttaccaga gagcggtaaa tatcctcagt cagcgaactg gatgtgacca gcaattgtga
      901 ggagatggta ctcattactg ctgccagtat ggctgaaatc agaaaaccac cgatcaatgg
      961 gtgaaacaac acacgtgtga aatatataaa tatggtttcg gcctcattct ttgacttatc
     1021 gaaaacctcc atagtagctg tgtcgaattt catcaggtat gcgataccga tcaggcctac
     1081 gcacaaggcc ccgccaaccg tgaatatcat ccaggtaata cctatacgtc gggcttttgc
     1141 caattgagcc ggcttatcta tggccataaa gcggacgagg atgtgtggct gaccgcaata
     1201 cccgagcccc caggccatca gggaaattat acttacgagg gttgtaccct tgaaaagatc
     1261 aagatactga acacccttgc tgttgatcag tgtcaccgtt tcctgtacgc caccgatctt
     1321 atccaaagcg accagaggaa caaggatgag ggcgatgatc atgatggcac cctggacgaa
     1381 atctgtcaga ctcacagcca gaaatccccc caaaaaggta taaatcacga ctacaaggct
     1441 ggtgatccat acaccggtat tataatccat accaaatgcg gattcaaaga gcctccctcc
     1501 ggataccatg ccggccgagg tataaagggt aaagaaaatc aggatgaaga tggacgatga
     1561 aatcttcagc aggtgacttt tgtccttgaa tctattctcg aaaaagacgg gaagtgtgat
     1621 ggagttttgg gccacctccg tatatacacg cagtcgtggt gccactacta cataattgag
     1681 atacgcccct atcgtaagtc cgatggcaat ccaggcactg gagacgccga aaaagtacat
     1741 ggcaccgggt aggcccatca aaagccagcc actcatgtca gcggcaccgg ccgacagcgc
     1801 agttaccgcc gcacccagcc ttcgccctcc aatgagaaat tcttctgagt ttgaggttga
     1861 cttgcgatag gaatacacac caatcgccag catcaacaac atatataatc ctaaggaaat
     1921 tgcttcataa atatgcataa tatgattctt tttggatagg agaggatctc cttattatcc
     1981 gatcaagcat cgaatttaaa tgaaattggc agtagtatat agttttttcg gtttttttta
     2041 tcggttgatt caaaagtatg tcaatttgaa cacaatgcca agggattgga tgagtcaaag
     2101 gtaagtgtgc atccggcgtg tattttattt ccttgtcggt ttctgaaact attattattg
     2161 gaagatgatt tgggaacaga agtcagattt gcgttggttt gtatcgcaaa gttatcagtt
     2221 gttttatggc aaacaattgg tcatcacttt tcaacctttg catttaatgc cggcttattt
     2281 tctttgactt tgaagtgccg atttgccgga ctaactaaaa tgtaccaatg gtgcagagtg
     2341 att
//
LOCUS       DAOQMC010000314         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_29440_length_2322_cov_4.214821, whole genome shotgun sequence.
ACCESSION   DAOQMC010000314 DAOQMC010000000
VERSION     DAOQMC010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29440_length_2322_cov_4.214821"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2202
                     /gene="secD"
                     /locus_tag="PKV31_09800"
     CDS             <1..2202
                     /gene="secD"
                     /locus_tag="PKV31_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNB93194.1"
                     /translation="IWDKKTDSLGVVIDSTMRIVDVPVSNTMADAGPLFKIFTPNSAT
                     QQGIAFPLSVMGVADKNKKKLIDEYLNMPQVKALFPADISFKWASKPTKDPETFKYTN
                     KYELYGIKVPRSGKAPLEGDRVVDARETQDQMSNQVAVSLRMDNEGAKKWGEMTTKAA
                     ADQNREIAIVLDGEVVSAPRVNNAITSGDSQITGDFSVQEGKDLANILQIGKLPAGTR
                     IVQETLVGPSLGQENINKSIIAILIGLLFIIIFMISYFATAGAIAVITLLCNMFFIFG
                     VLASKGTVLTLPGIAGILLTMGIAVDVSVIIFEWVKEELKHGYGLKEAISRGFKHSYS
                     AIIDANIITLITNIILAYFGLGPIKGFAVVMIIGVLSSLFTAVLVGRMITDWWANKGK
                     EIKFSTSFSDKFFHGANVNWMGLRKFAYIFSSSMVLIGLISIFTRGFELGVDMKGGYS
                     ANVSFVEGTKVDAETFRDYLKKELGGEPIVKAVDVKNTYNITTSYKIDDPNPDVNEEV
                     LAKIHKSVVEITKKDIPLDEFKKADAKGTHIASFSKVGPTVADDIKTSSFTAAILALA
                     AIFIYILFRFNRWQFSLGAIIALVHDSLVVLGLFSLLHGFVWFSMEIDQAFIAALLTV
                     IGYSMNDTVIIFDRIREFAGTNLSSTKTEVINAAINSTLGRTIITSLITFGVMIIMFI
                     FGGDSIKGFTFAILIGIVFGTFSSIFIAGSIVHDLVADIRPKTVAGKAATK"
BASE COUNT          668 a          443 c          528 g          683 t
ORIGIN      
        1 atttgggaca aaaagacgga tagcctaggt gtagtaattg attccaccat gaggattgtt
       61 gatgtgcctg tatctaatac aatggctgat gcaggtccac tgttcaaaat atttactccc
      121 aactcagcta cacagcaggg aatcgcattc cctctgtctg taatgggtgt tgccgataag
      181 aataaaaaga aattgatcga tgaatatctg aatatgccac aagtgaaggc attgttccct
      241 gcagatattt ccttcaagtg ggcatccaag ccgacgaagg atcctgaaac cttcaaatat
      301 accaacaagt acgagttgta tggtattaag gtgccacgtt ccggtaaagc gccattggaa
      361 ggagaccggg ttgtagatgc cagggaaaca caagatcaaa tgtcaaatca ggtagctgta
      421 tctctccgaa tggacaatga aggtgccaag aaatggggtg agatgacgac caaggcagct
      481 gccgaccaaa acagggaaat cgctatcgtc cttgatggag aagtcgtctc agcaccaagg
      541 gtaaataatg ccatcacttc gggtgattcg cagattacag gagactttag tgtacaggaa
      601 ggtaaagacc ttgccaatat ccttcaaata ggtaagttac ctgccggaac aaggattgta
      661 caggagaccc tggtcggacc ttcactgggt caggaaaata tcaacaaatc cataatcgcc
      721 atcttgatag gattattgtt tatcatcatc ttcatgatca gctattttgc cacggcaggt
      781 gcaatcgcag taataaccct actctgtaat atgttcttca tatttggggt acttgcctcg
      841 aaaggtaccg tacttacctt gccgggtatt gctggtatcc tgcttactat gggtattgct
      901 gtcgatgtaa gtgtaattat ctttgaatgg gttaaggaag agcttaaaca tggatatgga
      961 ttgaaagagg ctatttcacg tggtttcaaa cattcttatt ctgcaattat cgatgccaac
     1021 atcatcacat tgattaccaa cattatactt gcttacttcg gtttaggtcc aataaaaggc
     1081 ttcgcagtag taatgatcat cggtgtattg tcttccttgt tcactgctgt tttggtaggt
     1141 cgaatgatta cagactggtg ggctaataaa ggtaaagaaa tcaagttctc tacttcattc
     1201 tctgataaat ttttccacgg agccaatgtc aattggatgg ggctgcgcaa atttgcttac
     1261 atcttctctt cttctatggt tctgattgga ttaatctcca tcttcacacg agggttcgaa
     1321 cttggtgtag atatgaaggg aggatactca gccaacgttt cctttgtgga aggcactaag
     1381 gtagatgctg aaacgtttag agattatctg aagaaggaat tgggaggaga acctattgtt
     1441 aaggctgtgg acgtcaagaa tacatacaac atcaccacat cctacaagat cgatgatcct
     1501 aatccggatg tcaatgaaga ggtattggcc aagatccata agtcagtggt agagataacc
     1561 aaaaaagata ttcctctgga tgaattcaaa aaagccgatg cgaaaggtac gcacattgcg
     1621 agtttctcga aagttggacc taccgtagcg gatgacatta aaacaagttc atttaccgcc
     1681 gctattttag cccttgcagc gatattcatc tatatcctgt tccggttcaa cagatggcag
     1741 tttagcttgg gtgcaatcat cgccttggtt cacgacagtt tggtcgtatt ggggctcttc
     1801 tcactattgc acggttttgt ttggttttcc atggaaatag atcaggcatt catagctgcc
     1861 ttgttgacag tgattggtta ttcgatgaat gatacggtga tcatttttga caggatcagg
     1921 gaattcgcag gcacgaatct gagttcgacc aagactgagg tgataaatgc ggctatcaac
     1981 agtacattag gccgtaccat tattacatca ttgattacct ttggtgtgat gataatcatg
     2041 tttatctttg gtggagacag tatcaaaggc tttacatttg ccattttaat tggtattgta
     2101 ttcggtacgt tctcaagtat attcattgcc ggctcgattg ttcacgacct tgtcgcggat
     2161 atcaggccga aaacagtggc gggcaaggct gccactaaat aaatacaacc gtttttattt
     2221 gagaaataga aaggctgcaa ttggtataat tgcagccttt tttatatttt tggccaattt
     2281 aatttgaaac ggataaaaaa ttttaataca tgaatttcga ag
//
LOCUS       DAOQMC010000315         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_29498_length_2320_cov_2.937748, whole genome shotgun sequence.
ACCESSION   DAOQMC010000315 DAOQMC010000000
VERSION     DAOQMC010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29498_length_2320_cov_2.937748"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKV31_09805"
     CDS             <1..285
                     /locus_tag="PKV31_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93195.1"
                     /translation="NPTGLNNTDSVNNQFINQAIIFNTFPVSGITDNNFKYKSAFNPI
                     DKGPYVGIKPYTFGLVPAIPSIYELTVPATFVNGGSGNTLQITISTRGNQ"
     gene            567..>2320
                     /locus_tag="PKV31_09810"
     CDS             567..>2320
                     /locus_tag="PKV31_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNB93196.1"
                     /translation="MPHIVAMEYFFDTDPGFGNGIQIPVATDTAIHGLNVNIDLTNIG
                     DGIHTLFIRSRDNYGHWSLTNRREIAQIPRITAMEYFFDIDPGFGNGIPILISADTAI
                     ANLNVLLNVGFLTSGMHTLFIRSKDGYGKWSLTNRRNFNYCPSGMTYFYQDLDLDGYG
                     TLADSVLDCVAPAGYVANFEDCDDGNPGIHQKFPFYSDSDSDGFGSGNPILICAQDML
                     SAPTGFATNNSDCNDNDPNIYAYFPFFTDADGDFFVASLDTFYFCSSVYSAPNTPPPG
                     YISTSLSLGLDCDDNQYGFVSSFPFYIDSDHDGYGTGTQVQVCANSATLPPPGYSILA
                     GDCNDNNALIHPGAMEICDGLDNNCDGLTDTITVTHFVCGIATSEGQSFTLTAPPGKV
                     FTAVDFASYGTPNGTCGNFTLGVCHASSTMPIVQALVIGQNSVTLTQNYQIFGDPCVG
                     VYKRYYVQASYTDTISLKYPFFADADGDGYGAGSPVFLCATNANTAPAGYSLNGDDCD
                     DTKSSVYPGVTDRMEYFIDNDPGPGNGTPITLSALLNTDTINQSLSISLPTNLSPGHH
                     VLATRVGGCSVGWGLLEHR"
BASE COUNT          650 a          497 c          481 g          692 t
ORIGIN      
        1 aatccgacag gcttgaataa caccgacagt gtgaacaatc aatttataaa tcaggccatt
       61 atcttcaaca cattcccggt ttcgggtata actgataaca attttaaata caaatctgcc
      121 tttaatccaa tcgacaaagg gccttatgtt gggattaaac catatacatt tggtttggtt
      181 cccgccattc catccatata cgaattgaca gtacctgcaa cctttgtgaa tgggggatca
      241 ggtaataccc ttcaaattac catcagcaca agaggaaatc aataaccagt aaaaaatgac
      301 tgatgaaaaa tcgtatttta atttggataa tgctttgcgg attaaaggtt acatctcaga
      361 atataatccg catagaatat ttttttgata atgatccggg tttcggtaat ggaatatcga
      421 ttcccattgt aacaggttca actattgcaa atttaaatgc gaccatcagt ttgtcgtcaa
      481 tcagtgaggg aaaacatatg ctttatgttc gctcacagga tgatcaggga aattggagca
      541 ttacaaacaa aaggcaaatt gctcagatgc cacacattgt tgccatggag tatttttttg
      601 ataccgatcc cggattcgga aatgggattc aaattcctgt ggcaacagat actgccatcc
      661 atggattgaa tgtgaatatt gacttaacaa acattggaga tggtattcac acccttttta
      721 tacgttcgag agacaactac ggccattgga gccttacaaa ccgacgggaa attgcacaaa
      781 ttccccgtat tacagccatg gaatattttt ttgatattga tccgggattc ggcaatggaa
      841 ttccgatttt aatatcagcc gacactgcaa ttgctaattt aaatgttcta ttaaatgtgg
      901 gtttcttaac ttctggtatg catactctgt ttattcgatc taaagatgga tacggtaagt
      961 ggagtttaac taatcgccgc aattttaatt attgtccttc cggtatgact tatttctatc
     1021 aggatcttga tctggatgga tatggaactt tggcagatag cgtactagat tgtgtagccc
     1081 cggcaggcta tgtagccaat ttcgaagatt gcgatgatgg aaatccaggt atccaccaga
     1141 aatttccgtt ttattcagat tccgacagcg atggcttcgg aagtggtaat ccaatactga
     1201 tttgcgcaca ggatatgttg agtgccccca caggatttgc tacaaataat tcagattgta
     1261 acgataatga ccccaacatc tatgcgtatt ttcccttttt caccgacgca gacggagatt
     1321 tttttgtggc ttccttagat actttttact tctgctcaag tgtctattct gcgcccaata
     1381 ccccaccgcc aggatatatt tctacctcgc tatcacttgg tttagattgt gatgacaatc
     1441 aatatggttt cgtttccagt tttccttttt atatcgattc agaccatgat ggctacggca
     1501 caggaactca ggtacaggtt tgtgccaata gcgcaactct tccaccgcca ggttattcca
     1561 tccttgccgg tgattgcaac gacaacaacg cactcattca cccgggcgcc atggaaattt
     1621 gtgatgggct tgataataat tgtgatggct taaccgatac tataactgtt acccattttg
     1681 tatgcggcat cgcaacatca gaaggacaat cgtttacttt aaccgcgcca ccaggaaaag
     1741 tatttacagc tgttgatttt gcaagctatg gaacacccaa tggcacttgt ggcaatttta
     1801 cattgggtgt ttgtcatgct tcaagtacta tgcctattgt gcaggcattg gtgatcgggc
     1861 aaaacagcgt caccctcacg cagaactatc aaatttttgg agacccatgc gtgggtgtgt
     1921 ataaacgata ttatgttcag gcgagttata ctgataccat ttcactaaaa tatcccttct
     1981 ttgcggatgc agatggcgat ggttacggag ccggtagccc ggtatttctt tgtgccacga
     2041 atgccaatac agcaccagca ggttattcat tgaatggcga cgactgcgac gatacgaaaa
     2101 gttccgttta cccgggtgtc acagacagaa tggaatactt catcgacaat gatcccggtc
     2161 ccggcaatgg taccccgata accttatcgg ccttattgaa taccgatacc atcaatcaat
     2221 cgctatcaat cagtctgccc acaaatttat ctccgggcca tcatgtgctg gcaacgcgtg
     2281 ttggcggatg ctcagttgga tgggggctgc ttgaacatcg
//
LOCUS       DAOQMC010000316         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_29535_length_2317_cov_4.804156, whole genome shotgun sequence.
ACCESSION   DAOQMC010000316 DAOQMC010000000
VERSION     DAOQMC010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29535_length_2317_cov_4.804156"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="PKV31_09815"
     CDS             complement(<1..1609)
                     /locus_tag="PKV31_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNB93197.1"
                     /translation="MEITEPIESKNFLQEIIENDIAEGKHDGRVLTRFPPEPNGYLHI
                     GHAKSICINFGLAKQYNGQTNLRFDDTNPVTEDTEYVESIKEDIKWLGFDWANEYYAS
                     DYFDTLYDYATQLIGKGLAYVDDLTAEQIAELKGTPTEPGKNSPHRDRSVEENLDLFR
                     RMKDGEFPEGSKVLRAKIDMASPNMHMRDPLLYRIKYAHHHRTGDKWCIYPMYDFAHG
                     QSDSIETITHSICTLEFIPHRPLYDWCIEKLEIFPSRQYEFARLNMNYTVMSKRKLLQ
                     LVNENHVSGWDDPRMPTLSGMRRRGYPPKSIVEFCDKIGVAKRENIIDIGLLEFCVRE
                     DLNKTATRVMVVMDPVKLVITNYEGTGEHLSAENNPEDQSAGHRNISFGKVIYIERED
                     FMLDPPKGYFRLSPGNKVRLKYAYIIEYENHITDSEGRITEIHVKYYPDSKSGEDTSS
                     IKVKTALHWVPESAINAEIRLYDRLFVVENPNSDKDTDFKEFINKDSMVVNTRAMLEP
                     ILKDADFGKSYQFFRLGFECEASAHCVN"
     gene            complement(1621..>2317)
                     /gene="argS"
                     /locus_tag="PKV31_09820"
     CDS             complement(1621..>2317)
                     /gene="argS"
                     /locus_tag="PKV31_09820"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNB93198.1"
                     /translation="KVLFEICKALKAPYADGLHHLSYGMVELPSGRMKSREGTVVDAD
                     DLIAEVINEATKNASERNELIDLPQEEQNEINRKVGMAALKYHIIKVNPKKKMIFDPK
                     ESVDLQGHTGPYIQNAFVRISSVLRKLESEVTTDFIAYNPINEDEKELLMQLSAYKGV
                     ISNAAESYDPSVLANYTYSLAKSFHKFYHNHSILKAESPDAKSFRLSLSKVVATILAD
                     AMHILGIEMPEKM"
BASE COUNT          601 a          492 c          453 g          771 t
ORIGIN      
        1 gattaacgca atgtgcggac gcttcgcact caaagccaag cctgaagaac tgatatgatt
       61 ttccgaaatc agcgtctttc aatattggtt caagcatagc ccgggtattt actaccattg
      121 agtctttgtt gataaattcc ttgaaatcag tgtctttgtc actgttcgga ttttcgacta
      181 cgaataggcg gtcgtacaat ctgatttcgg catttatggc tgactccggt acccaatgca
      241 gtgcggtttt gaccttgata cttgaagtgt cttcacctga ttttgaatca ggataatatt
      301 tgacatgaat ttcagtaatt cgaccttcac tgtctgtgat gtgattttca tactctataa
      361 tgtaggcgta tttaagcctg accttattac cgggtgataa cctaaagtat ccctttggtg
      421 ggtcaagcat aaaatcctct ctttcaatat atattacttt accaaacgag atattacggt
      481 gaccagctga ctggtcttca ggattattct ctgcagacaa atgttcgcct gtgccctcat
      541 aattggtgat aaccaacttc accggatcca ttactaccat aactcgggta gctgtcttgt
      601 tcaggtcttc tcttacacaa aattcgagaa ggccaatatc tatgatgttt tctcttttgg
      661 caacgcctat tttgtcacaa aactctacaa tactctttgg cggataacct cttctgcgca
      721 taccgctaag tgtaggcatc cgaggatcgt cccatccact gacatggttt tcattgacca
      781 attgaagaag cttacgcttg ctcatgacgg tataattcat attgagcctc gcaaattcat
      841 attgcctgct tgggaaaatc tcaagttttt cgatacacca gtcatacaat ggtctgtgtg
      901 gaataaactc taatgtacag attgaatgtg taatggtttc gatagaatca gactgaccgt
      961 gtgcgaagtc gtacatcggg tatatgcacc atttgtcgcc ggtacggtgg tggtgagcat
     1021 atttgattct gtataggagc ggatcgcgca tgtgcatatt gggcgatgcc atatcgattt
     1081 ttgcacggag taccttggat ccttcaggaa attcaccgtc cttcattcgc ctgaacagat
     1141 ctaagttttc ctctaccgat ctatctctat gcgggctgtt ttttccgggt tcagttggtg
     1201 ttccttttag ttcggctatc tgttctgcag ttaggtcatc gacataagcc aggcctttac
     1261 caatgagttg tgtagcgtag tcatataaag tatcaaaata atctgatgcg taatactcgt
     1321 tagcccaatc aaacccgagc catttgatat cttctttaat agactcaacg tattctgtat
     1381 cttcagtcac aggatttgta tcatcaaatc tcagattggt ttgtccgttg tattgttttg
     1441 ccagaccaaa attgatacat atcgacttgg catggccaat gtgcagatat ccattaggct
     1501 ctggtggaaa ccttgtcagt accctgccgt catgtttgcc ttcggcgata tcattttcga
     1561 tgatttcctg taaaaaattt tttgattcaa taggttcagt tatttccata aatacgaata
     1621 ttacatcttt tcaggcattt ctattcccag aatgtgcatt gcgtcagcaa gaatggtagc
     1681 caccacttta ctaagactga gtctgaaaga ttttgcatca ggactttctg ccttcagaat
     1741 gctgtgattg tgataaaatt tgtgaaaact cttggcaagg ctatatgtat agtttgccag
     1801 tacagacgga tcatagcttt ctgctgcatt acttataacg cccttgtaag cagatagttg
     1861 cattagcaat tccttttcat cttcattgat agggttataa gcaataaaat ctgtggtgac
     1921 ttctgactct agctttctta gaacagatga tatacgcaca aaagcatttt gtatatatgg
     1981 gccggtgtga ccctgaagat caacagattc cttagggtca aaaatcattt ttttcttagg
     2041 attgaccttg ataatatggt atttcaaagc agccatacct acttttctgt ttatttcatt
     2101 ttgttcctcc tgaggtaaat caatcaattc gttacgttca cttgcattct tagttgcttc
     2161 attaattacc tcagctatca gatcatctgc atctaccact gtaccttcac gcgacttcat
     2221 gcgaccggag gggagctcaa ccataccata tgacaggtga tgcaagccat ctgcataagg
     2281 tgcctttagt gctttacaaa tttcgaacaa aaccttg
//
LOCUS       DAOQMC010000317         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_29586_length_2315_cov_5.451327, whole genome shotgun sequence.
ACCESSION   DAOQMC010000317 DAOQMC010000000
VERSION     DAOQMC010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29586_length_2315_cov_5.451327"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKV31_09825"
     CDS             <1..280
                     /locus_tag="PKV31_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93199.1"
                     /translation="KNAVEIQKSKQISNRENELGQLNNLISGKTEQINKLQAEIEEHK
                     NMLKSLESEISNAREKIEITIADFNVTYDGLVMKIKNDVDRIKTYINN"
     gene            291..1316
                     /locus_tag="PKV31_09830"
     CDS             291..1316
                     /locus_tag="PKV31_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93200.1"
                     /translation="MDQQSFTPKSFWQRPEGKTGVVFGIGILAGLGYLLYKTLPFLIS
                     LTSNLLYLSFLLIALGAILYMVLDPKMRNLVWYMYKSIMRWITGIFVQIDPIGILKSY
                     VDTLKNNLSKMDQQISKLRGQMHKLKEVIMNNKKQITANLNLASEAKERNKESMMILK
                     SRQAGRLTDSNMKLEDLYKKMEILYRVLTKMYENSEIMMEDIKDQVSVKEQERNAIRA
                     SHSAMKSAMSIISGDKDQRAMFDQALEAIADDVSAKMGEMERFMEMSSNFMDSVDLQN
                     GVFEEEGLKMLEMWEKEGASLLLGTEKTKIINKANDDDDVLDLNAPIPHPVKEEARLN
                     QYDSFFE"
     gene            1353..>2315
                     /locus_tag="PKV31_09835"
     CDS             1353..>2315
                     /locus_tag="PKV31_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB93201.1"
                     /translation="MSTRLTPFSRLLIVVAILSGVVFGGKYLIDKGVFGGSSSTDNSN
                     TDSSIKQGKTLPIDDDVLNVQVFTFGNAAPGLYFNNGTEPNENSRFYKDFGLKVKFHV
                     IDDFDASRQAFKGDKVQLFNNETSAMATEMEGLAPFDPQVVMQLDWSRGADAVVARRG
                     INSINDLKGKKVAVTPSTPSQTLLLFMLEAANLKPADVKMVEVPTPADAETAFKSGKV
                     DAAVVWDSQASLREVPGSKELLSTTTASNIIADVFMAKKSWVDANRDKLRKFYDGWMT
                     AVAEINGNPAAKEKAAAIMASNMQLSKEDALGMISALRLTNHGDN"
BASE COUNT          741 a          442 c          529 g          603 t
ORIGIN      
        1 taaaaacgcg gtagaaatcc agaagtcaaa gcaaatatca aaccgggaaa atgagttggg
       61 gcaactgaat aacttgattt ccgggaaaac cgaacaaatc aacaaattgc aggctgaaat
      121 cgaagaacat aaaaacatgt tgaagtcact tgagtccgag atttctaatg ccagggaaaa
      181 aatcgaaata acaattgcag attttaatgt gacatatgat ggtcttgtca tgaagatcaa
      241 gaatgatgtc gataggataa aaacgtatat aaataattaa tttcaatttt atggatcaac
      301 aatcatttac gcctaaatca ttctggcaaa ggcctgaagg caagaccgga gttgttttcg
      361 gtataggtat tctggcaggt ttggggtatc ttttgtataa aacactacct tttcttattt
      421 cgctgacatc caatttattg tatctgtcat tcctgctcat tgctttggga gccattcttt
      481 atatggttct cgatcctaaa atgagaaatt tggtttggta tatgtacaaa agtatcatgc
      541 gttggattac aggtatcttt gttcagattg acccaatcgg catcctaaaa tcatatgtgg
      601 atacgctgaa gaataacctt tcgaagatgg atcaacagat atccaagctt agaggtcaga
      661 tgcacaagct taaagaagtc atcatgaaca ataagaagca gatcacggct aaccttaatc
      721 tggcaagtga agcaaaagaa cggaataagg agtccatgat gattctaaaa tcccggcaag
      781 ccggtaggtt gacagattct aatatgaaat tggaggatct ttacaaaaag atggaaatcc
      841 tctaccgtgt tcttaccaag atgtacgaaa acagtgagat tatgatggag gacatcaaag
      901 atcaggtctc agtcaaagaa caggaaagaa acgcaatccg tgccagccat agtgccatga
      961 aatccgctat gtccatcata tcaggagata aggatcagag agccatgttt gatcaggccc
     1021 tggaggctat agccgatgat gtgagtgcca aaatgggtga aatggaacga tttatggaga
     1081 tgtcttccaa cttcatggac agtgtagatc tgcaaaatgg tgtctttgag gaagaaggac
     1141 tcaaaatgct ggaaatgtgg gaaaaagagg gcgcatcatt gctgttggga accgaaaaaa
     1201 ccaagatcat taataaggcc aatgacgatg atgatgtgct ggatctgaac gctcccatcc
     1261 cacatccggt aaaagaagaa gcacgattga atcaatatga ttccttcttt gaataaagag
     1321 cttagatttt tataatttta aaaaacaaca aaatgtcaac acgattaact cccttctccc
     1381 gactcttaat tgtagtggcc atactttcag gtgtggtatt cggtggaaaa tacctgattg
     1441 ataaaggcgt tttcggtggt agtagttcca cggataattc caacacagac tcgtcaataa
     1501 aacaaggcaa aaccctgcca attgacgatg atgtcctgaa tgttcaggta tttacctttg
     1561 gcaatgctgc accaggcttg tatttcaaca acggtacaga gccaaacgag aactccagat
     1621 tttataagga ttttggcctg aaggtcaagt ttcatgtcat tgatgatttt gatgcttccc
     1681 gccaggcatt caagggtgac aaggtacaat tattcaataa tgagaccagc gctatggcaa
     1741 ctgagatgga aggtttggca ccatttgatc ctcaggtggt gatgcaactg gactggtcga
     1801 gaggtgctga cgctgttgtc gcccgcagag gtatcaacag tatcaatgac cttaaaggga
     1861 aaaaggtggc ggttactcca tctacacctt ctcagactct gttattgttt atgctggaag
     1921 ctgccaactt aaagcctgct gatgtaaaaa tggttgaagt acctacccct gctgatgctg
     1981 agacggcctt caaatccggc aaagtggatg ctgctgtggt atgggattca caggcttccc
     2041 taagggaggt tcccggttcc aaagaactgc tttccacaac aactgcatcc aatatcattg
     2101 ccgatgtctt tatggcaaag aaatcctggg tcgatgctaa cagagacaag cttcgcaagt
     2161 tttatgacgg ctggatgact gccgtagctg aaatcaacgg taatcctgca gcaaaggaaa
     2221 aggcagcagc catcatggct tccaacatgc aattgagcaa ggaggatgcc ttgggtatga
     2281 tcagtgcgct gagactaacc aaccatggag acaat
//
LOCUS       DAOQMC010000318         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_29603_length_2314_cov_5.594068, whole genome shotgun sequence.
ACCESSION   DAOQMC010000318 DAOQMC010000000
VERSION     DAOQMC010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29603_length_2314_cov_5.594068"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PKV31_09840"
     CDS             <1..1284
                     /locus_tag="PKV31_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNB93202.1"
                     /translation="APAIVLSVVMGWDLKVTIVALSILTIAYTTLGGYKAVAQTQQYQ
                     MVVIFLGLFLTFFILLYSMPKGIGLSESLALAGQSNKMQLVDFRFNPEDRYNIWSGIF
                     GGFFLFMSYFGTDQSQVGRYLSGASIRESRLGLLFNGLLKIPMQFFILLLGVLVFVFF
                     IFNKAPLHFNPVNTQIVETGLYKSQWNDLNRRYDELFHLRQSAYKQSLESNDKSTAVE
                     RYEEQLRGLRAEADSLIYLSNPALDVNDKDYVFISYVLRYLPVGMVGLLIAVIFSSSM
                     SSKASELNALASTTIVDLYRRSIRKGRSDKHYLSATKWSTVIWGILAMLFAMFTSLFE
                     NLIQAVNIVGSLFYGAVLGIFAVAFFFRKIGGNAVFWAAIAAEGIILSLYVLGEMKVL
                     SIGFLWYNLIGCVLVIAFGMVFQAIDNRKSANRKE"
     gene            complement(1391..>2314)
                     /locus_tag="PKV31_09845"
     CDS             complement(1391..>2314)
                     /locus_tag="PKV31_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNB93203.1"
                     /translation="MKCQKDSIVRQKNGFRTFDCKTYLTFVPQMLKMKFKVFSTLCVI
                     LLLLSCKREPKPISVDIPVSDVPKIDYRVTGIIPHNTSFFTEGLLIYNGKFLESTGSP
                     EELENVESIVATVDISDGKTEEKIRLDKTRYFGEGIVVLHDKLYQITYKNQKGFIYDA
                     KTFKKLGEFGYPSKEGWGMTTDGTHVIMSDGTDMLSFLLPPEMKVVKTIRVTQNDISV
                     RNVNELEWIKGYIFANVWMTNDIIKIDPSNGKVVAKMDVSSLKHEANATWKYSEVANG
                     IAYDSTSNKIYLTGKLWPSIYRIELIENNVQ"
BASE COUNT          570 a          456 c          526 g          762 t
ORIGIN      
        1 gcacctgcaa ttgtcctgag tgtggtaatg ggctgggatc tgaaggtgac tattgtagcc
       61 ttgagcattc tgaccatagc ctatacgaca ttgggtggct ataaggcggt ggctcagacg
      121 caacagtatc agatggtcgt catcttcctg ggactctttc ttactttttt tattttgctt
      181 tatagcatgc caaagggtat tggattgtct gaaagtctgg cattggcagg gcaaagtaat
      241 aaaatgcaat tggttgattt taggtttaac cccgaggata ggtacaacat ctggtcgggt
      301 atattcggag gcttcttcct ttttatgtct tattttggca ccgatcaatc acaggttgga
      361 cggtatctct caggagctag tatccgggaa agcaggctgg gattgctttt caatggactc
      421 ctgaagattc cgatgcagtt tttcatttta ctgctaggtg tgcttgtgtt tgtatttttt
      481 atttttaaca aggcgcccct gcatttcaat ccggtgaata cgcagattgt cgaaaccgga
      541 ctttataaaa gccagtggaa cgacctaaac cgacgctatg atgaattgtt tcatttgcgg
      601 caatcggctt acaagcaaag tcttgaaagc aatgacaaat caactgcggt ggaaaggtat
      661 gaagagcagt tgagaggatt gagagcagag gctgacagct tgatttatct ttctaatccg
      721 gcactggatg tcaatgacaa ggattatgtt tttatttcct atgtgcttag atatcttcct
      781 gtcggcatgg ttggacttct gattgctgtt attttctcca gcagcatgtc gagtaaggct
      841 tctgaactga atgcacttgc ctcaacgacc attgtggatc tgtatcggcg ttctatccgc
      901 aaaggtcgat ctgacaagca ttatttatca gctactaagt ggagtactgt tatctggggc
      961 attctggcca tgcttttcgc catgtttacc agtttgttcg agaatctgat tcaagcggtg
     1021 aatattgtag gctcactctt ttatggtgca gtcctcggca tatttgccgt tgccttcttt
     1081 ttcaggaaaa taggagggaa tgctgtattc tgggcggcca ttgctgctga ggggattatc
     1141 ctttcgctgt atgttctagg ggaaatgaag gtattgtcca ttggatttct ctggtataat
     1201 ctgataggat gtgttctggt tattgccttt ggaatggtct ttcaggctat agacaatcga
     1261 aaatcagcca ataggaaaga ataaaatatt gcgccggtaa aaatctatgg aggatgcccg
     1321 gctgatttat gttgcaagta agacgctcaa ttcctgcttg taagctatac tgactaaccg
     1381 gctttgaaaa ttattgaacg ttattttcta tcagttctat tctgtatata ctcggccaca
     1441 gcttgcccgt gaggtatatc ttattggacg tagaatcata ggcgatgcca ttggcgacct
     1501 cgctgtattt ccaggtcgca ttggcttcat gtttcaaact tgaaacatcc attttggcga
     1561 ctacctttcc gttggatgga tctatcttta ttatgtcgtt ggtcatccac acattggcaa
     1621 atatgtagcc tttaatccat tccagttcgt tgacattgcg tacacttatg tcattttggg
     1681 tcacgcggat ggttttaacg actttcattt caggtggaag caggaaggaa agcatatccg
     1741 tcccatcgct catgatgaca tgagtgccgt cggtggtcat tccccatcct tctttgctcg
     1801 gatagccgaa ttctcccagt tttttaaatg tttttgcatc gtagataaat cctttctggt
     1861 ttttatacgt gatctggtac aatttatcat ggagtacgac gataccttca ccgaaatacc
     1921 tggttttatc tagccttatt ttttcttctg tctttccatc tgaaatatca acggtcgcca
     1981 caatagattc gacattttcc aattcttccg ggctgcctgt gctttcgaga aatttaccat
     2041 tataaatcag caagccttcg gtaaaaaatg atgtgttgtg tggtatgatt ccggttactc
     2101 tgtaatcgat ttttggtaca tctgaaacag gaatatcaac ggaaatcggc ttaggttcgc
     2161 gcttacagga taataacaac aggatgacac aaagcgtgga aaaaacctta aatttcattt
     2221 tcagcatttg aggcacaaag gtaagataag ttttgcaatc aaaggtcctg aagccattct
     2281 tctgcctgac gattgaatcc ttttggcact tcat
//
LOCUS       DAOQMC010000319         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_30229_length_2288_cov_5.028213, whole genome shotgun sequence.
ACCESSION   DAOQMC010000319 DAOQMC010000000
VERSION     DAOQMC010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30229_length_2288_cov_5.028213"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1720)
                     /locus_tag="PKV31_09850"
     CDS             complement(113..1720)
                     /locus_tag="PKV31_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB93204.1"
                     /translation="MRLINILLLFSIVTFYCMGLHGQVLEGSGSAPGSYLIQFDSGDA
                     AASFSKRMTTERKGVTLESCGINSNILLVTGVDLDWDELTLLVSNSDGVIAFQRDYYL
                     ENRSKEPNDPWFKTKQWDMKTIKGPEAWEITTGGVSPLGHQIVVGVIDNGFFVNNADL
                     HANLYVNPGEIPDNGIDDDGNGYIDDVRGVNIGNGTGIHAEASHGTGVLGIIGAKGNN
                     SYAIAGVNWNVKMLPVSNAVNRVSDLIKGYEYIKEFRRKFNKSGGLSGAFVVATNLSA
                     GISKSWPSDQPIWCNMYDTLGKYGIVNVGATANQNVNVDVEGDLPSTCESEFLIVVTN
                     TDQSDEKVISAGYGSKSVDLGAPGDGSTSAQTNSDFGAFGGTSCATPHVTGAVALLYS
                     APNTGLEGFYMKYPEEAARSVKKAILNNVDQRNSLQGVTVTGGRLNLFEMIKGYKANN
                     FTPAQESIGLWPNPVQNRLTLASSVHFGQESLLEIFNASGEIVLRKQIRQVNGEYAVD
                     VSGFKPGAYLIRLVGNNVVGNGRFVKL"
     gene            complement(1941..>2288)
                     /gene="lepB"
                     /locus_tag="PKV31_09855"
     CDS             complement(1941..>2288)
                     /gene="lepB"
                     /locus_tag="PKV31_09855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNB93205.1"
                     /translation="YGAVWIPKKGVTINISEDNIAFYRRVISVYEKNNLEEKNGKFYI
                     NGTETNQYTFKQDYYWMMGDNRDNSEDSRFWGFVPEENIVGKPLFIFFSKYSDMNGSK
                     IRWNRIFSGANKF"
BASE COUNT          699 a          547 c          380 g          662 t
ORIGIN      
        1 cctcggcatt ataccaaaaa tttatagact attatataaa tcatcttggt ataaattaat
       61 ggaataaggg ccaatttggt ggagttccac acaaacaatt tggatagcgt cgttaaagtt
      121 ttacaaatcg accgtttccg acaacattat ttccaacaag tcgtatcaaa taggctcccg
      181 gcttaaaccc ggatacatca acagcatatt caccattgac ttgccggatc tgctttctga
      241 gtacaatttc tccggaagca ttgaagattt caagcagact ttcttgacca aaatgaaccg
      301 aagaggccaa tgtcaaccga ttttggaccg gattaggcca gagaccaata ctttcctgcg
      361 caggagtaaa attattggcc ttataacctt taatcatttc aaataagttc aatctgccac
      421 cggtaacggt aacaccttgc agagagttac gctgatccac attattcaaa atagcttttt
      481 taacagagcg ggctgcttcc tctggatatt tcatgtaaaa gccctcaaga ccggtattgg
      541 gtgcagaata aagtaaagct acggcacccg taacatgtgg cgtggcacag gatgtaccac
      601 caaaagcacc aaaatcggaa ttcgtttgag cagatgtgga gccatcaccg ggggcaccca
      661 gatcgacgga tttactccca taacctgcac ttatcacctt ttcatctgac tgatcagtat
      721 tggtcaccac tattaaaaac tcactctcac aggtacttgg caagtctcct tctacgtcaa
      781 cgttgacatt ctgattggcc gttgcaccga cattaactat accatattta cccagtgtat
      841 catacatatt acaccatata ggttgatctg aaggccagct tttgctaata ccggcagaaa
      901 gattggtcgc cacaacaaat gcgccgctca agccaccgct tttattaaac ttccttctga
      961 actctttgat atactcatat cctttaatca gatcggagac acgattcact gcattggata
     1021 cgggaagcat tttaacattc cagtttacgc ctgcaatagc atacgaatta ttacccttag
     1081 caccaataat acccagaact cctgtaccat gactagcctc agcatgaatc cccgtaccat
     1141 tgcctatatt gacgcctctg acatcatcga tatatccatt gccatcatcg tcaattccat
     1201 tatcaggaat ttctccaggg ttgacatata aattggcgtg taaatctgca ttattgacaa
     1261 aaaaaccgtt atctataacc cctacaacga tctgatgccc cagtggagaa acacctccgg
     1321 tagtaatttc ccatgcctca ggccccttga tagtcttcat atcccattgc ttggttttaa
     1381 accaggggtc attaggttct ttcgacctat tctcaagata ataatctctt tgaaaagcta
     1441 taactccatc agaatttgaa actagcaagg taagttcgtc ccaatccaaa tcgactcctg
     1501 ttaccagtag gatgttacta ttgataccac aggattctaa tgtcactcct tttctttcag
     1561 tagtcattct tttggaaaag gaagccgcag catcacccga atcaaattga atcaaataac
     1621 tgccgggggc actccctgat ccttccaata cttgaccatg caggcccata cagtaaaatg
     1681 tcactattga aaataaaagc agaatattaa tgagtcgcat atatatagaa attcattaat
     1741 gcaaatctaa tcaaaaaggt accagaaggc aaaaaaataa tattttttca caaaaaggaa
     1801 ccattctata tcaaccgacc caaataatgc catcctgatc aggtatgtat ggatactccc
     1861 tccgggtgct tacctccagg caaccattat ttaaaagaat ttccggtata atgacaactt
     1921 tgtcgaccct tctggccgtg ttagaattta ttggccccgg aaaatattct gttccacctg
     1981 attttacttc cattcatatc actgtatttg gaaaaaaaga tgaaaagagg tttgcctaca
     2041 atattctcct caggcacgaa gccccagaac ctggaatctt cagaattatc cctgttatcg
     2101 cccatcatcc aataataatc ctgtttgaag gtatattggt ttgtttcagt gccgttgata
     2161 taaaactttc catttttttc ctccagatta ttcttctcat agacactgat aactctccga
     2221 taaaaagcaa tgttatcctc actgatattt attgtaacac ccttctttgg tatccataca
     2281 gcaccata
//
LOCUS       DAOQMC010000320         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_30340_length_2284_cov_4.480036, whole genome shotgun sequence.
ACCESSION   DAOQMC010000320 DAOQMC010000000
VERSION     DAOQMC010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30340_length_2284_cov_4.480036"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKV31_09860"
     CDS             <1..486
                     /locus_tag="PKV31_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNB93206.1"
                     /translation="KELKVFDAGICKFGIQNCYDIEFAIGSHYLTQAGAEVILVPSCT
                     ETIRGATRVHVGARARAMEFQCYTVVAQIIGEAQWSPAVDVNYGYPAIYSTPDRKVGK
                     EGIMAIGEAQKPGWLSRELNLSLLNYVRTEGQVLNYRDHQRLLMNLEGEDIEVMVVDM
                     I"
     gene            complement(905..>2284)
                     /locus_tag="PKV31_09865"
     CDS             complement(905..>2284)
                     /locus_tag="PKV31_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HNB93207.1"
                     /translation="KIIFFLLSISVIYSCKITLKGSKPISLPSNNIKTDSMAADVKAQ
                     MLHCWNTYKKYAWGHDEVRPLAMSSKNYYNEPLMLTPISAYSTFRMMGLDQEAAECKE
                     LIMSRLNFDRDITVNHAEVVHVVLGGLLNAYEADNDEQWLDMAADLGNRLIPAFNAPW
                     GLCYRQVNLKTGTTSGDICTPNEIGGMLMEYGTLSKYTGDAKYFDIAMKGTKSLYNRK
                     SKINLLGSAMDVRSGTWTNPESNIMEGSDIWIDDLYKAAVLFNNAELYAMFTTNITAI
                     IKYLREYTESGTWYENGNMNYGTQVHPEFSAQSCSFPALLMLYGNKTEADLVFESVIR
                     YWSEYGMAPELMNYKSKQVISASYLLRPQPLLSAAVLNKYGNKSRYLQAGNYFYTQLV
                     KFCRNDDNGFVALRDVRTLEKMDQLDYYFFGATLKYAYMLYNSEAARYLDTHVFNASG
                     MMFKKPSNE"
BASE COUNT          622 a          438 c          535 g          689 t
ORIGIN      
        1 aaggaactga aggtttttga tgcggggatt tgtaaattcg gtatacaaaa ctgttatgat
       61 attgagtttg ctattggaag tcattatctg acgcaggccg gagcggaagt tattctggtg
      121 ccaagctgca cggaaactat ccgtggtgcg acgcgagtac atgtgggagc acgtgccaga
      181 gccatggaat ttcaatgcta cacagttgtg gcacaaatta tcggtgaggc acaatggtca
      241 ccggcagtgg atgtcaatta cggctatccg gcgatttaca gtacaccgga cagaaaggtc
      301 ggcaaggaag gtataatggc cattggcgag gctcaaaaac cgggatggct gagccgtgag
      361 cttaaccttt cattgttgaa ttatgtacgg acagaaggtc aggtattgaa ttacagggat
      421 catcaacgat tgctcatgaa tttggaaggc gaagatattg aagtgatggt ggtggatatg
      481 atatgatgga tgtggaaaga tattgattga tttgattgac gccaatgatc ccggattaat
      541 tttataggat gtaaagacat ttaagtcgaa ataaagatta ggatatttac cttgaaaaaa
      601 cttatctttt tctgcttgat cttcctgtaa tgccggtaat atatttgccg gaaaggtagg
      661 taattgcaag tgcaactcag gaaagtgtat gcgtgttcct gtcaagctct gaccaaaggt
      721 ttacctgacc ctataaacac ctgcatcagc gagtcgaaag gttgcaggta aaagactgca
      781 atggaatctg ataaaggatt ttgtgaccgt gaaaggcaat tccaacaaaa taaaagcagt
      841 tattttagaa ctgaatagct accagatata atattcattt gcttattgtc ctgagtgagt
      901 atgtttattc atttgatggt tttttgaaca tcatccccga cgcattgaat acgtgcgtat
      961 ccagatatcg ggcggcttcg ctgttgtaca acatatacgc atacttcaag gtcgctccga
     1021 agaagtaata atctaattga tccatctttt ccaatgttct gacatcgcgt aatgcaacga
     1081 aaccattgtc atcattgcgg caaaacttga ccagttgtgt atagaaataa tttccggcct
     1141 gtagatatcg agatttatta ccgtatttgt ttaacacagc tgccgaaagc aagggttgtg
     1201 gtctgagaag ataggaggcc gaaattacct gtttgctttt ataattcatc aattccggtg
     1261 ccataccata ttcggaccag tacctgatta cagactcaaa aaccaaatct gcttcggttt
     1321 tattgccata cagcataagc agggcgggaa agctacagga ttgggcggaa aactcaggat
     1381 gtacctgtgt accgtaattc atatttccgt tttcgtacca ggtgccggat tcagtgtatt
     1441 cgcgaagata ctttatgata gccgtgatgt tggttgtaaa catagcgtat agctctgcat
     1501 tattgaatag cacggcggct ttgtacaagt catcgatcca tatatccgag ccttccatga
     1561 tgttggattc cggattggtc catgtgccgg atcttacatc cattgcggat cccaggagat
     1621 tgattttgga ctttcggtta tagagtgatt ttgtaccttt catggctata tcaaaatatt
     1681 ttgcatctcc ggtatatttg cttagagtcc cgtattccat cagcatgccg cctatttcat
     1741 tgggtgtaca tatatctcca cttgttgttc cggttttcag gttgacttgt cggtaacaga
     1801 gtccccaggg tgcattgaat gccgggatga gcctattgcc cagatcagcg gccatatcca
     1861 gccattgctc gtcattgtct gcctcgtatg cattgagcag ccctccgagg acaacatgca
     1921 caacctctgc atggttgact gtaatgtccc tgtcaaagtt taatctactc atgatcaatt
     1981 ctttacattc agcggcttcc tggtctaatc ccatcattct gaaggtggaa taggcactga
     2041 ttggggtgag catcaagggt tcgttgtaat aattttttga agacattgcc agcggtctca
     2101 cttcatcatg tccccaggca tatttcttgt aggtattcca gcaatggagc atttgagcct
     2161 tgacatcagc tgccatcgaa tctgttttaa tattgttgga aggcaacgat attggttttg
     2221 aaccttttaa ggtgatttta caagagtaaa tgacgctgat ggatagcagg aaaaatatta
     2281 tttt
//
LOCUS       DAOQMC010000321         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_30656_length_2272_cov_3.815968, whole genome shotgun sequence.
ACCESSION   DAOQMC010000321 DAOQMC010000000
VERSION     DAOQMC010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30656_length_2272_cov_3.815968"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1064)
                     /locus_tag="PKV31_09870"
     CDS             complement(12..1064)
                     /locus_tag="PKV31_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93208.1"
                     /translation="MMMKRYLSILLLTFTIMAVKAQGVMTPELLWSLGRVNGAFLHDN
                     NKSICYNVTTYDIKTGKRTNFISSMSIDGKNIKSISKDQPGDNPILLPDGNILYSANG
                     ELTSSGKVPDGLKDADNLLFNAKTGHAAWSQSIKIDKTAADMYPDYAATSARIYDDLM
                     YRHWAQWSDGTYNHVFVGKLQPDGSYKGIDIMPGEPHDAPTKPFGGSDDMAWHPDGNL
                     LAYVSVKKTGKEYAVSTNSDIYFYDLNTGKTTNFTLGMMGYDRSPAFSSDGKWLAWTS
                     MPRDGYEADKNSLWIANLTTGKKYDLFQNYDGNVNAFVWAPSNDKLYFSAPSNGLTSL
                     FELKLNIDKRQHIYSW"
     gene            complement(1082..1564)
                     /locus_tag="PKV31_09875"
     CDS             complement(1082..1564)
                     /locus_tag="PKV31_09875"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB93209.1"
                     /translation="MITWRNAKIEDLPTIGLLAGKIWEATYLDNIGRDQLEYMLNLFY
                     APETLLAKLEQGHNLYIINSDEKAVGYLHIESRPEDYFIHKFYIDTTIQNKGIGSHVI
                     KMIYEYIIQNNRPIRLQVNRKNYKAINFYFKNGFTIESVGDFDIGGGYYMNDFVMIKN
                     "
     gene            complement(1685..>2272)
                     /locus_tag="PKV31_09880"
     CDS             complement(1685..>2272)
                     /locus_tag="PKV31_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNB93210.1"
                     /translation="LGMEAVSAHCDIGKDFERKAEEAAEVGLKYLVCPWLGKQPNLDF
                     YRKAAEDFNQKGEICRKYGIRFAYHNHAYSFEKVGGTYPQDLMMKETDPALVDFEMDM
                     YWVHAAHENINHWLEKYKGRFKLCHIKDYSKNPGQDDGLNSVDVGKGVINWKDALTTA
                     VNTGMKYFIVEQEAYPGTTPLEAIRANAKYMMQFL"
BASE COUNT          595 a          471 c          451 g          755 t
ORIGIN      
        1 aaaatctgga tttaccagct gtatatatgt tgccttttgt caatattcag tttcagctcg
       61 aataaggatg taaggccatt cgaaggggcg gagaagtata atttatcatt ggagggcgcc
      121 cacacaaatg cgtttacatt accatcataa ttttggaata aatcatattt ttttccggtt
      181 gttagattgg cgatccataa gctattttta tcggcttcat agccatcacg aggcatggaa
      241 gtccaggcga gccattttcc atctgatgaa aaagcaggac tcctgtcata acccatcatg
      301 ccaagggtaa agttggttgt ctttccggta tttaaatcgt agaagtaaat atccgagtta
      361 gtactcactg cgtattcctt accagtcttt tttacggata cataggccaa aagattacca
      421 tcgggatgcc aggccatatc gtcacttccg ccgaaaggct tggttggagc gtcatgtggt
      481 tcacccggca taatgtcaat acccttataa ctaccatccg gctgtagttt gcccacaaaa
      541 acatgattgt atgtgccatc agaccattgt gcccagtgcc tatacataag gtcgtcatat
      601 atacgcgcac tggttgcagc ataatcggga tacatgtctg ccgctgtctt gtctattttg
      661 atggattgcg accatgctgc atgaccggtt ttggcattga acaacaaatt atcggcatct
      721 tttaatccat cgggaacctt tccggatgaa gtcaattcgc cgtttgcaga atatagaata
      781 tttccatccg ggaggaggat tgggttatca cctggttggt ccttacttat agatttgatg
      841 tttttgccat cgatactcat ggaagaaatg aagttggttc ttttgccggt tttgatgtca
      901 taggtagtga cattatagca gatcgactta ttattgtcat gcaggaaggc tccgtttacc
      961 cttccaagag accatagtaa ttcaggggtc atcacaccct gggctttgac tgccataatg
     1021 gtgaaagtca gtaaaagaat ggataagtac cttttcatca tcatattttt tagtttgaaa
     1081 attaattttt tatcatcaca aaatcattca tataataacc tccgccaata tcaaaatccc
     1141 ctacactttc tatcgtgaag ccatttttaa aataaaaatt tatcgccttg tagttttttc
     1201 tattgacctg gagcctgata ggccgattgt tttgaatgat gtattcatat atcattttaa
     1261 taacatggga gccaatgccc ttgttttgaa ttgttgtgtc aatataaaat ttatgaatga
     1321 agtaatcttc cgggcgagat tcaatatgta gataacctac agctttttca tcggaattga
     1381 tgatatatag gttatgacct tgttcaagct ttgccaataa tgtttcagga gcataaaaca
     1441 gattcagcat atattccaat tggtcacgtc cgatgttatc caggtaggtt gcttcccaga
     1501 tctttccggc tagtagtcct attgtaggta gatcctctat tttagcattt ctccatgtta
     1561 tcatattgcg ctgataagct ttcaggtttt aagtacaatc cgattatccg atatggataa
     1621 aacataataa attgctcaag gttactatac accggaatga tgacccattc aggtctttat
     1681 atcatcacag gaattgcatc atgtattttg cattggctct gatagcttcc agtggtgtgg
     1741 ttcccgggta tgcttcctgt tccacaatga agtatttcat gcctgtgtta acggcagttg
     1801 taagggcgtc tttccagttg atgacacctt tgccgacatc cacagagttc aagccgtcat
     1861 cttgtcctgg gtttttgcta taatccttga tgtggcaaag cttaaatctg cctttatatt
     1921 tttccagcca gtggttgata ttctcatgtg ctgcatgcac ccaatacata tccatttcaa
     1981 agtcgactaa tgcaggatct gtttccttca tcattaagtc ctgcggatat gttccaccta
     2041 ccttttcaaa ggaataggca tgattgtggt aggcaaatcg gataccgtat ttcctgcata
     2101 tttctccttt ttgattgaaa tcttctgccg cttttctata gaagtcaaga ttgggttgtt
     2161 tgccaagcca tgggcatacc aggtatttca agccgacttc tgctgcttct tcggcctttc
     2221 tttcaaagtc tttaccgata tcgcagtgtg ccgatactgc ctccatgccc aa
//
LOCUS       DAOQMC010000322         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_30943_length_2260_cov_6.247166, whole genome shotgun sequence.
ACCESSION   DAOQMC010000322 DAOQMC010000000
VERSION     DAOQMC010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30943_length_2260_cov_6.247166"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="PKV31_09885"
     CDS             complement(<1..1753)
                     /locus_tag="PKV31_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93211.1"
                     /translation="MKFYFLFFLVLTLTLTNAQPSTWSSSGLGGGGSLFAPSISPVNS
                     SIMYMQCDMSEVFHTSDAGIHWQPIHFKQLISTGGTHPVVFTNDPSILYTVNYGFLDD
                     TRFPVKSVDGGNTWNQLASDPTGGDVWFISSDPNSTQRIIISSYTAMYLSVDGGNTFS
                     LKYNHGSDFLISGVFWDGNDIYIGTQIGLIVSHDGGVSFALDSSPGIPAGYGFMSFTG
                     SKSNGITRLMGTIAAQDDLYPGVNALDIDIYSSIIRIDYGTTGWTNAVGGINPTHNLF
                     YIASALNNSSVFYVGGTNPNTSYPVIYKTSDGGNSWAEVFLTVNNQNISTGYSGYQGD
                     EDWWYGEIVFGLAVAPNDPQTVIYTDFGFAHISTDGGTNWRQSYVASEDQNPAGSPTA
                     KGKAYGNNGLENTSCWNMHWCSANEDIILASYTDITATKSIDGGIKWSFDFNGINYNT
                     VYHIIEHPTNGTLYAATSSVHDIYQSTYLTDSRIDGGTGAILYSTDQGLHWNLLHNFN
                     HPVIWLAIDPKNNNRMYASVVHSTAGGIYLTSDLNNGSLSNWSSTSKPARTEGHPYNV
                     FVLNDGTVVTTWSGRRTS"
BASE COUNT          756 a          413 c          499 g          592 t
ORIGIN      
        1 cggaagttct tcggcctgac caggttgtta ccaccgtacc atcattcaaa acaaaaacat
       61 tgtatgggtg accttctgtt cgtgccggct tcgaagtaga tgaccagttt gataaggatc
      121 cattgttaag gtctgaagtt aaataaattc cgccggcagt actatggaca acactggcgt
      181 acatcctgtt gttattttta gggtcaatcg ccagccagat tacaggatga ttaaagttat
      241 gcaacaaatt ccaatgaaga ccctggtcgg tactatatag gatagccccg gtaccgccgt
      301 ctattctgct gtcagtcaga taggtacttt ggtagatatc atggacactg ctcgtcgcag
      361 cgtatagggt accattagtg gggtgttcaa tgatatggta tacggtatta taattgattc
      421 cattaaaatc aaaagaccat tttatgccac catcaatgct tttggttgca gttatatcag
      481 tataggaagc caggattata tcttcattgg cagagcacca atgcatattc cagcaggaag
      541 tattttcaag accattatta ccataggcct taccctttgc agtgggactt ccggcaggat
      601 tttgatcttc ggaagcgaca tacgactgtc gccaattagt gcctccatct gtcgaaatat
      661 gagcaaagcc aaagtcagta taaattacag tctgaggatc attaggtgcc acagccaagc
      721 cgaagacaat ctctccatac caccagtctt catcaccctg gtaaccggaa tatccagttg
      781 aaatattttg attgttgact gtcaaaaaaa cttcggccca tgaattacca ccatcagaag
      841 tcttatatat caccggatag gaggtattcg gattggtgcc accaacataa aatacagagg
      901 aattatttaa tgctgaggca atataaaaca aattgtgagt cggattgatg ccaccgactg
      961 cattggtcca gccggttgtt ccatagtcta ttctgattat ggaggaatat atatcaatat
     1021 ccaaggcatt aacaccagga taaagatcgt cttgtgcggc gatggtgccc ataagcctcg
     1081 taataccatt gctttttgaa cctgtaaagc tcataaagcc atatcctgcg ggtatgccgg
     1141 ggcttgaatc cagggcaaag ctgacgcctc catcatgaga aacaatcaat ccgatctgtg
     1201 tgcctatata aatatcgttg ccgtcccaga aaactccaga aataagaaag tcggagccat
     1261 gattgtactt aaggctgaag gtattacctc catcaactga aagatacatg gcggtatagg
     1321 atgagatgat aatacgctgt gtagaattag ggtctgaaga aataaaccaa acatcaccac
     1381 cggtaggatc tgaagccagt tgattccatg tattgcctcc atcaactgat ttgacgggaa
     1441 atctagtatc atccaggaag ccataattga ctgtataaag aatggaagga tcattggtga
     1501 aaacaacagg gtgagtaccg ccagtagaaa tcaattgttt gaagtgaatt ggttgccaat
     1561 ggatacctgc atccgaagta tgaaaaactt ctgacatatc acattgcata tacataatag
     1621 aggagttgac aggactaatg gatggtgcaa aaagagaacc tccaccgccc aggccggatg
     1681 aagaccaggt agatggctgg gcattagtca aggtcaaagt taaaacgagg aagaagagga
     1741 agtaaaattt catggcaaaa tgaatttgag tgttagtaag aatggataag aattaaaaaa
     1801 aatgacacaa cagaaatctt taaaaaacac gaatctggat tgaattccgg attatcaaga
     1861 aacagtcatg acaaaaaaaa ggatcatcag gagatgccct caaaaataca aaaaaagatt
     1921 tttgagaagt gaaatatata taaaatttta aaaaatacta aaaaacagtg ataaacaaaa
     1981 aatggtaagt tctaagtatg ccgaatagca tttaaaaggc tggtagggct gggagttgag
     2041 agcagtggaa gtgtgtagat aattaataac tgcaaagtat gcaaggatgg atgattgtag
     2101 agatgtagac gaagttaaag taacaaatca gagaaataaa taattcaatt gtgacaaaag
     2161 taattgttca gtggggaata tctattatta ctttcctacc tactacgatt tttggggaag
     2221 ttgtgggtgg tgccgcctcc gaagtaggta gatgattaat
//
LOCUS       DAOQMC010000323         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_31144_length_2252_cov_5.939463, whole genome shotgun sequence.
ACCESSION   DAOQMC010000323 DAOQMC010000000
VERSION     DAOQMC010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31144_length_2252_cov_5.939463"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKV31_09890"
     CDS             <1..228
                     /locus_tag="PKV31_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93212.1"
                     /translation="DEMFDKLMEVDGLHNIVADKANNRAEVIILMEVVLHGLAELNMI
                     TREMLDDKISFNDSLADMFDDLLNTDNEDDY"
     gene            313..936
                     /locus_tag="PKV31_09895"
     CDS             313..936
                     /locus_tag="PKV31_09895"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB93213.1"
                     /translation="MLIEINPLNPDSRAINSVVEKMKNGAVIIYPTDSVYALGCDPES
                     KKGVEKLYQLKNSDPSKSPLTFICDTISMASAYSSQISNSNFRFIKDHTPGPFTFILP
                     ASNNLPKILHVKNKTYGFRIPDHRIPQELIKALGRPILSASLKSIEREDEDQFYMYPE
                     DIYSDYSLLVDLVIDGGPGSLEQSTVVDLTDGTPVVIREGKGNVETE"
     gene            1037..>2252
                     /locus_tag="PKV31_09900"
     CDS             1037..>2252
                     /locus_tag="PKV31_09900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB93214.1"
                     /translation="MKILQLCKKFPFPLIDGESLAVNYLSRSLAELGCDITLLTMNTS
                     KHYYQLEKTVPAELKHYRQIYHVEVNNDITIKGAFLNLFSSESYHISRYQSKLFVEKL
                     IEILKKEQFDVIHLETLYLTQFIDVIRKYSNALVVLRSHNVEFEIWERLTENQMSQVK
                     KFYLQYLTRKLKRFEIRKLQEIDLLLAITQRDLKTFRSYGYLKAAKVVPIGLDTSDYL
                     AEEKDVFSNPSMSFIGSLDWIPNMEGLDWFLNDIWPRISSKYPELTFHVAGRNMSTNI
                     SKRKIPNVFFHGEVSDARRFINAHPITVVPLLSGSGMRVKILEGMFLGRIVVTTSLGL
                     EGIDAQHKNQVLIAETADEFMKAIDFCFEHPVQTQNISRRAQVFAARNYDNLEIGKEL
                     LSFYRMKKESVPT"
BASE COUNT          703 a          418 c          482 g          649 t
ORIGIN      
        1 gatgagatgt ttgataagct gatggaagtg gatggcttac ataatatcgt agcagataag
       61 gcaaataatc gtgcggaggt aatcatactc atggaagtcg tcttgcatgg actggccgaa
      121 ttgaacatga taacccggga aatgctggat gacaagatca gtttcaacga ctcattggca
      181 gatatgtttg acgacctcct taatacggat aatgaggatg attattaata gctgacaaat
      241 gaagaattga attgacttct tagccggttt tattttagaa aaatattaat gtatatataa
      301 aaaataaatg acatgttgat tgaaataaat ccactgaacc ctgattcaag ggcaataaat
      361 agcgttgttg aaaaaatgaa gaacggagct gtcatcattt acccgacaga ttcagtttat
      421 gcgctgggct gtgacccgga aagcaagaaa ggtgtggaaa aactttatca gttgaaaaat
      481 tccgatccat ccaaaagccc tctgacattt atttgtgata ccatttccat ggcctctgcc
      541 tattctagcc agatatccaa ttccaatttc cggtttatca aggatcatac tcccggacct
      601 tttacattca ttctgcctgc ttctaacaat ctgcctaaaa tacttcatgt gaagaacaag
      661 acctacggat tcaggatacc cgatcacagg ataccgcagg aactgattaa agcccttggc
      721 cggcctatat tgtcagcatc tttgaagtcc atagaaaggg aagacgagga tcagttctat
      781 atgtatccgg aagatattta ttccgattat tcactgttgg tggaccttgt tatcgacgga
      841 ggtccgggtt ctctggagca aagtacggtg gtggatctta ccgatggtac tccggttgta
      901 atacgggaag gaaaaggaaa tgtcgaaaca gaataagttg tatataaaaa tttaactttc
      961 gacaatttag atatttcctg ttgcgttaca tcaaggataa ttagattatt cttaatttgc
     1021 atcccgaaaa tttggtatga agatattaca actctgtaaa aaatttcctt ttccattgat
     1081 cgacggagaa tctctggctg tgaactacct gagtaggagt cttgctgagc tgggttgtga
     1141 tatcacctta ctgaccatga atacctccaa gcactactat caactggaaa aaacggtacc
     1201 ggccgagttg aagcattata ggcagattta ccatgtggag gtcaataatg atattacaat
     1261 caaaggggca tttttaaacc ttttctcgtc agagtcttat cacatcagcc gatatcaaag
     1321 taaactgttt gtcgaaaagt tgattgaaat tttaaagaaa gagcagtttg atgtcataca
     1381 tcttgagaca ttgtatttga cgcagtttat tgatgtaatc agaaagtata gtaatgcgct
     1441 tgtcgtgcta agatcccaca atgtagaatt tgagatctgg gagcgtctca cagaaaatca
     1501 gatgagtcag gtcaagaagt tctatcttca gtatctgacc agaaaattaa aacgctttga
     1561 gatccgaaaa ttacaggaaa tcgacctttt gcttgccata acacaacgtg atttgaagac
     1621 cttccggtca tacggatatt taaaagcagc taaggtcgtg ccaatcggtc tcgatacctc
     1681 agattatctc gctgaagaaa aagacgtatt tagtaatcca tcgatgagct ttattggatc
     1741 ccttgattgg atacccaata tggaagggct tgactggttc cttaatgaca tttggccacg
     1801 catatcttca aaatatccgg aactgacctt tcatgttgca ggcaggaata tgtcaaccaa
     1861 tatatcaaaa agaaagattc ccaatgtttt ttttcatggc gaggtcagtg atgccaggag
     1921 atttatcaat gctcacccaa ttaccgtcgt accactactt tcaggcagcg gcatgagagt
     1981 aaagattctg gaaggaatgt ttttgggacg gattgtcgtt accacttcgt tgggattgga
     2041 aggtatcgat gcacaacaca aaaaccaggt cctcattgct gaaactgccg atgagtttat
     2101 gaaagcgata gatttctgtt ttgaacatcc ggttcagact cagaatatca gccgcagggc
     2161 tcaggtattt gccgcccgaa attatgataa tctggagatt ggaaaggagc tattatcctt
     2221 ttacagaatg aagaaggaat cagttccgac at
//
LOCUS       DAOQMC010000324         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_32246_length_2207_cov_4.078532, whole genome shotgun sequence.
ACCESSION   DAOQMC010000324 DAOQMC010000000
VERSION     DAOQMC010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32246_length_2207_cov_4.078532"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..2056)
                     /locus_tag="PKV31_09905"
     CDS             complement(74..2056)
                     /locus_tag="PKV31_09905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNB93215.1"
                     /translation="MDPNSNIPATSTRLTGTPLILARLGWWSITILTFIDLSAALYTI
                     FIEKPVLEKPLWFLYDDEIARLNQLGISPDFYVGYITTLAAISVLMFFAVACLIFWRR
                     SDDWQAILISQMGITIGIGTGWAIDGPLIDTHPNWLLLPVFISLIPVIGGVIIFFTFP
                     DGRFIPGWTRWLVLPLSLFFLGISLIAPSGTNVITKSIFFTKMGVIAFSVLAQVYRYR
                     RFSTQFQRQQTKWIIYGFILMILTLITHAASSPYYFPRLDDLSAKIYFFVYLPIIGVI
                     PYYFPLFGIVFSVLRYRLWDIDFIINRSLVYGVLTALLVTLFGGSLFMVSWLFQNFSG
                     GPIVAVAVSAAIFGFIFQPTRRQVQRFVDQRFYRIEIDYQKTQPDLPNSGKTEILRHA
                     RVGIYENLELIGRGGMADVYKSTHPTLGTPVAIKILPERLSIENEFRRRFAREALVIS
                     KLQHPNIVRIFDSGEQNGRYYMVMEYLPGRDLDQLIKNNGKLSLTQALPLIQQIASAL
                     DYAHAQGFVHRDIKPSNVLLDSHGTRAVLTDFGIAKITDAHTVMTITGYMLGTFDYIA
                     PEQIQESAHVDGKADIYALGVMVYQMLTGVLPFKHVNPGALLIAHMNQPAPNMCETMS
                     DIPYHVGSAVQRAMAKKPEERFATATEFAMEINNKV"
BASE COUNT          678 a          450 c          531 g          548 t
ORIGIN      
        1 atcgtctgcg tttgcattgc ggagaattaa gcggggagtt tcgagaatgt tcatttgaaa
       61 tggcacctgt tgattaaacc ttattgttaa tttccatggc aaattctgtt gccgttgcaa
      121 atcgttcttc tggttttttt gccatggcac gttgaaccgc agaacccaca tgataaggaa
      181 tgtcagacat agtttcacac atattgggtg caggctgatt catgtgggcg atcagcaagg
      241 caccggggtt gacgtgtttg aagggcagta caccggtcag catttgatag accattacac
      301 caagagcgta aatatctgct ttgccatcca catgggccga ttcctgaatt tgttccgggg
      361 caatgtaatc aaaggttccc aacatatatc cagtgatcgt catgaccgta tgtgcatcgg
      421 tgatttttgc aattccgaag tcggtcagta cggcacgagt gccgtgcgaa tccagcagga
      481 cgttggatgg tttgatatcg cgatgtacaa atccttgtgc atgtgcatag tccagtgcag
      541 atgcgatctg ttgaataagc ggtaaagcct gggtgagtga aagttttcca ttgttcttaa
      601 tcaattgatc caagtccctg ccggggagat attccattac catgtaatag cgtccattct
      661 gttcgccgga atcaaaaatg cggacgatgt tggggtgttg aagtttggat ataaccaggg
      721 cttcacgtgc aaaacgtcga cggaattcat tctcgatgga gagtcgttct ggcaggattt
      781 taatcgcaac cggagtaccc agtgtaggat gggtggattt atacacatct gccataccac
      841 cgcgcccgat caattcaaga ttctcatata tacccacccg ggcatgacgc aggatttccg
      901 ttttgccgct atttggaaga tcaggctgtg tcttttggta gtcaatttcg atacgataga
      961 aacgctggtc tacaaaacgt tgaacttgac gacgagtagg ttgaaagatg aaaccaaata
     1021 tcgctgcaga tactgcaact gccactatgg ggccgcctga aaaattttga aatagccaag
     1081 aaaccataaa caaacttccg ccgaaaagcg tgaccagcaa agcagtaagt actccataaa
     1141 ccaacgagcg gttgatgatg aagtcgatat cccataagcg atatcgtaga acggaaaata
     1201 caattccaaa cagcggaaaa taataaggaa tgaccccaat gataggcagg tatacgaaga
     1261 agtaaatttt cgcagataaa tcatcaagcc gtggaaaata atacggagat gatgctgcat
     1321 gggttatgag tgtgaggatc atcaggataa atccatagat gatccacttg gtctgctggc
     1381 gttggaattg ggtggaaaac cgtcgataac ggtagacttg ggctaatacg ctaaaggcga
     1441 tcacgcccat ttttgtgaag aaaatggatt tggtgattac attggtgccg gaaggagcaa
     1501 tcaaagatat tcccagaaaa aacaggctga ggggaagcac aagccagcgt gtccatccgg
     1561 gaatgaagcg cccatctgga aatgtaaaga aaatgatcac acccccaata acaggaatca
     1621 gggagatgaa cacaggcaat aatagccagt tgggatgggt atcaattagg ggaccatcaa
     1681 tagcccaccc tgtacctatt cctattgtga tgcccatctg ggaaattaat attgcctgcc
     1741 aatcatcaga ccgtctccaa aaaatcaggc aggctacagc aaagaacata aggactgaga
     1801 tcgccgccag tgtagtaatg tatccaacat aaaaatcagg cgaaatcccg agctgattca
     1861 agcgagcgat ttcatcgtca tatagaaacc ataaaggctt ttctaatact ggcttttcaa
     1921 tgaagattgt atataaggcc gcagatagat caataaatgt aaggatagta attgaccacc
     1981 aacccagccg tgcgagaatc aaaggcgtgc cggtcagacg tgttgaagta gcagggatat
     2041 tcgaattggg gtccatggtc atattcttta tcctgcctat gatcataaag aaatgggtat
     2101 aaagatttcc agtgaattta ggaaatgatg aaattggaaa gcaaacgtac gccaactata
     2161 caactaatcc aaatgtttga caacaaaaaa gccattccgc tgacaag
//
LOCUS       DAOQMC010000325         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_32299_length_2205_cov_4.754884, whole genome shotgun sequence.
ACCESSION   DAOQMC010000325 DAOQMC010000000
VERSION     DAOQMC010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32299_length_2205_cov_4.754884"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="PKV31_09910"
     CDS             <1..1446
                     /locus_tag="PKV31_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNB93216.1"
                     /translation="ITKPIEKIETDDVLSIRQDEIIPVDSILSKGKALIDYSFVTGES
                     LPVEAKIGDIVYAGGKQSGEKIEVVAIKTVSQSYLTNLWNNNKFKNKSETNSYIDLLS
                     KHFTWILLVLVAITATYWILQERPDIMWNAVTTMFIIACPCALLLTATYTYGRLIRMY
                     GKNGLYLRHHDVLEAMSEVDYIIFDKTGTLTDHQCNLPEYSGITLTIEQKEVLCSLSD
                     ESSHPLSRAISESFTGTDTIKIESYRNTPGKGIEGWHNDNHYKLGSASFVGYTGAEQS
                     FTASVIYYSVNGSILGKFSVRNKYREGIEDMFSRLKDDYRLAILSGDHNGEKKYLDKL
                     IGEGNSSYFACDPQQKYELVKSLQEDGHKVMMIGDGLNDAGALRQSNVGIAVSDSVNN
                     FTPAADGILDGTKVTTIPALMKLSRIGRKIIYFTFGVSLIYNIAGLWFAVQGLLYPVV
                     AAIIMPISSVTIIFLTYFLSGYMGSILKLKD"
     gene            1615..>2205
                     /locus_tag="PKV31_09915"
     CDS             1615..>2205
                     /locus_tag="PKV31_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB93217.1"
                     /translation="MELEKFQYDNEIPKQFAVATIFWGVVGMLIGVIAAFQLAYPGLN
                     LGIEYTTFGRLRPVHTNAVIFAFVGNGIFTAVYYSMPRLLKTEMWSKALGKIHFWGWQ
                     LIIVAAAITLLMGYTTAKEYAELEWPLDIAITLIWVVFGINMFGTILTRRERHLYVAI
                     WFFIASWVTVAMLHIVNSVELPVSLFKSYSWYAGVQD"
BASE COUNT          646 a          417 c          498 g          644 t
ORIGIN      
        1 attaccaagc ctattgaaaa aattgagact gatgacgtcc tttccattcg tcaggatgag
       61 ataattcccg tggatagcat cttatccaaa ggtaaagcat tgatcgatta tagctttgtt
      121 acaggtgaaa gcctcccggt tgaagctaaa atcggcgaca tcgtatatgc aggaggtaaa
      181 caatccggtg aaaaaatcga agtggttgca ataaaaaccg tctcacaaag ctatctgacc
      241 aacctttgga acaacaataa attcaagaat aaatccgaaa ccaattctta catcgactta
      301 cttagcaagc actttacctg gatactactg gttttggttg ctatcacggc aacttactgg
      361 attctccagg aaagacctga tatcatgtgg aacgctgtta ccactatgtt tatcattgcc
      421 tgtccttgtg ccttacttct tacggcaacg tatacctatg gacgcctcat taggatgtat
      481 ggcaagaacg gcctgtacct acggcaccat gatgtgctgg aagcgatgtc ggaagtggat
      541 tatatcattt ttgacaaaac cggcaccttg accgatcacc aatgcaacct gccggaatat
      601 tctggaataa ccctgaccat tgaacaaaag gaagttcttt gctccctttc ggatgaatca
      661 agtcatccgc tcagccgtgc catatccgaa agttttaccg gaactgatac catcaagatt
      721 gaaagctatc gcaatactcc gggaaaaggt atagagggat ggcataatga caatcattac
      781 aaattgggat cagcatcctt cgtgggatat accggtgcag aacaatcttt tacagcatca
      841 gtaatatatt attccgtcaa tggcagtata ttgggcaaat tcagcgtacg gaataaatac
      901 cgtgaaggga tagaagacat gttttcccgg ttgaaagatg actacagact ggcaatattg
      961 agtggagatc acaacggtga aaaaaaatat ctggacaaac tcatcggtga aggcaacagt
     1021 agttactttg cttgtgaccc ccaacagaaa tatgagttag tcaaatcatt acaggaagat
     1081 ggtcataagg tgatgatgat aggcgatggg ttgaatgatg caggtgcatt aagacaaagc
     1141 aacgttggaa ttgcagtgag tgattcagta aataatttta caccggcagc agatggtatt
     1201 ctggatggaa caaaagtgac taccatccct gccttgatga aactatccag gataggaaga
     1261 aagattatct attttacttt tggagtatcc ctgatataca atattgcagg cttatggttc
     1321 gcagtacagg gattgcttta ccccgtagta gcggccatca ttatgcctat aagttcagtt
     1381 actattatat ttttgactta tttcctcagt ggatatatgg ggagcattct taaattaaaa
     1441 gattgactat gagtgtattg ttgttactac taatcatcag catgttgatt tcgggtggat
     1501 ttttagttgc atttttgtgg agtagcaagg acggtcagtt tgaagaccaa ttctcctcag
     1561 ccaatcgcat tttattcgag caaaaaatta aaacgaaaaa taaaaattaa gcacatggaa
     1621 ttagaaaagt ttcagtatga caatgagatt ccgaagcagt ttgcggtagc taccatattc
     1681 tggggtgtcg tcggcatgct tatcggtgtt atcgctgcgt ttcagctggc gtaccctggc
     1741 ctgaatttgg gtattgaata cactacattt ggcaggctac gtccggtaca taccaatgcc
     1801 gtgatttttg catttgtagg taacggcatt tttacagcag tatattactc tatgcccaga
     1861 ttattaaaga cggagatgtg gagtaaggct ttgggcaaga tccacttctg gggttggcag
     1921 ttgattattg tagcagcagc cataacgctg ttgatgggat atactactgc caaggaatat
     1981 gcggaactgg aatggccgct tgatattgcc atcacgttga tctgggttgt ttttggtatc
     2041 aatatgtttg gcactatcct aaccagaagg gagcgacacc tatatgttgc tatctggttt
     2101 tttattgctt catgggttac tgttgccatg ttgcatattg tcaattctgt agagttgcct
     2161 gtaagtcttt tcaagagtta ctcatggtat gccggagtgc aggat
//
LOCUS       DAOQMC010000326         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_32613_length_2192_cov_5.451100, whole genome shotgun sequence.
ACCESSION   DAOQMC010000326 DAOQMC010000000
VERSION     DAOQMC010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32613_length_2192_cov_5.451100"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PKV31_09920"
     CDS             <1..1190
                     /locus_tag="PKV31_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNB93218.1"
                     /translation="GCTSCIGNSGPLPSAIAEAVDKGELVVASVLSGNRNFEARVHPQ
                     VKMNFLMSPMLVVAYALAGRVDIDLINEPIDMDPNNQPVYLRDIWPTRDQIQLTINEC
                     MKQGDFEEVYDIIFDGSTDWQSLEVNLTQNFEWNKDSTYIREAPFFENISATPDEIKD
                     IEGARVLLYLGDSVTTDHISPAGSFKEDSAAGKYLIDNGIERNEFNSYGSRRGNHEVM
                     MRGTFANVRIKNKVADKEGGFSRYMPTGEVMTVFDTAMKYQNDNTPLIILAGKEYGSG
                     SSRDWAAKGTFLLGVKAVIAESYERIHRSNLVGMGVAPLVFVDGQNAQSLGLDGTEVF
                     DIEGLADNLTPHKILNVKAVHSSGKVTEFKVEARLDAPIEIEYYKNQGILQYVLRDYL
                     KNN"
     gene            1292..>2192
                     /locus_tag="PKV31_09925"
     CDS             1292..>2192
                     /locus_tag="PKV31_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93219.1"
                     /translation="MVGDIYDPFESLDFSEETCFLTGEKLDGRNNFTVSAFPEWVMDK
                     YKLNEAYLLMLSGTRLQYKELRMPASKHAFQVVKALDENTQAAFQEGFEAVKHIPDLL
                     LFQWMARVFYGVLYGDCKQGIIEAKEKGQEFRMAPQLKRKFKNLHLMLQSLIRPVEFR
                     NFTPWTICRYQVNISKDIFNYKDEIQHLNFSFGMKDFGIAACLQDNGAVASYHRNLLQ
                     KVEGQPLHPAQFEELYGHLVYANYLMKDFEDYQISLEDDRIVLELAENEATGSIKFAK
                     WEDEIFAQVLTNMWQPWGIPLELV"
BASE COUNT          667 a          377 c          530 g          618 t
ORIGIN      
        1 atggatgtac atcttgtatc gggaattccg gcccacttcc ttccgctatt gcagaggctg
       61 tagataaagg ggaacttgtt gtggcatctg ttctttccgg aaatagaaat tttgaagcta
      121 gggtacatcc acaagtcaag atgaatttct tgatgtcacc tatgcttgta gtagcttatg
      181 cccttgcagg gcgggtcgat atagatttga taaatgagcc gattgacatg gaccctaaca
      241 atcagcctgt atatctcaga gatatttggc ccacacgaga ccaaatccag cttaccatca
      301 atgaatgtat gaagcagggt gattttgaag aggtatatga tattattttc gatggttcta
      361 ctgactggca aagtcttgag gtgaatctta ctcaaaactt tgagtggaat aaagactcta
      421 cctatatcag agaagctcct ttctttgaaa atattagtgc gacgccggat gagatcaaag
      481 atatcgaagg ggccagggtt ttgttatatt tgggagactc tgtcacaact gatcatattt
      541 ctccggcagg ttcatttaag gaagactcgg ctgccggtaa gtatctgatc gataatggca
      601 ttgagcgaaa tgaattcaac tcttacggtt ccagaagagg aaatcatgag gtgatgatga
      661 gaggcacatt tgccaatgta cggatcaaga ataaggttgc tgacaaggaa ggtggattta
      721 gtaggtatat gccaacaggt gaagtaatga cagtatttga tactgctatg aaataccaga
      781 atgacaatac accattgatc atcctggctg gaaaggagta tggatccggt tcttctcgtg
      841 actgggctgc caagggaacc tttcttttag gcgttaaggc agttattgca gaaagctacg
      901 agcgtataca cagaagtaat ctagttggta tgggcgtcgc tcctttggta tttgtggatg
      961 ggcaaaatgc gcaatcacta ggtttagatg gtacggaagt atttgatatt gaaggcttag
     1021 ccgataatct gacaccacat aagatactta atgtaaaggc ggtacattct tccggtaaag
     1081 taactgaatt taaggttgaa gccaggctcg atgctcctat tgaaattgaa tactataaga
     1141 atcaagggat tcttcaatat gtattgagag attatttaaa aaataattag aatagaacct
     1201 attgcgacaa aagcctctgt actcagaggc ttttgtatta atagagatcg ctttcaaaaa
     1261 aatgaattta ctaaattgtc tgataggcta aatggtaggg gatatatacg atccatttga
     1321 atcgcttgat ttctcagaag aaacttgctt cctgaccggt gagaaacttg atggaaggaa
     1381 taacttcacg gtgtcagcat tcccggagtg ggtaatggac aagtataaat tgaatgaagc
     1441 ttatcttctg atgctgagcg gaacaagatt gcagtataag gaactgagga tgccagcctc
     1501 aaaacatgcc tttcaggtgg tgaaggcact cgatgaaaac acacaagcgg cgttccagga
     1561 aggatttgaa gcagtaaagc atataccgga cttgctgtta tttcaatgga tggcacgtgt
     1621 tttctatggc gtattgtatg gggattgtaa acagggaatc atcgaggcaa aggagaaagg
     1681 gcaggaattt cgaatggcac cacaattgaa acggaaattc aagaatctgc atttgatgct
     1741 gcaatcccta atccggccgg tagaattccg taattttact ccatggacaa tctgtcgata
     1801 tcaggtaaat atttcgaagg atatcttcaa ctataaggac gagattcagc atcttaactt
     1861 cagttttggg atgaaagatt ttggcatcgc cgcctgcctt caggacaacg gggctgtggc
     1921 aagctatcat cgaaatctat tgcaaaaggt ggaggggcaa cctcttcatc cagcccagtt
     1981 tgaagaatta tatggtcatc tggtttatgc caattatctg atgaaggatt ttgaagatta
     2041 ccaaatcagt ttagaggacg ataggattgt tttggaatta gctgaaaacg aagcaaccgg
     2101 aagcattaaa tttgccaaat gggaggatga aatattcgct caggtattga ccaatatgtg
     2161 gcaaccttgg ggcattcctt tggaattagt at
//
LOCUS       DAOQMC010000327         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_32723_length_2188_cov_6.327707, whole genome shotgun sequence.
ACCESSION   DAOQMC010000327 DAOQMC010000000
VERSION     DAOQMC010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32723_length_2188_cov_6.327707"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1644
                     /locus_tag="PKV31_09930"
     CDS             115..1644
                     /locus_tag="PKV31_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HNB93220.1"
                     /translation="MIVLGKEALSVSEFEAIVLNKESIGIDAAALRHCIDNHEFLIKF
                     SENKIIYGINTGFGPMAQYRVDDRQRKDLQLNLIRSHCTGSGNLLPDSLVRGILLSRL
                     NTLLLGYSGVHPSVIHLLADFINKEIYPNIREHGSVGASGDLVQLAQVALALIGEGRS
                     NYNGSIMDTSAAFAENNLTPIEVVAREGLAVINGTAAMTGIGLVNNIRSKQLVRLAVM
                     ASCLINEIVNSFDDHFAKVLNDVKKHQGQKEIARMMRENLDGSNCIRQRDSIYYNNIP
                     ENAVFTDKVQEYYSLRCVPQILGPVMDTINYSEKILMDEINSVNDNPVISHEEEYVFH
                     GGNFHGDYISLEMDKLKIAITRLTMLMERQLNFLLSDNLNQKLPPFVNLGKPGFNFGL
                     QGAQFTATSTTAENQTLSFPMYVHSISCNKDNQDIVSMGSNSALLARKVIENAFEVIS
                     IHLMTIVTAIEYLHIQDKLAPATKKFYLSIREFLPPVVEDRPLSDIIDKATRKLKDFD
                     F"
     gene            1654..>2188
                     /locus_tag="PKV31_09935"
     CDS             1654..>2188
                     /locus_tag="PKV31_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB93221.1"
                     /translation="MNKYALVTGGSRGIGRAICMRLAEEGYNIIATYKSNDDAANELL
                     MLLNQNGRDHHLLKYDVADPRQTEAVLNDWIASHPGAEIDILINNSGIRKDNLLIFMP
                     ENDWNEVINTNLNSFYYTTKIVSSGMVKRRKGRIINIVSLSGIKGMPGQTNYSAPKGG
                     LIAATKALAQELAKRNIT"
BASE COUNT          678 a          400 c          472 g          638 t
ORIGIN      
        1 atacaaacgg cacaattggt tctggcatta tcattgaacc gaatattttt caattttaac
       61 atttatcact tttttgtagt tttgcgcctc tttaataccc aagaaatatt tataatgatc
      121 gtactaggaa aggaagcgtt gtcggtttca gagtttgaag cgattgttct caataaggaa
      181 agcattggga ttgatgctgc tgctttgaga cattgcattg ataatcatga atttttaata
      241 aagttttccg aaaataaaat aatatatggg atcaataccg gttttggccc catggctcaa
      301 taccgcgttg atgacaggca aaggaaggat ttgcagctta atttgatcag atctcattgt
      361 accggaagcg ggaacttatt gcctgattca ttggtccggg ggatattatt gtccaggttg
      421 aatacccttt tactgggtta cagtggcgta cacccttctg ttatccatct tttagctgat
      481 tttatcaaca aggaaatata tcccaatatc cgggaacatg gaagtgtcgg ggcaagcggt
      541 gatcttgttc aactggcaca agtcgcttta gctttgattg gcgaaggaag aagtaattac
      601 aatggaagta taatggatac tagtgctgcc tttgcagaga ataaccttac tccgattgag
      661 gttgtagcac gtgaaggcct tgccgttatc aatggaactg ccgctatgac cggcatagga
      721 ttggtcaata atatcaggtc taaacaactt gttaggcttg ctgtgatggc ttcttgcctt
      781 atcaatgaaa ttgtcaattc atttgatgac cattttgcca aggtattgaa tgatgtcaaa
      841 aaacatcagg gccagaaaga aattgcacgg atgatgcgtg agaatcttga tggtagcaat
      901 tgcatcagac aacgggatag tatttattac aataacatac ccgagaacgc tgtttttaca
      961 gataaagtgc aggaatatta ttcgttgagg tgtgtgcctc aaatcctcgg tcctgttatg
     1021 gacactataa attattcaga aaaaatcctg atggacgaaa taaactccgt caatgacaat
     1081 ccggtaatct cacatgagga ggaatatgtc ttccatggag gaaatttcca tggtgactat
     1141 atttctctgg aaatggacaa gttaaagatt gctattacca gattgactat gttgatggaa
     1201 cgtcagttaa atttcctgct gagtgataac ttgaatcaaa agcttcctcc ctttgtgaat
     1261 cttggcaaac cgggattcaa tttcggtctt cagggagcac aatttacagc aacctccaca
     1321 acagcagaga atcaaaccct gtcattcccg atgtatgtcc atagtatttc ttgtaataag
     1381 gataaccagg atattgtaag catgggtagt aattcggctt tattagcccg aaaggttata
     1441 gagaatgcct ttgaggtaat atccatccat ttgatgacca tcgtcacggc tattgaatat
     1501 cttcatatcc aagataaatt agctccggca acaaagaagt tttacctaag tatcagggag
     1561 tttcttcctc ctgttgttga agacagacct ttatctgata tcatcgacaa ggcaacgaga
     1621 aaattaaaag atttcgattt ttaaatgtcc gaaatgaata aatatgcact ggttacaggt
     1681 ggcagccgtg gtataggcag ggcaatctgc atgcgacttg ctgaagaagg atacaacatc
     1741 attgccacct acaagagtaa tgatgatgca gccaatgaat tgttgatgct tcttaatcag
     1801 aatggcagag accatcatct tttgaagtat gatgttgccg atccaagaca aacagaagct
     1861 gtattgaatg attggatagc gagccatccg ggagcggaaa tagacatact gattaataac
     1921 tcagggatca gaaaggacaa cttgctgatc tttatgccgg aaaatgactg gaatgaagtc
     1981 atcaatacca atctgaactc tttttattat actaccaaaa ttgtctcatc gggaatggta
     2041 aaaagaagaa aggggcgcat catcaatatt gtttctcttt caggaatcaa aggaatgccc
     2101 gggcagacca attattcagc acccaagggt ggtcttatag ctgcaaccaa agctcttgca
     2161 caggaactgg ccaaaagaaa tattactg
//
LOCUS       DAOQMC010000328         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_33889_length_2148_cov_4.939322, whole genome shotgun sequence.
ACCESSION   DAOQMC010000328 DAOQMC010000000
VERSION     DAOQMC010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33889_length_2148_cov_4.939322"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PKV31_09940"
     CDS             complement(<1..800)
                     /locus_tag="PKV31_09940"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNB93222.1"
                     /translation="MASTKAFPRFPQVRPSLHSELRRRVSLYFEENKIRQTGNANLYW
                     KAAILLTLFALTVGHLFIFQPHWAIAYSECILLGLLVATIGFNIMHDGSHGSFSTHQS
                     SNRLASYTMSMLGASHFFWNIKHNVIHHTYTNVDDLDDDIEVGKLMRLAPSQTRLPFH
                     KYQHIYFPALYMVMYLYWVFAADYKKYFKMKIGEFPITNMKMSDHFRFWLVKIWHALF
                     FCVLPIYIFGFWPWLLGFIIMSLTAGFCLSIVFQLAHTVEDTSFPLAN"
     gene            complement(824..1705)
                     /locus_tag="PKV31_09945"
     CDS             complement(824..1705)
                     /locus_tag="PKV31_09945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB93223.1"
                     /translation="MRLFLFLPIVALLMTSCAKPLADFAVNSSEKKVTAPIHFDNKSK
                     KATTFHWDFGDGKTSADPSPTHIYYHSGRHEVTLKASDGKKSTIKKMEINIEPPTHCE
                     VLIETEFGNMLVQLSDATPLHRDNFIKLAEQGFYDGLAFHRVIRNFMIQGGDPNSKDE
                     NSQALGSGGPGYTIPAEFVDTLFHLRGAIAAARTGGPMNPEKKSSGSQFYIVQGDQVD
                     DAYLNAREASGKVRYPTSVKEAYKAMGGTPHLDGEYTVFGKVIQGLDVIDKIASQQTG
                     PGDRPVKPIRMKVIVIK"
     gene            1773..>2148
                     /locus_tag="PKV31_09950"
     CDS             1773..>2148
                     /locus_tag="PKV31_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNB93224.1"
                     /translation="MFRSLIFSLLFFVFSLPVFAGEGMWLPILLSQLNEKEMQSKGMK
                     MTAADIYSVNQGSLKDAIVQFGGGCTGEVISTTGLLLTNHHCGYGQIQRHSTLENNYV
                     KNGFWAKSKSQELSNPGLTVTFI"
BASE COUNT          625 a          461 c          460 g          602 t
ORIGIN      
        1 tcattagcca aaggaaagga agtatcctct acggtatggg ccaactggaa aacaatgctc
       61 aggcaaaatc cagcggtgag gctcataatt ataaatccaa gcaaccatgg ccagaagccg
      121 aaaatatata ttggcaagac acaaaagaac aaggcatgcc agatcttgac cagccaaaat
      181 ctgaaatgat cactcatttt catatttgta atcgggaatt cgcctatttt cattttgaaa
      241 tactttttat aatctgctgc aaagacccaa tacaaataca tgaccatata caaggcagga
      301 aaatagatat gctgatactt gtggaatggc aatcgggtct ggcttggagc aagtctcatc
      361 aatttgccca cctcgatatc gtcatccaga tcatcgacat tggtataggt atgatggatc
      421 acattatgct tgatattcca gaagaaatgg ctggcgccca acatactcat ggtgtaactg
      481 gcaagacgat tgctactctg atgggtagaa aaactaccat ggctaccatc atgcatgata
      541 ttgaaaccta ttgttgctac caaaaggccc aacaggatac attcggaata ggcaatagcc
      601 caatggggct ggaatataaa caaatggcca acggtaagtg cgaataaagt gagtaaaatt
      661 gctgctttcc agtaaagatt ggcattgccg gtctgtcgaa ttttgttttc ttcaaaatac
      721 aggctaacac ggcgtcttaa ttcactgtgg agggatggtc tgacttgagg aaatcttgga
      781 aatgctttgg tcgaagccat tatgctatta tttatttgat tgattactta attacgatga
      841 ctttcatacg aattggttta acaggtcggt caccggggcc tgtttgttgg gatgcgatct
      901 tatcaattac atcaagtccc tgaattacct tgccgaaaac ggtgtattcc ccgtccaaat
      961 gaggtgtgcc ccccatggcc ttgtaagctt ccttgacgga agtagggtac ctgaccttgc
     1021 cggaggcctc tctagcgttc aggtaggcat catcgacctg atcaccctga acaatgtaga
     1081 attgagatcc actggatttt ttctccggat tcatagggcc acctgtcctt gctgccgcaa
     1141 tagcgcctct caggtgaaac agagtatcca cgaattcagc gggtattgtg tatcccggac
     1201 cgccactacc taatgcctgg ctgttttcat ccttggaatt agggtctcca ccctggatca
     1261 taaaattcct gattaccctg tgaaaggcca atccgtcata aaatccctgt tcggccaatt
     1321 tgatgaaatt atcacggtga aggggtgtcg cgtcagacag ctggacaagc atattgccaa
     1381 attctgtctc aataagtacc tcgcaatgcg taggcggttc gatattaatc tccatctttt
     1441 taattgttga tttttttcca tcagaagctt tcagtgtaac ttcatgccgg ccactgtgat
     1501 aatagatatg tgtaggagag ggatctgccg aagtcttgcc atcaccgaaa tcccaatgaa
     1561 aagtagtggc tttttttgat ttattatcaa aatgaatagg agcagtcact ttcttttcgg
     1621 aagaattgac tgcgaagtct gccaaaggct ttgcacagct tgtcatcagc aatgccacaa
     1681 taggcaaaaa aagaaatagt cgcatttatt ccgtaaattt tgcgctaaaa tacagtcatt
     1741 ttactaagtt tgagcaaaat aaaatattaa atatgttcag gtctttaatt ttctcactat
     1801 tattttttgt cttcagcctt ccggtcttcg cgggagaagg gatgtggctg cctattttac
     1861 tatctcagct caatgaaaag gaaatgcaat caaagggcat gaagatgact gctgccgata
     1921 tatacagtgt caaccaagga agcctcaagg acgccatcgt tcaatttggc ggggggtgca
     1981 ccggagaagt catttccact accggcttgt tattgaccaa tcatcattgc ggttatggcc
     2041 agatacaaag gcattcaacc cttgaaaaca actatgtcaa aaatggattc tgggcaaaat
     2101 caaagtcaca ggagctctcc aatcccggtc ttaccgttac attcatcg
//
LOCUS       DAOQMC010000329         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_34048_length_2143_cov_4.035920, whole genome shotgun sequence.
ACCESSION   DAOQMC010000329 DAOQMC010000000
VERSION     DAOQMC010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34048_length_2143_cov_4.035920"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PKV31_09955"
     CDS             <1..1572
                     /locus_tag="PKV31_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB93225.1"
                     /translation="AIEQNPTMLNLRVSYASLLNTLNKPKEAEAQYNYVMKNIPANPT
                     VILLTSRALEKAGKPELAIQVLEKGRDLVPNANYFTYDLSQLYLKTGQQEKMLAIAVD
                     QLIADPSYYSLFTTSIQRSFQSDSEYDNLLKAVFNRAQQYPDNSQLIELMSWIYMQKR
                     DFANAMRQLKALDIRTGNDYTRVYSLARTAFNEKQYDVAVSGFDYILEKGRANPYFYE
                     AARFRLQALVAKSVVNTIISPSEQEVIDKDYTDYINTYSETGRAVYLSYDYAEFLMQY
                     RDNLPKAIEILQTIVDNGTYSPPVIAEVKIRLADYLLIEGKRWDASLLYSQVDKDFKE
                     GPLGQEARYKNALLSYYVGDFEWSQAQFDALKTATSRLISNDAIDRSVFIMDNMGMDS
                     TDLAMKKYAATELLIYQNKLSEALVKLDSILIDFPGHALDDDVLYAKAHIFLKMKDYN
                     KAIDCYEKVFTKYKDEIRADNSIYELAQLYDYTLNDKEKAKALYERLFNEYTGSVFAF
                     DARQRFRVLRGDVDQ"
     gene            complement(1652..>2143)
                     /locus_tag="PKV31_09960"
     CDS             complement(1652..>2143)
                     /locus_tag="PKV31_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB93226.1"
                     /translation="IDLSQVGSGNDIVGMGNDYRDVFTALLQDWLGASDRVLEDTYFG
                     DYVTTKAPILESQYVVSPECYIDTYLSSSIFNRPAQLSLYPNPARNVFSFQINPGGQG
                     VAFITLTDTSGRTIKQWKMNYVNGENNLTFGITNVEAGIYMVQMTLSESRRRYTGKLV
                     VID"
BASE COUNT          626 a          449 c          486 g          582 t
ORIGIN      
        1 gccattgagc agaaccccac catgctcaac ctccgtgtgt cctatgcttc cctgctcaat
       61 accctcaaca agcccaagga agccgaagca caatataatt acgtaatgaa gaatataccc
      121 gctaatccaa cagtaatttt gcttacatcc agagccctgg aaaaggccgg taagccggaa
      181 ttggcgatcc aggtgttgga aaaaggccgg gatcttgtcc ccaatgctaa ttattttacc
      241 tatgacctga gccaactgta tttgaagaca ggacaacagg aaaaaatgct tgccatcgca
      301 gtagatcagt tgatagcaga cccaagttat tacagcctgt ttacaacttc catccagcga
      361 agcttccaga gtgattcgga gtatgacaac ctgcttaagg cggtttttaa ccgagcgcag
      421 cagtacccgg acaattcaca gttgatagaa ttgatgtcgt ggatttatat gcaaaagcgg
      481 gatttcgcta atgcgatgcg acagctcaag gcgttggata tacgtaccgg caatgactac
      541 acccgggtat actcattggc gagaacggct ttcaacgaga aacaatacga tgtcgccgtg
      601 tcgggcttcg attatatttt ggaaaaaggc agggccaatc cctattttta tgaagcggcg
      661 cgtttccgct tgcaagcttt ggtagctaaa agtgtggtca acaccattat ttctccgagc
      721 gaacaggaag tgatagataa ggattatacc gactatatca atacttattc cgagaccggc
      781 agggctgtgt acctatcata tgattatgct gaatttttga tgcaatacag ggacaatctt
      841 cccaaagcaa ttgaaatttt acaaaccatc gttgataatg gcacatattc ccctcctgtg
      901 attgcagagg taaaaatccg actggctgat taccttttga tcgaaggaaa gcgctgggat
      961 gcttctttac tctatagtca ggtggataaa gacttcaagg aaggtccctt aggccaggag
     1021 gcaaggtata aaaatgctct tctttcttat tatgtgggtg attttgaatg gtctcaggcg
     1081 cagtttgatg ctcttaaaac tgctacatcg aggttaatat ccaatgacgc catcgacagg
     1141 agtgtattta tcatggataa tatgggcatg gattcgaccg atttggcgat gaagaaatat
     1201 gctgccaccg aattgctgat ttatcagaat aaattatcag aggcattggt taaactggat
     1261 tccatattga tagattttcc cggtcacgca cttgatgatg atgtgttgta cgccaaggcg
     1321 cacatatttc tgaaaatgaa agattataac aaggccattg attgctacga aaaagtattt
     1381 actaaatata aggatgaaat tcgggcagac aattctattt atgaattagc tcaattgtat
     1441 gattataccc tcaatgataa ggaaaaggcg aaagcgttgt atgaacgatt gttcaatgaa
     1501 tacaccggga gcgtttttgc ctttgatgca agacaacgat ttcgggtttt gcgaggagat
     1561 gtagatcaat aaagcctgtc cctggaaaaa gaatttcctc agggacaggc tctgctatgc
     1621 tacggtcggc tgaaaacagt tacaggaagg gtcaatctat caccacgagc ttgcccgtat
     1681 accttcgtct gctttcgctc agcgtcatct ggaccatata aatacctgcc tctacgttgg
     1741 taataccaaa agttagatta ttctcaccat tgacataatt cattttccat tgcttgattg
     1801 ttctgccgga agtgtccgtt agtgtgatga atgcaacacc ttgtcctccc ggatttatct
     1861 ggaaggaaaa tacattacgt gccggattgg ggtacaggct gagttgggca ggacgattga
     1921 atattgatga ggaaaggtat gtgtcgatat aacattccgg gctcactaca tactgacttt
     1981 caaggatagg agctttggtc gtgacgtaat ctccaaaata ggtatcttcg agaacccggt
     2041 cactggcacc aagccaatcc tgcaaaagag cagtgaatac gtcacggtaa tcattgccca
     2101 tgcctacgat atcattgcct gaaccaactt gcgaaagatc gat
//
LOCUS       DAOQMC010000330         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_34780_length_2117_cov_4.510669, whole genome shotgun sequence.
ACCESSION   DAOQMC010000330 DAOQMC010000000
VERSION     DAOQMC010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34780_length_2117_cov_4.510669"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /gene="typA"
                     /locus_tag="PKV31_09965"
     CDS             complement(<1..1599)
                     /gene="typA"
                     /locus_tag="PKV31_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNB93227.1"
                     /translation="MAQEIRNIAIIAHVDHGKTTTVDKILHQTNLFRDNQNPGELILD
                     NNDLERERGITIVSKNVAVNYKGVKINIIDTPGHADFGGEVERVLKMADGVLLLVDAF
                     EGPMPQTRFVTSKALSLGLKPIVVINKVDKENCRPDEIHEMIFDLFFALDATEEQLDF
                     PVVYGSSKHGWMSNDWKKPTTDFTALLDAIIEHIPSAPHNEGTLQMQVTSLDYSSFVG
                     RIAIGRVSRGSIKENQPIALMKRDGTVVRGRVKELHTFEGLGRKKTNEVGCGDICAVV
                     GLDGFDIGDTISDVENQEALPGIQIDEPTMSMLFTINNSPFFGKEGKYVTSRHIRERL
                     MKETEKNLALRVEDTESPDAMMVFGRGILHLSVLIETMRREGYELQVGQPRVIIKEID
                     GRKHEPIETLYVDVPAELSGKVIELATQRKAELTVMEPKGDLQHLEFNIPSRGIIGLR
                     NQVLTATAGQAVMNHRFKSFEPWVGDIPGRSMGVLVSMETGPCTAYALDKLQDRGTFF
                     VDPGEEIYEGQVVGEHIRENDLVIN"
BASE COUNT          547 a          483 c          464 g          623 t
ORIGIN      
        1 attgataaca aggtcatttt cgcggatatg ctctcccaca acctgacctt cgtagatttc
       61 ttctcccgga tctacgaaga aggtacctct gtcctgtagc ttgtccaatg catatgccgt
      121 acaaggtcct gtttccatag aaaccagaac acccatactc ctgcccggga tgtcgccaac
      181 ccatggttcg aatgacttga acctgtgatt catcactgcc tgaccggctg ttgcagtcag
      241 gacctgattg cgcagaccga taattccacg tgaaggtata ttgaattcaa gatgttgaag
      301 atcacctttt ggctccatta cggtcaactc tgcctttcgt tgtgtcgcca gttctatcac
      361 ttttccggac agttctgcag gcacatctac atagagggtc tcgataggtt catgcttcct
      421 gccatcgatc tccttgatga ttacacgtgg ctggcctact tgtagttcat atccttcacg
      481 acgcatggtc tcgatcagta cagagaggtg caggatacca cggccgaata ccatcattgc
      541 atccggtgat tctgtgtcct ccacacgaag ggctagattt ttttcggttt ccttcatcag
      601 tcgttcgcgg atatgtcggg aagtgacata tttgccttcc ttgccgaaga aggggctgtt
      661 gttaatggtg aagagcatac tcatcgtagg ctcgtcaatc tgaatacctg gtaatgcctc
      721 ctgattctcc acatcactga tggtatctcc tatgtcaaag ccatccaggc cgacaacggc
      781 acagatgtca ccacaaccta cctcgttagt cttttttctt ccaagacctt cgaatgtatg
      841 taattccttc acacggcccc ttacaacagt gccgtctctt ttcatcaatg caatcggctg
      901 attttccttt atgctgcccc tgctcaccct gccgatggcg atacgtccta cgaaggagga
      961 atagtccagt gaggtaactt gcatctgtaa agtgccctca ttgtgagggg ctgaaggtat
     1021 atgctcgatg atggcatcca gcaatgcggt gaagtcggta gtaggttttt tccagtcatt
     1081 actcatccag ccatgctttg acgaaccata gacaacagga aagtcaagtt gttcttccgt
     1141 tgcatcgagg gcgaagaaca ggtcgaaaat catttcgtgg atctcatccg gtctgcaatt
     1201 ttccttgtcc actttgttga tcaccacgat aggctttaga cccagggaaa gagcctttga
     1261 ggtgacaaat cttgtctgag gcatcggacc ttcgaaggca tcaaccagca acagaacacc
     1321 gtcggccatc ttaagaacac gctcgacttc acctccgaaa tctgcgtgac cgggagtgtc
     1381 aatgatgttg atctttactc ccttgtaatt gacggcgaca ttttttgata cgatggtgat
     1441 gccacgttcc ctttccaggt cgttgttgtc cagaatcaat tcacccggat tctgattgtc
     1501 gcgaaacagg ttggtctgat gaagaatctt atcgacagtg gttgtcttac cgtggtctac
     1561 gtgggcgatg atagcgatat ttctgatttc ctgagccatt caatattttg ttttgaaaaa
     1621 agaggcgcaa aggtacgttt ttttgtcttg aaatatatac ataaaatgca aatattcaat
     1681 tcattatcaa attgttcggc ttttttcttt tcaggaaaaa tattatatgt taaaccgggt
     1741 cgggcaaatg tataagattg tgtaaccggt caggcgcatt gccgataaat agtgaacaat
     1801 aattcatagt aatgattcaa aagcggaaca tcaacaaaat ccaccgggaa cccgaacaga
     1861 ttcagaaatg aatatatcat cagaaacgct tattgctttg attagaggtt tgcaaggatt
     1921 ttgtcggctg aatctaattg gtcaaaaatc cctataaatt ggttgcctct gatagcaatc
     1981 atgtcaaatt cagtgcatct cggccatata ggtgattggt tttaatggag aagcatcaat
     2041 tttcaattta cgaattgatt aaaaaaaggc aaaatggttg aagttttgag gattgctgag
     2101 atattgaggc aggatac
//
LOCUS       DAOQMC010000331         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_34812_length_2116_cov_3.868510, whole genome shotgun sequence.
ACCESSION   DAOQMC010000331 DAOQMC010000000
VERSION     DAOQMC010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34812_length_2116_cov_3.868510"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKV31_09970"
     CDS             complement(<1..638)
                     /locus_tag="PKV31_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNB93228.1"
                     /translation="MTDYYQILGLPAGASATEVKKAYHKLALKYHPDKNPNNTNAEYI
                     FKQITEAYNCLSNPADKLLYDMQFSVQQQSLAHVYRDDKKYQEAKKQQVTPTAMLTIF
                     QNLRREVARVDKSKIDPGRYFVSINNLLSIANIDYFLSVGDIATNRDIVFEAMACCKP
                     LPYFFVNRLVPRLVKLAGADNETIVKIYGFDRRHRMREKWIRYLPLILFLMY"
     gene            complement(921..>2116)
                     /locus_tag="PKV31_09975"
     CDS             complement(921..>2116)
                     /locus_tag="PKV31_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB93229.1"
                     /translation="TDPQVFPFVNDSMNYLSPCFGRDSNLLFFAAHDPQLNSGFDIYY
                     SQRLNGVWQEPIRLPGIVNSEYDEKYITFDRDTLYFSSNRPSGMGGLDIYKTYVARGS
                     WMPPVNMKAPVNSPYDDFSMFFEKDIGGDDKVLARGFLTSNRPGGNGSDDIYRFTKVR
                     LKETKPVIDTPDIDDKSILVRLNVFVRGHNIVDGKLAGGTTELSSATVKINSGGTDTT
                     IQADKYGKSSTLIRIVDQMVITASKEGYLTSSKTIDRQVIRIDSTKRIQEFNMVMNLY
                     PVLYDQEIIIKDIYYDLDKYDIRKDAIGSLDELLSILRLNPSFKIRINSHTDCRGSEA
                     YNKTLSARRAKSVVDYLTANGINRNRLSSRGFGEMMPLAKCPCEKCTEEEHQLNRRTT
                     FQLVR"
BASE COUNT          598 a          444 c          436 g          638 t
ORIGIN      
        1 atatacatta aaaataagat caatggtaag tagcgtatcc atttttcgcg catccggtgt
       61 cgcctatcga aaccataaat cttcactatt gtttcattgt ctgctcctgc aagcttaacc
      121 aatcgtggta caagcctgtt gacaaaaaaa tagggaagcg gcttacaaca tgccattgcc
      181 tcaaaaacaa tgtccctgtt cgttgcaata tctcccactg aaagaaaata atcgatattg
      241 gcaatagaca agaggttgtt gatggataca aaataccggc cggggtctat cttactttta
      301 tctacgcggg cgacttccct tctcagattc tgaaagatgg tgagcatggc tgttggagta
      361 acctgttgct ttttcgcttc ctggtatttt ttatcatccc tgtatacgtg tgcaagactc
      421 tgttgttgaa cagagaattg catatcatag agcaatttat ctgcaggatt ggaaagacaa
      481 ttgtacgctt cggttatttg cttgaagatg tactcagcgt tggtattgtt cgggttttta
      541 tccggatggt atttgagggc aagcttgtga taagcttttt taacttctgt agccgatgct
      601 ccggcaggca gacctaatat ttggtagtaa tcggtcatcc tgctacatct attacattat
      661 aaatcatgac aatggtaacg gagaatcctc ttaattcaat aaaattactg ccttgcttca
      721 ctgtgttaca atgtttggaa tgaaggtaag gaaaaaatac ggtagacaaa atacggatat
      781 tatgtacaat tactttaaat atgtataata ctgatttata atattttaaa tatatcataa
      841 tgatatagta acctgccgag gtttccaagg atcattacaa agaaaccttc gcaggtccat
      901 agttcctcag ctgcctaaag ctatctgaca agctggaagg ttgtcctcct gttgagctga
      961 tgctcttctt cggtacactt ttcacaagga cattttgcca gcggcatcat ttcaccgaat
     1021 ccccgggagg agagcctgtt gcggttgatt ccattggcag tcagatagtc cacgaccgat
     1081 ttggcccgcc tggcagataa tgtcttgttg taagcttcac taccccgaca atcagtatgg
     1141 gaattgattc tgattttaaa cgaaggattc agtctcaaaa tagacaaaag ttcatccaga
     1201 gagccgattg catccttcct gatgtcgtac ttatccaggt cataataaat atccttgatt
     1261 atgatttcct gatcgtacaa aacaggataa agattcatca ccatgttaaa ttcctgaatc
     1321 cgtttcgtgc tgtcgatccg gatcacttgc cggtcaatgg ttttggaaga tgtgagatag
     1381 ccttccttag aggctgtgat gaccatctgg tcgactattc gaatcaaggt agaggacttt
     1441 ccatatttgt cggcctgaat ggttgtatct gttccgcctg aatttatctt aacggtcgct
     1501 gatgatagtt ccgtggtacc gccggctaat tttccatcca caatattgtg tccccggaca
     1561 aagacattca accttaccaa aatacttttg tcatcgatgt cgggtgtatc gataacaggc
     1621 ttggtttcct taagccttac ttttgtaaat cggtaaatat catcactgcc atttcctccg
     1681 ggtctattgg aagtaagaaa tccacgtgcc aataccttat catcaccgcc aatatctttt
     1741 tcaaaaaaca tcgaaaaatc atcataagga gaattgaccg gagctttcat attaaccgga
     1801 ggcatccagc ttcctcttgc cacataggtt ttgtatatat ccaggccacc cataccgctg
     1861 ggtcggttgg aggagaaata cagggtatcc cggtcgaagg tgatgtattt ttcatcgtat
     1921 tccgaattga cgatgcccgg aagacgaatc ggctcctgcc atacgccatt caaacgttgg
     1981 gaataataaa tatcaaatcc ggaattaagc tgtggatcat gggctgcaaa aaaaaggagg
     2041 ttactatcac ggccaaaaca aggactcaga taattcatac tgtcattgac aaatggaaat
     2101 acctgaggat ctgtcc
//
LOCUS       DAOQMC010000332         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_34960_length_2111_cov_5.449903, whole genome shotgun sequence.
ACCESSION   DAOQMC010000332 DAOQMC010000000
VERSION     DAOQMC010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34960_length_2111_cov_5.449903"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1911
                     /locus_tag="PKV31_09980"
     CDS             163..1911
                     /locus_tag="PKV31_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB93230.1"
                     /translation="MPSLDPFKSTNLFLRPGGLPTMAHSILCGKDAQSRGDKMRRKLL
                     YLVVLFAILLSACSGGGNQPAVVRIGWSGSPDTLNPGNAILAVSYTIFELVYDSMYNL
                     NLDGTFSLSLAESASVSDDGVTWTFKIRDGVKWHDGQPLTAEDVAWTYNLYKNTPEYP
                     YLSGYYTTYFDTIVAAENNEVILTLTEPIPNIESQLVFLYILPKHIWENVDKLEYENT
                     DMIGSGPFKMLEYSQNEFVRLGANKEYFEAPPKVDEVIFQTFENQDALVQAIKTGQVD
                     MITEMPNTAAQSLKEEKDIELVVGAPFSPGVTDIIFNQIDPENCPTEEGGICSGHPAL
                     RDRNVRLALAHATDKKRLIDVILLGLGTPGLTLIPDGLGVWYNSSLSDYEFDIAKANQ
                     ILDDAGYVDANGDGIRDMPDGSQPLTFRLNWPNDSTDAPRMAELLAEMWGQLGITLEP
                     QAVDPDALTAQCCPAFDFDIMLWGWASDPDPSALLYVYTTDSIPYGSSETGYSNPDYD
                     ALYTQQQLELDTNARKDIVWEMQKIVHDDVVYIIPYYAASVQAFRTDRFTGWITDQPK
                     VELPDVSSLMIIEPVK"
BASE COUNT          552 a          448 c          501 g          610 t
ORIGIN      
        1 caacctcatc ccaagtcagg tcgtcataat ggaaaaaagt tgtgttgcgc gtagtcattt
       61 tacctactcc tattatcatt tatttaattc caagcacaaa attccacttg tacaactaac
      121 atgcttttcc gataacaaaa tatctagtaa aattttgaat agatgccctc tttagaccca
      181 ttcaagtcaa caaacctttt cctgcgccct ggcggtttgc ccaccatggc gcattctatt
      241 ctttgcggaa aagatgctca atcacgagga gataagatga gaagaaaact tctgtacctg
      301 gttgtgttgt ttgcaatttt actttctgcc tgtagtgggg gaggtaatca acctgccgtt
      361 gtgcgtattg gatggtcagg cagtccggat acgctaaatc caggtaatgc gatcctggcg
      421 gtgtcatata caatctttga actggtgtat gactctatgt ataaccttaa tctggatggc
      481 acgtttagct tgagcttggc ggagagcgcg tcggtttcag atgatggtgt cacctggaca
      541 tttaagatcc gtgacggggt gaaatggcac gatggacagc ctctgaccgc tgaagatgtt
      601 gcctggacgt acaatctgta taagaataca cctgaatatc cgtacttgag cgggtactat
      661 actacatatt ttgacaccat tgttgctgcg gaaaacaatg aggtcatcct gactcttacc
      721 gagccaatcc cgaatattga aagtcaattg gtgtttttgt atattcttcc aaagcatatc
      781 tgggaaaatg tggataagct tgaatatgaa aataccgata tgatcggttc cgggccgttc
      841 aagatgctgg aatattcaca aaatgaattt gtccgtcttg gggcaaacaa ggaatatttt
      901 gaagcgcctc ccaaagttga tgaagtgatc tttcaaacgt ttgaaaatca ggatgcattg
      961 gtgcaggcga tcaaaacggg ccaggtggat atgatcaccg agatgcctaa taccgcagct
     1021 caatcgttga aggaagagaa ggatatcgaa ctggttgttg gtgcgccctt ctcacctggt
     1081 gtaacggata tcatattcaa tcagatcgat cctgagaatt gcccaactga agaaggcggc
     1141 atttgttccg gccatccggc tttgcgagac aggaatgtgc gtctggcgtt ggctcatgca
     1201 accgataaaa aacgcctgat tgatgttatt ttgctggggc ttggaactcc cggcctgacc
     1261 ttaatcccgg atggattggg ggtttggtat aacagttcat tatcggacta tgaatttgac
     1321 attgccaagg ctaatcagat ccttgatgat gccgggtatg tggacgcaaa tggtgatgga
     1381 attcgtgata tgccagatgg ctcacagcca ttaaccttcc gcttgaactg gccaaacgac
     1441 agtaccgatg cccctcgtat ggcagagttg ctggcagaga tgtgggggca gctggggatc
     1501 acgcttgaac ctcaggctgt tgatccggat gcactgaccg cccagtgttg tcccgccttt
     1561 gatttcgata taatgctatg gggctgggct tccgaccctg acccaagcgc cttattgtat
     1621 gtgtatacaa cagattcaat tccttatggt tcgagtgaga ccggttactc caatcctgac
     1681 tatgacgcgc tttacaccca acagcaactg gaattggata ccaatgctcg caaggatatt
     1741 gtttgggaaa tgcaaaagat cgtccatgat gatgttgttt acatcattcc ttattatgcc
     1801 gcaagcgttc aggcttttcg aacggatcga ttcacaggct ggatcactga tcaaccaaag
     1861 gttgaattgc cagatgtaag ttcactgatg attatcgagc cggtcaagta actttttgaa
     1921 gttcttattc ccgcaggtca ctgagttttg acctgcggga atatttagtt gcaacagagt
     1981 taagatgtaa aataaatatc acgagagtgt accttggacc gtattcgcgc aattcttcaa
     2041 aaagcaatct gggctgtttt tacgatcata tttgtcatcg ttcttaattt ctttttgttt
     2101 cgtgttctac c
//
LOCUS       DAOQMC010000333         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_35361_length_2099_cov_3.618885, whole genome shotgun sequence.
ACCESSION   DAOQMC010000333 DAOQMC010000000
VERSION     DAOQMC010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35361_length_2099_cov_3.618885"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKV31_09985"
     CDS             complement(<1..226)
                     /locus_tag="PKV31_09985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB93231.1"
                     /translation="MQQVTISAVIPVFNAQQTLRRAVDSLLNQTWLPVEIILVDNNST
                     DGSPAIMEEYRRRYPDLISIIHQPVPGANAA"
     gene            complement(228..2009)
                     /locus_tag="PKV31_09990"
     CDS             complement(228..2009)
                     /locus_tag="PKV31_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB93232.1"
                     /translation="MTKETTLTGSGKYLGYFQSLVGSRIYLSLALSVVITLFDSVGIG
                     LLVAILQFMANSENGDSSLSLNAYYKQVFGQDATFLMLILIAIFIFLLKGLLTFTLYS
                     VQARMTTTIISKTRHNILDSIVNLNFASFTKTDLGTIQNISTSEVGRLNIALNSYLKT
                     GQYLIMSVVYLALAIVFDPVFSFLIIFTALAVLYLYNFIIKYFKKLSRQILSSRNQYS
                     SFLTQLLTNYKYLKTTDTIDKYSRKVKDQIDHTESLNYRFLKINAITDSAREPIILVM
                     ISLAIILLFHVKGGIGISMIFTMLLFYRTLNNVLLIQANWQGFHQNSGSVDRIISIQE
                     EFSKDMEAIGTDSAFHIAGRISLEKVDLNLSDKQILSDISLTLEKNSTTALVGPSGAG
                     KTTLASVITGLIQPNSGNISINNRLLESSDLKGYRRKIGFVTQDPVIFNDTLFNNITL
                     WEEKNSQSVKRFSEICRMTHLEDFVQAQLDKEDMQLGDFGITLSGGQKQRISIARELY
                     RDVELLVFDEATSSLDAITEQIIRENIQSIRGKVTIILIAHRLSTIRFADRIYFIEGG
                     RITASGTYDELMQTSPDFLKMVNLQSG"
BASE COUNT          615 a          418 c          439 g          627 t
ORIGIN      
        1 gggcggcatt ggctcccggc acaggctggt gaataatcga aatcaaatcg ggataccgtc
       61 gtcgatactc ctccatgata gccggactgc cgtcggtgct gttgttgtcc accaggatga
      121 tttctacagg cagccaggtc tgattgagca agctatcaac tgcccgtcgc aatgtttgct
      181 gagcgttgaa aacgggaatt acggcactga tggtaacttg ctgcatatta gccggattgc
      241 aggttgacca ttttaaggaa gtcgggtgat gtttgcatca gttcgtcgta ggtgccggaa
      301 gcagtgatac gacctccttc gatgaaataa atccgatcgg caaatcggat ggtagacagc
      361 cggtgggcga tgaggatgat ggttaccttg ccgcggatgg actggatatt ttcccggata
      421 atctgctcgg tgatggcgtc caaagatgag gtagcttcat caaagacaag caattccacg
      481 tcacggtaca attcccttgc aatactgatt cgttgtttct ggccaccgga tagggtgatg
      541 ccgaagtcgc ccagctgcat atcttcttta tcaagttgag cctgaacaaa gtcctccaga
      601 tgcgtcatcc tgcatatttc actaaatctt ttgacagatt gactgttttt ttcttcccaa
      661 agagtgatat tgttaaacaa agtatcattg aatataacgg gatcctgagt tacaaaccca
      721 attttccggc gatatccttt caggtcagat gattccagaa gtcgattatt tatggatatg
      781 tttccggaat tgggttgaat taatccggtt atgacagacg ccaaagttgt ctttcctgcg
      841 ccacttggcc ctaccaatgc agttgtagaa tttttctcaa gcgtcaggct gatgtcgcta
      901 agaatttgtt tatctgaaag attaagatct accttttcca atgaaatccg accggctata
      961 tgaaaggcac tgtctgttcc aattgcttcc atatctttcg aaaattcctc ctgaatagaa
     1021 ataattctgt caacgcttcc tgaattctga tgaaatccct gccaattggc ctgaatcaat
     1081 aggacattat taagcgtacg gtaaaataag agcatggtga agatcatgct aataccgatg
     1141 cctcctttta cgtggaacaa taatataatg gccaggctta tcatgaccag aataataggt
     1201 tctctggcac tatcagtgat ggcattgatc ttaaggaatc tgtaatttag cgattctgta
     1261 tggtctatct gatctttgac cttcctactg tatttatcaa tagtgtctgt agttttcaga
     1321 tatttataat tcgtcagaag ctgagttaga aaactactat attgatttct ggagctcaga
     1381 atctgtctgg atagcttttt gaaatatttt attataaaat tatacagata caatactgcc
     1441 aaagctgtga aaataatcaa aaaagaaaaa acagggtcaa aaactatcgc aagtgccaga
     1501 tatactacgg acattatcaa atattgtccg gtcttcaaat agctgtttaa agcgatgttg
     1561 agccttccta cttcggaggt tgagatattt tgtatcgtcc ctaagtcggt ttttgtaaat
     1621 gatgcaaaat tgagattgac aatactatcc agtatattat gtctggtttt gctgatgatg
     1681 gtggtagtca tcctcgcctg aaccgaatat agggtgaaag tgagtaaacc tttcaataaa
     1741 aaaataaaaa tagcaatcaa aattaacatc aaaaatgtag catcttgccc gaaaacctgc
     1801 ttgtaataag catttaagct gagagaagaa tctccattct cactatttgc cataaattgg
     1861 agtatagcga ccaacaatcc aatccctaca ctgtcaaaga gtgtaatgac aacagataaa
     1921 gccagtgata aataaatacg gctacctaca agtgactgaa aataaccgag atattttccg
     1981 gagccggtca gtgtggtttc cttcgtcaat cagtatactt gaaattataa atgttgctca
     2041 aaataatcaa taactttcct gatcaggtgt actctgtctt tgcccagcac attgtgctt
//
LOCUS       DAOQMC010000334         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_35700_length_2088_cov_4.929661, whole genome shotgun sequence.
ACCESSION   DAOQMC010000334 DAOQMC010000000
VERSION     DAOQMC010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35700_length_2088_cov_4.929661"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKV31_09995"
     CDS             <1..393
                     /locus_tag="PKV31_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93233.1"
                     /translation="IPFSAEYNGMESWANFPHEQNRLFKLLDKTRALGVVFLSSHAGY
                     SEISKLKRDGYYPIYDYTCAGLNTQKYFATANANRIEGPIMEPNFGLLTFDWAPGKVT
                     MTMECIDANNNSRFEYIVKLTHLWPDSE"
     gene            complement(470..1051)
                     /locus_tag="PKV31_10000"
     CDS             complement(470..1051)
                     /locus_tag="PKV31_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNB93234.1"
                     /translation="MGTMKHLAIFASGGGSNAQAIMKHFRNHPTIQIALVLYNKKDAG
                     VKNHAAHFGIPARFWSGKAMADQERTESLLGEYRITGIILAGYLSLIPEYLINQFPDK
                     ILNIHPALLPSFGGKGMYSHFVHEAVSKAGHIETGITIHLVNKEYDKGRILFQKAISI
                     RPHEDSGVIARQVLKIEHQHYAHVIEDYFESLH"
     gene            complement(1058..1984)
                     /locus_tag="PKV31_10005"
     CDS             complement(1058..1984)
                     /locus_tag="PKV31_10005"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNB93235.1"
                     /translation="MKRMIMRCTFLIALLLIFFSFKGFSQEFAFTVKVNTPTLQIADP
                     GIFKNFEKSILEFLNNQRFTDMDLRDEEKIKVTLNFNLVKEQSEKDFVFSLAILAVRP
                     VYGSSYESVIFQYVDDKVVFSYEPGNALIFNPNSYNDNLTSILGYYAYIILGLDRDSF
                     AEKGGDQMFVNAQTVIGSLPTGLSNGDEYGWGANKAGLNKSRSSFVENLLSPKLNDFR
                     IGFYEYHRQGLDLASTNIDEARKNIINAMKSIEKSYDTYTNSIEINSFGTVKGNELVE
                     IFKPASKAEKQDIYKIMSKLDPAGLQKYIDLR"
BASE COUNT          605 a          436 c          428 g          619 t
ORIGIN      
        1 ataccgttca gtgcagaata caatggcatg gaaagttggg caaatttccc gcatgaacaa
       61 aaccggttgt ttaagctcct tgataagaca agggctcttg gtgtagtctt cttatccagt
      121 catgccggtt actctgaaat cagtaaatta aaaagggacg gctactaccc catctacgac
      181 tatacctgtg ccgggctcaa cacgcaaaag tattttgcta ctgccaatgc taacaggatt
      241 gaggggccga taatggagcc caactttggt ctgttgacat tcgactgggc tccaggcaag
      301 gtcactatga ccatggagtg cattgatgcc aacaataaca gtcggtttga atatatagtt
      361 aaactcacac atctttggcc ggacagtgag taatctgaag ttactaaatg catctgccga
      421 atagaactaa gttatcgaca ggtgattcgc gactctggta aaacaaattt tagtggaggg
      481 attcgaaata atcctcaatg acgtgggcat agtgttgatg ctcgattttg agaacttgcc
      541 tggcaatgac cccggaatct tcgtgcggtc ttatggagat tgccttttga aacaagattc
      601 tgcccttgtc gtattcctta ttgaccaaat gaatggttat accggtctca atgtgaccgg
      661 ctttacttac agcttcatga acaaagtggc tatacatacc cttaccacca aaggaaggta
      721 gtagtgccgg gtgaatatta agaattttgt ccggaaattg attgataaga tattcaggaa
      781 tcaaggaaag atatccggcc aaaatgatac ctgtaatccg gtattcacca agtaggcttt
      841 cagtcctttc ctggtcagcc attgcttttc cggaccaaaa acgagccgga ataccgaaat
      901 gggccgcatg gttttttacc ccggcgtcct ttttgttata aagaaccaat gcaatttgaa
      961 tggttgggtg attccggaag tgcttcatga tggcttgagc attgctgccg cctccggaag
     1021 caaagatggc gaggtgcttc atcgtaccca tatcatttca acgaagatca atatacttct
     1081 gaagacctgc cggatctaac ttagacataa ttttgtaaat atcttgcttt tcagcttttg
     1141 atgcaggttt aaaaatctcc accagttcat tcccttttac ggtaccaaat gagtttattt
     1201 cgattgagtt ggtatatgta tcataagatt tctcaatact tttcatggca ttgatgatat
     1261 tcttacgagc ttcatctata ttggtcgaag ccagatcaag cccttgtctg tgatattcat
     1321 aaaatccaat cctgaaatca ttgagtttgg gactcagcaa attttccaca aaagaagaac
     1381 gcgacttatt caatccggct ttatttgctc cccatccata ttcatccccg tttgacagcc
     1441 cggtaggtaa tgagcctatc acggtctggg cgttgacaaa catttgatcg ccgccttttt
     1501 ccgcaaagga atcccggtcc aggccgagaa taatatatgc ataataacca agaattgaag
     1561 tcaggttgtc attgtacgaa ttcggattaa aaatcaatgc attacccggt tcgtaagaaa
     1621 acacaacttt gtcatcaacg tattggaaaa tgacggattc gtaactgctg ccatatacag
     1681 ggcgaacagc gagaatggca aggctgaaaa caaagtcttt ttcagactgc tcctttacca
     1741 gattaaaatt gagggttacc ttgatttttt cctcatcacg caaatccata tccgtgaacc
     1801 gttggttgtt gagaaattcc aaaattgact tttcaaagtt cttgaaaatc ccgggatcgg
     1861 ctatctgcaa ggtgggtgta ttgaccttaa ccgtaaaagc gaattcctga ctgaatcctt
     1921 taaaactaaa gaatatcagc aataaagcta ttaaaaaagt acatctcatg atcatacgct
     1981 tcataacgat ttttttaata atttgattgt ttcatcgata atgattgtgg caatttctgt
     2041 tttaggcaac aattctgttt tccgaattat tccgctctct ccaaggat
//
LOCUS       DAOQMC010000335         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_35943_length_2081_cov_4.284304, whole genome shotgun sequence.
ACCESSION   DAOQMC010000335 DAOQMC010000000
VERSION     DAOQMC010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35943_length_2081_cov_4.284304"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKV31_10010"
     CDS             complement(<1..250)
                     /locus_tag="PKV31_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93236.1"
                     /translation="MRYKPWGEVRYTWKNSDPAVIPSAYALTRYTYTSQYSYMDDPST
                     SATEGFGLMFYNARWYDPALGRMAQADSVVPAGVQGYDR"
     gene            complement(428..769)
                     /locus_tag="PKV31_10015"
     CDS             complement(428..769)
                     /locus_tag="PKV31_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93237.1"
                     /translation="MKAIAIVSSVLYVLIIFFVSSNSLDLRKKSGWLYGMVSFLCGFA
                     LVYGLNGKVIDSAIVGIGFVFFILFNAMNERRHKREYERDTKTLIKEYGENKTFFGRL
                     VKKISVFLRKD"
     gene            complement(996..1175)
                     /locus_tag="PKV31_10020"
     CDS             complement(996..1175)
                     /locus_tag="PKV31_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93238.1"
                     /translation="MRYKPWGEVRYTWKASQTTTPAYALTRFTFTGQYSHIIPVGGMD
                     DPSTSGVTEGFGLMF"
     gene            1210..1494
                     /locus_tag="PKV31_10025"
     CDS             1210..1494
                     /locus_tag="PKV31_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93239.1"
                     /translation="MEVEPKWSPRKYSTLIYAKAHDQTVAEDYFQAMQRVEQRLNAST
                     VLSARFVPVEKNIKDVKVQNVFQLIESLALPDLCQEERLEIVGALKMALD"
     gene            complement(1583..>2081)
                     /locus_tag="PKV31_10030"
     CDS             complement(1583..>2081)
                     /locus_tag="PKV31_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93240.1"
                     /translation="SVYSFDGQVTDGRLMFSVEVISSECNNAGEPIKINMFFQNLTTG
                     PILITSGFNIINGGLGAGGNLTAIIFDSHKNRIYTLADRNLSSDTFYVPLNEYSTIFQ
                     NASKKFATDYYFPKKIYRNKTDEIRSTPTSGQYLIRFVYSDYQRENNNWEGVVSSNLI
                     TVCIK"
BASE COUNT          594 a          466 c          426 g          595 t
ORIGIN      
        1 atctatcata cccctgcacc ccggcaggga caacgctatc cgcctgcgcc atgcgtccta
       61 acgcagggtc ataccagcga gcgttgtaga acatcaaccc aaacccttcc gttgcggacg
      121 tggacggatc gtccatgtag gaatattgcg aggtgtaagt gtacctcgtc aacgcataag
      181 cggacggtat gaccgcaggg tcggaattct tccacgtata gcgcacttcg ccccagggtt
      241 tgtatctcat ctccgagacc tttacgcctg ctgtatcggt ggtgatggag gtgaaaccca
      301 aatggtctcc caggaagtat tcctcgctca gttatgtcaa ggcgcatcat tacgggacag
      361 agtagaagac ggactaaact tacggtttgg ttcgtctttt gtaaagtagt gcatctgtct
      421 ttaaaggtta atcctttctc aaaaatactg aaatcttctt tactaaccgt ccaaaaaagg
      481 ttttattttc accatattct tttatcaaag ttttagtatc tctttcgtac tcccttttat
      541 ggcgacgttc attcatggcg ttgaataata taaaaaaaac aaacccaatt cctactatag
      601 ctgaatcaat cacctttcca tttaatccgt atacaagtgc gaagccacaa aggaaactaa
      661 ccatcccata cagccacccg gatttttttc ttaagtcaag agaattagag gaaacaaaaa
      721 agataatcag cacatacaat acactactaa ctatagctat tgctttcata tgagaaatat
      781 cctttagggg caagacttta ttatcggctt atcattaaga aatgatgtaa tatcattaaa
      841 ataatcatga ttgtatcccg ccccgtatcc atcaataatg aattcaacaa caggatcttt
      901 tattaggagc ccggcgaggt gatcgtgtcc gcctgcgcaa accgtcccaa cacagggtca
      961 taccagcggg cattgtccct acgggagact tcgcactaga acatcaaccc aaatccctcg
     1021 gtgactccgc ttgtgctggg atcatccata ccccctacgg ggatgatatg ggaatattgt
     1081 cccgtaaacg tgaagcgggt cagggcatac gccggtgtgg tggtctgact cgccttccac
     1141 gtatagcgca cttcgcccca gggtttgtat ctcatctccg agatcttcgc gccgcttgta
     1201 tcggtggtga tggaagtgga acccaaatgg tctcccagga agtattccac gctcatttat
     1261 gccaaggcac acgaccagac ggtggcggag gattatttcc aagccatgca gagggtggag
     1321 cagagattaa acgcttcgac ggtgctcagc gcaaggtttg tgccagtaga aaagaatatc
     1381 aaggatgtta aggttcaaaa tgtttttcaa ctcattgaaa gtctggcatt gccagatctt
     1441 tgccaagaag agcgcttgga gattgtggga gcgctcaaga tggctttaga ttagttgttg
     1501 cactttaata attgttttct aaaatacgca actgattact tagcagagag acaaatacca
     1561 ttacaaaaag ttacgttgcc ctttatttaa tacacaccgt aattaaatta gagcttacaa
     1621 caccttccca gttattattc tctctctggt aatctgaata aacaaatcta atcaagtatt
     1681 gtcctgacgt tggagttgaa cgaatttcat cagtcttgtt tctatatatt tttttaggaa
     1741 aatagtaatc tgtagcgaat tttttagatg cattttggaa aattgttgag tattcattta
     1801 gaggaacgta aaatgtgtcg ctacttaggt ttctatcggc aagagtatag attcggtttt
     1861 tatggctgtc gaaaataatt gctgttaggt ttcctcctgc gcccaaaccg ccatttatta
     1921 tattgaaccc tgaagttatt aaaataggcc ctgttgttaa gttttgaaaa aacatattta
     1981 tttttatagg ctcaccggca ttattgcact cagatgaaat cacctcaaca gaaaacataa
     2041 gcctaccatc cgttacctgc ccatcaaagc tatacacgga c
//
LOCUS       DAOQMC010000336         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_36178_length_2074_cov_4.123328, whole genome shotgun sequence.
ACCESSION   DAOQMC010000336 DAOQMC010000000
VERSION     DAOQMC010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36178_length_2074_cov_4.123328"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PKV31_10035"
     CDS             complement(<1..787)
                     /locus_tag="PKV31_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93241.1"
                     /translation="MSSNKKNSKRGLPFRLGAQFISRGTSDLKAPEQFGHVLNKDQKK
                     KIRKLQWITYTISALIGATAVLIVILPYHLTAFFKPTHIEFWGISIDYDITYFVYAIA
                     IIFPEIWLLNVVNIRAVRTLCRIYRFPSVHSRDYNEQVDLLTEAGLEIPSSHMMSLGI
                     DPYVGMTRLSYYALFLFNKIKASLTNVAAKFIVRRLLGRYALRIVTDLVGVPIYAFWD
                     AWSSHLVIKETKLRISSYSAINDFMKDIRDEQLYAIRQKLPDLI"
     gene            1005..1349
                     /gene="arsC"
                     /locus_tag="PKV31_10040"
     CDS             1005..1349
                     /gene="arsC"
                     /locus_tag="PKV31_10040"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537942.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNB93242.1"
                     /translation="MKTIIYHNSRCSKSREGLCLLEELGEDIEIRNYLKEPPTYEELE
                     NLLTMLNAVPLDIIRQKEALFKELYEGKHLTHQEWIKAMIEHPVLIERPIVVKGNRAV
                     VGRPPARIRELL"
     gene            1449..1841
                     /locus_tag="PKV31_10045"
     CDS             1449..1841
                     /locus_tag="PKV31_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93243.1"
                     /translation="MENKSNDATPLRPEGERILNAPMVEMDLEKFIGQIRSESTWANS
                     DHNSITIYKSDIMRMVLIGLHKGSVIKPHQTSSAISVQVLEGLIRFHTEEKSIDLSKG
                     KMVALQPKIQHSVEALEESFFLLSFANG"
     gene            complement(1869..>2074)
                     /locus_tag="PKV31_10050"
     CDS             complement(1869..>2074)
                     /locus_tag="PKV31_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNB93244.1"
                     /translation="AVGMSTVPEVIVAVHAGMKVFAASIITDLGTPGHAGDISHEDVL
                     KAANEAAPKLAAIVREVVARMED"
BASE COUNT          594 a          398 c          466 g          616 t
ORIGIN      
        1 taatgaggtc gggaagtttt tgcctgatgg cataaagttg ctcatccctt atgtctttca
       61 tgaaatcgtt gattgccgaa tatgatgata ttcgcagttt cgtctccttg atgacaaggt
      121 ggctggacca tgcatcccag aaagcatata tcggcacccc aacaaggtct gtaacaatcc
      181 tgagggcata acgtcccaat aaccttctga ctatgaattt ggcagctaca ttggtcaggc
      241 ttgctttgat cttgttgaat agaaaaaggg cgtaatagga taatctcgtc ataccgacat
      301 aagggtcaat ccccagtgac atcatgtggc tggacggaat ttcaaggcct gcttccgtga
      361 gaaggtcaac ttgttcgttg taatctctgc tgtggactga cgggaatcga tagattctgc
      421 acagtgtcct taccgcacgg atattcacca catttaataa ccagatctcc ggaaaaataa
      481 ttgcaatagc atacacgaag tatgtgatgt cataatcaat ggatattccc caaaattcta
      541 tgtgtgttgg tttgaaaaag gcggtcaggt gatagggcag tatgacaatc aaaaccgctg
      601 tggcaccaat tagtgctgat atagtgtaag taatccactg aagtttcctt attttctttt
      661 tttgatcttt gttgagcaca tgcccgaatt gttcaggcgc tttcaaatcc gatgtgcccc
      721 ggctgatgaa ttgtgcgcca agtctgaatg gcagtcctcg tttgctgttt tttttattac
      781 ttgacatgct aaatgaagtc aaaaataaaa attgtcccga attgcacggt tcaaattatt
      841 tgcgatttta tggttagggc gcacctgatg aaaataagat aaattggtgt tttatatttg
      901 taaaaaattt attttgtgct aaaattcttt atctttataa ttcaatgggt tgtcgacact
      961 caggactgga atcatttttt tgaaatctga atttgtgtat ttgtatgaaa acaatcattt
     1021 atcataattc cagatgcagt aagagcaggg aaggtctttg tctattggaa gaattgggtg
     1081 aggatattga gatcaggaat tatctcaaag aacctccaac ttatgaggag ttggagaatc
     1141 ttctcacaat gctcaatgcc gtccctttgg atatcatacg gcaaaaagag gcactcttta
     1201 aggaattgta tgaaggtaaa cacctgacac atcaggaatg gatcaaggca atgatcgagc
     1261 atcctgtcct gatagaacgt ccaattgtgg tgaaaggcaa tagagctgtt gtaggcagac
     1321 cacccgcccg gatacgtgaa ctattgtgaa atgtccgggt gaaagagagt agaaaagtct
     1381 ttacggaatt gcttgattga aaacttagtt acctttaaat ttacctgata attcaaacat
     1441 aatgtcctat ggagaataaa tcgaatgatg caacacctct gagacctgaa ggagaacgta
     1501 tattgaatgc tccaatggtt gagatggatt tggagaagtt tatcgggcag attcgcagtg
     1561 aatcaacatg ggcaaacagt gaccacaatt ctattactat ctataaaagt gatataatga
     1621 ggatggtcct cattggattg cataagggct ctgtgatcaa accgcatcaa acatcttcag
     1681 ctatcagcgt tcaggtcctg gaaggattga tccggttcca tacggaggag aagtccattg
     1741 atttgtccaa agggaagatg gtggcgttgc agcctaagat tcaacatagt gtcgaggctt
     1801 tggaagaaag ttttttcctg ctttcgtttg ctaatggtta aatagccaat aaacatgagt
     1861 ctgcccggtt aatcttccat ccttgcaact acttctctga cgatagctgc cagttttggc
     1921 gctgcttcat tagctgcttt caatacatct tcgtgggaaa tatcaccggc atgtcccggt
     1981 gtgcctaagt ccgtgatgat gctggcagca aaaaccttca tgccggcatg tactgctacg
     2041 atgacttccg ggacagtgct catacccacc gcat
//
LOCUS       DAOQMC010000337         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_38869_length_1997_cov_4.737899, whole genome shotgun sequence.
ACCESSION   DAOQMC010000337 DAOQMC010000000
VERSION     DAOQMC010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38869_length_1997_cov_4.737899"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..1141)
                     /locus_tag="PKV31_10055"
     CDS             complement(323..1141)
                     /locus_tag="PKV31_10055"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNB93245.1"
                     /translation="MVKPGDRKNMAAYLMETFTVSTTRACKVIGMPKSMYYYQSVKSD
                     QPVIDKLNELVSMRVNHKAGQDKLYERIRTEGIKWNYKRVRRVYLAMGLKHRQRSRKR
                     VPKREKQPLVVPIARNMSWSMDFMHDTLMNKRRFRVLNIIDDYNRQALIVDGDYSFPS
                     EAVIRQLERAIHEYGKPQSIRVDNGPEFTCSTFLDWCNKMDIKIQFIQPGKPMQNGYI
                     ERFNRTFRENVLDAYMFKDLYEFNDIKDEWLEDYNHHRPHESLNNHSPIKYGTA"
     gene            complement(1135..1401)
                     /locus_tag="PKV31_10060"
     CDS             complement(1135..1401)
                     /locus_tag="PKV31_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB93246.1"
                     /translation="MKTTRFTESQIVQALKEYEGGKKAEALCRELGITKSTLYNWKKK
                     YAGLDGEQLRRLKELEEENRKLKSMYADLALDNKMLKDVLSKKW"
     gene            complement(1449..>1997)
                     /locus_tag="PKV31_10065"
     CDS             complement(1449..>1997)
                     /locus_tag="PKV31_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93247.1"
                     /translation="LTLGINAINVDPQKGVDKDSLDVKALLFPETGNFGILFNPSLLL
                     YSTTPIETDLDENSTNVVYHSWSFEGSFAYRNNLVNFKGNDTLPEEKITFENVNWTAG
                     FKYSYFKRIDKENNISFSLTAYYSAFNVPQEGVANFYKVLNDPIFKEKNTRGVINGFG
                     AKTEVQYNNFILFADVRYCNPP"
BASE COUNT          518 a          432 c          376 g          671 t
ORIGIN      
        1 ctaccacatt tttgtaaccg gtgagtaccc acccgcccca gcggtcgttc cagtaaaccg
       61 ggtcggcctg acgcagatgt cgataataac tatgaggatc cgcgatgaca tctttcgcca
      121 tcagatcatc gtgtacgagt gttgattcca tacaaggatt tcagataaat tttttaggtg
      181 gataaagaac ctgagaaaaa gggattttcc ctattgtaac cctccctaaa atcggactgc
      241 aatcaaaatt actaatttaa tttgatgatg tgctgttgat atgttggaaa cctttttcag
      301 gttttcaata tatcaatagc ttttaagctg tgccgtattt tattggagag tgattattta
      361 gtgattcatg tggtctgtga tggttatagt cttccagcca ctcgtcttta atatcattga
      421 attcgtataa gtccttgaac atatatgcgt ccagtacatt ctcacggaat gttcgattga
      481 atctttcaat gtatccgttc tgcatgggct ttccaggttg aatgaattgg atcttgatat
      541 ccattttgtt gcaccagtca aggaaggttg aacaggtaaa ttctggtcca ttatcaaccc
      601 tgatactttg cggtttgcca tattcatgaa tcgcacgttc cagctggcga atcaccgctt
      661 cactgggaaa gctatagtcc ccgtccacaa tcaaggcctg ccgattgtaa tcatcaataa
      721 tgttcaatac ccggaaacga cgtttgttca tcagtgtatc atgcataaaa tccattgacc
      781 agctcatgtt acgagctatg ggcactacca atggttgctt ctcgcgtttg ggcactcttt
      841 tacggcttct ctgacggtgt ttgagtccca tggcaaggta aactcttcgg acgcgtttat
      901 agttccattt gatcccctcg gtgcggatac gctcgtatag cttgtcctgg cccgctttat
      961 gattaacacg catagatacc agttcattga gtttatcgat gaccggttgg tcgcttttga
     1021 ccgattggta ataatacatg gacttgggca ttccgattac cttacaagct ctggtggtgc
     1081 ttaccgtaaa tgtctccatc aggtaggcag ccatgttttt gcgatcacca ggctttacca
     1141 cttttttgaa agaacatcct tgagcatctt attgtccaaa gccaaatcag catacataga
     1201 ttttagcttt cggttctctt cctccagttc ctttaagcgt cgtaactgct ctccgtcaag
     1261 gccagcatac ttcttcttcc agttgtacaa agtgcttttg gttatgccaa gctctcggca
     1321 caaggcttct gctttctttc cgccttcgta ctcttttaat gcctgtacga tctggctttc
     1381 tgtgaatcgg gtcgttttca tatcctgatt aaagttaagg ttttttcctt ttttaaccag
     1441 tccggatttt agggagggtt acagtatctc acatcagcaa ataaaatgaa attattatac
     1501 tgtacttccg tcttggcacc aaaaccattg attacacccc ttgtgttttt ctctttaaat
     1561 attggatcat ttagtacttt atagaaattc gcaacaccct cttgaggtac attaaatgct
     1621 gaataatatg ctgtaaggga aaaagaaata ttgttttcct tatctattct tttgaaataa
     1681 gaatacttaa acccagcagt ccaattcaca ttttcaaatg tgattttctc ctctggcagt
     1741 gtatcgttcc ccttaaaatt gaccaaatta tttctatacg caaatgatcc ctcaaaggac
     1801 caactatgat atactacatt tgtactgttt tcatctaagt cagtttcgat tggtgtagtt
     1861 gaataaagca ataaacttgg attaaataaa attccaaaat ttccggtctc tgggaatagc
     1921 agagctttaa catccaacga atctttatct acgccttttt ggggatcaac gttgattgca
     1981 tttatcccta aagtcaa
//
LOCUS       DAOQMC010000338         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_39050_length_1992_cov_5.475478, whole genome shotgun sequence.
ACCESSION   DAOQMC010000338 DAOQMC010000000
VERSION     DAOQMC010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39050_length_1992_cov_5.475478"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PKV31_10070"
     CDS             complement(<1..1320)
                     /locus_tag="PKV31_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNB93248.1"
                     /translation="MFRWLALVVFFTTALSVQALNVSILISQYYSIDKSLEPYLEVYL
                     HTIASTVDPLPSSSGDGLMEKKIKVLILFKQNNDIVNYDNFIIESGRKKDIEDFVTLK
                     RYGMKPGEYNIEFTLADLANTNNEYRDSVTVRINSAPEHPFLSNLQLVADTTSSADEH
                     IAKNGIFYELIPYGYMSPAINRFGLYFESYNTDQLATDIVNYHVKISKRINNTYKLVG
                     RTALKRRSARIDDILAWFDVTYYESGQYNIMVYLSDKDRNAIDSAEYYFTKSNPVRDE
                     AILSKKFMDEVETSFAGKLSRDQLNYTLRAIAMNVINSDVELVNRLIKDDNVIAKSNY
                     IYKYFKEKSALHPEEYYTQYMEVAKAVDKKYKTGFGYGFESDRGLIFMKYGKPSDMIS
                     ITDDPSCAPYEIWQYNEVPKLMQSNVKFLFFNPFLDGMDYRLLQSNAR"
     gene            complement(1337..1759)
                     /locus_tag="PKV31_10075"
     CDS             complement(1337..1759)
                     /locus_tag="PKV31_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93249.1"
                     /translation="MFRLFIAMFFFVAYSYGCVQPDSNVEVTPKMITGTWILDSALRD
                     GTPTESLDGLFYTFEQKKVVSNITGDTNETMFEIQGSKIVHLNDDSMVYQIETLNDSI
                     LKVSSNIHNIPFELKFRKFDSTTSTDEHGDLQESDGLK"
BASE COUNT          566 a          385 c          387 g          654 t
ORIGIN      
        1 tcttgcattg gactgtaaaa gcctgtaatc cattccgtcc aaaaaggggt tgaagaatag
       61 gaatttgaca ttggactgca tcagtttggg cacttcattg tattgccaga tttcatacgg
      121 ggcacacgaa ggatcgtccg tgatggatat catgtcgctg ggctttccgt atttcataaa
      181 tataagccct ctatcgcttt caaagccata gccaaatcct gttttatatt tcttatccac
      241 tgctttggcg acttccatat attgggtata atattcttca ggatgaagag ctgacttttc
      301 cttgaaatac ttatagatat agttgctttt ggcgattaca ttatcgtcct tgatcaatcg
      361 gttgaccagt tccacatctg aattgattac attcatcgca atggcgcgca aagtgtaatt
      421 caattgatcc ctggataatt ttcctgcgaa ggaagtttca acttcatcca tgaatttttt
      481 ggaaagaatt gcttcgtccc tcacaggatt actcttcgta aaataatatt cagcactgtc
      541 tattgcattt ctgtccttgt ccgacaaata taccatgata ttgtattggc cggattcgta
      601 ataggttacg tcgaaccagg ccaggatgtc gtcaatcctt gcagaacgtc ttttaagtgc
      661 agtacggccg acaagcttat aggtattgtt gattcttttg cttatcttga catggtagtt
      721 tacgatatca gtggcaagtt ggtctgtgtt gtaactttca aaatacaatc cgaatcgatt
      781 gattgcaggg ctcatatacc cataaggaat gagctcataa aagatgccgt tcttggcaat
      841 gtgttcatcg gcactggaag ttgtgtctgc cactaactgg agattagaca ggaaaggatg
      901 ttcaggggct gaatttatcc tgactgtgac actatccctg tattcattgt ttgtgtttgc
      961 caggtctgca agcgtaaatt caatattgta ttcccccggt ttcataccat accttttgag
     1021 ggttacgaaa tcttcaatat ccttttttct tccactttca atgatgaaat tatcgtaatt
     1081 tacgatgtcg ttattctgtt taaaaagtat cagtaccttg atcttttttt ccattaagcc
     1141 atcaccggag ctggaaggta aggggtccac ggtggaagca attgtgtgca aatagacttc
     1201 aagatatggc tctaaacttt tgtcaatgga ataatattgt gaaattagaa tgctgacatt
     1261 caatgcttga acgctcaacg cggtcgtgaa gaacaccact aaggctaacc aacggaacat
     1321 aattgattgt gtttatttat ttcagtccat cggactcttg aagatcacca tgctcatctg
     1381 ttgatgttgt actgtcaaat ttgcgaaatt tcaattcaaa aggtatgttg tggatgttgc
     1441 tgctcacctt aaggatactg tcattcaggg tttcaatttg atacaccatg ctgtcgtcat
     1501 ttaaatgtac tattttgctt ccctgaattt caaacattgt ttcattggtg tcgccggtaa
     1561 tattgctcac aacttttttt tgttcaaaag tataaaaaag accatccaat gactcggtag
     1621 gagtgccgtc tctgagtgct gagtcaagta tccatgttcc tgttatcatc tttggtgtaa
     1681 cctccacgtt agaatcgggc tgcacacagc cataagagta agctacaaag aagaacatgg
     1741 caataaatag cctaaacata aaattcaaat tggtttaaac tacaaaaata aatatttttg
     1801 gggacttgaa acatcgcttt gtgatttagt ctcaatcagt aatacataaa aaaaaccaaa
     1861 gtgttcaaaa tttgtcggtt aatcttgccc aatcaatgca ctttctttta tctttggcta
     1921 aataaattca attaaaaaac atagttacgg atgaaaagag aattgcacgc atggcatagc
     1981 ccggcgctca ac
//
LOCUS       DAOQMC010000339         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_39120_length_1990_cov_4.888889, whole genome shotgun sequence.
ACCESSION   DAOQMC010000339 DAOQMC010000000
VERSION     DAOQMC010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39120_length_1990_cov_4.888889"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..498
                     /locus_tag="PKV31_10080"
     CDS             58..498
                     /locus_tag="PKV31_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93250.1"
                     /translation="MKKGKNKTKELSTVKVLIGIEKKGKGASKTYAKVIGHPSKKKTQ
                     SFIEQKIDKQARICPDRNINTRLKNCKSSESEGRNKTLDQRIIRCMLNWVYDRHGYVT
                     HAQEYLNEYCYRYNRHQMKAAIIEDIIRRMVFHKPRPYKLMKYS"
     gene            complement(614..>1990)
                     /locus_tag="PKV31_10085"
     CDS             complement(614..>1990)
                     /locus_tag="PKV31_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNB93251.1"
                     /translation="EEFHNVTFSDEAIKACVRLSDRYISDRFLPDKAIDVMDEVGARV
                     HLKNIHVPKHIEEIEKQIEDIKEQKNLAVKNQQYEKAADLRDDESKLVRQLEEEKIKW
                     EDEAKTRRYPVDEDDIAEVVSMMTGIPLRRVAQNESNKLVNMNAEIRQMIIGQDAAIE
                     KVVKAIQRNRIGLKDAKKPIGSFIFLGPTGVGKTELAKALARHIFDSDDSLIRLDMSE
                     YMEKFSVTRLIGAPPGYVGYEEGGQLTEKVRRKPYSVILLDEIEKAHPDVYNILLQVL
                     DDGILTDGLGRKVDFKNTMIIMTSNIGVRQLKDFGTGVGFNTAARLESEEENTKNIIQ
                     SALKKTFSPEFLNRIDDVVIFNSLTKAEIFQIIDIALKSLYSRLSALGFSLELSEEAK
                     DFVAEKGYDPQFGARPLHRAIQKYIEDPLAEFILNENPQEGAKFSAVLNEDKTHIKIT
                     LSSEAKTA"
BASE COUNT          584 a          457 c          346 g          603 t
ORIGIN      
        1 ttctttgacc gttttttaac ataatttgtt gaataatgcg caacttgggt taatgtcatg
       61 aaaaaaggta aaaataaaac gaaagaacta agcactgtca aagttttaat tggaatagaa
      121 aaaaaaggca aaggtgcttc aaaaacctat gccaaagtaa taggccaccc atccaaaaag
      181 aaaacgcaat ccttcattga acaaaaaatt gataaacaag ccagaatttg cccggatagg
      241 aacatcaaca cacgattaaa aaattgtaag tcttcagaat cggaaggcag gaacaagacc
      301 ctagatcaaa gaatcatccg atgtatgctc aattgggtct atgatcggca tggttatgtg
      361 acacacgctc aggaatattt gaatgaatat tgctaccggt acaatcggca tcagatgaaa
      421 gcagcaatta ttgaagatat tattagacgt atggtttttc ataagccacg tccatataag
      481 ttgatgaaat actcttgaca aaatgaacga aatatcggct aattatttca gcttttatca
      541 aacatcattc aaaaacgatc aaaaaaagcc ctgaatctct tcaaggcttt ttcatgtcta
      601 ttctatacca atcttatgca gtcttggctt ctgatgataa tgtaatctta atatgggttt
      661 tgtcttcatt caagactgct gagaatttgg ccccttcctg cggattctca ttaagaataa
      721 actccgcaag aggatcctct atatatttct gaatggcacg atggagaggc ctggcaccaa
      781 actgaggatc atagcctttt tctgccacaa aatcctttgc ctcctcagat aattccaacg
      841 agaaaccaag agctgacaga cgactgtaaa gagacttcag agcaatatca ataatctgga
      901 aaatctcggc tttagtcaaa ctgttgaaga ttaccacatc atcaatccta ttgaggaatt
      961 ccggggaaaa agtcttcttc aatgcagact ggatgatatt ttttgtattc tcttcctctg
     1021 attccagtcg tgctgctgta ttgaatccga ctcccgtacc gaaatccttc aattgtcgca
     1081 ctcctatatt agaagtcatg ataatcatag tgttcttgaa atccaccttt cgacccaatc
     1141 catcagtaag gatgccatca tccagcacct ggaggagaat attatataca tccggatgcg
     1201 ctttttctat ctcatccagc aaaattactg agtatggctt ccttctgacc ttttctgtca
     1261 attgcccacc ctcttcatat ccgacgtaac ccggaggggc gccgatgagc ctggtcacgg
     1321 agaatttctc catatattca ctcatatcta atcggatcag actatcatcc gaatcaaaaa
     1381 tatgtcgtgc taatgctttc gctaattcag tctttccgac accggtaggt cccagaaaga
     1441 tgaatgatcc tattggtttt ttcgcgtcct tcaatcctat cctgtttcgc tggatagcct
     1501 ttacgacttt ctcaatggct gcatcctgac caatgatcat ttgcctgatc tcagcattca
     1561 tattgaccag tttgttactc tcattctgcg ctacccttct caatggaata ccggtcatca
     1621 tagacacgac ctccgcaata tcatcttcat cgacgggata ccgtcttgtt ttggcctcgt
     1681 cctcccattt gattttttcc tcttccaact ggcgcaccaa tttggactca tcatcccgca
     1741 aatcagctgc tttttcatat tgctgattct tgacagcaag atttttctgc tccttgatat
     1801 cctcgatctg tttttcgatt tcttcaatat gcttaggcac atgaatattc ttcaagtgaa
     1861 ctcttgctcc gacttcgtcc atcacatcaa tcgctttatc cgggaggaat cggtcgctga
     1921 tataacggtc acttaaccgt acacatgcct tgatggcttc atccgaaaaa gtaacattat
     1981 ggaattcttc
//
LOCUS       DAOQMC010000340         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_39444_length_1981_cov_4.855659, whole genome shotgun sequence.
ACCESSION   DAOQMC010000340 DAOQMC010000000
VERSION     DAOQMC010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39444_length_1981_cov_4.855659"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..594
                     /locus_tag="PKV31_10090"
     CDS             283..594
                     /locus_tag="PKV31_10090"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HNB93252.1"
                     /translation="METIKKWINIKGRASRTEFWGAMLFFITFMLVFGYIGELIEFRF
                     LPLYISPIGLIPFLATGMRRMQDVGKKGIFFFIPFYNFYIALTGGEKGPNQYGPNPRN
                     S"
     gene            620..1393
                     /locus_tag="PKV31_10095"
     CDS             620..1393
                     /locus_tag="PKV31_10095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB93253.1"
                     /translation="MNRPLSVVIIANNASRHIGEVIGAARRVSDDIIVLDSGSEDNTP
                     EIARDMGARVVFQDWLGYSATKNVGNSMAQHDMILSLDADEVLSEELIDHILIEDFNE
                     ETIYILDRANYYCGRRIRFCHWNPDRIPRIFDRRKARWQGEFVHEKLKFHTSYSQKLL
                     KGKLLHYSYDSYAQRLRKVKDYAILSARDRFRKGKRTTLPAMIVLPFFKFWITYIIHL
                     GILDGRAGLEIAWTDALGSWKRNRVLYDLQRNLTPECCK"
     gene            1753..>1981
                     /locus_tag="PKV31_10100"
     CDS             1753..>1981
                     /locus_tag="PKV31_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93254.1"
                     /translation="MDSAISAAHILASKHIPFCLYRLPGNEDKRIAIDSEVAGPHLIR
                     RFVVRPFSEESAAEPLIYTAMSRQNLNSDFIE"
BASE COUNT          579 a          402 c          401 g          599 t
ORIGIN      
        1 tggaatcaat cccaaaactt tgacttccct ttttctaatg cgtaatctgg gttaatcact
       61 agttatccgg tagtaagcat agttttttct ataagatata taatattgag gctgtctttt
      121 aaagctaaaa atccggttta attgttcctt aattcacttt gcattggagc ctgaaagaaa
      181 acgagcatct ctccatgtat ccaatcggct caaaatctcc tcattttatc tctttaattt
      241 gtacttttgc aatccgttat tatcaattaa aatgaatgcc ggatggagac aattaaaaaa
      301 tggatcaata taaagggcag ggccagtcga accgaatttt ggggagctat gctttttttt
      361 atcacattta tgttggtatt tggctatata ggtgagctca tcgaattccg gtttttacct
      421 ctatatatca gtcccattgg cctgatcccc tttttggcta caggaatgcg acgtatgcag
      481 gatgtcggca aaaaaggcat ttttttcttt attcctttct ataacttcta cattgcccta
      541 acaggtggag agaagggccc caatcaatat ggtcctaatc ccagaaatag ttgagtaatc
      601 tgaaatcaac attagagttt tgaaccgtcc cttgtctgta gtcatcatag ccaataatgc
      661 ttcacggcac attggcgaag tgattggagc tgcccgcagg gtgagtgacg acatcatcgt
      721 cttggattca ggcagtgagg acaatacacc cgaaatagcc cgggatatgg gtgccagagt
      781 cgtgtttcag gattggctgg gctatagcgc aacaaaaaat gtgggaaaca gtatggcgca
      841 acacgatatg atactttccc tcgatgcaga tgaagtatta tcggaagaat tgatcgacca
      901 catccttatc gaagatttca atgaagaaac catctatatt ctggacagag ccaattacta
      961 ctgtggtcgc aggattcgtt tctgccattg gaaccccgat cgtatccccc gaatatttga
     1021 ccgtcgaaaa gcaagatggc agggcgaatt tgtccatgag aaactgaaat ttcatacttc
     1081 ctattcccaa aagttactga aaggcaaatt gctccattat tcgtatgatt catatgcaca
     1141 acggcttaga aaggtgaagg attatgcaat actttcagca agggaccgct ttcgtaaggg
     1201 taaaagaact actcttcccg caatgattgt ccttccgttt ttcaaattct ggattacata
     1261 catcattcat ttaggtattc tggatggtcg ggccggcctt gagattgcat ggaccgatgc
     1321 cctcggatcc tggaaaagaa atagagtgct gtatgacttg caaagaaatc ttacgcctga
     1381 atgttgtaaa taacagtcgg atttatttaa aatcttaatc taaacagtag ctctgctgcc
     1441 gtatttaaga cggataacgg atctcaagac cgtacgctga accggattgt ttaaaggata
     1501 tttttataaa ccacaaaagg gacaactctt ttcctgactt ccgcactttt tacaactaaa
     1561 acactgatgt acaagtagtt gcattttcaa ttaggcacta caataaattt ataatcaatt
     1621 gacattcaca tagttggtgt gtttattgca ggaaagcact tgtatgctag agtctgcatg
     1681 tcattttgaa gaagtgaata gttacaaatc gcatattctt gctacttttg ccaatcaaat
     1741 ctttgagaat aggtggactc agccatttct gcagcgcaca tcctggcgtc aaaacatatt
     1801 cctttttgtc tttaccgttt acccggcaat gaagacaaac gcatcgccat tgattcggag
     1861 gtggcaggcc ctcaccttat cagaagattt gtcgtacggc cattcagtga agaatcagcg
     1921 gctgaaccat tgatatatac agcaatgagc cggcagaatc tcaattcaga ctttattgaa
     1981 t
//
LOCUS       DAOQMC010000341         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_40533_length_1954_cov_4.328067, whole genome shotgun sequence.
ACCESSION   DAOQMC010000341 DAOQMC010000000
VERSION     DAOQMC010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40533_length_1954_cov_4.328067"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKV31_10105"
     CDS             complement(<1..729)
                     /locus_tag="PKV31_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNB93255.1"
                     /translation="MAELNVRSISKELKAGIGKEVADIVASGARPPHLAAIMVGDNPA
                     SKAYVGNKVKTCEELGIKSTLLHFETGITQEELVEVIRRLNADDEVDGFILQLPLPDH
                     LDPQALLLEIDPNKDVDGFHPMNFGKMALSLPAFLPATPYGIVMLIEYLGLDLSGKKV
                     AVLGRSDIVGTPISLLLSRKAPYGNATVTLLHSRSKNIKEELLAADVIVAAIGIPHYV
                     KEDMVKEGSTIFDVGINRIPDSSKK"
     gene            1198..1680
                     /locus_tag="PKV31_10110"
     CDS             1198..1680
                     /locus_tag="PKV31_10110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB93256.1"
                     /translation="MDKILTSEALIRFSDCDPMGHLNNMRYLDYFLNAREDQLKQYAG
                     FDIYQHIKKTGQIWVMFQNKITYLKPVFYNHTVLISSRIIGMSKKSIKVEMQMKDAGT
                     GDLKCVLWMIAVYFDTLRNQSAEHQPEIEAMLQQYLSPVVQQTFDERVKYLQEKYRTN
                     "
     gene            1702..>1954
                     /locus_tag="PKV31_10115"
     CDS             1702..>1954
                     /locus_tag="PKV31_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93257.1"
                     /translation="MRLSRIFFLGTFMVLGMVGGAQDKIDYSRQENWASLPEKDDNAD
                     QLPGKNGVDRQEDYPVDVFFLYPTSFTGQVKNNQWNAPID"
BASE COUNT          612 a          429 c          379 g          534 t
ORIGIN      
        1 ttttttgctt gaatccggta tccggttgat accaacatca aagatggttg atccttcctt
       61 taccatgtct tctttgacat aatgcggtat gccaatagcc gcaacaataa catctgccgc
      121 aagcagttct tccttgatat ttttacttct tgaatgcagc aacgtcacgg ttgcatttcc
      181 ataaggagcc ttgcgggaca gtaacaggga aatcggcgtc cccacgatat cgctcctacc
      241 gagaactgcc accttttttc cggaaagatc cagtccgaga tactcgataa gcatgactat
      301 gccataaggc gttgccggaa ggaaagcagg cagacttaaa gccatcttgc cgaaattcat
      361 cggatggaag ccatccacat ccttattggg atctatttcc agtaaaagcg cctgaggatc
      421 aaggtggtca ggcaatggca actggagtat aaatccatcc acctcatcgt cagcattaag
      481 tcggcggatg acctcaacca actcttcctg cgtaatcccg gtttcgaaat gcaacagggt
      541 gctcttgata cccaattctt cacaagtttt caccttatta cccacatatg ctttactggc
      601 gggattgtcc cccaccataa tcgctgccag atggggtggc ctggccccgg atgccactat
      661 gtcagccact tccttaccta tgccggcctt cagttcctta ctgatcgatc taacattcaa
      721 ctcagccatt ttcaattcaa tttattccaa tcacaatgat aataatattt tatgcgaaaa
      781 cagaaataaa tgcacctgaa atagtaccaa tactttgttt taaaacaata ttaaaaaaca
      841 agcttataaa gtacctcata taattaccga tacgttgcat tcaaaatcaa tgcccttcgc
      901 aatgatttcc tttgaatcag acgggacagc cggaatcaac cgtcaaaatc tatgcaaaaa
      961 caggcaattt cctgttgatt ggagaatatc gaagcagtga aatcctttgg agaaagatga
     1021 caaacaggct ttgatctaaa aatctgtaga aatttttacc acaaaaacca accgaaacca
     1081 agcgattatc ctaaaataaa ggcatcgctc ttgctcatcc tgaagaaaat ctcattttca
     1141 catttgaagc ctaaacaatg gtactattta cttgtttgta tatccaaaat tgataatatg
     1201 gataaaattc tgacttcaga ggctttgatc cgtttttcag actgtgaccc aatggggcat
     1261 ctcaacaata tgagatatct cgattacttc ctcaatgcgc gggaagatca attaaagcaa
     1321 tatgccggtt tcgacattta tcaacatatc aaaaaaaccg gccaaatctg ggtcatgttc
     1381 cagaataaga tcacttatct gaaacctgta ttctacaatc atacagtcct tatcagtagc
     1441 aggataatcg gcatgagtaa aaaatcaatc aaggtggaaa tgcagatgaa ggatgcaggt
     1501 accggcgatc ttaaatgcgt gttgtggatg attgctgtct atttcgatac cttgcgtaat
     1561 caaagtgccg aacatcagcc tgaaattgag gccatgctac aacagtattt gtcacctgtg
     1621 gtgcaacaga cttttgacga aagagtaaag tatcttcagg aaaaatatcg gacaaattaa
     1681 attctataaa aggctaattt tatgagattg agcagaatat tctttttggg cactttcatg
     1741 gtgttgggga tggttggcgg agcacaagac aaaatcgact attccagaca ggaaaactgg
     1801 gcatcacttc ctgaaaaaga cgataatgct gatcagttac ccggcaaaaa cggtgtggat
     1861 agacaggagg attatccggt agatgtgttt ttcctatatc caacatcctt tacaggccag
     1921 gtaaagaaca atcaatggaa tgcaccgata gatg
//
LOCUS       DAOQMC010000342         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_41371_length_1932_cov_5.779968, whole genome shotgun sequence.
ACCESSION   DAOQMC010000342 DAOQMC010000000
VERSION     DAOQMC010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41371_length_1932_cov_5.779968"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKV31_10120"
     CDS             <1..572
                     /locus_tag="PKV31_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB93258.1"
                     /translation="IQTNSDTIASRLSTIDDGMVNGNVDDMGNWVAELGGEYTLQLPN
                     GKTLKVYENSIERQLVDFILTGQKDESVLKQKWFTFDRLYFRQGKSELTEDSKLQIDN
                     IAEILRAFTAVKIKMGGYTDSDGNEALNLKLSEERSNIARKELLKRGIASARVEAEGY
                     GESHPLCPANDTPACKARNRRIDIRITGM"
     gene            complement(718..1839)
                     /locus_tag="PKV31_10125"
     CDS             complement(718..1839)
                     /locus_tag="PKV31_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93259.1"
                     /translation="MSDKLNLANSSKILSSSRFEKQYLMSALSTRIFIVGLGETKSLN
                     AAIRYFKGLDKKYNGFTSPFYFDKVLKHNGKFFWNPFLPTFFSEAFERYIINEFENLK
                     EDQGLLLPGVSYIDFTSCGSQSRLNTTIDLFVTKKFKLYDLTHKLYIKWNQPRSMNYM
                     LARFLNVLDLQTEPWLVIDDNAYEYDYESLSAKGIKGVIINLGNEYSEQFATKNIGYN
                     NNPIATLIRNIRNAGLIAGINLGIRYAMEDNLQLVLKNSKDLGTHFMIWQYDCDDLDA
                     EQDQRKINQAAIERAFLKAGSSKEYEQFPLIFYPEFFEKINKNKSSIRKYAAYSFENG
                     NMIQHSGPYTSLFREILTIRKAKPYNTQYSGIYAPATKR"
BASE COUNT          577 a          381 c          380 g          594 t
ORIGIN      
        1 cgattcaaac caactccgac actattgcat cccgactctc cactattgat gacggtatgg
       61 tcaatggcaa tgtggatgat atgggcaact gggtggctga gttgggtgga gaatataccc
      121 ttcaactccc taatgggaag acactgaaag tgtatgaaaa ttccatagag cgccagttag
      181 ttgatttcat cctgacggga caaaaggacg aatccgtcct gaagcaaaaa tggtttacct
      241 ttgatcgttt gtatttccgg caagggaaat ctgaactaac agaggactcc aaacttcaaa
      301 tagacaatat cgcagaaata ctacgagcat tcactgcagt gaaaataaaa atggggggat
      361 ataccgactc cgatggcaat gaggcattga atcttaagct ttctgaagaa aggtccaaca
      421 ttgcccgaaa ggaattgtta aagcgcggca tagcatctgc cagggtcgaa gcggaaggtt
      481 atggagagtc acatccacta tgtccggcca atgatacgcc ggcatgcaag gcaaggaaca
      541 gacggataga tattagaatc accggaatgt aacagaagta tattcctgtc ttcaatgata
      601 taaatgaggc aaaatgtgat tgctcatcgc ctggcgatat acattctttt gtcattcctt
      661 tttttaagct cgcacttccg acaccaagct aggagtctgc caatctcgat ctgtccatca
      721 tctttttgtg gcaggtgcat atatcccgga atattgggta ttgtatggct tggcttttct
      781 gatggtcaaa atttcacgga aaagagaggt atatggaccg gaatgttgaa tcatattgcc
      841 attttcaaat gagtatgcag catacttccg gattgatgat ttattcttat ttattttttc
      901 aaaaaattca gggtaaaata tcaaaggaaa ctgttcatac tccttactac ttcctgcctt
      961 caaaaaggct ctctctatag cggcctggtt tattttcctc tgatcctgct ctgcatccaa
     1021 atcatcacag tcatactgcc aaatcataaa atgagtgccc aggtccttgg aatttttaag
     1081 caccaattga aggttatctt ccatggcata acggatacct aagttgatcc ctgcaatcaa
     1141 tccggcattt ctgatgtttc tgatcagggt agctatggga ttattattat acccaatatt
     1201 ttttgttgca aattgttctg aatattcatt tccaaggttg atgatcacac cttttattcc
     1261 tttggccgat aatgattcat aatcgtattc ataggcatta tcatcaatga caagccaagg
     1321 ctccgtctga agatccaata cattcaggaa tcgggcaagc atatagttca tggatcgggg
     1381 ttgattccac ttgatataca atttatgggt caggtcatac aatttgaatt tttttgttac
     1441 aaaaagatct atcgtagtat taagtcgact ctgactgcca caacttgtaa aatcgatata
     1501 agaaaccccg ggtaacaaca gtccctggtc ttccttcagg ttttcaaatt cattgatgat
     1561 gtatcgttcg aatgcttcac tgaaaaaagt cggcaaaaat ggattccaaa aaaatttacc
     1621 attatgtttc agcactttgt caaagtaaaa tgggctggta aatccattgt atttcttatc
     1681 aagtcctttg aaatacctga tggccgcatt gagacttttt gtttccccaa gaccaacaat
     1741 gaatattctg gtagaaagcg ccgacattag atattgcttt tcaaaccggc tcgaactgag
     1801 aatctttgaa ctgttcgcca ggtttaattt atcggacata gttttctttt ccatatttaa
     1861 aaatcaattc tattatttca agcaatttga ttaatttgcg attaagtgag aaaatattga
     1921 ctgattttct at
//
LOCUS       DAOQMC010000343         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_41660_length_1924_cov_5.202247, whole genome shotgun sequence.
ACCESSION   DAOQMC010000343 DAOQMC010000000
VERSION     DAOQMC010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41660_length_1924_cov_5.202247"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PKV31_10130"
     CDS             <1..413
                     /locus_tag="PKV31_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNB93260.1"
                     /translation="SRGVCQVDEQLQLVTINERTKIGPENGRILYVDGNEKVEVPADS
                     FVSMNFWGFHPDVFPLFRKGFVHFLKYSNPTEKSEYFIPLVVDMMIKMKLSRFEVIPS
                     NSEWIGVTYQEDREPTVRKFAEFGKQGIYPEELF"
     gene            766..957
                     /locus_tag="PKV31_10135"
     CDS             766..957
                     /locus_tag="PKV31_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNB93261.1"
                     /translation="MQEGKVKFYNETKGFGFIKPNDGSADIFFHVSGVKEDVKDNDPV
                     VYDVEQGKKGLIAVNIRLA"
     gene            1464..>1924
                     /gene="ribB"
                     /locus_tag="PKV31_10140"
     CDS             1464..>1924
                     /gene="ribB"
                     /locus_tag="PKV31_10140"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNB93262.1"
                     /translation="MLNSIPEAIEAIRNGELIIVVDDEDRENEGDFIGAAELITPEKV
                     NFMATVGRGLICTPLEADRVEALGLDMMVNQNTAMHETAFTVSIDLIGHGCTTGISAY
                     DRATGIKALTDPKITGNDYAKPGHIFPLRAKAGGVLRRTGHTEAAVDLARMA"
BASE COUNT          579 a          331 c          438 g          576 t
ORIGIN      
        1 tgtcgcgcgg tgtatgccag gtggatgaac aattacaact tgtgaccatc aacgaaagga
       61 cgaagattgg cccggagaat ggccgtatcc tttatgtcga tggcaatgaa aaggttgaag
      121 taccggcaga ctcctttgtt tccatgaatt tctggggctt ccatcccgat gtattccctc
      181 tttttagaaa ggggtttgtc cacttcctga aatattccaa tcctacagaa aagtctgaat
      241 atttcatacc tttggtggtc gatatgatga tcaagatgaa gttgagtcgt tttgaagtca
      301 taccttccaa cagtgaatgg atcggtgtga cttaccaaga agaccgggag ccgactgtca
      361 gaaagtttgc agaatttgga aaacagggta tttatcctga agagttgttc tgaggaatcc
      421 cccgattata attcttaact catatagcct ggtattagag atctatatac ctgtaattgt
      481 attgtttcat tgaaattgaa tggaaacaat agcgcgtagt acgatttgta caaatgaaaa
      541 aacaggatta gaaaaattaa gttttgtgtg gtacctccct tgtataatta agcagttcag
      601 acgatgaatt aataaaaaac aggaaagtat taaaagttgg taagtttggt gtgcagttgc
      661 acttttaatg agccaaactc cgtttgagtg aagtaaaatt caacgacgat gctgataccc
      721 atgaaattgt aaattaataa aatattaaac attaaatgaa ttattatgca agaaggtaaa
      781 gtaaagtttt acaacgaaac aaaaggtttt ggttttatta aacccaacga tggttctgct
      841 gacattttct ttcacgtttc cggggttaag gaagatgtta aagataatga tccggttgta
      901 tatgatgtcg agcaaggtaa aaagggatta attgctgtca atataaggtt ggcgtaaggt
      961 agaactaaat acgtctttta gcaggttatt gggatgattg attctgtgtc cacgacacgg
     1021 atatcagacg ttccaattat gctgccgaaa tatccatttt agagggaaaa aagatagtgt
     1081 gtattgtgaa tgcaggtcag ccgatactca ctttactcta ttagctatta atttcaaaaa
     1141 atcagtacca caacattttt catggtcttt ctgataaatt tggccaattg aatggtgccg
     1201 gattcaggcc attgcttata ttgacattca ggaagcaatt gcatcaagca tgtatcttac
     1261 agctatgatt gtacaacggt tatgccaata agtcgcgttg tcaggtggtc ataatcgtcg
     1321 gatttgttct tctttaaggt ttatataccc tgatatgcac acgtggaaga tatcgtttta
     1381 ttattgcctg actctcaaaa ttattctttc atgccattat ttgccctaaa caatccgtat
     1441 ctttgcgcct caaaaatcag agcatgttga acagtattcc ggaagctatt gaagccatca
     1501 gaaatgggga actaattatt gttgtggacg atgaggatcg tgagaatgaa ggcgacttca
     1561 ttggggcagc tgaattaatt actcctgaga aggtaaattt tatggcgact gtgggccgtg
     1621 gcttaatatg cacccctctg gaagctgatc gggtagaagc attgggtctg gatatgatgg
     1681 tcaatcagaa taccgcaatg catgagacag ccttcactgt gtccattgac cttatagggc
     1741 atggttgtac cacagggatt tccgcttatg atcgggcaac aggtatcaag gcattgaccg
     1801 atcctaaaat taccggcaac gattatgcaa agccaggaca tatttttccc ttaagggcga
     1861 aggcaggtgg tgtattacgc aggaccgggc atacggaagc ggctgtggat cttgcaagaa
     1921 tggc
//
LOCUS       DAOQMC010000344         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_42042_length_1915_cov_2.972581, whole genome shotgun sequence.
ACCESSION   DAOQMC010000344 DAOQMC010000000
VERSION     DAOQMC010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42042_length_1915_cov_2.972581"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="PKV31_10145"
     CDS             <1..1392
                     /locus_tag="PKV31_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HNB93263.1"
                     /translation="HGDKIGRTLGFPTANLEITEQHKALPKDGIYLTKVIIEDKIHHG
                     LAYIGHRPSINSDLLRAVEVYIYDFDSDIYGSQVTLKLIDFIREDRKIDGLDALKAQM
                     EEDKEAGRRLITGITPSSKNLAVVVLNYNGAVHLRKYMPDLIKYSPQADIVLADNCST
                     DDSLDYLKTLEKDIKIIMLDQNYGFTGGYNRAIASISHPYVLLLNSDVRVTEDWLIPL
                     LSLIEEDPSIGAIQPKMLNDREPALFDYAGASGGWMDRLGYPFCRGRIFGDIESDGKQ
                     YDDRAEIFWATGAAMLTRTELYKKLGGLDEYFFAHMEEIDLCWRMKNAGYKIMVEPAS
                     KVYHYGGGTLDASSPRKTYLNFRNSLFVLLKNEQHKVWSKIFARLCLDGIAGIRFFLQ
                     GKFSFVSAILKAHFNFYANIGRYMKARKLITVQPLNSVRPLTGKYDGLIISQYFIKGK
                     KKFSDIIIENNES"
     gene            1382..>1915
                     /locus_tag="PKV31_10150"
     CDS             1382..>1915
                     /locus_tag="PKV31_10150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB93264.1"
                     /translation="MSPDIIFEDDHLIIVSKPSGILSIPDRHDPSKANMQDILSRKYG
                     KIFTVHRIDRETSGIMCFAKTAEVHAALSNMFQQREIEKTYLAFVSGNVQPEEGSIEA
                     ALMPDPLHSGRMKVSPTGKKSLTTYKVIERFKNCCLVEAGILTGRMHQIRVHFKYIGH
                     PCLVDKLYGTNEAIFIRD"
BASE COUNT          597 a          390 c          423 g          505 t
ORIGIN      
        1 catggcgaca aaattgggag aacactaggt tttcctacag ccaaccttga aatcactgag
       61 cagcataagg cacttcctaa agatggtatc tatctaacaa aggtcattat cgaagataag
      121 atacatcacg gactggcata tatcggccat cgaccatcca tcaacagcga tttgctccgt
      181 gccgtagagg tctatatcta tgatttcgat tctgatattt acggctcaca agtaacattg
      241 aagttgatcg attttatacg ggaagataga aaaattgatg gcctggatgc gctaaaagca
      301 cagatggaag aggataagga agccggaaga cggttgataa caggaatcac tccatccagt
      361 aagaatctgg ctgttgtagt attaaactac aatggtgcag tccacctcag aaaatacatg
      421 cctgatctaa tcaaatattc gccccaggca gatattgtct tggccgacaa ttgttccact
      481 gatgattctt tagattacct taagactttg gaaaaggaca taaagatcat aatgctggac
      541 caaaactatg gatttacagg tggatacaac cgggcaatag cttctatttc ccatccttat
      601 gtactcttat tgaattctga tgtacgggtc actgaagatt ggctcatccc tttactaagc
      661 ctgatagaag aggacccttc cataggcgcg atacaaccca agatgttaaa tgacagagag
      721 cctgcgttat ttgactatgc cggggcatca gggggttgga tggacagact gggatatccg
      781 ttttgcagag gtcggatctt cggagacatc gagtctgacg ggaagcaata tgatgacaga
      841 gcagaaatat tctgggccac cggtgcagct atgctaacac gtacggagtt atataagaaa
      901 ttaggcggac tggatgaata tttttttgct catatggaag agatcgactt gtgctggcgt
      961 atgaaaaacg caggttataa aatcatggtt gaaccggcct ccaaagtcta tcattatgga
     1021 ggtggcacat tggacgcatc ctcaccgaga aagacctatc ttaacttccg caacagtctg
     1081 tttgtattgt taaagaatga acagcacaaa gtctggagta agatattcgc ccgactttgt
     1141 ttggatggta tcgcaggtat tcgttttttc ctacagggca aattttcctt cgtaagtgcc
     1201 attcttaagg cacattttaa tttctatgca aatataggtc ggtatatgaa ggcaaggaag
     1261 ctcattaccg ttcagccatt gaattcagta agaccattga ccggcaagta tgatggcctg
     1321 atcatcagtc aatattttat caaaggaaaa aagaaatttt cagacatcat aatagaaaac
     1381 aatgagtcct gacatcattt tcgaagacga ccatttgata atagtctcca aaccatcggg
     1441 tatattgtct attccggaca ggcatgatcc ctcaaaagcc aacatgcagg acatactgtc
     1501 acgaaagtat ggaaaaatat tcaccgtgca ccgcattgac agggaaacat caggcatcat
     1561 gtgctttgct aaaaccgcag aagtacatgc tgcactcagt aatatgtttc agcaaagaga
     1621 aattgaaaaa acctacctgg cctttgtctc aggtaacgtt caacctgaag aagggagtat
     1681 tgaagccgcg ttgatgcctg acccgctcca ttccggtcgt atgaaagtca gccctaccgg
     1741 aaaaaaatca cttacaactt ataaggtcat agaaagattt aaaaactgtt gcttagtgga
     1801 ggcaggtatc ttaaccggac ggatgcatca gatccgggta catttcaagt acataggcca
     1861 tccgtgtctg gtcgacaaac tttatggtac taacgaagct atatttatcc gagat
//
LOCUS       DAOQMC010000345         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_42062_length_1914_cov_4.413663, whole genome shotgun sequence.
ACCESSION   DAOQMC010000345 DAOQMC010000000
VERSION     DAOQMC010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42062_length_1914_cov_4.413663"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PKV31_10155"
     CDS             complement(<1..1242)
                     /locus_tag="PKV31_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB93265.1"
                     /translation="MASLSTISVNRPVLAIVMNLIILLFGLIGFKTLGVREFPAVDPP
                     IINVRTNYPGANADIIESQITEPLEKAINGIQGIRTITSASNQGSSNITVEFDLSSDL
                     ESAANDVRDKVSQAIRQLPKDLDGVPIVSKADANSDAIISMTVQSNTRNHMELSDFAE
                     NVLGPRLQTIPGVSSLQIWGQKKYAMRLWLDPVKLSSFHLTAIDVTNALQKQNVELPS
                     GKISGDETELTIKTIGRLSTPEEFNNMIIKSFPDGRDIKLKEIGEAMLGVENEETILR
                     EKNIPMIALALVPQPGSNYVAIADEFYKRLDQIKKDVPKDISLNIAMDNTRFIKQSIV
                     EVEETFLIALSLVVLIIFLFFRSWIVAVRPLIDIPVSLIGSFFVMYLMGYSINVLTLL
                     AIVLATGLVVDDGIVVTENIYK"
     gene            complement(1245..>1914)
                     /locus_tag="PKV31_10160"
     CDS             complement(1245..>1914)
                     /locus_tag="PKV31_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB93266.1"
                     /translation="LAIQGTSKEDYNIAHNEVLSFKGDIAYYQTLIDKTELRAPFSGI
                     VGFRNIALGSYVSPSVVVTTLQQLDPIKIEFTAPEKYLANFKVGQKFNFYSEASKKTL
                     TGTVQTIDPQIDLNTRSVIIRGVAPNVANLLPGNFVKVDINISQESDVLRIPSSAVVP
                     ILKGQKVYLYKNGKVVEKKIEILGRDDKTVQVSNGLNAGDSLIITGIIMLKEDMDVKP
                     DKVK"
BASE COUNT          499 a          408 c          414 g          593 t
ORIGIN      
        1 tttatagata ttttctgtca ccacgattcc gtcatctacg acaagtccgg ttgcgagaac
       61 gattgccagt agtgtaagta cgttgataga gtatcccatt aagtacatga cgaagaaaga
      121 acctatcagc gaaaccggaa tatcgatcag tggtctgaca gcaacgatcc agctcctgaa
      181 aaacaggaaa atgataagga cgacgaggct taatgcaatc aggaaggttt cttccacttc
      241 cacaattgat tgtttgataa atcgggtgtt atccattgcg atattcaggc tgatatcttt
      301 tggaacatcc tttttaattt gatcaaggcg tttgtaaaac tcgtcagcta tggcgacata
      361 gttagatccc ggctgtggca ccaaagcaag agcgatcatc ggaatatttt tttcgcgcaa
      421 aattgtctct tcgttttcta cacccaacat tgcctcaccg atttccttca gcttgatatc
      481 tcttccatcg ggaaagcttt tgataatcat gttgttaaat tcttcgggtg tactcaacct
      541 gccaatggtc tttatagtca actccgtttc atctccggaa atcttaccgc tgggtagctc
      601 aacattttgt ttttgcagtg cattggttac atctatggcc gttagatgaa aggatgataa
      661 tttgacaggg tcgagccaaa gacgcatagc atactttttc tgtccccaaa tctgaagaga
      721 tgaaactccg gggatggttt gcagtctcgg acccaacaca ttctccgcaa aatcactcaa
      781 ttccatatga ttgcgtgtat tgctctgtac cgtcatggaa attatggcat ccgagttggc
      841 atctgctttg gatactatcg gtaccccatc cagatcttta ggcaactgcc ttatagcttg
      901 tgagactttg tcccggacat cattggcagc actttccaaa tcggaggaaa ggtcaaactc
      961 tacagtgatg ttggaacttc cttgattgga ggccgaagta atggtacgga ttccttgaat
     1021 accgttgatc gctttctcga gcggttcagt tatctgggat tcaatgatat cggcattcgc
     1081 cccgggatag ttggttctta cattgatgat gggagggtcg acagccggaa attctcttac
     1141 acccagtgtt ttgaagccaa tgagaccaaa taggaggatg atcaggttca tgacaatagc
     1201 cagaaccggt cgattgacgc tgatggtaga aagactggcc atggttattt tactttatcc
     1261 ggtttgacat ccatgtcttc cttaagcatg ataatgcccg taatgatcag gctgtcacct
     1321 gcattcagcc cattgcttac ctgaacggtc ttatcatccc tgcctaatat ttcgatcttt
     1381 ttttcgacta cttttccgtt tttgtagaga tagactttct gacctttaag tattggaaca
     1441 actgctgaag aagggattct gagaacatcg ctctcttggg aaatattgat atctaccttg
     1501 acaaaatttc cgggcaagag attggcgaca tttggtgcaa cacctctgat gataacggat
     1561 cgagtgttga gatctatttg tggatcaatg gtctgcacgg ttccggttag tgttttttta
     1621 cttgcctctg aataaaaatt aaatttctga ccgactttga agttggcaag gtatttttca
     1681 ggtgcagtga attcaatttt gattggatcc agttgttgaa gtgttgtaac tactactgaa
     1741 ggacttacat agctgcccaa tgcaatgttc ctgaatccga cgattccact gaatggtgca
     1801 cgtaactcag tcttgtcaat cagggtttga taataagcaa tatccccctt aaagctcaat
     1861 acttcattgt gggcgatatt ataatcctcc ttgctggttc cctgaatagc cagc
//
LOCUS       DAOQMC010000346         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_42554_length_1903_cov_3.614177, whole genome shotgun sequence.
ACCESSION   DAOQMC010000346 DAOQMC010000000
VERSION     DAOQMC010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42554_length_1903_cov_3.614177"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKV31_10165"
     CDS             <1..408
                     /locus_tag="PKV31_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93267.1"
                     /translation="GNIQENVVSFYEAVSRKNQGLPIIDRSYNQHLGWQFLFTMKQNE
                     LFVFPNAQTGFDPLNIDLFDAENKKIISPNIFRVQKIAQKDYFFRHHLETNVENNPAT
                     KNFTWKREGTLGLKGIIKVRVNHLGDIVSVGEN"
     gene            464..1360
                     /gene="cas1"
                     /locus_tag="PKV31_10170"
     CDS             464..1360
                     /gene="cas1"
                     /locus_tag="PKV31_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HNB93268.1"
                     /translation="MIKRTLYFGNPAYVSVRHKQLCVRKPGEDAEKTVPIEDIGMVIA
                     DHPRITITQTTVTSLQENNAVILWCGPDHLPVSMSLPFAGSDTYTERLRYQLAASEPL
                     KKQLWKQTIEAKLANQAKVLKRLGSPSPTIERMISKIGSGDPENYEGRAAAIYWQSIL
                     TIPYSTNRGRDEGGVNALFNYGYALLRAVIARNLVSSGCLPALGIHHRNKYNAFCLAD
                     DIMEPYRPVVDLYIVAFLEENPEFDGELTTDIKKYLLQIPVLDINIKGKNSPLMVGAS
                     RTTASLMDCFIGKARKILYPEL"
     gene            1357..1695
                     /gene="cas2"
                     /locus_tag="PKV31_10175"
     CDS             1357..1695
                     /gene="cas2"
                     /locus_tag="PKV31_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNB93269.1"
                     /translation="MMINRYSKYQIMWVFVHFDLPTETKRDRKNYAAFRKFLLEDGFK
                     MIQFSIYARHCSSRENAAVHKKRVKKALPPKGHVIVFDITDAQFGKMEIFYSQKSAPP
                     KDMPVQLELF"
BASE COUNT          586 a          362 c          437 g          518 t
ORIGIN      
        1 ggaaatattc aggagaatgt tgtttcattt tacgaagcag tctccagaaa aaatcaggga
       61 cttcccatta ttgacaggtc ttataatcag caccttggat ggcagttttt gtttacgatg
      121 aaacaaaatg aactatttgt ttttccaaat gcgcaaaccg gatttgatcc attgaatatt
      181 gatttatttg atgctgagaa taaaaaaatt attagcccaa atatttttag ggttcaaaaa
      241 attgctcaaa aagattattt ctttaggcat catttggaaa caaatgttga gaataatcca
      301 gcaacgaaga attttacttg gaaacgtgaa ggaacattgg gattaaaagg aataataaaa
      361 gtaagagtta atcatctagg tgatattgtt tcagtgggag aaaattaggt aattaatagt
      421 ttggcaaggt ttttgcatat aagtgaatgc agctaccttc ataatgatta agcgaaccct
      481 ttatttcggt aatccggcat atgtatccgt cagacacaaa caactttgtg tgagaaagcc
      541 gggtgaagat gctgaaaaga ctgttccgat cgaagatatc ggcatggtca tcgcagatca
      601 tccccggatt accatcacac agactacggt aacgtcattg caggaaaaca atgccgtgat
      661 actctggtgt ggcccggatc atctgccggt ttcgatgagt cttccctttg ccggcagcga
      721 cacctatacc gaaagactca gatatcagct ggcagcgtcc gaaccgttga aaaagcagct
      781 ttggaaacag accatagaag ccaagttggc caaccaggcc aaggtattga aacgacttgg
      841 atcgccatct ccaacaattg agcggatgat atcgaagatt ggcagtggag atcctgaaaa
      901 ctatgagggt agggcagcgg ccatctattg gcaatcaatc ttaaccatac cttacagcac
      961 caaccggggc cgggatgaag gtggcgtcaa tgctcttttc aactatggtt atgctttgct
     1021 gcgagctgta atagcccgca atttggtgtc cagtggatgc cttccggcct tgggcatcca
     1081 tcacaggaat aagtacaatg ctttttgtct ggctgatgat atcatggagc catatcgtcc
     1141 tgtcgtggac ctgtacatcg ttgcctttct tgaggagaat cctgagtttg acggcgagtt
     1201 gacgactgac atcaagaaat atcttcttca gatacccgta ctggatatca atatcaaggg
     1261 caagaacagc cccctgatgg tgggcgcttc ccgtaccact gccagtctga tggattgctt
     1321 catcggaaaa gcgcgaaaaa tattatatcc ggaattatga tgatcaacag gtacagtaaa
     1381 tatcagatta tgtgggtatt cgttcatttt gacctgccta cggagaccaa acgggacagg
     1441 aaaaactatg cggcttttcg taaattttta ttggaggatg gtttcaagat gattcaattc
     1501 agcatctatg ctcgccattg cagcagccgt gaaaatgctg cagtccataa gaaaagggta
     1561 aagaaggcat tgcctcccaa ggggcacgtg attgtattcg acattacgga tgcacaattt
     1621 ggcaagatgg aaatatttta cagccaaaaa tctgctccgc ctaaggacat gccggttcag
     1681 ctggaattgt tttgattctg gttgaaataa aaaaagccgg aaatttccgg cttttatatg
     1741 atttgctaca agaaatttgc tatccaaaga tgagttgtaa ataattgaaa gataggactt
     1801 tgcgacaggg tatgttgtag caaatatcaa tctttcaaag aacaaaagca attcacaact
     1861 ggcattagtg gagcgataga aaaggacggg ttgtagcaaa tat
//
LOCUS       DAOQMC010000347         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_43175_length_1888_cov_5.817785, whole genome shotgun sequence.
ACCESSION   DAOQMC010000347 DAOQMC010000000
VERSION     DAOQMC010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43175_length_1888_cov_5.817785"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1457
                     /locus_tag="PKV31_10180"
     CDS             69..1457
                     /locus_tag="PKV31_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB93270.1"
                     /translation="MAKKSKRFWWLIGLGGIVIILIIISIIQKRNRPKGVSVTTELVA
                     KHDIREIVTASGKIFPENEVKISSDVSGEIIALYVREGDTVKAGQLLAKINADTYLPS
                     VQRGEASVKSSKAQENISDTQIKAAKAQQEEVNARLANAQKVHERNKKLFEDGVISQA
                     DLEASQSSLDGLIASAKSAAASLRQAQENASASKYGTMSARANLDELKTSLSKTFIYA
                     PVDGVISSLNVEKGERVVGTIQMSGTEMMTIANLNSIEVQVEVSENDIVRVEKGNPAT
                     IEVDAFYGRKFNGVVTEIANSSTASSSTNTTASLNTDQVTNFIVKIRMDLNSYKDMII
                     KAKPFPFRPGMSASAEINTRTEHDVLSVPIQAVTTREDPSGKRNEKSGKKADLIEVVF
                     LQQGDTVNMVPVKTGIQDDEYIHILSGLKEKDRVVTGPYSTVSRTLKQGSVVHEDTKK
                     KGLDKEDKEEEQ"
     gene            1668..>1888
                     /locus_tag="PKV31_10185"
     CDS             1668..>1888
                     /locus_tag="PKV31_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNB93271.1"
                     /translation="MNSFNRALNIAGWLTFAITFMVFVFAVERTGSLWDCGEFILGAY
                     KLQVVHPPGAPLFLMIGRMFAYIGSMFTST"
BASE COUNT          594 a          370 c          472 g          452 t
ORIGIN      
        1 gaaggtattg gattattatg cagggcgaca aattatccta gaatagaatt tatcaatgaa
       61 aaaaagatat ggctaagaaa agcaagcgtt tttggtggtt gataggcctt ggaggcatcg
      121 tgataatatt gataattatc tccataatcc aaaaaagaaa taggccgaaa ggagtcagtg
      181 tgacgactga actcgtcgct aagcacgaca tccgggaaat tgtaacagct tccggcaaaa
      241 ttttcccgga gaatgaagtg aagataagtt cggatgtgtc cggagagatt attgccttgt
      301 atgtgcgtga aggagatacg gtaaaagcag gtcaattgct ggccaagatc aatgccgata
      361 cctaccttcc atccgtccag cgtggtgagg catctgttaa gagttcaaag gcacaggaaa
      421 acatttccga cactcagatt aaggctgcca aagcacaaca ggaagaggtc aatgcacgtc
      481 ttgccaatgc acaaaaagtg catgaaagga ataagaagtt gtttgaagac ggggtaatca
      541 gtcaggcaga tttggaggcg tcccagtcca gtttggatgg cctcatagca tctgccaagt
      601 cagctgctgc ttcccttaga caggcacagg aaaacgcctc ggcttcgaaa tacggaacca
      661 tgagtgccag ggctaacctg gatgagctaa aaaccagcct cagcaagact tttatctacg
      721 ctcctgtcga tggtgtcatc agttctctaa atgtcgaaaa aggcgagcgg gttgtgggaa
      781 caatccagat gtcaggcaca gagatgatga ccattgccaa tctgaactcc atagaagtgc
      841 aggtggaggt ttcagaaaat gatatcgtcc gggtagaaaa gggcaaccct gcaacaatcg
      901 aagtggatgc cttctatggc agaaaattta atggtgttgt aacagagatt gccaacagct
      961 ccaccgcttc cagcagtaca aacactactg cttccctgaa tacggaccag gtgaccaatt
     1021 ttattgtaaa gatcagaatg gacctcaact cctacaagga tatgattata aaggccaaac
     1081 ccttcccctt ccgaccaggc atgtcagcat cggcagagat aaatacaaga actgaacacg
     1141 atgttctgtc cgtgcccatt caggccgtga caacaaggga agacccttca ggcaaaagaa
     1201 atgaaaaaag cggaaagaag gccgacctta tagaggttgt cttccttcaa caaggggata
     1261 ctgtcaatat ggtaccggtg aagaccggaa tacaggacga tgagtatatc cacattctga
     1321 gtggattgaa ggaaaaagac agggtggtga ccggaccgta tagtaccgtg tcaaggacct
     1381 tgaaacaggg tagtgtggta catgaagata cgaaaaagaa agggttagac aaggaggata
     1441 aagaggagga acaataacgt gttaacttaa tattaatata taatcaccga tagctacgtt
     1501 ataaaaagag aggataattt gtccaataaa agaaagggct taactttgcc accaggtttt
     1561 tttgcccttt agtcgggtat accccggaag gatacagtgc tgttcgctaa aggtggaatc
     1621 ctgtggcatt gagtagttgc ctttttttat aaactgaaag ttaacggatg aatagtttca
     1681 acagagcctt aaatattgct ggctggctaa catttgcaat caccttcatg gttttcgttt
     1741 ttgccgtcga gaggaccgga agtctatggg attgcggtga atttattcta ggagcgtata
     1801 aacttcaggt ggtgcaccca ccgggagcgc ctttattcct gatgatagga agaatgtttg
     1861 cgtatatagg cagtatgttt acctccac
//
LOCUS       DAOQMC010000348         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_43397_length_1883_cov_4.187090, whole genome shotgun sequence.
ACCESSION   DAOQMC010000348 DAOQMC010000000
VERSION     DAOQMC010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43397_length_1883_cov_4.187090"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="PKV31_10190"
     CDS             <1..1809
                     /locus_tag="PKV31_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93272.1"
                     /translation="YIKLVYADSKFFPTACLSGKLTIMSLNNSGKKELEAPNIIFQNL
                     MLSSQEPYIAIQNLSLGGDVSLQGSPISLKKLGFGMDKDSLKGFFFTADVNIEKEGES
                     GVSAGLGAAVLGKVTLVEGRQQWSFKKLDIQDGSIAAKFPGFEFRGSIKPFSNEVYGT
                     GFQAELQLKIDFSGNKDAKSESNNQFLVEAFGIFGNKKNPDIKYWLVDVKAEFPCIPI
                     FTGVQLCGIMGGAYRRMTAVGMGTPGDNFELGVLPSGVKYEPDADAGLGIRAGVKIRI
                     TGGNTITGSALFEFAFSESGSLDRVFFQGMVEIQVPIDKIPGVVNQMTKIVDKLKLDK
                     VEYLNMLKSDDKKSAAQTGNNQIKATLTTNLIFEDQYYHSTLEVFLNVANGYLKGFGM
                     ADMLADFKNDKYHFYLGTFDNKISLAISISGFQAQVWTYTMFGNDIAGIPPLDPNVAK
                     ALNISPTQNSADAAAYMGNVATGTGMLFGAGLGLSFENCWRIVFVNVYAKASFDAAFE
                     AGILDFGEGKKCKNGTGPIGKDGWYTFARSYLIIAAEGGRRKNCSKDYNKWVDVAVGV
                     FLEGSFPNPNYILAKGIARFCGFSIKVEVQKGEKCL"
BASE COUNT          530 a          353 c          481 g          519 t
ORIGIN      
        1 tatatcaagc tggtatatgc cgattcaaaa ttctttccga ctgcctgtct gagtggcaag
       61 ctgaccatca tgtctcttaa caacagcggc aaaaaggaac tggaagcgcc caatataatt
      121 tttcaaaacc tgatgttgtc atcccaggag ccgtacatag ccattcagaa tctttctctt
      181 ggcggcgatg tgtctttaca gggttcgcca atatcattga agaaactggg ttttggaatg
      241 gataaagaca gtctgaaggg gtttttcttt acggccgatg tcaatataga aaaggaggga
      301 gagagtggtg ttagtgcagg gttgggggca gctgtactgg ggaaggttac tttggttgag
      361 ggtcgacaac aatggtcatt taagaaactg gatattcagg atggttccat agccgcgaaa
      421 tttcccggat ttgagtttag gggatctatt aagccatttt ccaatgaggt ttatggtacg
      481 ggctttcagg cagagttaca actgaagata gatttttcag ggaataagga tgctaaatcc
      541 gagtcgaaca accaattttt ggtagaggct ttcggaatat ttggcaataa aaagaatccg
      601 gatatcaaat actggcttgt cgatgtcaag gcagagtttc cctgtatccc catctttact
      661 ggcgtacagc tgtgcggtat catgggtggc gcctacagaa ggatgacggc ggtcgggatg
      721 ggcaccccgg gcgataactt tgaattgggt gtgttgccga gcggtgtgaa gtatgagcct
      781 gatgctgatg ccggactggg catcagggca ggagtgaaaa tacgtatcac cggaggtaat
      841 accattacgg gatcggcctt atttgaattt gctttttctg aatccggatc tcttgacagg
      901 gtatttttcc agggaatggt tgagattcag gtgccgatag ataagattcc cggtgtggtc
      961 aatcaaatga ccaaaatcgt agataaatta aaacttgata aggtagaata tcttaatatg
     1021 ctgaaaagcg atgataagaa atccgccgca caaaccggca ataatcagat aaaggctacc
     1081 ctgaccacca acctgatttt tgaggatcaa tattatcaca gtacactgga agtctttctg
     1141 aacgtcgcca atggctacct gaaaggtttt ggaatggctg atatgctggc agattttaaa
     1201 aacgataagt atcattttta cctgggcacc tttgataaca aaattagttt ggcgataagc
     1261 atttcaggct ttcaggcaca ggtttggacg tataccatgt ttggaaatga tatagcgggt
     1321 attcctcccc ttgatcccaa tgtggccaag gctttaaata tatctccgac acagaattca
     1381 gcagatgcag cagcctatat gggcaatgtc gctacaggaa caggtatgct cttcggtgcc
     1441 ggattgggcc tgagttttga aaattgctgg agaatagtct ttgtcaatgt ctatgcgaaa
     1501 gcttcctttg atgcagcctt tgaagccggc atccttgatt ttggcgaagg taaaaaatgt
     1561 aaaaatggaa ctggccctat tggtaaggac ggctggtaca catttgcccg gtcatacctg
     1621 ataattgccg cagagggcgg cagacgaaag aattgcagca aggattacaa caagtgggta
     1681 gacgttgcag tgggtgtatt tttggaaggc tccttcccca atccaaacta tatcctggca
     1741 aaaggaatcg cacgtttttg tggatttagt ataaaagttg aagttcaaaa aggagaaaag
     1801 tgtctatgaa actgaaacgt tatattgtac taatcctcct gacaggtttt gctatcagcg
     1861 tccacagtca acctggcagg att
//
LOCUS       DAOQMC010000349         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_44125_length_1866_cov_4.027609, whole genome shotgun sequence.
ACCESSION   DAOQMC010000349 DAOQMC010000000
VERSION     DAOQMC010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44125_length_1866_cov_4.027609"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PKV31_10195"
     CDS             <1..681
                     /locus_tag="PKV31_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HNB93273.1"
                     /translation="GKSYRIGKFGYDRHNIHGLLFDSEFSIDNIIESDDKVNIVLVTT
                     YRGEDSGFPFLYDLKLDYQVDDSGFICIKTTVKNVGNSGMPVVDGWHPYFSVSSRIDD
                     VSLCILADEVLQFDENLIPTGEKLVFSDFNSPGLIGGMQLDNCFPLNGRTGMVCSLSY
                     EELKLEISAWKHYNYLQLFIPPDRKSIAIELLSGAPDAFNNGMGLTVLQPGESRIFEC
                     SYHLKLSQ"
     gene            complement(721..1182)
                     /locus_tag="PKV31_10200"
     CDS             complement(721..1182)
                     /locus_tag="PKV31_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB93274.1"
                     /translation="MVTIEEKTEISTSEKDFIQRIWNSEFPAVSKLDENKELEFYLKK
                     TTDHRYYFLKVDGKLAGWGFKFKRYSGTWFALALEREYQGKGLGSKLFNEIKKNETEI
                     NCWAIDENIHNKADGSLYRSPFDFLKANGMTPFENIRGHDGKLTAIKFKSS"
     gene            complement(1213..>1866)
                     /gene="dapA"
                     /locus_tag="PKV31_10205"
     CDS             complement(1213..>1866)
                     /gene="dapA"
                     /locus_tag="PKV31_10205"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNB93275.1"
                     /translation="NNTAALTQRIVSTDLSGVDAIVSSSPAYVKPTQEGIYQHYMRIA
                     DSSAKPVIIYNVPSRTAAKVSHETILRLAESHDNFLGVKEATADFFALSKLIKHRPDN
                     FLVWSGDDYTAFPTMALGADGGISVIANAFPREFSEMIRACLKGDFARGAELHMQMLD
                     LHPILYAEGNPTGIKGAMEDLGLCSKEVRLPLTPLSEAANRQLIHEIKKAGLAGVKE"
BASE COUNT          468 a          349 c          425 g          624 t
ORIGIN      
        1 ggcaagagtt atcggattgg gaagtttggc tatgacaggc ataatattca tggcttatta
       61 tttgacagcg aattttcgat cgataatatc attgagtcgg atgacaaggt gaatattgta
      121 cttgtcacca catacagagg tgaagatagt ggtttccctt ttctatatga tttgaagttg
      181 gattaccagg tggatgattc gggattcatc tgtattaaga caacagtaaa gaatgttgga
      241 aattctggta tgcctgtggt cgatggctgg catccttatt tttctgtttc atcccggatt
      301 gatgatgtga gtctttgtat cttagcagat gaggtattgc aatttgatga aaacctgata
      361 cctacgggag aaaagttggt tttctctgat tttaattcac ccggattgat tggcggtatg
      421 cagttggata attgttttcc attgaatggt aggaccggca tggtgtgtag cctttcgtat
      481 gaggagttaa agttggagat atctgcgtgg aaacattata attacctgca attattcatt
      541 cctcccgaca gaaaaagtat agccattgag ttgctgagtg gggctccgga tgcattcaat
      601 aatggaatgg gtttgacagt tcttcaaccg ggagaatcga ggatatttga atgttcttac
      661 catctcaaat tatcgcagta gtccagaatt ttatccaatt tttattctta ttagcaaaag
      721 tcaggatgat ttaaacttga ttgcagttag ctttccatca tgaccacgaa tattctcaaa
      781 cggcgtcatt ccattggctt ttaagaagtc gaagggcgag cgatatagtg atccgtcagc
      841 tttattgtgt atgttttcat ctatagccca acaattaatt tcagtttcat tttttttgat
      901 ttcattaaat agttttgagc ctagtccctt gccctgatat tctctttcta aggccagtgc
      961 aaaccaggtt ccggagtaac gtttgaattt gaaaccccat ccggccagtt tcccatccac
     1021 tttcagaaaa taatatcgat ggtcggtggt tttcttcagg tagaattcga gttctttgtt
     1081 ttcgtctaat ttactaaccg ccggaaattc gctgttccag attctttgga taaagtcttt
     1141 ttccgaggta gatatttcgg ttttttcttc gattgttacc atgagacacc gaattacctt
     1201 aaaaaataat atttattctt ttacaccggc tagtcctgct ttcttgattt catgtattaa
     1261 ctgtcggtta gctgcttcac taaggggtgt cagagggagt cttacttctt tggagcaaag
     1321 tccaaggtct tccatggcgc cttttattcc tgtcgggttg ccttctgcat aaagaatcgg
     1381 atgtaagtcg agcatttgca tgtgtagttc agcgcctctg gcaaaatctc ctttcaagca
     1441 tgctctgatc atttcggaaa attcccgtgg aaaggcattt gcgataaccg atatacctcc
     1501 gtcggctccc agtgccatgg tggggaatgc tgtataatcg tcgcctgacc agactaaaaa
     1561 gttgtcaggt cggtgtttta tcaatttgct caaggcaaaa aaatcagcgg ttgcttcctt
     1621 aactcctaaa aaattgtcgt gtgattctgc cagacgtaat atggtttcgt gtgatacctt
     1681 tgcagcggta cggcttggta cattgtatat gataacaggt ttggcacttg agtcggctat
     1741 gcgcatataa tgctgataga tgccttcctg ggtcggcttg acgtaagcag gactgctgga
     1801 cacaattgca tcgactccgg acagatccgt ggaaacgatg cgttgtgtca gggcagctgt
     1861 attgtt
//
LOCUS       DAOQMC010000350         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_44431_length_1859_cov_3.223947, whole genome shotgun sequence.
ACCESSION   DAOQMC010000350 DAOQMC010000000
VERSION     DAOQMC010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44431_length_1859_cov_3.223947"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1730)
                     /locus_tag="PKV31_10210"
     CDS             complement(192..1730)
                     /locus_tag="PKV31_10210"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNB93276.1"
                     /translation="MMKNINRLYQAGILVLTLILSTGCSKDFLEVPIQGQATPDSDPA
                     IAEKLVTGVYNSMLAGSAFGGIGDVHGISFITATIIMTDDAEKGSTASDQPNIAAIDN
                     FEHTSTNNFVAALWTGNYNAIAKANQALKALDVAPIADAIKTQYQGEVRFLRAYFYFN
                     LVRYFGGVPKVLQIPADANEANSNPVYQTRASVDEIYSLIREDLNYAIEHLKPRSQSQ
                     VGRANKGAAQAMLAKVELTTRNWQNAYNLCQEVINSGQYSLLAKYEDIWRQVGDNSVE
                     SIFEVQTGQFNNSDFGVEGYCVWQGPRVGGKGGWRDLGFGFNNPTVDLVNEYEPGDIR
                     KDATIIFIDNSGTHKGTILFDGYRIPSQDSVQNARYNYKAYHSENKEIESFFGNRDRK
                     AKNIHLLRYGDLLLMAAESANELGNTGEAATYLNMIRERATLSATTASSQADMREAIY
                     HERRVEMAMEHDRWFDLVRTGRAAKVMKPLKPSFTEGKNELLPIPALQIALSGGKLLQ
                     NPGY"
BASE COUNT          502 a          440 c          362 g          555 t
ORIGIN      
        1 tggctacttc atcggcattg ttgtaaccgt caatcggggg taatttgttg tcatcgtctt
       61 tacaagaagt gaaactgatt gctacaattg ccaatacaaa caaggcaatt tgcttaaaat
      121 ttttaataac cattgtttta aaattttgat aaatttataa tataattaaa taaattctaa
      181 agacacaggc atcaataccc gggattttgc aataattttc ctccactcaa ggcaatctgt
      241 agtgccggaa tcggcaacaa ttcattcttg ccttcagtaa aggaaggctt caacggcttc
      301 ataaccttgg ctgcacgccc agtcctgacc agatcaaacc aacggtcatg ctccatcgcc
      361 atctctacac gtctttcatg atagattgct tcgcgcatat cggcttgaga agaggctgta
      421 gtcgcggaaa gggttgctct ttcccggatc atattgagat aagtcgctgc ttctcccgta
      481 ttgcccaatt cattagcaga ttcagcggcc ataagcaata ggtctccata cctcagcaag
      541 tggatattct tggcttttct atcacggttg ccgaagaagg attctatctc cttattctct
      601 gaatgatagg ctttataatt gtaccgtgca ttttggacgc tatcctgact tgggatgcga
      661 tagccatcaa aaagtattgt tcccttatga gttccgctat tatcaataaa tatgatggtt
      721 gcatcctttc gtatatcacc gggttcatat tcattgacca gatctaccgt aggattattg
      781 aatccaaagc caagatctct ccaaccaccc ttacctccga ctcttggccc ctgccaaaca
      841 caatacccct ctactccaaa gtcagaatta ttaaactggc ctgtctgcac ttcaaaaata
      901 gattccacac tattgtcgcc aacctgtcgc cagatatctt catactttgc caacaagctg
      961 tattgtccgg aattgattac ttcctgacat aaattatagg cattttgcca attcctggta
     1021 gtcagttcga ctttggccaa catggcttga gctgcgccct tattggcccg tccaacctga
     1081 gactgagacc ttggtttcag atgttcaatg gcgtagttca ggtcttcacg aatcaggctg
     1141 tatatctcat caacacttgc acgggtttga tacacaggat tcgaatttgc ctcattggca
     1201 tctgccggaa tctgcaaaac tttcgggaca cctccgaaat accgtaccag attgaagtaa
     1261 aaataagctc tcagaaaccg gacttctccc tgatattgcg ttttaattgc atcagcgatg
     1321 ggagcaacat ccagagcctt tagcgcttga ttagccttgg caatcgcatt ataatttccc
     1381 gtccatagtg ccgccacaaa attattggtg gaggtatgtt caaaattgtc tatggccgcg
     1441 atattaggct gatcggaggc agtacttccc ttttcagcat catcagtcat aataattgtg
     1501 gcagtaatga aactgattcc gtggacatct ccaattccac cgaatgcact tcctgcaagc
     1561 atggaattat aaactcccgt aaccaatttt tctgctatgg caggatctga gtccggtgta
     1621 gcctggcctt gtatcgggac ttccaaaaaa tctttgctgc acccggtcga aaggataagg
     1681 gtcagcacca atattccagc ttgatatagt ctgttaatat ttttcatcat ggctttcaac
     1741 attagaattc attaaaaagt gaaattgatg cccgctgcaa tggttcggga tgtcggataa
     1801 gcattcagtt caattcctga gctggttgga tcacccggaa gttctgcagt aaaaccact
//
LOCUS       DAOQMC010000351         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_44916_length_1849_cov_4.631550, whole genome shotgun sequence.
ACCESSION   DAOQMC010000351 DAOQMC010000000
VERSION     DAOQMC010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44916_length_1849_cov_4.631550"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1493
                     /gene="glmM"
                     /locus_tag="PKV31_10215"
     CDS             114..1493
                     /gene="glmM"
                     /locus_tag="PKV31_10215"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNB93277.1"
                     /translation="MTLIKSISGMRGTIGGHPGENLTPVDIVEMAASFGQMIKKNNSH
                     PSIVIGRDGRISGNMVSQLCIHTLVAQGFIVYDAGLSTTPTIEMAVTKLGADAGIIIT
                     ASHNPIQWNALKFLNEKGEFISAAQGQELIRIAHEKQYLFNEIHNLGKIIPAAGLIEH
                     HIDSILNLPLVNKNIVADKKFKVVVDCINSTGAISVAPLLEALGCEVILINEEMTGNF
                     AHNPEPLAQHLQELCERVISEKAEMGIAVDPDVDRLSFICEDGSLLGEENTLVSVANY
                     ILQHTPGETVSNLSSTRGLKDITLRYGMEYHAATVGEVNVVEKMKEVNAIIGGEGNGG
                     IIYPALHYGRDALVGIALMLTYMAENNLSLTQIKASLPQYFISKNKIDLQPGWNVTAA
                     INGLIDKYSEEEINTSDGLKIDFAKGWVQLRQSNTEPIIRVYSESYDEATANRLAEKI
                     IGEFKELIG"
     gene            1588..>1849
                     /locus_tag="PKV31_10220"
     CDS             1588..>1849
                     /locus_tag="PKV31_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB93278.1"
                     /translation="MDRIYLDNAATTPLDPEVLDIMCKTLQNDFGNPSSTHSEGRKAR
                     ALIEAARKSIASCIHASPSEIIFTSGGTESNNMALKCAVRDLH"
BASE COUNT          565 a          384 c          419 g          481 t
ORIGIN      
        1 tggggatggc ccacaatctc tttattacat agacaaggaa ttggaggcca tcaataaccg
       61 tcagttggct gcggaaggaa aggcctgatt gttttactct taagaacaaa cttttgactc
      121 taatcaaatc tatatcaggg atgcgtggta cgataggcgg gcatcccgga gaaaacctca
      181 ccccggttga tatcgtggag atggccgcat cgtttggcca aatgatcaaa aagaacaatt
      241 cacacccttc aatagtaatt ggcagggatg gcaggatatc cggcaatatg gtatcccaac
      301 tatgcataca caccttagtg gcgcaaggtt ttattgtata tgatgcagga ctttccacca
      361 cgccgacgat agaaatggca gttacaaagc tgggagctga tgccggaatt attatcacag
      421 caagccacaa tcccattcag tggaatgctt tgaaattctt gaacgaaaag ggagaattca
      481 tatcagccgc tcaaggtcag gaattgatcc ggattgctca tgaaaaacag tatctattta
      541 acgaaattca caatctaggt aagatcattc ctgctgcagg cttgatagag catcatattg
      601 attccattct gaatcttcca ttggtcaata aaaatatagt tgcagacaaa aaatttaaag
      661 tcgtggtcga ttgtatcaat tcgaccggag cgatttcagt tgcaccactg ctggaggctt
      721 tgggttgcga ggtcatccta attaatgaag agatgacagg caactttgcc cacaatccag
      781 agcctttggc acaacacctg caggaattgt gtgagagggt gatatcagaa aaagccgaaa
      841 tgggtatcgc cgtggatcct gatgtggata gattaagctt tatatgtgaa gatggcagcc
      901 ttttgggaga agaaaatact ttggtatcag ttgcaaacta catactccaa catacgcccg
      961 gtgaaacggt ttccaacctt tcgtcaacca gagggctgaa ggatattacc cttagatatg
     1021 gaatggaata tcatgctgct actgtcggag aggttaatgt ggtagagaaa atgaaggaag
     1081 ttaatgccat tatcggtgga gaaggaaatg gcggcatcat ttatcctgcc ctgcactatg
     1141 ggcgtgatgc actcgtggga atagcgctca tgctcaccta tatggcggaa aataatctca
     1201 gtttaactca gataaaagct tctttgcccc aatattttat ttcaaaaaat aaaattgatc
     1261 tgcaaccggg ctggaatgtc acggcagcaa tcaatggctt gattgacaaa tattcggagg
     1321 aagaaatcaa tacctcagat ggcttgaaaa tagactttgc caaaggctgg gttcaacttc
     1381 ggcagtctaa tactgagcct attattcggg tttatagcga atcttatgat gaagctaccg
     1441 caaatagact ggcagagaaa atcattggtg agttcaagga attgattgga tgacatccgg
     1501 gatcctcaaa aacatcattg atcctaactt catgttactt aaccggttat aaaaatgaca
     1561 atctttcatt tcaaacaatt gtaatccatg gacagaatat atcttgacaa cgcagccacc
     1621 accccactcg accctgaggt gctcgacata atgtgtaaga cattgcaaaa cgacttcggc
     1681 aatccctcaa gtacacattc agagggccgt aaggcaaggg ccttgatcga ggcggcccgc
     1741 aaatctatcg cttcctgtat ccatgcttct ccaagtgaaa tcatctttac ctcaggcggt
     1801 actgaatcca acaatatggc tttgaagtgt gcagttcgag accttcatg
//
LOCUS       DAOQMC010000352         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_45140_length_1844_cov_4.697596, whole genome shotgun sequence.
ACCESSION   DAOQMC010000352 DAOQMC010000000
VERSION     DAOQMC010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45140_length_1844_cov_4.697596"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PKV31_10225"
     CDS             complement(<1..940)
                     /locus_tag="PKV31_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93279.1"
                     /translation="MRYLQLVLVIFIPAIIFSQPINDDCINAIEITDVANYCSAFGEF
                     NNFGATPSIPSDYICIPNEATARDVWFKFTATANFIHLTVNGKSLGNPGGTLVSPQVI
                     LFSGPCNALVEQDCFSDAFGINSLEVFEGPLVPGQTYYINISARDNHLGTFQLCLNNY
                     NQNFVPDGDCPTSRVLCDKSSVFIEAVFNPGNITDEIPLFSCVREEIASSWFRWTCSK
                     SGTLTIKLTPTKPEDDLDFALFELPNGIDDCNGKKQLRCGAAGENVGEPYNNWKQCTG
                     PTGLNLTSTDINEDPGCNNGNDSWVKYIDMVEGVSYA"
     gene            1047..1760
                     /gene="pssA"
                     /locus_tag="PKV31_10230"
     CDS             1047..1760
                     /gene="pssA"
                     /locus_tag="PKV31_10230"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNB93280.1"
                     /translation="MIRHIPNILTLLNLFCGALAVISVLFLHVEEALVLQAISLIADF
                     LDGLIARKLNVAGPLGVQLDSLADVISFGIFPGAVVYMLLVQSWDIDPGMSDLESRLL
                     LYPAFLLPVFGALRLARFNISTDQGDKFIGMPIPSMSIFFAGLLMIQLNGDIRMIEFI
                     ENKWFLYGSILLFAYLMNSPWIHFKIKPDKKTFSNPFILLLAGAIIGLIIFYPFLAPT
                     IGIVLYSTFSIISHLLKLS"
BASE COUNT          527 a          390 c          357 g          570 t
ORIGIN      
        1 aggcataact aacgccctca accatatcga tgtatttcac ccaactatca tttccattgt
       61 tacatcccgg atcctcattg atatcggtac tggtcaaatt taatccggta ggtccggtac
      121 attgtttcca attattataa ggctctccga cattttctcc tgccgccccg caacgcagct
      181 gctttttacc attacagtca tctatgccgt tgggaagttc aaacaaggca aaatcaagat
      241 cgtcttcagg tttagtcggt gtgagtttga tggtcagtgt accagactta ctacaagtcc
      301 atctgaacca ggaagaagca atttcctccc ttacacaaga aaaaagcgga atttcatcgg
      361 ttatatttcc gggattaaac acagcttcaa tgaatacgga agatttatcg caaagaactc
      421 tacttgtcgg gcaatcgcca tctgggacaa aattttgatt ataattgttc aaacataatt
      481 gaaaagtccc caaatgatta tcccgggcac tgatgttgat ataataggtt tgacccggaa
      541 ccaaaggtcc ttcaaagact tcaagtgaat tgatcccaaa tgcatcgctg aaacaatcct
      601 gttctaccaa tgcgttgcaa ggaccggaaa ataggatcac ctgcggtgaa actaaagtcc
      661 caccgggatt tccaagggat ttaccattaa cggttaggtg gataaaattt gctgttgctg
      721 taaatttaaa ccaaacatcc cttgcagtag cttcattggg aatgcatata taatccgaag
      781 gaatggaagg cgtcgcccca aaattattga attccccaaa agccgagcaa taattagcca
      841 catccgttat ctcaattgca ttgatacaat catcattaat gggttgagaa aaaattattg
      901 caggaatgaa gatgaccaaa acgagttgca aatatctcat gctaattgaa ttgaaaacta
      961 tactgtgtta tgcaataaac gaataattaa ccacttttgt tagatttacc ctttaaattt
     1021 gcatttttcc aactaagaaa cagacaatga tcaggcatat tcccaatatt ttgaccttgc
     1081 taaatctttt ttgtggagca ttggcagtca taagtgtttt atttttacat gttgaggaag
     1141 cattggttct tcaggcaatc agcctgatcg ctgatttcct tgacgggctt attgccagaa
     1201 agctaaatgt ggccggtcct ttgggtgtac aattagactc tttggccgat gtcattagtt
     1261 ttggcatatt cccgggtgca gtcgtatata tgctgttggt tcaatcctgg gatattgacc
     1321 ctggtatgag tgacctggaa agccggcttc tactctaccc tgcttttctg ctgcctgtct
     1381 ttggagcatt aaggttagcg cgattcaata tctcaacaga ccagggtgat aaatttattg
     1441 gcatgcccat cccgtccatg tccatatttt ttgcaggatt gctcatgatt caattaaatg
     1501 gggatattcg aatgatagaa tttatagaaa acaaatggtt cctctatgga agtatcctgt
     1561 tgtttgccta tttgatgaac agtccatgga ttcatttcaa gattaagccg gataagaaga
     1621 cattcagcaa ccctttcatt ttattgctgg cgggcgctat catcggattg atcattttct
     1681 atcccttcct agccccaaca ataggaatcg tactttattc cacattttcc attatttccc
     1741 atctccttaa actttcataa tggaaaagga gcccggtaga ctttatgttg ttcccactcc
     1801 tgtgggtaat ttgggagatt ttaccttcag ggccattgag actc
//
LOCUS       DAOQMC010000353         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_45815_length_1830_cov_2.686761, whole genome shotgun sequence.
ACCESSION   DAOQMC010000353 DAOQMC010000000
VERSION     DAOQMC010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45815_length_1830_cov_2.686761"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /gene="ccoG"
                     /locus_tag="PKV31_10235"
     CDS             <1..1198
                     /gene="ccoG"
                     /locus_tag="PKV31_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNB93281.1"
                     /translation="VLEGKFILFSKIFWPQDFYIFAIAMITFIIFIALFTVVYGRLFC
                     GWVCPQTIFMEMVFRPLEWLIEGNPAAQKQLNNGPWNFTKVWKKSLKHFIYFVISFLI
                     AHTFLSYILGVDKVIKIIEEPLSEHVAMFIGLIAFAFAFYGVFAYVREIVCTTICPYG
                     RLQGVMFDKDTMLVAYDYKRGEDRAKFKKNEQRTAGDCIDCHQCVNVCPTGIDIRNGT
                     QLDCVGCTACIDACDFMMDKVGLPKGLIRYASENGIAEGKKLTFTPRIKAYSVLLVIL
                     LTILTVLMITRNDVDTHITRTGGQLYQELPDHKLSNLYNAKIINKTNSEFPVELKLEN
                     AKGEIKLVGKQVLSLKKESIHTETFFIILDKTEIHDRTTKLKVGVYKDGKKIQTVKTN
                     FLGPFM"
     gene            1237..1674
                     /locus_tag="PKV31_10240"
     CDS             1237..1674
                     /locus_tag="PKV31_10240"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNB93282.1"
                     /translation="MSWGYRIMIVYIVFIAMILGMVFVASKQTNEMQDENYYSKELVY
                     QSVIDGKNNLNALADSVTLSNQPGSVKIKLPLAASANITEGSVYFLCPSDEKKDLKDK
                     LNVDANGEQSISRDKLKDGFYTIQISWKSNGKAYYSEQKFKVQ"
BASE COUNT          552 a          328 c          429 g          521 t
ORIGIN      
        1 tgtgctggaa ggtaagttta tcctgttcag caagatcttc tggccgcaag acttctacat
       61 cttcgccatc gcgatgatca cgtttatcat cttcatagcc ttgtttaccg ttgtgtatgg
      121 caggttgttt tgcggatggg tctgccctca gaccatcttc atggagatgg tgtttcgtcc
      181 gttggagtgg ctgatagaag gaaatccggc tgcacaaaaa caactgaata acggaccatg
      241 gaattttaca aaggtctgga agaaatcact gaagcatttc atctatttcg tgatttcatt
      301 cctgattgcc catacgttcc tgtcttatat attgggtgta gacaaggtaa tcaagatcat
      361 tgaggagcct ttgtctgagc atgtagcgat gtttatcggg ctgattgcct tcgctttcgc
      421 attctatggc gtatttgctt atgttcgtga aatcgtatgt acaaccattt gcccttacgg
      481 aagacttcag ggggtgatgt ttgataaaga taccatgctg gtggcctatg attataagag
      541 aggagaggat agagcgaaat tcaagaagaa cgaacaaaga accgcagggg attgcattga
      601 ctgtcatcag tgtgtgaatg tctgtcctac cggaatcgac atccgtaacg gtacacagct
      661 ggattgtgtg ggttgtaccg cctgtattga tgcatgtgat ttcatgatgg ataaggtagg
      721 attgccgaaa ggtctgatcc gttatgcatc tgaaaatggc attgcggagg gtaagaaact
      781 gactttcacg cccagaatca aggcgtattc cgttttactg gtcatcctat tgaccatatt
      841 gacggtattg atgattacca gaaacgatgt ggatacccat attacccgta ccggaggaca
      901 attgtatcag gagttgccag accataagct tagcaatctc tataatgcca agatcatcaa
      961 caagacgaac agcgaatttc cggtggaatt gaaattggag aatgccaaag gtgaaataaa
     1021 actcgttggt aaacaagtgc tgagtctgaa aaaagaaagc atccatacag aaacgttctt
     1081 tatcattctg gacaaaactg agattcacga cagaacgacc aaactaaaag tgggtgtcta
     1141 caaggatggt aaaaagatac agacggtgaa aacaaatttc ctcggaccat ttatgtaaga
     1201 aaacttattt ttacaaacac aaaaaagcaa ggttttatga gttggggata tagaataatg
     1261 atcgtataca tcgtatttat tgccatgata ctcggaatgg tctttgtagc atcgaaacag
     1321 accaatgaga tgcaggatga aaattattac tcgaaagaac tggtttatca atcggtgata
     1381 gatggtaaaa acaacctgaa tgctcttgca gattctgtta cgctgagcaa ccagccggga
     1441 agtgtgaaaa taaaactgcc tttggctgca tctgccaata taacagaggg ttccgtctat
     1501 tttctttgtc cttccgatga gaaaaaggac ttgaaagata agttgaatgt agatgcgaat
     1561 ggagaacaat ccatttcaag ggataagctg aaagatggat tctataccat ccagatcagc
     1621 tggaagagca acggaaaagc ttattattcc gagcaaaaat tcaaggtgca atagatagaa
     1681 tgatttggca attcatcata gcaggtttta ccattggtat tttaggtagt tttcattgta
     1741 ttggtatgtg tggaccaatc gcactcgcat tgcctgtcgc agatgccaca aagtggaagc
     1801 gtgttttttt tatcttattg tataatgtag
//
LOCUS       DAOQMC010000354         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_46082_length_1823_cov_4.486991, whole genome shotgun sequence.
ACCESSION   DAOQMC010000354 DAOQMC010000000
VERSION     DAOQMC010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46082_length_1823_cov_4.486991"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>1823
                     /locus_tag="PKV31_10245"
     CDS             31..>1823
                     /locus_tag="PKV31_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93283.1"
                     /translation="MDKGIGETQPPKSTNKISPLFRSYWGNETGVRTFPAINYIVNNT
                     NNIWEVLPGVKRDLNFILTVKENKAGGANHVQAKNKLAVDNNSGPFLVTYPNQKYITW
                     FVGEQKTITWDVSNTDKSPVNCKNVDILISEDGGYTYPHLLGTFPNIGHADITVPDIE
                     GQKIRVKIAASDNVFFDISNEDMVIRRGLPPFGISMDVNPEILCKGDSLEFYVRAISP
                     DTLTDTVSVSINNPNTGLKVSFDSEKFLDNDSIRVTIYNEGAATKANVIEFFFTANGH
                     TIFRQVQIQTYSAPSAPTLYNPVNFSENVTPGLKFVWNAVTGQPVTYDLEVATDIDFF
                     NIVAKRVDVELPQAVLAENLADNTVYFWRVKANGVCESSPYSGTYVIRTGQCNAFYST
                     PGKVIELSGSIVSDTITVDSLPFEKLQSLEVIGLKGHTSNIGSIKASLVNADQKSSIL
                     WQSSCSGEKEMRLRFSDNAYSESIPCDTLDQVFGLGDLSVKPVESLSRFNSSTAENTY
                     ALVLSMEDSTTTGQMQQWGLNLCGSAPTCKPLRIPTTSRLYTADLACTDDEGWTHYSI
                     TAANNSGSNVDLMVMSIKYNGADPLPADKISV"
BASE COUNT          556 a          346 c          397 g          524 t
ORIGIN      
        1 gattcatctt tgatctatca atgggaccaa ttggataaag gaattggaga aacccagcca
       61 cctaaatcta ctaataagat cagtcctctg ttcagatctt actggggtaa tgagacgggt
      121 gtaaggactt tcccggcaat caattacatt gtcaataata caaataatat atgggaggtg
      181 cttcccggtg tcaaacggga tttgaatttt attcttactg tcaaggagaa taaggcaggt
      241 ggggctaatc acgtgcaagc caagaacaaa cttgcagttg ataataacag tggtcctttt
      301 ctagtaacct atcccaatca aaaatatatt acttggtttg tcggagagca aaaaaccata
      361 acctgggatg tttcaaatac agataaaagt cctgtcaact gtaaaaatgt ggacatattg
      421 atctcagaag atggtggcta tacatatcct cacctgcttg gcacttttcc aaacatcgga
      481 cacgctgata taacggttcc tgatattgaa ggtcagaaaa tcagggttaa gattgccgca
      541 tctgataatg tgttttttga tatatccaat gaagatatgg tgataaggag aggattaccg
      601 cctttcggaa taagtatgga tgtcaatccg gagattttat gtaagggtga cagtctggag
      661 ttttacgtga gggctatttc acctgacaca ttaacggata ctgtgagtgt tagcatcaat
      721 aatcccaata caggattgaa agtgagtttt gattccgaaa aatttttgga caatgattcc
      781 atccgggtta caatttataa tgaaggagcg gccacaaagg ccaatgtgat cgaatttttc
      841 tttacagcga atggtcatac cattttccgg caagttcaaa ttcagactta ttcagcgccg
      901 tcagctccta ccctatacaa tcctgtcaat ttcagtgaga atgtcacacc cggccttaaa
      961 ttcgtctgga atgcagttac cgggcaacct gtaacttatg acctggaggt tgccacagat
     1021 atagatttct tcaatattgt cgcaaaaaga gttgatgttg aattgcctca agccgtatta
     1081 gctgaaaatc ttgcagacaa tactgtttat ttctggagag taaaagcaaa tggtgtatgt
     1141 gagagctcac cttattccgg aacatatgtg attcgtacag gccagtgcaa tgcattctat
     1201 tcgacaccag ggaaggtgat cgaacttagt gggtcaattg tgtcagatac gattacggtt
     1261 gacagtcttc cgtttgagaa gcttcaaagc cttgaagtca ttggattgaa agggcatact
     1321 tcgaatatag gttcaatcaa agccagtctc gtgaatgctg atcaaaaatc ttccatttta
     1381 tggcaaagca gctgttcagg cgaaaaagag atgaggttga ggttttcgga taatgcttat
     1441 tcagagtcca ttccatgtga tacgcttgat caagtgtttg gattaggtga tctttcagtc
     1501 aaacctgtcg agtccttatc aagatttaat tcatcaacag cggaaaacac ctatgcgctc
     1561 gtccttagta tggaagattc aaccactaca ggacaaatgc aacaatgggg actaaatctg
     1621 tgtggaagtg caccaacttg taaaccattg aggatcccaa ctacatctcg tctttataca
     1681 gctgatcttg cctgtacaga tgatgagggt tggacacact atagtattac cgcagccaac
     1741 aattcaggca gtaatgtaga ccttatggtt atgtccatca aatataacgg agcagatcca
     1801 ttgccggctg ataaaattag tgt
//
LOCUS       DAOQMC010000355         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_46725_length_1811_cov_3.125854, whole genome shotgun sequence.
ACCESSION   DAOQMC010000355 DAOQMC010000000
VERSION     DAOQMC010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46725_length_1811_cov_3.125854"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKV31_10250"
     CDS             complement(<1..730)
                     /locus_tag="PKV31_10250"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB93284.1"
                     /translation="MTLEKNENGELDVRVLIPIQRHELLVGLFKDLPTHENFVFINDH
                     DPIPLYYEFKSIYGDVVGWEYLNRGGREWKVRVTRTEDSQARDMSGIATLLDLRNADK
                     KEWKQVVFHRYGMMEEGTTMELIAREDLQEIHGIFKSKFAGMHTWMYKKEVPDEYVIH
                     IRKEAKSGLGDDGYSVVNEFDVRPYPPTERHEMFYKAFGDIKPGEAFEFVNDHDPKPL
                     YYQMEAESKEPFRWEYLEKGPEVWK"
     gene            complement(774..1202)
                     /locus_tag="PKV31_10255"
     CDS             complement(774..1202)
                     /locus_tag="PKV31_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB93285.1"
                     /translation="MLSNTSKTAIKAVIYLAGKSECTKASIREVADHINASEHTVGKV
                     LQTLARHKIITSLKGPSGGFFLSDQQKFQPISVIIESIEGKGVFKECGLGLSKCSEIH
                     PCPIHDEYKIARGLMERIFREKMIKDLHDPVTDGLAFLMD"
     gene            complement(1205..1534)
                     /locus_tag="PKV31_10260"
     CDS             complement(1205..1534)
                     /locus_tag="PKV31_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNB93286.1"
                     /translation="MSVNIETNNPEKCDYALIGLYEVYDPEVGLNVVDLGLIYELDFD
                     EDNKSINCRMTLTTQFCPMGESITSDIMQSLTDAFGDYQINVNLTFNPPWSFEKISPE
                     GRAFLGR"
     gene            complement(1531..>1811)
                     /locus_tag="PKV31_10265"
     CDS             complement(1531..>1811)
                     /locus_tag="PKV31_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93287.1"
                     /translation="GMTFKTLPFIVWNKVYHKKAYTGKAPLPKDLFSEKMYHYMLMAY
                     ILGFGVFVIGIILLNQWLLKIGAAALLMTAILYVLNTTKILIHKPRKI"
BASE COUNT          501 a          444 c          306 g          560 t
ORIGIN      
        1 ctttccatac ttcagggccc ttctccagat attcccatct gaaaggctcc ttactctccg
       61 cttccatctg ataataaagc ggcttcggat catgatcatt gacgaactca aatgcttcac
      121 cgggcttgat atccccaaaa gctttgtaga acatctcatg cctttcagtt ggtgggtatg
      181 gcctgacatc gaattcgtta acaacagagt aaccgtcgtc acctaaacca ctcttcgctt
      241 ccttccggat atggatgacg tattcatccg gcacctcctt cttatacatc catgtgtgca
      301 ttccggcaaa tttgcttttg aatataccat gaatctcttg aagatcttcc ctggcaatca
      361 attccatcgt agtcccctcc tccatcattc cgtaccggtg aaaaacaacc tgcttccatt
      421 ccttcttatc tgcatttcgc agatcgagta acgttgctat tccactcata tcacgtgcct
      481 gcgagtcttc ggttcgggtc acccggacct tccattccct acctccccga ttgagatatt
      541 cccacccaac aacatctcca tatatcgact tgaactcata atacagtgga attggatcat
      601 ggtcattgat aaagacaaag ttttcatggg taggtagatc cttgaacaat ccgaccaata
      661 attcatgtct ctgaatcggg atcaaaacac gtacatccag ttcaccattc tcatttttct
      721 ctaaagtcat actaaattta tatttataat accttttatt acaatacctt atatcaatcc
      781 attaaaaatg caagaccatc tgtgacagga tcatgcaggt ccttaatcat tttttcccgg
      841 aaaatccttt ccatcaggcc tctggcaatt ttatactcgt cgtgaattgg acagggatgt
      901 atctcggaac atttgctaag cccaagccca cattctttga aaacaccttt tccttcaata
      961 gactcaataa ttacggagat aggctggaat ttttgttgat cactcaggaa aaaacccccg
     1021 gaaggccctt tcagactggt aattatctta tgcctggcaa gtgtctgtaa aactttgccc
     1081 actgtatgtt cactcgcgtt gatatgatca gccacctcgc gtatactcgc cttggtacac
     1141 tcacttttac cggcaagata aatgacggct ttgattgctg ttttactcgt attacttaac
     1201 ataattacct tcctaaaaaa gctctacctt ccggcgatat tttttcaaaa ctccagggtg
     1261 gattgaaggt gaggttaaca ttgatttgat aatcgccgaa ggcatccgtc agtgattgca
     1321 tgatatcact ggtgatgctt tcacccatag gacaaaactg tgttgtcaat gtcatccgac
     1381 agttgatact tttgttatcc tcatcaaaat ccaattcata gatgagacca aggtccacca
     1441 catttaagcc tacttcggga tcatagacct cataaaggcc gatcaatgcg taatcgcatt
     1501 tctcaggatt gttggtttca atatttacac tcatatctta cggggtttat gaattaaaat
     1561 tttagttgtg ttgagcacat acagaatcgc agtcatcaat aatgcagcag ctccgatctt
     1621 cagtaaccat tgattcaaaa ggatgatgcc aatcacaaac actccaaagc ccaatatata
     1681 ggccataagc atataatgat acatcttctc tgaaaataaa tcttttggca gaggcgcttt
     1741 ccccgtatat gcctttttgt ggtatacttt attccagaca atgaatggca aggttttaaa
     1801 tgtcatgcct a
//
LOCUS       DAOQMC010000356         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_47552_length_1794_cov_4.359977, whole genome shotgun sequence.
ACCESSION   DAOQMC010000356 DAOQMC010000000
VERSION     DAOQMC010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47552_length_1794_cov_4.359977"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKV31_10270"
     CDS             <1..969
                     /locus_tag="PKV31_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB93288.1"
                     /translation="CYGISDNGKPHLRIENRVIPSGPTVLDEVANAAFWLGAMTGMDD
                     QYKDITKHISWEDVRDNFGKAAQFGIDTKFTWLKDKKITPAELALTELIPLAEHGLKL
                     KGVNDADITRYMDVIRGRADKLMTGARWQLRAFTSLIKHVTRDEAMSVLTQSIIKNQE
                     QEIPIHKWKAPKIKDLEEYRPGTLKVEEFMETDLFTVQKDDIVELVAEMMDWRKIRYM
                     PVEDKKGNLIGLVTSRLLLRHFSNRKVDKKKDVIIVNDIMIKDPITISPDATVLDALE
                     VMRNKQIGCLPVVDKNELVGIITETDFLRITGRLIDRMEHVEEDGE"
     gene            1394..>1794
                     /locus_tag="PKV31_10275"
     CDS             1394..>1794
                     /locus_tag="PKV31_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB93289.1"
                     /translation="MTFEIESNLVDIPQKSIYPVRLRVENGSIAHIMRIDRQVTGYIL
                     PGFVDAHVHIESSLLVPSEFARMAVIHGTVATVSDPHEIANVCGVPGIQYMLDDADGV
                     PFYFNFGAPSCVPATVFETAGDIIGPEEIKA"
BASE COUNT          513 a          339 c          434 g          508 t
ORIGIN      
        1 tgttatggca taagtgataa tggcaagcca caccttagaa tcgaaaacag ggtgattcct
       61 tccggaccta ccgttctgga cgaagttgcc aatgcagctt tttggctggg agccatgaca
      121 ggtatggatg atcagtataa ggacatcact aaacacatat cctgggagga tgtacgcgac
      181 aactttggta aagcagctca attcggtatc gatacgaagt ttacgtggct gaaagacaag
      241 aaaattacgc ctgccgaact cgctttgaca gagttgattc ctttagcgga gcatggtctg
      301 aagctcaagg gggtcaatga tgcggatata acccgctaca tggacgttat aagaggtagg
      361 gccgacaagc tgatgaccgg agcacggtgg cagctcagag cctttacttc actgatcaag
      421 catgtgacaa gagatgaagc gatgtcagtc ctgactcaat ccatcatcaa aaatcaggaa
      481 caggagatcc cgatccacaa atggaaggcc cctaaaatta aggatcttga agaatatcgg
      541 cccgggaccc tcaaggtaga ggaattcatg gagactgacc tcttcactgt acaaaaagat
      601 gatattgtcg agttggtggc tgaaatgatg gattggagga agatcagata tatgcctgta
      661 gaggataaaa agggcaacct gattggtctg gtgacgagcc gcttactatt acgccatttc
      721 tctaatcgta aggttgataa aaagaaggat gttatcattg tcaatgacat catgatcaag
      781 gacccaatta ctatttcgcc ggatgctact gtattggatg ctttggaagt aatgcgcaac
      841 aaacagattg gatgcctgcc tgttgttgac aaaaatgaat tggttggtat cattacagaa
      901 accgactttc tgcgtatcac aggaaggctg atcgaccgga tggaacatgt ggaagaagat
      961 ggagaatagg gtggtccaat aattgtttta tacaaataac tttgccataa aagccgattc
     1021 gattgtggat cggctttttt agtttagatg taatggatac tacggttagg gcacttccat
     1081 aaataattag gtaggaattt aatatccgat agagcatatt ttatcggtgt ttgatagcat
     1141 tatccttttt atttcttgta atctggacgg atattttagg tttgattctc tgaaagtgag
     1201 gatggtcatg gcgcccgggt attactcatc tacctacttc gcttcgggca gtttatcaca
     1261 ttttacgttt tgtctcaata cagtttataa attaaatgtt ttgaataata tttaccagag
     1321 gaatgggtga tatctaattg ataacctgtg ttttttttaa ttcttgtact tttatggaaa
     1381 aatcaattca gtcatgacct ttgaaataga aagcaatctc gtggacatcc cccagaaaag
     1441 tatttacccg gtacgcctga gggttgaaaa tggaagcatt gcccatataa tgcgtattga
     1501 ccggcaggtt accggctata ttctgccggg ctttgtagat gctcatgtgc atattgaaag
     1561 ttcactcctg gtgccttcag aatttgccag aatggcggtc atccatggca ctgtagcaac
     1621 tgtcagtgat ccacatgaaa tcgccaatgt ttgtggtgtt ccgggaattc aatatatgct
     1681 cgatgatgcc gacggcgtac ctttctattt caattttggc gcaccaagct gcgtgccggc
     1741 gactgtgttt gaaaccgcgg gcgacattat tggcccggag gaaataaagg catt
//
LOCUS       DAOQMC010000357         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_47664_length_1792_cov_2.559010, whole genome shotgun sequence.
ACCESSION   DAOQMC010000357 DAOQMC010000000
VERSION     DAOQMC010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47664_length_1792_cov_2.559010"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKV31_10280"
     CDS             complement(<1..535)
                     /locus_tag="PKV31_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNB93290.1"
                     /translation="MEFSPERVADLLRPEQRSARLYDLTGRLLISTGVILGCMLGLRF
                     FRQSLDSQIAGQAHYLSIDNTPAFQLPAATALPTLTLTPLPTATPTNTPSPTATPLPL
                     PTTRLSIPAIDLNTSVVESYPIQQRDGSFIWDPPNYAAGHYNSSGNPGGGRNIVFNGH
                     NNMAGEVFRDLNKLIPGD"
     gene            1288..>1792
                     /locus_tag="PKV31_10285"
     CDS             1288..>1792
                     /locus_tag="PKV31_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93291.1"
                     /translation="MTDENNEDTPDVQANNQSIAVGSISAGGNIGDIHIGNTNIYNTP
                     EDDIPLSSDEIENGLTRLIQYLPERAPILQDQFSSISKKLRTTLGADLNSLSPVLKSQ
                     REDGVNRMKLMCMEVTDISFRAMCLGQNPPPYDSRPPFLGLFAFHPEDREMADANHKA
                     ADFVQGTW"
BASE COUNT          529 a          387 c          424 g          452 t
ORIGIN      
        1 catctccggg aattagcttg ttgagatccc ggaacacctc ccctgccata ttattatgcc
       61 cattaaagac aatattccgg ccaccgcctg ggttgccact gctgttgtaa tgaccagctg
      121 cgtagttggg tggatcccaa atgaacgagc catcgcgctg ctgtattgga taactctcga
      181 caaccgaagt attcaaatca atggctggga ttgataatcg agttgtcggc aaaggcagag
      241 gcgtggccgt cgggctgggg gtatttgtag gtgttgctgt aggcaagggg gtgagtgtca
      301 gggtaggaag agccgtggca gccggtaatt ggaaagctgg tgtattgtca atgctcagat
      361 agtgtgcctg tccggcgatt tggctgtcca ggctctgcct aaaaaagcgc aagcccaaca
      421 tgcagccaag gatgactccg gttgaaatca ggagccgacc tgttaaatca tataaacggg
      481 ccgagcgttg ttcaggccgg agcaaatctg ccacccgttc cggagaaaat tccaacgaat
      541 aggattatgg taagcatggg aatgaccaaa cttccttcat tgtgcttagc attcaattgc
      601 ccaaaaatag aatacttctg gcagacaggg gtcattgcga attttctttg aattgaggtt
      661 tttgatacgg tagaggcagt gaaggcatca atgaacacct ccctctgata tattcttaag
      721 tcttcataaa atctgggtat cgttaaaaaa acaggccaat ttaacacatt gctttctgga
      781 ttctcattgc aaaagccaat attatttttt cattataatg aattcaggaa gaaaatcaag
      841 aattgccctg ccaggacttt gttatggtca aaactgggaa tagagatttg cttaaattag
      901 cagataaaaa ggccgttctt catgatttta aaaccttatt gatgtatctg aatttatgtc
      961 caagtatcgg atagaagtga ctagttgaca atgacggtct gaaaactata taataacaaa
     1021 agcatgggga actcaggaac agactggaaa cagtagtgaa cccaagggaa ttgttggata
     1081 tccgttaaag aactaatcgt tttaatttca ttcacctggg acgacgaagc cgtaaaaaac
     1141 taccaggcgc tggaattata gagtacctaa tggccagtga tggatgagaa agcatcagtg
     1201 taacagcatc aggttggccg taacgtgaac aaaaatattg cgaccagaaa ccataagaaa
     1261 taaatcctac gactaggatg gtacggtatg actgatgaga ataatgaaga cacccctgac
     1321 gttcaggcga ataaccaaag tattgctgtt ggaagtatct ctgcaggcgg aaatattggc
     1381 gacatccaca tcggaaacac caacatctac aatacccctg aagatgatat tcccctttcc
     1441 agtgatgaaa tcgaaaatgg actaacccgc cttatacaat accttcctga gcgtgctccc
     1501 atactccaag atcaattttc gtcgatttca aagaaactgc gtacaacact tggcgcagat
     1561 ctgaactcgc tcagccctgt attgaaatcc cagcgcgaag acggcgtcaa ccgcatgaag
     1621 ttaatgtgca tggaggtaac cgatatttcc ttccgtgcca tgtgccttgg acaaaatccg
     1681 ccgccttatg attcgcgtcc gccatttctc ggtctctttg ccttccatcc cgaagacagg
     1741 gaaatggcgg acgcgaatca taaggcggcg gattttgtcc aaggcacatg gc
//
LOCUS       DAOQMC010000358         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_47927_length_1787_cov_2.841801, whole genome shotgun sequence.
ACCESSION   DAOQMC010000358 DAOQMC010000000
VERSION     DAOQMC010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47927_length_1787_cov_2.841801"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..786)
                     /locus_tag="PKV31_10290"
     CDS             complement(187..786)
                     /locus_tag="PKV31_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNB93292.1"
                     /translation="MDEIIFEKIRSLKKHLEILHDELDKIPQMLDDGPYLASYDILTF
                     ENQGTLNPAWRDKCDEWMAAGRDFSEMVNSLNTIQTNKLLIQLKVVLDINLEYEMVKT
                     RGEWNNSWEHKQVNEKILLEKLLYVLAERKVHEAAPLLNQFLDHDIWPIQVIKTLGTV
                     GNEGSIERLRKKIKSNWEQEREAAWQALLGLEQNTQSHE"
     gene            complement(851..>1787)
                     /locus_tag="PKV31_10295"
     CDS             complement(851..>1787)
                     /locus_tag="PKV31_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93293.1"
                     /translation="IQCFPSMIFWCPMKKEKTGDDKKNMDGIRKELIIAGIGLFGTIL
                     AAVISAWGNWAQSKMIINATQTVVAIFAHPANTSTSQPITATPQETGQRWDQHFEVEM
                     IQSICQFTTQIIPEPFTSDLGKPDVLDIFYTAGTESWSFGPSTSYPVGYAINISNISA
                     NTVWPKISNSFQFTVSWVDNAPEHADVLVNSVGCGGGTWRDGDMIVSLSPEYISTRQK
                     VIFEDSDYYSLQRGESEVFIIQVQCKGPGIYLLDVDIPYTFSTDLDVINLKASKYIIC
                     PETYSRWDVNPSAHSIVFYKNLSWDSESQSYIEVK"
BASE COUNT          468 a          391 c          343 g          585 t
ORIGIN      
        1 gcctgcctgg tcaattaatt aacacgctcc tggtatcagc tactttttgc gtccatccat
       61 tcgatatggg aactgagttg ggagaaatga ggcttcggtt ggttgatgcc ctcgtcagtt
      121 ttttgattta tttcatgcgt cattacttga attgtaggtt ggtaatttta ttgccccgta
      181 atatttttac tcatgcgatt gggtattttg ttccaatccg agtaatgcct gccaggctgc
      241 ctctcgttcc tgttcccaat tgcttttaat cttctttcga agtctttcaa tagaaccctc
      301 gtttcccact gttcccaggg ttttaataac ctggattggc caaatgtcat gatccaggaa
      361 ttgatttaat agcggtgctg cttcatgcac cttcctttcc gctaatacat ataacaactt
      421 ttccagtaat atcttttcgt ttacttgctt atgctcccaa ctattattcc attcccctct
      481 tgttttgacc atttcatact ctaagtttat gtccaggacc accttcaatt gaatgagcaa
      541 tttgttggtt tgtatggtgt tcaagctgtt taccatttcg gaaaaatccc tgcctgcagc
      601 catccattca tcacatttat cccgccatgc agggttcaga gtcccttgat tttcaaaggt
      661 gagaatgtca taggatgcaa gatatggtcc gtcgtccagc atttgcggta tcttgtccaa
      721 ttcatcgtga agtatttcca gatgtttctt gagcgacctg attttttcga aaataatttc
      781 atccattagg tactccggag ttacagaatt caattaatat atgcggcatt cacaacatta
      841 aattatcttt ttactttact tctatatagc tttgactttc actatcccag gagaggttct
      901 tgtaaaatac gatactatga gcgctcggat tgacatccca tcgcgaatag gtttcaggac
      961 aaataatata tttggacgct ttcaaattga taacatccaa atctgttgaa aatgtatagg
     1021 gaatatccac atccaacagg taaatcccgg ggcctttgca ttggacttga attataaaaa
     1081 cctcactttc accacgttga agtgagtagt aatcagaatc ttcaaaaatc accttttgcc
     1141 ttgttgagat gtattccggg gaaagtgaaa ctatcatatc tccatcacgc caggtacctc
     1201 ccccacaacc tacactgttc acgagcacat cagcatgttc tggggcgttg tccacccagg
     1261 atactgtgaa ctgaaaagaa ttagagatct ttggccatac agtattggcg gaaatgttgc
     1321 taatattaat ggcgtagcca actggatatg aggttgaagg accaaatgac caggattcgg
     1381 tccctgctgt ataaaaaata tccaaaacgt caggcttccc aagatcagaa gtaaatggtt
     1441 ctggaattat ttgagtagta aattggcaaa tggattgaat catttccact tcaaaatgct
     1501 gatcccaacg ctgtccagtc tcttgaggtg ttgcagttat aggctgagaa gtacttgtgt
     1561 tggcagggtg tgcgaatatg gccacaaccg tttgagtggc attaattatc attttgctct
     1621 gggcccaatt tccccatgcc gagatcactg ccgcaagaat ggttccgaac aacccaatgc
     1681 cagctatgat cagttccttc ctgataccat ccatattctt tttgtcatca cctgtttttt
     1741 cttttttcat ggggcaccaa aatatcattg aaggaaaaca ttgaatg
//
LOCUS       DAOQMC010000359         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_48933_length_1768_cov_3.406888, whole genome shotgun sequence.
ACCESSION   DAOQMC010000359 DAOQMC010000000
VERSION     DAOQMC010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48933_length_1768_cov_3.406888"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1040)
                     /locus_tag="PKV31_10300"
     CDS             complement(393..1040)
                     /locus_tag="PKV31_10300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB93294.1"
                     /translation="MRTFIPYRYFCIEGNIGSGKTTLTKMLAEEFNALTLLEQFDENP
                     FLPPFYEDKERYAFPVEVFFLSERHKQMQMELSVSNLFHETVIADYIFHKTILFAREN
                     LDDREYALFYKLYQQLDNQLIHPELIVYLHRPVDALLKNIRKRNRSFEQNISGDYLAS
                     ISNSYLTYLSSQVDSPVIIFHLRDGDFENDRSLYAIIKDRIVQKGTATGVDQVYL"
     gene            complement(1037..1522)
                     /gene="folK"
                     /locus_tag="PKV31_10305"
     CDS             complement(1037..1522)
                     /gene="folK"
                     /locus_tag="PKV31_10305"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNB93295.1"
                     /translation="MARIVLSLGSNIEPREKHLKKAIAEIADWSESALKVSNMYSTEP
                     VGFRSEKDFLNLAVLFETSCSPAELLAKIHAVESKMGRIRTPGRVEDRIIDMDILFYD
                     DLILNTDELSIPHPRLHERSFVLTPLLDIVPDLVHPILHKTIWELYDECKDLSEVRIV
                     E"
     gene            complement(1588..>1768)
                     /locus_tag="PKV31_10310"
     CDS             complement(1588..>1768)
                     /locus_tag="PKV31_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93296.1"
                     /translation="ADTMTMKGICCVRYNNGERKDRSTVIQVSGYTLQDEKNEVEAGD
                     TLYYRLFDVRLIKKL"
BASE COUNT          547 a          402 c          301 g          518 t
ORIGIN      
        1 gacgggattt aattcttttt ttgtgatgaa tgaaaaaata gaagcagaag gaattgtcca
       61 acaggtatat ctttcacagc ctttcaaata ttgtgtagcg ggatgggtcg atacggatct
      121 tgccaacgag acagccattg cagatctgaa gctatacaaa tcaatataac cactcctatc
      181 aaagtcgctc cctgtgttcc actaatctgc tacttttaag gttgcctcaa aattcatcct
      241 cctcaaaaaa atattgttac cggaactgat caattctttc ctgtaaaatt gggaaaattc
      301 aatttactct tttgttatgc ttcatctcat atcaatattc accccctaaa gaaattcatc
      361 acccaatgga caaacccatg gccttcccgg gtttataaat agacctgatc aacccctgtt
      421 gcagttcctt tttggactat cctatccttg ataatggcat atagagaacg atcattctca
      481 aaatcaccat cgcgcaaatg gaaaataata acaggtgaat caacttgtga actcaaataa
      541 gtcaggtagg aattagaaat cgaggcgaga tagtctccgg agatattttg ctcaaaactt
      601 ctgttgcgct ttctgatatt ctttaacagg gcatccaccg gcctgtggag atatacgatc
      661 aattcaggat gaatcaattg attatcaagt tgctggtata atttataaaa aagggcatat
      721 tcccggtcat ccaaattttc cctggcaaat agaatggttt tatgaaaaat gtaatccgca
      781 atcactgtct catgaaacag gttgctaaca ctcaactcca tctgcatctg tttgtgacgt
      841 tccgaaagga aaaaaacttc caccggaaag gcatatcttt ctttatcttc ataaaagggg
      901 ggtagaaaag gattttcgtc aaattgttcc agcagagtga gggcattgaa ctcttcggca
      961 agcattttgg tcagcgtggt cttaccactg ccaatattgc cttctatgca aaaatatctg
     1021 taaggaataa atgttctcat tctacaatac gcacttcact caaatccttg cactcatcgt
     1081 acagttccca gatcgtttta tgcaatatcg gatgaaccaa atcgggtaca atatccaaca
     1141 agggcgtcaa tacaaaacta cgctcatgta atcgcggatg aggaatcgat aattcatctg
     1201 tattcagaat cagatcgtca taaaacaaaa tgtccatatc gatgatcctg tcttctactc
     1261 tacccggtgt tcggatcctt cccatcttac tttccactgc atgaatttta gccagcaact
     1321 cggcaggaga acaggacgtc tcaaacaaaa cagccaaatt aagaaaatct ttctcagaac
     1381 gaaagcctac cggttcagtg gaatacatgt tagaaacctt caaagccgac tctgaccaat
     1441 cggcaatttc ggctatagct ttcttcaaat gcttttcccg gggttcgata ttacttccga
     1501 gcgaaagaac tatcctggcc atttcaacga cttaaactta tgataaatga taaaaaaatt
     1561 ccgaccatgg cacttcatca aaattcctta caacttcttt attaaccgaa catcaaataa
     1621 tctgtaataa agtgtgtccc ctgcctccac ttcatttttc tcatcctgca gggtataccc
     1681 acttacctga attacggtag aacggtcctt tctctcacca ttattgtatc tgacacaaca
     1741 gattcccttc atagtcatgg tatcggcc
//
LOCUS       DAOQMC010000360         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_49036_length_1766_cov_4.306838, whole genome shotgun sequence.
ACCESSION   DAOQMC010000360 DAOQMC010000000
VERSION     DAOQMC010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49036_length_1766_cov_4.306838"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..>1766
                     /locus_tag="PKV31_10315"
     CDS             406..>1766
                     /locus_tag="PKV31_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNB93297.1"
                     /translation="MSRNLSELMGKSGLEKNLFEQIGRKAKDTGTPDVGEMERLAEEF
                     LIGKANVFGTASFYDFTRQTNEGKKVYICNGSACLTAGTQPAVMEKLKGHFKEEEIGE
                     MCCLGRCHENSAFHYKGHNYSGDAINRLNEIKNLPEVMMDEYRTGHIGTPILTGDPLT
                     LKNFREILGSCLHTGHESLLNELTVSGLRGRGGAGFPISIKLDTGRKAEGKSKFIVCN
                     ADEGDPGAYSDRYLLEKHPLKVLLGMMIAGFIIGADWGVLYIRGEYPESVDTIETAID
                     QLSRSGLIGENILGSGFKFKFKVVRGMGAYICGEETALLNSIEGQRPEVRTRPPFPAV
                     HGLFNRPTIVNNVETLACIPWIMRYGGKSYAALGIGRSTGTKLVSLDGHFNRPGIYEV
                     EMGTPLKDVVQELGGGFNTEIKALHIGGPLGGLIPVHRINDLSVDFESFSANGFLLGH
                     ASIV"
BASE COUNT          524 a          314 c          445 g          483 t
ORIGIN      
        1 atggttatgt cacatatcta caggattatc tcaatgaata ctgttaccga tataataggc
       61 atcgcatgaa aggagagatt ttggacgata ttattacgag aatggtcagg cataagccaa
      121 tggtacgaag gatttgttca gtctgatcag aagacgaagg gtgctttatc taataattat
      181 tttgagactg ttaaagataa agtaaaattg ttgtaaatct gcaacaaagt gtacaatgct
      241 tctttacaat gataatcaaa aagttagagt ggttagagaa aatgaggttt ttaattttgt
      301 tagattgtac taccctcgaa gctggaaatt acgaatatcc tactttccat ttgctcaagt
      361 tgctgaaaat tgctattttg cgtaaaaata ttgtaactta ataaaatgtc aagaaatctc
      421 agcgaattaa tgggcaaaag cggtttggaa aaaaacctct ttgaacaaat tggtaggaag
      481 gcaaaagata cagggactcc tgatgtaggt gagatggagc ggttggcaga agaatttctg
      541 atagggaagg ccaatgtctt cggaacagcc tcgttctatg attttacccg tcaaaccaat
      601 gagggtaaga aagtatatat ttgcaacggc tctgcctgcc taactgctgg cacacaacct
      661 gcagtcatgg aaaagttaaa aggacatttt aaggaagaag agattggtga gatgtgctgt
      721 ctgggtcgat gtcatgagaa cagcgccttt cattataagg gacacaatta ctccggtgat
      781 gccataaaca gactgaatga gattaagaat ttgcctgagg ttatgatgga tgaataccgg
      841 acagggcata tcggcacacc aatacttact ggtgatccct tgactttaaa aaactttagg
      901 gagattcttg gaagttgctt gcatacaggg catgagtcac tgttaaatga gttgacagtg
      961 tctggattga ggggcagggg tggagcaggt ttcccgattt caatcaaatt ggataccggc
     1021 aggaaagcag agggcaaaag caaattcatt gtttgcaacg ccgacgaggg tgatcccgga
     1081 gcatacagtg atcgctactt gttggaaaaa cacccactta aggttttact tggcatgatg
     1141 attgcaggat ttatcatcgg agctgattgg ggtgtactat atattcgtgg tgaatacccg
     1201 gagagcgtgg ataccataga gactgccata gaccaacttt cgcggtctgg tctgattgga
     1261 gaaaacattc tgggcagtgg attcaaattc aaattcaaag tagttagggg gatgggtgca
     1321 tacatctgcg gtgaagagac cgctttgctc aattctattg agggtcagcg cccggaagtt
     1381 cgtacaaggc caccattccc tgcagttcat ggattgttca accgaccgac tatcgtcaac
     1441 aatgtcgaga cactggcctg catcccctgg attatgagat atggaggtaa atcatatgca
     1501 gccctgggta ttggtcgatc caccggaacc aagctcgttt cgttggatgg ccacttcaac
     1561 cgtcccggta tctatgaggt tgagatgggg acacccctta aggatgttgt acaggagctc
     1621 ggaggcggct ttaatactga aattaaagca ttgcatatcg gagggccctt gggaggatta
     1681 attcctgtgc atagaattaa cgacctgagc gtggattttg agtcattttc ggcaaatgga
     1741 ttccttcttg gacatgcatc catagt
//
LOCUS       DAOQMC010000361         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_49426_length_1758_cov_4.436876, whole genome shotgun sequence.
ACCESSION   DAOQMC010000361 DAOQMC010000000
VERSION     DAOQMC010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49426_length_1758_cov_4.436876"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKV31_10320"
     CDS             <1..553
                     /locus_tag="PKV31_10320"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNB93298.1"
                     /translation="LNKLASLAGQAFDQDGLTARKGILQPELIIALNKMEYFAAPYPK
                     SLDNNWIMKEFYPVIKNFDYSPEDKLNTLCNFIAQRISQELKLICQREQTNFSGKNML
                     VTGGGAYNQYLMECIAEICRNENGIQVSIPPKTIIEFKEALLMALAGLLRIKNIPNMM
                     KTVTGSMVDNIGGCIYQGTNVLI"
     gene            596..1636
                     /locus_tag="PKV31_10325"
     CDS             596..1636
                     /locus_tag="PKV31_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB93299.1"
                     /translation="MEKEAYRILVTGATGLVGSYVVRTLIANGYSDITIIRRATSNNA
                     LIHDVVDRLKIVETDLNDLVGLEAAMRDRTHVIHCGALVSFDSRDRRPLIKTNVEGTA
                     NLINLSVEFGIKKFIHMSSIAALGRNSQEGGVTDEYTPFEENPSNSYYSITKYQGELE
                     VWRGKAENLDVRVLLPGLILGSGFWKVGTNGFFNHVWRGIPFYPTGTTGFVDVRDLAE
                     FAMRMLNYQGVEEKFLMIGHNVTYRQFLDTIAKALGKKTPYIRARKWILGVAWQLDKL
                     RSLVTGSRPMLTRETATTSSKQYLFDNSKSLSIPGFQYTDFDQTIKDIAGQYLEASNN
                     NFQPSVLKVHRI"
BASE COUNT          553 a          352 c          389 g          464 t
ORIGIN      
        1 attgaataaa ctggcttcac tggcaggtca agcattcgat caggatggcc ttacagcacg
       61 gaaaggcata cttcaacccg aattgatcat cgcactaaac aagatggagt attttgcggc
      121 gccttatcca aaatctctgg ataacaactg gataatgaag gaattctacc ccgtcatcaa
      181 gaactttgat tacagcccgg aagataaatt gaatacattg tgtaatttca tcgcccaacg
      241 aatttctcag gagctgaaat taatatgcca acgtgagcaa acaaactttt ctggaaaaaa
      301 catgttggtc acaggaggtg gagcttacaa tcaatacctc atggagtgca tagccgagat
      361 atgtcgtaat gaaaacggga tacaggtttc aattccaccc aagaccatca ttgaatttaa
      421 ggaggctctc ttaatggctt tggcaggact gctcagaata aagaatatac ctaatatgat
      481 gaaaacagtt acaggatcaa tggtcgataa tatcggtggc tgcatatatc aaggtacgaa
      541 tgtcctgata tgaagacccg ttctttattc tcaagattga aaaaaatgtt ttaatttgga
      601 aaaggaagca tacaggattc ttgtaaccgg agcaaccggc ttggtgggtt cctatgtagt
      661 aagaaccctg atagcgaatg ggtattctga tattacaatc atcagaagag caacttctaa
      721 caatgcattg atccatgatg ttgttgatcg gttgaaaatc gtggaaacag acctgaacga
      781 cctcgttgga ttagaggcag ccatgcggga tagaacacac gttatacatt gcggtgctct
      841 ggtttcattc gattcccgtg accgcaggcc actaataaaa accaatgtgg agggaactgc
      901 caacctcatt aacttaagtg tggaattcgg gataaaaaaa ttcatccaca tgagttcaat
      961 tgctgctttg ggccgaaaca gtcaagaagg gggtgtcaca gatgaataca caccttttga
     1021 agaaaatcct tcaaattcct attattcaat taccaaatat cagggtgaat tggaagtatg
     1081 gcgcggcaag gccgaaaatc ttgatgtcag ggttttactg cccgggttaa tccttggtag
     1141 cggtttctgg aaggtaggca ccaatggatt tttcaatcat gtttggcggg gaattccctt
     1201 ctaccctacc ggaacgacag ggtttgtcga tgttcgggat ctggctgaat ttgccatgag
     1261 gatgctcaac taccagggag ttgaagaaaa attcctgatg ataggacata atgtgactta
     1321 cagacaattt ttggatacca ttgcgaaagc attgggcaag aagactccat atatcagagc
     1381 ccgcaaatgg atactcggcg ttgcctggca attggataaa ttgcgttccc ttgtcacagg
     1441 aagccgaccc atgctaactc gtgaaactgc caccacttcg tctaagcagt atttgtttga
     1501 caacagcaaa tcgctgtcca tacccgggtt tcaatatacc gactttgacc aaactattaa
     1561 agatatagcc ggacaatacc tggaagcttc taacaacaac tttcaaccgt ccgttttaaa
     1621 ggttcatagg atatgaatga caatatagat aaaattgtaa gtttctccag gcaaatcctt
     1681 caaaaggaaa aatccatcca ttcaaaggaa atgatgaatg agttggtgga tgccattcga
     1741 ttccacgagt atcggtat
//
LOCUS       DAOQMC010000362         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_50302_length_1742_cov_4.977475, whole genome shotgun sequence.
ACCESSION   DAOQMC010000362 DAOQMC010000000
VERSION     DAOQMC010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50302_length_1742_cov_4.977475"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKV31_10330"
     CDS             <1..305
                     /locus_tag="PKV31_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93300.1"
                     /translation="WDSLIVWTGGELKASHNLAYNDSLPFNIVFGYPPNVNGRYSGGG
                     IQASFWSADEYNSSTAWVYYFLKEKLPLTFSNYFSKNYGMMCRCVKTIADSSSETN"
     gene            432..>1742
                     /locus_tag="PKV31_10335"
     CDS             432..>1742
                     /locus_tag="PKV31_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93301.1"
                     /translation="MKKMTGFLLGFLVWSLGVFAQTSGGLFSYGPDMNSTRIFPYVSQ
                     KPDGDVMVFGGRSNGFISSAYADLYSIETNTFTNLNMLYTHDNTVMVKLNDGRVLLAG
                     GGFDYGVPAHQYAEIYNPNDNSFTAAGTMAYARMQGSGALLTDGKVLIVGGWYSPEAA
                     ANAELYDPVLMTSSYAGGLLNPRSNALVLPCNDGGALVIGGYPTYGGTTFNAVEYYNR
                     ETSSFELFSDEIISGETGWTTTLDPFYRKTTDMYILENGKYLLMLYRSVPTLEYTLAL
                     FDPENKSFEKVNTDYPLMGAYSDGGIIDYALNKEESLVYMLGVDNESGSTGYCIVTTD
                     LSTGKTYQPDESYTMPSGEYLIFTTLSYIPETGMLLAPGVSTSNTDYFNATAKSFLIE
                     PELKVDIPIQEDLIVLQVYPNPVKDMLMLILPSNIGGQAIVDICD"
BASE COUNT          503 a          306 c          377 g          556 t
ORIGIN      
        1 aatgggattc tcttatcgta tggacaggtg gagagttaaa agccagtcat aatttagcat
       61 ataacgacag tcttcccttc aatatcgttt ttggctatcc gccgaatgta aacggacgat
      121 attccggtgg cggcatacag gcgagtttct ggtcggccga cgaatataac tcctccactg
      181 catgggtgta ttattttttg aaggaaaaat tgccgcttac cttcagcaat tacttctcta
      241 aaaactatgg catgatgtgt cgatgcgtta aaaccatagc ggattcttct tcagaaacta
      301 attaattatt gtttttatct gttgacaaat atcagatatc acaatagtga tatgtgttta
      361 ctatgtgatt gacagaggtc atactactta cgcaaagttt aggtgacttt tgattagcta
      421 aactatgagt tatgaaaaag atgacaggat ttctcctcgg atttctggta tggagtttag
      481 gagtatttgc tcaaacaagt ggtggtttgt tttcctacgg acctgacatg aattctacac
      541 gcattttccc ttacgtttca caaaagcctg atggagatgt catggttttt ggcggcaggt
      601 caaacggatt tataagttct gcatatgccg atttatatag catagaaacc aatacattca
      661 ctaatcttaa tatgttatat acgcatgata atacggtcat ggtgaaattg aatgatggtc
      721 gtgtattgct ggcaggtggc ggttttgatt atggggtgcc tgcgcatcag tatgcagaaa
      781 tttacaatcc taatgataat tcttttactg cagctggaac tatggcttat gcacgaatgc
      841 aaggttcagg agcattgctt actgatggca aagtgttgat cgtaggtggt tggtatagtc
      901 cggaggctgc tgccaacgca gagttgtatg accctgtatt aatgacctca tcatatgccg
      961 gtgggttact taaccctcga tccaatgcat tggttttgcc ttgcaatgat ggtggagcgc
     1021 ttgtaattgg agggtatcca acttatggcg gcacaacatt taatgccgta gaatattata
     1081 atcgggagac aagttcattc gaattatttt ctgatgaaat tatttccgga gaaaccggct
     1141 ggactactac gcttgatcct ttctacagga aaacaacaga tatgtatatc ttggaaaatg
     1201 gaaagtattt gttgatgctc tatagaagtg ttccaacatt ggaatacact ttggcactgt
     1261 ttgaccctga aaataaatct tttgagaaag tgaatacaga ttacccacta atgggtgcgt
     1321 acagcgatgg tggaattatt gattatgcgt tgaataaaga agagtcattg gtatatatgc
     1381 tgggtgtgga taatgaatca ggcagtaccg gatattgcat agtgacaaca gatttatcaa
     1441 caggaaaaac ttatcaacct gatgaatcct ataccatgcc ttccggcgaa tatttaatat
     1501 tcaccacact ttcctatatc cctgaaaccg gaatgttact tgctccgggt gtttcaactt
     1561 caaataccga ttattttaat gctacagcta agagttttct tattgagcct gaattaaaag
     1621 ttgatattcc tatacaagag gatcttattg ttttacaagt atatccaaat ccggttaaag
     1681 acatgctgat gctcatactt ccttcaaata tcggaggtca ggcaatagtg gatatctgtg
     1741 at
//
LOCUS       DAOQMC010000363         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_50498_length_1739_cov_4.318290, whole genome shotgun sequence.
ACCESSION   DAOQMC010000363 DAOQMC010000000
VERSION     DAOQMC010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50498_length_1739_cov_4.318290"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1739
                     /locus_tag="PKV31_10340"
     CDS             <1..>1739
                     /locus_tag="PKV31_10340"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNB93302.1"
                     /translation="PDTTPWQISYDGRKNEPVHLPVKFPLLLSQGTEGIAVGLATKIL
                     PHNFNELLDACVAYLRNEPFVLYPDFLTGGFADVSNYNSGNRGGRVLSRAKIEIPNRN
                     MLEITELPFGVTTDRLIESILKANDKGQIKIKKITDNTARDVNISIELMPGISPDQTM
                     DALYRFSDCEVSISTMCCIIHDDKPVFIGVEEVLKANVDQTKQLLKQELQIKLDELTE
                     KWFFTSLEKIFIEQRIYRDIEEAESFEQVISIIRAGLEKYVSTPTHPEPTIKLLAKLL
                     RDINEDDIIRLTEIKIKKISKYSSFETDQALKTLLEEISKVKHDLKHLVEFTIQWFTY
                     LKDKYGKGKERKTILTQFEAIEATAVVQNNSKLYVNREEGFVGFGLKKDEFVSDCSDI
                     DDIIVFTAQGKMMVTRLSEKTFVGTNIIHVAVWKKNDERTTYNMIYSDGPGGKAYAKR
                     FNVTAITRDKEYDLTKGAAKPRLHYFSANENGEAEIVKVTLSQTSKAKIKVFDFDFST
                     QLIKGRDSQGNIVTKYPVRKITLEEKGGSTLGAQVLYFDEETGKLNVDDKGKKIGSID
                     NGDKLVIFYSNGSL"
BASE COUNT          567 a          328 c          396 g          448 t
ORIGIN      
        1 cctgacacga ccccctggca gatttcatat gatggaagga agaacgaacc ggtacatttg
       61 cccgtcaaat ttccactctt gctcagccag ggtaccgaag gcatcgctgt cggcctggca
      121 acaaaaattc ttcctcataa ttttaatgaa ttattggatg cttgcgtggc ctatttgaga
      181 aatgaacctt tcgtattata cccggacttc ttaaccggtg gctttgccga tgttagtaat
      241 tataattcag ggaatcgggg aggccgtgtc ttgtcaaggg cgaaaataga gatacccaac
      301 aggaatatgc tcgaaatcac tgaactacca ttcggggtga ctaccgaccg attgatagag
      361 agtatattga aagcaaatga caaaggacaa atcaaaatca aaaaaatcac ggataatact
      421 gccagggatg tcaatatttc tattgagctg atgccaggga tttctcccga tcaaacgatg
      481 gatgccttgt atcgattttc tgactgtgag gtaagcattt ccaccatgtg ctgtatcatc
      541 catgatgaca agccggtctt tataggagtt gaagaggttc ttaaagctaa tgttgatcaa
      601 acgaaacaac ttctaaaaca agaattgcag atcaaactgg atgagcttac tgaaaagtgg
      661 ttctttacca gccttgaaaa aatatttata gagcaaagga tctaccggga tattgaggaa
      721 gcggaaagct tcgaacaagt gatttctatc attcgtgcag gattggaaaa atatgtatct
      781 acgccgactc atcccgaacc gactatcaaa ttgctggcca aattactacg ggatatcaat
      841 gaagatgata tcatccgtct tacagaaatt aagataaaga aaatctccaa atacagcagt
      901 tttgaaactg atcaggcgtt gaaaactctc ctggaagaaa tctccaaggt gaaacacgat
      961 ctcaaacatc tggttgaatt caccattcaa tggtttacct atctgaagga caaatacggt
     1021 aagggtaaag aaagaaagac catcctgacc cagtttgaag ccattgaagc gacagcggtc
     1081 gtccagaata attccaagtt gtatgtcaac agggaagagg gctttgtagg tttcggcctc
     1141 aagaaggatg agtttgtatc tgattgctcg gatatcgatg acatcatcgt ctttactgcg
     1201 cagggcaaga tgatggttac aaggctgtcc gagaaaacct tcgtaggtac caatataata
     1261 catgtggcag tttggaagaa aaatgatgag cggactacct ataatatgat ctattcggat
     1321 ggtccgggag gaaaggccta tgccaagaga ttcaatgtca cggctattac acgggataaa
     1381 gagtatgatc ttaccaaggg tgctgcaaag cccagactgc attatttttc agcaaatgag
     1441 aatggtgaag cagaaattgt taaggtaact ctttctcaaa catctaaagc gaagataaag
     1501 gtgtttgatt tcgactttag tacgcaactg atcaaggggc gtgattctca aggtaatatc
     1561 gtcacaaaat atccggtcag aaaaatcaca ctggaggaaa aaggtggttc gacgctcggc
     1621 gcacaggtac tttattttga tgaagaaacc ggaaaactca atgtggatga taaaggtaaa
     1681 aagattggaa gtattgacaa cggagacaaa ctggtaatat tttattccaa tggtagcct
//
LOCUS       DAOQMC010000364         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_50547_length_1738_cov_3.973856, whole genome shotgun sequence.
ACCESSION   DAOQMC010000364 DAOQMC010000000
VERSION     DAOQMC010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50547_length_1738_cov_3.973856"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PKV31_10345"
     CDS             complement(<1..854)
                     /locus_tag="PKV31_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB93303.1"
                     /translation="MPRILIAGLGSIGLRHLNNLLQLGEKDILLLRTRNEPVKEAPDL
                     PVFTSLQDALNQKPDAVIVSNPTSHHMEVAVQAAKAGCHLFLEKPLSHTWENVNELLA
                     AAKEKGLIGMVGFDMHFDPGLQKVRQLIKDGTIGHITAIQAQVGQYLPDWHPWEDYRK
                     GVSAQVKTGGGVILDLIHEIDYVTWLMGKAKEVVSMNGHVSSLEIETEDTASILIRFQ
                     NNAIGTINLDYVQRTLSRSSRIVGEEGTLTWDLMNQKVSWYLAEDKAWHDFSYAGFQR
                     NDRFLAEM"
     gene            1177..>1738
                     /locus_tag="PKV31_10350"
     CDS             1177..>1738
                     /locus_tag="PKV31_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93304.1"
                     /translation="MNLILFTASYPYVRGNEASFLNIEVEYLSKVFDRVLVVPETIKE
                     STPVNHPPVEVNTDYAEMLASKKISEISRSAFTSHILPKGIMELGFPLFSPTAWRRII
                     AFSGKAELTRRWVDDFLKHHHYVPKESLFYTYWLDHAVAGIGMLREKYPELALISRAH
                     GYDIYEEQYYDPPFWPCRQTVLNSMDK"
BASE COUNT          472 a          372 c          364 g          530 t
ORIGIN      
        1 ttcatttcag caaggaagcg gtcattgcgt tggaaacctg catacgagaa atcatgccag
       61 gctttatctt ccgccagata ccatgagact ttttgattca tcagatccca ggtaagagtt
      121 ccttcctcgc caacgatgcg actggagcgc gaaagtgtac gctggacata atcgagattg
      181 atcgtgccga tggcattgtt ctggaagcgg attaggatcg atgctgtatc ttcagtctcg
      241 atctctaaag aactgacgtg tccattcatc gatacaacct cttttgcttt ccccattaac
      301 caagtcacat agtcgatttc gtggatcaaa tccagaatca cgcctccacc agttttcact
      361 tgggcgctga cacccttacg gtaatcttcc catggatgcc aatcgggcag gtattgccct
      421 acttgcgctt gaatggcagt gatatgtccg atcgtgccat cctttatcaa ttggcgtact
      481 ttctgaagac cggggtcaaa atgcatatca aagccgacca tgcctataag ccctttttct
      541 ttagcagccg ctaatagctc attcacattc tcccaagtat gagaaagcgg tttttccagg
      601 aacaaatggc agcccgcctt ggcagcttgc acggcaacct ccatatgatg tgaggtcggg
      661 ttgctgacga taacagcatc tggtttttgg tttaaggcat cttgcaggct ggtgaataca
      721 ggcagatcag gagcttcttt tacaggttcg ttccgggtgc gtagtaacag aatatctttt
      781 tctcccaatt gaagcagatt gttcagatga cgcagaccga tggaacccaa tccggcgatc
      841 aggatacgag gcatttgatt ctccgggata atagcgggga tttgaggagc tatttattaa
      901 cacatattgt actagacttg ctaattgcat cacaaatatt cccatccata taattgaagt
      961 acaatttatc ggaagttttt cataaagaaa ttcccgacat gatagtattt ttatttaatt
     1021 tggcataaat ctatttttac aactaataac ttggtttttg tttgcaagtc ttactgcttt
     1081 atagatgaat gtccttacac ctcgctttac atttctttta atgtactaag aaatttgcaa
     1141 tgcgtaacga ccttctttaa agaaagagag atacacttga atttaatctt atttacagct
     1201 tcttatcctt atgtgagagg gaatgaagcc agctttttaa atattgaagt tgaatattta
     1261 tcgaaggttt tcgaccgtgt tttagttgtg ccggaaacta taaaggaatc tacaccagtt
     1321 aaccatccac ctgttgaagt aaatactgat tatgctgaaa tgttagcatc caagaaaatc
     1381 tcggaaatat cacggtctgc atttacttcg catattttgc caaaaggcat catggagttg
     1441 ggattccctc ttttttcgcc tactgcctgg agacggataa ttgccttctc aggtaaggca
     1501 gaattaaccc ggcgatgggt tgatgatttt ctaaaacatc accattacgt accgaaagag
     1561 agtctgttct acacttattg gcttgaccac gccgtggcag gtattggaat gcttagagaa
     1621 aaatatcctg agcttgcatt gatctcgcgc gcacatggtt acgatattta tgaagaacag
     1681 tattacgacc ctccattttg gccctgtcgg caaactgtct tgaatagtat ggataaag
//
LOCUS       DAOQMC010000365         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_51994_length_1713_cov_4.392039, whole genome shotgun sequence.
ACCESSION   DAOQMC010000365 DAOQMC010000000
VERSION     DAOQMC010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51994_length_1713_cov_4.392039"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1713)
                     /locus_tag="PKV31_10355"
     CDS             complement(<1..>1713)
                     /locus_tag="PKV31_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93305.1"
                     /translation="DQAKYLEQVKQLASSFGKDYDVKTYSFGEKSREGLAEKFSDKST
                     DISEVLTTVTDIYNNQNLGAIILATDGINNQGSDPGYTAGLINVPVYPIALGDTIPKK
                     DLYIKKVYHHDIAYLKDKFTIQADIAARNCQGKSTHWQLSRIDGKTSQILQKGNLTID
                     NKDFFKSVDMVLDADKPGVQHYRISLSKVDGEMTTVNNVKDFYVDIIDSRLKILILAN
                     GPHPDISAIRQVISTNQNYVVETSLLADFKKKVTDYDFVILHQLPSRNSDVSNILQQL
                     DAAKKPRLFVVGTQTDIYKFNKIQSLLTINGDGRNTNDAQASLVQNFGLFTLDPSVSQ
                     AIEQFPPLTTMFGDYKLTGNASVLLMQKIGKIDTNYPLLMFGEFNGIKTGILAGEGIW
                     KWRLFDYLQRQNHNIIDQLIDKSIQYLSVKEDKRKFRVTMPKNIFLENEQISFDAEVY
                     NNSYELINDGDVSMTIKNENGKEFPYTFSKTGKYFNLNAGYFPPGAYSFVAKTSGIGD
                     KLIFEGKFEVQPVQLELYETTANHGLLKLLAQSTSGQMVYKDEMDKLHNLVVSSDRLK
                     PVLY"
BASE COUNT          467 a          362 c          336 g          548 t
ORIGIN      
        1 gataaagaac tggctttaac ctgtcggatg aaacaaccag attatgaagt ttgtccattt
       61 catccttata caccatctgt ccactagttg actgcgcaag caatttcaac agtccatgat
      121 tggctgtggt ttcgtagagt tccagctgta ccggttgtac ttcaaatttt ccttcgaata
      181 tcagcttgtc tcctatgccg gaagtcttcg ctacaaaact atatgccccc ggtggaaaat
      241 atcctgcatt caggttaaaa tattttcctg tcttgctgaa tgtgtatgga aactccttac
      301 cgttttcatt ttttatggtc atagatacat caccgtcatt gatgagctca taagaattat
      361 tatagacttc agcatcgaag ctgatctgtt cgttttcaag gaaaatattc ttaggcattg
      421 tcaccctgaa tttcctttta tcttccttca cagatagata ctgaatgctc ttatcaatga
      481 gttggtcaat gatattgtga ttctgacgct gcagataatc aaacagtcgc cacttccaga
      541 tgccttcacc agccagtatt ccggtcttga taccattaaa ttctccaaac atcaatagag
      601 gataattggt gtcgatttta ccaattttct gcataagcaa aacgcttgca ttgcctgtaa
      661 gcttataatc accaaacatg gtagtcaatg gtggaaattg ttcaatggcc tggctgacag
      721 acggatcaag cgtaaaaaga ccaaaattct gaacgagcga cgcctgtgca tcattggtat
      781 tgcgcccgtc accgttaatg gtcaacaatg actgaatttt attgaatttg taaatgtccg
      841 tctgcgtacc cacaacaaac aaacgtggct tcttggcggc atcgagctgt tgcagaatat
      901 tgctgacatc actgttcctt gacggcaact gatggagaat gacaaagtca taatcagtaa
      961 ctttcttttt aaaatccgca aggagtgaag tctctaccac ataattttgg ttggtagaaa
     1021 tcacctgacg tatggcagaa atatccggat gcgggccatt tgccagaatg agtatcttta
     1081 acctcgagtc gatgatgtca acgtaaaaat ccttcacatt attgacggtg gtcatttctc
     1141 catctacttt actaagactt atcctataat gctgaacacc gggtttatcg gcatcaagca
     1201 ccatatcgac cgatttgaag aaatccttgt tgtcgattgt cagattgcct ttttgaagta
     1261 tttgggatgt ttttccgtct atcctactta gttgccaatg tgtagatttt ccctgacagt
     1321 tcctggctgc aatatcggct tgtatggtaa atttgtcttt cagatatgca atatcatggt
     1381 ggtaaacctt cttaatataa aggtcttttt tggggatagt atcaccgagt gctatcggat
     1441 aaaccggaac attaatgagg cctgctgtat agccgggatc actaccctga ttgttgatac
     1501 cgtctgtggc gagaatgata gcacccagat tctggttgtt gtagatatcc gttacagtag
     1561 tcaggacttc agaaatatca gttgatttgt cagaaaattt ttcggcgaga ccttcccttg
     1621 atttttcacc aaaactgtag gtttttacat cataatcctt accaaacgaa gatgcaagtt
     1681 gctttacctg ctcgagatat tttgcctgat ccc
//
LOCUS       DAOQMC010000366         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_52097_length_1711_cov_3.929348, whole genome shotgun sequence.
ACCESSION   DAOQMC010000366 DAOQMC010000000
VERSION     DAOQMC010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52097_length_1711_cov_3.929348"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKV31_10360"
     CDS             <1..537
                     /locus_tag="PKV31_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB93306.1"
                     /translation="LCPDQKGLVRYQGCPDTDGDGVPDNLDQCKDKAGPAETNGCPDT
                     DGDGIPDIEDKCPTVAGIPELNGCPAANQADLNILTNVSRNIKWQDNAFALSNDARNQ
                     LDKIVSLLKKNKKYKLTVIGYSDSDTPKDQITSLSNARADEVKGYLVSKGIEEQRIIA
                     KGGNLPKISTRKILFEVK"
     gene            578..>1711
                     /gene="guaA"
                     /locus_tag="PKV31_10365"
     CDS             578..>1711
                     /gene="guaA"
                     /locus_tag="PKV31_10365"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNB93307.1"
                     /translation="MTHQKVIILDCGSQYTQLIARRVRELNIFCEIFPYNKVPQIETD
                     VKALIISGSPFSVRDNNAPEIDYKNILGKLPMLGVCYGAQYLAQKEGGRVERSEQREY
                     GKAILNIETPDDPIFSGIPASTQVWMSHGDSVLELPPGYTNIATTEHNKFAMFKKNPI
                     NDEAPVYAVQFHPEVTHSSDGLKFLGNFLLNIANIKADWTPDAFIEESIDQLKTKIGD
                     EKVLMALSGGVDSTVCATLLQKAIGDNLICFFIDNGLLRKNEFNNVLNAYKDLKLNVV
                     GIDASDYFYEQLSGKTDPEAKRKIIGKGFIDIFEREAGKLGDISWLGQGTIYPDVIES
                     ISVHGPSVTIKSHHNVGGLPEHLKLKLIEPLRMLFKDEVRRVGI"
BASE COUNT          548 a          317 c          381 g          465 t
ORIGIN      
        1 ctatgcccgg accaaaaagg acttgtccgt tatcagggtt gcccggacac ggatggcgat
       61 ggtgtacctg ataatttaga tcaatgtaaa gataaagcag ggccggcaga aactaacggt
      121 tgtccggaca ctgacggaga tggaatccct gacatagagg ataaatgtcc aactgtcgca
      181 ggaattccgg aattaaatgg ttgtccggct gccaaccagg ctgatttgaa tattttgaca
      241 aatgtatcca gaaatatcaa atggcaggat aatgcatttg ccctgtccaa tgatgctcgt
      301 aatcaactgg ataaaattgt ttccttactt aaaaagaaca agaaatacaa gctgactgtg
      361 attggctatt cggattcaga tacaccaaaa gatcaaatca cctccctgag caacgcaagg
      421 gcagatgagg tcaaagggta tcttgtaagt aaaggaattg aagaacaaag gataattgcc
      481 aaaggtggta acttacctaa aatatcaaca agaaaaattt tatttgaagt taaataatca
      541 ggggttatac ctttgctact caaatcctat ctgaactatg acacaccaaa aggttatcat
      601 acttgattgc ggctcccaat atactcaact aatagccagg agagtaaggg aattgaatat
      661 tttctgtgaa atatttccat acaacaaagt tcctcaaata gaaaccgatg tcaaagcatt
      721 aattatttca ggaagtcctt ttagtgttcg tgataataac gctccggaaa ttgattacaa
      781 aaacattttg ggtaagttac caatgctggg tgtttgttat ggtgcgcaat atttggctca
      841 aaaggaaggt ggccgggttg aacgtagtga gcagagagaa tatgggaaag ccatcttgaa
      901 tattgagacc cctgatgacc cgatattctc cggaattccg gcatcaaccc aggtatggat
      961 gtcccatgga gattcagtat tggaacttcc tccgggttat acaaacattg caaccacaga
     1021 acataacaaa tttgcaatgt tcaagaaaaa cccgattaat gacgaggctc ccgtatatgc
     1081 agtacagttc catcccgagg ttacccacag cagtgatggc ttaaaatttc tgggtaactt
     1141 tttattgaat atcgccaata taaaggctga ttggacgccg gatgccttca ttgaggaatc
     1201 aattgatcag ttgaagacaa aaatcggtga tgaaaaagta cttatggccc tctccggtgg
     1261 tgttgatagt acagtttgtg ctaccttact tcagaaggca ataggcgaca acctgatctg
     1321 cttctttatt gacaatggat tgttgcgtaa aaatgaattt aataatgtat taaatgccta
     1381 taaggatctg aagctgaatg ttgtaggcat cgatgcatcc gactattttt acgaacagct
     1441 ttccggaaag acagatcccg aagctaagcg caagattatt ggaaaaggct tcatagatat
     1501 ctttgaaagg gaagcaggta aacttggaga tataagttgg ctcggtcaag gtaccattta
     1561 tccggatgta atagaatcca tctcagtgca tgggccaagt gtcactatta aatcccatca
     1621 taatgtggga ggattacctg agcatttgaa attgaagctt attgagcccc ttcgtatgct
     1681 ttttaaggat gaggttcgcc gtgtgggcat t
//
LOCUS       DAOQMC010000367         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_54301_length_1675_cov_7.348765, whole genome shotgun sequence.
ACCESSION   DAOQMC010000367 DAOQMC010000000
VERSION     DAOQMC010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54301_length_1675_cov_7.348765"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1387
                     /locus_tag="PKV31_10370"
     CDS             134..1387
                     /locus_tag="PKV31_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93308.1"
                     /translation="MKENDQSRFIRWGVPGWTMLLAFASFTLLDILLSPSSSKNSLVD
                     VIKTIVAIISLNAAASAAAALLVAAAGIPLGFVIYQAYFYIRWNSPFSRDGFFPPFIV
                     GRLNDLERTMEGVHKHFIVGDDKWRREWTNNPLYNSDHGLKWRYIENLFTEIVQYMDS
                     KLSGLSLHVRYRYLMDLMHTLGAALFGIYLGYLGYILLKVKTDDISLSIILSISIVCL
                     FFLMILLEMEDKVKKGKVKPVPKDKIHYLVVTPIKRFDLFQLSNPSANYLLFLFTLLY
                     MGSPSPYTVTYTPSHFVFRIAVVVAVVLAWLIPKLAYPKRVIISEILTLAVLEILSVM
                     ASKYINDWSAFKPDWWNIGWCILFFLVTNMIFIKNRQNTRDDLIAMQNYTINRYFSNS
                     IQPFEPPQKTSGIKRKTPKLKRRKK"
     gene            1475..>1675
                     /locus_tag="PKV31_10375"
     CDS             1475..>1675
                     /locus_tag="PKV31_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93309.1"
                     /translation="MTYEQQFLDALKSIFIGAKVEGDSGYINLMKIKASYFEKGVFPV
                     LMKDIDTACKPFEKGFREEMFDK"
BASE COUNT          470 a          342 c          359 g          504 t
ORIGIN      
        1 tcacccgttc aagatgtgta gcgcccctgc cgccaaggac ggcggcttgt gcggcagtgg
       61 ggatgtcatg ctaaaaagat gtgaggtata ctttgaaggt cgatcaaaaa atcatatatt
      121 agggaggtga ttcatgaaag aaaacgatca atcaagattt attcgatggg gggtgcctgg
      181 ctggacaatg ctcctcgctt ttgcgagttt cactttactt gacatattac tatccccttc
      241 gtcaagtaag aatagtctgg tagatgtaat taagaccatt gttgccatta tttcactgaa
      301 tgccgctgct tctgcagcag ctgctttgtt ggttgctgct gcaggcatac cgcttggctt
      361 tgtcatttat caggcttatt tttacatccg atggaattcc ccattttcga gagatggttt
      421 ttttccgccc ttcattgttg gcagactcaa cgatctcgaa cgtacgatgg agggagttca
      481 taaacatttt atagttggag atgacaagtg gagaagagag tggacgaaca atccgttgta
      541 caattccgat catggcttga agtggagata tatcgaaaat ctctttactg aaattgtcca
      601 atatatggat agtaagttga gcggattaag tttacatgtc cgctatcgct atttaatgga
      661 tttgatgcat acccttgggg cggcattgtt cggtatttat ctggggtacc tgggatatat
      721 tttacttaag gtgaaaacag acgacatatc cctttcgatc attttatcca ttagcattgt
      781 ctgcctgttt ttcctgatga tattgctgga aatggaagac aaggtgaaaa agggtaaagt
      841 gaagcccgtc cccaaagata aaattcacta ccttgttgtg acgcccatta agagatttga
      901 tctgtttcaa ttatcaaacc cttctgcaaa ttaccttctt ttccttttta cccttttgta
      961 tatgggaagc ccatccccat atacagttac atacacgcct tcacattttg tgtttcgaat
     1021 tgctgttgta gttgctgttg ttctagcgtg gcttataccc aaattagcct acccgaagcg
     1081 ggttatcatt tccgaaatac tcacgctcgc cgtgctggag atattgtcgg ttatggcatc
     1141 taaatatatt aatgactgga gtgcctttaa gcctgattgg tggaatattg gctggtgtat
     1201 tctctttttt cttgtaacaa atatgatctt cataaaaaac cgtcaaaaca cacgtgatga
     1261 ccttatcgcg atgcaaaatt acacaatcaa tcgttacttt tccaacagta tacagccatt
     1321 tgaacctccc caaaaaacca gcggcataaa aagaaaaacg ccgaaattaa aaaggcgcaa
     1381 aaaataaagt tttggaaagg ataaaaaata ccttgttgcc gccccgcttc attatcagtc
     1441 gggtaaaata agacaaaatt ctcaaggatt cctcatgacc tacgaacaac aatttctcga
     1501 tgcactcaaa tccatcttta tcggcgcgaa agtggaaggc gattcaggtt acatcaacct
     1561 gatgaagatc aaagccagct actttgaaaa gggcgtcttc cctgttctga tgaaggacat
     1621 tgacaccgct tgcaagccgt ttgagaaggg cttccgcgag gagatgttcg ataag
//
LOCUS       DAOQMC010000368         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_55388_length_1658_cov_5.039925, whole genome shotgun sequence.
ACCESSION   DAOQMC010000368 DAOQMC010000000
VERSION     DAOQMC010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55388_length_1658_cov_5.039925"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PKV31_10380"
     CDS             complement(<1..1055)
                     /locus_tag="PKV31_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB93310.1"
                     /translation="MKDKMPYKVALVANSTWNFFNFRLNIIRSLKERGYEVVLISPPD
                     QYLAHLLEVVEVKHIPLNSLDRKSVNPFGEMALYREFKRIYRNEAPDLILHYTIKPNI
                     YGNMAAKKLGIHSICVITGLGYVFLHDNIYNSVAKRLYRKSLHKADLVVFENAEDKDL
                     FVQLALAPAEKTSVVNGCGINLEYFKPISYPEKSSAKTVFTFIARLILDKGIMEFIEA
                     AKSVRSRYPDVEFWVAGEIDEGNPTSISHETLYEWVESGVIKYFGHVRDVRPIIAKSD
                     CIVLPSYREAMARTLTEALAMEKPVISCDTGGCRTIVDDGVNGYLVPVKDVDALVHAF
                     ETFKLLPEISREKMGKSG"
     gene            complement(1058..>1658)
                     /locus_tag="PKV31_10385"
     CDS             complement(1058..>1658)
                     /locus_tag="PKV31_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB93311.1"
                     /translation="NYNKKCTTGHIHKVISFREKPDLATAEQFIQSGDYLWNAGMFFM
                     SIRTLLHNYKLHAPALYDMLTPFLSAGEEKVPGLIKELYPATPNLSIDYAIIEKAEDV
                     YTIPSDIGWSDLGTWNSLFQQVEKDDSGNAVMCNHIILENVSNTLVRNNTDKLVILRD
                     VKDLIIVEEEDVLLIYPMNKEQEIKQLGDKVRAKFEQYL"
BASE COUNT          440 a          393 c          344 g          481 t
ORIGIN      
        1 ccacttttac ccattttttc acgactgatc tcggggagca atttgaacgt ttcaaatgca
       61 tggaccaaag catcaacatc cttgacaggc actaaatatc catttactcc gtcatctaca
      121 atcgttctgc aaccaccggt gtcgcaggaa atgacaggct tttccattgc caatgcttct
      181 gtcagtgtcc gggccatcgc ctcccggtat gagggaagga ctatgcaatc tgatttcgca
      241 ataatcggcc ttacatccct gacatggcca aaatatttga tgacgcccga ctcaacccat
      301 tcgtagagtg tctcgtggct aatcgatgtt ggatttcctt catcgatttc accagccacc
      361 caaaactcga catccggata gcggctccta acagactttg ccgcctcaat gaattccatg
      421 atacctttat ccaggatcaa ccgcgcgata aaggtaaata cggtcttggc agaagacttc
      481 tccggatagc tgataggttt aaaatattcc aggttgattc cacaaccgtt gaccaccgat
      541 gtcttttctg ccggagccaa tgccagctgc acaaagagat ccttatcctc tgcattttca
      601 aatacgacga ggtccgcctt gtgcagggat ttacgataaa gtctttttgc cacactattg
      661 taaatattgt catgcaaaaa gacgtatccc agcccggtta tgacgcaaat cgagtggatg
      721 ccgagtttct tcgccgccat attgccataa atgtttggct taatggtgta gtgcaagatg
      781 agatcaggag cctcgtttcg gtaaattctt ttgaattcgc gatacagagc catctcacca
      841 aaaggattga cactttttct atccagacta ttgaggggaa tatgcttcac ctccactact
      901 tcaagcagat gggccagata ctggtcaggg ggtgatatca gcaccacttc atagccacgt
      961 tccttcaagg atcgaataat attcaaccgg aaattgaaaa aattccaggt agaattggct
     1021 actaaggcaa ccttgtatgg cattttatct ttcaatttta taaatattgt tcaaacttag
     1081 cacgaacctt gtcgcctaat tgtttgattt cctgttcttt attcattgga taaatcaaca
     1141 atacgtcttc ctcttccaca ataatcaaat ccttgacatc ccttaaaatt accaatttat
     1201 ccgtattgtt ccttactaaa gtgttggaga cgttctccag aatgatgtga ttgcacatga
     1261 cggcattgcc cgagtcatcc ttttcaacct gttgaaacag agagttccag gtacccaggt
     1321 ctgaccagcc aatgtctgac ggtatggtat agacatcttc cgccttctct atgatggcat
     1381 agtcgatgga cagattgggt gtagccggat aaagttcttt gattaaaccg ggcaccttct
     1441 cttcccctgc tgacaggaaa ggtgtcaaca tgtcgtaaag agcgggtgca tgaagtttgt
     1501 aattatgcaa caaagttctg atgctcataa aaaacatgcc ggcattccac aggtagtctc
     1561 ccgattggat aaactgttcg gcagtggcaa ggtcgggttt ctcccggaag gaaatgactt
     1621 tgtggatgtg acctgtcgtg cattttttgt tgtagttg
//
LOCUS       DAOQMC010000369         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_56729_length_1638_cov_4.703095, whole genome shotgun sequence.
ACCESSION   DAOQMC010000369 DAOQMC010000000
VERSION     DAOQMC010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56729_length_1638_cov_4.703095"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKV31_10390"
     CDS             complement(<1..660)
                     /locus_tag="PKV31_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNB93312.1"
                     /translation="MTIKIQHICLQIILVISSYFQVFGQTTNNRIEIFFTRPITARYQ
                     NPNLFPYGSGGSVTKQKIIDEIKNAKSTVDVCVYNNTENDIVDALKLVKAKGVRIRYI
                     TDSGTGNSALKGNPGFPILYLGKNGGIMHNKFVIIDAANTSATLLFGSANFTPNAFND
                     DANNLLVMRDQSICETFTTEFEEMWGGSGATPGPTSKSGNQKNDNTPHEFNIAGIPIN
                     VY"
     gene            complement(677..>1638)
                     /locus_tag="PKV31_10395"
     CDS             complement(677..>1638)
                     /locus_tag="PKV31_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNB93313.1"
                     /translation="NLSMYGGYEVAGGEYRINLIPLYNLSIFGAKFLIKKGGTLIWNG
                     DPINAQVNIDADFKGVNTSPYNFISEYIPEGDTKLQSEANRPTPVDLTLNLRGDLLKP
                     EITFKIDFPQLSPDLRTYVDSKLRSLEQDPNELYKQAASLVSFGSFIPKDNFNLSAVT
                     ATAYNTLSDLISSQLTQILSPMLTAAVADGKILTGVDLNLNYNFYSANTSQGTNIQNR
                     IGSELLIGPSLKFFDGRLIVNTGVKSGEQGKNQNYVAGDVDLQYYLTTDRKYVLRLYQ
                     NNDAVLEGRRIKSGVGISFQKSMDSIAELFRREQKKKHTKTRD"
BASE COUNT          440 a          320 c          340 g          538 t
ORIGIN      
        1 gtaaacatta atggggatac ctgctatatt gaattcatga ggtgtattgt cgtttttctg
       61 gtttcctgac ttactcgttg ggccgggggt ggcacctgat ccgccccaca tctcctcaaa
      121 ctcagtagta aatgtttcac aaatgctttg gtctctcatg acaagaaggt tgttggcatc
      181 atcattgaat gcatttggtg taaaattagc agatccgaaa agtaacgtgg ccgatgtatt
      241 tgcggcatca atgatgacaa atttgttgtg catgattcca ccattttttc cgaggtaaag
      301 tatcggaaaa ccgggattgc ccttaagagc tgagttgcca gttccggaat ctgttatata
      361 tctgatcctt actccttttg ctttgacgag ttttaaagca tctactatat cattttcagt
      421 attattgtaa acacacacat cgacggttga tttggcattt ttgatttcat caattatttt
      481 ttgcttggtg acagatcccc cggagccata tggaaacaaa ttgggattct ggtaccttgc
      541 agtaatgggc cgggtaaaaa atatttcaat cctgttattt gtcgtttgcc cgaatacttg
      601 aaagtaagaa gagattacca atataatttg aaggcatata tgctgaatct ttatggtcat
      661 tgcttgcatt aaaaaattaa tctctggttt tggtgtgttt tttcttttgt tctcttctga
      721 ataattctgc aattgaatcc atagattttt ggaaggagat tccgacccca ctcttgatac
      781 gacgcccttc caatactgca tcattgttct gataaagacg aagaacatat tttctatcag
      841 ttgtcagata gtattgcagg tcaacatcac ctgcgacata attttggttt ttgccctgct
      901 caccactttt tacgcctgta ttgacaatga ggcgaccatc gaagaacttc agactgggac
      961 ctataagtaa ttcgcttcct atcctgttct gaatattagt tccctggctt gtattggctg
     1021 aataaaaatt gtaattgagg ttaaggtcca ctccggtcaa tatttttccg tctgctaccg
     1081 ctgcagtcag cattggagaa aggatttgtg tcaattggga ggaaataagg tcagaaagag
     1141 tgttgtatgc agttgcagta actgccgaca ggttgaagtt gtccttgggt ataaatgaac
     1201 caaaagaaac caacgatgcc gcctgtttat acaattcatt tggatcctgt tcgagcgaac
     1261 gcaatttgct atctacatag gttcttaggt caggggaaag ttgagggaag tctattttaa
     1321 aggttatttc cggtttcagt aagtctcccc taaggttcaa agtaagatcc accggggtag
     1381 gccgattggc ttcactctgc aattttgtgt caccttcagg tatatattca ctgataaaat
     1441 tatacggtga agtattgacc cccttaaaat cggcatcgat attgacctga gcattgattg
     1501 ggtcgccatt ccatatcagc gttccccctt ttttaatcaa gaattttgca ccaaaaatag
     1561 aaagattgta cagggggata agattgattc tatactcacc gccggctact tcatatcctc
     1621 catacatgct aagattgc
//
LOCUS       DAOQMC010000370         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_57174_length_1631_cov_6.958122, whole genome shotgun sequence.
ACCESSION   DAOQMC010000370 DAOQMC010000000
VERSION     DAOQMC010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57174_length_1631_cov_6.958122"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1631
                     /locus_tag="PKV31_10400"
     CDS             <1..>1631
                     /locus_tag="PKV31_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNB93314.1"
                     /translation="QSIGIEVDLPAGFDADNSTYCTIKYKPADTGTWKDAFPPDRITF
                     SGQDQFRGSVFLLQAGTPCDIAVSIHDSIPEEKVIYQHTVSTSTLNDPETIVGTEIRW
                     VAPDGSGKEYTKDKPGQLKTLLESGDVTCGTTIYLMDGVYTDLSIVFRLNSSCTEEKP
                     IHIMAAPGAHPIIDGAYQGTLAWTRNEHDTLLYRSNLPPNTGYTNICILNGNNLYPYP
                     TLNAFWYFHDHNLRDLNFNADGFVRDESNIWIKTKAGTNPNNTDVTISKAFRFITVYG
                     NSHDGYLHFKGLTIKNIAKNQKESQSMAFDLRDVNHIWFDSCQFVFNNFDIQFGGKCD
                     HILIENCQFKNDNGLYSHVMIKRSPLYFITEATSYARNRETGALVFKNNSNVTIRNNV
                     FTGTNSGMYLNSSAGVAEADVYDNEFVDNYDAIECDGNWVNFRVLNNRFVRPMAGISA
                     APPGIGPRYFYRNVFYGMRGRENVKDDPYFVGCTPPTVYYSPATGIKTNSSGTNPARG
                     NMYFFNNTFYSDDTLAFTMEPWLGEWKNIYFVNNI"
BASE COUNT          475 a          349 c          366 g          441 t
ORIGIN      
        1 atcaaagtat cggtattgaa gttgatttgc cggcaggttt tgatgccgat aattcgacct
       61 attgtaccat aaaatataag ccggccgata caggaacctg gaaggatgca tttcccccgg
      121 atcgaattac tttttccggt caggatcaat tcaggggaag tgtgtttttg ttgcaggccg
      181 gcaccccttg cgatatagcg gtgagtatcc acgatagtat tcctgaggaa aaagttatat
      241 atcaacacac tgtaagtacc tcgaccctca atgatcccga gacaattgtg ggtacggaaa
      301 taagatgggt cgctccggac ggttccggca aagaatatac aaaggataag ccgggacaac
      361 taaagacgtt gctggaatcg ggggatgtaa cctgtggcac gaccatttat ttaatggacg
      421 gcgtatatac tgatctttcg atcgtttttc gattgaacag ctcctgtacc gaagaaaaac
      481 cgatccatat tatggcagct ccgggcgcgc atcccattat cgatggcgcc tatcagggca
      541 cgcttgcctg gacaaggaat gaacatgaca cgcttctata ccgctctaat ttacctccca
      601 acacaggata taccaacatc tgtatcctca atggcaacaa cctgtatcca tatcccaccc
      661 tgaatgcatt ctggtatttt cacgatcaca atttaagaga tctgaatttt aatgcagatg
      721 gatttgtaag ggatgaaagt aatatctgga tcaagacaaa ggccggaaca aaccccaaca
      781 acacagacgt cactatctca aaggcatttc ggtttattac ggtctatggc aacagccatg
      841 acgggtacct tcattttaaa ggattgacga ttaaaaatat cgctaagaat caaaaggaaa
      901 gtcagtctat ggcatttgat ctcagggatg tcaatcatat ttggtttgac tcctgtcaat
      961 ttgttttcaa taattttgat atccagtttg ggggcaagtg cgatcatatt ttgattgaaa
     1021 attgtcagtt taaaaatgac aatggcttgt acagtcatgt aatgatcaaa cgatctcccc
     1081 tgtacttcat cacagaagcc acttcctatg caagaaacag ggagaccgga gccctcgtat
     1141 tcaagaacaa ttcaaatgtt accatccgga ataatgtatt cacggggacc aatagtggca
     1201 tgtatctcaa cagcagcgca ggtgtagctg aggctgatgt ctatgacaat gaatttgtcg
     1261 acaactatga cgctatagag tgtgatggca actgggtcaa tttccgggtg ctgaataata
     1321 gatttgtacg tccgatggcc ggtattagtg cggcaccgcc tggtattgga ccccgctatt
     1381 tttacaggaa tgtattctat ggcatgcggg gccgggaaaa tgtaaaggat gatccttact
     1441 ttgtgggttg taccccgccc acagtgtatt attcaccggc aacaggtata aagaccaact
     1501 cttcaggaac aaatccggcc agaggaaata tgtatttttt caacaatact ttttactctg
     1561 acgacaccct ggccttcacg atggagccct ggttgggcga atggaaaaac atctacttcg
     1621 ttaataatat t
//
LOCUS       DAOQMC010000371         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_59055_length_1603_cov_5.414729, whole genome shotgun sequence.
ACCESSION   DAOQMC010000371 DAOQMC010000000
VERSION     DAOQMC010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59055_length_1603_cov_5.414729"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PKV31_10405"
     CDS             <1..1007
                     /locus_tag="PKV31_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB93315.1"
                     /translation="GHATSLYSGVIKSIPVQIGDYIRKGQVIATITNPQFIQLQEEYL
                     TLNSKIILAEQELQRQNELNQENAGTLRNLQSATAELNTLKTRKASLTEQIKMMGINP
                     ATITNQSLRSVLSVTSPISGTVSKLYGKIGSYVDVSSPVAEIVDNSSLHLDLHVFERD
                     LGLLKVGQIIHFTITNNPVNEYDAVIYSIGTAFENDSKTIPVHAAVKGNKAGLIDGMN
                     ITGVVSLDKATTQAVPDGAIVESEGKFFIFIQTDKEPEDHQHEGEDDGHGHGIATVEK
                     KHLPTDMNFEKIEVAKGVSNMGYTALTLVKDIPADAKIVTKGAFFINARLTNAGEDGH
                     "
BASE COUNT          516 a          293 c          366 g          428 t
ORIGIN      
        1 agggccatgc aacgtcctta tacagtggtg tgataaagtc tattccggtt cagatagggg
       61 actatatcag gaaaggtcag gtaatcgcta ccatcaccaa tcctcagttt atccagttgc
      121 aggaagaata tctgacgttg aacagtaaaa ttattctggc tgaacaggaa ttacaacgtc
      181 agaatgaatt gaatcaggaa aatgccggta ctctcagaaa ccttcaaagc gcgacggcgg
      241 aattgaacac cttgaagacg cgaaaagctt ctttgacaga acaaataaag atgatgggaa
      301 tcaaccctgc taccatcacc aatcaatcct taagatcggt attgtccgtt acaagcccta
      361 tttcgggtac ggtcagtaaa ttatatggga agataggcag ctatgtcgat gtttcttctc
      421 ccgttgcaga gattgtggat aatagctcgc tgcaccttga cttacatgta tttgaaagag
      481 acctgggatt gttgaaagtt gggcagatca tccattttac aattacgaac aatccggtaa
      541 acgaatatga tgccgtgata tacagtatcg ggacagcgtt tgaaaatgac agcaaaacca
      601 ttccggtaca tgcagccgtc aaagggaata aggccggact tattgatggg atgaatatta
      661 ccggggtggt aagtcttgat aaagccacaa cccaggcagt ccctgatggt gctatcgttg
      721 aatccgaagg gaaatttttc atcttcatcc aaacggacaa ggagcctgaa gatcaccagc
      781 atgaaggtga agatgatgga catggacatg gcatagcaac tgtagagaag aagcatttac
      841 caaccgatat gaattttgaa aagatagagg tagccaaagg tgtatccaat atgggctata
      901 cagcattgac acttgtaaag gatattcctg ctgatgccaa aatagtcacc aaaggcgctt
      961 tttttatcaa tgccaggctc acgaatgccg gtgaagacgg acactgatga tacaatgaaa
     1021 atttccgggg aaggaaaagt taaatatctt gtcgattccc cgaaaattgg actgacaagg
     1081 gtgctgcttc tttgcaagtt gggtgtcgga ggagggacag attaatcata cagagattat
     1141 aggtggtcaa tcattaaaaa tacagttcaa attttatttt ggaaagacct ggaccaaaca
     1201 tcgatgatag aatagtttgc aaaatcaata atgggttgaa aatggatggc aatgaacttt
     1261 gtaaaacttt caaaaagatt attcccaatt gtgcatcatt ctgtaggaaa taactggggc
     1321 tattgtgaac ataaaatttg attgaaaagg ttgtcacttc cctgttcatt tattctcgca
     1381 tttgaaacat ccggataaca acaatccaat catcaggcaa gtcggtttgg ccggccatcc
     1441 aacttatttg ctgcattaat gacgaatggc atgatccaaa atattccggt tagctatatt
     1501 gacttaatgg tatgatggat ggtcttgaca ggacatatca ttacgcagtg ataatatacc
     1561 gactggtcag gtttttagtt tgcggaatga tagaatttat gca
//
LOCUS       DAOQMC010000372         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_61373_length_1572_cov_4.009888, whole genome shotgun sequence.
ACCESSION   DAOQMC010000372 DAOQMC010000000
VERSION     DAOQMC010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61373_length_1572_cov_4.009888"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKV31_10410"
     CDS             complement(<1..374)
                     /locus_tag="PKV31_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HNB93316.1"
                     /translation="MTKFASACRPAREVPAKLLYLMMNKVLKLDPKDNTLVALTNLHK
                     GETVILDGRETVLVDDIPAKHKFFEDNMKAGSEVYMYGVLVGKVQTDILKGMRMTVEN
                     TKHAADPYHYRPYHYQWRGPDV"
     gene            1081..>1572
                     /locus_tag="PKV31_10415"
     CDS             1081..>1572
                     /locus_tag="PKV31_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93317.1"
                     /translation="MMNRLFYFIVLIIFAAGFISCDFDEAGPPSSVRGYAPVYFPKTN
                     NDTLKFVGPQPTEKAGKIVLKGDLLYQVEQYKGIHIIDFRDANNAKKIGFYEIFGCTE
                     LTIKDHYMYTNSGTAFIVIDIADLFNPRVVDYRPDYWVGLSFPPPPGNGYFECPDPQK
                     GIVI"
BASE COUNT          479 a          325 c          337 g          431 t
ORIGIN      
        1 tcaacatcag gcccccgcca ttgatagtga taaggcctgt aatgatatgg gtcagcggca
       61 tgttttgtgt tttcgaccgt catgcgcatc cccttcagga tatccgtctg tactttgccc
      121 acaagaactc cgtacatata gacttcactt ccggctttca tgttgtcctc aaagaacttg
      181 tgttttgcag gaatatcatc caccaacacc gtctcacgac catccaggat gacggtttcg
      241 cctttgtgta ggttggtcaa tgccactaag gtattgtctt tagggtctaa tttcagaact
      301 ttattcatca tcaaataaag aagtttagcc ggaacttctc ttgcaggccg gcaggcagaa
      361 gcaaatttag tcaaaattaa tttctttaac agggggagaa tgaattacta agaaatgact
      421 tgctccgcag cttgccaaca ggccacaaca cgaccaacaa aagcaagtag gagcaaagca
      481 tagagacctt ctcacgccat tactcggggc gaacattcaa gccgcttaac cggattatga
      541 agcatttaaa ccggtgtttt tatgcacgac actcgtattt gaaatgaaaa tgacacagga
      601 acaacgtgca gaaaaaggtg ggacaactaa atcctacagt tgaaaggttg agaataattt
      661 aaaagaaggg cgaatttcga cacttaaaag aacattgaac ttggctttgg cggacgactt
      721 gaactgaata taaaaatttg acagattccc gaaacaaata aagaaaaagg gttaacccaa
      781 gctttgtgca acaatcaaaa tttgtatctg gcttgacagt gtatagcttc ggaatcgcca
      841 ccccgggcag ctgcaaaccg atgaaaggat gtcctaaagt atagtgaaat ggaccaacct
      901 gacttgatcg gatgtagagc ctcgaaataa gccgggcatt aatattttgt taaaaaataa
      961 tccggatgga taaaattttt ggataccaac caattagttg ctatcttcgt tagtaatatt
     1021 acatggtaaa tatttctgtg taatgggaca aaaaatcttg aaaccaatta tcattcaatt
     1081 atgatgaatc gcctattcta ttttattgtg ttgatcattt ttgccgccgg atttatttcc
     1141 tgtgacttcg atgaagcagg accgccgtca tcggttaggg gatatgcccc ggtgtatttc
     1201 ccgaagacca acaatgacac cttgaaattt gtcggtcctc agcctactga aaaagccgga
     1261 aagattgtgt tgaaaggaga tttgttgtac caggtggagc aatacaaggg catacacata
     1321 attgatttcc gggatgcaaa caatgccaag aagatcggtt tctatgaaat cttcggatgt
     1381 actgaactga ccatcaagga tcattatatg tacactaatt caggcacggc atttatcgtc
     1441 atagacattg cagacctgtt taatccgcgt gtggtagatt acaggccgga ttactgggtg
     1501 ggactttcat ttccgccacc tccgggcaat ggctatttcg agtgtcccga ccctcaaaaa
     1561 ggtattgtaa tt
//
LOCUS       DAOQMC010000373         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_62075_length_1563_cov_3.677056, whole genome shotgun sequence.
ACCESSION   DAOQMC010000373 DAOQMC010000000
VERSION     DAOQMC010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62075_length_1563_cov_3.677056"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PKV31_10420"
     CDS             <1..728
                     /locus_tag="PKV31_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNB93318.1"
                     /translation="IYLLSQSFDGHVGEHSNIQAFLNEIFSYLSAETKVKLEAPEMHM
                     NLDLLQDIDSNHIPKRVWDMVQKSVKGHRKLTFNYVSPTYEDRQKRLHQVVPIRIQYQ
                     SGHWYLRAHRLLRRDSSGKEDRQERHLKYRLSYIQDDEKLVISATIMPSPPEAEKYYV
                     HYRLLPPLSRGIISEHFYDMKITGLDDGSVEITGYCDDAWEAGRLLLSYGEYCVVLGG
                     DEVKAWMEKTIASLIKNYQYLGK"
     gene            1121..>1563
                     /locus_tag="PKV31_10425"
     CDS             1121..>1563
                     /locus_tag="PKV31_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93319.1"
                     /translation="MMFSESKPTPEQGQIVTVRQRRYVVLDVQPNTLPFQPLAIQQVP
                     GQNLITLNSIEDDALGEMLQVIWELEPGTNIEEKVGLPQLDGFDAPERLDAFMNAVRW
                     GAISSADTRALQAPFRSGIEIEDYQLDPLVRAIQMPRVNLLIADDV"
BASE COUNT          470 a          326 c          362 g          405 t
ORIGIN      
        1 ccatttatct actttctcaa tccttcgatg gccatgttgg agaacattcc aacattcagg
       61 cctttctgaa tgagattttc tcctatcttt cagcagaaac aaaagtaaag cttgaagcgc
      121 cagaaatgca tatgaatctg gatctgcttc aagatatcga ttcgaatcac ataccgaaaa
      181 gagtctggga catggttcag aaatccgtta aaggacatcg taaattgacc tttaactacg
      241 tatcccccac ttatgaagat cgccagaaac gcctgcacca ggtcgtacca attcgcatcc
      301 agtatcaatc ggggcattgg tatctacggg cacaccgact gcttcgtcgt gactcaagcg
      361 gaaaagaaga tcgacaggaa agacacctca agtacagatt aagttatata caggacgatg
      421 aaaaactggt tatatcggcc accattatgc catcgccgcc agaagcggaa aaatattatg
      481 ttcattaccg attacttcca ccattatcga gaggaatcat cagcgagcat ttttacgaca
      541 tgaagatcac aggtctggat gatggcagtg ttgagattac gggatattgc gatgatgcat
      601 gggaggcagg acgtttgttg ttgtcatacg gtgaatattg tgttgtatta ggaggagatg
      661 aagtaaaggc atggatggaa aaaacaattg caagtttgat aaaaaattat caatacctgg
      721 gaaaatagag tagcccgcaa gtatgggctt gcccgccgtg tatgataagc aaagttacat
      781 cttgcttatc aggcccagca tccacctgga ggggaacatg ctgggcaggg gtttgtaaat
      841 attattggat caacttgtaa tccaattgaa agtaccactc cggttgtcta ggctttacca
      901 taaacctcac agggctgtat aattgaagag gtctgaataa aagctacgtg attttaggcg
      961 taacggatgg ggtaatgaat agcatattcc caccgccaac ccaaattttg tgttggcgtt
     1021 ttttattgga caggcataaa caaaggaaaa ataatacggc attggtctaa aaaggaattc
     1081 ggcttatcaa ataaatatca tatctgtcgc acaaggtgcc atgatgtttt ctgaatccaa
     1141 accaacccca gaacaaggtc agatagttac agttcgtcaa agacgctatg ttgtcttgga
     1201 tgtgcagcca aatacactgc cgtttcaacc gctcgctatt caacaagtac cgggacaaaa
     1261 cctgattacg cttaattcca ttgaggatga tgccttggga gaaatgcttc aggtgatttg
     1321 ggagttggag cccgggacaa acatcgagga aaaggtgggc ctcccccaac ttgacgggtt
     1381 cgatgcaccc gaacggttgg atgctttcat gaatgcagtt cgctgggggg cgatttcatc
     1441 agccgataca cgcgcattgc aagccccttt ccgcagcgga attgaaattg aagattatca
     1501 acttgatcca ttggtacgcg ccattcaaat gccgcgcgtt aatttattaa ttgcagacga
     1561 tgt
//
LOCUS       DAOQMC010000374         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_62209_length_1562_cov_2.317850, whole genome shotgun sequence.
ACCESSION   DAOQMC010000374 DAOQMC010000000
VERSION     DAOQMC010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62209_length_1562_cov_2.317850"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PKV31_10430"
     CDS             <1..644
                     /locus_tag="PKV31_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93320.1"
                     /translation="YEDTFQTMMNSRYVIVVLDPTYISRKETQLDINLENKQGKEIDV
                     EENDLDDYDMDDDMPELGRKNTFTREEYTQIIHYLLMTLSKNSPQKRYLAMCITKMDK
                     MRIQGSPWTLLKRLFGQEMFIVLENYKKLFDIEVFVTSAAGFVKDKKDNMGVKVSNEV
                     DGDIKDISQWQPVNTAAPFFWIFENREKEKIKSSGNSFSNNLRYYIEYPRRNF"
     gene            971..1366
                     /locus_tag="PKV31_10435"
     CDS             971..1366
                     /locus_tag="PKV31_10435"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNB93321.1"
                     /translation="MTWLLFYLLAVSLYDLRTRRIPNWSTYPLILAGMIAHFPGHMDL
                     WLACFLLLSTWANGWMGAGDVKLWMAVLWALPDSNTPSLVLLVFLSFLITSIIQFFWR
                     LFQKQSLTGMKSPAAWRTIPFLLMVWHVH"
     gene            1356..>1562
                     /locus_tag="PKV31_10440"
     CDS             1356..>1562
                     /locus_tag="PKV31_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93322.1"
                     /translation="MYTDLFLAMLNPKNARGNPILSALLYSFCPRAAGWWLTGVDPTP
                     PFDPVWKSLEDLSTGKTLAEFLIQY"
BASE COUNT          452 a          358 c          298 g          454 t
ORIGIN      
        1 gttatgaaga tacatttcag actatgatga attcgcgata tgttattgta gtacttgatc
       61 caacatatat ctcacgcaaa gaaactcaac tagatattaa tctagaaaat aaacagggaa
      121 aggaaattga tgtcgaggaa aacgacctcg acgattatga tatggatgat gatatgccag
      181 agcttggcag gaaaaataca tttacacggg aagaatatac acaaattatt cactatctat
      241 taatgacact atctaaaaat tcgcctcaaa agcgatacct tgctatgtgc attacaaaaa
      301 tggataaaat gcgtattcaa ggcagtccat ggacattact taaaagactg tttggtcaag
      361 aaatgtttat agtgttggag aattacaaaa aactttttga cattgaagtc tttgtcacat
      421 ccgcagcagg ctttgttaag gacaaaaaag acaatatggg cgtaaaagta tcaaatgaag
      481 ttgacgggga tattaaagat atatcacagt ggcaaccggt aaatacagcg gcacctttct
      541 tttggatttt tgaaaatagg gagaaggaaa aaatcaagtc cagcggcaat tcctttagta
      601 ataatttaag atattacatt gaatatccta gaaggaattt ttaaatttgg cttgaaattt
      661 atttccgaat tgcttaccaa aaacttcccc actaacccac caactcactg aagggcagcg
      721 atggtgtcat atcgccctgc ctacctgaat tccacgtcac tatcatctca cgctttgcac
      781 gcgtgatacc cgcatagaac agatgcaggc gtttcttcac atataaccat ctacaaatct
      841 ttactcggca tattcctaat aattaactga tctacctcgt caaagtttct ctgtcaaaaa
      901 gatttgtatt cgctttttta tcgacacatt ccacacaccc ctcagcttat tcatatatca
      961 tcagcaacca atgacctggt tgctttttta tcttctggca gtaagcctgt acgatctgcg
     1021 tacccgtcgc atcccgaact ggtctaccta tccgttgatc cttgcgggta tgatcgcgca
     1081 tttccccggg catatggatt tatggctggc atgttttctg ctgctttcca cctgggcaaa
     1141 cggatggatg ggcgctggag atgtcaaact gtggatggca gttctttggg ctttacccga
     1201 ttccaatacg ccatccctgg tcctgcttgt attcctttca ttcctgatca ccagcatcat
     1261 tcagttcttc tggagactct ttcagaagca atcccttacc ggcatgaaat ccccagccgc
     1321 ctggcgaaca attccctttc tcctgatggt ctggcatgta cactgatctt ttccttgcca
     1381 tgctcaatcc caaaaacgca cgcggtaatc cgatcctgtc cgcgcttttg tattcatttt
     1441 gtcccagagc ggcaggttgg tggctgacgg gtgtagaccc cacaccacct tttgatcctg
     1501 tctggaaatc cctggaagat ctatccaccg gaaaaaccct ggcagaattc ctgatccaat
     1561 at
//
LOCUS       DAOQMC010000375         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_63409_length_1546_cov_3.533199, whole genome shotgun sequence.
ACCESSION   DAOQMC010000375 DAOQMC010000000
VERSION     DAOQMC010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63409_length_1546_cov_3.533199"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..1081)
                     /gene="pseF"
                     /locus_tag="PKV31_10445"
     CDS             complement(389..1081)
                     /gene="pseF"
                     /locus_tag="PKV31_10445"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HNB93323.1"
                     /translation="MARLAIIPARGGSKRIPGKNIRNFHGKPIIAYSIEAARQSGLFD
                     EVIVSTDDDQIWDIAVQYGASVPFLRSAENSNDTATTLDVIREVLAALKHQGKEYQEV
                     CCIYATAPLIRISALEEGLRRLDEVTASVFPVVAFGFPIWRSLKLDADQRVSMNWPEF
                     SQTRSQDLPPAYHDAGQWYWFRPDLLTDSLYAPSSAAIFISEMEAQDIDNESDWKLAE
                     IKYSLLQKVREA"
     gene            complement(1074..>1546)
                     /locus_tag="PKV31_10450"
     CDS             complement(1074..>1546)
                     /locus_tag="PKV31_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNB93324.1"
                     /translation="DTYEYPGWYMELQQLGYNYRLTDIQAALGISQLKRADAGLERRR
                     EIAGTYNTFFSGKPYLIRQSGSVEGHAYHLYVVEVEDRLGLYNHLREHNIFAQIHYIP
                     VHLMPYYRQFGWKEGDLPHAEEYYRHCISLPMYPTLTADEQTFVMDIIDKYYLG"
BASE COUNT          405 a          371 c          304 g          466 t
ORIGIN      
        1 cgcgttaaaa ttaacatgct accataaggt cgcttctaac gaagctttat attccgattg
       61 tttttatcat ccggaattct aaatatctcc atcgtttatt tcaattttac ggattgaaag
      121 cgctgtaggc gcaacctttt ggtttcaatt ttgagcccat gaagttcggg attcataatt
      181 tgtagaatat taaaccgaat gttgtttttt tgaaccatta aggatataag attattttcg
      241 tctctcagct cttatctttc cttatttgct taatggttga atcaaaaatt ttgagttttg
      301 agttaaatcc aactaagaac taaaaactaa aaactaagaa ctttacaact ttccaattta
      361 cacattatcc gttgatagcg aaatattctt acgcttccct gactttctgc aggagggaat
      421 actttatctc tgctaacttc cagtcacttt cattatcgat gtcctgagct tccatttcac
      481 tgatgaaaat ggccgctgat gacggtgcat acaggctgtc cgttaaaagg tcggggcgaa
      541 accagtacca ttgtccggcg tcgtggtaag caggtggcag gtcctgactc cttgtctgac
      601 taaattcagg ccagttcatg gatacgcgct gatcggcatc cagcttcaat gacctccata
      661 tcgggaatcc gaaggcaact accggaaaca cactggctgt cacctcatcc agccgcctca
      721 acccttcctc caacgcgctg atacggatca agggcgcagt ggcatagata cagcatactt
      781 cctgatattc ttttccctga tgcttcaacg ctgcaagcac ctcccggata acatcgagtg
      841 tagtggcggt atcattgcta ttctcagcag aacgtaagaa tggtacagag gcaccgtatt
      901 gtactgctat atcccagatc tgatcatcat ccgtggaaac tattacctca tcaaagaggc
      961 cgctctgcct tgccgcctca atggaatagg caattatcgg ctttccgtga aaattcctga
     1021 tattttttcc gggtattctt ttactaccac cccttgccgg tataatcgcc agtctagcca
     1081 aggtaatatt tgtctataat gtccatcaca aaagtttgtt cgtcagcagt cagtgtcgga
     1141 tacattggca ggctgataca atggcgataa tactcctcag catggggcaa atcgccctcc
     1201 ttccacccaa actgtcggta atacggcatc agatgaaccg ggatataatg tatctgtgca
     1261 aaaatattgt gttctcgtaa gtgattatac aagcccagcc ggtcttcaac ttcgaccaca
     1321 tacaggtgat aggcatgacc ttcgaccgaa ccggattgtc tgatgagata aggctttccg
     1381 gagaaaaagg tattgtatgt ccctgcaatt tccctccttc tttccagccc tgcgtctgct
     1441 ctttttaact ggctgattcc cagtgctgcc tgtatatctg tcaaccgata gttatagccc
     1501 aattgttgca attccatata ccatccggga tattcgtaag tatcag
//
LOCUS       DAOQMC010000376         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_63654_length_1543_cov_4.819892, whole genome shotgun sequence.
ACCESSION   DAOQMC010000376 DAOQMC010000000
VERSION     DAOQMC010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63654_length_1543_cov_4.819892"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1543
                     /gene="pheT"
                     /locus_tag="PKV31_10455"
     CDS             <1..>1543
                     /gene="pheT"
                     /locus_tag="PKV31_10455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNB93325.1"
                     /translation="EVIEIGLTPNRADANSHYGTARDLRAALHLHKGYNQALNRVKTL
                     DTAGLPTSDFKITIENEVACKRYTGILIEQVKVGPSPDWMKTRLEAIGQRSVNNIVDI
                     TNYVMFELGQPLHAFDADKIAGRHIRVKNVAAGTPFLTLDGSSKNLDADDLLICDGNG
                     DPMCIAGVYGGKDSGVTDTTVNIFLESAWFNPASVRRTSFRHNLRTESASHFEKGTDP
                     EQCDEALLRAAYLMKEYAEAEVRFTFLDCYPGKSERTKVNINLPRVRTLIGKHLTAQE
                     ISATLTSLNIDIEEKDTDNWQVTIPSHKTDVSRQADVVEEIVRIYGLNNIEETGSFNF
                     SMPDKVSNEDIEARHTVLQYLADAGYSEAMGLSIINSNNWAKITDNPDIKGIRINNTS
                     NVQLDLMRPDPLATALETIAYNQSRQQSDIRMYEFGSIYDIDQKGYLEATFISLFGTG
                     SLHEESWHIGKRKNLDPYYFKTTIQHLLKRIGIRKYQERNGTDERFENCLEIVLGPKV
                     LAVTGN"
BASE COUNT          492 a          328 c          351 g          372 t
ORIGIN      
        1 cgaagtcata gaaattggcc tgacacccaa ccgggctgat gccaactctc attacggcac
       61 tgcgagggat ctccgggctg cacttcacct gcataaagga tacaatcaag cgctcaatcg
      121 cgtcaaaacc cttgacaccg caggcctccc cacttctgat tttaagataa ccatcgaaaa
      181 tgaagttgct tgcaagagat ataccggaat cttgatcgaa caggtcaagg taggtccttc
      241 gccggattgg atgaaaacaa gactggaagc cattggtcag cggtcagtca acaatatcgt
      301 ggacattacc aactatgtga tgttcgaact tggccaaccg ctgcacgcat tcgatgcaga
      361 taagatagca ggacggcaca taagagtaaa gaatgttgca gccggcactc cctttttgac
      421 ccttgatggg agttcaaaaa atctggatgc cgatgacttg ttgatctgtg atggcaacgg
      481 tgatcccatg tgtattgcag gagtgtatgg tggaaaggat tccggtgtaa cagatacgac
      541 tgtcaatatt ttcctggaat cagcctggtt caatccggct tctgtccgcc gtacttcgtt
      601 cagacataac ctgagaactg aatctgcatc tcatttcgaa aaaggaaccg atcctgaaca
      661 atgtgacgaa gcattactta gagccgcata tcttatgaag gaatatgctg aagctgaagt
      721 acgttttacg tttttggatt gttatcccgg caagtcagag agaacaaaag tcaatatcaa
      781 tttaccgaga gtaaggacac tgatcggcaa acatttaact gcgcaggaaa tcagcgccac
      841 actaacatct ctgaacatcg atatcgaaga aaaagataca gataactggc aggtaaccat
      901 tccttcgcat aagacagatg taagcaggca agccgatgtc gtagaagaaa ttgtcagaat
      961 ttatggactg aataatatag aagaaacagg aagttttaac ttttccatgc cggacaaagt
     1021 ctcaaatgaa gatatagagg caaggcacac ggtgcttcaa tatttggcag atgccggata
     1081 cagtgaagca atgggattat ccattatcaa cagtaataat tgggcaaaaa taaccgacaa
     1141 tccggatatt aagggcatca gaatcaacaa tacttctaat gtccaacttg acctaatgag
     1201 acccgaccct ctggcaactg ctcttgagac aatcgcatac aatcaatcca gacaacagtc
     1261 ggatatccgg atgtatgaat ttggaagtat ttatgacatc gaccagaaag gttaccttga
     1321 ggcaaccttt atatcgttgt ttggaaccgg gtccttgcat gaggaatcct ggcatattgg
     1381 caagcgaaaa aacctggatc cttattattt caagacaacg attcagcatt tgctgaaaag
     1441 gattggtatc cggaaatatc aggagagaaa cggaaccgat gaaaggtttg aaaactgtct
     1501 cgaaatagta ttgggaccca aagtactggc tgtaaccggc aat
//
LOCUS       DAOQMC010000377         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_64730_length_1530_cov_5.185763, whole genome shotgun sequence.
ACCESSION   DAOQMC010000377 DAOQMC010000000
VERSION     DAOQMC010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64730_length_1530_cov_5.185763"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            596..>1530
                     /locus_tag="PKV31_10460"
     CDS             596..>1530
                     /locus_tag="PKV31_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93326.1"
                     /translation="MKTLIVFFSAILFFVVCFFPLGCKKDSLGVDKPSDSFQISERSE
                     SLNEFYSGGTTEEDTLTKLTVLGNVRNNPYTVQNFTAAYNELYEPDIQSLPVTHHYIK
                     FLPANQEEMIVLAKSDLNLYEYPLDREVIEMGDYYKEPGSPKDQISPFYAVVTPSQAL
                     PNLSYEILSELHLRSNDEDLVRRALERVGYDPDVVGYILPGEPGDNGGSGGSEFEINS
                     CGCHVYKEQRMPGGCIQVKDVEKDIFQGVRRVKVILKDTWFTEDETWTDDNGCFRINN
                     KYYGKAWMWVKFKSPRVQIRGARSGFAAVWEWAFPV"
BASE COUNT          453 a          270 c          358 g          449 t
ORIGIN      
        1 gctaaaaaac ttgccatggc atacaaaaca catagtgtca cgacgacgat gacgattaac
       61 tccatccggt ttcgacagct ctgcatgtaa attttccagg aatcctgatg atgctggttc
      121 atccgtatga ataattttta aaattcatgt gaaagtacat acaagcctct tgaaatcaaa
      181 atttttttga ataacatgtc agtcaatatg ccggacacgt aaggttctgg tggtgttcca
      241 ggttatttca aacaacgcat tgcctgttct gcagccatgc agccattaga catttcattc
      301 ctaacgccat catcggttag tgggttgatg tagttgcagc atcaccaagt agcatataat
      361 tgtcacccga aattttctcg agctaagaac ccaatggtag aggacgcctt ttatacggct
      421 ggtaaaatta gcttggcgga atctttggct aaatggtgga tttgatattt atggaataat
      481 ttgcagtggg tatcattgca aaaatatctg aagaaataat ataataaagt gttgatttat
      541 ggaaatgata gtattatatt tgtcggacaa atttggttaa ataattaaaa atattatgaa
      601 aacgttaatt gtattttttt ctgcaatttt attctttgtc gtatgtttct ttccgcttgg
      661 gtgtaagaaa gattctttag gagttgacaa accgtcagat tcgtttcaga tatctgaaag
      721 atcggaaagc ttgaacgaat tttattccgg agggacaaca gaagaggaca ccctgaccaa
      781 actgaccgta ttgggcaatg tgagaaacaa cccctatact gtccagaatt ttactgccgc
      841 ttacaatgaa ctgtatgagc cagacataca atccttgcca gtaacgcacc attacattaa
      901 gtttttgcca gccaatcaag aggaaatgat agtattggcg aaaagcgatc tgaatctgta
      961 tgaatatcct ttggacaggg aagtaattga aatgggagat tattataagg aaccaggtag
     1021 ccctaaagat caaatttcgc cattctatgc ggttgtaacc ccgtctcagg cactcccgaa
     1081 cctttcctat gaaattttat ctgagcttca tttgcggtcg aatgatgagg atctggtaag
     1141 acgtgcgctt gaacgagtag gctatgatcc ggatgtggtc ggatatattc ttcccggaga
     1201 accaggagat aatggtggta gcggtggttc tgaattcgaa ataaattcct gtggctgtca
     1261 tgtttataag gagcaacgca tgccgggagg ctgtattcag gtcaaggatg ttgaaaagga
     1321 catttttcag ggcgtgagga gggttaaggt aattttgaaa gacacctggt ttaccgaaga
     1381 cgagacttgg actgacgaca atggctgttt tcggatcaac aacaaatatt atggcaaagc
     1441 atggatgtgg gtgaagttca aatcgccgag ggttcaaatc cggggtgcga gatcgggctt
     1501 tgcagcggtg tgggagtggg catttcctgt
//
LOCUS       DAOQMC010000378         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_65844_length_1517_cov_5.144323, whole genome shotgun sequence.
ACCESSION   DAOQMC010000378 DAOQMC010000000
VERSION     DAOQMC010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65844_length_1517_cov_5.144323"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1253)
                     /locus_tag="PKV31_10465"
     CDS             complement(102..1253)
                     /locus_tag="PKV31_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNB93327.1"
                     /translation="MIFQRRYILLFFTVIILVAVIYFLGDIVTYIIGAWVLALIGTPI
                     KKAILRIPGLSKWKYGNTIASTLTILFFLFLIGFGFFLIIPILSEQFSVFASIDYNKI
                     AATLQEPLSKVEIYLKKAGILQDSVSLLQEFSAEIERVLIPTKVSDFFNGIVSTVANI
                     GLAVLSILFIGFFFLKDQGLFDSGIRSFVPDKYEAAISKALDDIAMLLSRYFSGIIIQ
                     SCAIWLIISTSLSILGFSNAWLIGFFAGMVNVIPYLGPWIGAGFGVLVTINANINLDF
                     SQEILPMIYQLILVFGITHLIDNFLIYPLLFSNRVIAHPVEIFIVVILGAKIGGLIGM
                     IVAIPSYTIIRVLAGVFFSEFKVVQKITAGIRKATEDSPDQSPPTPKAE"
     gene            complement(1328..>1517)
                     /locus_tag="PKV31_10470"
     CDS             complement(1328..>1517)
                     /locus_tag="PKV31_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB93328.1"
                     /translation="VAPGFIKTDMTGDLDEAELVKLIPANRFGTPQEVAELVAFLAGD
                     KSGYITGQVISINGGLYS"
BASE COUNT          510 a          310 c          305 g          392 t
ORIGIN      
        1 aatattgatt tttaacatct tatgaaattt gtaaaattta agtgccgaag tcaggagcag
       61 ccagatacag atacatttat atcacccggt tatttgcact cctattctgc ctttggggtc
      121 ggagggctct gatccggtga atcctcggta gcttttctga ttccggcggt aattttttga
      181 acaaccttaa attcactgaa aaagacgccg gcaaggaccc tgataatggt atacgatggg
      241 atggctacaa tcatgccaat taaccctcca atcttggctc cgagaattac cacaatgaat
      301 atttccacag gatgagctat tacccggttg gaaaacaaca atgggtaaat caggaaatta
      361 tcgatcaaat gtgtaatccc gaatacaagg atcaattgat aaatcatcgg taggatctcc
      421 tggctgaaat ccagattaat atttgcatta atggtcacca gaacaccaaa accggcacca
      481 atccaagggc ccagataagg tatgacattg accattccgg caaagaatcc tatgagccag
      541 gcattggaaa atcccaaaat actcaaggaa gtggatatga taagccatat agcacagctc
      601 tggataatga tgccactaaa gtaccgcgac agaagcatgg ctatatcatc taatgccttt
      661 gaaatagcgg cttcgtactt atcgggcaca aaggatctaa taccactgtc aaacagaccc
      721 tgatccttca ggaagaaaaa tccgataaat agaatagaga ggacagcaag gccaatattg
      781 gctacagtac tgacaattcc attgaaaaaa tcagaaacct tggtaggaat caagacacgc
      841 tcaatttcag cagaaaattc ctgtaatagt gaaacactgt cctggagaat accggctttt
      901 ttcagataaa tctcaacttt cgacaagggc tcttgaaggg tggctgctat tttgttatag
      961 tcgattgagg caaatacact gaattgctcg gataatatgg ggataatcag gaaaaaacca
     1021 aatcctatca ggaacagaaa aaacaatatg gtcaatgtag aggcaattgt attgccatat
     1081 ttccacttac tgaggccggg tattcttagt atcgcttttt taatcggtgt gccaatcaag
     1141 gccagtaccc atgcgccaat gatataggta acgatgtcgc ccaagaaata gataacagca
     1201 accaaaatga tgactgtaaa aaataaaagt atatacctac gttgaaaaat cattggttgc
     1261 aggaggattg ataatctcct taattatgca aaggtaatta ttgtattcag aataaaccga
     1321 aaggatatta tgaatacaac ccgccgttaa tggaaatgac ctgaccggta atataacctg
     1381 atttatcacc agctaaaaat gcaaccaact cagctacttc ctgaggtgtc ccaaatcgat
     1441 tggcaggaat cagcttgact agttctgctt catctaagtc tccggtcata tctgtcttga
     1501 tgaagcccgg agcgact
//
LOCUS       DAOQMC010000379         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_65854_length_1517_cov_4.121751, whole genome shotgun sequence.
ACCESSION   DAOQMC010000379 DAOQMC010000000
VERSION     DAOQMC010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65854_length_1517_cov_4.121751"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKV31_10475"
     CDS             <1..201
                     /locus_tag="PKV31_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB93329.1"
                     /translation="LEKRKEMLKTNSYWNMKLIGMIYWGNNVDRFLDLNNVINKITVK
                     DIQETARKLFDGKNEFIGIMNP"
     gene            507..>1517
                     /locus_tag="PKV31_10480"
     CDS             507..>1517
                     /locus_tag="PKV31_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNB93330.1"
                     /translation="MRLLKMRHLLTVVIFLVTCFSAVAQDGNNKNALTFRALLADYFN
                     PNGGDLSDYKPYRYGYGFSYNRNLTSFLDVSVPFRFLNGRLKDQSTNTYVFGGDIQAQ
                     AKYYKPGNKVIPYILSGFGGDYIKDEGVNFQIPVGAGVDIRMFDRGYFNFNTEYRFSL
                     KTNRNNFIHGIGFKYLLGKKNKLADMDSDGVVDSLDLCPDIKGLAAFNGCPDSDNDGV
                     PDHLDECPDKPGSAKSKGCPDKDFDGIPDHLDKCPDQAGPIENQGCPVVDKDGDGIPD
                     TEDLCPDVPGMKSAMGCPDMDEDGVPDDKDECPDMPGLKIFKGCPDTDGDGVEDRYDR
                     CPD"
BASE COUNT          463 a          222 c          355 g          477 t
ORIGIN      
        1 ttggaaaagc gcaaggaaat gttaaaaacg aacagctatt ggaatatgaa attgattgga
       61 atgatatatt ggggcaataa tgtggatcga ttccttgatc tgaataacgt catcaacaaa
      121 attaccgtaa aagacataca agagactgcc agaaaattgt ttgatgggaa gaatgagttc
      181 attggcataa tgaatccgta aaaaatagaa atggaaaaga aggaataatc tgagaaaaat
      241 tgagagtgga actaattcac tctctttttt ctgaggtggt tcttttcctt agcagataaa
      301 atactgtgta cctgaatagg taatgtaatt ttagaattaa aaattattga atatttattt
      361 acatataata aaatatattt atatttttgc aatgcattgg cgtattcatc tgaatgctaa
      421 ttcgacagac gggttaatta ttggatggaa caaagagtat caccaatact gatttttgtc
      481 agaatggtta tttcgcaaaa aacaaaatga gactattaaa gatgaggcat cttttgaccg
      541 tagttatttt tttggtgact tgttttagtg cagttgccca agatggtaat aataaaaatg
      601 ctttgacctt cagagcactt ctggctgatt atttcaatcc caacggaggg gatttatcgg
      661 attataagcc ttatcgatac ggttatggat tttcttacaa caggaattta acttccttcc
      721 ttgacgtatc tgttccattt cgcttcctca atggccgttt gaaggatcaa tcaaccaata
      781 cctatgtttt tggtggtgac attcaggctc aagctaaata ttataaaccg ggcaataagg
      841 tcattcctta tattctatcg ggatttggag gtgattatat taaagatgaa ggagtgaatt
      901 ttcagattcc tgtcggtgcc ggtgtcgata taagaatgtt tgatcgcgga tattttaatt
      961 ttaatacaga gtatagattt tctcttaaga ctaatcggaa taattttatc catggtatag
     1021 gttttaaata cttgttggga aagaaaaata aattggccga tatggatagt gatggggttg
     1081 tggattcttt ggatctttgt ccggatatca aggggcttgc agcgttcaat ggttgtccgg
     1141 attctgataa tgatggtgtc cctgatcatc ttgatgagtg ccctgataag ccgggatctg
     1201 ccaaatcaaa gggttgtccg gataaggatt ttgatggtat tccggatcat ctggataaat
     1261 gtcctgatca ggcaggaccg attgagaacc agggctgtcc ggttgtggac aaggatggag
     1321 atggtatacc cgatactgaa gatttgtgtc ctgatgtgcc cggaatgaag tctgcaatgg
     1381 gatgccccga tatggatgaa gacggagtcc cggatgataa agatgaatgt cctgacatgc
     1441 ctggccttaa gattttcaaa ggctgtccgg atactgacgg ggatggggta gaggatagat
     1501 atgacaggtg tccggat
//
LOCUS       DAOQMC010000380         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0532
            NODE_66125_length_1514_cov_3.777930, whole genome shotgun sequence.
ACCESSION   DAOQMC010000380 DAOQMC010000000
VERSION     DAOQMC010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810164
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:16:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,097
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,076
            CDSs (with protein)               :: 2,076
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66125_length_1514_cov_3.777930"
                     /isolate="AMR_MDS_0532"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="PKV31_10485"
     CDS             complement(<1..1310)
                     /locus_tag="PKV31_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNB93331.1"
                     /translation="MTLDNFTINAQDAIIKAQKIASDRSQQTVDTAHILRGIIDTDES
                     VVSFLLNKMAVDLNPIKDENDKLIDLIPKVQGSDKQFLSNDANNAISKAKEAMKEFGD
                     EFISLELLFLGLFKGSDRTARLLKDRGLTEAGLVSAIKELRKGNKVNSQSGDSSYNSL
                     GKYAINLNERAESGKLDPIIGRDEEIRRVMHILSRRKKNNPILVGEAGVGKTAIVEGI
                     AWRIVKQDVPENLKSKTIFSLDLSSLVAGAKYKGEFEERLKAVIKEVTESDGEIILFI
                     DEIHTLIGAGGGSGAMDAANILKPALARGELHAIGATTLDEYQKYFEKDAALVRRFQN
                     VMVDEPSVEDTISILRGLQEKYEVFHKIEILDEALIAAAEMSHRYIADRQLPDKAIDL
                     IDEAAAKLRLELNSMPDSIDELDRKVRQLEIEREAIKREKDDKKL"
BASE COUNT          399 a          370 c          272 g          473 t
ORIGIN      
        1 agtttcttgt catctttttc tcgtttgatg gcttcacgtt ctatctcaag ctggcgtacc
       61 ttcctgtcca gttcatcgat tgaatcgggc atcgagttga gctccagtct tagcttggca
      121 gctgcttcat cgatcaggtc gatcgcctta tccggcaatt gtcgatctgc aatgtagcga
      181 tgagacattt ccgccgctgc aatcaacgct tcatccagaa tttcaatctt atggaagact
      241 tcatattttt cctgcagtcc tcgaagaatg gagatggtat cttccacact aggctcatct
      301 accatcacat tctgaaacct tcgtaccaaa gcagcatctt tttcaaaata cttctgatac
      361 tcatccaaag ttgtagcacc aatggcatgg agctcacctc ttgccaatgc aggcttaaga
      421 atattggcag catccattgc cccactgccc ccgcctgcac caatcaaggt gtggatctca
      481 tcaatgaaaa gtatgatttc tccatcgctt tctgtaacct ccttgatgac ggcttttagc
      541 ctttcctcaa attcaccctt atactttgca ccggcaacta atgatgatag gtccagactg
      601 aatatagtct tactcttcag gttttcaggc acatcttgct taacgatccg ccaggctata
      661 ccttcaacaa ttgcagtctt tcctacccct gcttcaccta ccaggatagg gttgttcttc
      721 tttcgacgtg aaagtatgtg cataacgcgt ctaatctctt catccctccc gatgataggg
      781 tcgagcttgc ccgattcagc cctttcattc agattaattg cgtatttacc aagtgaatta
      841 taggaactgt ctccgctctg agagttaacc ttattgccct ttctcaattc tttgatggca
      901 gagaccagtc ctgcttctgt cagcccgcga tcttttaaaa gtcttgcagt gcggtcgctt
      961 cccttgaata gacccaggaa aagcaattcc aaagaaatga attcgtcgcc aaactccttc
     1021 attgcctcct tcgcttttga gatcgcatta ttggcatcat tggaaagaaa ttgcttgtcc
     1081 gagccctgca ctttcggaat taaatcaatc aatttgtcat tttcatcttt aatgggatta
     1141 agatccactg ccattttatt caataaaaat gaaactacac tttcgtcagt gtcaatgatc
     1201 ccacgaagaa tatgagcggt atcaaccgtt tgctgagaac ggtcggatgc aattttttgc
     1261 gccttgatga tagcatcctg cgcatttatt gtgaaattat ccagtgtcat agttttcagt
     1321 tttacttgac ttgtatcaat caaaaaacaa gccaatataa gaactattct acttatatga
     1381 aaaaatggca ggcgcccaac gaagaacatg caattatgtc actgataacc atatgtttag
     1441 gacaaaatga caactgtgca actgcattac ggaatgctat accaagatga tttatataat
     1501 agtccataaa tttt
//
